EGF cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	EGF
Entrez Gene ID	403657
Full Name	epidermal growth factor
Synonyms	CEGF
General protein information	Preferred Names epidermal growth factor Names pro-epidermal growth factor epidermal growth factor (beta-urogastrone)
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014109884.2	XP_013965359.1	pro-epidermal growth factor isoform X1
XM_022413384.1	XP_022269092.1	pro-epidermal growth factor isoform X2
NM_001003094.1	NP_001003094.1	pro-epidermal growth factor precursor
XM_014109884.1	XP_013965359.1	pro-epidermal growth factor isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	EGF	NP_001954.2
Bos taurus (cattle)	EGF	XP_002688149.2
Mus musculus (house mouse)	Egf	NP_034243.2
Macaca mulatta (Rhesus monkey)	EGF	XP_001088957.1
Rattus norvegicus (Norway rat)	Egf	NP_036974.1
Canis lupus familiaris (dog)	EGF	NP_001003094.1
Danio rerio (zebrafish)	egf	NP_991294.1
Pan troglodytes (chimpanzee)	EGF	XP_517395.2
Gallus gallus (chicken)	EGF	NP_001001292.1
Xenopus tropicalis (tropical clawed frog)	egf	NP_001106478.1

Gene Ontology
Bibliography

Related articles in PubMed

FGF2 and EGF Are Required for Self-Renewal and Organoid Formation of Canine Normal and Tumor Breast Stem Cells.
Cocola C, Molgora S, Piscitelli E, Veronesi MC, Greco M, Bragato C, Moro M, Crosti M, Gray B, Milanesi L, Grieco V, Luvoni GC, Kehler J, Bellipanni G, Reinbold R, Zucchi I, Giordano A
Journal of cellular biochemistry118(3)570-584(2017 03)

Expression and localization of epidermal growth factor, transforming growth factor-α and epidermal growth factor receptor in the canine testis.
Tamada H, Takemoto K, Tominaga M, Kawate N, Takahashi M, Hatoya S, Matsuyama S, Inaba T, Sawada T
The Journal of reproduction and development62(1)59-64(2016)

Matrix stiffening sensitizes epithelial cells to EGF and enables the loss of contact inhibition of proliferation.
Kim JH, Asthagiri AR
Journal of cell science124(Pt 8)1280-7(2011 Apr)

Positive correlation of steroid hormones and EGF in canine mammary cancer.
Queiroga FL, Pérez-Alenza D, Silvan G, Peña L, Illera JC
The Journal of steroid biochemistry and molecular biology115(1-2)9-13(2009 May)

Epithelial growth factor-induced phosphorylation of caveolin 1 at tyrosine 14 stimulates caveolae formation in epithelial cells.
Orlichenko L, Huang B, Krueger E, McNiven MA
The Journal of biological chemistry281(8)4570-9(2006 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EGF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EGF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe00135	NM_001003094.1 Latest version!	Canis lupus familiaris epidermal growth factor (EGF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCe284002	XM_022413384.1 Latest version!	Canis lupus familiaris epidermal growth factor (EGF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OCe44531	XM_014109884.2 Latest version!	Canis lupus familiaris epidermal growth factor (EGF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OCe44531	XM_014109884.1	Canis lupus familiaris epidermal growth factor (EGF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EGF ( NM_001003094.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> NP_001003094.1 Canis lupus familiaris epidermal growth factor (EGF), mRNA.

Price & Availability↑

CloneID	OCe00135
Clone ID Related Accession (Same CDS sequence)	NM_001003094.1
Accession Version	NM_001003094.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550160000000
Organism	Canis lupus familiaris(dog)
Product	pro-epidermal growth factor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB049597.1. ##Evidence-Data-START## Transcript exon combination :: AB049597.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4547742, SAMN00013596 [ECO:0000348] ##Evidence-Data-END##

ATGCTGCTCC CCCTTATCAT TCTGTGGCCG GTAGTTTTTA AATGTAGTTT TGCTAGTCTC 
TCAGACCCGG AGAACTGGAA CTGTCCTGAA GTCTCTCCCT CAGGAAAGGG GAGCCCTGCT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA TCAGTATCTT TAGGATTGAC 
CTGGAAGGCA CTAATCATGA GCAATTGGTG GCAGATGCTG GTGTATCAGT GATCATGGAT 
TTTCATTATA ATAAAGAAAG AATCTATTGG GTAGATCCAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGACAAG GCAAGAGAGA GTATGCAATA TAGAGAAAAA TGTTTCAGGA 
ATGGCAATAA ATTGGATAAA TGAAGAACTT ATTTGGTCAA ATCAACAGGA AGGAATCATC 
ACAGTAACAG ATATGAAAGG AAACAATTCG CGAGTTCTCT TAAGAGCCTT AAACTATCCT 
GCAAATGTAG CAATTGATCC AATAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCAGTG 
GCTGGCAGCC TTCACAGAGC AGATCTCAAT GGTGTGGAAG AGAAGATTCT TTTACAGACA 
TCAGAAAGAA TAACAGCTGT GTCATTGGAT GTGCTTGATA AACAGCTTTT TTGGATTCAG 
TACAGCAGAG ATGGAAGCAA TTCTCATATT TATTCCTGTA ATTATGATGG AGGTTCTGTC 
CATCTTAGCA AACATCTTAC ACAGCATAAT TTTTTTGCAA TGTCCCTTTT TGGGAATCAG 
ATCTTCTATT CAACATGGAA AAAGAAGACA ATTTGGATAG CTAACAAACA CAGTGGGAAG 
GATATGGTTA GAATTAACCT AGATTCATCA TTTGTACCAC CTGGTGGAAT CAAAGTAGTG 
CATCCACTCT TACAGCCCAA GGCAGAGAGT GGCACTTGGG CACCTGATCA GAAACTCTGC 
AAATGGAAGC AAGGTAACTG CAGAGGCAGC ACGTGTGGTC AAGATTCAAA GTCCTATTCA 
TGCACGTGTG CAGAGGGATA TACTTTAAGC CAAGATGGAA AATACTGTGA AGATGTCAAT 
GAATGTGCCT TTTGGAATCA TGGCTGTACT CTTGGGTGTG AAAACATCCC TGGATCTTAT 
TATTGCACAT GCCCTGTAGG ATTTATTCTG CTTCCTGATG GGAAACGGTG TCATCAATTA 
ATTGCCTGTC CGAGCAATAC ATCTAAATGT AGCCATGACT GTGTTCTGAC ATCAGATGGT 
CCCATATGTT TCTGTCCTGA AGGCTCAGTG CTTGAGGCAG ATGGAAAAAC ATGTAGTGGC 
TGTTCATCAC CTGATAATGG TGGATGTAGC CAGCTCTGCC TCCCTCTCAG CCCAGTATCC 
TGGGAATGTG GCTGCTTTCC TGGGTATGAC CTACAACTGG ACAAACAAAG CTGTGCAGCA 
TCAGGACCAC AACCATTTTT GCTGTTTGCC AATTCTCAAG ATATTCGACA CATGCATTTT 
GATGGAACAG ATTATGGAAC CCTGCTCAGC CAGCAAATGG GAATGGTTTT TGCCCTTGAT 
CACGACCCTG TGGAAAATAA GATATACTTT GCCCATACAG CCCTGAAGTG GATAGAGAGA 
GCTAACATGG ATGGTTCCCA GCGAGAAAGG CTTATTGAGG AAGGAGTGGA TGTGCCAGAA 
GGTCTTGCCA TAGATTGGAT TGACCGTAAA TTCTACTGGA CAGACAGCGG AAAATCTCTT 
ATTGAAGGGA GTGATTTAAA TGGAAAACAT CGTGAGATAA TCATCAAGGA GGACATCTCT 
CAGCCACGAG GAATTGCTGT TCATCCAATG GCCAAGAGAT TATTCTGGAC TGATATGGGG 
ATTAATCCAC GAATTGAAAG TTCTTCCCTT CAAGGCATTG GCCGACTGGT TATAGCTAGC 
TCTGATCTGG TCTGGCCCAG TGGAATAACG ATTGATTATG TAACTGACAA ATTGTACTGG 
TGTGATACCA AGCTGTCTGT GATTGAGATG GCCAATCTGG ATGGTTCAAA ACGCCAAAGA 
CTTGCCCAGA ACGATGTAGG TCACCCATTT GCTATGGCCG TGTTTGAGGA TCACGTGTGG 
TTCTCTGATT GGACTATGCC ATCAATAATA AGAGTGGACA AGAGGACTGG CAAAAACAGG 
GTACGTCTCC GAGGCAGCAT GCTGAAGCCT TCATCACTGG TTGTAGTTCA TCCATTGGCA 
AAACCAGGAG CACAGCCCTG CTTATATCAA AATGGAGGCT GTGAACATAT TTGCAAAGAG 
AGGTTTGGAA CTGCTCAATG TTTGTGTCGT GAAGGTTTTG TGAAAGCCCC AGATGGGAAA 
ATGTGTCTGG CTCTGAATGG CCATCAGATA CCGGCAGTAG GTAGTGAAGC AGATCTAAGT 
AATCACGTAA CGCCAGGGGA TGTCTTACCC AGAAGTGAAG GATTTGAAGA TAACATTACA 
GAATCTCAGC ATATGCTAGT GGCCGAAATC ATGGTGTCAG ATGCTGACGA CTGTGCTCCT 
GTGGGATGCA GTACATGGGC TGAGTGTGTT TCAGAGGGAG AAAATGCCAC ATGTCAGTGT 
TTGAAAGGAT TTACTGGGGA TGGAAAGCTA TGTTTTGACA TAGATGAATG TGAGATGGGC 
ATCACGATTT GCCCTCCTAC CTCCTCAAAG TGCGTCAATA CTGAAGGTGG TTATGTTTGT 
CAGTGCTCAG AAGGCTACCG AGGCGATGGG ATCCACTGTC TGGATATTAA TGAGTGCCAA 
CTGGGCATGC ACACCTGTGG GGAAAATGCC ACCTGTACAA ATATGGAGGG AAACTATACC 
TGCATGTGCG CTGGCAGCCT GTCTGAACCT GGACAGATAT GTGCTGACTC TACTCCGCCT 
TCTCATCCCA TGGAGGACAG TCACTATTCT GTGAGAAATG GTTATCGGGA ATGCCCCTCA 
TCCTATGACG GGTACTGCCT CTATAATGGT GTGTGTATGT ACATTGAAGC AGTCGACAGA 
TACGCATGCA ACTGTGTTTT TGGCTACGTC GGGGAGCGAT GTCAGCACCG AGACCTGAAA 
TGGGAACTGC GCCACGCGGG CCAGGGCCGG CAGCGGCAGG TCGCCGCGGT GGCCGTGGGC 
GTGGTCGTGC TCGTCCTGCT GCTGCTGCTC GGGCTGGGGG GCGCGCACTG CTACAGGACT 
AAGAAGTTGT CATCAAAAAA TTTAAAGAAT CCTTATGAAG AGCCAAGCAG AGAGGGTAGC 
AGTAGCAGGC CTTCAGACAG CGAGGCTAGG ATGGCCTCTT TTCCCCAACC TTGGTTTGTG 
GTTATAAAGG AACATCAAAA TCTCAGGAAT GGAAGTCAAC CTATGGCCCT CAAGGATGGT 
GAGTCAGCAG ATGTTAGCCA ATTTTCCTCT CCAGAGCCAG GGTCAGTAAA ACGGACCTCA 
TGGAGAAATG AACACCAGTT ATATAAGGAC ACAGAGCAAG GCTGCTGCAC TCCACCATCC 
AGTAATAGAG GCACCGGCTC TCAGTCAATG GAGCAGAGCT TTTCTGTCCC CTCCTATGAG 
GCACAGCCCA TTGCTTTGGG GGTTGAGAAG CCACAGTCTC TCCTATCAGC TAAACCCTTA 
TTGCAACAAA GGGCCCCAGA TCCACCACAC CAAATGAAGC TGATTCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003094.1
CDS	1..3651
Translation	MLLPLIILWPVVFKCSFASLSDPENWNCPEVSPSGKGSPACVGPAPFLIFSHGISIFRIDLEGTNHEQLVADAGVSVIMDFHYNKERIYWVDPERQLLQRVFLNGTRQERVCNIEKNVSGMAINWINEELIWSNQQEGIITVTDMKGNNSRVLLRALNYPANVAIDPIERFIFWSSEVAVAGSLHRADLNGVEEKILLQTSERITAVSLDVLDKQLFWIQYSRDGSNSHIYSCNYDGGSVHLSKHLTQHNFFAMSLFGNQIFYSTWKKKTIWIANKHSGKDMVRINLDSSFVPPGGIKVVHPLLQPKAESGTWAPDQKLCKWKQGNCRGSTCGQDSKSYSCTCAEGYTLSQDGKYCEDVNECAFWNHGCTLGCENIPGSYYCTCPVGFILLPDGKRCHQLIACPSNTSKCSHDCVLTSDGPICFCPEGSVLEADGKTCSGCSSPDNGGCSQLCLPLSPVSWECGCFPGYDLQLDKQSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVFALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAIDWIDRKFYWTDSGKSLIEGSDLNGKHREIIIKEDISQPRGIAVHPMAKRLFWTDMGINPRIESSSLQGIGRLVIASSDLVWPSGITIDYVTDKLYWCDTKLSVIEMANLDGSKRQRLAQNDVGHPFAMAVFEDHVWFSDWTMPSIIRVDKRTGKNRVRLRGSMLKPSSLVVVHPLAKPGAQPCLYQNGGCEHICKERFGTAQCLCREGFVKAPDGKMCLALNGHQIPAVGSEADLSNHVTPGDVLPRSEGFEDNITESQHMLVAEIMVSDADDCAPVGCSTWAECVSEGENATCQCLKGFTGDGKLCFDIDECEMGITICPPTSSKCVNTEGGYVCQCSEGYRGDGIHCLDINECQLGMHTCGENATCTNMEGNYTCMCAGSLSEPGQICADSTPPSHPMEDSHYSVRNGYRECPSSYDGYCLYNGVCMYIEAVDRYACNCVFGYVGERCQHRDLKWELRHAGQGRQRQVAAVAVGVVVLVLLLLLGLGGAHCYRTKKLSSKNLKNPYEEPSREGSSSRPSDSEARMASFPQPWFVVIKEHQNLRNGSQPMALKDGESADVSQFSSPEPGSVKRTSWRNEHQLYKDTEQGCCTPPSSNRGTGSQSMEQSFSVPSYEAQPIALGVEKPQSLLSAKPLLQQRAPDPPHQMKLIQ

Target ORF information:

RefSeq Version	NM_001003094.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris epidermal growth factor (EGF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003094.1

ORF Insert Sequence:

ATGCTGCTCC CCCTTATCAT TCTGTGGCCG GTAGTTTTTA AATGTAGTTT TGCTAGTCTC 
TCAGACCCGG AGAACTGGAA CTGTCCTGAA GTCTCTCCCT CAGGAAAGGG GAGCCCTGCT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA TCAGTATCTT TAGGATTGAC 
CTGGAAGGCA CTAATCATGA GCAATTGGTG GCAGATGCTG GTGTATCAGT GATCATGGAT 
TTTCATTATA ATAAAGAAAG AATCTATTGG GTAGATCCAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGACAAG GCAAGAGAGA GTATGCAATA TAGAGAAAAA TGTTTCAGGA 
ATGGCAATAA ATTGGATAAA TGAAGAACTT ATTTGGTCAA ATCAACAGGA AGGAATCATC 
ACAGTAACAG ATATGAAAGG AAACAATTCG CGAGTTCTCT TAAGAGCCTT AAACTATCCT 
GCAAATGTAG CAATTGATCC AATAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCAGTG 
GCTGGCAGCC TTCACAGAGC AGATCTCAAT GGTGTGGAAG AGAAGATTCT TTTACAGACA 
TCAGAAAGAA TAACAGCTGT GTCATTGGAT GTGCTTGATA AACAGCTTTT TTGGATTCAG 
TACAGCAGAG ATGGAAGCAA TTCTCATATT TATTCCTGTA ATTATGATGG AGGTTCTGTC 
CATCTTAGCA AACATCTTAC ACAGCATAAT TTTTTTGCAA TGTCCCTTTT TGGGAATCAG 
ATCTTCTATT CAACATGGAA AAAGAAGACA ATTTGGATAG CTAACAAACA CAGTGGGAAG 
GATATGGTTA GAATTAACCT AGATTCATCA TTTGTACCAC CTGGTGGAAT CAAAGTAGTG 
CATCCACTCT TACAGCCCAA GGCAGAGAGT GGCACTTGGG CACCTGATCA GAAACTCTGC 
AAATGGAAGC AAGGTAACTG CAGAGGCAGC ACGTGTGGTC AAGATTCAAA GTCCTATTCA 
TGCACGTGTG CAGAGGGATA TACTTTAAGC CAAGATGGAA AATACTGTGA AGATGTCAAT 
GAATGTGCCT TTTGGAATCA TGGCTGTACT CTTGGGTGTG AAAACATCCC TGGATCTTAT 
TATTGCACAT GCCCTGTAGG ATTTATTCTG CTTCCTGATG GGAAACGGTG TCATCAATTA 
ATTGCCTGTC CGAGCAATAC ATCTAAATGT AGCCATGACT GTGTTCTGAC ATCAGATGGT 
CCCATATGTT TCTGTCCTGA AGGCTCAGTG CTTGAGGCAG ATGGAAAAAC ATGTAGTGGC 
TGTTCATCAC CTGATAATGG TGGATGTAGC CAGCTCTGCC TCCCTCTCAG CCCAGTATCC 
TGGGAATGTG GCTGCTTTCC TGGGTATGAC CTACAACTGG ACAAACAAAG CTGTGCAGCA 
TCAGGACCAC AACCATTTTT GCTGTTTGCC AATTCTCAAG ATATTCGACA CATGCATTTT 
GATGGAACAG ATTATGGAAC CCTGCTCAGC CAGCAAATGG GAATGGTTTT TGCCCTTGAT 
CACGACCCTG TGGAAAATAA GATATACTTT GCCCATACAG CCCTGAAGTG GATAGAGAGA 
GCTAACATGG ATGGTTCCCA GCGAGAAAGG CTTATTGAGG AAGGAGTGGA TGTGCCAGAA 
GGTCTTGCCA TAGATTGGAT TGACCGTAAA TTCTACTGGA CAGACAGCGG AAAATCTCTT 
ATTGAAGGGA GTGATTTAAA TGGAAAACAT CGTGAGATAA TCATCAAGGA GGACATCTCT 
CAGCCACGAG GAATTGCTGT TCATCCAATG GCCAAGAGAT TATTCTGGAC TGATATGGGG 
ATTAATCCAC GAATTGAAAG TTCTTCCCTT CAAGGCATTG GCCGACTGGT TATAGCTAGC 
TCTGATCTGG TCTGGCCCAG TGGAATAACG ATTGATTATG TAACTGACAA ATTGTACTGG 
TGTGATACCA AGCTGTCTGT GATTGAGATG GCCAATCTGG ATGGTTCAAA ACGCCAAAGA 
CTTGCCCAGA ACGATGTAGG TCACCCATTT GCTATGGCCG TGTTTGAGGA TCACGTGTGG 
TTCTCTGATT GGACTATGCC ATCAATAATA AGAGTGGACA AGAGGACTGG CAAAAACAGG 
GTACGTCTCC GAGGCAGCAT GCTGAAGCCT TCATCACTGG TTGTAGTTCA TCCATTGGCA 
AAACCAGGAG CACAGCCCTG CTTATATCAA AATGGAGGCT GTGAACATAT TTGCAAAGAG 
AGGTTTGGAA CTGCTCAATG TTTGTGTCGT GAAGGTTTTG TGAAAGCCCC AGATGGGAAA 
ATGTGTCTGG CTCTGAATGG CCATCAGATA CCGGCAGTAG GTAGTGAAGC AGATCTAAGT 
AATCACGTAA CGCCAGGGGA TGTCTTACCC AGAAGTGAAG GATTTGAAGA TAACATTACA 
GAATCTCAGC ATATGCTAGT GGCCGAAATC ATGGTGTCAG ATGCTGACGA CTGTGCTCCT 
GTGGGATGCA GTACATGGGC TGAGTGTGTT TCAGAGGGAG AAAATGCCAC ATGTCAGTGT 
TTGAAAGGAT TTACTGGGGA TGGAAAGCTA TGTTTTGACA TAGATGAATG TGAGATGGGC 
ATCACGATTT GCCCTCCTAC CTCCTCAAAG TGCGTCAATA CTGAAGGTGG TTATGTTTGT 
CAGTGCTCAG AAGGCTACCG AGGCGATGGG ATCCACTGTC TGGATATTAA TGAGTGCCAA 
CTGGGCATGC ACACCTGTGG GGAAAATGCC ACCTGTACAA ATATGGAGGG AAACTATACC 
TGCATGTGCG CTGGCAGCCT GTCTGAACCT GGACAGATAT GTGCTGACTC TACTCCGCCT 
TCTCATCCCA TGGAGGACAG TCACTATTCT GTGAGAAATG GTTATCGGGA ATGCCCCTCA 
TCCTATGACG GGTACTGCCT CTATAATGGT GTGTGTATGT ACATTGAAGC AGTCGACAGA 
TACGCATGCA ACTGTGTTTT TGGCTACGTC GGGGAGCGAT GTCAGCACCG AGACCTGAAA 
TGGGAACTGC GCCACGCGGG CCAGGGCCGG CAGCGGCAGG TCGCCGCGGT GGCCGTGGGC 
GTGGTCGTGC TCGTCCTGCT GCTGCTGCTC GGGCTGGGGG GCGCGCACTG CTACAGGACT 
AAGAAGTTGT CATCAAAAAA TTTAAAGAAT CCTTATGAAG AGCCAAGCAG AGAGGGTAGC 
AGTAGCAGGC CTTCAGACAG CGAGGCTAGG ATGGCCTCTT TTCCCCAACC TTGGTTTGTG 
GTTATAAAGG AACATCAAAA TCTCAGGAAT GGAAGTCAAC CTATGGCCCT CAAGGATGGT 
GAGTCAGCAG ATGTTAGCCA ATTTTCCTCT CCAGAGCCAG GGTCAGTAAA ACGGACCTCA 
TGGAGAAATG AACACCAGTT ATATAAGGAC ACAGAGCAAG GCTGCTGCAC TCCACCATCC 
AGTAATAGAG GCACCGGCTC TCAGTCAATG GAGCAGAGCT TTTCTGTCCC CTCCTATGAG 
GCACAGCCCA TTGCTTTGGG GGTTGAGAAG CCACAGTCTC TCCTATCAGC TAAACCCTTA 
TTGCAACAAA GGGCCCCAGA TCCACCACAC CAAATGAAGC TGATTCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( XM_022413384.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022269092.1 Canis lupus familiaris epidermal growth factor (EGF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe284002
Clone ID Related Accession (Same CDS sequence)	XM_022413384.1
Accession Version	XM_022413384.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	pro-epidermal growth factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCC CCCTTATCAT TCTGTGGCCG GTAGTTTTTA AATGTAGTTT TGCTAGTCTC 
TCAGACCCGG AGAACTGGAA CTGTCCTGAA GTCTCTCCCT CAGGAAAGGG GAGCCCTGCT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA TCAGTATCTT TAGGATTGAC 
CTGGAAGGCA CTAATCATGA GCAATTGGTG GCAGATGCTG GTGTATCAGT GATCATGGAT 
TTTCATTATA ATAAAGAAAG AATCTATTGG GTAGATCCAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGACAAG GCAAGAGAGA GTATGCAATA TAGAGAAAAA TGTTTCAGGA 
ATGGCAATAA ATTGGATAAA TGAAGAACTT ATTTGGTCAA ATCAACAGGA AGGAATCATC 
ACAGTAACAG ATATGAAAGG AAACAATTCG CGAGTTCTCT TAAGAGCCTT AAACTATCCT 
GCAAATGTAG CAATTGATCC AATAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCAGTG 
GCTGGCAGCC TTCACAGAGC AGATCTCAAT GGTGTGGAAG AGAAGATTCT TTTACAGACA 
TCAGAAAGAA TAACAGCTGT GTCATTGGAT GTGCTTGATA AACAGCTTTT TTGGATTCAG 
TACAGCAGAG ATGGAAGCAA TTCTCATATT TATTCCTGTA ATTATGATGG AGGTTCTGTC 
CATCTTAGCA AACATCTTAC ACAGCATAAT TTTTTTGCAA TGTCCCTTTT TGGGAATCAG 
ATCTTCTATT CAACATGGAA AAAGAAGACA ATTTGGATAG CTAACAAACA CAGTGGGAAG 
GATATGGTTA GAATTAACCT AGATTCATCA TTTGTACCAC CTGGTGGAAT CAAAGTAGTG 
CATCCACTCT TACAGCCCAA GGCAGAGAGT GGCACTTGGT CACCTGATCA GAAACTCTGC 
AAATGGAAGC AAGGTAACTG CAGAGGCAGC ACGTGTGGTC AAGATTCAAA GTCCTATTCA 
TGCACGTGTG CAGAGGGATA TACTTTAAGC CAAGATGGAA AATACTGTGA AGATGTCAAT 
GAATGTGCCT TTTGGAATCA TGGCTGTACT CTTGGGTGTG AAAACATCCC TGGATCTTAT 
TATTGCACAT GCCCTGTAGG ATTTATTCTG CTTCCTGATG GGAAACGGTG TCATCAATTA 
ATTGCCTGTC CGAGCAATAC ATCTAAATGT AGCCATGACT GTGTTCTGAC ATCAGATGGT 
CCCATATGTT TCTGTCCTGA AGGCTCAGTG CTTGAGGCAG ATGGAAAAAC ATGTAGTGGC 
TGTTCATCAC CTGATAATGG TGGATGTAGC CAGCTCTGCC TCCCTCTCAG CCCAGTATCC 
TGGGAATGTG GTTGCTTTCC TGGGTATGAC CTACAACTGG ACAAACAAAG CTGTGCAGCA 
TCAGGACCAC AACCATTTTT GCTGTTTGCC AATTCTCAAG ATATTCGACA CATGCATTTT 
GATGGAACAG ATTATGGAAC CCTGCTCAGC CAGCAAATGG GAATGGTTTT TGCCCTTGAT 
CACGACCCTG TGGAAAATAA GATATACTTT GCCCATACAG CCCTGAAGTG GATAGAGAGA 
GCTAACATGG ATGGTTCCCA GCGAGAAAGG CTTATTGAGG AAGGAGTGGA TGTGCCAGAA 
GGTCTTGCCA TAGATTGGAT TGACCGTAAA TTCTACTGGA CAGACAGCGG AAAATCTCTT 
ATTGAAGGGA GTGATTTAAA TGGAAAACAT CGTGAGGTAA TCATCAAGGA AGACATCTCT 
CAGCCACGAG GAATTGCTGT TCATCCAATG GCCAAGAGAT TATTCTGGAC TGATATGGGG 
ATTAATCCAC GAATTGAAAG TTCTTCCCTT CAAGGCATTG GCCGACTGGT TATAGCTAGC 
TCTGATCTGG TCTGGCCCAG TGGAATAACG ATTGATTATG TAACTGACAA ATTGTACTGG 
TGTGATACCA AGCTGTCTGT GATTGAGATG GCCAATCTGG ATGGTTCAAA ACGCCAAAGA 
CTTGCCCAGA ACGATGTAGG TCACCCATTT GCTATGGCCG TGTTTGAGGA TCACGTGTGG 
TTCTCTGATT GGACTATGCC ATCAATAATA AGAGTGGACA AGAGGACTGG CAAAAACAGG 
GTACGTCTCC GAGGCAGCAT GCTGAAGCCT TCATCACTGG TTGTAGTTCA TCCATTGGCA 
AAACCAGGAG CACAGCCCTG CTTATATCAA AATGGAGGCT GTGAACATAT TTGCAAAGAG 
AGGTTTGGAA CTGCTCAATG TTTGTGTCGT GAAGGTTTTG TGAAAGCCCC AGATGGGAAA 
ATGTGTCTGG CTCTGAATGG CCATCAGATA CCGGCAGATG CTGACGACTG TGCTCCTGTG 
GGATGCAGTA CATGGGCTGA GTGTGTTTCA GAGGGAGAAA ATGCCACATG TCAGTGTTTG 
AAAGGATTTA CTGGGGATGG AAAGCTATGT TTTGACATAG ATGAATGTGA GATGGGCATC 
ACGATTTGCC CTCCTACCTC CTCAAAGTGC GTCAATACTG AAGGTGGTTA TGTTTGTCAG 
TGCTCAGAAG GCTACCGAGG CGATGGGATC CACTGTCTGG ATATTAATGA GTGCCAACTG 
GGCATGCACA CCTGTGGGGA AAATGCCACC TGTACAAATA TGGAGGGAAA CTATACCTGC 
ATGTGCGCTG GCAGCCTGTC TGAACCTGGA CAGATATGTG CTGACTCTAC TCCGCCTTCT 
CATCCCATGG AGGACAGTCA CTATTCTGTG AGAAATGGTT ATCGGGAATG CCCCTCATCC 
TATGACGGGT ACTGCCTCTA TAATGGTGTG TGTATGTACA TTGAAGCAGT CGACAGATAC 
GCATGCAACT GTGTTTTTGG CTACGTCGGG GAGCGATGTC AGCACCGAGA CCTGAAATGG 
GAACTGCGCC ACGCGGGCCA GGGCCGGCAG CGGCAGGTCG CCGCGGTGGC CGTGGGCGTG 
GCCGTGCTCG TCCTGCTGCT GCTGCTCGGG CTGGGGGGCG CGCACTGCTA CAGGACTAAG 
AAGTTGTCAT CAAAAAATTT AAAGAATCCT TATGAAGAGC CAAGCAGAGA GGGTAGCAGT 
AGCAGGCCTT CAGACAGCGA GGCTAGGATG GCCTCTTTTC CCCAACCTTG GTTTGTGGTT 
ATAAAGGAAC ATCAAAATCT CAGGAATGGA AGTCAACCTA TGGCCCTCAA GGATGGTGAG 
TCAGCAGATG TTAGCCGGTT TTCCTCTCCA GAGCCAGGGT CAGTAAAACG GACCTCATGG 
AGAAATGAAC ACCAGTTATA TAAGGACACA GAGCAAGGCT GCTGCACTCC ACCATCCAGT 
AATAGAGGCA CCGGCTCTCA GTCAATGGAG CAGAGCTTTT CTGTCCCCTC CTATGAGGCA 
CAGCCCATTG CTTTGGGGGT TGAGAAGCCA CAGTCTCTCC TATCAGCTAA CCCTTATTGC 
AACAAAGGGC CCCAGATCCA CCACACCAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022269092.1
CDS	539..4051
Translation	MLLPLIILWPVVFKCSFASLSDPENWNCPEVSPSGKGSPACVGPAPFLIFSHGISIFRIDLEGTNHEQLVADAGVSVIMDFHYNKERIYWVDPERQLLQRVFLNGTRQERVCNIEKNVSGMAINWINEELIWSNQQEGIITVTDMKGNNSRVLLRALNYPANVAIDPIERFIFWSSEVAVAGSLHRADLNGVEEKILLQTSERITAVSLDVLDKQLFWIQYSRDGSNSHIYSCNYDGGSVHLSKHLTQHNFFAMSLFGNQIFYSTWKKKTIWIANKHSGKDMVRINLDSSFVPPGGIKVVHPLLQPKAESGTWSPDQKLCKWKQGNCRGSTCGQDSKSYSCTCAEGYTLSQDGKYCEDVNECAFWNHGCTLGCENIPGSYYCTCPVGFILLPDGKRCHQLIACPSNTSKCSHDCVLTSDGPICFCPEGSVLEADGKTCSGCSSPDNGGCSQLCLPLSPVSWECGCFPGYDLQLDKQSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVFALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAIDWIDRKFYWTDSGKSLIEGSDLNGKHREVIIKEDISQPRGIAVHPMAKRLFWTDMGINPRIESSSLQGIGRLVIASSDLVWPSGITIDYVTDKLYWCDTKLSVIEMANLDGSKRQRLAQNDVGHPFAMAVFEDHVWFSDWTMPSIIRVDKRTGKNRVRLRGSMLKPSSLVVVHPLAKPGAQPCLYQNGGCEHICKERFGTAQCLCREGFVKAPDGKMCLALNGHQIPADADDCAPVGCSTWAECVSEGENATCQCLKGFTGDGKLCFDIDECEMGITICPPTSSKCVNTEGGYVCQCSEGYRGDGIHCLDINECQLGMHTCGENATCTNMEGNYTCMCAGSLSEPGQICADSTPPSHPMEDSHYSVRNGYRECPSSYDGYCLYNGVCMYIEAVDRYACNCVFGYVGERCQHRDLKWELRHAGQGRQRQVAAVAVGVAVLVLLLLLGLGGAHCYRTKKLSSKNLKNPYEEPSREGSSSRPSDSEARMASFPQPWFVVIKEHQNLRNGSQPMALKDGESADVSRFSSPEPGSVKRTSWRNEHQLYKDTEQGCCTPPSSNRGTGSQSMEQSFSVPSYEAQPIALGVEKPQSLLSANPYCNKGPQIHHTK

Target ORF information:

RefSeq Version	XM_022413384.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris epidermal growth factor (EGF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022413384.1

ORF Insert Sequence:

ATGCTGCTCC CCCTTATCAT TCTGTGGCCG GTAGTTTTTA AATGTAGTTT TGCTAGTCTC 
TCAGACCCGG AGAACTGGAA CTGTCCTGAA GTCTCTCCCT CAGGAAAGGG GAGCCCTGCT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA TCAGTATCTT TAGGATTGAC 
CTGGAAGGCA CTAATCATGA GCAATTGGTG GCAGATGCTG GTGTATCAGT GATCATGGAT 
TTTCATTATA ATAAAGAAAG AATCTATTGG GTAGATCCAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGACAAG GCAAGAGAGA GTATGCAATA TAGAGAAAAA TGTTTCAGGA 
ATGGCAATAA ATTGGATAAA TGAAGAACTT ATTTGGTCAA ATCAACAGGA AGGAATCATC 
ACAGTAACAG ATATGAAAGG AAACAATTCG CGAGTTCTCT TAAGAGCCTT AAACTATCCT 
GCAAATGTAG CAATTGATCC AATAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCAGTG 
GCTGGCAGCC TTCACAGAGC AGATCTCAAT GGTGTGGAAG AGAAGATTCT TTTACAGACA 
TCAGAAAGAA TAACAGCTGT GTCATTGGAT GTGCTTGATA AACAGCTTTT TTGGATTCAG 
TACAGCAGAG ATGGAAGCAA TTCTCATATT TATTCCTGTA ATTATGATGG AGGTTCTGTC 
CATCTTAGCA AACATCTTAC ACAGCATAAT TTTTTTGCAA TGTCCCTTTT TGGGAATCAG 
ATCTTCTATT CAACATGGAA AAAGAAGACA ATTTGGATAG CTAACAAACA CAGTGGGAAG 
GATATGGTTA GAATTAACCT AGATTCATCA TTTGTACCAC CTGGTGGAAT CAAAGTAGTG 
CATCCACTCT TACAGCCCAA GGCAGAGAGT GGCACTTGGT CACCTGATCA GAAACTCTGC 
AAATGGAAGC AAGGTAACTG CAGAGGCAGC ACGTGTGGTC AAGATTCAAA GTCCTATTCA 
TGCACGTGTG CAGAGGGATA TACTTTAAGC CAAGATGGAA AATACTGTGA AGATGTCAAT 
GAATGTGCCT TTTGGAATCA TGGCTGTACT CTTGGGTGTG AAAACATCCC TGGATCTTAT 
TATTGCACAT GCCCTGTAGG ATTTATTCTG CTTCCTGATG GGAAACGGTG TCATCAATTA 
ATTGCCTGTC CGAGCAATAC ATCTAAATGT AGCCATGACT GTGTTCTGAC ATCAGATGGT 
CCCATATGTT TCTGTCCTGA AGGCTCAGTG CTTGAGGCAG ATGGAAAAAC ATGTAGTGGC 
TGTTCATCAC CTGATAATGG TGGATGTAGC CAGCTCTGCC TCCCTCTCAG CCCAGTATCC 
TGGGAATGTG GTTGCTTTCC TGGGTATGAC CTACAACTGG ACAAACAAAG CTGTGCAGCA 
TCAGGACCAC AACCATTTTT GCTGTTTGCC AATTCTCAAG ATATTCGACA CATGCATTTT 
GATGGAACAG ATTATGGAAC CCTGCTCAGC CAGCAAATGG GAATGGTTTT TGCCCTTGAT 
CACGACCCTG TGGAAAATAA GATATACTTT GCCCATACAG CCCTGAAGTG GATAGAGAGA 
GCTAACATGG ATGGTTCCCA GCGAGAAAGG CTTATTGAGG AAGGAGTGGA TGTGCCAGAA 
GGTCTTGCCA TAGATTGGAT TGACCGTAAA TTCTACTGGA CAGACAGCGG AAAATCTCTT 
ATTGAAGGGA GTGATTTAAA TGGAAAACAT CGTGAGGTAA TCATCAAGGA AGACATCTCT 
CAGCCACGAG GAATTGCTGT TCATCCAATG GCCAAGAGAT TATTCTGGAC TGATATGGGG 
ATTAATCCAC GAATTGAAAG TTCTTCCCTT CAAGGCATTG GCCGACTGGT TATAGCTAGC 
TCTGATCTGG TCTGGCCCAG TGGAATAACG ATTGATTATG TAACTGACAA ATTGTACTGG 
TGTGATACCA AGCTGTCTGT GATTGAGATG GCCAATCTGG ATGGTTCAAA ACGCCAAAGA 
CTTGCCCAGA ACGATGTAGG TCACCCATTT GCTATGGCCG TGTTTGAGGA TCACGTGTGG 
TTCTCTGATT GGACTATGCC ATCAATAATA AGAGTGGACA AGAGGACTGG CAAAAACAGG 
GTACGTCTCC GAGGCAGCAT GCTGAAGCCT TCATCACTGG TTGTAGTTCA TCCATTGGCA 
AAACCAGGAG CACAGCCCTG CTTATATCAA AATGGAGGCT GTGAACATAT TTGCAAAGAG 
AGGTTTGGAA CTGCTCAATG TTTGTGTCGT GAAGGTTTTG TGAAAGCCCC AGATGGGAAA 
ATGTGTCTGG CTCTGAATGG CCATCAGATA CCGGCAGATG CTGACGACTG TGCTCCTGTG 
GGATGCAGTA CATGGGCTGA GTGTGTTTCA GAGGGAGAAA ATGCCACATG TCAGTGTTTG 
AAAGGATTTA CTGGGGATGG AAAGCTATGT TTTGACATAG ATGAATGTGA GATGGGCATC 
ACGATTTGCC CTCCTACCTC CTCAAAGTGC GTCAATACTG AAGGTGGTTA TGTTTGTCAG 
TGCTCAGAAG GCTACCGAGG CGATGGGATC CACTGTCTGG ATATTAATGA GTGCCAACTG 
GGCATGCACA CCTGTGGGGA AAATGCCACC TGTACAAATA TGGAGGGAAA CTATACCTGC 
ATGTGCGCTG GCAGCCTGTC TGAACCTGGA CAGATATGTG CTGACTCTAC TCCGCCTTCT 
CATCCCATGG AGGACAGTCA CTATTCTGTG AGAAATGGTT ATCGGGAATG CCCCTCATCC 
TATGACGGGT ACTGCCTCTA TAATGGTGTG TGTATGTACA TTGAAGCAGT CGACAGATAC 
GCATGCAACT GTGTTTTTGG CTACGTCGGG GAGCGATGTC AGCACCGAGA CCTGAAATGG 
GAACTGCGCC ACGCGGGCCA GGGCCGGCAG CGGCAGGTCG CCGCGGTGGC CGTGGGCGTG 
GCCGTGCTCG TCCTGCTGCT GCTGCTCGGG CTGGGGGGCG CGCACTGCTA CAGGACTAAG 
AAGTTGTCAT CAAAAAATTT AAAGAATCCT TATGAAGAGC CAAGCAGAGA GGGTAGCAGT 
AGCAGGCCTT CAGACAGCGA GGCTAGGATG GCCTCTTTTC CCCAACCTTG GTTTGTGGTT 
ATAAAGGAAC ATCAAAATCT CAGGAATGGA AGTCAACCTA TGGCCCTCAA GGATGGTGAG 
TCAGCAGATG TTAGCCGGTT TTCCTCTCCA GAGCCAGGGT CAGTAAAACG GACCTCATGG 
AGAAATGAAC ACCAGTTATA TAAGGACACA GAGCAAGGCT GCTGCACTCC ACCATCCAGT 
AATAGAGGCA CCGGCTCTCA GTCAATGGAG CAGAGCTTTT CTGTCCCCTC CTATGAGGCA 
CAGCCCATTG CTTTGGGGGT TGAGAAGCCA CAGTCTCTCC TATCAGCTAA CCCTTATTGC 
AACAAAGGGC CCCAGATCCA CCACACCAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( XM_014109884.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013965359.1 Canis lupus familiaris epidermal growth factor (EGF), transcript variant X1, mRNA.

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CloneID	OCe44531
Clone ID Related Accession (Same CDS sequence)	XM_014109884.2 , XM_014109884.1
Accession Version	XM_014109884.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	pro-epidermal growth factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006614.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109884.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCC CCCTTATCAT TCTGTGGCCG GTAGTTTTTA AATGTAGTTT TGCTAGTCTC 
TCAGACCCGG AGAACTGGAA CTGTCCTGAA GTCTCTCCCT CAGGAAAGGG GAGCCCTGCT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA TCAGTATCTT TAGGATTGAC 
CTGGAAGGCA CTAATCATGA GCAATTGGTG GCAGATGCTG GTGTATCAGT GATCATGGAT 
TTTCATTATA ATAAAGAAAG AATCTATTGG GTAGATCCAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGACAAG GCAAGAGAGA GTATGCAATA TAGAGAAAAA TGTTTCAGGA 
ATGGCAATAA ATTGGATAAA TGAAGAACTT ATTTGGTCAA ATCAACAGGA AGGAATCATC 
ACAGTAACAG ATATGAAAGG AAACAATTCG CGAGTTCTCT TAAGAGCCTT AAACTATCCT 
GCAAATGTAG CAATTGATCC AATAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCAGTG 
GCTGGCAGCC TTCACAGAGC AGATCTCAAT GGTGTGGAAG AGAAGATTCT TTTACAGACA 
TCAGAAAGAA TAACAGCTGT GTCATTGGAT GTGCTTGATA AACAGCTTTT TTGGATTCAG 
TACAGCAGAG ATGGAAGCAA TTCTCATATT TATTCCTGTA ATTATGATGG AGGTTCTGTC 
CATCTTAGCA AACATCTTAC ACAGCATAAT TTTTTTGCAA TGTCCCTTTT TGGGAATCAG 
ATCTTCTATT CAACATGGAA AAAGAAGACA ATTTGGATAG CTAACAAACA CAGTGGGAAG 
GATATGGTTA GAATTAACCT AGATTCATCA TTTGTACCAC CTGGTGGAAT CAAAGTAGTG 
CATCCACTCT TACAGCCCAA GGCAGAGAGT GGCACTTGGT CACCTGATCA GAAACTCTGC 
AAATGGAAGC AAGGTAACTG CAGAGGCAGC ACGTGTGGTC AAGATTCAAA GTCCTATTCA 
TGCACGTGTG CAGAGGGATA TACTTTAAGC CAAGATGGAA AATACTGTGA AGATGTCAAT 
GAATGTGCCT TTTGGAATCA TGGCTGTACT CTTGGGTGTG AAAACATCCC TGGATCTTAT 
TATTGCACAT GCCCTGTAGG ATTTATTCTG CTTCCTGATG GGAAACGGTG TCATCAATTA 
ATTGCCTGTC CGAGCAATAC ATCTAAATGT AGCCATGACT GTGTTCTGAC ATCAGATGGT 
CCCATATGTT TCTGTCCTGA AGGCTCAGTG CTTGAGGCAG ATGGAAAAAC ATGTAGTGGC 
TGTTCATCAC CTGATAATGG TGGATGTAGC CAGCTCTGCC TCCCTCTCAG CCCAGTATCC 
TGGGAATGTG GTTGCTTTCC TGGGTATGAC CTACAACTGG ACAAACAAAG CTGTGCAGCA 
TCAGGACCAC AACCATTTTT GCTGTTTGCC AATTCTCAAG ATATTCGACA CATGCATTTT 
GATGGAACAG ATTATGGAAC CCTGCTCAGC CAGCAAATGG GAATGGTTTT TGCCCTTGAT 
CACGACCCTG TGGAAAATAA GATATACTTT GCCCATACAG CCCTGAAGTG GATAGAGAGA 
GCTAACATGG ATGGTTCCCA GCGAGAAAGG CTTATTGAGG AAGGAGTGGA TGTGCCAGAA 
GGTCTTGCCA TAGATTGGAT TGACCGTAAA TTCTACTGGA CAGACAGCGG AAAATCTCTT 
ATTGAAGGGA GTGATTTAAA TGGAAAACAT CGTGAGGTAA TCATCAAGGA AGACATCTCT 
CAGCCACGAG GAATTGCTGT TCATCCAATG GCCAAGAGAT TATTCTGGAC TGATATGGGG 
ATTAATCCAC GAATTGAAAG TTCTTCCCTT CAAGGCATTG GCCGACTGGT TATAGCTAGC 
TCTGATCTGG TCTGGCCCAG TGGAATAACG ATTGATTATG TAACTGACAA ATTGTACTGG 
TGTGATACCA AGCTGTCTGT GATTGAGATG GCCAATCTGG ATGGTTCAAA ACGCCAAAGA 
CTTGCCCAGA ACGATGTAGG TCACCCATTT GCTATGGCCG TGTTTGAGGA TCACGTGTGG 
TTCTCTGATT GGACTATGCC ATCAATAATA AGAGTGGACA AGAGGACTGG CAAAAACAGG 
GTACGTCTCC GAGGCAGCAT GCTGAAGCCT TCATCACTGG TTGTAGTTCA TCCATTGGCA 
AAACCAGGAG CACAGCCCTG CTTATATCAA AATGGAGGCT GTGAACATAT TTGCAAAGAG 
AGGTTTGGAA CTGCTCAATG TTTGTGTCGT GAAGGTTTTG TGAAAGCCCC AGATGGGAAA 
ATGTGTCTGG CTCTGAATGG CCATCAGATA CCGGCAGTAG GTAGTGAAGC AGATCTAAGT 
AATCACGTAA CGCCAGGGGA TGTCTTACCC AGAAGTGAAG GATTTGAAGA TAACATTACA 
GAATCTCAGC ATATGCTAGT GGCCGAAATC ATGGTGTCAG ATGCTGACGA CTGTGCTCCT 
GTGGGATGCA GTACATGGGC TGAGTGTGTT TCAGAGGGAG AAAATGCCAC ATGTCAGTGT 
TTGAAAGGAT TTACTGGGGA TGGAAAGCTA TGTTTTGACA TAGATGAATG TGAGATGGGC 
ATCACGATTT GCCCTCCTAC CTCCTCAAAG TGCGTCAATA CTGAAGGTGG TTATGTTTGT 
CAGTGCTCAG AAGGCTACCG AGGCGATGGG ATCCACTGTC TGGATATTAA TGAGTGCCAA 
CTGGGCATGC ACACCTGTGG GGAAAATGCC ACCTGTACAA ATATGGAGGG AAACTATACC 
TGCATGTGCG CTGGCAGCCT GTCTGAACCT GGACAGATAT GTGCTGACTC TACTCCGCCT 
TCTCATCCCA TGGAGGACAG TCACTATTCT GTGAGAAATG GTTATCGGGA ATGCCCCTCA 
TCCTATGACG GGTACTGCCT CTATAATGGT GTGTGTATGT ACATTGAAGC AGTCGACAGA 
TACGCATGCA ACTGTGTTTT TGGCTACGTC GGGGAGCGAT GTCAGCACCG AGACCTGAAA 
TGGGAACTGC GCCACGCGGG CCAGGGCCGG CAGCGGCAGG TCGCCGCGGT GGCCGTGGGC 
GTGGCCGTGC TCGTCCTGCT GCTGCTGCTC GGGCTGGGGG GCGCGCACTG CTACAGGACT 
AAGAAGTTGT CATCAAAAAA TTTAAAGAAT CCTTATGAAG AGCCAAGCAG AGAGGGTAGC 
AGTAGCAGGC CTTCAGACAG CGAGGCTAGG ATGGCCTCTT TTCCCCAACC TTGGTTTGTG 
GTTATAAAGG AACATCAAAA TCTCAGGAAT GGAAGTCAAC CTATGGCCCT CAAGGATGGT 
GAGTCAGCAG ATGTTAGCCG GTTTTCCTCT CCAGAGCCAG GGTCAGTAAA ACGGACCTCA 
TGGAGAAATG AACACCAGTT ATATAAGGAC ACAGAGCAAG GCTGCTGCAC TCCACCATCC 
AGTAATAGAG GCACCGGCTC TCAGTCAATG GAGCAGAGCT TTTCTGTCCC CTCCTATGAG 
GCACAGCCCA TTGCTTTGGG GGTTGAGAAG CCACAGTCTC TCCTATCAGC TAACCCTTAT 
TGCAACAAAG GGCCCCAGAT CCACCACACC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965359.1
CDS	539..4174
Translation	MLLPLIILWPVVFKCSFASLSDPENWNCPEVSPSGKGSPACVGPAPFLIFSHGISIFRIDLEGTNHEQLVADAGVSVIMDFHYNKERIYWVDPERQLLQRVFLNGTRQERVCNIEKNVSGMAINWINEELIWSNQQEGIITVTDMKGNNSRVLLRALNYPANVAIDPIERFIFWSSEVAVAGSLHRADLNGVEEKILLQTSERITAVSLDVLDKQLFWIQYSRDGSNSHIYSCNYDGGSVHLSKHLTQHNFFAMSLFGNQIFYSTWKKKTIWIANKHSGKDMVRINLDSSFVPPGGIKVVHPLLQPKAESGTWSPDQKLCKWKQGNCRGSTCGQDSKSYSCTCAEGYTLSQDGKYCEDVNECAFWNHGCTLGCENIPGSYYCTCPVGFILLPDGKRCHQLIACPSNTSKCSHDCVLTSDGPICFCPEGSVLEADGKTCSGCSSPDNGGCSQLCLPLSPVSWECGCFPGYDLQLDKQSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVFALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAIDWIDRKFYWTDSGKSLIEGSDLNGKHREVIIKEDISQPRGIAVHPMAKRLFWTDMGINPRIESSSLQGIGRLVIASSDLVWPSGITIDYVTDKLYWCDTKLSVIEMANLDGSKRQRLAQNDVGHPFAMAVFEDHVWFSDWTMPSIIRVDKRTGKNRVRLRGSMLKPSSLVVVHPLAKPGAQPCLYQNGGCEHICKERFGTAQCLCREGFVKAPDGKMCLALNGHQIPAVGSEADLSNHVTPGDVLPRSEGFEDNITESQHMLVAEIMVSDADDCAPVGCSTWAECVSEGENATCQCLKGFTGDGKLCFDIDECEMGITICPPTSSKCVNTEGGYVCQCSEGYRGDGIHCLDINECQLGMHTCGENATCTNMEGNYTCMCAGSLSEPGQICADSTPPSHPMEDSHYSVRNGYRECPSSYDGYCLYNGVCMYIEAVDRYACNCVFGYVGERCQHRDLKWELRHAGQGRQRQVAAVAVGVAVLVLLLLLGLGGAHCYRTKKLSSKNLKNPYEEPSREGSSSRPSDSEARMASFPQPWFVVIKEHQNLRNGSQPMALKDGESADVSRFSSPEPGSVKRTSWRNEHQLYKDTEQGCCTPPSSNRGTGSQSMEQSFSVPSYEAQPIALGVEKPQSLLSANPYCNKGPQIHHTK

Target ORF information:

RefSeq Version	XM_014109884.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris epidermal growth factor (EGF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109884.2

ORF Insert Sequence:

ATGCTGCTCC CCCTTATCAT TCTGTGGCCG GTAGTTTTTA AATGTAGTTT TGCTAGTCTC 
TCAGACCCGG AGAACTGGAA CTGTCCTGAA GTCTCTCCCT CAGGAAAGGG GAGCCCTGCT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA TCAGTATCTT TAGGATTGAC 
CTGGAAGGCA CTAATCATGA GCAATTGGTG GCAGATGCTG GTGTATCAGT GATCATGGAT 
TTTCATTATA ATAAAGAAAG AATCTATTGG GTAGATCCAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGACAAG GCAAGAGAGA GTATGCAATA TAGAGAAAAA TGTTTCAGGA 
ATGGCAATAA ATTGGATAAA TGAAGAACTT ATTTGGTCAA ATCAACAGGA AGGAATCATC 
ACAGTAACAG ATATGAAAGG AAACAATTCG CGAGTTCTCT TAAGAGCCTT AAACTATCCT 
GCAAATGTAG CAATTGATCC AATAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCAGTG 
GCTGGCAGCC TTCACAGAGC AGATCTCAAT GGTGTGGAAG AGAAGATTCT TTTACAGACA 
TCAGAAAGAA TAACAGCTGT GTCATTGGAT GTGCTTGATA AACAGCTTTT TTGGATTCAG 
TACAGCAGAG ATGGAAGCAA TTCTCATATT TATTCCTGTA ATTATGATGG AGGTTCTGTC 
CATCTTAGCA AACATCTTAC ACAGCATAAT TTTTTTGCAA TGTCCCTTTT TGGGAATCAG 
ATCTTCTATT CAACATGGAA AAAGAAGACA ATTTGGATAG CTAACAAACA CAGTGGGAAG 
GATATGGTTA GAATTAACCT AGATTCATCA TTTGTACCAC CTGGTGGAAT CAAAGTAGTG 
CATCCACTCT TACAGCCCAA GGCAGAGAGT GGCACTTGGT CACCTGATCA GAAACTCTGC 
AAATGGAAGC AAGGTAACTG CAGAGGCAGC ACGTGTGGTC AAGATTCAAA GTCCTATTCA 
TGCACGTGTG CAGAGGGATA TACTTTAAGC CAAGATGGAA AATACTGTGA AGATGTCAAT 
GAATGTGCCT TTTGGAATCA TGGCTGTACT CTTGGGTGTG AAAACATCCC TGGATCTTAT 
TATTGCACAT GCCCTGTAGG ATTTATTCTG CTTCCTGATG GGAAACGGTG TCATCAATTA 
ATTGCCTGTC CGAGCAATAC ATCTAAATGT AGCCATGACT GTGTTCTGAC ATCAGATGGT 
CCCATATGTT TCTGTCCTGA AGGCTCAGTG CTTGAGGCAG ATGGAAAAAC ATGTAGTGGC 
TGTTCATCAC CTGATAATGG TGGATGTAGC CAGCTCTGCC TCCCTCTCAG CCCAGTATCC 
TGGGAATGTG GTTGCTTTCC TGGGTATGAC CTACAACTGG ACAAACAAAG CTGTGCAGCA 
TCAGGACCAC AACCATTTTT GCTGTTTGCC AATTCTCAAG ATATTCGACA CATGCATTTT 
GATGGAACAG ATTATGGAAC CCTGCTCAGC CAGCAAATGG GAATGGTTTT TGCCCTTGAT 
CACGACCCTG TGGAAAATAA GATATACTTT GCCCATACAG CCCTGAAGTG GATAGAGAGA 
GCTAACATGG ATGGTTCCCA GCGAGAAAGG CTTATTGAGG AAGGAGTGGA TGTGCCAGAA 
GGTCTTGCCA TAGATTGGAT TGACCGTAAA TTCTACTGGA CAGACAGCGG AAAATCTCTT 
ATTGAAGGGA GTGATTTAAA TGGAAAACAT CGTGAGGTAA TCATCAAGGA AGACATCTCT 
CAGCCACGAG GAATTGCTGT TCATCCAATG GCCAAGAGAT TATTCTGGAC TGATATGGGG 
ATTAATCCAC GAATTGAAAG TTCTTCCCTT CAAGGCATTG GCCGACTGGT TATAGCTAGC 
TCTGATCTGG TCTGGCCCAG TGGAATAACG ATTGATTATG TAACTGACAA ATTGTACTGG 
TGTGATACCA AGCTGTCTGT GATTGAGATG GCCAATCTGG ATGGTTCAAA ACGCCAAAGA 
CTTGCCCAGA ACGATGTAGG TCACCCATTT GCTATGGCCG TGTTTGAGGA TCACGTGTGG 
TTCTCTGATT GGACTATGCC ATCAATAATA AGAGTGGACA AGAGGACTGG CAAAAACAGG 
GTACGTCTCC GAGGCAGCAT GCTGAAGCCT TCATCACTGG TTGTAGTTCA TCCATTGGCA 
AAACCAGGAG CACAGCCCTG CTTATATCAA AATGGAGGCT GTGAACATAT TTGCAAAGAG 
AGGTTTGGAA CTGCTCAATG TTTGTGTCGT GAAGGTTTTG TGAAAGCCCC AGATGGGAAA 
ATGTGTCTGG CTCTGAATGG CCATCAGATA CCGGCAGTAG GTAGTGAAGC AGATCTAAGT 
AATCACGTAA CGCCAGGGGA TGTCTTACCC AGAAGTGAAG GATTTGAAGA TAACATTACA 
GAATCTCAGC ATATGCTAGT GGCCGAAATC ATGGTGTCAG ATGCTGACGA CTGTGCTCCT 
GTGGGATGCA GTACATGGGC TGAGTGTGTT TCAGAGGGAG AAAATGCCAC ATGTCAGTGT 
TTGAAAGGAT TTACTGGGGA TGGAAAGCTA TGTTTTGACA TAGATGAATG TGAGATGGGC 
ATCACGATTT GCCCTCCTAC CTCCTCAAAG TGCGTCAATA CTGAAGGTGG TTATGTTTGT 
CAGTGCTCAG AAGGCTACCG AGGCGATGGG ATCCACTGTC TGGATATTAA TGAGTGCCAA 
CTGGGCATGC ACACCTGTGG GGAAAATGCC ACCTGTACAA ATATGGAGGG AAACTATACC 
TGCATGTGCG CTGGCAGCCT GTCTGAACCT GGACAGATAT GTGCTGACTC TACTCCGCCT 
TCTCATCCCA TGGAGGACAG TCACTATTCT GTGAGAAATG GTTATCGGGA ATGCCCCTCA 
TCCTATGACG GGTACTGCCT CTATAATGGT GTGTGTATGT ACATTGAAGC AGTCGACAGA 
TACGCATGCA ACTGTGTTTT TGGCTACGTC GGGGAGCGAT GTCAGCACCG AGACCTGAAA 
TGGGAACTGC GCCACGCGGG CCAGGGCCGG CAGCGGCAGG TCGCCGCGGT GGCCGTGGGC 
GTGGCCGTGC TCGTCCTGCT GCTGCTGCTC GGGCTGGGGG GCGCGCACTG CTACAGGACT 
AAGAAGTTGT CATCAAAAAA TTTAAAGAAT CCTTATGAAG AGCCAAGCAG AGAGGGTAGC 
AGTAGCAGGC CTTCAGACAG CGAGGCTAGG ATGGCCTCTT TTCCCCAACC TTGGTTTGTG 
GTTATAAAGG AACATCAAAA TCTCAGGAAT GGAAGTCAAC CTATGGCCCT CAAGGATGGT 
GAGTCAGCAG ATGTTAGCCG GTTTTCCTCT CCAGAGCCAG GGTCAGTAAA ACGGACCTCA 
TGGAGAAATG AACACCAGTT ATATAAGGAC ACAGAGCAAG GCTGCTGCAC TCCACCATCC 
AGTAATAGAG GCACCGGCTC TCAGTCAATG GAGCAGAGCT TTTCTGTCCC CTCCTATGAG 
GCACAGCCCA TTGCTTTGGG GGTTGAGAAG CCACAGTCTC TCCTATCAGC TAACCCTTAT 
TGCAACAAAG GGCCCCAGAT CCACCACACC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EGF ( XM_014109884.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013965359.1 Canis lupus familiaris epidermal growth factor (EGF), transcript variant X1, mRNA.

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CloneID	OCe44531
Clone ID Related Accession (Same CDS sequence)	XM_014109884.2 , XM_014109884.1
Accession Version	XM_014109884.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	pro-epidermal growth factor isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726116.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCC CCCTTATCAT TCTGTGGCCG GTAGTTTTTA AATGTAGTTT TGCTAGTCTC 
TCAGACCCGG AGAACTGGAA CTGTCCTGAA GTCTCTCCCT CAGGAAAGGG GAGCCCTGCT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA TCAGTATCTT TAGGATTGAC 
CTGGAAGGCA CTAATCATGA GCAATTGGTG GCAGATGCTG GTGTATCAGT GATCATGGAT 
TTTCATTATA ATAAAGAAAG AATCTATTGG GTAGATCCAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGACAAG GCAAGAGAGA GTATGCAATA TAGAGAAAAA TGTTTCAGGA 
ATGGCAATAA ATTGGATAAA TGAAGAACTT ATTTGGTCAA ATCAACAGGA AGGAATCATC 
ACAGTAACAG ATATGAAAGG AAACAATTCG CGAGTTCTCT TAAGAGCCTT AAACTATCCT 
GCAAATGTAG CAATTGATCC AATAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCAGTG 
GCTGGCAGCC TTCACAGAGC AGATCTCAAT GGTGTGGAAG AGAAGATTCT TTTACAGACA 
TCAGAAAGAA TAACAGCTGT GTCATTGGAT GTGCTTGATA AACAGCTTTT TTGGATTCAG 
TACAGCAGAG ATGGAAGCAA TTCTCATATT TATTCCTGTA ATTATGATGG AGGTTCTGTC 
CATCTTAGCA AACATCTTAC ACAGCATAAT TTTTTTGCAA TGTCCCTTTT TGGGAATCAG 
ATCTTCTATT CAACATGGAA AAAGAAGACA ATTTGGATAG CTAACAAACA CAGTGGGAAG 
GATATGGTTA GAATTAACCT AGATTCATCA TTTGTACCAC CTGGTGGAAT CAAAGTAGTG 
CATCCACTCT TACAGCCCAA GGCAGAGAGT GGCACTTGGT CACCTGATCA GAAACTCTGC 
AAATGGAAGC AAGGTAACTG CAGAGGCAGC ACGTGTGGTC AAGATTCAAA GTCCTATTCA 
TGCACGTGTG CAGAGGGATA TACTTTAAGC CAAGATGGAA AATACTGTGA AGATGTCAAT 
GAATGTGCCT TTTGGAATCA TGGCTGTACT CTTGGGTGTG AAAACATCCC TGGATCTTAT 
TATTGCACAT GCCCTGTAGG ATTTATTCTG CTTCCTGATG GGAAACGGTG TCATCAATTA 
ATTGCCTGTC CGAGCAATAC ATCTAAATGT AGCCATGACT GTGTTCTGAC ATCAGATGGT 
CCCATATGTT TCTGTCCTGA AGGCTCAGTG CTTGAGGCAG ATGGAAAAAC ATGTAGTGGC 
TGTTCATCAC CTGATAATGG TGGATGTAGC CAGCTCTGCC TCCCTCTCAG CCCAGTATCC 
TGGGAATGTG GTTGCTTTCC TGGGTATGAC CTACAACTGG ACAAACAAAG CTGTGCAGCA 
TCAGGACCAC AACCATTTTT GCTGTTTGCC AATTCTCAAG ATATTCGACA CATGCATTTT 
GATGGAACAG ATTATGGAAC CCTGCTCAGC CAGCAAATGG GAATGGTTTT TGCCCTTGAT 
CACGACCCTG TGGAAAATAA GATATACTTT GCCCATACAG CCCTGAAGTG GATAGAGAGA 
GCTAACATGG ATGGTTCCCA GCGAGAAAGG CTTATTGAGG AAGGAGTGGA TGTGCCAGAA 
GGTCTTGCCA TAGATTGGAT TGACCGTAAA TTCTACTGGA CAGACAGCGG AAAATCTCTT 
ATTGAAGGGA GTGATTTAAA TGGAAAACAT CGTGAGGTAA TCATCAAGGA AGACATCTCT 
CAGCCACGAG GAATTGCTGT TCATCCAATG GCCAAGAGAT TATTCTGGAC TGATATGGGG 
ATTAATCCAC GAATTGAAAG TTCTTCCCTT CAAGGCATTG GCCGACTGGT TATAGCTAGC 
TCTGATCTGG TCTGGCCCAG TGGAATAACG ATTGATTATG TAACTGACAA ATTGTACTGG 
TGTGATACCA AGCTGTCTGT GATTGAGATG GCCAATCTGG ATGGTTCAAA ACGCCAAAGA 
CTTGCCCAGA ACGATGTAGG TCACCCATTT GCTATGGCCG TGTTTGAGGA TCACGTGTGG 
TTCTCTGATT GGACTATGCC ATCAATAATA AGAGTGGACA AGAGGACTGG CAAAAACAGG 
GTACGTCTCC GAGGCAGCAT GCTGAAGCCT TCATCACTGG TTGTAGTTCA TCCATTGGCA 
AAACCAGGAG CACAGCCCTG CTTATATCAA AATGGAGGCT GTGAACATAT TTGCAAAGAG 
AGGTTTGGAA CTGCTCAATG TTTGTGTCGT GAAGGTTTTG TGAAAGCCCC AGATGGGAAA 
ATGTGTCTGG CTCTGAATGG CCATCAGATA CCGGCAGTAG GTAGTGAAGC AGATCTAAGT 
AATCACGTAA CGCCAGGGGA TGTCTTACCC AGAAGTGAAG GATTTGAAGA TAACATTACA 
GAATCTCAGC ATATGCTAGT GGCCGAAATC ATGGTGTCAG ATGCTGACGA CTGTGCTCCT 
GTGGGATGCA GTACATGGGC TGAGTGTGTT TCAGAGGGAG AAAATGCCAC ATGTCAGTGT 
TTGAAAGGAT TTACTGGGGA TGGAAAGCTA TGTTTTGACA TAGATGAATG TGAGATGGGC 
ATCACGATTT GCCCTCCTAC CTCCTCAAAG TGCGTCAATA CTGAAGGTGG TTATGTTTGT 
CAGTGCTCAG AAGGCTACCG AGGCGATGGG ATCCACTGTC TGGATATTAA TGAGTGCCAA 
CTGGGCATGC ACACCTGTGG GGAAAATGCC ACCTGTACAA ATATGGAGGG AAACTATACC 
TGCATGTGCG CTGGCAGCCT GTCTGAACCT GGACAGATAT GTGCTGACTC TACTCCGCCT 
TCTCATCCCA TGGAGGACAG TCACTATTCT GTGAGAAATG GTTATCGGGA ATGCCCCTCA 
TCCTATGACG GGTACTGCCT CTATAATGGT GTGTGTATGT ACATTGAAGC AGTCGACAGA 
TACGCATGCA ACTGTGTTTT TGGCTACGTC GGGGAGCGAT GTCAGCACCG AGACCTGAAA 
TGGGAACTGC GCCACGCGGG CCAGGGCCGG CAGCGGCAGG TCGCCGCGGT GGCCGTGGGC 
GTGGCCGTGC TCGTCCTGCT GCTGCTGCTC GGGCTGGGGG GCGCGCACTG CTACAGGACT 
AAGAAGTTGT CATCAAAAAA TTTAAAGAAT CCTTATGAAG AGCCAAGCAG AGAGGGTAGC 
AGTAGCAGGC CTTCAGACAG CGAGGCTAGG ATGGCCTCTT TTCCCCAACC TTGGTTTGTG 
GTTATAAAGG AACATCAAAA TCTCAGGAAT GGAAGTCAAC CTATGGCCCT CAAGGATGGT 
GAGTCAGCAG ATGTTAGCCG GTTTTCCTCT CCAGAGCCAG GGTCAGTAAA ACGGACCTCA 
TGGAGAAATG AACACCAGTT ATATAAGGAC ACAGAGCAAG GCTGCTGCAC TCCACCATCC 
AGTAATAGAG GCACCGGCTC TCAGTCAATG GAGCAGAGCT TTTCTGTCCC CTCCTATGAG 
GCACAGCCCA TTGCTTTGGG GGTTGAGAAG CCACAGTCTC TCCTATCAGC TAACCCTTAT 
TGCAACAAAG GGCCCCAGAT CCACCACACC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965359.1
CDS	531..4166
Translation	MLLPLIILWPVVFKCSFASLSDPENWNCPEVSPSGKGSPACVGPAPFLIFSHGISIFRIDLEGTNHEQLVADAGVSVIMDFHYNKERIYWVDPERQLLQRVFLNGTRQERVCNIEKNVSGMAINWINEELIWSNQQEGIITVTDMKGNNSRVLLRALNYPANVAIDPIERFIFWSSEVAVAGSLHRADLNGVEEKILLQTSERITAVSLDVLDKQLFWIQYSRDGSNSHIYSCNYDGGSVHLSKHLTQHNFFAMSLFGNQIFYSTWKKKTIWIANKHSGKDMVRINLDSSFVPPGGIKVVHPLLQPKAESGTWSPDQKLCKWKQGNCRGSTCGQDSKSYSCTCAEGYTLSQDGKYCEDVNECAFWNHGCTLGCENIPGSYYCTCPVGFILLPDGKRCHQLIACPSNTSKCSHDCVLTSDGPICFCPEGSVLEADGKTCSGCSSPDNGGCSQLCLPLSPVSWECGCFPGYDLQLDKQSCAASGPQPFLLFANSQDIRHMHFDGTDYGTLLSQQMGMVFALDHDPVENKIYFAHTALKWIERANMDGSQRERLIEEGVDVPEGLAIDWIDRKFYWTDSGKSLIEGSDLNGKHREVIIKEDISQPRGIAVHPMAKRLFWTDMGINPRIESSSLQGIGRLVIASSDLVWPSGITIDYVTDKLYWCDTKLSVIEMANLDGSKRQRLAQNDVGHPFAMAVFEDHVWFSDWTMPSIIRVDKRTGKNRVRLRGSMLKPSSLVVVHPLAKPGAQPCLYQNGGCEHICKERFGTAQCLCREGFVKAPDGKMCLALNGHQIPAVGSEADLSNHVTPGDVLPRSEGFEDNITESQHMLVAEIMVSDADDCAPVGCSTWAECVSEGENATCQCLKGFTGDGKLCFDIDECEMGITICPPTSSKCVNTEGGYVCQCSEGYRGDGIHCLDINECQLGMHTCGENATCTNMEGNYTCMCAGSLSEPGQICADSTPPSHPMEDSHYSVRNGYRECPSSYDGYCLYNGVCMYIEAVDRYACNCVFGYVGERCQHRDLKWELRHAGQGRQRQVAAVAVGVAVLVLLLLLGLGGAHCYRTKKLSSKNLKNPYEEPSREGSSSRPSDSEARMASFPQPWFVVIKEHQNLRNGSQPMALKDGESADVSRFSSPEPGSVKRTSWRNEHQLYKDTEQGCCTPPSSNRGTGSQSMEQSFSVPSYEAQPIALGVEKPQSLLSANPYCNKGPQIHHTK

Target ORF information:

RefSeq Version	XM_014109884.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris epidermal growth factor (EGF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109884.1

ORF Insert Sequence:

ATGCTGCTCC CCCTTATCAT TCTGTGGCCG GTAGTTTTTA AATGTAGTTT TGCTAGTCTC 
TCAGACCCGG AGAACTGGAA CTGTCCTGAA GTCTCTCCCT CAGGAAAGGG GAGCCCTGCT 
TGTGTGGGTC CTGCACCCTT CTTAATTTTC TCCCATGGAA TCAGTATCTT TAGGATTGAC 
CTGGAAGGCA CTAATCATGA GCAATTGGTG GCAGATGCTG GTGTATCAGT GATCATGGAT 
TTTCATTATA ATAAAGAAAG AATCTATTGG GTAGATCCAG AAAGACAACT TTTGCAAAGA 
GTTTTTCTGA ATGGGACAAG GCAAGAGAGA GTATGCAATA TAGAGAAAAA TGTTTCAGGA 
ATGGCAATAA ATTGGATAAA TGAAGAACTT ATTTGGTCAA ATCAACAGGA AGGAATCATC 
ACAGTAACAG ATATGAAAGG AAACAATTCG CGAGTTCTCT TAAGAGCCTT AAACTATCCT 
GCAAATGTAG CAATTGATCC AATAGAAAGG TTTATATTTT GGTCTTCAGA GGTGGCAGTG 
GCTGGCAGCC TTCACAGAGC AGATCTCAAT GGTGTGGAAG AGAAGATTCT TTTACAGACA 
TCAGAAAGAA TAACAGCTGT GTCATTGGAT GTGCTTGATA AACAGCTTTT TTGGATTCAG 
TACAGCAGAG ATGGAAGCAA TTCTCATATT TATTCCTGTA ATTATGATGG AGGTTCTGTC 
CATCTTAGCA AACATCTTAC ACAGCATAAT TTTTTTGCAA TGTCCCTTTT TGGGAATCAG 
ATCTTCTATT CAACATGGAA AAAGAAGACA ATTTGGATAG CTAACAAACA CAGTGGGAAG 
GATATGGTTA GAATTAACCT AGATTCATCA TTTGTACCAC CTGGTGGAAT CAAAGTAGTG 
CATCCACTCT TACAGCCCAA GGCAGAGAGT GGCACTTGGT CACCTGATCA GAAACTCTGC 
AAATGGAAGC AAGGTAACTG CAGAGGCAGC ACGTGTGGTC AAGATTCAAA GTCCTATTCA 
TGCACGTGTG CAGAGGGATA TACTTTAAGC CAAGATGGAA AATACTGTGA AGATGTCAAT 
GAATGTGCCT TTTGGAATCA TGGCTGTACT CTTGGGTGTG AAAACATCCC TGGATCTTAT 
TATTGCACAT GCCCTGTAGG ATTTATTCTG CTTCCTGATG GGAAACGGTG TCATCAATTA 
ATTGCCTGTC CGAGCAATAC ATCTAAATGT AGCCATGACT GTGTTCTGAC ATCAGATGGT 
CCCATATGTT TCTGTCCTGA AGGCTCAGTG CTTGAGGCAG ATGGAAAAAC ATGTAGTGGC 
TGTTCATCAC CTGATAATGG TGGATGTAGC CAGCTCTGCC TCCCTCTCAG CCCAGTATCC 
TGGGAATGTG GTTGCTTTCC TGGGTATGAC CTACAACTGG ACAAACAAAG CTGTGCAGCA 
TCAGGACCAC AACCATTTTT GCTGTTTGCC AATTCTCAAG ATATTCGACA CATGCATTTT 
GATGGAACAG ATTATGGAAC CCTGCTCAGC CAGCAAATGG GAATGGTTTT TGCCCTTGAT 
CACGACCCTG TGGAAAATAA GATATACTTT GCCCATACAG CCCTGAAGTG GATAGAGAGA 
GCTAACATGG ATGGTTCCCA GCGAGAAAGG CTTATTGAGG AAGGAGTGGA TGTGCCAGAA 
GGTCTTGCCA TAGATTGGAT TGACCGTAAA TTCTACTGGA CAGACAGCGG AAAATCTCTT 
ATTGAAGGGA GTGATTTAAA TGGAAAACAT CGTGAGGTAA TCATCAAGGA AGACATCTCT 
CAGCCACGAG GAATTGCTGT TCATCCAATG GCCAAGAGAT TATTCTGGAC TGATATGGGG 
ATTAATCCAC GAATTGAAAG TTCTTCCCTT CAAGGCATTG GCCGACTGGT TATAGCTAGC 
TCTGATCTGG TCTGGCCCAG TGGAATAACG ATTGATTATG TAACTGACAA ATTGTACTGG 
TGTGATACCA AGCTGTCTGT GATTGAGATG GCCAATCTGG ATGGTTCAAA ACGCCAAAGA 
CTTGCCCAGA ACGATGTAGG TCACCCATTT GCTATGGCCG TGTTTGAGGA TCACGTGTGG 
TTCTCTGATT GGACTATGCC ATCAATAATA AGAGTGGACA AGAGGACTGG CAAAAACAGG 
GTACGTCTCC GAGGCAGCAT GCTGAAGCCT TCATCACTGG TTGTAGTTCA TCCATTGGCA 
AAACCAGGAG CACAGCCCTG CTTATATCAA AATGGAGGCT GTGAACATAT TTGCAAAGAG 
AGGTTTGGAA CTGCTCAATG TTTGTGTCGT GAAGGTTTTG TGAAAGCCCC AGATGGGAAA 
ATGTGTCTGG CTCTGAATGG CCATCAGATA CCGGCAGTAG GTAGTGAAGC AGATCTAAGT 
AATCACGTAA CGCCAGGGGA TGTCTTACCC AGAAGTGAAG GATTTGAAGA TAACATTACA 
GAATCTCAGC ATATGCTAGT GGCCGAAATC ATGGTGTCAG ATGCTGACGA CTGTGCTCCT 
GTGGGATGCA GTACATGGGC TGAGTGTGTT TCAGAGGGAG AAAATGCCAC ATGTCAGTGT 
TTGAAAGGAT TTACTGGGGA TGGAAAGCTA TGTTTTGACA TAGATGAATG TGAGATGGGC 
ATCACGATTT GCCCTCCTAC CTCCTCAAAG TGCGTCAATA CTGAAGGTGG TTATGTTTGT 
CAGTGCTCAG AAGGCTACCG AGGCGATGGG ATCCACTGTC TGGATATTAA TGAGTGCCAA 
CTGGGCATGC ACACCTGTGG GGAAAATGCC ACCTGTACAA ATATGGAGGG AAACTATACC 
TGCATGTGCG CTGGCAGCCT GTCTGAACCT GGACAGATAT GTGCTGACTC TACTCCGCCT 
TCTCATCCCA TGGAGGACAG TCACTATTCT GTGAGAAATG GTTATCGGGA ATGCCCCTCA 
TCCTATGACG GGTACTGCCT CTATAATGGT GTGTGTATGT ACATTGAAGC AGTCGACAGA 
TACGCATGCA ACTGTGTTTT TGGCTACGTC GGGGAGCGAT GTCAGCACCG AGACCTGAAA 
TGGGAACTGC GCCACGCGGG CCAGGGCCGG CAGCGGCAGG TCGCCGCGGT GGCCGTGGGC 
GTGGCCGTGC TCGTCCTGCT GCTGCTGCTC GGGCTGGGGG GCGCGCACTG CTACAGGACT 
AAGAAGTTGT CATCAAAAAA TTTAAAGAAT CCTTATGAAG AGCCAAGCAG AGAGGGTAGC 
AGTAGCAGGC CTTCAGACAG CGAGGCTAGG ATGGCCTCTT TTCCCCAACC TTGGTTTGTG 
GTTATAAAGG AACATCAAAA TCTCAGGAAT GGAAGTCAAC CTATGGCCCT CAAGGATGGT 
GAGTCAGCAG ATGTTAGCCG GTTTTCCTCT CCAGAGCCAG GGTCAGTAAA ACGGACCTCA 
TGGAGAAATG AACACCAGTT ATATAAGGAC ACAGAGCAAG GCTGCTGCAC TCCACCATCC 
AGTAATAGAG GCACCGGCTC TCAGTCAATG GAGCAGAGCT TTTCTGTCCC CTCCTATGAG 
GCACAGCCCA TTGCTTTGGG GGTTGAGAAG CCACAGTCTC TCCTATCAGC TAACCCTTAT 
TGCAACAAAG GGCCCCAGAT CCACCACACC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

FGF2 and EGF Are Required for Self-Renewal and Organoid Formation of Canine Normal and Tumor Breast Stem Cells.
Journal of cellular biochemistry118(3)570-584(2017 03)
Cocola C,Molgora S,Piscitelli E,Veronesi MC,Greco M,Bragato C,Moro M,Crosti M,Gray B,Milanesi L,Grieco V,Luvoni GC,Kehler J,Bellipanni G,Reinbold R,Zucchi I,Giordano A

Expression and localization of epidermal growth factor, transforming growth factor-α and epidermal growth factor receptor in the canine testis.
The Journal of reproduction and development62(1)59-64(2016)
Tamada H,Takemoto K,Tominaga M,Kawate N,Takahashi M,Hatoya S,Matsuyama S,Inaba T,Sawada T

Matrix stiffening sensitizes epithelial cells to EGF and enables the loss of contact inhibition of proliferation.
Journal of cell science124(Pt 8)1280-7(2011 Apr)
Kim JH,Asthagiri AR

Positive correlation of steroid hormones and EGF in canine mammary cancer.
The Journal of steroid biochemistry and molecular biology115(1-2)9-13(2009 May)
Queiroga FL,Pérez-Alenza D,Silvan G,Peña L,Illera JC

Epithelial growth factor-induced phosphorylation of caveolin 1 at tyrosine 14 stimulates caveolae formation in epithelial cells.
The Journal of biological chemistry281(8)4570-9(2006 Feb)
Orlichenko L,Huang B,Krueger E,McNiven MA

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EGF cDNA ORF clone, Canis lupus familiaris(Dog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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