GenWand™ Double-Stranded DNA Synthesis Service

Why GenWand™ dsDNA Service

Made via plasmids based production process
2-10 kb
µg to g level production
Comprehensive QC to ensure minimum impurities
Built in endotoxin removal process and guaranteed < 10 EU/mg Endotoxin
Quality grades designed for different research stage (RUO, Preclinical, Basic GMP Grade)

dsDNA

Starting at 50µg/item
Deliver in as fast as 3 weeks

Length (bp)	Research Grade	Preclinical Grade
2000~5000	Starting from $1,800/item	Starting from $2,050/item
5001~10000	Starting from $2,200/item	Starting from $2,500/item

Test Specifications	Detection Method	Release Criteria	Research Grade	Preclinical Grade
Purity	Agarose gel electrophoresis	Single band	✔	✔
Sequence accuracy	Sanger sequencing	100% sequence alignment	✔	✔
Optical density	Spectrophotometer at 260 nm/230 nm	≥ 2.0	✔	✔
Optical density	Spectrophotometer at 260 nm/280 nm	1.8~2.0	✔	✔
Bioburden	Incubation in TSA plate	No colony formation	-	✔
Endotoxin	Qualitative TAL assay	< 10 EU/mg	-	✔
Protein Residue	Micro BCA Protein Assay Kit	≤50µg/mg	-	✔
Quantitative purity	Agilent 2100 Bioanalyzer	≥90%	-	Add-on
pH	pH meter with pH probe	Depending on buffer pH	-	Add-on
Conductivity	pH meter with conductivity probe	Depending on buffer conductivity	-	Add-on

Applications

CRISPR based gene insertion, replacement, or correction

Mechanism of CRISPR HDR based gene editing

CRISPR/Cas9 technology is commonly used to create precise double stranded breaks (DSBs) at target DNA sites. The guide RNA (gRNA) recognizes the protospacer adjacent motif (PAM) sequence on the target DNA after forming complex with Cas9, then Cas9 exerts its endonuclease function to cause DSBs. This triggers two mechanisms for repair: one is non-homologous end-joining (NHEJ), which introduces mutations in the DSB site. The other mechanism is homology directed repair (HDR) which enables the donor DNA to be inserted at the break site and create gene knock-ins.

Resourcess

Flyer:

HDR Template Flyer

Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance. Orders can be placed online, over the phone, by email or fax. Please have your payment information (credit card, purchase order or bank information) ready.

Email: gene@genscript.com

Phone: 1-877-436-7274 (Toll-Free); 1-732-885-9188

Fax: 1-732-210-0262