eBlot™ Protein Transfer System

Selection Guide eBlot™ L2 eBlot™ L1 Testimonial Service Plans Resources Trade-In

Catalog Products » Protein Electrophoresis and Blotting » eBlot™ Protein Transfer System

GenScript's eBlot™ technology seamlessly combines the benefits of traditional wet transfer and semi-dry/dry transfer methods, ensuring exceptional transfer efficiency and consistent results for proteins of all sizes.
eBlot™ L2 Fast Wet Transfer System, the second-generation protein transfer instrument is upgraded for transfer of up to 4 mini gels or 2 midi gels in 5-15 minutes.

Fast wet transfer

in 5-15 minutes

High throughput transfer

of 4 mini or 2 midi gels

Full protein size

coverage on PVDF
or NC membranes

Alcohol-free

reagents with DI water dilution

Selection Guide

Features	eBlot™ L2	eBlot™ L1

Midi Gel Compatibility and Flexible Throughput
Alcohol-free Reagents
Smaller Footprint
Gold Standard Wet Transfer Efficiency
Full Protein Size Coverage
Fast Automated Transfer

eBlot™ L2

eBlot™ L2 Video

Introducing eBlot L2, High-throughput Fast Wet Transfer System

Demo Available

Why Choose eBlot™ L2

Conventional wet transfers are cumbersome, require experience and a great deal of time spent on consumable preparation. The transfer times usually vary from 1 hour to overnight. With eBlot™ fast wet transfer system, achieve excellent transfer efficiency without sacrificing speed.

Convenience

eBlot™ L2 allows fast and efficient transfer of 2 midi or 4 mini gels at a time. The setup takes less than 2 minutes and avoids the mess of a traditional wet transfer with our easy to assemble dry sponge sandwich.

Flexibility

Our customizable protocols allows optimal flexibility for your research. The unit comes with standard protocols designed to transfer larger proteins (up to 427 kDa) or opt for a shorter transfer time for smaller size proteins (up to 100 kDa).

Gold Standard Wet Transfer Efficiency

Side-by-side comparison data between eBlot™ L2 Standard Method and traditional wet transfer using GenScript 4-12% Precast Gel.

	eBlot™ L2: 2x5 min (NC membrane, 0.22 μm)	Traditional wet transfer: 100V, 60 min (NC membrane, 0.22 μm)
EGFR ~150 kDa
β-actin ~42 kDa
GAPDH ~36 kDa
Histone H3 ~17 kDa

Target protein	Samples	Primary Ab	Secondary Ab
EGFR	HEK293 Cell Lysate, left to right: 20ug, 10ug, 5ug, 2.5ug, 1.25ug, 0.62ug, 0.31ug, 0.15ug	Recombinant Anti-EGFR antibody, mAb, rabbit (Abcam, ab52894), 1:1000	Anti-rabbit IgG (HRP) (GOAT) antibody peroxidase (Rockland, 610-103-122), 1:10000
β-actin	HEK293 Cell Lysate, left to right: 40ug, 20ug, 10ug, 5ug, 2.5ug, 1.25ug, 0.62ug, 0.31ug, 0.15ug	THE™ beta Actin antibody, mAb, mouse (Genscript, A00702), 1:5000
GAPDH		GAPDH antibody, mAb, mouse (GenScript, customized), 1:1000
Histone H3		Histone H3 antibody, pAb, rabbit (GenScript, customized), 1:1000

eBlot L2 outperforms other market available transfer methods, particularly for the large molecular weight proteins.

Full Protein Size Coverage

Simultaneous Transfer of High, Medium and Low-MW Proteins via eBlot™ L2 Standard Method.

ATM - 370 KDa
EGFR - 175 kDa
β-Actin - 42 kDa
RPL17 - 21 kDa

Precast gel: SurePAGE™ precast gel, 4-12%

Samples: HEK293 Cell Lysate, left to right: 30μg, 15μg, 7.5μg, 3.75μg, 1.875μg, 0.937μg, 0.468μg, 0.234μg, 0.117μg

Transfer: eBlot™ L2 Standard Method (2x5 min)

High sensitivity

Serial gradient dilution of recombinant proteins with different molecular weights, then transfer with eBlot™ L2. This data shows that the eBlot L2 successfully transferred the 0.008 ng protein band.

Samples	S1	S1	S2	S3	S4	S2	S3	S4	Unit
120 kDa	4	4	0.8	0.16	0.03	0.8	0.16	0.03	ng
80 kDa	1	1	0.2	0.04	0.008	0.2	0.04	0.008	ng
60 kDa	2	2	0.4	0.08	0.016	0.4	0.08	0.016	ng
50 kDa	1	1	0.2	0.04	0.008	0.2	0.04	0.008	ng
40 kDa	2	2	0.4	0.08	0.016	0.4	0.08	0.016	ng
30 kDa	1	1	0.2	0.04	0.008	0.2	0.04	0.008	ng
20 kDa	8	8	1.6	0.3	0.06	1.6	0.3	0.06	ng

eBlot™ L2 Bundle Promo

eBlot L2 Device Starter Kits	Starter Kit A	Starter Kit B	Starter Kit C
eBlot™ L2 Fast Protein Transfer Device	1 * L00981
eBlot L2 Transfer Basic Kit, midi	1 * L01015-30
SurePAGE™, Bis-Tris, 10 x 8	1 Box (10 pk, Optional Concentration & Wells)
MOPS Running Buffer Powder/ MES Running Buffer Powder	1 * M00138 or M00677
PVDF Membrane Midi, 0.22 μm, 8.5 x 13.5 cm		1 * L00990
PVDF Membrane, 0.22 μm, 8 x 9 cm			4 * L00735
Description	For 30 midi or 60 mini transfers with standard method	For 30 midi transfers with the standard method (including PVDF midi membranes)	For 60 mini transfers with standard method (including PVDF mini membranes)
Quick Order	Get 20% Off for Starter A	Get 20% Off for Starter B	Get 20% Off for Starter C

Got old equipment? Enjoy discounts with trade-in/trade-up

eBlot L2 Transfer Packs and Kits	Basic Transfer kit	Transfer Kit, midi, PVDF	Transfer Kit, mini, PVDF
eBlot L2 Transfer Basic Kit, midi	1 * L01015-30	1 * L01015-30	1 * L01015-30
PVDF Membrane Midi, 0.22 μm, 8.5 x 13.5 cm		1 * L00990
PVDF Membrane, 0.22 μm, 8 x 9 cm			4 * L00735
Total List Price	$360	$465	$620
Description	For 30 midi or 60 mini transfers with standard method	For 30 midi transfers with the standard method (including PVDF midi membranes)	For 60 mini transfers with standard method (including PVDF mini membranes)
Quick Order	Add products with selected Item Nos. and quantities to the cart; PVDF membrane coupon can be activated directly.

eBlot™ L1

eBlot™ L1 Video

Watch eBlot™ L1 Video

Watch eBlot™ setup Video

eBlot™ L1 Application data

	Wet Transfer	Competitor A	Competitor B	eBlot™ L1
EGFR (170 KD; from left to right: 20 µg, 10µg, 5µg, 2.5 µg, 1.25 µg)
Taq (93 KD; from left to right: 125 ng, 62.5 ng, 31.2 ng, 15.6 ng, 7.8 ng)
GAPDH (36 KD; from left to right: 10 µg, 5 µg, 2.5 µg, 1.25 µg, 0.25 µg)

Comparative blotting efficiency with various transfer systems. Three different proteins were transferred on to PVDF membranes using traditional wet transfer, Competitor A, Competitor B, and GenScript's eBlot™ L1. The quantities of proteins loaded ranged from 1.25 µg- 20 µg for EGFR, 7.8 ng- 125 ng for Taq, and 0.6 µg- 10µg for GAPDH. For all three proteins, the eBlot™ L1 offered superior transfer efficiency compared to other systems. With its high efficiency of transfer, protein concentrations as low as 7 ng for Taq (lane 5), and as high as 20 µg for EGFR(lane 1) could be clearly visualized.

Customer Testimonials

eBlot™ L1 helped Dr. Antoni Barriento's lab transfer their 1200 kD protein in 20 minutes.

"This is the first time we have used one of these fast-transfer instruments. I decided to test this one because uses a wet transfer system, rather than semidry, which yields less efficient transfers of large complexes even when using classical transfer systems."

"Our major interest was to minimize the transfer of assembly mitochondrial oxidative phosphorylation complexes and supercomplexes which are large membrane-embedded macrostructures of sizes that range between ~200 kDa and several megadaltons."

-Dr. Antoni Barrientos,Ph.D.
University of Miami Miller School of Medicine

*Figure prepared by Dr. Alba-Timon-Gomez and Dr. Antoni Barrientos. University of Miami School of Medicine

Blue Native (BN)-PAGE analysis of mitochondrial respiratory chain complexes and supercomplexes using an eBlot™ fast and efficient wet transfer from GenScript.

Cellular extracts from HEK293T cells treated with non-targeting (NT) or siRNAs targeting the indicated genes involved in complex IV assembly, prepared in the presence of digitonin, were loaded onto a 3-12% native gradient polyacrylamide gel and separated by BN-PAGE. Following electrophoresis, the proteins were transferred to a pvdf membrane using in 24 minutes, using the Blot L1 protein transfer system from GenScript. To visualize mitochondrial respiratory chain complexes and supercomplexes, the membranes where subsequently decorated with antibodies against COX5B (a complex IV subunit), CORE2 (a complex III subunit) and SDHS (a complex II subunit).

Service Plans

With a dedicated team of highly skilled and experienced engineers, GenScript delivers comprehensive warranty and maintenance services to ensure you get the best possible experience with our instruments.

Standard Warranty

One year warranty coverage of instrument repair, parts replacement, and technical support services, as frequent as needed.

Extended Warranty

Additional year-by-year extension coverage of instrument repair, parts replacement, and technical support services, as frequent as needed.

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