B2R cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	B2R
Entrez Gene ID	397277
Full Name	bradykinin type 2 receptor
Synonyms	Bdkrb2
Gene Type	protein-coding
Organism	Sus scrofa(pig)

mRNA and Protein(s)

mRNA	Protein	Name
NM_214146.1	NP_999311.1	B2 bradykinin receptor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

β-blockade abolishes the augmented cardiac tPA release induced by transactivation of heterodimerised bradykinin receptor-2 and β2-adrenergic receptor in vivo.
Aspelin T, Eriksen M, Ilebekk A, Cataliotti A, Carlson CR, Lyberg T
Thrombosis and haemostasis112(5)951-9(2014 Nov)

Bradykinin induces NO and PGF2α production via B2 receptor activation from cultured porcine basilar arterial endothelial cells.
Islam MZ, Miyagi K, Matsumoto T, Nguyen HT, Yamazaki-Himeno E, Shiraishi M, Miyamoto A
Naunyn-Schmiedeberg's archives of pharmacology387(7)697-702(2014 Jul)

Neuronal and non-neuronal bradykinin receptors are involved in the contraction and/or relaxation to the pig bladder neck smooth muscle.
Ribeiro AS, Fernandes VS, Martínez MP, Martínez-Sáenz A, Pazos MR, Orensanz LM, Recio P, Bustamante S, Carballido J, García-Sacristán A, Prieto D, Hernández M
Neurourology and urodynamics33(5)558-65(2014 Jun)

Localization of bradykinin B(2) receptor in the follicles of porcine ovary and increased expression of matrix metalloproteinase-3 and -20 in cultured granulosa cells by bradykinin treatment.
Kimura A, Kihara T, Ohkura R, Ogiwara K, Takahashi T
Biology of reproduction65(5)1462-70(2001 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following B2R gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the B2R cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe00142	NM_214146.1 Latest version!	Sus scrofa bradykinin type 2 receptor (B2R), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

B2R ( NM_214146.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_999311.1 Sus scrofa bradykinin type 2 receptor (B2R), mRNA.

Price & Availability↑

CloneID	OSe00142
Clone ID Related Accession (Same CDS sequence)	NM_214146.1
Accession Version	NM_214146.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1509120000000
Organism	Sus scrofa(pig)
Product	B2 bradykinin receptor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB051422.1. ##Evidence-Data-START## Transcript exon combination :: AB051422.1 [ECO:0000332] ##Evidence-Data-END##

ATGCTCAACC TCACCTCGCA AGTCCCGGAG CCCGCTCTCA ATGGGACCCT TCCCCAGAGC 
AGCAGCTGCT TCCACTCGGA CTGGTGGAAC TGGCTCAACA CCATCCAGGC CCCCTTCCTC 
TGGGTCCTCT TCCTCCTGGC AGCTCTCGAG AACATCTTTG TCCTCAGCGT CTTCTGCCTG 
CACAAGAACA GCTGCACGGT GGCGGAGATC TACCTGGGCA ACCTGGCCAT GGCGGACCTG 
ATCTTGGCCT TGGGACTGCC CTTCTGGGCC ATCACCATCG CCAACCACTT CGACTGGCTC 
TTCGGGGAGG TCCTCTGCCG CGTGGTGAAC ACCATGATCT ACATGAACCT CTACAGCAGC 
ATCTGCTTCC TCATGCTGGT CAGCATCGAC CGCTACCTGG CCCTGGTGAA GACCATGTCC 
ATGGGCCGGA TGCGCGGGGT GCGCTGGGCC AAGCTCTACA GCCTGGTGAT CTGGGGCTGC 
ACGCTGCTCC TGAGCTCGCC CATGCTGGCC TTCCGGACCA TGCACGAGTA CGCAGCCGAG 
GGCCACAACG TCACCGCCTG CATCATCAAG TACCCCTCGC GCAGCTGGAT GGTGTTCACC 
AACATCCTCC TCAACTCCGT GGGCTTCCTG CTGCCCCTGA GCATCATCAC CTACTGCACC 
GTGCAGATCC TGCAGGTGCT GCGCAACAAC GAGATGCAGA AGTTCAAGGA GATCCAGACG 
GAGAGGAAGG CCACGGTGCT GGTCCTGGCC GTGCTGCTGC TGTTCGTCGT CTGCTGGCTG 
CCCTTCCAGA TCAGCACCTT CCTGGACACG CTGCTGCGCC TCGGCGTGCT GTCCGGATGC 
TGGGACGAGC ACGCCGTAGA CGTCATCACG CAGATCAGTT CCTACGTGGC CTACAGCAAC 
AGCGGCCTCA ACCCACTGGT GTACGTGATC GTGGGCAAGC GCTTCCGGAA GAAGTCCCGA 
GAGGTGTACC GGGTGCTGTG CCAGAAAGGA GGCTGCATGG GAGAACCCGT CCAGATGGAG 
AACTCCATGG GGACTTTGAG AACCTCGATC TCCGTGGAAC GGCAGATCCA CAAGCTGCAG 
GACTGGGCAG GGAAGAAACA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999311.1
CDS	228..1331
Translation	MLNLTSQVPEPALNGTLPQSSSCFHSDWWNWLNTIQAPFLWVLFLLAALENIFVLSVFCLHKNSCTVAEIYLGNLAMADLILALGLPFWAITIANHFDWLFGEVLCRVVNTMIYMNLYSSICFLMLVSIDRYLALVKTMSMGRMRGVRWAKLYSLVIWGCTLLLSSPMLAFRTMHEYAAEGHNVTACIIKYPSRSWMVFTNILLNSVGFLLPLSIITYCTVQILQVLRNNEMQKFKEIQTERKATVLVLAVLLLFVVCWLPFQISTFLDTLLRLGVLSGCWDEHAVDVITQISSYVAYSNSGLNPLVYVIVGKRFRKKSREVYRVLCQKGGCMGEPVQMENSMGTLRTSISVERQIHKLQDWAGKKQ

Target ORF information:

RefSeq Version	NM_214146.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa bradykinin type 2 receptor (B2R), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214146.1

ORF Insert Sequence:

ATGCTCAACC TCACCTCGCA AGTCCCGGAG CCCGCTCTCA ATGGGACCCT TCCCCAGAGC 
AGCAGCTGCT TCCACTCGGA CTGGTGGAAC TGGCTCAACA CCATCCAGGC CCCCTTCCTC 
TGGGTCCTCT TCCTCCTGGC AGCTCTCGAG AACATCTTTG TCCTCAGCGT CTTCTGCCTG 
CACAAGAACA GCTGCACGGT GGCGGAGATC TACCTGGGCA ACCTGGCCAT GGCGGACCTG 
ATCTTGGCCT TGGGACTGCC CTTCTGGGCC ATCACCATCG CCAACCACTT CGACTGGCTC 
TTCGGGGAGG TCCTCTGCCG CGTGGTGAAC ACCATGATCT ACATGAACCT CTACAGCAGC 
ATCTGCTTCC TCATGCTGGT CAGCATCGAC CGCTACCTGG CCCTGGTGAA GACCATGTCC 
ATGGGCCGGA TGCGCGGGGT GCGCTGGGCC AAGCTCTACA GCCTGGTGAT CTGGGGCTGC 
ACGCTGCTCC TGAGCTCGCC CATGCTGGCC TTCCGGACCA TGCACGAGTA CGCAGCCGAG 
GGCCACAACG TCACCGCCTG CATCATCAAG TACCCCTCGC GCAGCTGGAT GGTGTTCACC 
AACATCCTCC TCAACTCCGT GGGCTTCCTG CTGCCCCTGA GCATCATCAC CTACTGCACC 
GTGCAGATCC TGCAGGTGCT GCGCAACAAC GAGATGCAGA AGTTCAAGGA GATCCAGACG 
GAGAGGAAGG CCACGGTGCT GGTCCTGGCC GTGCTGCTGC TGTTCGTCGT CTGCTGGCTG 
CCCTTCCAGA TCAGCACCTT CCTGGACACG CTGCTGCGCC TCGGCGTGCT GTCCGGATGC 
TGGGACGAGC ACGCCGTAGA CGTCATCACG CAGATCAGTT CCTACGTGGC CTACAGCAAC 
AGCGGCCTCA ACCCACTGGT GTACGTGATC GTGGGCAAGC GCTTCCGGAA GAAGTCCCGA 
GAGGTGTACC GGGTGCTGTG CCAGAAAGGA GGCTGCATGG GAGAACCCGT CCAGATGGAG 
AACTCCATGG GGACTTTGAG AACCTCGATC TCCGTGGAAC GGCAGATCCA CAAGCTGCAG 
GACTGGGCAG GGAAGAAACA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

β-blockade abolishes the augmented cardiac tPA release induced by transactivation of heterodimerised bradykinin receptor-2 and β2-adrenergic receptor in vivo.
Thrombosis and haemostasis112(5)951-9(2014 Nov)
Aspelin T,Eriksen M,Ilebekk A,Cataliotti A,Carlson CR,Lyberg T

Bradykinin induces NO and PGF2α production via B2 receptor activation from cultured porcine basilar arterial endothelial cells.
Naunyn-Schmiedeberg's archives of pharmacology387(7)697-702(2014 Jul)
Islam MZ,Miyagi K,Matsumoto T,Nguyen HT,Yamazaki-Himeno E,Shiraishi M,Miyamoto A

Neuronal and non-neuronal bradykinin receptors are involved in the contraction and/or relaxation to the pig bladder neck smooth muscle.
Neurourology and urodynamics33(5)558-65(2014 Jun)
Ribeiro AS,Fernandes VS,Martínez MP,Martínez-Sáenz A,Pazos MR,Orensanz LM,Recio P,Bustamante S,Carballido J,García-Sacristán A,Prieto D,Hernández M

Localization of bradykinin B(2) receptor in the follicles of porcine ovary and increased expression of matrix metalloproteinase-3 and -20 in cultured granulosa cells by bradykinin treatment.
Biology of reproduction65(5)1462-70(2001 Nov)
Kimura A,Kihara T,Ohkura R,Ogiwara K,Takahashi T

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B2R cDNA ORF clone, Sus scrofa(pig)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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