LOC100917022 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100917022
Entrez Gene ID	100917022
Full Name	phospholipase C epsilon 1
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023495065.1	XP_023350833.1	LOW QUALITY PROTEIN: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
XM_012540289.1	XP_012395743.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
XM_023495065.2	XP_023350833.1	LOW QUALITY PROTEIN: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1

Pathways from BioSystems
Gene Ontology

The following LOC100917022 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100917022 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa116635	XM_012540289.1 Latest version!	Sarcophilus harrisii phospholipase C, epsilon 1 (PLCE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa149145	XM_023495065.2 Latest version!	Sarcophilus harrisii 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (LOC100917022), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OSa149145	XM_023495065.1	Sarcophilus harrisii phospholipase C epsilon 1 (PLCE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100917022 ( XM_012540289.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012395743.1 Sarcophilus harrisii phospholipase C, epsilon 1 (PLCE1), mRNA.

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CloneID	OSa116635
Clone ID Related Accession (Same CDS sequence)	XM_012540289.1
Accession Version	XM_012540289.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816463.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGAATGATG AAATGAACAT TGAGAAGTTA ATGATGACAA TCAACAACAC TGTGAGAAAA 
GGAGAAGAAA CAGAACGCAC TTTTGGATCA ATTTTCCATT TCTTTGGGAA GACTCTAGCC 
TTAGATATAA TCCATGGACA TATTAATATT GGCCCATGCT TGAAGCAATG TGTTCGAGAT 
ACTTCATGTG AATACCGAGC TACCTTTCAA AGGACTTCAA TTTCTCAGTA TATCACTGGT 
TCTCTATTGG AAGCAACTGC GTCTCTGGGT GCAAGAAGTG GCCTTTTAAA TACTTTTGGA 
GGATCAGTAG GAAGAACAAT GTTGAAAGAA CGTCAACCAT GTACCTCCAC AGCCAGTTCA 
ATCAACTTAC CTTCAAATGT ATCTGGTTTA AGTAACGAGC TCATAGAACT TCAACACCTC 
ATTCAGTTCC CTGAAGAAGT GGCTTGTATT CTGACTGAAC AGGAGCAGAA TATCTACCGA 
CAAGTGCTAC CTGTCGACTA CCTTTGCTTC TTAACCAGGG ACTTGGGAAA TGCTGAATGC 
CAGAGTAAAC TTCCATGCCT CAGAGCTACA GTATCTGCAT CTATTCTTAC CCCCCAGAAC 
AATGAGCACA GTGCTCTGGA AAACCTTGTA ACAAGATTTA ATGAGGTGAG TTCTTGGGTG 
ACGTGGCTGA TACTCACAGC AGGTACCATG GAAGAAAAAC GGGAAGTCTT CTCATATTTA 
GTACACATTG CAAAATGCTG CTGGAATATG GGCAACTACA ATGCTGTTAT GGAATTCTTG 
GCTGGCCTCA GGTCAAGGAA AGTTTTAAAG ATGTGGCAGT TTATGGACCA GTCTGATATT 
GAAACCATGA GAAGCCTGAA GGATGCAATG GCCCAACATG AATCTTCCTC TGAATACAAG 
AAAGTGGTAA ACCGGGCTCT GAATATCCCT GGCTGTAAAG TGGTTCCTTT CTGTGGTGTC 
TTTTTAAAGG AGCTCTGTGA AATTCTAGAT GGAACATCTG GATTTATGAA ACTTTGCCCA 
CGATATAATT CACAAGATGA AACTTTAGAG TTTGTAGCTG ATTACAATGG ACAAGATAAT 
TTTTTACAAC GAGTAGGACA AGATGGCTTA AACAACCCAG AGAAAGAATC CACTGTCAAC 
AGTATTTTTC AAATTATCCG AAGCTGCAAT CGTAGTCTGG AACCTGAAGA AGAAGACAGC 
TTAAGTGAAG GCGATGATTC AAGGAAAAAT TCCTTGAAAG ATAAAACCCG ACTACAGTTT 
GTAATTGGAG AACTGTCTGA TTCAGAAAGT GATATTTTTG AGCAGGCCAA AGAATGGGAT 
TTCCATGGAA CGGAAGAAGA GCAGAAAGCA TTCAAGCATG GCACAGAACT CATTCCCTGG 
TATGTCTTGT CAATTAGAGC TGATGTCCAT CAGTTCATAC TCCAAGGAGC CACTGTTATT 
CACTATGACC AGGATGCACA TCTGTCAGCA CGATGCTTTC TTCGACTTCA ACCTGATAAT 
AGTACTTTGA CCTGGATCAA ACCTACAACT GCCTCACTCG CCAATACTAA AGCCAAATTA 
GGCATCACAG GAAATTTAGC TGACCCTGGC AAACTTCAAT CACTTGGCAA TACTGGATTA 
AATGGACTGA TGGAAGGTTT TCTGGATTTG TTTTCAGTCA AGGCTGTGTA CATGGGACAT 
CCTGGCATTG ATATGCACAC TGTGTGTGTT CAGAACAAAC TCAGTAACAT GTCCCTCTTA 
GAGAATAGTG TGACACTGCT TTATGGCCTT CAAACTACTG AGAATAGATT ATTACACTTT 
GTGGCACCAA AACATACTGC TAAAATGTTG TATAATGGAT TATTAGAATT AACAAAAGCT 
GTAAGAAAAA TGAAGAAATT TCCTGACCAG CGACAGCAGT GGCTAAGGAA GCAATATGTC 
AGCCTCTATC AGGAGGATGG AAGATTTGAA GGTCCAACTT TGGCTCATGC TATCGAACTG 
TTTGGTGGCA GACGTTGGAC TACACGAAAT ACAAGTCCAG CAACACTTTC TAAAAGTGTG 
GAGAAACCCA TTGTGCAAAG GAACAACACC CTTGGGATAA GTGCAAATAA GAAAAAGAAA 
AAAATCCTTA TGAGGGGTGA AAGTGGAGAG GGTACTGATG ATGAGATGGC CACACGAAAG 
GCAAAAATGC ATAAGGAATG CCGTGGCCGC AGTGGCTCAG ATCCTCAAGA CCTTAATGAG 
CAAGAAGAAT CAGATCAGAA TGTTATTATT AGTCCCTGTA ATAATCTGCC ATCCAGGAGA 
GCCAATTCTT TGACTACAGC TGGATCTCCT AACTTGGCTG CTGGAGCAAC TTCACCAGTC 
AGACCACTTT CCTCTCCTGT ATTGTCACCT TCAAATAAGA ATCAGCCTAA TACATGGAGC 
AGCAGTAGTT GGCATGGCCG AGTTAAAGGT GGACTGAAGG GATTTCAAAA TTTCATGGTT 
TCAGATAGTA ACATGAGTTT TGTTGAATTT GTAGAGCTGT TCAAGTCATT CAGTGTACGA 
AGTCGAAAGG ACCTCAAGGA TCTTTTTGAC ATCTATGCTG TACCCTGCAG TCGATCAGGA 
TCTGAGTCTG TCCCACTCTA TACAAACCTG AGAATTGATG AAAATACCAG TGGCCTTCAG 
CCTGATTTAG ATTTGTTAAC CAGGAACGTG TCTGACTTGG GCTTGTTCAT TAAGAATAGG 
CAACAGTTAT CAGACAACCA GAGGCAGATA TCTGATGCCA TCGCAGCTGC TAGTATTGTG 
ACTAATGGTT CTGGAATAGA AAGCACCACG CTTGGTGTCT GTGGGGTTGG CATATTACAG 
CTGAATGACT TTTTAGTGAA TTGCCAAGGG GAACACTACA CTTATGATGA GATCCTCAGC 
ATTATCCAGA AATTCGAGCC TAGTGTGAGT ATGTATCAGC AAGGTCTAAT GTCATTTGAA 
GGATTTTCCA GGTTTCTGAT GGATAAAGAT AATTTTGCTT CCCAAAATGA TGAATCACAG 
GAGAATGTTA AAGAATTGCA GTTACCACTC TCCTATTATT ACATTGAATC TTCACACAAC 
ACATACCTCA CAGGACATCA GCTTAAAGGA GAGTCCTCAG TGGAGCTATA TAGCCAGGTC 
CTCTTGCAAG GCTGTCGGAG TGTGGAATTA GACTGTTGGG ATGGTGATGA TGGAATGCCA 
ATTATCTATC ATGGACATAC ACTTACTACC AAGATTCCCT TCAAGGAAGT AGTTGAAGCC 
ATTGATCGCA GTGCCTTCAT CAACTCTGAC TTGCCTATTA TAATATCTAT AGAAAATCAC 
TGTTCATTGC CTCAGCAACG AAAAATGGCA GAAATTTTCA AGAATGTATT TGGAGAAAAA 
CTAGTTGCCA AATTTTTGTT TGAGAGTGAT TTCTCTGATG ATCCAATGCT TCCTTCACCT 
GACCAACTTA GGAGAAAAGT ACTTCTTAAA AACAAAAAAC TGAAGGCACA TCAGACTCCA 
GTGGACATAT TAAAGCAAAA GGCTCATCAA CTGGCATCCA TGCAAACACA AAGCAATAAT 
GGTGGTAATG CCAATCCTAT GTCTGCCAAC AATGAAGAAG AAGAGGAGGA GGAGGATGAA 
TATGATTATG ACTATGAATC TCTTTCTGAT GACAACATCC TGGAAGACCG GCCTGAAAGT 
AAGTCAGCAA GTGACAAACT ACAGTTTGAG TACAATGAAG AAATTCCCAA GAGAATCAAG 
AAGGCTGACA GCTCTGTTTG CAACAAGGGA AAGGTTTATG ACATGGAACT GGGTGAAGAA 
TTTTATCTTC CACAGAATAA AAAAGAGAGT AGACAGATAG CTCCAGAGCT TTCTGACCTT 
GTCATCTACT GCCAAGCTGT AAAATTTCCA GGATTGTCAA CTTTAAACCC ATCTGGCTCT 
AACAGAGGAA AAGAAAGGAA AAGCAGGAAG TCCATTTTTG GCAACAATCC GGGCAGATTA 
AGCCCTGGGG AGACAGCAGC ATATACCAAA ACATCTGGAA AAAGTCCCTT GGACGGCGTG 
CGGCAGACCT GGGAAGAAGT CTGTTCTCTC AGCACAGCTC CGTCCCTCAG TGCCATCATC 
AGGACCCCCA GGTGCTACCA CATCTCCTCC CTGAACGAGA ATGCGGCCAA GCGGCTGTGC 
CGCCGGTACT CTCGGAAGCT GATTCAGCAC ACCGCCTGCC AGCTGCTGAG AACCTACCCT 
GCCGCCACTC GCATTGACTC CTCCAACCCC AATCCCCTCC TGTTCTGGCT GCACGGGATA 
CAGCTGGTGG CACTCAACTA CCAAACAGAC GATCTCCCCT TACACCTAAA CACAGCAATG 
TTTGAAGCTA ATGGTGGCTG TGGCTATGTT TTGAAACCCC CTGTTTTATG GGACAAGAAT 
TGTGCTATGT ACCAGCAGTT TTCTCCACTG GAAAGAGATA TGGAAAACAT GGAACCTGCT 
CTCTATTCTT TAACTATTGT CTCTGGTCAG AATGTGTGTC CTAGTAACAG TACAGGAAGT 
CCATGTATTG AAATCGATGT CCTGGGAATG CCTCTGGATA GCTGCCATTT CCGCACTAAA 
CCCATTCATC GAAATACTCT TAACCCCATG TGGAACGAAC AGTTTCTCTT CCGAGTTCAT 
TTTGAAGATC TTGTATTTCT TCGTTTTGCG GTTGTTGAAA ACAACAGTTC AGCTGTAACT 
GCCCAAAGAA TCATTCCACT GAAGGCTTTA AAAAGAGGAT ATCGGCATCT TCAGCTGAGA 
AACCTCCATA ATGAAGTCTT AGAAATCTCT AGCTTGTTCA TAAACAGCAG GAGGATGGAA 
GAGCATTCCA GTGGCAGTGC TGTGGCAGCA TCTTTGATGT TCAGCACAGA AGAAAGAAAA 
GGTCTGATGA CATACAAGGT CACAGTACAT GGGATTCCAG GTCCTGAGCC TTTTGCCATT 
TTCATTATAA ATAAAGAAAT TAAAGCAAAG CAGCTTCTTC ACCAAATTTT GACTGCTTCA 
CAAAACTCCA TACCAGTCAC TCCGGACTGT TTTTTAATGG AAGAAAAATA TTTTATATCT 
AAAGAAAAAA ATGACTTCAG GAAACAACCA TTCCAGAGAA TAATCGGTCC AGAAGAAGAG 
ATTGTGCAGA TTTTGAACAG CTGGTTCCCA GAAGAAGGTT ATGTTGGAAG GATTATTTTT 
AAAATCCAGG AGGAACGCCC AGAAGAGAAG AACATCTTCC AAGAAGAGAA GGAGGTGATG 
CTGAGCTCAG AAGATGAAAG CTTCTTTGTA CAAGTCCATG ATGTTTCTCC CGAGCAGCCC 
CGAACAGTCA TCAAAGCTCC CCGAGTCAGC ACTGCCCAGG AGGTCATCCA GCAGACTCTA 
TGCAAAGCAA AATATTCTTA CAGCATTTTG AGCAATCCCA ACCCGAGTGA CTACGTGCTC 
CTAGAGGAGG TACTAAAGGA ACCTGCTAGC AAGAAGTCTT CAACACCCAA GTCTTCTCAG 
AGAGTTCTCG GGGACCAGGA GTGTGTGTTC CAGGCTCAAA GCAGGTGGAA GGGAGCCGGG 
AAATTTATCC TTAAGCTAAA AGAGCAGGTT CAGGCAGCTC GAGATGATAA AAAGAAAGGC 
ATTTCCTTTG CTAATGAACT TAAGAAATTT GCCAAGTCTA GCAAGCAGTA TCGAGGATTC 
ACCTCACCTT CTCAGCTTTC AATAGCAGAA AGTGTCCAGA TGAAGGAAGA AAAATCTGTG 
GGCAGCTTCT CTTCTGAGAC GACAGACCAT CACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012395743.1
CDS	1..5739
Translation	MNDEMNIEKLMMTINNTVRKGEETERTFGSIFHFFGKTLALDIIHGHINIGPCLKQCVRDTSCEYRATFQRTSISQYITGSLLEATASLGARSGLLNTFGGSVGRTMLKERQPCTSTASSINLPSNVSGLSNELIELQHLIQFPEEVACILTEQEQNIYRQVLPVDYLCFLTRDLGNAECQSKLPCLRATVSASILTPQNNEHSALENLVTRFNEVSSWVTWLILTAGTMEEKREVFSYLVHIAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSSEYKKVVNRALNIPGCKVVPFCGVFLKELCEILDGTSGFMKLCPRYNSQDETLEFVADYNGQDNFLQRVGQDGLNNPEKESTVNSIFQIIRSCNRSLEPEEEDSLSEGDDSRKNSLKDKTRLQFVIGELSDSESDIFEQAKEWDFHGTEEEQKAFKHGTELIPWYVLSIRADVHQFILQGATVIHYDQDAHLSARCFLRLQPDNSTLTWIKPTTASLANTKAKLGITGNLADPGKLQSLGNTGLNGLMEGFLDLFSVKAVYMGHPGIDMHTVCVQNKLSNMSLLENSVTLLYGLQTTENRLLHFVAPKHTAKMLYNGLLELTKAVRKMKKFPDQRQQWLRKQYVSLYQEDGRFEGPTLAHAIELFGGRRWTTRNTSPATLSKSVEKPIVQRNNTLGISANKKKKKILMRGESGEGTDDEMATRKAKMHKECRGRSGSDPQDLNEQEESDQNVIISPCNNLPSRRANSLTTAGSPNLAAGATSPVRPLSSPVLSPSNKNQPNTWSSSSWHGRVKGGLKGFQNFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDIYAVPCSRSGSESVPLYTNLRIDENTSGLQPDLDLLTRNVSDLGLFIKNRQQLSDNQRQISDAIAAASIVTNGSGIESTTLGVCGVGILQLNDFLVNCQGEHYTYDEILSIIQKFEPSVSMYQQGLMSFEGFSRFLMDKDNFASQNDESQENVKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKNVFGEKLVAKFLFESDFSDDPMLPSPDQLRRKVLLKNKKLKAHQTPVDILKQKAHQLASMQTQSNNGGNANPMSANNEEEEEEEDEYDYDYESLSDDNILEDRPESKSASDKLQFEYNEEIPKRIKKADSSVCNKGKVYDMELGEEFYLPQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNPSGSNRGKERKSRKSIFGNNPGRLSPGETAAYTKTSGKSPLDGVRQTWEEVCSLSTAPSLSAIIRTPRCYHISSLNENAAKRLCRRYSRKLIQHTACQLLRTYPAATRIDSSNPNPLLFWLHGIQLVALNYQTDDLPLHLNTAMFEANGGCGYVLKPPVLWDKNCAMYQQFSPLERDMENMEPALYSLTIVSGQNVCPSNSTGSPCIEIDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFRVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEEHSSGSAVAASLMFSTEERKGLMTYKVTVHGIPGPEPFAIFIINKEIKAKQLLHQILTASQNSIPVTPDCFLMEEKYFISKEKNDFRKQPFQRIIGPEEEIVQILNSWFPEEGYVGRIIFKIQEERPEEKNIFQEEKEVMLSSEDESFFVQVHDVSPEQPRTVIKAPRVSTAQEVIQQTLCKAKYSYSILSNPNPSDYVLLEEVLKEPASKKSSTPKSSQRVLGDQECVFQAQSRWKGAGKFILKLKEQVQAARDDKKKGISFANELKKFAKSSKQYRGFTSPSQLSIAESVQMKEEKSVGSFSSETTDHHP

Target ORF information:

RefSeq Version	XM_012540289.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii phospholipase C, epsilon 1 (PLCE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012540289.1

ORF Insert Sequence:

ATGAATGATG AAATGAACAT TGAGAAGTTA ATGATGACAA TCAACAACAC TGTGAGAAAA 
GGAGAAGAAA CAGAACGCAC TTTTGGATCA ATTTTCCATT TCTTTGGGAA GACTCTAGCC 
TTAGATATAA TCCATGGACA TATTAATATT GGCCCATGCT TGAAGCAATG TGTTCGAGAT 
ACTTCATGTG AATACCGAGC TACCTTTCAA AGGACTTCAA TTTCTCAGTA TATCACTGGT 
TCTCTATTGG AAGCAACTGC GTCTCTGGGT GCAAGAAGTG GCCTTTTAAA TACTTTTGGA 
GGATCAGTAG GAAGAACAAT GTTGAAAGAA CGTCAACCAT GTACCTCCAC AGCCAGTTCA 
ATCAACTTAC CTTCAAATGT ATCTGGTTTA AGTAACGAGC TCATAGAACT TCAACACCTC 
ATTCAGTTCC CTGAAGAAGT GGCTTGTATT CTGACTGAAC AGGAGCAGAA TATCTACCGA 
CAAGTGCTAC CTGTCGACTA CCTTTGCTTC TTAACCAGGG ACTTGGGAAA TGCTGAATGC 
CAGAGTAAAC TTCCATGCCT CAGAGCTACA GTATCTGCAT CTATTCTTAC CCCCCAGAAC 
AATGAGCACA GTGCTCTGGA AAACCTTGTA ACAAGATTTA ATGAGGTGAG TTCTTGGGTG 
ACGTGGCTGA TACTCACAGC AGGTACCATG GAAGAAAAAC GGGAAGTCTT CTCATATTTA 
GTACACATTG CAAAATGCTG CTGGAATATG GGCAACTACA ATGCTGTTAT GGAATTCTTG 
GCTGGCCTCA GGTCAAGGAA AGTTTTAAAG ATGTGGCAGT TTATGGACCA GTCTGATATT 
GAAACCATGA GAAGCCTGAA GGATGCAATG GCCCAACATG AATCTTCCTC TGAATACAAG 
AAAGTGGTAA ACCGGGCTCT GAATATCCCT GGCTGTAAAG TGGTTCCTTT CTGTGGTGTC 
TTTTTAAAGG AGCTCTGTGA AATTCTAGAT GGAACATCTG GATTTATGAA ACTTTGCCCA 
CGATATAATT CACAAGATGA AACTTTAGAG TTTGTAGCTG ATTACAATGG ACAAGATAAT 
TTTTTACAAC GAGTAGGACA AGATGGCTTA AACAACCCAG AGAAAGAATC CACTGTCAAC 
AGTATTTTTC AAATTATCCG AAGCTGCAAT CGTAGTCTGG AACCTGAAGA AGAAGACAGC 
TTAAGTGAAG GCGATGATTC AAGGAAAAAT TCCTTGAAAG ATAAAACCCG ACTACAGTTT 
GTAATTGGAG AACTGTCTGA TTCAGAAAGT GATATTTTTG AGCAGGCCAA AGAATGGGAT 
TTCCATGGAA CGGAAGAAGA GCAGAAAGCA TTCAAGCATG GCACAGAACT CATTCCCTGG 
TATGTCTTGT CAATTAGAGC TGATGTCCAT CAGTTCATAC TCCAAGGAGC CACTGTTATT 
CACTATGACC AGGATGCACA TCTGTCAGCA CGATGCTTTC TTCGACTTCA ACCTGATAAT 
AGTACTTTGA CCTGGATCAA ACCTACAACT GCCTCACTCG CCAATACTAA AGCCAAATTA 
GGCATCACAG GAAATTTAGC TGACCCTGGC AAACTTCAAT CACTTGGCAA TACTGGATTA 
AATGGACTGA TGGAAGGTTT TCTGGATTTG TTTTCAGTCA AGGCTGTGTA CATGGGACAT 
CCTGGCATTG ATATGCACAC TGTGTGTGTT CAGAACAAAC TCAGTAACAT GTCCCTCTTA 
GAGAATAGTG TGACACTGCT TTATGGCCTT CAAACTACTG AGAATAGATT ATTACACTTT 
GTGGCACCAA AACATACTGC TAAAATGTTG TATAATGGAT TATTAGAATT AACAAAAGCT 
GTAAGAAAAA TGAAGAAATT TCCTGACCAG CGACAGCAGT GGCTAAGGAA GCAATATGTC 
AGCCTCTATC AGGAGGATGG AAGATTTGAA GGTCCAACTT TGGCTCATGC TATCGAACTG 
TTTGGTGGCA GACGTTGGAC TACACGAAAT ACAAGTCCAG CAACACTTTC TAAAAGTGTG 
GAGAAACCCA TTGTGCAAAG GAACAACACC CTTGGGATAA GTGCAAATAA GAAAAAGAAA 
AAAATCCTTA TGAGGGGTGA AAGTGGAGAG GGTACTGATG ATGAGATGGC CACACGAAAG 
GCAAAAATGC ATAAGGAATG CCGTGGCCGC AGTGGCTCAG ATCCTCAAGA CCTTAATGAG 
CAAGAAGAAT CAGATCAGAA TGTTATTATT AGTCCCTGTA ATAATCTGCC ATCCAGGAGA 
GCCAATTCTT TGACTACAGC TGGATCTCCT AACTTGGCTG CTGGAGCAAC TTCACCAGTC 
AGACCACTTT CCTCTCCTGT ATTGTCACCT TCAAATAAGA ATCAGCCTAA TACATGGAGC 
AGCAGTAGTT GGCATGGCCG AGTTAAAGGT GGACTGAAGG GATTTCAAAA TTTCATGGTT 
TCAGATAGTA ACATGAGTTT TGTTGAATTT GTAGAGCTGT TCAAGTCATT CAGTGTACGA 
AGTCGAAAGG ACCTCAAGGA TCTTTTTGAC ATCTATGCTG TACCCTGCAG TCGATCAGGA 
TCTGAGTCTG TCCCACTCTA TACAAACCTG AGAATTGATG AAAATACCAG TGGCCTTCAG 
CCTGATTTAG ATTTGTTAAC CAGGAACGTG TCTGACTTGG GCTTGTTCAT TAAGAATAGG 
CAACAGTTAT CAGACAACCA GAGGCAGATA TCTGATGCCA TCGCAGCTGC TAGTATTGTG 
ACTAATGGTT CTGGAATAGA AAGCACCACG CTTGGTGTCT GTGGGGTTGG CATATTACAG 
CTGAATGACT TTTTAGTGAA TTGCCAAGGG GAACACTACA CTTATGATGA GATCCTCAGC 
ATTATCCAGA AATTCGAGCC TAGTGTGAGT ATGTATCAGC AAGGTCTAAT GTCATTTGAA 
GGATTTTCCA GGTTTCTGAT GGATAAAGAT AATTTTGCTT CCCAAAATGA TGAATCACAG 
GAGAATGTTA AAGAATTGCA GTTACCACTC TCCTATTATT ACATTGAATC TTCACACAAC 
ACATACCTCA CAGGACATCA GCTTAAAGGA GAGTCCTCAG TGGAGCTATA TAGCCAGGTC 
CTCTTGCAAG GCTGTCGGAG TGTGGAATTA GACTGTTGGG ATGGTGATGA TGGAATGCCA 
ATTATCTATC ATGGACATAC ACTTACTACC AAGATTCCCT TCAAGGAAGT AGTTGAAGCC 
ATTGATCGCA GTGCCTTCAT CAACTCTGAC TTGCCTATTA TAATATCTAT AGAAAATCAC 
TGTTCATTGC CTCAGCAACG AAAAATGGCA GAAATTTTCA AGAATGTATT TGGAGAAAAA 
CTAGTTGCCA AATTTTTGTT TGAGAGTGAT TTCTCTGATG ATCCAATGCT TCCTTCACCT 
GACCAACTTA GGAGAAAAGT ACTTCTTAAA AACAAAAAAC TGAAGGCACA TCAGACTCCA 
GTGGACATAT TAAAGCAAAA GGCTCATCAA CTGGCATCCA TGCAAACACA AAGCAATAAT 
GGTGGTAATG CCAATCCTAT GTCTGCCAAC AATGAAGAAG AAGAGGAGGA GGAGGATGAA 
TATGATTATG ACTATGAATC TCTTTCTGAT GACAACATCC TGGAAGACCG GCCTGAAAGT 
AAGTCAGCAA GTGACAAACT ACAGTTTGAG TACAATGAAG AAATTCCCAA GAGAATCAAG 
AAGGCTGACA GCTCTGTTTG CAACAAGGGA AAGGTTTATG ACATGGAACT GGGTGAAGAA 
TTTTATCTTC CACAGAATAA AAAAGAGAGT AGACAGATAG CTCCAGAGCT TTCTGACCTT 
GTCATCTACT GCCAAGCTGT AAAATTTCCA GGATTGTCAA CTTTAAACCC ATCTGGCTCT 
AACAGAGGAA AAGAAAGGAA AAGCAGGAAG TCCATTTTTG GCAACAATCC GGGCAGATTA 
AGCCCTGGGG AGACAGCAGC ATATACCAAA ACATCTGGAA AAAGTCCCTT GGACGGCGTG 
CGGCAGACCT GGGAAGAAGT CTGTTCTCTC AGCACAGCTC CGTCCCTCAG TGCCATCATC 
AGGACCCCCA GGTGCTACCA CATCTCCTCC CTGAACGAGA ATGCGGCCAA GCGGCTGTGC 
CGCCGGTACT CTCGGAAGCT GATTCAGCAC ACCGCCTGCC AGCTGCTGAG AACCTACCCT 
GCCGCCACTC GCATTGACTC CTCCAACCCC AATCCCCTCC TGTTCTGGCT GCACGGGATA 
CAGCTGGTGG CACTCAACTA CCAAACAGAC GATCTCCCCT TACACCTAAA CACAGCAATG 
TTTGAAGCTA ATGGTGGCTG TGGCTATGTT TTGAAACCCC CTGTTTTATG GGACAAGAAT 
TGTGCTATGT ACCAGCAGTT TTCTCCACTG GAAAGAGATA TGGAAAACAT GGAACCTGCT 
CTCTATTCTT TAACTATTGT CTCTGGTCAG AATGTGTGTC CTAGTAACAG TACAGGAAGT 
CCATGTATTG AAATCGATGT CCTGGGAATG CCTCTGGATA GCTGCCATTT CCGCACTAAA 
CCCATTCATC GAAATACTCT TAACCCCATG TGGAACGAAC AGTTTCTCTT CCGAGTTCAT 
TTTGAAGATC TTGTATTTCT TCGTTTTGCG GTTGTTGAAA ACAACAGTTC AGCTGTAACT 
GCCCAAAGAA TCATTCCACT GAAGGCTTTA AAAAGAGGAT ATCGGCATCT TCAGCTGAGA 
AACCTCCATA ATGAAGTCTT AGAAATCTCT AGCTTGTTCA TAAACAGCAG GAGGATGGAA 
GAGCATTCCA GTGGCAGTGC TGTGGCAGCA TCTTTGATGT TCAGCACAGA AGAAAGAAAA 
GGTCTGATGA CATACAAGGT CACAGTACAT GGGATTCCAG GTCCTGAGCC TTTTGCCATT 
TTCATTATAA ATAAAGAAAT TAAAGCAAAG CAGCTTCTTC ACCAAATTTT GACTGCTTCA 
CAAAACTCCA TACCAGTCAC TCCGGACTGT TTTTTAATGG AAGAAAAATA TTTTATATCT 
AAAGAAAAAA ATGACTTCAG GAAACAACCA TTCCAGAGAA TAATCGGTCC AGAAGAAGAG 
ATTGTGCAGA TTTTGAACAG CTGGTTCCCA GAAGAAGGTT ATGTTGGAAG GATTATTTTT 
AAAATCCAGG AGGAACGCCC AGAAGAGAAG AACATCTTCC AAGAAGAGAA GGAGGTGATG 
CTGAGCTCAG AAGATGAAAG CTTCTTTGTA CAAGTCCATG ATGTTTCTCC CGAGCAGCCC 
CGAACAGTCA TCAAAGCTCC CCGAGTCAGC ACTGCCCAGG AGGTCATCCA GCAGACTCTA 
TGCAAAGCAA AATATTCTTA CAGCATTTTG AGCAATCCCA ACCCGAGTGA CTACGTGCTC 
CTAGAGGAGG TACTAAAGGA ACCTGCTAGC AAGAAGTCTT CAACACCCAA GTCTTCTCAG 
AGAGTTCTCG GGGACCAGGA GTGTGTGTTC CAGGCTCAAA GCAGGTGGAA GGGAGCCGGG 
AAATTTATCC TTAAGCTAAA AGAGCAGGTT CAGGCAGCTC GAGATGATAA AAAGAAAGGC 
ATTTCCTTTG CTAATGAACT TAAGAAATTT GCCAAGTCTA GCAAGCAGTA TCGAGGATTC 
ACCTCACCTT CTCAGCTTTC AATAGCAGAA AGTGTCCAGA TGAAGGAAGA AAAATCTGTG 
GGCAGCTTCT CTTCTGAGAC GACAGACCAT CACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100917022 ( XM_023495065.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023350833.1 Sarcophilus harrisii phospholipase C epsilon 1 (PLCE1), mRNA.

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CloneID	OSa149145
Clone ID Related Accession (Same CDS sequence)	XM_023495065.1 , XM_023495065.2
Accession Version	XM_023495065.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	LOW QUALITY PROTEIN: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816463.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGAATGCTT CCTGTNATTT GAATTATTTA CAGATCAACA ACACTGTGAG AAAAGGAGAA 
GAAACAGAAC GCACTTTTGG ATCAATTTTC CATTTCTTTG GGAAGACTCT AGCCTTAGAT 
ATAATCCATG GACATATTAA TATTGGCCCA TGCTTGAAGC AATGTGTTCG AGATACTTCA 
TGTGAATACC GAGCTACCTT TCAAAGGACT TCAATTTCTC AGTATATCAC TGGTTCTCTA 
TTGGAAGCAA CTGCGTCTCT GGGTGCAAGA AGTGGCCTTT TAAATACTTT TGGAGGATCA 
GTAGGAAGAA CAATGTTGAA AGAACGTCAA CCATGTACCT CCACAGCCAG TTCAATCAAC 
TTACCTTCAA ATGTATCTGG TTTAAGTAAC GAGCTCATAG AACTTCAACA CCTCATTCAG 
TTCCCTGAAG AAGTGGCTTG TATTCTGACT GAACAGGAGC AGAATATCTA CCGACAAGTG 
CTACCTGTCG ACTACCTTTG CTTCTTAACC AGGGACTTGG GAAATGCTGA ATGCCAGAGT 
AAACTTCCAT GCCTCAGAGC TACAGTATCT GCATCTATTC TTACCCCCCA GAACAATGAG 
CACAGTGCTC TGGAAAACCT TGTAACAAGA TTTAATGAGG TGAGTTCTTG GGTGACGTGG 
CTGATACTCA CAGCAGGTAC CATGGAAGAA AAACGGGAAG TCTTCTCATA TTTAGTACAC 
ATTGCAAAAT GCTGCTGGAA TATGGGCAAC TACAATGCTG TTATGGAATT CTTGGCTGGC 
CTCAGGTCAA GGAAAGTTTT AAAGATGTGG CAGTTTATGG ACCAGTCTGA TATTGAAACC 
ATGAGAAGCC TGAAGGATGC AATGGCCCAA CATGAATCTT CCTCTGAATA CAAGAAAGTG 
GTAAACCGGG CTCTGAATAT CCCTGGCTGT AAAGTGGTTC CTTTCTGTGG TGTCTTTTTA 
AAGGAGCTCT GTGAAATTCT AGATGGAACA TCTGGATTTA TGAAACTTTG CCCACGATAT 
AATTCACAAG ATGAAACTTT AGAGTTTGTA GCTGATTACA ATGGACAAGA TAATTTTTTA 
CAACGAGTAG GACAAGATGG CTTAAACAAC CCAGAGAAAG AATCCACTGT CAACAGTATT 
TTTCAAATTA TCCGAAGCTG CAATCGTAGT CTGGAACCTG AAGAAGAAGA CAGCTTAAGT 
GAAGGCGATG ATTCAAGGAA AAATTCCTTG AAAGATAAAA CCCGACTACA GTTTGTAATT 
GGAGAACTGT CTGATTCAGA AAGTGATATT TTTGAGCAGG CCAAAGAATG GGATTTCCAT 
GGAACGGAAG AAGAGCAGAA AGCATTCAAG CATGGCACAG AACTCATTCC CTGGTATGTC 
TTGTCAATTA GAGCTGATGT CCATCAGTTC ATACTCCAAG GAGCCACTGT TATTCACTAT 
GACCAGGATG CACATCTGTC AGCACGATGC TTTCTTCGAC TTCAACCTGA TAATAGTACT 
TTGACCTGGA TCAAACCTAC AACTGCCTCA CTCGCCAATA CTAAAGCCAA ATTAGGCATC 
ACAGGAAATT TAGCTGACCC TGGCAAACTT CAATCACTTG GCAATACTGG ATTAAATGGA 
CTGATGGAAG GTTTTCTGGA TTTGTTTTCA GTCAAGGCTG TGTACATGGG ACATCCTGGC 
ATTGATATGC ACACTGTGTG TGTTCAGAAC AAACTCAGTA ACATGTCCCT CTTAGAGAAT 
AGTGTGACAC TGCTTTATGG CCTTCAAACT ACTGAGAATA GATTATTACA CTTTGTGGCA 
CCAAAACATA CTGCTAAAAT GTTGTATAAT GGATTATTAG AATTAACAAA AGCTGTAAGA 
AAAATGAAGA AATTTCCTGA CCAGCGACAG CAGTGGCTAA GGAAGCAATA TGTCAGCCTC 
TATCAGGAGG ATGGAAGATT TGAAGGTCCA ACTTTGGCTC ATGCTATCGA ACTGTTTGGT 
GGCAGACGTT GGACTACACG AAATACAAGT CCAGCAACAC TTTCTAAAAG TGTGGAGAAA 
CCCATTGTGC AAAGGAACAA CACCCTTGGG ATAAGTGCAA ATAAGAAAAA GAAAAAAATC 
CTTATGAGGG GTGAAAGTGG AGAGGGTACT GATGATGAGA TGGCCACACG AAAGGCAAAA 
ATGCATAAGG AATGCCGTGG CCGCAGTGGC TCAGATCCTC AAGACCTTAA TGAGCAAGAA 
GAATCAGATC AGAATGTTAT TATTAGTCCC TGTAATAATC TGCCATCCAG GAGAGCCAAT 
TCTTTGACTA CAGCTGGATC TCCTAACTTG GCTGCTGGAG CAACTTCACC AGTCAGACCA 
CTTTCCTCTC CTGTATTGTC ACCTTCAAAT AAGAATCAGC CTAATACATG GAGCAGCAGT 
AGTTGGCATG GCCGAGTTAA AGGTGGACTG AAGGGATTTC AAAATTTCAT GGTTTCAGAT 
AGTAACATGA GTTTTGTTGA ATTTGTAGAG CTGTTCAAGT CATTCAGTGT ACGAAGTCGA 
AAGGACCTCA AGGATCTTTT TGACATCTAT GCTGTACCCT GCAGTCGATC AGGATCTGAG 
TCTGTCCCAC TCTATACAAA CCTGAGAATT GATGAAAATA CCAGTGGCCT TCAGCCTGAT 
TTAGATTTGT TAACCAGGAA CGTGTCTGAC TTGGGCTTGT TCATTAAGAA TAGGCAACAG 
TTATCAGACA ACCAGAGGCA GATATCTGAT GCCATCGCAG CTGCTAGTAT TGTGACTAAT 
GGTTCTGGAA TAGAAAGCAC CACGCTTGGT GTCTGTGGGG TTGGCATATT ACAGCTGAAT 
GACTTTTTAG TGAATTGCCA AGGGGAACAC TACACTTATG ATGAGATCCT CAGCATTATC 
CAGAAATTCG AGCCTAGTGT GAGTATGTAT CAGCAAGGTC TAATGTCATT TGAAGGATTT 
TCCAGGTTTC TGATGGATAA AGATAATTTT GCTTCCCAAA ATGATGAATC ACAGGAGAAT 
GTTAAAGAAT TGCAGTTACC ACTCTCCTAT TATTACATTG AATCTTCACA CAACACATAC 
CTCACAGGAC ATCAGCTTAA AGGAGAGTCC TCAGTGGAGC TATATAGCCA GGTCCTCTTG 
CAAGGCTGTC GGAGTGTGGA ATTAGACTGT TGGGATGGTG ATGATGGAAT GCCAATTATC 
TATCATGGAC ATACACTTAC TACCAAGATT CCCTTCAAGG AAGTAGTTGA AGCCATTGAT 
CGCAGTGCCT TCATCAACTC TGACTTGCCT ATTATAATAT CTATAGAAAA TCACTGTTCA 
TTGCCTCAGC AACGAAAAAT GGCAGAAATT TTCAAGAATG TATTTGGAGA AAAACTAGTT 
GCCAAATTTT TGTTTGAGAG TGATTTCTCT GATGATCCAA TGCTTCCTTC ACCTGACCAA 
CTTAGGAGAA AAGTACTTCT TAAAAACAAA AAACTGAAGG CACATCAGAC TCCAGTGGAC 
ATATTAAAGC AAAAGGCTCA TCAACTGGCA TCCATGCAAA CACAAAGCAA TAATGGTGGT 
AATGCCAATC CTATGTCTGC CAACAATGAA GAAGAAGAGG AGGAGGAGGA TGAATATGAT 
TATGACTATG AATCTCTTTC TGATGACAAC ATCCTGGAAG ACCGGCCTGA AAGTAAGTCA 
GCAAGTGACA AACTACAGTT TGAGTACAAT GAAGAAATTC CCAAGAGAAT CAAGAAGGCT 
GACAGCTCTG TTTGCAACAA GGGAAAGGTT TATGACATGG AACTGGGTGA AGAATTTTAT 
CTTCCACAGA ATAAAAAAGA GAGTAGACAG ATAGCTCCAG AGCTTTCTGA CCTTGTCATC 
TACTGCCAAG CTGTAAAATT TCCAGGATTG TCAACTTTAA ACCCATCTGG CTCTAACAGA 
GGAAAAGAAA GGAAAAGCAG GAAGTCCATT TTTGGCAACA ATCCGGGCAG ATTAAGCCCT 
GGGGAGACAG CAGCATATAC CAAAACATCT GGAAAAAGTC CCTTGGACGG CGTGCGGCAG 
ACCTGGGAAG AAGTCTGTTC TCTCAGCACA GCTCCGTCCC TCAGTGCCAT CATCAGGACC 
CCCAGGTGCT ACCACATCTC CTCCCTGAAC GAGAATGCGG CCAAGCGGCT GTGCCGCCGG 
TACTCTCGGA AGCTGATTCA GCACACCGCC TGCCAGCTGC TGAGAACCTA CCCTGCCGCC 
ACTCGCATTG ACTCCTCCAA CCCCAATCCC CTCCTGTTCT GGCTGCACGG GATACAGCTG 
GTGGCACTCA ACTACCAAAC AGACGATCTC CCCTTACACC TAAACACAGC AATGTTTGAA 
GCTAATGGTG GCTGTGGCTA TGTTTTGAAA CCCCCTGTTT TATGGGACAA GAATTGTGCT 
ATGTACCAGC AGTTTTCTCC ACTGGAAAGA GATATGGAAA ACATGGAACC TGCTCTCTAT 
TCTTTAACTA TTGTCTCTGG TCAGAATGTG TGTCCTAGTA ACAGTACAGG AAGTCCATGT 
ATTGAAATCG ATGTCCTGGG AATGCCTCTG GATAGCTGCC ATTTCCGCAC TAAACCCATT 
CATCGAAATA CTCTTAACCC CATGTGGAAC GAACAGTTTC TCTTCCGAGT TCATTTTGAA 
GATCTTGTAT TTCTTCGTTT TGCGGTTGTT GAAAACAACA GTTCAGCTGT AACTGCCCAA 
AGAATCATTC CACTGAAGGC TTTAAAAAGA GGATATCGGC ATCTTCAGCT GAGAAACCTC 
CATAATGAAG TCTTAGAAAT CTCTAGCTTG TTCATAAACA GCAGGAGGAT GGAAGAGCAT 
TCCAGTGGCA GTGCTGTGGC AGCATCTTTG ATGTTCAGCA CAGAAGAAAG AAAAGGTCTG 
ATGACATACA AGGTCACAGT ACATGGGATT CCAGGTCCTG AGCCTTTTGC CATTTTCATT 
ATAAATAAAG AAATTAAAGC AAAGCAGCTT CTTCACCAAA TTTTGACTGC TTCACAAAAC 
TCCATACCAG TCACTCCGGA CTGTTTTTTA ATGGAAGAAA AATATTTTAT ATCTAAAGAA 
AAAAATGACT TCAGGAAACA ACCATTCCAG AGAATAATCG GTCCAGAAGA AGAGATTGTG 
CAGATTTTGA ACAGCTGGTT CCCAGAAGAA GGTTATGTTG GAAGGATTAT TTTTAAAATC 
CAGGAGGAAC GCCCAGAAGA GAAGAACATC TTCCAAGAAG AGAAGGAGGT GATGCTGAGC 
TCAGAAGATG AAAGCTTCTT TGTACAAGTC CATGATGTTT CTCCCGAGCA GCCCCGAACA 
GTCATCAAAG CTCCCCGAGT CAGCACTGCC CAGGAGGTCA TCCAGCAGAC TCTATGCAAA 
GCAAAATATT CTTACAGCAT TTTGAGCAAT CCCAACCCGA GTGACTACGT GCTCCTAGAG 
GAGGTACTAA AGGAACCTGC TAGCAAGAAG TCTTCAACAC CCAAGTCTTC TCAGAGAGTT 
CTCGGGGACC AGGAGTGTGT GTTCCAGGCT CAAAGCAGGT GGAAGGGAGC CGGGAAATTT 
ATCCTTAAGC TAAAAGAGCA GGTTCAGGCA GCTCGAGATG ATAAAAAGAA AGGCATTTCC 
TTTGCTAATG AACTTAAGAA ATTTGCCAAG TCTAGCAAGC AGTATCGAGG ATTCACCTCA 
CCTTCTCAGC TTTCAATAGC AGAAAGTGTC CAGATGAAGG AAGAAAAATC TGTGGGCAGC 
TTCTCTTCTG AGACGACAGA CCATCACCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023350833.1
CDS	1..5733
Translation	MNASCnullLNYLQINNTVRKGEETERTFGSIFHFFGKTLALDIIHGHINIGPCLKQCVRDTSCEYRATFQRTSISQYITGSLLEATASLGARSGLLNTFGGSVGRTMLKERQPCTSTASSINLPSNVSGLSNELIELQHLIQFPEEVACILTEQEQNIYRQVLPVDYLCFLTRDLGNAECQSKLPCLRATVSASILTPQNNEHSALENLVTRFNEVSSWVTWLILTAGTMEEKREVFSYLVHIAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSSEYKKVVNRALNIPGCKVVPFCGVFLKELCEILDGTSGFMKLCPRYNSQDETLEFVADYNGQDNFLQRVGQDGLNNPEKESTVNSIFQIIRSCNRSLEPEEEDSLSEGDDSRKNSLKDKTRLQFVIGELSDSESDIFEQAKEWDFHGTEEEQKAFKHGTELIPWYVLSIRADVHQFILQGATVIHYDQDAHLSARCFLRLQPDNSTLTWIKPTTASLANTKAKLGITGNLADPGKLQSLGNTGLNGLMEGFLDLFSVKAVYMGHPGIDMHTVCVQNKLSNMSLLENSVTLLYGLQTTENRLLHFVAPKHTAKMLYNGLLELTKAVRKMKKFPDQRQQWLRKQYVSLYQEDGRFEGPTLAHAIELFGGRRWTTRNTSPATLSKSVEKPIVQRNNTLGISANKKKKKILMRGESGEGTDDEMATRKAKMHKECRGRSGSDPQDLNEQEESDQNVIISPCNNLPSRRANSLTTAGSPNLAAGATSPVRPLSSPVLSPSNKNQPNTWSSSSWHGRVKGGLKGFQNFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDIYAVPCSRSGSESVPLYTNLRIDENTSGLQPDLDLLTRNVSDLGLFIKNRQQLSDNQRQISDAIAAASIVTNGSGIESTTLGVCGVGILQLNDFLVNCQGEHYTYDEILSIIQKFEPSVSMYQQGLMSFEGFSRFLMDKDNFASQNDESQENVKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKNVFGEKLVAKFLFESDFSDDPMLPSPDQLRRKVLLKNKKLKAHQTPVDILKQKAHQLASMQTQSNNGGNANPMSANNEEEEEEEDEYDYDYESLSDDNILEDRPESKSASDKLQFEYNEEIPKRIKKADSSVCNKGKVYDMELGEEFYLPQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNPSGSNRGKERKSRKSIFGNNPGRLSPGETAAYTKTSGKSPLDGVRQTWEEVCSLSTAPSLSAIIRTPRCYHISSLNENAAKRLCRRYSRKLIQHTACQLLRTYPAATRIDSSNPNPLLFWLHGIQLVALNYQTDDLPLHLNTAMFEANGGCGYVLKPPVLWDKNCAMYQQFSPLERDMENMEPALYSLTIVSGQNVCPSNSTGSPCIEIDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFRVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEEHSSGSAVAASLMFSTEERKGLMTYKVTVHGIPGPEPFAIFIINKEIKAKQLLHQILTASQNSIPVTPDCFLMEEKYFISKEKNDFRKQPFQRIIGPEEEIVQILNSWFPEEGYVGRIIFKIQEERPEEKNIFQEEKEVMLSSEDESFFVQVHDVSPEQPRTVIKAPRVSTAQEVIQQTLCKAKYSYSILSNPNPSDYVLLEEVLKEPASKKSSTPKSSQRVLGDQECVFQAQSRWKGAGKFILKLKEQVQAARDDKKKGISFANELKKFAKSSKQYRGFTSPSQLSIAESVQMKEEKSVGSFSSETTDHHP

Target ORF information:

RefSeq Version	XM_023495065.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii phospholipase C epsilon 1 (PLCE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023495065.1

ORF Insert Sequence:

ATGAATGCTT CCTGTNATTT GAATTATTTA CAGATCAACA ACACTGTGAG AAAAGGAGAA 
GAAACAGAAC GCACTTTTGG ATCAATTTTC CATTTCTTTG GGAAGACTCT AGCCTTAGAT 
ATAATCCATG GACATATTAA TATTGGCCCA TGCTTGAAGC AATGTGTTCG AGATACTTCA 
TGTGAATACC GAGCTACCTT TCAAAGGACT TCAATTTCTC AGTATATCAC TGGTTCTCTA 
TTGGAAGCAA CTGCGTCTCT GGGTGCAAGA AGTGGCCTTT TAAATACTTT TGGAGGATCA 
GTAGGAAGAA CAATGTTGAA AGAACGTCAA CCATGTACCT CCACAGCCAG TTCAATCAAC 
TTACCTTCAA ATGTATCTGG TTTAAGTAAC GAGCTCATAG AACTTCAACA CCTCATTCAG 
TTCCCTGAAG AAGTGGCTTG TATTCTGACT GAACAGGAGC AGAATATCTA CCGACAAGTG 
CTACCTGTCG ACTACCTTTG CTTCTTAACC AGGGACTTGG GAAATGCTGA ATGCCAGAGT 
AAACTTCCAT GCCTCAGAGC TACAGTATCT GCATCTATTC TTACCCCCCA GAACAATGAG 
CACAGTGCTC TGGAAAACCT TGTAACAAGA TTTAATGAGG TGAGTTCTTG GGTGACGTGG 
CTGATACTCA CAGCAGGTAC CATGGAAGAA AAACGGGAAG TCTTCTCATA TTTAGTACAC 
ATTGCAAAAT GCTGCTGGAA TATGGGCAAC TACAATGCTG TTATGGAATT CTTGGCTGGC 
CTCAGGTCAA GGAAAGTTTT AAAGATGTGG CAGTTTATGG ACCAGTCTGA TATTGAAACC 
ATGAGAAGCC TGAAGGATGC AATGGCCCAA CATGAATCTT CCTCTGAATA CAAGAAAGTG 
GTAAACCGGG CTCTGAATAT CCCTGGCTGT AAAGTGGTTC CTTTCTGTGG TGTCTTTTTA 
AAGGAGCTCT GTGAAATTCT AGATGGAACA TCTGGATTTA TGAAACTTTG CCCACGATAT 
AATTCACAAG ATGAAACTTT AGAGTTTGTA GCTGATTACA ATGGACAAGA TAATTTTTTA 
CAACGAGTAG GACAAGATGG CTTAAACAAC CCAGAGAAAG AATCCACTGT CAACAGTATT 
TTTCAAATTA TCCGAAGCTG CAATCGTAGT CTGGAACCTG AAGAAGAAGA CAGCTTAAGT 
GAAGGCGATG ATTCAAGGAA AAATTCCTTG AAAGATAAAA CCCGACTACA GTTTGTAATT 
GGAGAACTGT CTGATTCAGA AAGTGATATT TTTGAGCAGG CCAAAGAATG GGATTTCCAT 
GGAACGGAAG AAGAGCAGAA AGCATTCAAG CATGGCACAG AACTCATTCC CTGGTATGTC 
TTGTCAATTA GAGCTGATGT CCATCAGTTC ATACTCCAAG GAGCCACTGT TATTCACTAT 
GACCAGGATG CACATCTGTC AGCACGATGC TTTCTTCGAC TTCAACCTGA TAATAGTACT 
TTGACCTGGA TCAAACCTAC AACTGCCTCA CTCGCCAATA CTAAAGCCAA ATTAGGCATC 
ACAGGAAATT TAGCTGACCC TGGCAAACTT CAATCACTTG GCAATACTGG ATTAAATGGA 
CTGATGGAAG GTTTTCTGGA TTTGTTTTCA GTCAAGGCTG TGTACATGGG ACATCCTGGC 
ATTGATATGC ACACTGTGTG TGTTCAGAAC AAACTCAGTA ACATGTCCCT CTTAGAGAAT 
AGTGTGACAC TGCTTTATGG CCTTCAAACT ACTGAGAATA GATTATTACA CTTTGTGGCA 
CCAAAACATA CTGCTAAAAT GTTGTATAAT GGATTATTAG AATTAACAAA AGCTGTAAGA 
AAAATGAAGA AATTTCCTGA CCAGCGACAG CAGTGGCTAA GGAAGCAATA TGTCAGCCTC 
TATCAGGAGG ATGGAAGATT TGAAGGTCCA ACTTTGGCTC ATGCTATCGA ACTGTTTGGT 
GGCAGACGTT GGACTACACG AAATACAAGT CCAGCAACAC TTTCTAAAAG TGTGGAGAAA 
CCCATTGTGC AAAGGAACAA CACCCTTGGG ATAAGTGCAA ATAAGAAAAA GAAAAAAATC 
CTTATGAGGG GTGAAAGTGG AGAGGGTACT GATGATGAGA TGGCCACACG AAAGGCAAAA 
ATGCATAAGG AATGCCGTGG CCGCAGTGGC TCAGATCCTC AAGACCTTAA TGAGCAAGAA 
GAATCAGATC AGAATGTTAT TATTAGTCCC TGTAATAATC TGCCATCCAG GAGAGCCAAT 
TCTTTGACTA CAGCTGGATC TCCTAACTTG GCTGCTGGAG CAACTTCACC AGTCAGACCA 
CTTTCCTCTC CTGTATTGTC ACCTTCAAAT AAGAATCAGC CTAATACATG GAGCAGCAGT 
AGTTGGCATG GCCGAGTTAA AGGTGGACTG AAGGGATTTC AAAATTTCAT GGTTTCAGAT 
AGTAACATGA GTTTTGTTGA ATTTGTAGAG CTGTTCAAGT CATTCAGTGT ACGAAGTCGA 
AAGGACCTCA AGGATCTTTT TGACATCTAT GCTGTACCCT GCAGTCGATC AGGATCTGAG 
TCTGTCCCAC TCTATACAAA CCTGAGAATT GATGAAAATA CCAGTGGCCT TCAGCCTGAT 
TTAGATTTGT TAACCAGGAA CGTGTCTGAC TTGGGCTTGT TCATTAAGAA TAGGCAACAG 
TTATCAGACA ACCAGAGGCA GATATCTGAT GCCATCGCAG CTGCTAGTAT TGTGACTAAT 
GGTTCTGGAA TAGAAAGCAC CACGCTTGGT GTCTGTGGGG TTGGCATATT ACAGCTGAAT 
GACTTTTTAG TGAATTGCCA AGGGGAACAC TACACTTATG ATGAGATCCT CAGCATTATC 
CAGAAATTCG AGCCTAGTGT GAGTATGTAT CAGCAAGGTC TAATGTCATT TGAAGGATTT 
TCCAGGTTTC TGATGGATAA AGATAATTTT GCTTCCCAAA ATGATGAATC ACAGGAGAAT 
GTTAAAGAAT TGCAGTTACC ACTCTCCTAT TATTACATTG AATCTTCACA CAACACATAC 
CTCACAGGAC ATCAGCTTAA AGGAGAGTCC TCAGTGGAGC TATATAGCCA GGTCCTCTTG 
CAAGGCTGTC GGAGTGTGGA ATTAGACTGT TGGGATGGTG ATGATGGAAT GCCAATTATC 
TATCATGGAC ATACACTTAC TACCAAGATT CCCTTCAAGG AAGTAGTTGA AGCCATTGAT 
CGCAGTGCCT TCATCAACTC TGACTTGCCT ATTATAATAT CTATAGAAAA TCACTGTTCA 
TTGCCTCAGC AACGAAAAAT GGCAGAAATT TTCAAGAATG TATTTGGAGA AAAACTAGTT 
GCCAAATTTT TGTTTGAGAG TGATTTCTCT GATGATCCAA TGCTTCCTTC ACCTGACCAA 
CTTAGGAGAA AAGTACTTCT TAAAAACAAA AAACTGAAGG CACATCAGAC TCCAGTGGAC 
ATATTAAAGC AAAAGGCTCA TCAACTGGCA TCCATGCAAA CACAAAGCAA TAATGGTGGT 
AATGCCAATC CTATGTCTGC CAACAATGAA GAAGAAGAGG AGGAGGAGGA TGAATATGAT 
TATGACTATG AATCTCTTTC TGATGACAAC ATCCTGGAAG ACCGGCCTGA AAGTAAGTCA 
GCAAGTGACA AACTACAGTT TGAGTACAAT GAAGAAATTC CCAAGAGAAT CAAGAAGGCT 
GACAGCTCTG TTTGCAACAA GGGAAAGGTT TATGACATGG AACTGGGTGA AGAATTTTAT 
CTTCCACAGA ATAAAAAAGA GAGTAGACAG ATAGCTCCAG AGCTTTCTGA CCTTGTCATC 
TACTGCCAAG CTGTAAAATT TCCAGGATTG TCAACTTTAA ACCCATCTGG CTCTAACAGA 
GGAAAAGAAA GGAAAAGCAG GAAGTCCATT TTTGGCAACA ATCCGGGCAG ATTAAGCCCT 
GGGGAGACAG CAGCATATAC CAAAACATCT GGAAAAAGTC CCTTGGACGG CGTGCGGCAG 
ACCTGGGAAG AAGTCTGTTC TCTCAGCACA GCTCCGTCCC TCAGTGCCAT CATCAGGACC 
CCCAGGTGCT ACCACATCTC CTCCCTGAAC GAGAATGCGG CCAAGCGGCT GTGCCGCCGG 
TACTCTCGGA AGCTGATTCA GCACACCGCC TGCCAGCTGC TGAGAACCTA CCCTGCCGCC 
ACTCGCATTG ACTCCTCCAA CCCCAATCCC CTCCTGTTCT GGCTGCACGG GATACAGCTG 
GTGGCACTCA ACTACCAAAC AGACGATCTC CCCTTACACC TAAACACAGC AATGTTTGAA 
GCTAATGGTG GCTGTGGCTA TGTTTTGAAA CCCCCTGTTT TATGGGACAA GAATTGTGCT 
ATGTACCAGC AGTTTTCTCC ACTGGAAAGA GATATGGAAA ACATGGAACC TGCTCTCTAT 
TCTTTAACTA TTGTCTCTGG TCAGAATGTG TGTCCTAGTA ACAGTACAGG AAGTCCATGT 
ATTGAAATCG ATGTCCTGGG AATGCCTCTG GATAGCTGCC ATTTCCGCAC TAAACCCATT 
CATCGAAATA CTCTTAACCC CATGTGGAAC GAACAGTTTC TCTTCCGAGT TCATTTTGAA 
GATCTTGTAT TTCTTCGTTT TGCGGTTGTT GAAAACAACA GTTCAGCTGT AACTGCCCAA 
AGAATCATTC CACTGAAGGC TTTAAAAAGA GGATATCGGC ATCTTCAGCT GAGAAACCTC 
CATAATGAAG TCTTAGAAAT CTCTAGCTTG TTCATAAACA GCAGGAGGAT GGAAGAGCAT 
TCCAGTGGCA GTGCTGTGGC AGCATCTTTG ATGTTCAGCA CAGAAGAAAG AAAAGGTCTG 
ATGACATACA AGGTCACAGT ACATGGGATT CCAGGTCCTG AGCCTTTTGC CATTTTCATT 
ATAAATAAAG AAATTAAAGC AAAGCAGCTT CTTCACCAAA TTTTGACTGC TTCACAAAAC 
TCCATACCAG TCACTCCGGA CTGTTTTTTA ATGGAAGAAA AATATTTTAT ATCTAAAGAA 
AAAAATGACT TCAGGAAACA ACCATTCCAG AGAATAATCG GTCCAGAAGA AGAGATTGTG 
CAGATTTTGA ACAGCTGGTT CCCAGAAGAA GGTTATGTTG GAAGGATTAT TTTTAAAATC 
CAGGAGGAAC GCCCAGAAGA GAAGAACATC TTCCAAGAAG AGAAGGAGGT GATGCTGAGC 
TCAGAAGATG AAAGCTTCTT TGTACAAGTC CATGATGTTT CTCCCGAGCA GCCCCGAACA 
GTCATCAAAG CTCCCCGAGT CAGCACTGCC CAGGAGGTCA TCCAGCAGAC TCTATGCAAA 
GCAAAATATT CTTACAGCAT TTTGAGCAAT CCCAACCCGA GTGACTACGT GCTCCTAGAG 
GAGGTACTAA AGGAACCTGC TAGCAAGAAG TCTTCAACAC CCAAGTCTTC TCAGAGAGTT 
CTCGGGGACC AGGAGTGTGT GTTCCAGGCT CAAAGCAGGT GGAAGGGAGC CGGGAAATTT 
ATCCTTAAGC TAAAAGAGCA GGTTCAGGCA GCTCGAGATG ATAAAAAGAA AGGCATTTCC 
TTTGCTAATG AACTTAAGAA ATTTGCCAAG TCTAGCAAGC AGTATCGAGG ATTCACCTCA 
CCTTCTCAGC TTTCAATAGC AGAAAGTGTC CAGATGAAGG AAGAAAAATC TGTGGGCAGC 
TTCTCTTCTG AGACGACAGA CCATCACCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100917022 ( XM_023495065.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023350833.1 Sarcophilus harrisii 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (LOC100917022), mRNA.

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CloneID	OSa149145
Clone ID Related Accession (Same CDS sequence)	XM_023495065.1 , XM_023495065.2
Accession Version	XM_023495065.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	LOW QUALITY PROTEIN: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 31, 2019 this sequence version replaced XM_023495065.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGAATGCTT CCTGTNATTT GAATTATTTA CAGATCAACA ACACTGTGAG AAAAGGAGAA 
GAAACAGAAC GCACTTTTGG ATCAATTTTC CATTTCTTTG GGAAGACTCT AGCCTTAGAT 
ATAATCCATG GACATATTAA TATTGGCCCA TGCTTGAAGC AATGTGTTCG AGATACTTCA 
TGTGAATACC GAGCTACCTT TCAAAGGACT TCAATTTCTC AGTATATCAC TGGTTCTCTA 
TTGGAAGCAA CTGCGTCTCT GGGTGCAAGA AGTGGCCTTT TAAATACTTT TGGAGGATCA 
GTAGGAAGAA CAATGTTGAA AGAACGTCAA CCATGTACCT CCACAGCCAG TTCAATCAAC 
TTACCTTCAA ATGTATCTGG TTTAAGTAAC GAGCTCATAG AACTTCAACA CCTCATTCAG 
TTCCCTGAAG AAGTGGCTTG TATTCTGACT GAACAGGAGC AGAATATCTA CCGACAAGTG 
CTACCTGTCG ACTACCTTTG CTTCTTAACC AGGGACTTGG GAAATGCTGA ATGCCAGAGT 
AAACTTCCAT GCCTCAGAGC TACAGTATCT GCATCTATTC TTACCCCCCA GAACAATGAG 
CACAGTGCTC TGGAAAACCT TGTAACAAGA TTTAATGAGG TGAGTTCTTG GGTGACGTGG 
CTGATACTCA CAGCAGGTAC CATGGAAGAA AAACGGGAAG TCTTCTCATA TTTAGTACAC 
ATTGCAAAAT GCTGCTGGAA TATGGGCAAC TACAATGCTG TTATGGAATT CTTGGCTGGC 
CTCAGGTCAA GGAAAGTTTT AAAGATGTGG CAGTTTATGG ACCAGTCTGA TATTGAAACC 
ATGAGAAGCC TGAAGGATGC AATGGCCCAA CATGAATCTT CCTCTGAATA CAAGAAAGTG 
GTAAACCGGG CTCTGAATAT CCCTGGCTGT AAAGTGGTTC CTTTCTGTGG TGTCTTTTTA 
AAGGAGCTCT GTGAAATTCT AGATGGAACA TCTGGATTTA TGAAACTTTG CCCACGATAT 
AATTCACAAG ATGAAACTTT AGAGTTTGTA GCTGATTACA ATGGACAAGA TAATTTTTTA 
CAACGAGTAG GACAAGATGG CTTAAACAAC CCAGAGAAAG AATCCACTGT CAACAGTATT 
TTTCAAATTA TCCGAAGCTG CAATCGTAGT CTGGAACCTG AAGAAGAAGA CAGCTTAAGT 
GAAGGCGATG ATTCAAGGAA AAATTCCTTG AAAGATAAAA CCCGACTACA GTTTGTAATT 
GGAGAACTGT CTGATTCAGA AAGTGATATT TTTGAGCAGG CCAAAGAATG GGATTTCCAT 
GGAACGGAAG AAGAGCAGAA AGCATTCAAG CATGGCACAG AACTCATTCC CTGGTATGTC 
TTGTCAATTA GAGCTGATGT CCATCAGTTC ATACTCCAAG GAGCCACTGT TATTCACTAT 
GACCAGGATG CACATCTGTC AGCACGATGC TTTCTTCGAC TTCAACCTGA TAATAGTACT 
TTGACCTGGA TCAAACCTAC AACTGCCTCA CTCGCCAATA CTAAAGCCAA ATTAGGCATC 
ACAGGAAATT TAGCTGACCC TGGCAAACTT CAATCACTTG GCAATACTGG ATTAAATGGA 
CTGATGGAAG GTTTTCTGGA TTTGTTTTCA GTCAAGGCTG TGTACATGGG ACATCCTGGC 
ATTGATATGC ACACTGTGTG TGTTCAGAAC AAACTCAGTA ACATGTCCCT CTTAGAGAAT 
AGTGTGACAC TGCTTTATGG CCTTCAAACT ACTGAGAATA GATTATTACA CTTTGTGGCA 
CCAAAACATA CTGCTAAAAT GTTGTATAAT GGATTATTAG AATTAACAAA AGCTGTAAGA 
AAAATGAAGA AATTTCCTGA CCAGCGACAG CAGTGGCTAA GGAAGCAATA TGTCAGCCTC 
TATCAGGAGG ATGGAAGATT TGAAGGTCCA ACTTTGGCTC ATGCTATCGA ACTGTTTGGT 
GGCAGACGTT GGACTACACG AAATACAAGT CCAGCAACAC TTTCTAAAAG TGTGGAGAAA 
CCCATTGTGC AAAGGAACAA CACCCTTGGG ATAAGTGCAA ATAAGAAAAA GAAAAAAATC 
CTTATGAGGG GTGAAAGTGG AGAGGGTACT GATGATGAGA TGGCCACACG AAAGGCAAAA 
ATGCATAAGG AATGCCGTGG CCGCAGTGGC TCAGATCCTC AAGACCTTAA TGAGCAAGAA 
GAATCAGATC AGAATGTTAT TATTAGTCCC TGTAATAATC TGCCATCCAG GAGAGCCAAT 
TCTTTGACTA CAGCTGGATC TCCTAACTTG GCTGCTGGAG CAACTTCACC AGTCAGACCA 
CTTTCCTCTC CTGTATTGTC ACCTTCAAAT AAGAATCAGC CTAATACATG GAGCAGCAGT 
AGTTGGCATG GCCGAGTTAA AGGTGGACTG AAGGGATTTC AAAATTTCAT GGTTTCAGAT 
AGTAACATGA GTTTTGTTGA ATTTGTAGAG CTGTTCAAGT CATTCAGTGT ACGAAGTCGA 
AAGGACCTCA AGGATCTTTT TGACATCTAT GCTGTACCCT GCAGTCGATC AGGATCTGAG 
TCTGTCCCAC TCTATACAAA CCTGAGAATT GATGAAAATA CCAGTGGCCT TCAGCCTGAT 
TTAGATTTGT TAACCAGGAA CGTGTCTGAC TTGGGCTTGT TCATTAAGAA TAGGCAACAG 
TTATCAGACA ACCAGAGGCA GATATCTGAT GCCATCGCAG CTGCTAGTAT TGTGACTAAT 
GGTTCTGGAA TAGAAAGCAC CACGCTTGGT GTCTGTGGGG TTGGCATATT ACAGCTGAAT 
GACTTTTTAG TGAATTGCCA AGGGGAACAC TACACTTATG ATGAGATCCT CAGCATTATC 
CAGAAATTCG AGCCTAGTGT GAGTATGTAT CAGCAAGGTC TAATGTCATT TGAAGGATTT 
TCCAGGTTTC TGATGGATAA AGATAATTTT GCTTCCCAAA ATGATGAATC ACAGGAGAAT 
GTTAAAGAAT TGCAGTTACC ACTCTCCTAT TATTACATTG AATCTTCACA CAACACATAC 
CTCACAGGAC ATCAGCTTAA AGGAGAGTCC TCAGTGGAGC TATATAGCCA GGTCCTCTTG 
CAAGGCTGTC GGAGTGTGGA ATTAGACTGT TGGGATGGTG ATGATGGAAT GCCAATTATC 
TATCATGGAC ATACACTTAC TACCAAGATT CCCTTCAAGG AAGTAGTTGA AGCCATTGAT 
CGCAGTGCCT TCATCAACTC TGACTTGCCT ATTATAATAT CTATAGAAAA TCACTGTTCA 
TTGCCTCAGC AACGAAAAAT GGCAGAAATT TTCAAGAATG TATTTGGAGA AAAACTAGTT 
GCCAAATTTT TGTTTGAGAG TGATTTCTCT GATGATCCAA TGCTTCCTTC ACCTGACCAA 
CTTAGGAGAA AAGTACTTCT TAAAAACAAA AAACTGAAGG CACATCAGAC TCCAGTGGAC 
ATATTAAAGC AAAAGGCTCA TCAACTGGCA TCCATGCAAA CACAAAGCAA TAATGGTGGT 
AATGCCAATC CTATGTCTGC CAACAATGAA GAAGAAGAGG AGGAGGAGGA TGAATATGAT 
TATGACTATG AATCTCTTTC TGATGACAAC ATCCTGGAAG ACCGGCCTGA AAGTAAGTCA 
GCAAGTGACA AACTACAGTT TGAGTACAAT GAAGAAATTC CCAAGAGAAT CAAGAAGGCT 
GACAGCTCTG TTTGCAACAA GGGAAAGGTT TATGACATGG AACTGGGTGA AGAATTTTAT 
CTTCCACAGA ATAAAAAAGA GAGTAGACAG ATAGCTCCAG AGCTTTCTGA CCTTGTCATC 
TACTGCCAAG CTGTAAAATT TCCAGGATTG TCAACTTTAA ACCCATCTGG CTCTAACAGA 
GGAAAAGAAA GGAAAAGCAG GAAGTCCATT TTTGGCAACA ATCCGGGCAG ATTAAGCCCT 
GGGGAGACAG CAGCATATAC CAAAACATCT GGAAAAAGTC CCTTGGACGG CGTGCGGCAG 
ACCTGGGAAG AAGTCTGTTC TCTCAGCACA GCTCCGTCCC TCAGTGCCAT CATCAGGACC 
CCCAGGTGCT ACCACATCTC CTCCCTGAAC GAGAATGCGG CCAAGCGGCT GTGCCGCCGG 
TACTCTCGGA AGCTGATTCA GCACACCGCC TGCCAGCTGC TGAGAACCTA CCCTGCCGCC 
ACTCGCATTG ACTCCTCCAA CCCCAATCCC CTCCTGTTCT GGCTGCACGG GATACAGCTG 
GTGGCACTCA ACTACCAAAC AGACGATCTC CCCTTACACC TAAACACAGC AATGTTTGAA 
GCTAATGGTG GCTGTGGCTA TGTTTTGAAA CCCCCTGTTT TATGGGACAA GAATTGTGCT 
ATGTACCAGC AGTTTTCTCC ACTGGAAAGA GATATGGAAA ACATGGAACC TGCTCTCTAT 
TCTTTAACTA TTGTCTCTGG TCAGAATGTG TGTCCTAGTA ACAGTACAGG AAGTCCATGT 
ATTGAAATCG ATGTCCTGGG AATGCCTCTG GATAGCTGCC ATTTCCGCAC TAAACCCATT 
CATCGAAATA CTCTTAACCC CATGTGGAAC GAACAGTTTC TCTTCCGAGT TCATTTTGAA 
GATCTTGTAT TTCTTCGTTT TGCGGTTGTT GAAAACAACA GTTCAGCTGT AACTGCCCAA 
AGAATCATTC CACTGAAGGC TTTAAAAAGA GGATATCGGC ATCTTCAGCT GAGAAACCTC 
CATAATGAAG TCTTAGAAAT CTCTAGCTTG TTCATAAACA GCAGGAGGAT GGAAGAGCAT 
TCCAGTGGCA GTGCTGTGGC AGCATCTTTG ATGTTCAGCA CAGAAGAAAG AAAAGGTCTG 
ATGACATACA AGGTCACAGT ACATGGGATT CCAGGTCCTG AGCCTTTTGC CATTTTCATT 
ATAAATAAAG AAATTAAAGC AAAGCAGCTT CTTCACCAAA TTTTGACTGC TTCACAAAAC 
TCCATACCAG TCACTCCGGA CTGTTTTTTA ATGGAAGAAA AATATTTTAT ATCTAAAGAA 
AAAAATGACT TCAGGAAACA ACCATTCCAG AGAATAATCG GTCCAGAAGA AGAGATTGTG 
CAGATTTTGA ACAGCTGGTT CCCAGAAGAA GGTTATGTTG GAAGGATTAT TTTTAAAATC 
CAGGAGGAAC GCCCAGAAGA GAAGAACATC TTCCAAGAAG AGAAGGAGGT GATGCTGAGC 
TCAGAAGATG AAAGCTTCTT TGTACAAGTC CATGATGTTT CTCCCGAGCA GCCCCGAACA 
GTCATCAAAG CTCCCCGAGT CAGCACTGCC CAGGAGGTCA TCCAGCAGAC TCTATGCAAA 
GCAAAATATT CTTACAGCAT TTTGAGCAAT CCCAACCCGA GTGACTACGT GCTCCTAGAG 
GAGGTACTAA AGGAACCTGC TAGCAAGAAG TCTTCAACAC CCAAGTCTTC TCAGAGAGTT 
CTCGGGGACC AGGAGTGTGT GTTCCAGGCT CAAAGCAGGT GGAAGGGAGC CGGGAAATTT 
ATCCTTAAGC TAAAAGAGCA GGTTCAGGCA GCTCGAGATG ATAAAAAGAA AGGCATTTCC 
TTTGCTAATG AACTTAAGAA ATTTGCCAAG TCTAGCAAGC AGTATCGAGG ATTCACCTCA 
CCTTCTCAGC TTTCAATAGC AGAAAGTGTC CAGATGAAGG AAGAAAAATC TGTGGGCAGC 
TTCTCTTCTG AGACGACAGA CCATCACCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023350833.1
CDS	16..5748
Translation	MNASCnullLNYLQINNTVRKGEETERTFGSIFHFFGKTLALDIIHGHINIGPCLKQCVRDTSCEYRATFQRTSISQYITGSLLEATASLGARSGLLNTFGGSVGRTMLKERQPCTSTASSINLPSNVSGLSNELIELQHLIQFPEEVACILTEQEQNIYRQVLPVDYLCFLTRDLGNAECQSKLPCLRATVSASILTPQNNEHSALENLVTRFNEVSSWVTWLILTAGTMEEKREVFSYLVHIAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSSEYKKVVNRALNIPGCKVVPFCGVFLKELCEILDGTSGFMKLCPRYNSQDETLEFVADYNGQDNFLQRVGQDGLNNPEKESTVNSIFQIIRSCNRSLEPEEEDSLSEGDDSRKNSLKDKTRLQFVIGELSDSESDIFEQAKEWDFHGTEEEQKAFKHGTELIPWYVLSIRADVHQFILQGATVIHYDQDAHLSARCFLRLQPDNSTLTWIKPTTASLANTKAKLGITGNLADPGKLQSLGNTGLNGLMEGFLDLFSVKAVYMGHPGIDMHTVCVQNKLSNMSLLENSVTLLYGLQTTENRLLHFVAPKHTAKMLYNGLLELTKAVRKMKKFPDQRQQWLRKQYVSLYQEDGRFEGPTLAHAIELFGGRRWTTRNTSPATLSKSVEKPIVQRNNTLGISANKKKKKILMRGESGEGTDDEMATRKAKMHKECRGRSGSDPQDLNEQEESDQNVIISPCNNLPSRRANSLTTAGSPNLAAGATSPVRPLSSPVLSPSNKNQPNTWSSSSWHGRVKGGLKGFQNFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDIYAVPCSRSGSESVPLYTNLRIDENTSGLQPDLDLLTRNVSDLGLFIKNRQQLSDNQRQISDAIAAASIVTNGSGIESTTLGVCGVGILQLNDFLVNCQGEHYTYDEILSIIQKFEPSVSMYQQGLMSFEGFSRFLMDKDNFASQNDESQENVKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKNVFGEKLVAKFLFESDFSDDPMLPSPDQLRRKVLLKNKKLKAHQTPVDILKQKAHQLASMQTQSNNGGNANPMSANNEEEEEEEDEYDYDYESLSDDNILEDRPESKSASDKLQFEYNEEIPKRIKKADSSVCNKGKVYDMELGEEFYLPQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNPSGSNRGKERKSRKSIFGNNPGRLSPGETAAYTKTSGKSPLDGVRQTWEEVCSLSTAPSLSAIIRTPRCYHISSLNENAAKRLCRRYSRKLIQHTACQLLRTYPAATRIDSSNPNPLLFWLHGIQLVALNYQTDDLPLHLNTAMFEANGGCGYVLKPPVLWDKNCAMYQQFSPLERDMENMEPALYSLTIVSGQNVCPSNSTGSPCIEIDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFRVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEEHSSGSAVAASLMFSTEERKGLMTYKVTVHGIPGPEPFAIFIINKEIKAKQLLHQILTASQNSIPVTPDCFLMEEKYFISKEKNDFRKQPFQRIIGPEEEIVQILNSWFPEEGYVGRIIFKIQEERPEEKNIFQEEKEVMLSSEDESFFVQVHDVSPEQPRTVIKAPRVSTAQEVIQQTLCKAKYSYSILSNPNPSDYVLLEEVLKEPASKKSSTPKSSQRVLGDQECVFQAQSRWKGAGKFILKLKEQVQAARDDKKKGISFANELKKFAKSSKQYRGFTSPSQLSIAESVQMKEEKSVGSFSSETTDHHP

Target ORF information:

RefSeq Version	XM_023495065.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (LOC100917022), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023495065.2

ORF Insert Sequence:

ATGAATGCTT CCTGTNATTT GAATTATTTA CAGATCAACA ACACTGTGAG AAAAGGAGAA 
GAAACAGAAC GCACTTTTGG ATCAATTTTC CATTTCTTTG GGAAGACTCT AGCCTTAGAT 
ATAATCCATG GACATATTAA TATTGGCCCA TGCTTGAAGC AATGTGTTCG AGATACTTCA 
TGTGAATACC GAGCTACCTT TCAAAGGACT TCAATTTCTC AGTATATCAC TGGTTCTCTA 
TTGGAAGCAA CTGCGTCTCT GGGTGCAAGA AGTGGCCTTT TAAATACTTT TGGAGGATCA 
GTAGGAAGAA CAATGTTGAA AGAACGTCAA CCATGTACCT CCACAGCCAG TTCAATCAAC 
TTACCTTCAA ATGTATCTGG TTTAAGTAAC GAGCTCATAG AACTTCAACA CCTCATTCAG 
TTCCCTGAAG AAGTGGCTTG TATTCTGACT GAACAGGAGC AGAATATCTA CCGACAAGTG 
CTACCTGTCG ACTACCTTTG CTTCTTAACC AGGGACTTGG GAAATGCTGA ATGCCAGAGT 
AAACTTCCAT GCCTCAGAGC TACAGTATCT GCATCTATTC TTACCCCCCA GAACAATGAG 
CACAGTGCTC TGGAAAACCT TGTAACAAGA TTTAATGAGG TGAGTTCTTG GGTGACGTGG 
CTGATACTCA CAGCAGGTAC CATGGAAGAA AAACGGGAAG TCTTCTCATA TTTAGTACAC 
ATTGCAAAAT GCTGCTGGAA TATGGGCAAC TACAATGCTG TTATGGAATT CTTGGCTGGC 
CTCAGGTCAA GGAAAGTTTT AAAGATGTGG CAGTTTATGG ACCAGTCTGA TATTGAAACC 
ATGAGAAGCC TGAAGGATGC AATGGCCCAA CATGAATCTT CCTCTGAATA CAAGAAAGTG 
GTAAACCGGG CTCTGAATAT CCCTGGCTGT AAAGTGGTTC CTTTCTGTGG TGTCTTTTTA 
AAGGAGCTCT GTGAAATTCT AGATGGAACA TCTGGATTTA TGAAACTTTG CCCACGATAT 
AATTCACAAG ATGAAACTTT AGAGTTTGTA GCTGATTACA ATGGACAAGA TAATTTTTTA 
CAACGAGTAG GACAAGATGG CTTAAACAAC CCAGAGAAAG AATCCACTGT CAACAGTATT 
TTTCAAATTA TCCGAAGCTG CAATCGTAGT CTGGAACCTG AAGAAGAAGA CAGCTTAAGT 
GAAGGCGATG ATTCAAGGAA AAATTCCTTG AAAGATAAAA CCCGACTACA GTTTGTAATT 
GGAGAACTGT CTGATTCAGA AAGTGATATT TTTGAGCAGG CCAAAGAATG GGATTTCCAT 
GGAACGGAAG AAGAGCAGAA AGCATTCAAG CATGGCACAG AACTCATTCC CTGGTATGTC 
TTGTCAATTA GAGCTGATGT CCATCAGTTC ATACTCCAAG GAGCCACTGT TATTCACTAT 
GACCAGGATG CACATCTGTC AGCACGATGC TTTCTTCGAC TTCAACCTGA TAATAGTACT 
TTGACCTGGA TCAAACCTAC AACTGCCTCA CTCGCCAATA CTAAAGCCAA ATTAGGCATC 
ACAGGAAATT TAGCTGACCC TGGCAAACTT CAATCACTTG GCAATACTGG ATTAAATGGA 
CTGATGGAAG GTTTTCTGGA TTTGTTTTCA GTCAAGGCTG TGTACATGGG ACATCCTGGC 
ATTGATATGC ACACTGTGTG TGTTCAGAAC AAACTCAGTA ACATGTCCCT CTTAGAGAAT 
AGTGTGACAC TGCTTTATGG CCTTCAAACT ACTGAGAATA GATTATTACA CTTTGTGGCA 
CCAAAACATA CTGCTAAAAT GTTGTATAAT GGATTATTAG AATTAACAAA AGCTGTAAGA 
AAAATGAAGA AATTTCCTGA CCAGCGACAG CAGTGGCTAA GGAAGCAATA TGTCAGCCTC 
TATCAGGAGG ATGGAAGATT TGAAGGTCCA ACTTTGGCTC ATGCTATCGA ACTGTTTGGT 
GGCAGACGTT GGACTACACG AAATACAAGT CCAGCAACAC TTTCTAAAAG TGTGGAGAAA 
CCCATTGTGC AAAGGAACAA CACCCTTGGG ATAAGTGCAA ATAAGAAAAA GAAAAAAATC 
CTTATGAGGG GTGAAAGTGG AGAGGGTACT GATGATGAGA TGGCCACACG AAAGGCAAAA 
ATGCATAAGG AATGCCGTGG CCGCAGTGGC TCAGATCCTC AAGACCTTAA TGAGCAAGAA 
GAATCAGATC AGAATGTTAT TATTAGTCCC TGTAATAATC TGCCATCCAG GAGAGCCAAT 
TCTTTGACTA CAGCTGGATC TCCTAACTTG GCTGCTGGAG CAACTTCACC AGTCAGACCA 
CTTTCCTCTC CTGTATTGTC ACCTTCAAAT AAGAATCAGC CTAATACATG GAGCAGCAGT 
AGTTGGCATG GCCGAGTTAA AGGTGGACTG AAGGGATTTC AAAATTTCAT GGTTTCAGAT 
AGTAACATGA GTTTTGTTGA ATTTGTAGAG CTGTTCAAGT CATTCAGTGT ACGAAGTCGA 
AAGGACCTCA AGGATCTTTT TGACATCTAT GCTGTACCCT GCAGTCGATC AGGATCTGAG 
TCTGTCCCAC TCTATACAAA CCTGAGAATT GATGAAAATA CCAGTGGCCT TCAGCCTGAT 
TTAGATTTGT TAACCAGGAA CGTGTCTGAC TTGGGCTTGT TCATTAAGAA TAGGCAACAG 
TTATCAGACA ACCAGAGGCA GATATCTGAT GCCATCGCAG CTGCTAGTAT TGTGACTAAT 
GGTTCTGGAA TAGAAAGCAC CACGCTTGGT GTCTGTGGGG TTGGCATATT ACAGCTGAAT 
GACTTTTTAG TGAATTGCCA AGGGGAACAC TACACTTATG ATGAGATCCT CAGCATTATC 
CAGAAATTCG AGCCTAGTGT GAGTATGTAT CAGCAAGGTC TAATGTCATT TGAAGGATTT 
TCCAGGTTTC TGATGGATAA AGATAATTTT GCTTCCCAAA ATGATGAATC ACAGGAGAAT 
GTTAAAGAAT TGCAGTTACC ACTCTCCTAT TATTACATTG AATCTTCACA CAACACATAC 
CTCACAGGAC ATCAGCTTAA AGGAGAGTCC TCAGTGGAGC TATATAGCCA GGTCCTCTTG 
CAAGGCTGTC GGAGTGTGGA ATTAGACTGT TGGGATGGTG ATGATGGAAT GCCAATTATC 
TATCATGGAC ATACACTTAC TACCAAGATT CCCTTCAAGG AAGTAGTTGA AGCCATTGAT 
CGCAGTGCCT TCATCAACTC TGACTTGCCT ATTATAATAT CTATAGAAAA TCACTGTTCA 
TTGCCTCAGC AACGAAAAAT GGCAGAAATT TTCAAGAATG TATTTGGAGA AAAACTAGTT 
GCCAAATTTT TGTTTGAGAG TGATTTCTCT GATGATCCAA TGCTTCCTTC ACCTGACCAA 
CTTAGGAGAA AAGTACTTCT TAAAAACAAA AAACTGAAGG CACATCAGAC TCCAGTGGAC 
ATATTAAAGC AAAAGGCTCA TCAACTGGCA TCCATGCAAA CACAAAGCAA TAATGGTGGT 
AATGCCAATC CTATGTCTGC CAACAATGAA GAAGAAGAGG AGGAGGAGGA TGAATATGAT 
TATGACTATG AATCTCTTTC TGATGACAAC ATCCTGGAAG ACCGGCCTGA AAGTAAGTCA 
GCAAGTGACA AACTACAGTT TGAGTACAAT GAAGAAATTC CCAAGAGAAT CAAGAAGGCT 
GACAGCTCTG TTTGCAACAA GGGAAAGGTT TATGACATGG AACTGGGTGA AGAATTTTAT 
CTTCCACAGA ATAAAAAAGA GAGTAGACAG ATAGCTCCAG AGCTTTCTGA CCTTGTCATC 
TACTGCCAAG CTGTAAAATT TCCAGGATTG TCAACTTTAA ACCCATCTGG CTCTAACAGA 
GGAAAAGAAA GGAAAAGCAG GAAGTCCATT TTTGGCAACA ATCCGGGCAG ATTAAGCCCT 
GGGGAGACAG CAGCATATAC CAAAACATCT GGAAAAAGTC CCTTGGACGG CGTGCGGCAG 
ACCTGGGAAG AAGTCTGTTC TCTCAGCACA GCTCCGTCCC TCAGTGCCAT CATCAGGACC 
CCCAGGTGCT ACCACATCTC CTCCCTGAAC GAGAATGCGG CCAAGCGGCT GTGCCGCCGG 
TACTCTCGGA AGCTGATTCA GCACACCGCC TGCCAGCTGC TGAGAACCTA CCCTGCCGCC 
ACTCGCATTG ACTCCTCCAA CCCCAATCCC CTCCTGTTCT GGCTGCACGG GATACAGCTG 
GTGGCACTCA ACTACCAAAC AGACGATCTC CCCTTACACC TAAACACAGC AATGTTTGAA 
GCTAATGGTG GCTGTGGCTA TGTTTTGAAA CCCCCTGTTT TATGGGACAA GAATTGTGCT 
ATGTACCAGC AGTTTTCTCC ACTGGAAAGA GATATGGAAA ACATGGAACC TGCTCTCTAT 
TCTTTAACTA TTGTCTCTGG TCAGAATGTG TGTCCTAGTA ACAGTACAGG AAGTCCATGT 
ATTGAAATCG ATGTCCTGGG AATGCCTCTG GATAGCTGCC ATTTCCGCAC TAAACCCATT 
CATCGAAATA CTCTTAACCC CATGTGGAAC GAACAGTTTC TCTTCCGAGT TCATTTTGAA 
GATCTTGTAT TTCTTCGTTT TGCGGTTGTT GAAAACAACA GTTCAGCTGT AACTGCCCAA 
AGAATCATTC CACTGAAGGC TTTAAAAAGA GGATATCGGC ATCTTCAGCT GAGAAACCTC 
CATAATGAAG TCTTAGAAAT CTCTAGCTTG TTCATAAACA GCAGGAGGAT GGAAGAGCAT 
TCCAGTGGCA GTGCTGTGGC AGCATCTTTG ATGTTCAGCA CAGAAGAAAG AAAAGGTCTG 
ATGACATACA AGGTCACAGT ACATGGGATT CCAGGTCCTG AGCCTTTTGC CATTTTCATT 
ATAAATAAAG AAATTAAAGC AAAGCAGCTT CTTCACCAAA TTTTGACTGC TTCACAAAAC 
TCCATACCAG TCACTCCGGA CTGTTTTTTA ATGGAAGAAA AATATTTTAT ATCTAAAGAA 
AAAAATGACT TCAGGAAACA ACCATTCCAG AGAATAATCG GTCCAGAAGA AGAGATTGTG 
CAGATTTTGA ACAGCTGGTT CCCAGAAGAA GGTTATGTTG GAAGGATTAT TTTTAAAATC 
CAGGAGGAAC GCCCAGAAGA GAAGAACATC TTCCAAGAAG AGAAGGAGGT GATGCTGAGC 
TCAGAAGATG AAAGCTTCTT TGTACAAGTC CATGATGTTT CTCCCGAGCA GCCCCGAACA 
GTCATCAAAG CTCCCCGAGT CAGCACTGCC CAGGAGGTCA TCCAGCAGAC TCTATGCAAA 
GCAAAATATT CTTACAGCAT TTTGAGCAAT CCCAACCCGA GTGACTACGT GCTCCTAGAG 
GAGGTACTAA AGGAACCTGC TAGCAAGAAG TCTTCAACAC CCAAGTCTTC TCAGAGAGTT 
CTCGGGGACC AGGAGTGTGT GTTCCAGGCT CAAAGCAGGT GGAAGGGAGC CGGGAAATTT 
ATCCTTAAGC TAAAAGAGCA GGTTCAGGCA GCTCGAGATG ATAAAAAGAA AGGCATTTCC 
TTTGCTAATG AACTTAAGAA ATTTGCCAAG TCTAGCAAGC AGTATCGAGG ATTCACCTCA 
CCTTCTCAGC TTTCAATAGC AGAAAGTGTC CAGATGAAGG AAGAAAAATC TGTGGGCAGC 
TTCTCTTCTG AGACGACAGA CCATCACCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100917022 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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