BOLA-DRB3 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	BOLA-DRB3
Entrez Gene ID	282530
Full Name	major histocompatibility complex, class II, DRB3
Synonyms	BLA-DRB3,Bota-DRB01,Bota-DRB02,Bota-DRB04,Bota-DRB07,Bota-DRB21,Bota-DRB22,LA-DRB
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Genome	Chromosome: 23 Map Location: 23q21

mRNA and Protein(s)

mRNA	Protein	Name
NM_001012680.2	NP_001012698.2	major histocompatibility complex, class II, DRB3 precursor
XM_024984096.1	XP_024839864.1	major histocompatibility complex, class II, DRB3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Bovine leukemia virus proviral load is more strongly associated with bovine major histocompatibility complex class II DRB3 polymorphism than with DQA1 polymorphism in Holstein cow in Japan.
Takeshima SN, Ohno A, Aida Y
Retrovirology16(1)14(2019 05)

Co-infection with Mycobacterium bovis does not alter the response to bovine leukemia virus in BoLA DRB3*0902, genetically resistant cattle.
Lützelschwab CM, Forletti A, Cepeda R, Esteban EN, Confalonieri O, Gutiérrez SE
Research in veterinary science10910-16(2016 Dec)

Association of BoLA-DRB3.2 Alleles with BLV Infection Profiles (Persistent Lymphocytosis/Lymphosarcoma) and Lymphocyte Subsets in ian Holstein Cattle.
Nikbakht Brujeni G, Ghorbanpour R, Esmailnejad A
Biochemical genetics54(2)194-207(2016 Apr)

Locus BoLA-DRB3 is just an ordinary site of the polygene when explaining genetic variance of somatic cell count and milk yield.
Oprzadek J, Sender G, Pawlik A, Lukaszewicz M
The Journal of dairy research82(4)449-52(2015 Nov)

Assessment of biodiversity in Chilean cattle using the distribution of major histocompatibility complex class II BoLA-DRB3 allele.
Takeshima SN, Miyasaka T, Matsumoto Y, Xue G, Diaz Vde L, Rogberg-Muñoz A, Giovambattista G, Ortiz M, Oltra J, Kanemaki M, Onuma M, Aida Y
Tissue antigens85(1)35-44(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BOLA-DRB3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BOLA-DRB3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa01393	NM_001012680.2 Latest version!	Bos taurus major histocompatibility complex, class II, DRB3 (BOLA-DRB3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OBa229809	XM_024984096.1 Latest version!	Bos taurus major histocompatibility complex, class II, DRB3 (BOLA-DRB3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BOLA-DRB3 ( NM_001012680.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001012698.2 Bos taurus major histocompatibility complex, class II, DRB3 (BOLA-DRB3), mRNA.

Price & Availability↑

CloneID	OBa01393
Clone ID Related Accession (Same CDS sequence)	NM_001012680.2
Accession Version	NM_001012680.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1563724800000
Organism	Bos taurus(cattle)
Product	major histocompatibility complex, class II, DRB3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U77067.1. On or before Jan 31, 2016 this sequence version replaced XM_015475270.1, XM_015468907.1, NM_001015523.1, XM_581666.1, NM_001012680.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U77067.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGGTGTGCC TGTATTTCTC TGGAGGCTCC TGGATGGCAG CTCTGATAGT GATGCTGATG 
GTGCTGTGCC CTCCCCTGGC CTGGGCCAGG GAGATCCAAC CACATTTCCT GGAGTATTAT 
AAGGGCGAGT GTCATTTCTT CAACGGGACC GAGCGGGTGC GGTTCCTGGA CAGACACTTC 
TATAATGGAG AAGAGTTCGT GCGCTTCGAC AGCGACTGGG GCGAGTTCCG GGCGGTGACC 
GAGCTGGGGC GGCCGGCCGC CGAGCACTGG AACAGCCAGA AGGACTTCCT GGAGCAGAAG 
CGGGCCGAGG TGGACAGGGT GTGCAGACAC AACTACGGGG GTGTGGAGAG TTTCACTGTG 
CAGCGGCGAG TGGAGCCTAC AGTGACTGTG TATCCTGCAA AGACTCAGCC CCTGCAGCAC 
CACAACCTCC TGGTCTGCTC TGTGAACGGT TTCTACCCAG GCCACATTGA AGTCAGGTGG 
TTCCGGAACG GCCATGAAGA GGAGGCTGGG GTGATCTCCA CAGGCCTGAT CCAGAATGGA 
GACTGGACCT TCCAGACCAT GGTGATGCTT GAAACAGTTC CTCAGAGTGG AGAGGTCTAC 
ACCTGCCAAG TGGATCACCC CAAGCGGACG AGCCCTATCA CAGTAGAATG GAGGGCACGG 
TCTGACTCTG CTCAGAGCAA GATGATGAGT GGAGTCGGGG GCTTCGTTCT GGGTCTGCTC 
TTCCTTGCCG TGGGGCTCTT CATCTACTTC AGGAATCAGA AAGGACGCCC TACACTTCAG 
CCAACAGGGC TCCTGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012698.2
CDS	52..852
Translation	MVCLYFSGGSWMAALIVMLMVLCPPLAWAREIQPHFLEYYKGECHFFNGTERVRFLDRHFYNGEEFVRFDSDWGEFRAVTELGRPAAEHWNSQKDFLEQKRAEVDRVCRHNYGGVESFTVQRRVEPTVTVYPAKTQPLQHHNLLVCSVNGFYPGHIEVRWFRNGHEEEAGVISTGLIQNGDWTFQTMVMLETVPQSGEVYTCQVDHPKRTSPITVEWRARSDSAQSKMMSGVGGFVLGLLFLAVGLFIYFRNQKGRPTLQPTGLLS

Target ORF information:

RefSeq Version	NM_001012680.2
Organism	Bos taurus(cattle)
Definition	Bos taurus major histocompatibility complex, class II, DRB3 (BOLA-DRB3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012680.2

ORF Insert Sequence:

ATGGTGTGCC TGTATTTCTC TGGAGGCTCC TGGATGGCAG CTCTGATAGT GATGCTGATG 
GTGCTGTGCC CTCCCCTGGC CTGGGCCAGG GAGATCCAAC CACATTTCCT GGAGTATTAT 
AAGGGCGAGT GTCATTTCTT CAACGGGACC GAGCGGGTGC GGTTCCTGGA CAGACACTTC 
TATAATGGAG AAGAGTTCGT GCGCTTCGAC AGCGACTGGG GCGAGTTCCG GGCGGTGACC 
GAGCTGGGGC GGCCGGCCGC CGAGCACTGG AACAGCCAGA AGGACTTCCT GGAGCAGAAG 
CGGGCCGAGG TGGACAGGGT GTGCAGACAC AACTACGGGG GTGTGGAGAG TTTCACTGTG 
CAGCGGCGAG TGGAGCCTAC AGTGACTGTG TATCCTGCAA AGACTCAGCC CCTGCAGCAC 
CACAACCTCC TGGTCTGCTC TGTGAACGGT TTCTACCCAG GCCACATTGA AGTCAGGTGG 
TTCCGGAACG GCCATGAAGA GGAGGCTGGG GTGATCTCCA CAGGCCTGAT CCAGAATGGA 
GACTGGACCT TCCAGACCAT GGTGATGCTT GAAACAGTTC CTCAGAGTGG AGAGGTCTAC 
ACCTGCCAAG TGGATCACCC CAAGCGGACG AGCCCTATCA CAGTAGAATG GAGGGCACGG 
TCTGACTCTG CTCAGAGCAA GATGATGAGT GGAGTCGGGG GCTTCGTTCT GGGTCTGCTC 
TTCCTTGCCG TGGGGCTCTT CATCTACTTC AGGAATCAGA AAGGACGCCC TACACTTCAG 
CCAACAGGGC TCCTGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BOLA-DRB3 ( XM_024984096.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839864.1 Bos taurus major histocompatibility complex, class II, DRB3 (BOLA-DRB3), mRNA.

Price & Availability↑

CloneID	OBa229809
Clone ID Related Accession (Same CDS sequence)	XM_024984096.1
Accession Version	XM_024984096.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	major histocompatibility complex, class II, DRB3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTGCC TGTATTTCTC TGGAGGCTCC TGGATGGCAG CTCTGATAGT GATGCTGATG 
GTGCTGTGCC CTCCCCTGGC CTGGGCCAGG GAGATCCAAC CACATTTCCT GGAGTATTAT 
AAGAGAGAGT GTCATTTCTT CAACGGGACC GAGCGGGTGC GGTTCCTGGA CAGATACTTC 
CATAATGGAG AAGAGTACGT GCGCTTCGAC AGCGACTGGG GCGAGTACCG GGCGGTGACC 
GAGCTGGGGC AGCGGGTCGC CGAGTACTGC AACAGCCAGA AGGACTTCCT GGAGCGGGCG 
CGGGCCGCGG TGGACACGTA CTGCAGACAC AACTACGGGG TCGGTGAGAG TTTCACTGTG 
CAGCGGCGAG TGGAGCCTAT AGTGACTGTG TATCCTGCAA AGACCCAGCC CCTGCAGCAC 
CACAACCTCC TGGTCTGCTC TGTGAACGGT TTCTACCCAG GCCACATTGA AGTCAGGTGG 
TTCCGGAATG GCCATGAAGA GGAGGCTGGG GTGATCTCCA CAGGCCTGAT CCAGAATGGA 
GACTGGACCT TCCAGACCAT GGTGATGCTT GAAACAGTTC CTCAGAGTGG AGAGGTCTAC 
ACCTGCCAAG TGGAGCACCC CAGCCGGACA AGCCCTATCA CAGTGGAATG GAGGGCACGG 
TCTGACTCTG CTCAGAGCAA GATGATGAGT GGAGTCGGGG GCTTCGTTCT GGGTCTGCTC 
TTCCTTGCGG TGGGGCTCTT CATCTACTTC AGGAATCAGA AAGGACACCC TACACTTCAG 
CCAACAGGGC TCCTGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839864.1
CDS	89..889
Translation	MVCLYFSGGSWMAALIVMLMVLCPPLAWAREIQPHFLEYYKRECHFFNGTERVRFLDRYFHNGEEYVRFDSDWGEYRAVTELGQRVAEYCNSQKDFLERARAAVDTYCRHNYGVGESFTVQRRVEPIVTVYPAKTQPLQHHNLLVCSVNGFYPGHIEVRWFRNGHEEEAGVISTGLIQNGDWTFQTMVMLETVPQSGEVYTCQVEHPSRTSPITVEWRARSDSAQSKMMSGVGGFVLGLLFLAVGLFIYFRNQKGHPTLQPTGLLS

Target ORF information:

RefSeq Version	XM_024984096.1
Organism	Bos taurus(cattle)
Definition	Bos taurus major histocompatibility complex, class II, DRB3 (BOLA-DRB3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984096.1

ORF Insert Sequence:

ATGGTGTGCC TGTATTTCTC TGGAGGCTCC TGGATGGCAG CTCTGATAGT GATGCTGATG 
GTGCTGTGCC CTCCCCTGGC CTGGGCCAGG GAGATCCAAC CACATTTCCT GGAGTATTAT 
AAGAGAGAGT GTCATTTCTT CAACGGGACC GAGCGGGTGC GGTTCCTGGA CAGATACTTC 
CATAATGGAG AAGAGTACGT GCGCTTCGAC AGCGACTGGG GCGAGTACCG GGCGGTGACC 
GAGCTGGGGC AGCGGGTCGC CGAGTACTGC AACAGCCAGA AGGACTTCCT GGAGCGGGCG 
CGGGCCGCGG TGGACACGTA CTGCAGACAC AACTACGGGG TCGGTGAGAG TTTCACTGTG 
CAGCGGCGAG TGGAGCCTAT AGTGACTGTG TATCCTGCAA AGACCCAGCC CCTGCAGCAC 
CACAACCTCC TGGTCTGCTC TGTGAACGGT TTCTACCCAG GCCACATTGA AGTCAGGTGG 
TTCCGGAATG GCCATGAAGA GGAGGCTGGG GTGATCTCCA CAGGCCTGAT CCAGAATGGA 
GACTGGACCT TCCAGACCAT GGTGATGCTT GAAACAGTTC CTCAGAGTGG AGAGGTCTAC 
ACCTGCCAAG TGGAGCACCC CAGCCGGACA AGCCCTATCA CAGTGGAATG GAGGGCACGG 
TCTGACTCTG CTCAGAGCAA GATGATGAGT GGAGTCGGGG GCTTCGTTCT GGGTCTGCTC 
TTCCTTGCGG TGGGGCTCTT CATCTACTTC AGGAATCAGA AAGGACACCC TACACTTCAG 
CCAACAGGGC TCCTGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Bovine leukemia virus proviral load is more strongly associated with bovine major histocompatibility complex class II DRB3 polymorphism than with DQA1 polymorphism in Holstein cow in Japan.
Retrovirology16(1)14(2019 05)
Takeshima SN,Ohno A,Aida Y

Co-infection with Mycobacterium bovis does not alter the response to bovine leukemia virus in BoLA DRB3*0902, genetically resistant cattle.
Research in veterinary science10910-16(2016 Dec)
Lützelschwab CM,Forletti A,Cepeda R,Esteban EN,Confalonieri O,Gutiérrez SE

Association of BoLA-DRB3.2 Alleles with BLV Infection Profiles (Persistent Lymphocytosis/Lymphosarcoma) and Lymphocyte Subsets in Iranian Holstein Cattle.
Biochemical genetics54(2)194-207(2016 Apr)
Nikbakht Brujeni G,Ghorbanpour R,Esmailnejad A

Locus BoLA-DRB3 is just an ordinary site of the polygene when explaining genetic variance of somatic cell count and milk yield.
The Journal of dairy research82(4)449-52(2015 Nov)
Oprzadek J,Sender G,Pawlik A,Lukaszewicz M

Assessment of biodiversity in Chilean cattle using the distribution of major histocompatibility complex class II BoLA-DRB3 allele.
Tissue antigens85(1)35-44(2015 Jan)
Takeshima SN,Miyasaka T,Matsumoto Y,Xue G,Diaz Vde L,Rogberg-Muñoz A,Giovambattista G,Ortiz M,Oltra J,Kanemaki M,Onuma M,Aida Y