PRPF8 cDNA ORF clone, Sus scrofa(Pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRPF8
Entrez Gene ID	100526074
Full Name	pre-mRNA processing factor 8
Gene Type	protein-coding
Organism	Sus scrofa(Pig)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021067568.1	XP_020923227.1	pre-mRNA-processing-splicing factor 8
XM_021067569.1	XP_020923228.1	pre-mRNA-processing-splicing factor 8
XM_003131826.4	XP_003131874.4	pre-mRNA-processing-splicing factor 8

Pathways from BioSystems
Gene Ontology

The following PRPF8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRPF8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe234277	XM_021067568.1 Latest version!	Sus scrofa pre-mRNA processing factor 8 (PRPF8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSe234277	XM_021067569.1 Latest version!	Sus scrofa pre-mRNA processing factor 8 (PRPF8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSe41344	XM_003131826.4 Latest version!	Sus scrofa pre-mRNA processing factor 8 (PRPF8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRPF8 ( XM_021067568.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_020923227.1 Sus scrofa pre-mRNA processing factor 8 (PRPF8), transcript variant X1, mRNA.

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CloneID	OSe234277
Clone ID Related Accession (Same CDS sequence)	XM_021067568.1 , XM_021067569.1
Accession Version	XM_021067568.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	pre-mRNA-processing-splicing factor 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010454.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGAG TGTTTCCTTA CCGAGGGCCG GGCAACCCGG TTCCCGGCCC TCTAGCTCCG 
CTGCCAGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CTCGAAAATG GCAGCAGTTG 
CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCTCA GAAGGAAGAC 
ATGCCTCCAG AACATGTTAG GAAGATCATT CGAGACCATG GGGACATGAC CAACAGGAAG 
TTTCGCCATG ACAAAAGGGT CTACCTGGGT GCTCTCAAGT ACATGCCCCA TGCAGTCCTC 
AAACTCCTGG AGAACATGCC GATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTATAC 
CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATTCCCT GGGTCATTGA GCCTGTCTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGAG AAAAAAGAGA CAGGAGGCAT 
TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CGCCTTTGGA CTATGCTGAC 
AACATCCTAG ATGTCGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGATCC TGAGGAGGAT 
GCCCCGGTGT TAGACTGGTT CTATGACCAC CAGCCGTTGA GGGACAGCCG AAAGTATGTG 
AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACACTGCCCA TGATGTCCAC TCTCTACCGC 
CTGGCCAATC AGCTTCTGAC AGACTTGGTA GATGACAACT ACTTCTATCT ATTTGATCTG 
AAGGCCTTTT TTACCTCCAA GGCACTCAAT ATGGCCATTC CTGGAGGCCC CAAGTTTGAG 
CCTCTTGTCC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGATATTAAC 
AAGATCATCA TCCGGCAGCC TATCCGCACT GAGTACAAGA TTGCCTTTCC TTACTTGTAC 
AACAACCTTC CACACCACGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACTGAGG ATCCTGACTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATATCC 
CACAGACACT CTGTCAAGAG CCAGGAACCG TTGCCGGATG ACGATGAAGA GTTTGAGCTC 
CCAGAGTTCG TGGAGCCCTT CCTGAAGGAC ACACCCCTCT ACACAGACAA CACAGCCAAT 
GGCATTGCCC TGCTCTGGGC CCCACGGCCC TTCAACCTAC GCTCTGGTCG CACCCGCCGG 
GCCCTAGACA TCCCCCTCGT CAAGAACTGG TATCGGGAGC ATTGTCCCGC TGGGCAGCCT 
GTGAAAGTGA GGGTCTCTTA CCAGAAGCTG CTTAAGTACT ATGTGCTGAA TGCCCTGAAG 
CACCGGCCCC CTAAAGCCCA AAAGAAGAGG TATCTGTTCC GTTCCTTCAA AGCCACCAAG 
TTCTTTCAGT CTACGAAGCT GGACTGGGTG GAGGTGGGGC TGCAGGTTTG CCGTCAGGGC 
TACAACATGC TCAACCTTCT TATTCACCGT AAAAATCTCA ACTATCTGCA TCTGGATTAC 
AACTTCAACC TCAAGCCCGT CAAGACGCTC ACCACCAAGG AAAGAAAGAA ATCTCGTTTT 
GGGAATGCTT TTCACCTGTG TCGAGAAGTT CTGCGTTTAA CTAAGCTGGT GGTGGACAGT 
CATGTGCAGT ATCGGTTGGG CAACGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAC 
ATCTTTGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGGT ACAAATACAA GCTGATGCGG 
CAGATAAGAA TGTGCAAGGA CCTGAAGCAT CTCATCTATT ACCGCTTCAA CACGGGCCCT 
GTGGGGAAGG GCCCTGGCTG TGGCTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTCTTC 
ATGCGTGGCA TCACCCCTTT GCTGGAGCGT TGGCTGGGCA ACCTCCTAGC TCGGCAGTTC 
GAAGGCCGGC ACTCAAAGGG GGTGGCAAAG ACTGTGACAA AGCAGCGCGT GGAATCGCAT 
TTTGACCTTG AGCTTCGGGC CGCTGTAATG CATGACATTC TGGACATGAT GCCGGAGGGA 
ATAAAACAGA ACAAGGCCCG GACCATCCTG CAGCACCTCA GTGAAGCCTG GCGCTGCTGG 
AAGGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCAACAC CTATAGAGAA TATGATCCTT 
CGATACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACTG CCCACTACAA CCGGGAACGA 
ATTCGCCGTG GGGCCACGGT AGATAAGACT GTTTGCAAAA AGAACCTGGG CCGCCTCACT 
CGGCTCTACC TGAAGGCAGA ACAGGAGCGG CAGCACAACT ACCTGAAGGA CGGGCCTTAC 
ATCACAGCAG AGGAAGCAGT GGCAGTATAT ACCACCACTG TGCATTGGTT GGAAAGCCGC 
AGGTTCTCAC CCATCCCCTT CCCCCCACTC TCCTACAAGC ATGACACCAA GTTGCTCATC 
TTAGCCTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CCCGGTTAAA CCAGTCTCAG 
AGGGAAGAGC TGGGTCTGAT CGAGCAAGCT TATGACAACC CCCATGAGGC GTTGTCCCGC 
ATCAAGCGTC ACCTTCTCAC ACAGAGAGCC TTCAAAGAAG TAGGCATTGA GTTCATGGAT 
CTCTATAGCC ACCTGGTGCC AGTGTATGAT GTGGAGCCCC TGGAGAAAAT CACTGATGCC 
TACCTGGACC AGTACCTCTG GTACGAAGCT GACAAGCGCC GCCTTTTCCC GCCCTGGATC 
AAGCCTGCTG ACACAGAACC GCCGCCTCTG CTTGTTTACA AGTGGTGCCA AGGCATCAAT 
AACCTGCAGG ACGTGTGGGA GACAAGCGAG GGCGAGTGCA ACGTCATGCT GGAGTCACGA 
TTTGAGAAGA TGTATGAGAA GATCGACCTG ACCCTGCTGA ACAGGCTGCT GCGCCTCATC 
GTAGACCACA ACATCGCTGA CTACATGACT GCCAAGAACA ACGTCGTCAT CAACTACAAG 
GACATGAACC ATACAAATTC GTATGGGATC ATCAGAGGCC TGCAGTTTGC CTCGTTCATC 
GTGCAGTACT ATGGCCTGGT GATGGATTTG CTTGTGTTGG GCTTGCACCG GGCCAGTGAG 
ATGGCTGGGC CCCCTCAGAT GCCAAACGAC TTTCTCAGTT TCCAAGACAT AGCTACTGAG 
GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATCGATC GTATCCATAT TTTTTTCAGG 
TTCACGGCAG ATGAGGCTCG GGACCTAATC CAGCGTTACC TGACGGAGCA TCCTGACCCC 
AACAATGAGA ACATCGTCGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA TGCCCGCATG 
CGCCTCATGA AGCATGATGT TAACTTGGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT 
CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCGGT GTACAGTAAG 
GACAACCCCA ACCTGCTGTT CAACATGTGC GGTTTTGAGT GCCGCATCCT GCCAAAATGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAATGAGGTT 
ACTAAGGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC 
CACAACCGTG TGCGTCAAAT TCTGATGGCC TCTGGGTCCA CCACCTTCAC CAAGATTGTG 
AATAAATGGA ACACAGCTCT TATTGGCCTT ATGACATACT TTCGGGAGGC TGTGGTGAAC 
ACCCAGGAGC TCCTGGACCT GCTGGTGAAG TGTGAGAACA AGATCCAGAC ACGGATTAAG 
ATTGGCCTCA ACTCCAAGAT GCCAAGTCGG TTCCCCCCTG TCGTGTTCTA CACGCCTAAG 
GAGTTGGGTG GACTTGGAAT GCTCTCAATG GGGCATGTGC TCATCCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTTC GTTCAGGAAT GAGCCATGAA 
GAAGACCAGT TGATTCCCAA CTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT 
GATTCTCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC 
AGACGACTGA CCTTAGAAGA TCTAGAAGAT TCATGGGATC GTGGTATTCC TCGAATCAAC 
ACCCTCTTCC AGAAGGACCG GCACACGCTG GCTTATGATA AGGGCTGGCG TGTTCGTACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAG AACCCTTTTT GGTGGACACA CCAGCGGCAT 
GACGGGAAGC TCTGGAACCT GAACAACTAC CGTACAGACA TGATCCAGGC CCTGGGTGGT 
GTGGAGGGCA TCCTGGAACA CACTCTCTTC AAGGGCACGT ACTTCCCTAC CTGGGAAGGG 
CTTTTCTGGG AGAAGGCCAG TGGTTTCGAG GAGTCTATGA AGTGGAAGAA GCTAACCAAC 
GCTCAGCGAT CTGGATTGAA CCAGATTCCC AATCGTAGAT TCACCCTCTG GTGGTCCCCC 
ACCATCAACC GAGCCAATGT GTACGTAGGC TTTCAGGTGC AGCTGGACCT GACGGGCATC 
TTCATGCATG GCAAGATCCC CACCCTGAAG ATCTCCCTCA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA 
CTGGATGCAC TGGAAATCGA GACGGTACAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT 
AAGATGAACT CTTCCTGTGC AGATATCCTG CTCTTTGCCT CCTACAAGTG GAATGTCTCC 
CGGCCCTCGT TGTTGGCAGA CTCCAAGGAT GTGATGGACA GCACCACCAC TCAGAAGTAT 
TGGATTGACA TCCAGTTGCG CTGGGGGGAC TATGATTCTC ATGACATTGA GCGCTACGCC 
CGGGCCAAGT TCTTAGACTA CACCACAGAC AATATGAGTA TCTACCCTTC ACCCACGGGC 
GTGCTCATTG CCATTGACCT GGCCTATAAC CTGCACAGTG CCTATGGAAA CTGGTTCCCT 
GGCAGCAAGC CTCTCATCCA GCAGGCCATG GCCAAAATCA TGAAGGCAAA CCCTGCCTTG 
TATGTGTTAC GGGAACGGAT CCGTAAGGGG TTGCAGCTCT ATTCTTCTGA GCCCACTGAG 
CCCTATCTCT CCTCTCAGAA CTATGGTGAA CTCTTCTCCA ACCAGATTAT CTGGTTTGTG 
GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACCT TCGAAGGGAA CTTGACCACC 
AAGCCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CGGGGCAGCT CTTCCTCAAG 
ATCATTCACA CGTCTGTGTG GGCTGGACAG AAGCGTCTGG GGCAGTTGGC TAAGTGGAAG 
ACGGCTGAGG AAGTGGCCGC CCTGATCCGC TCTTTGCCAG TGGAGGAGCA GCCCAAGCAG 
ATCATTGTCA CCAGGAAGGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC 
AATATCGTGA TCAAAGGCTC AGAGCTCCAG CTCCCCTTCC AGGCTTGTCT CAAGGTGGAA 
AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAACCGCAGA TGGTTCTCTT TAACCTCTAC 
GATGACTGGC TCAAGACCAT CTCATCTTAC ACGGCTTTCT CCCGCCTCAT CCTGATTCTG 
CGCGCCCTGC ATGTGAATAA CGACCGGGCG AAGGTGATCC TGAAGCCAGA CAAGACGACG 
ATCACGGAAC CACACCACAT CTGGCCCACT CTGACTGACG AGGAGTGGAT CAAGGTGGAG 
GTGCAGCTCA AGGACCTTAT CTTGGCCGAC TACGGCAAGA AAAACAATGT GAATGTGGCA 
TCACTGACAC AATCAGAAAT CCGAGACATC ATCCTGGGTA TGGAGATCTC AGCACCATCA 
CAGCAGCGGC AACAGATAGC TGAGATTGAG AAGCAGACCA AGGAACAGTC GCAGCTGACT 
GCGACCCAGA CTCGTACTGT CAACAAGCAC GGTGACGAGA TCATCACCTC TACCACCAGC 
AACTATGAGA CCCAGACTTT CTCATCCAAG ACAGAATGGA GGGTCAGGGC CATCTCTGCT 
GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGATAT CAAGGAGACT 
GGTTATACCT ACATCCTTCC CAAGAATGTG CTCAAGAAGT TTATCTGCAT ATCTGACCTT 
CGGGCCCAAA TTGCAGGGTA CCTGTATGGG GTGAGTCCAC CAGATAACCC CCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTGCCACAG TGGGGCACTC ACCAGACCGT GCATCTGCCT 
GGCCAGCTGC CCCAGCACGA GTACCTCAAG GAAATGGAAC CCTTAGGTTG GATTCATACA 
CAGCCCAACG AGTCCCCCCA GCTATCACCC CAGGATGTCA CCACCCATGC CAAGATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATCATCA TCACCTGCAG CTTCACACCA 
GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAGTG GGGCCGCCAG 
AACACAGACA AGGGCAACAA CCCCAAGGGC TACCTACCGT CGCACTACGA GAGGGTCCAG 
ATGCTACTGT CAGACCGCTT CCTTGGCTTC TTTATGGTCC CTGCCCAGTC CTCATGGAAC 
TACAACTTTA TGGGTGTCCG GCATGATCCC AACATGAAGT ATGAGCTGCA GCTGGCAAAC 
CCCAAAGAGT TCTACCACGA GGTGCACCGA CCTTCCCACT TCCTCAACTT CGCTCTCCTG 
CAGGAGGGTG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020923227.1
CDS	158..7165
Translation	MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	XM_021067568.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa pre-mRNA processing factor 8 (PRPF8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021067568.1

ORF Insert Sequence:

ATGGCCGGAG TGTTTCCTTA CCGAGGGCCG GGCAACCCGG TTCCCGGCCC TCTAGCTCCG 
CTGCCAGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CTCGAAAATG GCAGCAGTTG 
CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCTCA GAAGGAAGAC 
ATGCCTCCAG AACATGTTAG GAAGATCATT CGAGACCATG GGGACATGAC CAACAGGAAG 
TTTCGCCATG ACAAAAGGGT CTACCTGGGT GCTCTCAAGT ACATGCCCCA TGCAGTCCTC 
AAACTCCTGG AGAACATGCC GATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTATAC 
CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATTCCCT GGGTCATTGA GCCTGTCTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGAG AAAAAAGAGA CAGGAGGCAT 
TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CGCCTTTGGA CTATGCTGAC 
AACATCCTAG ATGTCGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGATCC TGAGGAGGAT 
GCCCCGGTGT TAGACTGGTT CTATGACCAC CAGCCGTTGA GGGACAGCCG AAAGTATGTG 
AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACACTGCCCA TGATGTCCAC TCTCTACCGC 
CTGGCCAATC AGCTTCTGAC AGACTTGGTA GATGACAACT ACTTCTATCT ATTTGATCTG 
AAGGCCTTTT TTACCTCCAA GGCACTCAAT ATGGCCATTC CTGGAGGCCC CAAGTTTGAG 
CCTCTTGTCC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGATATTAAC 
AAGATCATCA TCCGGCAGCC TATCCGCACT GAGTACAAGA TTGCCTTTCC TTACTTGTAC 
AACAACCTTC CACACCACGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACTGAGG ATCCTGACTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATATCC 
CACAGACACT CTGTCAAGAG CCAGGAACCG TTGCCGGATG ACGATGAAGA GTTTGAGCTC 
CCAGAGTTCG TGGAGCCCTT CCTGAAGGAC ACACCCCTCT ACACAGACAA CACAGCCAAT 
GGCATTGCCC TGCTCTGGGC CCCACGGCCC TTCAACCTAC GCTCTGGTCG CACCCGCCGG 
GCCCTAGACA TCCCCCTCGT CAAGAACTGG TATCGGGAGC ATTGTCCCGC TGGGCAGCCT 
GTGAAAGTGA GGGTCTCTTA CCAGAAGCTG CTTAAGTACT ATGTGCTGAA TGCCCTGAAG 
CACCGGCCCC CTAAAGCCCA AAAGAAGAGG TATCTGTTCC GTTCCTTCAA AGCCACCAAG 
TTCTTTCAGT CTACGAAGCT GGACTGGGTG GAGGTGGGGC TGCAGGTTTG CCGTCAGGGC 
TACAACATGC TCAACCTTCT TATTCACCGT AAAAATCTCA ACTATCTGCA TCTGGATTAC 
AACTTCAACC TCAAGCCCGT CAAGACGCTC ACCACCAAGG AAAGAAAGAA ATCTCGTTTT 
GGGAATGCTT TTCACCTGTG TCGAGAAGTT CTGCGTTTAA CTAAGCTGGT GGTGGACAGT 
CATGTGCAGT ATCGGTTGGG CAACGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAC 
ATCTTTGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGGT ACAAATACAA GCTGATGCGG 
CAGATAAGAA TGTGCAAGGA CCTGAAGCAT CTCATCTATT ACCGCTTCAA CACGGGCCCT 
GTGGGGAAGG GCCCTGGCTG TGGCTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTCTTC 
ATGCGTGGCA TCACCCCTTT GCTGGAGCGT TGGCTGGGCA ACCTCCTAGC TCGGCAGTTC 
GAAGGCCGGC ACTCAAAGGG GGTGGCAAAG ACTGTGACAA AGCAGCGCGT GGAATCGCAT 
TTTGACCTTG AGCTTCGGGC CGCTGTAATG CATGACATTC TGGACATGAT GCCGGAGGGA 
ATAAAACAGA ACAAGGCCCG GACCATCCTG CAGCACCTCA GTGAAGCCTG GCGCTGCTGG 
AAGGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCAACAC CTATAGAGAA TATGATCCTT 
CGATACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACTG CCCACTACAA CCGGGAACGA 
ATTCGCCGTG GGGCCACGGT AGATAAGACT GTTTGCAAAA AGAACCTGGG CCGCCTCACT 
CGGCTCTACC TGAAGGCAGA ACAGGAGCGG CAGCACAACT ACCTGAAGGA CGGGCCTTAC 
ATCACAGCAG AGGAAGCAGT GGCAGTATAT ACCACCACTG TGCATTGGTT GGAAAGCCGC 
AGGTTCTCAC CCATCCCCTT CCCCCCACTC TCCTACAAGC ATGACACCAA GTTGCTCATC 
TTAGCCTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CCCGGTTAAA CCAGTCTCAG 
AGGGAAGAGC TGGGTCTGAT CGAGCAAGCT TATGACAACC CCCATGAGGC GTTGTCCCGC 
ATCAAGCGTC ACCTTCTCAC ACAGAGAGCC TTCAAAGAAG TAGGCATTGA GTTCATGGAT 
CTCTATAGCC ACCTGGTGCC AGTGTATGAT GTGGAGCCCC TGGAGAAAAT CACTGATGCC 
TACCTGGACC AGTACCTCTG GTACGAAGCT GACAAGCGCC GCCTTTTCCC GCCCTGGATC 
AAGCCTGCTG ACACAGAACC GCCGCCTCTG CTTGTTTACA AGTGGTGCCA AGGCATCAAT 
AACCTGCAGG ACGTGTGGGA GACAAGCGAG GGCGAGTGCA ACGTCATGCT GGAGTCACGA 
TTTGAGAAGA TGTATGAGAA GATCGACCTG ACCCTGCTGA ACAGGCTGCT GCGCCTCATC 
GTAGACCACA ACATCGCTGA CTACATGACT GCCAAGAACA ACGTCGTCAT CAACTACAAG 
GACATGAACC ATACAAATTC GTATGGGATC ATCAGAGGCC TGCAGTTTGC CTCGTTCATC 
GTGCAGTACT ATGGCCTGGT GATGGATTTG CTTGTGTTGG GCTTGCACCG GGCCAGTGAG 
ATGGCTGGGC CCCCTCAGAT GCCAAACGAC TTTCTCAGTT TCCAAGACAT AGCTACTGAG 
GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATCGATC GTATCCATAT TTTTTTCAGG 
TTCACGGCAG ATGAGGCTCG GGACCTAATC CAGCGTTACC TGACGGAGCA TCCTGACCCC 
AACAATGAGA ACATCGTCGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA TGCCCGCATG 
CGCCTCATGA AGCATGATGT TAACTTGGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT 
CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCGGT GTACAGTAAG 
GACAACCCCA ACCTGCTGTT CAACATGTGC GGTTTTGAGT GCCGCATCCT GCCAAAATGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAATGAGGTT 
ACTAAGGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC 
CACAACCGTG TGCGTCAAAT TCTGATGGCC TCTGGGTCCA CCACCTTCAC CAAGATTGTG 
AATAAATGGA ACACAGCTCT TATTGGCCTT ATGACATACT TTCGGGAGGC TGTGGTGAAC 
ACCCAGGAGC TCCTGGACCT GCTGGTGAAG TGTGAGAACA AGATCCAGAC ACGGATTAAG 
ATTGGCCTCA ACTCCAAGAT GCCAAGTCGG TTCCCCCCTG TCGTGTTCTA CACGCCTAAG 
GAGTTGGGTG GACTTGGAAT GCTCTCAATG GGGCATGTGC TCATCCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTTC GTTCAGGAAT GAGCCATGAA 
GAAGACCAGT TGATTCCCAA CTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT 
GATTCTCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC 
AGACGACTGA CCTTAGAAGA TCTAGAAGAT TCATGGGATC GTGGTATTCC TCGAATCAAC 
ACCCTCTTCC AGAAGGACCG GCACACGCTG GCTTATGATA AGGGCTGGCG TGTTCGTACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAG AACCCTTTTT GGTGGACACA CCAGCGGCAT 
GACGGGAAGC TCTGGAACCT GAACAACTAC CGTACAGACA TGATCCAGGC CCTGGGTGGT 
GTGGAGGGCA TCCTGGAACA CACTCTCTTC AAGGGCACGT ACTTCCCTAC CTGGGAAGGG 
CTTTTCTGGG AGAAGGCCAG TGGTTTCGAG GAGTCTATGA AGTGGAAGAA GCTAACCAAC 
GCTCAGCGAT CTGGATTGAA CCAGATTCCC AATCGTAGAT TCACCCTCTG GTGGTCCCCC 
ACCATCAACC GAGCCAATGT GTACGTAGGC TTTCAGGTGC AGCTGGACCT GACGGGCATC 
TTCATGCATG GCAAGATCCC CACCCTGAAG ATCTCCCTCA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA 
CTGGATGCAC TGGAAATCGA GACGGTACAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT 
AAGATGAACT CTTCCTGTGC AGATATCCTG CTCTTTGCCT CCTACAAGTG GAATGTCTCC 
CGGCCCTCGT TGTTGGCAGA CTCCAAGGAT GTGATGGACA GCACCACCAC TCAGAAGTAT 
TGGATTGACA TCCAGTTGCG CTGGGGGGAC TATGATTCTC ATGACATTGA GCGCTACGCC 
CGGGCCAAGT TCTTAGACTA CACCACAGAC AATATGAGTA TCTACCCTTC ACCCACGGGC 
GTGCTCATTG CCATTGACCT GGCCTATAAC CTGCACAGTG CCTATGGAAA CTGGTTCCCT 
GGCAGCAAGC CTCTCATCCA GCAGGCCATG GCCAAAATCA TGAAGGCAAA CCCTGCCTTG 
TATGTGTTAC GGGAACGGAT CCGTAAGGGG TTGCAGCTCT ATTCTTCTGA GCCCACTGAG 
CCCTATCTCT CCTCTCAGAA CTATGGTGAA CTCTTCTCCA ACCAGATTAT CTGGTTTGTG 
GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACCT TCGAAGGGAA CTTGACCACC 
AAGCCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CGGGGCAGCT CTTCCTCAAG 
ATCATTCACA CGTCTGTGTG GGCTGGACAG AAGCGTCTGG GGCAGTTGGC TAAGTGGAAG 
ACGGCTGAGG AAGTGGCCGC CCTGATCCGC TCTTTGCCAG TGGAGGAGCA GCCCAAGCAG 
ATCATTGTCA CCAGGAAGGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC 
AATATCGTGA TCAAAGGCTC AGAGCTCCAG CTCCCCTTCC AGGCTTGTCT CAAGGTGGAA 
AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAACCGCAGA TGGTTCTCTT TAACCTCTAC 
GATGACTGGC TCAAGACCAT CTCATCTTAC ACGGCTTTCT CCCGCCTCAT CCTGATTCTG 
CGCGCCCTGC ATGTGAATAA CGACCGGGCG AAGGTGATCC TGAAGCCAGA CAAGACGACG 
ATCACGGAAC CACACCACAT CTGGCCCACT CTGACTGACG AGGAGTGGAT CAAGGTGGAG 
GTGCAGCTCA AGGACCTTAT CTTGGCCGAC TACGGCAAGA AAAACAATGT GAATGTGGCA 
TCACTGACAC AATCAGAAAT CCGAGACATC ATCCTGGGTA TGGAGATCTC AGCACCATCA 
CAGCAGCGGC AACAGATAGC TGAGATTGAG AAGCAGACCA AGGAACAGTC GCAGCTGACT 
GCGACCCAGA CTCGTACTGT CAACAAGCAC GGTGACGAGA TCATCACCTC TACCACCAGC 
AACTATGAGA CCCAGACTTT CTCATCCAAG ACAGAATGGA GGGTCAGGGC CATCTCTGCT 
GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGATAT CAAGGAGACT 
GGTTATACCT ACATCCTTCC CAAGAATGTG CTCAAGAAGT TTATCTGCAT ATCTGACCTT 
CGGGCCCAAA TTGCAGGGTA CCTGTATGGG GTGAGTCCAC CAGATAACCC CCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTGCCACAG TGGGGCACTC ACCAGACCGT GCATCTGCCT 
GGCCAGCTGC CCCAGCACGA GTACCTCAAG GAAATGGAAC CCTTAGGTTG GATTCATACA 
CAGCCCAACG AGTCCCCCCA GCTATCACCC CAGGATGTCA CCACCCATGC CAAGATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATCATCA TCACCTGCAG CTTCACACCA 
GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAGTG GGGCCGCCAG 
AACACAGACA AGGGCAACAA CCCCAAGGGC TACCTACCGT CGCACTACGA GAGGGTCCAG 
ATGCTACTGT CAGACCGCTT CCTTGGCTTC TTTATGGTCC CTGCCCAGTC CTCATGGAAC 
TACAACTTTA TGGGTGTCCG GCATGATCCC AACATGAAGT ATGAGCTGCA GCTGGCAAAC 
CCCAAAGAGT TCTACCACGA GGTGCACCGA CCTTCCCACT TCCTCAACTT CGCTCTCCTG 
CAGGAGGGTG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRPF8 ( XM_021067569.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_020923228.1 Sus scrofa pre-mRNA processing factor 8 (PRPF8), transcript variant X2, mRNA.

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CloneID	OSe234277
Clone ID Related Accession (Same CDS sequence)	XM_021067568.1 , XM_021067569.1
Accession Version	XM_021067569.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	pre-mRNA-processing-splicing factor 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010454.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGAG TGTTTCCTTA CCGAGGGCCG GGCAACCCGG TTCCCGGCCC TCTAGCTCCG 
CTGCCAGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CTCGAAAATG GCAGCAGTTG 
CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCTCA GAAGGAAGAC 
ATGCCTCCAG AACATGTTAG GAAGATCATT CGAGACCATG GGGACATGAC CAACAGGAAG 
TTTCGCCATG ACAAAAGGGT CTACCTGGGT GCTCTCAAGT ACATGCCCCA TGCAGTCCTC 
AAACTCCTGG AGAACATGCC GATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTATAC 
CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATTCCCT GGGTCATTGA GCCTGTCTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGAG AAAAAAGAGA CAGGAGGCAT 
TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CGCCTTTGGA CTATGCTGAC 
AACATCCTAG ATGTCGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGATCC TGAGGAGGAT 
GCCCCGGTGT TAGACTGGTT CTATGACCAC CAGCCGTTGA GGGACAGCCG AAAGTATGTG 
AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACACTGCCCA TGATGTCCAC TCTCTACCGC 
CTGGCCAATC AGCTTCTGAC AGACTTGGTA GATGACAACT ACTTCTATCT ATTTGATCTG 
AAGGCCTTTT TTACCTCCAA GGCACTCAAT ATGGCCATTC CTGGAGGCCC CAAGTTTGAG 
CCTCTTGTCC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGATATTAAC 
AAGATCATCA TCCGGCAGCC TATCCGCACT GAGTACAAGA TTGCCTTTCC TTACTTGTAC 
AACAACCTTC CACACCACGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACTGAGG ATCCTGACTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATATCC 
CACAGACACT CTGTCAAGAG CCAGGAACCG TTGCCGGATG ACGATGAAGA GTTTGAGCTC 
CCAGAGTTCG TGGAGCCCTT CCTGAAGGAC ACACCCCTCT ACACAGACAA CACAGCCAAT 
GGCATTGCCC TGCTCTGGGC CCCACGGCCC TTCAACCTAC GCTCTGGTCG CACCCGCCGG 
GCCCTAGACA TCCCCCTCGT CAAGAACTGG TATCGGGAGC ATTGTCCCGC TGGGCAGCCT 
GTGAAAGTGA GGGTCTCTTA CCAGAAGCTG CTTAAGTACT ATGTGCTGAA TGCCCTGAAG 
CACCGGCCCC CTAAAGCCCA AAAGAAGAGG TATCTGTTCC GTTCCTTCAA AGCCACCAAG 
TTCTTTCAGT CTACGAAGCT GGACTGGGTG GAGGTGGGGC TGCAGGTTTG CCGTCAGGGC 
TACAACATGC TCAACCTTCT TATTCACCGT AAAAATCTCA ACTATCTGCA TCTGGATTAC 
AACTTCAACC TCAAGCCCGT CAAGACGCTC ACCACCAAGG AAAGAAAGAA ATCTCGTTTT 
GGGAATGCTT TTCACCTGTG TCGAGAAGTT CTGCGTTTAA CTAAGCTGGT GGTGGACAGT 
CATGTGCAGT ATCGGTTGGG CAACGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAC 
ATCTTTGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGGT ACAAATACAA GCTGATGCGG 
CAGATAAGAA TGTGCAAGGA CCTGAAGCAT CTCATCTATT ACCGCTTCAA CACGGGCCCT 
GTGGGGAAGG GCCCTGGCTG TGGCTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTCTTC 
ATGCGTGGCA TCACCCCTTT GCTGGAGCGT TGGCTGGGCA ACCTCCTAGC TCGGCAGTTC 
GAAGGCCGGC ACTCAAAGGG GGTGGCAAAG ACTGTGACAA AGCAGCGCGT GGAATCGCAT 
TTTGACCTTG AGCTTCGGGC CGCTGTAATG CATGACATTC TGGACATGAT GCCGGAGGGA 
ATAAAACAGA ACAAGGCCCG GACCATCCTG CAGCACCTCA GTGAAGCCTG GCGCTGCTGG 
AAGGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCAACAC CTATAGAGAA TATGATCCTT 
CGATACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACTG CCCACTACAA CCGGGAACGA 
ATTCGCCGTG GGGCCACGGT AGATAAGACT GTTTGCAAAA AGAACCTGGG CCGCCTCACT 
CGGCTCTACC TGAAGGCAGA ACAGGAGCGG CAGCACAACT ACCTGAAGGA CGGGCCTTAC 
ATCACAGCAG AGGAAGCAGT GGCAGTATAT ACCACCACTG TGCATTGGTT GGAAAGCCGC 
AGGTTCTCAC CCATCCCCTT CCCCCCACTC TCCTACAAGC ATGACACCAA GTTGCTCATC 
TTAGCCTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CCCGGTTAAA CCAGTCTCAG 
AGGGAAGAGC TGGGTCTGAT CGAGCAAGCT TATGACAACC CCCATGAGGC GTTGTCCCGC 
ATCAAGCGTC ACCTTCTCAC ACAGAGAGCC TTCAAAGAAG TAGGCATTGA GTTCATGGAT 
CTCTATAGCC ACCTGGTGCC AGTGTATGAT GTGGAGCCCC TGGAGAAAAT CACTGATGCC 
TACCTGGACC AGTACCTCTG GTACGAAGCT GACAAGCGCC GCCTTTTCCC GCCCTGGATC 
AAGCCTGCTG ACACAGAACC GCCGCCTCTG CTTGTTTACA AGTGGTGCCA AGGCATCAAT 
AACCTGCAGG ACGTGTGGGA GACAAGCGAG GGCGAGTGCA ACGTCATGCT GGAGTCACGA 
TTTGAGAAGA TGTATGAGAA GATCGACCTG ACCCTGCTGA ACAGGCTGCT GCGCCTCATC 
GTAGACCACA ACATCGCTGA CTACATGACT GCCAAGAACA ACGTCGTCAT CAACTACAAG 
GACATGAACC ATACAAATTC GTATGGGATC ATCAGAGGCC TGCAGTTTGC CTCGTTCATC 
GTGCAGTACT ATGGCCTGGT GATGGATTTG CTTGTGTTGG GCTTGCACCG GGCCAGTGAG 
ATGGCTGGGC CCCCTCAGAT GCCAAACGAC TTTCTCAGTT TCCAAGACAT AGCTACTGAG 
GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATCGATC GTATCCATAT TTTTTTCAGG 
TTCACGGCAG ATGAGGCTCG GGACCTAATC CAGCGTTACC TGACGGAGCA TCCTGACCCC 
AACAATGAGA ACATCGTCGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA TGCCCGCATG 
CGCCTCATGA AGCATGATGT TAACTTGGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT 
CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCGGT GTACAGTAAG 
GACAACCCCA ACCTGCTGTT CAACATGTGC GGTTTTGAGT GCCGCATCCT GCCAAAATGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAATGAGGTT 
ACTAAGGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC 
CACAACCGTG TGCGTCAAAT TCTGATGGCC TCTGGGTCCA CCACCTTCAC CAAGATTGTG 
AATAAATGGA ACACAGCTCT TATTGGCCTT ATGACATACT TTCGGGAGGC TGTGGTGAAC 
ACCCAGGAGC TCCTGGACCT GCTGGTGAAG TGTGAGAACA AGATCCAGAC ACGGATTAAG 
ATTGGCCTCA ACTCCAAGAT GCCAAGTCGG TTCCCCCCTG TCGTGTTCTA CACGCCTAAG 
GAGTTGGGTG GACTTGGAAT GCTCTCAATG GGGCATGTGC TCATCCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTTC GTTCAGGAAT GAGCCATGAA 
GAAGACCAGT TGATTCCCAA CTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT 
GATTCTCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC 
AGACGACTGA CCTTAGAAGA TCTAGAAGAT TCATGGGATC GTGGTATTCC TCGAATCAAC 
ACCCTCTTCC AGAAGGACCG GCACACGCTG GCTTATGATA AGGGCTGGCG TGTTCGTACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAG AACCCTTTTT GGTGGACACA CCAGCGGCAT 
GACGGGAAGC TCTGGAACCT GAACAACTAC CGTACAGACA TGATCCAGGC CCTGGGTGGT 
GTGGAGGGCA TCCTGGAACA CACTCTCTTC AAGGGCACGT ACTTCCCTAC CTGGGAAGGG 
CTTTTCTGGG AGAAGGCCAG TGGTTTCGAG GAGTCTATGA AGTGGAAGAA GCTAACCAAC 
GCTCAGCGAT CTGGATTGAA CCAGATTCCC AATCGTAGAT TCACCCTCTG GTGGTCCCCC 
ACCATCAACC GAGCCAATGT GTACGTAGGC TTTCAGGTGC AGCTGGACCT GACGGGCATC 
TTCATGCATG GCAAGATCCC CACCCTGAAG ATCTCCCTCA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA 
CTGGATGCAC TGGAAATCGA GACGGTACAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT 
AAGATGAACT CTTCCTGTGC AGATATCCTG CTCTTTGCCT CCTACAAGTG GAATGTCTCC 
CGGCCCTCGT TGTTGGCAGA CTCCAAGGAT GTGATGGACA GCACCACCAC TCAGAAGTAT 
TGGATTGACA TCCAGTTGCG CTGGGGGGAC TATGATTCTC ATGACATTGA GCGCTACGCC 
CGGGCCAAGT TCTTAGACTA CACCACAGAC AATATGAGTA TCTACCCTTC ACCCACGGGC 
GTGCTCATTG CCATTGACCT GGCCTATAAC CTGCACAGTG CCTATGGAAA CTGGTTCCCT 
GGCAGCAAGC CTCTCATCCA GCAGGCCATG GCCAAAATCA TGAAGGCAAA CCCTGCCTTG 
TATGTGTTAC GGGAACGGAT CCGTAAGGGG TTGCAGCTCT ATTCTTCTGA GCCCACTGAG 
CCCTATCTCT CCTCTCAGAA CTATGGTGAA CTCTTCTCCA ACCAGATTAT CTGGTTTGTG 
GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACCT TCGAAGGGAA CTTGACCACC 
AAGCCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CGGGGCAGCT CTTCCTCAAG 
ATCATTCACA CGTCTGTGTG GGCTGGACAG AAGCGTCTGG GGCAGTTGGC TAAGTGGAAG 
ACGGCTGAGG AAGTGGCCGC CCTGATCCGC TCTTTGCCAG TGGAGGAGCA GCCCAAGCAG 
ATCATTGTCA CCAGGAAGGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC 
AATATCGTGA TCAAAGGCTC AGAGCTCCAG CTCCCCTTCC AGGCTTGTCT CAAGGTGGAA 
AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAACCGCAGA TGGTTCTCTT TAACCTCTAC 
GATGACTGGC TCAAGACCAT CTCATCTTAC ACGGCTTTCT CCCGCCTCAT CCTGATTCTG 
CGCGCCCTGC ATGTGAATAA CGACCGGGCG AAGGTGATCC TGAAGCCAGA CAAGACGACG 
ATCACGGAAC CACACCACAT CTGGCCCACT CTGACTGACG AGGAGTGGAT CAAGGTGGAG 
GTGCAGCTCA AGGACCTTAT CTTGGCCGAC TACGGCAAGA AAAACAATGT GAATGTGGCA 
TCACTGACAC AATCAGAAAT CCGAGACATC ATCCTGGGTA TGGAGATCTC AGCACCATCA 
CAGCAGCGGC AACAGATAGC TGAGATTGAG AAGCAGACCA AGGAACAGTC GCAGCTGACT 
GCGACCCAGA CTCGTACTGT CAACAAGCAC GGTGACGAGA TCATCACCTC TACCACCAGC 
AACTATGAGA CCCAGACTTT CTCATCCAAG ACAGAATGGA GGGTCAGGGC CATCTCTGCT 
GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGATAT CAAGGAGACT 
GGTTATACCT ACATCCTTCC CAAGAATGTG CTCAAGAAGT TTATCTGCAT ATCTGACCTT 
CGGGCCCAAA TTGCAGGGTA CCTGTATGGG GTGAGTCCAC CAGATAACCC CCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTGCCACAG TGGGGCACTC ACCAGACCGT GCATCTGCCT 
GGCCAGCTGC CCCAGCACGA GTACCTCAAG GAAATGGAAC CCTTAGGTTG GATTCATACA 
CAGCCCAACG AGTCCCCCCA GCTATCACCC CAGGATGTCA CCACCCATGC CAAGATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATCATCA TCACCTGCAG CTTCACACCA 
GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAGTG GGGCCGCCAG 
AACACAGACA AGGGCAACAA CCCCAAGGGC TACCTACCGT CGCACTACGA GAGGGTCCAG 
ATGCTACTGT CAGACCGCTT CCTTGGCTTC TTTATGGTCC CTGCCCAGTC CTCATGGAAC 
TACAACTTTA TGGGTGTCCG GCATGATCCC AACATGAAGT ATGAGCTGCA GCTGGCAAAC 
CCCAAAGAGT TCTACCACGA GGTGCACCGA CCTTCCCACT TCCTCAACTT CGCTCTCCTG 
CAGGAGGGTG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020923228.1
CDS	125..7132
Translation	MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	XM_021067569.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa pre-mRNA processing factor 8 (PRPF8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021067569.1

ORF Insert Sequence:

ATGGCCGGAG TGTTTCCTTA CCGAGGGCCG GGCAACCCGG TTCCCGGCCC TCTAGCTCCG 
CTGCCAGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CTCGAAAATG GCAGCAGTTG 
CAGGCCAAGC GCTATGCCGA AAAGCGGAAG TTTGGATTTG TGGATGCTCA GAAGGAAGAC 
ATGCCTCCAG AACATGTTAG GAAGATCATT CGAGACCATG GGGACATGAC CAACAGGAAG 
TTTCGCCATG ACAAAAGGGT CTACCTGGGT GCTCTCAAGT ACATGCCCCA TGCAGTCCTC 
AAACTCCTGG AGAACATGCC GATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTATAC 
CACATCACCG GAGCCATTTC CTTTGTCAAT GAGATTCCCT GGGTCATTGA GCCTGTCTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGTCGAG AAAAAAGAGA CAGGAGGCAT 
TTCAAGAGGA TGCGTTTCCC CCCTTTTGAT GATGAGGAGC CGCCTTTGGA CTATGCTGAC 
AACATCCTAG ATGTCGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGATCC TGAGGAGGAT 
GCCCCGGTGT TAGACTGGTT CTATGACCAC CAGCCGTTGA GGGACAGCCG AAAGTATGTG 
AATGGTTCCA CTTACCAGCG CTGGCAGTTC ACACTGCCCA TGATGTCCAC TCTCTACCGC 
CTGGCCAATC AGCTTCTGAC AGACTTGGTA GATGACAACT ACTTCTATCT ATTTGATCTG 
AAGGCCTTTT TTACCTCCAA GGCACTCAAT ATGGCCATTC CTGGAGGCCC CAAGTTTGAG 
CCTCTTGTCC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGATATTAAC 
AAGATCATCA TCCGGCAGCC TATCCGCACT GAGTACAAGA TTGCCTTTCC TTACTTGTAC 
AACAACCTTC CACACCACGT CCACCTCACC TGGTACCACA CTCCTAATGT TGTGTTCATC 
AAAACTGAGG ATCCTGACTT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATATCC 
CACAGACACT CTGTCAAGAG CCAGGAACCG TTGCCGGATG ACGATGAAGA GTTTGAGCTC 
CCAGAGTTCG TGGAGCCCTT CCTGAAGGAC ACACCCCTCT ACACAGACAA CACAGCCAAT 
GGCATTGCCC TGCTCTGGGC CCCACGGCCC TTCAACCTAC GCTCTGGTCG CACCCGCCGG 
GCCCTAGACA TCCCCCTCGT CAAGAACTGG TATCGGGAGC ATTGTCCCGC TGGGCAGCCT 
GTGAAAGTGA GGGTCTCTTA CCAGAAGCTG CTTAAGTACT ATGTGCTGAA TGCCCTGAAG 
CACCGGCCCC CTAAAGCCCA AAAGAAGAGG TATCTGTTCC GTTCCTTCAA AGCCACCAAG 
TTCTTTCAGT CTACGAAGCT GGACTGGGTG GAGGTGGGGC TGCAGGTTTG CCGTCAGGGC 
TACAACATGC TCAACCTTCT TATTCACCGT AAAAATCTCA ACTATCTGCA TCTGGATTAC 
AACTTCAACC TCAAGCCCGT CAAGACGCTC ACCACCAAGG AAAGAAAGAA ATCTCGTTTT 
GGGAATGCTT TTCACCTGTG TCGAGAAGTT CTGCGTTTAA CTAAGCTGGT GGTGGACAGT 
CATGTGCAGT ATCGGTTGGG CAACGTGGAT GCCTTCCAGC TGGCAGATGG GTTGCAGTAC 
ATCTTTGCCC ACGTGGGGCA GCTGACGGGC ATGTACCGGT ACAAATACAA GCTGATGCGG 
CAGATAAGAA TGTGCAAGGA CCTGAAGCAT CTCATCTATT ACCGCTTCAA CACGGGCCCT 
GTGGGGAAGG GCCCTGGCTG TGGCTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTCTTC 
ATGCGTGGCA TCACCCCTTT GCTGGAGCGT TGGCTGGGCA ACCTCCTAGC TCGGCAGTTC 
GAAGGCCGGC ACTCAAAGGG GGTGGCAAAG ACTGTGACAA AGCAGCGCGT GGAATCGCAT 
TTTGACCTTG AGCTTCGGGC CGCTGTAATG CATGACATTC TGGACATGAT GCCGGAGGGA 
ATAAAACAGA ACAAGGCCCG GACCATCCTG CAGCACCTCA GTGAAGCCTG GCGCTGCTGG 
AAGGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCAACAC CTATAGAGAA TATGATCCTT 
CGATACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACTG CCCACTACAA CCGGGAACGA 
ATTCGCCGTG GGGCCACGGT AGATAAGACT GTTTGCAAAA AGAACCTGGG CCGCCTCACT 
CGGCTCTACC TGAAGGCAGA ACAGGAGCGG CAGCACAACT ACCTGAAGGA CGGGCCTTAC 
ATCACAGCAG AGGAAGCAGT GGCAGTATAT ACCACCACTG TGCATTGGTT GGAAAGCCGC 
AGGTTCTCAC CCATCCCCTT CCCCCCACTC TCCTACAAGC ATGACACCAA GTTGCTCATC 
TTAGCCTTGG AGCGGCTCAA GGAAGCTTAC AGTGTGAAGT CCCGGTTAAA CCAGTCTCAG 
AGGGAAGAGC TGGGTCTGAT CGAGCAAGCT TATGACAACC CCCATGAGGC GTTGTCCCGC 
ATCAAGCGTC ACCTTCTCAC ACAGAGAGCC TTCAAAGAAG TAGGCATTGA GTTCATGGAT 
CTCTATAGCC ACCTGGTGCC AGTGTATGAT GTGGAGCCCC TGGAGAAAAT CACTGATGCC 
TACCTGGACC AGTACCTCTG GTACGAAGCT GACAAGCGCC GCCTTTTCCC GCCCTGGATC 
AAGCCTGCTG ACACAGAACC GCCGCCTCTG CTTGTTTACA AGTGGTGCCA AGGCATCAAT 
AACCTGCAGG ACGTGTGGGA GACAAGCGAG GGCGAGTGCA ACGTCATGCT GGAGTCACGA 
TTTGAGAAGA TGTATGAGAA GATCGACCTG ACCCTGCTGA ACAGGCTGCT GCGCCTCATC 
GTAGACCACA ACATCGCTGA CTACATGACT GCCAAGAACA ACGTCGTCAT CAACTACAAG 
GACATGAACC ATACAAATTC GTATGGGATC ATCAGAGGCC TGCAGTTTGC CTCGTTCATC 
GTGCAGTACT ATGGCCTGGT GATGGATTTG CTTGTGTTGG GCTTGCACCG GGCCAGTGAG 
ATGGCTGGGC CCCCTCAGAT GCCAAACGAC TTTCTCAGTT TCCAAGACAT AGCTACTGAG 
GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATCGATC GTATCCATAT TTTTTTCAGG 
TTCACGGCAG ATGAGGCTCG GGACCTAATC CAGCGTTACC TGACGGAGCA TCCTGACCCC 
AACAATGAGA ACATCGTCGG CTACAATAAC AAGAAGTGCT GGCCCCGAGA TGCCCGCATG 
CGCCTCATGA AGCATGATGT TAACTTGGGT CGGGCAGTGT TCTGGGACAT CAAGAACCGT 
CTGCCACGCT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCGGT GTACAGTAAG 
GACAACCCCA ACCTGCTGTT CAACATGTGC GGTTTTGAGT GCCGCATCCT GCCAAAATGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAATGAGGTT 
ACTAAGGAGC GGACAGCTCA GTGTTTCCTG CGTGTGGATG ATGAGTCAAT GCAGCGCTTC 
CACAACCGTG TGCGTCAAAT TCTGATGGCC TCTGGGTCCA CCACCTTCAC CAAGATTGTG 
AATAAATGGA ACACAGCTCT TATTGGCCTT ATGACATACT TTCGGGAGGC TGTGGTGAAC 
ACCCAGGAGC TCCTGGACCT GCTGGTGAAG TGTGAGAACA AGATCCAGAC ACGGATTAAG 
ATTGGCCTCA ACTCCAAGAT GCCAAGTCGG TTCCCCCCTG TCGTGTTCTA CACGCCTAAG 
GAGTTGGGTG GACTTGGAAT GCTCTCAATG GGGCATGTGC TCATCCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAGACAGA TGTAGGTATC ACACACTTTC GTTCAGGAAT GAGCCATGAA 
GAAGACCAGT TGATTCCCAA CTTGTATCGC TACATACAGC CATGGGAGAG TGAGTTCATT 
GATTCTCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCCAT TGCTCAGAAC 
AGACGACTGA CCTTAGAAGA TCTAGAAGAT TCATGGGATC GTGGTATTCC TCGAATCAAC 
ACCCTCTTCC AGAAGGACCG GCACACGCTG GCTTATGATA AGGGCTGGCG TGTTCGTACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAG AACCCTTTTT GGTGGACACA CCAGCGGCAT 
GACGGGAAGC TCTGGAACCT GAACAACTAC CGTACAGACA TGATCCAGGC CCTGGGTGGT 
GTGGAGGGCA TCCTGGAACA CACTCTCTTC AAGGGCACGT ACTTCCCTAC CTGGGAAGGG 
CTTTTCTGGG AGAAGGCCAG TGGTTTCGAG GAGTCTATGA AGTGGAAGAA GCTAACCAAC 
GCTCAGCGAT CTGGATTGAA CCAGATTCCC AATCGTAGAT TCACCCTCTG GTGGTCCCCC 
ACCATCAACC GAGCCAATGT GTACGTAGGC TTTCAGGTGC AGCTGGACCT GACGGGCATC 
TTCATGCATG GCAAGATCCC CACCCTGAAG ATCTCCCTCA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAGA AGATCCACGA GAGCATCGTT ATGGACTTGT GTCAGGTGTT TGATCAGGAA 
CTGGATGCAC TGGAAATCGA GACGGTACAG AAGGAGACAA TCCACCCCCG AAAGTCCTAT 
AAGATGAACT CTTCCTGTGC AGATATCCTG CTCTTTGCCT CCTACAAGTG GAATGTCTCC 
CGGCCCTCGT TGTTGGCAGA CTCCAAGGAT GTGATGGACA GCACCACCAC TCAGAAGTAT 
TGGATTGACA TCCAGTTGCG CTGGGGGGAC TATGATTCTC ATGACATTGA GCGCTACGCC 
CGGGCCAAGT TCTTAGACTA CACCACAGAC AATATGAGTA TCTACCCTTC ACCCACGGGC 
GTGCTCATTG CCATTGACCT GGCCTATAAC CTGCACAGTG CCTATGGAAA CTGGTTCCCT 
GGCAGCAAGC CTCTCATCCA GCAGGCCATG GCCAAAATCA TGAAGGCAAA CCCTGCCTTG 
TATGTGTTAC GGGAACGGAT CCGTAAGGGG TTGCAGCTCT ATTCTTCTGA GCCCACTGAG 
CCCTATCTCT CCTCTCAGAA CTATGGTGAA CTCTTCTCCA ACCAGATTAT CTGGTTTGTG 
GATGACACTA ATGTCTACAG AGTGACTATC CATAAGACCT TCGAAGGGAA CTTGACCACC 
AAGCCCATCA ATGGGGCCAT CTTCATCTTC AACCCACGCA CGGGGCAGCT CTTCCTCAAG 
ATCATTCACA CGTCTGTGTG GGCTGGACAG AAGCGTCTGG GGCAGTTGGC TAAGTGGAAG 
ACGGCTGAGG AAGTGGCCGC CCTGATCCGC TCTTTGCCAG TGGAGGAGCA GCCCAAGCAG 
ATCATTGTCA CCAGGAAGGG CATGCTGGAT CCACTGGAGG TGCACTTGCT GGACTTCCCC 
AATATCGTGA TCAAAGGCTC AGAGCTCCAG CTCCCCTTCC AGGCTTGTCT CAAGGTGGAA 
AAGTTTGGGG ATCTCATCCT TAAAGCCACT GAACCGCAGA TGGTTCTCTT TAACCTCTAC 
GATGACTGGC TCAAGACCAT CTCATCTTAC ACGGCTTTCT CCCGCCTCAT CCTGATTCTG 
CGCGCCCTGC ATGTGAATAA CGACCGGGCG AAGGTGATCC TGAAGCCAGA CAAGACGACG 
ATCACGGAAC CACACCACAT CTGGCCCACT CTGACTGACG AGGAGTGGAT CAAGGTGGAG 
GTGCAGCTCA AGGACCTTAT CTTGGCCGAC TACGGCAAGA AAAACAATGT GAATGTGGCA 
TCACTGACAC AATCAGAAAT CCGAGACATC ATCCTGGGTA TGGAGATCTC AGCACCATCA 
CAGCAGCGGC AACAGATAGC TGAGATTGAG AAGCAGACCA AGGAACAGTC GCAGCTGACT 
GCGACCCAGA CTCGTACTGT CAACAAGCAC GGTGACGAGA TCATCACCTC TACCACCAGC 
AACTATGAGA CCCAGACTTT CTCATCCAAG ACAGAATGGA GGGTCAGGGC CATCTCTGCT 
GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGATGATAT CAAGGAGACT 
GGTTATACCT ACATCCTTCC CAAGAATGTG CTCAAGAAGT TTATCTGCAT ATCTGACCTT 
CGGGCCCAAA TTGCAGGGTA CCTGTATGGG GTGAGTCCAC CAGATAACCC CCAGGTGAAG 
GAGATCCGCT GCATCGTGAT GGTGCCACAG TGGGGCACTC ACCAGACCGT GCATCTGCCT 
GGCCAGCTGC CCCAGCACGA GTACCTCAAG GAAATGGAAC CCTTAGGTTG GATTCATACA 
CAGCCCAACG AGTCCCCCCA GCTATCACCC CAGGATGTCA CCACCCATGC CAAGATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATCATCA TCACCTGCAG CTTCACACCA 
GGCTCCTGCA CCCTGACAGC CTACAAGCTG ACCCCCAGTG GCTATGAGTG GGGCCGCCAG 
AACACAGACA AGGGCAACAA CCCCAAGGGC TACCTACCGT CGCACTACGA GAGGGTCCAG 
ATGCTACTGT CAGACCGCTT CCTTGGCTTC TTTATGGTCC CTGCCCAGTC CTCATGGAAC 
TACAACTTTA TGGGTGTCCG GCATGATCCC AACATGAAGT ATGAGCTGCA GCTGGCAAAC 
CCCAAAGAGT TCTACCACGA GGTGCACCGA CCTTCCCACT TCCTCAACTT CGCTCTCCTG 
CAGGAGGGTG AGGTCTACTC TGCGGACCGA GAGGACCTCT ATGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRPF8 ( XM_003131826.4 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_003131874.4 Sus scrofa pre-mRNA processing factor 8 (PRPF8), mRNA.

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CloneID	OSe41344
Clone ID Related Accession (Same CDS sequence)	XM_003131826.4
Accession Version	XM_003131826.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	pre-mRNA-processing-splicing factor 8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003535906.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_003131826.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGATGC CTTGGGAGCA GATTCCGGAT GTGCCTGTGC TATACCACAT CACCGGAGCC 
ATTTCCTTTG TCAATGAGAT TCCCTGGGTC ATTGAGCCTG TCTACATCTC CCAGTGGGGG 
TCAATGTGGA TTATGATGCG TCGAGAAAAA AGAGACAGGA GGCATTTCAA GAGGATGCGT 
TTCCCCCCTT TTGATGATGA GGAGCCGCCT TTGGACTATG CTGACAACAT CCTAGATGTC 
GAGCCACTGG AAGCCATTCA GCTAGAGCTG GATCCTGAGG AGGATGCCCC GGTGTTAGAC 
TGGTTCTATG ACCACCAGCC GTTGAGGGAC AGCCGAAAGT ATGTGAATGG TTCCACTTAC 
CAGCGCTGGC AGTTCACACT GCCCATGATG TCCACTCTCT ACCGCCTGGC CAATCAGCTT 
CTGACAGACT TGGTAGATGA CAACTACTTC TATCTATTTG ATCTGAAGGC CTTTTTTACC 
TCCAAGGCAC TCAATATGGC CATTCCTGGA GGCCCCAAGT TTGAGCCTCT TGTCCGAGAC 
ATCAACCTAC AGGATGAAGA CTGGAATGAA TTCAATGATA TTAACAAGAT CATCATCCGG 
CAGCCTATCC GCACTGAGTA CAAGATTGCC TTTCCTTACT TGTACAACAA CCTTCCACAC 
CACGTCCACC TCACCTGGTA CCACACTCCT AATGTTGTGT TCATCAAAAC TGAGGATCCT 
GACTTGCCAG CTTTCTACTT TGATCCTTTG ATCAACCCAA TATCCCACAG ACACTCTGTC 
AAGAGCCAGG AACCGTTGCC GGATGACGAT GAAGAGTTTG AGCTCCCAGA GTTCGTGGAG 
CCCTTCCTGA AGGACACACC CCTCTACACA GACAACACAG CCAATGGCAT TGCCCTGCTC 
TGGGCCCCAC GGCCCTTCAA CCTACGCTCT GGTCGCACCC GCCGGGCCCT AGACATCCCC 
CTCGTCAAGA ACTGGTATCG GGAGCATTGT CCCGCTGGGC AGCCTGTGAA AGTGAGGGTC 
TCTTACCAGA AGCTGCTTAA GTACTATGTG CTGAATGCCC TGAAGCACCG GCCCCCTAAA 
GCCCAAAAGA AGAGGTATCT GTTCCGTTCC TTCAAAGCCA CCAAGTTCTT TCAGTCTACG 
AAGCTGGACT GGGTGGAGGT GGGGCTGCAG GTTTGCCGTC AGGGCTACAA CATGCTCAAC 
CTTCTTATTC ACCGTAAAAA TCTCAACTAT CTGCATCTGG ATTACAACTT CAACCTCAAG 
CCCGTCAAGA CGCTCACCAC CAAGGAAAGA AAGAAATCTC GTTTTGGGAA TGCTTTTCAC 
CTGTGTCGAG AAGTTCTGCG TTTAACTAAG CTGGTGGTGG ACAGTCATGT GCAGTATCGG 
TTGGGCAACG TGGATGCCTT CCAGCTGGCA GATGGGTTGC AGTACATCTT TGCCCACGTG 
GGGCAGCTGA CGGGCATGTA CCGGTACAAA TACAAGCTGA TGCGGCAGAT AAGAATGTGC 
AAGGACCTGA AGCATCTCAT CTATTACCGC TTCAACACGG GCCCTGTGGG GAAGGGCCCT 
GGCTGTGGCT TCTGGGCTGC TGGCTGGCGG GTCTGGCTGT TCTTCATGCG TGGCATCACC 
CCTTTGCTGG AGCGTTGGCT GGGCAACCTC CTAGCTCGGC AGTTCGAAGG CCGGCACTCA 
AAGGGGGTGG CAAAGACTGT GACAAAGCAG CGCGTGGAAT CGCATTTTGA CCTTGAGCTT 
CGGGCCGCTG TAATGCATGA CATTCTGGAC ATGATGCCGG AGGGAATAAA ACAGAACAAG 
GCCCGGACCA TCCTGCAGCA CCTCAGTGAA GCCTGGCGCT GCTGGAAGGC CAACATTCCC 
TGGAAGGTCC CTGGGCTGCC AACACCTATA GAGAATATGA TCCTTCGATA CGTGAAGGCC 
AAGGCTGACT GGTGGACCAA CACTGCCCAC TACAACCGGG AACGAATTCG CCGTGGGGCC 
ACGGTAGATA AGACTGTTTG CAAAAAGAAC CTGGGCCGCC TCACTCGGCT CTACCTGAAG 
GCAGAACAGG AGCGGCAGCA CAACTACCTG AAGGACGGGC CTTACATCAC AGCAGAGGAA 
GCAGTGGCAG TATATACCAC CACTGTGCAT TGGTTGGAAA GCCGCAGGTT CTCACCCATC 
CCCTTCCCCC CACTCTCCTA CAAGCATGAC ACCAAGTTGC TCATCTTAGC CTTGGAGCGG 
CTCAAGGAAG CTTACAGTGT GAAGTCCCGG TTAAACCAGT CTCAGAGGGA AGAGCTGGGT 
CTGATCGAGC AAGCTTATGA CAACCCCCAT GAGGCGTTGT CCCGCATCAA GCGTCACCTT 
CTCACACAGA GAGCCTTCAA AGAAGTAGGC ATTGAGTTCA TGGATCTCTA TAGCCACCTG 
GTGCCAGTGT ATGATGTGGA GCCCCTGGAG AAAATCACTG ATGCCTACCT GGACCAGTAC 
CTCTGGTACG AAGCTGACAA GCGCCGCCTT TTCCCGCCCT GGATCAAGCC TGCTGACACA 
GAACCGCCGC CTCTGCTTGT TTACAAGTGG TGCCAAGGCA TCAATAACCT GCAGGACGTG 
TGGGAGACAA GCGAGGGCGA GTGCAACGTC ATGCTGGAGT CACGATTTGA GAAGATGTAT 
GAGAAGATCG ACCTGACCCT GCTGAACAGG CTGCTGCGCC TCATCGTAGA CCACAACATC 
GCTGACTACA TGACTGCCAA GAACAACGTC GTCATCAACT ACAAGGACAT GAACCATACA 
AATTCGTATG GGATCATCAG AGGCCTGCAG TTTGCCTCGT TCATCGTGCA GTACTATGGC 
CTGGTGATGG ATTTGCTTGT GTTGGGCTTG CACCGGGCCA GTGAGATGGC TGGGCCCCCT 
CAGATGCCAA ACGACTTTCT CAGTTTCCAA GACATAGCTA CTGAGGCTGC CCACCCCATC 
CGTCTCTTCT GCAGATACAT CGATCGTATC CATATTTTTT TCAGGTTCAC GGCAGATGAG 
GCTCGGGACC TAATCCAGCG TTACCTGACG GAGCATCCTG ACCCCAACAA TGAGAACATC 
GTCGGCTACA ATAACAAGAA GTGCTGGCCC CGAGATGCCC GCATGCGCCT CATGAAGCAT 
GATGTTAACT TGGGTCGGGC AGTGTTCTGG GACATCAAGA ACCGTCTGCC ACGCTCAGTG 
ACTACAGTTC AGTGGGAGAA CAGCTTCGTG TCGGTGTACA GTAAGGACAA CCCCAACCTG 
CTGTTCAACA TGTGCGGTTT TGAGTGCCGC ATCCTGCCAA AATGCCGCAC CAGCTATGAG 
GAGTTCACCC ACAAGGACGG GGTCTGGAAC CTACAGAATG AGGTTACTAA GGAGCGGACA 
GCTCAGTGTT TCCTGCGTGT GGATGATGAG TCAATGCAGC GCTTCCACAA CCGTGTGCGT 
CAAATTCTGA TGGCCTCTGG GTCCACCACC TTCACCAAGA TTGTGAATAA ATGGAACACA 
GCTCTTATTG GCCTTATGAC ATACTTTCGG GAGGCTGTGG TGAACACCCA GGAGCTCCTG 
GACCTGCTGG TGAAGTGTGA GAACAAGATC CAGACACGGA TTAAGATTGG CCTCAACTCC 
AAGATGCCAA GTCGGTTCCC CCCTGTCGTG TTCTACACGC CTAAGGAGTT GGGTGGACTT 
GGAATGCTCT CAATGGGGCA TGTGCTCATC CCCCAGTCTG ACCTCAGGTG GTCCAAGCAG 
ACAGATGTAG GTATCACACA CTTTCGTTCA GGAATGAGCC ATGAAGAAGA CCAGTTGATT 
CCCAACTTGT ATCGCTACAT ACAGCCATGG GAGAGTGAGT TCATTGATTC TCAGCGGGTC 
TGGGCTGAGT ATGCACTCAA GAGACAAGAG GCCATTGCTC AGAACAGACG ACTGACCTTA 
GAAGATCTAG AAGATTCATG GGATCGTGGT ATTCCTCGAA TCAACACCCT CTTCCAGAAG 
GACCGGCACA CGCTGGCTTA TGATAAGGGC TGGCGTGTTC GTACTGACTT TAAGCAGTAC 
CAGGTTTTGA AGCAGAACCC TTTTTGGTGG ACACACCAGC GGCATGACGG GAAGCTCTGG 
AACCTGAACA ACTACCGTAC AGACATGATC CAGGCCCTGG GTGGTGTGGA GGGCATCCTG 
GAACACACTC TCTTCAAGGG CACGTACTTC CCTACCTGGG AAGGGCTTTT CTGGGAGAAG 
GCCAGTGGTT TCGAGGAGTC TATGAAGTGG AAGAAGCTAA CCAACGCTCA GCGATCTGGA 
TTGAACCAGA TTCCCAATCG TAGATTCACC CTCTGGTGGT CCCCCACCAT CAACCGAGCC 
AATGTGTACG TAGGCTTTCA GGTGCAGCTG GACCTGACGG GCATCTTCAT GCATGGCAAG 
ATCCCCACCC TGAAGATCTC CCTCATCCAG ATCTTCCGAG CTCACTTGTG GCAGAAGATC 
CACGAGAGCA TCGTTATGGA CTTGTGTCAG GTGTTTGATC AGGAACTGGA TGCACTGGAA 
ATCGAGACGG TACAGAAGGA GACAATCCAC CCCCGAAAGT CCTATAAGAT GAACTCTTCC 
TGTGCAGATA TCCTGCTCTT TGCCTCCTAC AAGTGGAATG TCTCCCGGCC CTCGTTGTTG 
GCAGACTCCA AGGATGTGAT GGACAGCACC ACCACTCAGA AGTATTGGAT TGACATCCAG 
TTGCGCTGGG GGGACTATGA TTCTCATGAC ATTGAGCGCT ACGCCCGGGC CAAGTTCTTA 
GACTACACCA CAGACAATAT GAGTATCTAC CCTTCACCCA CGGGCGTGCT CATTGCCATT 
GACCTGGCCT ATAACCTGCA CAGTGCCTAT GGAAACTGGT TCCCTGGCAG CAAGCCTCTC 
ATCCAGCAGG CCATGGCCAA AATCATGAAG GCAAACCCTG CCTTGTATGT GTTACGGGAA 
CGGATCCGTA AGGGGTTGCA GCTCTATTCT TCTGAGCCCA CTGAGCCCTA TCTCTCCTCT 
CAGAACTATG GTGAACTCTT CTCCAACCAG ATTATCTGGT TTGTGGATGA CACTAATGTC 
TACAGAGTGA CTATCCATAA GACCTTCGAA GGGAACTTGA CCACCAAGCC CATCAATGGG 
GCCATCTTCA TCTTCAACCC ACGCACGGGG CAGCTCTTCC TCAAGATCAT TCACACGTCT 
GTGTGGGCTG GACAGAAGCG TCTGGGGCAG TTGGCTAAGT GGAAGACGGC TGAGGAAGTG 
GCCGCCCTGA TCCGCTCTTT GCCAGTGGAG GAGCAGCCCA AGCAGATCAT TGTCACCAGG 
AAGGGCATGC TGGATCCACT GGAGGTGCAC TTGCTGGACT TCCCCAATAT CGTGATCAAA 
GGCTCAGAGC TCCAGCTCCC CTTCCAGGCT TGTCTCAAGG TGGAAAAGTT TGGGGATCTC 
ATCCTTAAAG CCACTGAACC GCAGATGGTT CTCTTTAACC TCTACGATGA CTGGCTCAAG 
ACCATCTCAT CTTACACGGC TTTCTCCCGC CTCATCCTGA TTCTGCGCGC CCTGCATGTG 
AATAACGACC GGGCGAAGGT GATCCTGAAG CCAGACAAGA CGACGATCAC GGAACCACAC 
CACATCTGGC CCACTCTGAC TGACGAGGAG TGGATCAAGG TGGAGGTGCA GCTCAAGGAC 
CTTATCTTGG CCGACTACGG CAAGAAAAAC AATGTGAATG TGGCATCACT GACACAATCA 
GAAATCCGAG ACATCATCCT GGGTATGGAG ATCTCAGCAC CATCACAGCA GCGGCAACAG 
ATAGCTGAGA TTGAGAAGCA GACCAAGGAA CAGTCGCAGC TGACTGCGAC CCAGACTCGT 
ACTGTCAACA AGCACGGTGA CGAGATCATC ACCTCTACCA CCAGCAACTA TGAGACCCAG 
ACTTTCTCAT CCAAGACAGA ATGGAGGGTC AGGGCCATCT CTGCTGCCAA CCTGCACCTA 
AGGACCAATC ACATCTATGT TTCATCTGAT GATATCAAGG AGACTGGTTA TACCTACATC 
CTTCCCAAGA ATGTGCTCAA GAAGTTTATC TGCATATCTG ACCTTCGGGC CCAAATTGCA 
GGGTACCTGT ATGGGGTGAG TCCACCAGAT AACCCCCAGG TGAAGGAGAT CCGCTGCATC 
GTGATGGTGC CACAGTGGGG CACTCACCAG ACCGTGCATC TGCCTGGCCA GCTGCCCCAG 
CACGAGTACC TCAAGGAAAT GGAACCCTTA GGTTGGATTC ATACACAGCC CAACGAGTCC 
CCCCAGCTAT CACCCCAGGA TGTCACCACC CATGCCAAGA TCATGGCTGA CAACCCATCT 
TGGGATGGCG AGAAGACCAT CATCATCACC TGCAGCTTCA CACCAGGCTC CTGCACCCTG 
ACAGCCTACA AGCTGACCCC CAGTGGCTAT GAGTGGGGCC GCCAGAACAC AGACAAGGGC 
AACAACCCCA AGGGCTACCT ACCGTCGCAC TACGAGAGGG TCCAGATGCT ACTGTCAGAC 
CGCTTCCTTG GCTTCTTTAT GGTCCCTGCC CAGTCCTCAT GGAACTACAA CTTTATGGGT 
GTCCGGCATG ATCCCAACAT GAAGTATGAG CTGCAGCTGG CAAACCCCAA AGAGTTCTAC 
CACGAGGTGC ACCGACCTTC CCACTTCCTC AACTTCGCTC TCCTGCAGGA GGGTGAGGTC 
TACTCTGCGG ACCGAGAGGA CCTCTATGCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003131874.4
CDS	8..6700
Translation	MPMPWEQIPDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	XM_003131826.4
Organism	Sus scrofa(Pig)
Definition	Sus scrofa pre-mRNA processing factor 8 (PRPF8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003131826.4

ORF Insert Sequence:

ATGCCGATGC CTTGGGAGCA GATTCCGGAT GTGCCTGTGC TATACCACAT CACCGGAGCC 
ATTTCCTTTG TCAATGAGAT TCCCTGGGTC ATTGAGCCTG TCTACATCTC CCAGTGGGGG 
TCAATGTGGA TTATGATGCG TCGAGAAAAA AGAGACAGGA GGCATTTCAA GAGGATGCGT 
TTCCCCCCTT TTGATGATGA GGAGCCGCCT TTGGACTATG CTGACAACAT CCTAGATGTC 
GAGCCACTGG AAGCCATTCA GCTAGAGCTG GATCCTGAGG AGGATGCCCC GGTGTTAGAC 
TGGTTCTATG ACCACCAGCC GTTGAGGGAC AGCCGAAAGT ATGTGAATGG TTCCACTTAC 
CAGCGCTGGC AGTTCACACT GCCCATGATG TCCACTCTCT ACCGCCTGGC CAATCAGCTT 
CTGACAGACT TGGTAGATGA CAACTACTTC TATCTATTTG ATCTGAAGGC CTTTTTTACC 
TCCAAGGCAC TCAATATGGC CATTCCTGGA GGCCCCAAGT TTGAGCCTCT TGTCCGAGAC 
ATCAACCTAC AGGATGAAGA CTGGAATGAA TTCAATGATA TTAACAAGAT CATCATCCGG 
CAGCCTATCC GCACTGAGTA CAAGATTGCC TTTCCTTACT TGTACAACAA CCTTCCACAC 
CACGTCCACC TCACCTGGTA CCACACTCCT AATGTTGTGT TCATCAAAAC TGAGGATCCT 
GACTTGCCAG CTTTCTACTT TGATCCTTTG ATCAACCCAA TATCCCACAG ACACTCTGTC 
AAGAGCCAGG AACCGTTGCC GGATGACGAT GAAGAGTTTG AGCTCCCAGA GTTCGTGGAG 
CCCTTCCTGA AGGACACACC CCTCTACACA GACAACACAG CCAATGGCAT TGCCCTGCTC 
TGGGCCCCAC GGCCCTTCAA CCTACGCTCT GGTCGCACCC GCCGGGCCCT AGACATCCCC 
CTCGTCAAGA ACTGGTATCG GGAGCATTGT CCCGCTGGGC AGCCTGTGAA AGTGAGGGTC 
TCTTACCAGA AGCTGCTTAA GTACTATGTG CTGAATGCCC TGAAGCACCG GCCCCCTAAA 
GCCCAAAAGA AGAGGTATCT GTTCCGTTCC TTCAAAGCCA CCAAGTTCTT TCAGTCTACG 
AAGCTGGACT GGGTGGAGGT GGGGCTGCAG GTTTGCCGTC AGGGCTACAA CATGCTCAAC 
CTTCTTATTC ACCGTAAAAA TCTCAACTAT CTGCATCTGG ATTACAACTT CAACCTCAAG 
CCCGTCAAGA CGCTCACCAC CAAGGAAAGA AAGAAATCTC GTTTTGGGAA TGCTTTTCAC 
CTGTGTCGAG AAGTTCTGCG TTTAACTAAG CTGGTGGTGG ACAGTCATGT GCAGTATCGG 
TTGGGCAACG TGGATGCCTT CCAGCTGGCA GATGGGTTGC AGTACATCTT TGCCCACGTG 
GGGCAGCTGA CGGGCATGTA CCGGTACAAA TACAAGCTGA TGCGGCAGAT AAGAATGTGC 
AAGGACCTGA AGCATCTCAT CTATTACCGC TTCAACACGG GCCCTGTGGG GAAGGGCCCT 
GGCTGTGGCT TCTGGGCTGC TGGCTGGCGG GTCTGGCTGT TCTTCATGCG TGGCATCACC 
CCTTTGCTGG AGCGTTGGCT GGGCAACCTC CTAGCTCGGC AGTTCGAAGG CCGGCACTCA 
AAGGGGGTGG CAAAGACTGT GACAAAGCAG CGCGTGGAAT CGCATTTTGA CCTTGAGCTT 
CGGGCCGCTG TAATGCATGA CATTCTGGAC ATGATGCCGG AGGGAATAAA ACAGAACAAG 
GCCCGGACCA TCCTGCAGCA CCTCAGTGAA GCCTGGCGCT GCTGGAAGGC CAACATTCCC 
TGGAAGGTCC CTGGGCTGCC AACACCTATA GAGAATATGA TCCTTCGATA CGTGAAGGCC 
AAGGCTGACT GGTGGACCAA CACTGCCCAC TACAACCGGG AACGAATTCG CCGTGGGGCC 
ACGGTAGATA AGACTGTTTG CAAAAAGAAC CTGGGCCGCC TCACTCGGCT CTACCTGAAG 
GCAGAACAGG AGCGGCAGCA CAACTACCTG AAGGACGGGC CTTACATCAC AGCAGAGGAA 
GCAGTGGCAG TATATACCAC CACTGTGCAT TGGTTGGAAA GCCGCAGGTT CTCACCCATC 
CCCTTCCCCC CACTCTCCTA CAAGCATGAC ACCAAGTTGC TCATCTTAGC CTTGGAGCGG 
CTCAAGGAAG CTTACAGTGT GAAGTCCCGG TTAAACCAGT CTCAGAGGGA AGAGCTGGGT 
CTGATCGAGC AAGCTTATGA CAACCCCCAT GAGGCGTTGT CCCGCATCAA GCGTCACCTT 
CTCACACAGA GAGCCTTCAA AGAAGTAGGC ATTGAGTTCA TGGATCTCTA TAGCCACCTG 
GTGCCAGTGT ATGATGTGGA GCCCCTGGAG AAAATCACTG ATGCCTACCT GGACCAGTAC 
CTCTGGTACG AAGCTGACAA GCGCCGCCTT TTCCCGCCCT GGATCAAGCC TGCTGACACA 
GAACCGCCGC CTCTGCTTGT TTACAAGTGG TGCCAAGGCA TCAATAACCT GCAGGACGTG 
TGGGAGACAA GCGAGGGCGA GTGCAACGTC ATGCTGGAGT CACGATTTGA GAAGATGTAT 
GAGAAGATCG ACCTGACCCT GCTGAACAGG CTGCTGCGCC TCATCGTAGA CCACAACATC 
GCTGACTACA TGACTGCCAA GAACAACGTC GTCATCAACT ACAAGGACAT GAACCATACA 
AATTCGTATG GGATCATCAG AGGCCTGCAG TTTGCCTCGT TCATCGTGCA GTACTATGGC 
CTGGTGATGG ATTTGCTTGT GTTGGGCTTG CACCGGGCCA GTGAGATGGC TGGGCCCCCT 
CAGATGCCAA ACGACTTTCT CAGTTTCCAA GACATAGCTA CTGAGGCTGC CCACCCCATC 
CGTCTCTTCT GCAGATACAT CGATCGTATC CATATTTTTT TCAGGTTCAC GGCAGATGAG 
GCTCGGGACC TAATCCAGCG TTACCTGACG GAGCATCCTG ACCCCAACAA TGAGAACATC 
GTCGGCTACA ATAACAAGAA GTGCTGGCCC CGAGATGCCC GCATGCGCCT CATGAAGCAT 
GATGTTAACT TGGGTCGGGC AGTGTTCTGG GACATCAAGA ACCGTCTGCC ACGCTCAGTG 
ACTACAGTTC AGTGGGAGAA CAGCTTCGTG TCGGTGTACA GTAAGGACAA CCCCAACCTG 
CTGTTCAACA TGTGCGGTTT TGAGTGCCGC ATCCTGCCAA AATGCCGCAC CAGCTATGAG 
GAGTTCACCC ACAAGGACGG GGTCTGGAAC CTACAGAATG AGGTTACTAA GGAGCGGACA 
GCTCAGTGTT TCCTGCGTGT GGATGATGAG TCAATGCAGC GCTTCCACAA CCGTGTGCGT 
CAAATTCTGA TGGCCTCTGG GTCCACCACC TTCACCAAGA TTGTGAATAA ATGGAACACA 
GCTCTTATTG GCCTTATGAC ATACTTTCGG GAGGCTGTGG TGAACACCCA GGAGCTCCTG 
GACCTGCTGG TGAAGTGTGA GAACAAGATC CAGACACGGA TTAAGATTGG CCTCAACTCC 
AAGATGCCAA GTCGGTTCCC CCCTGTCGTG TTCTACACGC CTAAGGAGTT GGGTGGACTT 
GGAATGCTCT CAATGGGGCA TGTGCTCATC CCCCAGTCTG ACCTCAGGTG GTCCAAGCAG 
ACAGATGTAG GTATCACACA CTTTCGTTCA GGAATGAGCC ATGAAGAAGA CCAGTTGATT 
CCCAACTTGT ATCGCTACAT ACAGCCATGG GAGAGTGAGT TCATTGATTC TCAGCGGGTC 
TGGGCTGAGT ATGCACTCAA GAGACAAGAG GCCATTGCTC AGAACAGACG ACTGACCTTA 
GAAGATCTAG AAGATTCATG GGATCGTGGT ATTCCTCGAA TCAACACCCT CTTCCAGAAG 
GACCGGCACA CGCTGGCTTA TGATAAGGGC TGGCGTGTTC GTACTGACTT TAAGCAGTAC 
CAGGTTTTGA AGCAGAACCC TTTTTGGTGG ACACACCAGC GGCATGACGG GAAGCTCTGG 
AACCTGAACA ACTACCGTAC AGACATGATC CAGGCCCTGG GTGGTGTGGA GGGCATCCTG 
GAACACACTC TCTTCAAGGG CACGTACTTC CCTACCTGGG AAGGGCTTTT CTGGGAGAAG 
GCCAGTGGTT TCGAGGAGTC TATGAAGTGG AAGAAGCTAA CCAACGCTCA GCGATCTGGA 
TTGAACCAGA TTCCCAATCG TAGATTCACC CTCTGGTGGT CCCCCACCAT CAACCGAGCC 
AATGTGTACG TAGGCTTTCA GGTGCAGCTG GACCTGACGG GCATCTTCAT GCATGGCAAG 
ATCCCCACCC TGAAGATCTC CCTCATCCAG ATCTTCCGAG CTCACTTGTG GCAGAAGATC 
CACGAGAGCA TCGTTATGGA CTTGTGTCAG GTGTTTGATC AGGAACTGGA TGCACTGGAA 
ATCGAGACGG TACAGAAGGA GACAATCCAC CCCCGAAAGT CCTATAAGAT GAACTCTTCC 
TGTGCAGATA TCCTGCTCTT TGCCTCCTAC AAGTGGAATG TCTCCCGGCC CTCGTTGTTG 
GCAGACTCCA AGGATGTGAT GGACAGCACC ACCACTCAGA AGTATTGGAT TGACATCCAG 
TTGCGCTGGG GGGACTATGA TTCTCATGAC ATTGAGCGCT ACGCCCGGGC CAAGTTCTTA 
GACTACACCA CAGACAATAT GAGTATCTAC CCTTCACCCA CGGGCGTGCT CATTGCCATT 
GACCTGGCCT ATAACCTGCA CAGTGCCTAT GGAAACTGGT TCCCTGGCAG CAAGCCTCTC 
ATCCAGCAGG CCATGGCCAA AATCATGAAG GCAAACCCTG CCTTGTATGT GTTACGGGAA 
CGGATCCGTA AGGGGTTGCA GCTCTATTCT TCTGAGCCCA CTGAGCCCTA TCTCTCCTCT 
CAGAACTATG GTGAACTCTT CTCCAACCAG ATTATCTGGT TTGTGGATGA CACTAATGTC 
TACAGAGTGA CTATCCATAA GACCTTCGAA GGGAACTTGA CCACCAAGCC CATCAATGGG 
GCCATCTTCA TCTTCAACCC ACGCACGGGG CAGCTCTTCC TCAAGATCAT TCACACGTCT 
GTGTGGGCTG GACAGAAGCG TCTGGGGCAG TTGGCTAAGT GGAAGACGGC TGAGGAAGTG 
GCCGCCCTGA TCCGCTCTTT GCCAGTGGAG GAGCAGCCCA AGCAGATCAT TGTCACCAGG 
AAGGGCATGC TGGATCCACT GGAGGTGCAC TTGCTGGACT TCCCCAATAT CGTGATCAAA 
GGCTCAGAGC TCCAGCTCCC CTTCCAGGCT TGTCTCAAGG TGGAAAAGTT TGGGGATCTC 
ATCCTTAAAG CCACTGAACC GCAGATGGTT CTCTTTAACC TCTACGATGA CTGGCTCAAG 
ACCATCTCAT CTTACACGGC TTTCTCCCGC CTCATCCTGA TTCTGCGCGC CCTGCATGTG 
AATAACGACC GGGCGAAGGT GATCCTGAAG CCAGACAAGA CGACGATCAC GGAACCACAC 
CACATCTGGC CCACTCTGAC TGACGAGGAG TGGATCAAGG TGGAGGTGCA GCTCAAGGAC 
CTTATCTTGG CCGACTACGG CAAGAAAAAC AATGTGAATG TGGCATCACT GACACAATCA 
GAAATCCGAG ACATCATCCT GGGTATGGAG ATCTCAGCAC CATCACAGCA GCGGCAACAG 
ATAGCTGAGA TTGAGAAGCA GACCAAGGAA CAGTCGCAGC TGACTGCGAC CCAGACTCGT 
ACTGTCAACA AGCACGGTGA CGAGATCATC ACCTCTACCA CCAGCAACTA TGAGACCCAG 
ACTTTCTCAT CCAAGACAGA ATGGAGGGTC AGGGCCATCT CTGCTGCCAA CCTGCACCTA 
AGGACCAATC ACATCTATGT TTCATCTGAT GATATCAAGG AGACTGGTTA TACCTACATC 
CTTCCCAAGA ATGTGCTCAA GAAGTTTATC TGCATATCTG ACCTTCGGGC CCAAATTGCA 
GGGTACCTGT ATGGGGTGAG TCCACCAGAT AACCCCCAGG TGAAGGAGAT CCGCTGCATC 
GTGATGGTGC CACAGTGGGG CACTCACCAG ACCGTGCATC TGCCTGGCCA GCTGCCCCAG 
CACGAGTACC TCAAGGAAAT GGAACCCTTA GGTTGGATTC ATACACAGCC CAACGAGTCC 
CCCCAGCTAT CACCCCAGGA TGTCACCACC CATGCCAAGA TCATGGCTGA CAACCCATCT 
TGGGATGGCG AGAAGACCAT CATCATCACC TGCAGCTTCA CACCAGGCTC CTGCACCCTG 
ACAGCCTACA AGCTGACCCC CAGTGGCTAT GAGTGGGGCC GCCAGAACAC AGACAAGGGC 
AACAACCCCA AGGGCTACCT ACCGTCGCAC TACGAGAGGG TCCAGATGCT ACTGTCAGAC 
CGCTTCCTTG GCTTCTTTAT GGTCCCTGCC CAGTCCTCAT GGAACTACAA CTTTATGGGT 
GTCCGGCATG ATCCCAACAT GAAGTATGAG CTGCAGCTGG CAAACCCCAA AGAGTTCTAC 
CACGAGGTGC ACCGACCTTC CCACTTCCTC AACTTCGCTC TCCTGCAGGA GGGTGAGGTC 
TACTCTGCGG ACCGAGAGGA CCTCTATGCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PRPF8 cDNA ORF clone, Sus scrofa(Pig)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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