Agl cDNA ORF clone, Cavia porcellus(domestic guinea pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	Agl
Entrez Gene ID	100721296
Full Name	amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase
Gene Type	protein-coding
Organism	Cavia porcellus(domestic guinea pig)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013142992.2	XP_012998446.1	glycogen debranching enzyme
XM_013142991.2	XP_012998445.1	glycogen debranching enzyme
XM_013142992.1	XP_012998446.1	glycogen debranching enzyme
XM_003479171.3	XP_003479219.1	glycogen debranching enzyme
XM_003479171.4	XP_003479219.1	glycogen debranching enzyme
XM_013142991.1	XP_012998445.1	glycogen debranching enzyme

Pathways from BioSystems
Gene Ontology

The following Agl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Agl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODo24114	XM_003479171.4 Latest version!	Cavia porcellus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODo24114	XM_013142991.2 Latest version!	Cavia porcellus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODo24114	XM_013142992.2 Latest version!	Cavia porcellus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
ODo24114	XM_013142992.1	Cavia porcellus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODo24114	XM_003479171.3	Cavia porcellus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODo24114	XM_013142991.1	Cavia porcellus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Agl ( XM_013142992.2 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_012998446.1 Cavia porcellus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODo24114
Clone ID Related Accession (Same CDS sequence)	XM_013142992.2 , XM_013142991.2 , XM_013142992.1 , XM_003479171.3 , XM_003479171.4 , XM_013142991.1
Accession Version	XM_013142992.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Cavia porcellus(domestic guinea pig)
Product	glycogen debranching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176417.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_013142992.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cavia porcellus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACACA GTAAACAGAT TCGAATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTTTTCA GACTTGAGCA AGGGTTTGAG CTACAGTTCC GATTGGGCCC AACTTTGCAG 
GGAAAACCAG TTACTGTATA TACAAATTAT CCATTTCCTG GAGAAACATT TAATCGACAT 
AAATTCCGTT CCCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAGCA GTCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAGAGTGGTG GAGGTTACAT AGTCGTGGAT CCTATTTTAC GAGTTGGTGC TGATAGCCAT 
GTGTTACCCT TGGATTGTGT TACTCTGCAG ACATTTTTAG CTAAGTGTTT AGGACCTTTT 
GATGAATGGG AAAGCAGAAT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCACTTC 
ACCCCGCTGC AGACTCTTGG ACTGTCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGATAGA AAGTACACCT GGCATGATGT TGGGCAACTG 
GTAGAAAAAC TGAAGAAGGA GTGGAATGTT CTTTGTATTA CAGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GCTCCAGGAA CACCCAGAGT CTGCATATAA TCTTGTGAAT 
TCTCCACATT TAAAACCGGC CTGGATTTTA GACAGAGCAC TTTGGCATTT CTCCTGTGAG 
GTTGCAGAAG GGAAATATAG AGAAAAAGGA ATCCCTGCTT TGATTGAAAA TGATCACCAC 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATGTTTACC CAAAGATTAA ACTGTGGGAA 
TTTTTCCAAG TACATGAACA CAGAGCAGTT GAGGAATTTA GAAGGCTGCT CACACAAGAA 
AATAGAAGAA CAACCAAGTC TGATCCAAAA CAACATCTTA AGATTATTCA GGATCCTGAA 
TATAGACGCC TTGGCTGTAC TATAGATATG AACATTGCAT TGGCAACTTT CATACCACAT 
AACAATGGAC CAGCCGCAAT TGAAGAATGC TGTAATTGGT TTCGTAAGAA AATTGAGGAA 
TTAAATTCAG AGAAATATCA ACTCATACAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGAATGGCT GGCCATGGTC CTAAACTAGG ACCTGTCACT 
AGAAAACATC CCTTAGTTAC CAGGTATTTT ACTTTCCCAT TCCAAGAAAT GGACTTATCC 
ACAGAAGAGT CCATGATGCA CCTCCCTGAT AAAGCTTGTT TCTTCATGGC ACATAATGGA 
TGGGTGATGG GAGATGACCC TCTTCGAAAC TTTGCTGAGC CAGGTTCAGA TGTTTACTTA 
AGGCGAGAAC TTATATGCTG GGGCGACAGT GTTAAATTGC GCTACGGAAA TAAGCCAGAG 
GATTGTCCTT ACCTATGGGC ACACATGAAA AAATACACTG AAATCACTGC GACTTATTTC 
CAGGGTGTAC GTCTTGATAA CTGCCACTCA ACACCTATTC ATGTGGCTGA GTACATGTTG 
GATGCTGCTA GGAAATTACA ACCCAATTTA TATGTAGTAG CTGAACTATT CACAGGAAGT 
GAAGAGCTGG ACAATATCTT TGTCACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCG 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG TGGAGAACCG 
GTTGGATCCT TTGTTCAGCC CTGTCTGAGG CCTTTAATGC CAGCTATCGC ACATGCCCTG 
TTTATGGATA TTACCCATGA TAACGAGTGT CCTATTGTGC ATAGATCAGC CTACGATGCT 
CTTCCAAGTA CCACAATTGT GTCTATGGCA TCTTGTGCTA GTGGAAGTAC ACGAGGCTAT 
GATGAATTAG TGCCTCATCA GATATCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGGAG CATTGCCATC AAATGTGGGT GATGTTAATT TCCAGAGTGG AATTATTGCA 
GCCAGATGTG CTATCAATAA ACTTCATCAA GAGCTCGGAG CCAAAGGTTT TATTCAGGTA 
TATGTAGATC AAGTTGATGA AGACATAGTG GCAGTGACCA GACATTCACC TAGTATCCAT 
CAGTCCGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAAACTTC GTTTTACAGC 
AAGGAAGTAC CTCAGATGTG CATCCCTGGC AAAATTGAAG AGGTTGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAACACACA ACCATATGTA AAGGATGAGC ATTCAATCAA CGGGCTGCCA 
AACATCACAG TAGAAATTAG GGAACATATT CAGCTCAAAG AAAGTAAAAT TGTAAAACAA 
GCTGGAATTG CCACAAAAGG TCCAAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATCGT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GGAACCATCT GACACAGTTC AGTCCGCACT TTAAATCTGG AAGCCTTGCT 
GTTGACAACT CGGATCCTAT TTTGAAAATC CCATTTGCTT CTATTGCCTC TAACTTGACT 
TTGGCTGAGC TAAATCAAAT ACTTTACCGA TGTGAATCAG AGGAACAAGA AGATGGTGGA 
GGTTGTTACA ACATACCAAA CTGGTCATCC CTTAAATACG CAGGTCTTCA AGGATTAATG 
TCTGTATTGG CAGAAATTAG ACCAAAGAAT GATTTGGGGC ATCCTTTTTG TGATAATTTG 
AGATCCGGAG ATTGGATGAT TGACTATGTC AGTGGCCGGC TTATTTCACG ATCAGGAAAT 
ATTGCTGAAG TCGGCAAATG GTTACAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGA 
TATCTTATCC CATGTTATTT TGATGCAATA TTAATTGGTG TATATACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACATTTGT AAAACACCTT 
TCATTGGGGT CAGTTCAGAT GTGTGGAGTA GGAAAATTCC CTTGTCTGCC CCTTCTGTCT 
CCTTCACTTT GGGATGTGCC ACACAGGGTA AATGAGATCA CAAAAGAAAA GGAGCAGTGT 
TGTGCTTCTC TGGCTGCAGG CTTACCTCAT TTTTCTGCTG GTCTTTTCCG CTGTTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTCTT TTGCTGGTTA CTGGACGCTA CTTAGAGGCC 
AGGAACATTA TTTTAGCATT CGCTGGTACT CTGAGGCATG GTCTTATCCC CAACCTCCTT 
GGTGAAGGAG TTTATGCCAG GTACAATTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGATT ACTGTAAGAT CGTCCCGAAC GGTCTAGACA TTCTCAAGTG TCCAGTGTCC 
AGGATGTATC CAACAGATGA TTCTGCTCCT TTGCCTGCTG GTACACTGGA TCAACCACTA 
TTTGAAGTGA TACAAGAAAC TATGCAAAGA CATATGCAGG GCATACAGTT CCGAGAGAGA 
AATGCTGGCT CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT AACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGT GGAAATCGCT TCAATTGTGG CACGTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TGGAAATTGT AGGCCTTGCC AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTTCC TTATCATGAA GTTAGAATAA AAAGACACGG GAAAGTTGTA 
GCCGTCTCAT ATGATGAGTG GAACAGGAAA ATACAAGACA ACTTTGAAAA GCTGTTTCAT 
GTTTCGGAAG ACCCTTCAGA CCCTAATGAG AAGCATCCAA ATCTAGTCCA CAAACGGGGC 
ATCTACAAAG ATAGTTACGG AGCTTCCAGT CCTTGGTGTG ACTACCAGCT CAGGCCGAAC 
TTTACCATCG CAATGGTCGT GGCCCCTGAG CTCTTTACTG CAGGAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTGGGC CCCCTAGGCA TGAAAACCTT AGATCCAGAT 
GATATGGTGT ACTGTGGAAT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AGAGGTTTCA ATTATCATCA AGGACCTGAG TGGCTATGGC CTGTTGGATA TTTCCTTCGT 
GCAAAGTTAT ATTTTGCCAA ATTGATGGGT CCAGAGACTA CTGCAAAAAC TGTGTTTTTG 
GTCAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCTATTG GAAAGGTCTT 
CCAGAACTGA CCAATGAAAA TGGCCAATAC TGTCCTTTCA GCTGTGAGAC ACAAGCTTGG 
TCAATTGCTA CCGTTCTTGA AACACTTTAT GATTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012998446.1
CDS	178..4776
Translation	MEHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKPVTVYTNYPFPGETFNRHKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADSHVLPLDCVTLQTFLAKCLGPFDEWESRIRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPDRKYTWHDVGQLVEKLKKEWNVLCITDVVYNHTAANSKWLQEHPESAYNLVNSPHLKPAWILDRALWHFSCEVAEGKYREKGIPALIENDHHMNCIRKVIWEDVYPKIKLWEFFQVHEHRAVEEFRRLLTQENRRTTKSDPKQHLKIIQDPEYRRLGCTIDMNIALATFIPHNNGPAAIEECCNWFRKKIEELNSEKYQLIHYHQEQAVNCLLGNVFYERMAGHGPKLGPVTRKHPLVTRYFTFPFQEMDLSTEESMMHLPDKACFFMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPIHVAEYMLDAARKLQPNLYVVAELFTGSEELDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMASCASGSTRGYDELVPHQISVVSEERFYTKWNPGALPSNVGDVNFQSGIIAARCAINKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTVERNTQPYVKDEHSINGLPNITVEIREHIQLKESKIVKQAGIATKGPNEYIQEIEFENLSPGSVIVFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNSDPILKIPFASIASNLTLAELNQILYRCESEEQEDGGGCYNIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSGRLISRSGNIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGVYTTLLDIAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGKFPCLPLLSPSLWDVPHRVNEITKEKEQCCASLAAGLPHFSAGLFRCWGRDTFIALRGLLLVTGRYLEARNIILAFAGTLRHGLIPNLLGEGVYARYNCRDAVWWWLQCIQDYCKIVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQETMQRHMQGIQFRERNAGSQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLAKSAVRWLLELSKKNIFPYHEVRIKRHGKVVAVSYDEWNRKIQDNFEKLFHVSEDPSDPNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAGKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLARGFNYHQGPEWLWPVGYFLRAKLYFAKLMGPETTAKTVFLVKNVLSRHYVHLERSYWKGLPELTNENGQYCPFSCETQAWSIATVLETLYDL

Target ORF information:

RefSeq Version	XM_013142992.2
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013142992.2

ORF Insert Sequence:

ATGGAACACA GTAAACAGAT TCGAATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTTTTCA GACTTGAGCA AGGGTTTGAG CTACAGTTCC GATTGGGCCC AACTTTGCAG 
GGAAAACCAG TTACTGTATA TACAAATTAT CCATTTCCTG GAGAAACATT TAATCGACAT 
AAATTCCGTT CCCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAGCA GTCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAGAGTGGTG GAGGTTACAT AGTCGTGGAT CCTATTTTAC GAGTTGGTGC TGATAGCCAT 
GTGTTACCCT TGGATTGTGT TACTCTGCAG ACATTTTTAG CTAAGTGTTT AGGACCTTTT 
GATGAATGGG AAAGCAGAAT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCACTTC 
ACCCCGCTGC AGACTCTTGG ACTGTCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGATAGA AAGTACACCT GGCATGATGT TGGGCAACTG 
GTAGAAAAAC TGAAGAAGGA GTGGAATGTT CTTTGTATTA CAGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GCTCCAGGAA CACCCAGAGT CTGCATATAA TCTTGTGAAT 
TCTCCACATT TAAAACCGGC CTGGATTTTA GACAGAGCAC TTTGGCATTT CTCCTGTGAG 
GTTGCAGAAG GGAAATATAG AGAAAAAGGA ATCCCTGCTT TGATTGAAAA TGATCACCAC 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATGTTTACC CAAAGATTAA ACTGTGGGAA 
TTTTTCCAAG TACATGAACA CAGAGCAGTT GAGGAATTTA GAAGGCTGCT CACACAAGAA 
AATAGAAGAA CAACCAAGTC TGATCCAAAA CAACATCTTA AGATTATTCA GGATCCTGAA 
TATAGACGCC TTGGCTGTAC TATAGATATG AACATTGCAT TGGCAACTTT CATACCACAT 
AACAATGGAC CAGCCGCAAT TGAAGAATGC TGTAATTGGT TTCGTAAGAA AATTGAGGAA 
TTAAATTCAG AGAAATATCA ACTCATACAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGAATGGCT GGCCATGGTC CTAAACTAGG ACCTGTCACT 
AGAAAACATC CCTTAGTTAC CAGGTATTTT ACTTTCCCAT TCCAAGAAAT GGACTTATCC 
ACAGAAGAGT CCATGATGCA CCTCCCTGAT AAAGCTTGTT TCTTCATGGC ACATAATGGA 
TGGGTGATGG GAGATGACCC TCTTCGAAAC TTTGCTGAGC CAGGTTCAGA TGTTTACTTA 
AGGCGAGAAC TTATATGCTG GGGCGACAGT GTTAAATTGC GCTACGGAAA TAAGCCAGAG 
GATTGTCCTT ACCTATGGGC ACACATGAAA AAATACACTG AAATCACTGC GACTTATTTC 
CAGGGTGTAC GTCTTGATAA CTGCCACTCA ACACCTATTC ATGTGGCTGA GTACATGTTG 
GATGCTGCTA GGAAATTACA ACCCAATTTA TATGTAGTAG CTGAACTATT CACAGGAAGT 
GAAGAGCTGG ACAATATCTT TGTCACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCG 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG TGGAGAACCG 
GTTGGATCCT TTGTTCAGCC CTGTCTGAGG CCTTTAATGC CAGCTATCGC ACATGCCCTG 
TTTATGGATA TTACCCATGA TAACGAGTGT CCTATTGTGC ATAGATCAGC CTACGATGCT 
CTTCCAAGTA CCACAATTGT GTCTATGGCA TCTTGTGCTA GTGGAAGTAC ACGAGGCTAT 
GATGAATTAG TGCCTCATCA GATATCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGGAG CATTGCCATC AAATGTGGGT GATGTTAATT TCCAGAGTGG AATTATTGCA 
GCCAGATGTG CTATCAATAA ACTTCATCAA GAGCTCGGAG CCAAAGGTTT TATTCAGGTA 
TATGTAGATC AAGTTGATGA AGACATAGTG GCAGTGACCA GACATTCACC TAGTATCCAT 
CAGTCCGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAAACTTC GTTTTACAGC 
AAGGAAGTAC CTCAGATGTG CATCCCTGGC AAAATTGAAG AGGTTGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAACACACA ACCATATGTA AAGGATGAGC ATTCAATCAA CGGGCTGCCA 
AACATCACAG TAGAAATTAG GGAACATATT CAGCTCAAAG AAAGTAAAAT TGTAAAACAA 
GCTGGAATTG CCACAAAAGG TCCAAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATCGT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GGAACCATCT GACACAGTTC AGTCCGCACT TTAAATCTGG AAGCCTTGCT 
GTTGACAACT CGGATCCTAT TTTGAAAATC CCATTTGCTT CTATTGCCTC TAACTTGACT 
TTGGCTGAGC TAAATCAAAT ACTTTACCGA TGTGAATCAG AGGAACAAGA AGATGGTGGA 
GGTTGTTACA ACATACCAAA CTGGTCATCC CTTAAATACG CAGGTCTTCA AGGATTAATG 
TCTGTATTGG CAGAAATTAG ACCAAAGAAT GATTTGGGGC ATCCTTTTTG TGATAATTTG 
AGATCCGGAG ATTGGATGAT TGACTATGTC AGTGGCCGGC TTATTTCACG ATCAGGAAAT 
ATTGCTGAAG TCGGCAAATG GTTACAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGA 
TATCTTATCC CATGTTATTT TGATGCAATA TTAATTGGTG TATATACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACATTTGT AAAACACCTT 
TCATTGGGGT CAGTTCAGAT GTGTGGAGTA GGAAAATTCC CTTGTCTGCC CCTTCTGTCT 
CCTTCACTTT GGGATGTGCC ACACAGGGTA AATGAGATCA CAAAAGAAAA GGAGCAGTGT 
TGTGCTTCTC TGGCTGCAGG CTTACCTCAT TTTTCTGCTG GTCTTTTCCG CTGTTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTCTT TTGCTGGTTA CTGGACGCTA CTTAGAGGCC 
AGGAACATTA TTTTAGCATT CGCTGGTACT CTGAGGCATG GTCTTATCCC CAACCTCCTT 
GGTGAAGGAG TTTATGCCAG GTACAATTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGATT ACTGTAAGAT CGTCCCGAAC GGTCTAGACA TTCTCAAGTG TCCAGTGTCC 
AGGATGTATC CAACAGATGA TTCTGCTCCT TTGCCTGCTG GTACACTGGA TCAACCACTA 
TTTGAAGTGA TACAAGAAAC TATGCAAAGA CATATGCAGG GCATACAGTT CCGAGAGAGA 
AATGCTGGCT CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT AACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGT GGAAATCGCT TCAATTGTGG CACGTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TGGAAATTGT AGGCCTTGCC AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTTCC TTATCATGAA GTTAGAATAA AAAGACACGG GAAAGTTGTA 
GCCGTCTCAT ATGATGAGTG GAACAGGAAA ATACAAGACA ACTTTGAAAA GCTGTTTCAT 
GTTTCGGAAG ACCCTTCAGA CCCTAATGAG AAGCATCCAA ATCTAGTCCA CAAACGGGGC 
ATCTACAAAG ATAGTTACGG AGCTTCCAGT CCTTGGTGTG ACTACCAGCT CAGGCCGAAC 
TTTACCATCG CAATGGTCGT GGCCCCTGAG CTCTTTACTG CAGGAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTGGGC CCCCTAGGCA TGAAAACCTT AGATCCAGAT 
GATATGGTGT ACTGTGGAAT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AGAGGTTTCA ATTATCATCA AGGACCTGAG TGGCTATGGC CTGTTGGATA TTTCCTTCGT 
GCAAAGTTAT ATTTTGCCAA ATTGATGGGT CCAGAGACTA CTGCAAAAAC TGTGTTTTTG 
GTCAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCTATTG GAAAGGTCTT 
CCAGAACTGA CCAATGAAAA TGGCCAATAC TGTCCTTTCA GCTGTGAGAC ACAAGCTTGG 
TCAATTGCTA CCGTTCTTGA AACACTTTAT GATTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agl ( XM_013142991.2 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_012998445.1 Cavia porcellus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODo24114
Clone ID Related Accession (Same CDS sequence)	XM_013142992.2 , XM_013142991.2 , XM_013142992.1 , XM_003479171.3 , XM_003479171.4 , XM_013142991.1
Accession Version	XM_013142991.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Cavia porcellus(domestic guinea pig)
Product	glycogen debranching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176417.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_013142991.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cavia porcellus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACACA GTAAACAGAT TCGAATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTTTTCA GACTTGAGCA AGGGTTTGAG CTACAGTTCC GATTGGGCCC AACTTTGCAG 
GGAAAACCAG TTACTGTATA TACAAATTAT CCATTTCCTG GAGAAACATT TAATCGACAT 
AAATTCCGTT CCCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAGCA GTCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAGAGTGGTG GAGGTTACAT AGTCGTGGAT CCTATTTTAC GAGTTGGTGC TGATAGCCAT 
GTGTTACCCT TGGATTGTGT TACTCTGCAG ACATTTTTAG CTAAGTGTTT AGGACCTTTT 
GATGAATGGG AAAGCAGAAT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCACTTC 
ACCCCGCTGC AGACTCTTGG ACTGTCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGATAGA AAGTACACCT GGCATGATGT TGGGCAACTG 
GTAGAAAAAC TGAAGAAGGA GTGGAATGTT CTTTGTATTA CAGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GCTCCAGGAA CACCCAGAGT CTGCATATAA TCTTGTGAAT 
TCTCCACATT TAAAACCGGC CTGGATTTTA GACAGAGCAC TTTGGCATTT CTCCTGTGAG 
GTTGCAGAAG GGAAATATAG AGAAAAAGGA ATCCCTGCTT TGATTGAAAA TGATCACCAC 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATGTTTACC CAAAGATTAA ACTGTGGGAA 
TTTTTCCAAG TACATGAACA CAGAGCAGTT GAGGAATTTA GAAGGCTGCT CACACAAGAA 
AATAGAAGAA CAACCAAGTC TGATCCAAAA CAACATCTTA AGATTATTCA GGATCCTGAA 
TATAGACGCC TTGGCTGTAC TATAGATATG AACATTGCAT TGGCAACTTT CATACCACAT 
AACAATGGAC CAGCCGCAAT TGAAGAATGC TGTAATTGGT TTCGTAAGAA AATTGAGGAA 
TTAAATTCAG AGAAATATCA ACTCATACAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGAATGGCT GGCCATGGTC CTAAACTAGG ACCTGTCACT 
AGAAAACATC CCTTAGTTAC CAGGTATTTT ACTTTCCCAT TCCAAGAAAT GGACTTATCC 
ACAGAAGAGT CCATGATGCA CCTCCCTGAT AAAGCTTGTT TCTTCATGGC ACATAATGGA 
TGGGTGATGG GAGATGACCC TCTTCGAAAC TTTGCTGAGC CAGGTTCAGA TGTTTACTTA 
AGGCGAGAAC TTATATGCTG GGGCGACAGT GTTAAATTGC GCTACGGAAA TAAGCCAGAG 
GATTGTCCTT ACCTATGGGC ACACATGAAA AAATACACTG AAATCACTGC GACTTATTTC 
CAGGGTGTAC GTCTTGATAA CTGCCACTCA ACACCTATTC ATGTGGCTGA GTACATGTTG 
GATGCTGCTA GGAAATTACA ACCCAATTTA TATGTAGTAG CTGAACTATT CACAGGAAGT 
GAAGAGCTGG ACAATATCTT TGTCACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCG 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG TGGAGAACCG 
GTTGGATCCT TTGTTCAGCC CTGTCTGAGG CCTTTAATGC CAGCTATCGC ACATGCCCTG 
TTTATGGATA TTACCCATGA TAACGAGTGT CCTATTGTGC ATAGATCAGC CTACGATGCT 
CTTCCAAGTA CCACAATTGT GTCTATGGCA TCTTGTGCTA GTGGAAGTAC ACGAGGCTAT 
GATGAATTAG TGCCTCATCA GATATCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGGAG CATTGCCATC AAATGTGGGT GATGTTAATT TCCAGAGTGG AATTATTGCA 
GCCAGATGTG CTATCAATAA ACTTCATCAA GAGCTCGGAG CCAAAGGTTT TATTCAGGTA 
TATGTAGATC AAGTTGATGA AGACATAGTG GCAGTGACCA GACATTCACC TAGTATCCAT 
CAGTCCGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAAACTTC GTTTTACAGC 
AAGGAAGTAC CTCAGATGTG CATCCCTGGC AAAATTGAAG AGGTTGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAACACACA ACCATATGTA AAGGATGAGC ATTCAATCAA CGGGCTGCCA 
AACATCACAG TAGAAATTAG GGAACATATT CAGCTCAAAG AAAGTAAAAT TGTAAAACAA 
GCTGGAATTG CCACAAAAGG TCCAAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATCGT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GGAACCATCT GACACAGTTC AGTCCGCACT TTAAATCTGG AAGCCTTGCT 
GTTGACAACT CGGATCCTAT TTTGAAAATC CCATTTGCTT CTATTGCCTC TAACTTGACT 
TTGGCTGAGC TAAATCAAAT ACTTTACCGA TGTGAATCAG AGGAACAAGA AGATGGTGGA 
GGTTGTTACA ACATACCAAA CTGGTCATCC CTTAAATACG CAGGTCTTCA AGGATTAATG 
TCTGTATTGG CAGAAATTAG ACCAAAGAAT GATTTGGGGC ATCCTTTTTG TGATAATTTG 
AGATCCGGAG ATTGGATGAT TGACTATGTC AGTGGCCGGC TTATTTCACG ATCAGGAAAT 
ATTGCTGAAG TCGGCAAATG GTTACAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGA 
TATCTTATCC CATGTTATTT TGATGCAATA TTAATTGGTG TATATACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACATTTGT AAAACACCTT 
TCATTGGGGT CAGTTCAGAT GTGTGGAGTA GGAAAATTCC CTTGTCTGCC CCTTCTGTCT 
CCTTCACTTT GGGATGTGCC ACACAGGGTA AATGAGATCA CAAAAGAAAA GGAGCAGTGT 
TGTGCTTCTC TGGCTGCAGG CTTACCTCAT TTTTCTGCTG GTCTTTTCCG CTGTTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTCTT TTGCTGGTTA CTGGACGCTA CTTAGAGGCC 
AGGAACATTA TTTTAGCATT CGCTGGTACT CTGAGGCATG GTCTTATCCC CAACCTCCTT 
GGTGAAGGAG TTTATGCCAG GTACAATTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGATT ACTGTAAGAT CGTCCCGAAC GGTCTAGACA TTCTCAAGTG TCCAGTGTCC 
AGGATGTATC CAACAGATGA TTCTGCTCCT TTGCCTGCTG GTACACTGGA TCAACCACTA 
TTTGAAGTGA TACAAGAAAC TATGCAAAGA CATATGCAGG GCATACAGTT CCGAGAGAGA 
AATGCTGGCT CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT AACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGT GGAAATCGCT TCAATTGTGG CACGTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TGGAAATTGT AGGCCTTGCC AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTTCC TTATCATGAA GTTAGAATAA AAAGACACGG GAAAGTTGTA 
GCCGTCTCAT ATGATGAGTG GAACAGGAAA ATACAAGACA ACTTTGAAAA GCTGTTTCAT 
GTTTCGGAAG ACCCTTCAGA CCCTAATGAG AAGCATCCAA ATCTAGTCCA CAAACGGGGC 
ATCTACAAAG ATAGTTACGG AGCTTCCAGT CCTTGGTGTG ACTACCAGCT CAGGCCGAAC 
TTTACCATCG CAATGGTCGT GGCCCCTGAG CTCTTTACTG CAGGAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTGGGC CCCCTAGGCA TGAAAACCTT AGATCCAGAT 
GATATGGTGT ACTGTGGAAT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AGAGGTTTCA ATTATCATCA AGGACCTGAG TGGCTATGGC CTGTTGGATA TTTCCTTCGT 
GCAAAGTTAT ATTTTGCCAA ATTGATGGGT CCAGAGACTA CTGCAAAAAC TGTGTTTTTG 
GTCAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCTATTG GAAAGGTCTT 
CCAGAACTGA CCAATGAAAA TGGCCAATAC TGTCCTTTCA GCTGTGAGAC ACAAGCTTGG 
TCAATTGCTA CCGTTCTTGA AACACTTTAT GATTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012998445.1
CDS	307..4905
Translation	MEHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKPVTVYTNYPFPGETFNRHKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADSHVLPLDCVTLQTFLAKCLGPFDEWESRIRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPDRKYTWHDVGQLVEKLKKEWNVLCITDVVYNHTAANSKWLQEHPESAYNLVNSPHLKPAWILDRALWHFSCEVAEGKYREKGIPALIENDHHMNCIRKVIWEDVYPKIKLWEFFQVHEHRAVEEFRRLLTQENRRTTKSDPKQHLKIIQDPEYRRLGCTIDMNIALATFIPHNNGPAAIEECCNWFRKKIEELNSEKYQLIHYHQEQAVNCLLGNVFYERMAGHGPKLGPVTRKHPLVTRYFTFPFQEMDLSTEESMMHLPDKACFFMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPIHVAEYMLDAARKLQPNLYVVAELFTGSEELDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMASCASGSTRGYDELVPHQISVVSEERFYTKWNPGALPSNVGDVNFQSGIIAARCAINKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTVERNTQPYVKDEHSINGLPNITVEIREHIQLKESKIVKQAGIATKGPNEYIQEIEFENLSPGSVIVFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNSDPILKIPFASIASNLTLAELNQILYRCESEEQEDGGGCYNIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSGRLISRSGNIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGVYTTLLDIAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGKFPCLPLLSPSLWDVPHRVNEITKEKEQCCASLAAGLPHFSAGLFRCWGRDTFIALRGLLLVTGRYLEARNIILAFAGTLRHGLIPNLLGEGVYARYNCRDAVWWWLQCIQDYCKIVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQETMQRHMQGIQFRERNAGSQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLAKSAVRWLLELSKKNIFPYHEVRIKRHGKVVAVSYDEWNRKIQDNFEKLFHVSEDPSDPNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAGKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLARGFNYHQGPEWLWPVGYFLRAKLYFAKLMGPETTAKTVFLVKNVLSRHYVHLERSYWKGLPELTNENGQYCPFSCETQAWSIATVLETLYDL

Target ORF information:

RefSeq Version	XM_013142991.2
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013142991.2

ORF Insert Sequence:

ATGGAACACA GTAAACAGAT TCGAATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTTTTCA GACTTGAGCA AGGGTTTGAG CTACAGTTCC GATTGGGCCC AACTTTGCAG 
GGAAAACCAG TTACTGTATA TACAAATTAT CCATTTCCTG GAGAAACATT TAATCGACAT 
AAATTCCGTT CCCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAGCA GTCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAGAGTGGTG GAGGTTACAT AGTCGTGGAT CCTATTTTAC GAGTTGGTGC TGATAGCCAT 
GTGTTACCCT TGGATTGTGT TACTCTGCAG ACATTTTTAG CTAAGTGTTT AGGACCTTTT 
GATGAATGGG AAAGCAGAAT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCACTTC 
ACCCCGCTGC AGACTCTTGG ACTGTCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGATAGA AAGTACACCT GGCATGATGT TGGGCAACTG 
GTAGAAAAAC TGAAGAAGGA GTGGAATGTT CTTTGTATTA CAGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GCTCCAGGAA CACCCAGAGT CTGCATATAA TCTTGTGAAT 
TCTCCACATT TAAAACCGGC CTGGATTTTA GACAGAGCAC TTTGGCATTT CTCCTGTGAG 
GTTGCAGAAG GGAAATATAG AGAAAAAGGA ATCCCTGCTT TGATTGAAAA TGATCACCAC 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATGTTTACC CAAAGATTAA ACTGTGGGAA 
TTTTTCCAAG TACATGAACA CAGAGCAGTT GAGGAATTTA GAAGGCTGCT CACACAAGAA 
AATAGAAGAA CAACCAAGTC TGATCCAAAA CAACATCTTA AGATTATTCA GGATCCTGAA 
TATAGACGCC TTGGCTGTAC TATAGATATG AACATTGCAT TGGCAACTTT CATACCACAT 
AACAATGGAC CAGCCGCAAT TGAAGAATGC TGTAATTGGT TTCGTAAGAA AATTGAGGAA 
TTAAATTCAG AGAAATATCA ACTCATACAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGAATGGCT GGCCATGGTC CTAAACTAGG ACCTGTCACT 
AGAAAACATC CCTTAGTTAC CAGGTATTTT ACTTTCCCAT TCCAAGAAAT GGACTTATCC 
ACAGAAGAGT CCATGATGCA CCTCCCTGAT AAAGCTTGTT TCTTCATGGC ACATAATGGA 
TGGGTGATGG GAGATGACCC TCTTCGAAAC TTTGCTGAGC CAGGTTCAGA TGTTTACTTA 
AGGCGAGAAC TTATATGCTG GGGCGACAGT GTTAAATTGC GCTACGGAAA TAAGCCAGAG 
GATTGTCCTT ACCTATGGGC ACACATGAAA AAATACACTG AAATCACTGC GACTTATTTC 
CAGGGTGTAC GTCTTGATAA CTGCCACTCA ACACCTATTC ATGTGGCTGA GTACATGTTG 
GATGCTGCTA GGAAATTACA ACCCAATTTA TATGTAGTAG CTGAACTATT CACAGGAAGT 
GAAGAGCTGG ACAATATCTT TGTCACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCG 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG TGGAGAACCG 
GTTGGATCCT TTGTTCAGCC CTGTCTGAGG CCTTTAATGC CAGCTATCGC ACATGCCCTG 
TTTATGGATA TTACCCATGA TAACGAGTGT CCTATTGTGC ATAGATCAGC CTACGATGCT 
CTTCCAAGTA CCACAATTGT GTCTATGGCA TCTTGTGCTA GTGGAAGTAC ACGAGGCTAT 
GATGAATTAG TGCCTCATCA GATATCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGGAG CATTGCCATC AAATGTGGGT GATGTTAATT TCCAGAGTGG AATTATTGCA 
GCCAGATGTG CTATCAATAA ACTTCATCAA GAGCTCGGAG CCAAAGGTTT TATTCAGGTA 
TATGTAGATC AAGTTGATGA AGACATAGTG GCAGTGACCA GACATTCACC TAGTATCCAT 
CAGTCCGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAAACTTC GTTTTACAGC 
AAGGAAGTAC CTCAGATGTG CATCCCTGGC AAAATTGAAG AGGTTGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAACACACA ACCATATGTA AAGGATGAGC ATTCAATCAA CGGGCTGCCA 
AACATCACAG TAGAAATTAG GGAACATATT CAGCTCAAAG AAAGTAAAAT TGTAAAACAA 
GCTGGAATTG CCACAAAAGG TCCAAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATCGT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GGAACCATCT GACACAGTTC AGTCCGCACT TTAAATCTGG AAGCCTTGCT 
GTTGACAACT CGGATCCTAT TTTGAAAATC CCATTTGCTT CTATTGCCTC TAACTTGACT 
TTGGCTGAGC TAAATCAAAT ACTTTACCGA TGTGAATCAG AGGAACAAGA AGATGGTGGA 
GGTTGTTACA ACATACCAAA CTGGTCATCC CTTAAATACG CAGGTCTTCA AGGATTAATG 
TCTGTATTGG CAGAAATTAG ACCAAAGAAT GATTTGGGGC ATCCTTTTTG TGATAATTTG 
AGATCCGGAG ATTGGATGAT TGACTATGTC AGTGGCCGGC TTATTTCACG ATCAGGAAAT 
ATTGCTGAAG TCGGCAAATG GTTACAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGA 
TATCTTATCC CATGTTATTT TGATGCAATA TTAATTGGTG TATATACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACATTTGT AAAACACCTT 
TCATTGGGGT CAGTTCAGAT GTGTGGAGTA GGAAAATTCC CTTGTCTGCC CCTTCTGTCT 
CCTTCACTTT GGGATGTGCC ACACAGGGTA AATGAGATCA CAAAAGAAAA GGAGCAGTGT 
TGTGCTTCTC TGGCTGCAGG CTTACCTCAT TTTTCTGCTG GTCTTTTCCG CTGTTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTCTT TTGCTGGTTA CTGGACGCTA CTTAGAGGCC 
AGGAACATTA TTTTAGCATT CGCTGGTACT CTGAGGCATG GTCTTATCCC CAACCTCCTT 
GGTGAAGGAG TTTATGCCAG GTACAATTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGATT ACTGTAAGAT CGTCCCGAAC GGTCTAGACA TTCTCAAGTG TCCAGTGTCC 
AGGATGTATC CAACAGATGA TTCTGCTCCT TTGCCTGCTG GTACACTGGA TCAACCACTA 
TTTGAAGTGA TACAAGAAAC TATGCAAAGA CATATGCAGG GCATACAGTT CCGAGAGAGA 
AATGCTGGCT CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT AACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGT GGAAATCGCT TCAATTGTGG CACGTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TGGAAATTGT AGGCCTTGCC AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTTCC TTATCATGAA GTTAGAATAA AAAGACACGG GAAAGTTGTA 
GCCGTCTCAT ATGATGAGTG GAACAGGAAA ATACAAGACA ACTTTGAAAA GCTGTTTCAT 
GTTTCGGAAG ACCCTTCAGA CCCTAATGAG AAGCATCCAA ATCTAGTCCA CAAACGGGGC 
ATCTACAAAG ATAGTTACGG AGCTTCCAGT CCTTGGTGTG ACTACCAGCT CAGGCCGAAC 
TTTACCATCG CAATGGTCGT GGCCCCTGAG CTCTTTACTG CAGGAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTGGGC CCCCTAGGCA TGAAAACCTT AGATCCAGAT 
GATATGGTGT ACTGTGGAAT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AGAGGTTTCA ATTATCATCA AGGACCTGAG TGGCTATGGC CTGTTGGATA TTTCCTTCGT 
GCAAAGTTAT ATTTTGCCAA ATTGATGGGT CCAGAGACTA CTGCAAAAAC TGTGTTTTTG 
GTCAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCTATTG GAAAGGTCTT 
CCAGAACTGA CCAATGAAAA TGGCCAATAC TGTCCTTTCA GCTGTGAGAC ACAAGCTTGG 
TCAATTGCTA CCGTTCTTGA AACACTTTAT GATTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agl ( XM_013142992.1 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_012998446.1 Cavia porcellus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X3, mRNA.

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CloneID	ODo24114
Clone ID Related Accession (Same CDS sequence)	XM_013142992.2 , XM_013142991.2 , XM_013142992.1 , XM_003479171.3 , XM_003479171.4 , XM_013142991.1
Accession Version	XM_013142992.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436716800000
Organism	Cavia porcellus(domestic guinea pig)
Product	glycogen debranching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176417.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cavia porcellus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACACA GTAAACAGAT TCGAATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTTTTCA GACTTGAGCA AGGGTTTGAG CTACAGTTCC GATTGGGCCC AACTTTGCAG 
GGAAAACCAG TTACTGTATA TACAAATTAT CCATTTCCTG GAGAAACATT TAATCGACAT 
AAATTCCGTT CCCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAGCA GTCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAGAGTGGTG GAGGTTACAT AGTCGTGGAT CCTATTTTAC GAGTTGGTGC TGATAGCCAT 
GTGTTACCCT TGGATTGTGT TACTCTGCAG ACATTTTTAG CTAAGTGTTT AGGACCTTTT 
GATGAATGGG AAAGCAGAAT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCACTTC 
ACCCCGCTGC AGACTCTTGG ACTGTCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGATAGA AAGTACACCT GGCATGATGT TGGGCAACTG 
GTAGAAAAAC TGAAGAAGGA GTGGAATGTT CTTTGTATTA CAGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GCTCCAGGAA CACCCAGAGT CTGCATATAA TCTTGTGAAT 
TCTCCACATT TAAAACCGGC CTGGATTTTA GACAGAGCAC TTTGGCATTT CTCCTGTGAG 
GTTGCAGAAG GGAAATATAG AGAAAAAGGA ATCCCTGCTT TGATTGAAAA TGATCACCAC 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATGTTTACC CAAAGATTAA ACTGTGGGAA 
TTTTTCCAAG TACATGAACA CAGAGCAGTT GAGGAATTTA GAAGGCTGCT CACACAAGAA 
AATAGAAGAA CAACCAAGTC TGATCCAAAA CAACATCTTA AGATTATTCA GGATCCTGAA 
TATAGACGCC TTGGCTGTAC TATAGATATG AACATTGCAT TGGCAACTTT CATACCACAT 
AACAATGGAC CAGCCGCAAT TGAAGAATGC TGTAATTGGT TTCGTAAGAA AATTGAGGAA 
TTAAATTCAG AGAAATATCA ACTCATACAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGAATGGCT GGCCATGGTC CTAAACTAGG ACCTGTCACT 
AGAAAACATC CCTTAGTTAC CAGGTATTTT ACTTTCCCAT TCCAAGAAAT GGACTTATCC 
ACAGAAGAGT CCATGATGCA CCTCCCTGAT AAAGCTTGTT TCTTCATGGC ACATAATGGA 
TGGGTGATGG GAGATGACCC TCTTCGAAAC TTTGCTGAGC CAGGTTCAGA TGTTTACTTA 
AGGCGAGAAC TTATATGCTG GGGCGACAGT GTTAAATTGC GCTACGGAAA TAAGCCAGAG 
GATTGTCCTT ACCTATGGGC ACACATGAAA AAATACACTG AAATCACTGC GACTTATTTC 
CAGGGTGTAC GTCTTGATAA CTGCCACTCA ACACCTATTC ATGTGGCTGA GTACATGTTG 
GATGCTGCTA GGAAATTACA ACCCAATTTA TATGTAGTAG CTGAACTATT CACAGGAAGT 
GAAGAGCTGG ACAATATCTT TGTCACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCG 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG TGGAGAACCG 
GTTGGATCCT TTGTTCAGCC CTGTCTGAGG CCTTTAATGC CAGCTATCGC ACATGCCCTG 
TTTATGGATA TTACCCATGA TAACGAGTGT CCTATTGTGC ATAGATCAGC CTACGATGCT 
CTTCCAAGTA CCACAATTGT GTCTATGGCA TCTTGTGCTA GTGGAAGTAC ACGAGGCTAT 
GATGAATTAG TGCCTCATCA GATATCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGGAG CATTGCCATC AAATGTGGGT GATGTTAATT TCCAGAGTGG AATTATTGCA 
GCCAGATGTG CTATCAATAA ACTTCATCAA GAGCTCGGAG CCAAAGGTTT TATTCAGGTA 
TATGTAGATC AAGTTGATGA AGACATAGTG GCAGTGACCA GACATTCACC TAGTATCCAT 
CAGTCCGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAAACTTC GTTTTACAGC 
AAGGAAGTAC CTCAGATGTG CATCCCTGGC AAAATTGAAG AGGTTGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAACACACA ACCATATGTA AAGGATGAGC ATTCAATCAA CGGGCTGCCA 
AACATCACAG TAGAAATTAG GGAACATATT CAGCTCAAAG AAAGTAAAAT TGTAAAACAA 
GCTGGAATTG CCACAAAAGG TCCAAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATCGT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GGAACCATCT GACACAGTTC AGTCCGCACT TTAAATCTGG AAGCCTTGCT 
GTTGACAACT CGGATCCTAT TTTGAAAATC CCATTTGCTT CTATTGCCTC TAACTTGACT 
TTGGCTGAGC TAAATCAAAT ACTTTACCGA TGTGAATCAG AGGAACAAGA AGATGGTGGA 
GGTTGTTACA ACATACCAAA CTGGTCATCC CTTAAATACG CAGGTCTTCA AGGATTAATG 
TCTGTATTGG CAGAAATTAG ACCAAAGAAT GATTTGGGGC ATCCTTTTTG TGATAATTTG 
AGATCCGGAG ATTGGATGAT TGACTATGTC AGTGGCCGGC TTATTTCACG ATCAGGAAAT 
ATTGCTGAAG TCGGCAAATG GTTACAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGA 
TATCTTATCC CATGTTATTT TGATGCAATA TTAATTGGTG TATATACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACATTTGT AAAACACCTT 
TCATTGGGGT CAGTTCAGAT GTGTGGAGTA GGAAAATTCC CTTGTCTGCC CCTTCTGTCT 
CCTTCACTTT GGGATGTGCC ACACAGGGTA AATGAGATCA CAAAAGAAAA GGAGCAGTGT 
TGTGCTTCTC TGGCTGCAGG CTTACCTCAT TTTTCTGCTG GTCTTTTCCG CTGTTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTCTT TTGCTGGTTA CTGGACGCTA CTTAGAGGCC 
AGGAACATTA TTTTAGCATT CGCTGGTACT CTGAGGCATG GTCTTATCCC CAACCTCCTT 
GGTGAAGGAG TTTATGCCAG GTACAATTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGATT ACTGTAAGAT CGTCCCGAAC GGTCTAGACA TTCTCAAGTG TCCAGTGTCC 
AGGATGTATC CAACAGATGA TTCTGCTCCT TTGCCTGCTG GTACACTGGA TCAACCACTA 
TTTGAAGTGA TACAAGAAAC TATGCAAAGA CATATGCAGG GCATACAGTT CCGAGAGAGA 
AATGCTGGCT CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT AACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGT GGAAATCGCT TCAATTGTGG CACGTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TGGAAATTGT AGGCCTTGCC AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTTCC TTATCATGAA GTTAGAATAA AAAGACACGG GAAAGTTGTA 
GCCGTCTCAT ATGATGAGTG GAACAGGAAA ATACAAGACA ACTTTGAAAA GCTGTTTCAT 
GTTTCGGAAG ACCCTTCAGA CCCTAATGAG AAGCATCCAA ATCTAGTCCA CAAACGGGGC 
ATCTACAAAG ATAGTTACGG AGCTTCCAGT CCTTGGTGTG ACTACCAGCT CAGGCCGAAC 
TTTACCATCG CAATGGTCGT GGCCCCTGAG CTCTTTACTG CAGGAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTGGGC CCCCTAGGCA TGAAAACCTT AGATCCAGAT 
GATATGGTGT ACTGTGGAAT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AGAGGTTTCA ATTATCATCA AGGACCTGAG TGGCTATGGC CTGTTGGATA TTTCCTTCGT 
GCAAAGTTAT ATTTTGCCAA ATTGATGGGT CCAGAGACTA CTGCAAAAAC TGTGTTTTTG 
GTCAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCTATTG GAAAGGTCTT 
CCAGAACTGA CCAATGAAAA TGGCCAATAC TGTCCTTTCA GCTGTGAGAC ACAAGCTTGG 
TCAATTGCTA CCGTTCTTGA AACACTTTAT GATTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012998446.1
CDS	180..4778
Translation	MEHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKPVTVYTNYPFPGETFNRHKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADSHVLPLDCVTLQTFLAKCLGPFDEWESRIRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPDRKYTWHDVGQLVEKLKKEWNVLCITDVVYNHTAANSKWLQEHPESAYNLVNSPHLKPAWILDRALWHFSCEVAEGKYREKGIPALIENDHHMNCIRKVIWEDVYPKIKLWEFFQVHEHRAVEEFRRLLTQENRRTTKSDPKQHLKIIQDPEYRRLGCTIDMNIALATFIPHNNGPAAIEECCNWFRKKIEELNSEKYQLIHYHQEQAVNCLLGNVFYERMAGHGPKLGPVTRKHPLVTRYFTFPFQEMDLSTEESMMHLPDKACFFMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPIHVAEYMLDAARKLQPNLYVVAELFTGSEELDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMASCASGSTRGYDELVPHQISVVSEERFYTKWNPGALPSNVGDVNFQSGIIAARCAINKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTVERNTQPYVKDEHSINGLPNITVEIREHIQLKESKIVKQAGIATKGPNEYIQEIEFENLSPGSVIVFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNSDPILKIPFASIASNLTLAELNQILYRCESEEQEDGGGCYNIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSGRLISRSGNIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGVYTTLLDIAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGKFPCLPLLSPSLWDVPHRVNEITKEKEQCCASLAAGLPHFSAGLFRCWGRDTFIALRGLLLVTGRYLEARNIILAFAGTLRHGLIPNLLGEGVYARYNCRDAVWWWLQCIQDYCKIVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQETMQRHMQGIQFRERNAGSQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLAKSAVRWLLELSKKNIFPYHEVRIKRHGKVVAVSYDEWNRKIQDNFEKLFHVSEDPSDPNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAGKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLARGFNYHQGPEWLWPVGYFLRAKLYFAKLMGPETTAKTVFLVKNVLSRHYVHLERSYWKGLPELTNENGQYCPFSCETQAWSIATVLETLYDL

Target ORF information:

RefSeq Version	XM_013142992.1
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013142992.1

ORF Insert Sequence:

ATGGAACACA GTAAACAGAT TCGAATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTTTTCA GACTTGAGCA AGGGTTTGAG CTACAGTTCC GATTGGGCCC AACTTTGCAG 
GGAAAACCAG TTACTGTATA TACAAATTAT CCATTTCCTG GAGAAACATT TAATCGACAT 
AAATTCCGTT CCCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAGCA GTCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAGAGTGGTG GAGGTTACAT AGTCGTGGAT CCTATTTTAC GAGTTGGTGC TGATAGCCAT 
GTGTTACCCT TGGATTGTGT TACTCTGCAG ACATTTTTAG CTAAGTGTTT AGGACCTTTT 
GATGAATGGG AAAGCAGAAT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCACTTC 
ACCCCGCTGC AGACTCTTGG ACTGTCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGATAGA AAGTACACCT GGCATGATGT TGGGCAACTG 
GTAGAAAAAC TGAAGAAGGA GTGGAATGTT CTTTGTATTA CAGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GCTCCAGGAA CACCCAGAGT CTGCATATAA TCTTGTGAAT 
TCTCCACATT TAAAACCGGC CTGGATTTTA GACAGAGCAC TTTGGCATTT CTCCTGTGAG 
GTTGCAGAAG GGAAATATAG AGAAAAAGGA ATCCCTGCTT TGATTGAAAA TGATCACCAC 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATGTTTACC CAAAGATTAA ACTGTGGGAA 
TTTTTCCAAG TACATGAACA CAGAGCAGTT GAGGAATTTA GAAGGCTGCT CACACAAGAA 
AATAGAAGAA CAACCAAGTC TGATCCAAAA CAACATCTTA AGATTATTCA GGATCCTGAA 
TATAGACGCC TTGGCTGTAC TATAGATATG AACATTGCAT TGGCAACTTT CATACCACAT 
AACAATGGAC CAGCCGCAAT TGAAGAATGC TGTAATTGGT TTCGTAAGAA AATTGAGGAA 
TTAAATTCAG AGAAATATCA ACTCATACAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGAATGGCT GGCCATGGTC CTAAACTAGG ACCTGTCACT 
AGAAAACATC CCTTAGTTAC CAGGTATTTT ACTTTCCCAT TCCAAGAAAT GGACTTATCC 
ACAGAAGAGT CCATGATGCA CCTCCCTGAT AAAGCTTGTT TCTTCATGGC ACATAATGGA 
TGGGTGATGG GAGATGACCC TCTTCGAAAC TTTGCTGAGC CAGGTTCAGA TGTTTACTTA 
AGGCGAGAAC TTATATGCTG GGGCGACAGT GTTAAATTGC GCTACGGAAA TAAGCCAGAG 
GATTGTCCTT ACCTATGGGC ACACATGAAA AAATACACTG AAATCACTGC GACTTATTTC 
CAGGGTGTAC GTCTTGATAA CTGCCACTCA ACACCTATTC ATGTGGCTGA GTACATGTTG 
GATGCTGCTA GGAAATTACA ACCCAATTTA TATGTAGTAG CTGAACTATT CACAGGAAGT 
GAAGAGCTGG ACAATATCTT TGTCACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCG 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG TGGAGAACCG 
GTTGGATCCT TTGTTCAGCC CTGTCTGAGG CCTTTAATGC CAGCTATCGC ACATGCCCTG 
TTTATGGATA TTACCCATGA TAACGAGTGT CCTATTGTGC ATAGATCAGC CTACGATGCT 
CTTCCAAGTA CCACAATTGT GTCTATGGCA TCTTGTGCTA GTGGAAGTAC ACGAGGCTAT 
GATGAATTAG TGCCTCATCA GATATCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGGAG CATTGCCATC AAATGTGGGT GATGTTAATT TCCAGAGTGG AATTATTGCA 
GCCAGATGTG CTATCAATAA ACTTCATCAA GAGCTCGGAG CCAAAGGTTT TATTCAGGTA 
TATGTAGATC AAGTTGATGA AGACATAGTG GCAGTGACCA GACATTCACC TAGTATCCAT 
CAGTCCGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAAACTTC GTTTTACAGC 
AAGGAAGTAC CTCAGATGTG CATCCCTGGC AAAATTGAAG AGGTTGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAACACACA ACCATATGTA AAGGATGAGC ATTCAATCAA CGGGCTGCCA 
AACATCACAG TAGAAATTAG GGAACATATT CAGCTCAAAG AAAGTAAAAT TGTAAAACAA 
GCTGGAATTG CCACAAAAGG TCCAAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATCGT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GGAACCATCT GACACAGTTC AGTCCGCACT TTAAATCTGG AAGCCTTGCT 
GTTGACAACT CGGATCCTAT TTTGAAAATC CCATTTGCTT CTATTGCCTC TAACTTGACT 
TTGGCTGAGC TAAATCAAAT ACTTTACCGA TGTGAATCAG AGGAACAAGA AGATGGTGGA 
GGTTGTTACA ACATACCAAA CTGGTCATCC CTTAAATACG CAGGTCTTCA AGGATTAATG 
TCTGTATTGG CAGAAATTAG ACCAAAGAAT GATTTGGGGC ATCCTTTTTG TGATAATTTG 
AGATCCGGAG ATTGGATGAT TGACTATGTC AGTGGCCGGC TTATTTCACG ATCAGGAAAT 
ATTGCTGAAG TCGGCAAATG GTTACAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGA 
TATCTTATCC CATGTTATTT TGATGCAATA TTAATTGGTG TATATACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACATTTGT AAAACACCTT 
TCATTGGGGT CAGTTCAGAT GTGTGGAGTA GGAAAATTCC CTTGTCTGCC CCTTCTGTCT 
CCTTCACTTT GGGATGTGCC ACACAGGGTA AATGAGATCA CAAAAGAAAA GGAGCAGTGT 
TGTGCTTCTC TGGCTGCAGG CTTACCTCAT TTTTCTGCTG GTCTTTTCCG CTGTTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTCTT TTGCTGGTTA CTGGACGCTA CTTAGAGGCC 
AGGAACATTA TTTTAGCATT CGCTGGTACT CTGAGGCATG GTCTTATCCC CAACCTCCTT 
GGTGAAGGAG TTTATGCCAG GTACAATTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGATT ACTGTAAGAT CGTCCCGAAC GGTCTAGACA TTCTCAAGTG TCCAGTGTCC 
AGGATGTATC CAACAGATGA TTCTGCTCCT TTGCCTGCTG GTACACTGGA TCAACCACTA 
TTTGAAGTGA TACAAGAAAC TATGCAAAGA CATATGCAGG GCATACAGTT CCGAGAGAGA 
AATGCTGGCT CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT AACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGT GGAAATCGCT TCAATTGTGG CACGTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TGGAAATTGT AGGCCTTGCC AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTTCC TTATCATGAA GTTAGAATAA AAAGACACGG GAAAGTTGTA 
GCCGTCTCAT ATGATGAGTG GAACAGGAAA ATACAAGACA ACTTTGAAAA GCTGTTTCAT 
GTTTCGGAAG ACCCTTCAGA CCCTAATGAG AAGCATCCAA ATCTAGTCCA CAAACGGGGC 
ATCTACAAAG ATAGTTACGG AGCTTCCAGT CCTTGGTGTG ACTACCAGCT CAGGCCGAAC 
TTTACCATCG CAATGGTCGT GGCCCCTGAG CTCTTTACTG CAGGAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTGGGC CCCCTAGGCA TGAAAACCTT AGATCCAGAT 
GATATGGTGT ACTGTGGAAT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AGAGGTTTCA ATTATCATCA AGGACCTGAG TGGCTATGGC CTGTTGGATA TTTCCTTCGT 
GCAAAGTTAT ATTTTGCCAA ATTGATGGGT CCAGAGACTA CTGCAAAAAC TGTGTTTTTG 
GTCAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCTATTG GAAAGGTCTT 
CCAGAACTGA CCAATGAAAA TGGCCAATAC TGTCCTTTCA GCTGTGAGAC ACAAGCTTGG 
TCAATTGCTA CCGTTCTTGA AACACTTTAT GATTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agl ( XM_003479171.3 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_003479219.1 Cavia porcellus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X2, mRNA.

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CloneID	ODo24114
Clone ID Related Accession (Same CDS sequence)	XM_013142992.2 , XM_013142991.2 , XM_013142992.1 , XM_003479171.3 , XM_003479171.4 , XM_013142991.1
Accession Version	XM_003479171.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436716800000
Organism	Cavia porcellus(domestic guinea pig)
Product	glycogen debranching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176417.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 14, 2015 this sequence version replaced XM_003479171.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cavia porcellus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACACA GTAAACAGAT TCGAATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTTTTCA GACTTGAGCA AGGGTTTGAG CTACAGTTCC GATTGGGCCC AACTTTGCAG 
GGAAAACCAG TTACTGTATA TACAAATTAT CCATTTCCTG GAGAAACATT TAATCGACAT 
AAATTCCGTT CCCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAGCA GTCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAGAGTGGTG GAGGTTACAT AGTCGTGGAT CCTATTTTAC GAGTTGGTGC TGATAGCCAT 
GTGTTACCCT TGGATTGTGT TACTCTGCAG ACATTTTTAG CTAAGTGTTT AGGACCTTTT 
GATGAATGGG AAAGCAGAAT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCACTTC 
ACCCCGCTGC AGACTCTTGG ACTGTCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGATAGA AAGTACACCT GGCATGATGT TGGGCAACTG 
GTAGAAAAAC TGAAGAAGGA GTGGAATGTT CTTTGTATTA CAGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GCTCCAGGAA CACCCAGAGT CTGCATATAA TCTTGTGAAT 
TCTCCACATT TAAAACCGGC CTGGATTTTA GACAGAGCAC TTTGGCATTT CTCCTGTGAG 
GTTGCAGAAG GGAAATATAG AGAAAAAGGA ATCCCTGCTT TGATTGAAAA TGATCACCAC 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATGTTTACC CAAAGATTAA ACTGTGGGAA 
TTTTTCCAAG TACATGAACA CAGAGCAGTT GAGGAATTTA GAAGGCTGCT CACACAAGAA 
AATAGAAGAA CAACCAAGTC TGATCCAAAA CAACATCTTA AGATTATTCA GGATCCTGAA 
TATAGACGCC TTGGCTGTAC TATAGATATG AACATTGCAT TGGCAACTTT CATACCACAT 
AACAATGGAC CAGCCGCAAT TGAAGAATGC TGTAATTGGT TTCGTAAGAA AATTGAGGAA 
TTAAATTCAG AGAAATATCA ACTCATACAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGAATGGCT GGCCATGGTC CTAAACTAGG ACCTGTCACT 
AGAAAACATC CCTTAGTTAC CAGGTATTTT ACTTTCCCAT TCCAAGAAAT GGACTTATCC 
ACAGAAGAGT CCATGATGCA CCTCCCTGAT AAAGCTTGTT TCTTCATGGC ACATAATGGA 
TGGGTGATGG GAGATGACCC TCTTCGAAAC TTTGCTGAGC CAGGTTCAGA TGTTTACTTA 
AGGCGAGAAC TTATATGCTG GGGCGACAGT GTTAAATTGC GCTACGGAAA TAAGCCAGAG 
GATTGTCCTT ACCTATGGGC ACACATGAAA AAATACACTG AAATCACTGC GACTTATTTC 
CAGGGTGTAC GTCTTGATAA CTGCCACTCA ACACCTATTC ATGTGGCTGA GTACATGTTG 
GATGCTGCTA GGAAATTACA ACCCAATTTA TATGTAGTAG CTGAACTATT CACAGGAAGT 
GAAGAGCTGG ACAATATCTT TGTCACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCG 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG TGGAGAACCG 
GTTGGATCCT TTGTTCAGCC CTGTCTGAGG CCTTTAATGC CAGCTATCGC ACATGCCCTG 
TTTATGGATA TTACCCATGA TAACGAGTGT CCTATTGTGC ATAGATCAGC CTACGATGCT 
CTTCCAAGTA CCACAATTGT GTCTATGGCA TCTTGTGCTA GTGGAAGTAC ACGAGGCTAT 
GATGAATTAG TGCCTCATCA GATATCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGGAG CATTGCCATC AAATGTGGGT GATGTTAATT TCCAGAGTGG AATTATTGCA 
GCCAGATGTG CTATCAATAA ACTTCATCAA GAGCTCGGAG CCAAAGGTTT TATTCAGGTA 
TATGTAGATC AAGTTGATGA AGACATAGTG GCAGTGACCA GACATTCACC TAGTATCCAT 
CAGTCCGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAAACTTC GTTTTACAGC 
AAGGAAGTAC CTCAGATGTG CATCCCTGGC AAAATTGAAG AGGTTGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAACACACA ACCATATGTA AAGGATGAGC ATTCAATCAA CGGGCTGCCA 
AACATCACAG TAGAAATTAG GGAACATATT CAGCTCAAAG AAAGTAAAAT TGTAAAACAA 
GCTGGAATTG CCACAAAAGG TCCAAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATCGT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GGAACCATCT GACACAGTTC AGTCCGCACT TTAAATCTGG AAGCCTTGCT 
GTTGACAACT CGGATCCTAT TTTGAAAATC CCATTTGCTT CTATTGCCTC TAACTTGACT 
TTGGCTGAGC TAAATCAAAT ACTTTACCGA TGTGAATCAG AGGAACAAGA AGATGGTGGA 
GGTTGTTACA ACATACCAAA CTGGTCATCC CTTAAATACG CAGGTCTTCA AGGATTAATG 
TCTGTATTGG CAGAAATTAG ACCAAAGAAT GATTTGGGGC ATCCTTTTTG TGATAATTTG 
AGATCCGGAG ATTGGATGAT TGACTATGTC AGTGGCCGGC TTATTTCACG ATCAGGAAAT 
ATTGCTGAAG TCGGCAAATG GTTACAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGA 
TATCTTATCC CATGTTATTT TGATGCAATA TTAATTGGTG TATATACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACATTTGT AAAACACCTT 
TCATTGGGGT CAGTTCAGAT GTGTGGAGTA GGAAAATTCC CTTGTCTGCC CCTTCTGTCT 
CCTTCACTTT GGGATGTGCC ACACAGGGTA AATGAGATCA CAAAAGAAAA GGAGCAGTGT 
TGTGCTTCTC TGGCTGCAGG CTTACCTCAT TTTTCTGCTG GTCTTTTCCG CTGTTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTCTT TTGCTGGTTA CTGGACGCTA CTTAGAGGCC 
AGGAACATTA TTTTAGCATT CGCTGGTACT CTGAGGCATG GTCTTATCCC CAACCTCCTT 
GGTGAAGGAG TTTATGCCAG GTACAATTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGATT ACTGTAAGAT CGTCCCGAAC GGTCTAGACA TTCTCAAGTG TCCAGTGTCC 
AGGATGTATC CAACAGATGA TTCTGCTCCT TTGCCTGCTG GTACACTGGA TCAACCACTA 
TTTGAAGTGA TACAAGAAAC TATGCAAAGA CATATGCAGG GCATACAGTT CCGAGAGAGA 
AATGCTGGCT CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT AACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGT GGAAATCGCT TCAATTGTGG CACGTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TGGAAATTGT AGGCCTTGCC AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTTCC TTATCATGAA GTTAGAATAA AAAGACACGG GAAAGTTGTA 
GCCGTCTCAT ATGATGAGTG GAACAGGAAA ATACAAGACA ACTTTGAAAA GCTGTTTCAT 
GTTTCGGAAG ACCCTTCAGA CCCTAATGAG AAGCATCCAA ATCTAGTCCA CAAACGGGGC 
ATCTACAAAG ATAGTTACGG AGCTTCCAGT CCTTGGTGTG ACTACCAGCT CAGGCCGAAC 
TTTACCATCG CAATGGTCGT GGCCCCTGAG CTCTTTACTG CAGGAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTGGGC CCCCTAGGCA TGAAAACCTT AGATCCAGAT 
GATATGGTGT ACTGTGGAAT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AGAGGTTTCA ATTATCATCA AGGACCTGAG TGGCTATGGC CTGTTGGATA TTTCCTTCGT 
GCAAAGTTAT ATTTTGCCAA ATTGATGGGT CCAGAGACTA CTGCAAAAAC TGTGTTTTTG 
GTCAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCTATTG GAAAGGTCTT 
CCAGAACTGA CCAATGAAAA TGGCCAATAC TGTCCTTTCA GCTGTGAGAC ACAAGCTTGG 
TCAATTGCTA CCGTTCTTGA AACACTTTAT GATTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003479219.1
CDS	158..4756
Translation	MEHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKPVTVYTNYPFPGETFNRHKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADSHVLPLDCVTLQTFLAKCLGPFDEWESRIRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPDRKYTWHDVGQLVEKLKKEWNVLCITDVVYNHTAANSKWLQEHPESAYNLVNSPHLKPAWILDRALWHFSCEVAEGKYREKGIPALIENDHHMNCIRKVIWEDVYPKIKLWEFFQVHEHRAVEEFRRLLTQENRRTTKSDPKQHLKIIQDPEYRRLGCTIDMNIALATFIPHNNGPAAIEECCNWFRKKIEELNSEKYQLIHYHQEQAVNCLLGNVFYERMAGHGPKLGPVTRKHPLVTRYFTFPFQEMDLSTEESMMHLPDKACFFMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPIHVAEYMLDAARKLQPNLYVVAELFTGSEELDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMASCASGSTRGYDELVPHQISVVSEERFYTKWNPGALPSNVGDVNFQSGIIAARCAINKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTVERNTQPYVKDEHSINGLPNITVEIREHIQLKESKIVKQAGIATKGPNEYIQEIEFENLSPGSVIVFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNSDPILKIPFASIASNLTLAELNQILYRCESEEQEDGGGCYNIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSGRLISRSGNIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGVYTTLLDIAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGKFPCLPLLSPSLWDVPHRVNEITKEKEQCCASLAAGLPHFSAGLFRCWGRDTFIALRGLLLVTGRYLEARNIILAFAGTLRHGLIPNLLGEGVYARYNCRDAVWWWLQCIQDYCKIVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQETMQRHMQGIQFRERNAGSQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLAKSAVRWLLELSKKNIFPYHEVRIKRHGKVVAVSYDEWNRKIQDNFEKLFHVSEDPSDPNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAGKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLARGFNYHQGPEWLWPVGYFLRAKLYFAKLMGPETTAKTVFLVKNVLSRHYVHLERSYWKGLPELTNENGQYCPFSCETQAWSIATVLETLYDL

Target ORF information:

RefSeq Version	XM_003479171.3
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003479171.3

ORF Insert Sequence:

ATGGAACACA GTAAACAGAT TCGAATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTTTTCA GACTTGAGCA AGGGTTTGAG CTACAGTTCC GATTGGGCCC AACTTTGCAG 
GGAAAACCAG TTACTGTATA TACAAATTAT CCATTTCCTG GAGAAACATT TAATCGACAT 
AAATTCCGTT CCCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAGCA GTCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAGAGTGGTG GAGGTTACAT AGTCGTGGAT CCTATTTTAC GAGTTGGTGC TGATAGCCAT 
GTGTTACCCT TGGATTGTGT TACTCTGCAG ACATTTTTAG CTAAGTGTTT AGGACCTTTT 
GATGAATGGG AAAGCAGAAT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCACTTC 
ACCCCGCTGC AGACTCTTGG ACTGTCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGATAGA AAGTACACCT GGCATGATGT TGGGCAACTG 
GTAGAAAAAC TGAAGAAGGA GTGGAATGTT CTTTGTATTA CAGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GCTCCAGGAA CACCCAGAGT CTGCATATAA TCTTGTGAAT 
TCTCCACATT TAAAACCGGC CTGGATTTTA GACAGAGCAC TTTGGCATTT CTCCTGTGAG 
GTTGCAGAAG GGAAATATAG AGAAAAAGGA ATCCCTGCTT TGATTGAAAA TGATCACCAC 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATGTTTACC CAAAGATTAA ACTGTGGGAA 
TTTTTCCAAG TACATGAACA CAGAGCAGTT GAGGAATTTA GAAGGCTGCT CACACAAGAA 
AATAGAAGAA CAACCAAGTC TGATCCAAAA CAACATCTTA AGATTATTCA GGATCCTGAA 
TATAGACGCC TTGGCTGTAC TATAGATATG AACATTGCAT TGGCAACTTT CATACCACAT 
AACAATGGAC CAGCCGCAAT TGAAGAATGC TGTAATTGGT TTCGTAAGAA AATTGAGGAA 
TTAAATTCAG AGAAATATCA ACTCATACAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGAATGGCT GGCCATGGTC CTAAACTAGG ACCTGTCACT 
AGAAAACATC CCTTAGTTAC CAGGTATTTT ACTTTCCCAT TCCAAGAAAT GGACTTATCC 
ACAGAAGAGT CCATGATGCA CCTCCCTGAT AAAGCTTGTT TCTTCATGGC ACATAATGGA 
TGGGTGATGG GAGATGACCC TCTTCGAAAC TTTGCTGAGC CAGGTTCAGA TGTTTACTTA 
AGGCGAGAAC TTATATGCTG GGGCGACAGT GTTAAATTGC GCTACGGAAA TAAGCCAGAG 
GATTGTCCTT ACCTATGGGC ACACATGAAA AAATACACTG AAATCACTGC GACTTATTTC 
CAGGGTGTAC GTCTTGATAA CTGCCACTCA ACACCTATTC ATGTGGCTGA GTACATGTTG 
GATGCTGCTA GGAAATTACA ACCCAATTTA TATGTAGTAG CTGAACTATT CACAGGAAGT 
GAAGAGCTGG ACAATATCTT TGTCACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCG 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG TGGAGAACCG 
GTTGGATCCT TTGTTCAGCC CTGTCTGAGG CCTTTAATGC CAGCTATCGC ACATGCCCTG 
TTTATGGATA TTACCCATGA TAACGAGTGT CCTATTGTGC ATAGATCAGC CTACGATGCT 
CTTCCAAGTA CCACAATTGT GTCTATGGCA TCTTGTGCTA GTGGAAGTAC ACGAGGCTAT 
GATGAATTAG TGCCTCATCA GATATCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGGAG CATTGCCATC AAATGTGGGT GATGTTAATT TCCAGAGTGG AATTATTGCA 
GCCAGATGTG CTATCAATAA ACTTCATCAA GAGCTCGGAG CCAAAGGTTT TATTCAGGTA 
TATGTAGATC AAGTTGATGA AGACATAGTG GCAGTGACCA GACATTCACC TAGTATCCAT 
CAGTCCGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAAACTTC GTTTTACAGC 
AAGGAAGTAC CTCAGATGTG CATCCCTGGC AAAATTGAAG AGGTTGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAACACACA ACCATATGTA AAGGATGAGC ATTCAATCAA CGGGCTGCCA 
AACATCACAG TAGAAATTAG GGAACATATT CAGCTCAAAG AAAGTAAAAT TGTAAAACAA 
GCTGGAATTG CCACAAAAGG TCCAAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATCGT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GGAACCATCT GACACAGTTC AGTCCGCACT TTAAATCTGG AAGCCTTGCT 
GTTGACAACT CGGATCCTAT TTTGAAAATC CCATTTGCTT CTATTGCCTC TAACTTGACT 
TTGGCTGAGC TAAATCAAAT ACTTTACCGA TGTGAATCAG AGGAACAAGA AGATGGTGGA 
GGTTGTTACA ACATACCAAA CTGGTCATCC CTTAAATACG CAGGTCTTCA AGGATTAATG 
TCTGTATTGG CAGAAATTAG ACCAAAGAAT GATTTGGGGC ATCCTTTTTG TGATAATTTG 
AGATCCGGAG ATTGGATGAT TGACTATGTC AGTGGCCGGC TTATTTCACG ATCAGGAAAT 
ATTGCTGAAG TCGGCAAATG GTTACAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGA 
TATCTTATCC CATGTTATTT TGATGCAATA TTAATTGGTG TATATACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACATTTGT AAAACACCTT 
TCATTGGGGT CAGTTCAGAT GTGTGGAGTA GGAAAATTCC CTTGTCTGCC CCTTCTGTCT 
CCTTCACTTT GGGATGTGCC ACACAGGGTA AATGAGATCA CAAAAGAAAA GGAGCAGTGT 
TGTGCTTCTC TGGCTGCAGG CTTACCTCAT TTTTCTGCTG GTCTTTTCCG CTGTTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTCTT TTGCTGGTTA CTGGACGCTA CTTAGAGGCC 
AGGAACATTA TTTTAGCATT CGCTGGTACT CTGAGGCATG GTCTTATCCC CAACCTCCTT 
GGTGAAGGAG TTTATGCCAG GTACAATTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGATT ACTGTAAGAT CGTCCCGAAC GGTCTAGACA TTCTCAAGTG TCCAGTGTCC 
AGGATGTATC CAACAGATGA TTCTGCTCCT TTGCCTGCTG GTACACTGGA TCAACCACTA 
TTTGAAGTGA TACAAGAAAC TATGCAAAGA CATATGCAGG GCATACAGTT CCGAGAGAGA 
AATGCTGGCT CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT AACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGT GGAAATCGCT TCAATTGTGG CACGTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TGGAAATTGT AGGCCTTGCC AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTTCC TTATCATGAA GTTAGAATAA AAAGACACGG GAAAGTTGTA 
GCCGTCTCAT ATGATGAGTG GAACAGGAAA ATACAAGACA ACTTTGAAAA GCTGTTTCAT 
GTTTCGGAAG ACCCTTCAGA CCCTAATGAG AAGCATCCAA ATCTAGTCCA CAAACGGGGC 
ATCTACAAAG ATAGTTACGG AGCTTCCAGT CCTTGGTGTG ACTACCAGCT CAGGCCGAAC 
TTTACCATCG CAATGGTCGT GGCCCCTGAG CTCTTTACTG CAGGAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTGGGC CCCCTAGGCA TGAAAACCTT AGATCCAGAT 
GATATGGTGT ACTGTGGAAT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AGAGGTTTCA ATTATCATCA AGGACCTGAG TGGCTATGGC CTGTTGGATA TTTCCTTCGT 
GCAAAGTTAT ATTTTGCCAA ATTGATGGGT CCAGAGACTA CTGCAAAAAC TGTGTTTTTG 
GTCAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCTATTG GAAAGGTCTT 
CCAGAACTGA CCAATGAAAA TGGCCAATAC TGTCCTTTCA GCTGTGAGAC ACAAGCTTGG 
TCAATTGCTA CCGTTCTTGA AACACTTTAT GATTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agl ( XM_003479171.4 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_003479219.1 Cavia porcellus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X1, mRNA.

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CloneID	ODo24114
Clone ID Related Accession (Same CDS sequence)	XM_013142992.2 , XM_013142991.2 , XM_013142992.1 , XM_003479171.3 , XM_003479171.4 , XM_013142991.1
Accession Version	XM_003479171.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516204800000
Organism	Cavia porcellus(domestic guinea pig)
Product	glycogen debranching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176417.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_003479171.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cavia porcellus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACACA GTAAACAGAT TCGAATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTTTTCA GACTTGAGCA AGGGTTTGAG CTACAGTTCC GATTGGGCCC AACTTTGCAG 
GGAAAACCAG TTACTGTATA TACAAATTAT CCATTTCCTG GAGAAACATT TAATCGACAT 
AAATTCCGTT CCCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAGCA GTCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAGAGTGGTG GAGGTTACAT AGTCGTGGAT CCTATTTTAC GAGTTGGTGC TGATAGCCAT 
GTGTTACCCT TGGATTGTGT TACTCTGCAG ACATTTTTAG CTAAGTGTTT AGGACCTTTT 
GATGAATGGG AAAGCAGAAT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCACTTC 
ACCCCGCTGC AGACTCTTGG ACTGTCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGATAGA AAGTACACCT GGCATGATGT TGGGCAACTG 
GTAGAAAAAC TGAAGAAGGA GTGGAATGTT CTTTGTATTA CAGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GCTCCAGGAA CACCCAGAGT CTGCATATAA TCTTGTGAAT 
TCTCCACATT TAAAACCGGC CTGGATTTTA GACAGAGCAC TTTGGCATTT CTCCTGTGAG 
GTTGCAGAAG GGAAATATAG AGAAAAAGGA ATCCCTGCTT TGATTGAAAA TGATCACCAC 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATGTTTACC CAAAGATTAA ACTGTGGGAA 
TTTTTCCAAG TACATGAACA CAGAGCAGTT GAGGAATTTA GAAGGCTGCT CACACAAGAA 
AATAGAAGAA CAACCAAGTC TGATCCAAAA CAACATCTTA AGATTATTCA GGATCCTGAA 
TATAGACGCC TTGGCTGTAC TATAGATATG AACATTGCAT TGGCAACTTT CATACCACAT 
AACAATGGAC CAGCCGCAAT TGAAGAATGC TGTAATTGGT TTCGTAAGAA AATTGAGGAA 
TTAAATTCAG AGAAATATCA ACTCATACAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGAATGGCT GGCCATGGTC CTAAACTAGG ACCTGTCACT 
AGAAAACATC CCTTAGTTAC CAGGTATTTT ACTTTCCCAT TCCAAGAAAT GGACTTATCC 
ACAGAAGAGT CCATGATGCA CCTCCCTGAT AAAGCTTGTT TCTTCATGGC ACATAATGGA 
TGGGTGATGG GAGATGACCC TCTTCGAAAC TTTGCTGAGC CAGGTTCAGA TGTTTACTTA 
AGGCGAGAAC TTATATGCTG GGGCGACAGT GTTAAATTGC GCTACGGAAA TAAGCCAGAG 
GATTGTCCTT ACCTATGGGC ACACATGAAA AAATACACTG AAATCACTGC GACTTATTTC 
CAGGGTGTAC GTCTTGATAA CTGCCACTCA ACACCTATTC ATGTGGCTGA GTACATGTTG 
GATGCTGCTA GGAAATTACA ACCCAATTTA TATGTAGTAG CTGAACTATT CACAGGAAGT 
GAAGAGCTGG ACAATATCTT TGTCACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCG 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG TGGAGAACCG 
GTTGGATCCT TTGTTCAGCC CTGTCTGAGG CCTTTAATGC CAGCTATCGC ACATGCCCTG 
TTTATGGATA TTACCCATGA TAACGAGTGT CCTATTGTGC ATAGATCAGC CTACGATGCT 
CTTCCAAGTA CCACAATTGT GTCTATGGCA TCTTGTGCTA GTGGAAGTAC ACGAGGCTAT 
GATGAATTAG TGCCTCATCA GATATCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGGAG CATTGCCATC AAATGTGGGT GATGTTAATT TCCAGAGTGG AATTATTGCA 
GCCAGATGTG CTATCAATAA ACTTCATCAA GAGCTCGGAG CCAAAGGTTT TATTCAGGTA 
TATGTAGATC AAGTTGATGA AGACATAGTG GCAGTGACCA GACATTCACC TAGTATCCAT 
CAGTCCGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAAACTTC GTTTTACAGC 
AAGGAAGTAC CTCAGATGTG CATCCCTGGC AAAATTGAAG AGGTTGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAACACACA ACCATATGTA AAGGATGAGC ATTCAATCAA CGGGCTGCCA 
AACATCACAG TAGAAATTAG GGAACATATT CAGCTCAAAG AAAGTAAAAT TGTAAAACAA 
GCTGGAATTG CCACAAAAGG TCCAAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATCGT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GGAACCATCT GACACAGTTC AGTCCGCACT TTAAATCTGG AAGCCTTGCT 
GTTGACAACT CGGATCCTAT TTTGAAAATC CCATTTGCTT CTATTGCCTC TAACTTGACT 
TTGGCTGAGC TAAATCAAAT ACTTTACCGA TGTGAATCAG AGGAACAAGA AGATGGTGGA 
GGTTGTTACA ACATACCAAA CTGGTCATCC CTTAAATACG CAGGTCTTCA AGGATTAATG 
TCTGTATTGG CAGAAATTAG ACCAAAGAAT GATTTGGGGC ATCCTTTTTG TGATAATTTG 
AGATCCGGAG ATTGGATGAT TGACTATGTC AGTGGCCGGC TTATTTCACG ATCAGGAAAT 
ATTGCTGAAG TCGGCAAATG GTTACAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGA 
TATCTTATCC CATGTTATTT TGATGCAATA TTAATTGGTG TATATACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACATTTGT AAAACACCTT 
TCATTGGGGT CAGTTCAGAT GTGTGGAGTA GGAAAATTCC CTTGTCTGCC CCTTCTGTCT 
CCTTCACTTT GGGATGTGCC ACACAGGGTA AATGAGATCA CAAAAGAAAA GGAGCAGTGT 
TGTGCTTCTC TGGCTGCAGG CTTACCTCAT TTTTCTGCTG GTCTTTTCCG CTGTTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTCTT TTGCTGGTTA CTGGACGCTA CTTAGAGGCC 
AGGAACATTA TTTTAGCATT CGCTGGTACT CTGAGGCATG GTCTTATCCC CAACCTCCTT 
GGTGAAGGAG TTTATGCCAG GTACAATTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGATT ACTGTAAGAT CGTCCCGAAC GGTCTAGACA TTCTCAAGTG TCCAGTGTCC 
AGGATGTATC CAACAGATGA TTCTGCTCCT TTGCCTGCTG GTACACTGGA TCAACCACTA 
TTTGAAGTGA TACAAGAAAC TATGCAAAGA CATATGCAGG GCATACAGTT CCGAGAGAGA 
AATGCTGGCT CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT AACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGT GGAAATCGCT TCAATTGTGG CACGTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TGGAAATTGT AGGCCTTGCC AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTTCC TTATCATGAA GTTAGAATAA AAAGACACGG GAAAGTTGTA 
GCCGTCTCAT ATGATGAGTG GAACAGGAAA ATACAAGACA ACTTTGAAAA GCTGTTTCAT 
GTTTCGGAAG ACCCTTCAGA CCCTAATGAG AAGCATCCAA ATCTAGTCCA CAAACGGGGC 
ATCTACAAAG ATAGTTACGG AGCTTCCAGT CCTTGGTGTG ACTACCAGCT CAGGCCGAAC 
TTTACCATCG CAATGGTCGT GGCCCCTGAG CTCTTTACTG CAGGAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTGGGC CCCCTAGGCA TGAAAACCTT AGATCCAGAT 
GATATGGTGT ACTGTGGAAT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AGAGGTTTCA ATTATCATCA AGGACCTGAG TGGCTATGGC CTGTTGGATA TTTCCTTCGT 
GCAAAGTTAT ATTTTGCCAA ATTGATGGGT CCAGAGACTA CTGCAAAAAC TGTGTTTTTG 
GTCAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCTATTG GAAAGGTCTT 
CCAGAACTGA CCAATGAAAA TGGCCAATAC TGTCCTTTCA GCTGTGAGAC ACAAGCTTGG 
TCAATTGCTA CCGTTCTTGA AACACTTTAT GATTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003479219.1
CDS	157..4755
Translation	MEHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKPVTVYTNYPFPGETFNRHKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADSHVLPLDCVTLQTFLAKCLGPFDEWESRIRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPDRKYTWHDVGQLVEKLKKEWNVLCITDVVYNHTAANSKWLQEHPESAYNLVNSPHLKPAWILDRALWHFSCEVAEGKYREKGIPALIENDHHMNCIRKVIWEDVYPKIKLWEFFQVHEHRAVEEFRRLLTQENRRTTKSDPKQHLKIIQDPEYRRLGCTIDMNIALATFIPHNNGPAAIEECCNWFRKKIEELNSEKYQLIHYHQEQAVNCLLGNVFYERMAGHGPKLGPVTRKHPLVTRYFTFPFQEMDLSTEESMMHLPDKACFFMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPIHVAEYMLDAARKLQPNLYVVAELFTGSEELDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMASCASGSTRGYDELVPHQISVVSEERFYTKWNPGALPSNVGDVNFQSGIIAARCAINKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTVERNTQPYVKDEHSINGLPNITVEIREHIQLKESKIVKQAGIATKGPNEYIQEIEFENLSPGSVIVFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNSDPILKIPFASIASNLTLAELNQILYRCESEEQEDGGGCYNIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSGRLISRSGNIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGVYTTLLDIAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGKFPCLPLLSPSLWDVPHRVNEITKEKEQCCASLAAGLPHFSAGLFRCWGRDTFIALRGLLLVTGRYLEARNIILAFAGTLRHGLIPNLLGEGVYARYNCRDAVWWWLQCIQDYCKIVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQETMQRHMQGIQFRERNAGSQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLAKSAVRWLLELSKKNIFPYHEVRIKRHGKVVAVSYDEWNRKIQDNFEKLFHVSEDPSDPNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAGKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLARGFNYHQGPEWLWPVGYFLRAKLYFAKLMGPETTAKTVFLVKNVLSRHYVHLERSYWKGLPELTNENGQYCPFSCETQAWSIATVLETLYDL

Target ORF information:

RefSeq Version	XM_003479171.4
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003479171.4

ORF Insert Sequence:

ATGGAACACA GTAAACAGAT TCGAATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTTTTCA GACTTGAGCA AGGGTTTGAG CTACAGTTCC GATTGGGCCC AACTTTGCAG 
GGAAAACCAG TTACTGTATA TACAAATTAT CCATTTCCTG GAGAAACATT TAATCGACAT 
AAATTCCGTT CCCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAGCA GTCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAGAGTGGTG GAGGTTACAT AGTCGTGGAT CCTATTTTAC GAGTTGGTGC TGATAGCCAT 
GTGTTACCCT TGGATTGTGT TACTCTGCAG ACATTTTTAG CTAAGTGTTT AGGACCTTTT 
GATGAATGGG AAAGCAGAAT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCACTTC 
ACCCCGCTGC AGACTCTTGG ACTGTCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGATAGA AAGTACACCT GGCATGATGT TGGGCAACTG 
GTAGAAAAAC TGAAGAAGGA GTGGAATGTT CTTTGTATTA CAGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GCTCCAGGAA CACCCAGAGT CTGCATATAA TCTTGTGAAT 
TCTCCACATT TAAAACCGGC CTGGATTTTA GACAGAGCAC TTTGGCATTT CTCCTGTGAG 
GTTGCAGAAG GGAAATATAG AGAAAAAGGA ATCCCTGCTT TGATTGAAAA TGATCACCAC 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATGTTTACC CAAAGATTAA ACTGTGGGAA 
TTTTTCCAAG TACATGAACA CAGAGCAGTT GAGGAATTTA GAAGGCTGCT CACACAAGAA 
AATAGAAGAA CAACCAAGTC TGATCCAAAA CAACATCTTA AGATTATTCA GGATCCTGAA 
TATAGACGCC TTGGCTGTAC TATAGATATG AACATTGCAT TGGCAACTTT CATACCACAT 
AACAATGGAC CAGCCGCAAT TGAAGAATGC TGTAATTGGT TTCGTAAGAA AATTGAGGAA 
TTAAATTCAG AGAAATATCA ACTCATACAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGAATGGCT GGCCATGGTC CTAAACTAGG ACCTGTCACT 
AGAAAACATC CCTTAGTTAC CAGGTATTTT ACTTTCCCAT TCCAAGAAAT GGACTTATCC 
ACAGAAGAGT CCATGATGCA CCTCCCTGAT AAAGCTTGTT TCTTCATGGC ACATAATGGA 
TGGGTGATGG GAGATGACCC TCTTCGAAAC TTTGCTGAGC CAGGTTCAGA TGTTTACTTA 
AGGCGAGAAC TTATATGCTG GGGCGACAGT GTTAAATTGC GCTACGGAAA TAAGCCAGAG 
GATTGTCCTT ACCTATGGGC ACACATGAAA AAATACACTG AAATCACTGC GACTTATTTC 
CAGGGTGTAC GTCTTGATAA CTGCCACTCA ACACCTATTC ATGTGGCTGA GTACATGTTG 
GATGCTGCTA GGAAATTACA ACCCAATTTA TATGTAGTAG CTGAACTATT CACAGGAAGT 
GAAGAGCTGG ACAATATCTT TGTCACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCG 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG TGGAGAACCG 
GTTGGATCCT TTGTTCAGCC CTGTCTGAGG CCTTTAATGC CAGCTATCGC ACATGCCCTG 
TTTATGGATA TTACCCATGA TAACGAGTGT CCTATTGTGC ATAGATCAGC CTACGATGCT 
CTTCCAAGTA CCACAATTGT GTCTATGGCA TCTTGTGCTA GTGGAAGTAC ACGAGGCTAT 
GATGAATTAG TGCCTCATCA GATATCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGGAG CATTGCCATC AAATGTGGGT GATGTTAATT TCCAGAGTGG AATTATTGCA 
GCCAGATGTG CTATCAATAA ACTTCATCAA GAGCTCGGAG CCAAAGGTTT TATTCAGGTA 
TATGTAGATC AAGTTGATGA AGACATAGTG GCAGTGACCA GACATTCACC TAGTATCCAT 
CAGTCCGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAAACTTC GTTTTACAGC 
AAGGAAGTAC CTCAGATGTG CATCCCTGGC AAAATTGAAG AGGTTGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAACACACA ACCATATGTA AAGGATGAGC ATTCAATCAA CGGGCTGCCA 
AACATCACAG TAGAAATTAG GGAACATATT CAGCTCAAAG AAAGTAAAAT TGTAAAACAA 
GCTGGAATTG CCACAAAAGG TCCAAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATCGT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GGAACCATCT GACACAGTTC AGTCCGCACT TTAAATCTGG AAGCCTTGCT 
GTTGACAACT CGGATCCTAT TTTGAAAATC CCATTTGCTT CTATTGCCTC TAACTTGACT 
TTGGCTGAGC TAAATCAAAT ACTTTACCGA TGTGAATCAG AGGAACAAGA AGATGGTGGA 
GGTTGTTACA ACATACCAAA CTGGTCATCC CTTAAATACG CAGGTCTTCA AGGATTAATG 
TCTGTATTGG CAGAAATTAG ACCAAAGAAT GATTTGGGGC ATCCTTTTTG TGATAATTTG 
AGATCCGGAG ATTGGATGAT TGACTATGTC AGTGGCCGGC TTATTTCACG ATCAGGAAAT 
ATTGCTGAAG TCGGCAAATG GTTACAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGA 
TATCTTATCC CATGTTATTT TGATGCAATA TTAATTGGTG TATATACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACATTTGT AAAACACCTT 
TCATTGGGGT CAGTTCAGAT GTGTGGAGTA GGAAAATTCC CTTGTCTGCC CCTTCTGTCT 
CCTTCACTTT GGGATGTGCC ACACAGGGTA AATGAGATCA CAAAAGAAAA GGAGCAGTGT 
TGTGCTTCTC TGGCTGCAGG CTTACCTCAT TTTTCTGCTG GTCTTTTCCG CTGTTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTCTT TTGCTGGTTA CTGGACGCTA CTTAGAGGCC 
AGGAACATTA TTTTAGCATT CGCTGGTACT CTGAGGCATG GTCTTATCCC CAACCTCCTT 
GGTGAAGGAG TTTATGCCAG GTACAATTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGATT ACTGTAAGAT CGTCCCGAAC GGTCTAGACA TTCTCAAGTG TCCAGTGTCC 
AGGATGTATC CAACAGATGA TTCTGCTCCT TTGCCTGCTG GTACACTGGA TCAACCACTA 
TTTGAAGTGA TACAAGAAAC TATGCAAAGA CATATGCAGG GCATACAGTT CCGAGAGAGA 
AATGCTGGCT CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT AACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGT GGAAATCGCT TCAATTGTGG CACGTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TGGAAATTGT AGGCCTTGCC AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTTCC TTATCATGAA GTTAGAATAA AAAGACACGG GAAAGTTGTA 
GCCGTCTCAT ATGATGAGTG GAACAGGAAA ATACAAGACA ACTTTGAAAA GCTGTTTCAT 
GTTTCGGAAG ACCCTTCAGA CCCTAATGAG AAGCATCCAA ATCTAGTCCA CAAACGGGGC 
ATCTACAAAG ATAGTTACGG AGCTTCCAGT CCTTGGTGTG ACTACCAGCT CAGGCCGAAC 
TTTACCATCG CAATGGTCGT GGCCCCTGAG CTCTTTACTG CAGGAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTGGGC CCCCTAGGCA TGAAAACCTT AGATCCAGAT 
GATATGGTGT ACTGTGGAAT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AGAGGTTTCA ATTATCATCA AGGACCTGAG TGGCTATGGC CTGTTGGATA TTTCCTTCGT 
GCAAAGTTAT ATTTTGCCAA ATTGATGGGT CCAGAGACTA CTGCAAAAAC TGTGTTTTTG 
GTCAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCTATTG GAAAGGTCTT 
CCAGAACTGA CCAATGAAAA TGGCCAATAC TGTCCTTTCA GCTGTGAGAC ACAAGCTTGG 
TCAATTGCTA CCGTTCTTGA AACACTTTAT GATTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agl ( XM_013142991.1 ) cDNA ORF clone, Cavia porcellus(domestic guinea pig) -> XP_012998445.1 Cavia porcellus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X1, mRNA.

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CloneID	ODo24114
Clone ID Related Accession (Same CDS sequence)	XM_013142992.2 , XM_013142991.2 , XM_013142992.1 , XM_003479171.3 , XM_003479171.4 , XM_013142991.1
Accession Version	XM_013142991.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436716800000
Organism	Cavia porcellus(domestic guinea pig)
Product	glycogen debranching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176417.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cavia porcellus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACACA GTAAACAGAT TCGAATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTTTTCA GACTTGAGCA AGGGTTTGAG CTACAGTTCC GATTGGGCCC AACTTTGCAG 
GGAAAACCAG TTACTGTATA TACAAATTAT CCATTTCCTG GAGAAACATT TAATCGACAT 
AAATTCCGTT CCCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAGCA GTCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAGAGTGGTG GAGGTTACAT AGTCGTGGAT CCTATTTTAC GAGTTGGTGC TGATAGCCAT 
GTGTTACCCT TGGATTGTGT TACTCTGCAG ACATTTTTAG CTAAGTGTTT AGGACCTTTT 
GATGAATGGG AAAGCAGAAT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCACTTC 
ACCCCGCTGC AGACTCTTGG ACTGTCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGATAGA AAGTACACCT GGCATGATGT TGGGCAACTG 
GTAGAAAAAC TGAAGAAGGA GTGGAATGTT CTTTGTATTA CAGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GCTCCAGGAA CACCCAGAGT CTGCATATAA TCTTGTGAAT 
TCTCCACATT TAAAACCGGC CTGGATTTTA GACAGAGCAC TTTGGCATTT CTCCTGTGAG 
GTTGCAGAAG GGAAATATAG AGAAAAAGGA ATCCCTGCTT TGATTGAAAA TGATCACCAC 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATGTTTACC CAAAGATTAA ACTGTGGGAA 
TTTTTCCAAG TACATGAACA CAGAGCAGTT GAGGAATTTA GAAGGCTGCT CACACAAGAA 
AATAGAAGAA CAACCAAGTC TGATCCAAAA CAACATCTTA AGATTATTCA GGATCCTGAA 
TATAGACGCC TTGGCTGTAC TATAGATATG AACATTGCAT TGGCAACTTT CATACCACAT 
AACAATGGAC CAGCCGCAAT TGAAGAATGC TGTAATTGGT TTCGTAAGAA AATTGAGGAA 
TTAAATTCAG AGAAATATCA ACTCATACAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGAATGGCT GGCCATGGTC CTAAACTAGG ACCTGTCACT 
AGAAAACATC CCTTAGTTAC CAGGTATTTT ACTTTCCCAT TCCAAGAAAT GGACTTATCC 
ACAGAAGAGT CCATGATGCA CCTCCCTGAT AAAGCTTGTT TCTTCATGGC ACATAATGGA 
TGGGTGATGG GAGATGACCC TCTTCGAAAC TTTGCTGAGC CAGGTTCAGA TGTTTACTTA 
AGGCGAGAAC TTATATGCTG GGGCGACAGT GTTAAATTGC GCTACGGAAA TAAGCCAGAG 
GATTGTCCTT ACCTATGGGC ACACATGAAA AAATACACTG AAATCACTGC GACTTATTTC 
CAGGGTGTAC GTCTTGATAA CTGCCACTCA ACACCTATTC ATGTGGCTGA GTACATGTTG 
GATGCTGCTA GGAAATTACA ACCCAATTTA TATGTAGTAG CTGAACTATT CACAGGAAGT 
GAAGAGCTGG ACAATATCTT TGTCACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCG 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG TGGAGAACCG 
GTTGGATCCT TTGTTCAGCC CTGTCTGAGG CCTTTAATGC CAGCTATCGC ACATGCCCTG 
TTTATGGATA TTACCCATGA TAACGAGTGT CCTATTGTGC ATAGATCAGC CTACGATGCT 
CTTCCAAGTA CCACAATTGT GTCTATGGCA TCTTGTGCTA GTGGAAGTAC ACGAGGCTAT 
GATGAATTAG TGCCTCATCA GATATCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGGAG CATTGCCATC AAATGTGGGT GATGTTAATT TCCAGAGTGG AATTATTGCA 
GCCAGATGTG CTATCAATAA ACTTCATCAA GAGCTCGGAG CCAAAGGTTT TATTCAGGTA 
TATGTAGATC AAGTTGATGA AGACATAGTG GCAGTGACCA GACATTCACC TAGTATCCAT 
CAGTCCGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAAACTTC GTTTTACAGC 
AAGGAAGTAC CTCAGATGTG CATCCCTGGC AAAATTGAAG AGGTTGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAACACACA ACCATATGTA AAGGATGAGC ATTCAATCAA CGGGCTGCCA 
AACATCACAG TAGAAATTAG GGAACATATT CAGCTCAAAG AAAGTAAAAT TGTAAAACAA 
GCTGGAATTG CCACAAAAGG TCCAAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATCGT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GGAACCATCT GACACAGTTC AGTCCGCACT TTAAATCTGG AAGCCTTGCT 
GTTGACAACT CGGATCCTAT TTTGAAAATC CCATTTGCTT CTATTGCCTC TAACTTGACT 
TTGGCTGAGC TAAATCAAAT ACTTTACCGA TGTGAATCAG AGGAACAAGA AGATGGTGGA 
GGTTGTTACA ACATACCAAA CTGGTCATCC CTTAAATACG CAGGTCTTCA AGGATTAATG 
TCTGTATTGG CAGAAATTAG ACCAAAGAAT GATTTGGGGC ATCCTTTTTG TGATAATTTG 
AGATCCGGAG ATTGGATGAT TGACTATGTC AGTGGCCGGC TTATTTCACG ATCAGGAAAT 
ATTGCTGAAG TCGGCAAATG GTTACAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGA 
TATCTTATCC CATGTTATTT TGATGCAATA TTAATTGGTG TATATACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACATTTGT AAAACACCTT 
TCATTGGGGT CAGTTCAGAT GTGTGGAGTA GGAAAATTCC CTTGTCTGCC CCTTCTGTCT 
CCTTCACTTT GGGATGTGCC ACACAGGGTA AATGAGATCA CAAAAGAAAA GGAGCAGTGT 
TGTGCTTCTC TGGCTGCAGG CTTACCTCAT TTTTCTGCTG GTCTTTTCCG CTGTTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTCTT TTGCTGGTTA CTGGACGCTA CTTAGAGGCC 
AGGAACATTA TTTTAGCATT CGCTGGTACT CTGAGGCATG GTCTTATCCC CAACCTCCTT 
GGTGAAGGAG TTTATGCCAG GTACAATTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGATT ACTGTAAGAT CGTCCCGAAC GGTCTAGACA TTCTCAAGTG TCCAGTGTCC 
AGGATGTATC CAACAGATGA TTCTGCTCCT TTGCCTGCTG GTACACTGGA TCAACCACTA 
TTTGAAGTGA TACAAGAAAC TATGCAAAGA CATATGCAGG GCATACAGTT CCGAGAGAGA 
AATGCTGGCT CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT AACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGT GGAAATCGCT TCAATTGTGG CACGTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TGGAAATTGT AGGCCTTGCC AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTTCC TTATCATGAA GTTAGAATAA AAAGACACGG GAAAGTTGTA 
GCCGTCTCAT ATGATGAGTG GAACAGGAAA ATACAAGACA ACTTTGAAAA GCTGTTTCAT 
GTTTCGGAAG ACCCTTCAGA CCCTAATGAG AAGCATCCAA ATCTAGTCCA CAAACGGGGC 
ATCTACAAAG ATAGTTACGG AGCTTCCAGT CCTTGGTGTG ACTACCAGCT CAGGCCGAAC 
TTTACCATCG CAATGGTCGT GGCCCCTGAG CTCTTTACTG CAGGAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTGGGC CCCCTAGGCA TGAAAACCTT AGATCCAGAT 
GATATGGTGT ACTGTGGAAT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AGAGGTTTCA ATTATCATCA AGGACCTGAG TGGCTATGGC CTGTTGGATA TTTCCTTCGT 
GCAAAGTTAT ATTTTGCCAA ATTGATGGGT CCAGAGACTA CTGCAAAAAC TGTGTTTTTG 
GTCAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCTATTG GAAAGGTCTT 
CCAGAACTGA CCAATGAAAA TGGCCAATAC TGTCCTTTCA GCTGTGAGAC ACAAGCTTGG 
TCAATTGCTA CCGTTCTTGA AACACTTTAT GATTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012998445.1
CDS	309..4907
Translation	MEHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKPVTVYTNYPFPGETFNRHKFRSLDWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADSHVLPLDCVTLQTFLAKCLGPFDEWESRIRVAKESGYNMIHFTPLQTLGLSRSCYSLANQLELNPDFSRPDRKYTWHDVGQLVEKLKKEWNVLCITDVVYNHTAANSKWLQEHPESAYNLVNSPHLKPAWILDRALWHFSCEVAEGKYREKGIPALIENDHHMNCIRKVIWEDVYPKIKLWEFFQVHEHRAVEEFRRLLTQENRRTTKSDPKQHLKIIQDPEYRRLGCTIDMNIALATFIPHNNGPAAIEECCNWFRKKIEELNSEKYQLIHYHQEQAVNCLLGNVFYERMAGHGPKLGPVTRKHPLVTRYFTFPFQEMDLSTEESMMHLPDKACFFMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITATYFQGVRLDNCHSTPIHVAEYMLDAARKLQPNLYVVAELFTGSEELDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSAYDALPSTTIVSMASCASGSTRGYDELVPHQISVVSEERFYTKWNPGALPSNVGDVNFQSGIIAARCAINKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPGKIEEVVLEARTVERNTQPYVKDEHSINGLPNITVEIREHIQLKESKIVKQAGIATKGPNEYIQEIEFENLSPGSVIVFRVSLDPHAQVAVGILRNHLTQFSPHFKSGSLAVDNSDPILKIPFASIASNLTLAELNQILYRCESEEQEDGGGCYNIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSGRLISRSGNIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGVYTTLLDIAWKQMSSFVQNGSTFVKHLSLGSVQMCGVGKFPCLPLLSPSLWDVPHRVNEITKEKEQCCASLAAGLPHFSAGLFRCWGRDTFIALRGLLLVTGRYLEARNIILAFAGTLRHGLIPNLLGEGVYARYNCRDAVWWWLQCIQDYCKIVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFEVIQETMQRHMQGIQFRERNAGSQIDRNMKDEGFNITAGVDEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLAKSAVRWLLELSKKNIFPYHEVRIKRHGKVVAVSYDEWNRKIQDNFEKLFHVSEDPSDPNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAGKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLARGFNYHQGPEWLWPVGYFLRAKLYFAKLMGPETTAKTVFLVKNVLSRHYVHLERSYWKGLPELTNENGQYCPFSCETQAWSIATVLETLYDL

Target ORF information:

RefSeq Version	XM_013142991.1
Organism	Cavia porcellus(domestic guinea pig)
Definition	Cavia porcellus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (Agl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013142991.1

ORF Insert Sequence:

ATGGAACACA GTAAACAGAT TCGAATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTTTTCA GACTTGAGCA AGGGTTTGAG CTACAGTTCC GATTGGGCCC AACTTTGCAG 
GGAAAACCAG TTACTGTATA TACAAATTAT CCATTTCCTG GAGAAACATT TAATCGACAT 
AAATTCCGTT CCCTGGATTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGTAAACTTA ATCTGCAGCA GTCTGGATCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAGAGTGGTG GAGGTTACAT AGTCGTGGAT CCTATTTTAC GAGTTGGTGC TGATAGCCAT 
GTGTTACCCT TGGATTGTGT TACTCTGCAG ACATTTTTAG CTAAGTGTTT AGGACCTTTT 
GATGAATGGG AAAGCAGAAT TAGGGTTGCA AAAGAATCAG GCTACAACAT GATTCACTTC 
ACCCCGCTGC AGACTCTTGG ACTGTCTAGG TCATGCTACT CCCTTGCCAA TCAGTTAGAA 
TTAAATCCTG ACTTTTCAAG ACCTGATAGA AAGTACACCT GGCATGATGT TGGGCAACTG 
GTAGAAAAAC TGAAGAAGGA GTGGAATGTT CTTTGTATTA CAGATGTTGT CTACAATCAT 
ACTGCTGCTA ACAGTAAATG GCTCCAGGAA CACCCAGAGT CTGCATATAA TCTTGTGAAT 
TCTCCACATT TAAAACCGGC CTGGATTTTA GACAGAGCAC TTTGGCATTT CTCCTGTGAG 
GTTGCAGAAG GGAAATATAG AGAAAAAGGA ATCCCTGCTT TGATTGAAAA TGATCACCAC 
ATGAATTGCA TTCGAAAAGT AATTTGGGAG GATGTTTACC CAAAGATTAA ACTGTGGGAA 
TTTTTCCAAG TACATGAACA CAGAGCAGTT GAGGAATTTA GAAGGCTGCT CACACAAGAA 
AATAGAAGAA CAACCAAGTC TGATCCAAAA CAACATCTTA AGATTATTCA GGATCCTGAA 
TATAGACGCC TTGGCTGTAC TATAGATATG AACATTGCAT TGGCAACTTT CATACCACAT 
AACAATGGAC CAGCCGCAAT TGAAGAATGC TGTAATTGGT TTCGTAAGAA AATTGAGGAA 
TTAAATTCAG AGAAATATCA ACTCATACAC TATCATCAGG AACAGGCAGT TAATTGCCTT 
TTGGGAAATG TGTTTTATGA ACGAATGGCT GGCCATGGTC CTAAACTAGG ACCTGTCACT 
AGAAAACATC CCTTAGTTAC CAGGTATTTT ACTTTCCCAT TCCAAGAAAT GGACTTATCC 
ACAGAAGAGT CCATGATGCA CCTCCCTGAT AAAGCTTGTT TCTTCATGGC ACATAATGGA 
TGGGTGATGG GAGATGACCC TCTTCGAAAC TTTGCTGAGC CAGGTTCAGA TGTTTACTTA 
AGGCGAGAAC TTATATGCTG GGGCGACAGT GTTAAATTGC GCTACGGAAA TAAGCCAGAG 
GATTGTCCTT ACCTATGGGC ACACATGAAA AAATACACTG AAATCACTGC GACTTATTTC 
CAGGGTGTAC GTCTTGATAA CTGCCACTCA ACACCTATTC ATGTGGCTGA GTACATGTTG 
GATGCTGCTA GGAAATTACA ACCCAATTTA TATGTAGTAG CTGAACTATT CACAGGAAGT 
GAAGAGCTGG ACAATATCTT TGTCACTAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCG 
ATGAGTGCAT ATAATAGTCA TGAAGAGGGC AGATTAGTTT ACCGATATGG TGGAGAACCG 
GTTGGATCCT TTGTTCAGCC CTGTCTGAGG CCTTTAATGC CAGCTATCGC ACATGCCCTG 
TTTATGGATA TTACCCATGA TAACGAGTGT CCTATTGTGC ATAGATCAGC CTACGATGCT 
CTTCCAAGTA CCACAATTGT GTCTATGGCA TCTTGTGCTA GTGGAAGTAC ACGAGGCTAT 
GATGAATTAG TGCCTCATCA GATATCAGTG GTTTCTGAAG AACGGTTTTA CACTAAGTGG 
AATCCTGGAG CATTGCCATC AAATGTGGGT GATGTTAATT TCCAGAGTGG AATTATTGCA 
GCCAGATGTG CTATCAATAA ACTTCATCAA GAGCTCGGAG CCAAAGGTTT TATTCAGGTA 
TATGTAGATC AAGTTGATGA AGACATAGTG GCAGTGACCA GACATTCACC TAGTATCCAT 
CAGTCCGTTG TGGCTGTGTC TAGAACTGCT TTCAGGAATC CCAAAACTTC GTTTTACAGC 
AAGGAAGTAC CTCAGATGTG CATCCCTGGC AAAATTGAAG AGGTTGTTCT TGAAGCTAGA 
ACTGTTGAGA GAAACACACA ACCATATGTA AAGGATGAGC ATTCAATCAA CGGGCTGCCA 
AACATCACAG TAGAAATTAG GGAACATATT CAGCTCAAAG AAAGTAAAAT TGTAAAACAA 
GCTGGAATTG CCACAAAAGG TCCAAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATCGT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GGAACCATCT GACACAGTTC AGTCCGCACT TTAAATCTGG AAGCCTTGCT 
GTTGACAACT CGGATCCTAT TTTGAAAATC CCATTTGCTT CTATTGCCTC TAACTTGACT 
TTGGCTGAGC TAAATCAAAT ACTTTACCGA TGTGAATCAG AGGAACAAGA AGATGGTGGA 
GGTTGTTACA ACATACCAAA CTGGTCATCC CTTAAATACG CAGGTCTTCA AGGATTAATG 
TCTGTATTGG CAGAAATTAG ACCAAAGAAT GATTTGGGGC ATCCTTTTTG TGATAATTTG 
AGATCCGGAG ATTGGATGAT TGACTATGTC AGTGGCCGGC TTATTTCACG ATCAGGAAAT 
ATTGCTGAAG TCGGCAAATG GTTACAGGCT ATGTTCTTCT ACCTGAAGCA GATTCCACGA 
TATCTTATCC CATGTTATTT TGATGCAATA TTAATTGGTG TATATACCAC TCTTCTGGAT 
ATAGCATGGA AGCAGATGTC AAGCTTTGTT CAGAATGGTT CAACATTTGT AAAACACCTT 
TCATTGGGGT CAGTTCAGAT GTGTGGAGTA GGAAAATTCC CTTGTCTGCC CCTTCTGTCT 
CCTTCACTTT GGGATGTGCC ACACAGGGTA AATGAGATCA CAAAAGAAAA GGAGCAGTGT 
TGTGCTTCTC TGGCTGCAGG CTTACCTCAT TTTTCTGCTG GTCTTTTCCG CTGTTGGGGA 
AGGGATACTT TTATTGCACT TAGAGGTCTT TTGCTGGTTA CTGGACGCTA CTTAGAGGCC 
AGGAACATTA TTTTAGCATT CGCTGGTACT CTGAGGCATG GTCTTATCCC CAACCTCCTT 
GGTGAAGGAG TTTATGCCAG GTACAATTGC CGGGATGCTG TGTGGTGGTG GCTGCAGTGT 
ATTCAGGATT ACTGTAAGAT CGTCCCGAAC GGTCTAGACA TTCTCAAGTG TCCAGTGTCC 
AGGATGTATC CAACAGATGA TTCTGCTCCT TTGCCTGCTG GTACACTGGA TCAACCACTA 
TTTGAAGTGA TACAAGAAAC TATGCAAAGA CATATGCAGG GCATACAGTT CCGAGAGAGA 
AATGCTGGCT CACAGATAGA TCGGAACATG AAGGATGAAG GTTTTAATAT AACTGCAGGG 
GTTGATGAAG AAACAGGATT TGTTTATGGT GGAAATCGCT TCAATTGTGG CACGTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCTAGA AACAGAGGAA TCCCAGCCAC TCCAAGAGAT 
GGGTCTGCTG TGGAAATTGT AGGCCTTGCC AAATCTGCTG TTCGCTGGTT GCTGGAATTA 
TCCAAAAAAA ATATTTTTCC TTATCATGAA GTTAGAATAA AAAGACACGG GAAAGTTGTA 
GCCGTCTCAT ATGATGAGTG GAACAGGAAA ATACAAGACA ACTTTGAAAA GCTGTTTCAT 
GTTTCGGAAG ACCCTTCAGA CCCTAATGAG AAGCATCCAA ATCTAGTCCA CAAACGGGGC 
ATCTACAAAG ATAGTTACGG AGCTTCCAGT CCTTGGTGTG ACTACCAGCT CAGGCCGAAC 
TTTACCATCG CAATGGTCGT GGCCCCTGAG CTCTTTACTG CAGGAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTGGGC CCCCTAGGCA TGAAAACCTT AGATCCAGAT 
GATATGGTGT ACTGTGGAAT TTATGACAAT GCCTTAGACA ATGACAACTA CAATCTTGCT 
AGAGGTTTCA ATTATCATCA AGGACCTGAG TGGCTATGGC CTGTTGGATA TTTCCTTCGT 
GCAAAGTTAT ATTTTGCCAA ATTGATGGGT CCAGAGACTA CTGCAAAAAC TGTGTTTTTG 
GTCAAAAATG TTCTTTCCCG ACATTATGTT CATCTTGAGA GATCCTATTG GAAAGGTCTT 
CCAGAACTGA CCAATGAAAA TGGCCAATAC TGTCCTTTCA GCTGTGAGAC ACAAGCTTGG 
TCAATTGCTA CCGTTCTTGA AACACTTTAT GATTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Agl cDNA ORF clone, Cavia porcellus(domestic guinea pig)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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