LOC100183918 cDNA ORF clone, Ciona intestinalis(vase tunicate)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100183918
Entrez Gene ID	100183918
Full Name	uncharacterized LOC100183918
Gene Type	protein-coding
Organism	Ciona intestinalis(vase tunicate)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018813150.1	XP_018668695.1	uncharacterized protein LOC100183918

Pathways from BioSystems
Gene Ontology

The following LOC100183918 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100183918 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCi74420	XM_018813150.1 Latest version!	Ciona intestinalis uncharacterized LOC100183918 (LOC100183918), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100183918 ( XM_018813150.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_018668695.1 Ciona intestinalis uncharacterized LOC100183918 (LOC100183918), mRNA.

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CloneID	OCi74420
Clone ID Related Accession (Same CDS sequence)	XM_018813150.1
Accession Version	XM_018813150.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1476892800000
Organism	Ciona intestinalis(vase tunicate)
Product	uncharacterized protein LOC100183918
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020174.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ciona intestinalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 6% of CDS bases ##RefSeq-Attributes-END##

ATGAACCGCG GGTATTTCCT GGGACCCGAC CAGGGGAATA CCCTGAATTT ACTCACCGAT 
TGTGACCGCT TATGTCACGT GATTCACATT TTATATTTGG ATCGAATTAA ACAAGAACAA 
TACTCAGTGC CGCCGTACTT CTACAATCAC GTGGAAGACG TCACGTGCGG TTACGGTCAC 
GTGGCTACAT TGCATTGTGA CGTCACAGGG CAACCTGAAC CACGTGTCAG TTGGTTTAAA 
CAGGAACGTA ACGCCCCCCT ACGGGAAACC CCCGGGAAGT TTGCGCTACG TAAACAATGT 
GAGCGCGTGA CGCTTATCAT CTATGACGTC ACAACGGAAG ATATTGGATG TTATACATGC 
AAGATTTGGA ACGAACTTGG TGAAGCTCAA TCAACGGGGA GGTTGTTTTA TTCCCCCTCA 
CCAGATAGAA CAATCAACGA CAATGGGAGT TGTTTAAAGA AGGTCATCAT AAAAGAAACT 
GTGAAAATCG TAAAGCGTGA ACAAGATGCT CTCTCCTGCC ATGCGGTGGT GGCTCCAGGT 
TTCAACACTC AGCCACAAGA TGGGAGTAGA CGGGATTCAA TTACAATCCA CTTTCCACTG 
CTAGAGGGCA GTAGACTGAA GAACTTATCA GTGAGCACTG AGAGAAGCAA AGATAATAAA 
GAGAAAGGGA GGAGTGGGAG CAATAGGTGG CGTGGAGAAA CAGAAAGTGA TGTCTTCACG 
TCAACGAGCT CGTTTGACCT CGGATCGACC TCACCCGTGT CCAACAACGA GGATGTGTTC 
GACCAACCAA GCGTGACGAG TTCCCCCTCC CTTGATTCAT TCGACCAGGA TTTGATGAAA 
CGCGTTGAAT GGTTGGAACG TGGTAGGAAC TCAGGGTCGG AGTATCGTCG GGGTTCGGTT 
CCTTTTCATG CCTCCATTAT ACAACCTAGG GCGGATTCTG CCGACACTGG AATCTATTCG 
TTACAAAACG GAACAATGAA TGGGTCTCAT GATTGTGATG TCACAGAGGA GAAACAAGAC 
ACAATCACAA CGGAAACAAT GACAAGCCCC CTCCCCCCCA CATGGAGCCC GTCCAACATA 
AATGTTGGTT CGGGTTTTAT CGCTCGATCC GTTCGTGCAC AAAACCAAAA CCTATATTTT 
GATGGATTCT TCAACGGAAC CAATCAAGCA GGACCTGATG ATGTCATAAC CACTCAAGCA 
TGCCCCGATG ATGTCATAAT CACTCAAACG CAACATAATG ATGTCATAAC CACTCAAGCA 
CAAACTAATG ATGTCATAAC CACTCAAGCA CGTCCTGATG ATGTCATACC CACTCAAACA 
CGTCCTGATG ATGTCATAAC CACTCAAACA CGTCCTGATG ATGTCATAAC CACTCAAGCA 
TGCCCTGATG ATGTCATAAC CACTCAAGCA CAAACTAATG ACATCATAAC CACTCAAGCA 
TGCTCTGATG ATGTCATAAC CACTCAAGCA CAAACTAATG ATGTCATAAC CACTCAATCA 
CATCCTGATG ATGTCATAAT CAGTCGACCG CCACATAATA ATGTTACAAC CACCCAAGCA 
TGTCCTGATG ATGTCATAAC CACTCAAGCA GGACATGATG ATGTCAAAAT CACCCAAGTA 
TGTCCTGATG ATGTCATAAT CCCCCAACCC CAACATGATG ATGTCATAAC ACCTCTATTA 
AGCAACAATG GCATCGAAAT CGTTAACCTC CGCAATAGTG ATGATGTGGC GCAATCTGAT 
TCGTCGGTTC ATGTTGAATC ATGTGATTTA CTTGACCAAT CACAGTCAAG TAAAGCCAAT 
CCATCTATGA TGTCACAATG CAACGTATCT TCTATTGAAG CTTCAACATT TGATGTTGTG 
ACATCACAAN NNNNNNACAA TAAACCTTCG TTAACATCAC AAACACAAGA CAGTGTAACT 
ACTGTGATGT CACAAGTGAA GTGTCAAGAT GAAACAATTA ATGAAGAAGA ATGCTTTATT 
GTGACATCAT CGCAACAGGA AGTGACGTCA TTGCAACAGG AAGTGACATT ATCGCAACAG 
AAAGTGACAT CATCACAACA GGAAGTGACA TCATCACAAC ATGAAGTGAC ATCATCACAA 
CAGGAAGTGA CATCATCACA ACAGGAAGTG ACATCATCAC AACAGGAAGT GACATCATCA 
CAACAGGAAG TGACATCATC ACAACAGGAA GTGACATCAT TGCAACAGGA AGTGACATCA 
TTGCAACAAG AAGTGACATC ATCACAACAG GAAGTGAGAT CATTGCAACA GGAAGTGACA 
TCATCAGACA AAGTTGACCT GGATGATCTT ATTCATGAAT TGTTGGACTT CAGTGAAGCA 
ACGCCTGTTG TTGTGTCTTC GTTTATTGTT GGCTACCATA GAAATCAAAA AGTTACCGAC 
ATTGTCACAA TTGTACCCGA TCAGTGTAAA AATAAGACTC CAGTCATGAG GCGCTCTGCT 
TTGCCACCCA TTGGAAAGAA ACCTGAGATT TTAACGCCAC TAAAGGACGT TCACCTTCAT 
CGCGGGCAAG TGACGTCACT AAACGTATCG GTGACCGGCC AACCGGAACC GAAGATCACG 
TGGTTCAAGG ACGGCCAGCC ATTGGACGAG AACGCGATTG AAATCGACCA ATCAGAGCCG 
GCCAGTGGAG TTTACTCATG CGTGGCCACA AACAACCGTG GGGTGGCGAC CACGAGTGCT 
TGCGTCACAA TCAAACATCC GATGAAGAGG GTTCAATTCG GGGAGGAATT AACTCGATCA 
TATGATGAAC CCATGCAAGA TTATCTTTTA TACCAAGAAC CTCCAACACA GGAACCTCTT 
GACTGCTCCC TAGTGGATTA TCTTCGCAAC AAAGCCGACA GTCTTCCGTT CCACCATAGA 
CATCGTATAT CATTCAACGA AAGTGCCGAC TCGGGGGATG ACTCAGCTAC TTTTTCAGAG 
GACAATTCAT TGTCTCATGA TTATGACATC ACAAGGCGAT TCTCAATTGT TTCGTTCAGC 
AGCGTTTCAT CGGCAGCTTA CGTGACAGCA GATGAAGCAG GATCCGAAAC CACGCTTGAT 
TATTTTTCAC CAAACGAAGA TTTGATTTCC GCTGACGATT TCTTCAGCCC TGATTCGGAA 
CATTATTTAT CACAGGGAGA TTTACGAATG AAATCTTTAC CTCCACCAAG TGATGATAAT 
TTGTTCTGCC CACGACCTTT GACCCCAACA TTGATGATGT CATCACCCAA CATGATGACA 
TCACCATTTC AAATTACAGC GTTCGACACA ATTACCAACC AATCCTCCTC GGATGAAATG 
TTGACGGCCG ACAGCGGAGG GGAAACCCCT GTTCCGCAAC TCGATAGTGA CGATGATGAT 
GTCATAAAGC CACTATTGTT TGTGAGTGGA GTGCGTGATG TTTCAGCGAC GATTGGCACG 
AATGTGGAGT TGTCATGCAC CGTGGAGTGC CAACCAACAC CACGGGTCAT TTGGTATAAA 
AATAATCAAA AAATCCCCCA AACAGAGAAA GTTGCCAACA GGTTTTTGAC GGAAATCAAA 
CGAGAAAATA AAAACCAGTT CAAGATATCG TTGACCGTAC ACGATGTAAA ACCAGATGAT 
GGAGGGGAAT ATATGATGGC TGTCACTACA GCAGGTGGCA ACTTGTTCTC ATCAGGAAGT 
TTGATTATAG GGAGTTCCTT GGATTCGAAA CCTCATGCTA AATATGAACT GATGAGGGAA 
ATCCCTTCAA GTGTAAAATA CAGTTCCCCA CAAACTGGAA CTCCAAAAGT GTTTCCCCCC 
TCCTTTGATG ACATCACAGA TGACGTTGAA TGCAACATTG GCGACTCAGC AACTTTTCGA 
GTTACTGTGT TGGGAAATCC CCCACCGGTA GTTTCCTGGT ACAAGGATAA CGTTTTGTTG 
AACGAAGGCG AAATTGAAAG AATCGACGAA GGCAACCAAC ACAGCATCAT CATTCACGAT 
GTCACGCATG AACGATCGGG TATTTACTCA TGCACGGCGT CGAGCATCGC TGGTCATGTG 
ACCTGTAAAG CTGAGTTGGA GGCCAAGCTT GAACCTGATT GGTCGAAATC TGATGAAACT 
TCCCTCCAAA TTTCAAAACG AAGATTCAAA GATAATTACA AGGTAGATGA CATCATCGGT 
CGTGGGGTGA TTTCCCTCGT TAAGCGAGCA ACTCATCGTG TAACGAACCA CACGGTGGCA 
GCAAAGTTTA TACAGAACAA ACATAAAGAT CATATAACTG CTCAAAACGA AATTGACATT 
TTAACAAAGT GTCAGCACAA TCACATTGTG AAATTCTACG AAAGTTTCAC GACTTCTCGC 
CACATGATCA TTGTCACCGA AATTTGTAAA ATTGAATTGT TTGAGTTCTT TGTGACATCA 
CAATCCTTCT GTGAAGCAAT GGTATCACGA TACATGAAAC AACTTTGTGA AGCAATATCG 
TATCTACATA GAAACAATAT ACTTCATCTT AATGTTAAAC CCGAGAATAT TTTACTCGAT 
GAAAATTCAA CTTTAAAGTT GATTAACTTT GGAAGCGCTC ATCGTATGAA TGAGGATTCT 
GTTATTTATT GGAATCGCGG AACTCCAGAG TTTGTGGCTC CGGAAGTTGT AACCTCGTGC 
CAAGTGACAA CCGGCACTGA CATGTGGAGT GTGGGGGTTG TCACTTATCT GGCATTAACA 
GGGGTTTCCC CCTTTGCGGG AGAAACAGAC CAAATGACGT TGTTTCGGAT CCGTGATTTT 
GACGAAAAAC TAAATTTTAA ACATTTTCAT CAAATCTCGG AAAGTGGAAA AGATTTTGTA 
TCGATGTTGA TTGTGATGTC ACAATCCTCC CGATTGTCTG CTGATGATGT CATAAACCAT 
GAATGGATGG CATCATCAGT TGAGAAGAAA CTTGAAACAG AACGTTTGAA ATATTTTCAA 
TCAAGGAGGA AATGGCAGCG TTCGTTGCTC ACCGGTAAGT CAACCCTCAC AATCCGCTCT 
ATGACATCAC TATTACATGG GGAGTATGGT TCCACCTCAT TGGCCGTACC TCGTCATGTG 
ATCGATGATG TCACTTCCTC TACGACCGTT ACCGAGTCCG AGGATGAAGA GCCCCCACTG 
GTGCCCCGTC CCAACCCCAC CCCCACAATC CAAATATCGA GCCCCCTAAC TTCACCCGCC 
GTGGAAAGAA GGGAAATCTT CAAAGCAGGG AGAAGTTTGA GCGGGTCCAT GGAACGCCCC 
CTTCAACTTA AAGAGGATCG AAGCAAACCA CCGGAGGGCA GGAGATTGGA TTTGTTGCAA 
GTTGGTAAAA AGCAAGTGAG TTTTGAGGAA AAAGTTGACA TTGTGACATC ATCGGATGAC 
AATGTGTTTG ATGATGTCAT AAAGGAACCA CAAGAAAACA ATGAGTCGAA ACAGCATCAC 
CTAGTGGGAA GATCGGAGCC AGAGACGAAG AGAAAAAAGA AAACAAACAC TCGTGTCGTG 
CGTCATCGAT CATTTGACGT ACCCGTGTCC TCGTCTGATG AGGAAACGAG TGAAGTTAGG 
GGTAAAGAGG GGGTTTCCCA TCATGTAACC CCACACAAGG GTAAACTGGG GGTTCCCCAT 
GCTTCAACTG AAATAAGGGG TAAGGAGGGG AATCCCAGTC CCCCTCGGTG TTCGATCAAG 
CGGAGAATTT GGGGTCGGGC TGCTTCACTT GACAGCGAGC ATGGGGGAAT ACCCAAACCA 
AGAAAGGGGA TTTTAAAGAA GGGGAATTCC ACAGATTCAG AAACTTCTGG AAACCAGAAG 
TTTCGTCCCT TAAAGACGGG AGATGAGAAA TATCCCGATG TTGGAAACTA CAAAAGAAAA 
GTTATTCGTA AAGTGAATAC TCTGCCGGGC TCTGGTGCGC CACCTAGCAG CCCTCTTTCT 
TCCATATCTT CCGTCACAAG GAGGCGCCAG ATGATGGTCC GAGGATTGTC GGTTGATTCA 
GCAATCCGAC CCTCGATGGT AAAGGGAGGG TTACGGGGGC CATTGTTTGA ACAACGACAT 
CATAATGATG ATAGTGATGT CACAACAGCA GGGGCGATGA TGCGAGGAAG TCCGCTTCAA 
AATAGAAGTT TTTCTCCACT GAGGGGAAAC ACCCCCGACC CCCCAATAAT ATCCCGAGTC 
GTGGGGGCCG ATGTTTGGGT CAGGTGGTCA CCTTCCCATT GTGACTCAGC ACAAATAAGC 
TACCGTTTGG AAATGCAAAG TCATGGTTCA TCCTCGTGGA AAATTGTTGC TGAAAACATC 
AAATCAACCT CCTATCATGT GATCGGGCTA GATCGTCGTT TGTCGTATGT TTTCCGTGTC 
GCTGCCACCA CTAGTGGGCA ATATGGAGTG TTCAGTCAAC CGTCACAGAA TGTTTCGTTC 
ATGAAGAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018668695.1
CDS	1342..7716
Translation	MNRGYFLGPDQGNTLNLLTDCDRLCHVIHILYLDRIKQEQYSVPPYFYNHVEDVTCGYGHVATLHCDVTGQPEPRVSWFKQERNAPLRETPGKFALRKQCERVTLIIYDVTTEDIGCYTCKIWNELGEAQSTGRLFYSPSPDRTINDNGSCLKKVIIKETVKIVKREQDALSCHAVVAPGFNTQPQDGSRRDSITIHFPLLEGSRLKNLSVSTERSKDNKEKGRSGSNRWRGETESDVFTSTSSFDLGSTSPVSNNEDVFDQPSVTSSPSLDSFDQDLMKRVEWLERGRNSGSEYRRGSVPFHASIIQPRADSADTGIYSLQNGTMNGSHDCDVTEEKQDTITTETMTSPLPPTWSPSNINVGSGFIARSVRAQNQNLYFDGFFNGTNQAGPDDVITTQACPDDVIITQTQHNDVITTQAQTNDVITTQARPDDVIPTQTRPDDVITTQTRPDDVITTQACPDDVITTQAQTNDIITTQACSDDVITTQAQTNDVITTQSHPDDVIISRPPHNNVTTTQACPDDVITTQAGHDDVKITQVCPDDVIIPQPQHDDVITPLLSNNGIEIVNLRNSDDVAQSDSSVHVESCDLLDQSQSSKANPSMMSQCNVSSIEASTFDVVTSQnullnullnullNKPSLTSQTQDSVTTVMSQVKCQDETINEEECFIVTSSQQEVTSLQQEVTLSQQKVTSSQQEVTSSQHEVTSSQQEVTSSQQEVTSSQQEVTSSQQEVTSSQQEVTSLQQEVTSLQQEVTSSQQEVRSLQQEVTSSDKVDLDDLIHELLDFSEATPVVVSSFIVGYHRNQKVTDIVTIVPDQCKNKTPVMRRSALPPIGKKPEILTPLKDVHLHRGQVTSLNVSVTGQPEPKITWFKDGQPLDENAIEIDQSEPASGVYSCVATNNRGVATTSACVTIKHPMKRVQFGEELTRSYDEPMQDYLLYQEPPTQEPLDCSLVDYLRNKADSLPFHHRHRISFNESADSGDDSATFSEDNSLSHDYDITRRFSIVSFSSVSSAAYVTADEAGSETTLDYFSPNEDLISADDFFSPDSEHYLSQGDLRMKSLPPPSDDNLFCPRPLTPTLMMSSPNMMTSPFQITAFDTITNQSSSDEMLTADSGGETPVPQLDSDDDDVIKPLLFVSGVRDVSATIGTNVELSCTVECQPTPRVIWYKNNQKIPQTEKVANRFLTEIKRENKNQFKISLTVHDVKPDDGGEYMMAVTTAGGNLFSSGSLIIGSSLDSKPHAKYELMREIPSSVKYSSPQTGTPKVFPPSFDDITDDVECNIGDSATFRVTVLGNPPPVVSWYKDNVLLNEGEIERIDEGNQHSIIIHDVTHERSGIYSCTASSIAGHVTCKAELEAKLEPDWSKSDETSLQISKRRFKDNYKVDDIIGRGVISLVKRATHRVTNHTVAAKFIQNKHKDHITAQNEIDILTKCQHNHIVKFYESFTTSRHMIIVTEICKIELFEFFVTSQSFCEAMVSRYMKQLCEAISYLHRNNILHLNVKPENILLDENSTLKLINFGSAHRMNEDSVIYWNRGTPEFVAPEVVTSCQVTTGTDMWSVGVVTYLALTGVSPFAGETDQMTLFRIRDFDEKLNFKHFHQISESGKDFVSMLIVMSQSSRLSADDVINHEWMASSVEKKLETERLKYFQSRRKWQRSLLTGKSTLTIRSMTSLLHGEYGSTSLAVPRHVIDDVTSSTTVTESEDEEPPLVPRPNPTPTIQISSPLTSPAVERREIFKAGRSLSGSMERPLQLKEDRSKPPEGRRLDLLQVGKKQVSFEEKVDIVTSSDDNVFDDVIKEPQENNESKQHHLVGRSEPETKRKKKTNTRVVRHRSFDVPVSSSDEETSEVRGKEGVSHHVTPHKGKLGVPHASTEIRGKEGNPSPPRCSIKRRIWGRAASLDSEHGGIPKPRKGILKKGNSTDSETSGNQKFRPLKTGDEKYPDVGNYKRKVIRKVNTLPGSGAPPSSPLSSISSVTRRRQMMVRGLSVDSAIRPSMVKGGLRGPLFEQRHHNDDSDVTTAGAMMRGSPLQNRSFSPLRGNTPDPPIISRVVGADVWVRWSPSHCDSAQISYRLEMQSHGSSSWKIVAENIKSTSYHVIGLDRRLSYVFRVAATTSGQYGVFSQPSQNVSFMKKR

Target ORF information:

RefSeq Version	XM_018813150.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis uncharacterized LOC100183918 (LOC100183918), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018813150.1

ORF Insert Sequence:

ATGAACCGCG GGTATTTCCT GGGACCCGAC CAGGGGAATA CCCTGAATTT ACTCACCGAT 
TGTGACCGCT TATGTCACGT GATTCACATT TTATATTTGG ATCGAATTAA ACAAGAACAA 
TACTCAGTGC CGCCGTACTT CTACAATCAC GTGGAAGACG TCACGTGCGG TTACGGTCAC 
GTGGCTACAT TGCATTGTGA CGTCACAGGG CAACCTGAAC CACGTGTCAG TTGGTTTAAA 
CAGGAACGTA ACGCCCCCCT ACGGGAAACC CCCGGGAAGT TTGCGCTACG TAAACAATGT 
GAGCGCGTGA CGCTTATCAT CTATGACGTC ACAACGGAAG ATATTGGATG TTATACATGC 
AAGATTTGGA ACGAACTTGG TGAAGCTCAA TCAACGGGGA GGTTGTTTTA TTCCCCCTCA 
CCAGATAGAA CAATCAACGA CAATGGGAGT TGTTTAAAGA AGGTCATCAT AAAAGAAACT 
GTGAAAATCG TAAAGCGTGA ACAAGATGCT CTCTCCTGCC ATGCGGTGGT GGCTCCAGGT 
TTCAACACTC AGCCACAAGA TGGGAGTAGA CGGGATTCAA TTACAATCCA CTTTCCACTG 
CTAGAGGGCA GTAGACTGAA GAACTTATCA GTGAGCACTG AGAGAAGCAA AGATAATAAA 
GAGAAAGGGA GGAGTGGGAG CAATAGGTGG CGTGGAGAAA CAGAAAGTGA TGTCTTCACG 
TCAACGAGCT CGTTTGACCT CGGATCGACC TCACCCGTGT CCAACAACGA GGATGTGTTC 
GACCAACCAA GCGTGACGAG TTCCCCCTCC CTTGATTCAT TCGACCAGGA TTTGATGAAA 
CGCGTTGAAT GGTTGGAACG TGGTAGGAAC TCAGGGTCGG AGTATCGTCG GGGTTCGGTT 
CCTTTTCATG CCTCCATTAT ACAACCTAGG GCGGATTCTG CCGACACTGG AATCTATTCG 
TTACAAAACG GAACAATGAA TGGGTCTCAT GATTGTGATG TCACAGAGGA GAAACAAGAC 
ACAATCACAA CGGAAACAAT GACAAGCCCC CTCCCCCCCA CATGGAGCCC GTCCAACATA 
AATGTTGGTT CGGGTTTTAT CGCTCGATCC GTTCGTGCAC AAAACCAAAA CCTATATTTT 
GATGGATTCT TCAACGGAAC CAATCAAGCA GGACCTGATG ATGTCATAAC CACTCAAGCA 
TGCCCCGATG ATGTCATAAT CACTCAAACG CAACATAATG ATGTCATAAC CACTCAAGCA 
CAAACTAATG ATGTCATAAC CACTCAAGCA CGTCCTGATG ATGTCATACC CACTCAAACA 
CGTCCTGATG ATGTCATAAC CACTCAAACA CGTCCTGATG ATGTCATAAC CACTCAAGCA 
TGCCCTGATG ATGTCATAAC CACTCAAGCA CAAACTAATG ACATCATAAC CACTCAAGCA 
TGCTCTGATG ATGTCATAAC CACTCAAGCA CAAACTAATG ATGTCATAAC CACTCAATCA 
CATCCTGATG ATGTCATAAT CAGTCGACCG CCACATAATA ATGTTACAAC CACCCAAGCA 
TGTCCTGATG ATGTCATAAC CACTCAAGCA GGACATGATG ATGTCAAAAT CACCCAAGTA 
TGTCCTGATG ATGTCATAAT CCCCCAACCC CAACATGATG ATGTCATAAC ACCTCTATTA 
AGCAACAATG GCATCGAAAT CGTTAACCTC CGCAATAGTG ATGATGTGGC GCAATCTGAT 
TCGTCGGTTC ATGTTGAATC ATGTGATTTA CTTGACCAAT CACAGTCAAG TAAAGCCAAT 
CCATCTATGA TGTCACAATG CAACGTATCT TCTATTGAAG CTTCAACATT TGATGTTGTG 
ACATCACAAN NNNNNNACAA TAAACCTTCG TTAACATCAC AAACACAAGA CAGTGTAACT 
ACTGTGATGT CACAAGTGAA GTGTCAAGAT GAAACAATTA ATGAAGAAGA ATGCTTTATT 
GTGACATCAT CGCAACAGGA AGTGACGTCA TTGCAACAGG AAGTGACATT ATCGCAACAG 
AAAGTGACAT CATCACAACA GGAAGTGACA TCATCACAAC ATGAAGTGAC ATCATCACAA 
CAGGAAGTGA CATCATCACA ACAGGAAGTG ACATCATCAC AACAGGAAGT GACATCATCA 
CAACAGGAAG TGACATCATC ACAACAGGAA GTGACATCAT TGCAACAGGA AGTGACATCA 
TTGCAACAAG AAGTGACATC ATCACAACAG GAAGTGAGAT CATTGCAACA GGAAGTGACA 
TCATCAGACA AAGTTGACCT GGATGATCTT ATTCATGAAT TGTTGGACTT CAGTGAAGCA 
ACGCCTGTTG TTGTGTCTTC GTTTATTGTT GGCTACCATA GAAATCAAAA AGTTACCGAC 
ATTGTCACAA TTGTACCCGA TCAGTGTAAA AATAAGACTC CAGTCATGAG GCGCTCTGCT 
TTGCCACCCA TTGGAAAGAA ACCTGAGATT TTAACGCCAC TAAAGGACGT TCACCTTCAT 
CGCGGGCAAG TGACGTCACT AAACGTATCG GTGACCGGCC AACCGGAACC GAAGATCACG 
TGGTTCAAGG ACGGCCAGCC ATTGGACGAG AACGCGATTG AAATCGACCA ATCAGAGCCG 
GCCAGTGGAG TTTACTCATG CGTGGCCACA AACAACCGTG GGGTGGCGAC CACGAGTGCT 
TGCGTCACAA TCAAACATCC GATGAAGAGG GTTCAATTCG GGGAGGAATT AACTCGATCA 
TATGATGAAC CCATGCAAGA TTATCTTTTA TACCAAGAAC CTCCAACACA GGAACCTCTT 
GACTGCTCCC TAGTGGATTA TCTTCGCAAC AAAGCCGACA GTCTTCCGTT CCACCATAGA 
CATCGTATAT CATTCAACGA AAGTGCCGAC TCGGGGGATG ACTCAGCTAC TTTTTCAGAG 
GACAATTCAT TGTCTCATGA TTATGACATC ACAAGGCGAT TCTCAATTGT TTCGTTCAGC 
AGCGTTTCAT CGGCAGCTTA CGTGACAGCA GATGAAGCAG GATCCGAAAC CACGCTTGAT 
TATTTTTCAC CAAACGAAGA TTTGATTTCC GCTGACGATT TCTTCAGCCC TGATTCGGAA 
CATTATTTAT CACAGGGAGA TTTACGAATG AAATCTTTAC CTCCACCAAG TGATGATAAT 
TTGTTCTGCC CACGACCTTT GACCCCAACA TTGATGATGT CATCACCCAA CATGATGACA 
TCACCATTTC AAATTACAGC GTTCGACACA ATTACCAACC AATCCTCCTC GGATGAAATG 
TTGACGGCCG ACAGCGGAGG GGAAACCCCT GTTCCGCAAC TCGATAGTGA CGATGATGAT 
GTCATAAAGC CACTATTGTT TGTGAGTGGA GTGCGTGATG TTTCAGCGAC GATTGGCACG 
AATGTGGAGT TGTCATGCAC CGTGGAGTGC CAACCAACAC CACGGGTCAT TTGGTATAAA 
AATAATCAAA AAATCCCCCA AACAGAGAAA GTTGCCAACA GGTTTTTGAC GGAAATCAAA 
CGAGAAAATA AAAACCAGTT CAAGATATCG TTGACCGTAC ACGATGTAAA ACCAGATGAT 
GGAGGGGAAT ATATGATGGC TGTCACTACA GCAGGTGGCA ACTTGTTCTC ATCAGGAAGT 
TTGATTATAG GGAGTTCCTT GGATTCGAAA CCTCATGCTA AATATGAACT GATGAGGGAA 
ATCCCTTCAA GTGTAAAATA CAGTTCCCCA CAAACTGGAA CTCCAAAAGT GTTTCCCCCC 
TCCTTTGATG ACATCACAGA TGACGTTGAA TGCAACATTG GCGACTCAGC AACTTTTCGA 
GTTACTGTGT TGGGAAATCC CCCACCGGTA GTTTCCTGGT ACAAGGATAA CGTTTTGTTG 
AACGAAGGCG AAATTGAAAG AATCGACGAA GGCAACCAAC ACAGCATCAT CATTCACGAT 
GTCACGCATG AACGATCGGG TATTTACTCA TGCACGGCGT CGAGCATCGC TGGTCATGTG 
ACCTGTAAAG CTGAGTTGGA GGCCAAGCTT GAACCTGATT GGTCGAAATC TGATGAAACT 
TCCCTCCAAA TTTCAAAACG AAGATTCAAA GATAATTACA AGGTAGATGA CATCATCGGT 
CGTGGGGTGA TTTCCCTCGT TAAGCGAGCA ACTCATCGTG TAACGAACCA CACGGTGGCA 
GCAAAGTTTA TACAGAACAA ACATAAAGAT CATATAACTG CTCAAAACGA AATTGACATT 
TTAACAAAGT GTCAGCACAA TCACATTGTG AAATTCTACG AAAGTTTCAC GACTTCTCGC 
CACATGATCA TTGTCACCGA AATTTGTAAA ATTGAATTGT TTGAGTTCTT TGTGACATCA 
CAATCCTTCT GTGAAGCAAT GGTATCACGA TACATGAAAC AACTTTGTGA AGCAATATCG 
TATCTACATA GAAACAATAT ACTTCATCTT AATGTTAAAC CCGAGAATAT TTTACTCGAT 
GAAAATTCAA CTTTAAAGTT GATTAACTTT GGAAGCGCTC ATCGTATGAA TGAGGATTCT 
GTTATTTATT GGAATCGCGG AACTCCAGAG TTTGTGGCTC CGGAAGTTGT AACCTCGTGC 
CAAGTGACAA CCGGCACTGA CATGTGGAGT GTGGGGGTTG TCACTTATCT GGCATTAACA 
GGGGTTTCCC CCTTTGCGGG AGAAACAGAC CAAATGACGT TGTTTCGGAT CCGTGATTTT 
GACGAAAAAC TAAATTTTAA ACATTTTCAT CAAATCTCGG AAAGTGGAAA AGATTTTGTA 
TCGATGTTGA TTGTGATGTC ACAATCCTCC CGATTGTCTG CTGATGATGT CATAAACCAT 
GAATGGATGG CATCATCAGT TGAGAAGAAA CTTGAAACAG AACGTTTGAA ATATTTTCAA 
TCAAGGAGGA AATGGCAGCG TTCGTTGCTC ACCGGTAAGT CAACCCTCAC AATCCGCTCT 
ATGACATCAC TATTACATGG GGAGTATGGT TCCACCTCAT TGGCCGTACC TCGTCATGTG 
ATCGATGATG TCACTTCCTC TACGACCGTT ACCGAGTCCG AGGATGAAGA GCCCCCACTG 
GTGCCCCGTC CCAACCCCAC CCCCACAATC CAAATATCGA GCCCCCTAAC TTCACCCGCC 
GTGGAAAGAA GGGAAATCTT CAAAGCAGGG AGAAGTTTGA GCGGGTCCAT GGAACGCCCC 
CTTCAACTTA AAGAGGATCG AAGCAAACCA CCGGAGGGCA GGAGATTGGA TTTGTTGCAA 
GTTGGTAAAA AGCAAGTGAG TTTTGAGGAA AAAGTTGACA TTGTGACATC ATCGGATGAC 
AATGTGTTTG ATGATGTCAT AAAGGAACCA CAAGAAAACA ATGAGTCGAA ACAGCATCAC 
CTAGTGGGAA GATCGGAGCC AGAGACGAAG AGAAAAAAGA AAACAAACAC TCGTGTCGTG 
CGTCATCGAT CATTTGACGT ACCCGTGTCC TCGTCTGATG AGGAAACGAG TGAAGTTAGG 
GGTAAAGAGG GGGTTTCCCA TCATGTAACC CCACACAAGG GTAAACTGGG GGTTCCCCAT 
GCTTCAACTG AAATAAGGGG TAAGGAGGGG AATCCCAGTC CCCCTCGGTG TTCGATCAAG 
CGGAGAATTT GGGGTCGGGC TGCTTCACTT GACAGCGAGC ATGGGGGAAT ACCCAAACCA 
AGAAAGGGGA TTTTAAAGAA GGGGAATTCC ACAGATTCAG AAACTTCTGG AAACCAGAAG 
TTTCGTCCCT TAAAGACGGG AGATGAGAAA TATCCCGATG TTGGAAACTA CAAAAGAAAA 
GTTATTCGTA AAGTGAATAC TCTGCCGGGC TCTGGTGCGC CACCTAGCAG CCCTCTTTCT 
TCCATATCTT CCGTCACAAG GAGGCGCCAG ATGATGGTCC GAGGATTGTC GGTTGATTCA 
GCAATCCGAC CCTCGATGGT AAAGGGAGGG TTACGGGGGC CATTGTTTGA ACAACGACAT 
CATAATGATG ATAGTGATGT CACAACAGCA GGGGCGATGA TGCGAGGAAG TCCGCTTCAA 
AATAGAAGTT TTTCTCCACT GAGGGGAAAC ACCCCCGACC CCCCAATAAT ATCCCGAGTC 
GTGGGGGCCG ATGTTTGGGT CAGGTGGTCA CCTTCCCATT GTGACTCAGC ACAAATAAGC 
TACCGTTTGG AAATGCAAAG TCATGGTTCA TCCTCGTGGA AAATTGTTGC TGAAAACATC 
AAATCAACCT CCTATCATGT GATCGGGCTA GATCGTCGTT TGTCGTATGT TTTCCGTGTC 
GCTGCCACCA CTAGTGGGCA ATATGGAGTG TTCAGTCAAC CGTCACAGAA TGTTTCGTTC 
ATGAAGAAAA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100183918 cDNA ORF clone, Ciona intestinalis(vase tunicate)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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