UMAG_05232 cDNA ORF clone, Ustilago maydis 521

Gene
Clones
gRNAs

Summary

Gene Symbol	UMAG_05232
Entrez Gene ID	23565179
Full Name	Adenylate cyclase
Gene Type	protein-coding
Organism	Ustilago maydis 521

mRNA and Protein(s)

mRNA	Protein	Name
XM_011389967.1	XP_011388269.1	Adenylate cyclase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis.
Kämper J, Kahmann R, Bölker M, Ma LJ, Brefort T, Saville BJ, Banuett F, Kronstad JW, Gold SE, Müller O, Perlin MH, Wösten HA, de Vries R, Ruiz-Herrera J, Reynaga-Peña CG, Snetselaar K, McCann M, Pérez-Martín J, Feldbrügge M, Basse CW, Steinberg G, Ibeas JI, Holloman W, Guzman P, Farman M, Stajich JE, Sentandreu R, González-Prieto JM, Kennell JC, Molina L, Schirawski J, Mendoza-Mendoza A, Greilinger D, Münch K, Rössel N, Scherer M, Vranes M, Ladendorf O, Vincon V, Fuchs U, Sandrock B, Meng S, Ho EC, Cahill MJ, Boyce KJ, Klose J, Klosterman SJ, Deelstra HJ, Ortiz-Castellanos L, Li W, Sanchez-Alonso P, Schreier PH, Häuser-Hahn I, Vaupel M, Koopmann E, Friedrich G, Voss H, Schlüter T, Margolis J, Platt D, Swimmer C, Gnirke A, Chen F, Vysotskaia V, Mannhaupt G, Güldener U, Münsterkötter M, Haase D, Oesterheld M, Mewes HW, Mauceli EW, DeCaprio D, Wade CM, Butler J, Young S, Jaffe DB, Calvo S, Nusbaum C, Galagan J, Birren BW
Nature444(7115)97-101(2006 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following UMAG_05232 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UMAG_05232 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OUd06517	XM_011389967.1 Latest version!	Ustilago maydis 521 Adenylate cyclase (UMAG_05232), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UMAG_05232 ( XM_011389967.1 ) cDNA ORF clone, Ustilago maydis 521 -> XP_011388269.1 Ustilago maydis 521 Adenylate cyclase (UMAG_05232), partial mRNA.

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CloneID	OUd06517
Clone ID Related Accession (Same CDS sequence)	XM_011389967.1
Accession Version	XM_011389967.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1522598400000
Organism	Ustilago maydis 521
Product	Adenylate cyclase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026481). COMPLETENESS: incomplete on both ends.

ATGCTCTTTA CCATGCAATT CACCACTCGA TCCACCGTGG CCTCGCCCGA GCAGCAGCAC 
CAACACCAAC AGCGCAGTAT CTCGTACTCG GATATCGAAC TTGGTCTTGA GCGCATCAGC 
TCTCGCGACA GCAATGGCAG CTCCAACTTT ACGCACAGAG CCTATCCTCC ACCGCTATCT 
CAGCAATATG ACGACACATC CACCAACTCC TTTCATTCAT CGCAGCCAGA CATCACAGCC 
TCTTCTTCAA CTTTGTCGTC TCGCCTCGTG AGCGCCAACT ACTCCCGTCC TCGGTTCGAG 
CATGCGCACA CTCAACCACC CACACCCGAC CAAGACCGCT CCAGCTCCGG CTCCGGCTCC 
GGCTCCGGCT CTCGCTCCTA CTTTCCCGCC AACTCGCATT CCGACTCTTT GCCCGGTCCC 
AGCACTCATA GCATCAGCCC TTCCTTTGAC GAGGACGAAC TCCGTCAGAT CATGTCGCAC 
ATTCCAGCTA ACCAAGCCAC GTCCAGCTCG GATGGCGATG TAGGCAAGGC CGTCCAGTCT 
GCCAACCATC AGGACATCAG TCCGTTCCTC TTCCAGAGCG AGAACGCAGC TCCCTTTTCC 
TCCTCCCACT CCAACCGTAC CAGTGTCAAT CCATCCGCAG CCTCGACCGC CAGCCCGTCG 
ACTTCGGCGG CAACCAGGAC ACGTCCGCGC GGGGGCACCA ACGCCTCGCA GTACAACACC 
CTAGACACTA GCTTCGGTAG CATCGATCGG CCTGGCCTTT CTTCTTCCCG CTCTCAGTAT 
TCATTGCGCC CACAGACCCC ACCCTCGGCG TCCACTTCCA CTAGCACTCT CAACGGCTCC 
AAGGACACGC ACGCTTCTGC CGTCAAGAAG ACGCGCAATC CGTTCGGCTT TCTCAAAAAG 
AAGTCCTCGG CCCACTCAAA TGCTTCCTCG AATCACCCCA CGCGCCACGA CATCGGCTCG 
GTCTCTTCGC TCTCGTCGCG CTACGGTCCC AACGCGGCGG CCAACGTCAA TCCGATGCGC 
CCACCTGCCT GGCTCGATAA TCACTGCACA CTTGCCAACT CCAACTCGCC TTCTTCGGCT 
TCGCTGCGCT CTCACTACCA CCAGCCTCCT GCCTCTTCGA ATCCCCCTCC TTGGCAGAAC 
CCCCTCGTGT CTCGTGCAGA CTCGACTCCT TCCGCCATGA GTCTCGAAGA TGAGGTCGAG 
GCTGAGCATC ATCTCAAGAA GGACCCTCGC AAGCGCATCA AAGGCGTTCG CCATCACCTT 
GCAAAGACCA CCAAGCCCGG CGAGGACGCC GACTCGGCAC GCGACCCTGC CTTTGCAGCC 
CAGAGCCAGT CCATCGAACA AGAGGTCGAA CTCTCGTTGG ACATGAACTT TGACCAGCTC 
GATGACTTTG TCGACACCAA CGCAGCGCGC CAGCGGCTCC AGGGCTCGAT CACTGAGTCT 
GCCAGTCCTT CCGAGCATCG CTCACCAAAC GGAAGCGAAG CCGGCGTGTA TCGCTCGCCA 
TCCCCTTCCC AGACTCCCAT TGCCGAACGC CAGACTTCGG TCACTTCTAC CGTCGAATCT 
CCCTCGCACG CCTCGGAAGC CTCGCTCGCA CCCTCGGGCT CTCTGCGAAC CCCGAGTCGC 
ACTACTGCGT CTACCTCTAC CTCGTCCGCT TCCACCGTAC TCAGCGACCG TCTTCCTTCG 
CAGGTCAACA TGCTTCCTCG CAATAGCGTC CCCAGGCTCA GCCTAGCCGA GATGCAAAAC 
TACCAGTCGC TCCGTAAACT CTCGAACAAC CTCATCGACA TGTCCCAGAC GCAAAACCCT 
TCCGCTATGG GCGCATCCTA CCGCCGCGGA TCCATCGCAG CCGCCCAAGC ACCTGTCGAC 
GCGCCTCAGC TAGGTGTCGC TCCTCGTACT GACTCTGAGC TCTCCGATCG CAAGGACAGC 
GTAGTCTCTA CCCACAGCAT GCGCTCCAAC CATAGCGGCA TCTCGCCCAA GACCTCATAC 
GCCAACCTGC CCAGTGTCAT TCAGGAGCGT CAGAAGCCGG CTACTGCACT TCCCTCCGCC 
GCGAATTGGA CATCATCCAT CACCAGAGAC AAAACGGCCA ATGGACATGC TGACCACGCC 
TACCAATTTC CGCCCGCAAC TGAGTATCAG TCCAACTTGC TGCTCAGCGT GCGCAAGTCG 
AGTGCCAGCT CGGGGCAGGA GCCTAGCTCC AGCTGGATGG CTCCCGACAG TTGGGCCGTA 
CAACCGGACA AGATGCGCGA CTATCTTCGC GACGATAATG TCGGCGAGGA GGAAGACGAC 
GACGACGACC AGCACCAAGC CAGAGCCGCT TTGGCCACCG ATGGCAAGAG GCGCGGTTCC 
AGCTCCGGTA TCAGCTCGAC GCATGCATCG AGCATGTTCC GCACCTCATC CACCGACCCT 
TTCAAGAAGA CTGCCTCGCT TGCTGGCTCA CGTCGCGGCA CGGACGACTC GGTCGATCCG 
CTCACCGCTT TGCCTCCATT GCCAGGCTCC AAGTCGGTGG ACGAGGCAGC AGCCAACAAG 
GTCGATGTAC TCCAGCAGAC CAATAATCTC GCTCAATCGG CTCTGGTGCA ACAGCAGTCT 
CAATCCCAGA ACCACCACCA GCCCAGCCCC AACGTCCGTC CCACCTCGAG AGGCGGTGCT 
GGTGCCCACA TGTTTGCCTC CGCCGGCGCT TCCGCTGCAG CAGCTGCTGC GGGCAAGCTT 
GGTTTACATC GTCCATCTAA ACATCGTATG AACGCGCGAC CCAATACCGC AGGCTCCGTT 
GGTGCGACCC GACCTTCCAC CACCACGCTC GGCTCGACCC TCTCGGCAGA GGACGACACA 
AGCATCAACG GCTCCATCCG ACGCGACGGC CATCCGCTCA AGCGCTCCGC AACTGCCAAC 
ACGAACAACG CCACGGGAAC TTTGCCTCGC AACCACTTCA TCCGCGTCTA CAAGACGGAC 
GGCACCTTTG CAACGCTCTC GTGCTCACTC GTCTCGACGG CAAACGAAGT ACAGACAATC 
CTCGCGCGCA AGAGCCTCAC CACCGAATCG GCCGCATACA GGCTTTTTGT GCGGGACAAG 
GGCTCCGAGA GACCGCTGGG GATCTCGGAC AAGCCTTCTC AGCTCCAGCG TCGTCGTCTC 
ATTCAGGCAG GCTACACCGA GAACGATGGC TTGGAAGATA TGGGCAGAGA CGATTTGTCA 
TACCTGCTTC GCTTCGTCTT TCGCCCCGAC AGTGTTCCCA CCTTCGACTC GGAATCCATC 
GGCCACAGCG AGCACACATT CCAGCATCTC GATCTGCACA GCCGCAATCT CGAAATGGTG 
CCCATCTTTC TGTATAAGCA TGCCGACTGG ATTGTTAGCC TCGATCTGTC AGGCAATCCC 
ATGTCTGATC TTCCGCTCGA CTTTGTACAG CTCTGTTCAA GCCTGCGAAC TCTGCGACTC 
TCAAACTTGG CGCTCAAGCG CATCCCGCAA AGTGTCCGGC ACAGCGAAAC CTTGACGCAC 
CTCGACGTAT CCAACAATCG AATTGTCGAG CTCGCGCATG TCAGTCTCGA TCTTATTCCG 
GAGCTCATGT CGCTCAAGGT GCAAAACAAT CGTCTCTTTG ACTTGCCCTC ATACTTTTCC 
AGCATCAGCA CGCTGAGGAA CCTCAACATC TCTAACAACC GCTTTGAAGA ATTCCCCAAG 
GTCATCTGCG ACGTCCCTTC GCTGGTCGAT CTTGATGTGT CATTCAATTC GATCACTGAA 
TTGCCCGCCG AAATCGCCAA CCTCATCAAC TTAGAGCGCT TCATCCTAGC CGGCAACGAG 
CTGGAAAAGC TGCCGGACAG CATGAGCGAG CTCGTCAGCT TGCGCACCAT CGACCTGCGT 
CGTAACAAGG TGCAGGACGT TTCGTCGCTG CTTGGTCTTC CACGCCTTCA AAACATCCAG 
GCCGAGAGCA ACAACATCAA GTCGTTTGAA GCTACACTGG GTCCGCAACT CACTCAGGTC 
GAGCTCGGTC GCAACCCACT CAGCAAGGTG CGCATCGCTG CTCTCACCAC GTGCGATCTC 
ACCTCGCTCG ACCTGTCGTC GACCAACATG ACGCGGCTCG AGGAAGGTCT CTTCCCGCAG 
CTGCCTGCGC TCGTCAAGCT GACGCTCGAT GGCAATCAGC TCGTTGTGCT GCCCGACACG 
CTCGGAGACT TGAAGCGACT CGAGATGCTA TCATGCAGCA ACAATCTGCT TGCTACCCTC 
CCCGAGTCGA TCGGTGATCT CAAGGCGCTC AAGGAGCTGC TTGTGCACAA CAACAACCTC 
AAAACGCTTC CACAGACCTT GTGGCTCTGC GAGAGCCTCG CACACATCAA TCTCAGCTCC 
AACTTGCTCG AATCGTTCCC AGCAGTGCCT GACATTCGCA CCGATGCTTC CGTTGGAGAT 
GCGGCTGCAG CTGCCGGCAC TTCGGCCGTT ATCGCTGCAC GAAAGGGATC TACAAGCTCG 
TCGCTGACGC ACAGGTCTAA CACTGGTGGC GCCAATGGGA ACATCAATCT CTCTACGCCC 
TCCGAGGTCT TTGTGGCGCC GCTCTCGCTC AGCCTGCAGA AGCTGCGTCT GGGAGACAAT 
CGTCTAGGCG ATGACGTTTT CAGTGTCCTG TCCGAGCTCA CCTCGCTCGA GGTGCTCAAC 
CTCAGCTTCA ACGAGATCTT TGAGATTCCC GATTTTAGTC TCCAGACGCT CACCAAGCTG 
CGTGAGCTAT ACATCAGTGG TAATCAGCTT AGCACGATTC CTTCCGACGA TCTTGTGGTG 
CTACAAGAGC TGCGCATCCT CCACCTCAAC TGCAACAAGC TCACTACTCT CCCCACCGAA 
CTTGGCAAGC TCAAGAAGCT CGCCAACCTG GATGTCGGTA ATAACGTGCT CAAGTACAAC 
ATTGCCAATT GGCATTACGA CTGGAACTGG AACATGAACC CGGAGCTACG CTACCTCAAC 
CTTTCCGGCA ACACGCGTCT CGAGATCAAG ACCAAGCTGA GCGATATGGG CTTCACGCGC 
AAGTCCAACA TCTCGGACTT CAGCCGCTTA ACCAGCTTGC GCATGCTGGG TCTCATGGAT 
GTCACAATGC CATTGCATTC AAATGCCACG CCGGATGAGT CGGACAACCG CCGTGTGCGA 
ACCTCGCTCT CGCAGGTCAA CGGAATGGCT TACGGAATCG CCGACGCACT CGGCAAGCAC 
GACAACTTGA GCGTCATCGA TCTTGTGATT CCAACATTCC GTAAGGACGA AGGAGAGTGC 
ATTTTTGGTC TATTTGACGG TAGGGGCCAC GGTGCACATG TTGGCAGCCG TATCGCGCAT 
CACCTGGCCG AATGGAGTGG ACATCGTCTA TCGTGGGAGT TCCAGAAGCA CCAGAATGAA 
ATGACCGCCG AACCTGTTTC GGTTCCGGAT GCGCTGCGCC GCGCCTTCCT ACGCCTGCAG 
AAGGACTACG CCGACGCGCT CATCAACGAT GGCAGCCGCA AGCTCTCTGA AGCACACGCA 
GAGGCGGCTG CGGATGTGAC GCGAAGCTCT GCGCCTGCGA TTGCGGCCGC CTCCAACAAG 
CATGACTGGC GCGCCGGCGC TTCCGCGATT TTAGCGTACG TGGTCGACCA CACGCTCTAC 
ATTGCTAATG CGGGTGATGC TCTGGCGGTC ATGTCGCGCA ACGGTGGCAC AGCACATCTG 
ATCAGTAACA AGCACGAGCC GTTTGACCGT GCCGAGATCG AGAGGATTCG ATCGGCGGAA 
GGATGGGTCT CGCTGCGTGG CTATGTCAAC GACATGCTCG ATGTCTCGCG TTCGTTTGGC 
TACTTCCATC TGTTCCCGAT TGTCAATGCT GCACCTGCGG TGACGACGGT GCAGTTGACT 
GACTCGGATG AATTTGTGAT TATCGCCAAC CGCACGCTGT GGCAGTATGT GTCGTACCAG 
ACCGCGGTCG ACATTGCACG TACACAGCGA AACGATCCTA TGATTGCAGC GCAGAAGCTG 
CGAGATTTTG CCATCAGCTA CGGTGCCGAG GAGAGCATCA TGGTCATGGT GATCTCGGTG 
GGTGATTTGT TCTATCGTTC AGATCAGCGT AACGGCGGTG GACTCAACTT TGCGTCGTAC 
AAGAACAGCG ACGCCATCCA AAAGGCAGGA CGTCGATTCC GCGAGGAGCT GCCTGGCGAT 
CGCACGCTGG CGAGACTGGA CCGTGAAGTG GCACCTCCCA TCGGACAGGT GGCGCTGGTG 
TTTACGGATA TCAAGAACTC GACGTCGCTG TGGGAGACCA ACAATGGCAT GCAGACGGCG 
ATGCGATTGC ACAACTACCT GTTGCGACGA CAGTTGCGCA CTATTGGGGG TTATGAGGTC 
AAGACGGAAG GCGATGCATT TATGGTTTCA TTCCCCTCGG TCAGTGCAGC TCTGCTGTGG 
TGTTTTACAG TACAGCAACA GCTGCTGCAA GAGGACTGGC CGCGCGAGAT CTTGGACAGC 
GAGGACGGCA AGGAGGTGTA CGATCAGTCG GGCGAACTCA TTCATCGAGG TCTGTCGGTG 
CGCATGGGCA TTCACTGGGG ACGGCCAGTG TGTGAGGCGG ATCCGATCAC GAGACGAATG 
GACTACTTTG GACCGATGGT GAACCGTGCT GCTCGAATCA GCGGTGCAGC CGATGGCGGT 
CAGATCTTGG CGAGCAAGGA CGTAATCAAG GAGCTGCAGG GCCTATTGGG TACGTTTGAC 
GAGTCGTCGA CAGCCGGAGG TGCGGGCGGA GAAGGTGAAA ATCTAGAGAA GACCGAGGAG 
GAGCTAGACG AGGATGCGTT CCGACTGCTC AACCCGAACG TCAGCCGCGA TGTGGTGCTG 
CTACGTAGGA TGGGTTTTGG TCTATCGCAG CTGGGAGAGC GCAGACTCAA GGGTTTGGAG 
ACGCCCGAGA TGCTGTGGCT TGTCTATCCG AAGCAGTTAG CAGGACGTCT GGAGCAGGCC 
AAGACCGACG ATGCACCTGA CGCACCCACG GCGCAAGTGT ATGAGCCGAC AGTGCAGCTG 
CTAGATATCG AGGATGTAAA GCAAGTGGGC ATGCTCTGCT TGCGACTCGA GTATCTGTCC 
AACTCTACGG TCTGTCCTGG CATCTTTGCT GCCAAGGATG AAGCGGATCG ATCGCAACCT 
TCGACGCCTC TGGACGATAA CGGACGGAAT CCGATCGACG GCCACGGAAC GGCGGTGCCC 
CTGCTGTCAC ACCAGGCGCG ACGCAAAGGG GTTGAGGCGA TGTTGACGAT GCATCCCGAG 
CTGCTCATCT ACAGCATCCG AGACGATGCG ACGGATGAAG AGTTGGCGGG CATCTTGGAT 
CAGCTGACGA CGCGGATCCA GAACGCCGTG TCTTCGCTCA TGCTGAATAT GTTGCGGGAC 
AAGACGGCCA ACGGTACCAA GGAACTTGGC GTGGATCCTG GGGTGCTCGA GTTGTTGATG 
GGCTTGCTTT CACAGCCGCC TCCGCGAGCG TCAACGTCGG CGTTGAGTTT GCCCAGCCCA 
CGGACGAGCC CTCGCAACAG GCTGTTGGAG CTGGTTCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011388269.1
CDS	1..7482
Translation	MLFTMQFTTRSTVASPEQQHQHQQRSISYSDIELGLERISSRDSNGSSNFTHRAYPPPLSQQYDDTSTNSFHSSQPDITASSSTLSSRLVSANYSRPRFEHAHTQPPTPDQDRSSSGSGSGSGSRSYFPANSHSDSLPGPSTHSISPSFDEDELRQIMSHIPANQATSSSDGDVGKAVQSANHQDISPFLFQSENAAPFSSSHSNRTSVNPSAASTASPSTSAATRTRPRGGTNASQYNTLDTSFGSIDRPGLSSSRSQYSLRPQTPPSASTSTSTLNGSKDTHASAVKKTRNPFGFLKKKSSAHSNASSNHPTRHDIGSVSSLSSRYGPNAAANVNPMRPPAWLDNHCTLANSNSPSSASLRSHYHQPPASSNPPPWQNPLVSRADSTPSAMSLEDEVEAEHHLKKDPRKRIKGVRHHLAKTTKPGEDADSARDPAFAAQSQSIEQEVELSLDMNFDQLDDFVDTNAARQRLQGSITESASPSEHRSPNGSEAGVYRSPSPSQTPIAERQTSVTSTVESPSHASEASLAPSGSLRTPSRTTASTSTSSASTVLSDRLPSQVNMLPRNSVPRLSLAEMQNYQSLRKLSNNLIDMSQTQNPSAMGASYRRGSIAAAQAPVDAPQLGVAPRTDSELSDRKDSVVSTHSMRSNHSGISPKTSYANLPSVIQERQKPATALPSAANWTSSITRDKTANGHADHAYQFPPATEYQSNLLLSVRKSSASSGQEPSSSWMAPDSWAVQPDKMRDYLRDDNVGEEEDDDDDQHQARAALATDGKRRGSSSGISSTHASSMFRTSSTDPFKKTASLAGSRRGTDDSVDPLTALPPLPGSKSVDEAAANKVDVLQQTNNLAQSALVQQQSQSQNHHQPSPNVRPTSRGGAGAHMFASAGASAAAAAAGKLGLHRPSKHRMNARPNTAGSVGATRPSTTTLGSTLSAEDDTSINGSIRRDGHPLKRSATANTNNATGTLPRNHFIRVYKTDGTFATLSCSLVSTANEVQTILARKSLTTESAAYRLFVRDKGSERPLGISDKPSQLQRRRLIQAGYTENDGLEDMGRDDLSYLLRFVFRPDSVPTFDSESIGHSEHTFQHLDLHSRNLEMVPIFLYKHADWIVSLDLSGNPMSDLPLDFVQLCSSLRTLRLSNLALKRIPQSVRHSETLTHLDVSNNRIVELAHVSLDLIPELMSLKVQNNRLFDLPSYFSSISTLRNLNISNNRFEEFPKVICDVPSLVDLDVSFNSITELPAEIANLINLERFILAGNELEKLPDSMSELVSLRTIDLRRNKVQDVSSLLGLPRLQNIQAESNNIKSFEATLGPQLTQVELGRNPLSKVRIAALTTCDLTSLDLSSTNMTRLEEGLFPQLPALVKLTLDGNQLVVLPDTLGDLKRLEMLSCSNNLLATLPESIGDLKALKELLVHNNNLKTLPQTLWLCESLAHINLSSNLLESFPAVPDIRTDASVGDAAAAAGTSAVIAARKGSTSSSLTHRSNTGGANGNINLSTPSEVFVAPLSLSLQKLRLGDNRLGDDVFSVLSELTSLEVLNLSFNEIFEIPDFSLQTLTKLRELYISGNQLSTIPSDDLVVLQELRILHLNCNKLTTLPTELGKLKKLANLDVGNNVLKYNIANWHYDWNWNMNPELRYLNLSGNTRLEIKTKLSDMGFTRKSNISDFSRLTSLRMLGLMDVTMPLHSNATPDESDNRRVRTSLSQVNGMAYGIADALGKHDNLSVIDLVIPTFRKDEGECIFGLFDGRGHGAHVGSRIAHHLAEWSGHRLSWEFQKHQNEMTAEPVSVPDALRRAFLRLQKDYADALINDGSRKLSEAHAEAAADVTRSSAPAIAAASNKHDWRAGASAILAYVVDHTLYIANAGDALAVMSRNGGTAHLISNKHEPFDRAEIERIRSAEGWVSLRGYVNDMLDVSRSFGYFHLFPIVNAAPAVTTVQLTDSDEFVIIANRTLWQYVSYQTAVDIARTQRNDPMIAAQKLRDFAISYGAEESIMVMVISVGDLFYRSDQRNGGGLNFASYKNSDAIQKAGRRFREELPGDRTLARLDREVAPPIGQVALVFTDIKNSTSLWETNNGMQTAMRLHNYLLRRQLRTIGGYEVKTEGDAFMVSFPSVSAALLWCFTVQQQLLQEDWPREILDSEDGKEVYDQSGELIHRGLSVRMGIHWGRPVCEADPITRRMDYFGPMVNRAARISGAADGGQILASKDVIKELQGLLGTFDESSTAGGAGGEGENLEKTEEELDEDAFRLLNPNVSRDVVLLRRMGFGLSQLGERRLKGLETPEMLWLVYPKQLAGRLEQAKTDDAPDAPTAQVYEPTVQLLDIEDVKQVGMLCLRLEYLSNSTVCPGIFAAKDEADRSQPSTPLDDNGRNPIDGHGTAVPLLSHQARRKGVEAMLTMHPELLIYSIRDDATDEELAGILDQLTTRIQNAVSSLMLNMLRDKTANGTKELGVDPGVLELLMGLLSQPPPRASTSALSLPSPRTSPRNRLLELVP

Target ORF information:

RefSeq Version	XM_011389967.1
Organism	Ustilago maydis 521
Definition	Ustilago maydis 521 Adenylate cyclase (UMAG_05232), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011389967.1

ORF Insert Sequence:

ATGCTCTTTA CCATGCAATT CACCACTCGA TCCACCGTGG CCTCGCCCGA GCAGCAGCAC 
CAACACCAAC AGCGCAGTAT CTCGTACTCG GATATCGAAC TTGGTCTTGA GCGCATCAGC 
TCTCGCGACA GCAATGGCAG CTCCAACTTT ACGCACAGAG CCTATCCTCC ACCGCTATCT 
CAGCAATATG ACGACACATC CACCAACTCC TTTCATTCAT CGCAGCCAGA CATCACAGCC 
TCTTCTTCAA CTTTGTCGTC TCGCCTCGTG AGCGCCAACT ACTCCCGTCC TCGGTTCGAG 
CATGCGCACA CTCAACCACC CACACCCGAC CAAGACCGCT CCAGCTCCGG CTCCGGCTCC 
GGCTCCGGCT CTCGCTCCTA CTTTCCCGCC AACTCGCATT CCGACTCTTT GCCCGGTCCC 
AGCACTCATA GCATCAGCCC TTCCTTTGAC GAGGACGAAC TCCGTCAGAT CATGTCGCAC 
ATTCCAGCTA ACCAAGCCAC GTCCAGCTCG GATGGCGATG TAGGCAAGGC CGTCCAGTCT 
GCCAACCATC AGGACATCAG TCCGTTCCTC TTCCAGAGCG AGAACGCAGC TCCCTTTTCC 
TCCTCCCACT CCAACCGTAC CAGTGTCAAT CCATCCGCAG CCTCGACCGC CAGCCCGTCG 
ACTTCGGCGG CAACCAGGAC ACGTCCGCGC GGGGGCACCA ACGCCTCGCA GTACAACACC 
CTAGACACTA GCTTCGGTAG CATCGATCGG CCTGGCCTTT CTTCTTCCCG CTCTCAGTAT 
TCATTGCGCC CACAGACCCC ACCCTCGGCG TCCACTTCCA CTAGCACTCT CAACGGCTCC 
AAGGACACGC ACGCTTCTGC CGTCAAGAAG ACGCGCAATC CGTTCGGCTT TCTCAAAAAG 
AAGTCCTCGG CCCACTCAAA TGCTTCCTCG AATCACCCCA CGCGCCACGA CATCGGCTCG 
GTCTCTTCGC TCTCGTCGCG CTACGGTCCC AACGCGGCGG CCAACGTCAA TCCGATGCGC 
CCACCTGCCT GGCTCGATAA TCACTGCACA CTTGCCAACT CCAACTCGCC TTCTTCGGCT 
TCGCTGCGCT CTCACTACCA CCAGCCTCCT GCCTCTTCGA ATCCCCCTCC TTGGCAGAAC 
CCCCTCGTGT CTCGTGCAGA CTCGACTCCT TCCGCCATGA GTCTCGAAGA TGAGGTCGAG 
GCTGAGCATC ATCTCAAGAA GGACCCTCGC AAGCGCATCA AAGGCGTTCG CCATCACCTT 
GCAAAGACCA CCAAGCCCGG CGAGGACGCC GACTCGGCAC GCGACCCTGC CTTTGCAGCC 
CAGAGCCAGT CCATCGAACA AGAGGTCGAA CTCTCGTTGG ACATGAACTT TGACCAGCTC 
GATGACTTTG TCGACACCAA CGCAGCGCGC CAGCGGCTCC AGGGCTCGAT CACTGAGTCT 
GCCAGTCCTT CCGAGCATCG CTCACCAAAC GGAAGCGAAG CCGGCGTGTA TCGCTCGCCA 
TCCCCTTCCC AGACTCCCAT TGCCGAACGC CAGACTTCGG TCACTTCTAC CGTCGAATCT 
CCCTCGCACG CCTCGGAAGC CTCGCTCGCA CCCTCGGGCT CTCTGCGAAC CCCGAGTCGC 
ACTACTGCGT CTACCTCTAC CTCGTCCGCT TCCACCGTAC TCAGCGACCG TCTTCCTTCG 
CAGGTCAACA TGCTTCCTCG CAATAGCGTC CCCAGGCTCA GCCTAGCCGA GATGCAAAAC 
TACCAGTCGC TCCGTAAACT CTCGAACAAC CTCATCGACA TGTCCCAGAC GCAAAACCCT 
TCCGCTATGG GCGCATCCTA CCGCCGCGGA TCCATCGCAG CCGCCCAAGC ACCTGTCGAC 
GCGCCTCAGC TAGGTGTCGC TCCTCGTACT GACTCTGAGC TCTCCGATCG CAAGGACAGC 
GTAGTCTCTA CCCACAGCAT GCGCTCCAAC CATAGCGGCA TCTCGCCCAA GACCTCATAC 
GCCAACCTGC CCAGTGTCAT TCAGGAGCGT CAGAAGCCGG CTACTGCACT TCCCTCCGCC 
GCGAATTGGA CATCATCCAT CACCAGAGAC AAAACGGCCA ATGGACATGC TGACCACGCC 
TACCAATTTC CGCCCGCAAC TGAGTATCAG TCCAACTTGC TGCTCAGCGT GCGCAAGTCG 
AGTGCCAGCT CGGGGCAGGA GCCTAGCTCC AGCTGGATGG CTCCCGACAG TTGGGCCGTA 
CAACCGGACA AGATGCGCGA CTATCTTCGC GACGATAATG TCGGCGAGGA GGAAGACGAC 
GACGACGACC AGCACCAAGC CAGAGCCGCT TTGGCCACCG ATGGCAAGAG GCGCGGTTCC 
AGCTCCGGTA TCAGCTCGAC GCATGCATCG AGCATGTTCC GCACCTCATC CACCGACCCT 
TTCAAGAAGA CTGCCTCGCT TGCTGGCTCA CGTCGCGGCA CGGACGACTC GGTCGATCCG 
CTCACCGCTT TGCCTCCATT GCCAGGCTCC AAGTCGGTGG ACGAGGCAGC AGCCAACAAG 
GTCGATGTAC TCCAGCAGAC CAATAATCTC GCTCAATCGG CTCTGGTGCA ACAGCAGTCT 
CAATCCCAGA ACCACCACCA GCCCAGCCCC AACGTCCGTC CCACCTCGAG AGGCGGTGCT 
GGTGCCCACA TGTTTGCCTC CGCCGGCGCT TCCGCTGCAG CAGCTGCTGC GGGCAAGCTT 
GGTTTACATC GTCCATCTAA ACATCGTATG AACGCGCGAC CCAATACCGC AGGCTCCGTT 
GGTGCGACCC GACCTTCCAC CACCACGCTC GGCTCGACCC TCTCGGCAGA GGACGACACA 
AGCATCAACG GCTCCATCCG ACGCGACGGC CATCCGCTCA AGCGCTCCGC AACTGCCAAC 
ACGAACAACG CCACGGGAAC TTTGCCTCGC AACCACTTCA TCCGCGTCTA CAAGACGGAC 
GGCACCTTTG CAACGCTCTC GTGCTCACTC GTCTCGACGG CAAACGAAGT ACAGACAATC 
CTCGCGCGCA AGAGCCTCAC CACCGAATCG GCCGCATACA GGCTTTTTGT GCGGGACAAG 
GGCTCCGAGA GACCGCTGGG GATCTCGGAC AAGCCTTCTC AGCTCCAGCG TCGTCGTCTC 
ATTCAGGCAG GCTACACCGA GAACGATGGC TTGGAAGATA TGGGCAGAGA CGATTTGTCA 
TACCTGCTTC GCTTCGTCTT TCGCCCCGAC AGTGTTCCCA CCTTCGACTC GGAATCCATC 
GGCCACAGCG AGCACACATT CCAGCATCTC GATCTGCACA GCCGCAATCT CGAAATGGTG 
CCCATCTTTC TGTATAAGCA TGCCGACTGG ATTGTTAGCC TCGATCTGTC AGGCAATCCC 
ATGTCTGATC TTCCGCTCGA CTTTGTACAG CTCTGTTCAA GCCTGCGAAC TCTGCGACTC 
TCAAACTTGG CGCTCAAGCG CATCCCGCAA AGTGTCCGGC ACAGCGAAAC CTTGACGCAC 
CTCGACGTAT CCAACAATCG AATTGTCGAG CTCGCGCATG TCAGTCTCGA TCTTATTCCG 
GAGCTCATGT CGCTCAAGGT GCAAAACAAT CGTCTCTTTG ACTTGCCCTC ATACTTTTCC 
AGCATCAGCA CGCTGAGGAA CCTCAACATC TCTAACAACC GCTTTGAAGA ATTCCCCAAG 
GTCATCTGCG ACGTCCCTTC GCTGGTCGAT CTTGATGTGT CATTCAATTC GATCACTGAA 
TTGCCCGCCG AAATCGCCAA CCTCATCAAC TTAGAGCGCT TCATCCTAGC CGGCAACGAG 
CTGGAAAAGC TGCCGGACAG CATGAGCGAG CTCGTCAGCT TGCGCACCAT CGACCTGCGT 
CGTAACAAGG TGCAGGACGT TTCGTCGCTG CTTGGTCTTC CACGCCTTCA AAACATCCAG 
GCCGAGAGCA ACAACATCAA GTCGTTTGAA GCTACACTGG GTCCGCAACT CACTCAGGTC 
GAGCTCGGTC GCAACCCACT CAGCAAGGTG CGCATCGCTG CTCTCACCAC GTGCGATCTC 
ACCTCGCTCG ACCTGTCGTC GACCAACATG ACGCGGCTCG AGGAAGGTCT CTTCCCGCAG 
CTGCCTGCGC TCGTCAAGCT GACGCTCGAT GGCAATCAGC TCGTTGTGCT GCCCGACACG 
CTCGGAGACT TGAAGCGACT CGAGATGCTA TCATGCAGCA ACAATCTGCT TGCTACCCTC 
CCCGAGTCGA TCGGTGATCT CAAGGCGCTC AAGGAGCTGC TTGTGCACAA CAACAACCTC 
AAAACGCTTC CACAGACCTT GTGGCTCTGC GAGAGCCTCG CACACATCAA TCTCAGCTCC 
AACTTGCTCG AATCGTTCCC AGCAGTGCCT GACATTCGCA CCGATGCTTC CGTTGGAGAT 
GCGGCTGCAG CTGCCGGCAC TTCGGCCGTT ATCGCTGCAC GAAAGGGATC TACAAGCTCG 
TCGCTGACGC ACAGGTCTAA CACTGGTGGC GCCAATGGGA ACATCAATCT CTCTACGCCC 
TCCGAGGTCT TTGTGGCGCC GCTCTCGCTC AGCCTGCAGA AGCTGCGTCT GGGAGACAAT 
CGTCTAGGCG ATGACGTTTT CAGTGTCCTG TCCGAGCTCA CCTCGCTCGA GGTGCTCAAC 
CTCAGCTTCA ACGAGATCTT TGAGATTCCC GATTTTAGTC TCCAGACGCT CACCAAGCTG 
CGTGAGCTAT ACATCAGTGG TAATCAGCTT AGCACGATTC CTTCCGACGA TCTTGTGGTG 
CTACAAGAGC TGCGCATCCT CCACCTCAAC TGCAACAAGC TCACTACTCT CCCCACCGAA 
CTTGGCAAGC TCAAGAAGCT CGCCAACCTG GATGTCGGTA ATAACGTGCT CAAGTACAAC 
ATTGCCAATT GGCATTACGA CTGGAACTGG AACATGAACC CGGAGCTACG CTACCTCAAC 
CTTTCCGGCA ACACGCGTCT CGAGATCAAG ACCAAGCTGA GCGATATGGG CTTCACGCGC 
AAGTCCAACA TCTCGGACTT CAGCCGCTTA ACCAGCTTGC GCATGCTGGG TCTCATGGAT 
GTCACAATGC CATTGCATTC AAATGCCACG CCGGATGAGT CGGACAACCG CCGTGTGCGA 
ACCTCGCTCT CGCAGGTCAA CGGAATGGCT TACGGAATCG CCGACGCACT CGGCAAGCAC 
GACAACTTGA GCGTCATCGA TCTTGTGATT CCAACATTCC GTAAGGACGA AGGAGAGTGC 
ATTTTTGGTC TATTTGACGG TAGGGGCCAC GGTGCACATG TTGGCAGCCG TATCGCGCAT 
CACCTGGCCG AATGGAGTGG ACATCGTCTA TCGTGGGAGT TCCAGAAGCA CCAGAATGAA 
ATGACCGCCG AACCTGTTTC GGTTCCGGAT GCGCTGCGCC GCGCCTTCCT ACGCCTGCAG 
AAGGACTACG CCGACGCGCT CATCAACGAT GGCAGCCGCA AGCTCTCTGA AGCACACGCA 
GAGGCGGCTG CGGATGTGAC GCGAAGCTCT GCGCCTGCGA TTGCGGCCGC CTCCAACAAG 
CATGACTGGC GCGCCGGCGC TTCCGCGATT TTAGCGTACG TGGTCGACCA CACGCTCTAC 
ATTGCTAATG CGGGTGATGC TCTGGCGGTC ATGTCGCGCA ACGGTGGCAC AGCACATCTG 
ATCAGTAACA AGCACGAGCC GTTTGACCGT GCCGAGATCG AGAGGATTCG ATCGGCGGAA 
GGATGGGTCT CGCTGCGTGG CTATGTCAAC GACATGCTCG ATGTCTCGCG TTCGTTTGGC 
TACTTCCATC TGTTCCCGAT TGTCAATGCT GCACCTGCGG TGACGACGGT GCAGTTGACT 
GACTCGGATG AATTTGTGAT TATCGCCAAC CGCACGCTGT GGCAGTATGT GTCGTACCAG 
ACCGCGGTCG ACATTGCACG TACACAGCGA AACGATCCTA TGATTGCAGC GCAGAAGCTG 
CGAGATTTTG CCATCAGCTA CGGTGCCGAG GAGAGCATCA TGGTCATGGT GATCTCGGTG 
GGTGATTTGT TCTATCGTTC AGATCAGCGT AACGGCGGTG GACTCAACTT TGCGTCGTAC 
AAGAACAGCG ACGCCATCCA AAAGGCAGGA CGTCGATTCC GCGAGGAGCT GCCTGGCGAT 
CGCACGCTGG CGAGACTGGA CCGTGAAGTG GCACCTCCCA TCGGACAGGT GGCGCTGGTG 
TTTACGGATA TCAAGAACTC GACGTCGCTG TGGGAGACCA ACAATGGCAT GCAGACGGCG 
ATGCGATTGC ACAACTACCT GTTGCGACGA CAGTTGCGCA CTATTGGGGG TTATGAGGTC 
AAGACGGAAG GCGATGCATT TATGGTTTCA TTCCCCTCGG TCAGTGCAGC TCTGCTGTGG 
TGTTTTACAG TACAGCAACA GCTGCTGCAA GAGGACTGGC CGCGCGAGAT CTTGGACAGC 
GAGGACGGCA AGGAGGTGTA CGATCAGTCG GGCGAACTCA TTCATCGAGG TCTGTCGGTG 
CGCATGGGCA TTCACTGGGG ACGGCCAGTG TGTGAGGCGG ATCCGATCAC GAGACGAATG 
GACTACTTTG GACCGATGGT GAACCGTGCT GCTCGAATCA GCGGTGCAGC CGATGGCGGT 
CAGATCTTGG CGAGCAAGGA CGTAATCAAG GAGCTGCAGG GCCTATTGGG TACGTTTGAC 
GAGTCGTCGA CAGCCGGAGG TGCGGGCGGA GAAGGTGAAA ATCTAGAGAA GACCGAGGAG 
GAGCTAGACG AGGATGCGTT CCGACTGCTC AACCCGAACG TCAGCCGCGA TGTGGTGCTG 
CTACGTAGGA TGGGTTTTGG TCTATCGCAG CTGGGAGAGC GCAGACTCAA GGGTTTGGAG 
ACGCCCGAGA TGCTGTGGCT TGTCTATCCG AAGCAGTTAG CAGGACGTCT GGAGCAGGCC 
AAGACCGACG ATGCACCTGA CGCACCCACG GCGCAAGTGT ATGAGCCGAC AGTGCAGCTG 
CTAGATATCG AGGATGTAAA GCAAGTGGGC ATGCTCTGCT TGCGACTCGA GTATCTGTCC 
AACTCTACGG TCTGTCCTGG CATCTTTGCT GCCAAGGATG AAGCGGATCG ATCGCAACCT 
TCGACGCCTC TGGACGATAA CGGACGGAAT CCGATCGACG GCCACGGAAC GGCGGTGCCC 
CTGCTGTCAC ACCAGGCGCG ACGCAAAGGG GTTGAGGCGA TGTTGACGAT GCATCCCGAG 
CTGCTCATCT ACAGCATCCG AGACGATGCG ACGGATGAAG AGTTGGCGGG CATCTTGGAT 
CAGCTGACGA CGCGGATCCA GAACGCCGTG TCTTCGCTCA TGCTGAATAT GTTGCGGGAC 
AAGACGGCCA ACGGTACCAA GGAACTTGGC GTGGATCCTG GGGTGCTCGA GTTGTTGATG 
GGCTTGCTTT CACAGCCGCC TCCGCGAGCG TCAACGTCGG CGTTGAGTTT GCCCAGCCCA 
CGGACGAGCC CTCGCAACAG GCTGTTGGAG CTGGTTCCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis.
Nature444(7115)97-101(2006 Nov)
Kämper J,Kahmann R,Bölker M,Ma LJ,Brefort T,Saville BJ,Banuett F,Kronstad JW,Gold SE,Müller O,Perlin MH,Wösten HA,de Vries R,Ruiz-Herrera J,Reynaga-Peña CG,Snetselaar K,McCann M,Pérez-Martín J,Feldbrügge M,Basse CW,Steinberg G,Ibeas JI,Holloman W,Guzman P,Farman M,Stajich JE,Sentandreu R,González-Prieto JM,Kennell JC,Molina L,Schirawski J,Mendoza-Mendoza A,Greilinger D,Münch K,Rössel N,Scherer M,Vranes M,Ladendorf O,Vincon V,Fuchs U,Sandrock B,Meng S,Ho EC,Cahill MJ,Boyce KJ,Klose J,Klosterman SJ,Deelstra HJ,Ortiz-Castellanos L,Li W,Sanchez-Alonso P,Schreier PH,Häuser-Hahn I,Vaupel M,Koopmann E,Friedrich G,Voss H,Schlüter T,Margolis J,Platt D,Swimmer C,Gnirke A,Chen F,Vysotskaia V,Mannhaupt G,Güldener U,Münsterkötter M,Haase D,Oesterheld M,Mewes HW,Mauceli EW,DeCaprio D,Wade CM,Butler J,Young S,Jaffe DB,Calvo S,Nusbaum C,Galagan J,Birren BW

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UMAG_05232 cDNA ORF clone, Ustilago maydis 521

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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