LOC102654520 cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC102654520
Entrez Gene ID	102654520
Full Name	putative uncharacterized protein DDB_G0282133
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016917117.2	XP_016772606.2	putative uncharacterized protein DDB_G0282133
XM_016917117.1	XP_016772606.1	putative uncharacterized protein DDB_G0282133

Pathways from BioSystems
Gene Ontology

The following LOC102654520 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC102654520 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHd16569	XM_016917117.1 Latest version!	Apis mellifera putative uncharacterized protein DDB_G0282133 (LOC102654520), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHd28073	XM_016917117.2 Latest version!	Apis mellifera putative uncharacterized protein DDB_G0282133 (LOC102654520), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC102654520 ( XM_016917117.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_016772606.1 Apis mellifera putative uncharacterized protein DDB_G0282133 (LOC102654520), mRNA.

Price & Availability↑

CloneID	OHd16569
Clone ID Related Accession (Same CDS sequence)	XM_016917117.1
Accession Version	XM_016917117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1463932800000
Organism	Apis mellifera(honey bee)
Product	putative uncharacterized protein DDB_G0282133
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007085.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis mellifera Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGGTA TCATTACCAT TTTGCTCATC TTCTCCATTG ACGCTTATAC GCATGGCGCT 
CCTTTCGATG AATCGGCGGA ATGGGTGTCC GCGATTGGAA AACTCGGAAG AGAACAAAAT 
TCGAAGGAGA GGAATCCATT CGTTTGGATG CAGAATTCTG AGCGGAAAAG AAATGGATCG 
AACGTGGAAT ATCTACAATG GTTCGTGAAA AATTGGAAAG ATAACGGCGA GAAGAATAGA 
AAGAAAGATA GGGAGAAAGT TTCTTTAAAC TTATCACGGT TAAAAAACAA GTCTGATTCT 
TTCGATATCA GTTCCGATTC CGAACAAACA AAATTGAGAA TTTTATCTGG TCAGAAGAAA 
TGGAATTCCA TGGGGAAAAA TTCGAATGAA GAGCAAGAAG AGAAAGAAGA AGAGGAAGAA 
TCTATAGAGA AAATGTCTGA CGAGTTAAGT ATTCGTAAAA AAAGATATTC CGACGAAAAA 
GATTCTCGTG AAAGAAATTC TGATAAAAAT GATCATCGTG AAAATAATAA TTTTTCCGAA 
TCTTGGAATA TGACGGATGA AACTGATTCG AGTTCGTTTG AAAGATCAAA TAAATATTTT 
GATTCGAGAA AAAAAAAAAG GAAACGCGAT TCAGAGGAAT ACGATAGTTG CAAAAGTTCG 
AGTTCTGGTT CCAGTTCGTG GGAGTTTGAT AGCGATGAGG AAAAATTCAA TGATTATTTA 
AAAGATTTGT TAAAGGAAAA CCAACATTAT GAGGAAATAA CCGAAATGTT GAAAAATTAC 
AAAACAAATG TTTATTACGA GAGATTGTAC GAATGGAATA AAGTCAAGTA TCTGGAATGG 
AGGAAAAATA TAAAATTGAA GAATAAAATA TTGGACGAAA AGGAAAATTT AAGTATGGAG 
ATATTAAGGA AAATTTGTAA TGTATCAACC AACATTAAAC GTAAAGAACA AGAAAATCGA 
TTAAAAGACT GCGTAGGATC TTACATATCT ACGAAGGATA AATTTAAATT TACAACGGAT 
TTATCATTGG TCCATTTTCA AGATACGAAT ATTAAAACAG ATGGTGAAAA ATCGCATCCT 
AACAGAAGTT CCGATAATGG ACATATACAT ACCGAAGGAA TGAAAAATTC TTCGACAAAC 
GAAGAAAAAT CTTTCGATAA TGGAAACAGA GGTGCAACTA TTGACGATGA ACAAAAAGAA 
AAAAAAGAAC AACAACAAGA AACAAAGTCT AGTTCGCGAA AAGATGAGAT AGATAATGAT 
GAGAAGCCAA CTGATCGAAA TCTTGATAAT TTAACACAAT TAATATGGTC TTCACGGATT 
GGGACAGAAG AAAGAACAGT AACAACGAAA GATGAAAACT TGACAAATTT TGAGAACGAT 
ACGAATAGAT CGGAAGAATC GAATGTAATT TGGAGTACAA TTCCTCATAA ATATTTACAA 
ACTGAATCTT CTGAAGCATC GAAAAATCGA TCGACGATTT TCGAAGAATC GAATTCGAAT 
GTTAGAGATA ATGATCGAAA CAACGATTTG AATAAGAATA CAATACCGAG CAATAATGGA 
AATGAATATA TACGACAATT CGACAATACC GAATCAAATG GATCAGAGGA TTTAAATATT 
AATGAATCCA CAGTGATTTC AGCAAATCAA CAAACAATTT CCCGATCAAC TTCACGAATC 
GAAGCTTCTA CGATATGGGC GAAAGATGGA TTAACTACAA TATCGAGTAT CAAATTCGAA 
ACAATTGATA ATAACGATAA TTTTACTACA TCTAGCGACA TTAACTTTAC AATTGCAACA 
AGCAACGATA TAAATAATAA TGAATCGTTC AATAGCTATA ATAACGTACA GCCAAATACT 
AGTTCCGATA AGTCTAACGA ATTAAGAAAT AAAGAAACGA TAGAAGCATC GAGTAGGATA 
CCAGATACAT CATCGTTATC GTCTGTATAT CAAATAGAAA ATTCTCAAGT AGCAACTTCA 
ATGATATCTA CAGATACGAA TAACGAGCAA ACAGCAACTT CTACTTCGCA AACCATAAAT 
TCTAGCGATT TTAGAGATAA AGATCAGATA ATGGAAACGT GGAATCAAAA ATCGGGAACA 
AATTATGTCG ATAATGTTTC GATTACAGCG AATATTAATA TTGAAATCAA TACTGTTCGG 
ACTGAAAATG AAACGAATAC TCTTGATTCG AGTGTAGCAA ATACTCAAAC AGCTTCAAAT 
TCGACGGTAG AAAAAGTACT ATCGATTGAA ACCTTAAGTC CCGAATCAGA AATAATATTT 
ATCGATATAT CGAGTAATAA TGAAAAAGCT GATACGGATA AAGTAAGCGG TATTTTGGAA 
AATAAATCAA CTACCGATGC TAATGGATGG ACAAATCCAG GTAACAGCGA ATCATCGACT 
GCAAGAGAAA ACGAAGAAAC GACGATAAAT TATCAAATTG ATGATTCGAT TGTGACAAGA 
AAAAAAGAAG AAGCTGAAGT TCAGTCAAAC GATTGGCAAA CGATTTTTCG TGGCAATAAC 
AGCAACGAAC AGAATGTTAG CGTAATTACA ATCGAATCGA ATAAAACTTT GATACAAGAT 
GATAATTTAA TTATTACCGA TATTAATGAA CAAACAGATT CAATTGTTGA AACGATAAGT 
GATAATGAAT CTACTACGAA AGATGCACAA ATAATATTAT CTTTCTCCCA AACTGAAAAG 
TTTGATATAT TCAAAACGGA ACGATCGATC GATGATCAAT CTAAAACTAC CGATTTTCTC 
AACGTCACGA ATAATGGAAA CAATGAAACT GATGAGAATT CTACCAGTAT TGACGAATCA 
ACGATCGAAA AAGAAGAAAT AACGACACTT TCTTCGGAAA ATTTTATTGC TACCGAAATA 
ACGATGACTC AAACAATATC TTCGCAATTA CCAGAAATAA TTATCGGAAC TGGTGACGAT 
ATATTTGAAA ATAGAACTGA AAATGAGAAT ATTGATAAAA TTACAACTTT GGAAAATTTA 
CAAACAACAT CGTTTTCTAT GCAAACTGAA AAATCTAATA CAGGTGAAAC GAAAGGAAGA 
TTATCTATCC AAACGGGAAC AGATTCAACC GCTTTCACAA ATGAAATAGA GGAAATAGAA 
GCATTTTCTT CGAATAGTAA AAATTTTAGC GTTACGGAAG CAGAATTGAC AACTGTAAAA 
TTATCTTCAA AGTTAGAAAA TGAATTCGTA ACTTTAGAAA ATATAGAAAC AACATCGTCT 
TTTCTTCAGA CGCTTTCGAA TAGATCTGAA GCAAACGAAC CGATCGAAGC ATCGAGTACT 
CGGACTGAAA TGATTCTTGT TGATAACAGC ACTATTGTAT CGATTAATGT AAACGATAGA 
ACAAACAAAG CGAATAATAA TTTGAAAGAT AATGAAAAAT CAACTAGTAG TGTTGATAAA 
ACAGATTCAA ATAAATTTAC AACTTTGAAA GATATCGAAT CTGATTATTC GTCCAATGAA 
TATGATAATA CTACAGAAGC GAAAGCTCAA ACTGTAAAAC TAGAAATAAT CGTTTCTGAT 
ATCAATGGCA CTAAAATGGA TAATTTCATA ATCATTGATA GTAGCAATAT TTTGGATCTT 
TTCAATGTAC CAACTGGCAA CGTGAAAATA AATTCCAATG TTAAAGAGCC AAATACACCA 
ATAACGGATA CTCAAATATA TAATACAGTG ATTTCAAAAG CAGTTACATC AGCTGAATTA 
ATTATAAATT CAAATCCCGA ACCTCTTGTA AATGAAATGC CTATTAAGCG AGGAAAATTA 
TCGGTAAAGA AAGTCGATAT TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016772606.1
CDS	78..3884
Translation	MKGIITILLIFSIDAYTHGAPFDESAEWVSAIGKLGREQNSKERNPFVWMQNSERKRNGSNVEYLQWFVKNWKDNGEKNRKKDREKVSLNLSRLKNKSDSFDISSDSEQTKLRILSGQKKWNSMGKNSNEEQEEKEEEEESIEKMSDELSIRKKRYSDEKDSRERNSDKNDHRENNNFSESWNMTDETDSSSFERSNKYFDSRKKKRKRDSEEYDSCKSSSSGSSSWEFDSDEEKFNDYLKDLLKENQHYEEITEMLKNYKTNVYYERLYEWNKVKYLEWRKNIKLKNKILDEKENLSMEILRKICNVSTNIKRKEQENRLKDCVGSYISTKDKFKFTTDLSLVHFQDTNIKTDGEKSHPNRSSDNGHIHTEGMKNSSTNEEKSFDNGNRGATIDDEQKEKKEQQQETKSSSRKDEIDNDEKPTDRNLDNLTQLIWSSRIGTEERTVTTKDENLTNFENDTNRSEESNVIWSTIPHKYLQTESSEASKNRSTIFEESNSNVRDNDRNNDLNKNTIPSNNGNEYIRQFDNTESNGSEDLNINESTVISANQQTISRSTSRIEASTIWAKDGLTTISSIKFETIDNNDNFTTSSDINFTIATSNDINNNESFNSYNNVQPNTSSDKSNELRNKETIEASSRIPDTSSLSSVYQIENSQVATSMISTDTNNEQTATSTSQTINSSDFRDKDQIMETWNQKSGTNYVDNVSITANINIEINTVRTENETNTLDSSVANTQTASNSTVEKVLSIETLSPESEIIFIDISSNNEKADTDKVSGILENKSTTDANGWTNPGNSESSTARENEETTINYQIDDSIVTRKKEEAEVQSNDWQTIFRGNNSNEQNVSVITIESNKTLIQDDNLIITDINEQTDSIVETISDNESTTKDAQIILSFSQTEKFDIFKTERSIDDQSKTTDFLNVTNNGNNETDENSTSIDESTIEKEEITTLSSENFIATEITMTQTISSQLPEIIIGTGDDIFENRTENENIDKITTLENLQTTSFSMQTEKSNTGETKGRLSIQTGTDSTAFTNEIEEIEAFSSNSKNFSVTEAELTTVKLSSKLENEFVTLENIETTSSFLQTLSNRSEANEPIEASSTRTEMILVDNSTIVSINVNDRTNKANNNLKDNEKSTSSVDKTDSNKFTTLKDIESDYSSNEYDNTTEAKAQTVKLEIIVSDINGTKMDNFIIIDSSNILDLFNVPTGNVKINSNVKEPNTPITDTQIYNTVISKAVTSAELIINSNPEPLVNEMPIKRGKLSVKKVDIN

Target ORF information:

RefSeq Version	XM_016917117.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera putative uncharacterized protein DDB_G0282133 (LOC102654520), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016917117.1

ORF Insert Sequence:

ATGAAAGGTA TCATTACCAT TTTGCTCATC TTCTCCATTG ACGCTTATAC GCATGGCGCT 
CCTTTCGATG AATCGGCGGA ATGGGTGTCC GCGATTGGAA AACTCGGAAG AGAACAAAAT 
TCGAAGGAGA GGAATCCATT CGTTTGGATG CAGAATTCTG AGCGGAAAAG AAATGGATCG 
AACGTGGAAT ATCTACAATG GTTCGTGAAA AATTGGAAAG ATAACGGCGA GAAGAATAGA 
AAGAAAGATA GGGAGAAAGT TTCTTTAAAC TTATCACGGT TAAAAAACAA GTCTGATTCT 
TTCGATATCA GTTCCGATTC CGAACAAACA AAATTGAGAA TTTTATCTGG TCAGAAGAAA 
TGGAATTCCA TGGGGAAAAA TTCGAATGAA GAGCAAGAAG AGAAAGAAGA AGAGGAAGAA 
TCTATAGAGA AAATGTCTGA CGAGTTAAGT ATTCGTAAAA AAAGATATTC CGACGAAAAA 
GATTCTCGTG AAAGAAATTC TGATAAAAAT GATCATCGTG AAAATAATAA TTTTTCCGAA 
TCTTGGAATA TGACGGATGA AACTGATTCG AGTTCGTTTG AAAGATCAAA TAAATATTTT 
GATTCGAGAA AAAAAAAAAG GAAACGCGAT TCAGAGGAAT ACGATAGTTG CAAAAGTTCG 
AGTTCTGGTT CCAGTTCGTG GGAGTTTGAT AGCGATGAGG AAAAATTCAA TGATTATTTA 
AAAGATTTGT TAAAGGAAAA CCAACATTAT GAGGAAATAA CCGAAATGTT GAAAAATTAC 
AAAACAAATG TTTATTACGA GAGATTGTAC GAATGGAATA AAGTCAAGTA TCTGGAATGG 
AGGAAAAATA TAAAATTGAA GAATAAAATA TTGGACGAAA AGGAAAATTT AAGTATGGAG 
ATATTAAGGA AAATTTGTAA TGTATCAACC AACATTAAAC GTAAAGAACA AGAAAATCGA 
TTAAAAGACT GCGTAGGATC TTACATATCT ACGAAGGATA AATTTAAATT TACAACGGAT 
TTATCATTGG TCCATTTTCA AGATACGAAT ATTAAAACAG ATGGTGAAAA ATCGCATCCT 
AACAGAAGTT CCGATAATGG ACATATACAT ACCGAAGGAA TGAAAAATTC TTCGACAAAC 
GAAGAAAAAT CTTTCGATAA TGGAAACAGA GGTGCAACTA TTGACGATGA ACAAAAAGAA 
AAAAAAGAAC AACAACAAGA AACAAAGTCT AGTTCGCGAA AAGATGAGAT AGATAATGAT 
GAGAAGCCAA CTGATCGAAA TCTTGATAAT TTAACACAAT TAATATGGTC TTCACGGATT 
GGGACAGAAG AAAGAACAGT AACAACGAAA GATGAAAACT TGACAAATTT TGAGAACGAT 
ACGAATAGAT CGGAAGAATC GAATGTAATT TGGAGTACAA TTCCTCATAA ATATTTACAA 
ACTGAATCTT CTGAAGCATC GAAAAATCGA TCGACGATTT TCGAAGAATC GAATTCGAAT 
GTTAGAGATA ATGATCGAAA CAACGATTTG AATAAGAATA CAATACCGAG CAATAATGGA 
AATGAATATA TACGACAATT CGACAATACC GAATCAAATG GATCAGAGGA TTTAAATATT 
AATGAATCCA CAGTGATTTC AGCAAATCAA CAAACAATTT CCCGATCAAC TTCACGAATC 
GAAGCTTCTA CGATATGGGC GAAAGATGGA TTAACTACAA TATCGAGTAT CAAATTCGAA 
ACAATTGATA ATAACGATAA TTTTACTACA TCTAGCGACA TTAACTTTAC AATTGCAACA 
AGCAACGATA TAAATAATAA TGAATCGTTC AATAGCTATA ATAACGTACA GCCAAATACT 
AGTTCCGATA AGTCTAACGA ATTAAGAAAT AAAGAAACGA TAGAAGCATC GAGTAGGATA 
CCAGATACAT CATCGTTATC GTCTGTATAT CAAATAGAAA ATTCTCAAGT AGCAACTTCA 
ATGATATCTA CAGATACGAA TAACGAGCAA ACAGCAACTT CTACTTCGCA AACCATAAAT 
TCTAGCGATT TTAGAGATAA AGATCAGATA ATGGAAACGT GGAATCAAAA ATCGGGAACA 
AATTATGTCG ATAATGTTTC GATTACAGCG AATATTAATA TTGAAATCAA TACTGTTCGG 
ACTGAAAATG AAACGAATAC TCTTGATTCG AGTGTAGCAA ATACTCAAAC AGCTTCAAAT 
TCGACGGTAG AAAAAGTACT ATCGATTGAA ACCTTAAGTC CCGAATCAGA AATAATATTT 
ATCGATATAT CGAGTAATAA TGAAAAAGCT GATACGGATA AAGTAAGCGG TATTTTGGAA 
AATAAATCAA CTACCGATGC TAATGGATGG ACAAATCCAG GTAACAGCGA ATCATCGACT 
GCAAGAGAAA ACGAAGAAAC GACGATAAAT TATCAAATTG ATGATTCGAT TGTGACAAGA 
AAAAAAGAAG AAGCTGAAGT TCAGTCAAAC GATTGGCAAA CGATTTTTCG TGGCAATAAC 
AGCAACGAAC AGAATGTTAG CGTAATTACA ATCGAATCGA ATAAAACTTT GATACAAGAT 
GATAATTTAA TTATTACCGA TATTAATGAA CAAACAGATT CAATTGTTGA AACGATAAGT 
GATAATGAAT CTACTACGAA AGATGCACAA ATAATATTAT CTTTCTCCCA AACTGAAAAG 
TTTGATATAT TCAAAACGGA ACGATCGATC GATGATCAAT CTAAAACTAC CGATTTTCTC 
AACGTCACGA ATAATGGAAA CAATGAAACT GATGAGAATT CTACCAGTAT TGACGAATCA 
ACGATCGAAA AAGAAGAAAT AACGACACTT TCTTCGGAAA ATTTTATTGC TACCGAAATA 
ACGATGACTC AAACAATATC TTCGCAATTA CCAGAAATAA TTATCGGAAC TGGTGACGAT 
ATATTTGAAA ATAGAACTGA AAATGAGAAT ATTGATAAAA TTACAACTTT GGAAAATTTA 
CAAACAACAT CGTTTTCTAT GCAAACTGAA AAATCTAATA CAGGTGAAAC GAAAGGAAGA 
TTATCTATCC AAACGGGAAC AGATTCAACC GCTTTCACAA ATGAAATAGA GGAAATAGAA 
GCATTTTCTT CGAATAGTAA AAATTTTAGC GTTACGGAAG CAGAATTGAC AACTGTAAAA 
TTATCTTCAA AGTTAGAAAA TGAATTCGTA ACTTTAGAAA ATATAGAAAC AACATCGTCT 
TTTCTTCAGA CGCTTTCGAA TAGATCTGAA GCAAACGAAC CGATCGAAGC ATCGAGTACT 
CGGACTGAAA TGATTCTTGT TGATAACAGC ACTATTGTAT CGATTAATGT AAACGATAGA 
ACAAACAAAG CGAATAATAA TTTGAAAGAT AATGAAAAAT CAACTAGTAG TGTTGATAAA 
ACAGATTCAA ATAAATTTAC AACTTTGAAA GATATCGAAT CTGATTATTC GTCCAATGAA 
TATGATAATA CTACAGAAGC GAAAGCTCAA ACTGTAAAAC TAGAAATAAT CGTTTCTGAT 
ATCAATGGCA CTAAAATGGA TAATTTCATA ATCATTGATA GTAGCAATAT TTTGGATCTT 
TTCAATGTAC CAACTGGCAA CGTGAAAATA AATTCCAATG TTAAAGAGCC AAATACACCA 
ATAACGGATA CTCAAATATA TAATACAGTG ATTTCAAAAG CAGTTACATC AGCTGAATTA 
ATTATAAATT CAAATCCCGA ACCTCTTGTA AATGAAATGC CTATTAAGCG AGGAAAATTA 
TCGGTAAAGA AAGTCGATAT TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102654520 ( XM_016917117.2 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_016772606.2 Apis mellifera putative uncharacterized protein DDB_G0282133 (LOC102654520), mRNA.

Price & Availability↑

CloneID	OHd28073
Clone ID Related Accession (Same CDS sequence)	XM_016917117.2
Accession Version	XM_016917117.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1537200000000
Organism	Apis mellifera(honey bee)
Product	putative uncharacterized protein DDB_G0282133
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037653.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 19, 2018 this sequence version replaced XM_016917117.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGGTA TCATTACCAT TTTGCTCATC TTCTCCATTG ACGCTTATAC GCATGGCGCT 
CCTTTCGATG AATCGGCGGA ATGGGTGTCC GCGATTGGAA AACTCGGAAG AGAACAAAAT 
TCGAAGGAGA GGAATCCATT CGTTTGGATG CAGAATTCTG AGCGGAAAAG AAATGGATCG 
AACGTGGAAT ATCTACAATG GTTCGTGAAA AATTGGAAAG ATAACGGCGA GAAGAATAGA 
AAGAAAGATA GGGAGAAAGT TTCTTTAAAC TTATCACGGT TAAAAAACAA GTCTGATTCT 
TTCGATATCA GTTCCGATTC CGAACAAACA AAATTGAGAA TTTTATCTGG TCAGAAGAAA 
TGGAATTCCG TGGGGAAAAA TTCGAATGAA GAGCAAGAAG AGAAAGAAGA AGAGGAAGAA 
TCTATAGAGA AAATGTCTGA CGAGTTAAGT ATTCGTAAAA AAAGATATTC CGGCGAAAAA 
GATTCTCGTG AAAGAAATTC TGATAAAAAT GATCATCGTG AAAATAATAA TTTTTCCGAA 
TCTTGGAATA TGACGGATGA AACTGATTCG AGTTCGTTTG AAAGATCAAA TAAATATTTT 
GATTCGAGAA AAAAAAAAAG GAAACGCGAT TCAGAGGAAT ACGATAGTCG CAAAAGTTCG 
AGTTCTGGTT CCAGTTCGTG GGAGTTTGAT AGCGATGAGG AAAAATTCAA TGATTATTTA 
AAAGATTTGT TAAAGGAAAA CCAACATTAT GAGGAAATAA CCGAAATGTT GAAAAATTAC 
AAAACAAATG TTTATTACGA GAGATTGTAC GAATGGAATA AAGTCAAGTA TCTGGAATGG 
AGGAAAAATA TAAAATTGAA GAATAAAATA TTGGACGAAA AGGAAAATTT AAGTATGGAG 
ATATTAAGGA AAATTTGTAA TGTATCAACC AACATTAAAC GTAAAGAACA AGAAAATCGA 
TTAAAAGACT GCGTAGGATC TTACATATCT ACGAAGGATA AATTTAAATT TACAACGGAT 
TTATCATTGG TCCATTTTCA AGATACGAAT ATTAAAACAG ATGGTGAAAA ATCGCATCCT 
AACAGAAGTT CCGATAATGG ACATATACAT ATCGAAGGAA TGAAAAATTC TTCGACAAAC 
GAAGAAAAAT CTTTCGATAA TGGAAACAGA GGTGCAACTA TTGACGATGA ACAAAAAGAA 
AAAAAAGAAC AACAACAAGA AACAAAGTCT AGTTCGCGAA AAGATGAGAT AGATAATGAT 
GAGAAGCCAA CTGATCGAAA TCTTGATAAT TTAACACAAT TAATATGGTC TTCACGGATT 
GGGACAGAAG AAAGAACAGT AACAACGAAA GATGAAAACT TGACAAATTT TGAGAACGAT 
ACGAATAGAT CGGAAGAATC AAATGTAATT TGGAGTACAA TTCCTCATAA ATATTTACAA 
ACTGAATCTT CTGAAGCATC GAAAAATCGA TCGACGATTT TCGAAGAATC GAATTCGAAT 
GTTAGAGATA ATGATCGAAA CAACGATTTG AATAAGAATA CAATACCGAG CAATAATGGA 
AATGAATATA TACGACAATT CGACAATACC GAATCAAATG GATCAGAGGA TTTAAATATT 
AATGAATCCA CAGTGATTTC AGCAAATCAA CAAACAATTT CCCGATCAAC TTCACGAATC 
GAAGCTTCTA CGATATGGGC GAAAGATGGA TTAACTACAA TATCGAGTAT CAAATTCGAA 
ACAATTGATA ATAACGATAA TTTTACTACA TCTAGCGACA TTAACTTTAC AATTGCAACA 
AGCAACGATA TAAATAATAA TGAATCGTTC AATAGCTATA ATAACGTACA GCCAAATACT 
AGTTCCGATA AGTCTAACGA ATTAAGAAAT AAAGAAACGA TAGAAGCATC GAGTAGGATA 
CCAGATACAT CATCGTTATC GTCTGTATAT CAAATAGAAA ATTCTCAAGT AGCAACTTCA 
ATGATATCTA CAGATACGAA TAACGAGCAA ACAGCAACTT CTACTTCGCA AACCATAAAT 
TCTAGCGATT TTAGAGATAA AGATCAGATG ATGGAAACGT GGAATCAAAA ATCGGGAACA 
AATTATGTCG ATAATGTTTC GATTACAGCG AATATTAATA TTGAAATCAA TACTGTTCGG 
ACTGAAAATG AAACGAATAC TCTTGATTCG AGTGTAGCAA ATACTCAAAC AGCTTCAAAT 
TCGACGGTAG AAAAAGTACT ATCGATTGAA ACCTTAAGTC CCGAATCAGA AATAATATTT 
ATCGATATAT CGAGTAATAA TGAAAAAGCT GATACGGATA AAGTAAGCGG TATTTTGGAA 
AATAAATCAA CTACCGATGC TAATGGATGG ACAAATCCAG GTAACAGCGA ATCATCGACT 
GCAAGAGAAA ACGAAGAAAC GACGATAAAT TATCAAATTG ATGATTCGAT TGTGACAAGA 
AAAAAAGAAG AAGCTGAAGT GTCAAACGAT TGGCAAACGA TTTTTCGTGG CAATAACAGC 
AACGAACAGA ATGTTAGCGT AATTACAATC GAATCGAATA AAACTTTGAT ACAAGATGAT 
AATTTAATTA TTACCGATAT TAATGAACAA ACAGATTCAA TTGTTGAAAC GATAAGTGAT 
AATGAATCTA CTACGAAAGA TGCACAAATA ATATTATCTT TCTCCCAAAC TGAAAAGTTT 
GATATATTCA AAACGGAACG ATCGATCGAT GATCAATCTA AAACTACCGA TTTTCTCAAC 
GTCACGAATA ATGGAAACAA TGAAACTGAT GAGAATTCTA CCAGTATTGA CGAATCAACG 
ATCGAAAAAG AAGAAATAAC GACACTTTCT TCGGAAAATT TTATTGCTAC CGAAATAACG 
ATGACTCAAA CAATATCTTC GCAATTACCA GAAATAATTA TCGGAACTGG TGACGATATA 
TTTGAAAATA GAACTGAAAA TGAGAATATT GATAAAATTA CAACTTTGGA AAATTTACAA 
ACAACATCGT TTTCTATGCA AACTGAAAAA TCTAATACAG GTGAAACGAA AGGAAGATTA 
TCTATCCAAA CGGGAACAGA TTCAACCGCT TTCACAAATG AAATAGAGGA AATAGAAGCA 
TTTTCTTCGA ATAGTAAAAA TTTTAGCGTT ACGGAAGCAG AATTGACAAC TGTAAAATTA 
TCTTCAAAGT TAGAAAATGA ATTCGTAACT TTAGAAAATA TAGAAACAAC ATCGTCTTTT 
CTTCAGACGC TTTCGAATAG ATCTGAAGCA AACGAACCGA TCGAAGCATC GAGTACTCGG 
ACTGAAATGA TTCTTGTTGA TAACAGCACT ATTGTATCGA TTAATGTAAA CGATAGAACA 
AACAAAGCGA ATAATAATTT GAAAGATAAT GAAAAATCAA CTAGTAGTGT TGATAAAACA 
GATTCAAATA AATTTACAAC TTTGAAAGAT ATCGAATCTG ATTATTCGTC CAATGAATAT 
GATAATACTA CAGAAGCGAA AGCTCAAACT GTAAAACTAG AAATAATCGT TTCTGATATC 
AATGGCACTA AAATGGATAA TTTCATAATC ATTGATAGTA GCAATATTTT GGATCTTTTC 
AATGTACCAA CTGGCAACGT GAAAATAAAT TCCAATGTTA AAGAGCCAAA TACACCAATA 
ACGGATACTC AAATATATAA TACAGTGATT TCAAAAGCAG TTACATCAGC TGAATTAATT 
ATAAATTCAA ATCCCGAACC TCTTGTAAAT GAAATGCCTA TTAAGCGAGG AAAATTATCG 
GTAAAGAAAG TCGATATTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016772606.2
CDS	78..3881
Translation	MKGIITILLIFSIDAYTHGAPFDESAEWVSAIGKLGREQNSKERNPFVWMQNSERKRNGSNVEYLQWFVKNWKDNGEKNRKKDREKVSLNLSRLKNKSDSFDISSDSEQTKLRILSGQKKWNSVGKNSNEEQEEKEEEEESIEKMSDELSIRKKRYSGEKDSRERNSDKNDHRENNNFSESWNMTDETDSSSFERSNKYFDSRKKKRKRDSEEYDSRKSSSSGSSSWEFDSDEEKFNDYLKDLLKENQHYEEITEMLKNYKTNVYYERLYEWNKVKYLEWRKNIKLKNKILDEKENLSMEILRKICNVSTNIKRKEQENRLKDCVGSYISTKDKFKFTTDLSLVHFQDTNIKTDGEKSHPNRSSDNGHIHIEGMKNSSTNEEKSFDNGNRGATIDDEQKEKKEQQQETKSSSRKDEIDNDEKPTDRNLDNLTQLIWSSRIGTEERTVTTKDENLTNFENDTNRSEESNVIWSTIPHKYLQTESSEASKNRSTIFEESNSNVRDNDRNNDLNKNTIPSNNGNEYIRQFDNTESNGSEDLNINESTVISANQQTISRSTSRIEASTIWAKDGLTTISSIKFETIDNNDNFTTSSDINFTIATSNDINNNESFNSYNNVQPNTSSDKSNELRNKETIEASSRIPDTSSLSSVYQIENSQVATSMISTDTNNEQTATSTSQTINSSDFRDKDQMMETWNQKSGTNYVDNVSITANINIEINTVRTENETNTLDSSVANTQTASNSTVEKVLSIETLSPESEIIFIDISSNNEKADTDKVSGILENKSTTDANGWTNPGNSESSTARENEETTINYQIDDSIVTRKKEEAEVSNDWQTIFRGNNSNEQNVSVITIESNKTLIQDDNLIITDINEQTDSIVETISDNESTTKDAQIILSFSQTEKFDIFKTERSIDDQSKTTDFLNVTNNGNNETDENSTSIDESTIEKEEITTLSSENFIATEITMTQTISSQLPEIIIGTGDDIFENRTENENIDKITTLENLQTTSFSMQTEKSNTGETKGRLSIQTGTDSTAFTNEIEEIEAFSSNSKNFSVTEAELTTVKLSSKLENEFVTLENIETTSSFLQTLSNRSEANEPIEASSTRTEMILVDNSTIVSINVNDRTNKANNNLKDNEKSTSSVDKTDSNKFTTLKDIESDYSSNEYDNTTEAKAQTVKLEIIVSDINGTKMDNFIIIDSSNILDLFNVPTGNVKINSNVKEPNTPITDTQIYNTVISKAVTSAELIINSNPEPLVNEMPIKRGKLSVKKVDIN

Target ORF information:

RefSeq Version	XM_016917117.2
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera putative uncharacterized protein DDB_G0282133 (LOC102654520), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016917117.2

ORF Insert Sequence:

ATGAAAGGTA TCATTACCAT TTTGCTCATC TTCTCCATTG ACGCTTATAC GCATGGCGCT 
CCTTTCGATG AATCGGCGGA ATGGGTGTCC GCGATTGGAA AACTCGGAAG AGAACAAAAT 
TCGAAGGAGA GGAATCCATT CGTTTGGATG CAGAATTCTG AGCGGAAAAG AAATGGATCG 
AACGTGGAAT ATCTACAATG GTTCGTGAAA AATTGGAAAG ATAACGGCGA GAAGAATAGA 
AAGAAAGATA GGGAGAAAGT TTCTTTAAAC TTATCACGGT TAAAAAACAA GTCTGATTCT 
TTCGATATCA GTTCCGATTC CGAACAAACA AAATTGAGAA TTTTATCTGG TCAGAAGAAA 
TGGAATTCCG TGGGGAAAAA TTCGAATGAA GAGCAAGAAG AGAAAGAAGA AGAGGAAGAA 
TCTATAGAGA AAATGTCTGA CGAGTTAAGT ATTCGTAAAA AAAGATATTC CGGCGAAAAA 
GATTCTCGTG AAAGAAATTC TGATAAAAAT GATCATCGTG AAAATAATAA TTTTTCCGAA 
TCTTGGAATA TGACGGATGA AACTGATTCG AGTTCGTTTG AAAGATCAAA TAAATATTTT 
GATTCGAGAA AAAAAAAAAG GAAACGCGAT TCAGAGGAAT ACGATAGTCG CAAAAGTTCG 
AGTTCTGGTT CCAGTTCGTG GGAGTTTGAT AGCGATGAGG AAAAATTCAA TGATTATTTA 
AAAGATTTGT TAAAGGAAAA CCAACATTAT GAGGAAATAA CCGAAATGTT GAAAAATTAC 
AAAACAAATG TTTATTACGA GAGATTGTAC GAATGGAATA AAGTCAAGTA TCTGGAATGG 
AGGAAAAATA TAAAATTGAA GAATAAAATA TTGGACGAAA AGGAAAATTT AAGTATGGAG 
ATATTAAGGA AAATTTGTAA TGTATCAACC AACATTAAAC GTAAAGAACA AGAAAATCGA 
TTAAAAGACT GCGTAGGATC TTACATATCT ACGAAGGATA AATTTAAATT TACAACGGAT 
TTATCATTGG TCCATTTTCA AGATACGAAT ATTAAAACAG ATGGTGAAAA ATCGCATCCT 
AACAGAAGTT CCGATAATGG ACATATACAT ATCGAAGGAA TGAAAAATTC TTCGACAAAC 
GAAGAAAAAT CTTTCGATAA TGGAAACAGA GGTGCAACTA TTGACGATGA ACAAAAAGAA 
AAAAAAGAAC AACAACAAGA AACAAAGTCT AGTTCGCGAA AAGATGAGAT AGATAATGAT 
GAGAAGCCAA CTGATCGAAA TCTTGATAAT TTAACACAAT TAATATGGTC TTCACGGATT 
GGGACAGAAG AAAGAACAGT AACAACGAAA GATGAAAACT TGACAAATTT TGAGAACGAT 
ACGAATAGAT CGGAAGAATC AAATGTAATT TGGAGTACAA TTCCTCATAA ATATTTACAA 
ACTGAATCTT CTGAAGCATC GAAAAATCGA TCGACGATTT TCGAAGAATC GAATTCGAAT 
GTTAGAGATA ATGATCGAAA CAACGATTTG AATAAGAATA CAATACCGAG CAATAATGGA 
AATGAATATA TACGACAATT CGACAATACC GAATCAAATG GATCAGAGGA TTTAAATATT 
AATGAATCCA CAGTGATTTC AGCAAATCAA CAAACAATTT CCCGATCAAC TTCACGAATC 
GAAGCTTCTA CGATATGGGC GAAAGATGGA TTAACTACAA TATCGAGTAT CAAATTCGAA 
ACAATTGATA ATAACGATAA TTTTACTACA TCTAGCGACA TTAACTTTAC AATTGCAACA 
AGCAACGATA TAAATAATAA TGAATCGTTC AATAGCTATA ATAACGTACA GCCAAATACT 
AGTTCCGATA AGTCTAACGA ATTAAGAAAT AAAGAAACGA TAGAAGCATC GAGTAGGATA 
CCAGATACAT CATCGTTATC GTCTGTATAT CAAATAGAAA ATTCTCAAGT AGCAACTTCA 
ATGATATCTA CAGATACGAA TAACGAGCAA ACAGCAACTT CTACTTCGCA AACCATAAAT 
TCTAGCGATT TTAGAGATAA AGATCAGATG ATGGAAACGT GGAATCAAAA ATCGGGAACA 
AATTATGTCG ATAATGTTTC GATTACAGCG AATATTAATA TTGAAATCAA TACTGTTCGG 
ACTGAAAATG AAACGAATAC TCTTGATTCG AGTGTAGCAA ATACTCAAAC AGCTTCAAAT 
TCGACGGTAG AAAAAGTACT ATCGATTGAA ACCTTAAGTC CCGAATCAGA AATAATATTT 
ATCGATATAT CGAGTAATAA TGAAAAAGCT GATACGGATA AAGTAAGCGG TATTTTGGAA 
AATAAATCAA CTACCGATGC TAATGGATGG ACAAATCCAG GTAACAGCGA ATCATCGACT 
GCAAGAGAAA ACGAAGAAAC GACGATAAAT TATCAAATTG ATGATTCGAT TGTGACAAGA 
AAAAAAGAAG AAGCTGAAGT GTCAAACGAT TGGCAAACGA TTTTTCGTGG CAATAACAGC 
AACGAACAGA ATGTTAGCGT AATTACAATC GAATCGAATA AAACTTTGAT ACAAGATGAT 
AATTTAATTA TTACCGATAT TAATGAACAA ACAGATTCAA TTGTTGAAAC GATAAGTGAT 
AATGAATCTA CTACGAAAGA TGCACAAATA ATATTATCTT TCTCCCAAAC TGAAAAGTTT 
GATATATTCA AAACGGAACG ATCGATCGAT GATCAATCTA AAACTACCGA TTTTCTCAAC 
GTCACGAATA ATGGAAACAA TGAAACTGAT GAGAATTCTA CCAGTATTGA CGAATCAACG 
ATCGAAAAAG AAGAAATAAC GACACTTTCT TCGGAAAATT TTATTGCTAC CGAAATAACG 
ATGACTCAAA CAATATCTTC GCAATTACCA GAAATAATTA TCGGAACTGG TGACGATATA 
TTTGAAAATA GAACTGAAAA TGAGAATATT GATAAAATTA CAACTTTGGA AAATTTACAA 
ACAACATCGT TTTCTATGCA AACTGAAAAA TCTAATACAG GTGAAACGAA AGGAAGATTA 
TCTATCCAAA CGGGAACAGA TTCAACCGCT TTCACAAATG AAATAGAGGA AATAGAAGCA 
TTTTCTTCGA ATAGTAAAAA TTTTAGCGTT ACGGAAGCAG AATTGACAAC TGTAAAATTA 
TCTTCAAAGT TAGAAAATGA ATTCGTAACT TTAGAAAATA TAGAAACAAC ATCGTCTTTT 
CTTCAGACGC TTTCGAATAG ATCTGAAGCA AACGAACCGA TCGAAGCATC GAGTACTCGG 
ACTGAAATGA TTCTTGTTGA TAACAGCACT ATTGTATCGA TTAATGTAAA CGATAGAACA 
AACAAAGCGA ATAATAATTT GAAAGATAAT GAAAAATCAA CTAGTAGTGT TGATAAAACA 
GATTCAAATA AATTTACAAC TTTGAAAGAT ATCGAATCTG ATTATTCGTC CAATGAATAT 
GATAATACTA CAGAAGCGAA AGCTCAAACT GTAAAACTAG AAATAATCGT TTCTGATATC 
AATGGCACTA AAATGGATAA TTTCATAATC ATTGATAGTA GCAATATTTT GGATCTTTTC 
AATGTACCAA CTGGCAACGT GAAAATAAAT TCCAATGTTA AAGAGCCAAA TACACCAATA 
ACGGATACTC AAATATATAA TACAGTGATT TCAAAAGCAG TTACATCAGC TGAATTAATT 
ATAAATTCAA ATCCCGAACC TCTTGTAAAT GAAATGCCTA TTAAGCGAGG AAAATTATCG 
GTAAAGAAAG TCGATATTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC102654520 cDNA ORF clone, Apis mellifera(honey bee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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