ORF » Species Summary » Thalassiosira pseudonana CCMP1335 » DNA_TopoIV cDNA ORF clone

DNA_TopoIV cDNA ORF clone, Thalassiosira pseudonana CCMP1335

The following DNA_TopoIV gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DNA_TopoIV cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OTf05392 XM_002288732.1
Latest version!		 Thalassiosira pseudonana CCMP1335 DNA topoisomerase IV, ATP/GTP binding site (DNA_TopoIV), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    DNA_TopoIV ( XM_002288732.1 ) cDNA ORF clone, Thalassiosira pseudonana CCMP1335 -> XP_002288768.1 Thalassiosira pseudonana CCMP1335 DNA topoisomerase IV, ATP/GTP binding site (DNA_TopoIV), mRNA.
    Price & Availability↑
    CloneID OTf05392
    Clone ID Related Accession (Same CDS sequence) XM_002288732.1
    Accession Version XM_002288732.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2463bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497456000000
    Organism Thalassiosira pseudonana CCMP1335
    Product DNA topoisomerase IV, ATP/GTP binding site
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_012066). 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCAGTATG CGTTATCAAT CATATTGGGG AGAGCACTTC CCGATGCAAG GGATGGAATG 
    AAACCCGTGC ATAGGAGGAT ATTGTATGCG ATGAATGGGT TGGGATTGAG TCCTAGTGGG 
    AGTTATAGGA AGTGTGCCAG GGTTGTTGGT GAGGTGTTGG GCAAATACCA TCCTCATGGA 
    GATACTGCCG TGTACGATGC GTTGGTTCGT CTAGCTCAGC CGTTCTCTAC CAACATTCCA 
    CTGATTGATG GACATGGCAA CTTTGGAAGT ATCGATAATG ATCCCGCGGC AGCTATGCGA 
    TATACGGAAT GTCGTTTGAC TCGCATCGCT GCTGAAACAT TACTGGATGA CATCCTAATG 
    GACACAGTGG AGTTTCTACC AAACTTTGAC GGAAGCGAGT ACGAACCGAG TGTACTTCCG 
    GCAAAGGTTC CCATTCTACT CTTGAATGGA GGTGCGGGGA TTGCAGTGGG TATGGCGACA 
    AATGTACCTC CTCATAACTT GGGTGAATTG ATGGATGCAT GTGTGGCATT GACGAAGGGG 
    AGGAACGAGG GCGGGGAGGA GGTTACGGAT GGAAAGTTGC TGCAGATTAT TCCTGCCCCC 
    GATTTCCCGA CCGGAGCATG CATCATTGGA AAGTCTGGAT CTCGGAGTTT GTACACAACC 
    GGCAATGGAG GAGTGGTAAT GCGTGCAGTA ATGCATTTGG AAGAAGTACC CACCTCGTCT 
    GGAAAGAAGA AATCAACACG TAATGCAATC ATTGTCACAG AATTACCCTA CCAGGTGAAC 
    AAAGCCGCTC TGTTAGAGCG TATTGCAATT CTTGTCAACG AAAAGAAACT GGAAGGGATT 
    GCAGACTTGC GTGATGAGAG TGATCGTGAC GGAATTAGGA TGGTGATTGA GTTGAAACGA 
    GATGCAGTTG CTGCGGTGGT ACAGAACAAC TTGCTGAAAA AGACTTCATT GCAGACTACC 
    TTCTCGGGTA ACTTCTTAGC CTTATTTGGT GGTGGAACCA ATCCCCAGCG GTTTACGCTG 
    CGTGAGGCTT TGGACTGCTT CCTTGACTTT CGCTTCCAAA CCATACGCAA CAAGTGTGCC 
    TTCCAGTTGG ATAAGGTGGA GAACAGGGCT CACATTGTTG CTGGATTATT GATTGCTCTT 
    GAATCTACCG ACGAGGTCAT TGCAATCGTG AGAAATGCTC CAGATCAAGC ATCGGCCCGT 
    GCGGCTCTCA TGAGTACTGA CAATCCTAAA CTTGGTCTTT CCAGTGCACA AGCAGATGCC 
    GTTCTCAAAT TGCAGCTCGG TCAGTTGACT CGGTTGAATG GAGACAAACT CAATGATGAG 
    AAAAAGACAT TGGATGAAAG TCAGACGAAT CTTAGAAACC TTCTAACCAA TGATGCTGCC 
    GTGAGGGATA CAATGTTAGA GGAGTTTATG GCGATGAAGA AGCGATTTGG TACTCCTCGT 
    AAGACAAAGA TTCTCCCCGA CGAAGAAGAC AAGGCAGACA TCGATTTGGT ACAGAATGAA 
    CGATCTGTCA TTGTTGTTAC GAGAGGTGGA TACATCAAGC GAATGCCGTT GAAGACGTTT 
    GAAAGCCAGA ACCGTGGGAC TCGTGGAAAG AGAGGTACTT CGAACACGAT TTCGGACGAC 
    AACGCAGTAG CGCACTGCTT CACTTGCAAC GACCACGATA CTGTGTTGAT GACAACTCAA 
    AGCGGAGTTG CGTATGGGCT CCGTGCGTAC CAGGTACCAG AGGGTGGTAG AACCGCTCGT 
    GGTGCTCCTA TTCCCTCGGT GTTACCTGTC AAGTCGGACG ATGTCATCAC ATCTGTACTA 
    CCAGTCTCGG AGTTCAGTAA GAATGAGTTC ATTGTACTCG CAACAGAGTA TGGATGGATC 
    AAAAAGACAC CCCTCGCTGC TTTTGAGAAA CTCACTCAGC GTGGGCTCAT CATTGCAACA 
    TTAGCTGACG GGGATCGTCT CAACTGGTGT GAGAAGTGCA CCGACAACGA TGACATCCTG 
    GTGGGATCTA CTCGAGGTCA GGCTACACGG TTCAAAGCAG CTGATTTACG ACCTACGGCC 
    AGAACTAGCC GCGGCGTCAA GTCAATGACA CTCAAAAAGG GAGATACTAT CGCTGATATG 
    AATGTTTTGA GGAAAGAAGA CAAGTTCGTG CTCGTTGTCA CGACAGAGGG TTACGGCAAA 
    CGAGTGTTGA CGGAAGAGTT CCGCACTACA GCACGCGGTG GATCGGGCGT GATAGCAATC 
    AAATTCAAAG CTGGTCGCGT GGATGATAGG ATTAGCACAA TGCGGGTGGT TAATGAGGAC 
    GATGAGATCC TTCTTATTAC ATCTCAGGGG GTTATTGTAC GACAGAAGGT GTCGGATATT 
    TCATGTCAGG GGCGTGCTGC AACGGGAGTT CTCGTGCAAA AGGTTGACGT GAGGAGTGGA 
    GATTCAATCT CAACTGTTAG CATCGTACCC AAAGAGGACG CGGATGGAGA TGTAACTCAA 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002288768.1
    CDS34..2496
    Translation

    Target ORF information:

    RefSeq Version XM_002288732.1
    Organism Thalassiosira pseudonana CCMP1335
    Definition Thalassiosira pseudonana CCMP1335 DNA topoisomerase IV, ATP/GTP binding site (DNA_TopoIV), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_002288732.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCAGTATG CGTTATCAAT CATATTGGGG AGAGCACTTC CCGATGCAAG GGATGGAATG 
    AAACCCGTGC ATAGGAGGAT ATTGTATGCG ATGAATGGGT TGGGATTGAG TCCTAGTGGG 
    AGTTATAGGA AGTGTGCCAG GGTTGTTGGT GAGGTGTTGG GCAAATACCA TCCTCATGGA 
    GATACTGCCG TGTACGATGC GTTGGTTCGT CTAGCTCAGC CGTTCTCTAC CAACATTCCA 
    CTGATTGATG GACATGGCAA CTTTGGAAGT ATCGATAATG ATCCCGCGGC AGCTATGCGA 
    TATACGGAAT GTCGTTTGAC TCGCATCGCT GCTGAAACAT TACTGGATGA CATCCTAATG 
    GACACAGTGG AGTTTCTACC AAACTTTGAC GGAAGCGAGT ACGAACCGAG TGTACTTCCG 
    GCAAAGGTTC CCATTCTACT CTTGAATGGA GGTGCGGGGA TTGCAGTGGG TATGGCGACA 
    AATGTACCTC CTCATAACTT GGGTGAATTG ATGGATGCAT GTGTGGCATT GACGAAGGGG 
    AGGAACGAGG GCGGGGAGGA GGTTACGGAT GGAAAGTTGC TGCAGATTAT TCCTGCCCCC 
    GATTTCCCGA CCGGAGCATG CATCATTGGA AAGTCTGGAT CTCGGAGTTT GTACACAACC 
    GGCAATGGAG GAGTGGTAAT GCGTGCAGTA ATGCATTTGG AAGAAGTACC CACCTCGTCT 
    GGAAAGAAGA AATCAACACG TAATGCAATC ATTGTCACAG AATTACCCTA CCAGGTGAAC 
    AAAGCCGCTC TGTTAGAGCG TATTGCAATT CTTGTCAACG AAAAGAAACT GGAAGGGATT 
    GCAGACTTGC GTGATGAGAG TGATCGTGAC GGAATTAGGA TGGTGATTGA GTTGAAACGA 
    GATGCAGTTG CTGCGGTGGT ACAGAACAAC TTGCTGAAAA AGACTTCATT GCAGACTACC 
    TTCTCGGGTA ACTTCTTAGC CTTATTTGGT GGTGGAACCA ATCCCCAGCG GTTTACGCTG 
    CGTGAGGCTT TGGACTGCTT CCTTGACTTT CGCTTCCAAA CCATACGCAA CAAGTGTGCC 
    TTCCAGTTGG ATAAGGTGGA GAACAGGGCT CACATTGTTG CTGGATTATT GATTGCTCTT 
    GAATCTACCG ACGAGGTCAT TGCAATCGTG AGAAATGCTC CAGATCAAGC ATCGGCCCGT 
    GCGGCTCTCA TGAGTACTGA CAATCCTAAA CTTGGTCTTT CCAGTGCACA AGCAGATGCC 
    GTTCTCAAAT TGCAGCTCGG TCAGTTGACT CGGTTGAATG GAGACAAACT CAATGATGAG 
    AAAAAGACAT TGGATGAAAG TCAGACGAAT CTTAGAAACC TTCTAACCAA TGATGCTGCC 
    GTGAGGGATA CAATGTTAGA GGAGTTTATG GCGATGAAGA AGCGATTTGG TACTCCTCGT 
    AAGACAAAGA TTCTCCCCGA CGAAGAAGAC AAGGCAGACA TCGATTTGGT ACAGAATGAA 
    CGATCTGTCA TTGTTGTTAC GAGAGGTGGA TACATCAAGC GAATGCCGTT GAAGACGTTT 
    GAAAGCCAGA ACCGTGGGAC TCGTGGAAAG AGAGGTACTT CGAACACGAT TTCGGACGAC 
    AACGCAGTAG CGCACTGCTT CACTTGCAAC GACCACGATA CTGTGTTGAT GACAACTCAA 
    AGCGGAGTTG CGTATGGGCT CCGTGCGTAC CAGGTACCAG AGGGTGGTAG AACCGCTCGT 
    GGTGCTCCTA TTCCCTCGGT GTTACCTGTC AAGTCGGACG ATGTCATCAC ATCTGTACTA 
    CCAGTCTCGG AGTTCAGTAA GAATGAGTTC ATTGTACTCG CAACAGAGTA TGGATGGATC 
    AAAAAGACAC CCCTCGCTGC TTTTGAGAAA CTCACTCAGC GTGGGCTCAT CATTGCAACA 
    TTAGCTGACG GGGATCGTCT CAACTGGTGT GAGAAGTGCA CCGACAACGA TGACATCCTG 
    GTGGGATCTA CTCGAGGTCA GGCTACACGG TTCAAAGCAG CTGATTTACG ACCTACGGCC 
    AGAACTAGCC GCGGCGTCAA GTCAATGACA CTCAAAAAGG GAGATACTAT CGCTGATATG 
    AATGTTTTGA GGAAAGAAGA CAAGTTCGTG CTCGTTGTCA CGACAGAGGG TTACGGCAAA 
    CGAGTGTTGA CGGAAGAGTT CCGCACTACA GCACGCGGTG GATCGGGCGT GATAGCAATC 
    AAATTCAAAG CTGGTCGCGT GGATGATAGG ATTAGCACAA TGCGGGTGGT TAATGAGGAC 
    GATGAGATCC TTCTTATTAC ATCTCAGGGG GTTATTGTAC GACAGAAGGT GTCGGATATT 
    TCATGTCAGG GGCGTGCTGC AACGGGAGTT CTCGTGCAAA AGGTTGACGT GAGGAGTGGA 
    GATTCAATCT CAACTGTTAG CATCGTACCC AAAGAGGACG CGGATGGAGA TGTAACTCAA 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The Phaeodactylum genome reveals the evolutionary history of diatom genomes.
    Nature456(7219)239-44(2008 Nov)
    Bowler C,Allen AE,Badger JH,Grimwood J,Jabbari K,Kuo A,Maheswari U,Martens C,Maumus F,Otillar RP,Rayko E,Salamov A,Vandepoele K,Beszteri B,Gruber A,Heijde M,Katinka M,Mock T,Valentin K,Verret F,Berges JA,Brownlee C,Cadoret JP,Chiovitti A,Choi CJ,Coesel S,De Martino A,Detter JC,Durkin C,Falciatore A,Fournet J,Haruta M,Huysman MJ,Jenkins BD,Jiroutova K,Jorgensen RE,Joubert Y,Kaplan A,Kröger N,Kroth PG,La Roche J,Lindquist E,Lommer M,Martin-Jézéquel V,Lopez PJ,Lucas S,Mangogna M,McGinnis K,Medlin LK,Montsant A,Oudot-Le Secq MP,Napoli C,Obornik M,Parker MS,Petit JL,Porcel BM,Poulsen N,Robison M,Rychlewski L,Rynearson TA,Schmutz J,Shapiro H,Siaut M,Stanley M,Sussman MR,Taylor AR,Vardi A,von Dassow P,Vyverman W,Willis A,Wyrwicz LS,Rokhsar DS,Weissenbach J,Armbrust EV,Green BR,Van de Peer Y,Grigoriev IV


    The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism.
    Science (New York, N.Y.)306(5693)79-86(2004 Oct)
    Armbrust EV,Berges JA,Bowler C,Green BR,Martinez D,Putnam NH,Zhou S,Allen AE,Apt KE,Bechner M,Brzezinski MA,Chaal BK,Chiovitti A,Davis AK,Demarest MS,Detter JC,Glavina T,Goodstein D,Hadi MZ,Hellsten U,Hildebrand M,Jenkins BD,Jurka J,Kapitonov VV,Kröger N,Lau WW,Lane TW,Larimer FW,Lippmeier JC,Lucas S,Medina M,Montsant A,Obornik M,Parker MS,Palenik B,Pazour GJ,Richardson PM,Rynearson TA,Saito MA,Schwartz DC,Thamatrakoln K,Valentin K,Vardi A,Wilkerson FP,Rokhsar DS


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