SPTBN1 cDNA ORF clone, Chlorocebus sabaeus(green monkey)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPTBN1
Entrez Gene ID	103220257
Full Name	spectrin beta, non-erythrocytic 1
Gene Type	protein-coding
Organism	Chlorocebus sabaeus(green monkey)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007970629.1	XP_007968820.1	spectrin beta chain, non-erythrocytic 1 isoform X1
XM_007970631.1	XP_007968822.1	spectrin beta chain, non-erythrocytic 1 isoform X3
XM_007970628.1	XP_007968819.1	spectrin beta chain, non-erythrocytic 1 isoform X1
XM_007970630.1	XP_007968821.1	spectrin beta chain, non-erythrocytic 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following SPTBN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTBN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCh102613	XM_007970629.1 Latest version!	Chlorocebus sabaeus spectrin, beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCh102613	XM_007970628.1 Latest version!	Chlorocebus sabaeus spectrin, beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCh102614	XM_007970630.1 Latest version!	Chlorocebus sabaeus spectrin, beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCh102615	XM_007970631.1 Latest version!	Chlorocebus sabaeus spectrin, beta, non-erythrocytic 1 (SPTBN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPTBN1 ( XM_007970629.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007968820.1 Chlorocebus sabaeus spectrin, beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCh102613
Clone ID Related Accession (Same CDS sequence)	XM_007970629.1 , XM_007970628.1
Accession Version	XM_007970629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	spectrin beta chain, non-erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735260.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
GTCAACAACC GCTGGGATGT TGATGACTGG GACAATGAAA ACAGCTCTGC GCGGCTCTTT 
GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTACAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTTGCCCGA GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAAGGACG AATGCGCATC CACTGCCTAG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GATCCCATGA CATCGTGGAT 
GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAACC TGCAGAATGC ATTTAATCTG 
GCAGAGCAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAC 
CCTGATGAGA AGTCCATAAT CACTTACGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCTTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGATAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA 
ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG 
CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC 
TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCAGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGTGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCCGCAGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT 
GTGGTAGCCG TGGCCAGGGA GCTCGAGGCT GAGAATTACC ACGACATCAA GCGCATCACA 
GCAAGGAAGG ACAATGTCAT CCGACTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG 
AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGACGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCAGAGCGGG TGAGAGGTGT CAATGCCTCT GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GCGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT 
CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCAG ACGATTATGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC 
CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCACT TCGAGCAGGC CATCAAGGAA 
GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCAGAGA AGATCCGTGA GAGGATCATT 
TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCAG CCATTCGGAA GAAGCGCCTG 
GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ACGACATTGA TGCCTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAGC GATGTGGGCC ACGATGAATA TTCCACACAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATTG CCAATTATAG GCCCACCCTT 
GACACGCTGC ACGAGCAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGATGTG 
AGGGGCAGGC TGTCGGGCAT CGAGGAGCGA TATAAGGAGG TGGCAGAGCT GACGCGGCTG 
CGGAAGCAGG CACTCCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGCGA GGCCGACGCC 
TGTGAGCTCT GGATCGACGA AAAGGAGCAG TGGCTCAATA ACATGCAGAT CCCAGAGAAG 
CTGGAGGATC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTCGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGTGGCAAC 
CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACGAGGTG GAGCCAGTTC 
AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAACGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC 
ACCCAAGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG 
AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAAGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG 
ACCCAGACAG CAATCGCCTC GGAGGACATG CCAAACACCC TGACTGAGGC CGAGAAGCTG 
CTTACACAGC ATGAGAACAT CAAGAACGAG ATTGACAACT ATGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT AGACACTGGA TGGAACGAGC TGCACAAGAT GTGGGAGAAC 
AGACAAAATC TCCTTTCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA 
GCGGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAGGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC 
AATGAGGAGA AGATCAACGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ACGACAGACA TAGGAAGAAT 
CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGAGATCT ACAGAAATTC 
CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC GGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAACCTGTG GTGAAGGAGA AACTCACTGG TTTACATGAA 
ATGTGGGAAG TCCTTGAATC CACTACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTACGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA 
AGCCAGGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTGGA CAGCAAGCGC 
CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCC TGAACGAGAG GAAGCATAAC 
CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGATGA GATCTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGC 
ATTGATGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACCG ACAGCAGCAG CCTCAACGCT 
GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG 
ACGGAGAAAC GCCACAGGCG GCTGGAGGAG GCACACCGGG CCCAGCAGTA CTACTTTGAC 
GCTGCCGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT 
GTGGAGGACT ATGCAGAGAC TGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC 
GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC 
GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGATTA 
TTCCAGCTCA ACCGGGAGGT AGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAATTTG CCCGAGACAC CGGGAACATT GGACAGGAGC GTGTGGACAC GGTCAATCAC 
ATGGCAGATG AGCTCATCAA CTCTGGCCAT TCAGATGCCG CCACCATTGC TGAATGGAAG 
GACGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT 
CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT 
ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCAGCCT ATGCGGGTGA CAAGGCCGAC 
GACATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTGTT GGACGCCTGC 
GAGAGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTG 
CGTGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT 
GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCAAGA 
AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT 
CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGCCAGGA GCCGTACCTG 
TCCAGCCGAG AAATAGGCCA GAGTGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCATTTGAAA AGTCTGCAGC GACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT 
ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCTG AGTCCCAGCA GCAGTGGGAT 
ACTGCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCGG CTGAACAGGG ATCTCCACGG 
ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGAACGAGC 
TCTAAAGAGT CCAGCCCCAT CCCCTCCCCA ACCTCTGATC GCAAAGCCAA GACTGCCCTC 
CCAACCCAGA GTGCGGCCAC CTTACCAGCC AGAACCCAGG AGACGCCTTC GGCCCAGATG 
GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT 
GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT 
GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG 
GCCATCTCTT CGGCCATCTC CTCTGATAAA CATGAGGTGT CTGCCAGCAC CCAGAGCACG 
CCAGCATCCA GCCGGGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC 
AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT 
GGCAAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007968820.1
CDS	129..7223
Translation	MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLVLSQDYGKHLLGVEDLLQKHTLVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERIIYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSSDVGHDEYSTQSLVKKHKDVAEEIANYRPTLDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGNPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNRETASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEPVVKEKLTGLHEMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLNAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHMADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACESRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKVSEEAESQQQWDTAKGEQVSQNGLPAEQGSPRMAETVDTSEMVNGATEQRTSSKESSPIPSPTSDRKAKTALPTQSAATLPARTQETPSAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKTAASGIPYHSEVPVSLKEAVCEVALDYKKKKHVFKLRLNDGNEYLFQAKDDEEMNTWIQAISSAISSDKHEVSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSLFGKKK

Target ORF information:

RefSeq Version	XM_007970629.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus spectrin, beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007970629.1

ORF Insert Sequence:

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
GTCAACAACC GCTGGGATGT TGATGACTGG GACAATGAAA ACAGCTCTGC GCGGCTCTTT 
GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTACAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTTGCCCGA GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAAGGACG AATGCGCATC CACTGCCTAG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GATCCCATGA CATCGTGGAT 
GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAACC TGCAGAATGC ATTTAATCTG 
GCAGAGCAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAC 
CCTGATGAGA AGTCCATAAT CACTTACGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCTTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGATAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA 
ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG 
CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC 
TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCAGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGTGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCCGCAGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT 
GTGGTAGCCG TGGCCAGGGA GCTCGAGGCT GAGAATTACC ACGACATCAA GCGCATCACA 
GCAAGGAAGG ACAATGTCAT CCGACTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG 
AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGACGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCAGAGCGGG TGAGAGGTGT CAATGCCTCT GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GCGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT 
CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCAG ACGATTATGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC 
CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCACT TCGAGCAGGC CATCAAGGAA 
GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCAGAGA AGATCCGTGA GAGGATCATT 
TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCAG CCATTCGGAA GAAGCGCCTG 
GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ACGACATTGA TGCCTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAGC GATGTGGGCC ACGATGAATA TTCCACACAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATTG CCAATTATAG GCCCACCCTT 
GACACGCTGC ACGAGCAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGATGTG 
AGGGGCAGGC TGTCGGGCAT CGAGGAGCGA TATAAGGAGG TGGCAGAGCT GACGCGGCTG 
CGGAAGCAGG CACTCCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGCGA GGCCGACGCC 
TGTGAGCTCT GGATCGACGA AAAGGAGCAG TGGCTCAATA ACATGCAGAT CCCAGAGAAG 
CTGGAGGATC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTCGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGTGGCAAC 
CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACGAGGTG GAGCCAGTTC 
AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAACGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC 
ACCCAAGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG 
AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAAGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG 
ACCCAGACAG CAATCGCCTC GGAGGACATG CCAAACACCC TGACTGAGGC CGAGAAGCTG 
CTTACACAGC ATGAGAACAT CAAGAACGAG ATTGACAACT ATGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT AGACACTGGA TGGAACGAGC TGCACAAGAT GTGGGAGAAC 
AGACAAAATC TCCTTTCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA 
GCGGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAGGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC 
AATGAGGAGA AGATCAACGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ACGACAGACA TAGGAAGAAT 
CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGAGATCT ACAGAAATTC 
CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC GGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAACCTGTG GTGAAGGAGA AACTCACTGG TTTACATGAA 
ATGTGGGAAG TCCTTGAATC CACTACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTACGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA 
AGCCAGGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTGGA CAGCAAGCGC 
CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCC TGAACGAGAG GAAGCATAAC 
CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGATGA GATCTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGC 
ATTGATGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACCG ACAGCAGCAG CCTCAACGCT 
GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG 
ACGGAGAAAC GCCACAGGCG GCTGGAGGAG GCACACCGGG CCCAGCAGTA CTACTTTGAC 
GCTGCCGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT 
GTGGAGGACT ATGCAGAGAC TGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC 
GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC 
GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGATTA 
TTCCAGCTCA ACCGGGAGGT AGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAATTTG CCCGAGACAC CGGGAACATT GGACAGGAGC GTGTGGACAC GGTCAATCAC 
ATGGCAGATG AGCTCATCAA CTCTGGCCAT TCAGATGCCG CCACCATTGC TGAATGGAAG 
GACGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT 
CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT 
ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCAGCCT ATGCGGGTGA CAAGGCCGAC 
GACATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTGTT GGACGCCTGC 
GAGAGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTG 
CGTGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT 
GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCAAGA 
AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT 
CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGCCAGGA GCCGTACCTG 
TCCAGCCGAG AAATAGGCCA GAGTGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCATTTGAAA AGTCTGCAGC GACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT 
ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCTG AGTCCCAGCA GCAGTGGGAT 
ACTGCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCGG CTGAACAGGG ATCTCCACGG 
ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGAACGAGC 
TCTAAAGAGT CCAGCCCCAT CCCCTCCCCA ACCTCTGATC GCAAAGCCAA GACTGCCCTC 
CCAACCCAGA GTGCGGCCAC CTTACCAGCC AGAACCCAGG AGACGCCTTC GGCCCAGATG 
GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT 
GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT 
GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG 
GCCATCTCTT CGGCCATCTC CTCTGATAAA CATGAGGTGT CTGCCAGCAC CCAGAGCACG 
CCAGCATCCA GCCGGGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC 
AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT 
GGCAAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTBN1 ( XM_007970628.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007968819.1 Chlorocebus sabaeus spectrin, beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA.

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CloneID	OCh102613
Clone ID Related Accession (Same CDS sequence)	XM_007970629.1 , XM_007970628.1
Accession Version	XM_007970628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	spectrin beta chain, non-erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735260.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
GTCAACAACC GCTGGGATGT TGATGACTGG GACAATGAAA ACAGCTCTGC GCGGCTCTTT 
GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTACAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTTGCCCGA GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAAGGACG AATGCGCATC CACTGCCTAG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GATCCCATGA CATCGTGGAT 
GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAACC TGCAGAATGC ATTTAATCTG 
GCAGAGCAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAC 
CCTGATGAGA AGTCCATAAT CACTTACGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCTTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGATAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA 
ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG 
CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC 
TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCAGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGTGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCCGCAGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT 
GTGGTAGCCG TGGCCAGGGA GCTCGAGGCT GAGAATTACC ACGACATCAA GCGCATCACA 
GCAAGGAAGG ACAATGTCAT CCGACTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG 
AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGACGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCAGAGCGGG TGAGAGGTGT CAATGCCTCT GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GCGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT 
CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCAG ACGATTATGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC 
CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCACT TCGAGCAGGC CATCAAGGAA 
GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCAGAGA AGATCCGTGA GAGGATCATT 
TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCAG CCATTCGGAA GAAGCGCCTG 
GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ACGACATTGA TGCCTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAGC GATGTGGGCC ACGATGAATA TTCCACACAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATTG CCAATTATAG GCCCACCCTT 
GACACGCTGC ACGAGCAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGATGTG 
AGGGGCAGGC TGTCGGGCAT CGAGGAGCGA TATAAGGAGG TGGCAGAGCT GACGCGGCTG 
CGGAAGCAGG CACTCCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGCGA GGCCGACGCC 
TGTGAGCTCT GGATCGACGA AAAGGAGCAG TGGCTCAATA ACATGCAGAT CCCAGAGAAG 
CTGGAGGATC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTCGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGTGGCAAC 
CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACGAGGTG GAGCCAGTTC 
AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAACGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC 
ACCCAAGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG 
AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAAGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG 
ACCCAGACAG CAATCGCCTC GGAGGACATG CCAAACACCC TGACTGAGGC CGAGAAGCTG 
CTTACACAGC ATGAGAACAT CAAGAACGAG ATTGACAACT ATGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT AGACACTGGA TGGAACGAGC TGCACAAGAT GTGGGAGAAC 
AGACAAAATC TCCTTTCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA 
GCGGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAGGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC 
AATGAGGAGA AGATCAACGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ACGACAGACA TAGGAAGAAT 
CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGAGATCT ACAGAAATTC 
CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC GGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAACCTGTG GTGAAGGAGA AACTCACTGG TTTACATGAA 
ATGTGGGAAG TCCTTGAATC CACTACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTACGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA 
AGCCAGGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTGGA CAGCAAGCGC 
CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCC TGAACGAGAG GAAGCATAAC 
CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGATGA GATCTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGC 
ATTGATGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACCG ACAGCAGCAG CCTCAACGCT 
GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG 
ACGGAGAAAC GCCACAGGCG GCTGGAGGAG GCACACCGGG CCCAGCAGTA CTACTTTGAC 
GCTGCCGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT 
GTGGAGGACT ATGCAGAGAC TGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC 
GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC 
GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGATTA 
TTCCAGCTCA ACCGGGAGGT AGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAATTTG CCCGAGACAC CGGGAACATT GGACAGGAGC GTGTGGACAC GGTCAATCAC 
ATGGCAGATG AGCTCATCAA CTCTGGCCAT TCAGATGCCG CCACCATTGC TGAATGGAAG 
GACGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT 
CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT 
ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCAGCCT ATGCGGGTGA CAAGGCCGAC 
GACATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTGTT GGACGCCTGC 
GAGAGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTG 
CGTGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT 
GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCAAGA 
AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT 
CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGCCAGGA GCCGTACCTG 
TCCAGCCGAG AAATAGGCCA GAGTGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCATTTGAAA AGTCTGCAGC GACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT 
ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCTG AGTCCCAGCA GCAGTGGGAT 
ACTGCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCGG CTGAACAGGG ATCTCCACGG 
ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGAACGAGC 
TCTAAAGAGT CCAGCCCCAT CCCCTCCCCA ACCTCTGATC GCAAAGCCAA GACTGCCCTC 
CCAACCCAGA GTGCGGCCAC CTTACCAGCC AGAACCCAGG AGACGCCTTC GGCCCAGATG 
GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT 
GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT 
GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG 
GCCATCTCTT CGGCCATCTC CTCTGATAAA CATGAGGTGT CTGCCAGCAC CCAGAGCACG 
CCAGCATCCA GCCGGGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC 
AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT 
GGCAAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007968819.1
CDS	235..7329
Translation	MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLVLSQDYGKHLLGVEDLLQKHTLVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERIIYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSSDVGHDEYSTQSLVKKHKDVAEEIANYRPTLDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGNPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNRETASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEPVVKEKLTGLHEMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLNAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHMADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACESRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKVSEEAESQQQWDTAKGEQVSQNGLPAEQGSPRMAETVDTSEMVNGATEQRTSSKESSPIPSPTSDRKAKTALPTQSAATLPARTQETPSAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKTAASGIPYHSEVPVSLKEAVCEVALDYKKKKHVFKLRLNDGNEYLFQAKDDEEMNTWIQAISSAISSDKHEVSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSLFGKKK

Target ORF information:

RefSeq Version	XM_007970628.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus spectrin, beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007970628.1

ORF Insert Sequence:

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
GTCAACAACC GCTGGGATGT TGATGACTGG GACAATGAAA ACAGCTCTGC GCGGCTCTTT 
GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTACAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTTGCCCGA GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAAGGACG AATGCGCATC CACTGCCTAG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GATCCCATGA CATCGTGGAT 
GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAACC TGCAGAATGC ATTTAATCTG 
GCAGAGCAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAC 
CCTGATGAGA AGTCCATAAT CACTTACGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCTTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGATAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA 
ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG 
CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC 
TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCAGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGTGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCCGCAGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT 
GTGGTAGCCG TGGCCAGGGA GCTCGAGGCT GAGAATTACC ACGACATCAA GCGCATCACA 
GCAAGGAAGG ACAATGTCAT CCGACTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG 
AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGACGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCAGAGCGGG TGAGAGGTGT CAATGCCTCT GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GCGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT 
CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCAG ACGATTATGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC 
CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCACT TCGAGCAGGC CATCAAGGAA 
GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCAGAGA AGATCCGTGA GAGGATCATT 
TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCAG CCATTCGGAA GAAGCGCCTG 
GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ACGACATTGA TGCCTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAGC GATGTGGGCC ACGATGAATA TTCCACACAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATTG CCAATTATAG GCCCACCCTT 
GACACGCTGC ACGAGCAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGATGTG 
AGGGGCAGGC TGTCGGGCAT CGAGGAGCGA TATAAGGAGG TGGCAGAGCT GACGCGGCTG 
CGGAAGCAGG CACTCCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGCGA GGCCGACGCC 
TGTGAGCTCT GGATCGACGA AAAGGAGCAG TGGCTCAATA ACATGCAGAT CCCAGAGAAG 
CTGGAGGATC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTCGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGTGGCAAC 
CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACGAGGTG GAGCCAGTTC 
AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAACGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC 
ACCCAAGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG 
AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAAGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG 
ACCCAGACAG CAATCGCCTC GGAGGACATG CCAAACACCC TGACTGAGGC CGAGAAGCTG 
CTTACACAGC ATGAGAACAT CAAGAACGAG ATTGACAACT ATGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT AGACACTGGA TGGAACGAGC TGCACAAGAT GTGGGAGAAC 
AGACAAAATC TCCTTTCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA 
GCGGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAGGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC 
AATGAGGAGA AGATCAACGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ACGACAGACA TAGGAAGAAT 
CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGAGATCT ACAGAAATTC 
CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC GGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAACCTGTG GTGAAGGAGA AACTCACTGG TTTACATGAA 
ATGTGGGAAG TCCTTGAATC CACTACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTACGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA 
AGCCAGGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTGGA CAGCAAGCGC 
CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCC TGAACGAGAG GAAGCATAAC 
CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGATGA GATCTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGC 
ATTGATGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACCG ACAGCAGCAG CCTCAACGCT 
GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG 
ACGGAGAAAC GCCACAGGCG GCTGGAGGAG GCACACCGGG CCCAGCAGTA CTACTTTGAC 
GCTGCCGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT 
GTGGAGGACT ATGCAGAGAC TGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC 
GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC 
GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGATTA 
TTCCAGCTCA ACCGGGAGGT AGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAATTTG CCCGAGACAC CGGGAACATT GGACAGGAGC GTGTGGACAC GGTCAATCAC 
ATGGCAGATG AGCTCATCAA CTCTGGCCAT TCAGATGCCG CCACCATTGC TGAATGGAAG 
GACGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT 
CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT 
ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCAGCCT ATGCGGGTGA CAAGGCCGAC 
GACATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTGTT GGACGCCTGC 
GAGAGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTG 
CGTGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT 
GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCAAGA 
AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT 
CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGCCAGGA GCCGTACCTG 
TCCAGCCGAG AAATAGGCCA GAGTGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCATTTGAAA AGTCTGCAGC GACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT 
ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCTG AGTCCCAGCA GCAGTGGGAT 
ACTGCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCGG CTGAACAGGG ATCTCCACGG 
ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGAACGAGC 
TCTAAAGAGT CCAGCCCCAT CCCCTCCCCA ACCTCTGATC GCAAAGCCAA GACTGCCCTC 
CCAACCCAGA GTGCGGCCAC CTTACCAGCC AGAACCCAGG AGACGCCTTC GGCCCAGATG 
GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT 
GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT 
GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG 
GCCATCTCTT CGGCCATCTC CTCTGATAAA CATGAGGTGT CTGCCAGCAC CCAGAGCACG 
CCAGCATCCA GCCGGGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC 
AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT 
GGCAAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTBN1 ( XM_007970630.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007968821.1 Chlorocebus sabaeus spectrin, beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA.

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CloneID	OCh102614
Clone ID Related Accession (Same CDS sequence)	XM_007970630.1
Accession Version	XM_007970630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	spectrin beta chain, non-erythrocytic 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735260.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTGC AGAGGACGTC TAGCATCTCC GGGCCACTGT CGCCGGCGTA CACGGGGCAG 
GTGCCTTACA ACTACAACCA GCTGGAAGGC AGATTCAAGC AGCTGCAAGA TGAGCGTGAA 
GCCGTACAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCCCG AGTGTCCTGC 
CGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAG 
GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACCAAAGGAC GAATGCGCAT CCACTGCCTA 
GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG 
GGATCCCATG ACATCGTGGA TGGAAACCAC CGGCTGACCC TTGGCCTCAT CTGGACCATC 
ATCCTGCGCT TCCAGATCCA GGATATCAGT GTGGAAACTG AAGACAACAA AGAGAAGAAA 
TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAATGTC 
AACATTCACA ATTTCACCAC TAGCTGGAGG GACGGCATGG CCTTCAATGC ACTGATACAC 
AAACACCGGC CTGACCTGAT AGATTTTGAC AAACTAAAGA AATCCAATGC ACACTACAAC 
CTGCAGAATG CATTTAATCT GGCAGAGCAG CACCTCGGCC TCACTAAACT GTTGGACCCC 
GAAGACATCA GCGTGGACCA CCCTGATGAG AAGTCCATAA TCACTTACGT GGTGACTTAT 
TACCACTACT TCTCTAAGAT GAAGGCTTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG 
CTTGATAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAATCACT TGCCTCTGAC 
CTTCTGGAAT GGATTGAACA AACCATCATC ATTCTGAACA ATCGCAAATT TGCCAATTCA 
CTGGTCGGGG TTCAACAGCA GCTTCAGGCA TTCAACACTT ACCGCACTGT GGAGAAACCA 
CCCAAATTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTCT TCACCATTCA GAGCAAGATG 
AGGGCCAACA ACCAGAAGGT CTACATGCCC CGGGAGGGGA AGCTCATCTC TGACATCAAC 
AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CACGAAAGAG AACTGGCTTT GCGGAATGAG 
CTCATAAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGATCGCAA GGCAGCTATG 
AGGGAGACTT GGCTGAGTGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGGTTTGAC 
CTTCCCGCAG TTGAGGCCGC CACAAAAAAG CACGAGGCCA TTGAGACAGA CATTGCCGCA 
TACGAGGAGC GTGTGCAGGC TGTGGTAGCC GTGGCCAGGG AGCTCGAGGC TGAGAATTAC 
CACGACATCA AGCGCATCAC AGCAAGGAAG GACAATGTCA TCCGACTCTG GGAATACCTA 
CTGGAACTGC TCAGGGCCCG GAGACAGCGG CTCGAGATGA ACCTGGGGCT GCAGAAGATA 
TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGACGAAA TGAAGGTGCT AGTATTGTCT 
CAAGACTATG GCAAACACTT ACTTGGTGTG GAAGACCTGT TACAGAAGCA CACCCTGGTT 
GAAGCAGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCCTC TGCCCAGAAG 
TTCGCAACAG ACGGGGAAGG TTACAAGCCC TGCGACCCCC AGGTGATCCG AGACCGCGTG 
GCCCACATGG AGTTCTGTTA TCAAGAGCTT TGCCAGCTGG CGGCTGAGCG CAGGGCCCGT 
CTGGAAGAGT CCCGCCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAAGGCTGG 
ATACGGGAGA AGGAGAAGAT CCTGTCCTCA GACGATTATG GGAAAGACCT GACCAGCGTC 
ATGCGCCTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGTGGCCAC 
TTCGAGCAGG CCATCAAGGA AGGCGAAGAC ATGATCGCGG AGGAGCACTT CGGGTCAGAG 
AAGATCCGTG AGAGGATCAT TTACATCCGG GAGCAGTGGG CCAACCTAGA GCAGCTCTCA 
GCCATTCGGA AGAAGCGCCT GGAGGAGGCC TCCCTGCTGC ACCAGTTCCA GGCAGATGCT 
GACGACATTG ATGCCTGGAT GCTGGACATC CTCAAGATTG TCTCCAGCAG CGATGTGGGC 
CACGATGAAT ATTCCACACA GTCTCTGGTC AAGAAACACA AGGACGTGGC GGAAGAGATT 
GCCAATTATA GGCCCACCCT TGACACGCTG CACGAGCAAG CCAGCGCCCT CCCCCAGGAG 
CATGCCGAGT CTCCAGATGT GAGGGGCAGG CTGTCGGGCA TCGAGGAGCG ATATAAGGAG 
GTGGCAGAGC TGACGCGGCT GCGGAAGCAG GCACTCCAGG ACACCCTGGC CCTGTACAAG 
ATGTTCAGCG AGGCCGACGC CTGTGAGCTC TGGATCGACG AAAAGGAGCA GTGGCTCAAT 
AACATGCAGA TCCCAGAGAA GCTGGAGGAT CTGGAAGTCA TCCAGCACAG ATTTGAGAGC 
CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTCGCAG TGGTGAACCA GATTGCACGC 
CAGCTGATGC ACAGTGGCAA CCCAAGTGAG AAGGAAATCA AAGCCCAGCA GGACAAACTC 
AACACGAGGT GGAGCCAGTT CAGAGAACTG GTTGACAGGA AGAAGGATGC CCTCCTGTCT 
GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAACGAAA CCAAATCCTG GATTCGGGAA 
AAGACCAAGG TCATCGAGTC CACCCAAGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC 
CTGCAGCGCA AGCTGACCGG CATGGAGCGG GACTTGGTGG CCATTGAGGC AAAGCTGAGT 
GACCTGCAGA AGGAGGCGGA GAAGCTGGAG TCCGAGCACC CCGACCAGGC CCAGGCCATC 
CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACCAC CCTGAAAAAC 
CGAGAAGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTACGGGA CTTGGACGAC 
TTCCAGTCCT GGCTCTCTAG GACCCAGACA GCAATCGCCT CGGAGGACAT GCCAAACACC 
CTGACTGAGG CCGAGAAGCT GCTTACACAG CATGAGAACA TCAAGAACGA GATTGACAAC 
TATGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGGCAGACC 
GATGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TAGACACTGG ATGGAACGAG 
CTGCACAAGA TGTGGGAGAA CAGACAAAAT CTCCTTTCCC AGTCACATGC CTACCAGCAG 
TTCCTCAGAG ACACGAAGCA AGCGGAAGCC TTTCTTAACA ACCAGGAGTA TGTTCTGGCT 
CACACTGAAA TGCCTACCAC CTTGGAAGGA GCTGAGGCAG CAATTAAAAA GCAAGAGGAC 
TTCATGACCA CCATGGACGC CAATGAGGAG AAGATCAACG CTGTGGTGGA GACTGGCCGG 
AGGCTGGTGA GCGATGGGAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCTATT 
GACGACAGAC ATAGGAAGAA TCGTGAGACA GCCAGTGAAC TTTTGATGAG GTTGAAGGAC 
AACAGAGATC TACAGAAATT CCTGCAAGAT TGTCAAGAGC TGTCTCTCTG GATCAATGAG 
AAGATGCTCA CGGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG 
TTGAAGCATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATC 
GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCTGAGA CGGAACCTGT GGTGAAGGAG 
AAACTCACTG GTTTACATGA AATGTGGGAA GTCCTTGAAT CCACTACCCA GACCAAGGCC 
CAGCGGCTCT TTGATGCAAA CAAGGCCGAA CTTTTCACCC AGAGCTGTGC AGATCTAGAC 
AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTACGGCAA AGACCTGACC 
AGTGTCAATA TCCTGCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTGCGGAAG 
AAGGAGATCG AAGAGCTCCA AAGCCAGGCC CAGGCCCTGA GTCAGGAAGG GAAGAGCACC 
GACGAGGTGG ACAGCAAGCG CCTCACCGTG CAGACCAAGT TCATGGAGTT GCTGGAGCCC 
CTGAACGAGA GGAAGCATAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT CAACAGGGAT 
GTGGAGGATG AGATCTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACGGATCAT 
GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAAGAA 
ATCCAGGGAC ACCAGCCTCG CATTGATGAC ATCTTTGAGA GGAGCCAAAA CATCGTCACC 
GACAGCAGCA GCCTCAACGC TGAGGCCATC AGACAGAGGC TTGCCGACCT GAAGCAGCTG 
TGGGGTCTCC TCATTGAGGA GACGGAGAAA CGCCACAGGC GGCTGGAGGA GGCACACCGG 
GCCCAGCAGT ACTACTTTGA CGCTGCCGAG GCCGAAGCCT GGATGAGCGA GCAGGAGCTG 
TACATGATGT CAGAGGAGAA GGCCAAGGAT GAGCAGAGTG CTGTCTCCAT GTTGAAGAAG 
CACCAGATCT TAGAACAAGC TGTGGAGGAC TATGCAGAGA CTGTGCATCA GCTCTCCAAG 
ACCAGCCGGG CCCTGGTGGC CGACAGCCAT CCTGAAAGTG AGCGCATTAG CATGCGGCAG 
TCCAAAGTGG ATAAACTGTA CGCTGGTCTG AAAGACCTTG CTGAAGAGAG AAGAGGCAAG 
CTGGATGAGA GACACAGATT ATTCCAGCTC AACCGGGAGG TAGACGACCT GGAGCAGTGG 
ATCGCTGAGA GGGAGGTGGT CGCAGGGTCC CATGAACTGG GACAGGACTA TGAGCATGTC 
ACGATGTTAC AAGAACGATT CCGGGAATTT GCCCGAGACA CCGGGAACAT TGGACAGGAG 
CGTGTGGACA CGGTCAATCA CATGGCAGAT GAGCTCATCA ACTCTGGCCA TTCAGATGCC 
GCCACCATTG CTGAATGGAA GGACGGCCTC AATGAAGCCT GGGCCGACCT CCTGGAGCTC 
ATTGACACAA GAACACAGAT TCTTGCCGCT TCCTATGAAC TGCACAAGTT TTACCACGAT 
GCCAAGGAGA TCTTTGGGCG TATACAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGG 
AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCACA CTACATTTGA GCATGACATC 
CAGGCTCTGG GCACACAGGT GAGGCAGCTG CAGGAGGATG CAGCCCGCCT CCAGGCAGCC 
TATGCGGGTG ACAAGGCCGA CGACATCCAG AAGCGCGAGA ACGAGGTCCT GGAAGCCTGG 
AAGTCCCTGT TGGACGCCTG CGAGAGCCGC AGGGTGCGGC TGGTGGACAC GGGGGACAAG 
TTCCGCTTCT TCAGCATGGT GCGTGACCTC ATGCTCTGGA TGGAGGATGT CATCCGGCAG 
ATCGAGGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTCTTAAT GAATAATCAT 
CAAGGCATCA AAGCTGAAAT TGATGCACGT AATGACAGTT TCACAACCTG CATTGAACTT 
GGGAAATCCC TGTTGGCAAG AAAACACTAT GCATCTGAGG AGATCAAGGA AAAATTACTG 
CAGTTGACGG AAAAGAGGAA AGAAATGATC GACAAGTGGG AAGACCGATG GGAATGGTTA 
AGACTGATTC TGGAGGTCCA TCAGTTCTCA AGAGACGCCA GTGTGGCCGA GGCCTGGCTG 
CTTGGCCAGG AGCCGTACCT GTCCAGCCGA GAAATAGGCC AGAGTGTGGA CGAGGTGGAG 
AAGCTCATCA AGCGCCACGA GGCATTTGAA AAGTCTGCAG CGACCTGGGA TGAGAGGTTC 
TCTGCCCTGG AAAGGCTGAC TACATTGGAG TTACTGGAAG TGCGCAGACA GCAAGAGGAA 
GAGGAGAGGA AGAGGCGGCC GCCTTCTCCC GAGCCGAGCA CGAAGGTTTC AGAGGAAGCT 
GAGTCCCAGC AGCAGTGGGA TACTGCAAAA GGAGAACAAG TTTCCCAAAA CGGTTTGCCG 
GCTGAACAGG GATCTCCACG GATGGCAGAA ACGGTGGACA CAAGCGAAAT GGTCAACGGC 
GCTACAGAAC AAAGAACGAG CTCTAAAGAG TCCAGCCCCA TCCCCTCCCC AACCTCTGAT 
CGCAAAGCCA AGACTGCCCT CCCAACCCAG AGTGCGGCCA CCTTACCAGC CAGAACCCAG 
GAGACGCCTT CGGCCCAGAT GGAAGGCTTC CTCAATCGGA AACACGAGTG GGAGGCCCAC 
AATAAGAAAG CCTCAAGCAG GTCCTGGCAC AATGTTTATT GTGTCATAAA TAACCAAGAA 
ATGGGTTTCT ACAAAGATGC AAAGACTGCT GCTTCTGGAA TTCCCTACCA CAGCGAGGTC 
CCTGTGAGTT TGAAAGAAGC TGTCTGCGAA GTGGCCCTTG ATTACAAAAA GAAGAAACAC 
GTATTCAAGC TAAGACTAAA TGATGGCAAT GAGTACCTCT TCCAAGCCAA AGACGATGAG 
GAAATGAACA CATGGATCCA GGCCATCTCT TCGGCCATCT CCTCTGATAA ACATGAGGTG 
TCTGCCAGCA CCCAGAGCAC GCCAGCATCC AGCCGGGCGC AGACCCTCCC CACCAGCGTC 
GTCACCATCA CCAGCGAGTC CAGTCCCGGC AAGCGGGAAA AGGACAAAGA GAAAGACAAA 
GAGAAGCGGT TCAGCCTTTT TGGCAAAAAG AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007968821.1
CDS	448..7503
Translation	MELQRTSSISGPLSPAYTGQVPYNYNQLEGRFKQLQDEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLVLSQDYGKHLLGVEDLLQKHTLVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERIIYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSSDVGHDEYSTQSLVKKHKDVAEEIANYRPTLDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGNPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNRETASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEPVVKEKLTGLHEMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLNAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHMADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACESRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKVSEEAESQQQWDTAKGEQVSQNGLPAEQGSPRMAETVDTSEMVNGATEQRTSSKESSPIPSPTSDRKAKTALPTQSAATLPARTQETPSAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKTAASGIPYHSEVPVSLKEAVCEVALDYKKKKHVFKLRLNDGNEYLFQAKDDEEMNTWIQAISSAISSDKHEVSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSLFGKKK

Target ORF information:

RefSeq Version	XM_007970630.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus spectrin, beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007970630.1

ORF Insert Sequence:

ATGGAATTGC AGAGGACGTC TAGCATCTCC GGGCCACTGT CGCCGGCGTA CACGGGGCAG 
GTGCCTTACA ACTACAACCA GCTGGAAGGC AGATTCAAGC AGCTGCAAGA TGAGCGTGAA 
GCCGTACAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCCCG AGTGTCCTGC 
CGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAG 
GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACCAAAGGAC GAATGCGCAT CCACTGCCTA 
GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG 
GGATCCCATG ACATCGTGGA TGGAAACCAC CGGCTGACCC TTGGCCTCAT CTGGACCATC 
ATCCTGCGCT TCCAGATCCA GGATATCAGT GTGGAAACTG AAGACAACAA AGAGAAGAAA 
TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAATGTC 
AACATTCACA ATTTCACCAC TAGCTGGAGG GACGGCATGG CCTTCAATGC ACTGATACAC 
AAACACCGGC CTGACCTGAT AGATTTTGAC AAACTAAAGA AATCCAATGC ACACTACAAC 
CTGCAGAATG CATTTAATCT GGCAGAGCAG CACCTCGGCC TCACTAAACT GTTGGACCCC 
GAAGACATCA GCGTGGACCA CCCTGATGAG AAGTCCATAA TCACTTACGT GGTGACTTAT 
TACCACTACT TCTCTAAGAT GAAGGCTTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG 
CTTGATAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAATCACT TGCCTCTGAC 
CTTCTGGAAT GGATTGAACA AACCATCATC ATTCTGAACA ATCGCAAATT TGCCAATTCA 
CTGGTCGGGG TTCAACAGCA GCTTCAGGCA TTCAACACTT ACCGCACTGT GGAGAAACCA 
CCCAAATTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTCT TCACCATTCA GAGCAAGATG 
AGGGCCAACA ACCAGAAGGT CTACATGCCC CGGGAGGGGA AGCTCATCTC TGACATCAAC 
AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CACGAAAGAG AACTGGCTTT GCGGAATGAG 
CTCATAAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGATCGCAA GGCAGCTATG 
AGGGAGACTT GGCTGAGTGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGGTTTGAC 
CTTCCCGCAG TTGAGGCCGC CACAAAAAAG CACGAGGCCA TTGAGACAGA CATTGCCGCA 
TACGAGGAGC GTGTGCAGGC TGTGGTAGCC GTGGCCAGGG AGCTCGAGGC TGAGAATTAC 
CACGACATCA AGCGCATCAC AGCAAGGAAG GACAATGTCA TCCGACTCTG GGAATACCTA 
CTGGAACTGC TCAGGGCCCG GAGACAGCGG CTCGAGATGA ACCTGGGGCT GCAGAAGATA 
TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGACGAAA TGAAGGTGCT AGTATTGTCT 
CAAGACTATG GCAAACACTT ACTTGGTGTG GAAGACCTGT TACAGAAGCA CACCCTGGTT 
GAAGCAGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCCTC TGCCCAGAAG 
TTCGCAACAG ACGGGGAAGG TTACAAGCCC TGCGACCCCC AGGTGATCCG AGACCGCGTG 
GCCCACATGG AGTTCTGTTA TCAAGAGCTT TGCCAGCTGG CGGCTGAGCG CAGGGCCCGT 
CTGGAAGAGT CCCGCCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAAGGCTGG 
ATACGGGAGA AGGAGAAGAT CCTGTCCTCA GACGATTATG GGAAAGACCT GACCAGCGTC 
ATGCGCCTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGTGGCCAC 
TTCGAGCAGG CCATCAAGGA AGGCGAAGAC ATGATCGCGG AGGAGCACTT CGGGTCAGAG 
AAGATCCGTG AGAGGATCAT TTACATCCGG GAGCAGTGGG CCAACCTAGA GCAGCTCTCA 
GCCATTCGGA AGAAGCGCCT GGAGGAGGCC TCCCTGCTGC ACCAGTTCCA GGCAGATGCT 
GACGACATTG ATGCCTGGAT GCTGGACATC CTCAAGATTG TCTCCAGCAG CGATGTGGGC 
CACGATGAAT ATTCCACACA GTCTCTGGTC AAGAAACACA AGGACGTGGC GGAAGAGATT 
GCCAATTATA GGCCCACCCT TGACACGCTG CACGAGCAAG CCAGCGCCCT CCCCCAGGAG 
CATGCCGAGT CTCCAGATGT GAGGGGCAGG CTGTCGGGCA TCGAGGAGCG ATATAAGGAG 
GTGGCAGAGC TGACGCGGCT GCGGAAGCAG GCACTCCAGG ACACCCTGGC CCTGTACAAG 
ATGTTCAGCG AGGCCGACGC CTGTGAGCTC TGGATCGACG AAAAGGAGCA GTGGCTCAAT 
AACATGCAGA TCCCAGAGAA GCTGGAGGAT CTGGAAGTCA TCCAGCACAG ATTTGAGAGC 
CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTCGCAG TGGTGAACCA GATTGCACGC 
CAGCTGATGC ACAGTGGCAA CCCAAGTGAG AAGGAAATCA AAGCCCAGCA GGACAAACTC 
AACACGAGGT GGAGCCAGTT CAGAGAACTG GTTGACAGGA AGAAGGATGC CCTCCTGTCT 
GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAACGAAA CCAAATCCTG GATTCGGGAA 
AAGACCAAGG TCATCGAGTC CACCCAAGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC 
CTGCAGCGCA AGCTGACCGG CATGGAGCGG GACTTGGTGG CCATTGAGGC AAAGCTGAGT 
GACCTGCAGA AGGAGGCGGA GAAGCTGGAG TCCGAGCACC CCGACCAGGC CCAGGCCATC 
CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACCAC CCTGAAAAAC 
CGAGAAGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTACGGGA CTTGGACGAC 
TTCCAGTCCT GGCTCTCTAG GACCCAGACA GCAATCGCCT CGGAGGACAT GCCAAACACC 
CTGACTGAGG CCGAGAAGCT GCTTACACAG CATGAGAACA TCAAGAACGA GATTGACAAC 
TATGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGGCAGACC 
GATGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TAGACACTGG ATGGAACGAG 
CTGCACAAGA TGTGGGAGAA CAGACAAAAT CTCCTTTCCC AGTCACATGC CTACCAGCAG 
TTCCTCAGAG ACACGAAGCA AGCGGAAGCC TTTCTTAACA ACCAGGAGTA TGTTCTGGCT 
CACACTGAAA TGCCTACCAC CTTGGAAGGA GCTGAGGCAG CAATTAAAAA GCAAGAGGAC 
TTCATGACCA CCATGGACGC CAATGAGGAG AAGATCAACG CTGTGGTGGA GACTGGCCGG 
AGGCTGGTGA GCGATGGGAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCTATT 
GACGACAGAC ATAGGAAGAA TCGTGAGACA GCCAGTGAAC TTTTGATGAG GTTGAAGGAC 
AACAGAGATC TACAGAAATT CCTGCAAGAT TGTCAAGAGC TGTCTCTCTG GATCAATGAG 
AAGATGCTCA CGGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG 
TTGAAGCATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATC 
GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCTGAGA CGGAACCTGT GGTGAAGGAG 
AAACTCACTG GTTTACATGA AATGTGGGAA GTCCTTGAAT CCACTACCCA GACCAAGGCC 
CAGCGGCTCT TTGATGCAAA CAAGGCCGAA CTTTTCACCC AGAGCTGTGC AGATCTAGAC 
AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTACGGCAA AGACCTGACC 
AGTGTCAATA TCCTGCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTGCGGAAG 
AAGGAGATCG AAGAGCTCCA AAGCCAGGCC CAGGCCCTGA GTCAGGAAGG GAAGAGCACC 
GACGAGGTGG ACAGCAAGCG CCTCACCGTG CAGACCAAGT TCATGGAGTT GCTGGAGCCC 
CTGAACGAGA GGAAGCATAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT CAACAGGGAT 
GTGGAGGATG AGATCTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACGGATCAT 
GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAAGAA 
ATCCAGGGAC ACCAGCCTCG CATTGATGAC ATCTTTGAGA GGAGCCAAAA CATCGTCACC 
GACAGCAGCA GCCTCAACGC TGAGGCCATC AGACAGAGGC TTGCCGACCT GAAGCAGCTG 
TGGGGTCTCC TCATTGAGGA GACGGAGAAA CGCCACAGGC GGCTGGAGGA GGCACACCGG 
GCCCAGCAGT ACTACTTTGA CGCTGCCGAG GCCGAAGCCT GGATGAGCGA GCAGGAGCTG 
TACATGATGT CAGAGGAGAA GGCCAAGGAT GAGCAGAGTG CTGTCTCCAT GTTGAAGAAG 
CACCAGATCT TAGAACAAGC TGTGGAGGAC TATGCAGAGA CTGTGCATCA GCTCTCCAAG 
ACCAGCCGGG CCCTGGTGGC CGACAGCCAT CCTGAAAGTG AGCGCATTAG CATGCGGCAG 
TCCAAAGTGG ATAAACTGTA CGCTGGTCTG AAAGACCTTG CTGAAGAGAG AAGAGGCAAG 
CTGGATGAGA GACACAGATT ATTCCAGCTC AACCGGGAGG TAGACGACCT GGAGCAGTGG 
ATCGCTGAGA GGGAGGTGGT CGCAGGGTCC CATGAACTGG GACAGGACTA TGAGCATGTC 
ACGATGTTAC AAGAACGATT CCGGGAATTT GCCCGAGACA CCGGGAACAT TGGACAGGAG 
CGTGTGGACA CGGTCAATCA CATGGCAGAT GAGCTCATCA ACTCTGGCCA TTCAGATGCC 
GCCACCATTG CTGAATGGAA GGACGGCCTC AATGAAGCCT GGGCCGACCT CCTGGAGCTC 
ATTGACACAA GAACACAGAT TCTTGCCGCT TCCTATGAAC TGCACAAGTT TTACCACGAT 
GCCAAGGAGA TCTTTGGGCG TATACAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGG 
AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCACA CTACATTTGA GCATGACATC 
CAGGCTCTGG GCACACAGGT GAGGCAGCTG CAGGAGGATG CAGCCCGCCT CCAGGCAGCC 
TATGCGGGTG ACAAGGCCGA CGACATCCAG AAGCGCGAGA ACGAGGTCCT GGAAGCCTGG 
AAGTCCCTGT TGGACGCCTG CGAGAGCCGC AGGGTGCGGC TGGTGGACAC GGGGGACAAG 
TTCCGCTTCT TCAGCATGGT GCGTGACCTC ATGCTCTGGA TGGAGGATGT CATCCGGCAG 
ATCGAGGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTCTTAAT GAATAATCAT 
CAAGGCATCA AAGCTGAAAT TGATGCACGT AATGACAGTT TCACAACCTG CATTGAACTT 
GGGAAATCCC TGTTGGCAAG AAAACACTAT GCATCTGAGG AGATCAAGGA AAAATTACTG 
CAGTTGACGG AAAAGAGGAA AGAAATGATC GACAAGTGGG AAGACCGATG GGAATGGTTA 
AGACTGATTC TGGAGGTCCA TCAGTTCTCA AGAGACGCCA GTGTGGCCGA GGCCTGGCTG 
CTTGGCCAGG AGCCGTACCT GTCCAGCCGA GAAATAGGCC AGAGTGTGGA CGAGGTGGAG 
AAGCTCATCA AGCGCCACGA GGCATTTGAA AAGTCTGCAG CGACCTGGGA TGAGAGGTTC 
TCTGCCCTGG AAAGGCTGAC TACATTGGAG TTACTGGAAG TGCGCAGACA GCAAGAGGAA 
GAGGAGAGGA AGAGGCGGCC GCCTTCTCCC GAGCCGAGCA CGAAGGTTTC AGAGGAAGCT 
GAGTCCCAGC AGCAGTGGGA TACTGCAAAA GGAGAACAAG TTTCCCAAAA CGGTTTGCCG 
GCTGAACAGG GATCTCCACG GATGGCAGAA ACGGTGGACA CAAGCGAAAT GGTCAACGGC 
GCTACAGAAC AAAGAACGAG CTCTAAAGAG TCCAGCCCCA TCCCCTCCCC AACCTCTGAT 
CGCAAAGCCA AGACTGCCCT CCCAACCCAG AGTGCGGCCA CCTTACCAGC CAGAACCCAG 
GAGACGCCTT CGGCCCAGAT GGAAGGCTTC CTCAATCGGA AACACGAGTG GGAGGCCCAC 
AATAAGAAAG CCTCAAGCAG GTCCTGGCAC AATGTTTATT GTGTCATAAA TAACCAAGAA 
ATGGGTTTCT ACAAAGATGC AAAGACTGCT GCTTCTGGAA TTCCCTACCA CAGCGAGGTC 
CCTGTGAGTT TGAAAGAAGC TGTCTGCGAA GTGGCCCTTG ATTACAAAAA GAAGAAACAC 
GTATTCAAGC TAAGACTAAA TGATGGCAAT GAGTACCTCT TCCAAGCCAA AGACGATGAG 
GAAATGAACA CATGGATCCA GGCCATCTCT TCGGCCATCT CCTCTGATAA ACATGAGGTG 
TCTGCCAGCA CCCAGAGCAC GCCAGCATCC AGCCGGGCGC AGACCCTCCC CACCAGCGTC 
GTCACCATCA CCAGCGAGTC CAGTCCCGGC AAGCGGGAAA AGGACAAAGA GAAAGACAAA 
GAGAAGCGGT TCAGCCTTTT TGGCAAAAAG AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTBN1 ( XM_007970631.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007968822.1 Chlorocebus sabaeus spectrin, beta, non-erythrocytic 1 (SPTBN1), transcript variant X4, mRNA.

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CloneID	OCh102615
Clone ID Related Accession (Same CDS sequence)	XM_007970631.1
Accession Version	XM_007970631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	spectrin beta chain, non-erythrocytic 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735260.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTGC AGAGGACGTC TAGCATCTCC GGGCCACTGT CGCCGGCGTA CACGGGGCAG 
GTGCCTTACA ACTACAACCA GCTGGAAGGC AGATTCAAGC AGCTGCAAGA TGAGCGTGAA 
GCCGTACAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCCCG AGTGTCCTGC 
CGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAG 
GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACCAAAGGAC GAATGCGCAT CCACTGCCTA 
GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG 
GGATCCCATG ACATCGTGGA TGGAAACCAC CGGCTGACCC TTGGCCTCAT CTGGACCATC 
ATCCTGCGCT TCCAGATCCA GGATATCAGT GTGGAAACTG AAGACAACAA AGAGAAGAAA 
TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAATGTC 
AACATTCACA ATTTCACCAC TAGCTGGAGG GACGGCATGG CCTTCAATGC ACTGATACAC 
AAACACCGGC CTGACCTGAT AGATTTTGAC AAACTAAAGA AATCCAATGC ACACTACAAC 
CTGCAGAATG CATTTAATCT GGCAGAGCAG CACCTCGGCC TCACTAAACT GTTGGACCCC 
GAAGACATCA GCGTGGACCA CCCTGATGAG AAGTCCATAA TCACTTACGT GGTGACTTAT 
TACCACTACT TCTCTAAGAT GAAGGCTTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG 
CTTGATAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAATCACT TGCCTCTGAC 
CTTCTGGAAT GGATTGAACA AACCATCATC ATTCTGAACA ATCGCAAATT TGCCAATTCA 
CTGGTCGGGG TTCAACAGCA GCTTCAGGCA TTCAACACTT ACCGCACTGT GGAGAAACCA 
CCCAAATTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTCT TCACCATTCA GAGCAAGATG 
AGGGCCAACA ACCAGAAGGT CTACATGCCC CGGGAGGGGA AGCTCATCTC TGACATCAAC 
AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CACGAAAGAG AACTGGCTTT GCGGAATGAG 
CTCATAAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGATCGCAA GGCAGCTATG 
AGGGAGACTT GGCTGAGTGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGGTTTGAC 
CTTCCCGCAG TTGAGGCCGC CACAAAAAAG CACGAGGCCA TTGAGACAGA CATTGCCGCA 
TACGAGGAGC GTGTGCAGGC TGTGGTAGCC GTGGCCAGGG AGCTCGAGGC TGAGAATTAC 
CACGACATCA AGCGCATCAC AGCAAGGAAG GACAATGTCA TCCGACTCTG GGAATACCTA 
CTGGAACTGC TCAGGGCCCG GAGACAGCGG CTCGAGATGA ACCTGGGGCT GCAGAAGATA 
TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGACGAAA TGAAGGTGCT AGTATTGTCT 
CAAGACTATG GCAAACACTT ACTTGGTGTG GAAGACCTGT TACAGAAGCA CACCCTGGTT 
GAAGCAGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCCTC TGCCCAGAAG 
TTCGCAACAG ACGGGGAAGG TTACAAGCCC TGCGACCCCC AGGTGATCCG AGACCGCGTG 
GCCCACATGG AGTTCTGTTA TCAAGAGCTT TGCCAGCTGG CGGCTGAGCG CAGGGCCCGT 
CTGGAAGAGT CCCGCCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAAGGCTGG 
ATACGGGAGA AGGAGAAGAT CCTGTCCTCA GACGATTATG GGAAAGACCT GACCAGCGTC 
ATGCGCCTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGTGGCCAC 
TTCGAGCAGG CCATCAAGGA AGGCGAAGAC ATGATCGCGG AGGAGCACTT CGGGTCAGAG 
AAGATCCGTG AGAGGATCAT TTACATCCGG GAGCAGTGGG CCAACCTAGA GCAGCTCTCA 
GCCATTCGGA AGAAGCGCCT GGAGGAGGCC TCCCTGCTGC ACCAGTTCCA GGCAGATGCT 
GACGACATTG ATGCCTGGAT GCTGGACATC CTCAAGATTG TCTCCAGCAG CGATGTGGGC 
CACGATGAAT ATTCCACACA GTCTCTGGTC AAGAAACACA AGGACGTGGC GGAAGAGATT 
GCCAATTATA GGCCCACCCT TGACACGCTG CACGAGCAAG CCAGCGCCCT CCCCCAGGAG 
CATGCCGAGT CTCCAGATGT GAGGGGCAGG CTGTCGGGCA TCGAGGAGCG ATATAAGGAG 
GTGGCAGAGC TGACGCGGCT GCGGAAGCAG GCACTCCAGG ACACCCTGGC CCTGTACAAG 
ATGTTCAGCG AGGCCGACGC CTGTGAGCTC TGGATCGACG AAAAGGAGCA GTGGCTCAAT 
AACATGCAGA TCCCAGAGAA GCTGGAGGAT CTGGAAGTCA TCCAGCACAG ATTTGAGAGC 
CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTCGCAG TGGTGAACCA GATTGCACGC 
CAGCTGATGC ACAGTGGCAA CCCAAGTGAG AAGGAAATCA AAGCCCAGCA GGACAAACTC 
AACACGAGGT GGAGCCAGTT CAGAGAACTG GTTGACAGGA AGAAGGATGC CCTCCTGTCT 
GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAACGAAA CCAAATCCTG GATTCGGGAA 
AAGACCAAGG TCATCGAGTC CACCCAAGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC 
CTGCAGCGCA AGCTGACCGG CATGGAGCGG GACTTGGTGG CCATTGAGGC AAAGCTGAGT 
GACCTGCAGA AGGAGGCGGA GAAGCTGGAG TCCGAGCACC CCGACCAGGC CCAGGCCATC 
CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACCAC CCTGAAAAAC 
CGAGAAGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTACGGGA CTTGGACGAC 
TTCCAGTCCT GGCTCTCTAG GACCCAGACA GCAATCGCCT CGGAGGACAT GCCAAACACC 
CTGACTGAGG CCGAGAAGCT GCTTACACAG CATGAGAACA TCAAGAACGA GATTGACAAC 
TATGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGGCAGACC 
GATGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TAGACACTGG ATGGAACGAG 
CTGCACAAGA TGTGGGAGAA CAGACAAAAT CTCCTTTCCC AGTCACATGC CTACCAGCAG 
TTCCTCAGAG ACACGAAGCA AGCGGAAGCC TTTCTTAACA ACCAGGAGTA TGTTCTGGCT 
CACACTGAAA TGCCTACCAC CTTGGAAGGA GCTGAGGCAG CAATTAAAAA GCAAGAGGAC 
TTCATGACCA CCATGGACGC CAATGAGGAG AAGATCAACG CTGTGGTGGA GACTGGCCGG 
AGGCTGGTGA GCGATGGGAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCTATT 
GACGACAGAC ATAGGAAGAA TCGTGAGACA GCCAGTGAAC TTTTGATGAG GTTGAAGGAC 
AACAGAGATC TACAGAAATT CCTGCAAGAT TGTCAAGAGC TGTCTCTCTG GATCAATGAG 
AAGATGCTCA CGGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG 
TTGAAGCATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATC 
GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCTGAGA CGGAACCTGT GGTGAAGGAG 
AAACTCACTG GTTTACATGA AATGTGGGAA GTCCTTGAAT CCACTACCCA GACCAAGGCC 
CAGCGGCTCT TTGATGCAAA CAAGGCCGAA CTTTTCACCC AGAGCTGTGC AGATCTAGAC 
AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTACGGCAA AGACCTGACC 
AGTGTCAATA TCCTGCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTGCGGAAG 
AAGGAGATCG AAGAGCTCCA AAGCCAGGCC CAGGCCCTGA GTCAGGAAGG GAAGAGCACC 
GACGAGGTGG ACAGCAAGCG CCTCACCGTG CAGACCAAGT TCATGGAGTT GCTGGAGCCC 
CTGAACGAGA GGAAGCATAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT CAACAGGGAT 
GTGGAGGATG AGATCTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACGGATCAT 
GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAAGAA 
ATCCAGGGAC ACCAGCCTCG CATTGATGAC ATCTTTGAGA GGAGCCAAAA CATCGTCACC 
GACAGCAGCA GCCTCAACGC TGAGGCCATC AGACAGAGGC TTGCCGACCT GAAGCAGCTG 
TGGGGTCTCC TCATTGAGGA GACGGAGAAA CGCCACAGGC GGCTGGAGGA GGCACACCGG 
GCCCAGCAGT ACTACTTTGA CGCTGCCGAG GCCGAAGCCT GGATGAGCGA GCAGGAGCTG 
TACATGATGT CAGAGGAGAA GGCCAAGGAT GAGCAGAGTG CTGTCTCCAT GTTGAAGAAG 
CACCAGATCT TAGAACAAGC TGTGGAGGAC TATGCAGAGA CTGTGCATCA GCTCTCCAAG 
ACCAGCCGGG CCCTGGTGGC CGACAGCCAT CCTGAAAGTG AGCGCATTAG CATGCGGCAG 
TCCAAAGTGG ATAAACTGTA CGCTGGTCTG AAAGACCTTG CTGAAGAGAG AAGAGGCAAG 
CTGGATGAGA GACACAGATT ATTCCAGCTC AACCGGGAGG TAGACGACCT GGAGCAGTGG 
ATCGCTGAGA GGGAGGTGGT CGCAGGGTCC CATGAACTGG GACAGGACTA TGAGCATGTC 
ACGATGTTAC AAGAACGATT CCGGGAATTT GCCCGAGACA CCGGGAACAT TGGACAGGAG 
CGTGTGGACA CGGTCAATCA CATGGCAGAT GAGCTCATCA ACTCTGGCCA TTCAGATGCC 
GCCACCATTG CTGAATGGAA GGACGGCCTC AATGAAGCCT GGGCCGACCT CCTGGAGCTC 
ATTGACACAA GAACACAGAT TCTTGCCGCT TCCTATGAAC TGCACAAGTT TTACCACGAT 
GCCAAGGAGA TCTTTGGGCG TATACAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGG 
AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCACA CTACATTTGA GCATGACATC 
CAGGCTCTGG GCACACAGGT GAGGCAGCTG CAGGAGGATG CAGCCCGCCT CCAGGCAGCC 
TATGCGGGTG ACAAGGCCGA CGACATCCAG AAGCGCGAGA ACGAGGTCCT GGAAGCCTGG 
AAGTCCCTGT TGGACGCCTG CGAGAGCCGC AGGGTGCGGC TGGTGGACAC GGGGGACAAG 
TTCCGCTTCT TCAGCATGGT GCGTGACCTC ATGCTCTGGA TGGAGGATGT CATCCGGCAG 
ATCGAGGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTCTTAAT GAATAATCAT 
CAAGGCATCA AAGCTGAAAT TGATGCACGT AATGACAGTT TCACAACCTG CATTGAACTT 
GGGAAATCCC TGTTGGCAAG AAAACACTAT GCATCTGAGG AGATCAAGGA AAAATTACTG 
CAGTTGACGG AAAAGAGGAA AGAAATGATC GACAAGTGGG AAGACCGATG GGAATGGTTA 
AGACTGATTC TGGAGGTCCA TCAGTTCTCA AGAGACGCCA GTGTGGCCGA GGCCTGGCTG 
CTTGGCCAGG AGCCGTACCT GTCCAGCCGA GAAATAGGCC AGAGTGTGGA CGAGGTGGAG 
AAGCTCATCA AGCGCCACGA GGCATTTGAA AAGTCTGCAG CGACCTGGGA TGAGAGGTTC 
TCTGCCCTGG AAAGGCTGAC TACATTGGAG TTACTGGAAG TGCGCAGACA GCAAGAGGAA 
GAGGAGAGGA AGAGGCGGCC GCCTTCTCCC GAGCCGAGCA CGAAGGTTTC AGAGGAAGCT 
GAGTCCCAGC AGCAGTGGGA TACTGCAAAA GGAGAACAAG TTTCCCAAAA CGGTTTGCCG 
GCTGAACAGG GATCTCCACG GGTTAGTTAC CGCTCTCAAA CCTACCAAAA CTACAAAAAC 
TTTAATAGCA GACGGACAGC CAGTGACCAG CCATGGTCTG GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007968822.1
CDS	446..6913
Translation	MELQRTSSISGPLSPAYTGQVPYNYNQLEGRFKQLQDEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLVLSQDYGKHLLGVEDLLQKHTLVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERIIYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSSDVGHDEYSTQSLVKKHKDVAEEIANYRPTLDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGNPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNRETASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEPVVKEKLTGLHEMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLNAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHMADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACESRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKVSEEAESQQQWDTAKGEQVSQNGLPAEQGSPRVSYRSQTYQNYKNFNSRRTASDQPWSGL

Target ORF information:

RefSeq Version	XM_007970631.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus spectrin, beta, non-erythrocytic 1 (SPTBN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007970631.1

ORF Insert Sequence:

ATGGAATTGC AGAGGACGTC TAGCATCTCC GGGCCACTGT CGCCGGCGTA CACGGGGCAG 
GTGCCTTACA ACTACAACCA GCTGGAAGGC AGATTCAAGC AGCTGCAAGA TGAGCGTGAA 
GCCGTACAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCCCG AGTGTCCTGC 
CGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAG 
GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACCAAAGGAC GAATGCGCAT CCACTGCCTA 
GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG 
GGATCCCATG ACATCGTGGA TGGAAACCAC CGGCTGACCC TTGGCCTCAT CTGGACCATC 
ATCCTGCGCT TCCAGATCCA GGATATCAGT GTGGAAACTG AAGACAACAA AGAGAAGAAA 
TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAATGTC 
AACATTCACA ATTTCACCAC TAGCTGGAGG GACGGCATGG CCTTCAATGC ACTGATACAC 
AAACACCGGC CTGACCTGAT AGATTTTGAC AAACTAAAGA AATCCAATGC ACACTACAAC 
CTGCAGAATG CATTTAATCT GGCAGAGCAG CACCTCGGCC TCACTAAACT GTTGGACCCC 
GAAGACATCA GCGTGGACCA CCCTGATGAG AAGTCCATAA TCACTTACGT GGTGACTTAT 
TACCACTACT TCTCTAAGAT GAAGGCTTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG 
CTTGATAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAATCACT TGCCTCTGAC 
CTTCTGGAAT GGATTGAACA AACCATCATC ATTCTGAACA ATCGCAAATT TGCCAATTCA 
CTGGTCGGGG TTCAACAGCA GCTTCAGGCA TTCAACACTT ACCGCACTGT GGAGAAACCA 
CCCAAATTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTCT TCACCATTCA GAGCAAGATG 
AGGGCCAACA ACCAGAAGGT CTACATGCCC CGGGAGGGGA AGCTCATCTC TGACATCAAC 
AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CACGAAAGAG AACTGGCTTT GCGGAATGAG 
CTCATAAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGATCGCAA GGCAGCTATG 
AGGGAGACTT GGCTGAGTGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGGTTTGAC 
CTTCCCGCAG TTGAGGCCGC CACAAAAAAG CACGAGGCCA TTGAGACAGA CATTGCCGCA 
TACGAGGAGC GTGTGCAGGC TGTGGTAGCC GTGGCCAGGG AGCTCGAGGC TGAGAATTAC 
CACGACATCA AGCGCATCAC AGCAAGGAAG GACAATGTCA TCCGACTCTG GGAATACCTA 
CTGGAACTGC TCAGGGCCCG GAGACAGCGG CTCGAGATGA ACCTGGGGCT GCAGAAGATA 
TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGACGAAA TGAAGGTGCT AGTATTGTCT 
CAAGACTATG GCAAACACTT ACTTGGTGTG GAAGACCTGT TACAGAAGCA CACCCTGGTT 
GAAGCAGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCCTC TGCCCAGAAG 
TTCGCAACAG ACGGGGAAGG TTACAAGCCC TGCGACCCCC AGGTGATCCG AGACCGCGTG 
GCCCACATGG AGTTCTGTTA TCAAGAGCTT TGCCAGCTGG CGGCTGAGCG CAGGGCCCGT 
CTGGAAGAGT CCCGCCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAAGGCTGG 
ATACGGGAGA AGGAGAAGAT CCTGTCCTCA GACGATTATG GGAAAGACCT GACCAGCGTC 
ATGCGCCTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGTGGCCAC 
TTCGAGCAGG CCATCAAGGA AGGCGAAGAC ATGATCGCGG AGGAGCACTT CGGGTCAGAG 
AAGATCCGTG AGAGGATCAT TTACATCCGG GAGCAGTGGG CCAACCTAGA GCAGCTCTCA 
GCCATTCGGA AGAAGCGCCT GGAGGAGGCC TCCCTGCTGC ACCAGTTCCA GGCAGATGCT 
GACGACATTG ATGCCTGGAT GCTGGACATC CTCAAGATTG TCTCCAGCAG CGATGTGGGC 
CACGATGAAT ATTCCACACA GTCTCTGGTC AAGAAACACA AGGACGTGGC GGAAGAGATT 
GCCAATTATA GGCCCACCCT TGACACGCTG CACGAGCAAG CCAGCGCCCT CCCCCAGGAG 
CATGCCGAGT CTCCAGATGT GAGGGGCAGG CTGTCGGGCA TCGAGGAGCG ATATAAGGAG 
GTGGCAGAGC TGACGCGGCT GCGGAAGCAG GCACTCCAGG ACACCCTGGC CCTGTACAAG 
ATGTTCAGCG AGGCCGACGC CTGTGAGCTC TGGATCGACG AAAAGGAGCA GTGGCTCAAT 
AACATGCAGA TCCCAGAGAA GCTGGAGGAT CTGGAAGTCA TCCAGCACAG ATTTGAGAGC 
CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTCGCAG TGGTGAACCA GATTGCACGC 
CAGCTGATGC ACAGTGGCAA CCCAAGTGAG AAGGAAATCA AAGCCCAGCA GGACAAACTC 
AACACGAGGT GGAGCCAGTT CAGAGAACTG GTTGACAGGA AGAAGGATGC CCTCCTGTCT 
GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAACGAAA CCAAATCCTG GATTCGGGAA 
AAGACCAAGG TCATCGAGTC CACCCAAGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC 
CTGCAGCGCA AGCTGACCGG CATGGAGCGG GACTTGGTGG CCATTGAGGC AAAGCTGAGT 
GACCTGCAGA AGGAGGCGGA GAAGCTGGAG TCCGAGCACC CCGACCAGGC CCAGGCCATC 
CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACCAC CCTGAAAAAC 
CGAGAAGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTACGGGA CTTGGACGAC 
TTCCAGTCCT GGCTCTCTAG GACCCAGACA GCAATCGCCT CGGAGGACAT GCCAAACACC 
CTGACTGAGG CCGAGAAGCT GCTTACACAG CATGAGAACA TCAAGAACGA GATTGACAAC 
TATGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGGCAGACC 
GATGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TAGACACTGG ATGGAACGAG 
CTGCACAAGA TGTGGGAGAA CAGACAAAAT CTCCTTTCCC AGTCACATGC CTACCAGCAG 
TTCCTCAGAG ACACGAAGCA AGCGGAAGCC TTTCTTAACA ACCAGGAGTA TGTTCTGGCT 
CACACTGAAA TGCCTACCAC CTTGGAAGGA GCTGAGGCAG CAATTAAAAA GCAAGAGGAC 
TTCATGACCA CCATGGACGC CAATGAGGAG AAGATCAACG CTGTGGTGGA GACTGGCCGG 
AGGCTGGTGA GCGATGGGAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCTATT 
GACGACAGAC ATAGGAAGAA TCGTGAGACA GCCAGTGAAC TTTTGATGAG GTTGAAGGAC 
AACAGAGATC TACAGAAATT CCTGCAAGAT TGTCAAGAGC TGTCTCTCTG GATCAATGAG 
AAGATGCTCA CGGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG 
TTGAAGCATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATC 
GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCTGAGA CGGAACCTGT GGTGAAGGAG 
AAACTCACTG GTTTACATGA AATGTGGGAA GTCCTTGAAT CCACTACCCA GACCAAGGCC 
CAGCGGCTCT TTGATGCAAA CAAGGCCGAA CTTTTCACCC AGAGCTGTGC AGATCTAGAC 
AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTACGGCAA AGACCTGACC 
AGTGTCAATA TCCTGCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTGCGGAAG 
AAGGAGATCG AAGAGCTCCA AAGCCAGGCC CAGGCCCTGA GTCAGGAAGG GAAGAGCACC 
GACGAGGTGG ACAGCAAGCG CCTCACCGTG CAGACCAAGT TCATGGAGTT GCTGGAGCCC 
CTGAACGAGA GGAAGCATAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT CAACAGGGAT 
GTGGAGGATG AGATCTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACGGATCAT 
GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAAGAA 
ATCCAGGGAC ACCAGCCTCG CATTGATGAC ATCTTTGAGA GGAGCCAAAA CATCGTCACC 
GACAGCAGCA GCCTCAACGC TGAGGCCATC AGACAGAGGC TTGCCGACCT GAAGCAGCTG 
TGGGGTCTCC TCATTGAGGA GACGGAGAAA CGCCACAGGC GGCTGGAGGA GGCACACCGG 
GCCCAGCAGT ACTACTTTGA CGCTGCCGAG GCCGAAGCCT GGATGAGCGA GCAGGAGCTG 
TACATGATGT CAGAGGAGAA GGCCAAGGAT GAGCAGAGTG CTGTCTCCAT GTTGAAGAAG 
CACCAGATCT TAGAACAAGC TGTGGAGGAC TATGCAGAGA CTGTGCATCA GCTCTCCAAG 
ACCAGCCGGG CCCTGGTGGC CGACAGCCAT CCTGAAAGTG AGCGCATTAG CATGCGGCAG 
TCCAAAGTGG ATAAACTGTA CGCTGGTCTG AAAGACCTTG CTGAAGAGAG AAGAGGCAAG 
CTGGATGAGA GACACAGATT ATTCCAGCTC AACCGGGAGG TAGACGACCT GGAGCAGTGG 
ATCGCTGAGA GGGAGGTGGT CGCAGGGTCC CATGAACTGG GACAGGACTA TGAGCATGTC 
ACGATGTTAC AAGAACGATT CCGGGAATTT GCCCGAGACA CCGGGAACAT TGGACAGGAG 
CGTGTGGACA CGGTCAATCA CATGGCAGAT GAGCTCATCA ACTCTGGCCA TTCAGATGCC 
GCCACCATTG CTGAATGGAA GGACGGCCTC AATGAAGCCT GGGCCGACCT CCTGGAGCTC 
ATTGACACAA GAACACAGAT TCTTGCCGCT TCCTATGAAC TGCACAAGTT TTACCACGAT 
GCCAAGGAGA TCTTTGGGCG TATACAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGG 
AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCACA CTACATTTGA GCATGACATC 
CAGGCTCTGG GCACACAGGT GAGGCAGCTG CAGGAGGATG CAGCCCGCCT CCAGGCAGCC 
TATGCGGGTG ACAAGGCCGA CGACATCCAG AAGCGCGAGA ACGAGGTCCT GGAAGCCTGG 
AAGTCCCTGT TGGACGCCTG CGAGAGCCGC AGGGTGCGGC TGGTGGACAC GGGGGACAAG 
TTCCGCTTCT TCAGCATGGT GCGTGACCTC ATGCTCTGGA TGGAGGATGT CATCCGGCAG 
ATCGAGGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTCTTAAT GAATAATCAT 
CAAGGCATCA AAGCTGAAAT TGATGCACGT AATGACAGTT TCACAACCTG CATTGAACTT 
GGGAAATCCC TGTTGGCAAG AAAACACTAT GCATCTGAGG AGATCAAGGA AAAATTACTG 
CAGTTGACGG AAAAGAGGAA AGAAATGATC GACAAGTGGG AAGACCGATG GGAATGGTTA 
AGACTGATTC TGGAGGTCCA TCAGTTCTCA AGAGACGCCA GTGTGGCCGA GGCCTGGCTG 
CTTGGCCAGG AGCCGTACCT GTCCAGCCGA GAAATAGGCC AGAGTGTGGA CGAGGTGGAG 
AAGCTCATCA AGCGCCACGA GGCATTTGAA AAGTCTGCAG CGACCTGGGA TGAGAGGTTC 
TCTGCCCTGG AAAGGCTGAC TACATTGGAG TTACTGGAAG TGCGCAGACA GCAAGAGGAA 
GAGGAGAGGA AGAGGCGGCC GCCTTCTCCC GAGCCGAGCA CGAAGGTTTC AGAGGAAGCT 
GAGTCCCAGC AGCAGTGGGA TACTGCAAAA GGAGAACAAG TTTCCCAAAA CGGTTTGCCG 
GCTGAACAGG GATCTCCACG GGTTAGTTAC CGCTCTCAAA CCTACCAAAA CTACAAAAAC 
TTTAATAGCA GACGGACAGC CAGTGACCAG CCATGGTCTG GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SPTBN1 cDNA ORF clone, Chlorocebus sabaeus(green monkey)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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