COL6A6 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL6A6
Entrez Gene ID	100920576
Full Name	collagen type VI alpha 6 chain
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012551346.1	XP_012406800.1	collagen alpha-6(VI) chain
XM_023505163.1	XP_023360931.1	collagen alpha-6(VI) chain
XM_023505163.2	XP_023360931.1	collagen alpha-6(VI) chain

Pathways from BioSystems
Gene Ontology

The following COL6A6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL6A6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa134300	XM_012551346.1 Latest version!	Sarcophilus harrisii collagen, type VI, alpha 6 (COL6A6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa156540	XM_023505163.2 Latest version!	Sarcophilus harrisii collagen type VI alpha 6 chain (COL6A6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OSa156540	XM_023505163.1	Sarcophilus harrisii collagen type VI alpha 6 chain (COL6A6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL6A6 ( XM_012551346.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012406800.1 Sarcophilus harrisii collagen, type VI, alpha 6 (COL6A6), mRNA.

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CloneID	OSa134300
Clone ID Related Accession (Same CDS sequence)	XM_012551346.1
Accession Version	XM_012551346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	collagen alpha-6(VI) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003843704.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

ATGGTGCGTC TCGGAGATCT CAGTTCCCTG GAAGCGCGGC CGGAGCCGCT GGGCGCCCGA 
GAGAGCTTGG GCCCGGTTCC CCGCCTCTGT GTGGAGGCTC CAGCTGCGGG GGCCGGGTGG 
GGTGATTACA GCCCTAGGGC TTCCACCGCC CCTGCACCTC TGGCTGCGCT CCACGCGCAG 
TTCTGGCTCC AGGGTCCTGA GTTTGCTGAT GTTGTGTTCC TGGTGGATAG CTCTGATCAC 
CTAGCGAGTA AGTTCTCTTT TGTGAGAACC TTCATCTCCA AGACGATCAA CAGTCTGCCC 
ATTGAAGCCA ACAAGTTCCG TATTGCCCTC GCCCAGTATA GCGATGATCT GCGCCAGGAG 
TTCCTGCTGA GCCCTTTCAT GGCCAAGGGC GCCATATTGA ATTACATCAA GAGGAACTTC 
ACCAATGTTG GAGGATCTTT GCGAATAGGC AACGCTCTCC AGAAAGTCCA CAAGACCTAC 
TTCTCTGGGC CAGTCAGTGG AAGAGACAAG AGCCAGTTTC CCCAGATTCT GGTAGTTCTG 
GCTTCAGCCC ACTCTGAGGA TGATGTAAAG GGGCCAGCCA ACGCTCTGCA GAGAGATGGG 
GTGAAAATCA TCTCACTGGG GATGCAGAAT GCTTCGGAAG AAAACCTAAA AGCCATGGCC 
ACATCTAAGT TTCATTACAA CCTAAGAACA GTGAGAGATG TGGCCACATT TTCCACAAAT 
ATGACCAATA TCATTAAAGA AGTGGTAAAA TACAAGGGAG CAGATGATGC TGATGTGGAA 
GTTTGTCATG GCACTTCTGT GGCTGATGTC GTATTTCTCC TGGACAACTA TGTCAATGGC 
ACCAAGGAAA ATTTGGAATA TCTTAAAAGT TTCCTAGAAG AGAGCGTGTC CTCCTTTGAT 
GTGAAAGAGA GCTGTATGAG GATTGGCCTA GTGACCTACA GCAATGAGAC AAAGGTGATT 
CGTAACCTGG GCACTGGGAT CAACAAGTCA GAGATTCTGC AAGAGATAAA GAACCTATCT 
CCCAGAGCTG GGAGAGCTCG TGCTGGAGCC GCCATCACAA AGCTCAGGAA AGAGGTCTTC 
AATGTCCGCA GAGGCAGCCG AAAGATGCAG GGGGTCCCTC AAATAGCCAT TCTAGTCACT 
CACAGACCAT CAGAAGATAA TGTTTCTGTA GCTGCACTTG ACCTTCGGCG TGAAGGCGTG 
ACTGTGTTCA CTGTGGGCCT GGAAGGTTCT AATTATACCC AGTTAGAACA AATCTCATCC 
TACCCTCCAG AAAAGTATAT TTCCAAACTG AAAACATTTT CAGACTTGGC AGCCCATAAT 
CAGACATTTC GGAAGAAACT CAGGAATGAA ATACAGTACA AGGTATCCAT CCTGTCAGAA 
CGGACCGAAC ACCTGAAATC TGGTTGTGTG GAAACTGAAG ACGCGGACAT TTATTTACTC 
ATTGATGGCT CAGGAAGTAT CCACCCAACA GACTTCCAGG AAATGAAAAC ATTTTTGTCA 
GAAGTGATAG GAATGCTCAG CATAGCACCC CATAAAGTCA GAGTTGGAGC CGTTCAGTAT 
TCCGACAGAT GGCAACTGGA ATTTGAGATC AACCAATACT CAAACAAAAA TGACTTGGGC 
AAGGCTATTG ACAATATCCG GCAGATGGGA GGCAACACCA ACACAGGCGC AGCCCTGGAT 
TTTACTCTTG GGCTTCTCCA AGAAGCAAAG CGTCAACGAG GAAACAAGGT ACCATGTCAT 
CTGATTGTTT TGACAGATGG GTTGTCAGAT GATCCCGTTA AGGACCCTTC CAAGAAACTG 
CATGATGAGC ACATCGATGT GTTTGCCATT GGTGTCAAGG AGGCAAATCG AACTCAGTTG 
CTAGAAATTG CTGGGTCAGA AAAGAGAGTT TACTATGTAT ATAACTTTGA TTCTTTGAAA 
GACATAAAAA ATGAAGTTGT TCAAGGAATC TGTTCTGAAG AAGCTTGTAA AGATATGAGA 
TCCGATATCA TGTTTCTCGT GGATAGTTCT GGGAGTATAG GAGCTGAAAA TTTCGCTAAA 
ATGAAGACGT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGAGCAGA CCAAGTACAA 
ATTGGCGTCG TCCAGTTCAG CGATGTGAAC AGGGAAGAAT TTCAGCTCAA TAGATATAGG 
ACCCAACGTG AAATTGCTGA TGCAATAGAT AGAATGCCTC TCCTAGACCA GACAACCATG 
ACGGGAAGTG CTCTGACCTT TGTCTCTGAC TACTTCCATA CCTCCAAAGG GGCACGTCCC 
AATGTCAAAA AGTTTCTCAT CCTCATCACA GATGGAGAGG CTCAGGACCT GGTCAAGGAC 
CCAGCTCTGG CTCTTCGGCA AGATGGAATA ATTATCTACT CTGTGGGAGT GTATGGATCA 
AATCAAACCC AGCTAGTGGA AATCAGTGGG AGGCGTGATT TGGTTTTTTA TGTGGAGAAT 
TTTGATATTC TAAAGCGCAT AGAAGATCAA CTCATTTTTG GCATATGTAG CCCCCATGAA 
GAATGCAAAC AGATAGAAGT GTTAGATGTA GTATTCATCA TTGACAGCTC TGGCAGCATA 
GCCCCGGATG ACTATAACAT CATGAAGAAC TTCATGATTG AGCTGGTGAA AAAAGCCGAT 
GTTGGCAAGA ACCGAGTTCA GTTTGGAGCC CTGAAATACT CTAATGAGCC CGAGATAATT 
TTTTATCTTG ATGAACATGA CACAAAGTCC AAGGTGATTT CGGTTCTCCA GAGTGATTCT 
CCCCTAGGAG GAGACACGTA CACTGCCAAA GCCCTGGCCT TTTCGGAGCA CATGTTCACC 
GAGCCCCGGG GCAGCCGCAT TCACAGAAGG GTCCCCCAGG TCCTCATCGT GATCACAGAT 
GGGGTATCCC ACGACGCAGA CAAGCTCAAC GCCACGGCCA AAGCACTAAG GGACAAAGGA 
ATCCTCATCC TGGCAGTGGG CATCAAAGGA GCCAACACTA AGGAGCTGTT GGCAATAGCC 
GGATCGGAAG ACAAGTATTT CTTTGTGGAA ACCTTCGGGG GCCTGAAGGG CATATTCTCA 
AATGTGTCAG ACAGTATCTG TGCTCCTTCA AAAGTAGATT GTGAAATTGA AAGTGCAGAT 
CTTGTTTTCC TTGTTGATGG TTCTACTAGC ATACAACCAC ATGACTTCCA GAAAATGAAG 
GATTTTTTAG TATCTGTAGT AGATGATTTT GACATCAGAC CTAGCAAAGT GCATGTTGGA 
CTGGCTCAAT TCAGTGATAG CTACAAGTCT GAGTTTTATC TGGGGAAATT CACAACGGAA 
AAGGAAGTTT CTGCTCAAAT TGAAAGGATC TACCAGATCT TTGGAAATAC CCACATTGGA 
GCTGCGCTGA GGCAAGTGGA GGAATATTTC CGACCAGAGA TGGGCAGCAG AATAAATAGA 
GGCACCCAGC AGGTTTTATT GGTCCTCACT GATGGGCAAT CCCAAGATGA GTTGGCTAAG 
GCTGCTGAAG ACCTAAGAAG GAAAGGAATT GATATATACT CTTTGGGCAT TGGGGATGTA 
GATGAGCAGC AGCTCATTCA GATAACTGGA ACATCGGAGA AAAAACTAAC GGTCGATAAC 
TTTGACGAGC TGAAAAAGAT CAAAAAAAGG ATAGTCCGCA ACATCTGTAC ATCAGGCGGT 
GAAAGCAGTT GTTTTGTGGA TGTCGTCGTG GGCTTTGACA TCTCATCTCA ACAGAAAGGG 
CAGCCTTTAT TTCAAGGTCA GGCTTGGATG AAGACTTATC TTCAGGACGT GTTACGTGAC 
ATCAGTTCCC TAAATGGGGT GAGCTGTGAA GTGGGATCTC AGACTCAGGT CAGCGTGGCC 
TTTCAAGTGA CAAATGCCAT GGATAAATTT TCCCCCAAGT TTGAGATTTA CAGTGAAAGT 
ATCCTGAACT CCTTGAAGAA TGTATCCATT AAGGAGCCGT CTCGCCTCAA TGCACCTTTC 
TTGAATTCAC TTTGGGATGC CTTTCAGAAT AAATCTACTA CTCGAGGAAA GGTGCTGCTA 
TTATTTTCAG ATGGATTGGA TGATGATATT GAAGTACTTG AACAAAAATC TGATCAACTT 
CGAAAAGAAG GGCTCAACGC TTTGATAACC GTTGCTCTGG AAGAAGCTAC TGATGCAAAT 
GACCTAGCTG ATCTTCTGTA CATTGAATTT GGGAAAGGAT TTGATTATAG GACTCAGTTA 
ACAATCGGCA TGAGAGATTT GGGAAGCAGG CTCTCAAAAT ATCTGGCTAA TGTTGCTGAA 
AGGACATGTT GCTGTTTGCT TTGCAAGTGT TCTGGGGGAG ATGGTGCCGT TGGAGTACCA 
GGCACACCAG GGAAGAAGGG ACTTCTAGGT TTTAAAGGCA GTGAAGGCCA CTTGGGAGAA 
GAAGGTGTCC CTGGAGAAAG AGGCTCCCCT GGACCAGTGG GAGAGGAAGG CATTAAAGGA 
TGCCATGGGA GCAAAGGGCC CAAGGGAAAC AGGGGACTCA GTGGAGAGGA GGGAGAAATT 
GGGGAACTCG GGATTAATGG GATCAATGGA GAACAGGGTG AAGGCGGCCT TCCAGGAACA 
AAAGGAGAAA AAGGAGATGA AGGTTCCCAG GGCAACCCTG GGAAGAAAGG ACCCCCAGGT 
GACAGTGGCC AAAGGGGAGT GCGAGGAGAT CCTGGCAACC CTGGATCAGA CAATAACAGA 
AATGGACTCA CGGGCCCAAA AGGAGAAAGG GGAAGACAAG GAAGGCGTGG ACAGCCAGGG 
ACACCAGGAT CTCCGGGCTC CCGAGGAGAC ACACCAATAC ATGGCCGACG AGGACCAAGA 
GGCCCACAGG GGATAAATGG AATTTCTGGT CTGGTTGGTC ATCAAGGAGC ATTGGGATTA 
AAAGGACCTG AGGGCCCAAG AGGAGATCCT GGCACAAAAG GAGCAAAAGG AAATCTAGGA 
AATAAAGGCC CCTCGGGTCC TCCAGGACCA AATGGTGGTA AAGGGAGTCC TGGCAGCCTG 
GGGAGAAGAG GAAATAAGGG AGAGCCAGGG TCTCCAGGAG AGAAAGGAGC AGTTGGATCA 
TCTGGATATC GTGGAATGCC GGGAGAGGAT GGAAGCGCAG GGTATGGCAG AAGTGGAAGA 
AAAGGAGAAA AGGGACAAGA AGGATTCCCA GGAGATAGTG GACCAAAGGG GGAAGTTGGT 
GATCCTGGAA GGCCAGGAGA AGTTGGACCA AAAGGATATA AAGGAAGAAC GAGCCCTTCA 
GGACCACTGG GGGAACCAGG ATCTCCTGGA TCCCATGGAC CTTCAGGACA GAAGGGCCCA 
AAAGGAGCAA AGGGTTGGAC TTCATTTTCG ACCTGTGATG TCATTGAGTT TATACGGGAC 
CATAGCCCTG GGTGGCGCAG AAAGCCGGAA TGTCCCGTCC ATCCCACCGA GTTGGTGTTT 
GCTTTGGATC TGTCCCGAGA CGTGTCGGAG GAGGAGTTCG ATCGAATGAA GGAGATGACT 
ATTGCAATTG TGAACGACCT CCGAATCAGG GAAGGCAACT GCCCTGTGGG GGCCCGGGTG 
GCTGTGGTCT CCTACAACTC CAAGACCCGG CATCTCCTCC GGCTGTCTGA CTCCCGGGAC 
AAGAGCCAGC TGCTGCAGGA GATTAAAGCT CTCCGTTTTG AGCCCTCCTC AGGGGAGAGG 
GAGATTGGCA AGACCATGAG GTTCGTGGCC CGGAACGTCT TCAAGAGAAC CCTTCCCGGG 
GCCAACACAA GGAAGATCAC CACCGTTTTC AGCGCTGACA GGTCCGTGGA CGCGTCCTCC 
ATCGTCACGG CCACCATGGA ATTCAACGCT CTCGATGTCA TTCCCGTCGT GATTGCTTTT 
AACAACGTGC CTGCCGTCAA GCGCGCTTTC ACGATTGATG ACACCGGCAC CTTTCAGGTG 
CTGACCATCC CCCCGAGGGC CGACTACCGG CCGATGCTGA GGAGACTCCA GCAGTGCACG 
TTCTGCTACG ACAGATGCAA GCCAGAAGTT TCCTGTGAGG AGGCGAAACC CCCAGCGGTC 
CCATCCTATA TAGATGCGGC CTTCCTTTTC CAAGGTTCCC GAGATGTGGG AAGCGCCGAG 
TTTGAGGATG TGAAGGCTTT CCTGAAAGCA GTCTTGGACA ACTTTGACGT GGCCTTTGAA 
CCCGAGACCT CTGCCGTTGG GGATCGTGTT GCACTGCTGA GTCATGCTCC TCCGGGCTTT 
GTCCCAAATA GCCAGTCAAG CCCGGTCCGG ACGGAGTTCA ACTTCACCAC CTACAACAAT 
AAACGCCTGA TGAAGAGACA TGTGGAAGAA TCGGTTCAAC AGCTAAACGG GGAAGCTTTC 
TTGGGCCATG CCCTGCAGTG GACAGTGGAC AATATCTTTA CAGGCACCCC CAACCTGAGA 
AAAAACAGAG TCATTTTTGT GGTATCTGCT GGAGAAACAA GTTATTTGGA CAAGAAAGTC 
TTGAAGAGAG AATCTCTGCG GGCCAAATGT CAAGGTTACG CCATATTTGT GTTCTCTCTT 
GGCTTCTCAC ACAATGACGA GGAGCTCGAA GAATTAGCAA GCCTCCCCCT CGATCACCAC 
TTGGTCCAAC TTGGCCGAAT TCAGAAACCA GACCATGGCT ACGGGGTGAA GTTTGTGAAA 
TCCTTTATAA ACTCCGTCAG ACGTGCAATC AACAAGTATC CGCCAATAAA CTTTAAAGCA 
AAATGCAATA AAATCAATCT TCCGGAACCG GTGCAGCCTT CCAAACGGCA AAGCCACCTC 
CTTGCCCGTA TTGGACCACT CGAAGAGAGA TTTCTGTCCA AAAAGCTTCT CTCAAGAGTA 
ACACGGGGGA ATGCTGTTTG GAACATTCGT GGTTATACCA GGAATATGTC CCGTCCCCTT 
AAAACTGGAA ACAGAATGCC ACGCATTGTT CCCAAGACTC ATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012406800.1
CDS	1..6888
Translation	MVRLGDLSSLEARPEPLGARESLGPVPRLCVEAPAAGAGWGDYSPRASTAPAPLAALHAQFWLQGPEFADVVFLVDSSDHLASKFSFVRTFISKTINSLPIEANKFRIALAQYSDDLRQEFLLSPFMAKGAILNYIKRNFTNVGGSLRIGNALQKVHKTYFSGPVSGRDKSQFPQILVVLASAHSEDDVKGPANALQRDGVKIISLGMQNASEENLKAMATSKFHYNLRTVRDVATFSTNMTNIIKEVVKYKGADDADVEVCHGTSVADVVFLLDNYVNGTKENLEYLKSFLEESVSSFDVKESCMRIGLVTYSNETKVIRNLGTGINKSEILQEIKNLSPRAGRARAGAAITKLRKEVFNVRRGSRKMQGVPQIAILVTHRPSEDNVSVAALDLRREGVTVFTVGLEGSNYTQLEQISSYPPEKYISKLKTFSDLAAHNQTFRKKLRNEIQYKVSILSERTEHLKSGCVETEDADIYLLIDGSGSIHPTDFQEMKTFLSEVIGMLSIAPHKVRVGAVQYSDRWQLEFEINQYSNKNDLGKAIDNIRQMGGNTNTGAALDFTLGLLQEAKRQRGNKVPCHLIVLTDGLSDDPVKDPSKKLHDEHIDVFAIGVKEANRTQLLEIAGSEKRVYYVYNFDSLKDIKNEVVQGICSEEACKDMRSDIMFLVDSSGSIGAENFAKMKTFMKNLVSKSQIGADQVQIGVVQFSDVNREEFQLNRYRTQREIADAIDRMPLLDQTTMTGSALTFVSDYFHTSKGARPNVKKFLILITDGEAQDLVKDPALALRQDGIIIYSVGVYGSNQTQLVEISGRRDLVFYVENFDILKRIEDQLIFGICSPHEECKQIEVLDVVFIIDSSGSIAPDDYNIMKNFMIELVKKADVGKNRVQFGALKYSNEPEIIFYLDEHDTKSKVISVLQSDSPLGGDTYTAKALAFSEHMFTEPRGSRIHRRVPQVLIVITDGVSHDADKLNATAKALRDKGILILAVGIKGANTKELLAIAGSEDKYFFVETFGGLKGIFSNVSDSICAPSKVDCEIESADLVFLVDGSTSIQPHDFQKMKDFLVSVVDDFDIRPSKVHVGLAQFSDSYKSEFYLGKFTTEKEVSAQIERIYQIFGNTHIGAALRQVEEYFRPEMGSRINRGTQQVLLVLTDGQSQDELAKAAEDLRRKGIDIYSLGIGDVDEQQLIQITGTSEKKLTVDNFDELKKIKKRIVRNICTSGGESSCFVDVVVGFDISSQQKGQPLFQGQAWMKTYLQDVLRDISSLNGVSCEVGSQTQVSVAFQVTNAMDKFSPKFEIYSESILNSLKNVSIKEPSRLNAPFLNSLWDAFQNKSTTRGKVLLLFSDGLDDDIEVLEQKSDQLRKEGLNALITVALEEATDANDLADLLYIEFGKGFDYRTQLTIGMRDLGSRLSKYLANVAERTCCCLLCKCSGGDGAVGVPGTPGKKGLLGFKGSEGHLGEEGVPGERGSPGPVGEEGIKGCHGSKGPKGNRGLSGEEGEIGELGINGINGEQGEGGLPGTKGEKGDEGSQGNPGKKGPPGDSGQRGVRGDPGNPGSDNNRNGLTGPKGERGRQGRRGQPGTPGSPGSRGDTPIHGRRGPRGPQGINGISGLVGHQGALGLKGPEGPRGDPGTKGAKGNLGNKGPSGPPGPNGGKGSPGSLGRRGNKGEPGSPGEKGAVGSSGYRGMPGEDGSAGYGRSGRKGEKGQEGFPGDSGPKGEVGDPGRPGEVGPKGYKGRTSPSGPLGEPGSPGSHGPSGQKGPKGAKGWTSFSTCDVIEFIRDHSPGWRRKPECPVHPTELVFALDLSRDVSEEEFDRMKEMTIAIVNDLRIREGNCPVGARVAVVSYNSKTRHLLRLSDSRDKSQLLQEIKALRFEPSSGEREIGKTMRFVARNVFKRTLPGANTRKITTVFSADRSVDASSIVTATMEFNALDVIPVVIAFNNVPAVKRAFTIDDTGTFQVLTIPPRADYRPMLRRLQQCTFCYDRCKPEVSCEEAKPPAVPSYIDAAFLFQGSRDVGSAEFEDVKAFLKAVLDNFDVAFEPETSAVGDRVALLSHAPPGFVPNSQSSPVRTEFNFTTYNNKRLMKRHVEESVQQLNGEAFLGHALQWTVDNIFTGTPNLRKNRVIFVVSAGETSYLDKKVLKRESLRAKCQGYAIFVFSLGFSHNDEELEELASLPLDHHLVQLGRIQKPDHGYGVKFVKSFINSVRRAINKYPPINFKAKCNKINLPEPVQPSKRQSHLLARIGPLEERFLSKKLLSRVTRGNAVWNIRGYTRNMSRPLKTGNRMPRIVPKTHL

Target ORF information:

RefSeq Version	XM_012551346.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii collagen, type VI, alpha 6 (COL6A6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012551346.1

ORF Insert Sequence:

ATGGTGCGTC TCGGAGATCT CAGTTCCCTG GAAGCGCGGC CGGAGCCGCT GGGCGCCCGA 
GAGAGCTTGG GCCCGGTTCC CCGCCTCTGT GTGGAGGCTC CAGCTGCGGG GGCCGGGTGG 
GGTGATTACA GCCCTAGGGC TTCCACCGCC CCTGCACCTC TGGCTGCGCT CCACGCGCAG 
TTCTGGCTCC AGGGTCCTGA GTTTGCTGAT GTTGTGTTCC TGGTGGATAG CTCTGATCAC 
CTAGCGAGTA AGTTCTCTTT TGTGAGAACC TTCATCTCCA AGACGATCAA CAGTCTGCCC 
ATTGAAGCCA ACAAGTTCCG TATTGCCCTC GCCCAGTATA GCGATGATCT GCGCCAGGAG 
TTCCTGCTGA GCCCTTTCAT GGCCAAGGGC GCCATATTGA ATTACATCAA GAGGAACTTC 
ACCAATGTTG GAGGATCTTT GCGAATAGGC AACGCTCTCC AGAAAGTCCA CAAGACCTAC 
TTCTCTGGGC CAGTCAGTGG AAGAGACAAG AGCCAGTTTC CCCAGATTCT GGTAGTTCTG 
GCTTCAGCCC ACTCTGAGGA TGATGTAAAG GGGCCAGCCA ACGCTCTGCA GAGAGATGGG 
GTGAAAATCA TCTCACTGGG GATGCAGAAT GCTTCGGAAG AAAACCTAAA AGCCATGGCC 
ACATCTAAGT TTCATTACAA CCTAAGAACA GTGAGAGATG TGGCCACATT TTCCACAAAT 
ATGACCAATA TCATTAAAGA AGTGGTAAAA TACAAGGGAG CAGATGATGC TGATGTGGAA 
GTTTGTCATG GCACTTCTGT GGCTGATGTC GTATTTCTCC TGGACAACTA TGTCAATGGC 
ACCAAGGAAA ATTTGGAATA TCTTAAAAGT TTCCTAGAAG AGAGCGTGTC CTCCTTTGAT 
GTGAAAGAGA GCTGTATGAG GATTGGCCTA GTGACCTACA GCAATGAGAC AAAGGTGATT 
CGTAACCTGG GCACTGGGAT CAACAAGTCA GAGATTCTGC AAGAGATAAA GAACCTATCT 
CCCAGAGCTG GGAGAGCTCG TGCTGGAGCC GCCATCACAA AGCTCAGGAA AGAGGTCTTC 
AATGTCCGCA GAGGCAGCCG AAAGATGCAG GGGGTCCCTC AAATAGCCAT TCTAGTCACT 
CACAGACCAT CAGAAGATAA TGTTTCTGTA GCTGCACTTG ACCTTCGGCG TGAAGGCGTG 
ACTGTGTTCA CTGTGGGCCT GGAAGGTTCT AATTATACCC AGTTAGAACA AATCTCATCC 
TACCCTCCAG AAAAGTATAT TTCCAAACTG AAAACATTTT CAGACTTGGC AGCCCATAAT 
CAGACATTTC GGAAGAAACT CAGGAATGAA ATACAGTACA AGGTATCCAT CCTGTCAGAA 
CGGACCGAAC ACCTGAAATC TGGTTGTGTG GAAACTGAAG ACGCGGACAT TTATTTACTC 
ATTGATGGCT CAGGAAGTAT CCACCCAACA GACTTCCAGG AAATGAAAAC ATTTTTGTCA 
GAAGTGATAG GAATGCTCAG CATAGCACCC CATAAAGTCA GAGTTGGAGC CGTTCAGTAT 
TCCGACAGAT GGCAACTGGA ATTTGAGATC AACCAATACT CAAACAAAAA TGACTTGGGC 
AAGGCTATTG ACAATATCCG GCAGATGGGA GGCAACACCA ACACAGGCGC AGCCCTGGAT 
TTTACTCTTG GGCTTCTCCA AGAAGCAAAG CGTCAACGAG GAAACAAGGT ACCATGTCAT 
CTGATTGTTT TGACAGATGG GTTGTCAGAT GATCCCGTTA AGGACCCTTC CAAGAAACTG 
CATGATGAGC ACATCGATGT GTTTGCCATT GGTGTCAAGG AGGCAAATCG AACTCAGTTG 
CTAGAAATTG CTGGGTCAGA AAAGAGAGTT TACTATGTAT ATAACTTTGA TTCTTTGAAA 
GACATAAAAA ATGAAGTTGT TCAAGGAATC TGTTCTGAAG AAGCTTGTAA AGATATGAGA 
TCCGATATCA TGTTTCTCGT GGATAGTTCT GGGAGTATAG GAGCTGAAAA TTTCGCTAAA 
ATGAAGACGT TTATGAAAAA CCTGGTGAGC AAGTCTCAGA TTGGAGCAGA CCAAGTACAA 
ATTGGCGTCG TCCAGTTCAG CGATGTGAAC AGGGAAGAAT TTCAGCTCAA TAGATATAGG 
ACCCAACGTG AAATTGCTGA TGCAATAGAT AGAATGCCTC TCCTAGACCA GACAACCATG 
ACGGGAAGTG CTCTGACCTT TGTCTCTGAC TACTTCCATA CCTCCAAAGG GGCACGTCCC 
AATGTCAAAA AGTTTCTCAT CCTCATCACA GATGGAGAGG CTCAGGACCT GGTCAAGGAC 
CCAGCTCTGG CTCTTCGGCA AGATGGAATA ATTATCTACT CTGTGGGAGT GTATGGATCA 
AATCAAACCC AGCTAGTGGA AATCAGTGGG AGGCGTGATT TGGTTTTTTA TGTGGAGAAT 
TTTGATATTC TAAAGCGCAT AGAAGATCAA CTCATTTTTG GCATATGTAG CCCCCATGAA 
GAATGCAAAC AGATAGAAGT GTTAGATGTA GTATTCATCA TTGACAGCTC TGGCAGCATA 
GCCCCGGATG ACTATAACAT CATGAAGAAC TTCATGATTG AGCTGGTGAA AAAAGCCGAT 
GTTGGCAAGA ACCGAGTTCA GTTTGGAGCC CTGAAATACT CTAATGAGCC CGAGATAATT 
TTTTATCTTG ATGAACATGA CACAAAGTCC AAGGTGATTT CGGTTCTCCA GAGTGATTCT 
CCCCTAGGAG GAGACACGTA CACTGCCAAA GCCCTGGCCT TTTCGGAGCA CATGTTCACC 
GAGCCCCGGG GCAGCCGCAT TCACAGAAGG GTCCCCCAGG TCCTCATCGT GATCACAGAT 
GGGGTATCCC ACGACGCAGA CAAGCTCAAC GCCACGGCCA AAGCACTAAG GGACAAAGGA 
ATCCTCATCC TGGCAGTGGG CATCAAAGGA GCCAACACTA AGGAGCTGTT GGCAATAGCC 
GGATCGGAAG ACAAGTATTT CTTTGTGGAA ACCTTCGGGG GCCTGAAGGG CATATTCTCA 
AATGTGTCAG ACAGTATCTG TGCTCCTTCA AAAGTAGATT GTGAAATTGA AAGTGCAGAT 
CTTGTTTTCC TTGTTGATGG TTCTACTAGC ATACAACCAC ATGACTTCCA GAAAATGAAG 
GATTTTTTAG TATCTGTAGT AGATGATTTT GACATCAGAC CTAGCAAAGT GCATGTTGGA 
CTGGCTCAAT TCAGTGATAG CTACAAGTCT GAGTTTTATC TGGGGAAATT CACAACGGAA 
AAGGAAGTTT CTGCTCAAAT TGAAAGGATC TACCAGATCT TTGGAAATAC CCACATTGGA 
GCTGCGCTGA GGCAAGTGGA GGAATATTTC CGACCAGAGA TGGGCAGCAG AATAAATAGA 
GGCACCCAGC AGGTTTTATT GGTCCTCACT GATGGGCAAT CCCAAGATGA GTTGGCTAAG 
GCTGCTGAAG ACCTAAGAAG GAAAGGAATT GATATATACT CTTTGGGCAT TGGGGATGTA 
GATGAGCAGC AGCTCATTCA GATAACTGGA ACATCGGAGA AAAAACTAAC GGTCGATAAC 
TTTGACGAGC TGAAAAAGAT CAAAAAAAGG ATAGTCCGCA ACATCTGTAC ATCAGGCGGT 
GAAAGCAGTT GTTTTGTGGA TGTCGTCGTG GGCTTTGACA TCTCATCTCA ACAGAAAGGG 
CAGCCTTTAT TTCAAGGTCA GGCTTGGATG AAGACTTATC TTCAGGACGT GTTACGTGAC 
ATCAGTTCCC TAAATGGGGT GAGCTGTGAA GTGGGATCTC AGACTCAGGT CAGCGTGGCC 
TTTCAAGTGA CAAATGCCAT GGATAAATTT TCCCCCAAGT TTGAGATTTA CAGTGAAAGT 
ATCCTGAACT CCTTGAAGAA TGTATCCATT AAGGAGCCGT CTCGCCTCAA TGCACCTTTC 
TTGAATTCAC TTTGGGATGC CTTTCAGAAT AAATCTACTA CTCGAGGAAA GGTGCTGCTA 
TTATTTTCAG ATGGATTGGA TGATGATATT GAAGTACTTG AACAAAAATC TGATCAACTT 
CGAAAAGAAG GGCTCAACGC TTTGATAACC GTTGCTCTGG AAGAAGCTAC TGATGCAAAT 
GACCTAGCTG ATCTTCTGTA CATTGAATTT GGGAAAGGAT TTGATTATAG GACTCAGTTA 
ACAATCGGCA TGAGAGATTT GGGAAGCAGG CTCTCAAAAT ATCTGGCTAA TGTTGCTGAA 
AGGACATGTT GCTGTTTGCT TTGCAAGTGT TCTGGGGGAG ATGGTGCCGT TGGAGTACCA 
GGCACACCAG GGAAGAAGGG ACTTCTAGGT TTTAAAGGCA GTGAAGGCCA CTTGGGAGAA 
GAAGGTGTCC CTGGAGAAAG AGGCTCCCCT GGACCAGTGG GAGAGGAAGG CATTAAAGGA 
TGCCATGGGA GCAAAGGGCC CAAGGGAAAC AGGGGACTCA GTGGAGAGGA GGGAGAAATT 
GGGGAACTCG GGATTAATGG GATCAATGGA GAACAGGGTG AAGGCGGCCT TCCAGGAACA 
AAAGGAGAAA AAGGAGATGA AGGTTCCCAG GGCAACCCTG GGAAGAAAGG ACCCCCAGGT 
GACAGTGGCC AAAGGGGAGT GCGAGGAGAT CCTGGCAACC CTGGATCAGA CAATAACAGA 
AATGGACTCA CGGGCCCAAA AGGAGAAAGG GGAAGACAAG GAAGGCGTGG ACAGCCAGGG 
ACACCAGGAT CTCCGGGCTC CCGAGGAGAC ACACCAATAC ATGGCCGACG AGGACCAAGA 
GGCCCACAGG GGATAAATGG AATTTCTGGT CTGGTTGGTC ATCAAGGAGC ATTGGGATTA 
AAAGGACCTG AGGGCCCAAG AGGAGATCCT GGCACAAAAG GAGCAAAAGG AAATCTAGGA 
AATAAAGGCC CCTCGGGTCC TCCAGGACCA AATGGTGGTA AAGGGAGTCC TGGCAGCCTG 
GGGAGAAGAG GAAATAAGGG AGAGCCAGGG TCTCCAGGAG AGAAAGGAGC AGTTGGATCA 
TCTGGATATC GTGGAATGCC GGGAGAGGAT GGAAGCGCAG GGTATGGCAG AAGTGGAAGA 
AAAGGAGAAA AGGGACAAGA AGGATTCCCA GGAGATAGTG GACCAAAGGG GGAAGTTGGT 
GATCCTGGAA GGCCAGGAGA AGTTGGACCA AAAGGATATA AAGGAAGAAC GAGCCCTTCA 
GGACCACTGG GGGAACCAGG ATCTCCTGGA TCCCATGGAC CTTCAGGACA GAAGGGCCCA 
AAAGGAGCAA AGGGTTGGAC TTCATTTTCG ACCTGTGATG TCATTGAGTT TATACGGGAC 
CATAGCCCTG GGTGGCGCAG AAAGCCGGAA TGTCCCGTCC ATCCCACCGA GTTGGTGTTT 
GCTTTGGATC TGTCCCGAGA CGTGTCGGAG GAGGAGTTCG ATCGAATGAA GGAGATGACT 
ATTGCAATTG TGAACGACCT CCGAATCAGG GAAGGCAACT GCCCTGTGGG GGCCCGGGTG 
GCTGTGGTCT CCTACAACTC CAAGACCCGG CATCTCCTCC GGCTGTCTGA CTCCCGGGAC 
AAGAGCCAGC TGCTGCAGGA GATTAAAGCT CTCCGTTTTG AGCCCTCCTC AGGGGAGAGG 
GAGATTGGCA AGACCATGAG GTTCGTGGCC CGGAACGTCT TCAAGAGAAC CCTTCCCGGG 
GCCAACACAA GGAAGATCAC CACCGTTTTC AGCGCTGACA GGTCCGTGGA CGCGTCCTCC 
ATCGTCACGG CCACCATGGA ATTCAACGCT CTCGATGTCA TTCCCGTCGT GATTGCTTTT 
AACAACGTGC CTGCCGTCAA GCGCGCTTTC ACGATTGATG ACACCGGCAC CTTTCAGGTG 
CTGACCATCC CCCCGAGGGC CGACTACCGG CCGATGCTGA GGAGACTCCA GCAGTGCACG 
TTCTGCTACG ACAGATGCAA GCCAGAAGTT TCCTGTGAGG AGGCGAAACC CCCAGCGGTC 
CCATCCTATA TAGATGCGGC CTTCCTTTTC CAAGGTTCCC GAGATGTGGG AAGCGCCGAG 
TTTGAGGATG TGAAGGCTTT CCTGAAAGCA GTCTTGGACA ACTTTGACGT GGCCTTTGAA 
CCCGAGACCT CTGCCGTTGG GGATCGTGTT GCACTGCTGA GTCATGCTCC TCCGGGCTTT 
GTCCCAAATA GCCAGTCAAG CCCGGTCCGG ACGGAGTTCA ACTTCACCAC CTACAACAAT 
AAACGCCTGA TGAAGAGACA TGTGGAAGAA TCGGTTCAAC AGCTAAACGG GGAAGCTTTC 
TTGGGCCATG CCCTGCAGTG GACAGTGGAC AATATCTTTA CAGGCACCCC CAACCTGAGA 
AAAAACAGAG TCATTTTTGT GGTATCTGCT GGAGAAACAA GTTATTTGGA CAAGAAAGTC 
TTGAAGAGAG AATCTCTGCG GGCCAAATGT CAAGGTTACG CCATATTTGT GTTCTCTCTT 
GGCTTCTCAC ACAATGACGA GGAGCTCGAA GAATTAGCAA GCCTCCCCCT CGATCACCAC 
TTGGTCCAAC TTGGCCGAAT TCAGAAACCA GACCATGGCT ACGGGGTGAA GTTTGTGAAA 
TCCTTTATAA ACTCCGTCAG ACGTGCAATC AACAAGTATC CGCCAATAAA CTTTAAAGCA 
AAATGCAATA AAATCAATCT TCCGGAACCG GTGCAGCCTT CCAAACGGCA AAGCCACCTC 
CTTGCCCGTA TTGGACCACT CGAAGAGAGA TTTCTGTCCA AAAAGCTTCT CTCAAGAGTA 
ACACGGGGGA ATGCTGTTTG GAACATTCGT GGTTATACCA GGAATATGTC CCGTCCCCTT 
AAAACTGGAA ACAGAATGCC ACGCATTGTT CCCAAGACTC ATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_023505163.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023360931.1 Sarcophilus harrisii collagen type VI alpha 6 chain (COL6A6), mRNA.

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CloneID	OSa156540
Clone ID Related Accession (Same CDS sequence)	XM_023505163.1 , XM_023505163.2
Accession Version	XM_023505163.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	collagen alpha-6(VI) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003843704.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTGT TTATCTTCCT CATCATAACT TGTGTCCAAG CACAGTCTGT GACCCAGGAT 
GTTGGTCCTG AGTTTGCTGA TGTTGTGTTC CTGGTGGATA GCTCTGATCA CCTAGCGAGT 
AAGTTCTCTT TTGTGAGAAC CTTCATCTCC AAGACGATCA ACAGTCTGCC CATTGAAGCC 
AACAAGTTCC GTATTGCCCT CGCCCAGTAT AGCGATGATC TGCGCCAGGA GTTCCTGCTG 
AGCCCTTTCA TGGCCAAGGG CGCCATATTG AATTACATCA AGAGGAACTT CACCAATGTT 
GGAGGATCTT TGCGAATAGG CAACGCTCTC CAGAAAGTCC ACAAGACCTA CTTCTCTGGG 
CCAGTCAGTG GAAGAGACAA GAGCCAGTTT CCCCAGATTC TGGTAGTTCT GGCTTCAGCC 
CACTCTGAGG ATGATGTAAA GGGGCCAGCC AACGCTCTGC AGAGAGATGG GGTGAAAATC 
ATCTCACTGG GGATGCAGAA TGCTTCGGAA GAAAACCTAA AAGCCATGGC CACATCTAAG 
TTTCATTACA ACCTAAGAAC AGTGAGAGAT GTGGCCACAT TTTCCACAAA TATGACCAAT 
ATCATTAAAG AAGTGGTAAA ATACAAGGGA GCAGATGATG CTGATGTGGA AGTTTGTCAT 
GGCACTTCTG TGGCTGATGT CGTATTTCTC CTGGACAACT ATGTCAATGG CACCAAGGAA 
AATTTGGAAT ATCTTAAAAG TTTCCTAGAA GAGAGCGTGT CCTCCTTTGA TGTGAAAGAG 
AGCTGTATGA GGATTGGCCT AGTGACCTAC AGCAATGAGA CAAAGGTGAT TCGTAACCTG 
GGCACTGGGA TCAACAAGTC AGAGATTCTG CAAGAGATAA AGAACCTATC TCCCAGAGCT 
GGGAGAGCTC GTGCTGGAGC CGCCATCACA AAGCTCAGGA AAGAGGTCTT CAATGTCCGC 
AGAGGCAGCC GAAAGATGCA GGGGGTCCCT CAAATAGCCA TTCTAGTCAC TCACAGACCA 
TCAGAAGATA ATGTTTCTGT AGCTGCACTT GACCTTCGGC GTGAAGGCGT GACTGTGTTC 
ACTGTGGGCC TGGAAGGTTC TAATTATACC CAGTTAGAAC AAATCTCATC CTACCCTCCA 
GAAAAGTATA TTTCCAAACT GAAAACATTT TCAGACTTGG CAGCCCATAA TCAGACATTT 
CGGAAGAAAC TCAGGAATGA AATACAGTAC AAGGTATCCA TCCTGTCAGA ACGGACCGAA 
CACCTGAAAT CTGGTTGTGT GGAAACTGAA GACGCGGACA TTTATTTACT CATTGATGGC 
TCAGGAAGTA TCCACCCAAC AGACTTCCAG GAAATGAAAA CATTTTTGTC AGAAGTGATA 
GGAATGCTCA GCATAGCACC CCATAAAGTC AGAGTTGGAG CCGTTCAGTA TTCCGACAGA 
TGGCAACTGG AATTTGAGAT CAACCAATAC TCAAACAAAA ATGACTTGGG CAAGGCTATT 
GACAATATCC GGCAGATGGG AGGCAACACC AACACAGGCG CAGCCCTGGA TTTTACTCTT 
GGGCTTCTCC AAGAAGCAAA GCGTCAACGA GGAAACAAGG TACCATGTCA TCTGATTGTT 
TTGACAGATG GGTTGTCAGA TGATCCCGTT AAGGACCCTT CCAAGAAACT GCATGATGAG 
CACATCGATG TGTTTGCCAT TGGTGTCAAG GAGGCAAATC GAACTCAGTT GCTAGAAATT 
GCTGGGTCAG AAAAGAGAGT TTACTATGTA TATAACTTTG ATTCTTTGAA AGACATAAAA 
AATGAAGTTG TTCAAGGAAT CTGTTCTGAA GAAGCTTGTA AAGATATGAG ATCCGATATC 
ATGTTTCTCG TGGATAGTTC TGGGAGTATA GGAGCTGAAA ATTTCGCTAA AATGAAGACG 
TTTATGAAAA ACCTGGTGAG CAAGTCTCAG ATTGGAGCAG ACCAAGTACA AATTGGCGTC 
GTCCAGTTCA GCGATGTGAA CAGGGAAGAA TTTCAGCTCA ATAGATATAG GACCCAACGT 
GAAATTGCTG ATGCAATAGA TAGAATGCCT CTCCTAGACC AGACAACCAT GACGGGAAGT 
GCTCTGACCT TTGTCTCTGA CTACTTCCAT ACCTCCAAAG GGGCACGTCC CAATGTCAAA 
AAGTTTCTCA TCCTCATCAC AGATGGAGAG GCTCAGGACC TGGTCAAGGA CCCAGCTCTG 
GCTCTTCGGC AAGATGGAAT AATTATCTAC TCTGTGGGAG TGTATGGATC AAATCAAACC 
CAGCTAGTGG AAATCAGTGG GAGGCGTGAT TTGGTTTTTT ATGTGGAGAA TTTTGATATT 
CTAAAGCGCA TAGAAGATCA ACTCATTTTT GGCATATGTA GCCCCCATGA AGAATGCAAA 
CAGATAGAAG TGTTAGATGT AGTATTCATC ATTGACAGCT CTGGCAGCAT AGCCCCGGAT 
GACTATAACA TCATGAAGAA CTTCATGATT GAGCTGGTGA AAAAAGCCGA TGTTGGCAAG 
AACCGAGTTC AGTTTGGAGC CCTGAAATAC TCTAATGAGC CCGAGATAAT TTTTTATCTT 
GATGAACATG ACACAAAGTC CAAGGTGATT TCGGTTCTCC AGAGTGATTC TCCCCTAGGA 
GGAGACACGT ACACTGCCAA AGCCCTGGCC TTTTCGGAGC ACATGTTCAC CGAGCCCCGG 
GGCAGCCGCA TTCACAGAAG GGTCCCCCAG GTCCTCATCG TGATCACAGA TGGGGTATCC 
CACGACGCAG ACAAGCTCAA CGCCACGGCC AAAGCACTAA GGGACAAAGG AATCCTCATC 
CTGGCAGTGG GCATCAAAGG AGCCAACACT AAGGAGCTGT TGGCAATAGC CGGATCGGAA 
GACAAGTATT TCTTTGTGGA AACCTTCGGG GGCCTGAAGG GCATATTCTC AAATGTGTCA 
GACAGTATCT GTGCTCCTTC AAAAGTAGAT TGTGAAATTG AAAGTGCAGA TCTTGTTTTC 
CTTGTTGATG GTTCTACTAG CATACAACCA CATGACTTCC AGAAAATGAA GGATTTTTTA 
GTATCTGTAG TAGATGATTT TGACATCAGA CCTAGCAAAG TGCATGTTGG ACTGGCTCAA 
TTCAGTGATA GCTACAAGTC TGAGTTTTAT CTGGGGAAAT TCACAACGGA AAAGGAAGTT 
TCTGCTCAAA TTGAAAGGAT CTACCAGATC TTTGGAAATA CCCACATTGG AGCTGCGCTG 
AGGCAAGTGG AGGAATATTT CCGACCAGAG ATGGGCAGCA GAATAAATAG AGGCACCCAG 
CAGGTTTTAT TGGTCCTCAC TGATGGGCAA TCCCAAGATG AGTTGGCTAA GGCTGCTGAA 
GACCTAAGAA GGAAAGGAAT TGATATATAC TCTTTGGGCA TTGGGGATGT AGATGAGCAG 
CAGCTCATTC AGATAACTGG AACATCGGAG AAAAAACTAA CGGTCGATAA CTTTGACGAG 
CTGAAAAAGA TCAAAAAAAG GATAGTCCGC AACATCTGTA CATCAGGCGG TGAAAGCAGT 
TGTTTTGTGG ATGTCGTCGT GGGCTTTGAC ATCTCATCTC AACAGAAAGG GCAGCCTTTA 
TTTCAAGGTC AGGCTTGGAT GAAGACTTAT CTTCAGGACG TGTTACGTGA CATCAGTTCC 
CTAAATGGGG TGAGCTGTGA AGTGGGATCT CAGACTCAGG TCAGCGTGGC CTTTCAAGTG 
ACAAATGCCA TGGATAAATT TTCCCCCAAG TTTGAGATTT ACAGTGAAAG TATCCTGAAC 
TCCTTGAAGA ATGTATCCAT TAAGGAGCCG TCTCGCCTCA ATGCACCTTT CTTGAATTCA 
CTTTGGGATG CCTTTCAGAA TAAATCTACT ACTCGAGGAA AGGTGCTGCT ATTATTTTCA 
GATGGATTGG ATGATGATAT TGAAGTACTT GAACAAAAAT CTGATCAACT TCGAAAAGAA 
GGGCTCAACG CTTTGATAAC CGTTGCTCTG GAAGAAGCTA CTGATGCAAA TGACCTAGCT 
GATCTTCTGT ACATTGAATT TGGGAAAGGA TTTGATTATA GGACTCAGTT AACAATCGGC 
ATGAGAGATT TGGGAAGCAG GCTCTCAAAA TATCTGGCTA ATGTTGCTGA AAGGACATGT 
TGCTGTTTGC TTTGCAAGTG TTCTGGGGGA GATGGTGCCG TTGGAGTACC AGGCACACCA 
GGGAAGAAGG GACTTCTAGG TTTTAAAGGC AGTGAAGGCC ACTTGGGAGA AGAAGGTGTC 
CCTGGAGAAA GAGGCTCCCC TGGACCAGTG GGAGAGGAAG GCATTAAAGG ATGCCATGGG 
AGCAAAGGGC CCAAGGGAAA CAGGGGACTC AGTGGAGAGG AGGGAGAAAT TGGGGAACTC 
GGGATTAATG GGATCAATGG AGAACAGGGT GAAGGCGGCC TTCCAGGAAC AAAAGGAGAA 
AAAGGAGATG AAGGTTCCCA GGGCAACCCT GGGAAGAAAG GACCCCCAGG TGACAGTGGC 
CAAAGGGGAG TGCGAGGAGA TCCTGGCAAC CCTGGATCAG ACAATAACAG AAATGGACTC 
ACGGGCCCAA AAGGAGAAAG GGGAAGACAA GGAAGGCGTG GACAGCCAGG GACACCAGGA 
TCTCCGGGCT CCCGAGGAGA CACACCAATA CATGGCCGAC GAGGACCAAG AGGCCCACAG 
GGGATAAATG GAATTTCTGG TCTGGTTGGT CATCAAGGAG CATTGGGATT AAAAGGACCT 
GAGGGCCCAA GAGGAGATCC TGGCACAAAA GGAGCAAAAG GAAATCTAGG AAATAAAGGC 
CCCTCGGGTC CTCCAGGACC AAATGGTGGT AAAGGGAGTC CTGGCAGCCT GGGGAGAAGA 
GGAAATAAGG GAGAGCCAGG GTCTCCAGGA GAGAAAGGAG CAGTTGGATC ATCTGGATAT 
CGTGGAATGC CGGGAGAGGA TGGAAGCGCA GGGTATGGCA GAAGTGGAAG AAAAGGAGAA 
AAGGGACAAG AAGGATTCCC AGGAGATAGT GGACCAAAGG GGGAAGTTGG TGATCCTGGA 
AGGCCAGGAG AAGTTGGACC AAAAGGATAT AAAGGAAGAA CGAGCCCTTC AGGACCACTG 
GGGGAACCAG GATCTCCTGG ATCCCATGGA CCTTCAGGAC AGAAGGGCCC AAAAGGAGCA 
AAGGGTTGGA CTTCATTTTC GACCTGTGAT GTCATTGAGT TTATACGGGA CCATAGCCCT 
TGCTGGAAGG GAAAGCCGGA ATGTCCCGTC CATCCCACCG AGTTGGTGTT TGCTTTGGAT 
CTGTCCCGAG ACGTGTCGGA GGAGGAGTTC GATCGAATGA AGGAGATGAC TATTGCAATT 
GTGAACGACC TCCGAATCAG GGAAGGCAAC TGCCCTGTGG GGGCCCGGGT GGCTGTGGTC 
TCCTACAACT CCAAGACCCG GCATCTCCTC CGGCTGTCTG ACTCCCGGGA CAAGAGCCAG 
CTGCTGCAGG AGATTAAAGC TCTCCGTTTT GAGCCCTCCT CAGGGGAGAG GGAGATTGGC 
AAGACCATGA GGTTCGTGGC CCGGAACGTC TTCAAGAGAA CCCTTCCCGG GGCCAACACA 
AGGAAGATCA CCACCGTTTT CAGCGCTGAC AGGTCCGTGG ACGCGTCCTC CATCGTCACG 
GCCACCATGG AATTCAACGC TCTCGATGTC ATTCCCGTCG TGATTGCTTT TAACAACGTG 
CCTGCCGTCA AGCGCGCTTT CACGATTGAT GACACCGGCA CCTTTCAGGT GCTGACCATC 
CCCCCGAGGG CCGACTACCG GCCGATGCTG AGGAGACTCC AGCAGTGCAC GTTCTGCTAC 
GACAGATGCA AGCCAGAAGT TTCCTGTGAG GAGGCGAAAC CCCCAGCGGT CCCATCCTAT 
ATAGATGCGG CCTTCCTTTT CCAAGGTTCC CGAGATGTGG GAAGCGCCGA GTTTGAGGAT 
GTGAAGGCTT TCCTGAAAGC AGTCTTGGAC AACTTTGACG TGGCCTTTGA ACCCGAGACC 
TCTGCCGTTG GGGATCGTGT TGCACTGCTG AGTCATGCTC CTCCGGGCTT TGTCCCAAAT 
AGCCAGTCAA GCCCGGTCCG GACGGAGTTC AACTTCACCA CCTACAACAA TAAACGCCTG 
ATGAAGAGAC ATGTGGAAGA ATCGGTTCAA CAGCTAAACG GGGAAGCTTT CTTGGGCCAT 
GCCCTGCAGT GGACAGTGGA CAATATCTTT ACAGGCACCC CCAACCTGAG AAAAAACAGA 
GTCATTTTTG TGGTATCTGC TGGAGAAACA AGTTATTTGG ACAAGAAAGT CTTGAAGAGA 
GAATCTCTGC GGGCCAAATG TCAAGGTTAC GCCATATTTG TGTTCTCTCT TGGCTTCTCA 
CACAATGACG AGGAGCTCGA AGAATTAGCA AGCCTCCCCC TCGATCACCA CTTGGTCCAA 
CTTGGCCGAA TTCAGAAACC AGACCATGGC TACGGGGTGA AGTTTGTGAA ATCCTTTATA 
AACTCCGTCA GACGTGCAAT CAACAAGTAT CCGCCAATAA ACTTTAAAGC AAAATGCAAT 
AAAATCAATC TTCCGGAACC GGTGCAGCCT TCCAAACGGC AAAGCCACCT CCTTGCCCGT 
ATTGGACCAC TCGAAGAGAG ATTTCTGTCC AAAAAGCTTC TCTCAAGAGT AACACGGGGG 
AATGCTGTTT GGAACATTCG TGGTTATACC AGGAATATGT CCCGTCCCCT TAAAACTGGA 
AACAGAATGC CACGCATTGT TCCCAAGACT CATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023360931.1
CDS	231..6989
Translation	MFLFIFLIITCVQAQSVTQDVGPEFADVVFLVDSSDHLASKFSFVRTFISKTINSLPIEANKFRIALAQYSDDLRQEFLLSPFMAKGAILNYIKRNFTNVGGSLRIGNALQKVHKTYFSGPVSGRDKSQFPQILVVLASAHSEDDVKGPANALQRDGVKIISLGMQNASEENLKAMATSKFHYNLRTVRDVATFSTNMTNIIKEVVKYKGADDADVEVCHGTSVADVVFLLDNYVNGTKENLEYLKSFLEESVSSFDVKESCMRIGLVTYSNETKVIRNLGTGINKSEILQEIKNLSPRAGRARAGAAITKLRKEVFNVRRGSRKMQGVPQIAILVTHRPSEDNVSVAALDLRREGVTVFTVGLEGSNYTQLEQISSYPPEKYISKLKTFSDLAAHNQTFRKKLRNEIQYKVSILSERTEHLKSGCVETEDADIYLLIDGSGSIHPTDFQEMKTFLSEVIGMLSIAPHKVRVGAVQYSDRWQLEFEINQYSNKNDLGKAIDNIRQMGGNTNTGAALDFTLGLLQEAKRQRGNKVPCHLIVLTDGLSDDPVKDPSKKLHDEHIDVFAIGVKEANRTQLLEIAGSEKRVYYVYNFDSLKDIKNEVVQGICSEEACKDMRSDIMFLVDSSGSIGAENFAKMKTFMKNLVSKSQIGADQVQIGVVQFSDVNREEFQLNRYRTQREIADAIDRMPLLDQTTMTGSALTFVSDYFHTSKGARPNVKKFLILITDGEAQDLVKDPALALRQDGIIIYSVGVYGSNQTQLVEISGRRDLVFYVENFDILKRIEDQLIFGICSPHEECKQIEVLDVVFIIDSSGSIAPDDYNIMKNFMIELVKKADVGKNRVQFGALKYSNEPEIIFYLDEHDTKSKVISVLQSDSPLGGDTYTAKALAFSEHMFTEPRGSRIHRRVPQVLIVITDGVSHDADKLNATAKALRDKGILILAVGIKGANTKELLAIAGSEDKYFFVETFGGLKGIFSNVSDSICAPSKVDCEIESADLVFLVDGSTSIQPHDFQKMKDFLVSVVDDFDIRPSKVHVGLAQFSDSYKSEFYLGKFTTEKEVSAQIERIYQIFGNTHIGAALRQVEEYFRPEMGSRINRGTQQVLLVLTDGQSQDELAKAAEDLRRKGIDIYSLGIGDVDEQQLIQITGTSEKKLTVDNFDELKKIKKRIVRNICTSGGESSCFVDVVVGFDISSQQKGQPLFQGQAWMKTYLQDVLRDISSLNGVSCEVGSQTQVSVAFQVTNAMDKFSPKFEIYSESILNSLKNVSIKEPSRLNAPFLNSLWDAFQNKSTTRGKVLLLFSDGLDDDIEVLEQKSDQLRKEGLNALITVALEEATDANDLADLLYIEFGKGFDYRTQLTIGMRDLGSRLSKYLANVAERTCCCLLCKCSGGDGAVGVPGTPGKKGLLGFKGSEGHLGEEGVPGERGSPGPVGEEGIKGCHGSKGPKGNRGLSGEEGEIGELGINGINGEQGEGGLPGTKGEKGDEGSQGNPGKKGPPGDSGQRGVRGDPGNPGSDNNRNGLTGPKGERGRQGRRGQPGTPGSPGSRGDTPIHGRRGPRGPQGINGISGLVGHQGALGLKGPEGPRGDPGTKGAKGNLGNKGPSGPPGPNGGKGSPGSLGRRGNKGEPGSPGEKGAVGSSGYRGMPGEDGSAGYGRSGRKGEKGQEGFPGDSGPKGEVGDPGRPGEVGPKGYKGRTSPSGPLGEPGSPGSHGPSGQKGPKGAKGWTSFSTCDVIEFIRDHSPCWKGKPECPVHPTELVFALDLSRDVSEEEFDRMKEMTIAIVNDLRIREGNCPVGARVAVVSYNSKTRHLLRLSDSRDKSQLLQEIKALRFEPSSGEREIGKTMRFVARNVFKRTLPGANTRKITTVFSADRSVDASSIVTATMEFNALDVIPVVIAFNNVPAVKRAFTIDDTGTFQVLTIPPRADYRPMLRRLQQCTFCYDRCKPEVSCEEAKPPAVPSYIDAAFLFQGSRDVGSAEFEDVKAFLKAVLDNFDVAFEPETSAVGDRVALLSHAPPGFVPNSQSSPVRTEFNFTTYNNKRLMKRHVEESVQQLNGEAFLGHALQWTVDNIFTGTPNLRKNRVIFVVSAGETSYLDKKVLKRESLRAKCQGYAIFVFSLGFSHNDEELEELASLPLDHHLVQLGRIQKPDHGYGVKFVKSFINSVRRAINKYPPINFKAKCNKINLPEPVQPSKRQSHLLARIGPLEERFLSKKLLSRVTRGNAVWNIRGYTRNMSRPLKTGNRMPRIVPKTHL

Target ORF information:

RefSeq Version	XM_023505163.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii collagen type VI alpha 6 chain (COL6A6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023505163.1

ORF Insert Sequence:

ATGTTCCTGT TTATCTTCCT CATCATAACT TGTGTCCAAG CACAGTCTGT GACCCAGGAT 
GTTGGTCCTG AGTTTGCTGA TGTTGTGTTC CTGGTGGATA GCTCTGATCA CCTAGCGAGT 
AAGTTCTCTT TTGTGAGAAC CTTCATCTCC AAGACGATCA ACAGTCTGCC CATTGAAGCC 
AACAAGTTCC GTATTGCCCT CGCCCAGTAT AGCGATGATC TGCGCCAGGA GTTCCTGCTG 
AGCCCTTTCA TGGCCAAGGG CGCCATATTG AATTACATCA AGAGGAACTT CACCAATGTT 
GGAGGATCTT TGCGAATAGG CAACGCTCTC CAGAAAGTCC ACAAGACCTA CTTCTCTGGG 
CCAGTCAGTG GAAGAGACAA GAGCCAGTTT CCCCAGATTC TGGTAGTTCT GGCTTCAGCC 
CACTCTGAGG ATGATGTAAA GGGGCCAGCC AACGCTCTGC AGAGAGATGG GGTGAAAATC 
ATCTCACTGG GGATGCAGAA TGCTTCGGAA GAAAACCTAA AAGCCATGGC CACATCTAAG 
TTTCATTACA ACCTAAGAAC AGTGAGAGAT GTGGCCACAT TTTCCACAAA TATGACCAAT 
ATCATTAAAG AAGTGGTAAA ATACAAGGGA GCAGATGATG CTGATGTGGA AGTTTGTCAT 
GGCACTTCTG TGGCTGATGT CGTATTTCTC CTGGACAACT ATGTCAATGG CACCAAGGAA 
AATTTGGAAT ATCTTAAAAG TTTCCTAGAA GAGAGCGTGT CCTCCTTTGA TGTGAAAGAG 
AGCTGTATGA GGATTGGCCT AGTGACCTAC AGCAATGAGA CAAAGGTGAT TCGTAACCTG 
GGCACTGGGA TCAACAAGTC AGAGATTCTG CAAGAGATAA AGAACCTATC TCCCAGAGCT 
GGGAGAGCTC GTGCTGGAGC CGCCATCACA AAGCTCAGGA AAGAGGTCTT CAATGTCCGC 
AGAGGCAGCC GAAAGATGCA GGGGGTCCCT CAAATAGCCA TTCTAGTCAC TCACAGACCA 
TCAGAAGATA ATGTTTCTGT AGCTGCACTT GACCTTCGGC GTGAAGGCGT GACTGTGTTC 
ACTGTGGGCC TGGAAGGTTC TAATTATACC CAGTTAGAAC AAATCTCATC CTACCCTCCA 
GAAAAGTATA TTTCCAAACT GAAAACATTT TCAGACTTGG CAGCCCATAA TCAGACATTT 
CGGAAGAAAC TCAGGAATGA AATACAGTAC AAGGTATCCA TCCTGTCAGA ACGGACCGAA 
CACCTGAAAT CTGGTTGTGT GGAAACTGAA GACGCGGACA TTTATTTACT CATTGATGGC 
TCAGGAAGTA TCCACCCAAC AGACTTCCAG GAAATGAAAA CATTTTTGTC AGAAGTGATA 
GGAATGCTCA GCATAGCACC CCATAAAGTC AGAGTTGGAG CCGTTCAGTA TTCCGACAGA 
TGGCAACTGG AATTTGAGAT CAACCAATAC TCAAACAAAA ATGACTTGGG CAAGGCTATT 
GACAATATCC GGCAGATGGG AGGCAACACC AACACAGGCG CAGCCCTGGA TTTTACTCTT 
GGGCTTCTCC AAGAAGCAAA GCGTCAACGA GGAAACAAGG TACCATGTCA TCTGATTGTT 
TTGACAGATG GGTTGTCAGA TGATCCCGTT AAGGACCCTT CCAAGAAACT GCATGATGAG 
CACATCGATG TGTTTGCCAT TGGTGTCAAG GAGGCAAATC GAACTCAGTT GCTAGAAATT 
GCTGGGTCAG AAAAGAGAGT TTACTATGTA TATAACTTTG ATTCTTTGAA AGACATAAAA 
AATGAAGTTG TTCAAGGAAT CTGTTCTGAA GAAGCTTGTA AAGATATGAG ATCCGATATC 
ATGTTTCTCG TGGATAGTTC TGGGAGTATA GGAGCTGAAA ATTTCGCTAA AATGAAGACG 
TTTATGAAAA ACCTGGTGAG CAAGTCTCAG ATTGGAGCAG ACCAAGTACA AATTGGCGTC 
GTCCAGTTCA GCGATGTGAA CAGGGAAGAA TTTCAGCTCA ATAGATATAG GACCCAACGT 
GAAATTGCTG ATGCAATAGA TAGAATGCCT CTCCTAGACC AGACAACCAT GACGGGAAGT 
GCTCTGACCT TTGTCTCTGA CTACTTCCAT ACCTCCAAAG GGGCACGTCC CAATGTCAAA 
AAGTTTCTCA TCCTCATCAC AGATGGAGAG GCTCAGGACC TGGTCAAGGA CCCAGCTCTG 
GCTCTTCGGC AAGATGGAAT AATTATCTAC TCTGTGGGAG TGTATGGATC AAATCAAACC 
CAGCTAGTGG AAATCAGTGG GAGGCGTGAT TTGGTTTTTT ATGTGGAGAA TTTTGATATT 
CTAAAGCGCA TAGAAGATCA ACTCATTTTT GGCATATGTA GCCCCCATGA AGAATGCAAA 
CAGATAGAAG TGTTAGATGT AGTATTCATC ATTGACAGCT CTGGCAGCAT AGCCCCGGAT 
GACTATAACA TCATGAAGAA CTTCATGATT GAGCTGGTGA AAAAAGCCGA TGTTGGCAAG 
AACCGAGTTC AGTTTGGAGC CCTGAAATAC TCTAATGAGC CCGAGATAAT TTTTTATCTT 
GATGAACATG ACACAAAGTC CAAGGTGATT TCGGTTCTCC AGAGTGATTC TCCCCTAGGA 
GGAGACACGT ACACTGCCAA AGCCCTGGCC TTTTCGGAGC ACATGTTCAC CGAGCCCCGG 
GGCAGCCGCA TTCACAGAAG GGTCCCCCAG GTCCTCATCG TGATCACAGA TGGGGTATCC 
CACGACGCAG ACAAGCTCAA CGCCACGGCC AAAGCACTAA GGGACAAAGG AATCCTCATC 
CTGGCAGTGG GCATCAAAGG AGCCAACACT AAGGAGCTGT TGGCAATAGC CGGATCGGAA 
GACAAGTATT TCTTTGTGGA AACCTTCGGG GGCCTGAAGG GCATATTCTC AAATGTGTCA 
GACAGTATCT GTGCTCCTTC AAAAGTAGAT TGTGAAATTG AAAGTGCAGA TCTTGTTTTC 
CTTGTTGATG GTTCTACTAG CATACAACCA CATGACTTCC AGAAAATGAA GGATTTTTTA 
GTATCTGTAG TAGATGATTT TGACATCAGA CCTAGCAAAG TGCATGTTGG ACTGGCTCAA 
TTCAGTGATA GCTACAAGTC TGAGTTTTAT CTGGGGAAAT TCACAACGGA AAAGGAAGTT 
TCTGCTCAAA TTGAAAGGAT CTACCAGATC TTTGGAAATA CCCACATTGG AGCTGCGCTG 
AGGCAAGTGG AGGAATATTT CCGACCAGAG ATGGGCAGCA GAATAAATAG AGGCACCCAG 
CAGGTTTTAT TGGTCCTCAC TGATGGGCAA TCCCAAGATG AGTTGGCTAA GGCTGCTGAA 
GACCTAAGAA GGAAAGGAAT TGATATATAC TCTTTGGGCA TTGGGGATGT AGATGAGCAG 
CAGCTCATTC AGATAACTGG AACATCGGAG AAAAAACTAA CGGTCGATAA CTTTGACGAG 
CTGAAAAAGA TCAAAAAAAG GATAGTCCGC AACATCTGTA CATCAGGCGG TGAAAGCAGT 
TGTTTTGTGG ATGTCGTCGT GGGCTTTGAC ATCTCATCTC AACAGAAAGG GCAGCCTTTA 
TTTCAAGGTC AGGCTTGGAT GAAGACTTAT CTTCAGGACG TGTTACGTGA CATCAGTTCC 
CTAAATGGGG TGAGCTGTGA AGTGGGATCT CAGACTCAGG TCAGCGTGGC CTTTCAAGTG 
ACAAATGCCA TGGATAAATT TTCCCCCAAG TTTGAGATTT ACAGTGAAAG TATCCTGAAC 
TCCTTGAAGA ATGTATCCAT TAAGGAGCCG TCTCGCCTCA ATGCACCTTT CTTGAATTCA 
CTTTGGGATG CCTTTCAGAA TAAATCTACT ACTCGAGGAA AGGTGCTGCT ATTATTTTCA 
GATGGATTGG ATGATGATAT TGAAGTACTT GAACAAAAAT CTGATCAACT TCGAAAAGAA 
GGGCTCAACG CTTTGATAAC CGTTGCTCTG GAAGAAGCTA CTGATGCAAA TGACCTAGCT 
GATCTTCTGT ACATTGAATT TGGGAAAGGA TTTGATTATA GGACTCAGTT AACAATCGGC 
ATGAGAGATT TGGGAAGCAG GCTCTCAAAA TATCTGGCTA ATGTTGCTGA AAGGACATGT 
TGCTGTTTGC TTTGCAAGTG TTCTGGGGGA GATGGTGCCG TTGGAGTACC AGGCACACCA 
GGGAAGAAGG GACTTCTAGG TTTTAAAGGC AGTGAAGGCC ACTTGGGAGA AGAAGGTGTC 
CCTGGAGAAA GAGGCTCCCC TGGACCAGTG GGAGAGGAAG GCATTAAAGG ATGCCATGGG 
AGCAAAGGGC CCAAGGGAAA CAGGGGACTC AGTGGAGAGG AGGGAGAAAT TGGGGAACTC 
GGGATTAATG GGATCAATGG AGAACAGGGT GAAGGCGGCC TTCCAGGAAC AAAAGGAGAA 
AAAGGAGATG AAGGTTCCCA GGGCAACCCT GGGAAGAAAG GACCCCCAGG TGACAGTGGC 
CAAAGGGGAG TGCGAGGAGA TCCTGGCAAC CCTGGATCAG ACAATAACAG AAATGGACTC 
ACGGGCCCAA AAGGAGAAAG GGGAAGACAA GGAAGGCGTG GACAGCCAGG GACACCAGGA 
TCTCCGGGCT CCCGAGGAGA CACACCAATA CATGGCCGAC GAGGACCAAG AGGCCCACAG 
GGGATAAATG GAATTTCTGG TCTGGTTGGT CATCAAGGAG CATTGGGATT AAAAGGACCT 
GAGGGCCCAA GAGGAGATCC TGGCACAAAA GGAGCAAAAG GAAATCTAGG AAATAAAGGC 
CCCTCGGGTC CTCCAGGACC AAATGGTGGT AAAGGGAGTC CTGGCAGCCT GGGGAGAAGA 
GGAAATAAGG GAGAGCCAGG GTCTCCAGGA GAGAAAGGAG CAGTTGGATC ATCTGGATAT 
CGTGGAATGC CGGGAGAGGA TGGAAGCGCA GGGTATGGCA GAAGTGGAAG AAAAGGAGAA 
AAGGGACAAG AAGGATTCCC AGGAGATAGT GGACCAAAGG GGGAAGTTGG TGATCCTGGA 
AGGCCAGGAG AAGTTGGACC AAAAGGATAT AAAGGAAGAA CGAGCCCTTC AGGACCACTG 
GGGGAACCAG GATCTCCTGG ATCCCATGGA CCTTCAGGAC AGAAGGGCCC AAAAGGAGCA 
AAGGGTTGGA CTTCATTTTC GACCTGTGAT GTCATTGAGT TTATACGGGA CCATAGCCCT 
TGCTGGAAGG GAAAGCCGGA ATGTCCCGTC CATCCCACCG AGTTGGTGTT TGCTTTGGAT 
CTGTCCCGAG ACGTGTCGGA GGAGGAGTTC GATCGAATGA AGGAGATGAC TATTGCAATT 
GTGAACGACC TCCGAATCAG GGAAGGCAAC TGCCCTGTGG GGGCCCGGGT GGCTGTGGTC 
TCCTACAACT CCAAGACCCG GCATCTCCTC CGGCTGTCTG ACTCCCGGGA CAAGAGCCAG 
CTGCTGCAGG AGATTAAAGC TCTCCGTTTT GAGCCCTCCT CAGGGGAGAG GGAGATTGGC 
AAGACCATGA GGTTCGTGGC CCGGAACGTC TTCAAGAGAA CCCTTCCCGG GGCCAACACA 
AGGAAGATCA CCACCGTTTT CAGCGCTGAC AGGTCCGTGG ACGCGTCCTC CATCGTCACG 
GCCACCATGG AATTCAACGC TCTCGATGTC ATTCCCGTCG TGATTGCTTT TAACAACGTG 
CCTGCCGTCA AGCGCGCTTT CACGATTGAT GACACCGGCA CCTTTCAGGT GCTGACCATC 
CCCCCGAGGG CCGACTACCG GCCGATGCTG AGGAGACTCC AGCAGTGCAC GTTCTGCTAC 
GACAGATGCA AGCCAGAAGT TTCCTGTGAG GAGGCGAAAC CCCCAGCGGT CCCATCCTAT 
ATAGATGCGG CCTTCCTTTT CCAAGGTTCC CGAGATGTGG GAAGCGCCGA GTTTGAGGAT 
GTGAAGGCTT TCCTGAAAGC AGTCTTGGAC AACTTTGACG TGGCCTTTGA ACCCGAGACC 
TCTGCCGTTG GGGATCGTGT TGCACTGCTG AGTCATGCTC CTCCGGGCTT TGTCCCAAAT 
AGCCAGTCAA GCCCGGTCCG GACGGAGTTC AACTTCACCA CCTACAACAA TAAACGCCTG 
ATGAAGAGAC ATGTGGAAGA ATCGGTTCAA CAGCTAAACG GGGAAGCTTT CTTGGGCCAT 
GCCCTGCAGT GGACAGTGGA CAATATCTTT ACAGGCACCC CCAACCTGAG AAAAAACAGA 
GTCATTTTTG TGGTATCTGC TGGAGAAACA AGTTATTTGG ACAAGAAAGT CTTGAAGAGA 
GAATCTCTGC GGGCCAAATG TCAAGGTTAC GCCATATTTG TGTTCTCTCT TGGCTTCTCA 
CACAATGACG AGGAGCTCGA AGAATTAGCA AGCCTCCCCC TCGATCACCA CTTGGTCCAA 
CTTGGCCGAA TTCAGAAACC AGACCATGGC TACGGGGTGA AGTTTGTGAA ATCCTTTATA 
AACTCCGTCA GACGTGCAAT CAACAAGTAT CCGCCAATAA ACTTTAAAGC AAAATGCAAT 
AAAATCAATC TTCCGGAACC GGTGCAGCCT TCCAAACGGC AAAGCCACCT CCTTGCCCGT 
ATTGGACCAC TCGAAGAGAG ATTTCTGTCC AAAAAGCTTC TCTCAAGAGT AACACGGGGG 
AATGCTGTTT GGAACATTCG TGGTTATACC AGGAATATGT CCCGTCCCCT TAAAACTGGA 
AACAGAATGC CACGCATTGT TCCCAAGACT CATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_023505163.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023360931.1 Sarcophilus harrisii collagen type VI alpha 6 chain (COL6A6), mRNA.

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CloneID	OSa156540
Clone ID Related Accession (Same CDS sequence)	XM_023505163.1 , XM_023505163.2
Accession Version	XM_023505163.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	collagen alpha-6(VI) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045430.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_023505163.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTGT TTATCTTCCT CATCATAACT TGTGTCCAAG CACAGTCTGT GACCCAGGAT 
GTTGGTCCTG AGTTTGCTGA TGTTGTGTTC CTGGTGGATA GCTCTGATCA CCTAGCGAGT 
AAGTTCTCTT TTGTGAGAAC CTTCATCTCC AAGACGATCA ACAGTCTGCC CATTGAAGCC 
AACAAGTTCC GTATTGCCCT CGCCCAGTAT AGCGATGATC TGCGCCAGGA GTTCCTGCTG 
AGCCCTTTCA TGGCCAAGGG CGCCATATTG AATTACATCA AGAGGAACTT CACCAATGTT 
GGAGGATCTT TGCGAATAGG CAACGCTCTC CAGAAAGTCC ACAAGACCTA CTTCTCTGGG 
CCAGTCAGTG GAAGAGACAA GAGCCAGTTT CCCCAGATTC TGGTAGTTCT GGCTTCAGCC 
CACTCTGAGG ATGATGTAAA GGGGCCAGCC AACGCTCTGC AGAGAGATGG GGTGAAAATC 
ATCTCACTGG GGATGCAGAA TGCTTCGGAA GAAAACCTAA AAGCCATGGC CACATCTAAG 
TTTCATTACA ACCTAAGAAC AGTGAGAGAT GTGGCCACAT TTTCCACAAA TATGACCAAT 
ATCATTAAAG AAGTGGTAAA ATACAAGGGA GCAGATGATG CTGATGTGGA AGTTTGTCAT 
GGCACTTCTG TGGCTGATGT CGTATTTCTC CTGGACAACT ATGTCAATGG CACCAAGGAA 
AATTTGGAAT ATCTTAAAAG TTTCCTAGAA GAGAGCGTGT CCTCCTTTGA TGTGAAAGAG 
AGCTGTATGA GGATTGGCCT AGTGACCTAC AGCAATGAGA CAAAGGTGAT TCGTAACCTG 
GGCACTGGGA TCAACAAGTC AGAGATTCTG CAAGAGATAA AGAACCTATC TCCCAGAGCT 
GGGAGAGCTC GTGCTGGAGC CGCCATCACA AAGCTCAGGA AAGAGGTCTT CAATGTCCGC 
AGAGGCAGCC GAAAGATGCA GGGGGTCCCT CAAATAGCCA TTCTAGTCAC TCACAGACCA 
TCAGAAGATA ATGTTTCTGT AGCTGCACTT GACCTTCGGC GTGAAGGCGT GACTGTGTTC 
ACTGTGGGCC TGGAAGGTTC TAATTATACC CAGTTAGAAC AAATCTCATC CTACCCTCCA 
GAAAAGTATA TTTCCAAACT GAAAACATTT TCAGACTTGG CAGCCCATAA TCAGACATTT 
CGGAAGAAAC TCAGGAATGA AATACAGTAC AAGGTATCCA TCCTGTCAGA ACGGACCGAA 
CACCTGAAAT CTGGTTGTGT GGAAACTGAA GACGCGGACA TTTATTTACT CATTGATGGC 
TCAGGAAGTA TCCACCCAAC AGACTTCCAG GAAATGAAAA CATTTTTGTC AGAAGTGATA 
GGAATGCTCA GCATAGCACC CCATAAAGTC AGAGTTGGAG CCGTTCAGTA TTCCGACAGA 
TGGCAACTGG AATTTGAGAT CAACCAATAC TCAAACAAAA ATGACTTGGG CAAGGCTATT 
GACAATATCC GGCAGATGGG AGGCAACACC AACACAGGCG CAGCCCTGGA TTTTACTCTT 
GGGCTTCTCC AAGAAGCAAA GCGTCAACGA GGAAACAAGG TACCATGTCA TCTGATTGTT 
TTGACAGATG GGTTGTCAGA TGATCCCGTT AAGGACCCTT CCAAGAAACT GCATGATGAG 
CACATCGATG TGTTTGCCAT TGGTGTCAAG GAGGCAAATC GAACTCAGTT GCTAGAAATT 
GCTGGGTCAG AAAAGAGAGT TTACTATGTA TATAACTTTG ATTCTTTGAA AGACATAAAA 
AATGAAGTTG TTCAAGGAAT CTGTTCTGAA GAAGCTTGTA AAGATATGAG ATCCGATATC 
ATGTTTCTCG TGGATAGTTC TGGGAGTATA GGAGCTGAAA ATTTCGCTAA AATGAAGACG 
TTTATGAAAA ACCTGGTGAG CAAGTCTCAG ATTGGAGCAG ACCAAGTACA AATTGGCGTC 
GTCCAGTTCA GCGATGTGAA CAGGGAAGAA TTTCAGCTCA ATAGATATAG GACCCAACGT 
GAAATTGCTG ATGCAATAGA TAGAATGCCT CTCCTAGACC AGACAACCAT GACGGGAAGT 
GCTCTGACCT TTGTCTCTGA CTACTTCCAT ACCTCCAAAG GGGCACGTCC CAATGTCAAA 
AAGTTTCTCA TCCTCATCAC AGATGGAGAG GCTCAGGACC TGGTCAAGGA CCCAGCTCTG 
GCTCTTCGGC AAGATGGAAT AATTATCTAC TCTGTGGGAG TGTATGGATC AAATCAAACC 
CAGCTAGTGG AAATCAGTGG GAGGCGTGAT TTGGTTTTTT ATGTGGAGAA TTTTGATATT 
CTAAAGCGCA TAGAAGATCA ACTCATTTTT GGCATATGTA GCCCCCATGA AGAATGCAAA 
CAGATAGAAG TGTTAGATGT AGTATTCATC ATTGACAGCT CTGGCAGCAT AGCCCCGGAT 
GACTATAACA TCATGAAGAA CTTCATGATT GAGCTGGTGA AAAAAGCCGA TGTTGGCAAG 
AACCGAGTTC AGTTTGGAGC CCTGAAATAC TCTAATGAGC CCGAGATAAT TTTTTATCTT 
GATGAACATG ACACAAAGTC CAAGGTGATT TCGGTTCTCC AGAGTGATTC TCCCCTAGGA 
GGAGACACGT ACACTGCCAA AGCCCTGGCC TTTTCGGAGC ACATGTTCAC CGAGCCCCGG 
GGCAGCCGCA TTCACAGAAG GGTCCCCCAG GTCCTCATCG TGATCACAGA TGGGGTATCC 
CACGACGCAG ACAAGCTCAA CGCCACGGCC AAAGCACTAA GGGACAAAGG AATCCTCATC 
CTGGCAGTGG GCATCAAAGG AGCCAACACT AAGGAGCTGT TGGCAATAGC CGGATCGGAA 
GACAAGTATT TCTTTGTGGA AACCTTCGGG GGCCTGAAGG GCATATTCTC AAATGTGTCA 
GACAGTATCT GTGCTCCTTC AAAAGTAGAT TGTGAAATTG AAAGTGCAGA TCTTGTTTTC 
CTTGTTGATG GTTCTACTAG CATACAACCA CATGACTTCC AGAAAATGAA GGATTTTTTA 
GTATCTGTAG TAGATGATTT TGACATCAGA CCTAGCAAAG TGCATGTTGG ACTGGCTCAA 
TTCAGTGATA GCTACAAGTC TGAGTTTTAT CTGGGGAAAT TCACAACGGA AAAGGAAGTT 
TCTGCTCAAA TTGAAAGGAT CTACCAGATC TTTGGAAATA CCCACATTGG AGCTGCGCTG 
AGGCAAGTGG AGGAATATTT CCGACCAGAG ATGGGCAGCA GAATAAATAG AGGCACCCAG 
CAGGTTTTAT TGGTCCTCAC TGATGGGCAA TCCCAAGATG AGTTGGCTAA GGCTGCTGAA 
GACCTAAGAA GGAAAGGAAT TGATATATAC TCTTTGGGCA TTGGGGATGT AGATGAGCAG 
CAGCTCATTC AGATAACTGG AACATCGGAG AAAAAACTAA CGGTCGATAA CTTTGACGAG 
CTGAAAAAGA TCAAAAAAAG GATAGTCCGC AACATCTGTA CATCAGGCGG TGAAAGCAGT 
TGTTTTGTGG ATGTCGTCGT GGGCTTTGAC ATCTCATCTC AACAGAAAGG GCAGCCTTTA 
TTTCAAGGTC AGGCTTGGAT GAAGACTTAT CTTCAGGACG TGTTACGTGA CATCAGTTCC 
CTAAATGGGG TGAGCTGTGA AGTGGGATCT CAGACTCAGG TCAGCGTGGC CTTTCAAGTG 
ACAAATGCCA TGGATAAATT TTCCCCCAAG TTTGAGATTT ACAGTGAAAG TATCCTGAAC 
TCCTTGAAGA ATGTATCCAT TAAGGAGCCG TCTCGCCTCA ATGCACCTTT CTTGAATTCA 
CTTTGGGATG CCTTTCAGAA TAAATCTACT ACTCGAGGAA AGGTGCTGCT ATTATTTTCA 
GATGGATTGG ATGATGATAT TGAAGTACTT GAACAAAAAT CTGATCAACT TCGAAAAGAA 
GGGCTCAACG CTTTGATAAC CGTTGCTCTG GAAGAAGCTA CTGATGCAAA TGACCTAGCT 
GATCTTCTGT ACATTGAATT TGGGAAAGGA TTTGATTATA GGACTCAGTT AACAATCGGC 
ATGAGAGATT TGGGAAGCAG GCTCTCAAAA TATCTGGCTA ATGTTGCTGA AAGGACATGT 
TGCTGTTTGC TTTGCAAGTG TTCTGGGGGA GATGGTGCCG TTGGAGTACC AGGCACACCA 
GGGAAGAAGG GACTTCTAGG TTTTAAAGGC AGTGAAGGCC ACTTGGGAGA AGAAGGTGTC 
CCTGGAGAAA GAGGCTCCCC TGGACCAGTG GGAGAGGAAG GCATTAAAGG ATGCCATGGG 
AGCAAAGGGC CCAAGGGAAA CAGGGGACTC AGTGGAGAGG AGGGAGAAAT TGGGGAACTC 
GGGATTAATG GGATCAATGG AGAACAGGGT GAAGGCGGCC TTCCAGGAAC AAAAGGAGAA 
AAAGGAGATG AAGGTTCCCA GGGCAACCCT GGGAAGAAAG GACCCCCAGG TGACAGTGGC 
CAAAGGGGAG TGCGAGGAGA TCCTGGCAAC CCTGGATCAG ACAATAACAG AAATGGACTC 
ACGGGCCCAA AAGGAGAAAG GGGAAGACAA GGAAGGCGTG GACAGCCAGG GACACCAGGA 
TCTCCGGGCT CCCGAGGAGA CACACCAATA CATGGCCGAC GAGGACCAAG AGGCCCACAG 
GGGATAAATG GAATTTCTGG TCTGGTTGGT CATCAAGGAG CATTGGGATT AAAAGGACCT 
GAGGGCCCAA GAGGAGATCC TGGCACAAAA GGAGCAAAAG GAAATCTAGG AAATAAAGGC 
CCCTCGGGTC CTCCAGGACC AAATGGTGGT AAAGGGAGTC CTGGCAGCCT GGGGAGAAGA 
GGAAATAAGG GAGAGCCAGG GTCTCCAGGA GAGAAAGGAG CAGTTGGATC ATCTGGATAT 
CGTGGAATGC CGGGAGAGGA TGGAAGCGCA GGGTATGGCA GAAGTGGAAG AAAAGGAGAA 
AAGGGACAAG AAGGATTCCC AGGAGATAGT GGACCAAAGG GGGAAGTTGG TGATCCTGGA 
AGGCCAGGAG AAGTTGGACC AAAAGGATAT AAAGGAAGAA CGAGCCCTTC AGGACCACTG 
GGGGAACCAG GATCTCCTGG ATCCCATGGA CCTTCAGGAC AGAAGGGCCC AAAAGGAGCA 
AAGGGTTGGA CTTCATTTTC GACCTGTGAT GTCATTGAGT TTATACGGGA CCATAGCCCT 
TGCTGGAAGG GAAAGCCGGA ATGTCCCGTC CATCCCACCG AGTTGGTGTT TGCTTTGGAT 
CTGTCCCGAG ACGTGTCGGA GGAGGAGTTC GATCGAATGA AGGAGATGAC TATTGCAATT 
GTGAACGACC TCCGAATCAG GGAAGGCAAC TGCCCTGTGG GGGCCCGGGT GGCTGTGGTC 
TCCTACAACT CCAAGACCCG GCATCTCCTC CGGCTGTCTG ACTCCCGGGA CAAGAGCCAG 
CTGCTGCAGG AGATTAAAGC TCTCCGTTTT GAGCCCTCCT CAGGGGAGAG GGAGATTGGC 
AAGACCATGA GGTTCGTGGC CCGGAACGTC TTCAAGAGAA CCCTTCCCGG GGCCAACACA 
AGGAAGATCA CCACCGTTTT CAGCGCTGAC AGGTCCGTGG ACGCGTCCTC CATCGTCACG 
GCCACCATGG AATTCAACGC TCTCGATGTC ATTCCCGTCG TGATTGCTTT TAACAACGTG 
CCTGCCGTCA AGCGCGCTTT CACGATTGAT GACACCGGCA CCTTTCAGGT GCTGACCATC 
CCCCCGAGGG CCGACTACCG GCCGATGCTG AGGAGACTCC AGCAGTGCAC GTTCTGCTAC 
GACAGATGCA AGCCAGAAGT TTCCTGTGAG GAGGCGAAAC CCCCAGCGGT CCCATCCTAT 
ATAGATGCGG CCTTCCTTTT CCAAGGTTCC CGAGATGTGG GAAGCGCCGA GTTTGAGGAT 
GTGAAGGCTT TCCTGAAAGC AGTCTTGGAC AACTTTGACG TGGCCTTTGA ACCCGAGACC 
TCTGCCGTTG GGGATCGTGT TGCACTGCTG AGTCATGCTC CTCCGGGCTT TGTCCCAAAT 
AGCCAGTCAA GCCCGGTCCG GACGGAGTTC AACTTCACCA CCTACAACAA TAAACGCCTG 
ATGAAGAGAC ATGTGGAAGA ATCGGTTCAA CAGCTAAACG GGGAAGCTTT CTTGGGCCAT 
GCCCTGCAGT GGACAGTGGA CAATATCTTT ACAGGCACCC CCAACCTGAG AAAAAACAGA 
GTCATTTTTG TGGTATCTGC TGGAGAAACA AGTTATTTGG ACAAGAAAGT CTTGAAGAGA 
GAATCTCTGC GGGCCAAATG TCAAGGTTAC GCCATATTTG TGTTCTCTCT TGGCTTCTCA 
CACAATGACG AGGAGCTCGA AGAATTAGCA AGCCTCCCCC TCGATCACCA CTTGGTCCAA 
CTTGGCCGAA TTCAGAAACC AGACCATGGC TACGGGGTGA AGTTTGTGAA ATCCTTTATA 
AACTCCGTCA GACGTGCAAT CAACAAGTAT CCGCCAATAA ACTTTAAAGC AAAATGCAAT 
AAAATCAATC TTCCGGAACC GGTGCAGCCT TCCAAACGGC AAAGCCACCT CCTTGCCCGT 
ATTGGACCAC TCGAAGAGAG ATTTCTGTCC AAAAAGCTTC TCTCAAGAGT AACACGGGGG 
AATGCTGTTT GGAACATTCG TGGTTATACC AGGAATATGT CCCGTCCCCT TAAAACTGGA 
AACAGAATGC CACGCATTGT TCCCAAGACT CATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023360931.1
CDS	396..7154
Translation	MFLFIFLIITCVQAQSVTQDVGPEFADVVFLVDSSDHLASKFSFVRTFISKTINSLPIEANKFRIALAQYSDDLRQEFLLSPFMAKGAILNYIKRNFTNVGGSLRIGNALQKVHKTYFSGPVSGRDKSQFPQILVVLASAHSEDDVKGPANALQRDGVKIISLGMQNASEENLKAMATSKFHYNLRTVRDVATFSTNMTNIIKEVVKYKGADDADVEVCHGTSVADVVFLLDNYVNGTKENLEYLKSFLEESVSSFDVKESCMRIGLVTYSNETKVIRNLGTGINKSEILQEIKNLSPRAGRARAGAAITKLRKEVFNVRRGSRKMQGVPQIAILVTHRPSEDNVSVAALDLRREGVTVFTVGLEGSNYTQLEQISSYPPEKYISKLKTFSDLAAHNQTFRKKLRNEIQYKVSILSERTEHLKSGCVETEDADIYLLIDGSGSIHPTDFQEMKTFLSEVIGMLSIAPHKVRVGAVQYSDRWQLEFEINQYSNKNDLGKAIDNIRQMGGNTNTGAALDFTLGLLQEAKRQRGNKVPCHLIVLTDGLSDDPVKDPSKKLHDEHIDVFAIGVKEANRTQLLEIAGSEKRVYYVYNFDSLKDIKNEVVQGICSEEACKDMRSDIMFLVDSSGSIGAENFAKMKTFMKNLVSKSQIGADQVQIGVVQFSDVNREEFQLNRYRTQREIADAIDRMPLLDQTTMTGSALTFVSDYFHTSKGARPNVKKFLILITDGEAQDLVKDPALALRQDGIIIYSVGVYGSNQTQLVEISGRRDLVFYVENFDILKRIEDQLIFGICSPHEECKQIEVLDVVFIIDSSGSIAPDDYNIMKNFMIELVKKADVGKNRVQFGALKYSNEPEIIFYLDEHDTKSKVISVLQSDSPLGGDTYTAKALAFSEHMFTEPRGSRIHRRVPQVLIVITDGVSHDADKLNATAKALRDKGILILAVGIKGANTKELLAIAGSEDKYFFVETFGGLKGIFSNVSDSICAPSKVDCEIESADLVFLVDGSTSIQPHDFQKMKDFLVSVVDDFDIRPSKVHVGLAQFSDSYKSEFYLGKFTTEKEVSAQIERIYQIFGNTHIGAALRQVEEYFRPEMGSRINRGTQQVLLVLTDGQSQDELAKAAEDLRRKGIDIYSLGIGDVDEQQLIQITGTSEKKLTVDNFDELKKIKKRIVRNICTSGGESSCFVDVVVGFDISSQQKGQPLFQGQAWMKTYLQDVLRDISSLNGVSCEVGSQTQVSVAFQVTNAMDKFSPKFEIYSESILNSLKNVSIKEPSRLNAPFLNSLWDAFQNKSTTRGKVLLLFSDGLDDDIEVLEQKSDQLRKEGLNALITVALEEATDANDLADLLYIEFGKGFDYRTQLTIGMRDLGSRLSKYLANVAERTCCCLLCKCSGGDGAVGVPGTPGKKGLLGFKGSEGHLGEEGVPGERGSPGPVGEEGIKGCHGSKGPKGNRGLSGEEGEIGELGINGINGEQGEGGLPGTKGEKGDEGSQGNPGKKGPPGDSGQRGVRGDPGNPGSDNNRNGLTGPKGERGRQGRRGQPGTPGSPGSRGDTPIHGRRGPRGPQGINGISGLVGHQGALGLKGPEGPRGDPGTKGAKGNLGNKGPSGPPGPNGGKGSPGSLGRRGNKGEPGSPGEKGAVGSSGYRGMPGEDGSAGYGRSGRKGEKGQEGFPGDSGPKGEVGDPGRPGEVGPKGYKGRTSPSGPLGEPGSPGSHGPSGQKGPKGAKGWTSFSTCDVIEFIRDHSPCWKGKPECPVHPTELVFALDLSRDVSEEEFDRMKEMTIAIVNDLRIREGNCPVGARVAVVSYNSKTRHLLRLSDSRDKSQLLQEIKALRFEPSSGEREIGKTMRFVARNVFKRTLPGANTRKITTVFSADRSVDASSIVTATMEFNALDVIPVVIAFNNVPAVKRAFTIDDTGTFQVLTIPPRADYRPMLRRLQQCTFCYDRCKPEVSCEEAKPPAVPSYIDAAFLFQGSRDVGSAEFEDVKAFLKAVLDNFDVAFEPETSAVGDRVALLSHAPPGFVPNSQSSPVRTEFNFTTYNNKRLMKRHVEESVQQLNGEAFLGHALQWTVDNIFTGTPNLRKNRVIFVVSAGETSYLDKKVLKRESLRAKCQGYAIFVFSLGFSHNDEELEELASLPLDHHLVQLGRIQKPDHGYGVKFVKSFINSVRRAINKYPPINFKAKCNKINLPEPVQPSKRQSHLLARIGPLEERFLSKKLLSRVTRGNAVWNIRGYTRNMSRPLKTGNRMPRIVPKTHL

Target ORF information:

RefSeq Version	XM_023505163.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii collagen type VI alpha 6 chain (COL6A6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023505163.2

ORF Insert Sequence:

ATGTTCCTGT TTATCTTCCT CATCATAACT TGTGTCCAAG CACAGTCTGT GACCCAGGAT 
GTTGGTCCTG AGTTTGCTGA TGTTGTGTTC CTGGTGGATA GCTCTGATCA CCTAGCGAGT 
AAGTTCTCTT TTGTGAGAAC CTTCATCTCC AAGACGATCA ACAGTCTGCC CATTGAAGCC 
AACAAGTTCC GTATTGCCCT CGCCCAGTAT AGCGATGATC TGCGCCAGGA GTTCCTGCTG 
AGCCCTTTCA TGGCCAAGGG CGCCATATTG AATTACATCA AGAGGAACTT CACCAATGTT 
GGAGGATCTT TGCGAATAGG CAACGCTCTC CAGAAAGTCC ACAAGACCTA CTTCTCTGGG 
CCAGTCAGTG GAAGAGACAA GAGCCAGTTT CCCCAGATTC TGGTAGTTCT GGCTTCAGCC 
CACTCTGAGG ATGATGTAAA GGGGCCAGCC AACGCTCTGC AGAGAGATGG GGTGAAAATC 
ATCTCACTGG GGATGCAGAA TGCTTCGGAA GAAAACCTAA AAGCCATGGC CACATCTAAG 
TTTCATTACA ACCTAAGAAC AGTGAGAGAT GTGGCCACAT TTTCCACAAA TATGACCAAT 
ATCATTAAAG AAGTGGTAAA ATACAAGGGA GCAGATGATG CTGATGTGGA AGTTTGTCAT 
GGCACTTCTG TGGCTGATGT CGTATTTCTC CTGGACAACT ATGTCAATGG CACCAAGGAA 
AATTTGGAAT ATCTTAAAAG TTTCCTAGAA GAGAGCGTGT CCTCCTTTGA TGTGAAAGAG 
AGCTGTATGA GGATTGGCCT AGTGACCTAC AGCAATGAGA CAAAGGTGAT TCGTAACCTG 
GGCACTGGGA TCAACAAGTC AGAGATTCTG CAAGAGATAA AGAACCTATC TCCCAGAGCT 
GGGAGAGCTC GTGCTGGAGC CGCCATCACA AAGCTCAGGA AAGAGGTCTT CAATGTCCGC 
AGAGGCAGCC GAAAGATGCA GGGGGTCCCT CAAATAGCCA TTCTAGTCAC TCACAGACCA 
TCAGAAGATA ATGTTTCTGT AGCTGCACTT GACCTTCGGC GTGAAGGCGT GACTGTGTTC 
ACTGTGGGCC TGGAAGGTTC TAATTATACC CAGTTAGAAC AAATCTCATC CTACCCTCCA 
GAAAAGTATA TTTCCAAACT GAAAACATTT TCAGACTTGG CAGCCCATAA TCAGACATTT 
CGGAAGAAAC TCAGGAATGA AATACAGTAC AAGGTATCCA TCCTGTCAGA ACGGACCGAA 
CACCTGAAAT CTGGTTGTGT GGAAACTGAA GACGCGGACA TTTATTTACT CATTGATGGC 
TCAGGAAGTA TCCACCCAAC AGACTTCCAG GAAATGAAAA CATTTTTGTC AGAAGTGATA 
GGAATGCTCA GCATAGCACC CCATAAAGTC AGAGTTGGAG CCGTTCAGTA TTCCGACAGA 
TGGCAACTGG AATTTGAGAT CAACCAATAC TCAAACAAAA ATGACTTGGG CAAGGCTATT 
GACAATATCC GGCAGATGGG AGGCAACACC AACACAGGCG CAGCCCTGGA TTTTACTCTT 
GGGCTTCTCC AAGAAGCAAA GCGTCAACGA GGAAACAAGG TACCATGTCA TCTGATTGTT 
TTGACAGATG GGTTGTCAGA TGATCCCGTT AAGGACCCTT CCAAGAAACT GCATGATGAG 
CACATCGATG TGTTTGCCAT TGGTGTCAAG GAGGCAAATC GAACTCAGTT GCTAGAAATT 
GCTGGGTCAG AAAAGAGAGT TTACTATGTA TATAACTTTG ATTCTTTGAA AGACATAAAA 
AATGAAGTTG TTCAAGGAAT CTGTTCTGAA GAAGCTTGTA AAGATATGAG ATCCGATATC 
ATGTTTCTCG TGGATAGTTC TGGGAGTATA GGAGCTGAAA ATTTCGCTAA AATGAAGACG 
TTTATGAAAA ACCTGGTGAG CAAGTCTCAG ATTGGAGCAG ACCAAGTACA AATTGGCGTC 
GTCCAGTTCA GCGATGTGAA CAGGGAAGAA TTTCAGCTCA ATAGATATAG GACCCAACGT 
GAAATTGCTG ATGCAATAGA TAGAATGCCT CTCCTAGACC AGACAACCAT GACGGGAAGT 
GCTCTGACCT TTGTCTCTGA CTACTTCCAT ACCTCCAAAG GGGCACGTCC CAATGTCAAA 
AAGTTTCTCA TCCTCATCAC AGATGGAGAG GCTCAGGACC TGGTCAAGGA CCCAGCTCTG 
GCTCTTCGGC AAGATGGAAT AATTATCTAC TCTGTGGGAG TGTATGGATC AAATCAAACC 
CAGCTAGTGG AAATCAGTGG GAGGCGTGAT TTGGTTTTTT ATGTGGAGAA TTTTGATATT 
CTAAAGCGCA TAGAAGATCA ACTCATTTTT GGCATATGTA GCCCCCATGA AGAATGCAAA 
CAGATAGAAG TGTTAGATGT AGTATTCATC ATTGACAGCT CTGGCAGCAT AGCCCCGGAT 
GACTATAACA TCATGAAGAA CTTCATGATT GAGCTGGTGA AAAAAGCCGA TGTTGGCAAG 
AACCGAGTTC AGTTTGGAGC CCTGAAATAC TCTAATGAGC CCGAGATAAT TTTTTATCTT 
GATGAACATG ACACAAAGTC CAAGGTGATT TCGGTTCTCC AGAGTGATTC TCCCCTAGGA 
GGAGACACGT ACACTGCCAA AGCCCTGGCC TTTTCGGAGC ACATGTTCAC CGAGCCCCGG 
GGCAGCCGCA TTCACAGAAG GGTCCCCCAG GTCCTCATCG TGATCACAGA TGGGGTATCC 
CACGACGCAG ACAAGCTCAA CGCCACGGCC AAAGCACTAA GGGACAAAGG AATCCTCATC 
CTGGCAGTGG GCATCAAAGG AGCCAACACT AAGGAGCTGT TGGCAATAGC CGGATCGGAA 
GACAAGTATT TCTTTGTGGA AACCTTCGGG GGCCTGAAGG GCATATTCTC AAATGTGTCA 
GACAGTATCT GTGCTCCTTC AAAAGTAGAT TGTGAAATTG AAAGTGCAGA TCTTGTTTTC 
CTTGTTGATG GTTCTACTAG CATACAACCA CATGACTTCC AGAAAATGAA GGATTTTTTA 
GTATCTGTAG TAGATGATTT TGACATCAGA CCTAGCAAAG TGCATGTTGG ACTGGCTCAA 
TTCAGTGATA GCTACAAGTC TGAGTTTTAT CTGGGGAAAT TCACAACGGA AAAGGAAGTT 
TCTGCTCAAA TTGAAAGGAT CTACCAGATC TTTGGAAATA CCCACATTGG AGCTGCGCTG 
AGGCAAGTGG AGGAATATTT CCGACCAGAG ATGGGCAGCA GAATAAATAG AGGCACCCAG 
CAGGTTTTAT TGGTCCTCAC TGATGGGCAA TCCCAAGATG AGTTGGCTAA GGCTGCTGAA 
GACCTAAGAA GGAAAGGAAT TGATATATAC TCTTTGGGCA TTGGGGATGT AGATGAGCAG 
CAGCTCATTC AGATAACTGG AACATCGGAG AAAAAACTAA CGGTCGATAA CTTTGACGAG 
CTGAAAAAGA TCAAAAAAAG GATAGTCCGC AACATCTGTA CATCAGGCGG TGAAAGCAGT 
TGTTTTGTGG ATGTCGTCGT GGGCTTTGAC ATCTCATCTC AACAGAAAGG GCAGCCTTTA 
TTTCAAGGTC AGGCTTGGAT GAAGACTTAT CTTCAGGACG TGTTACGTGA CATCAGTTCC 
CTAAATGGGG TGAGCTGTGA AGTGGGATCT CAGACTCAGG TCAGCGTGGC CTTTCAAGTG 
ACAAATGCCA TGGATAAATT TTCCCCCAAG TTTGAGATTT ACAGTGAAAG TATCCTGAAC 
TCCTTGAAGA ATGTATCCAT TAAGGAGCCG TCTCGCCTCA ATGCACCTTT CTTGAATTCA 
CTTTGGGATG CCTTTCAGAA TAAATCTACT ACTCGAGGAA AGGTGCTGCT ATTATTTTCA 
GATGGATTGG ATGATGATAT TGAAGTACTT GAACAAAAAT CTGATCAACT TCGAAAAGAA 
GGGCTCAACG CTTTGATAAC CGTTGCTCTG GAAGAAGCTA CTGATGCAAA TGACCTAGCT 
GATCTTCTGT ACATTGAATT TGGGAAAGGA TTTGATTATA GGACTCAGTT AACAATCGGC 
ATGAGAGATT TGGGAAGCAG GCTCTCAAAA TATCTGGCTA ATGTTGCTGA AAGGACATGT 
TGCTGTTTGC TTTGCAAGTG TTCTGGGGGA GATGGTGCCG TTGGAGTACC AGGCACACCA 
GGGAAGAAGG GACTTCTAGG TTTTAAAGGC AGTGAAGGCC ACTTGGGAGA AGAAGGTGTC 
CCTGGAGAAA GAGGCTCCCC TGGACCAGTG GGAGAGGAAG GCATTAAAGG ATGCCATGGG 
AGCAAAGGGC CCAAGGGAAA CAGGGGACTC AGTGGAGAGG AGGGAGAAAT TGGGGAACTC 
GGGATTAATG GGATCAATGG AGAACAGGGT GAAGGCGGCC TTCCAGGAAC AAAAGGAGAA 
AAAGGAGATG AAGGTTCCCA GGGCAACCCT GGGAAGAAAG GACCCCCAGG TGACAGTGGC 
CAAAGGGGAG TGCGAGGAGA TCCTGGCAAC CCTGGATCAG ACAATAACAG AAATGGACTC 
ACGGGCCCAA AAGGAGAAAG GGGAAGACAA GGAAGGCGTG GACAGCCAGG GACACCAGGA 
TCTCCGGGCT CCCGAGGAGA CACACCAATA CATGGCCGAC GAGGACCAAG AGGCCCACAG 
GGGATAAATG GAATTTCTGG TCTGGTTGGT CATCAAGGAG CATTGGGATT AAAAGGACCT 
GAGGGCCCAA GAGGAGATCC TGGCACAAAA GGAGCAAAAG GAAATCTAGG AAATAAAGGC 
CCCTCGGGTC CTCCAGGACC AAATGGTGGT AAAGGGAGTC CTGGCAGCCT GGGGAGAAGA 
GGAAATAAGG GAGAGCCAGG GTCTCCAGGA GAGAAAGGAG CAGTTGGATC ATCTGGATAT 
CGTGGAATGC CGGGAGAGGA TGGAAGCGCA GGGTATGGCA GAAGTGGAAG AAAAGGAGAA 
AAGGGACAAG AAGGATTCCC AGGAGATAGT GGACCAAAGG GGGAAGTTGG TGATCCTGGA 
AGGCCAGGAG AAGTTGGACC AAAAGGATAT AAAGGAAGAA CGAGCCCTTC AGGACCACTG 
GGGGAACCAG GATCTCCTGG ATCCCATGGA CCTTCAGGAC AGAAGGGCCC AAAAGGAGCA 
AAGGGTTGGA CTTCATTTTC GACCTGTGAT GTCATTGAGT TTATACGGGA CCATAGCCCT 
TGCTGGAAGG GAAAGCCGGA ATGTCCCGTC CATCCCACCG AGTTGGTGTT TGCTTTGGAT 
CTGTCCCGAG ACGTGTCGGA GGAGGAGTTC GATCGAATGA AGGAGATGAC TATTGCAATT 
GTGAACGACC TCCGAATCAG GGAAGGCAAC TGCCCTGTGG GGGCCCGGGT GGCTGTGGTC 
TCCTACAACT CCAAGACCCG GCATCTCCTC CGGCTGTCTG ACTCCCGGGA CAAGAGCCAG 
CTGCTGCAGG AGATTAAAGC TCTCCGTTTT GAGCCCTCCT CAGGGGAGAG GGAGATTGGC 
AAGACCATGA GGTTCGTGGC CCGGAACGTC TTCAAGAGAA CCCTTCCCGG GGCCAACACA 
AGGAAGATCA CCACCGTTTT CAGCGCTGAC AGGTCCGTGG ACGCGTCCTC CATCGTCACG 
GCCACCATGG AATTCAACGC TCTCGATGTC ATTCCCGTCG TGATTGCTTT TAACAACGTG 
CCTGCCGTCA AGCGCGCTTT CACGATTGAT GACACCGGCA CCTTTCAGGT GCTGACCATC 
CCCCCGAGGG CCGACTACCG GCCGATGCTG AGGAGACTCC AGCAGTGCAC GTTCTGCTAC 
GACAGATGCA AGCCAGAAGT TTCCTGTGAG GAGGCGAAAC CCCCAGCGGT CCCATCCTAT 
ATAGATGCGG CCTTCCTTTT CCAAGGTTCC CGAGATGTGG GAAGCGCCGA GTTTGAGGAT 
GTGAAGGCTT TCCTGAAAGC AGTCTTGGAC AACTTTGACG TGGCCTTTGA ACCCGAGACC 
TCTGCCGTTG GGGATCGTGT TGCACTGCTG AGTCATGCTC CTCCGGGCTT TGTCCCAAAT 
AGCCAGTCAA GCCCGGTCCG GACGGAGTTC AACTTCACCA CCTACAACAA TAAACGCCTG 
ATGAAGAGAC ATGTGGAAGA ATCGGTTCAA CAGCTAAACG GGGAAGCTTT CTTGGGCCAT 
GCCCTGCAGT GGACAGTGGA CAATATCTTT ACAGGCACCC CCAACCTGAG AAAAAACAGA 
GTCATTTTTG TGGTATCTGC TGGAGAAACA AGTTATTTGG ACAAGAAAGT CTTGAAGAGA 
GAATCTCTGC GGGCCAAATG TCAAGGTTAC GCCATATTTG TGTTCTCTCT TGGCTTCTCA 
CACAATGACG AGGAGCTCGA AGAATTAGCA AGCCTCCCCC TCGATCACCA CTTGGTCCAA 
CTTGGCCGAA TTCAGAAACC AGACCATGGC TACGGGGTGA AGTTTGTGAA ATCCTTTATA 
AACTCCGTCA GACGTGCAAT CAACAAGTAT CCGCCAATAA ACTTTAAAGC AAAATGCAAT 
AAAATCAATC TTCCGGAACC GGTGCAGCCT TCCAAACGGC AAAGCCACCT CCTTGCCCGT 
ATTGGACCAC TCGAAGAGAG ATTTCTGTCC AAAAAGCTTC TCTCAAGAGT AACACGGGGG 
AATGCTGTTT GGAACATTCG TGGTTATACC AGGAATATGT CCCGTCCCCT TAAAACTGGA 
AACAGAATGC CACGCATTGT TCCCAAGACT CATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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COL6A6 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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