LOC110509489 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC110509489
Entrez Gene ID	110509489
Full Name	spectrin alpha chain, non-erythrocytic 1-like
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021590383.1	XP_021446058.1	spectrin alpha chain, non-erythrocytic 1-like

Pathways from BioSystems
Gene Ontology

The following LOC110509489 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC110509489 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf37525	XM_021590383.1 Latest version!	Oncorhynchus mykiss spectrin alpha chain, non-erythrocytic 1-like (LOC110509489), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC110509489 ( XM_021590383.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021446058.1 Oncorhynchus mykiss spectrin alpha chain, non-erythrocytic 1-like (LOC110509489), mRNA.

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CloneID	OOf37525
Clone ID Related Accession (Same CDS sequence)	XM_021590383.1
Accession Version	XM_021590383.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	spectrin alpha chain, non-erythrocytic 1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035105.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACCA TTGAAATCCA AGACCTAGGG CAACAGCGGG AGACAAGTCG TCTGCCTAGG 
GAGACTTTGG CCACCACCTC TCCTCAGAGA ATGGATACCA GTGGGGTGAA AGTGCTGGAG 
TCTGCCGATG ACATCCAGGA GCGCCGGCAG CAGGTGTTGG ATCGCTACCG GCGCTTCAAG 
GAGCTGTCGG GTGTGCGCAG GCAGAAGCTG GAGGACTCCT ACCGTTTCCA GTTCTTCCGC 
CGCGATGCCG ATGAGCTGGA GAAGTGGATC CAGGAGAAGC TGCAGATCGC CTCCGATGAG 
AACTACAAGG ACCCCACCAA CCTCCAGGGC AAGCTCCAGA AACACCAGGC CTTCGAGGCG 
GAGGTGCAGG CCAACGCTGG AGCCATCATC AAGCTTGATG AGACTGGCAA CCTCATGACC 
TCAGAGGGTC ACTTTGCCTC TGAAACCATC CGCACTCGTC TGGAGGAGCT GCATCGTCTT 
TGGGACCTGC TGCTGCAGAA GACCAAGGAG AAAGGTGTTC GTCTGCTGCA GGCCCAGAAG 
CTGGTGCAGT ACCTGCGCGA GTGTGAGGAT GCCCTGGACT GGATTAGTGA CAAGGAGGCC 
ATGGCCACCT CGGAGGAGCT GGGCCAGGAC TTGGAGCATG TGGAGGTGCT CCAGAAGAAG 
TTTGAGGAGT TCCAGACGGA CCTGGCGGCT CACGAGGAGC GTGTGAACGA GGTGAACCAG 
CTGGCGGGCA AGCTGAGCCA GGAGTCCCAC CCCGAGGCGG AGCTCATCGT CCGCAAGCAG 
GAGGAGGTGA ACGCCGCCTG GCAGAGGCTC AAGGGCCTGG CACAGCAGAG GCAGGGCAGG 
CTGTTCGGAG CCGCCGAGGT GCAGCGCTTC AACAGGGATG TGGACGAGAC CATCAGCTGG 
ATCAAGGAGA AGGAGCAACT CATGGCGTCC GATGACTTTG GCCGGGACCT GGCCAGCGTT 
CAGGCCCTGC TGCGCAAGCA CGAGGGCCTG GAGAGAGACC TGGCTGCCCT GGAGGACAAG 
GTCAACACTC TGGGCGGAGA GGCTGAACGT CTGCAGCAGA CCCACCCCCA GAACGCTTCC 
CAGATCCACC TGAAGAGGGA CGAGCTCATC ACCAACTGGG AGCAGATCCG CACGCTGGCC 
GCCGAGCGCC ACGCCCACCT CAATGACTCC TACAGGCTGC AGCGCTACAC TGCAGACTTC 
CGTGACCTGA CCAGCTGGGT GACGGAGATG AAGGCCCTGA TCAACGCTGA CGAGCTGGCC 
AACGACGTGG CCGGAGCCGA GGCTCTTCTC GACCGCCACC AGGAGCACAA GGGTGAGATT 
GATGCCCATG AAGACAGCTT CAAAGCCACG GACGAAGCCG GTCAGGCCTT GCTCAACACC 
GGACACTACG CCTTAGAGGA GGTCAAGGAG AAGCTGGGTG TCCTGACTGA AGAGAAGGAG 
TCTCTGCTGG AGCTGTGGGA GGTGCGCAGG CAGCAGTACG AGCAGTGCAT GGACCTGCAG 
CTCTTCTACA GGGACACAGA GCAGGTTGAC AACTGGATGA GCAAGCAAGA GGCTTTCCTT 
CTGAACGAGG ACCTTGGTGA CTCTCTGGAC AGCGTGGAGG CCTTGCTTAA GAAACACGAG 
GACTTTGAGA AGTCCCTCAG CGCCCAGGAG GAGAAGATCA CTGCCCTGGA TGAATTTGCC 
ACCAAACTGA TCCAGAACAA CCACTATGCC AAGGAGGATG TTGCTACTCG TAGAGATGCT 
CTGCTGAGCC GCCGCAACGC CCTGCACGAG CGTGCTCAGT CCCGCCGCGC CGCTCTGGAG 
GACTCCTTCC ACCTGCAGCA GTTCTTCCGT GACTCGGACG AACTCAAGAG CTGGATCAAT 
GAGAAGATGA AGACGGCCAC CGACGAAGCC TACAAGGACC CGTCCAACCT GCAGGGCAAG 
GTCCAGAAGC ACCAGGCCTT CGAGGCGGAG CTGTCAGCCA ACCAGAGCCG CATCGATGCT 
CTGCAGAAGT CTGGCCAGGA GCTCCTAGAC GGGAAGCACT ACGCCGCCGA CGAGGTGTCT 
GTACGCATGG ACGAGGTCAG CTCCCAGTGG AAGAAGCTGC TGGAGGCCAC CGAACTCAAA 
GGCATCAAGC TGCGCGAGGC CAACCAGCAG CAGCAGTTCA ACAGAAACGT GGAGGACATT 
GAGCTGTGGC TGTATGAGGT TGAGGGCCAC CTGGCTTCAG ATGACTACGG CAAGGATCTG 
ACCAGCGTCC AGAACCTGCA GAAGAAGCAC GCCCTGCTGG AGGCTGACGT GGCTGCCCAC 
CAGGACCGCA TCGATGGCAT CACCATTCAG GCGCGTCAGT TCCAGGAAGC CGGTCACTTT 
GACGGCGACA ACATCAAGCG CAAGCAGGAG GCGCTGGTGG CGCGCTACGA TGCCCTCCGC 
GACCCCATGG CCGCCCGCAA GCAGAAGCTG TCAGACTCCC TGCGGCTGCA GCAGCTCTTC 
AGGGACGTGG AGGACGAGGA AACCTGGGTC CGGGAGAAGG AGCCCATCGC CGCCTCCACC 
AACCGGGGAA AAGACCTGAT CGGCGTCCAG AACTTGCTGA AGAAGCACCA GGCCCTGCAG 
GCGGAGATCG CTGGCCATGA GCCCCGCATC AAGGCCGTCA CCCAGAAAGG AGAGGCTATG 
GTGGAGGAAG GCCACTTTGC TGGGGAGGAG GTTAAGGTGA AGCTGGGGGA GCTGAACGGC 
CGCTGGGACA CCCTGAAAGG CAAGGCCGGG CAGCGCAGGC AGGACCTGGA GGACTCATTG 
CAGGCACAGC AGTACTTCGC CGACGCCAAC GAGGCAGAGT CCTGGATGAG GGAGAAAGAG 
CCCATCGTGG GCAGTCCCGA CTACGGCAAG GATGAGGATT CGGCTGAGGC CCTGCTGAAG 
AAGCACGAAG CCCTGATGTC TGACCTGAGT GCTTATGGCA CCAGCATCCA GGCTTTGAAG 
GAGCAGGCCC AATCCTGCAG GCAACAAGTT GCGCCCACCG ATGATGAGAC TGGCAAGGAG 
CTGGTGCTGG CCCTCTATGA CTACCAGGAG AAGAGCCCCC GTGAGGTCAC CATGAAGAAG 
GGAGACATCC TCACCCTGCT CAACAGTACC AACAAGGACT GGTGGAAGGT GGAGGTGAAC 
GACCGCCAGG GCTTTGTGCC AGCAGCCTAC GTCAAGAAGC TAGACCCCAC CCAGTCCTCC 
TCCAGGGAGA ACCTGCTGGA CGAGCAGGGC AGCATCGGCC TCCGCCAGGA CCAGATCGAG 
AACCAGCCGG GCGCAAAGGA GGCGTGCAGT GTGTCTGTAC GCATGAAGCA GGTGGAGGAA 
CTGTACGGCA CTCTCCTGGA GCTGGGCGAG AAACGCAAGG ACATGCTAGA AAAGAGCTGC 
AAGAAGTTCA TGCTGTTCCG CGAGGCTAAC GAACTGCAGC AGTGGATCAA CGAGAAGGAG 
GGAGCCCTCA CCAACGAGGA GGTGGGCTCT GACCTGGAGC AGGTGGAGGT GCTGCAGAAG 
AAGTTTGACG ACTTCCAGAA GGACCTGAAG GCCAACGAGT CTCGTCTGAG GGACATCAAC 
AAGGTGGCAT CTGAGCTGGA GTCTGAGGGC CTAATGGCAG AGGAGGCACA CATGGTCCAA 
GCTCAGGAAC AAGTAATGGT GGGTTCCGTT CCTGGCAAGG ATGATACCGA TTCCAAAAAT 
GCTTCACCAT GGAAGGAGCT GAACAACCGC TGGAGGTCCC TGCAGCAGCT GGCTGAGGAG 
AGGAGCAACA TGCTGGGGAG TGCCCATGAG GTGCAGAGGT TCCACAGGGA TGCGGATGAG 
ACCAAGGAGT GGATCGAGGA GAAGAACCAG GCCCTGAACA CCGACAACTA CGGCCACGAC 
CTGGCTAGCG TTCAGGCTCT GCAGCGCAAA CATGAGGGCT TTGAGAGAGA CCTGGCCGCC 
TTGGGAGACA AGGTGAAGTC TCTGGGGGAG ACGGCGGAGC GTCTGATCCA GTCCCACCCG 
GAGGCTGTGG ACGACATCCA GGAGAAGTGC ACAGAGCTCA ACACGGCCTG GAGCAGCCTA 
GTGGCGCGTG CCGACCAGCG CAAGGACAAG CTGGGCAACT CCCACGACCT GCAGCGCTTC 
CTCTCCGACT TCAAGGACCT GATGTCCTGG ATTAACGGCA TCCGAAGCTT GGTCTCCTCA 
GAGGAACTGG CCAAAGATGT GACCGGGGCT GAGGCCCTGC TGGAGAGACA CCAGGAGCAC 
CGTACTGAGA TCGACGCCCG TGCGGGGACC TTCCAGGCCT TTGAGCAGTT TGGCCAGCAG 
CTGTTGGCAC GCGGCCACTA TGCCAGTCCG GAGATCCAGC AGAAGCTGGA GGCCCTGGAC 
CACGAGAGGG CCGACCTGGA GAAGGCCTGG GTCCAAAGAC GCATGATGCT CGACCAGTGC 
CTGGAGCTCC AGCTGTTCAA CCGTGACTGT GAGCAGGCTG AGAACTGGAT GGCAGCGCGC 
GAGGCCTTCC TGGCCAGCGA CGACAAGGGC GACTCCCTGG ACAGCGTGGA GGCCCTCATC 
AAGAAACACG AGGACTTTGA CAAGGCCATC AATGTGCAGG AGGAGAAGAT CGCTGCCCTG 
CAGTCCTTTG CTGACCAGCT GGTCGGCGCT GACCACTACG CCAAGCCTGA GATCTCCAAA 
CGTCGCAACG AAGTCCTGGA CAGGTGGCGC CGTCTGAAGG CCCAGATGAT TGAGAAGCGT 
TCCAAGCTGG GCGAGTCCCA GACCCTGCAG CAGTTCAGCC GTGATGTGGA TGAGATTGAG 
GCCTGGATCA GCGAGAAGCT GCAGACGGCC ACCGACGAGT CCTACAAGGA CCCCACCAAC 
ATCCAGTTGT CCAAGCTGCT GAGTAAGCAC CAGAAGCACC AGGCCTTCGA GGCGGAGCTG 
CACGCCAACG CAGACCGCAT CCGTGGGGTC ATCGACACAG GAAACGCCCT CATCCAGAGA 
GGGGCCTGCG CCGGCAGTGA GGACGCAGTT CAGTCGCGTC TCGGTGCCCT GGATGAGCAG 
TGGCAGTTCC TGGTGAACAA GTCAGCAGAG AAGAGCCAGA AGCTCAAGGA AGCCAACAAG 
CAGCAGAACT TCAACACTGG CATCAAGGAC TTTGACTTCT GGCTATCTGA GGTCGAGGCC 
CTTCTTGCCT CTGAGGACTA TGGCAAAGAC CTGGCCTCCG TCAACAACCT GCTGAAGAAA 
CACCAGCTTC TGGAAGCCGA CATCTCTGCT CATGAGGATC GCCTGAAGGA CCTGAACGGC 
CAGGCCGACA GCCTGACGGC CAGCACAGCC TTCGACCCCA CCCAGGTCAA GGACAAGCGC 
AACGCCGTCA ACGGACGCTT CGCCAAGATC AAGAGCATGG CTGCCGGCCG CCGCGCCAAG 
CTCAACGAAT CTCACCGCCT GCACCAGTTC TTCAGGGACC TGGACGATGA GGAGTCTTGG 
ATAAAAGAAA AGAAATTGCT GGTGAGTTCG GAAGACTATG GACGTGATTT GACAGGAGTG 
CAGAATCTAA GGAAGAAACA CAAGAGGCTG GAGGCTGAGC TGGGGGCCCA CGAGCCAGCC 
ATCCAGTCTG TGCAGGACAC TGGGAAGAAG CTGTCTGATG ACAACACCAT CGGCCAGGAG 
GAGATTGAGC AGAGGCTGGG CCAGTTTGAG GAGCACTGGA CGGAGCTGAA GGACCTGGCT 
GCAGCAAGGG GACAGAGGCT GGAGGAGTCG CTGGAGTACC AGCAGTTTGT TGCGAATGTT 
GAAGAGGAGG AAGCCTGGAT TAACGAGAAG CTGAACCTGG TGGGAAGTGA AGACTATGGG 
GATACCCTGG CCGCCGTGCA GGGCCTGTTG AAGAAGCATG AGGCTTTTGA GACTGACTTC 
ACCGTGCACA GGGACAGAGT GAATGATGTG TGTTCCAACG GAGACGAGCT CATCAACAAG 
AACAACCACC ACGTGGACAG CATCTCAGCC AAGATGACGT CCCTGCGGGG CAAGGTGTCT 
GAGCTGGAGA GGGCCGCTGC CATGAGGAAA GCCAAGCTGG ACGAGAACTC TGCCTTCCTG 
CAGTTCAACT GGAAGGCCGA CGTGGTGGAG TCCTGGATCG GTGAGAAGGA GAACAGCCTG 
AAGACTGATG ACTACGGACG AGATCTCTCC TCTGTGCAGA CGTTGCTCAC TAAGCAGGAG 
ACGTTTGACG CTGGTCTTCA GGCCTTCCAG CAGGAGGGCA TAACCAACAT CACGGCCCTC 
AAGGACCAGC TGCTGGCGGC AAAGCACGTC CAGTCCAAGG CCATTGAGGC CCGCCACGCC 
GCCCTCATGA AGCGCTGGAA CAAGCTGCTA GACAACTCAC AGGCTCGCAA GAAGAAGCTG 
CTGGAGGCCC AGGATCACTT CAGGAAGGTG GAAGACCTGT TCCTGACCTT TGCCAAGAAG 
GCATCGGCCT TCAATAGCTG GTTTGAGAAT GCAGAGGAGG ACCTGACGGA CCCGGTGCGC 
TGCAACTCTC TGGAGGAGAT CCGGGCTCTG CGTGACGCCC ACGAGGCCTT CCGCTCGTCG 
CTGAGCTCGG CGCAGGCCGA CTTCAACCAG CTAGCTGAGC TGGACCGGCA GATTAAGAGT 
TACCAGGTGG TGTCCAACCC CTACACCTGG TTCACCATGG AGGCCCTGGA GGAGACCTGG 
AGAAACCTGC AGAAGATCAT CAAGGAACGA GAGCTAGAGT TGCAGAAGGA GCAGAGAAGG 
CAGGAGGAGA ACGACAAGCT GCGGCAGGAG TTTGCTCAGC ATGCCAACGC ATTCCACCAG 
TGGCTGCAGG AGACCAGGAC ATATCTTCTG GATGGGTCCT GTATGGTAGA AGAGTCCGGA 
ACCTTGGAAT CACAGCTTGA GGCAACCAAG CGTAAGCACC AGGAGATCCG GGCCATGCGC 
AGCCAGCTGA AGAAGATAGA GGACTTGGGA GCGGCCATGG AGGAAGCCCT GATCCTGGAC 
AACAAGTACA CAGAACACAG CACGGTGGGC CTGGCCCAGC AGTGGGACCA GCTAGACCAA 
CTAGGAATGA GGATGCAGCA CAACCTGGAG CAGCAGATCC AGGCCAGGAA CACCACTGGA 
GTGACAGAGG ATGCCCTGAA GGAGTTCAGC ATGATGTTCA AGCATTTTGA CAAAGAGAAG 
TCTGGCCGTT TAAACCATCA GGAGTTCAAG TCGTGTCTGC GCTCGCTGGG TTACGACCTG 
CCAATGGTGG AGGAGGGAGA ACCAGACCCC GAGTTTGAGT CCATCCTCGA CACTGTCGAT 
CCCAACAGGG ATGGCAACGT GTCCTTGCAG GAGTACATGG CTTTCATGAT CAGCCGCGAA 
ACAGAGAACG TAAAGTCCAG CGAGGAGATT GAGAGCGCCT TCCGAGCCCT CAGCGTCGAT 
GCCAAGCCCT ACGTCACCAA GGAGGAGCTC TACCAGAACC TGAGCAAGGA ACAGGCAGAC 
TACTGCATCT CGCACATGAA GCCCTACCTA GACAGCAAGG GCCGGGAAAT GCCATCTGCC 
TTCGACTTTG TTGAATTCAC CCGCTCGCTT TTCGTCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021446058.1
CDS	153..7634
Translation	MATIEIQDLGQQRETSRLPRETLATTSPQRMDTSGVKVLESADDIQERRQQVLDRYRRFKELSGVRRQKLEDSYRFQFFRRDADELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANAGAIIKLDETGNLMTSEGHFASETIRTRLEELHRLWDLLLQKTKEKGVRLLQAQKLVQYLRECEDALDWISDKEAMATSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVNQLAGKLSQESHPEAELIVRKQEEVNAAWQRLKGLAQQRQGRLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVNTLGGEAERLQQTHPQNASQIHLKRDELITNWEQIRTLAAERHAHLNDSYRLQRYTADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKATDEAGQALLNTGHYALEEVKEKLGVLTEEKESLLELWEVRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAKEDVATRRDALLSRRNALHERAQSRRAALEDSFHLQQFFRDSDELKSWINEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALQKSGQELLDGKHYAADEVSVRMDEVSSQWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTSVQNLQKKHALLEADVAAHQDRIDGITIQARQFQEAGHFDGDNIKRKQEALVARYDALRDPMAARKQKLSDSLRLQQLFRDVEDEETWVREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGEAMVEEGHFAGEEVKVKLGELNGRWDTLKGKAGQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSPDYGKDEDSAEALLKKHEALMSDLSAYGTSIQALKEQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPTQSSSRENLLDEQGSIGLRQDQIENQPGAKEACSVSVRMKQVEELYGTLLELGEKRKDMLEKSCKKFMLFREANELQQWINEKEGALTNEEVGSDLEQVEVLQKKFDDFQKDLKANESRLRDINKVASELESEGLMAEEAHMVQAQEQVMVGSVPGKDDTDSKNASPWKELNNRWRSLQQLAEERSNMLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVKSLGETAERLIQSHPEAVDDIQEKCTELNTAWSSLVARADQRKDKLGNSHDLQRFLSDFKDLMSWINGIRSLVSSEELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLARGHYASPEIQQKLEALDHERADLEKAWVQRRMMLDQCLELQLFNRDCEQAENWMAAREAFLASDDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLVGADHYAKPEISKRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTATDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDTGNALIQRGACAGSEDAVQSRLGALDEQWQFLVNKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNGQADSLTASTAFDPTQVKDKRNAVNGRFAKIKSMAAGRRAKLNESHRLHQFFRDLDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELGAHEPAIQSVQDTGKKLSDDNTIGQEEIEQRLGQFEEHWTELKDLAAARGQRLEESLEYQQFVANVEEEEAWINEKLNLVGSEDYGDTLAAVQGLLKKHEAFETDFTVHRDRVNDVCSNGDELINKNNHHVDSISAKMTSLRGKVSELERAAAMRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGITNITALKDQLLAAKHVQSKAIEARHAALMKRWNKLLDNSQARKKKLLEAQDHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIRALRDAHEAFRSSLSSAQADFNQLAELDRQIKSYQVVSNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWLQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEDALKEFSMMFKHFDKEKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFESILDTVDPNRDGNVSLQEYMAFMISRETENVKSSEEIESAFRALSVDAKPYVTKEELYQNLSKEQADYCISHMKPYLDSKGREMPSAFDFVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_021590383.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss spectrin alpha chain, non-erythrocytic 1-like (LOC110509489), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021590383.1

ORF Insert Sequence:

ATGGCCACCA TTGAAATCCA AGACCTAGGG CAACAGCGGG AGACAAGTCG TCTGCCTAGG 
GAGACTTTGG CCACCACCTC TCCTCAGAGA ATGGATACCA GTGGGGTGAA AGTGCTGGAG 
TCTGCCGATG ACATCCAGGA GCGCCGGCAG CAGGTGTTGG ATCGCTACCG GCGCTTCAAG 
GAGCTGTCGG GTGTGCGCAG GCAGAAGCTG GAGGACTCCT ACCGTTTCCA GTTCTTCCGC 
CGCGATGCCG ATGAGCTGGA GAAGTGGATC CAGGAGAAGC TGCAGATCGC CTCCGATGAG 
AACTACAAGG ACCCCACCAA CCTCCAGGGC AAGCTCCAGA AACACCAGGC CTTCGAGGCG 
GAGGTGCAGG CCAACGCTGG AGCCATCATC AAGCTTGATG AGACTGGCAA CCTCATGACC 
TCAGAGGGTC ACTTTGCCTC TGAAACCATC CGCACTCGTC TGGAGGAGCT GCATCGTCTT 
TGGGACCTGC TGCTGCAGAA GACCAAGGAG AAAGGTGTTC GTCTGCTGCA GGCCCAGAAG 
CTGGTGCAGT ACCTGCGCGA GTGTGAGGAT GCCCTGGACT GGATTAGTGA CAAGGAGGCC 
ATGGCCACCT CGGAGGAGCT GGGCCAGGAC TTGGAGCATG TGGAGGTGCT CCAGAAGAAG 
TTTGAGGAGT TCCAGACGGA CCTGGCGGCT CACGAGGAGC GTGTGAACGA GGTGAACCAG 
CTGGCGGGCA AGCTGAGCCA GGAGTCCCAC CCCGAGGCGG AGCTCATCGT CCGCAAGCAG 
GAGGAGGTGA ACGCCGCCTG GCAGAGGCTC AAGGGCCTGG CACAGCAGAG GCAGGGCAGG 
CTGTTCGGAG CCGCCGAGGT GCAGCGCTTC AACAGGGATG TGGACGAGAC CATCAGCTGG 
ATCAAGGAGA AGGAGCAACT CATGGCGTCC GATGACTTTG GCCGGGACCT GGCCAGCGTT 
CAGGCCCTGC TGCGCAAGCA CGAGGGCCTG GAGAGAGACC TGGCTGCCCT GGAGGACAAG 
GTCAACACTC TGGGCGGAGA GGCTGAACGT CTGCAGCAGA CCCACCCCCA GAACGCTTCC 
CAGATCCACC TGAAGAGGGA CGAGCTCATC ACCAACTGGG AGCAGATCCG CACGCTGGCC 
GCCGAGCGCC ACGCCCACCT CAATGACTCC TACAGGCTGC AGCGCTACAC TGCAGACTTC 
CGTGACCTGA CCAGCTGGGT GACGGAGATG AAGGCCCTGA TCAACGCTGA CGAGCTGGCC 
AACGACGTGG CCGGAGCCGA GGCTCTTCTC GACCGCCACC AGGAGCACAA GGGTGAGATT 
GATGCCCATG AAGACAGCTT CAAAGCCACG GACGAAGCCG GTCAGGCCTT GCTCAACACC 
GGACACTACG CCTTAGAGGA GGTCAAGGAG AAGCTGGGTG TCCTGACTGA AGAGAAGGAG 
TCTCTGCTGG AGCTGTGGGA GGTGCGCAGG CAGCAGTACG AGCAGTGCAT GGACCTGCAG 
CTCTTCTACA GGGACACAGA GCAGGTTGAC AACTGGATGA GCAAGCAAGA GGCTTTCCTT 
CTGAACGAGG ACCTTGGTGA CTCTCTGGAC AGCGTGGAGG CCTTGCTTAA GAAACACGAG 
GACTTTGAGA AGTCCCTCAG CGCCCAGGAG GAGAAGATCA CTGCCCTGGA TGAATTTGCC 
ACCAAACTGA TCCAGAACAA CCACTATGCC AAGGAGGATG TTGCTACTCG TAGAGATGCT 
CTGCTGAGCC GCCGCAACGC CCTGCACGAG CGTGCTCAGT CCCGCCGCGC CGCTCTGGAG 
GACTCCTTCC ACCTGCAGCA GTTCTTCCGT GACTCGGACG AACTCAAGAG CTGGATCAAT 
GAGAAGATGA AGACGGCCAC CGACGAAGCC TACAAGGACC CGTCCAACCT GCAGGGCAAG 
GTCCAGAAGC ACCAGGCCTT CGAGGCGGAG CTGTCAGCCA ACCAGAGCCG CATCGATGCT 
CTGCAGAAGT CTGGCCAGGA GCTCCTAGAC GGGAAGCACT ACGCCGCCGA CGAGGTGTCT 
GTACGCATGG ACGAGGTCAG CTCCCAGTGG AAGAAGCTGC TGGAGGCCAC CGAACTCAAA 
GGCATCAAGC TGCGCGAGGC CAACCAGCAG CAGCAGTTCA ACAGAAACGT GGAGGACATT 
GAGCTGTGGC TGTATGAGGT TGAGGGCCAC CTGGCTTCAG ATGACTACGG CAAGGATCTG 
ACCAGCGTCC AGAACCTGCA GAAGAAGCAC GCCCTGCTGG AGGCTGACGT GGCTGCCCAC 
CAGGACCGCA TCGATGGCAT CACCATTCAG GCGCGTCAGT TCCAGGAAGC CGGTCACTTT 
GACGGCGACA ACATCAAGCG CAAGCAGGAG GCGCTGGTGG CGCGCTACGA TGCCCTCCGC 
GACCCCATGG CCGCCCGCAA GCAGAAGCTG TCAGACTCCC TGCGGCTGCA GCAGCTCTTC 
AGGGACGTGG AGGACGAGGA AACCTGGGTC CGGGAGAAGG AGCCCATCGC CGCCTCCACC 
AACCGGGGAA AAGACCTGAT CGGCGTCCAG AACTTGCTGA AGAAGCACCA GGCCCTGCAG 
GCGGAGATCG CTGGCCATGA GCCCCGCATC AAGGCCGTCA CCCAGAAAGG AGAGGCTATG 
GTGGAGGAAG GCCACTTTGC TGGGGAGGAG GTTAAGGTGA AGCTGGGGGA GCTGAACGGC 
CGCTGGGACA CCCTGAAAGG CAAGGCCGGG CAGCGCAGGC AGGACCTGGA GGACTCATTG 
CAGGCACAGC AGTACTTCGC CGACGCCAAC GAGGCAGAGT CCTGGATGAG GGAGAAAGAG 
CCCATCGTGG GCAGTCCCGA CTACGGCAAG GATGAGGATT CGGCTGAGGC CCTGCTGAAG 
AAGCACGAAG CCCTGATGTC TGACCTGAGT GCTTATGGCA CCAGCATCCA GGCTTTGAAG 
GAGCAGGCCC AATCCTGCAG GCAACAAGTT GCGCCCACCG ATGATGAGAC TGGCAAGGAG 
CTGGTGCTGG CCCTCTATGA CTACCAGGAG AAGAGCCCCC GTGAGGTCAC CATGAAGAAG 
GGAGACATCC TCACCCTGCT CAACAGTACC AACAAGGACT GGTGGAAGGT GGAGGTGAAC 
GACCGCCAGG GCTTTGTGCC AGCAGCCTAC GTCAAGAAGC TAGACCCCAC CCAGTCCTCC 
TCCAGGGAGA ACCTGCTGGA CGAGCAGGGC AGCATCGGCC TCCGCCAGGA CCAGATCGAG 
AACCAGCCGG GCGCAAAGGA GGCGTGCAGT GTGTCTGTAC GCATGAAGCA GGTGGAGGAA 
CTGTACGGCA CTCTCCTGGA GCTGGGCGAG AAACGCAAGG ACATGCTAGA AAAGAGCTGC 
AAGAAGTTCA TGCTGTTCCG CGAGGCTAAC GAACTGCAGC AGTGGATCAA CGAGAAGGAG 
GGAGCCCTCA CCAACGAGGA GGTGGGCTCT GACCTGGAGC AGGTGGAGGT GCTGCAGAAG 
AAGTTTGACG ACTTCCAGAA GGACCTGAAG GCCAACGAGT CTCGTCTGAG GGACATCAAC 
AAGGTGGCAT CTGAGCTGGA GTCTGAGGGC CTAATGGCAG AGGAGGCACA CATGGTCCAA 
GCTCAGGAAC AAGTAATGGT GGGTTCCGTT CCTGGCAAGG ATGATACCGA TTCCAAAAAT 
GCTTCACCAT GGAAGGAGCT GAACAACCGC TGGAGGTCCC TGCAGCAGCT GGCTGAGGAG 
AGGAGCAACA TGCTGGGGAG TGCCCATGAG GTGCAGAGGT TCCACAGGGA TGCGGATGAG 
ACCAAGGAGT GGATCGAGGA GAAGAACCAG GCCCTGAACA CCGACAACTA CGGCCACGAC 
CTGGCTAGCG TTCAGGCTCT GCAGCGCAAA CATGAGGGCT TTGAGAGAGA CCTGGCCGCC 
TTGGGAGACA AGGTGAAGTC TCTGGGGGAG ACGGCGGAGC GTCTGATCCA GTCCCACCCG 
GAGGCTGTGG ACGACATCCA GGAGAAGTGC ACAGAGCTCA ACACGGCCTG GAGCAGCCTA 
GTGGCGCGTG CCGACCAGCG CAAGGACAAG CTGGGCAACT CCCACGACCT GCAGCGCTTC 
CTCTCCGACT TCAAGGACCT GATGTCCTGG ATTAACGGCA TCCGAAGCTT GGTCTCCTCA 
GAGGAACTGG CCAAAGATGT GACCGGGGCT GAGGCCCTGC TGGAGAGACA CCAGGAGCAC 
CGTACTGAGA TCGACGCCCG TGCGGGGACC TTCCAGGCCT TTGAGCAGTT TGGCCAGCAG 
CTGTTGGCAC GCGGCCACTA TGCCAGTCCG GAGATCCAGC AGAAGCTGGA GGCCCTGGAC 
CACGAGAGGG CCGACCTGGA GAAGGCCTGG GTCCAAAGAC GCATGATGCT CGACCAGTGC 
CTGGAGCTCC AGCTGTTCAA CCGTGACTGT GAGCAGGCTG AGAACTGGAT GGCAGCGCGC 
GAGGCCTTCC TGGCCAGCGA CGACAAGGGC GACTCCCTGG ACAGCGTGGA GGCCCTCATC 
AAGAAACACG AGGACTTTGA CAAGGCCATC AATGTGCAGG AGGAGAAGAT CGCTGCCCTG 
CAGTCCTTTG CTGACCAGCT GGTCGGCGCT GACCACTACG CCAAGCCTGA GATCTCCAAA 
CGTCGCAACG AAGTCCTGGA CAGGTGGCGC CGTCTGAAGG CCCAGATGAT TGAGAAGCGT 
TCCAAGCTGG GCGAGTCCCA GACCCTGCAG CAGTTCAGCC GTGATGTGGA TGAGATTGAG 
GCCTGGATCA GCGAGAAGCT GCAGACGGCC ACCGACGAGT CCTACAAGGA CCCCACCAAC 
ATCCAGTTGT CCAAGCTGCT GAGTAAGCAC CAGAAGCACC AGGCCTTCGA GGCGGAGCTG 
CACGCCAACG CAGACCGCAT CCGTGGGGTC ATCGACACAG GAAACGCCCT CATCCAGAGA 
GGGGCCTGCG CCGGCAGTGA GGACGCAGTT CAGTCGCGTC TCGGTGCCCT GGATGAGCAG 
TGGCAGTTCC TGGTGAACAA GTCAGCAGAG AAGAGCCAGA AGCTCAAGGA AGCCAACAAG 
CAGCAGAACT TCAACACTGG CATCAAGGAC TTTGACTTCT GGCTATCTGA GGTCGAGGCC 
CTTCTTGCCT CTGAGGACTA TGGCAAAGAC CTGGCCTCCG TCAACAACCT GCTGAAGAAA 
CACCAGCTTC TGGAAGCCGA CATCTCTGCT CATGAGGATC GCCTGAAGGA CCTGAACGGC 
CAGGCCGACA GCCTGACGGC CAGCACAGCC TTCGACCCCA CCCAGGTCAA GGACAAGCGC 
AACGCCGTCA ACGGACGCTT CGCCAAGATC AAGAGCATGG CTGCCGGCCG CCGCGCCAAG 
CTCAACGAAT CTCACCGCCT GCACCAGTTC TTCAGGGACC TGGACGATGA GGAGTCTTGG 
ATAAAAGAAA AGAAATTGCT GGTGAGTTCG GAAGACTATG GACGTGATTT GACAGGAGTG 
CAGAATCTAA GGAAGAAACA CAAGAGGCTG GAGGCTGAGC TGGGGGCCCA CGAGCCAGCC 
ATCCAGTCTG TGCAGGACAC TGGGAAGAAG CTGTCTGATG ACAACACCAT CGGCCAGGAG 
GAGATTGAGC AGAGGCTGGG CCAGTTTGAG GAGCACTGGA CGGAGCTGAA GGACCTGGCT 
GCAGCAAGGG GACAGAGGCT GGAGGAGTCG CTGGAGTACC AGCAGTTTGT TGCGAATGTT 
GAAGAGGAGG AAGCCTGGAT TAACGAGAAG CTGAACCTGG TGGGAAGTGA AGACTATGGG 
GATACCCTGG CCGCCGTGCA GGGCCTGTTG AAGAAGCATG AGGCTTTTGA GACTGACTTC 
ACCGTGCACA GGGACAGAGT GAATGATGTG TGTTCCAACG GAGACGAGCT CATCAACAAG 
AACAACCACC ACGTGGACAG CATCTCAGCC AAGATGACGT CCCTGCGGGG CAAGGTGTCT 
GAGCTGGAGA GGGCCGCTGC CATGAGGAAA GCCAAGCTGG ACGAGAACTC TGCCTTCCTG 
CAGTTCAACT GGAAGGCCGA CGTGGTGGAG TCCTGGATCG GTGAGAAGGA GAACAGCCTG 
AAGACTGATG ACTACGGACG AGATCTCTCC TCTGTGCAGA CGTTGCTCAC TAAGCAGGAG 
ACGTTTGACG CTGGTCTTCA GGCCTTCCAG CAGGAGGGCA TAACCAACAT CACGGCCCTC 
AAGGACCAGC TGCTGGCGGC AAAGCACGTC CAGTCCAAGG CCATTGAGGC CCGCCACGCC 
GCCCTCATGA AGCGCTGGAA CAAGCTGCTA GACAACTCAC AGGCTCGCAA GAAGAAGCTG 
CTGGAGGCCC AGGATCACTT CAGGAAGGTG GAAGACCTGT TCCTGACCTT TGCCAAGAAG 
GCATCGGCCT TCAATAGCTG GTTTGAGAAT GCAGAGGAGG ACCTGACGGA CCCGGTGCGC 
TGCAACTCTC TGGAGGAGAT CCGGGCTCTG CGTGACGCCC ACGAGGCCTT CCGCTCGTCG 
CTGAGCTCGG CGCAGGCCGA CTTCAACCAG CTAGCTGAGC TGGACCGGCA GATTAAGAGT 
TACCAGGTGG TGTCCAACCC CTACACCTGG TTCACCATGG AGGCCCTGGA GGAGACCTGG 
AGAAACCTGC AGAAGATCAT CAAGGAACGA GAGCTAGAGT TGCAGAAGGA GCAGAGAAGG 
CAGGAGGAGA ACGACAAGCT GCGGCAGGAG TTTGCTCAGC ATGCCAACGC ATTCCACCAG 
TGGCTGCAGG AGACCAGGAC ATATCTTCTG GATGGGTCCT GTATGGTAGA AGAGTCCGGA 
ACCTTGGAAT CACAGCTTGA GGCAACCAAG CGTAAGCACC AGGAGATCCG GGCCATGCGC 
AGCCAGCTGA AGAAGATAGA GGACTTGGGA GCGGCCATGG AGGAAGCCCT GATCCTGGAC 
AACAAGTACA CAGAACACAG CACGGTGGGC CTGGCCCAGC AGTGGGACCA GCTAGACCAA 
CTAGGAATGA GGATGCAGCA CAACCTGGAG CAGCAGATCC AGGCCAGGAA CACCACTGGA 
GTGACAGAGG ATGCCCTGAA GGAGTTCAGC ATGATGTTCA AGCATTTTGA CAAAGAGAAG 
TCTGGCCGTT TAAACCATCA GGAGTTCAAG TCGTGTCTGC GCTCGCTGGG TTACGACCTG 
CCAATGGTGG AGGAGGGAGA ACCAGACCCC GAGTTTGAGT CCATCCTCGA CACTGTCGAT 
CCCAACAGGG ATGGCAACGT GTCCTTGCAG GAGTACATGG CTTTCATGAT CAGCCGCGAA 
ACAGAGAACG TAAAGTCCAG CGAGGAGATT GAGAGCGCCT TCCGAGCCCT CAGCGTCGAT 
GCCAAGCCCT ACGTCACCAA GGAGGAGCTC TACCAGAACC TGAGCAAGGA ACAGGCAGAC 
TACTGCATCT CGCACATGAA GCCCTACCTA GACAGCAAGG GCCGGGAAAT GCCATCTGCC 
TTCGACTTTG TTGAATTCAC CCGCTCGCTT TTCGTCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC110509489 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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