TGME49_250760 cDNA ORF clone, Toxoplasma gondii ME49

Gene
Clones
gRNAs

Summary

Gene Symbol	TGME49_250760
Entrez Gene ID	7897194
Full Name	pentatricopeptide repeat domain-containing protein
Synonyms	TGME49_050760
Gene Type	protein-coding
Organism	Toxoplasma gondii ME49

mRNA and Protein(s)

mRNA	Protein	Name
XM_002367317.2	XP_002367358.1	pentatricopeptide repeat domain-containing protein

Pathways from BioSystems
Gene Ontology

The following TGME49_250760 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TGME49_250760 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTi02489	XM_002367317.2 Latest version!	Toxoplasma gondii ME49 pentatricopeptide repeat domain-containing protein mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TGME49_250760 ( XM_002367317.2 ) cDNA ORF clone, Toxoplasma gondii ME49 -> XP_002367358.1 Toxoplasma gondii ME49 pentatricopeptide repeat domain-containing protein mRNA.

Price & Availability↑

CloneID	OTi02489
Clone ID Related Accession (Same CDS sequence)	XM_002367317.2
Accession Version	XM_002367317.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1476806400000
Organism	Toxoplasma gondii ME49
Product	pentatricopeptide repeat domain-containing protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_031480). On Oct 20, 2016 this sequence version replaced XM_002367317.1.

ATGCCGACGT CTCTCAGCGG AGGCGTCTTC TGGTCCAAAC TGGTCCAGCG GACGCCGGCA 
GCCGCAGCGT CGACTGCACC TGCTGCCTCT TCAGAAGCCT CACCTGCCCT GAAGCCGGCG 
TTTCTGCCCG CGGACCAGGC GGGCAGATCT ACCAGAGCGT CCCAAAGCGG TGCTCTGACT 
GCTCCGCACT CTTCGCGGAA GGGAGCTTCG TTGACGGCGT CGACCGGCGG CGGCCGCGAC 
CAGTCGTTCT TTTGGCCAGT CTTCGGTGGC TCCCCGCTCC AGATCTCTGG CGTCCTCATC 
CCGTACTACA TCTGCCCTCT CCTTCCGAAC TTCCCGAACG CAACTTTCTA CAGCTACGAC 
GGCTGCTTCG AGGCCGGCGC AGAGGGTCGT TCGAAGGAAC GAACTCTCGC GAACTCCCCC 
CGCCAAAGCC TGGGCTTCGA GGACGACGCC TGCGGTTCCA TCGCCTCGAG TCACTTCTGG 
GCTCCAGAGA CGGTGCATCC GCTCGGACTC GCGGACGTCA CGCCGCGCAG AGCAGAAGTC 
TTCCAGCCAG GTTGCCCGAA CGGCCAGCTC TTCGGGAGCG AGGCGGACCT CAGCAGGTTT 
GCTCCCGTGT ACCACCAGGC GCCCCTTGAA GGCTGCGCGC GCGCGCCGGT CTTTGGAGTT 
CACGCCGGAC AGCCGGAAGC TGCGCTGGAC AGCTCTGCGC ATTCGGCTGC GCTCCTCGCG 
AACGCCGCAC CCGCAGCCAG AGAGCCCGAG GCTGTCGGCC CCAAGCCGGA GACGAAACGC 
CGTCTGTCTC CGTCGTTCCA CGGCCGACGA GGAAGTTTGA CGTACTTCTT CCACCACTGC 
GTACCACGCA GAGTCCAGCA TCTGCTACAG TGGTCGATTC CGAACTTCCT CGTCCTCTTC 
ATCTTCGCAT GTGTACTGCC CGCCTGTCTG AAGTACCGAG CCCCCATTGC AATCGACTTC 
GCGATCGTCG GCCTGTTCCT CTCCAGCGTC TGGGGCTTCA TCGCCATCAA GTCTGACGCT 
GCGTCCAAGA TCGCCAGACG CTCGCAAGAG AAGGATGGGC CGGGGGAGAA GGAGCTTAAG 
CTCCAGGAGA AGAAGTGCAG GCTTCGCCCG TGTGCGACCC CGGCGGTGCG TGTCGAGACA 
GTCCGGAAGG TGGGTGAGAA AACGGAGAGA AGGAACTCTC CGAAGAAGGA CGTCGCGACC 
CTTGAAGCGA CTCACGAGAC CCGCACTGAG TGCCTGCTAG TTAAGCCAGA AAAGCAGACG 
ACAGCGTATC AAGAGGCTTC GGACCAGCCC GCCGTCGATG ACGACGAAAG CAAGCAGGTG 
CGTTCGGCAT CAGACGATCT TGCTCTCTCA CCCACGACGA ATTCTGTTCT CGACAAAGAC 
GAACAGTCCC CCGAGGAAGA TGGACAGACC CTCTCCGCTC CTTGTGCCTC CACCCTCGAG 
ACGTCGGAGG CTGCAGTGGA AGAGCCTGCC AGTGTCGAAG ACGAGGAACC ATCGCCTGGA 
TGCGACAGTG CCGATCCGGA GATCAGGGAG CCAGGAAGCA AGCAACCAGG GGGTTCTCTA 
CCTCAGGACG GACAGACAGA GCGCGCTGTA GAGCAGAAGA TTGAAACGTT TTCGAGTGCT 
TTGGTAGAAC GGGAGAGAGA AGATGTGACA GTCACGAGCT GTGCGGGAGG AGAAACTGTT 
GCCAACGCTT CGCAGTGCCC TGAGGACCAT TCGGACCTCT CTTTGTTGAA CAAGGAGGAA 
GACGCAGCGA ACATCGCGAC CCGTCTAGCA GAAACCCTGC AGTCACTGCC AGCAATCTCT 
GAGTGCACGG AGAGTCAGAC GGAAGACCAG GACCCCGAGA GCTCCGGGGA CGCTTCGTTC 
GTGGTCGAGG CGGAGTCTGC AGCGCGTGTG GCTCCGGAGG CTTCTGCCGC CTCCCAAGTT 
GTATTCGCGG GTGAGGAGGT TGTCGATTCC CTGGATTCCG AGTTGGCCGC GCTTGCAGAG 
ATATCTGCGG AAGTGGAAGA GTGTGGAGAA AACGCGGAAG AGGGCGAAGC AGAGGAATCG 
TGTGTCCTGG AGACCGATCC GTCAGCAGAA GCGCGAGAAA AGGAGCTCAT AAGCGACTTG 
GCGTCCCCGT TCTGGGCGTC GCCCCCGGCG TTGACTGCGC GTCAGCCCCG GTGTACTGCA 
AAGAAGGCGG TTTCCTTCTC TCCGTGGAAT GAGGAGAGGC CGACGGAGGC AGGTGAGGCG 
ACAGAGGCCG CGCGCGAGGT CGAGCTAGGC CTGGCAGCCC AAAGTGGGTC CGAGCTCGGG 
AAGAGTCCCG AGGCGGAACG GGCGGGAGAG GAAGCGGAGC CAGACTTTGG AGACGGTCTG 
CAGCGGTCGC ACCGAGCGGC GCTGTCTCCA GCGCTGGCTC TTTTGTACGA CGAGGACGTG 
TCAGTGTCGG AGGAGATTTT ATCCTGTCAG TCTTTGACGG ACGTCTCGCT GATGTCTCGT 
TCGCCCTCGA CATGCGATGG AAACGAACGG GCGCTGTCGC GGCCGACAGC TTCTCTGGAT 
TTGCGTGCGC GCGGGGAGAC CTTTATCGCA GGCCTCCCAG AGACTCGAGA CGAGGTCGAC 
GTGGAGCGCG ACTCCTCCTC CTCGAAGAGG ATTGCAACTC CAATGCACAT TGGCGTGGAC 
GTCGACGAGG GCGGCATCGC GGGAAACGTG AAGTCGTTCT GCAGTCACGC GTCTCTCGCT 
TCTTCCTTCC TGTTGGAGAG CGAGGACGAC CGGAGTGACC AGGCCTCGGG GGTGAATGGC 
TGCTTGGAGG CGACGTTCGA GAGCGAAGAG CCTCCGCTCC ATACCACCTT CATGGCTGAG 
CAGCTCCGTC TGCCGCTCGC GGCGCTGACG GAGGGCTCAG AGCTCGTCGC GCAGGAACCC 
GAGGTCGAGA AGGTTCTCTG GAAAGTCGAA GACACGCAGA ACACGACAGC GGAGGATTGT 
GCTTTCTCTT CGGTCTCCTC GAACGCGAGT CTCTTCCGCT CTCAGTCCCC CGAGACGCGC 
ATCGCCGGCG CCCCAGCGCC TGCGCCGGGC GCCCCCAGCC TCTGCCTCCC AGTGGCAGCC 
TTCCCGGGGT TCGCGGGCAC AGAAGCACCA TTCCCCTTTG TCGCTTCTGT CCCGAACGGC 
GTGCGAGACT CGCGTCTCGC GTTCGGTGTG GGGTCCATCG AGCGTTTGCT CCGCGTCGAG 
GATGAGACTA AGCAGTCTGG CCCTCCGGGG CTCCGCGGCT TCCCCGCCGG CCACTCTGAG 
ACCGCGAGCG AGGCGAACTT CCCGTTCGGA AGGTTCATCT CAGAGTCCCT CTCGTTCTCC 
CCGACGGTTT CCTTCGGATC GGGGCCCCTC GGGTTCCAGT CCGCCCCCGC GTGCGGCCTG 
AACATCGAAG AGCTCCTCGA CCTCGACAGT TTGGATGCGC AGGACCGGGA TGATGTGCGG 
CAACTCAAGA AGCAACTGGG TCACGAGTCC GACGACGCCG CCATCCCCCA GGCTGCGACA 
CCCTTCGTCC TCCCAGACGA CCCTGCGGGC TCTGCCTCCC GCGCAGGCGC CCCGGCCTTC 
CCGCCCCCTC CACCCCTTAG GGCCCCACGC GCCACTGCGG CTCCGCCCGA AAACTGCCGG 
GCCTCCTCTG GGCCCCAGAG GCCCCACGCG GCGTCACTGC GCGCCCCGCC GCCGCCTGTC 
TCGCCCCCGC AGGACGAGGA GGCCCCGCCG CCGGCTACGG TGACCTCTCA ACCGAACAGC 
TCGAAGCAGC GGATCGCGCT GCAGCACTAC ATGGCGCTCG TTCGCGAGTG CCACCGCACC 
AAGGACAGTC GCGGGGCGAT TCGCGTTCTC GGGCGACTCC AAGCTGAAGG TCGCGTTGCT 
GCTGACACCC AGCTCCTCAA TTACGTGCTG ATGGTTTGTG TCTCCGCGAA CGACCGCGTC 
ATGACAGGGC AGTTGTTCAA GTTCATGGAG ACGACGGGGT GCGCAGATTT GGTGACGTAC 
AACACACTGG TGAAGAGCTT CACGGCAAAC GGGGAACTGC ATGCGGCAGA GCAGGTGCTT 
GCTCGAATGG AGGGTCGCGA GAACACGTTG GAGGCGGAGC TGTCGGGTGC ACATCTGGGG 
ATGGGTTTCG AGGGCAAGTC GGAGAACGGG GTTTTGAATA GACCGAAGAT CTCTCCGGAC 
GAGGTGACTT TCAATCTGTT GGTGAACGCG GCGGTGAGCG CAGGCGACCC CGACAAGGCA 
TGGGAGTACA TTGACCGGAT TAAGAAGGCG AATCTGCGTC CGGACAAGTT CACGATTAGT 
TCGATCATCA AGTCGCTGCA GCCGGGACAG AACGAGCAGC ACACGCGGCG CGCGTTCGAG 
TTGATGGATC AGATCGATGC GTGCGAAGAC CTTGTTCTCC TCAGCACCTG CATCGATGCA 
TGCGCGCGGC TGGGTGACCT GGACCGACTC GCCACACTCC TCGCGCGCTT CGAAGACTCC 
GACTTGAAGC CGAACGCGCA TGCGTACGGG ACGCTAATCA AGGCGTACGG GAAGCTCGGC 
AACGTGACCC GTGTCTGGGA GCTTTGGATG GAGCAGCAGC GCAGTGGCAT CGAGGCCTCG 
AACTACACAC TCGGGTGCAT GATCGACTGT CTCGCGAGCA ATGGATTGAT GAACGAAGCC 
GTCGACCTGT TTGGGAAGAG CGTCGCGGCG GGGTCTGCCG ACGCGGTGCT CTTCTCCATT 
CTGGTCAAGG GCCTCGCTCG ATTCCGAAAT CGCGGCCTGG AGGTTGCCCT CGGAGCGTAC 
CGGCGCCTGA AGGAGAGCGA CAACTCGGCC GCGGTGACTC CTGCTTCGAA GCCTGCTGCG 
GGGAGCCGCG CGCCAACCGG AGGCCCGTCT TGTGGGGTGG ACGGTAGCCG TGAGAGTCGG 
AGACGCGGAG GCAGCAAGCG CCCCGTGGGG GGCGAGAAGG GGGACAAAGC CGGGGCTGCG 
CGCGATGGTG ACGGGGAGGA GCATAGCTGC TATCTGAAGG CTCTCAACAC CATTTCCTTT 
AACTCGCTCC TCCACCTCTG CGTGCGCTCC GGACGCCTGG CGGACGCTCT CGAACTCTTC 
AAAGACATGC AGTCCCACCC GCATGCGCAG CCGGATCTCA TCACCTACAG CACAGTCATC 
AAGGGCCTCT GTTGCTCCTC CAAGGAGCAC GCGCTAGACG CCGCCTTGGG CCTGTTCGAG 
CAGATGCAGA AGGACGCGAA GATCTCCCCA GATGCAATTG TGTACAATAC CCTCATCCAG 
GGCGCGGCCA CTCGCAGAAA CGTCACTCTC GTTGAGTCGT TGCTTCTCCA CATGCTCCAA 
AACAAAGTGA AACCCAGCAG CTACACGCTG TGTCAGTGCG TCAAGCTCTA TGGCAAGTGC 
GGTGACCTCG CTCGCGCCCT GGAGCTTGCG CTCGAGCTTC CACGTCGTTT CAACTTCGCG 
ATGGACAGCT ACGTGTACAC GGCGCTGATC GCTGCCTGCA CACACAACCG GGCGCCGTTC 
CTGGCGGCGA CGCTCGCCCT GCAGGCGACG CAGGAGAACC AGGAGTTACC TCCTGCTTTG 
CTTCTGCGTC TGGCGAACCA TCTGCAGCAG CAGCGGGAGC TGGCCGAGAG AGAAGCGATG 
AACTCGAGCT TGCGAGAGTT GCCACTGCTT CAGAACGTGT GTTCGAGCGA GCTGAATGGA 
AAGGTTCTCG GTCCGTACGG GGTCCGAAAA TTGTCCCCGG CGGAGGAGGC GAAGGCGACT 
GTCGACGCCA TCATCAAGTT GCTGAAGCGC GCGGTCGGAA GCACCGGGGC TCCGGGGGCG 
AGGGAGGACC GCGAAGGTTC TGGGGAGGCC CTCTCATTCG CGTCTTTCTC GTCTCCTGGC 
GACAGTTCAC AGAGAGATGG GGGTCGCGGG GCCTTCGCGA CGTCGCGCAC CGCCGCTGGC 
AGCAAGCGGT CGAAGCGTGG TGGCGGCCAG TCCGTGGTTC CCGTTGTGGA CCGAGAGCGC 
GGCGAGAGAG TGGAGAAGGC GACCGCGTTC TCCGGGGTAA ACGGCAAGCG AAAGCCGGGA 
AACAAGGTCC AGGAGACCAG GGCGGCTGCG GACGACGGGG GCCCTCCAGG TCTGCATGCA 
GGAGCGACAA GCCGCGGGTT TGGCGGGCGC CAGGGTGGCG AGAGCGGCCC GAAGACATTT 
GCTGGGCAGC GCAAGCAGCT GGTGAACGCG GGGACGCCGC CTCCACCGCA GACTGCGTTC 
AAAAAGAGCT CGGTGCATGC AAGTCGAGTG GGGGGTTTGC CGCGAGGCAG CGGCGGACTG 
GAGAGCACGA ACTTGTACCC CTTTGAGGAC ATGGGACTGA ATTTGGATTT CGATGGCCCG 
CGCATGCGTG GGAGACACAG CAGCGGGGCG TCGAGCGCCG TCACGACTGC CAGCTTCTCA 
AAAGAAACCG CGAGAAGCGG GCGGAAGGGA CATGCCAACA ACGGGCGAGG GGGTCGTCGA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002367358.1
CDS	1133..7558
Translation	MPTSLSGGVFWSKLVQRTPAAAASTAPAASSEASPALKPAFLPADQAGRSTRASQSGALTAPHSSRKGASLTASTGGGRDQSFFWPVFGGSPLQISGVLIPYYICPLLPNFPNATFYSYDGCFEAGAEGRSKERTLANSPRQSLGFEDDACGSIASSHFWAPETVHPLGLADVTPRRAEVFQPGCPNGQLFGSEADLSRFAPVYHQAPLEGCARAPVFGVHAGQPEAALDSSAHSAALLANAAPAAREPEAVGPKPETKRRLSPSFHGRRGSLTYFFHHCVPRRVQHLLQWSIPNFLVLFIFACVLPACLKYRAPIAIDFAIVGLFLSSVWGFIAIKSDAASKIARRSQEKDGPGEKELKLQEKKCRLRPCATPAVRVETVRKVGEKTERRNSPKKDVATLEATHETRTECLLVKPEKQTTAYQEASDQPAVDDDESKQVRSASDDLALSPTTNSVLDKDEQSPEEDGQTLSAPCASTLETSEAAVEEPASVEDEEPSPGCDSADPEIREPGSKQPGGSLPQDGQTERAVEQKIETFSSALVEREREDVTVTSCAGGETVANASQCPEDHSDLSLLNKEEDAANIATRLAETLQSLPAISECTESQTEDQDPESSGDASFVVEAESAARVAPEASAASQVVFAGEEVVDSLDSELAALAEISAEVEECGENAEEGEAEESCVLETDPSAEAREKELISDLASPFWASPPALTARQPRCTAKKAVSFSPWNEERPTEAGEATEAAREVELGLAAQSGSELGKSPEAERAGEEAEPDFGDGLQRSHRAALSPALALLYDEDVSVSEEILSCQSLTDVSLMSRSPSTCDGNERALSRPTASLDLRARGETFIAGLPETRDEVDVERDSSSSKRIATPMHIGVDVDEGGIAGNVKSFCSHASLASSFLLESEDDRSDQASGVNGCLEATFESEEPPLHTTFMAEQLRLPLAALTEGSELVAQEPEVEKVLWKVEDTQNTTAEDCAFSSVSSNASLFRSQSPETRIAGAPAPAPGAPSLCLPVAAFPGFAGTEAPFPFVASVPNGVRDSRLAFGVGSIERLLRVEDETKQSGPPGLRGFPAGHSETASEANFPFGRFISESLSFSPTVSFGSGPLGFQSAPACGLNIEELLDLDSLDAQDRDDVRQLKKQLGHESDDAAIPQAATPFVLPDDPAGSASRAGAPAFPPPPPLRAPRATAAPPENCRASSGPQRPHAASLRAPPPPVSPPQDEEAPPPATVTSQPNSSKQRIALQHYMALVRECHRTKDSRGAIRVLGRLQAEGRVAADTQLLNYVLMVCVSANDRVMTGQLFKFMETTGCADLVTYNTLVKSFTANGELHAAEQVLARMEGRENTLEAELSGAHLGMGFEGKSENGVLNRPKISPDEVTFNLLVNAAVSAGDPDKAWEYIDRIKKANLRPDKFTISSIIKSLQPGQNEQHTRRAFELMDQIDACEDLVLLSTCIDACARLGDLDRLATLLARFEDSDLKPNAHAYGTLIKAYGKLGNVTRVWELWMEQQRSGIEASNYTLGCMIDCLASNGLMNEAVDLFGKSVAAGSADAVLFSILVKGLARFRNRGLEVALGAYRRLKESDNSAAVTPASKPAAGSRAPTGGPSCGVDGSRESRRRGGSKRPVGGEKGDKAGAARDGDGEEHSCYLKALNTISFNSLLHLCVRSGRLADALELFKDMQSHPHAQPDLITYSTVIKGLCCSSKEHALDAALGLFEQMQKDAKISPDAIVYNTLIQGAATRRNVTLVESLLLHMLQNKVKPSSYTLCQCVKLYGKCGDLARALELALELPRRFNFAMDSYVYTALIAACTHNRAPFLAATLALQATQENQELPPALLLRLANHLQQQRELAEREAMNSSLRELPLLQNVCSSELNGKVLGPYGVRKLSPAEEAKATVDAIIKLLKRAVGSTGAPGAREDREGSGEALSFASFSSPGDSSQRDGGRGAFATSRTAAGSKRSKRGGGQSVVPVVDRERGERVEKATAFSGVNGKRKPGNKVQETRAAADDGGPPGLHAGATSRGFGGRQGGESGPKTFAGQRKQLVNAGTPPPPQTAFKKSSVHASRVGGLPRGSGGLESTNLYPFEDMGLNLDFDGPRMRGRHSSGASSAVTTASFSKETARSGRKGHANNGRGGRRS

Target ORF information:

RefSeq Version	XM_002367317.2
Organism	Toxoplasma gondii ME49
Definition	Toxoplasma gondii ME49 pentatricopeptide repeat domain-containing protein mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002367317.2

ORF Insert Sequence:

ATGCCGACGT CTCTCAGCGG AGGCGTCTTC TGGTCCAAAC TGGTCCAGCG GACGCCGGCA 
GCCGCAGCGT CGACTGCACC TGCTGCCTCT TCAGAAGCCT CACCTGCCCT GAAGCCGGCG 
TTTCTGCCCG CGGACCAGGC GGGCAGATCT ACCAGAGCGT CCCAAAGCGG TGCTCTGACT 
GCTCCGCACT CTTCGCGGAA GGGAGCTTCG TTGACGGCGT CGACCGGCGG CGGCCGCGAC 
CAGTCGTTCT TTTGGCCAGT CTTCGGTGGC TCCCCGCTCC AGATCTCTGG CGTCCTCATC 
CCGTACTACA TCTGCCCTCT CCTTCCGAAC TTCCCGAACG CAACTTTCTA CAGCTACGAC 
GGCTGCTTCG AGGCCGGCGC AGAGGGTCGT TCGAAGGAAC GAACTCTCGC GAACTCCCCC 
CGCCAAAGCC TGGGCTTCGA GGACGACGCC TGCGGTTCCA TCGCCTCGAG TCACTTCTGG 
GCTCCAGAGA CGGTGCATCC GCTCGGACTC GCGGACGTCA CGCCGCGCAG AGCAGAAGTC 
TTCCAGCCAG GTTGCCCGAA CGGCCAGCTC TTCGGGAGCG AGGCGGACCT CAGCAGGTTT 
GCTCCCGTGT ACCACCAGGC GCCCCTTGAA GGCTGCGCGC GCGCGCCGGT CTTTGGAGTT 
CACGCCGGAC AGCCGGAAGC TGCGCTGGAC AGCTCTGCGC ATTCGGCTGC GCTCCTCGCG 
AACGCCGCAC CCGCAGCCAG AGAGCCCGAG GCTGTCGGCC CCAAGCCGGA GACGAAACGC 
CGTCTGTCTC CGTCGTTCCA CGGCCGACGA GGAAGTTTGA CGTACTTCTT CCACCACTGC 
GTACCACGCA GAGTCCAGCA TCTGCTACAG TGGTCGATTC CGAACTTCCT CGTCCTCTTC 
ATCTTCGCAT GTGTACTGCC CGCCTGTCTG AAGTACCGAG CCCCCATTGC AATCGACTTC 
GCGATCGTCG GCCTGTTCCT CTCCAGCGTC TGGGGCTTCA TCGCCATCAA GTCTGACGCT 
GCGTCCAAGA TCGCCAGACG CTCGCAAGAG AAGGATGGGC CGGGGGAGAA GGAGCTTAAG 
CTCCAGGAGA AGAAGTGCAG GCTTCGCCCG TGTGCGACCC CGGCGGTGCG TGTCGAGACA 
GTCCGGAAGG TGGGTGAGAA AACGGAGAGA AGGAACTCTC CGAAGAAGGA CGTCGCGACC 
CTTGAAGCGA CTCACGAGAC CCGCACTGAG TGCCTGCTAG TTAAGCCAGA AAAGCAGACG 
ACAGCGTATC AAGAGGCTTC GGACCAGCCC GCCGTCGATG ACGACGAAAG CAAGCAGGTG 
CGTTCGGCAT CAGACGATCT TGCTCTCTCA CCCACGACGA ATTCTGTTCT CGACAAAGAC 
GAACAGTCCC CCGAGGAAGA TGGACAGACC CTCTCCGCTC CTTGTGCCTC CACCCTCGAG 
ACGTCGGAGG CTGCAGTGGA AGAGCCTGCC AGTGTCGAAG ACGAGGAACC ATCGCCTGGA 
TGCGACAGTG CCGATCCGGA GATCAGGGAG CCAGGAAGCA AGCAACCAGG GGGTTCTCTA 
CCTCAGGACG GACAGACAGA GCGCGCTGTA GAGCAGAAGA TTGAAACGTT TTCGAGTGCT 
TTGGTAGAAC GGGAGAGAGA AGATGTGACA GTCACGAGCT GTGCGGGAGG AGAAACTGTT 
GCCAACGCTT CGCAGTGCCC TGAGGACCAT TCGGACCTCT CTTTGTTGAA CAAGGAGGAA 
GACGCAGCGA ACATCGCGAC CCGTCTAGCA GAAACCCTGC AGTCACTGCC AGCAATCTCT 
GAGTGCACGG AGAGTCAGAC GGAAGACCAG GACCCCGAGA GCTCCGGGGA CGCTTCGTTC 
GTGGTCGAGG CGGAGTCTGC AGCGCGTGTG GCTCCGGAGG CTTCTGCCGC CTCCCAAGTT 
GTATTCGCGG GTGAGGAGGT TGTCGATTCC CTGGATTCCG AGTTGGCCGC GCTTGCAGAG 
ATATCTGCGG AAGTGGAAGA GTGTGGAGAA AACGCGGAAG AGGGCGAAGC AGAGGAATCG 
TGTGTCCTGG AGACCGATCC GTCAGCAGAA GCGCGAGAAA AGGAGCTCAT AAGCGACTTG 
GCGTCCCCGT TCTGGGCGTC GCCCCCGGCG TTGACTGCGC GTCAGCCCCG GTGTACTGCA 
AAGAAGGCGG TTTCCTTCTC TCCGTGGAAT GAGGAGAGGC CGACGGAGGC AGGTGAGGCG 
ACAGAGGCCG CGCGCGAGGT CGAGCTAGGC CTGGCAGCCC AAAGTGGGTC CGAGCTCGGG 
AAGAGTCCCG AGGCGGAACG GGCGGGAGAG GAAGCGGAGC CAGACTTTGG AGACGGTCTG 
CAGCGGTCGC ACCGAGCGGC GCTGTCTCCA GCGCTGGCTC TTTTGTACGA CGAGGACGTG 
TCAGTGTCGG AGGAGATTTT ATCCTGTCAG TCTTTGACGG ACGTCTCGCT GATGTCTCGT 
TCGCCCTCGA CATGCGATGG AAACGAACGG GCGCTGTCGC GGCCGACAGC TTCTCTGGAT 
TTGCGTGCGC GCGGGGAGAC CTTTATCGCA GGCCTCCCAG AGACTCGAGA CGAGGTCGAC 
GTGGAGCGCG ACTCCTCCTC CTCGAAGAGG ATTGCAACTC CAATGCACAT TGGCGTGGAC 
GTCGACGAGG GCGGCATCGC GGGAAACGTG AAGTCGTTCT GCAGTCACGC GTCTCTCGCT 
TCTTCCTTCC TGTTGGAGAG CGAGGACGAC CGGAGTGACC AGGCCTCGGG GGTGAATGGC 
TGCTTGGAGG CGACGTTCGA GAGCGAAGAG CCTCCGCTCC ATACCACCTT CATGGCTGAG 
CAGCTCCGTC TGCCGCTCGC GGCGCTGACG GAGGGCTCAG AGCTCGTCGC GCAGGAACCC 
GAGGTCGAGA AGGTTCTCTG GAAAGTCGAA GACACGCAGA ACACGACAGC GGAGGATTGT 
GCTTTCTCTT CGGTCTCCTC GAACGCGAGT CTCTTCCGCT CTCAGTCCCC CGAGACGCGC 
ATCGCCGGCG CCCCAGCGCC TGCGCCGGGC GCCCCCAGCC TCTGCCTCCC AGTGGCAGCC 
TTCCCGGGGT TCGCGGGCAC AGAAGCACCA TTCCCCTTTG TCGCTTCTGT CCCGAACGGC 
GTGCGAGACT CGCGTCTCGC GTTCGGTGTG GGGTCCATCG AGCGTTTGCT CCGCGTCGAG 
GATGAGACTA AGCAGTCTGG CCCTCCGGGG CTCCGCGGCT TCCCCGCCGG CCACTCTGAG 
ACCGCGAGCG AGGCGAACTT CCCGTTCGGA AGGTTCATCT CAGAGTCCCT CTCGTTCTCC 
CCGACGGTTT CCTTCGGATC GGGGCCCCTC GGGTTCCAGT CCGCCCCCGC GTGCGGCCTG 
AACATCGAAG AGCTCCTCGA CCTCGACAGT TTGGATGCGC AGGACCGGGA TGATGTGCGG 
CAACTCAAGA AGCAACTGGG TCACGAGTCC GACGACGCCG CCATCCCCCA GGCTGCGACA 
CCCTTCGTCC TCCCAGACGA CCCTGCGGGC TCTGCCTCCC GCGCAGGCGC CCCGGCCTTC 
CCGCCCCCTC CACCCCTTAG GGCCCCACGC GCCACTGCGG CTCCGCCCGA AAACTGCCGG 
GCCTCCTCTG GGCCCCAGAG GCCCCACGCG GCGTCACTGC GCGCCCCGCC GCCGCCTGTC 
TCGCCCCCGC AGGACGAGGA GGCCCCGCCG CCGGCTACGG TGACCTCTCA ACCGAACAGC 
TCGAAGCAGC GGATCGCGCT GCAGCACTAC ATGGCGCTCG TTCGCGAGTG CCACCGCACC 
AAGGACAGTC GCGGGGCGAT TCGCGTTCTC GGGCGACTCC AAGCTGAAGG TCGCGTTGCT 
GCTGACACCC AGCTCCTCAA TTACGTGCTG ATGGTTTGTG TCTCCGCGAA CGACCGCGTC 
ATGACAGGGC AGTTGTTCAA GTTCATGGAG ACGACGGGGT GCGCAGATTT GGTGACGTAC 
AACACACTGG TGAAGAGCTT CACGGCAAAC GGGGAACTGC ATGCGGCAGA GCAGGTGCTT 
GCTCGAATGG AGGGTCGCGA GAACACGTTG GAGGCGGAGC TGTCGGGTGC ACATCTGGGG 
ATGGGTTTCG AGGGCAAGTC GGAGAACGGG GTTTTGAATA GACCGAAGAT CTCTCCGGAC 
GAGGTGACTT TCAATCTGTT GGTGAACGCG GCGGTGAGCG CAGGCGACCC CGACAAGGCA 
TGGGAGTACA TTGACCGGAT TAAGAAGGCG AATCTGCGTC CGGACAAGTT CACGATTAGT 
TCGATCATCA AGTCGCTGCA GCCGGGACAG AACGAGCAGC ACACGCGGCG CGCGTTCGAG 
TTGATGGATC AGATCGATGC GTGCGAAGAC CTTGTTCTCC TCAGCACCTG CATCGATGCA 
TGCGCGCGGC TGGGTGACCT GGACCGACTC GCCACACTCC TCGCGCGCTT CGAAGACTCC 
GACTTGAAGC CGAACGCGCA TGCGTACGGG ACGCTAATCA AGGCGTACGG GAAGCTCGGC 
AACGTGACCC GTGTCTGGGA GCTTTGGATG GAGCAGCAGC GCAGTGGCAT CGAGGCCTCG 
AACTACACAC TCGGGTGCAT GATCGACTGT CTCGCGAGCA ATGGATTGAT GAACGAAGCC 
GTCGACCTGT TTGGGAAGAG CGTCGCGGCG GGGTCTGCCG ACGCGGTGCT CTTCTCCATT 
CTGGTCAAGG GCCTCGCTCG ATTCCGAAAT CGCGGCCTGG AGGTTGCCCT CGGAGCGTAC 
CGGCGCCTGA AGGAGAGCGA CAACTCGGCC GCGGTGACTC CTGCTTCGAA GCCTGCTGCG 
GGGAGCCGCG CGCCAACCGG AGGCCCGTCT TGTGGGGTGG ACGGTAGCCG TGAGAGTCGG 
AGACGCGGAG GCAGCAAGCG CCCCGTGGGG GGCGAGAAGG GGGACAAAGC CGGGGCTGCG 
CGCGATGGTG ACGGGGAGGA GCATAGCTGC TATCTGAAGG CTCTCAACAC CATTTCCTTT 
AACTCGCTCC TCCACCTCTG CGTGCGCTCC GGACGCCTGG CGGACGCTCT CGAACTCTTC 
AAAGACATGC AGTCCCACCC GCATGCGCAG CCGGATCTCA TCACCTACAG CACAGTCATC 
AAGGGCCTCT GTTGCTCCTC CAAGGAGCAC GCGCTAGACG CCGCCTTGGG CCTGTTCGAG 
CAGATGCAGA AGGACGCGAA GATCTCCCCA GATGCAATTG TGTACAATAC CCTCATCCAG 
GGCGCGGCCA CTCGCAGAAA CGTCACTCTC GTTGAGTCGT TGCTTCTCCA CATGCTCCAA 
AACAAAGTGA AACCCAGCAG CTACACGCTG TGTCAGTGCG TCAAGCTCTA TGGCAAGTGC 
GGTGACCTCG CTCGCGCCCT GGAGCTTGCG CTCGAGCTTC CACGTCGTTT CAACTTCGCG 
ATGGACAGCT ACGTGTACAC GGCGCTGATC GCTGCCTGCA CACACAACCG GGCGCCGTTC 
CTGGCGGCGA CGCTCGCCCT GCAGGCGACG CAGGAGAACC AGGAGTTACC TCCTGCTTTG 
CTTCTGCGTC TGGCGAACCA TCTGCAGCAG CAGCGGGAGC TGGCCGAGAG AGAAGCGATG 
AACTCGAGCT TGCGAGAGTT GCCACTGCTT CAGAACGTGT GTTCGAGCGA GCTGAATGGA 
AAGGTTCTCG GTCCGTACGG GGTCCGAAAA TTGTCCCCGG CGGAGGAGGC GAAGGCGACT 
GTCGACGCCA TCATCAAGTT GCTGAAGCGC GCGGTCGGAA GCACCGGGGC TCCGGGGGCG 
AGGGAGGACC GCGAAGGTTC TGGGGAGGCC CTCTCATTCG CGTCTTTCTC GTCTCCTGGC 
GACAGTTCAC AGAGAGATGG GGGTCGCGGG GCCTTCGCGA CGTCGCGCAC CGCCGCTGGC 
AGCAAGCGGT CGAAGCGTGG TGGCGGCCAG TCCGTGGTTC CCGTTGTGGA CCGAGAGCGC 
GGCGAGAGAG TGGAGAAGGC GACCGCGTTC TCCGGGGTAA ACGGCAAGCG AAAGCCGGGA 
AACAAGGTCC AGGAGACCAG GGCGGCTGCG GACGACGGGG GCCCTCCAGG TCTGCATGCA 
GGAGCGACAA GCCGCGGGTT TGGCGGGCGC CAGGGTGGCG AGAGCGGCCC GAAGACATTT 
GCTGGGCAGC GCAAGCAGCT GGTGAACGCG GGGACGCCGC CTCCACCGCA GACTGCGTTC 
AAAAAGAGCT CGGTGCATGC AAGTCGAGTG GGGGGTTTGC CGCGAGGCAG CGGCGGACTG 
GAGAGCACGA ACTTGTACCC CTTTGAGGAC ATGGGACTGA ATTTGGATTT CGATGGCCCG 
CGCATGCGTG GGAGACACAG CAGCGGGGCG TCGAGCGCCG TCACGACTGC CAGCTTCTCA 
AAAGAAACCG CGAGAAGCGG GCGGAAGGGA CATGCCAACA ACGGGCGAGG GGGTCGTCGA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TGME49_250760 cDNA ORF clone, Toxoplasma gondii ME49

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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