Protein Webinars

Codon optimization: Why & how to design DNA sequences for optimal soluble protein expression

Have you struggled with low protein expression levels in your experiments? This webinar will explain the principles of codon optimization and explore case studies showing how it improves protein expression up to 100-fold.  Research has revealed dozens of DNA sequence features that influence the efficiency of each step required to achieve soluble target protein expression. We will review the critical publications that inform GenScript's patented algorithm and the data showing how our algorithm compares to our competitors. We will look at peer-reviewed papers that employed codon-optimized synthetic genes for heterologous protein expression in different host systems, including bacteria, yeast, plant, and human cells. Finally, we will see how GenScript's codon optimization can provide clever solutions to molecular biology problems in specialized applications.

Presented by: Rachel Speer, Ph.D. Originally aired October 29, 2014

Optimizing soluble protein expression: codon optimization, RBS design, and expression vector design

Do you wish there were an easy way to optimize protein expression? This webinar will introduce you to three powerful strategies that can make your research more efficient: codon optimization, ribosome binding site design, and expression vector selection. We will describe the principles underlying these strategies, review case studies showing how big an impact they can have, and walk through how easy it is to use GenScript's free computational tools and best-in-class gene synthesis and cloning services to get optimized expression constructs.

Originally aired November 11, 2015

Expression vectors: how to choose, or customize, vectors for gene & protein expression

Do you make new DNA constructs only using the old expression vectors you're most familiar with? This webinar will help you make your experimental design more efficient and powerful by learning how to select or design an expression vector that is optimized for your experiments. We will walk through how to read a plasmid map and what key features to look for in an expression vector depending on your research goal. Using case studies from published literature, we'll discuss why and how you might want to make custom changes to elements already included in commercial available vectors (e.g. RBS or tags). Along with handy reference guides for popular vectors used in different eukaryotic and prokaryotic species, this webinar will introduce you to GenScript's gene synthesis and cloning services that can help you get expression-ready clones most efficiently to accelerate your research.

Presented by: Rachel Speer, Ph.D, Technical Writer, GenScript

Clone less, know more: efficient expression optimization of proteins and pathways using the RBS calculator

Maximizing a heterologous protein's titer can be a balancing act between under- and over-expression. Too much: the protein aggregates. Too little: scale-up is not feasible. This challenge becomes enormous when multiple proteins are involved, for example, when expressing a multi-subunit therapeutic or engineering a multi-enzyme metabolic pathway. It's costly and honestly quite boring to screen combinatorial libraries.

To solve these challenges, this webinar introduces the computational design of synthetic DNA to systematically optimize expression levels in one- or multi-protein genetic systems, while performing the fewest number of experimental measurements. We introduce the physics behind the RBS Calculator, a thermodynamic model that predicts a mRNA's translation initiation rate using only its sequence. We then show how to use the RBS Library Calculator to design small RBS libraries that search large expression spaces. Instead of screening 10^8 variants, our computational design approach requires only 10 to 100 characterized constructs. We present several case studies showing how to optimize protein titers and engineer multi-enzyme metabolic pathways.

Presented by: Prof. Howard Salis, Penn State University

Optimizing conditions for recombinant soluble protein production in E. coli

Are you looking for ways to optimize protein expression? Then please join us for this interactive webinar geared to address the optimization challenges in recombinant bacterial protein expression. There are several variables in the E. coli protein production process that can influence the desirable end result i.e. generation of high yields of biologically active target protein. Our experience and in-house capabilities can help you overcome this bottleneck in recombinant protein production.

Presented by: Keshav Vasanthavada
Originally aired May 8^th and June 24^th, 2014

Fusion partners for recombinant soluble protein production in E. coli

Are you working on an E. coli expression project and looking for ways to generate soluble protein? If so, this interactive webinar may be for you. Target protein solubility is one of the most important end goals of a recombinant protein production campaign yet it remains a significant bottleneck. While there are various ways to optimize expression in order to produce soluble protein, a fusion partner based approach can be very useful. This webinar reviews the practical aspects of a fusion partner strategy. Topics include but are not limited to - the need for protein solubility, the relationship between solubility and protein functionality, use of soluble fusion partners, examples of commonly used partners, identifying novel fusion partners and pros and cons of a fusion partner approach.

Presented by: Keshav Vasanthavada Originally aired December 3, 2014

Chaperone co-expression strategies for recombinant soluble protein production in E. coli

Recombinant protein expression in E. coli often results in the formation of insoluble aggregates called inclusion bodies. In vitro refolding techniques can be adopted to recover recombinant protein but the biological activity of the target is often lost during this process. While there are several approaches to bypass inclusion body formation, chaperone co-expression can be a good option. Chaperones assist newly synthesized proteins fold into their native states while shielding exposed hydrophobic patches of non-native proteins to prevent their aggregation. In this webinar we will present some popular chaperone co-expression strategies that could help you achieve higher yields of recombinant soluble protein.

Presented by: Bo Wu, Ph.D, Senior Scientist, GenScript

Analyzing antibody sequence for recombinant antibody expression

Is your antibody design strategy becoming a bottleneck in achieving high levels of recombinant antibody expression? If so, this webinar may be for you. In this session we will review some antibody basics and see how sequence information correlates with its heterologous expression in mammalian cells. We will also provide suggestions on how to efficiently evaluate your antibody sequence in order to come up with the ideal cloning strategy, prior to expression testing. Topics include but are not limited to - identifying CDRs, analyzing the variable and constant regions and investigating unusual sequence features.

Presented by: Hangxing Yu, Ph.D, Senior Scientist, GenScript

Efficient stable cell pool/cell line development for large-scale antibody and protein production

Interested in rapid and efficient stable cell pool/cell line development for large-scale production of your recombinant antibodies and proteins? If so, this webinar may be for you. In this session, we will review some basic information about generating a stable cell pool/cell line using the Glutamine Synthetase-based system. We will also compare the usability of Glutamine Synthetase Expression Technology system alongside the Dihydrofolate Reductase (DHFR) system for different applications. Topics such as how to efficiently screen stable pool/stable clones for increased expression, how to monitor the consistency, reliability, robustness and potency of products during scale-up will also be discussed.

Presented by: Hangxing Yu, Ph.D, Senior Scientist, GenScript Originally aired October 22, 2015

Recombinant protein applications: choosing the best cytokine, growth factor or chemokine for your experimental system

Are you treating cells in vitro? Do your in vivo studies investigate changes in the immune response or tumor growth? If so, choosing the right recombinant proteins for your experiments is an important step that can affect the accuracy and consistency of your results. This webinar will take an in-depth look at what to look for when deciding which recombinant proteins to use in your experimental system. Topics that will be covered include categorizing different types of cytokines, growth factors and chemokines based on their function and laboratory application, defining which specific cytokines and/or growth factors are optimal for activating various cell types and pathways based on peer-reviewed data, and outlining best-practice laboratory techniques for using cytokines and growth factors in vitro and in vivo based on their intrinsic properties.

Presented by: Matthew Riolo, Ph.D. Originally aired October 16, 2014