HEATR5B cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	HEATR5B
Entrez Gene ID	100917641
Full Name	HEAT repeat containing 5B
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031951270.1	XP_031807130.1	HEAT repeat-containing protein 5B isoform X1
XM_031951271.1	XP_031807131.1	HEAT repeat-containing protein 5B isoform X2
XM_031951272.1	XP_031807132.1	HEAT repeat-containing protein 5B isoform X1
XM_012539862.1	XP_012395316.1	HEAT repeat-containing protein 5B
XM_023494615.1	XP_023350383.1	HEAT repeat-containing protein 5B
XM_031951273.1	XP_031807133.1	HEAT repeat-containing protein 5B isoform X3

Pathways from BioSystems
Gene Ontology

The following HEATR5B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HEATR5B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa119522	XM_012539862.1 Latest version!	Sarcophilus harrisii HEAT repeat containing 5B (HEATR5B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa150603	XM_023494615.1 Latest version!	Sarcophilus harrisii HEAT repeat containing 5B (HEATR5B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa224489	XM_031951273.1 Latest version!	Sarcophilus harrisii HEAT repeat containing 5B (HEATR5B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa224487	XM_031951270.1 Latest version!	Sarcophilus harrisii HEAT repeat containing 5B (HEATR5B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa224487	XM_031951272.1 Latest version!	Sarcophilus harrisii HEAT repeat containing 5B (HEATR5B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa224488	XM_031951271.1 Latest version!	Sarcophilus harrisii HEAT repeat containing 5B (HEATR5B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HEATR5B ( XM_012539862.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012395316.1 Sarcophilus harrisii HEAT repeat containing 5B (HEATR5B), mRNA.

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CloneID	OSa119522
Clone ID Related Accession (Same CDS sequence)	XM_012539862.1
Accession Version	XM_012539862.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	HEAT repeat-containing protein 5B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816692.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGAGTTGG CCCATAGTTT ATTGCTAAAT GAAGAAGCTT TGGCCCAAAT TACAGAAGCA 
AAGAGGCCTG TTTTTATCTT TGAGTGGTTG CGATTTCTTG ACAAAGTCCT GGTAGCTGCT 
AACAAGACTG ATGTGAAGGA AAAACAGAAA AGGCTTGTTG AACAACTCAC TGGATTAATA 
AGTAGTTCTC CTGGCCCCCC AACAAGGAAA TTACTAGCTA AAAATCTTGC AGCTCTTTAT 
AGCATTGGAG ATACTTTCAC TGTTTTTCAA ACTCTTGACA AGTGTAATGA TATTATCAAA 
AACAAAGATG ATACTGCAGC CTATTTGCCA ACCAAACTGG CTGCAGTAGC ATGTGTTGGG 
GCATTTTATG AAAAAATGGG GAGGATGTTA GGAAGTGCAT TTCCAGAAAC AGTTAACAAT 
CTTTTGAAGT CTCTGAAAAA TGCAGAGTCT CAAGGCCGAA GTGAAATCTT AATGAGTTTA 
CAAAAAGTCC TAAATGGACT GGGTGGTGCA GCAGCTTCTT GTCATCGTGA TATTTATAAG 
AATGCAAGAT CTCTGCTAAG TGACAGATCT ATGGCTGTTA GGTGTGCAGT TGCTAAAGCA 
TATGTTGTTT TTGTTACAAC ATTAGGTGGT CAGTGGTTAG AACGCAGCTT TGCTACATTC 
TTATCCCATG TACTTGATCT GGTTTCACAC CCTCGGGCCA CTCAAACACA TGTAGAAGCT 
GTGTATTCAA GAAGATGTGT CTCCTTTATC CTCAGAGCTA CTGTAGGTAG TTTGCTTGGG 
GAAAAAGCTC AGATTGCAGC TGCCAAAGAA ATCTGCCAAG CCATTGGAAA ACAAATGAAA 
GCAGTGGAAG CAGTTGTCAA TGATACAAGT AGTGAAAACA AATCTGGAGC TGCTGACATA 
GCAGCAAGTC AGCATGTGAT GGTGTGTGCT CTGCAGGAAC TTGGAAGCTT AGTCCAAAGC 
TTAAATGCCA CTGCATCTCC TCTTATTCAG GAAGCATCTA TAGGTCTTTT GGAAACTGTA 
ACATCAGTGC TTCTGCATCC TAGTATGGCT GCCCGACTTG CTGCAGCATG GTGTTTGCGT 
TGTGTAGCTG TGGCCTTGCC TTTCCAATTG ACCCCATTTC TAGACAGATG TGCAGAAAGG 
CTCAATAATT TGAAGACCTC CCCTGAAGCT GTTAGTGGTT ACAGTTTTGC AATGGCTGCT 
TTATTAGGTG GAGTACATCA GTGCCCTTTG GGTATCCCTC ATGCCAAAGG AAAGATGGTA 
GTAAGCATTG CTGAAGATCT CTTACGAACT GCTGCTCAGA ACAGTAGGCT ATCCTTGCAA 
CGTACTCAAG CTGGATGGCT TCTACTTGGA GCACTTATGA CTTTAGGTCC TTCTGTTGTT 
CGTTACCATC TTCCCAAAAT GCTGTTATTA TGGCGAAATG TCTTCCCTCG TTCCTTAAAG 
GAACTAGAAG CTGAAAAGGC ACGAGGAGAT TCTTTTACGT GGCAGGTAAC ATTGGAAGGC 
CGAGCTGGAG CTTTATGTGC CATGAGGAGT TTTGTTGCTC ATTGTCCTGA GCTTTTAACT 
GAGGATGTGA TTAGAAAATT GATGACGCCT ATTGAATGTG CCATGACAAT GATGTCACAC 
ATTCCTTCTG TAATTAAAGC CCATGGAGCT CATTTGAAGG CCAGTGCTGC AATGGTTCGT 
TTAAGACTTT ACGATATTTT GGCATTGTTA CCCCCCAAAA CTTATGAAGG ATCTTTTAAT 
GCACTTCTTA GGGAGCTGGT GGCAGAATTT ACTTTGACAG ATAATTCTGC CAATACAACT 
ACTTCCCTTC TTAGATCCCT CTGCCATTAT GATGACAGTG TTCTTCTTGG TTCCTGGCTT 
CAAGAAACTG ATCATAAATC AATTGAAGAT CAGCTCCAAC CAAATAGTGC ATCTGGAAGT 
GGTGCTTTAG AGCATGACCC ATCCTCCATT TATTTACGAA TACCTGCTGG TGAAGCTGTT 
CCTGGTCCTC TTCCGCTTGG AGTCTCAGTC ATTGATGCTT CTGTTGCTCT CTTTGGTGTC 
GTATTTCCTC ATGTTTCTTA CAAACACCGA TTACAAATGC TGGATCATTT TGCTGAGTGT 
GTCAAACAGG CTAAAGGTGT TCGTCAACAG GCTGTACAGC TTAATATTTT CACTGCTGTT 
CTTAGTGCAT TAAAGGGCTT GGCTGAAAAC AAAAGTACTT TAGGACCTGA GGAAGTTCGG 
AAATCTGCTC TGACATTGGT TATGGGAGCT CTCGATAATC CAAATCCCAT TTTGAGATGT 
GCAGCTGGAG AAGCCCTTGG AAGAATGGCT CAAGTGGTCG GAGAAGCAAC TTTCATTGCT 
AGAATGGCTC AATATAGCTT TGACAAATTA AAATCTGCTC GAGATGTTGT TTCCAGAACT 
GGCCATTCAT TGGCTCTTGG CTGTCTACAT CGATATGTTG GGGGGATAGG ATCAGGACAA 
CACCTCAAGA CCAGTGTTAG TATTCTACTG GCACTAGCTC AAGATGGAAC TTCACCTGAA 
GTCCAGACCT GGTCTCTTCA TTCACTTGCT TTAATAGTGG ACTCTAGTGG GCCAATGTAT 
CGTGGCTATG TTGAACCAAC CTTGTCCCTA GTCCTTACAT TATTATTGAC AGTTCCACCT 
TCACATACAG AGGTTCATCA ATGTTTGGGT CGATGTTTAG GTGCTATAAT AACCACCGTT 
GGACCTGAAC TGCAGGGGAA TGGAGCAACA ATCTCAACAA TCCGCTCTTC CTGTTTGGTT 
GGTTGTGCAA TTACACAAGA CCATTCAGAT TCTCTTGTTC AGGCAGCTGC CATATCTTGC 
CTTCAACAAC TACATATGTT TGCACCACGA CATGTCAATC TATCTAGTCT GGTTCCCAGC 
CTTTGTGTTC ATTTATGTAG TTCCCACTTA TTACTCCGAA GGGCAGCAGT TGCATGTCTT 
CGACAACTTG CACAGCGAGA AGCTGCTGAA GTATGTGAAT ATGCTATGAG CCTTGCAAAA 
AATGCAGGAG ACAAAGAAAG CAGTGGCATC AATATGAATC CTTTTGCACC AGGGGTCAGT 
TCTCGCCGTG ATATCCATTG TCGACACCAA GGTGTCAATA TAACAGAAAC TGGTTTAGAA 
GGGCTTCTTT TTGGAATGCT AGACCGGGAG ACTGATAGAA AATTATGTTC GGATATCCAT 
GATACTTTAG GACATATGCT TTCATCTCTG GCTGTAGACA AACTTTCACA TTGGCTAATG 
CTTTGTAAGG ATGTTCTGGC AGCATCCAGT GATATGAGCA CTACAACCCC TTTAAGTAGT 
GGAAAGGATG AAGAATCTGA GAAGAAGGAT GAGATGGATG ATGATACTAT GTTTACCACA 
TTAGGTGAAG AGGATAAATC AAAGCCATCT GTAGCTCCTC GCTGGGCTAC TCGAGTGTTT 
GCTGCTGACT GCTTGTGTCG AGTCATCATG TTGTGTGAGA ATGCAGATCA GGCTCATTTT 
GATCTTGCTA TGGCACGTTC AGCCAAATTG AAAAATCCTG CAAATGATCT TCTAGTGCTC 
CATCTCTCTG ACCTGATACG CATGGCATTC ATGGCTGCTA CAGATCACAG CAACCAGCTG 
CGGATGGCTG GACTCCAAGC ACTTGAGGAC ATTATCAAGA AATTTGCATC CGTGCCTGAG 
CCAGAATTTC CAGGTCATGT GATTTTGGAG CAGTACCAAG CTAATGTGGG AGCTGCTCTC 
AGACCAGCTT TTTCACAAGA TACTCCATCA GACATAATTG CAAAAGCTTG CCAGGTTTGT 
AGTACATGGA TTGGAAGTGG AGTTGTCAGT GATCTCAATG ATCTCCGTCG AGTACACAAT 
TTGCTTGTTT CCTCACTTGA CAAAGTACAG GCAGGAAAAG GATCTTCCAG CCAACTTTAT 
AGAGAGAGTG CAACAACCAT GGAAAAATTG GCAGTGCTAA AAGCTTGGGC TGAGGTTTAT 
GTTGTTGCCA TGAATATTAA AAAGGAAGCA GAAACTAAAC CAAAAAGAGC CATAAAAAAT 
ACTGATGATG AAGATGATGA TTATGGGACC ATAGATGAGT TGCCACCAGA CAGTTTAATT 
ACACTTGTAC AACCTGAATT GCCAACACTT AGCCGCCTCT GGTTGGCAGC ATTAAAAGAT 
TATGCACTTT TGACTTTACC AGCTGAATTT TCAAGTCAGC TTCCACCAGA TGGGGGAGCA 
TTTTATACTC CAGAGACGAT TGATACGGCT CGACTTCACT ATCGGAACTC TTGGGCTCCA 
ATTCTCCATG CAGTAGCACT TTGGTTAAAT AGCACAGGAT TTACTAATTC AGAAGCAACA 
GAAGAAACAT CAGTATCTGG TTTGCAAAAA CGTCCAACAC CTGTCATTTT AAACCAAATA 
CCTGGAGCAA CAGCTAATGC TAAATCTTTG CCAGAAATCA ACAAGGACAG AATGCATCTG 
ATTTTAGGTG TTAGTATACA GTTTCTCTGT TCCCCAAGAC CTGAGGAACC TATTGAACAT 
GTTACAGCCT GTCTGCAGGC ATTGCATACC TTGCTGGAAT CCCCTTGTGC CAGAATTCAT 
ATTGGAGAAG ATCAGCTCAT TGGTGTTGAG TTGCTGAATG TGCTACATCG ACTCCTTTTG 
ACTTGGAATC CACCCTCAGT TCAGCTGTTG GTTACTGGAG TGGTACAGCA GATAGTTAGA 
GCAGCTCAAG ATTATTTGCA AGAAAAAAGA AACACTCTAA ATGAAGATGA TATGGAAAAA 
GAGAGTTGCC CTGTTCTAGG AGAAGGAGGT GACAATGGTG GTCTTGTGCC TGGAAAATCT 
CTTGTCTTTG CAACCATGGA GCTATTGATG TTCATCTTAG TACGGCACAT GCCACATCTC 
AGTACCAAGA TGTCAGATTC ACCAAGTCAT ATATCCACTA AAACTCGATT ATCTGAAGAA 
AGTGCTCGTT TAGTGGCAGC GGCAGTTACC ATCCTCTCAG ACTTGCCATC TCTTTGCTCT 
CCTGCTGGAT GTATGACAAT ATTACCCACA ATTCTCTTTC TGATTGCAAG AATATTGAAA 
GATACTGCAA TAAAGTCTGG TGATAATCAA GTACCCCCTC CAGTTAATGC AGCTCTACAA 
GGAATTAAAA ACATTGTGAC TCTCTCAATG GCCAAAACTG ATGAAACGCA AAAACAGTGG 
ACTGATCTAA TTCGTAGCAC TCTTGCATGT ATCCTGGAAT ATTCTCAACC AGAAGATTCG 
ATGCCAATTC CCGATGAAGT GAGCATGCTT ACAGCCATTG CACTCTTTCT ATGGTCTGCT 
AGTACTGAAA TAATTGGAGT TCAGTCATTA CAGAATGGTT GCATGAACAG ATTTAAAAAT 
GCACTGAATT CTTGTGATCC TTGGCAATAT GCATGCACTA TAAAAGCTCA AAATTTGGTC 
AAATTGTCTT ATGATGTAAT TTATCCTTTA ATACTTTATT TTTCAGGAGT CCAGCTACTT 
GCTCTTTTAG TTCCAACTTT GATTTCATAT TTGTTAGATG AAAATGCTTT TGATTCAGCA 
CTTACGGCAT CCAAAGATCT TCATGAATTT GCACTCCAGA ATTTAATGCA CATTGGGCCT 
CTCTATCCAC ATGCTTTCAA GACTGTTATG GGAGCTGCTC CTGAATTGAA AACAAGACTC 
GAAACTGCAG TTCGCGCAAG TCAAGCTAGT AAAGCAAAAG CAGCTGCCAG GCAACCAGCA 
CCTACCATAC ATTCCGCTCC AACAATTAAA CTCAAAACAA GCTTTTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012395316.1
CDS	78..5828
Translation	MELAHSLLLNEEALAQITEAKRPVFIFEWLRFLDKVLVAANKTDVKEKQKRLVEQLTGLISSSPGPPTRKLLAKNLAALYSIGDTFTVFQTLDKCNDIIKNKDDTAAYLPTKLAAVACVGAFYEKMGRMLGSAFPETVNNLLKSLKNAESQGRSEILMSLQKVLNGLGGAAASCHRDIYKNARSLLSDRSMAVRCAVAKAYVVFVTTLGGQWLERSFATFLSHVLDLVSHPRATQTHVEAVYSRRCVSFILRATVGSLLGEKAQIAAAKEICQAIGKQMKAVEAVVNDTSSENKSGAADIAASQHVMVCALQELGSLVQSLNATASPLIQEASIGLLETVTSVLLHPSMAARLAAAWCLRCVAVALPFQLTPFLDRCAERLNNLKTSPEAVSGYSFAMAALLGGVHQCPLGIPHAKGKMVVSIAEDLLRTAAQNSRLSLQRTQAGWLLLGALMTLGPSVVRYHLPKMLLLWRNVFPRSLKELEAEKARGDSFTWQVTLEGRAGALCAMRSFVAHCPELLTEDVIRKLMTPIECAMTMMSHIPSVIKAHGAHLKASAAMVRLRLYDILALLPPKTYEGSFNALLRELVAEFTLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAVPGPLPLGVSVIDASVALFGVVFPHVSYKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAENKSTLGPEEVRKSALTLVMGALDNPNPILRCAAGEALGRMAQVVGEATFIARMAQYSFDKLKSARDVVSRTGHSLALGCLHRYVGGIGSGQHLKTSVSILLALAQDGTSPEVQTWSLHSLALIVDSSGPMYRGYVEPTLSLVLTLLLTVPPSHTEVHQCLGRCLGAIITTVGPELQGNGATISTIRSSCLVGCAITQDHSDSLVQAAAISCLQQLHMFAPRHVNLSSLVPSLCVHLCSSHLLLRRAAVACLRQLAQREAAEVCEYAMSLAKNAGDKESSGINMNPFAPGVSSRRDIHCRHQGVNITETGLEGLLFGMLDRETDRKLCSDIHDTLGHMLSSLAVDKLSHWLMLCKDVLAASSDMSTTTPLSSGKDEESEKKDEMDDDTMFTTLGEEDKSKPSVAPRWATRVFAADCLCRVIMLCENADQAHFDLAMARSAKLKNPANDLLVLHLSDLIRMAFMAATDHSNQLRMAGLQALEDIIKKFASVPEPEFPGHVILEQYQANVGAALRPAFSQDTPSDIIAKACQVCSTWIGSGVVSDLNDLRRVHNLLVSSLDKVQAGKGSSSQLYRESATTMEKLAVLKAWAEVYVVAMNIKKEAETKPKRAIKNTDDEDDDYGTIDELPPDSLITLVQPELPTLSRLWLAALKDYALLTLPAEFSSQLPPDGGAFYTPETIDTARLHYRNSWAPILHAVALWLNSTGFTNSEATEETSVSGLQKRPTPVILNQIPGATANAKSLPEINKDRMHLILGVSIQFLCSPRPEEPIEHVTACLQALHTLLESPCARIHIGEDQLIGVELLNVLHRLLLTWNPPSVQLLVTGVVQQIVRAAQDYLQEKRNTLNEDDMEKESCPVLGEGGDNGGLVPGKSLVFATMELLMFILVRHMPHLSTKMSDSPSHISTKTRLSEESARLVAAAVTILSDLPSLCSPAGCMTILPTILFLIARILKDTAIKSGDNQVPPPVNAALQGIKNIVTLSMAKTDETQKQWTDLIRSTLACILEYSQPEDSMPIPDEVSMLTAIALFLWSASTEIIGVQSLQNGCMNRFKNALNSCDPWQYACTIKAQNLVKLSYDVIYPLILYFSGVQLLALLVPTLISYLLDENAFDSALTASKDLHEFALQNLMHIGPLYPHAFKTVMGAAPELKTRLETAVRASQASKAKAAARQPAPTIHSAPTIKLKTSFF

Target ORF information:

RefSeq Version	XM_012539862.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HEAT repeat containing 5B (HEATR5B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012539862.1

ORF Insert Sequence:

ATGGAGTTGG CCCATAGTTT ATTGCTAAAT GAAGAAGCTT TGGCCCAAAT TACAGAAGCA 
AAGAGGCCTG TTTTTATCTT TGAGTGGTTG CGATTTCTTG ACAAAGTCCT GGTAGCTGCT 
AACAAGACTG ATGTGAAGGA AAAACAGAAA AGGCTTGTTG AACAACTCAC TGGATTAATA 
AGTAGTTCTC CTGGCCCCCC AACAAGGAAA TTACTAGCTA AAAATCTTGC AGCTCTTTAT 
AGCATTGGAG ATACTTTCAC TGTTTTTCAA ACTCTTGACA AGTGTAATGA TATTATCAAA 
AACAAAGATG ATACTGCAGC CTATTTGCCA ACCAAACTGG CTGCAGTAGC ATGTGTTGGG 
GCATTTTATG AAAAAATGGG GAGGATGTTA GGAAGTGCAT TTCCAGAAAC AGTTAACAAT 
CTTTTGAAGT CTCTGAAAAA TGCAGAGTCT CAAGGCCGAA GTGAAATCTT AATGAGTTTA 
CAAAAAGTCC TAAATGGACT GGGTGGTGCA GCAGCTTCTT GTCATCGTGA TATTTATAAG 
AATGCAAGAT CTCTGCTAAG TGACAGATCT ATGGCTGTTA GGTGTGCAGT TGCTAAAGCA 
TATGTTGTTT TTGTTACAAC ATTAGGTGGT CAGTGGTTAG AACGCAGCTT TGCTACATTC 
TTATCCCATG TACTTGATCT GGTTTCACAC CCTCGGGCCA CTCAAACACA TGTAGAAGCT 
GTGTATTCAA GAAGATGTGT CTCCTTTATC CTCAGAGCTA CTGTAGGTAG TTTGCTTGGG 
GAAAAAGCTC AGATTGCAGC TGCCAAAGAA ATCTGCCAAG CCATTGGAAA ACAAATGAAA 
GCAGTGGAAG CAGTTGTCAA TGATACAAGT AGTGAAAACA AATCTGGAGC TGCTGACATA 
GCAGCAAGTC AGCATGTGAT GGTGTGTGCT CTGCAGGAAC TTGGAAGCTT AGTCCAAAGC 
TTAAATGCCA CTGCATCTCC TCTTATTCAG GAAGCATCTA TAGGTCTTTT GGAAACTGTA 
ACATCAGTGC TTCTGCATCC TAGTATGGCT GCCCGACTTG CTGCAGCATG GTGTTTGCGT 
TGTGTAGCTG TGGCCTTGCC TTTCCAATTG ACCCCATTTC TAGACAGATG TGCAGAAAGG 
CTCAATAATT TGAAGACCTC CCCTGAAGCT GTTAGTGGTT ACAGTTTTGC AATGGCTGCT 
TTATTAGGTG GAGTACATCA GTGCCCTTTG GGTATCCCTC ATGCCAAAGG AAAGATGGTA 
GTAAGCATTG CTGAAGATCT CTTACGAACT GCTGCTCAGA ACAGTAGGCT ATCCTTGCAA 
CGTACTCAAG CTGGATGGCT TCTACTTGGA GCACTTATGA CTTTAGGTCC TTCTGTTGTT 
CGTTACCATC TTCCCAAAAT GCTGTTATTA TGGCGAAATG TCTTCCCTCG TTCCTTAAAG 
GAACTAGAAG CTGAAAAGGC ACGAGGAGAT TCTTTTACGT GGCAGGTAAC ATTGGAAGGC 
CGAGCTGGAG CTTTATGTGC CATGAGGAGT TTTGTTGCTC ATTGTCCTGA GCTTTTAACT 
GAGGATGTGA TTAGAAAATT GATGACGCCT ATTGAATGTG CCATGACAAT GATGTCACAC 
ATTCCTTCTG TAATTAAAGC CCATGGAGCT CATTTGAAGG CCAGTGCTGC AATGGTTCGT 
TTAAGACTTT ACGATATTTT GGCATTGTTA CCCCCCAAAA CTTATGAAGG ATCTTTTAAT 
GCACTTCTTA GGGAGCTGGT GGCAGAATTT ACTTTGACAG ATAATTCTGC CAATACAACT 
ACTTCCCTTC TTAGATCCCT CTGCCATTAT GATGACAGTG TTCTTCTTGG TTCCTGGCTT 
CAAGAAACTG ATCATAAATC AATTGAAGAT CAGCTCCAAC CAAATAGTGC ATCTGGAAGT 
GGTGCTTTAG AGCATGACCC ATCCTCCATT TATTTACGAA TACCTGCTGG TGAAGCTGTT 
CCTGGTCCTC TTCCGCTTGG AGTCTCAGTC ATTGATGCTT CTGTTGCTCT CTTTGGTGTC 
GTATTTCCTC ATGTTTCTTA CAAACACCGA TTACAAATGC TGGATCATTT TGCTGAGTGT 
GTCAAACAGG CTAAAGGTGT TCGTCAACAG GCTGTACAGC TTAATATTTT CACTGCTGTT 
CTTAGTGCAT TAAAGGGCTT GGCTGAAAAC AAAAGTACTT TAGGACCTGA GGAAGTTCGG 
AAATCTGCTC TGACATTGGT TATGGGAGCT CTCGATAATC CAAATCCCAT TTTGAGATGT 
GCAGCTGGAG AAGCCCTTGG AAGAATGGCT CAAGTGGTCG GAGAAGCAAC TTTCATTGCT 
AGAATGGCTC AATATAGCTT TGACAAATTA AAATCTGCTC GAGATGTTGT TTCCAGAACT 
GGCCATTCAT TGGCTCTTGG CTGTCTACAT CGATATGTTG GGGGGATAGG ATCAGGACAA 
CACCTCAAGA CCAGTGTTAG TATTCTACTG GCACTAGCTC AAGATGGAAC TTCACCTGAA 
GTCCAGACCT GGTCTCTTCA TTCACTTGCT TTAATAGTGG ACTCTAGTGG GCCAATGTAT 
CGTGGCTATG TTGAACCAAC CTTGTCCCTA GTCCTTACAT TATTATTGAC AGTTCCACCT 
TCACATACAG AGGTTCATCA ATGTTTGGGT CGATGTTTAG GTGCTATAAT AACCACCGTT 
GGACCTGAAC TGCAGGGGAA TGGAGCAACA ATCTCAACAA TCCGCTCTTC CTGTTTGGTT 
GGTTGTGCAA TTACACAAGA CCATTCAGAT TCTCTTGTTC AGGCAGCTGC CATATCTTGC 
CTTCAACAAC TACATATGTT TGCACCACGA CATGTCAATC TATCTAGTCT GGTTCCCAGC 
CTTTGTGTTC ATTTATGTAG TTCCCACTTA TTACTCCGAA GGGCAGCAGT TGCATGTCTT 
CGACAACTTG CACAGCGAGA AGCTGCTGAA GTATGTGAAT ATGCTATGAG CCTTGCAAAA 
AATGCAGGAG ACAAAGAAAG CAGTGGCATC AATATGAATC CTTTTGCACC AGGGGTCAGT 
TCTCGCCGTG ATATCCATTG TCGACACCAA GGTGTCAATA TAACAGAAAC TGGTTTAGAA 
GGGCTTCTTT TTGGAATGCT AGACCGGGAG ACTGATAGAA AATTATGTTC GGATATCCAT 
GATACTTTAG GACATATGCT TTCATCTCTG GCTGTAGACA AACTTTCACA TTGGCTAATG 
CTTTGTAAGG ATGTTCTGGC AGCATCCAGT GATATGAGCA CTACAACCCC TTTAAGTAGT 
GGAAAGGATG AAGAATCTGA GAAGAAGGAT GAGATGGATG ATGATACTAT GTTTACCACA 
TTAGGTGAAG AGGATAAATC AAAGCCATCT GTAGCTCCTC GCTGGGCTAC TCGAGTGTTT 
GCTGCTGACT GCTTGTGTCG AGTCATCATG TTGTGTGAGA ATGCAGATCA GGCTCATTTT 
GATCTTGCTA TGGCACGTTC AGCCAAATTG AAAAATCCTG CAAATGATCT TCTAGTGCTC 
CATCTCTCTG ACCTGATACG CATGGCATTC ATGGCTGCTA CAGATCACAG CAACCAGCTG 
CGGATGGCTG GACTCCAAGC ACTTGAGGAC ATTATCAAGA AATTTGCATC CGTGCCTGAG 
CCAGAATTTC CAGGTCATGT GATTTTGGAG CAGTACCAAG CTAATGTGGG AGCTGCTCTC 
AGACCAGCTT TTTCACAAGA TACTCCATCA GACATAATTG CAAAAGCTTG CCAGGTTTGT 
AGTACATGGA TTGGAAGTGG AGTTGTCAGT GATCTCAATG ATCTCCGTCG AGTACACAAT 
TTGCTTGTTT CCTCACTTGA CAAAGTACAG GCAGGAAAAG GATCTTCCAG CCAACTTTAT 
AGAGAGAGTG CAACAACCAT GGAAAAATTG GCAGTGCTAA AAGCTTGGGC TGAGGTTTAT 
GTTGTTGCCA TGAATATTAA AAAGGAAGCA GAAACTAAAC CAAAAAGAGC CATAAAAAAT 
ACTGATGATG AAGATGATGA TTATGGGACC ATAGATGAGT TGCCACCAGA CAGTTTAATT 
ACACTTGTAC AACCTGAATT GCCAACACTT AGCCGCCTCT GGTTGGCAGC ATTAAAAGAT 
TATGCACTTT TGACTTTACC AGCTGAATTT TCAAGTCAGC TTCCACCAGA TGGGGGAGCA 
TTTTATACTC CAGAGACGAT TGATACGGCT CGACTTCACT ATCGGAACTC TTGGGCTCCA 
ATTCTCCATG CAGTAGCACT TTGGTTAAAT AGCACAGGAT TTACTAATTC AGAAGCAACA 
GAAGAAACAT CAGTATCTGG TTTGCAAAAA CGTCCAACAC CTGTCATTTT AAACCAAATA 
CCTGGAGCAA CAGCTAATGC TAAATCTTTG CCAGAAATCA ACAAGGACAG AATGCATCTG 
ATTTTAGGTG TTAGTATACA GTTTCTCTGT TCCCCAAGAC CTGAGGAACC TATTGAACAT 
GTTACAGCCT GTCTGCAGGC ATTGCATACC TTGCTGGAAT CCCCTTGTGC CAGAATTCAT 
ATTGGAGAAG ATCAGCTCAT TGGTGTTGAG TTGCTGAATG TGCTACATCG ACTCCTTTTG 
ACTTGGAATC CACCCTCAGT TCAGCTGTTG GTTACTGGAG TGGTACAGCA GATAGTTAGA 
GCAGCTCAAG ATTATTTGCA AGAAAAAAGA AACACTCTAA ATGAAGATGA TATGGAAAAA 
GAGAGTTGCC CTGTTCTAGG AGAAGGAGGT GACAATGGTG GTCTTGTGCC TGGAAAATCT 
CTTGTCTTTG CAACCATGGA GCTATTGATG TTCATCTTAG TACGGCACAT GCCACATCTC 
AGTACCAAGA TGTCAGATTC ACCAAGTCAT ATATCCACTA AAACTCGATT ATCTGAAGAA 
AGTGCTCGTT TAGTGGCAGC GGCAGTTACC ATCCTCTCAG ACTTGCCATC TCTTTGCTCT 
CCTGCTGGAT GTATGACAAT ATTACCCACA ATTCTCTTTC TGATTGCAAG AATATTGAAA 
GATACTGCAA TAAAGTCTGG TGATAATCAA GTACCCCCTC CAGTTAATGC AGCTCTACAA 
GGAATTAAAA ACATTGTGAC TCTCTCAATG GCCAAAACTG ATGAAACGCA AAAACAGTGG 
ACTGATCTAA TTCGTAGCAC TCTTGCATGT ATCCTGGAAT ATTCTCAACC AGAAGATTCG 
ATGCCAATTC CCGATGAAGT GAGCATGCTT ACAGCCATTG CACTCTTTCT ATGGTCTGCT 
AGTACTGAAA TAATTGGAGT TCAGTCATTA CAGAATGGTT GCATGAACAG ATTTAAAAAT 
GCACTGAATT CTTGTGATCC TTGGCAATAT GCATGCACTA TAAAAGCTCA AAATTTGGTC 
AAATTGTCTT ATGATGTAAT TTATCCTTTA ATACTTTATT TTTCAGGAGT CCAGCTACTT 
GCTCTTTTAG TTCCAACTTT GATTTCATAT TTGTTAGATG AAAATGCTTT TGATTCAGCA 
CTTACGGCAT CCAAAGATCT TCATGAATTT GCACTCCAGA ATTTAATGCA CATTGGGCCT 
CTCTATCCAC ATGCTTTCAA GACTGTTATG GGAGCTGCTC CTGAATTGAA AACAAGACTC 
GAAACTGCAG TTCGCGCAAG TCAAGCTAGT AAAGCAAAAG CAGCTGCCAG GCAACCAGCA 
CCTACCATAC ATTCCGCTCC AACAATTAAA CTCAAAACAA GCTTTTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5B ( XM_023494615.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023350383.1 Sarcophilus harrisii HEAT repeat containing 5B (HEATR5B), mRNA.

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CloneID	OSa150603
Clone ID Related Accession (Same CDS sequence)	XM_023494615.1
Accession Version	XM_023494615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	HEAT repeat-containing protein 5B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816692.1) and transcript sequence (GEDN01055208.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases assembly gap :: added 214 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGAGTTGG CCCATAGTTT ATTGCTAAAT GAAGAAGCTT TGGCCCAAAT TACAGAAGCA 
AAGAGGCCTG TTTTTATCTT TGAGTGGTTG CGATTTCTTG ACAAAGTCCT GGTAGCTGCT 
AACAAGACTG ATGTGAAGGA AAAACAGAAA AGGCTTGTTG AACAACTCAC TGGATTAATA 
AGTAGTTCTC CTGGCCCCCC AACAAGGAAA TTACTAGCTA AAAATCTTGC AGCTCTTTAT 
AGCATTGGAG ATACTTTCAC TGTTTTTCAA ACTCTTGACA AGTGTAATGA TATTATCAAA 
AACAAAGATG ATACTGCAGC CTATTTGCCA ACCAAACTGG CTGCAGTAGC ATGTGTTGGG 
GCATTTTATG AAAAAATGGG GAGGATGTTA GGAAGTGCAT TTCCAGAAAC AGTTAACAAT 
CTTTTGAAGT CTCTGAAAAA TGCAGAGTCT CAAGGCCGAA GTGAAATCTT AATGAGTTTA 
CAAAAAGTCC TAAATGGACT GGGTGGTGCA GCAGCTTCTT GTCATCGTGA TATTTATAAG 
AATGCAAGAT CTCTGCTAAG TGACAGATCT ATGGCTGTTA GGTGTGCAGT TGCTAAATAC 
AGTTTGCAAC TTCTTAATGC CTCATTATTC ATATCCTCCC ATGAGTTATC CATCAAGAAT 
TTCTGCTTGC AGGCATATGT TGTTTTTGTT ACAACATTAG GTGGTCAGTG GTTAGAACGC 
AGCTTTGCTA CATTCTTATC CCATGTACTT GATCTGGTTT CACACCCTCG GGCCACTCAA 
ACACATGTAG AAGCTGTGTA TTCAAGAAGA TGTGTCTCCT TTATCCTCAG AGCTACTGTA 
GGTAGTTTGC TTGGGGAAAA AGCTCAGATT GCAGCTGCCA AAGAAATCTG CCAAGCCATT 
GGAAAACAAA TGAAAGCAGT GGAAGCAGTT GTCAATGATA CAAGTAGTGA AAACAAATCT 
GGAGCTGCTG ACATAGCAGC AAGTCAGCAT GTGATGGTGT GTGCTCTGCA GGAACTTGGA 
AGCTTAGTCC AAAGCTTAAA TGCCACTGCA TCTCCTCTTA TTCAGGAAGC ATCTATAGGT 
CTTTTGGAAA CTGTAACATC AGTGCTTCTG CATCCTAGTA TGGCTGCCCG ACTTGCTGCA 
GCATGGTGTT TGCGTTGTGT AGCTGTGGCC TTGCCTTTCC AATTGACCCC ATTTCTAGAC 
AGATGTGCAG AAAGGCTCAA TAATTTGAAG ACCTCCCCTG AAGCTGTTAG TGGTTACAGT 
TTTGCAATGG CTGCTTTATT AGGTGGAGTA CATCAGTGCC CTTTGGGTAT CCCTCATGCC 
AAAGGAAAGA TGGTAGTAAG CATTGCTGAA GATCTCTTAC GAACTGCTGC TCAGAACAGT 
AGGCTATCCT TGCAACGTAC TCAAGCTGGA TGGCTTCTAC TTGGAGCACT TATGACTTTA 
GGTCCTTCTG TTGTTCGTTA CCATCTTCCC AAAATGCTGT TATTATGGCG AAATGTCTTC 
CCTCGTTCCT TAAAGGAACT AGAAGCTGAA AAGGCACGAG GAGATTCTTT TACGTGGCAG 
GTAACATTGG AAGGCCGAGC TGGAGCTTTA TGTGCCATGA GGAGTTTTGT TGCTCATTGT 
CCTGAGCTTT TAACTGAGGA TGTGATTAGA AAATTGATGA CGCCTATTGA ATGTGCCATG 
ACAATGATGT CACACATTCC TTCTGTAATT AAAGCCCATG GAGCTCATTT GAAGGCCAGT 
GCTGCAATGG TTCGTTTAAG ACTTTACGAT ATTTTGGCAT TGTTACCCCC CAAAACTTAT 
GAAGGATCTT TTAATGCACT TCTTAGGGAG CTGGTGGCAG AATTTACTTT GACAGATAAT 
TCTGCCAATA CAACTACTTC CCTTCTTAGA TCCCTCTGCC ATTATGATGA CAGTGTTCTT 
CTTGGTTCCT GGCTTCAAGA AACTGATCAT AAATCAATTG AAGATCAGCT CCAACCAAAT 
AGTGCATCTG GAAGTGGTGC TTTAGAGCAT GACCCATCCT CCATTTATTT ACGAATACCT 
GCTGGTGAAG CTGTTCCTGG TCCTCTTCCG CTTGGAGTCT CAGTCATTGA TGCTTCTGTT 
GCTCTCTTTG GTGTCGTATT TCCTCATGTT TCTTACAAAC ACCGATTACA AATGCTGGAT 
CATTTTGCTG AGTGTGTCAA ACAGGCTAAA GGTGTTCGTC AACAGGCTGT ACAGCTTAAT 
ATTTTCACTG CTGTTCTTAG TGCATTAAAG GGCTTGGCTG AAAACAAAAG TACTTTAGGA 
CCTGAGGAAG TTCGGAAATC TGCTCTGACA TTGGTTATGG GAGCTCTCGA TAATCCAAAT 
CCCATTTTGA GATGTGCAGC TGGAGAAGCC CTTGGAAGAA TGGCTCAAGT GGTCGGAGAA 
GCAACTTTCA TTGCTAGAAT GGCTCAATAT AGCTTTGACA AATTAAAATC TGCTCGAGAT 
GTTGTTTCCA GAACTGGCCA TTCATTGGCT CTTGGCTGTC TACATCGATA TGTTGGGGGG 
ATAGGATCAG GACAACACCT CAAGACCAGT GTTAGTATTC TACTGGCACT AGCTCAAGAT 
GGAACTTCAC CTGAAGTCCA GACCTGGTCT CTTCATTCAC TTGCTTTAAT AGTGGACTCT 
AGTGGGCCAA TGTATCGTGG CTATGTTGAA CCAACCTTGT CCCTAGTCCT TACATTATTA 
TTGACAGTTC CACCTTCACA TACAGAGGTT CATCAATGTT TGGGTCGATG TTTAGGTGCT 
ATAATAACCA CCGTTGGACC TGAACTGCAG GGGAATGGAG CAACAATCTC AACAATCCGC 
TCTTCCTGTT TGGTTGGTTG TGCAATTACA CAAGACCATT CAGATTCTCT TGTTCAGGCA 
GCTGCCATAT CTTGCCTTCA ACAACTACAT ATGTTTGCAC CACGACATGT CAATCTATCT 
AGTCTGGTTC CCAGCCTTTG TGTTCATTTA TGTAGTTCCC ACTTATTACT CCGAAGGGCA 
GCAGTTGCAT GTCTTCGACA ACTTGCACAG CGAGAAGCTG CTGAAGTATG TGAATATGCT 
ATGAGCCTTG CAAAAAATGC AGGAGACAAA GAAAGCAGTG GCATCAATAT GAATCCTTTT 
GCACCAGGGG TCAGTTCTCG CCGTGATATC CATTGTCGAC ACCAAGGTGT CAATATAACA 
GAAACTGGTT TAGAAGGGCT TCTTTTTGGA ATGCTAGACC GGGAGACTGA TAGAAAATTA 
TGTTCGGATA TCCATGATAC TTTAGGACAT ATGCTTTCAT CTCTGGCTGT AGACAAACTT 
TCACATTGGC TAATGCTTTG TAAGGATGTT CTGGCAGCAT CCAGTGATAT GAGCACTACA 
ACCCCTTTAA GTAGTGGAAA GGATGAAGAA TCTGAGAAGA AGGATGAGAT GGATGATGAT 
ACTATGTTTA CCACATTAGG TGAAGAGGAT AAATCAAAGC CATCTGTAGC TCCTCGCTGG 
GCTACTCGAG TGTTTGCTGC TGACTGCTTG TGTCGAGTCA TCATGTTGTG TGAGAATGCA 
GATCAGGCTC ATTTTGATCT TGCTATGGCA CGTTCAGCCA AATTGAAAAA TCCTGCAAAT 
GATCTTCTAG TGCTCCATCT CTCTGACCTG ATACGCATGG CATTCATGGC TGCTACAGAT 
CACAGCAACC AGCTGCGGAT GGCTGGACTC CAAGCACTTG AGGACATTAT CAAGAAATTT 
GCATCCGTGC CTGAGCCAGA ATTTCCAGGT CATGTGATTT TGGAGCAGTA CCAAGCTAAT 
GTGGGAGCTG CTCTCAGACC AGCTTTTTCA CAAGATACTC CATCAGACAT AATTGCAAAA 
GCTTGCCAGG TTTGTAGTAC ATGGATTGGA AGTGGAGTTG TCAGTGATCT CAATGATCTC 
CGTCGAGTAC ACAATTTGCT TGTTTCCTCA CTTGACAAAG TACAGGCAGG AAAAGGATCT 
TCCAGCCAAC TTTATAGAGA GAGTGCAACA ACCATGGAAA AATTGGCAGT GCTAAAAGCT 
TGGGCTGAGG TTTATGTTGT TGCCATGAAT ATTAAAAAGG AAGCAGAAAC TAAACCAAAA 
AGAGCCATAA AAAATACTGA TGATGAAGAT GATGATTATG GGACCATAGA TGAGTTGCCA 
CCAGACAGTT TAATTACACT TGTACAACCT GAATTGCCAA CACTTAGCCG CCTCTGGTTG 
GCAGCATTAA AAGATTATGC ACTTTTGACT TTACCAGCTG AATTTTCAAG TCAGCTTCCA 
CCAGATGGGG GAGCATTTTA TACTCCAGAG ACGATTGATA CGGCTCGACT TCACTATCGG 
AACTCTTGGG CTCCAATTCT CCATGCAGTA GCACTTTGGT TAAATAGCAC AGGATTTACT 
AATTCAGAAG CAACAGAAGA AACATCAGTA TCTGGTTTGC AAAAACGTCC AACACCTGTC 
ATTTTAAACC AAATACCTGG AGCAACAGCT AATGCTAAAT CTTTGCCAGA AATCAACAAG 
GACAGAATGC ATCTGATTTT AGGTGTTAGT ATACAGTTTC TCTGTTCCCC AAGACCTGAG 
GAACCTATTG AACATGTTAC AGCCTGTCTG CAGGCATTGC ATACCTTGCT GGAATCCCCT 
TGTGCCAGAA TTCATATTGG AGAAGATCAG CTCATTGGTG TTGAGTTGCT GAATGTGCTA 
CATCGACTCC TTTTGACTTG GAATCCACCC TCAGTTCAGC TGTTGGTTAC TGGAGTGGTA 
CAGCAGATAG TTAGAGCAGC TCAAGATTAT TTGCAAGAAA AAAGAAACAC TCTAAATGAA 
GATGATATGG AAAAAGAGAG TTGCCCTGTT CTAGGAGAAG GAGGTGACAA TGGTGGTCTT 
GTGCCTGGAA AATCTCTTGT CTTTGCAACC ATGGAGCTAT TGATGTTCAT CTTAGTACGG 
CACATGCCAC ATCTCAGTAC CAAGATGTCA GATTCACCAA GTCATATATC CACTAAAACT 
CGATTATCTG AAGAAAGTGC TCGTTTAGTG GCAGCGGCAG TTACCATCCT CTCAGACTTG 
CCATCTCTTT GCTCTCCTGC TGGATGTATG ACAATATTAC CCACAATTCT CTTTCTGATT 
GCAAGAATAT TGAAAGATAC TGCAATAAAG TCTGGTGATA ATCAAGTACC CCCTCCAGTT 
AATGCAGCTC TACAAGGAAT TAAAAACATT GTGACTCTCT CAATGGCCAA AACTGATGAA 
ACGCAAAAAC AGTGGACTGA TCTAATTCGT AGCACTCTTG CATGTATCCT GGAATATTCT 
CAACCAGAAG ATTCGATGCC AATTCCCGAT GAAGTGAGCA TGCTTACAGC CATTGCACTC 
TTTCTATGGT CTGCTAGTAC TGAAATAATT GGAGTTCAGT CATTACAGAA TGGTTGCATG 
AACAGATTTA AAAATGCACT GAATTCTTGT GATCCTTGGG TTCAAGCTAA GTGTTACCAG 
CTTCTTCTTT CAGTCTTCCA GCATTCCAAC CGTGCCCTTT CAACTCCCTA CATCCATTCA 
TTAGCTCCAA TAATGGTTGA AAAACTAAAA GCTGTTGAAA GGAGCCGGCC AACCAGTAGT 
ACAGAGCTTT TAGCTGTTCA AGAAGGAATC AAAGTTCTTG AAACATTGGT TGCCCTTGGT 
GAAGAGCAGA ACAGAGTCCA GCTACTTGCT CTTTTAGTTC CAACTTTGAT TTCATATTTG 
TTAGATGAAA ATGCTTTTGA TTCAGCACTT ACGGCATCCA AAGATCTTCA TGAATTTGCA 
CTCCAGAATT TAATGCACAT TGGGCCTCTC TATCCACATG CTTTCAAGAC TGTTATGGGA 
GCTGCTCCTG AATTGAAAAC AAGACTCGAA ACTGCAGTTC GCGCAAGTCA AGCTAGTAAA 
GCAAAAGCAG CTGCCAGGCA ACCAGCACCT ACCATACATT CCGCTCCAAC AATTAAACTC 
AAAACAAGCT TTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023350383.1
CDS	817..6774
Translation	MELAHSLLLNEEALAQITEAKRPVFIFEWLRFLDKVLVAANKTDVKEKQKRLVEQLTGLISSSPGPPTRKLLAKNLAALYSIGDTFTVFQTLDKCNDIIKNKDDTAAYLPTKLAAVACVGAFYEKMGRMLGSAFPETVNNLLKSLKNAESQGRSEILMSLQKVLNGLGGAAASCHRDIYKNARSLLSDRSMAVRCAVAKYSLQLLNASLFISSHELSIKNFCLQAYVVFVTTLGGQWLERSFATFLSHVLDLVSHPRATQTHVEAVYSRRCVSFILRATVGSLLGEKAQIAAAKEICQAIGKQMKAVEAVVNDTSSENKSGAADIAASQHVMVCALQELGSLVQSLNATASPLIQEASIGLLETVTSVLLHPSMAARLAAAWCLRCVAVALPFQLTPFLDRCAERLNNLKTSPEAVSGYSFAMAALLGGVHQCPLGIPHAKGKMVVSIAEDLLRTAAQNSRLSLQRTQAGWLLLGALMTLGPSVVRYHLPKMLLLWRNVFPRSLKELEAEKARGDSFTWQVTLEGRAGALCAMRSFVAHCPELLTEDVIRKLMTPIECAMTMMSHIPSVIKAHGAHLKASAAMVRLRLYDILALLPPKTYEGSFNALLRELVAEFTLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAVPGPLPLGVSVIDASVALFGVVFPHVSYKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAENKSTLGPEEVRKSALTLVMGALDNPNPILRCAAGEALGRMAQVVGEATFIARMAQYSFDKLKSARDVVSRTGHSLALGCLHRYVGGIGSGQHLKTSVSILLALAQDGTSPEVQTWSLHSLALIVDSSGPMYRGYVEPTLSLVLTLLLTVPPSHTEVHQCLGRCLGAIITTVGPELQGNGATISTIRSSCLVGCAITQDHSDSLVQAAAISCLQQLHMFAPRHVNLSSLVPSLCVHLCSSHLLLRRAAVACLRQLAQREAAEVCEYAMSLAKNAGDKESSGINMNPFAPGVSSRRDIHCRHQGVNITETGLEGLLFGMLDRETDRKLCSDIHDTLGHMLSSLAVDKLSHWLMLCKDVLAASSDMSTTTPLSSGKDEESEKKDEMDDDTMFTTLGEEDKSKPSVAPRWATRVFAADCLCRVIMLCENADQAHFDLAMARSAKLKNPANDLLVLHLSDLIRMAFMAATDHSNQLRMAGLQALEDIIKKFASVPEPEFPGHVILEQYQANVGAALRPAFSQDTPSDIIAKACQVCSTWIGSGVVSDLNDLRRVHNLLVSSLDKVQAGKGSSSQLYRESATTMEKLAVLKAWAEVYVVAMNIKKEAETKPKRAIKNTDDEDDDYGTIDELPPDSLITLVQPELPTLSRLWLAALKDYALLTLPAEFSSQLPPDGGAFYTPETIDTARLHYRNSWAPILHAVALWLNSTGFTNSEATEETSVSGLQKRPTPVILNQIPGATANAKSLPEINKDRMHLILGVSIQFLCSPRPEEPIEHVTACLQALHTLLESPCARIHIGEDQLIGVELLNVLHRLLLTWNPPSVQLLVTGVVQQIVRAAQDYLQEKRNTLNEDDMEKESCPVLGEGGDNGGLVPGKSLVFATMELLMFILVRHMPHLSTKMSDSPSHISTKTRLSEESARLVAAAVTILSDLPSLCSPAGCMTILPTILFLIARILKDTAIKSGDNQVPPPVNAALQGIKNIVTLSMAKTDETQKQWTDLIRSTLACILEYSQPEDSMPIPDEVSMLTAIALFLWSASTEIIGVQSLQNGCMNRFKNALNSCDPWVQAKCYQLLLSVFQHSNRALSTPYIHSLAPIMVEKLKAVERSRPTSSTELLAVQEGIKVLETLVALGEEQNRVQLLALLVPTLISYLLDENAFDSALTASKDLHEFALQNLMHIGPLYPHAFKTVMGAAPELKTRLETAVRASQASKAKAAARQPAPTIHSAPTIKLKTSFF

Target ORF information:

RefSeq Version	XM_023494615.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HEAT repeat containing 5B (HEATR5B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023494615.1

ORF Insert Sequence:

ATGGAGTTGG CCCATAGTTT ATTGCTAAAT GAAGAAGCTT TGGCCCAAAT TACAGAAGCA 
AAGAGGCCTG TTTTTATCTT TGAGTGGTTG CGATTTCTTG ACAAAGTCCT GGTAGCTGCT 
AACAAGACTG ATGTGAAGGA AAAACAGAAA AGGCTTGTTG AACAACTCAC TGGATTAATA 
AGTAGTTCTC CTGGCCCCCC AACAAGGAAA TTACTAGCTA AAAATCTTGC AGCTCTTTAT 
AGCATTGGAG ATACTTTCAC TGTTTTTCAA ACTCTTGACA AGTGTAATGA TATTATCAAA 
AACAAAGATG ATACTGCAGC CTATTTGCCA ACCAAACTGG CTGCAGTAGC ATGTGTTGGG 
GCATTTTATG AAAAAATGGG GAGGATGTTA GGAAGTGCAT TTCCAGAAAC AGTTAACAAT 
CTTTTGAAGT CTCTGAAAAA TGCAGAGTCT CAAGGCCGAA GTGAAATCTT AATGAGTTTA 
CAAAAAGTCC TAAATGGACT GGGTGGTGCA GCAGCTTCTT GTCATCGTGA TATTTATAAG 
AATGCAAGAT CTCTGCTAAG TGACAGATCT ATGGCTGTTA GGTGTGCAGT TGCTAAATAC 
AGTTTGCAAC TTCTTAATGC CTCATTATTC ATATCCTCCC ATGAGTTATC CATCAAGAAT 
TTCTGCTTGC AGGCATATGT TGTTTTTGTT ACAACATTAG GTGGTCAGTG GTTAGAACGC 
AGCTTTGCTA CATTCTTATC CCATGTACTT GATCTGGTTT CACACCCTCG GGCCACTCAA 
ACACATGTAG AAGCTGTGTA TTCAAGAAGA TGTGTCTCCT TTATCCTCAG AGCTACTGTA 
GGTAGTTTGC TTGGGGAAAA AGCTCAGATT GCAGCTGCCA AAGAAATCTG CCAAGCCATT 
GGAAAACAAA TGAAAGCAGT GGAAGCAGTT GTCAATGATA CAAGTAGTGA AAACAAATCT 
GGAGCTGCTG ACATAGCAGC AAGTCAGCAT GTGATGGTGT GTGCTCTGCA GGAACTTGGA 
AGCTTAGTCC AAAGCTTAAA TGCCACTGCA TCTCCTCTTA TTCAGGAAGC ATCTATAGGT 
CTTTTGGAAA CTGTAACATC AGTGCTTCTG CATCCTAGTA TGGCTGCCCG ACTTGCTGCA 
GCATGGTGTT TGCGTTGTGT AGCTGTGGCC TTGCCTTTCC AATTGACCCC ATTTCTAGAC 
AGATGTGCAG AAAGGCTCAA TAATTTGAAG ACCTCCCCTG AAGCTGTTAG TGGTTACAGT 
TTTGCAATGG CTGCTTTATT AGGTGGAGTA CATCAGTGCC CTTTGGGTAT CCCTCATGCC 
AAAGGAAAGA TGGTAGTAAG CATTGCTGAA GATCTCTTAC GAACTGCTGC TCAGAACAGT 
AGGCTATCCT TGCAACGTAC TCAAGCTGGA TGGCTTCTAC TTGGAGCACT TATGACTTTA 
GGTCCTTCTG TTGTTCGTTA CCATCTTCCC AAAATGCTGT TATTATGGCG AAATGTCTTC 
CCTCGTTCCT TAAAGGAACT AGAAGCTGAA AAGGCACGAG GAGATTCTTT TACGTGGCAG 
GTAACATTGG AAGGCCGAGC TGGAGCTTTA TGTGCCATGA GGAGTTTTGT TGCTCATTGT 
CCTGAGCTTT TAACTGAGGA TGTGATTAGA AAATTGATGA CGCCTATTGA ATGTGCCATG 
ACAATGATGT CACACATTCC TTCTGTAATT AAAGCCCATG GAGCTCATTT GAAGGCCAGT 
GCTGCAATGG TTCGTTTAAG ACTTTACGAT ATTTTGGCAT TGTTACCCCC CAAAACTTAT 
GAAGGATCTT TTAATGCACT TCTTAGGGAG CTGGTGGCAG AATTTACTTT GACAGATAAT 
TCTGCCAATA CAACTACTTC CCTTCTTAGA TCCCTCTGCC ATTATGATGA CAGTGTTCTT 
CTTGGTTCCT GGCTTCAAGA AACTGATCAT AAATCAATTG AAGATCAGCT CCAACCAAAT 
AGTGCATCTG GAAGTGGTGC TTTAGAGCAT GACCCATCCT CCATTTATTT ACGAATACCT 
GCTGGTGAAG CTGTTCCTGG TCCTCTTCCG CTTGGAGTCT CAGTCATTGA TGCTTCTGTT 
GCTCTCTTTG GTGTCGTATT TCCTCATGTT TCTTACAAAC ACCGATTACA AATGCTGGAT 
CATTTTGCTG AGTGTGTCAA ACAGGCTAAA GGTGTTCGTC AACAGGCTGT ACAGCTTAAT 
ATTTTCACTG CTGTTCTTAG TGCATTAAAG GGCTTGGCTG AAAACAAAAG TACTTTAGGA 
CCTGAGGAAG TTCGGAAATC TGCTCTGACA TTGGTTATGG GAGCTCTCGA TAATCCAAAT 
CCCATTTTGA GATGTGCAGC TGGAGAAGCC CTTGGAAGAA TGGCTCAAGT GGTCGGAGAA 
GCAACTTTCA TTGCTAGAAT GGCTCAATAT AGCTTTGACA AATTAAAATC TGCTCGAGAT 
GTTGTTTCCA GAACTGGCCA TTCATTGGCT CTTGGCTGTC TACATCGATA TGTTGGGGGG 
ATAGGATCAG GACAACACCT CAAGACCAGT GTTAGTATTC TACTGGCACT AGCTCAAGAT 
GGAACTTCAC CTGAAGTCCA GACCTGGTCT CTTCATTCAC TTGCTTTAAT AGTGGACTCT 
AGTGGGCCAA TGTATCGTGG CTATGTTGAA CCAACCTTGT CCCTAGTCCT TACATTATTA 
TTGACAGTTC CACCTTCACA TACAGAGGTT CATCAATGTT TGGGTCGATG TTTAGGTGCT 
ATAATAACCA CCGTTGGACC TGAACTGCAG GGGAATGGAG CAACAATCTC AACAATCCGC 
TCTTCCTGTT TGGTTGGTTG TGCAATTACA CAAGACCATT CAGATTCTCT TGTTCAGGCA 
GCTGCCATAT CTTGCCTTCA ACAACTACAT ATGTTTGCAC CACGACATGT CAATCTATCT 
AGTCTGGTTC CCAGCCTTTG TGTTCATTTA TGTAGTTCCC ACTTATTACT CCGAAGGGCA 
GCAGTTGCAT GTCTTCGACA ACTTGCACAG CGAGAAGCTG CTGAAGTATG TGAATATGCT 
ATGAGCCTTG CAAAAAATGC AGGAGACAAA GAAAGCAGTG GCATCAATAT GAATCCTTTT 
GCACCAGGGG TCAGTTCTCG CCGTGATATC CATTGTCGAC ACCAAGGTGT CAATATAACA 
GAAACTGGTT TAGAAGGGCT TCTTTTTGGA ATGCTAGACC GGGAGACTGA TAGAAAATTA 
TGTTCGGATA TCCATGATAC TTTAGGACAT ATGCTTTCAT CTCTGGCTGT AGACAAACTT 
TCACATTGGC TAATGCTTTG TAAGGATGTT CTGGCAGCAT CCAGTGATAT GAGCACTACA 
ACCCCTTTAA GTAGTGGAAA GGATGAAGAA TCTGAGAAGA AGGATGAGAT GGATGATGAT 
ACTATGTTTA CCACATTAGG TGAAGAGGAT AAATCAAAGC CATCTGTAGC TCCTCGCTGG 
GCTACTCGAG TGTTTGCTGC TGACTGCTTG TGTCGAGTCA TCATGTTGTG TGAGAATGCA 
GATCAGGCTC ATTTTGATCT TGCTATGGCA CGTTCAGCCA AATTGAAAAA TCCTGCAAAT 
GATCTTCTAG TGCTCCATCT CTCTGACCTG ATACGCATGG CATTCATGGC TGCTACAGAT 
CACAGCAACC AGCTGCGGAT GGCTGGACTC CAAGCACTTG AGGACATTAT CAAGAAATTT 
GCATCCGTGC CTGAGCCAGA ATTTCCAGGT CATGTGATTT TGGAGCAGTA CCAAGCTAAT 
GTGGGAGCTG CTCTCAGACC AGCTTTTTCA CAAGATACTC CATCAGACAT AATTGCAAAA 
GCTTGCCAGG TTTGTAGTAC ATGGATTGGA AGTGGAGTTG TCAGTGATCT CAATGATCTC 
CGTCGAGTAC ACAATTTGCT TGTTTCCTCA CTTGACAAAG TACAGGCAGG AAAAGGATCT 
TCCAGCCAAC TTTATAGAGA GAGTGCAACA ACCATGGAAA AATTGGCAGT GCTAAAAGCT 
TGGGCTGAGG TTTATGTTGT TGCCATGAAT ATTAAAAAGG AAGCAGAAAC TAAACCAAAA 
AGAGCCATAA AAAATACTGA TGATGAAGAT GATGATTATG GGACCATAGA TGAGTTGCCA 
CCAGACAGTT TAATTACACT TGTACAACCT GAATTGCCAA CACTTAGCCG CCTCTGGTTG 
GCAGCATTAA AAGATTATGC ACTTTTGACT TTACCAGCTG AATTTTCAAG TCAGCTTCCA 
CCAGATGGGG GAGCATTTTA TACTCCAGAG ACGATTGATA CGGCTCGACT TCACTATCGG 
AACTCTTGGG CTCCAATTCT CCATGCAGTA GCACTTTGGT TAAATAGCAC AGGATTTACT 
AATTCAGAAG CAACAGAAGA AACATCAGTA TCTGGTTTGC AAAAACGTCC AACACCTGTC 
ATTTTAAACC AAATACCTGG AGCAACAGCT AATGCTAAAT CTTTGCCAGA AATCAACAAG 
GACAGAATGC ATCTGATTTT AGGTGTTAGT ATACAGTTTC TCTGTTCCCC AAGACCTGAG 
GAACCTATTG AACATGTTAC AGCCTGTCTG CAGGCATTGC ATACCTTGCT GGAATCCCCT 
TGTGCCAGAA TTCATATTGG AGAAGATCAG CTCATTGGTG TTGAGTTGCT GAATGTGCTA 
CATCGACTCC TTTTGACTTG GAATCCACCC TCAGTTCAGC TGTTGGTTAC TGGAGTGGTA 
CAGCAGATAG TTAGAGCAGC TCAAGATTAT TTGCAAGAAA AAAGAAACAC TCTAAATGAA 
GATGATATGG AAAAAGAGAG TTGCCCTGTT CTAGGAGAAG GAGGTGACAA TGGTGGTCTT 
GTGCCTGGAA AATCTCTTGT CTTTGCAACC ATGGAGCTAT TGATGTTCAT CTTAGTACGG 
CACATGCCAC ATCTCAGTAC CAAGATGTCA GATTCACCAA GTCATATATC CACTAAAACT 
CGATTATCTG AAGAAAGTGC TCGTTTAGTG GCAGCGGCAG TTACCATCCT CTCAGACTTG 
CCATCTCTTT GCTCTCCTGC TGGATGTATG ACAATATTAC CCACAATTCT CTTTCTGATT 
GCAAGAATAT TGAAAGATAC TGCAATAAAG TCTGGTGATA ATCAAGTACC CCCTCCAGTT 
AATGCAGCTC TACAAGGAAT TAAAAACATT GTGACTCTCT CAATGGCCAA AACTGATGAA 
ACGCAAAAAC AGTGGACTGA TCTAATTCGT AGCACTCTTG CATGTATCCT GGAATATTCT 
CAACCAGAAG ATTCGATGCC AATTCCCGAT GAAGTGAGCA TGCTTACAGC CATTGCACTC 
TTTCTATGGT CTGCTAGTAC TGAAATAATT GGAGTTCAGT CATTACAGAA TGGTTGCATG 
AACAGATTTA AAAATGCACT GAATTCTTGT GATCCTTGGG TTCAAGCTAA GTGTTACCAG 
CTTCTTCTTT CAGTCTTCCA GCATTCCAAC CGTGCCCTTT CAACTCCCTA CATCCATTCA 
TTAGCTCCAA TAATGGTTGA AAAACTAAAA GCTGTTGAAA GGAGCCGGCC AACCAGTAGT 
ACAGAGCTTT TAGCTGTTCA AGAAGGAATC AAAGTTCTTG AAACATTGGT TGCCCTTGGT 
GAAGAGCAGA ACAGAGTCCA GCTACTTGCT CTTTTAGTTC CAACTTTGAT TTCATATTTG 
TTAGATGAAA ATGCTTTTGA TTCAGCACTT ACGGCATCCA AAGATCTTCA TGAATTTGCA 
CTCCAGAATT TAATGCACAT TGGGCCTCTC TATCCACATG CTTTCAAGAC TGTTATGGGA 
GCTGCTCCTG AATTGAAAAC AAGACTCGAA ACTGCAGTTC GCGCAAGTCA AGCTAGTAAA 
GCAAAAGCAG CTGCCAGGCA ACCAGCACCT ACCATACATT CCGCTCCAAC AATTAAACTC 
AAAACAAGCT TTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5B ( XM_031951273.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031807133.1 Sarcophilus harrisii HEAT repeat containing 5B (HEATR5B), transcript variant X4, mRNA.

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CloneID	OSa224489
Clone ID Related Accession (Same CDS sequence)	XM_031951273.1
Accession Version	XM_031951273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	HEAT repeat-containing protein 5B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGG CCCATAGTTT ATTGCTAAAT GAAGAAGCTT TGGCCCAAAT TACAGAAGCA 
AAGAGGCCTG TTTTTATCTT TGAGTGGTTG CGATTTCTTG ACAAAGTCCT GGTAGCTGCT 
AACAAGACTG ATGTGAAGGA AAAACAGAAA AGGCTTGTTG AACAACTCAC TGGATTAATA 
AGTAGTTCTC CTGGCCCCCC AACAAGGAAA TTACTAGCTA AAAATCTTGC AGCTCTTTAT 
AGCATTGGAG ATACTTTCAC TGTTTTTCAA ACTCTTGACA AGTGTAATGA TATTATCAAA 
AACAAAGATG ATACTGCAGC CTATTTGCCA ACCAAACTGG CTGCAGTAGC ATGTGTTGGG 
GCATTTTATG AAAAAATGGG GAGGATGTTA GGAAGTGCAT TTCCAGAAAC AGTTAACAAT 
CTTTTGAAGT CTCTGAAAAA TGCAGAGTCT CAAGGCCGAA GTGAAATCTT AATGAGTTTA 
CAAAAAGTCC TAAATGGACT GGGTGGTGCA GCAGCTTCTT GTCATCGTGA TATTTATAAG 
AATGCAAGAT CTCTGCTAAG TGACAGATCT ATGGCTGTTA GGTGTGCAGT TGCTAAATGT 
CTGCTAGAAC TACAAAATGA AGCTGTATTT ATGTGGACAG CTGAATTAGA AAATATAGCC 
ACATTATCTT TTAAGGCTTT GGAAAGCTCA AATTATGGGG TTCGAGTGGC AGTGGCAAAA 
CTCTTGGGAA CAGTCATGGC AACTGCATTG ATGCCTAAAC AGGCAATGGT AATGCGCCAG 
AATGTGAAAA GAGCCACTTT AGATGAAGTC TTAGAGCTCA TGGCCACAGG ATTTCTGCGA 
GGAGGGTCTG GATTCTTAAA GAGTGGTGGA GAAATGCTAA AAGTTGGTGG GTCAGTTAAT 
CGTGAAGTAA GAGTTGGAGT TACACAGGCA TATGTTGTTT TTGTTACAAC ATTAGGTGGT 
CAGTGGTTAG AACGCAGCTT TGCTACATTC TTATCCCATG TACTTGATCT GGTTTCACAC 
CCTCGGGCCA CTCAAACACA TGTAGAAGCT GTGTATTCAA GAAGATGTGT CTCCTTTATC 
CTCAGAGCTA CTGTAGGTAG TTTGCTTGGG GAAAAAGCTC AGATTGCAGC TGCCAAAGAA 
ATCTGCCAAG CCATTGGAAA ACAAATGAAA GCAGTGGAAG CAGTTGTCAA TGATACAAGT 
AGTGAAAACA AATCTGGAGC TGCTGACATA GCAGCAAGTC AGCATGTGAT GGTGTGTGCT 
CTGCAGGAAC TTGGAAGCTT AGTCCAAAGC TTAAATGCCA CTGCATCTCC TCTTATTCAG 
GAAGCATCTA TAGGTCTTTT GGAAACTGTA ACATCAGTGC TTCTGCATCC TAGTATGGCT 
GCCCGACTTG CTGCAGCATG GTGTTTGCGT TGTGTAGCTG TGGCCTTGCC TTTCCAATTG 
ACCCCATTTC TAGACAGATG TGCAGAAAGG CTCAATAATT TGAAGACCTC CCCTGAAGCT 
GTTAGTGGTT ACAGTTTTGC AATGGCTGCT TTATTAGGTG GAGTACATCA GTGCCCTTTG 
GGTATCCCTC ATGCCAAAGG AAAGATGGTA GTAAGCATTG CTGAAGATCT CTTACGAACT 
GCTGCTCAGA ACAGTAGGCT ATCCTTGCAA CGTACTCAAG CTGGATGGCT TCTACTTGGA 
GCACTTATGA CTTTAGGTCC TTCTGTTGTT CGTTACCATC TTCCCAAAAT GCTGTTATTA 
TGGCGAAATG TCTTCCCTCG TTCCTTAAAG GAACTAGAAG CTGAAAAGGC ACGAGGAGAT 
TCTTTTACGT GGCAGGTAAC ATTGGAAGGC CGAGCTGGAG CTTTATGTGC CATGAGGAGT 
TTTGTTGCTC ATTGTCCTGA GCTTTTAACT GAGGATGTGA TTAGAAAATT GATGACGCCT 
ATTGAATGTG CCATGACAAT GATGTCACAC ATTCCTTCTG TAATTAAAGC CCATGGAGCT 
CATTTGAAGG CCAGTGCTGC AATGGTTCGT TTAAGACTTT ACGATATTTT GGCATTGTTA 
CCCCCCAAAA CTTATGAAGG ATCTTTTAAT GCACTTCTTA GGGAGCTGGT GGCAGAATTT 
ACTTTGACAG ATAATTCTGC CAATACAACT ACTTCCCTTC TTAGATCCCT CTGCCATTAT 
GATGACAGTG TTCTTCTTGG TTCCTGGCTT CAAGAAACTG ATCATAAATC AATTGAAGAT 
CAGCTCCAAC CAAATAGTGC ATCTGGAAGT GGTGCTTTAG AGCATGACCC ATCCTCCATT 
TATTTACGAA TACCTGCTGG TGAAGCTGTT CCTGGTCCTC TTCCGCTTGG AGTCTCAGTC 
ATTGATGCTT CTGTTGCTCT CTTTGGTGTC GTATTTCCTC ATGTTTCTTA CAAACACCGA 
TTACAAATGC TGGATCATTT TGCTGAGTGT GTCAAACAGG CTAAAGGTGT TCGTCAACAG 
GCTGTACAGC TTAATATTTT CACTGCTGTT CTTAGTGCAT TAAAGGGCTT GGCTGAAAAC 
AAAAGTACTT TAGGACCTGA GGAAGTTCGG AAATCTGCTC TGACATTGGT TATGGGAGCT 
CTCGATAATC CAAATCCCAT TTTGAGATGT GCAGCTGGAG AAGCCCTTGG AAGAATGGCT 
CAAGTGGTCG GAGAAGCAAC TTTCATTGCT AGAATGGCTC AATATAGCTT TGACAAATTA 
AAATCTGCTC GAGATGTTGT TTCCAGAACT GGCCATTCAT TGGCTCTTGG CTGTCTACAT 
CGATATGTTG GGGGGATAGG ATCAGGACAA CACCTCAAGA CCAGTGTTAG TATTCTACTG 
GCACTAGCTC AAGATGGAAC TTCACCTGAA GTCCAGACCT GGTCTCTTCA TTCACTTGCT 
TTAATAGTGG ACTCTAGTGG GCCAATGTAT CGTGGCTATG TTGAACCAAC CTTGTCCCTA 
GTCCTTACAT TATTATTGAC AGTTCCACCT TCACATACAG AGGTTCATCA ATGTTTGGGT 
CGATGTTTAG GTGCTATAAT AACCACCGTT GGACCTGAAC TGCAGGGGAA TGGAGCAACA 
ATCTCAACAA TCCGCTCTTC CTGTTTGGTT GGTTGTGCAA TTACACAAGA CCATTCAGAT 
TCTCTTGTTC AGGCAGCTGC CATATCTTGC CTTCAACAAC TACATATGTT TGCACCACGA 
CATGTCAATC TATCTAGTCT GGTTCCCAGC CTTTGTGTTC ATTTATGTAG TTCCCACTTA 
TTACTCCGAA GGGCAGCAGT TGCATGTCTT CGACAACTTG CACAGCGAGA AGCTGCTGAA 
GTATGTGAAT ATGCTATGAG CCTTGCAAAA AATGCAGGAG ACAAAGAAAG CAGTGGCATC 
AATATGAATC CTTTTGCACC AGGGGTCAGT TCTCGCCGTG ATATCCATTG TCGACACCAA 
GGTGTCAATA TAACAGAAAC TGGTTTAGAA GGGCTTCTTT TTGGAATGCT AGACCGGGAG 
ACTGATAGAA AATTATGTTC GGATATCCAT GATACTTTAG GACATATGCT TTCATCTCTG 
GCTGTAGACA AACTTTCACA TTGGCTAATG CTTTGTAAGG ATGTTCTGGC AGCATCCAGT 
GATATGAGCA CTACAACCCC TTTAAGTAGT GGAAAGGATG AAGAATCTGA GAAGAAGGAT 
GAGATGGATG ATGATACTAT GTTTACCACA TTAGGTGAAG AGGATAAATC AAAGCCATCT 
GTAGCTCCTC GCTGGGCTAC TCGAGTGTTT GCTGCTGACT GCTTGTGTCG AGTCATCATG 
TTGTGTGAGA ATGCAGATCA GGCTCATTTT GATCTTGCTA TGGCACGTTC AGCCAAATTG 
AAAAATCCTG CAAATGATCT TCTAGTGCTC CATCTCTCTG ACCTGATACG CATGGCATTC 
ATGGCTGCTA CAGATCACAG CAACCAGCTG CGGATGGCTG GACTCCAAGC ACTTGAGGAC 
ATTATCAAGA AATTTGCATC CGTGCCTGAG CCAGAATTTC CAGGTCATGT GATTTTGGAG 
CAGTACCAAG CTAATGTGGG AGCTGCTCTC AGACCAGCTT TTTCACAAGA TACTCCATCA 
GACATAATTG CAAAAGCTTG CCAGGTTTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT TTGCTTGTTT CCTCACTTGA CAAAGTACAG 
GCAGGAAAAG GATCTTCCAG CCAACTTTAT AGAGAGAGTG CAACAACCAT GGAAAAATTG 
GCAGTGCTAA AAGCTTGGGC TGAGGTTTAT GTTGTTGCCA TGAATATTAA AAAGGAAGCA 
GAAACTAAAC CAAAAAGAGC CATAAAAAAT ACTGATGATG AAGATGATGA TTATGGGACC 
ATAGATGAGT TGCCACCAGA CAGTTTAATT ACACTTGTAC AACCTGAATT GCCAACACTT 
AGCCGCCTCT GGTTGGCAGC ATTAAAAGAT TATGCACTTT TGACTTTACC AGCTGAATTT 
TCAAGTCAGC TTCCACCAGA TGGGGGAGCA TTTTATACTC CAGAGACGAT TGATACGGCT 
CGACTTCACT ATCGGAACTC TTGGGCTCCA ATTCTCCATG CAGTAGCACT TTGGTTAAAT 
AGCACAGGAT TTACTAATTC AGAAGCAACA GAAGAAACAT CAGTATCTGG TTTGCAAAAA 
CGTCCAACAC CTGTCATTTT AAACCAAATA CCTGGAGCAA CAGCTAATGC TAAATCTTTG 
CCAGAAATCA ACAAGGACAG AATGCATCTG ATTTTAGGTG TTAGTATACA GTTTCTCTGT 
TCCCCAAGAC CTGAGGAACC TATTGAACAT GTTACAGCCT GTCTGCAGGC ATTGCATACC 
TTGCTGGAAT CCCCTTGTGC CAGAATTCAT ATTGGAGAAG ATCAGCTCAT TGGTGTTGAG 
TTGCTGAATG TGCTACATCG ACTCCTTTTG ACTTGGAATC CACCCTCAGT TCAGCTGTTG 
GTTACTGGAG TGGTACAGCA GATAGTTAGA GCAGCTCAAG ATTATTTGCA AGAAAAAAGA 
AACACTCTAA ATGAAGATGA TATGGAAAAA GAGAGTTGCC CTGTTCTAGG AGAAGGAGGT 
GACAATGGTG GTCTTGTGCC TGGAAAATCT CTTGTCTTTG CAACCATGGA GCTATTGATG 
TTCATCTTAG TACGGCACAT GCCACATCTC AGTACCAAGA TGTCAGATTC ACCAAGTCAT 
ATATCCACTA AAACTCGATT ATCTGAAGAA AGTGCTCGTT TAGTGGCAGC GGCAGTTACC 
ATCCTCTCAG ACTTGCCATC TCTTTGCTCT CCTGCTGGTA TAGTAGTTCT TAATATATAC 
AAGATGTATG ACAATATTAC CCACAATTCT CTTTCTGATT GCAAGAATAT TGAAAGATAC 
TGCAATAAAG TCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031807133.1
CDS	175..5592
Translation	MELAHSLLLNEEALAQITEAKRPVFIFEWLRFLDKVLVAANKTDVKEKQKRLVEQLTGLISSSPGPPTRKLLAKNLAALYSIGDTFTVFQTLDKCNDIIKNKDDTAAYLPTKLAAVACVGAFYEKMGRMLGSAFPETVNNLLKSLKNAESQGRSEILMSLQKVLNGLGGAAASCHRDIYKNARSLLSDRSMAVRCAVAKCLLELQNEAVFMWTAELENIATLSFKALESSNYGVRVAVAKLLGTVMATALMPKQAMVMRQNVKRATLDEVLELMATGFLRGGSGFLKSGGEMLKVGGSVNREVRVGVTQAYVVFVTTLGGQWLERSFATFLSHVLDLVSHPRATQTHVEAVYSRRCVSFILRATVGSLLGEKAQIAAAKEICQAIGKQMKAVEAVVNDTSSENKSGAADIAASQHVMVCALQELGSLVQSLNATASPLIQEASIGLLETVTSVLLHPSMAARLAAAWCLRCVAVALPFQLTPFLDRCAERLNNLKTSPEAVSGYSFAMAALLGGVHQCPLGIPHAKGKMVVSIAEDLLRTAAQNSRLSLQRTQAGWLLLGALMTLGPSVVRYHLPKMLLLWRNVFPRSLKELEAEKARGDSFTWQVTLEGRAGALCAMRSFVAHCPELLTEDVIRKLMTPIECAMTMMSHIPSVIKAHGAHLKASAAMVRLRLYDILALLPPKTYEGSFNALLRELVAEFTLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAVPGPLPLGVSVIDASVALFGVVFPHVSYKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAENKSTLGPEEVRKSALTLVMGALDNPNPILRCAAGEALGRMAQVVGEATFIARMAQYSFDKLKSARDVVSRTGHSLALGCLHRYVGGIGSGQHLKTSVSILLALAQDGTSPEVQTWSLHSLALIVDSSGPMYRGYVEPTLSLVLTLLLTVPPSHTEVHQCLGRCLGAIITTVGPELQGNGATISTIRSSCLVGCAITQDHSDSLVQAAAISCLQQLHMFAPRHVNLSSLVPSLCVHLCSSHLLLRRAAVACLRQLAQREAAEVCEYAMSLAKNAGDKESSGINMNPFAPGVSSRRDIHCRHQGVNITETGLEGLLFGMLDRETDRKLCSDIHDTLGHMLSSLAVDKLSHWLMLCKDVLAASSDMSTTTPLSSGKDEESEKKDEMDDDTMFTTLGEEDKSKPSVAPRWATRVFAADCLCRVIMLCENADQAHFDLAMARSAKLKNPANDLLVLHLSDLIRMAFMAATDHSNQLRMAGLQALEDIIKKFASVPEPEFPGHVILEQYQANVGAALRPAFSQDTPSDIIAKACQVCSTWIGSGVVSDLNDLRRVHNLLVSSLDKVQAGKGSSSQLYRESATTMEKLAVLKAWAEVYVVAMNIKKEAETKPKRAIKNTDDEDDDYGTIDELPPDSLITLVQPELPTLSRLWLAALKDYALLTLPAEFSSQLPPDGGAFYTPETIDTARLHYRNSWAPILHAVALWLNSTGFTNSEATEETSVSGLQKRPTPVILNQIPGATANAKSLPEINKDRMHLILGVSIQFLCSPRPEEPIEHVTACLQALHTLLESPCARIHIGEDQLIGVELLNVLHRLLLTWNPPSVQLLVTGVVQQIVRAAQDYLQEKRNTLNEDDMEKESCPVLGEGGDNGGLVPGKSLVFATMELLMFILVRHMPHLSTKMSDSPSHISTKTRLSEESARLVAAAVTILSDLPSLCSPAGIVVLNIYKMYDNITHNSLSDCKNIERYCNKVW

Target ORF information:

RefSeq Version	XM_031951273.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HEAT repeat containing 5B (HEATR5B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031951273.1

ORF Insert Sequence:

ATGGAGTTGG CCCATAGTTT ATTGCTAAAT GAAGAAGCTT TGGCCCAAAT TACAGAAGCA 
AAGAGGCCTG TTTTTATCTT TGAGTGGTTG CGATTTCTTG ACAAAGTCCT GGTAGCTGCT 
AACAAGACTG ATGTGAAGGA AAAACAGAAA AGGCTTGTTG AACAACTCAC TGGATTAATA 
AGTAGTTCTC CTGGCCCCCC AACAAGGAAA TTACTAGCTA AAAATCTTGC AGCTCTTTAT 
AGCATTGGAG ATACTTTCAC TGTTTTTCAA ACTCTTGACA AGTGTAATGA TATTATCAAA 
AACAAAGATG ATACTGCAGC CTATTTGCCA ACCAAACTGG CTGCAGTAGC ATGTGTTGGG 
GCATTTTATG AAAAAATGGG GAGGATGTTA GGAAGTGCAT TTCCAGAAAC AGTTAACAAT 
CTTTTGAAGT CTCTGAAAAA TGCAGAGTCT CAAGGCCGAA GTGAAATCTT AATGAGTTTA 
CAAAAAGTCC TAAATGGACT GGGTGGTGCA GCAGCTTCTT GTCATCGTGA TATTTATAAG 
AATGCAAGAT CTCTGCTAAG TGACAGATCT ATGGCTGTTA GGTGTGCAGT TGCTAAATGT 
CTGCTAGAAC TACAAAATGA AGCTGTATTT ATGTGGACAG CTGAATTAGA AAATATAGCC 
ACATTATCTT TTAAGGCTTT GGAAAGCTCA AATTATGGGG TTCGAGTGGC AGTGGCAAAA 
CTCTTGGGAA CAGTCATGGC AACTGCATTG ATGCCTAAAC AGGCAATGGT AATGCGCCAG 
AATGTGAAAA GAGCCACTTT AGATGAAGTC TTAGAGCTCA TGGCCACAGG ATTTCTGCGA 
GGAGGGTCTG GATTCTTAAA GAGTGGTGGA GAAATGCTAA AAGTTGGTGG GTCAGTTAAT 
CGTGAAGTAA GAGTTGGAGT TACACAGGCA TATGTTGTTT TTGTTACAAC ATTAGGTGGT 
CAGTGGTTAG AACGCAGCTT TGCTACATTC TTATCCCATG TACTTGATCT GGTTTCACAC 
CCTCGGGCCA CTCAAACACA TGTAGAAGCT GTGTATTCAA GAAGATGTGT CTCCTTTATC 
CTCAGAGCTA CTGTAGGTAG TTTGCTTGGG GAAAAAGCTC AGATTGCAGC TGCCAAAGAA 
ATCTGCCAAG CCATTGGAAA ACAAATGAAA GCAGTGGAAG CAGTTGTCAA TGATACAAGT 
AGTGAAAACA AATCTGGAGC TGCTGACATA GCAGCAAGTC AGCATGTGAT GGTGTGTGCT 
CTGCAGGAAC TTGGAAGCTT AGTCCAAAGC TTAAATGCCA CTGCATCTCC TCTTATTCAG 
GAAGCATCTA TAGGTCTTTT GGAAACTGTA ACATCAGTGC TTCTGCATCC TAGTATGGCT 
GCCCGACTTG CTGCAGCATG GTGTTTGCGT TGTGTAGCTG TGGCCTTGCC TTTCCAATTG 
ACCCCATTTC TAGACAGATG TGCAGAAAGG CTCAATAATT TGAAGACCTC CCCTGAAGCT 
GTTAGTGGTT ACAGTTTTGC AATGGCTGCT TTATTAGGTG GAGTACATCA GTGCCCTTTG 
GGTATCCCTC ATGCCAAAGG AAAGATGGTA GTAAGCATTG CTGAAGATCT CTTACGAACT 
GCTGCTCAGA ACAGTAGGCT ATCCTTGCAA CGTACTCAAG CTGGATGGCT TCTACTTGGA 
GCACTTATGA CTTTAGGTCC TTCTGTTGTT CGTTACCATC TTCCCAAAAT GCTGTTATTA 
TGGCGAAATG TCTTCCCTCG TTCCTTAAAG GAACTAGAAG CTGAAAAGGC ACGAGGAGAT 
TCTTTTACGT GGCAGGTAAC ATTGGAAGGC CGAGCTGGAG CTTTATGTGC CATGAGGAGT 
TTTGTTGCTC ATTGTCCTGA GCTTTTAACT GAGGATGTGA TTAGAAAATT GATGACGCCT 
ATTGAATGTG CCATGACAAT GATGTCACAC ATTCCTTCTG TAATTAAAGC CCATGGAGCT 
CATTTGAAGG CCAGTGCTGC AATGGTTCGT TTAAGACTTT ACGATATTTT GGCATTGTTA 
CCCCCCAAAA CTTATGAAGG ATCTTTTAAT GCACTTCTTA GGGAGCTGGT GGCAGAATTT 
ACTTTGACAG ATAATTCTGC CAATACAACT ACTTCCCTTC TTAGATCCCT CTGCCATTAT 
GATGACAGTG TTCTTCTTGG TTCCTGGCTT CAAGAAACTG ATCATAAATC AATTGAAGAT 
CAGCTCCAAC CAAATAGTGC ATCTGGAAGT GGTGCTTTAG AGCATGACCC ATCCTCCATT 
TATTTACGAA TACCTGCTGG TGAAGCTGTT CCTGGTCCTC TTCCGCTTGG AGTCTCAGTC 
ATTGATGCTT CTGTTGCTCT CTTTGGTGTC GTATTTCCTC ATGTTTCTTA CAAACACCGA 
TTACAAATGC TGGATCATTT TGCTGAGTGT GTCAAACAGG CTAAAGGTGT TCGTCAACAG 
GCTGTACAGC TTAATATTTT CACTGCTGTT CTTAGTGCAT TAAAGGGCTT GGCTGAAAAC 
AAAAGTACTT TAGGACCTGA GGAAGTTCGG AAATCTGCTC TGACATTGGT TATGGGAGCT 
CTCGATAATC CAAATCCCAT TTTGAGATGT GCAGCTGGAG AAGCCCTTGG AAGAATGGCT 
CAAGTGGTCG GAGAAGCAAC TTTCATTGCT AGAATGGCTC AATATAGCTT TGACAAATTA 
AAATCTGCTC GAGATGTTGT TTCCAGAACT GGCCATTCAT TGGCTCTTGG CTGTCTACAT 
CGATATGTTG GGGGGATAGG ATCAGGACAA CACCTCAAGA CCAGTGTTAG TATTCTACTG 
GCACTAGCTC AAGATGGAAC TTCACCTGAA GTCCAGACCT GGTCTCTTCA TTCACTTGCT 
TTAATAGTGG ACTCTAGTGG GCCAATGTAT CGTGGCTATG TTGAACCAAC CTTGTCCCTA 
GTCCTTACAT TATTATTGAC AGTTCCACCT TCACATACAG AGGTTCATCA ATGTTTGGGT 
CGATGTTTAG GTGCTATAAT AACCACCGTT GGACCTGAAC TGCAGGGGAA TGGAGCAACA 
ATCTCAACAA TCCGCTCTTC CTGTTTGGTT GGTTGTGCAA TTACACAAGA CCATTCAGAT 
TCTCTTGTTC AGGCAGCTGC CATATCTTGC CTTCAACAAC TACATATGTT TGCACCACGA 
CATGTCAATC TATCTAGTCT GGTTCCCAGC CTTTGTGTTC ATTTATGTAG TTCCCACTTA 
TTACTCCGAA GGGCAGCAGT TGCATGTCTT CGACAACTTG CACAGCGAGA AGCTGCTGAA 
GTATGTGAAT ATGCTATGAG CCTTGCAAAA AATGCAGGAG ACAAAGAAAG CAGTGGCATC 
AATATGAATC CTTTTGCACC AGGGGTCAGT TCTCGCCGTG ATATCCATTG TCGACACCAA 
GGTGTCAATA TAACAGAAAC TGGTTTAGAA GGGCTTCTTT TTGGAATGCT AGACCGGGAG 
ACTGATAGAA AATTATGTTC GGATATCCAT GATACTTTAG GACATATGCT TTCATCTCTG 
GCTGTAGACA AACTTTCACA TTGGCTAATG CTTTGTAAGG ATGTTCTGGC AGCATCCAGT 
GATATGAGCA CTACAACCCC TTTAAGTAGT GGAAAGGATG AAGAATCTGA GAAGAAGGAT 
GAGATGGATG ATGATACTAT GTTTACCACA TTAGGTGAAG AGGATAAATC AAAGCCATCT 
GTAGCTCCTC GCTGGGCTAC TCGAGTGTTT GCTGCTGACT GCTTGTGTCG AGTCATCATG 
TTGTGTGAGA ATGCAGATCA GGCTCATTTT GATCTTGCTA TGGCACGTTC AGCCAAATTG 
AAAAATCCTG CAAATGATCT TCTAGTGCTC CATCTCTCTG ACCTGATACG CATGGCATTC 
ATGGCTGCTA CAGATCACAG CAACCAGCTG CGGATGGCTG GACTCCAAGC ACTTGAGGAC 
ATTATCAAGA AATTTGCATC CGTGCCTGAG CCAGAATTTC CAGGTCATGT GATTTTGGAG 
CAGTACCAAG CTAATGTGGG AGCTGCTCTC AGACCAGCTT TTTCACAAGA TACTCCATCA 
GACATAATTG CAAAAGCTTG CCAGGTTTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT TTGCTTGTTT CCTCACTTGA CAAAGTACAG 
GCAGGAAAAG GATCTTCCAG CCAACTTTAT AGAGAGAGTG CAACAACCAT GGAAAAATTG 
GCAGTGCTAA AAGCTTGGGC TGAGGTTTAT GTTGTTGCCA TGAATATTAA AAAGGAAGCA 
GAAACTAAAC CAAAAAGAGC CATAAAAAAT ACTGATGATG AAGATGATGA TTATGGGACC 
ATAGATGAGT TGCCACCAGA CAGTTTAATT ACACTTGTAC AACCTGAATT GCCAACACTT 
AGCCGCCTCT GGTTGGCAGC ATTAAAAGAT TATGCACTTT TGACTTTACC AGCTGAATTT 
TCAAGTCAGC TTCCACCAGA TGGGGGAGCA TTTTATACTC CAGAGACGAT TGATACGGCT 
CGACTTCACT ATCGGAACTC TTGGGCTCCA ATTCTCCATG CAGTAGCACT TTGGTTAAAT 
AGCACAGGAT TTACTAATTC AGAAGCAACA GAAGAAACAT CAGTATCTGG TTTGCAAAAA 
CGTCCAACAC CTGTCATTTT AAACCAAATA CCTGGAGCAA CAGCTAATGC TAAATCTTTG 
CCAGAAATCA ACAAGGACAG AATGCATCTG ATTTTAGGTG TTAGTATACA GTTTCTCTGT 
TCCCCAAGAC CTGAGGAACC TATTGAACAT GTTACAGCCT GTCTGCAGGC ATTGCATACC 
TTGCTGGAAT CCCCTTGTGC CAGAATTCAT ATTGGAGAAG ATCAGCTCAT TGGTGTTGAG 
TTGCTGAATG TGCTACATCG ACTCCTTTTG ACTTGGAATC CACCCTCAGT TCAGCTGTTG 
GTTACTGGAG TGGTACAGCA GATAGTTAGA GCAGCTCAAG ATTATTTGCA AGAAAAAAGA 
AACACTCTAA ATGAAGATGA TATGGAAAAA GAGAGTTGCC CTGTTCTAGG AGAAGGAGGT 
GACAATGGTG GTCTTGTGCC TGGAAAATCT CTTGTCTTTG CAACCATGGA GCTATTGATG 
TTCATCTTAG TACGGCACAT GCCACATCTC AGTACCAAGA TGTCAGATTC ACCAAGTCAT 
ATATCCACTA AAACTCGATT ATCTGAAGAA AGTGCTCGTT TAGTGGCAGC GGCAGTTACC 
ATCCTCTCAG ACTTGCCATC TCTTTGCTCT CCTGCTGGTA TAGTAGTTCT TAATATATAC 
AAGATGTATG ACAATATTAC CCACAATTCT CTTTCTGATT GCAAGAATAT TGAAAGATAC 
TGCAATAAAG TCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5B ( XM_031951270.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031807130.1 Sarcophilus harrisii HEAT repeat containing 5B (HEATR5B), transcript variant X1, mRNA.

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CloneID	OSa224487
Clone ID Related Accession (Same CDS sequence)	XM_031951270.1 , XM_031951272.1
Accession Version	XM_031951270.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	HEAT repeat-containing protein 5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGG CCCATAGTTT ATTGCTAAAT GAAGAAGCTT TGGCCCAAAT TACAGAAGCA 
AAGAGGCCTG TTTTTATCTT TGAGTGGTTG CGATTTCTTG ACAAAGTCCT GGTAGCTGCT 
AACAAGACTG ATGTGAAGGA AAAACAGAAA AGGCTTGTTG AACAACTCAC TGGATTAATA 
AGTAGTTCTC CTGGCCCCCC AACAAGGAAA TTACTAGCTA AAAATCTTGC AGCTCTTTAT 
AGCATTGGAG ATACTTTCAC TGTTTTTCAA ACTCTTGACA AGTGTAATGA TATTATCAAA 
AACAAAGATG ATACTGCAGC CTATTTGCCA ACCAAACTGG CTGCAGTAGC ATGTGTTGGG 
GCATTTTATG AAAAAATGGG GAGGATGTTA GGAAGTGCAT TTCCAGAAAC AGTTAACAAT 
CTTTTGAAGT CTCTGAAAAA TGCAGAGTCT CAAGGCCGAA GTGAAATCTT AATGAGTTTA 
CAAAAAGTCC TAAATGGACT GGGTGGTGCA GCAGCTTCTT GTCATCGTGA TATTTATAAG 
AATGCAAGAT CTCTGCTAAG TGACAGATCT ATGGCTGTTA GGTGTGCAGT TGCTAAATGT 
CTGCTAGAAC TACAAAATGA AGCTGTATTT ATGTGGACAG CTGAATTAGA AAATATAGCC 
ACATTATCTT TTAAGGCTTT GGAAAGCTCA AATTATGGGG TTCGAGTGGC AGTGGCAAAA 
CTCTTGGGAA CAGTCATGGC AACTGCATTG ATGCCTAAAC AGGCAATGGT AATGCGCCAG 
AATGTGAAAA GAGCCACTTT AGATGAAGTC TTAGAGCTCA TGGCCACAGG ATTTCTGCGA 
GGAGGGTCTG GATTCTTAAA GAGTGGTGGA GAAATGCTAA AAGTTGGTGG GTCAGTTAAT 
CGTGAAGTAA GAGTTGGAGT TACACAGGCA TATGTTGTTT TTGTTACAAC ATTAGGTGGT 
CAGTGGTTAG AACGCAGCTT TGCTACATTC TTATCCCATG TACTTGATCT GGTTTCACAC 
CCTCGGGCCA CTCAAACACA TGTAGAAGCT GTGTATTCAA GAAGATGTGT CTCCTTTATC 
CTCAGAGCTA CTGTAGGTAG TTTGCTTGGG GAAAAAGCTC AGATTGCAGC TGCCAAAGAA 
ATCTGCCAAG CCATTGGAAA ACAAATGAAA GCAGTGGAAG CAGTTGTCAA TGATACAAGT 
AGTGAAAACA AATCTGGAGC TGCTGACATA GCAGCAAGTC AGCATGTGAT GGTGTGTGCT 
CTGCAGGAAC TTGGAAGCTT AGTCCAAAGC TTAAATGCCA CTGCATCTCC TCTTATTCAG 
GAAGCATCTA TAGGTCTTTT GGAAACTGTA ACATCAGTGC TTCTGCATCC TAGTATGGCT 
GCCCGACTTG CTGCAGCATG GTGTTTGCGT TGTGTAGCTG TGGCCTTGCC TTTCCAATTG 
ACCCCATTTC TAGACAGATG TGCAGAAAGG CTCAATAATT TGAAGACCTC CCCTGAAGCT 
GTTAGTGGTT ACAGTTTTGC AATGGCTGCT TTATTAGGTG GAGTACATCA GTGCCCTTTG 
GGTATCCCTC ATGCCAAAGG AAAGATGGTA GTAAGCATTG CTGAAGATCT CTTACGAACT 
GCTGCTCAGA ACAGTAGGCT ATCCTTGCAA CGTACTCAAG CTGGATGGCT TCTACTTGGA 
GCACTTATGA CTTTAGGTCC TTCTGTTGTT CGTTACCATC TTCCCAAAAT GCTGTTATTA 
TGGCGAAATG TCTTCCCTCG TTCCTTAAAG GAACTAGAAG CTGAAAAGGC ACGAGGAGAT 
TCTTTTACGT GGCAGGTAAC ATTGGAAGGC CGAGCTGGAG CTTTATGTGC CATGAGGAGT 
TTTGTTGCTC ATTGTCCTGA GCTTTTAACT GAGGATGTGA TTAGAAAATT GATGACGCCT 
ATTGAATGTG CCATGACAAT GATGTCACAC ATTCCTTCTG TAATTAAAGC CCATGGAGCT 
CATTTGAAGG CCAGTGCTGC AATGGTTCGT TTAAGACTTT ACGATATTTT GGCATTGTTA 
CCCCCCAAAA CTTATGAAGG ATCTTTTAAT GCACTTCTTA GGGAGCTGGT GGCAGAATTT 
ACTTTGACAG ATAATTCTGC CAATACAACT ACTTCCCTTC TTAGATCCCT CTGCCATTAT 
GATGACAGTG TTCTTCTTGG TTCCTGGCTT CAAGAAACTG ATCATAAATC AATTGAAGAT 
CAGCTCCAAC CAAATAGTGC ATCTGGAAGT GGTGCTTTAG AGCATGACCC ATCCTCCATT 
TATTTACGAA TACCTGCTGG TGAAGCTGTT CCTGGTCCTC TTCCGCTTGG AGTCTCAGTC 
ATTGATGCTT CTGTTGCTCT CTTTGGTGTC GTATTTCCTC ATGTTTCTTA CAAACACCGA 
TTACAAATGC TGGATCATTT TGCTGAGTGT GTCAAACAGG CTAAAGGTGT TCGTCAACAG 
GCTGTACAGC TTAATATTTT CACTGCTGTT CTTAGTGCAT TAAAGGGCTT GGCTGAAAAC 
AAAAGTACTT TAGGACCTGA GGAAGTTCGG AAATCTGCTC TGACATTGGT TATGGGAGCT 
CTCGATAATC CAAATCCCAT TTTGAGATGT GCAGCTGGAG AAGCCCTTGG AAGAATGGCT 
CAAGTGGTCG GAGAAGCAAC TTTCATTGCT AGAATGGCTC AATATAGCTT TGACAAATTA 
AAATCTGCTC GAGATGTTGT TTCCAGAACT GGCCATTCAT TGGCTCTTGG CTGTCTACAT 
CGATATGTTG GGGGGATAGG ATCAGGACAA CACCTCAAGA CCAGTGTTAG TATTCTACTG 
GCACTAGCTC AAGATGGAAC TTCACCTGAA GTCCAGACCT GGTCTCTTCA TTCACTTGCT 
TTAATAGTGG ACTCTAGTGG GCCAATGTAT CGTGGCTATG TTGAACCAAC CTTGTCCCTA 
GTCCTTACAT TATTATTGAC AGTTCCACCT TCACATACAG AGGTTCATCA ATGTTTGGGT 
CGATGTTTAG GTGCTATAAT AACCACCGTT GGACCTGAAC TGCAGGGGAA TGGAGCAACA 
ATCTCAACAA TCCGCTCTTC CTGTTTGGTT GGTTGTGCAA TTACACAAGA CCATTCAGAT 
TCTCTTGTTC AGGCAGCTGC CATATCTTGC CTTCAACAAC TACATATGTT TGCACCACGA 
CATGTCAATC TATCTAGTCT GGTTCCCAGC CTTTGTGTTC ATTTATGTAG TTCCCACTTA 
TTACTCCGAA GGGCAGCAGT TGCATGTCTT CGACAACTTG CACAGCGAGA AGCTGCTGAA 
GTATGTGAAT ATGCTATGAG CCTTGCAAAA AATGCAGGAG ACAAAGAAAG CAGTGGCATC 
AATATGAATC CTTTTGCACC AGGGGTCAGT TCTCGCCGTG ATATCCATTG TCGACACCAA 
GGTGTCAATA TAACAGAAAC TGGTTTAGAA GGGCTTCTTT TTGGAATGCT AGACCGGGAG 
ACTGATAGAA AATTATGTTC GGATATCCAT GATACTTTAG GACATATGCT TTCATCTCTG 
GCTGTAGACA AACTTTCACA TTGGCTAATG CTTTGTAAGG ATGTTCTGGC AGCATCCAGT 
GATATGAGCA CTACAACCCC TTTAAGTAGT GGAAAGGATG AAGAATCTGA GAAGAAGGAT 
GAGATGGATG ATGATACTAT GTTTACCACA TTAGGTGAAG AGGATAAATC AAAGCCATCT 
GTAGCTCCTC GCTGGGCTAC TCGAGTGTTT GCTGCTGACT GCTTGTGTCG AGTCATCATG 
TTGTGTGAGA ATGCAGATCA GGCTCATTTT GATCTTGCTA TGGCACGTTC AGCCAAATTG 
AAAAATCCTG CAAATGATCT TCTAGTGCTC CATCTCTCTG ACCTGATACG CATGGCATTC 
ATGGCTGCTA CAGATCACAG CAACCAGCTG CGGATGGCTG GACTCCAAGC ACTTGAGGAC 
ATTATCAAGA AATTTGCATC CGTGCCTGAG CCAGAATTTC CAGGTCATGT GATTTTGGAG 
CAGTACCAAG CTAATGTGGG AGCTGCTCTC AGACCAGCTT TTTCACAAGA TACTCCATCA 
GACATAATTG CAAAAGCTTG CCAGGTTTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT TTGCTTGTTT CCTCACTTGA CAAAGTACAG 
GCAGGAAAAG GATCTTCCAG CCAACTTTAT AGAGAGAGTG CAACAACCAT GGAAAAATTG 
GCAGTGCTAA AAGCTTGGGC TGAGGTTTAT GTTGTTGCCA TGAATATTAA AAAGGAAGCA 
GAAACTAAAC CAAAAAGAGC CATAAAAAAT ACTGATGATG AAGATGATGA TTATGGGACC 
ATAGATGAGT TGCCACCAGA CAGTTTAATT ACACTTGTAC AACCTGAATT GCCAACACTT 
AGCCGCCTCT GGTTGGCAGC ATTAAAAGAT TATGCACTTT TGACTTTACC AGCTGAATTT 
TCAAGTCAGC TTCCACCAGA TGGGGGAGCA TTTTATACTC CAGAGACGAT TGATACGGCT 
CGACTTCACT ATCGGAACTC TTGGGCTCCA ATTCTCCATG CAGTAGCACT TTGGTTAAAT 
AGCACAGGAT TTACTAATTC AGAAGCAACA GAAGAAACAT CAGTATCTGG TTTGCAAAAA 
CGTCCAACAC CTGTCATTTT AAACCAAATA CCTGGAGCAA CAGCTAATGC TAAATCTTTG 
CCAGAAATCA ACAAGGACAG AATGCATCTG ATTTTAGGTG TTAGTATACA GTTTCTCTGT 
TCCCCAAGAC CTGAGGAACC TATTGAACAT GTTACAGCCT GTCTGCAGGC ATTGCATACC 
TTGCTGGAAT CCCCTTGTGC CAGAATTCAT ATTGGAGAAG ATCAGCTCAT TGGTGTTGAG 
TTGCTGAATG TGCTACATCG ACTCCTTTTG ACTTGGAATC CACCCTCAGT TCAGCTGTTG 
GTTACTGGAG TGGTACAGCA GATAGTTAGA GCAGCTCAAG ATTATTTGCA AGAAAAAAGA 
AACACTCTAA ATGAAGATGA TATGGAAAAA GAGAGTTGCC CTGTTCTAGG AGAAGGAGGT 
GACAATGGTG GTCTTGTGCC TGGAAAATCT CTTGTCTTTG CAACCATGGA GCTATTGATG 
TTCATCTTAG TACGGCACAT GCCACATCTC AGTACCAAGA TGTCAGATTC ACCAAGTCAT 
ATATCCACTA AAACTCGATT ATCTGAAGAA AGTGCTCGTT TAGTGGCAGC GGCAGTTACC 
ATCCTCTCAG ACTTGCCATC TCTTTGCTCT CCTGCTGGAT GTATGACAAT ATTACCCACA 
ATTCTCTTTC TGATTGCAAG AATATTGAAA GATACTGCAA TAAAGTCTGG TGATAATCAA 
GTACCCCCTC CAGTTAATGC AGCTCTACAA GGAATTAAAA ACATTGTGAC TCTCTCAATG 
GCCAAAACTG ATGAAACGCA AAAACAGTGG ACTGATCTAA TTCGTAGCAC TCTTGCATGT 
ATCCTGGAAT ATTCTCAACC AGAAGATTCG ATGCCAATTC CCGATGAAGT GAGCATGCTT 
ACAGCCATTG CACTCTTTCT ATGGTCTGCT AGTACTGAAA TAATTGGAGT TCAGTCATTA 
CAGAATGGTT GCATGAACAG ATTTAAAAAT GCACTGAATT CTTGTGATCC TTGGGTTCAA 
GCTAAGTGTT ACCAGCTTCT TCTTTCAGTC TTCCAGCATT CCAACCGTGC CCTTTCAACT 
CCCTACATCC ATTCATTAGC TCCAATAATG GTTGAAAAAC TAAAAGCTGT TGAAAGGAGC 
CGGCCAACCA GTAGTACAGA GCTTTTAGCT GTTCAAGAAG GAATCAAAGT TCTTGAAACA 
TTGGTTGCCC TTGGTGAAGA GCAGAACAGA GTCCAGCTAC TTGCTCTTTT AGTTCCAACT 
TTGATTTCAT ATTTGTTAGA TGAAAATGCT TTTGATTCAG CACTTACGGC ATCCAAAGAT 
CTTCATGAAT TTGCACTCCA GAATTTAATG CACATTGGGC CTCTCTATCC ACATGCTTTC 
AAGACTGTTA TGGGAGCTGC TCCTGAATTG AAAACAAGAC TCGAAACTGC AGTTCGCGCA 
AGTCAAGCTA GTAAAGCAAA AGCAGCTGCC AGGCAACCAG CACCTACCAT ACATTCCGCT 
CCAACAATTA AACTCAAAAC AAGCTTTTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031807130.1
CDS	173..6385
Translation	MELAHSLLLNEEALAQITEAKRPVFIFEWLRFLDKVLVAANKTDVKEKQKRLVEQLTGLISSSPGPPTRKLLAKNLAALYSIGDTFTVFQTLDKCNDIIKNKDDTAAYLPTKLAAVACVGAFYEKMGRMLGSAFPETVNNLLKSLKNAESQGRSEILMSLQKVLNGLGGAAASCHRDIYKNARSLLSDRSMAVRCAVAKCLLELQNEAVFMWTAELENIATLSFKALESSNYGVRVAVAKLLGTVMATALMPKQAMVMRQNVKRATLDEVLELMATGFLRGGSGFLKSGGEMLKVGGSVNREVRVGVTQAYVVFVTTLGGQWLERSFATFLSHVLDLVSHPRATQTHVEAVYSRRCVSFILRATVGSLLGEKAQIAAAKEICQAIGKQMKAVEAVVNDTSSENKSGAADIAASQHVMVCALQELGSLVQSLNATASPLIQEASIGLLETVTSVLLHPSMAARLAAAWCLRCVAVALPFQLTPFLDRCAERLNNLKTSPEAVSGYSFAMAALLGGVHQCPLGIPHAKGKMVVSIAEDLLRTAAQNSRLSLQRTQAGWLLLGALMTLGPSVVRYHLPKMLLLWRNVFPRSLKELEAEKARGDSFTWQVTLEGRAGALCAMRSFVAHCPELLTEDVIRKLMTPIECAMTMMSHIPSVIKAHGAHLKASAAMVRLRLYDILALLPPKTYEGSFNALLRELVAEFTLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAVPGPLPLGVSVIDASVALFGVVFPHVSYKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAENKSTLGPEEVRKSALTLVMGALDNPNPILRCAAGEALGRMAQVVGEATFIARMAQYSFDKLKSARDVVSRTGHSLALGCLHRYVGGIGSGQHLKTSVSILLALAQDGTSPEVQTWSLHSLALIVDSSGPMYRGYVEPTLSLVLTLLLTVPPSHTEVHQCLGRCLGAIITTVGPELQGNGATISTIRSSCLVGCAITQDHSDSLVQAAAISCLQQLHMFAPRHVNLSSLVPSLCVHLCSSHLLLRRAAVACLRQLAQREAAEVCEYAMSLAKNAGDKESSGINMNPFAPGVSSRRDIHCRHQGVNITETGLEGLLFGMLDRETDRKLCSDIHDTLGHMLSSLAVDKLSHWLMLCKDVLAASSDMSTTTPLSSGKDEESEKKDEMDDDTMFTTLGEEDKSKPSVAPRWATRVFAADCLCRVIMLCENADQAHFDLAMARSAKLKNPANDLLVLHLSDLIRMAFMAATDHSNQLRMAGLQALEDIIKKFASVPEPEFPGHVILEQYQANVGAALRPAFSQDTPSDIIAKACQVCSTWIGSGVVSDLNDLRRVHNLLVSSLDKVQAGKGSSSQLYRESATTMEKLAVLKAWAEVYVVAMNIKKEAETKPKRAIKNTDDEDDDYGTIDELPPDSLITLVQPELPTLSRLWLAALKDYALLTLPAEFSSQLPPDGGAFYTPETIDTARLHYRNSWAPILHAVALWLNSTGFTNSEATEETSVSGLQKRPTPVILNQIPGATANAKSLPEINKDRMHLILGVSIQFLCSPRPEEPIEHVTACLQALHTLLESPCARIHIGEDQLIGVELLNVLHRLLLTWNPPSVQLLVTGVVQQIVRAAQDYLQEKRNTLNEDDMEKESCPVLGEGGDNGGLVPGKSLVFATMELLMFILVRHMPHLSTKMSDSPSHISTKTRLSEESARLVAAAVTILSDLPSLCSPAGCMTILPTILFLIARILKDTAIKSGDNQVPPPVNAALQGIKNIVTLSMAKTDETQKQWTDLIRSTLACILEYSQPEDSMPIPDEVSMLTAIALFLWSASTEIIGVQSLQNGCMNRFKNALNSCDPWVQAKCYQLLLSVFQHSNRALSTPYIHSLAPIMVEKLKAVERSRPTSSTELLAVQEGIKVLETLVALGEEQNRVQLLALLVPTLISYLLDENAFDSALTASKDLHEFALQNLMHIGPLYPHAFKTVMGAAPELKTRLETAVRASQASKAKAAARQPAPTIHSAPTIKLKTSFF

Target ORF information:

RefSeq Version	XM_031951270.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HEAT repeat containing 5B (HEATR5B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031951270.1

ORF Insert Sequence:

ATGGAGTTGG CCCATAGTTT ATTGCTAAAT GAAGAAGCTT TGGCCCAAAT TACAGAAGCA 
AAGAGGCCTG TTTTTATCTT TGAGTGGTTG CGATTTCTTG ACAAAGTCCT GGTAGCTGCT 
AACAAGACTG ATGTGAAGGA AAAACAGAAA AGGCTTGTTG AACAACTCAC TGGATTAATA 
AGTAGTTCTC CTGGCCCCCC AACAAGGAAA TTACTAGCTA AAAATCTTGC AGCTCTTTAT 
AGCATTGGAG ATACTTTCAC TGTTTTTCAA ACTCTTGACA AGTGTAATGA TATTATCAAA 
AACAAAGATG ATACTGCAGC CTATTTGCCA ACCAAACTGG CTGCAGTAGC ATGTGTTGGG 
GCATTTTATG AAAAAATGGG GAGGATGTTA GGAAGTGCAT TTCCAGAAAC AGTTAACAAT 
CTTTTGAAGT CTCTGAAAAA TGCAGAGTCT CAAGGCCGAA GTGAAATCTT AATGAGTTTA 
CAAAAAGTCC TAAATGGACT GGGTGGTGCA GCAGCTTCTT GTCATCGTGA TATTTATAAG 
AATGCAAGAT CTCTGCTAAG TGACAGATCT ATGGCTGTTA GGTGTGCAGT TGCTAAATGT 
CTGCTAGAAC TACAAAATGA AGCTGTATTT ATGTGGACAG CTGAATTAGA AAATATAGCC 
ACATTATCTT TTAAGGCTTT GGAAAGCTCA AATTATGGGG TTCGAGTGGC AGTGGCAAAA 
CTCTTGGGAA CAGTCATGGC AACTGCATTG ATGCCTAAAC AGGCAATGGT AATGCGCCAG 
AATGTGAAAA GAGCCACTTT AGATGAAGTC TTAGAGCTCA TGGCCACAGG ATTTCTGCGA 
GGAGGGTCTG GATTCTTAAA GAGTGGTGGA GAAATGCTAA AAGTTGGTGG GTCAGTTAAT 
CGTGAAGTAA GAGTTGGAGT TACACAGGCA TATGTTGTTT TTGTTACAAC ATTAGGTGGT 
CAGTGGTTAG AACGCAGCTT TGCTACATTC TTATCCCATG TACTTGATCT GGTTTCACAC 
CCTCGGGCCA CTCAAACACA TGTAGAAGCT GTGTATTCAA GAAGATGTGT CTCCTTTATC 
CTCAGAGCTA CTGTAGGTAG TTTGCTTGGG GAAAAAGCTC AGATTGCAGC TGCCAAAGAA 
ATCTGCCAAG CCATTGGAAA ACAAATGAAA GCAGTGGAAG CAGTTGTCAA TGATACAAGT 
AGTGAAAACA AATCTGGAGC TGCTGACATA GCAGCAAGTC AGCATGTGAT GGTGTGTGCT 
CTGCAGGAAC TTGGAAGCTT AGTCCAAAGC TTAAATGCCA CTGCATCTCC TCTTATTCAG 
GAAGCATCTA TAGGTCTTTT GGAAACTGTA ACATCAGTGC TTCTGCATCC TAGTATGGCT 
GCCCGACTTG CTGCAGCATG GTGTTTGCGT TGTGTAGCTG TGGCCTTGCC TTTCCAATTG 
ACCCCATTTC TAGACAGATG TGCAGAAAGG CTCAATAATT TGAAGACCTC CCCTGAAGCT 
GTTAGTGGTT ACAGTTTTGC AATGGCTGCT TTATTAGGTG GAGTACATCA GTGCCCTTTG 
GGTATCCCTC ATGCCAAAGG AAAGATGGTA GTAAGCATTG CTGAAGATCT CTTACGAACT 
GCTGCTCAGA ACAGTAGGCT ATCCTTGCAA CGTACTCAAG CTGGATGGCT TCTACTTGGA 
GCACTTATGA CTTTAGGTCC TTCTGTTGTT CGTTACCATC TTCCCAAAAT GCTGTTATTA 
TGGCGAAATG TCTTCCCTCG TTCCTTAAAG GAACTAGAAG CTGAAAAGGC ACGAGGAGAT 
TCTTTTACGT GGCAGGTAAC ATTGGAAGGC CGAGCTGGAG CTTTATGTGC CATGAGGAGT 
TTTGTTGCTC ATTGTCCTGA GCTTTTAACT GAGGATGTGA TTAGAAAATT GATGACGCCT 
ATTGAATGTG CCATGACAAT GATGTCACAC ATTCCTTCTG TAATTAAAGC CCATGGAGCT 
CATTTGAAGG CCAGTGCTGC AATGGTTCGT TTAAGACTTT ACGATATTTT GGCATTGTTA 
CCCCCCAAAA CTTATGAAGG ATCTTTTAAT GCACTTCTTA GGGAGCTGGT GGCAGAATTT 
ACTTTGACAG ATAATTCTGC CAATACAACT ACTTCCCTTC TTAGATCCCT CTGCCATTAT 
GATGACAGTG TTCTTCTTGG TTCCTGGCTT CAAGAAACTG ATCATAAATC AATTGAAGAT 
CAGCTCCAAC CAAATAGTGC ATCTGGAAGT GGTGCTTTAG AGCATGACCC ATCCTCCATT 
TATTTACGAA TACCTGCTGG TGAAGCTGTT CCTGGTCCTC TTCCGCTTGG AGTCTCAGTC 
ATTGATGCTT CTGTTGCTCT CTTTGGTGTC GTATTTCCTC ATGTTTCTTA CAAACACCGA 
TTACAAATGC TGGATCATTT TGCTGAGTGT GTCAAACAGG CTAAAGGTGT TCGTCAACAG 
GCTGTACAGC TTAATATTTT CACTGCTGTT CTTAGTGCAT TAAAGGGCTT GGCTGAAAAC 
AAAAGTACTT TAGGACCTGA GGAAGTTCGG AAATCTGCTC TGACATTGGT TATGGGAGCT 
CTCGATAATC CAAATCCCAT TTTGAGATGT GCAGCTGGAG AAGCCCTTGG AAGAATGGCT 
CAAGTGGTCG GAGAAGCAAC TTTCATTGCT AGAATGGCTC AATATAGCTT TGACAAATTA 
AAATCTGCTC GAGATGTTGT TTCCAGAACT GGCCATTCAT TGGCTCTTGG CTGTCTACAT 
CGATATGTTG GGGGGATAGG ATCAGGACAA CACCTCAAGA CCAGTGTTAG TATTCTACTG 
GCACTAGCTC AAGATGGAAC TTCACCTGAA GTCCAGACCT GGTCTCTTCA TTCACTTGCT 
TTAATAGTGG ACTCTAGTGG GCCAATGTAT CGTGGCTATG TTGAACCAAC CTTGTCCCTA 
GTCCTTACAT TATTATTGAC AGTTCCACCT TCACATACAG AGGTTCATCA ATGTTTGGGT 
CGATGTTTAG GTGCTATAAT AACCACCGTT GGACCTGAAC TGCAGGGGAA TGGAGCAACA 
ATCTCAACAA TCCGCTCTTC CTGTTTGGTT GGTTGTGCAA TTACACAAGA CCATTCAGAT 
TCTCTTGTTC AGGCAGCTGC CATATCTTGC CTTCAACAAC TACATATGTT TGCACCACGA 
CATGTCAATC TATCTAGTCT GGTTCCCAGC CTTTGTGTTC ATTTATGTAG TTCCCACTTA 
TTACTCCGAA GGGCAGCAGT TGCATGTCTT CGACAACTTG CACAGCGAGA AGCTGCTGAA 
GTATGTGAAT ATGCTATGAG CCTTGCAAAA AATGCAGGAG ACAAAGAAAG CAGTGGCATC 
AATATGAATC CTTTTGCACC AGGGGTCAGT TCTCGCCGTG ATATCCATTG TCGACACCAA 
GGTGTCAATA TAACAGAAAC TGGTTTAGAA GGGCTTCTTT TTGGAATGCT AGACCGGGAG 
ACTGATAGAA AATTATGTTC GGATATCCAT GATACTTTAG GACATATGCT TTCATCTCTG 
GCTGTAGACA AACTTTCACA TTGGCTAATG CTTTGTAAGG ATGTTCTGGC AGCATCCAGT 
GATATGAGCA CTACAACCCC TTTAAGTAGT GGAAAGGATG AAGAATCTGA GAAGAAGGAT 
GAGATGGATG ATGATACTAT GTTTACCACA TTAGGTGAAG AGGATAAATC AAAGCCATCT 
GTAGCTCCTC GCTGGGCTAC TCGAGTGTTT GCTGCTGACT GCTTGTGTCG AGTCATCATG 
TTGTGTGAGA ATGCAGATCA GGCTCATTTT GATCTTGCTA TGGCACGTTC AGCCAAATTG 
AAAAATCCTG CAAATGATCT TCTAGTGCTC CATCTCTCTG ACCTGATACG CATGGCATTC 
ATGGCTGCTA CAGATCACAG CAACCAGCTG CGGATGGCTG GACTCCAAGC ACTTGAGGAC 
ATTATCAAGA AATTTGCATC CGTGCCTGAG CCAGAATTTC CAGGTCATGT GATTTTGGAG 
CAGTACCAAG CTAATGTGGG AGCTGCTCTC AGACCAGCTT TTTCACAAGA TACTCCATCA 
GACATAATTG CAAAAGCTTG CCAGGTTTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT TTGCTTGTTT CCTCACTTGA CAAAGTACAG 
GCAGGAAAAG GATCTTCCAG CCAACTTTAT AGAGAGAGTG CAACAACCAT GGAAAAATTG 
GCAGTGCTAA AAGCTTGGGC TGAGGTTTAT GTTGTTGCCA TGAATATTAA AAAGGAAGCA 
GAAACTAAAC CAAAAAGAGC CATAAAAAAT ACTGATGATG AAGATGATGA TTATGGGACC 
ATAGATGAGT TGCCACCAGA CAGTTTAATT ACACTTGTAC AACCTGAATT GCCAACACTT 
AGCCGCCTCT GGTTGGCAGC ATTAAAAGAT TATGCACTTT TGACTTTACC AGCTGAATTT 
TCAAGTCAGC TTCCACCAGA TGGGGGAGCA TTTTATACTC CAGAGACGAT TGATACGGCT 
CGACTTCACT ATCGGAACTC TTGGGCTCCA ATTCTCCATG CAGTAGCACT TTGGTTAAAT 
AGCACAGGAT TTACTAATTC AGAAGCAACA GAAGAAACAT CAGTATCTGG TTTGCAAAAA 
CGTCCAACAC CTGTCATTTT AAACCAAATA CCTGGAGCAA CAGCTAATGC TAAATCTTTG 
CCAGAAATCA ACAAGGACAG AATGCATCTG ATTTTAGGTG TTAGTATACA GTTTCTCTGT 
TCCCCAAGAC CTGAGGAACC TATTGAACAT GTTACAGCCT GTCTGCAGGC ATTGCATACC 
TTGCTGGAAT CCCCTTGTGC CAGAATTCAT ATTGGAGAAG ATCAGCTCAT TGGTGTTGAG 
TTGCTGAATG TGCTACATCG ACTCCTTTTG ACTTGGAATC CACCCTCAGT TCAGCTGTTG 
GTTACTGGAG TGGTACAGCA GATAGTTAGA GCAGCTCAAG ATTATTTGCA AGAAAAAAGA 
AACACTCTAA ATGAAGATGA TATGGAAAAA GAGAGTTGCC CTGTTCTAGG AGAAGGAGGT 
GACAATGGTG GTCTTGTGCC TGGAAAATCT CTTGTCTTTG CAACCATGGA GCTATTGATG 
TTCATCTTAG TACGGCACAT GCCACATCTC AGTACCAAGA TGTCAGATTC ACCAAGTCAT 
ATATCCACTA AAACTCGATT ATCTGAAGAA AGTGCTCGTT TAGTGGCAGC GGCAGTTACC 
ATCCTCTCAG ACTTGCCATC TCTTTGCTCT CCTGCTGGAT GTATGACAAT ATTACCCACA 
ATTCTCTTTC TGATTGCAAG AATATTGAAA GATACTGCAA TAAAGTCTGG TGATAATCAA 
GTACCCCCTC CAGTTAATGC AGCTCTACAA GGAATTAAAA ACATTGTGAC TCTCTCAATG 
GCCAAAACTG ATGAAACGCA AAAACAGTGG ACTGATCTAA TTCGTAGCAC TCTTGCATGT 
ATCCTGGAAT ATTCTCAACC AGAAGATTCG ATGCCAATTC CCGATGAAGT GAGCATGCTT 
ACAGCCATTG CACTCTTTCT ATGGTCTGCT AGTACTGAAA TAATTGGAGT TCAGTCATTA 
CAGAATGGTT GCATGAACAG ATTTAAAAAT GCACTGAATT CTTGTGATCC TTGGGTTCAA 
GCTAAGTGTT ACCAGCTTCT TCTTTCAGTC TTCCAGCATT CCAACCGTGC CCTTTCAACT 
CCCTACATCC ATTCATTAGC TCCAATAATG GTTGAAAAAC TAAAAGCTGT TGAAAGGAGC 
CGGCCAACCA GTAGTACAGA GCTTTTAGCT GTTCAAGAAG GAATCAAAGT TCTTGAAACA 
TTGGTTGCCC TTGGTGAAGA GCAGAACAGA GTCCAGCTAC TTGCTCTTTT AGTTCCAACT 
TTGATTTCAT ATTTGTTAGA TGAAAATGCT TTTGATTCAG CACTTACGGC ATCCAAAGAT 
CTTCATGAAT TTGCACTCCA GAATTTAATG CACATTGGGC CTCTCTATCC ACATGCTTTC 
AAGACTGTTA TGGGAGCTGC TCCTGAATTG AAAACAAGAC TCGAAACTGC AGTTCGCGCA 
AGTCAAGCTA GTAAAGCAAA AGCAGCTGCC AGGCAACCAG CACCTACCAT ACATTCCGCT 
CCAACAATTA AACTCAAAAC AAGCTTTTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5B ( XM_031951272.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031807132.1 Sarcophilus harrisii HEAT repeat containing 5B (HEATR5B), transcript variant X3, mRNA.

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CloneID	OSa224487
Clone ID Related Accession (Same CDS sequence)	XM_031951270.1 , XM_031951272.1
Accession Version	XM_031951272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	HEAT repeat-containing protein 5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGG CCCATAGTTT ATTGCTAAAT GAAGAAGCTT TGGCCCAAAT TACAGAAGCA 
AAGAGGCCTG TTTTTATCTT TGAGTGGTTG CGATTTCTTG ACAAAGTCCT GGTAGCTGCT 
AACAAGACTG ATGTGAAGGA AAAACAGAAA AGGCTTGTTG AACAACTCAC TGGATTAATA 
AGTAGTTCTC CTGGCCCCCC AACAAGGAAA TTACTAGCTA AAAATCTTGC AGCTCTTTAT 
AGCATTGGAG ATACTTTCAC TGTTTTTCAA ACTCTTGACA AGTGTAATGA TATTATCAAA 
AACAAAGATG ATACTGCAGC CTATTTGCCA ACCAAACTGG CTGCAGTAGC ATGTGTTGGG 
GCATTTTATG AAAAAATGGG GAGGATGTTA GGAAGTGCAT TTCCAGAAAC AGTTAACAAT 
CTTTTGAAGT CTCTGAAAAA TGCAGAGTCT CAAGGCCGAA GTGAAATCTT AATGAGTTTA 
CAAAAAGTCC TAAATGGACT GGGTGGTGCA GCAGCTTCTT GTCATCGTGA TATTTATAAG 
AATGCAAGAT CTCTGCTAAG TGACAGATCT ATGGCTGTTA GGTGTGCAGT TGCTAAATGT 
CTGCTAGAAC TACAAAATGA AGCTGTATTT ATGTGGACAG CTGAATTAGA AAATATAGCC 
ACATTATCTT TTAAGGCTTT GGAAAGCTCA AATTATGGGG TTCGAGTGGC AGTGGCAAAA 
CTCTTGGGAA CAGTCATGGC AACTGCATTG ATGCCTAAAC AGGCAATGGT AATGCGCCAG 
AATGTGAAAA GAGCCACTTT AGATGAAGTC TTAGAGCTCA TGGCCACAGG ATTTCTGCGA 
GGAGGGTCTG GATTCTTAAA GAGTGGTGGA GAAATGCTAA AAGTTGGTGG GTCAGTTAAT 
CGTGAAGTAA GAGTTGGAGT TACACAGGCA TATGTTGTTT TTGTTACAAC ATTAGGTGGT 
CAGTGGTTAG AACGCAGCTT TGCTACATTC TTATCCCATG TACTTGATCT GGTTTCACAC 
CCTCGGGCCA CTCAAACACA TGTAGAAGCT GTGTATTCAA GAAGATGTGT CTCCTTTATC 
CTCAGAGCTA CTGTAGGTAG TTTGCTTGGG GAAAAAGCTC AGATTGCAGC TGCCAAAGAA 
ATCTGCCAAG CCATTGGAAA ACAAATGAAA GCAGTGGAAG CAGTTGTCAA TGATACAAGT 
AGTGAAAACA AATCTGGAGC TGCTGACATA GCAGCAAGTC AGCATGTGAT GGTGTGTGCT 
CTGCAGGAAC TTGGAAGCTT AGTCCAAAGC TTAAATGCCA CTGCATCTCC TCTTATTCAG 
GAAGCATCTA TAGGTCTTTT GGAAACTGTA ACATCAGTGC TTCTGCATCC TAGTATGGCT 
GCCCGACTTG CTGCAGCATG GTGTTTGCGT TGTGTAGCTG TGGCCTTGCC TTTCCAATTG 
ACCCCATTTC TAGACAGATG TGCAGAAAGG CTCAATAATT TGAAGACCTC CCCTGAAGCT 
GTTAGTGGTT ACAGTTTTGC AATGGCTGCT TTATTAGGTG GAGTACATCA GTGCCCTTTG 
GGTATCCCTC ATGCCAAAGG AAAGATGGTA GTAAGCATTG CTGAAGATCT CTTACGAACT 
GCTGCTCAGA ACAGTAGGCT ATCCTTGCAA CGTACTCAAG CTGGATGGCT TCTACTTGGA 
GCACTTATGA CTTTAGGTCC TTCTGTTGTT CGTTACCATC TTCCCAAAAT GCTGTTATTA 
TGGCGAAATG TCTTCCCTCG TTCCTTAAAG GAACTAGAAG CTGAAAAGGC ACGAGGAGAT 
TCTTTTACGT GGCAGGTAAC ATTGGAAGGC CGAGCTGGAG CTTTATGTGC CATGAGGAGT 
TTTGTTGCTC ATTGTCCTGA GCTTTTAACT GAGGATGTGA TTAGAAAATT GATGACGCCT 
ATTGAATGTG CCATGACAAT GATGTCACAC ATTCCTTCTG TAATTAAAGC CCATGGAGCT 
CATTTGAAGG CCAGTGCTGC AATGGTTCGT TTAAGACTTT ACGATATTTT GGCATTGTTA 
CCCCCCAAAA CTTATGAAGG ATCTTTTAAT GCACTTCTTA GGGAGCTGGT GGCAGAATTT 
ACTTTGACAG ATAATTCTGC CAATACAACT ACTTCCCTTC TTAGATCCCT CTGCCATTAT 
GATGACAGTG TTCTTCTTGG TTCCTGGCTT CAAGAAACTG ATCATAAATC AATTGAAGAT 
CAGCTCCAAC CAAATAGTGC ATCTGGAAGT GGTGCTTTAG AGCATGACCC ATCCTCCATT 
TATTTACGAA TACCTGCTGG TGAAGCTGTT CCTGGTCCTC TTCCGCTTGG AGTCTCAGTC 
ATTGATGCTT CTGTTGCTCT CTTTGGTGTC GTATTTCCTC ATGTTTCTTA CAAACACCGA 
TTACAAATGC TGGATCATTT TGCTGAGTGT GTCAAACAGG CTAAAGGTGT TCGTCAACAG 
GCTGTACAGC TTAATATTTT CACTGCTGTT CTTAGTGCAT TAAAGGGCTT GGCTGAAAAC 
AAAAGTACTT TAGGACCTGA GGAAGTTCGG AAATCTGCTC TGACATTGGT TATGGGAGCT 
CTCGATAATC CAAATCCCAT TTTGAGATGT GCAGCTGGAG AAGCCCTTGG AAGAATGGCT 
CAAGTGGTCG GAGAAGCAAC TTTCATTGCT AGAATGGCTC AATATAGCTT TGACAAATTA 
AAATCTGCTC GAGATGTTGT TTCCAGAACT GGCCATTCAT TGGCTCTTGG CTGTCTACAT 
CGATATGTTG GGGGGATAGG ATCAGGACAA CACCTCAAGA CCAGTGTTAG TATTCTACTG 
GCACTAGCTC AAGATGGAAC TTCACCTGAA GTCCAGACCT GGTCTCTTCA TTCACTTGCT 
TTAATAGTGG ACTCTAGTGG GCCAATGTAT CGTGGCTATG TTGAACCAAC CTTGTCCCTA 
GTCCTTACAT TATTATTGAC AGTTCCACCT TCACATACAG AGGTTCATCA ATGTTTGGGT 
CGATGTTTAG GTGCTATAAT AACCACCGTT GGACCTGAAC TGCAGGGGAA TGGAGCAACA 
ATCTCAACAA TCCGCTCTTC CTGTTTGGTT GGTTGTGCAA TTACACAAGA CCATTCAGAT 
TCTCTTGTTC AGGCAGCTGC CATATCTTGC CTTCAACAAC TACATATGTT TGCACCACGA 
CATGTCAATC TATCTAGTCT GGTTCCCAGC CTTTGTGTTC ATTTATGTAG TTCCCACTTA 
TTACTCCGAA GGGCAGCAGT TGCATGTCTT CGACAACTTG CACAGCGAGA AGCTGCTGAA 
GTATGTGAAT ATGCTATGAG CCTTGCAAAA AATGCAGGAG ACAAAGAAAG CAGTGGCATC 
AATATGAATC CTTTTGCACC AGGGGTCAGT TCTCGCCGTG ATATCCATTG TCGACACCAA 
GGTGTCAATA TAACAGAAAC TGGTTTAGAA GGGCTTCTTT TTGGAATGCT AGACCGGGAG 
ACTGATAGAA AATTATGTTC GGATATCCAT GATACTTTAG GACATATGCT TTCATCTCTG 
GCTGTAGACA AACTTTCACA TTGGCTAATG CTTTGTAAGG ATGTTCTGGC AGCATCCAGT 
GATATGAGCA CTACAACCCC TTTAAGTAGT GGAAAGGATG AAGAATCTGA GAAGAAGGAT 
GAGATGGATG ATGATACTAT GTTTACCACA TTAGGTGAAG AGGATAAATC AAAGCCATCT 
GTAGCTCCTC GCTGGGCTAC TCGAGTGTTT GCTGCTGACT GCTTGTGTCG AGTCATCATG 
TTGTGTGAGA ATGCAGATCA GGCTCATTTT GATCTTGCTA TGGCACGTTC AGCCAAATTG 
AAAAATCCTG CAAATGATCT TCTAGTGCTC CATCTCTCTG ACCTGATACG CATGGCATTC 
ATGGCTGCTA CAGATCACAG CAACCAGCTG CGGATGGCTG GACTCCAAGC ACTTGAGGAC 
ATTATCAAGA AATTTGCATC CGTGCCTGAG CCAGAATTTC CAGGTCATGT GATTTTGGAG 
CAGTACCAAG CTAATGTGGG AGCTGCTCTC AGACCAGCTT TTTCACAAGA TACTCCATCA 
GACATAATTG CAAAAGCTTG CCAGGTTTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT TTGCTTGTTT CCTCACTTGA CAAAGTACAG 
GCAGGAAAAG GATCTTCCAG CCAACTTTAT AGAGAGAGTG CAACAACCAT GGAAAAATTG 
GCAGTGCTAA AAGCTTGGGC TGAGGTTTAT GTTGTTGCCA TGAATATTAA AAAGGAAGCA 
GAAACTAAAC CAAAAAGAGC CATAAAAAAT ACTGATGATG AAGATGATGA TTATGGGACC 
ATAGATGAGT TGCCACCAGA CAGTTTAATT ACACTTGTAC AACCTGAATT GCCAACACTT 
AGCCGCCTCT GGTTGGCAGC ATTAAAAGAT TATGCACTTT TGACTTTACC AGCTGAATTT 
TCAAGTCAGC TTCCACCAGA TGGGGGAGCA TTTTATACTC CAGAGACGAT TGATACGGCT 
CGACTTCACT ATCGGAACTC TTGGGCTCCA ATTCTCCATG CAGTAGCACT TTGGTTAAAT 
AGCACAGGAT TTACTAATTC AGAAGCAACA GAAGAAACAT CAGTATCTGG TTTGCAAAAA 
CGTCCAACAC CTGTCATTTT AAACCAAATA CCTGGAGCAA CAGCTAATGC TAAATCTTTG 
CCAGAAATCA ACAAGGACAG AATGCATCTG ATTTTAGGTG TTAGTATACA GTTTCTCTGT 
TCCCCAAGAC CTGAGGAACC TATTGAACAT GTTACAGCCT GTCTGCAGGC ATTGCATACC 
TTGCTGGAAT CCCCTTGTGC CAGAATTCAT ATTGGAGAAG ATCAGCTCAT TGGTGTTGAG 
TTGCTGAATG TGCTACATCG ACTCCTTTTG ACTTGGAATC CACCCTCAGT TCAGCTGTTG 
GTTACTGGAG TGGTACAGCA GATAGTTAGA GCAGCTCAAG ATTATTTGCA AGAAAAAAGA 
AACACTCTAA ATGAAGATGA TATGGAAAAA GAGAGTTGCC CTGTTCTAGG AGAAGGAGGT 
GACAATGGTG GTCTTGTGCC TGGAAAATCT CTTGTCTTTG CAACCATGGA GCTATTGATG 
TTCATCTTAG TACGGCACAT GCCACATCTC AGTACCAAGA TGTCAGATTC ACCAAGTCAT 
ATATCCACTA AAACTCGATT ATCTGAAGAA AGTGCTCGTT TAGTGGCAGC GGCAGTTACC 
ATCCTCTCAG ACTTGCCATC TCTTTGCTCT CCTGCTGGAT GTATGACAAT ATTACCCACA 
ATTCTCTTTC TGATTGCAAG AATATTGAAA GATACTGCAA TAAAGTCTGG TGATAATCAA 
GTACCCCCTC CAGTTAATGC AGCTCTACAA GGAATTAAAA ACATTGTGAC TCTCTCAATG 
GCCAAAACTG ATGAAACGCA AAAACAGTGG ACTGATCTAA TTCGTAGCAC TCTTGCATGT 
ATCCTGGAAT ATTCTCAACC AGAAGATTCG ATGCCAATTC CCGATGAAGT GAGCATGCTT 
ACAGCCATTG CACTCTTTCT ATGGTCTGCT AGTACTGAAA TAATTGGAGT TCAGTCATTA 
CAGAATGGTT GCATGAACAG ATTTAAAAAT GCACTGAATT CTTGTGATCC TTGGGTTCAA 
GCTAAGTGTT ACCAGCTTCT TCTTTCAGTC TTCCAGCATT CCAACCGTGC CCTTTCAACT 
CCCTACATCC ATTCATTAGC TCCAATAATG GTTGAAAAAC TAAAAGCTGT TGAAAGGAGC 
CGGCCAACCA GTAGTACAGA GCTTTTAGCT GTTCAAGAAG GAATCAAAGT TCTTGAAACA 
TTGGTTGCCC TTGGTGAAGA GCAGAACAGA GTCCAGCTAC TTGCTCTTTT AGTTCCAACT 
TTGATTTCAT ATTTGTTAGA TGAAAATGCT TTTGATTCAG CACTTACGGC ATCCAAAGAT 
CTTCATGAAT TTGCACTCCA GAATTTAATG CACATTGGGC CTCTCTATCC ACATGCTTTC 
AAGACTGTTA TGGGAGCTGC TCCTGAATTG AAAACAAGAC TCGAAACTGC AGTTCGCGCA 
AGTCAAGCTA GTAAAGCAAA AGCAGCTGCC AGGCAACCAG CACCTACCAT ACATTCCGCT 
CCAACAATTA AACTCAAAAC AAGCTTTTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031807132.1
CDS	492..6704
Translation	MELAHSLLLNEEALAQITEAKRPVFIFEWLRFLDKVLVAANKTDVKEKQKRLVEQLTGLISSSPGPPTRKLLAKNLAALYSIGDTFTVFQTLDKCNDIIKNKDDTAAYLPTKLAAVACVGAFYEKMGRMLGSAFPETVNNLLKSLKNAESQGRSEILMSLQKVLNGLGGAAASCHRDIYKNARSLLSDRSMAVRCAVAKCLLELQNEAVFMWTAELENIATLSFKALESSNYGVRVAVAKLLGTVMATALMPKQAMVMRQNVKRATLDEVLELMATGFLRGGSGFLKSGGEMLKVGGSVNREVRVGVTQAYVVFVTTLGGQWLERSFATFLSHVLDLVSHPRATQTHVEAVYSRRCVSFILRATVGSLLGEKAQIAAAKEICQAIGKQMKAVEAVVNDTSSENKSGAADIAASQHVMVCALQELGSLVQSLNATASPLIQEASIGLLETVTSVLLHPSMAARLAAAWCLRCVAVALPFQLTPFLDRCAERLNNLKTSPEAVSGYSFAMAALLGGVHQCPLGIPHAKGKMVVSIAEDLLRTAAQNSRLSLQRTQAGWLLLGALMTLGPSVVRYHLPKMLLLWRNVFPRSLKELEAEKARGDSFTWQVTLEGRAGALCAMRSFVAHCPELLTEDVIRKLMTPIECAMTMMSHIPSVIKAHGAHLKASAAMVRLRLYDILALLPPKTYEGSFNALLRELVAEFTLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAVPGPLPLGVSVIDASVALFGVVFPHVSYKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAENKSTLGPEEVRKSALTLVMGALDNPNPILRCAAGEALGRMAQVVGEATFIARMAQYSFDKLKSARDVVSRTGHSLALGCLHRYVGGIGSGQHLKTSVSILLALAQDGTSPEVQTWSLHSLALIVDSSGPMYRGYVEPTLSLVLTLLLTVPPSHTEVHQCLGRCLGAIITTVGPELQGNGATISTIRSSCLVGCAITQDHSDSLVQAAAISCLQQLHMFAPRHVNLSSLVPSLCVHLCSSHLLLRRAAVACLRQLAQREAAEVCEYAMSLAKNAGDKESSGINMNPFAPGVSSRRDIHCRHQGVNITETGLEGLLFGMLDRETDRKLCSDIHDTLGHMLSSLAVDKLSHWLMLCKDVLAASSDMSTTTPLSSGKDEESEKKDEMDDDTMFTTLGEEDKSKPSVAPRWATRVFAADCLCRVIMLCENADQAHFDLAMARSAKLKNPANDLLVLHLSDLIRMAFMAATDHSNQLRMAGLQALEDIIKKFASVPEPEFPGHVILEQYQANVGAALRPAFSQDTPSDIIAKACQVCSTWIGSGVVSDLNDLRRVHNLLVSSLDKVQAGKGSSSQLYRESATTMEKLAVLKAWAEVYVVAMNIKKEAETKPKRAIKNTDDEDDDYGTIDELPPDSLITLVQPELPTLSRLWLAALKDYALLTLPAEFSSQLPPDGGAFYTPETIDTARLHYRNSWAPILHAVALWLNSTGFTNSEATEETSVSGLQKRPTPVILNQIPGATANAKSLPEINKDRMHLILGVSIQFLCSPRPEEPIEHVTACLQALHTLLESPCARIHIGEDQLIGVELLNVLHRLLLTWNPPSVQLLVTGVVQQIVRAAQDYLQEKRNTLNEDDMEKESCPVLGEGGDNGGLVPGKSLVFATMELLMFILVRHMPHLSTKMSDSPSHISTKTRLSEESARLVAAAVTILSDLPSLCSPAGCMTILPTILFLIARILKDTAIKSGDNQVPPPVNAALQGIKNIVTLSMAKTDETQKQWTDLIRSTLACILEYSQPEDSMPIPDEVSMLTAIALFLWSASTEIIGVQSLQNGCMNRFKNALNSCDPWVQAKCYQLLLSVFQHSNRALSTPYIHSLAPIMVEKLKAVERSRPTSSTELLAVQEGIKVLETLVALGEEQNRVQLLALLVPTLISYLLDENAFDSALTASKDLHEFALQNLMHIGPLYPHAFKTVMGAAPELKTRLETAVRASQASKAKAAARQPAPTIHSAPTIKLKTSFF

Target ORF information:

RefSeq Version	XM_031951272.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HEAT repeat containing 5B (HEATR5B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031951272.1

ORF Insert Sequence:

ATGGAGTTGG CCCATAGTTT ATTGCTAAAT GAAGAAGCTT TGGCCCAAAT TACAGAAGCA 
AAGAGGCCTG TTTTTATCTT TGAGTGGTTG CGATTTCTTG ACAAAGTCCT GGTAGCTGCT 
AACAAGACTG ATGTGAAGGA AAAACAGAAA AGGCTTGTTG AACAACTCAC TGGATTAATA 
AGTAGTTCTC CTGGCCCCCC AACAAGGAAA TTACTAGCTA AAAATCTTGC AGCTCTTTAT 
AGCATTGGAG ATACTTTCAC TGTTTTTCAA ACTCTTGACA AGTGTAATGA TATTATCAAA 
AACAAAGATG ATACTGCAGC CTATTTGCCA ACCAAACTGG CTGCAGTAGC ATGTGTTGGG 
GCATTTTATG AAAAAATGGG GAGGATGTTA GGAAGTGCAT TTCCAGAAAC AGTTAACAAT 
CTTTTGAAGT CTCTGAAAAA TGCAGAGTCT CAAGGCCGAA GTGAAATCTT AATGAGTTTA 
CAAAAAGTCC TAAATGGACT GGGTGGTGCA GCAGCTTCTT GTCATCGTGA TATTTATAAG 
AATGCAAGAT CTCTGCTAAG TGACAGATCT ATGGCTGTTA GGTGTGCAGT TGCTAAATGT 
CTGCTAGAAC TACAAAATGA AGCTGTATTT ATGTGGACAG CTGAATTAGA AAATATAGCC 
ACATTATCTT TTAAGGCTTT GGAAAGCTCA AATTATGGGG TTCGAGTGGC AGTGGCAAAA 
CTCTTGGGAA CAGTCATGGC AACTGCATTG ATGCCTAAAC AGGCAATGGT AATGCGCCAG 
AATGTGAAAA GAGCCACTTT AGATGAAGTC TTAGAGCTCA TGGCCACAGG ATTTCTGCGA 
GGAGGGTCTG GATTCTTAAA GAGTGGTGGA GAAATGCTAA AAGTTGGTGG GTCAGTTAAT 
CGTGAAGTAA GAGTTGGAGT TACACAGGCA TATGTTGTTT TTGTTACAAC ATTAGGTGGT 
CAGTGGTTAG AACGCAGCTT TGCTACATTC TTATCCCATG TACTTGATCT GGTTTCACAC 
CCTCGGGCCA CTCAAACACA TGTAGAAGCT GTGTATTCAA GAAGATGTGT CTCCTTTATC 
CTCAGAGCTA CTGTAGGTAG TTTGCTTGGG GAAAAAGCTC AGATTGCAGC TGCCAAAGAA 
ATCTGCCAAG CCATTGGAAA ACAAATGAAA GCAGTGGAAG CAGTTGTCAA TGATACAAGT 
AGTGAAAACA AATCTGGAGC TGCTGACATA GCAGCAAGTC AGCATGTGAT GGTGTGTGCT 
CTGCAGGAAC TTGGAAGCTT AGTCCAAAGC TTAAATGCCA CTGCATCTCC TCTTATTCAG 
GAAGCATCTA TAGGTCTTTT GGAAACTGTA ACATCAGTGC TTCTGCATCC TAGTATGGCT 
GCCCGACTTG CTGCAGCATG GTGTTTGCGT TGTGTAGCTG TGGCCTTGCC TTTCCAATTG 
ACCCCATTTC TAGACAGATG TGCAGAAAGG CTCAATAATT TGAAGACCTC CCCTGAAGCT 
GTTAGTGGTT ACAGTTTTGC AATGGCTGCT TTATTAGGTG GAGTACATCA GTGCCCTTTG 
GGTATCCCTC ATGCCAAAGG AAAGATGGTA GTAAGCATTG CTGAAGATCT CTTACGAACT 
GCTGCTCAGA ACAGTAGGCT ATCCTTGCAA CGTACTCAAG CTGGATGGCT TCTACTTGGA 
GCACTTATGA CTTTAGGTCC TTCTGTTGTT CGTTACCATC TTCCCAAAAT GCTGTTATTA 
TGGCGAAATG TCTTCCCTCG TTCCTTAAAG GAACTAGAAG CTGAAAAGGC ACGAGGAGAT 
TCTTTTACGT GGCAGGTAAC ATTGGAAGGC CGAGCTGGAG CTTTATGTGC CATGAGGAGT 
TTTGTTGCTC ATTGTCCTGA GCTTTTAACT GAGGATGTGA TTAGAAAATT GATGACGCCT 
ATTGAATGTG CCATGACAAT GATGTCACAC ATTCCTTCTG TAATTAAAGC CCATGGAGCT 
CATTTGAAGG CCAGTGCTGC AATGGTTCGT TTAAGACTTT ACGATATTTT GGCATTGTTA 
CCCCCCAAAA CTTATGAAGG ATCTTTTAAT GCACTTCTTA GGGAGCTGGT GGCAGAATTT 
ACTTTGACAG ATAATTCTGC CAATACAACT ACTTCCCTTC TTAGATCCCT CTGCCATTAT 
GATGACAGTG TTCTTCTTGG TTCCTGGCTT CAAGAAACTG ATCATAAATC AATTGAAGAT 
CAGCTCCAAC CAAATAGTGC ATCTGGAAGT GGTGCTTTAG AGCATGACCC ATCCTCCATT 
TATTTACGAA TACCTGCTGG TGAAGCTGTT CCTGGTCCTC TTCCGCTTGG AGTCTCAGTC 
ATTGATGCTT CTGTTGCTCT CTTTGGTGTC GTATTTCCTC ATGTTTCTTA CAAACACCGA 
TTACAAATGC TGGATCATTT TGCTGAGTGT GTCAAACAGG CTAAAGGTGT TCGTCAACAG 
GCTGTACAGC TTAATATTTT CACTGCTGTT CTTAGTGCAT TAAAGGGCTT GGCTGAAAAC 
AAAAGTACTT TAGGACCTGA GGAAGTTCGG AAATCTGCTC TGACATTGGT TATGGGAGCT 
CTCGATAATC CAAATCCCAT TTTGAGATGT GCAGCTGGAG AAGCCCTTGG AAGAATGGCT 
CAAGTGGTCG GAGAAGCAAC TTTCATTGCT AGAATGGCTC AATATAGCTT TGACAAATTA 
AAATCTGCTC GAGATGTTGT TTCCAGAACT GGCCATTCAT TGGCTCTTGG CTGTCTACAT 
CGATATGTTG GGGGGATAGG ATCAGGACAA CACCTCAAGA CCAGTGTTAG TATTCTACTG 
GCACTAGCTC AAGATGGAAC TTCACCTGAA GTCCAGACCT GGTCTCTTCA TTCACTTGCT 
TTAATAGTGG ACTCTAGTGG GCCAATGTAT CGTGGCTATG TTGAACCAAC CTTGTCCCTA 
GTCCTTACAT TATTATTGAC AGTTCCACCT TCACATACAG AGGTTCATCA ATGTTTGGGT 
CGATGTTTAG GTGCTATAAT AACCACCGTT GGACCTGAAC TGCAGGGGAA TGGAGCAACA 
ATCTCAACAA TCCGCTCTTC CTGTTTGGTT GGTTGTGCAA TTACACAAGA CCATTCAGAT 
TCTCTTGTTC AGGCAGCTGC CATATCTTGC CTTCAACAAC TACATATGTT TGCACCACGA 
CATGTCAATC TATCTAGTCT GGTTCCCAGC CTTTGTGTTC ATTTATGTAG TTCCCACTTA 
TTACTCCGAA GGGCAGCAGT TGCATGTCTT CGACAACTTG CACAGCGAGA AGCTGCTGAA 
GTATGTGAAT ATGCTATGAG CCTTGCAAAA AATGCAGGAG ACAAAGAAAG CAGTGGCATC 
AATATGAATC CTTTTGCACC AGGGGTCAGT TCTCGCCGTG ATATCCATTG TCGACACCAA 
GGTGTCAATA TAACAGAAAC TGGTTTAGAA GGGCTTCTTT TTGGAATGCT AGACCGGGAG 
ACTGATAGAA AATTATGTTC GGATATCCAT GATACTTTAG GACATATGCT TTCATCTCTG 
GCTGTAGACA AACTTTCACA TTGGCTAATG CTTTGTAAGG ATGTTCTGGC AGCATCCAGT 
GATATGAGCA CTACAACCCC TTTAAGTAGT GGAAAGGATG AAGAATCTGA GAAGAAGGAT 
GAGATGGATG ATGATACTAT GTTTACCACA TTAGGTGAAG AGGATAAATC AAAGCCATCT 
GTAGCTCCTC GCTGGGCTAC TCGAGTGTTT GCTGCTGACT GCTTGTGTCG AGTCATCATG 
TTGTGTGAGA ATGCAGATCA GGCTCATTTT GATCTTGCTA TGGCACGTTC AGCCAAATTG 
AAAAATCCTG CAAATGATCT TCTAGTGCTC CATCTCTCTG ACCTGATACG CATGGCATTC 
ATGGCTGCTA CAGATCACAG CAACCAGCTG CGGATGGCTG GACTCCAAGC ACTTGAGGAC 
ATTATCAAGA AATTTGCATC CGTGCCTGAG CCAGAATTTC CAGGTCATGT GATTTTGGAG 
CAGTACCAAG CTAATGTGGG AGCTGCTCTC AGACCAGCTT TTTCACAAGA TACTCCATCA 
GACATAATTG CAAAAGCTTG CCAGGTTTGT AGTACATGGA TTGGAAGTGG AGTTGTCAGT 
GATCTCAATG ATCTCCGTCG AGTACACAAT TTGCTTGTTT CCTCACTTGA CAAAGTACAG 
GCAGGAAAAG GATCTTCCAG CCAACTTTAT AGAGAGAGTG CAACAACCAT GGAAAAATTG 
GCAGTGCTAA AAGCTTGGGC TGAGGTTTAT GTTGTTGCCA TGAATATTAA AAAGGAAGCA 
GAAACTAAAC CAAAAAGAGC CATAAAAAAT ACTGATGATG AAGATGATGA TTATGGGACC 
ATAGATGAGT TGCCACCAGA CAGTTTAATT ACACTTGTAC AACCTGAATT GCCAACACTT 
AGCCGCCTCT GGTTGGCAGC ATTAAAAGAT TATGCACTTT TGACTTTACC AGCTGAATTT 
TCAAGTCAGC TTCCACCAGA TGGGGGAGCA TTTTATACTC CAGAGACGAT TGATACGGCT 
CGACTTCACT ATCGGAACTC TTGGGCTCCA ATTCTCCATG CAGTAGCACT TTGGTTAAAT 
AGCACAGGAT TTACTAATTC AGAAGCAACA GAAGAAACAT CAGTATCTGG TTTGCAAAAA 
CGTCCAACAC CTGTCATTTT AAACCAAATA CCTGGAGCAA CAGCTAATGC TAAATCTTTG 
CCAGAAATCA ACAAGGACAG AATGCATCTG ATTTTAGGTG TTAGTATACA GTTTCTCTGT 
TCCCCAAGAC CTGAGGAACC TATTGAACAT GTTACAGCCT GTCTGCAGGC ATTGCATACC 
TTGCTGGAAT CCCCTTGTGC CAGAATTCAT ATTGGAGAAG ATCAGCTCAT TGGTGTTGAG 
TTGCTGAATG TGCTACATCG ACTCCTTTTG ACTTGGAATC CACCCTCAGT TCAGCTGTTG 
GTTACTGGAG TGGTACAGCA GATAGTTAGA GCAGCTCAAG ATTATTTGCA AGAAAAAAGA 
AACACTCTAA ATGAAGATGA TATGGAAAAA GAGAGTTGCC CTGTTCTAGG AGAAGGAGGT 
GACAATGGTG GTCTTGTGCC TGGAAAATCT CTTGTCTTTG CAACCATGGA GCTATTGATG 
TTCATCTTAG TACGGCACAT GCCACATCTC AGTACCAAGA TGTCAGATTC ACCAAGTCAT 
ATATCCACTA AAACTCGATT ATCTGAAGAA AGTGCTCGTT TAGTGGCAGC GGCAGTTACC 
ATCCTCTCAG ACTTGCCATC TCTTTGCTCT CCTGCTGGAT GTATGACAAT ATTACCCACA 
ATTCTCTTTC TGATTGCAAG AATATTGAAA GATACTGCAA TAAAGTCTGG TGATAATCAA 
GTACCCCCTC CAGTTAATGC AGCTCTACAA GGAATTAAAA ACATTGTGAC TCTCTCAATG 
GCCAAAACTG ATGAAACGCA AAAACAGTGG ACTGATCTAA TTCGTAGCAC TCTTGCATGT 
ATCCTGGAAT ATTCTCAACC AGAAGATTCG ATGCCAATTC CCGATGAAGT GAGCATGCTT 
ACAGCCATTG CACTCTTTCT ATGGTCTGCT AGTACTGAAA TAATTGGAGT TCAGTCATTA 
CAGAATGGTT GCATGAACAG ATTTAAAAAT GCACTGAATT CTTGTGATCC TTGGGTTCAA 
GCTAAGTGTT ACCAGCTTCT TCTTTCAGTC TTCCAGCATT CCAACCGTGC CCTTTCAACT 
CCCTACATCC ATTCATTAGC TCCAATAATG GTTGAAAAAC TAAAAGCTGT TGAAAGGAGC 
CGGCCAACCA GTAGTACAGA GCTTTTAGCT GTTCAAGAAG GAATCAAAGT TCTTGAAACA 
TTGGTTGCCC TTGGTGAAGA GCAGAACAGA GTCCAGCTAC TTGCTCTTTT AGTTCCAACT 
TTGATTTCAT ATTTGTTAGA TGAAAATGCT TTTGATTCAG CACTTACGGC ATCCAAAGAT 
CTTCATGAAT TTGCACTCCA GAATTTAATG CACATTGGGC CTCTCTATCC ACATGCTTTC 
AAGACTGTTA TGGGAGCTGC TCCTGAATTG AAAACAAGAC TCGAAACTGC AGTTCGCGCA 
AGTCAAGCTA GTAAAGCAAA AGCAGCTGCC AGGCAACCAG CACCTACCAT ACATTCCGCT 
CCAACAATTA AACTCAAAAC AAGCTTTTTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5B ( XM_031951271.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031807131.1 Sarcophilus harrisii HEAT repeat containing 5B (HEATR5B), transcript variant X2, mRNA.

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CloneID	OSa224488
Clone ID Related Accession (Same CDS sequence)	XM_031951271.1
Accession Version	XM_031951271.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	HEAT repeat-containing protein 5B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCCCA TATCAGTAAG TCACACACAC CGTGATTCAA GAATTTCACC AATAATGGAG 
TTGGCCCATA GTTTATTGCT AAATGAAGAA GCTTTGGCCC AAATTACAGA AGCAAAGAGG 
CCTGTTTTTA TCTTTGAGTG GTTGCGATTT CTTGACAAAG TCCTGGTAGC TGCTAACAAG 
ACTGATGTGA AGGAAAAACA GAAAAGGCTT GTTGAACAAC TCACTGGATT AATAAGTAGT 
TCTCCTGGCC CCCCAACAAG GAAATTACTA GCTAAAAATC TTGCAGCTCT TTATAGCATT 
GGAGATACTT TCACTGTTTT TCAAACTCTT GACAAGTGTA ATGATATTAT CAAAAACAAA 
GATGATACTG CAGCCTATTT GCCAACCAAA CTGGCTGCAG TAGCATGTGT TGGGGCATTT 
TATGAAAAAA TGGGGAGGAT GTTAGGAAGT GCATTTCCAG AAACAGTTAA CAATCTTTTG 
AAGTCTCTGA AAAATGCAGA GTCTCAAGGC CGAAGTGAAA TCTTAATGAG TTTACAAAAA 
GTCCTAAATG GACTGGGTGG TGCAGCAGCT TCTTGTCATC GTGATATTTA TAAGAATGCA 
AGATCTCTGC TAAGTGACAG ATCTATGGCT GTTAGGTGTG CAGTTGCTAA ATGTCTGCTA 
GAACTACAAA ATGAAGCTGT ATTTATGTGG ACAGCTGAAT TAGAAAATAT AGCCACATTA 
TCTTTTAAGG CTTTGGAAAG CTCAAATTAT GGGGTTCGAG TGGCAGTGGC AAAACTCTTG 
GGAACAGTCA TGGCAACTGC ATTGATGCCT AAACAGGCAA TGGTAATGCG CCAGAATGTG 
AAAAGAGCCA CTTTAGATGA AGTCTTAGAG CTCATGGCCA CAGGATTTCT GCGAGGAGGG 
TCTGGATTCT TAAAGAGTGG TGGAGAAATG CTAAAAGTTG GTGGGTCAGT TAATCGTGAA 
GTAAGAGTTG GAGTTACACA GGCATATGTT GTTTTTGTTA CAACATTAGG TGGTCAGTGG 
TTAGAACGCA GCTTTGCTAC ATTCTTATCC CATGTACTTG ATCTGGTTTC ACACCCTCGG 
GCCACTCAAA CACATGTAGA AGCTGTGTAT TCAAGAAGAT GTGTCTCCTT TATCCTCAGA 
GCTACTGTAG GTAGTTTGCT TGGGGAAAAA GCTCAGATTG CAGCTGCCAA AGAAATCTGC 
CAAGCCATTG GAAAACAAAT GAAAGCAGTG GAAGCAGTTG TCAATGATAC AAGTAGTGAA 
AACAAATCTG GAGCTGCTGA CATAGCAGCA AGTCAGCATG TGATGGTGTG TGCTCTGCAG 
GAACTTGGAA GCTTAGTCCA AAGCTTAAAT GCCACTGCAT CTCCTCTTAT TCAGGAAGCA 
TCTATAGGTC TTTTGGAAAC TGTAACATCA GTGCTTCTGC ATCCTAGTAT GGCTGCCCGA 
CTTGCTGCAG CATGGTGTTT GCGTTGTGTA GCTGTGGCCT TGCCTTTCCA ATTGACCCCA 
TTTCTAGACA GATGTGCAGA AAGGCTCAAT AATTTGAAGA CCTCCCCTGA AGCTGTTAGT 
GGTTACAGTT TTGCAATGGC TGCTTTATTA GGTGGAGTAC ATCAGTGCCC TTTGGGTATC 
CCTCATGCCA AAGGAAAGAT GGTAGTAAGC ATTGCTGAAG ATCTCTTACG AACTGCTGCT 
CAGAACAGTA GGCTATCCTT GCAACGTACT CAAGCTGGAT GGCTTCTACT TGGAGCACTT 
ATGACTTTAG GTCCTTCTGT TGTTCGTTAC CATCTTCCCA AAATGCTGTT ATTATGGCGA 
AATGTCTTCC CTCGTTCCTT AAAGGAACTA GAAGCTGAAA AGGCACGAGG AGATTCTTTT 
ACGTGGCAGG TAACATTGGA AGGCCGAGCT GGAGCTTTAT GTGCCATGAG GAGTTTTGTT 
GCTCATTGTC CTGAGCTTTT AACTGAGGAT GTGATTAGAA AATTGATGAC GCCTATTGAA 
TGTGCCATGA CAATGATGTC ACACATTCCT TCTGTAATTA AAGCCCATGG AGCTCATTTG 
AAGGCCAGTG CTGCAATGGT TCGTTTAAGA CTTTACGATA TTTTGGCATT GTTACCCCCC 
AAAACTTATG AAGGATCTTT TAATGCACTT CTTAGGGAGC TGGTGGCAGA ATTTACTTTG 
ACAGATAATT CTGCCAATAC AACTACTTCC CTTCTTAGAT CCCTCTGCCA TTATGATGAC 
AGTGTTCTTC TTGGTTCCTG GCTTCAAGAA ACTGATCATA AATCAATTGA AGATCAGCTC 
CAACCAAATA GTGCATCTGG AAGTGGTGCT TTAGAGCATG ACCCATCCTC CATTTATTTA 
CGAATACCTG CTGGTGAAGC TGTTCCTGGT CCTCTTCCGC TTGGAGTCTC AGTCATTGAT 
GCTTCTGTTG CTCTCTTTGG TGTCGTATTT CCTCATGTTT CTTACAAACA CCGATTACAA 
ATGCTGGATC ATTTTGCTGA GTGTGTCAAA CAGGCTAAAG GTGTTCGTCA ACAGGCTGTA 
CAGCTTAATA TTTTCACTGC TGTTCTTAGT GCATTAAAGG GCTTGGCTGA AAACAAAAGT 
ACTTTAGGAC CTGAGGAAGT TCGGAAATCT GCTCTGACAT TGGTTATGGG AGCTCTCGAT 
AATCCAAATC CCATTTTGAG ATGTGCAGCT GGAGAAGCCC TTGGAAGAAT GGCTCAAGTG 
GTCGGAGAAG CAACTTTCAT TGCTAGAATG GCTCAATATA GCTTTGACAA ATTAAAATCT 
GCTCGAGATG TTGTTTCCAG AACTGGCCAT TCATTGGCTC TTGGCTGTCT ACATCGATAT 
GTTGGGGGGA TAGGATCAGG ACAACACCTC AAGACCAGTG TTAGTATTCT ACTGGCACTA 
GCTCAAGATG GAACTTCACC TGAAGTCCAG ACCTGGTCTC TTCATTCACT TGCTTTAATA 
GTGGACTCTA GTGGGCCAAT GTATCGTGGC TATGTTGAAC CAACCTTGTC CCTAGTCCTT 
ACATTATTAT TGACAGTTCC ACCTTCACAT ACAGAGGTTC ATCAATGTTT GGGTCGATGT 
TTAGGTGCTA TAATAACCAC CGTTGGACCT GAACTGCAGG GGAATGGAGC AACAATCTCA 
ACAATCCGCT CTTCCTGTTT GGTTGGTTGT GCAATTACAC AAGACCATTC AGATTCTCTT 
GTTCAGGCAG CTGCCATATC TTGCCTTCAA CAACTACATA TGTTTGCACC ACGACATGTC 
AATCTATCTA GTCTGGTTCC CAGCCTTTGT GTTCATTTAT GTAGTTCCCA CTTATTACTC 
CGAAGGGCAG CAGTTGCATG TCTTCGACAA CTTGCACAGC GAGAAGCTGC TGAAGTATGT 
GAATATGCTA TGAGCCTTGC AAAAAATGCA GGAGACAAAG AAAGCAGTGG CATCAATATG 
AATCCTTTTG CACCAGGGGT CAGTTCTCGC CGTGATATCC ATTGTCGACA CCAAGGTGTC 
AATATAACAG AAACTGGTTT AGAAGGGCTT CTTTTTGGAA TGCTAGACCG GGAGACTGAT 
AGAAAATTAT GTTCGGATAT CCATGATACT TTAGGACATA TGCTTTCATC TCTGGCTGTA 
GACAAACTTT CACATTGGCT AATGCTTTGT AAGGATGTTC TGGCAGCATC CAGTGATATG 
AGCACTACAA CCCCTTTAAG TAGTGGAAAG GATGAAGAAT CTGAGAAGAA GGATGAGATG 
GATGATGATA CTATGTTTAC CACATTAGGT GAAGAGGATA AATCAAAGCC ATCTGTAGCT 
CCTCGCTGGG CTACTCGAGT GTTTGCTGCT GACTGCTTGT GTCGAGTCAT CATGTTGTGT 
GAGAATGCAG ATCAGGCTCA TTTTGATCTT GCTATGGCAC GTTCAGCCAA ATTGAAAAAT 
CCTGCAAATG ATCTTCTAGT GCTCCATCTC TCTGACCTGA TACGCATGGC ATTCATGGCT 
GCTACAGATC ACAGCAACCA GCTGCGGATG GCTGGACTCC AAGCACTTGA GGACATTATC 
AAGAAATTTG CATCCGTGCC TGAGCCAGAA TTTCCAGGTC ATGTGATTTT GGAGCAGTAC 
CAAGCTAATG TGGGAGCTGC TCTCAGACCA GCTTTTTCAC AAGATACTCC ATCAGACATA 
ATTGCAAAAG CTTGCCAGGT TTGTAGTACA TGGATTGGAA GTGGAGTTGT CAGTGATCTC 
AATGATCTCC GTCGAGTACA CAATTTGCTT GTTTCCTCAC TTGACAAAGT ACAGGCAGGA 
AAAGGATCTT CCAGCCAACT TTATAGAGAG AGTGCAACAA CCATGGAAAA ATTGGCAGTG 
CTAAAAGCTT GGGCTGAGGT TTATGTTGTT GCCATGAATA TTAAAAAGGA AGCAGAAACT 
AAACCAAAAA GAGCCATAAA AAATACTGAT GATGAAGATG ATGATTATGG GACCATAGAT 
GAGTTGCCAC CAGACAGTTT AATTACACTT GTACAACCTG AATTGCCAAC ACTTAGCCGC 
CTCTGGTTGG CAGCATTAAA AGATTATGCA CTTTTGACTT TACCAGCTGA ATTTTCAAGT 
CAGCTTCCAC CAGATGGGGG AGCATTTTAT ACTCCAGAGA CGATTGATAC GGCTCGACTT 
CACTATCGGA ACTCTTGGGC TCCAATTCTC CATGCAGTAG CACTTTGGTT AAATAGCACA 
GGATTTACTA ATTCAGAAGC AACAGAAGAA ACATCAGTAT CTGGTTTGCA AAAACGTCCA 
ACACCTGTCA TTTTAAACCA AATACCTGGA GCAACAGCTA ATGCTAAATC TTTGCCAGAA 
ATCAACAAGG ACAGAATGCA TCTGATTTTA GGTGTTAGTA TACAGTTTCT CTGTTCCCCA 
AGACCTGAGG AACCTATTGA ACATGTTACA GCCTGTCTGC AGGCATTGCA TACCTTGCTG 
GAATCCCCTT GTGCCAGAAT TCATATTGGA GAAGATCAGC TCATTGGTGT TGAGTTGCTG 
AATGTGCTAC ATCGACTCCT TTTGACTTGG AATCCACCCT CAGTTCAGCT GTTGGTTACT 
GGAGTGGTAC AGCAGATAGT TAGAGCAGCT CAAGATTATT TGCAAGAAAA AAGAAACACT 
CTAAATGAAG ATGATATGGA AAAAGAGAGT TGCCCTGTTC TAGGAGAAGG AGGTGACAAT 
GGTGGTCTTG TGCCTGGAAA ATCTCTTGTC TTTGCAACCA TGGAGCTATT GATGTTCATC 
TTAGTACGGC ACATGCCACA TCTCAGTACC AAGATGTCAG ATTCACCAAG TCATATATCC 
ACTAAAACTC GATTATCTGA AGAAAGTGCT CGTTTAGTGG CAGCGGCAGT TACCATCCTC 
TCAGACTTGC CATCTCTTTG CTCTCCTGCT GGATGTATGA CAATATTACC CACAATTCTC 
TTTCTGATTG CAAGAATATT GAAAGATACT GCAATAAAGT CTGGTGATAA TCAAGTACCC 
CCTCCAGTTA ATGCAGCTCT ACAAGGAATT AAAAACATTG TGACTCTCTC AATGGCCAAA 
ACTGATGAAA CGCAAAAACA GTGGACTGAT CTAATTCGTA GCACTCTTGC ATGTATCCTG 
GAATATTCTC AACCAGAAGA TTCGATGCCA ATTCCCGATG AAGTGAGCAT GCTTACAGCC 
ATTGCACTCT TTCTATGGTC TGCTAGTACT GAAATAATTG GAGTTCAGTC ATTACAGAAT 
GGTTGCATGA ACAGATTTAA AAATGCACTG AATTCTTGTG ATCCTTGGGT TCAAGCTAAG 
TGTTACCAGC TTCTTCTTTC AGTCTTCCAG CATTCCAACC GTGCCCTTTC AACTCCCTAC 
ATCCATTCAT TAGCTCCAAT AATGGTTGAA AAACTAAAAG CTGTTGAAAG GAGCCGGCCA 
ACCAGTAGTA CAGAGCTTTT AGCTGTTCAA GAAGGAATCA AAGTTCTTGA AACATTGGTT 
GCCCTTGGTG AAGAGCAGAA CAGAGTCCAG CTACTTGCTC TTTTAGTTCC AACTTTGATT 
TCATATTTGT TAGATGAAAA TGCTTTTGAT TCAGCACTTA CGGCATCCAA AGATCTTCAT 
GAATTTGCAC TCCAGAATTT AATGCACATT GGGCCTCTCT ATCCACATGC TTTCAAGACT 
GTTATGGGAG CTGCTCCTGA ATTGAAAACA AGACTCGAAA CTGCAGTTCG CGCAAGTCAA 
GCTAGTAAAG CAAAAGCAGC TGCCAGGCAA CCAGCACCTA CCATACATTC CGCTCCAACA 
ATTAAACTCA AAACAAGCTT TTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031807131.1
CDS	289..6555
Translation	MTPISVSHTHRDSRISPIMELAHSLLLNEEALAQITEAKRPVFIFEWLRFLDKVLVAANKTDVKEKQKRLVEQLTGLISSSPGPPTRKLLAKNLAALYSIGDTFTVFQTLDKCNDIIKNKDDTAAYLPTKLAAVACVGAFYEKMGRMLGSAFPETVNNLLKSLKNAESQGRSEILMSLQKVLNGLGGAAASCHRDIYKNARSLLSDRSMAVRCAVAKCLLELQNEAVFMWTAELENIATLSFKALESSNYGVRVAVAKLLGTVMATALMPKQAMVMRQNVKRATLDEVLELMATGFLRGGSGFLKSGGEMLKVGGSVNREVRVGVTQAYVVFVTTLGGQWLERSFATFLSHVLDLVSHPRATQTHVEAVYSRRCVSFILRATVGSLLGEKAQIAAAKEICQAIGKQMKAVEAVVNDTSSENKSGAADIAASQHVMVCALQELGSLVQSLNATASPLIQEASIGLLETVTSVLLHPSMAARLAAAWCLRCVAVALPFQLTPFLDRCAERLNNLKTSPEAVSGYSFAMAALLGGVHQCPLGIPHAKGKMVVSIAEDLLRTAAQNSRLSLQRTQAGWLLLGALMTLGPSVVRYHLPKMLLLWRNVFPRSLKELEAEKARGDSFTWQVTLEGRAGALCAMRSFVAHCPELLTEDVIRKLMTPIECAMTMMSHIPSVIKAHGAHLKASAAMVRLRLYDILALLPPKTYEGSFNALLRELVAEFTLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAVPGPLPLGVSVIDASVALFGVVFPHVSYKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAENKSTLGPEEVRKSALTLVMGALDNPNPILRCAAGEALGRMAQVVGEATFIARMAQYSFDKLKSARDVVSRTGHSLALGCLHRYVGGIGSGQHLKTSVSILLALAQDGTSPEVQTWSLHSLALIVDSSGPMYRGYVEPTLSLVLTLLLTVPPSHTEVHQCLGRCLGAIITTVGPELQGNGATISTIRSSCLVGCAITQDHSDSLVQAAAISCLQQLHMFAPRHVNLSSLVPSLCVHLCSSHLLLRRAAVACLRQLAQREAAEVCEYAMSLAKNAGDKESSGINMNPFAPGVSSRRDIHCRHQGVNITETGLEGLLFGMLDRETDRKLCSDIHDTLGHMLSSLAVDKLSHWLMLCKDVLAASSDMSTTTPLSSGKDEESEKKDEMDDDTMFTTLGEEDKSKPSVAPRWATRVFAADCLCRVIMLCENADQAHFDLAMARSAKLKNPANDLLVLHLSDLIRMAFMAATDHSNQLRMAGLQALEDIIKKFASVPEPEFPGHVILEQYQANVGAALRPAFSQDTPSDIIAKACQVCSTWIGSGVVSDLNDLRRVHNLLVSSLDKVQAGKGSSSQLYRESATTMEKLAVLKAWAEVYVVAMNIKKEAETKPKRAIKNTDDEDDDYGTIDELPPDSLITLVQPELPTLSRLWLAALKDYALLTLPAEFSSQLPPDGGAFYTPETIDTARLHYRNSWAPILHAVALWLNSTGFTNSEATEETSVSGLQKRPTPVILNQIPGATANAKSLPEINKDRMHLILGVSIQFLCSPRPEEPIEHVTACLQALHTLLESPCARIHIGEDQLIGVELLNVLHRLLLTWNPPSVQLLVTGVVQQIVRAAQDYLQEKRNTLNEDDMEKESCPVLGEGGDNGGLVPGKSLVFATMELLMFILVRHMPHLSTKMSDSPSHISTKTRLSEESARLVAAAVTILSDLPSLCSPAGCMTILPTILFLIARILKDTAIKSGDNQVPPPVNAALQGIKNIVTLSMAKTDETQKQWTDLIRSTLACILEYSQPEDSMPIPDEVSMLTAIALFLWSASTEIIGVQSLQNGCMNRFKNALNSCDPWVQAKCYQLLLSVFQHSNRALSTPYIHSLAPIMVEKLKAVERSRPTSSTELLAVQEGIKVLETLVALGEEQNRVQLLALLVPTLISYLLDENAFDSALTASKDLHEFALQNLMHIGPLYPHAFKTVMGAAPELKTRLETAVRASQASKAKAAARQPAPTIHSAPTIKLKTSFF

Target ORF information:

RefSeq Version	XM_031951271.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HEAT repeat containing 5B (HEATR5B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031951271.1

ORF Insert Sequence:

ATGACTCCCA TATCAGTAAG TCACACACAC CGTGATTCAA GAATTTCACC AATAATGGAG 
TTGGCCCATA GTTTATTGCT AAATGAAGAA GCTTTGGCCC AAATTACAGA AGCAAAGAGG 
CCTGTTTTTA TCTTTGAGTG GTTGCGATTT CTTGACAAAG TCCTGGTAGC TGCTAACAAG 
ACTGATGTGA AGGAAAAACA GAAAAGGCTT GTTGAACAAC TCACTGGATT AATAAGTAGT 
TCTCCTGGCC CCCCAACAAG GAAATTACTA GCTAAAAATC TTGCAGCTCT TTATAGCATT 
GGAGATACTT TCACTGTTTT TCAAACTCTT GACAAGTGTA ATGATATTAT CAAAAACAAA 
GATGATACTG CAGCCTATTT GCCAACCAAA CTGGCTGCAG TAGCATGTGT TGGGGCATTT 
TATGAAAAAA TGGGGAGGAT GTTAGGAAGT GCATTTCCAG AAACAGTTAA CAATCTTTTG 
AAGTCTCTGA AAAATGCAGA GTCTCAAGGC CGAAGTGAAA TCTTAATGAG TTTACAAAAA 
GTCCTAAATG GACTGGGTGG TGCAGCAGCT TCTTGTCATC GTGATATTTA TAAGAATGCA 
AGATCTCTGC TAAGTGACAG ATCTATGGCT GTTAGGTGTG CAGTTGCTAA ATGTCTGCTA 
GAACTACAAA ATGAAGCTGT ATTTATGTGG ACAGCTGAAT TAGAAAATAT AGCCACATTA 
TCTTTTAAGG CTTTGGAAAG CTCAAATTAT GGGGTTCGAG TGGCAGTGGC AAAACTCTTG 
GGAACAGTCA TGGCAACTGC ATTGATGCCT AAACAGGCAA TGGTAATGCG CCAGAATGTG 
AAAAGAGCCA CTTTAGATGA AGTCTTAGAG CTCATGGCCA CAGGATTTCT GCGAGGAGGG 
TCTGGATTCT TAAAGAGTGG TGGAGAAATG CTAAAAGTTG GTGGGTCAGT TAATCGTGAA 
GTAAGAGTTG GAGTTACACA GGCATATGTT GTTTTTGTTA CAACATTAGG TGGTCAGTGG 
TTAGAACGCA GCTTTGCTAC ATTCTTATCC CATGTACTTG ATCTGGTTTC ACACCCTCGG 
GCCACTCAAA CACATGTAGA AGCTGTGTAT TCAAGAAGAT GTGTCTCCTT TATCCTCAGA 
GCTACTGTAG GTAGTTTGCT TGGGGAAAAA GCTCAGATTG CAGCTGCCAA AGAAATCTGC 
CAAGCCATTG GAAAACAAAT GAAAGCAGTG GAAGCAGTTG TCAATGATAC AAGTAGTGAA 
AACAAATCTG GAGCTGCTGA CATAGCAGCA AGTCAGCATG TGATGGTGTG TGCTCTGCAG 
GAACTTGGAA GCTTAGTCCA AAGCTTAAAT GCCACTGCAT CTCCTCTTAT TCAGGAAGCA 
TCTATAGGTC TTTTGGAAAC TGTAACATCA GTGCTTCTGC ATCCTAGTAT GGCTGCCCGA 
CTTGCTGCAG CATGGTGTTT GCGTTGTGTA GCTGTGGCCT TGCCTTTCCA ATTGACCCCA 
TTTCTAGACA GATGTGCAGA AAGGCTCAAT AATTTGAAGA CCTCCCCTGA AGCTGTTAGT 
GGTTACAGTT TTGCAATGGC TGCTTTATTA GGTGGAGTAC ATCAGTGCCC TTTGGGTATC 
CCTCATGCCA AAGGAAAGAT GGTAGTAAGC ATTGCTGAAG ATCTCTTACG AACTGCTGCT 
CAGAACAGTA GGCTATCCTT GCAACGTACT CAAGCTGGAT GGCTTCTACT TGGAGCACTT 
ATGACTTTAG GTCCTTCTGT TGTTCGTTAC CATCTTCCCA AAATGCTGTT ATTATGGCGA 
AATGTCTTCC CTCGTTCCTT AAAGGAACTA GAAGCTGAAA AGGCACGAGG AGATTCTTTT 
ACGTGGCAGG TAACATTGGA AGGCCGAGCT GGAGCTTTAT GTGCCATGAG GAGTTTTGTT 
GCTCATTGTC CTGAGCTTTT AACTGAGGAT GTGATTAGAA AATTGATGAC GCCTATTGAA 
TGTGCCATGA CAATGATGTC ACACATTCCT TCTGTAATTA AAGCCCATGG AGCTCATTTG 
AAGGCCAGTG CTGCAATGGT TCGTTTAAGA CTTTACGATA TTTTGGCATT GTTACCCCCC 
AAAACTTATG AAGGATCTTT TAATGCACTT CTTAGGGAGC TGGTGGCAGA ATTTACTTTG 
ACAGATAATT CTGCCAATAC AACTACTTCC CTTCTTAGAT CCCTCTGCCA TTATGATGAC 
AGTGTTCTTC TTGGTTCCTG GCTTCAAGAA ACTGATCATA AATCAATTGA AGATCAGCTC 
CAACCAAATA GTGCATCTGG AAGTGGTGCT TTAGAGCATG ACCCATCCTC CATTTATTTA 
CGAATACCTG CTGGTGAAGC TGTTCCTGGT CCTCTTCCGC TTGGAGTCTC AGTCATTGAT 
GCTTCTGTTG CTCTCTTTGG TGTCGTATTT CCTCATGTTT CTTACAAACA CCGATTACAA 
ATGCTGGATC ATTTTGCTGA GTGTGTCAAA CAGGCTAAAG GTGTTCGTCA ACAGGCTGTA 
CAGCTTAATA TTTTCACTGC TGTTCTTAGT GCATTAAAGG GCTTGGCTGA AAACAAAAGT 
ACTTTAGGAC CTGAGGAAGT TCGGAAATCT GCTCTGACAT TGGTTATGGG AGCTCTCGAT 
AATCCAAATC CCATTTTGAG ATGTGCAGCT GGAGAAGCCC TTGGAAGAAT GGCTCAAGTG 
GTCGGAGAAG CAACTTTCAT TGCTAGAATG GCTCAATATA GCTTTGACAA ATTAAAATCT 
GCTCGAGATG TTGTTTCCAG AACTGGCCAT TCATTGGCTC TTGGCTGTCT ACATCGATAT 
GTTGGGGGGA TAGGATCAGG ACAACACCTC AAGACCAGTG TTAGTATTCT ACTGGCACTA 
GCTCAAGATG GAACTTCACC TGAAGTCCAG ACCTGGTCTC TTCATTCACT TGCTTTAATA 
GTGGACTCTA GTGGGCCAAT GTATCGTGGC TATGTTGAAC CAACCTTGTC CCTAGTCCTT 
ACATTATTAT TGACAGTTCC ACCTTCACAT ACAGAGGTTC ATCAATGTTT GGGTCGATGT 
TTAGGTGCTA TAATAACCAC CGTTGGACCT GAACTGCAGG GGAATGGAGC AACAATCTCA 
ACAATCCGCT CTTCCTGTTT GGTTGGTTGT GCAATTACAC AAGACCATTC AGATTCTCTT 
GTTCAGGCAG CTGCCATATC TTGCCTTCAA CAACTACATA TGTTTGCACC ACGACATGTC 
AATCTATCTA GTCTGGTTCC CAGCCTTTGT GTTCATTTAT GTAGTTCCCA CTTATTACTC 
CGAAGGGCAG CAGTTGCATG TCTTCGACAA CTTGCACAGC GAGAAGCTGC TGAAGTATGT 
GAATATGCTA TGAGCCTTGC AAAAAATGCA GGAGACAAAG AAAGCAGTGG CATCAATATG 
AATCCTTTTG CACCAGGGGT CAGTTCTCGC CGTGATATCC ATTGTCGACA CCAAGGTGTC 
AATATAACAG AAACTGGTTT AGAAGGGCTT CTTTTTGGAA TGCTAGACCG GGAGACTGAT 
AGAAAATTAT GTTCGGATAT CCATGATACT TTAGGACATA TGCTTTCATC TCTGGCTGTA 
GACAAACTTT CACATTGGCT AATGCTTTGT AAGGATGTTC TGGCAGCATC CAGTGATATG 
AGCACTACAA CCCCTTTAAG TAGTGGAAAG GATGAAGAAT CTGAGAAGAA GGATGAGATG 
GATGATGATA CTATGTTTAC CACATTAGGT GAAGAGGATA AATCAAAGCC ATCTGTAGCT 
CCTCGCTGGG CTACTCGAGT GTTTGCTGCT GACTGCTTGT GTCGAGTCAT CATGTTGTGT 
GAGAATGCAG ATCAGGCTCA TTTTGATCTT GCTATGGCAC GTTCAGCCAA ATTGAAAAAT 
CCTGCAAATG ATCTTCTAGT GCTCCATCTC TCTGACCTGA TACGCATGGC ATTCATGGCT 
GCTACAGATC ACAGCAACCA GCTGCGGATG GCTGGACTCC AAGCACTTGA GGACATTATC 
AAGAAATTTG CATCCGTGCC TGAGCCAGAA TTTCCAGGTC ATGTGATTTT GGAGCAGTAC 
CAAGCTAATG TGGGAGCTGC TCTCAGACCA GCTTTTTCAC AAGATACTCC ATCAGACATA 
ATTGCAAAAG CTTGCCAGGT TTGTAGTACA TGGATTGGAA GTGGAGTTGT CAGTGATCTC 
AATGATCTCC GTCGAGTACA CAATTTGCTT GTTTCCTCAC TTGACAAAGT ACAGGCAGGA 
AAAGGATCTT CCAGCCAACT TTATAGAGAG AGTGCAACAA CCATGGAAAA ATTGGCAGTG 
CTAAAAGCTT GGGCTGAGGT TTATGTTGTT GCCATGAATA TTAAAAAGGA AGCAGAAACT 
AAACCAAAAA GAGCCATAAA AAATACTGAT GATGAAGATG ATGATTATGG GACCATAGAT 
GAGTTGCCAC CAGACAGTTT AATTACACTT GTACAACCTG AATTGCCAAC ACTTAGCCGC 
CTCTGGTTGG CAGCATTAAA AGATTATGCA CTTTTGACTT TACCAGCTGA ATTTTCAAGT 
CAGCTTCCAC CAGATGGGGG AGCATTTTAT ACTCCAGAGA CGATTGATAC GGCTCGACTT 
CACTATCGGA ACTCTTGGGC TCCAATTCTC CATGCAGTAG CACTTTGGTT AAATAGCACA 
GGATTTACTA ATTCAGAAGC AACAGAAGAA ACATCAGTAT CTGGTTTGCA AAAACGTCCA 
ACACCTGTCA TTTTAAACCA AATACCTGGA GCAACAGCTA ATGCTAAATC TTTGCCAGAA 
ATCAACAAGG ACAGAATGCA TCTGATTTTA GGTGTTAGTA TACAGTTTCT CTGTTCCCCA 
AGACCTGAGG AACCTATTGA ACATGTTACA GCCTGTCTGC AGGCATTGCA TACCTTGCTG 
GAATCCCCTT GTGCCAGAAT TCATATTGGA GAAGATCAGC TCATTGGTGT TGAGTTGCTG 
AATGTGCTAC ATCGACTCCT TTTGACTTGG AATCCACCCT CAGTTCAGCT GTTGGTTACT 
GGAGTGGTAC AGCAGATAGT TAGAGCAGCT CAAGATTATT TGCAAGAAAA AAGAAACACT 
CTAAATGAAG ATGATATGGA AAAAGAGAGT TGCCCTGTTC TAGGAGAAGG AGGTGACAAT 
GGTGGTCTTG TGCCTGGAAA ATCTCTTGTC TTTGCAACCA TGGAGCTATT GATGTTCATC 
TTAGTACGGC ACATGCCACA TCTCAGTACC AAGATGTCAG ATTCACCAAG TCATATATCC 
ACTAAAACTC GATTATCTGA AGAAAGTGCT CGTTTAGTGG CAGCGGCAGT TACCATCCTC 
TCAGACTTGC CATCTCTTTG CTCTCCTGCT GGATGTATGA CAATATTACC CACAATTCTC 
TTTCTGATTG CAAGAATATT GAAAGATACT GCAATAAAGT CTGGTGATAA TCAAGTACCC 
CCTCCAGTTA ATGCAGCTCT ACAAGGAATT AAAAACATTG TGACTCTCTC AATGGCCAAA 
ACTGATGAAA CGCAAAAACA GTGGACTGAT CTAATTCGTA GCACTCTTGC ATGTATCCTG 
GAATATTCTC AACCAGAAGA TTCGATGCCA ATTCCCGATG AAGTGAGCAT GCTTACAGCC 
ATTGCACTCT TTCTATGGTC TGCTAGTACT GAAATAATTG GAGTTCAGTC ATTACAGAAT 
GGTTGCATGA ACAGATTTAA AAATGCACTG AATTCTTGTG ATCCTTGGGT TCAAGCTAAG 
TGTTACCAGC TTCTTCTTTC AGTCTTCCAG CATTCCAACC GTGCCCTTTC AACTCCCTAC 
ATCCATTCAT TAGCTCCAAT AATGGTTGAA AAACTAAAAG CTGTTGAAAG GAGCCGGCCA 
ACCAGTAGTA CAGAGCTTTT AGCTGTTCAA GAAGGAATCA AAGTTCTTGA AACATTGGTT 
GCCCTTGGTG AAGAGCAGAA CAGAGTCCAG CTACTTGCTC TTTTAGTTCC AACTTTGATT 
TCATATTTGT TAGATGAAAA TGCTTTTGAT TCAGCACTTA CGGCATCCAA AGATCTTCAT 
GAATTTGCAC TCCAGAATTT AATGCACATT GGGCCTCTCT ATCCACATGC TTTCAAGACT 
GTTATGGGAG CTGCTCCTGA ATTGAAAACA AGACTCGAAA CTGCAGTTCG CGCAAGTCAA 
GCTAGTAAAG CAAAAGCAGC TGCCAGGCAA CCAGCACCTA CCATACATTC CGCTCCAACA 
ATTAAACTCA AAACAAGCTT TTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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HEATR5B cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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