HERC2 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	HERC2
Entrez Gene ID	100915156
Full Name	HECT and RLD domain containing E3 ubiquitin protein ligase 2
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031959088.1	XP_031814948.1	E3 ubiquitin-protein ligase HERC2 isoform X1
XM_023500395.1	XP_023356163.1	E3 ubiquitin-protein ligase HERC2
XM_012546136.1	XP_012401590.1	E3 ubiquitin-protein ligase HERC2
XM_031959089.1	XP_031814949.1	E3 ubiquitin-protein ligase HERC2 isoform X2
XM_031959091.1	XP_031814951.1	E3 ubiquitin-protein ligase HERC2 isoform X4
XM_031959090.1	XP_031814950.1	E3 ubiquitin-protein ligase HERC2 isoform X3

Pathways from BioSystems
Gene Ontology

The following HERC2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HERC2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa127559	XM_012546136.1 Latest version!	Sarcophilus harrisii E3 ubiquitin-protein ligase HERC2 (LOC100915156), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa152727	XM_031959088.1 Latest version!	Sarcophilus harrisii HECT and RLD domain containing E3 ubiquitin protein ligase 2 (HERC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa152727	XM_023500395.1 Latest version!	Sarcophilus harrisii HECT and RLD domain containing E3 ubiquitin protein ligase 2 (HERC2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa229206	XM_031959089.1 Latest version!	Sarcophilus harrisii HECT and RLD domain containing E3 ubiquitin protein ligase 2 (HERC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa229207	XM_031959090.1 Latest version!	Sarcophilus harrisii HECT and RLD domain containing E3 ubiquitin protein ligase 2 (HERC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSa229208	XM_031959091.1 Latest version!	Sarcophilus harrisii HECT and RLD domain containing E3 ubiquitin protein ligase 2 (HERC2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HERC2 ( XM_012546136.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012401590.1 Sarcophilus harrisii E3 ubiquitin-protein ligase HERC2 (LOC100915156), mRNA.

Price & Availability↑

CloneID	OSa127559
Clone ID Related Accession (Same CDS sequence)	XM_012546136.1
Accession Version	XM_012546136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	E3 ubiquitin-protein ligase HERC2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003831837.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTCTTCTG AATCCTTCTG TTTAGCTGCC CAGGCTAGAT TTGACTCCAA GTGGTTAAAA 
ACAGATTTAC AGCTTGCTTT TACAAGAGAT GGACTCTGTG GCCTATGGAA TGAAATGGTC 
AAAGATGGAG AAATTGTATA TCCTGGGACA GATGTAATAC AGAATGGAGA ACTACCTCCT 
CGAAAAGATG ACACCATGGA ATGTCAGAGT GGAACAAAGA AAGAAGATCA AAATGATAAA 
GAGAAGAAAG ATGAAGAGGA AATTCCAGTC CCTATGTATA AGGCCAAATC ACTTGTGGAG 
AGTTGGGTAT GGGGCAAACA ACCAGATGTG AACGAGTTGA AAGAGTGCCT TTATGTGCTA 
GTGAAAGAAC AGCAAACTCT TGCTACACGG ATGGCTACAA CAACTCTTTC AGCAGTAAGG 
CTAAAGCAGA GGCTGGTTAT ACTGGAACGA TATTTCATTG CACTGAATAG AACTGTTCTT 
CAGGAAAATG TCAAAGTTAA GTGGAAAAGC AACAGTATTC CTTTACCTAC TGTCGACAAA 
AAAAGTGATG TTCATGGAAC TGCAAGTACC AAAGGACCAG GAAACATTCC ATTACAAGAC 
CAGCACTTAG CACTTGCCAT ATTGTTAGAG TTGGCTGTGC AGAGAGGCAC TCTAAGCCAA 
ATGTTATCTG CCATTATGTT ACTACTTCAA TTATGGGACA ATGGGACAAG GGAAACAGAT 
AATGAACGAT CTGCCCAAGG AACTAGTGCA CCACTTCTCC CTTTATTACA AAGATTTCAG 
AGTATAATCT GCAATAAAGA CATACTTAAT ACTGAAGATG AGATTCAGAT GCTGTCTGGT 
CTTCTGAGTC CCAATGAAAG TTTTTTGAGG TACCTTACTT TACCACAAGA CAATGAATTA 
GCCATTGATT TGCGTCAAAC TGCAGTAGTG ATTATGGCGC ATTTAGATCG TCTGGCCACT 
CCCTGTATGC CTCCTCAGTG TAGTTCTCCA ACATCTCATA AGGGATCTTT ACAAGAAGTC 
ATAGGTTGGG GCTTAATAGG ATGGAAATAC TATGCTAATG TGATTGGCCC AATTCAATGT 
GAAGGACTTG CAAATCTTGG GATCACACAG ATTGCCTGTG CAGAAAAGCG TTTCCTGATT 
TTGTCTCGGA ATGGGCGAGT TTACATGCAG GCTTACAACA GTGACACATT GGTAGGGGTG 
GTTGCTGTTT TTTCCCTAAT TTTTGGTCAA GTCTGGTCTT GGGGCGATGG TGATTATGGA 
AAACTAGGAA GAGGTGGTAG TGATGGTTGT AAAACACCAA AACTGATTGA AAAACTTCAA 
GACTTAGATG TTGTTAAAGT ACGTTGTGGA AGCCAATTTT CCGTTGCTTT AACAAAAGAT 
GGTCAAGTTT ACTCGTGGGG AAAAGGTGAC AATCAGAGAC TTGGGCATGG AACAGAGGAG 
CATGTTCGAT ATCCGAAACT GTTAGAAGGC TTACAAGATA CAAAACATAT AGTTGGAATT 
GCTTGTGGGC CCGCTCAGAG TTTTGCTTGG TCTTCTTGTT CTGAGTGGTC CATTGGCCTA 
CGTGTACCTT TTGTGGTGGA TGTTTGTCCC ATGACATTTG AACAGTTGGA TCTCTTACTA 
CGACAAGTAA GTGAGGGAAT GGATGGCACC TCTGACTGGC CACCACCACA GGAGAAAGAA 
TGTATGGCAG TGGCAACATT GAACCTTCTG AGACTTCAGT TGCATGCTGC TATTAGTCAC 
CAGGTTGATC CGGAATGTCT TGGATTGGGT CTGGGAAGTG TACTCTTGAA TAGCTTGAAG 
CAGACAGTTG TAACACTAGC AAGTAATGCT GGAGTTCTTA ATACAGTGCA ATCAGCTGCT 
CAAGCAGTAC TGCAGAGTGG ATGGTCTGTG CTGCTTCCAA CTGCAGAGGA ACGAGCAAGG 
GCTCTTTCAG CTCTCTTACC CAGTGCAGTT TCAGGAAATG ACATGAATGT AAGTCCAGGA 
CGTCGTTTTA TGATTGATCT TCTTGTAGGC AGTTTGATGG CTGATGGAGG ATTAGAATCA 
GCATTAAATG CTGCCATCAC TGCAGAAATC CAGGATATTG AAGCAAAAAA AGAAGCTCAA 
AAGGAAAAAG AGATTGATGA GCAGGAAGCA AATGCTTCAA CACTTCATCG GAGTAGGACA 
CCATTAGATA AAGACCTCAT TAATACTGGA ATTTATGAAT CTGCCGGAAA ACAGAGTTTG 
CCTCTGGTTC AATTAATACA ACAGCTTCTT AGAAATATTG CTTCCCAGAC AATAGCCAAA 
TTGAAAGATG TTGCTCGTCG AATTTTGTCC TGTATAGAAC ATGATCAATA TAGTCAAGAC 
AGATCTGCCT CCCTGGATTT GTTGCTTCGT TTTCAGCGTC TGCTGGTCAG TAAACTCTAT 
CCAGGAGACA GTGGTGGTCA AGTTTCAAAT TCCTTTAGTC CTGAGCTATT AGGAGTTGGT 
TCTTTGCTGA AAAAATATAC AGCTCTTTTG TGCACACACA TTGGAGATAT ATTGCCTGTG 
GCAACTAGTA TTGCATCTTC CAGTTATCGG CATTTTGCAG AAGTATCTCG TGTTGTGGAT 
GGTGATCTAA CAGGTGTTCT TCTTCCAGAG TTGATAGTAT CTATTGTCCT TTTGCTCAAT 
AAAAGTCCTG GATTAATGCA GGAAGCTGGG GCAATTCCTC TCTTAGCTGG TCTACTGGAA 
CATCTAGATA GATTTAACCA TTTGGCACCA GGAAAAGAGA GAGATGATAA TGAGGATTTA 
GCCTGGCCTG GAATAATGGG ATCATTTTTT ACAGGTCAGA ATAGTAGAAA TAATGAGGAA 
GTGACGCTCA TACGTAAAGC TGATCTGGAG AATCACAATA AAGATGGAGG CTTCTGGACT 
GTGATTGACG GGAAAGTGTA TGATATAAAA GACTTTCAGA CACAGTCTTT AACAGGCAAT 
AGCATTCTTG CTCAGTTTGC AGGTGATGAC CCGGTGGTTG CTTTGGAAGC TGCTTTACAG 
TTTGAGGACA CAAGGGATTC AATGCATGCA TTTTGTGTTG GCCAGTATAT GGAGCCTGAC 
CAGGAAGTGA TTACAACGCC AGACCTTACC AGTCTCTCTT CACCTCTCAT AGATCCAGAA 
AGAAATCTGG GTCTCCTCCT TGGACTTCAT GCTTCCTATT TAGCAATGAG CACACCACTA 
TCTCCTGTGG AGATTGAATG TGCCAAATGG CTTAAGTCAT CCATATTCTC TGGAGGATTG 
CAAACCAGTC AGATACATTA TAGTTACAAT GAAGAGAAAG ATGAAGACCA CTGCAGTTCT 
CCTGGAAATA CTACTCCAAA CAAATCCAAA CTCTATTCCC ATAGATTGAC TTTGAGTGAT 
CATTCCCAAC CATTTTTGCA AGCCATTGCA GATAATAACA TTCAGGATCA TACCGTGAAG 
GATTTTTTAT GTCAGATAGA AAGGTATTGC AAGCAATGCC ATTTGACCAC ACCGATAACA 
TTTCCTCCTG AACATCCTGT GGAAGAAGTG GGACGTTTAC TCTTATGTTG TCTTCTAAAG 
CATGAAGATT TAGGTCATGT GGCCTTATCT TTAGTTCACC CAGTTACACT TGGTGTTGAC 
CAAGTCAAAC ACAGAACGTT GCCTAAGTCT GTAGTGGATG TTTGTAGAGT CGTCTACCAG 
GCAAAATGCT CACTAATAAA GACACATCAG GAACAGGGAC GTTCTTATAA GGAAGTGTGT 
GCTCCTGTTA TTGAACGTTT GCGATTTCTT TTTAATGAAT TGCGTCCTGC AGTCTGCAAT 
GACCTCTCAA TAATGTCCAA ACTTAAACTT TTAAGCTCTT TGCCTCGTTG GAGAAGAATA 
GCTCAGAAGA TGATACGAGA GAGAAGGAAA TTAAAAAGAG TACCCAAAAA GTCAGAACCT 
ACTGATGTGG AAGAATCTAA GATTGGCAAT GAAGAGGGTG ACTTAGAAGA AGCCTGTATT 
CTTCCTCATA GTCCTGTATC TGTAGATAAA AGACCTGTTC CAGTTAAATC ACCTAAGGAT 
AAATGGCAGC CACTTCTGAG TACAGTTACA GGTGTTCACA AATACAAATG GCTTAAACAG 
AATGTTCAAG GCCTTTATCC TCAGTCTGCA CTGCTTAGCA CAATTGTTGA ATTTGCGCTT 
AAAGAAGAGC CAGTTGATGT GGAAAAGATG AGAAAGTGCT TACTCAAACA GTTGGAGAGA 
GCAGAAGTTC GTTTGGAAGG AATAGACACG ATTTTAAAAT TGGCAAATAA AAATTTCTTG 
CTTTCGTCTG TGCAATATGC TATGTTCTGT GGATGGCAAA GACTTATCCC AGAAGGAACC 
AATATAGGGG AACCACTTAC AGATTGTTTA AAGGATGTTG ATCTGATTCC ACCATTTAAT 
CGAATGCTAC TAGAAGTAAC TTTTGGAAAG TTGTATGGTT GGGCCATTCA GAATATTCGA 
AATGTTTTGT TGGATGCAAA TGCCAGATTT AAAGAGCTAG GTATCCAGCC TGTTCCTCTG 
CAAACTATTA CTAATGAGAA TCCAGCAGGG CCAAGCCTTG GAACCATCCC ACAAGCACGT 
TTTCTCCTGG TTATGCTCAA CATGCTTACC CTTCAATATG GTGCAAACAA CTTGAATCTT 
CTTTTGAACT CTGGCATGCT AGCATTGACT CAGACAACAT TGCGATTAAT AGGTCCTAGT 
TCTGATAATA TCGAGGAAGA AATGAATGCC TCTGCACGAG GAGCTTCAGC TACTGTTTTG 
GAAGAATCCA GAAAAGAAAC CACTCCAGTA CAACTTCCTG TTTCAGGGCC AGAACTGGCA 
GCAATGATGA AAATTGGTAC CAGGGTCATG AGGGGAGTGG ACTGGAAATG GGGAGATCAG 
GATGGTCCAC CTCCAGGTTT AGGTCGTGTG ATTGGTGAAT TGGGAGAGGA TGGATGGATA 
AGGGTTCAGT GGGATACAGG CAGTACTAAT TCATACCGAA TGGGAAAGGA AGGAAAGTAT 
GATCTTAAAT TGGCTGAGCC TCCACCTGCA GCTCAGCCCT CAACAGAGGA TTCGGACACT 
GAGGATGATT CTGAAAGTGA ACAAATGGAA AGAAACTTCC ACCCTACTTC TATTATGCTT 
ACAAGTACTG TTAATTTACT CCAAACTCTC TGTCTCTGTG CTGGAATTCA TGCAGAAATC 
ATGCAAAATG AAGCTACAAG AACTCTGTGT GGTCTACTTA GAATGTTAGT GGAAAGTGGA 
ACAGTAGATA AAACATCTTC TTTACCAAAT AAGATGGTTT ATAAAGAGCA GCACAGAAGT 
TGGTGTACAC TTGGATTTAT TCGAAGTATA GCACTTATGC CTCAAATGTG TAGCACCCTT 
AGCACATCTC AGTGGATCAC ATTACTAATG AAAATTGTGG AAGGACACGA GTCATTTACA 
GCAGCTTCGC TTCAGAGACA GATCTTAGCA GTACATTTAC TACAAGCTGT ACTTCCATCT 
TGGGACAAAA CAGAAAGATC AAGAGATATG AAGTTTCTTG TGGAAAAACT TTTTGGCTTC 
TTGGGCAGTC TACTTAGTAC ATGCTCTTCA GACATACCTT TGCTCAGAGA ATCAACTCTG 
AGAAGGAGGA AGTCCAGACC CCAGGCATCT TTGACTGCAA CCCATAGTAG TACTTTGGCA 
GAGGAGATAG TGGCCCTGCT AAGGACTCTA CATTCACTGA GCCAGTGGAA TGGACTCATA 
AACAAGTACA TCAACTCTCA GCTAAACTCC ATCACCCACA TCTTTGCAGG AAAACAGTCT 
GAAGGGACTG TGTTAGAAGA TTACTTTCCT GATTCTGAGA ACCCTGAAGT TGATGGCCTA 
ATGGCAGTGC TAGCTGTAAT TGGTGGAATA GATAGTCGAT TGAGGCTGGG TGGCCAAGTG 
GTACATGATG AATTTGGAGA AGGCACAGTG ACACGCATCA CTCCTAAAGG CAAAATCACA 
GTCCAGTTCT GTGATATGCG AATATGTAGA ATTTGTCCAT TGAGTCAGCT AAAACCGCTT 
CCAGTTATGG CATTTAATGT GAACAACTTA CCATTCACGG AATCTATGCT CTCTGTCTGG 
GCTCAATTGG TCAACTTGGC AGGGAGCAAA TTAGAAAAAC ACAGAATTAA GAAATCTACT 
AATCAAGCCC TCACAGGACA AGTAGACTTG GATTTGCTAA GATGCCAACA GTTGAAATTA 
TACATATTGA AAGCAGGTCG AGCATTGCTT TCCCATCAGG ATAAACTGAG GCAGATCTTA 
TCTCAACCAG CTTTACAAGA GAATGGAGTT CTCTCTACAG ATGATGGTAC AGCATCCCCT 
GATATTGGAG ATATGTCTCC TGAAGGACCT CAACCTCCAA TGATCCTTTT ACAGCAATTG 
TTGACATCTG CCACACAGCC ATCACCTGTA AAAGCAATAT TTGACAAACA GGAACTTGAG 
GCTGCTGCTT TGGCTGTATG CCAGTGTCTA GCTGTGGAGT CAACTCATCC ATCAACTCCA 
GTGTTTGAAG AATGTAGCTC TAGTGAGGCC ACAACACCTG TCACAGTCCA GCATATTCGA 
TCTGTAAAAG TGAAAAAGCG AAAGCAGTCT CCTCTCCCTG CTTTGCCTAT TGTAGTACAG 
CTCATGGAAA TGGGATTTCC GAGGAAAAAC ATTGAATTTG CATTGAAGTC TCTTACTGGG 
AGTGCTTCAG GGTTACCAGG TGTTGAAGCC TTGGTTGGTT GGTTGCTGGA TCATCCTGAT 
GTCCAAATTA CTGATCTCTC AGATGCTGAT ACAGTCTCTG ATGAATACTC TGATGATGAA 
ATAGTGGAAG ATGTGGAAGA GACTGAAGCA TCTTACCCTG TGTCTAGTTG TGCTGTTGTA 
ACGGAGAGTC ACACTTATAA AAAACGAGCT GATTTCTTAA GTAATGATGA CTATGCAGTA 
TATGTTAGAG AAAACATTCA GGTAGGAATG ATGGTTAGGT GCTGCAGAAC GTATGAAGAA 
GTTTGTGAAG GAGATGTAGG TAAAGTGATT AAATTGGACA GAGATGGATT ACACGACCTC 
AATGTGCAGT GTGATTGGCA ACAAAAGGGG AGTACCTATT GGGTTAGGTA TATCCATGTT 
GAACTTATAG GTTTCCCACC ACAGAGTTCT CCTTCCCATA TCAAGATTGG TGATAAAGTT 
AGGGTGAAAA CATCAGTAAC CACACCAAAA TATAAATGGG GATCAGTTAC TCATAGAAGT 
GTGGGAGTTG TGAAAGCTTT TAGTGCCAAT GGAAAAGATG TTATTGTGGA TTTTCCTCAG 
CAGTCTCATT GGACAGGATT ATTGTCAGAA ATGGAATTGG TGCCTAGTAT TCATCCTGGA 
GTTACGTGTG ATGGCTGCCA AATGTTTCCT ATCAATGGTC CTCGTTTCAA ATGCAGGAGT 
TGTGATGATT TTGACTTTTG TGAGACCTGT TTTAAAACCA AGAAGCATAA TACCAGACAT 
AGTTTTGGCA GAATAAATGA ACCAGGTCAG TCTCTTGTAT TTTGTGGGCG TTCTGGGAAA 
CAGCTAAAAC GTTGCCACAG TAGTCAGCCT GGAATGCTCT TGGATAATTG GTCAAGAATT 
GTTAAGAATT TAAATGTGTC CTCATCAGTA AATCAGGCTT CTCGCTTGAT TGATGGCAGT 
GAACCATGTT GGCAGTCATC TGGATCACAA GGAAAGCATT GGATTCGACT GGAGATTTTC 
CCCGATGTTC TTGTTCATAG ACTGAAAATG ATAGTAGATC CTGCTGACAG TAGCTACATG 
CCTTCACTAG TTGTAGTTTC AGGTGGGAAT TCCCTAAATA ATTTAATTGA ACTGAAGACA 
ATCAATATTA ACCCAGCTGA TACCACAGTG CCTCTTCTAA ATGACTGCAC AGAGTATCAC 
AGGTATATTG AGGTTGCCAT AAAGCAATGC AGAAGCTCAG GAATAGATTG TAAAATTCAT 
GGTCTTATTA TACTGGGACG AATTCGGGCA GAAGATGAAG ATTTGGCTGC TGTTCCTTTC 
TTAGCGTCAG ATAATGAAGA AGAAGATGAT GAAAAAGCTA ACACTGGGAG TCTTATTAGG 
AAGAAGGCTT CTGGATTGGA ATCAGCAGCC ACAATAAGGA CCAAAGTTTT TGTCTGGGGG 
CTGAATGACA AGGATCAATT AGGAGGATTA AAGGGTTCTA AGATAAAAGT TCCTTCATTC 
TCTGAAACCC TTTCTGCTTT GAATGTAGTT CAAGTGGCAG GTGGATCTAA AAGTTTGTTT 
GCAGTGACTG TGGAGGGTAA AGTGTATGCT TGTGGAGAAG CTACTAATGG ACGACTGGGT 
TTAGGTATTT CTAGTGGAAC TGTACCCATT CCGCGGCAGA TTACAGCTCT CAGCAATTAT 
GTGGTAAAGA AAGTTGCTGT TCACTCAGGT GGCCGGCATG CTATGGCTTT AACTGTTGAT 
GGAAAGATAT TTTCATGGGG TGAAGGTGAT GATGGAAAAC TAGGACACTT CAGCAGAATG 
AACTGTGATA AACCAAGACT GATAGAGGCA TTGAAAACCA AACGTATTCG TGATATAGCT 
TGTGGTAGTT CTCACAGTGC TGCCATTACA TCAAGTGGTG AATTGTATAC ATGGGGCCTT 
GGAGAATATG GCCGGTTGGG ACATGGGGAT AATACTACAC AGCTGAAACC CAAAATGGTG 
AAAGTTCTTC TTGGTCATCG AGTTATTCAA GTTGCTTGTG GAAGTAGAGA TGCCCAGACT 
CTGGCTTTGA CAGATGAAGG TTTGGTCTTT TCCTGGGGAG ATGGTGACTT TGGGAAACTG 
GGCCGGGGAG GAAGTGAAGG ATGTAACATT CCTCAGAACA TTGAGAGACT TAATGGACAA 
GGAGTTTGCC AGATTGAGTG TGGGGCTCAA TTTTCCTTGG CACTTACAAA GTCAGGAGTA 
GTATGGACTT GGGGTAAAGG AGATTACTTT AGGCTTGGTC ATGGCACTGA TGTTCATGTG 
AGAAAACCAC AAGTAGTAGA AGGACTACGA GGAAAGAAGA TAGTTCATGT GGCAGTAGGC 
GCTCTTCACT GCTTAGCTGT CACTGATACA GGTCAAGTGT ATGCTTGGGG TGACAATGAT 
CATGGACAAC AAGGCAATGG GACTACTACT GTCAACAGAA AGCCTACCCT TGTACAAGGC 
TTAGAAGGAC AAAAAATCAC TCGGGTAGCT TGTGGCTCAT CTCATAGTGT GGCGTGGACA 
ACAGTGGATG TAGCAACCCC ATCTGTTCAT GAACCTGTCC TCTTTCAAAC TGCTAGGGAC 
CCTTTAGGCG CTTCTTACTT AGGTGTTCCT TCCGATGCTG ATTCTTCTGC TGCCAGTAAC 
AAAATCAGTG GTGTAAATAG TTCCAAGCCT AACCGCCCTT CCCTTGCTAA GATTCTCTTG 
TCTTTGGATG GAAACCTTGC CAAACAGCAA GCATTATCTC ATATACTTAC AGCATTGCAA 
ATTATGTATG CGAGAGATGC AGTAGTTGGA GCATTAATGC CTGCTAGTAT GATCGCACCA 
GTGGAATGCT CATCTTCTTC ATCACCTGCA CCCCCCTCAG ATGTGTCTTC AGCAGGAAGT 
CCCATGAATG GTGAAGAGTC TGTGTTAGCT GTGGATGTAG AAGATAGACT AAGCCCAAAT 
CCATGGCAAG ACAAGAGAGG GGAGGTAGTT TCTTCAGAAG ATGCTGTGAC TCCATCAGCA 
GTGACTCCCT CAGCTTCCTC TGCCTCTTCT CGACCATTTA TTCCAGTAAC TGATGATCCT 
GGAGCTGCCA GTATCATTGC AGAAACCATG ACTAAAACCA AAGAAGATGT TGAAAGCCAA 
AATAAAGTAT CAGGTCCGGA ACCTCAATAT TTGGATGAAT TTACTAGTCT CTTAGTGCCA 
GATGATACCC GTGTCATGGT TGACTTGCTC AAGCTGTCTG TTTCTAATCG AGCTGGTGAA 
AAGGGAAAAG ATGTTCTTTC AGCTGTGCTT TCTGGTATGG GCACTGCTTA TCCACAGGTA 
GCTGATATGT TGTTGGAGCT CTGTGTTACA GAGTTAGAAG ATGTTGCCAC AGACTCTCAG 
AGTGGTCGAC TCTCTTCCCA ACCTGTTGTA GTAGAAAGTA GTCATCCGTA TACTGATGAT 
ACCTCTACCA GTGGTACTGT CAAAATACCA GGTGCTGAAG GACTCAGAGT AGAGTTTGAT 
CGACAATGTT CTACAGAACG GCGTCATGAC CCTCTCACAA TCATGGATGG TGTCAATAGG 
ATAGTTTCAG TTCGATCAGG TCGAGAATGG TCTGATTGGT CCAGTGAACT TCGAATTCCT 
GGAGATGAAT TAAAGTGGAA GTTCATCAGT GATGGATCAG TGAATGGATG GGGTTGGCGA 
TTCACTGTCT ATCCTATTAT GCCAGCTGCA GGCCCTAAAG ATCTTCTTTC AGATCGGTGT 
ATTCTTTCCT GTCCCTCAAT GGACTTGGTT ACATGCTTGT TGGACTTTAG ATTAAATTTA 
GCTTCCAATA GAAGTATTGT TCCTCGTTTG GCAGCATCAC TTGCAGCATG TGCTCAATTG 
AGTGCTCTAG CTGCTGGTCA CAGAATGTGG GCCCTTCAAA GACTGAGGAA ACTGCTTACA 
ACTGAATTTG GACAATCAAT CAATATAAAT AGGATACTTG GAGATAATGA TGGAGAAACA 
AGAACCATGA GTTTTACCGG TAGTGCTTTA GCAGCTTTGG TAAAAGGTCT TCCAGAAGCT 
TTGCAAAGAC AGTTTGAATA TGAAGATCCG ATTGTTAGAG GAGGCAAACA ACTGCTCCAT 
AGCCCTTTCT TTAAGGTGCT GGTAGCTCTT GCTTGTGACC TAGAACTGGA TACTCTTCCT 
TGCTGTGCTG AGACACACAA ATGGGCTTGG TTTAGAAGAT ACTGTATGGC CTCTAGAGTT 
TCAGTAGCCT TGGACAAACG AACACCATTA CCTCGCCTTT TCCTTGATGA GGTGGCAAAG 
AAAATCAGGG AGTTAATGGC CGACAGTGAA AATCTGGATG TTCTGCATGA GAGCCATGAC 
ATTTTTAAGA GAGAACAAGA TGAACAACTC GTCCAGTGGA TGAACAGGAG ACCTGATGAC 
TGGACTCTTT CTGCTGGAGG CAGTGGAACA ATTTATGGTT GGGGTCACAA TCATCGAGGA 
CAGCTTGGCG GTATTGAGGG AGCAAAAGTC AAAGTTCCAA CACCTTGTGA AGCTCTTGCA 
GCTCTGCGGC CCATACAGTT AATTGGAGGG GAACAGACTC TCTTTGCGGT AACTGCTGAT 
GGGAAGCTTT ATGCTACAGG GTATGGTGCA GGTGGCAGAT TGGGAATTGG AGGAACTGAA 
TCAGTTTCTA CTCCAACATT GCTTGAATCT ATTCAGCATG TATTTATAAA AAAAGTTGCT 
GTGAACTCTG GAGGAAAACA CTGCTTAGCA CTCTCATCAG AAGGAGAAGT TTATTCTTGG 
GGTGAGGCAG AAGATGGAAA ATTGGGGCAT GGAAACAGAA GCCCCTGTGA TCGCCCACGT 
GTAATTGAAT CTTTGCGAGG AATAGAAGTT GTTGATATTG CTGCAGGTGG AGCCCACAGT 
GCATGTATTA CAGCAGCTGG TGACCTCTAT ACGTGGGGTA AAGGAAGATA TGGTCGCCTA 
GGTCATGGGG ATAGTGAAGA TCAGTTAAAA CCAAAACTGG TGGAAGCTCT CCAAGGCTAT 
CGAGTTATTG ATATTGCCTG TGGCAGTGGA GATGCACAGA CACTTTGCCT TACTGATGAT 
GATACTGTAT GGTCCTGGGG TGATGGGGAC TATGGAAAAC TTGGCAGAGG AGGCAGTGAT 
GGCTGTAAAG TGCCAATGAA GATTGATTCT CTCACAGGTC TTGGAGTGGT TAAAGTAGAG 
TGTGGATCAC AATTTTCTGT TGCACTTACC AAGTCTGGAG CAGTTTATAC TTGGGGTAAA 
GGCGATTATC ACAGGTTGGG TCATGGATCA GACGACCATG TTAGACGGCC TCGGCAAGTG 
CAAGGCCTAC AAGGAAAGAA AGTAATAGCA ATTGCTACTG GTTCTTTACA CTGTGTGTGC 
TGCACTGAAG ACGGTGAAGT TTATACTTGG GGTGACAATG ATGAAGGGCA ACTTGGGGAT 
GGAACAACTA ATGCAATCCA GAGACCTCGG TTGGTGGCAG CACTTCAGGG TAAAAAGATA 
AATAGGGTGG CTTGTGGTTC AGCACACACC TTAGCGTGGT CCACGAGTAA ACCTGCCAAT 
GCTGGGAAGC TTCCAACACA GGTTCCTATG GAATACAACC ATTTACAGGA ATTTCTCATA 
ATTCCTTTAC GAAACAGACT TTTGTTATTA CATCATATCT CAGAACTCTT TTGTCCATGC 
ATTCCCATGT TTGACCTTGA GGGGCGTCTT GATGAAACTG GCCAAGGACC TTCAGTGGGA 
TTTGACACAT TACGAGGAAT TCTGATATCT CAGGGAAAGG AAGCAGCTTT CCGAAAAGTT 
GTACAAGCAA CTATGGTGAG AGATCGTCAA CATGGTCCTG TAGTTGAATT AAACAGAATA 
CAGGTAAAAC GATCAAGAAG TAAGGGTGGT TTGGCTGGTC CTGATGGTAC TAAATCTGTT 
TTTGGCCAAA TGTGTGCTAA AATGATTTCA TTTAGTCCAG ACAGTCTTCT TCTACCTCAT 
CGAGTATGGA AAGTCAAGTT TGTTGGCGAG TCTGTGGATG ATTGTGGAGG TGGTTATAGT 
GAATCAATAG CAGAAATGTG TGAAGAACTT CAGAATGGAC TTACTCCTCT CCTAATTGTA 
ACTCCAAATG GCAGGGATGA ATCTGGAGCC AATAGAGATT GCTTCTTATT GAACCCAGCT 
GCCAGATCTT CTTTGCATAC CAATATGTTT CGTTTCTTGG GTGTATTGCT AGGCATTGCC 
ATTCGAACTG GGAGTCCATT AAGCCTCAAC CTTGCAGAAC CGGTTTGGAA GCAACTGGCA 
GGAATGAACC TTACAATTGC TGATCTCAGT GAGGTTGATA AGGATTTTAT TCCTGGCCTG 
ATGTACATTC GGGACAATGA AGCTACTTCT GAGGAATTTG AAGCAATGAG CCTTCCCTTC 
ACAGTGCCAA GTGCAAGTGG GCAGGATATT CAATTGAGCT CTAAATATAC CCACATCACA 
TTGGATAACC GTGCTGAGTA TGTTAGATTG GCAATAAATT ACAGGTTTGT TTTGGATAAG 
TACAATCCAC CTGACCACTT CCTTCCAGAG TCATATACAT GCTTCTTTTT GCTGAAGTTA 
CCCAGGTATT CCTGTAAACA AGTACTAGAG GAAAAACTGA AATATGCCAT TCATTTTTGC 
AAGTCTATAG ACACTGATGA TTATGCCCGG ATAGCACTCA CAGGAGAACC AGCTGCTGAT 
GACAGCAGTG ATGATTCAGA TAATGAAGAT GCAGATTCAT TTGCATCGGA CTCTACTCAA 
GATTATTTAA CAGGACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012401590.1
CDS	109..13509
Translation	MSSESFCLAAQARFDSKWLKTDLQLAFTRDGLCGLWNEMVKDGEIVYPGTDVIQNGELPPRKDDTMECQSGTKKEDQNDKEKKDEEEIPVPMYKAKSLVESWVWGKQPDVNELKECLYVLVKEQQTLATRMATTTLSAVRLKQRLVILERYFIALNRTVLQENVKVKWKSNSIPLPTVDKKSDVHGTASTKGPGNIPLQDQHLALAILLELAVQRGTLSQMLSAIMLLLQLWDNGTRETDNERSAQGTSAPLLPLLQRFQSIICNKDILNTEDEIQMLSGLLSPNESFLRYLTLPQDNELAIDLRQTAVVIMAHLDRLATPCMPPQCSSPTSHKGSLQEVIGWGLIGWKYYANVIGPIQCEGLANLGITQIACAEKRFLILSRNGRVYMQAYNSDTLVGVVAVFSLIFGQVWSWGDGDYGKLGRGGSDGCKTPKLIEKLQDLDVVKVRCGSQFSVALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQDTKHIVGIACGPAQSFAWSSCSEWSIGLRVPFVVDVCPMTFEQLDLLLRQVSEGMDGTSDWPPPQEKECMAVATLNLLRLQLHAAISHQVDPECLGLGLGSVLLNSLKQTVVTLASNAGVLNTVQSAAQAVLQSGWSVLLPTAEERARALSALLPSAVSGNDMNVSPGRRFMIDLLVGSLMADGGLESALNAAITAEIQDIEAKKEAQKEKEIDEQEANASTLHRSRTPLDKDLINTGIYESAGKQSLPLVQLIQQLLRNIASQTIAKLKDVARRILSCIEHDQYSQDRSASLDLLLRFQRLLVSKLYPGDSGGQVSNSFSPELLGVGSLLKKYTALLCTHIGDILPVATSIASSSYRHFAEVSRVVDGDLTGVLLPELIVSIVLLLNKSPGLMQEAGAIPLLAGLLEHLDRFNHLAPGKERDDNEDLAWPGIMGSFFTGQNSRNNEEVTLIRKADLENHNKDGGFWTVIDGKVYDIKDFQTQSLTGNSILAQFAGDDPVVALEAALQFEDTRDSMHAFCVGQYMEPDQEVITTPDLTSLSSPLIDPERNLGLLLGLHASYLAMSTPLSPVEIECAKWLKSSIFSGGLQTSQIHYSYNEEKDEDHCSSPGNTTPNKSKLYSHRLTLSDHSQPFLQAIADNNIQDHTVKDFLCQIERYCKQCHLTTPITFPPEHPVEEVGRLLLCCLLKHEDLGHVALSLVHPVTLGVDQVKHRTLPKSVVDVCRVVYQAKCSLIKTHQEQGRSYKEVCAPVIERLRFLFNELRPAVCNDLSIMSKLKLLSSLPRWRRIAQKMIRERRKLKRVPKKSEPTDVEESKIGNEEGDLEEACILPHSPVSVDKRPVPVKSPKDKWQPLLSTVTGVHKYKWLKQNVQGLYPQSALLSTIVEFALKEEPVDVEKMRKCLLKQLERAEVRLEGIDTILKLANKNFLLSSVQYAMFCGWQRLIPEGTNIGEPLTDCLKDVDLIPPFNRMLLEVTFGKLYGWAIQNIRNVLLDANARFKELGIQPVPLQTITNENPAGPSLGTIPQARFLLVMLNMLTLQYGANNLNLLLNSGMLALTQTTLRLIGPSSDNIEEEMNASARGASATVLEESRKETTPVQLPVSGPELAAMMKIGTRVMRGVDWKWGDQDGPPPGLGRVIGELGEDGWIRVQWDTGSTNSYRMGKEGKYDLKLAEPPPAAQPSTEDSDTEDDSESEQMERNFHPTSIMLTSTVNLLQTLCLCAGIHAEIMQNEATRTLCGLLRMLVESGTVDKTSSLPNKMVYKEQHRSWCTLGFIRSIALMPQMCSTLSTSQWITLLMKIVEGHESFTAASLQRQILAVHLLQAVLPSWDKTERSRDMKFLVEKLFGFLGSLLSTCSSDIPLLRESTLRRRKSRPQASLTATHSSTLAEEIVALLRTLHSLSQWNGLINKYINSQLNSITHIFAGKQSEGTVLEDYFPDSENPEVDGLMAVLAVIGGIDSRLRLGGQVVHDEFGEGTVTRITPKGKITVQFCDMRICRICPLSQLKPLPVMAFNVNNLPFTESMLSVWAQLVNLAGSKLEKHRIKKSTNQALTGQVDLDLLRCQQLKLYILKAGRALLSHQDKLRQILSQPALQENGVLSTDDGTASPDIGDMSPEGPQPPMILLQQLLTSATQPSPVKAIFDKQELEAAALAVCQCLAVESTHPSTPVFEECSSSEATTPVTVQHIRSVKVKKRKQSPLPALPIVVQLMEMGFPRKNIEFALKSLTGSASGLPGVEALVGWLLDHPDVQITDLSDADTVSDEYSDDEIVEDVEETEASYPVSSCAVVTESHTYKKRADFLSNDDYAVYVRENIQVGMMVRCCRTYEEVCEGDVGKVIKLDRDGLHDLNVQCDWQQKGSTYWVRYIHVELIGFPPQSSPSHIKIGDKVRVKTSVTTPKYKWGSVTHRSVGVVKAFSANGKDVIVDFPQQSHWTGLLSEMELVPSIHPGVTCDGCQMFPINGPRFKCRSCDDFDFCETCFKTKKHNTRHSFGRINEPGQSLVFCGRSGKQLKRCHSSQPGMLLDNWSRIVKNLNVSSSVNQASRLIDGSEPCWQSSGSQGKHWIRLEIFPDVLVHRLKMIVDPADSSYMPSLVVVSGGNSLNNLIELKTININPADTTVPLLNDCTEYHRYIEVAIKQCRSSGIDCKIHGLIILGRIRAEDEDLAAVPFLASDNEEEDDEKANTGSLIRKKASGLESAATIRTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALNVVQVAGGSKSLFAVTVEGKVYACGEATNGRLGLGISSGTVPIPRQITALSNYVVKKVAVHSGGRHAMALTVDGKIFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAAITSSGELYTWGLGEYGRLGHGDNTTQLKPKMVKVLLGHRVIQVACGSRDAQTLALTDEGLVFSWGDGDFGKLGRGGSEGCNIPQNIERLNGQGVCQIECGAQFSLALTKSGVVWTWGKGDYFRLGHGTDVHVRKPQVVEGLRGKKIVHVAVGALHCLAVTDTGQVYAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSVAWTTVDVATPSVHEPVLFQTARDPLGASYLGVPSDADSSAASNKISGVNSSKPNRPSLAKILLSLDGNLAKQQALSHILTALQIMYARDAVVGALMPASMIAPVECSSSSSPAPPSDVSSAGSPMNGEESVLAVDVEDRLSPNPWQDKRGEVVSSEDAVTPSAVTPSASSASSRPFIPVTDDPGAASIIAETMTKTKEDVESQNKVSGPEPQYLDEFTSLLVPDDTRVMVDLLKLSVSNRAGEKGKDVLSAVLSGMGTAYPQVADMLLELCVTELEDVATDSQSGRLSSQPVVVESSHPYTDDTSTSGTVKIPGAEGLRVEFDRQCSTERRHDPLTIMDGVNRIVSVRSGREWSDWSSELRIPGDELKWKFISDGSVNGWGWRFTVYPIMPAAGPKDLLSDRCILSCPSMDLVTCLLDFRLNLASNRSIVPRLAASLAACAQLSALAAGHRMWALQRLRKLLTTEFGQSININRILGDNDGETRTMSFTGSALAALVKGLPEALQRQFEYEDPIVRGGKQLLHSPFFKVLVALACDLELDTLPCCAETHKWAWFRRYCMASRVSVALDKRTPLPRLFLDEVAKKIRELMADSENLDVLHESHDIFKREQDEQLVQWMNRRPDDWTLSAGGSGTIYGWGHNHRGQLGGIEGAKVKVPTPCEALAALRPIQLIGGEQTLFAVTADGKLYATGYGAGGRLGIGGTESVSTPTLLESIQHVFIKKVAVNSGGKHCLALSSEGEVYSWGEAEDGKLGHGNRSPCDRPRVIESLRGIEVVDIAAGGAHSACITAAGDLYTWGKGRYGRLGHGDSEDQLKPKLVEALQGYRVIDIACGSGDAQTLCLTDDDTVWSWGDGDYGKLGRGGSDGCKVPMKIDSLTGLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLGHGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCTEDGEVYTWGDNDEGQLGDGTTNAIQRPRLVAALQGKKINRVACGSAHTLAWSTSKPANAGKLPTQVPMEYNHLQEFLIIPLRNRLLLLHHISELFCPCIPMFDLEGRLDETGQGPSVGFDTLRGILISQGKEAAFRKVVQATMVRDRQHGPVVELNRIQVKRSRSKGGLAGPDGTKSVFGQMCAKMISFSPDSLLLPHRVWKVKFVGESVDDCGGGYSESIAEMCEELQNGLTPLLIVTPNGRDESGANRDCFLLNPAARSSLHTNMFRFLGVLLGIAIRTGSPLSLNLAEPVWKQLAGMNLTIADLSEVDKDFIPGLMYIRDNEATSEEFEAMSLPFTVPSASGQDIQLSSKYTHITLDNRAEYVRLAINYRFVLDKYNPPDHFLPESYTCFFLLKLPRYSCKQVLEEKLKYAIHFCKSIDTDDYARIALTGEPAADDSSDDSDNEDADSFASDSTQDYLTGH

Target ORF information:

RefSeq Version	XM_012546136.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii E3 ubiquitin-protein ligase HERC2 (LOC100915156), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012546136.1

ORF Insert Sequence:

ATGTCTTCTG AATCCTTCTG TTTAGCTGCC CAGGCTAGAT TTGACTCCAA GTGGTTAAAA 
ACAGATTTAC AGCTTGCTTT TACAAGAGAT GGACTCTGTG GCCTATGGAA TGAAATGGTC 
AAAGATGGAG AAATTGTATA TCCTGGGACA GATGTAATAC AGAATGGAGA ACTACCTCCT 
CGAAAAGATG ACACCATGGA ATGTCAGAGT GGAACAAAGA AAGAAGATCA AAATGATAAA 
GAGAAGAAAG ATGAAGAGGA AATTCCAGTC CCTATGTATA AGGCCAAATC ACTTGTGGAG 
AGTTGGGTAT GGGGCAAACA ACCAGATGTG AACGAGTTGA AAGAGTGCCT TTATGTGCTA 
GTGAAAGAAC AGCAAACTCT TGCTACACGG ATGGCTACAA CAACTCTTTC AGCAGTAAGG 
CTAAAGCAGA GGCTGGTTAT ACTGGAACGA TATTTCATTG CACTGAATAG AACTGTTCTT 
CAGGAAAATG TCAAAGTTAA GTGGAAAAGC AACAGTATTC CTTTACCTAC TGTCGACAAA 
AAAAGTGATG TTCATGGAAC TGCAAGTACC AAAGGACCAG GAAACATTCC ATTACAAGAC 
CAGCACTTAG CACTTGCCAT ATTGTTAGAG TTGGCTGTGC AGAGAGGCAC TCTAAGCCAA 
ATGTTATCTG CCATTATGTT ACTACTTCAA TTATGGGACA ATGGGACAAG GGAAACAGAT 
AATGAACGAT CTGCCCAAGG AACTAGTGCA CCACTTCTCC CTTTATTACA AAGATTTCAG 
AGTATAATCT GCAATAAAGA CATACTTAAT ACTGAAGATG AGATTCAGAT GCTGTCTGGT 
CTTCTGAGTC CCAATGAAAG TTTTTTGAGG TACCTTACTT TACCACAAGA CAATGAATTA 
GCCATTGATT TGCGTCAAAC TGCAGTAGTG ATTATGGCGC ATTTAGATCG TCTGGCCACT 
CCCTGTATGC CTCCTCAGTG TAGTTCTCCA ACATCTCATA AGGGATCTTT ACAAGAAGTC 
ATAGGTTGGG GCTTAATAGG ATGGAAATAC TATGCTAATG TGATTGGCCC AATTCAATGT 
GAAGGACTTG CAAATCTTGG GATCACACAG ATTGCCTGTG CAGAAAAGCG TTTCCTGATT 
TTGTCTCGGA ATGGGCGAGT TTACATGCAG GCTTACAACA GTGACACATT GGTAGGGGTG 
GTTGCTGTTT TTTCCCTAAT TTTTGGTCAA GTCTGGTCTT GGGGCGATGG TGATTATGGA 
AAACTAGGAA GAGGTGGTAG TGATGGTTGT AAAACACCAA AACTGATTGA AAAACTTCAA 
GACTTAGATG TTGTTAAAGT ACGTTGTGGA AGCCAATTTT CCGTTGCTTT AACAAAAGAT 
GGTCAAGTTT ACTCGTGGGG AAAAGGTGAC AATCAGAGAC TTGGGCATGG AACAGAGGAG 
CATGTTCGAT ATCCGAAACT GTTAGAAGGC TTACAAGATA CAAAACATAT AGTTGGAATT 
GCTTGTGGGC CCGCTCAGAG TTTTGCTTGG TCTTCTTGTT CTGAGTGGTC CATTGGCCTA 
CGTGTACCTT TTGTGGTGGA TGTTTGTCCC ATGACATTTG AACAGTTGGA TCTCTTACTA 
CGACAAGTAA GTGAGGGAAT GGATGGCACC TCTGACTGGC CACCACCACA GGAGAAAGAA 
TGTATGGCAG TGGCAACATT GAACCTTCTG AGACTTCAGT TGCATGCTGC TATTAGTCAC 
CAGGTTGATC CGGAATGTCT TGGATTGGGT CTGGGAAGTG TACTCTTGAA TAGCTTGAAG 
CAGACAGTTG TAACACTAGC AAGTAATGCT GGAGTTCTTA ATACAGTGCA ATCAGCTGCT 
CAAGCAGTAC TGCAGAGTGG ATGGTCTGTG CTGCTTCCAA CTGCAGAGGA ACGAGCAAGG 
GCTCTTTCAG CTCTCTTACC CAGTGCAGTT TCAGGAAATG ACATGAATGT AAGTCCAGGA 
CGTCGTTTTA TGATTGATCT TCTTGTAGGC AGTTTGATGG CTGATGGAGG ATTAGAATCA 
GCATTAAATG CTGCCATCAC TGCAGAAATC CAGGATATTG AAGCAAAAAA AGAAGCTCAA 
AAGGAAAAAG AGATTGATGA GCAGGAAGCA AATGCTTCAA CACTTCATCG GAGTAGGACA 
CCATTAGATA AAGACCTCAT TAATACTGGA ATTTATGAAT CTGCCGGAAA ACAGAGTTTG 
CCTCTGGTTC AATTAATACA ACAGCTTCTT AGAAATATTG CTTCCCAGAC AATAGCCAAA 
TTGAAAGATG TTGCTCGTCG AATTTTGTCC TGTATAGAAC ATGATCAATA TAGTCAAGAC 
AGATCTGCCT CCCTGGATTT GTTGCTTCGT TTTCAGCGTC TGCTGGTCAG TAAACTCTAT 
CCAGGAGACA GTGGTGGTCA AGTTTCAAAT TCCTTTAGTC CTGAGCTATT AGGAGTTGGT 
TCTTTGCTGA AAAAATATAC AGCTCTTTTG TGCACACACA TTGGAGATAT ATTGCCTGTG 
GCAACTAGTA TTGCATCTTC CAGTTATCGG CATTTTGCAG AAGTATCTCG TGTTGTGGAT 
GGTGATCTAA CAGGTGTTCT TCTTCCAGAG TTGATAGTAT CTATTGTCCT TTTGCTCAAT 
AAAAGTCCTG GATTAATGCA GGAAGCTGGG GCAATTCCTC TCTTAGCTGG TCTACTGGAA 
CATCTAGATA GATTTAACCA TTTGGCACCA GGAAAAGAGA GAGATGATAA TGAGGATTTA 
GCCTGGCCTG GAATAATGGG ATCATTTTTT ACAGGTCAGA ATAGTAGAAA TAATGAGGAA 
GTGACGCTCA TACGTAAAGC TGATCTGGAG AATCACAATA AAGATGGAGG CTTCTGGACT 
GTGATTGACG GGAAAGTGTA TGATATAAAA GACTTTCAGA CACAGTCTTT AACAGGCAAT 
AGCATTCTTG CTCAGTTTGC AGGTGATGAC CCGGTGGTTG CTTTGGAAGC TGCTTTACAG 
TTTGAGGACA CAAGGGATTC AATGCATGCA TTTTGTGTTG GCCAGTATAT GGAGCCTGAC 
CAGGAAGTGA TTACAACGCC AGACCTTACC AGTCTCTCTT CACCTCTCAT AGATCCAGAA 
AGAAATCTGG GTCTCCTCCT TGGACTTCAT GCTTCCTATT TAGCAATGAG CACACCACTA 
TCTCCTGTGG AGATTGAATG TGCCAAATGG CTTAAGTCAT CCATATTCTC TGGAGGATTG 
CAAACCAGTC AGATACATTA TAGTTACAAT GAAGAGAAAG ATGAAGACCA CTGCAGTTCT 
CCTGGAAATA CTACTCCAAA CAAATCCAAA CTCTATTCCC ATAGATTGAC TTTGAGTGAT 
CATTCCCAAC CATTTTTGCA AGCCATTGCA GATAATAACA TTCAGGATCA TACCGTGAAG 
GATTTTTTAT GTCAGATAGA AAGGTATTGC AAGCAATGCC ATTTGACCAC ACCGATAACA 
TTTCCTCCTG AACATCCTGT GGAAGAAGTG GGACGTTTAC TCTTATGTTG TCTTCTAAAG 
CATGAAGATT TAGGTCATGT GGCCTTATCT TTAGTTCACC CAGTTACACT TGGTGTTGAC 
CAAGTCAAAC ACAGAACGTT GCCTAAGTCT GTAGTGGATG TTTGTAGAGT CGTCTACCAG 
GCAAAATGCT CACTAATAAA GACACATCAG GAACAGGGAC GTTCTTATAA GGAAGTGTGT 
GCTCCTGTTA TTGAACGTTT GCGATTTCTT TTTAATGAAT TGCGTCCTGC AGTCTGCAAT 
GACCTCTCAA TAATGTCCAA ACTTAAACTT TTAAGCTCTT TGCCTCGTTG GAGAAGAATA 
GCTCAGAAGA TGATACGAGA GAGAAGGAAA TTAAAAAGAG TACCCAAAAA GTCAGAACCT 
ACTGATGTGG AAGAATCTAA GATTGGCAAT GAAGAGGGTG ACTTAGAAGA AGCCTGTATT 
CTTCCTCATA GTCCTGTATC TGTAGATAAA AGACCTGTTC CAGTTAAATC ACCTAAGGAT 
AAATGGCAGC CACTTCTGAG TACAGTTACA GGTGTTCACA AATACAAATG GCTTAAACAG 
AATGTTCAAG GCCTTTATCC TCAGTCTGCA CTGCTTAGCA CAATTGTTGA ATTTGCGCTT 
AAAGAAGAGC CAGTTGATGT GGAAAAGATG AGAAAGTGCT TACTCAAACA GTTGGAGAGA 
GCAGAAGTTC GTTTGGAAGG AATAGACACG ATTTTAAAAT TGGCAAATAA AAATTTCTTG 
CTTTCGTCTG TGCAATATGC TATGTTCTGT GGATGGCAAA GACTTATCCC AGAAGGAACC 
AATATAGGGG AACCACTTAC AGATTGTTTA AAGGATGTTG ATCTGATTCC ACCATTTAAT 
CGAATGCTAC TAGAAGTAAC TTTTGGAAAG TTGTATGGTT GGGCCATTCA GAATATTCGA 
AATGTTTTGT TGGATGCAAA TGCCAGATTT AAAGAGCTAG GTATCCAGCC TGTTCCTCTG 
CAAACTATTA CTAATGAGAA TCCAGCAGGG CCAAGCCTTG GAACCATCCC ACAAGCACGT 
TTTCTCCTGG TTATGCTCAA CATGCTTACC CTTCAATATG GTGCAAACAA CTTGAATCTT 
CTTTTGAACT CTGGCATGCT AGCATTGACT CAGACAACAT TGCGATTAAT AGGTCCTAGT 
TCTGATAATA TCGAGGAAGA AATGAATGCC TCTGCACGAG GAGCTTCAGC TACTGTTTTG 
GAAGAATCCA GAAAAGAAAC CACTCCAGTA CAACTTCCTG TTTCAGGGCC AGAACTGGCA 
GCAATGATGA AAATTGGTAC CAGGGTCATG AGGGGAGTGG ACTGGAAATG GGGAGATCAG 
GATGGTCCAC CTCCAGGTTT AGGTCGTGTG ATTGGTGAAT TGGGAGAGGA TGGATGGATA 
AGGGTTCAGT GGGATACAGG CAGTACTAAT TCATACCGAA TGGGAAAGGA AGGAAAGTAT 
GATCTTAAAT TGGCTGAGCC TCCACCTGCA GCTCAGCCCT CAACAGAGGA TTCGGACACT 
GAGGATGATT CTGAAAGTGA ACAAATGGAA AGAAACTTCC ACCCTACTTC TATTATGCTT 
ACAAGTACTG TTAATTTACT CCAAACTCTC TGTCTCTGTG CTGGAATTCA TGCAGAAATC 
ATGCAAAATG AAGCTACAAG AACTCTGTGT GGTCTACTTA GAATGTTAGT GGAAAGTGGA 
ACAGTAGATA AAACATCTTC TTTACCAAAT AAGATGGTTT ATAAAGAGCA GCACAGAAGT 
TGGTGTACAC TTGGATTTAT TCGAAGTATA GCACTTATGC CTCAAATGTG TAGCACCCTT 
AGCACATCTC AGTGGATCAC ATTACTAATG AAAATTGTGG AAGGACACGA GTCATTTACA 
GCAGCTTCGC TTCAGAGACA GATCTTAGCA GTACATTTAC TACAAGCTGT ACTTCCATCT 
TGGGACAAAA CAGAAAGATC AAGAGATATG AAGTTTCTTG TGGAAAAACT TTTTGGCTTC 
TTGGGCAGTC TACTTAGTAC ATGCTCTTCA GACATACCTT TGCTCAGAGA ATCAACTCTG 
AGAAGGAGGA AGTCCAGACC CCAGGCATCT TTGACTGCAA CCCATAGTAG TACTTTGGCA 
GAGGAGATAG TGGCCCTGCT AAGGACTCTA CATTCACTGA GCCAGTGGAA TGGACTCATA 
AACAAGTACA TCAACTCTCA GCTAAACTCC ATCACCCACA TCTTTGCAGG AAAACAGTCT 
GAAGGGACTG TGTTAGAAGA TTACTTTCCT GATTCTGAGA ACCCTGAAGT TGATGGCCTA 
ATGGCAGTGC TAGCTGTAAT TGGTGGAATA GATAGTCGAT TGAGGCTGGG TGGCCAAGTG 
GTACATGATG AATTTGGAGA AGGCACAGTG ACACGCATCA CTCCTAAAGG CAAAATCACA 
GTCCAGTTCT GTGATATGCG AATATGTAGA ATTTGTCCAT TGAGTCAGCT AAAACCGCTT 
CCAGTTATGG CATTTAATGT GAACAACTTA CCATTCACGG AATCTATGCT CTCTGTCTGG 
GCTCAATTGG TCAACTTGGC AGGGAGCAAA TTAGAAAAAC ACAGAATTAA GAAATCTACT 
AATCAAGCCC TCACAGGACA AGTAGACTTG GATTTGCTAA GATGCCAACA GTTGAAATTA 
TACATATTGA AAGCAGGTCG AGCATTGCTT TCCCATCAGG ATAAACTGAG GCAGATCTTA 
TCTCAACCAG CTTTACAAGA GAATGGAGTT CTCTCTACAG ATGATGGTAC AGCATCCCCT 
GATATTGGAG ATATGTCTCC TGAAGGACCT CAACCTCCAA TGATCCTTTT ACAGCAATTG 
TTGACATCTG CCACACAGCC ATCACCTGTA AAAGCAATAT TTGACAAACA GGAACTTGAG 
GCTGCTGCTT TGGCTGTATG CCAGTGTCTA GCTGTGGAGT CAACTCATCC ATCAACTCCA 
GTGTTTGAAG AATGTAGCTC TAGTGAGGCC ACAACACCTG TCACAGTCCA GCATATTCGA 
TCTGTAAAAG TGAAAAAGCG AAAGCAGTCT CCTCTCCCTG CTTTGCCTAT TGTAGTACAG 
CTCATGGAAA TGGGATTTCC GAGGAAAAAC ATTGAATTTG CATTGAAGTC TCTTACTGGG 
AGTGCTTCAG GGTTACCAGG TGTTGAAGCC TTGGTTGGTT GGTTGCTGGA TCATCCTGAT 
GTCCAAATTA CTGATCTCTC AGATGCTGAT ACAGTCTCTG ATGAATACTC TGATGATGAA 
ATAGTGGAAG ATGTGGAAGA GACTGAAGCA TCTTACCCTG TGTCTAGTTG TGCTGTTGTA 
ACGGAGAGTC ACACTTATAA AAAACGAGCT GATTTCTTAA GTAATGATGA CTATGCAGTA 
TATGTTAGAG AAAACATTCA GGTAGGAATG ATGGTTAGGT GCTGCAGAAC GTATGAAGAA 
GTTTGTGAAG GAGATGTAGG TAAAGTGATT AAATTGGACA GAGATGGATT ACACGACCTC 
AATGTGCAGT GTGATTGGCA ACAAAAGGGG AGTACCTATT GGGTTAGGTA TATCCATGTT 
GAACTTATAG GTTTCCCACC ACAGAGTTCT CCTTCCCATA TCAAGATTGG TGATAAAGTT 
AGGGTGAAAA CATCAGTAAC CACACCAAAA TATAAATGGG GATCAGTTAC TCATAGAAGT 
GTGGGAGTTG TGAAAGCTTT TAGTGCCAAT GGAAAAGATG TTATTGTGGA TTTTCCTCAG 
CAGTCTCATT GGACAGGATT ATTGTCAGAA ATGGAATTGG TGCCTAGTAT TCATCCTGGA 
GTTACGTGTG ATGGCTGCCA AATGTTTCCT ATCAATGGTC CTCGTTTCAA ATGCAGGAGT 
TGTGATGATT TTGACTTTTG TGAGACCTGT TTTAAAACCA AGAAGCATAA TACCAGACAT 
AGTTTTGGCA GAATAAATGA ACCAGGTCAG TCTCTTGTAT TTTGTGGGCG TTCTGGGAAA 
CAGCTAAAAC GTTGCCACAG TAGTCAGCCT GGAATGCTCT TGGATAATTG GTCAAGAATT 
GTTAAGAATT TAAATGTGTC CTCATCAGTA AATCAGGCTT CTCGCTTGAT TGATGGCAGT 
GAACCATGTT GGCAGTCATC TGGATCACAA GGAAAGCATT GGATTCGACT GGAGATTTTC 
CCCGATGTTC TTGTTCATAG ACTGAAAATG ATAGTAGATC CTGCTGACAG TAGCTACATG 
CCTTCACTAG TTGTAGTTTC AGGTGGGAAT TCCCTAAATA ATTTAATTGA ACTGAAGACA 
ATCAATATTA ACCCAGCTGA TACCACAGTG CCTCTTCTAA ATGACTGCAC AGAGTATCAC 
AGGTATATTG AGGTTGCCAT AAAGCAATGC AGAAGCTCAG GAATAGATTG TAAAATTCAT 
GGTCTTATTA TACTGGGACG AATTCGGGCA GAAGATGAAG ATTTGGCTGC TGTTCCTTTC 
TTAGCGTCAG ATAATGAAGA AGAAGATGAT GAAAAAGCTA ACACTGGGAG TCTTATTAGG 
AAGAAGGCTT CTGGATTGGA ATCAGCAGCC ACAATAAGGA CCAAAGTTTT TGTCTGGGGG 
CTGAATGACA AGGATCAATT AGGAGGATTA AAGGGTTCTA AGATAAAAGT TCCTTCATTC 
TCTGAAACCC TTTCTGCTTT GAATGTAGTT CAAGTGGCAG GTGGATCTAA AAGTTTGTTT 
GCAGTGACTG TGGAGGGTAA AGTGTATGCT TGTGGAGAAG CTACTAATGG ACGACTGGGT 
TTAGGTATTT CTAGTGGAAC TGTACCCATT CCGCGGCAGA TTACAGCTCT CAGCAATTAT 
GTGGTAAAGA AAGTTGCTGT TCACTCAGGT GGCCGGCATG CTATGGCTTT AACTGTTGAT 
GGAAAGATAT TTTCATGGGG TGAAGGTGAT GATGGAAAAC TAGGACACTT CAGCAGAATG 
AACTGTGATA AACCAAGACT GATAGAGGCA TTGAAAACCA AACGTATTCG TGATATAGCT 
TGTGGTAGTT CTCACAGTGC TGCCATTACA TCAAGTGGTG AATTGTATAC ATGGGGCCTT 
GGAGAATATG GCCGGTTGGG ACATGGGGAT AATACTACAC AGCTGAAACC CAAAATGGTG 
AAAGTTCTTC TTGGTCATCG AGTTATTCAA GTTGCTTGTG GAAGTAGAGA TGCCCAGACT 
CTGGCTTTGA CAGATGAAGG TTTGGTCTTT TCCTGGGGAG ATGGTGACTT TGGGAAACTG 
GGCCGGGGAG GAAGTGAAGG ATGTAACATT CCTCAGAACA TTGAGAGACT TAATGGACAA 
GGAGTTTGCC AGATTGAGTG TGGGGCTCAA TTTTCCTTGG CACTTACAAA GTCAGGAGTA 
GTATGGACTT GGGGTAAAGG AGATTACTTT AGGCTTGGTC ATGGCACTGA TGTTCATGTG 
AGAAAACCAC AAGTAGTAGA AGGACTACGA GGAAAGAAGA TAGTTCATGT GGCAGTAGGC 
GCTCTTCACT GCTTAGCTGT CACTGATACA GGTCAAGTGT ATGCTTGGGG TGACAATGAT 
CATGGACAAC AAGGCAATGG GACTACTACT GTCAACAGAA AGCCTACCCT TGTACAAGGC 
TTAGAAGGAC AAAAAATCAC TCGGGTAGCT TGTGGCTCAT CTCATAGTGT GGCGTGGACA 
ACAGTGGATG TAGCAACCCC ATCTGTTCAT GAACCTGTCC TCTTTCAAAC TGCTAGGGAC 
CCTTTAGGCG CTTCTTACTT AGGTGTTCCT TCCGATGCTG ATTCTTCTGC TGCCAGTAAC 
AAAATCAGTG GTGTAAATAG TTCCAAGCCT AACCGCCCTT CCCTTGCTAA GATTCTCTTG 
TCTTTGGATG GAAACCTTGC CAAACAGCAA GCATTATCTC ATATACTTAC AGCATTGCAA 
ATTATGTATG CGAGAGATGC AGTAGTTGGA GCATTAATGC CTGCTAGTAT GATCGCACCA 
GTGGAATGCT CATCTTCTTC ATCACCTGCA CCCCCCTCAG ATGTGTCTTC AGCAGGAAGT 
CCCATGAATG GTGAAGAGTC TGTGTTAGCT GTGGATGTAG AAGATAGACT AAGCCCAAAT 
CCATGGCAAG ACAAGAGAGG GGAGGTAGTT TCTTCAGAAG ATGCTGTGAC TCCATCAGCA 
GTGACTCCCT CAGCTTCCTC TGCCTCTTCT CGACCATTTA TTCCAGTAAC TGATGATCCT 
GGAGCTGCCA GTATCATTGC AGAAACCATG ACTAAAACCA AAGAAGATGT TGAAAGCCAA 
AATAAAGTAT CAGGTCCGGA ACCTCAATAT TTGGATGAAT TTACTAGTCT CTTAGTGCCA 
GATGATACCC GTGTCATGGT TGACTTGCTC AAGCTGTCTG TTTCTAATCG AGCTGGTGAA 
AAGGGAAAAG ATGTTCTTTC AGCTGTGCTT TCTGGTATGG GCACTGCTTA TCCACAGGTA 
GCTGATATGT TGTTGGAGCT CTGTGTTACA GAGTTAGAAG ATGTTGCCAC AGACTCTCAG 
AGTGGTCGAC TCTCTTCCCA ACCTGTTGTA GTAGAAAGTA GTCATCCGTA TACTGATGAT 
ACCTCTACCA GTGGTACTGT CAAAATACCA GGTGCTGAAG GACTCAGAGT AGAGTTTGAT 
CGACAATGTT CTACAGAACG GCGTCATGAC CCTCTCACAA TCATGGATGG TGTCAATAGG 
ATAGTTTCAG TTCGATCAGG TCGAGAATGG TCTGATTGGT CCAGTGAACT TCGAATTCCT 
GGAGATGAAT TAAAGTGGAA GTTCATCAGT GATGGATCAG TGAATGGATG GGGTTGGCGA 
TTCACTGTCT ATCCTATTAT GCCAGCTGCA GGCCCTAAAG ATCTTCTTTC AGATCGGTGT 
ATTCTTTCCT GTCCCTCAAT GGACTTGGTT ACATGCTTGT TGGACTTTAG ATTAAATTTA 
GCTTCCAATA GAAGTATTGT TCCTCGTTTG GCAGCATCAC TTGCAGCATG TGCTCAATTG 
AGTGCTCTAG CTGCTGGTCA CAGAATGTGG GCCCTTCAAA GACTGAGGAA ACTGCTTACA 
ACTGAATTTG GACAATCAAT CAATATAAAT AGGATACTTG GAGATAATGA TGGAGAAACA 
AGAACCATGA GTTTTACCGG TAGTGCTTTA GCAGCTTTGG TAAAAGGTCT TCCAGAAGCT 
TTGCAAAGAC AGTTTGAATA TGAAGATCCG ATTGTTAGAG GAGGCAAACA ACTGCTCCAT 
AGCCCTTTCT TTAAGGTGCT GGTAGCTCTT GCTTGTGACC TAGAACTGGA TACTCTTCCT 
TGCTGTGCTG AGACACACAA ATGGGCTTGG TTTAGAAGAT ACTGTATGGC CTCTAGAGTT 
TCAGTAGCCT TGGACAAACG AACACCATTA CCTCGCCTTT TCCTTGATGA GGTGGCAAAG 
AAAATCAGGG AGTTAATGGC CGACAGTGAA AATCTGGATG TTCTGCATGA GAGCCATGAC 
ATTTTTAAGA GAGAACAAGA TGAACAACTC GTCCAGTGGA TGAACAGGAG ACCTGATGAC 
TGGACTCTTT CTGCTGGAGG CAGTGGAACA ATTTATGGTT GGGGTCACAA TCATCGAGGA 
CAGCTTGGCG GTATTGAGGG AGCAAAAGTC AAAGTTCCAA CACCTTGTGA AGCTCTTGCA 
GCTCTGCGGC CCATACAGTT AATTGGAGGG GAACAGACTC TCTTTGCGGT AACTGCTGAT 
GGGAAGCTTT ATGCTACAGG GTATGGTGCA GGTGGCAGAT TGGGAATTGG AGGAACTGAA 
TCAGTTTCTA CTCCAACATT GCTTGAATCT ATTCAGCATG TATTTATAAA AAAAGTTGCT 
GTGAACTCTG GAGGAAAACA CTGCTTAGCA CTCTCATCAG AAGGAGAAGT TTATTCTTGG 
GGTGAGGCAG AAGATGGAAA ATTGGGGCAT GGAAACAGAA GCCCCTGTGA TCGCCCACGT 
GTAATTGAAT CTTTGCGAGG AATAGAAGTT GTTGATATTG CTGCAGGTGG AGCCCACAGT 
GCATGTATTA CAGCAGCTGG TGACCTCTAT ACGTGGGGTA AAGGAAGATA TGGTCGCCTA 
GGTCATGGGG ATAGTGAAGA TCAGTTAAAA CCAAAACTGG TGGAAGCTCT CCAAGGCTAT 
CGAGTTATTG ATATTGCCTG TGGCAGTGGA GATGCACAGA CACTTTGCCT TACTGATGAT 
GATACTGTAT GGTCCTGGGG TGATGGGGAC TATGGAAAAC TTGGCAGAGG AGGCAGTGAT 
GGCTGTAAAG TGCCAATGAA GATTGATTCT CTCACAGGTC TTGGAGTGGT TAAAGTAGAG 
TGTGGATCAC AATTTTCTGT TGCACTTACC AAGTCTGGAG CAGTTTATAC TTGGGGTAAA 
GGCGATTATC ACAGGTTGGG TCATGGATCA GACGACCATG TTAGACGGCC TCGGCAAGTG 
CAAGGCCTAC AAGGAAAGAA AGTAATAGCA ATTGCTACTG GTTCTTTACA CTGTGTGTGC 
TGCACTGAAG ACGGTGAAGT TTATACTTGG GGTGACAATG ATGAAGGGCA ACTTGGGGAT 
GGAACAACTA ATGCAATCCA GAGACCTCGG TTGGTGGCAG CACTTCAGGG TAAAAAGATA 
AATAGGGTGG CTTGTGGTTC AGCACACACC TTAGCGTGGT CCACGAGTAA ACCTGCCAAT 
GCTGGGAAGC TTCCAACACA GGTTCCTATG GAATACAACC ATTTACAGGA ATTTCTCATA 
ATTCCTTTAC GAAACAGACT TTTGTTATTA CATCATATCT CAGAACTCTT TTGTCCATGC 
ATTCCCATGT TTGACCTTGA GGGGCGTCTT GATGAAACTG GCCAAGGACC TTCAGTGGGA 
TTTGACACAT TACGAGGAAT TCTGATATCT CAGGGAAAGG AAGCAGCTTT CCGAAAAGTT 
GTACAAGCAA CTATGGTGAG AGATCGTCAA CATGGTCCTG TAGTTGAATT AAACAGAATA 
CAGGTAAAAC GATCAAGAAG TAAGGGTGGT TTGGCTGGTC CTGATGGTAC TAAATCTGTT 
TTTGGCCAAA TGTGTGCTAA AATGATTTCA TTTAGTCCAG ACAGTCTTCT TCTACCTCAT 
CGAGTATGGA AAGTCAAGTT TGTTGGCGAG TCTGTGGATG ATTGTGGAGG TGGTTATAGT 
GAATCAATAG CAGAAATGTG TGAAGAACTT CAGAATGGAC TTACTCCTCT CCTAATTGTA 
ACTCCAAATG GCAGGGATGA ATCTGGAGCC AATAGAGATT GCTTCTTATT GAACCCAGCT 
GCCAGATCTT CTTTGCATAC CAATATGTTT CGTTTCTTGG GTGTATTGCT AGGCATTGCC 
ATTCGAACTG GGAGTCCATT AAGCCTCAAC CTTGCAGAAC CGGTTTGGAA GCAACTGGCA 
GGAATGAACC TTACAATTGC TGATCTCAGT GAGGTTGATA AGGATTTTAT TCCTGGCCTG 
ATGTACATTC GGGACAATGA AGCTACTTCT GAGGAATTTG AAGCAATGAG CCTTCCCTTC 
ACAGTGCCAA GTGCAAGTGG GCAGGATATT CAATTGAGCT CTAAATATAC CCACATCACA 
TTGGATAACC GTGCTGAGTA TGTTAGATTG GCAATAAATT ACAGGTTTGT TTTGGATAAG 
TACAATCCAC CTGACCACTT CCTTCCAGAG TCATATACAT GCTTCTTTTT GCTGAAGTTA 
CCCAGGTATT CCTGTAAACA AGTACTAGAG GAAAAACTGA AATATGCCAT TCATTTTTGC 
AAGTCTATAG ACACTGATGA TTATGCCCGG ATAGCACTCA CAGGAGAACC AGCTGCTGAT 
GACAGCAGTG ATGATTCAGA TAATGAAGAT GCAGATTCAT TTGCATCGGA CTCTACTCAA 
GATTATTTAA CAGGACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC2 ( XM_031959088.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031814948.1 Sarcophilus harrisii HECT and RLD domain containing E3 ubiquitin protein ligase 2 (HERC2), transcript variant X1, mRNA.

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CloneID	OSa152727
Clone ID Related Accession (Same CDS sequence)	XM_031959088.1 , XM_023500395.1
Accession Version	XM_031959088.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 14517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	E3 ubiquitin-protein ligase HERC2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045428.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTCTG AATCCTTCTG TTTAGCTGCC CAGGCTAGAT TTGACTCCAA GTGGTTAAAA 
ACAGATTTAC AGCTTGCTTT TACAAGAGAT GGACTCTGTG GCCTATGGAA TGAAATGGTC 
AAAGATGGAG AAATTGTATA TCCTGGGACA GATGTAATAC AGAATGGAGA ACTACCTCCT 
CGAAAAGATG ACACCATGGA ATGTCAGAGT GGAACAAAGA AAGAAGATCA AAATGATAAA 
GAGAAGAAAG ATGAAGAGGA AATTCCAGTC CCTATGTATA AGGCCAAATC ACTTGTGGAG 
AGTTGGGTAT GGGGCAAACA ACCAGATGTG AACGAGTTGA AAGAGTGCCT TTATGTGCTA 
GTGAAAGAAC AGCAAACTCT TGCTACACGG ATGGCTACAA CAACTCTTTC AGCAGTAAGG 
CTAAAGCAGA GGCTGGTTAT ACTGGAACGA TATTTCATTG CACTGAATAG AACTGTTCTT 
CAGGAAAATG TCAAAGTTAA GTGGAAAAGC AACAGTATTC CTTTACCTAC TGTCGACAAA 
AAAAGTTCTA GACCTATAGG AAAAGGTGTA GAAGGACTTG CAAGAGTTGG ATCCAGAGCA 
GCCCTTTCAT TTGCTTTTGC ATTCCTACGC AGAGCTTGGC GGTCAGGGGA AGATGCCGAT 
CTATGCAGTG AATTGTTACA GGAGTCTCTT GATGCCTTGA GAGCCCTACC AGAAGCATCT 
CTTTTTGATG AAAGCACAGT GTCATCTGTT TGGTTGGAAG TTGTGGAGAG AGCAACTAAA 
TTCCTAAGGT CTGTTGTTAC TGGTGATGTT CATGGAACTG CAAGTACCAA AGGACCAGGA 
AACATTCCAT TACAAGACCA GCACTTAGCA CTTGCCATAT TGTTAGAGTT GGCTGTGCAG 
AGAGGCACTC TAAGCCAAAT GTTATCTGCC ATTATGTTAC TACTTCAATT ATGGGACAAT 
GGGACAAGGG AAACAGATAA TGAACGATCT GCCCAAGGAA CTAGTGCACC ACTTCTCCCT 
TTATTACAAA GATTTCAGAG TATAATCTGC AATAAAGACA TACTTAATAC TGAAGATGAG 
ATTCAGATGC TGTCTGGTCT TCTGAGTCCC AATGAAAGTT TTTTGAGGTA CCTTACTTTA 
CCACAAGACA ATGAATTAGC CATTGATTTG CGTCAAACTG CAGTAGTGAT TATGGCGCAT 
TTAGATCGTC TGGCCACTCC CTGTATGCCT CCTCAGTGTA GTTCTCCAAC ATCTCATAAG 
GGATCTTTAC AAGAAGTCAT AGGTTGGGGC TTAATAGGAT GGAAATACTA TGCTAATGTG 
ATTGGCCCAA TTCAATGTGA AGGACTTGCA AATCTTGGGA TCACACAGAT TGCCTGTGCA 
GAAAAGCGTT TCCTGATTTT GTCTCGGAAT GGGCGAGTTT ACATGCAGGC TTACAACAGT 
GACACATTGG GACCCCAACT AGTTCAAGGT CTTGCCTCTA GAAATATTGT GAAAATTGCA 
GCACATTCAG ATGGTCATCA TTATCTGGCT TTGGCAGCCA CTGGAGAGGT CTTTTCTTGG 
GGCTGTGGAG ATGGTGGACG GTTAGGCCAT GGTGATACAG TGCCTCTAGA AGAACCTAAG 
ATGATATCTG CTCTCTCAGG AAAGCAAGCT GGGAAACATG TAGTTCATAT CGCATGTGGA 
AGTACTTATA GTGCAGCTAT TACAGCTGAA GGAGAGTTAT ATACTTGGGG TCGAGGAAAC 
TATGGCAGGC TGGGACATGG CTCAAGTGAA GATCAGACAA TTCCAATGCT AGTAACTGGA 
CTTAAAGGAC TGAAGGTTAT TGATGTCTCT TGTGGGAGTG GGGATGCTCA GACTCTTGCA 
GTGACTGAGA ATGGTCAAGT CTGGTCTTGG GGCGATGGTG ATTATGGAAA ACTAGGAAGA 
GGTGGTAGTG ATGGTTGTAA AACACCAAAA CTGATTGAAA AACTTCAAGA CTTAGATGTT 
GTTAAAGTAC GTTGTGGAAG CCAATTTTCC GTTGCTTTAA CAAAAGATGG TCAAGTTTAC 
TCGTGGGGAA AAGGTGACAA TCAGAGACTT GGGCATGGAA CAGAGGAGCA TGTTCGATAT 
CCGAAACTGT TAGAAGGCTT ACAAGGTAAA AAAGTCGTTG ATGTGGCTGC TGGTTCTACA 
CACTGCTTAG CACTTACTGA GGATAGCGAA GTTCATAGCT GGGGGAGTAA TGATCAGTGT 
CAGCACTTTG ACACCCTTCG GATTACCAAA CCTGAACCTA CTGCATTGCC AGGATTAGAT 
ACAAAACATA TAGTTGGAAT TGCTTGTGGG CCCGCTCAGA GTTTTGCTTG GTCTTCTTGT 
TCTGAGTGGT CCATTGGCCT ACGTGTACCT TTTGTGGTGG ATGTTTGTCC CATGACATTT 
GAACAGTTGG ATCTCTTACT ACGACAAGTA AGTGAGGGAA TGGATGGCAC CTCTGACTGG 
CCACCACCAC AGGAGAAAGA ATGTATGGCA GTGGCAACAT TGAACCTTCT GAGACTTCAG 
TTGCATGCTG CTATTAGTCA CCAGGTTGAT CCGGAATGTC TTGGATTGGG TCTGGGAAGT 
GTACTCTTGA ATAGCTTGAA GCAGACAGTT GTAACACTAG CAAGTAATGC TGGAGTTCTT 
AATACAGTGC AATCAGCTGC TCAAGCAGTA CTGCAGAGTG GATGGTCTGT GCTGCTTCCA 
ACTGCAGAGG AACGAGCAAG GGCTCTTTCA GCTCTCTTAC CCAGTGCAGT TTCAGGAAAT 
GACATGAATG TAAGTCCAGG ACGTCGTTTT ATGATTGATC TTCTTGTAGG CAGTTTGATG 
GCTGATGGAG GATTAGAATC AGCATTAAAT GCTGCCATCA CTGCAGAAAT CCAGGATATT 
GAAGCAAAAA AAGAAGCTCA AAAGGAAAAA GAGATTGATG AGCAGGAAGC AAATGCTTCA 
ACACTTCATC GGAGTAGGAC ACCATTAGAT AAAGACCTCA TTAATACTGG AATTTATGAA 
TCTGCCGGAA AACAGAGTTT GCCTCTGGTT CAATTAATAC AACAGCTTCT TAGAAATATT 
GCTTCCCAGA CAATAGCCAA ATTGAAAGAT GTTGCTCGTC GAATTTTGTC CTGTATAGAA 
CATGATCAAT ATAGTCAAGA CAGATCTGCC TCCCTGGATT TGTTGCTTCG TTTTCAGCGT 
CTGCTGGTCA GTAAACTCTA TCCAGGAGAC AGTGGTGGTC AAGTTTCAAA TTCCTTTAGT 
CCTGAGCTAT TAGGAGTTGG TTCTTTGCTG AAAAAATATA CAGCTCTTTT GTGCACACAC 
ATTGGAGATA TATTGCCTGT GGCAACTAGT ATTGCATCTT CCAGTTATCG GCATTTTGCA 
GAAGTATCTC GTGTTGTGGA TGGTGATCTA ACAGGTGTTC TTCTTCCAGA GTTGATAGTA 
TCTATTGTCC TTTTGCTCAA TAAAAGTCCT GGATTAATGC AGGAAGCTGG GGCAATTCCT 
CTCTTAGCTG GTCTACTGGA ACATCTAGAT AGATTTAACC ATTTGGCACC AGGAAAAGAG 
AGAGATGATA ATGAGGATTT AGCCTGGCCT GGAATAATGG GATCATTTTT TACAGGTCAG 
AATAGTAGAA ATAATGAGGA AGTGACGCTC ATACGTAAAG CTGATCTGGA GAATCACAAT 
AAAGATGGAG GCTTCTGGAC TGTGATTGAC GGGAAAGTGT ATGATATAAA AGACTTTCAG 
ACACAGTCTT TAACAGGCAA TAGCATTCTT GCTCAGTTTG CAGGTGATGA CCCGGTGGTT 
GCTTTGGAAG CTGCTTTACA GTTTGAGGAC ACAAGGGATT CAATGCATGC ATTTTGTGTT 
GGCCAGTATA TGGAGCCTGA CCAGGAAGTG ATTACAACGC CAGACCTTAC CAGTCTCTCT 
TCACCTCTCA TAGATCCAGA AAGAAATCTG GGTCTCCTCC TTGGACTTCA TGCTTCCTAT 
TTAGCAATGA GCACACCACT ATCTCCTGTG GAGATTGAAT GTGCCAAATG GCTTAAGTCA 
TCCATATTCT CTGGAGGATT GCAAACCAGT CAGATACATT ATAGTTACAA TGAAGAGAAA 
GATGAAGACC ACTGCAGTTC TCCTGGAAAT ACTACTCCAA ACAAATCCAA ACTCTATTCC 
CATAGATTGA CTTTGAGTGA TCATTCCCAA CCATTTTTGC AAGCCATTGC AGATAATAAC 
ATTCAGGATC ATACCGTGAA GGATTTTTTA TGTCAGATAG AAAGGTATTG CAAGCAATGC 
CATTTGACCA CACCGATAAC ATTTCCTCCT GAACATCCTG TGGAAGAAGT GGGACGTTTA 
CTCTTATGTT GTCTTCTAAA GCATGAAGAT TTAGGTCATG TGGCCTTATC TTTAGTTCAC 
CCAGTTACAC TTGGTGTTGA CCAAGTCAAA CACAGAACGT TGCCTAAGTC TGTAGTGGAT 
GTTTGTAGAG TCGTCTACCA GGCAAAATGC TCACTAATAA AGACACATCA GGAACAGGGA 
CGTTCTTATA AGGAAGTGTG TGCTCCTGTT ATTGAACGTT TGCGATTTCT TTTTAATGAA 
TTGCGTCCTG CAGTCTGCAA TGACCTCTCA ATAATGTCCA AACTTAAACT TTTAAGCTCT 
TTGCCTCGTT GGAGAAGAAT AGCTCAGAAG ATGATACGAG AGAGAAGGAA ATTAAAAAGA 
GTACCCAAAA AGTCAGAACC TACTGATGTG GAAGAATCTA AGATTGGCAA TGAAGAGGGT 
GACTTAGAAG AAGCCTGTAT TCTTCCTCAT AGTCCTGTAT CTGTAGATAA AAGACCTGTT 
CCAGTTAAAT CACCTAAGGA TAAATGGCAG CCACTTCTGA GTACAGTTAC AGGTGTTCAC 
AAATACAAAT GGCTTAAACA GAATGTTCAA GGCCTTTATC CTCAGTCTGC ACTGCTTAGC 
ACAATTGTTG AATTTGCGCT TAAAGAAGAG CCAGTTGATG TGGAAAAGAT GAGAAAGTGC 
TTACTCAAAC AGTTGGAGAG AGCAGAAGTT CGTTTGGAAG GAATAGACAC GATTTTAAAA 
TTGGCAAATA AAAATTTCTT GCTTTCGTCT GTGCAATATG CTATGTTCTG TGGATGGCAA 
AGACTTATCC CAGAAGGAAC CAATATAGGG GAACCACTTA CAGATTGTTT AAAGGATGTT 
GATCTGATTC CACCATTTAA TCGAATGCTA CTAGAAGTAA CTTTTGGAAA GTTGTATGGT 
TGGGCCATTC AGAATATTCG AAATGTTTTG TTGGATGCAA ATGCCAGATT TAAAGAGCTA 
GGTATCCAGC CTGTTCCTCT GCAAACTATT ACTAATGAGA ATCCAGCAGG GCCAAGCCTT 
GGAACCATCC CACAAGCACG TTTTCTCCTG GTTATGCTCA ACATGCTTAC CCTTCAATAT 
GGTGCAAACA ACTTGAATCT TCTTTTGAAC TCTGGCATGC TAGCATTGAC TCAGACAACA 
TTGCGATTAA TAGGTCCTAG TTCTGATAAT ATCGAGGAAG AAATGAATGC CTCTGCACGA 
GGAGCTTCAG CTACTGTTTT GGAAGAATCC AGAAAAGAAA CCACTCCAGT ACAACTTCCT 
GTTTCAGGGC CAGAACTGGC AGCAATGATG AAAATTGGTA CCAGGGTCAT GAGGGGAGTG 
GACTGGAAAT GGGGAGATCA GGATGGTCCA CCTCCAGGTT TAGGTCGTGT GATTGGTGAA 
TTGGGAGAGG ATGGATGGAT AAGGGTTCAG TGGGATACAG GCAGTACTAA TTCATACCGA 
ATGGGAAAGG AAGGAAAGTA TGATCTTAAA TTGGCTGAGC CTCCACCTGC AGCTCAGCCC 
TCAACAGAGG ATTCGGACAC TGAGGATGAT TCTGAAAGTG AACAAATGGA AAGAAACTTC 
CACCCTACTT CTATTATGCT TACAAGTACT GTTAATTTAC TCCAAACTCT CTGTCTCTGT 
GCTGGAATTC ATGCAGAAAT CATGCAAAAT GAAGCTACAA GAACTCTGTG TGGTCTACTT 
AGAATGTTAG TGGAAAGTGG AACAGTAGAT AAAACATCTT CTTTACCAAA TAAGATGGTT 
TATAAAGAGC AGCACAGAAG TTGGTGTACA CTTGGATTTA TTCGAAGTAT AGCACTTATG 
CCTCAAATGT GTAGCACCCT TAGCACATCT CAGTGGATCA CATTACTAAT GAAAATTGTG 
GAAGGACACG AGTCATTTAC AGCAGCTTCG CTTCAGAGAC AGATCTTAGC AGTACATTTA 
CTACAAGCTG TACTTCCATC TTGGGACAAA ACAGAAAGAT CAAGAGATAT GAAGTTTCTT 
GTGGAAAAAC TTTTTGGCTT CTTGGGCAGT CTACTTAGTA CATGCTCTTC AGACATACCT 
TTGCTCAGAG AATCAACTCT GAGAAGGAGG AAGTCCAGAC CCCAGGCATC TTTGACTGCA 
ACCCATAGTA GTACTTTGGC AGAGGAGATA GTGGCCCTGC TAAGGACTCT ACATTCACTG 
AGCCAGTGGA ATGGACTCAT AAACAAGTAC ATCAACTCTC AGCTAAACTC CATCACCCAC 
ATCTTTGCAG GAAAACAGTC TGAAGGGACT GTGTTAGAAG ATTACTTTCC TGATTCTGAG 
AACCCTGAAG TTGATGGCCT AATGGCAGTG CTAGCTGTAA TTGGTGGAAT AGATAGTCGA 
TTGAGGCTGG GTGGCCAAGT GGTACATGAT GAATTTGGAG AAGGCACAGT GACACGCATC 
ACTCCTAAAG GCAAAATCAC AGTCCAGTTC TGTGATATGC GAATATGTAG AATTTGTCCA 
TTGAGTCAGC TAAAACCGCT TCCAGTTATG GCATTTAATG TGAACAACTT ACCATTCACG 
GAATCTATGC TCTCTGTCTG GGCTCAATTG GTCAACTTGG CAGGGAGCAA ATTAGAAAAA 
CACAGAATTA AGAAATCTAC TAATCAAGCC CTCACAGGAC AAGTAGACTT GGATTTGCTA 
AGATGCCAAC AGTTGAAATT ATACATATTG AAAGCAGGTC GAGCATTGCT TTCCCATCAG 
GATAAACTGA GGCAGATCTT ATCTCAACCA GCTTTACAAG AGAATGGAGT TCTCTCTACA 
GATGATGGTA CAGCATCCCC TGATATTGGA GATATGTCTC CTGAAGGACC TCAACCTCCA 
ATGATCCTTT TACAGCAATT GTTGACATCT GCCACACAGC CATCACCTGT AAAAGCAATA 
TTTGACAAAC AGGAACTTGA GGCTGCTGCT TTGGCTGTAT GCCAGTGTCT AGCTGTGGAG 
TCAACTCATC CATCAACTCC AGTGTTTGAA GAATGTAGCT CTAGTGAGGC CACAACACCT 
GTCACAGTCC AGCATATTCG ATCTGTAAAA GTGAAAAAGC GAAAGCAGTC TCCTCTCCCT 
GCTTTGCCTA TTGTAGTACA GCTCATGGAA ATGGGATTTC CGAGGAAAAA CATTGAATTT 
GCATTGAAGT CTCTTACTGG GAGTGCTTCA GGGTTACCAG GTGTTGAAGC CTTGGTTGGT 
TGGTTGCTGG ATCATCCTGA TGTCCAAATT ACTGATCTCT CAGATGCTGA TACAGTCTCT 
GATGAATACT CTGATGATGA AATAGTGGAA GATGTGGAAG AGACTGAAGC ATCTTACCCT 
GTGTCTAGTT GTGCTGTTGT AACGGAGAGT CACACTTATA AAAAACGAGC TGATTTCTTA 
AGTAATGATG ACTATGCAGT ATATGTTAGA GAAAACATTC AGGTAGGAAT GATGGTTAGG 
TGCTGCAGAA CGTATGAAGA AGTTTGTGAA GGAGATGTAG GTAAAGTGAT TAAATTGGAC 
AGAGATGGAT TACACGACCT CAATGTGCAG TGTGATTGGC AACAAAAGGG GAGTACCTAT 
TGGGTTAGGT ATATCCATGT TGAACTTATA GGTTTCCCAC CACAGAGTTC TCCTTCCCAT 
ATCAAGATTG GTGATAAAGT TAGGGTGAAA ACATCAGTAA CCACACCAAA ATATAAATGG 
GGATCAGTTA CTCATAGAAG TGTGGGAGTT GTGAAAGCTT TTAGTGCCAA TGGAAAAGAT 
GTTATTGTGG ATTTTCCTCA GCAGTCTCAT TGGACAGGAT TATTGTCAGA AATGGAATTG 
GTGCCTAGTA TTCATCCTGG AGTTACGTGT GATGGCTGCC AAATGTTTCC TATCAATGGT 
CCTCGTTTCA AATGCAGGAG TTGTGATGAT TTTGACTTTT GTGAGACCTG TTTTAAAACC 
AAGAAGCATA ATACCAGACA TAGTTTTGGC AGAATAAATG AACCAGGTCA GTCTCTTGTA 
TTTTGTGGGC GTTCTGGGAA ACAGCTAAAA CGTTGCCACA GTAGTCAGCC TGGAATGCTC 
TTGGATAATT GGTCAAGAAT TGTTAAGAAT TTAAATGTGT CCTCATCAGT AAATCAGGCT 
TCTCGCTTGA TTGATGGCAG TGAACCATGT TGGCAGTCAT CTGGATCACA AGGAAAGCAT 
TGGATTCGAC TGGAGATTTT CCCCGATGTT CTTGTTCATA GACTGAAAAT GATAGTAGAT 
CCTGCTGACA GTAGCTACAT GCCTTCACTA GTTGTAGTTT CAGGTGGGAA TTCCCTAAAT 
AATTTAATTG AACTGAAGAC AATCAATATT AACCCAGCTG ATACCACAGT GCCTCTTCTA 
AATGACTGCA CAGAGTATCA CAGGTATATT GAGGTTGCCA TAAAGCAATG CAGAAGCTCA 
GGAATAGATT GTAAAATTCA TGGTCTTATT ATACTGGGAC GAATTCGGGC AGAAGATGAA 
GATTTGGCTG CTGTTCCTTT CTTAGCGTCA GATAATGAAG AAGAAGATGA TGAAAAAGCT 
AACACTGGGA GTCTTATTAG GAAGAAGGCT TCTGGATTGG AATCAGCAGC CACAATAAGG 
ACCAAAGTTT TTGTCTGGGG GCTGAATGAC AAGGATCAAT TAGGAGGATT AAAGGGTTCT 
AAGATAAAAG TTCCTTCATT CTCTGAAACC CTTTCTGCTT TGAATGTAGT TCAAGTGGCA 
GGTGGATCTA AAAGTTTGTT TGCAGTGACT GTGGAGGGTA AAGTGTATGC TTGTGGAGAA 
GCTACTAATG GACGACTGGG TTTAGGTATT TCTAGTGGAA CTGTACCCAT TCCGCGGCAG 
ATTACAGCTC TCAGCAATTA TGTGGTAAAG AAAGTTGCTG TTCACTCAGG TGGCCGGCAT 
GCTATGGCTT TAACTGTTGA TGGAAAGATA TTTTCATGGG GTGAAGGTGA TGATGGAAAA 
CTAGGACACT TCAGCAGAAT GAACTGTGAT AAACCAAGAC TGATAGAGGC ATTGAAAACC 
AAACGTATTC GTGATATAGC TTGTGGTAGT TCTCACAGTG CTGCCATTAC ATCAAGTGGT 
GAATTGTATA CATGGGGCCT TGGAGAATAT GGCCGGTTGG GACATGGGGA TAATACTACA 
CAGCTGAAAC CCAAAATGGT GAAAGTTCTT CTTGGTCATC GAGTTATTCA AGTTGCTTGT 
GGAAGTAGAG ATGCCCAGAC TCTGGCTTTG ACAGATGAAG GTTTGGTCTT TTCCTGGGGA 
GATGGTGACT TTGGGAAACT GGGCCGGGGA GGAAGTGAAG GATGTAACAT TCCTCAGAAC 
ATTGAGAGAC TTAATGGACA AGGAGTTTGC CAGATTGAGT GTGGGGCTCA ATTTTCCTTG 
GCACTTACAA AGTCAGGAGT AGTATGGACT TGGGGTAAAG GAGATTACTT TAGGCTTGGT 
CATGGCACTG ATGTTCATGT GAGAAAACCA CAAGTAGTAG AAGGACTACG AGGAAAGAAG 
ATAGTTCATG TGGCAGTAGG CGCTCTTCAC TGCTTAGCTG TCACTGATAC AGGTCAAGTG 
TATGCTTGGG GTGACAATGA TCATGGACAA CAAGGCAATG GGACTACTAC TGTCAACAGA 
AAGCCTACCC TTGTACAAGG CTTAGAAGGA CAAAAAATCA CTCGGGTAGC TTGTGGCTCA 
TCTCATAGTG TGGCGTGGAC AACAGTGGAT GTAGCAACCC CATCTGTTCA TGAACCTGTC 
CTCTTTCAAA CTGCTAGGGA CCCTTTAGGC GCTTCTTACT TAGGTGTTCC TTCCGATGCT 
GATTCTTCTG CTGCCAGTAA CAAAATCAGT GGTGTAAATA GTTCCAAGCC TAACCGCCCT 
TCCCTTGCTA AGATTCTCTT GTCTTTGGAT GGAAACCTTG CCAAACAGCA AGCATTATCT 
CATATACTTA CAGCATTGCA AATTATGTAT GCGAGAGATG CAGTAGTTGG AGCATTAATG 
CCTGCTAGTA TGATCGCACC AGTGGAATGC TCATCTTCTT CATCACCTGC ACCCCCCTCA 
GATGTGTCTT CAGCAGGAAG TCCCATGAAT GGTGAAGAGT CTGTGTTAGC TGTGGATGTA 
GAAGATAGAC TAAGCCCAAA TCCATGGCAA GACAAGAGAG GGGAGGTAGT TTCTTCAGAA 
GATGCTGTGA CTCCATCAGC AGTGACTCCC TCAGCTTCCT CTGCCTCTTC TCGACCATTT 
ATTCCAGTAA CTGATGATCC TGGAGCTGCC AGTATCATTG CAGAAACCAT GACTAAAACC 
AAAGAAGATG TTGAAAGCCA AAATAAAGTA TCAGGTCCGG AACCTCAATA TTTGGATGAA 
TTTACTAGTC TCTTAGTGCC AGATGATACC CGTGTCATGG TTGACTTGCT CAAGCTGTCT 
GTTTCTAATC GAGCTGGTGA AAAGGGAAAA GATGTTCTTT CAGCTGTGCT TTCTGGTATG 
GGCACTGCTT ATCCACAGGT AGCTGATATG TTGTTGGAGC TCTGTGTTAC AGAGTTAGAA 
GATGTTGCCA CAGACTCTCA GAGTGGTCGA CTCTCTTCCC AACCTGTTGT AGTAGAAAGT 
AGTCATCCGT ATACTGATGA TACCTCTACC AGTGGTACTG TCAAAATACC AGGTGCTGAA 
GGACTCAGAG TAGAGTTTGA TCGACAATGT TCTACAGAAC GGCGTCATGA CCCTCTCACA 
ATCATGGATG GTGTCAATAG GATAGTTTCA GTTCGATCAG GTCGAGAATG GTCTGATTGG 
TCCAGTGAAC TTCGAATTCC TGGAGATGAA TTAAAGTGGA AGTTCATCAG TGATGGATCA 
GTGAATGGAT GGGGTTGGCG ATTCACTGTC TATCCTATTA TGCCAGCTGC AGGCCCTAAA 
GATCTTCTTT CAGATCGGTG TATTCTTTCC TGTCCCTCAA TGGACTTGGT TACATGCTTG 
TTGGACTTTA GATTAAATTT AGCTTCCAAT AGAAGTATTG TTCCTCGTTT GGCAGCATCA 
CTTGCAGCAT GTGCTCAATT GAGTGCTCTA GCTGCTGGTC ACAGAATGTG GGCCCTTCAA 
AGACTGAGGA AACTGCTTAC AACTGAATTT GGACAATCAA TCAATATAAA TAGGATACTT 
GGAGATAATG ATGGAGAAAC AAGAACCATG AGTTTTACCG GTAGTGCTTT AGCAGCTTTG 
GTAAAAGGTC TTCCAGAAGC TTTGCAAAGA CAGTTTGAAT ATGAAGATCC GATTGTTAGA 
GGAGGCAAAC AACTGCTCCA TAGCCCTTTC TTTAAGGTGC TGGTAGCTCT TGCTTGTGAC 
CTAGAACTGG ATACTCTTCC TTGCTGTGCT GAGACACACA AATGGGCTTG GTTTAGAAGA 
TACTGTATGG CCTCTAGAGT TTCAGTAGCC TTGGACAAAC GAACACCATT ACCTCGCCTT 
TTCCTTGATG AGGTGGCAAA GAAAATCAGG GAGTTAATGG CCGACAGTGA AAATCTGGAT 
GTTCTGCATG AGAGCCATGA CATTTTTAAG AGAGAACAAG ATGAACAACT CGTCCAGTGG 
ATGAACAGGA GACCTGATGA CTGGACTCTT TCTGCTGGAG GCAGTGGAAC AATTTATGGT 
TGGGGTCACA ATCATCGAGG ACAGCTTGGC GGTATTGAGG GAGCAAAAGT CAAAGTTCCA 
ACACCTTGTG AAGCTCTTGC AGCTCTGCGG CCCATACAGT TAATTGGAGG GGAACAGACT 
CTCTTTGCGG TAACTGCTGA TGGGAAGCTT TATGCTACAG GGTATGGTGC AGGTGGCAGA 
TTGGGAATTG GAGGAACTGA ATCAGTTTCT ACTCCAACAT TGCTTGAATC TATTCAGCAT 
GTATTTATAA AAAAAGTTGC TGTGAACTCT GGAGGAAAAC ACTGCTTAGC ACTCTCATCA 
GAAGGAGAAG TTTATTCTTG GGGTGAGGCA GAAGATGGAA AATTGGGGCA TGGAAACAGA 
AGCCCCTGTG ATCGCCCACG TGTAATTGAA TCTTTGCGAG GAATAGAAGT TGTTGATATT 
GCTGCAGGTG GAGCCCACAG TGCATGTATT ACAGCAGCTG GTGACCTCTA TACGTGGGGT 
AAAGGAAGAT ATGGTCGCCT AGGTCATGGG GATAGTGAAG ATCAGTTAAA ACCAAAACTG 
GTGGAAGCTC TCCAAGGCTA TCGAGTTATT GATATTGCCT GTGGCAGTGG AGATGCACAG 
ACACTTTGCC TTACTGATGA TGATACTGTA TGGTCCTGGG GTGATGGGGA CTATGGAAAA 
CTTGGCAGAG GAGGCAGTGA TGGCTGTAAA GTGCCAATGA AGATTGATTC TCTCACAGGT 
CTTGGAGTGG TTAAAGTAGA GTGTGGATCA CAATTTTCTG TTGCACTTAC CAAGTCTGGA 
GCAGTTTATA CTTGGGGTAA AGGCGATTAT CACAGGTTGG GTCATGGATC AGACGACCAT 
GTTAGACGGC CTCGGCAAGT GCAAGGCCTA CAAGGAAAGA AAGTAATAGC AATTGCTACT 
GGTTCTTTAC ACTGTGTGTG CTGCACTGAA GACGGTGAAG TTTATACTTG GGGTGACAAT 
GATGAAGGGC AACTTGGGGA TGGAACAACT AATGCAATCC AGAGACCTCG GTTGGTGGCA 
GCACTTCAGG GTAAAAAGAT AAATAGGGTG GCTTGTGGTT CAGCACACAC CTTAGCGTGG 
TCCACGAGTA AACCTGCCAA TGCTGGGAAG CTTCCAACAC AGGTTCCTAT GGAATACAAC 
CATTTACAGG AATTTCTCAT AATTCCTTTA CGAAACAGAC TTTTGTTATT ACATCATATC 
TCAGAACTCT TTTGTCCATG CATTCCCATG TTTGACCTTG AGGGGCGTCT TGATGAAACT 
GGCCAAGGAC CTTCAGTGGG ATTTGACACA TTACGAGGAA TTCTGATATC TCAGGGAAAG 
GAAGCAGCTT TCCGAAAAGT TGTACAAGCA ACTATGGTGA GAGATCGTCA ACATGGTCCT 
GTAGTTGAAT TAAACAGAAT ACAGGTAAAA CGATCAAGAA GTAAGGGTGG TTTGGCTGGT 
CCTGATGGTA CTAAATCTGT TTTTGGCCAA ATGTGTGCTA AAATGATTTC ATTTAGTCCA 
GACAGTCTTC TTCTACCTCA TCGAGTATGG AAAGTCAAGT TTGTTGGCGA GTCTGTGGAT 
GATTGTGGAG GTGGTTATAG TGAATCAATA GCAGAAATGT GTGAAGAACT TCAGAATGGA 
CTTACTCCTC TCCTAATTGT AACTCCAAAT GGCAGGGATG AATCTGGAGC CAATAGAGAT 
TGCTTCTTAT TGAACCCAGC TGCCAGATCT TCTTTGCATA CCAATATGTT TCGTTTCTTG 
GGTGTATTGC TAGGCATTGC CATTCGAACT GGGAGTCCAT TAAGCCTCAA CCTTGCAGAA 
CCGGTTTGGA AGCAACTGGC AGGAATGAAC CTTACAATTG CTGATCTCAG TGAGGTTGAT 
AAGGATTTTA TTCCTGGCCT GATGTACATT CGGGACAATG AAGCTACTTC TGAGGAATTT 
GAAGCAATGA GCCTTCCCTT CACAGTGCCA AGTGCAAGTG GGCAGGATAT TCAATTGAGC 
TCTAAATATA CCCACATCAC ATTGGATAAC CGTGCTGAGT ATGTTAGATT GGCAATAAAT 
TACAGACTCC ATGAATTTGA TGAACAAGTT GCTGCAGTTC GGGAAGGAAT GGCTCGAGTT 
GTCCCTGTTC CTCTTCTTTC ACTCTTTACT GGATATGAAC TGGAAACTAT GGTGTGTGGA 
AGCCCCGATA TTCCACTTCA TCTTCTAAAA TCTGTAGCCA CATATAAAGG AATTGAACCT 
ACTGCTTCTT TGATCCAGTG GTTCTGGGAA GTGATGGAAT CTTTCTCTAA TACAGAGCGC 
TCTCTCTTTC TTCGTTTTGT GTGGGGCAGA ACACGACTTC CTAGAACAAT TGCTGATTTC 
AGAGGCAGAG ATTTTGTTAT TCAGGTTTTG GATAAGTACA ATCCACCTGA CCACTTCCTT 
CCAGAGTCAT ATACATGCTT CTTTTTGCTG AAGTTACCCA GGTATTCCTG TAAACAAGTA 
CTAGAGGAAA AACTGAAATA TGCCATTCAT TTTTGCAAGT CTATAGACAC TGATGATTAT 
GCCCGGATAG CACTCACAGG AGAACCAGCT GCTGATGACA GCAGTGATGA TTCAGATAAT 
GAAGATGCAG ATTCATTTGC ATCGGACTCT ACTCAAGATT ATTTAACAGG ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031814948.1
CDS	258..14774
Translation	MSSESFCLAAQARFDSKWLKTDLQLAFTRDGLCGLWNEMVKDGEIVYPGTDVIQNGELPPRKDDTMECQSGTKKEDQNDKEKKDEEEIPVPMYKAKSLVESWVWGKQPDVNELKECLYVLVKEQQTLATRMATTTLSAVRLKQRLVILERYFIALNRTVLQENVKVKWKSNSIPLPTVDKKSSRPIGKGVEGLARVGSRAALSFAFAFLRRAWRSGEDADLCSELLQESLDALRALPEASLFDESTVSSVWLEVVERATKFLRSVVTGDVHGTASTKGPGNIPLQDQHLALAILLELAVQRGTLSQMLSAIMLLLQLWDNGTRETDNERSAQGTSAPLLPLLQRFQSIICNKDILNTEDEIQMLSGLLSPNESFLRYLTLPQDNELAIDLRQTAVVIMAHLDRLATPCMPPQCSSPTSHKGSLQEVIGWGLIGWKYYANVIGPIQCEGLANLGITQIACAEKRFLILSRNGRVYMQAYNSDTLGPQLVQGLASRNIVKIAAHSDGHHYLALAATGEVFSWGCGDGGRLGHGDTVPLEEPKMISALSGKQAGKHVVHIACGSTYSAAITAEGELYTWGRGNYGRLGHGSSEDQTIPMLVTGLKGLKVIDVSCGSGDAQTLAVTENGQVWSWGDGDYGKLGRGGSDGCKTPKLIEKLQDLDVVKVRCGSQFSVALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVVDVAAGSTHCLALTEDSEVHSWGSNDQCQHFDTLRITKPEPTALPGLDTKHIVGIACGPAQSFAWSSCSEWSIGLRVPFVVDVCPMTFEQLDLLLRQVSEGMDGTSDWPPPQEKECMAVATLNLLRLQLHAAISHQVDPECLGLGLGSVLLNSLKQTVVTLASNAGVLNTVQSAAQAVLQSGWSVLLPTAEERARALSALLPSAVSGNDMNVSPGRRFMIDLLVGSLMADGGLESALNAAITAEIQDIEAKKEAQKEKEIDEQEANASTLHRSRTPLDKDLINTGIYESAGKQSLPLVQLIQQLLRNIASQTIAKLKDVARRILSCIEHDQYSQDRSASLDLLLRFQRLLVSKLYPGDSGGQVSNSFSPELLGVGSLLKKYTALLCTHIGDILPVATSIASSSYRHFAEVSRVVDGDLTGVLLPELIVSIVLLLNKSPGLMQEAGAIPLLAGLLEHLDRFNHLAPGKERDDNEDLAWPGIMGSFFTGQNSRNNEEVTLIRKADLENHNKDGGFWTVIDGKVYDIKDFQTQSLTGNSILAQFAGDDPVVALEAALQFEDTRDSMHAFCVGQYMEPDQEVITTPDLTSLSSPLIDPERNLGLLLGLHASYLAMSTPLSPVEIECAKWLKSSIFSGGLQTSQIHYSYNEEKDEDHCSSPGNTTPNKSKLYSHRLTLSDHSQPFLQAIADNNIQDHTVKDFLCQIERYCKQCHLTTPITFPPEHPVEEVGRLLLCCLLKHEDLGHVALSLVHPVTLGVDQVKHRTLPKSVVDVCRVVYQAKCSLIKTHQEQGRSYKEVCAPVIERLRFLFNELRPAVCNDLSIMSKLKLLSSLPRWRRIAQKMIRERRKLKRVPKKSEPTDVEESKIGNEEGDLEEACILPHSPVSVDKRPVPVKSPKDKWQPLLSTVTGVHKYKWLKQNVQGLYPQSALLSTIVEFALKEEPVDVEKMRKCLLKQLERAEVRLEGIDTILKLANKNFLLSSVQYAMFCGWQRLIPEGTNIGEPLTDCLKDVDLIPPFNRMLLEVTFGKLYGWAIQNIRNVLLDANARFKELGIQPVPLQTITNENPAGPSLGTIPQARFLLVMLNMLTLQYGANNLNLLLNSGMLALTQTTLRLIGPSSDNIEEEMNASARGASATVLEESRKETTPVQLPVSGPELAAMMKIGTRVMRGVDWKWGDQDGPPPGLGRVIGELGEDGWIRVQWDTGSTNSYRMGKEGKYDLKLAEPPPAAQPSTEDSDTEDDSESEQMERNFHPTSIMLTSTVNLLQTLCLCAGIHAEIMQNEATRTLCGLLRMLVESGTVDKTSSLPNKMVYKEQHRSWCTLGFIRSIALMPQMCSTLSTSQWITLLMKIVEGHESFTAASLQRQILAVHLLQAVLPSWDKTERSRDMKFLVEKLFGFLGSLLSTCSSDIPLLRESTLRRRKSRPQASLTATHSSTLAEEIVALLRTLHSLSQWNGLINKYINSQLNSITHIFAGKQSEGTVLEDYFPDSENPEVDGLMAVLAVIGGIDSRLRLGGQVVHDEFGEGTVTRITPKGKITVQFCDMRICRICPLSQLKPLPVMAFNVNNLPFTESMLSVWAQLVNLAGSKLEKHRIKKSTNQALTGQVDLDLLRCQQLKLYILKAGRALLSHQDKLRQILSQPALQENGVLSTDDGTASPDIGDMSPEGPQPPMILLQQLLTSATQPSPVKAIFDKQELEAAALAVCQCLAVESTHPSTPVFEECSSSEATTPVTVQHIRSVKVKKRKQSPLPALPIVVQLMEMGFPRKNIEFALKSLTGSASGLPGVEALVGWLLDHPDVQITDLSDADTVSDEYSDDEIVEDVEETEASYPVSSCAVVTESHTYKKRADFLSNDDYAVYVRENIQVGMMVRCCRTYEEVCEGDVGKVIKLDRDGLHDLNVQCDWQQKGSTYWVRYIHVELIGFPPQSSPSHIKIGDKVRVKTSVTTPKYKWGSVTHRSVGVVKAFSANGKDVIVDFPQQSHWTGLLSEMELVPSIHPGVTCDGCQMFPINGPRFKCRSCDDFDFCETCFKTKKHNTRHSFGRINEPGQSLVFCGRSGKQLKRCHSSQPGMLLDNWSRIVKNLNVSSSVNQASRLIDGSEPCWQSSGSQGKHWIRLEIFPDVLVHRLKMIVDPADSSYMPSLVVVSGGNSLNNLIELKTININPADTTVPLLNDCTEYHRYIEVAIKQCRSSGIDCKIHGLIILGRIRAEDEDLAAVPFLASDNEEEDDEKANTGSLIRKKASGLESAATIRTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALNVVQVAGGSKSLFAVTVEGKVYACGEATNGRLGLGISSGTVPIPRQITALSNYVVKKVAVHSGGRHAMALTVDGKIFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAAITSSGELYTWGLGEYGRLGHGDNTTQLKPKMVKVLLGHRVIQVACGSRDAQTLALTDEGLVFSWGDGDFGKLGRGGSEGCNIPQNIERLNGQGVCQIECGAQFSLALTKSGVVWTWGKGDYFRLGHGTDVHVRKPQVVEGLRGKKIVHVAVGALHCLAVTDTGQVYAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSVAWTTVDVATPSVHEPVLFQTARDPLGASYLGVPSDADSSAASNKISGVNSSKPNRPSLAKILLSLDGNLAKQQALSHILTALQIMYARDAVVGALMPASMIAPVECSSSSSPAPPSDVSSAGSPMNGEESVLAVDVEDRLSPNPWQDKRGEVVSSEDAVTPSAVTPSASSASSRPFIPVTDDPGAASIIAETMTKTKEDVESQNKVSGPEPQYLDEFTSLLVPDDTRVMVDLLKLSVSNRAGEKGKDVLSAVLSGMGTAYPQVADMLLELCVTELEDVATDSQSGRLSSQPVVVESSHPYTDDTSTSGTVKIPGAEGLRVEFDRQCSTERRHDPLTIMDGVNRIVSVRSGREWSDWSSELRIPGDELKWKFISDGSVNGWGWRFTVYPIMPAAGPKDLLSDRCILSCPSMDLVTCLLDFRLNLASNRSIVPRLAASLAACAQLSALAAGHRMWALQRLRKLLTTEFGQSININRILGDNDGETRTMSFTGSALAALVKGLPEALQRQFEYEDPIVRGGKQLLHSPFFKVLVALACDLELDTLPCCAETHKWAWFRRYCMASRVSVALDKRTPLPRLFLDEVAKKIRELMADSENLDVLHESHDIFKREQDEQLVQWMNRRPDDWTLSAGGSGTIYGWGHNHRGQLGGIEGAKVKVPTPCEALAALRPIQLIGGEQTLFAVTADGKLYATGYGAGGRLGIGGTESVSTPTLLESIQHVFIKKVAVNSGGKHCLALSSEGEVYSWGEAEDGKLGHGNRSPCDRPRVIESLRGIEVVDIAAGGAHSACITAAGDLYTWGKGRYGRLGHGDSEDQLKPKLVEALQGYRVIDIACGSGDAQTLCLTDDDTVWSWGDGDYGKLGRGGSDGCKVPMKIDSLTGLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLGHGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCTEDGEVYTWGDNDEGQLGDGTTNAIQRPRLVAALQGKKINRVACGSAHTLAWSTSKPANAGKLPTQVPMEYNHLQEFLIIPLRNRLLLLHHISELFCPCIPMFDLEGRLDETGQGPSVGFDTLRGILISQGKEAAFRKVVQATMVRDRQHGPVVELNRIQVKRSRSKGGLAGPDGTKSVFGQMCAKMISFSPDSLLLPHRVWKVKFVGESVDDCGGGYSESIAEMCEELQNGLTPLLIVTPNGRDESGANRDCFLLNPAARSSLHTNMFRFLGVLLGIAIRTGSPLSLNLAEPVWKQLAGMNLTIADLSEVDKDFIPGLMYIRDNEATSEEFEAMSLPFTVPSASGQDIQLSSKYTHITLDNRAEYVRLAINYRLHEFDEQVAAVREGMARVVPVPLLSLFTGYELETMVCGSPDIPLHLLKSVATYKGIEPTASLIQWFWEVMESFSNTERSLFLRFVWGRTRLPRTIADFRGRDFVIQVLDKYNPPDHFLPESYTCFFLLKLPRYSCKQVLEEKLKYAIHFCKSIDTDDYARIALTGEPAADDSSDDSDNEDADSFASDSTQDYLTGH

Target ORF information:

RefSeq Version	XM_031959088.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HECT and RLD domain containing E3 ubiquitin protein ligase 2 (HERC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031959088.1

ORF Insert Sequence:

ATGTCTTCTG AATCCTTCTG TTTAGCTGCC CAGGCTAGAT TTGACTCCAA GTGGTTAAAA 
ACAGATTTAC AGCTTGCTTT TACAAGAGAT GGACTCTGTG GCCTATGGAA TGAAATGGTC 
AAAGATGGAG AAATTGTATA TCCTGGGACA GATGTAATAC AGAATGGAGA ACTACCTCCT 
CGAAAAGATG ACACCATGGA ATGTCAGAGT GGAACAAAGA AAGAAGATCA AAATGATAAA 
GAGAAGAAAG ATGAAGAGGA AATTCCAGTC CCTATGTATA AGGCCAAATC ACTTGTGGAG 
AGTTGGGTAT GGGGCAAACA ACCAGATGTG AACGAGTTGA AAGAGTGCCT TTATGTGCTA 
GTGAAAGAAC AGCAAACTCT TGCTACACGG ATGGCTACAA CAACTCTTTC AGCAGTAAGG 
CTAAAGCAGA GGCTGGTTAT ACTGGAACGA TATTTCATTG CACTGAATAG AACTGTTCTT 
CAGGAAAATG TCAAAGTTAA GTGGAAAAGC AACAGTATTC CTTTACCTAC TGTCGACAAA 
AAAAGTTCTA GACCTATAGG AAAAGGTGTA GAAGGACTTG CAAGAGTTGG ATCCAGAGCA 
GCCCTTTCAT TTGCTTTTGC ATTCCTACGC AGAGCTTGGC GGTCAGGGGA AGATGCCGAT 
CTATGCAGTG AATTGTTACA GGAGTCTCTT GATGCCTTGA GAGCCCTACC AGAAGCATCT 
CTTTTTGATG AAAGCACAGT GTCATCTGTT TGGTTGGAAG TTGTGGAGAG AGCAACTAAA 
TTCCTAAGGT CTGTTGTTAC TGGTGATGTT CATGGAACTG CAAGTACCAA AGGACCAGGA 
AACATTCCAT TACAAGACCA GCACTTAGCA CTTGCCATAT TGTTAGAGTT GGCTGTGCAG 
AGAGGCACTC TAAGCCAAAT GTTATCTGCC ATTATGTTAC TACTTCAATT ATGGGACAAT 
GGGACAAGGG AAACAGATAA TGAACGATCT GCCCAAGGAA CTAGTGCACC ACTTCTCCCT 
TTATTACAAA GATTTCAGAG TATAATCTGC AATAAAGACA TACTTAATAC TGAAGATGAG 
ATTCAGATGC TGTCTGGTCT TCTGAGTCCC AATGAAAGTT TTTTGAGGTA CCTTACTTTA 
CCACAAGACA ATGAATTAGC CATTGATTTG CGTCAAACTG CAGTAGTGAT TATGGCGCAT 
TTAGATCGTC TGGCCACTCC CTGTATGCCT CCTCAGTGTA GTTCTCCAAC ATCTCATAAG 
GGATCTTTAC AAGAAGTCAT AGGTTGGGGC TTAATAGGAT GGAAATACTA TGCTAATGTG 
ATTGGCCCAA TTCAATGTGA AGGACTTGCA AATCTTGGGA TCACACAGAT TGCCTGTGCA 
GAAAAGCGTT TCCTGATTTT GTCTCGGAAT GGGCGAGTTT ACATGCAGGC TTACAACAGT 
GACACATTGG GACCCCAACT AGTTCAAGGT CTTGCCTCTA GAAATATTGT GAAAATTGCA 
GCACATTCAG ATGGTCATCA TTATCTGGCT TTGGCAGCCA CTGGAGAGGT CTTTTCTTGG 
GGCTGTGGAG ATGGTGGACG GTTAGGCCAT GGTGATACAG TGCCTCTAGA AGAACCTAAG 
ATGATATCTG CTCTCTCAGG AAAGCAAGCT GGGAAACATG TAGTTCATAT CGCATGTGGA 
AGTACTTATA GTGCAGCTAT TACAGCTGAA GGAGAGTTAT ATACTTGGGG TCGAGGAAAC 
TATGGCAGGC TGGGACATGG CTCAAGTGAA GATCAGACAA TTCCAATGCT AGTAACTGGA 
CTTAAAGGAC TGAAGGTTAT TGATGTCTCT TGTGGGAGTG GGGATGCTCA GACTCTTGCA 
GTGACTGAGA ATGGTCAAGT CTGGTCTTGG GGCGATGGTG ATTATGGAAA ACTAGGAAGA 
GGTGGTAGTG ATGGTTGTAA AACACCAAAA CTGATTGAAA AACTTCAAGA CTTAGATGTT 
GTTAAAGTAC GTTGTGGAAG CCAATTTTCC GTTGCTTTAA CAAAAGATGG TCAAGTTTAC 
TCGTGGGGAA AAGGTGACAA TCAGAGACTT GGGCATGGAA CAGAGGAGCA TGTTCGATAT 
CCGAAACTGT TAGAAGGCTT ACAAGGTAAA AAAGTCGTTG ATGTGGCTGC TGGTTCTACA 
CACTGCTTAG CACTTACTGA GGATAGCGAA GTTCATAGCT GGGGGAGTAA TGATCAGTGT 
CAGCACTTTG ACACCCTTCG GATTACCAAA CCTGAACCTA CTGCATTGCC AGGATTAGAT 
ACAAAACATA TAGTTGGAAT TGCTTGTGGG CCCGCTCAGA GTTTTGCTTG GTCTTCTTGT 
TCTGAGTGGT CCATTGGCCT ACGTGTACCT TTTGTGGTGG ATGTTTGTCC CATGACATTT 
GAACAGTTGG ATCTCTTACT ACGACAAGTA AGTGAGGGAA TGGATGGCAC CTCTGACTGG 
CCACCACCAC AGGAGAAAGA ATGTATGGCA GTGGCAACAT TGAACCTTCT GAGACTTCAG 
TTGCATGCTG CTATTAGTCA CCAGGTTGAT CCGGAATGTC TTGGATTGGG TCTGGGAAGT 
GTACTCTTGA ATAGCTTGAA GCAGACAGTT GTAACACTAG CAAGTAATGC TGGAGTTCTT 
AATACAGTGC AATCAGCTGC TCAAGCAGTA CTGCAGAGTG GATGGTCTGT GCTGCTTCCA 
ACTGCAGAGG AACGAGCAAG GGCTCTTTCA GCTCTCTTAC CCAGTGCAGT TTCAGGAAAT 
GACATGAATG TAAGTCCAGG ACGTCGTTTT ATGATTGATC TTCTTGTAGG CAGTTTGATG 
GCTGATGGAG GATTAGAATC AGCATTAAAT GCTGCCATCA CTGCAGAAAT CCAGGATATT 
GAAGCAAAAA AAGAAGCTCA AAAGGAAAAA GAGATTGATG AGCAGGAAGC AAATGCTTCA 
ACACTTCATC GGAGTAGGAC ACCATTAGAT AAAGACCTCA TTAATACTGG AATTTATGAA 
TCTGCCGGAA AACAGAGTTT GCCTCTGGTT CAATTAATAC AACAGCTTCT TAGAAATATT 
GCTTCCCAGA CAATAGCCAA ATTGAAAGAT GTTGCTCGTC GAATTTTGTC CTGTATAGAA 
CATGATCAAT ATAGTCAAGA CAGATCTGCC TCCCTGGATT TGTTGCTTCG TTTTCAGCGT 
CTGCTGGTCA GTAAACTCTA TCCAGGAGAC AGTGGTGGTC AAGTTTCAAA TTCCTTTAGT 
CCTGAGCTAT TAGGAGTTGG TTCTTTGCTG AAAAAATATA CAGCTCTTTT GTGCACACAC 
ATTGGAGATA TATTGCCTGT GGCAACTAGT ATTGCATCTT CCAGTTATCG GCATTTTGCA 
GAAGTATCTC GTGTTGTGGA TGGTGATCTA ACAGGTGTTC TTCTTCCAGA GTTGATAGTA 
TCTATTGTCC TTTTGCTCAA TAAAAGTCCT GGATTAATGC AGGAAGCTGG GGCAATTCCT 
CTCTTAGCTG GTCTACTGGA ACATCTAGAT AGATTTAACC ATTTGGCACC AGGAAAAGAG 
AGAGATGATA ATGAGGATTT AGCCTGGCCT GGAATAATGG GATCATTTTT TACAGGTCAG 
AATAGTAGAA ATAATGAGGA AGTGACGCTC ATACGTAAAG CTGATCTGGA GAATCACAAT 
AAAGATGGAG GCTTCTGGAC TGTGATTGAC GGGAAAGTGT ATGATATAAA AGACTTTCAG 
ACACAGTCTT TAACAGGCAA TAGCATTCTT GCTCAGTTTG CAGGTGATGA CCCGGTGGTT 
GCTTTGGAAG CTGCTTTACA GTTTGAGGAC ACAAGGGATT CAATGCATGC ATTTTGTGTT 
GGCCAGTATA TGGAGCCTGA CCAGGAAGTG ATTACAACGC CAGACCTTAC CAGTCTCTCT 
TCACCTCTCA TAGATCCAGA AAGAAATCTG GGTCTCCTCC TTGGACTTCA TGCTTCCTAT 
TTAGCAATGA GCACACCACT ATCTCCTGTG GAGATTGAAT GTGCCAAATG GCTTAAGTCA 
TCCATATTCT CTGGAGGATT GCAAACCAGT CAGATACATT ATAGTTACAA TGAAGAGAAA 
GATGAAGACC ACTGCAGTTC TCCTGGAAAT ACTACTCCAA ACAAATCCAA ACTCTATTCC 
CATAGATTGA CTTTGAGTGA TCATTCCCAA CCATTTTTGC AAGCCATTGC AGATAATAAC 
ATTCAGGATC ATACCGTGAA GGATTTTTTA TGTCAGATAG AAAGGTATTG CAAGCAATGC 
CATTTGACCA CACCGATAAC ATTTCCTCCT GAACATCCTG TGGAAGAAGT GGGACGTTTA 
CTCTTATGTT GTCTTCTAAA GCATGAAGAT TTAGGTCATG TGGCCTTATC TTTAGTTCAC 
CCAGTTACAC TTGGTGTTGA CCAAGTCAAA CACAGAACGT TGCCTAAGTC TGTAGTGGAT 
GTTTGTAGAG TCGTCTACCA GGCAAAATGC TCACTAATAA AGACACATCA GGAACAGGGA 
CGTTCTTATA AGGAAGTGTG TGCTCCTGTT ATTGAACGTT TGCGATTTCT TTTTAATGAA 
TTGCGTCCTG CAGTCTGCAA TGACCTCTCA ATAATGTCCA AACTTAAACT TTTAAGCTCT 
TTGCCTCGTT GGAGAAGAAT AGCTCAGAAG ATGATACGAG AGAGAAGGAA ATTAAAAAGA 
GTACCCAAAA AGTCAGAACC TACTGATGTG GAAGAATCTA AGATTGGCAA TGAAGAGGGT 
GACTTAGAAG AAGCCTGTAT TCTTCCTCAT AGTCCTGTAT CTGTAGATAA AAGACCTGTT 
CCAGTTAAAT CACCTAAGGA TAAATGGCAG CCACTTCTGA GTACAGTTAC AGGTGTTCAC 
AAATACAAAT GGCTTAAACA GAATGTTCAA GGCCTTTATC CTCAGTCTGC ACTGCTTAGC 
ACAATTGTTG AATTTGCGCT TAAAGAAGAG CCAGTTGATG TGGAAAAGAT GAGAAAGTGC 
TTACTCAAAC AGTTGGAGAG AGCAGAAGTT CGTTTGGAAG GAATAGACAC GATTTTAAAA 
TTGGCAAATA AAAATTTCTT GCTTTCGTCT GTGCAATATG CTATGTTCTG TGGATGGCAA 
AGACTTATCC CAGAAGGAAC CAATATAGGG GAACCACTTA CAGATTGTTT AAAGGATGTT 
GATCTGATTC CACCATTTAA TCGAATGCTA CTAGAAGTAA CTTTTGGAAA GTTGTATGGT 
TGGGCCATTC AGAATATTCG AAATGTTTTG TTGGATGCAA ATGCCAGATT TAAAGAGCTA 
GGTATCCAGC CTGTTCCTCT GCAAACTATT ACTAATGAGA ATCCAGCAGG GCCAAGCCTT 
GGAACCATCC CACAAGCACG TTTTCTCCTG GTTATGCTCA ACATGCTTAC CCTTCAATAT 
GGTGCAAACA ACTTGAATCT TCTTTTGAAC TCTGGCATGC TAGCATTGAC TCAGACAACA 
TTGCGATTAA TAGGTCCTAG TTCTGATAAT ATCGAGGAAG AAATGAATGC CTCTGCACGA 
GGAGCTTCAG CTACTGTTTT GGAAGAATCC AGAAAAGAAA CCACTCCAGT ACAACTTCCT 
GTTTCAGGGC CAGAACTGGC AGCAATGATG AAAATTGGTA CCAGGGTCAT GAGGGGAGTG 
GACTGGAAAT GGGGAGATCA GGATGGTCCA CCTCCAGGTT TAGGTCGTGT GATTGGTGAA 
TTGGGAGAGG ATGGATGGAT AAGGGTTCAG TGGGATACAG GCAGTACTAA TTCATACCGA 
ATGGGAAAGG AAGGAAAGTA TGATCTTAAA TTGGCTGAGC CTCCACCTGC AGCTCAGCCC 
TCAACAGAGG ATTCGGACAC TGAGGATGAT TCTGAAAGTG AACAAATGGA AAGAAACTTC 
CACCCTACTT CTATTATGCT TACAAGTACT GTTAATTTAC TCCAAACTCT CTGTCTCTGT 
GCTGGAATTC ATGCAGAAAT CATGCAAAAT GAAGCTACAA GAACTCTGTG TGGTCTACTT 
AGAATGTTAG TGGAAAGTGG AACAGTAGAT AAAACATCTT CTTTACCAAA TAAGATGGTT 
TATAAAGAGC AGCACAGAAG TTGGTGTACA CTTGGATTTA TTCGAAGTAT AGCACTTATG 
CCTCAAATGT GTAGCACCCT TAGCACATCT CAGTGGATCA CATTACTAAT GAAAATTGTG 
GAAGGACACG AGTCATTTAC AGCAGCTTCG CTTCAGAGAC AGATCTTAGC AGTACATTTA 
CTACAAGCTG TACTTCCATC TTGGGACAAA ACAGAAAGAT CAAGAGATAT GAAGTTTCTT 
GTGGAAAAAC TTTTTGGCTT CTTGGGCAGT CTACTTAGTA CATGCTCTTC AGACATACCT 
TTGCTCAGAG AATCAACTCT GAGAAGGAGG AAGTCCAGAC CCCAGGCATC TTTGACTGCA 
ACCCATAGTA GTACTTTGGC AGAGGAGATA GTGGCCCTGC TAAGGACTCT ACATTCACTG 
AGCCAGTGGA ATGGACTCAT AAACAAGTAC ATCAACTCTC AGCTAAACTC CATCACCCAC 
ATCTTTGCAG GAAAACAGTC TGAAGGGACT GTGTTAGAAG ATTACTTTCC TGATTCTGAG 
AACCCTGAAG TTGATGGCCT AATGGCAGTG CTAGCTGTAA TTGGTGGAAT AGATAGTCGA 
TTGAGGCTGG GTGGCCAAGT GGTACATGAT GAATTTGGAG AAGGCACAGT GACACGCATC 
ACTCCTAAAG GCAAAATCAC AGTCCAGTTC TGTGATATGC GAATATGTAG AATTTGTCCA 
TTGAGTCAGC TAAAACCGCT TCCAGTTATG GCATTTAATG TGAACAACTT ACCATTCACG 
GAATCTATGC TCTCTGTCTG GGCTCAATTG GTCAACTTGG CAGGGAGCAA ATTAGAAAAA 
CACAGAATTA AGAAATCTAC TAATCAAGCC CTCACAGGAC AAGTAGACTT GGATTTGCTA 
AGATGCCAAC AGTTGAAATT ATACATATTG AAAGCAGGTC GAGCATTGCT TTCCCATCAG 
GATAAACTGA GGCAGATCTT ATCTCAACCA GCTTTACAAG AGAATGGAGT TCTCTCTACA 
GATGATGGTA CAGCATCCCC TGATATTGGA GATATGTCTC CTGAAGGACC TCAACCTCCA 
ATGATCCTTT TACAGCAATT GTTGACATCT GCCACACAGC CATCACCTGT AAAAGCAATA 
TTTGACAAAC AGGAACTTGA GGCTGCTGCT TTGGCTGTAT GCCAGTGTCT AGCTGTGGAG 
TCAACTCATC CATCAACTCC AGTGTTTGAA GAATGTAGCT CTAGTGAGGC CACAACACCT 
GTCACAGTCC AGCATATTCG ATCTGTAAAA GTGAAAAAGC GAAAGCAGTC TCCTCTCCCT 
GCTTTGCCTA TTGTAGTACA GCTCATGGAA ATGGGATTTC CGAGGAAAAA CATTGAATTT 
GCATTGAAGT CTCTTACTGG GAGTGCTTCA GGGTTACCAG GTGTTGAAGC CTTGGTTGGT 
TGGTTGCTGG ATCATCCTGA TGTCCAAATT ACTGATCTCT CAGATGCTGA TACAGTCTCT 
GATGAATACT CTGATGATGA AATAGTGGAA GATGTGGAAG AGACTGAAGC ATCTTACCCT 
GTGTCTAGTT GTGCTGTTGT AACGGAGAGT CACACTTATA AAAAACGAGC TGATTTCTTA 
AGTAATGATG ACTATGCAGT ATATGTTAGA GAAAACATTC AGGTAGGAAT GATGGTTAGG 
TGCTGCAGAA CGTATGAAGA AGTTTGTGAA GGAGATGTAG GTAAAGTGAT TAAATTGGAC 
AGAGATGGAT TACACGACCT CAATGTGCAG TGTGATTGGC AACAAAAGGG GAGTACCTAT 
TGGGTTAGGT ATATCCATGT TGAACTTATA GGTTTCCCAC CACAGAGTTC TCCTTCCCAT 
ATCAAGATTG GTGATAAAGT TAGGGTGAAA ACATCAGTAA CCACACCAAA ATATAAATGG 
GGATCAGTTA CTCATAGAAG TGTGGGAGTT GTGAAAGCTT TTAGTGCCAA TGGAAAAGAT 
GTTATTGTGG ATTTTCCTCA GCAGTCTCAT TGGACAGGAT TATTGTCAGA AATGGAATTG 
GTGCCTAGTA TTCATCCTGG AGTTACGTGT GATGGCTGCC AAATGTTTCC TATCAATGGT 
CCTCGTTTCA AATGCAGGAG TTGTGATGAT TTTGACTTTT GTGAGACCTG TTTTAAAACC 
AAGAAGCATA ATACCAGACA TAGTTTTGGC AGAATAAATG AACCAGGTCA GTCTCTTGTA 
TTTTGTGGGC GTTCTGGGAA ACAGCTAAAA CGTTGCCACA GTAGTCAGCC TGGAATGCTC 
TTGGATAATT GGTCAAGAAT TGTTAAGAAT TTAAATGTGT CCTCATCAGT AAATCAGGCT 
TCTCGCTTGA TTGATGGCAG TGAACCATGT TGGCAGTCAT CTGGATCACA AGGAAAGCAT 
TGGATTCGAC TGGAGATTTT CCCCGATGTT CTTGTTCATA GACTGAAAAT GATAGTAGAT 
CCTGCTGACA GTAGCTACAT GCCTTCACTA GTTGTAGTTT CAGGTGGGAA TTCCCTAAAT 
AATTTAATTG AACTGAAGAC AATCAATATT AACCCAGCTG ATACCACAGT GCCTCTTCTA 
AATGACTGCA CAGAGTATCA CAGGTATATT GAGGTTGCCA TAAAGCAATG CAGAAGCTCA 
GGAATAGATT GTAAAATTCA TGGTCTTATT ATACTGGGAC GAATTCGGGC AGAAGATGAA 
GATTTGGCTG CTGTTCCTTT CTTAGCGTCA GATAATGAAG AAGAAGATGA TGAAAAAGCT 
AACACTGGGA GTCTTATTAG GAAGAAGGCT TCTGGATTGG AATCAGCAGC CACAATAAGG 
ACCAAAGTTT TTGTCTGGGG GCTGAATGAC AAGGATCAAT TAGGAGGATT AAAGGGTTCT 
AAGATAAAAG TTCCTTCATT CTCTGAAACC CTTTCTGCTT TGAATGTAGT TCAAGTGGCA 
GGTGGATCTA AAAGTTTGTT TGCAGTGACT GTGGAGGGTA AAGTGTATGC TTGTGGAGAA 
GCTACTAATG GACGACTGGG TTTAGGTATT TCTAGTGGAA CTGTACCCAT TCCGCGGCAG 
ATTACAGCTC TCAGCAATTA TGTGGTAAAG AAAGTTGCTG TTCACTCAGG TGGCCGGCAT 
GCTATGGCTT TAACTGTTGA TGGAAAGATA TTTTCATGGG GTGAAGGTGA TGATGGAAAA 
CTAGGACACT TCAGCAGAAT GAACTGTGAT AAACCAAGAC TGATAGAGGC ATTGAAAACC 
AAACGTATTC GTGATATAGC TTGTGGTAGT TCTCACAGTG CTGCCATTAC ATCAAGTGGT 
GAATTGTATA CATGGGGCCT TGGAGAATAT GGCCGGTTGG GACATGGGGA TAATACTACA 
CAGCTGAAAC CCAAAATGGT GAAAGTTCTT CTTGGTCATC GAGTTATTCA AGTTGCTTGT 
GGAAGTAGAG ATGCCCAGAC TCTGGCTTTG ACAGATGAAG GTTTGGTCTT TTCCTGGGGA 
GATGGTGACT TTGGGAAACT GGGCCGGGGA GGAAGTGAAG GATGTAACAT TCCTCAGAAC 
ATTGAGAGAC TTAATGGACA AGGAGTTTGC CAGATTGAGT GTGGGGCTCA ATTTTCCTTG 
GCACTTACAA AGTCAGGAGT AGTATGGACT TGGGGTAAAG GAGATTACTT TAGGCTTGGT 
CATGGCACTG ATGTTCATGT GAGAAAACCA CAAGTAGTAG AAGGACTACG AGGAAAGAAG 
ATAGTTCATG TGGCAGTAGG CGCTCTTCAC TGCTTAGCTG TCACTGATAC AGGTCAAGTG 
TATGCTTGGG GTGACAATGA TCATGGACAA CAAGGCAATG GGACTACTAC TGTCAACAGA 
AAGCCTACCC TTGTACAAGG CTTAGAAGGA CAAAAAATCA CTCGGGTAGC TTGTGGCTCA 
TCTCATAGTG TGGCGTGGAC AACAGTGGAT GTAGCAACCC CATCTGTTCA TGAACCTGTC 
CTCTTTCAAA CTGCTAGGGA CCCTTTAGGC GCTTCTTACT TAGGTGTTCC TTCCGATGCT 
GATTCTTCTG CTGCCAGTAA CAAAATCAGT GGTGTAAATA GTTCCAAGCC TAACCGCCCT 
TCCCTTGCTA AGATTCTCTT GTCTTTGGAT GGAAACCTTG CCAAACAGCA AGCATTATCT 
CATATACTTA CAGCATTGCA AATTATGTAT GCGAGAGATG CAGTAGTTGG AGCATTAATG 
CCTGCTAGTA TGATCGCACC AGTGGAATGC TCATCTTCTT CATCACCTGC ACCCCCCTCA 
GATGTGTCTT CAGCAGGAAG TCCCATGAAT GGTGAAGAGT CTGTGTTAGC TGTGGATGTA 
GAAGATAGAC TAAGCCCAAA TCCATGGCAA GACAAGAGAG GGGAGGTAGT TTCTTCAGAA 
GATGCTGTGA CTCCATCAGC AGTGACTCCC TCAGCTTCCT CTGCCTCTTC TCGACCATTT 
ATTCCAGTAA CTGATGATCC TGGAGCTGCC AGTATCATTG CAGAAACCAT GACTAAAACC 
AAAGAAGATG TTGAAAGCCA AAATAAAGTA TCAGGTCCGG AACCTCAATA TTTGGATGAA 
TTTACTAGTC TCTTAGTGCC AGATGATACC CGTGTCATGG TTGACTTGCT CAAGCTGTCT 
GTTTCTAATC GAGCTGGTGA AAAGGGAAAA GATGTTCTTT CAGCTGTGCT TTCTGGTATG 
GGCACTGCTT ATCCACAGGT AGCTGATATG TTGTTGGAGC TCTGTGTTAC AGAGTTAGAA 
GATGTTGCCA CAGACTCTCA GAGTGGTCGA CTCTCTTCCC AACCTGTTGT AGTAGAAAGT 
AGTCATCCGT ATACTGATGA TACCTCTACC AGTGGTACTG TCAAAATACC AGGTGCTGAA 
GGACTCAGAG TAGAGTTTGA TCGACAATGT TCTACAGAAC GGCGTCATGA CCCTCTCACA 
ATCATGGATG GTGTCAATAG GATAGTTTCA GTTCGATCAG GTCGAGAATG GTCTGATTGG 
TCCAGTGAAC TTCGAATTCC TGGAGATGAA TTAAAGTGGA AGTTCATCAG TGATGGATCA 
GTGAATGGAT GGGGTTGGCG ATTCACTGTC TATCCTATTA TGCCAGCTGC AGGCCCTAAA 
GATCTTCTTT CAGATCGGTG TATTCTTTCC TGTCCCTCAA TGGACTTGGT TACATGCTTG 
TTGGACTTTA GATTAAATTT AGCTTCCAAT AGAAGTATTG TTCCTCGTTT GGCAGCATCA 
CTTGCAGCAT GTGCTCAATT GAGTGCTCTA GCTGCTGGTC ACAGAATGTG GGCCCTTCAA 
AGACTGAGGA AACTGCTTAC AACTGAATTT GGACAATCAA TCAATATAAA TAGGATACTT 
GGAGATAATG ATGGAGAAAC AAGAACCATG AGTTTTACCG GTAGTGCTTT AGCAGCTTTG 
GTAAAAGGTC TTCCAGAAGC TTTGCAAAGA CAGTTTGAAT ATGAAGATCC GATTGTTAGA 
GGAGGCAAAC AACTGCTCCA TAGCCCTTTC TTTAAGGTGC TGGTAGCTCT TGCTTGTGAC 
CTAGAACTGG ATACTCTTCC TTGCTGTGCT GAGACACACA AATGGGCTTG GTTTAGAAGA 
TACTGTATGG CCTCTAGAGT TTCAGTAGCC TTGGACAAAC GAACACCATT ACCTCGCCTT 
TTCCTTGATG AGGTGGCAAA GAAAATCAGG GAGTTAATGG CCGACAGTGA AAATCTGGAT 
GTTCTGCATG AGAGCCATGA CATTTTTAAG AGAGAACAAG ATGAACAACT CGTCCAGTGG 
ATGAACAGGA GACCTGATGA CTGGACTCTT TCTGCTGGAG GCAGTGGAAC AATTTATGGT 
TGGGGTCACA ATCATCGAGG ACAGCTTGGC GGTATTGAGG GAGCAAAAGT CAAAGTTCCA 
ACACCTTGTG AAGCTCTTGC AGCTCTGCGG CCCATACAGT TAATTGGAGG GGAACAGACT 
CTCTTTGCGG TAACTGCTGA TGGGAAGCTT TATGCTACAG GGTATGGTGC AGGTGGCAGA 
TTGGGAATTG GAGGAACTGA ATCAGTTTCT ACTCCAACAT TGCTTGAATC TATTCAGCAT 
GTATTTATAA AAAAAGTTGC TGTGAACTCT GGAGGAAAAC ACTGCTTAGC ACTCTCATCA 
GAAGGAGAAG TTTATTCTTG GGGTGAGGCA GAAGATGGAA AATTGGGGCA TGGAAACAGA 
AGCCCCTGTG ATCGCCCACG TGTAATTGAA TCTTTGCGAG GAATAGAAGT TGTTGATATT 
GCTGCAGGTG GAGCCCACAG TGCATGTATT ACAGCAGCTG GTGACCTCTA TACGTGGGGT 
AAAGGAAGAT ATGGTCGCCT AGGTCATGGG GATAGTGAAG ATCAGTTAAA ACCAAAACTG 
GTGGAAGCTC TCCAAGGCTA TCGAGTTATT GATATTGCCT GTGGCAGTGG AGATGCACAG 
ACACTTTGCC TTACTGATGA TGATACTGTA TGGTCCTGGG GTGATGGGGA CTATGGAAAA 
CTTGGCAGAG GAGGCAGTGA TGGCTGTAAA GTGCCAATGA AGATTGATTC TCTCACAGGT 
CTTGGAGTGG TTAAAGTAGA GTGTGGATCA CAATTTTCTG TTGCACTTAC CAAGTCTGGA 
GCAGTTTATA CTTGGGGTAA AGGCGATTAT CACAGGTTGG GTCATGGATC AGACGACCAT 
GTTAGACGGC CTCGGCAAGT GCAAGGCCTA CAAGGAAAGA AAGTAATAGC AATTGCTACT 
GGTTCTTTAC ACTGTGTGTG CTGCACTGAA GACGGTGAAG TTTATACTTG GGGTGACAAT 
GATGAAGGGC AACTTGGGGA TGGAACAACT AATGCAATCC AGAGACCTCG GTTGGTGGCA 
GCACTTCAGG GTAAAAAGAT AAATAGGGTG GCTTGTGGTT CAGCACACAC CTTAGCGTGG 
TCCACGAGTA AACCTGCCAA TGCTGGGAAG CTTCCAACAC AGGTTCCTAT GGAATACAAC 
CATTTACAGG AATTTCTCAT AATTCCTTTA CGAAACAGAC TTTTGTTATT ACATCATATC 
TCAGAACTCT TTTGTCCATG CATTCCCATG TTTGACCTTG AGGGGCGTCT TGATGAAACT 
GGCCAAGGAC CTTCAGTGGG ATTTGACACA TTACGAGGAA TTCTGATATC TCAGGGAAAG 
GAAGCAGCTT TCCGAAAAGT TGTACAAGCA ACTATGGTGA GAGATCGTCA ACATGGTCCT 
GTAGTTGAAT TAAACAGAAT ACAGGTAAAA CGATCAAGAA GTAAGGGTGG TTTGGCTGGT 
CCTGATGGTA CTAAATCTGT TTTTGGCCAA ATGTGTGCTA AAATGATTTC ATTTAGTCCA 
GACAGTCTTC TTCTACCTCA TCGAGTATGG AAAGTCAAGT TTGTTGGCGA GTCTGTGGAT 
GATTGTGGAG GTGGTTATAG TGAATCAATA GCAGAAATGT GTGAAGAACT TCAGAATGGA 
CTTACTCCTC TCCTAATTGT AACTCCAAAT GGCAGGGATG AATCTGGAGC CAATAGAGAT 
TGCTTCTTAT TGAACCCAGC TGCCAGATCT TCTTTGCATA CCAATATGTT TCGTTTCTTG 
GGTGTATTGC TAGGCATTGC CATTCGAACT GGGAGTCCAT TAAGCCTCAA CCTTGCAGAA 
CCGGTTTGGA AGCAACTGGC AGGAATGAAC CTTACAATTG CTGATCTCAG TGAGGTTGAT 
AAGGATTTTA TTCCTGGCCT GATGTACATT CGGGACAATG AAGCTACTTC TGAGGAATTT 
GAAGCAATGA GCCTTCCCTT CACAGTGCCA AGTGCAAGTG GGCAGGATAT TCAATTGAGC 
TCTAAATATA CCCACATCAC ATTGGATAAC CGTGCTGAGT ATGTTAGATT GGCAATAAAT 
TACAGACTCC ATGAATTTGA TGAACAAGTT GCTGCAGTTC GGGAAGGAAT GGCTCGAGTT 
GTCCCTGTTC CTCTTCTTTC ACTCTTTACT GGATATGAAC TGGAAACTAT GGTGTGTGGA 
AGCCCCGATA TTCCACTTCA TCTTCTAAAA TCTGTAGCCA CATATAAAGG AATTGAACCT 
ACTGCTTCTT TGATCCAGTG GTTCTGGGAA GTGATGGAAT CTTTCTCTAA TACAGAGCGC 
TCTCTCTTTC TTCGTTTTGT GTGGGGCAGA ACACGACTTC CTAGAACAAT TGCTGATTTC 
AGAGGCAGAG ATTTTGTTAT TCAGGTTTTG GATAAGTACA ATCCACCTGA CCACTTCCTT 
CCAGAGTCAT ATACATGCTT CTTTTTGCTG AAGTTACCCA GGTATTCCTG TAAACAAGTA 
CTAGAGGAAA AACTGAAATA TGCCATTCAT TTTTGCAAGT CTATAGACAC TGATGATTAT 
GCCCGGATAG CACTCACAGG AGAACCAGCT GCTGATGACA GCAGTGATGA TTCAGATAAT 
GAAGATGCAG ATTCATTTGC ATCGGACTCT ACTCAAGATT ATTTAACAGG ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC2 ( XM_023500395.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023356163.1 Sarcophilus harrisii HECT and RLD domain containing E3 ubiquitin protein ligase 2 (HERC2), mRNA.

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CloneID	OSa152727
Clone ID Related Accession (Same CDS sequence)	XM_031959088.1 , XM_023500395.1
Accession Version	XM_023500395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 14517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	E3 ubiquitin-protein ligase HERC2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003831837.1) and transcript sequences (GEDN01122453.1, GEDN01122455.1 and GEDN01122460.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1195 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCTTCTG AATCCTTCTG TTTAGCTGCC CAGGCTAGAT TTGACTCCAA GTGGTTAAAA 
ACAGATTTAC AGCTTGCTTT TACAAGAGAT GGACTCTGTG GCCTATGGAA TGAAATGGTC 
AAAGATGGAG AAATTGTATA TCCTGGGACA GATGTAATAC AGAATGGAGA ACTACCTCCT 
CGAAAAGATG ACACCATGGA ATGTCAGAGT GGAACAAAGA AAGAAGATCA AAATGATAAA 
GAGAAGAAAG ATGAAGAGGA AATTCCAGTC CCTATGTATA AGGCCAAATC ACTTGTGGAG 
AGTTGGGTAT GGGGCAAACA ACCAGATGTG AACGAGTTGA AAGAGTGCCT TTATGTGCTA 
GTGAAAGAAC AGCAAACTCT TGCTACACGG ATGGCTACAA CAACTCTTTC AGCAGTAAGG 
CTAAAGCAGA GGCTGGTTAT ACTGGAACGA TATTTCATTG CACTGAATAG AACTGTTCTT 
CAGGAAAATG TCAAAGTTAA GTGGAAAAGC AACAGTATTC CTTTACCTAC TGTCGACAAA 
AAAAGTTCTA GACCTATAGG AAAAGGTGTA GAAGGACTTG CAAGAGTTGG ATCCAGAGCA 
GCCCTTTCAT TTGCTTTTGC ATTCCTACGC AGAGCTTGGC GGTCAGGGGA AGATGCCGAT 
CTATGCAGTG AATTGTTACA GGAGTCTCTT GATGCCTTGA GAGCCCTACC AGAAGCATCT 
CTTTTTGATG AAAGCACAGT GTCATCTGTT TGGTTGGAAG TTGTGGAGAG AGCAACTAAA 
TTCCTAAGGT CTGTTGTTAC TGGTGATGTT CATGGAACTG CAAGTACCAA AGGACCAGGA 
AACATTCCAT TACAAGACCA GCACTTAGCA CTTGCCATAT TGTTAGAGTT GGCTGTGCAG 
AGAGGCACTC TAAGCCAAAT GTTATCTGCC ATTATGTTAC TACTTCAATT ATGGGACAAT 
GGGACAAGGG AAACAGATAA TGAACGATCT GCCCAAGGAA CTAGTGCACC ACTTCTCCCT 
TTATTACAAA GATTTCAGAG TATAATCTGC AATAAAGACA TACTTAATAC TGAAGATGAG 
ATTCAGATGC TGTCTGGTCT TCTGAGTCCC AATGAAAGTT TTTTGAGGTA CCTTACTTTA 
CCACAAGACA ATGAATTAGC CATTGATTTG CGTCAAACTG CAGTAGTGAT TATGGCGCAT 
TTAGATCGTC TGGCCACTCC CTGTATGCCT CCTCAGTGTA GTTCTCCAAC ATCTCATAAG 
GGATCTTTAC AAGAAGTCAT AGGTTGGGGC TTAATAGGAT GGAAATACTA TGCTAATGTG 
ATTGGCCCAA TTCAATGTGA AGGACTTGCA AATCTTGGGA TCACACAGAT TGCCTGTGCA 
GAAAAGCGTT TCCTGATTTT GTCTCGGAAT GGGCGAGTTT ACATGCAGGC TTACAACAGT 
GACACATTGG GACCCCAACT AGTTCAAGGT CTTGCCTCTA GAAATATTGT GAAAATTGCA 
GCACATTCAG ATGGTCATCA TTATCTGGCT TTGGCAGCCA CTGGAGAGGT CTTTTCTTGG 
GGCTGTGGAG ATGGTGGACG GTTAGGCCAT GGTGATACAG TGCCTCTAGA AGAACCTAAG 
ATGATATCTG CTCTCTCAGG AAAGCAAGCT GGGAAACATG TAGTTCATAT CGCATGTGGA 
AGTACTTATA GTGCAGCTAT TACAGCTGAA GGAGAGTTAT ATACTTGGGG TCGAGGAAAC 
TATGGCAGGC TGGGACATGG CTCAAGTGAA GATCAGACAA TTCCAATGCT AGTAACTGGA 
CTTAAAGGAC TGAAGGTTAT TGATGTCTCT TGTGGGAGTG GGGATGCTCA GACTCTTGCA 
GTGACTGAGA ATGGTCAAGT CTGGTCTTGG GGCGATGGTG ATTATGGAAA ACTAGGAAGA 
GGTGGTAGTG ATGGTTGTAA AACACCAAAA CTGATTGAAA AACTTCAAGA CTTAGATGTT 
GTTAAAGTAC GTTGTGGAAG CCAATTTTCC GTTGCTTTAA CAAAAGATGG TCAAGTTTAC 
TCGTGGGGAA AAGGTGACAA TCAGAGACTT GGGCATGGAA CAGAGGAGCA TGTTCGATAT 
CCGAAACTGT TAGAAGGCTT ACAAGGTAAA AAAGTCGTTG ATGTGGCTGC TGGTTCTACA 
CACTGCTTAG CACTTACTGA GGATAGCGAA GTTCATAGCT GGGGGAGTAA TGATCAGTGT 
CAGCACTTTG ACACCCTTCG GATTACCAAA CCTGAACCTA CTGCATTGCC AGGATTAGAT 
ACAAAACATA TAGTTGGAAT TGCTTGTGGG CCCGCTCAGA GTTTTGCTTG GTCTTCTTGT 
TCTGAGTGGT CCATTGGCCT ACGTGTACCT TTTGTGGTGG ATGTTTGTCC CATGACATTT 
GAACAGTTGG ATCTCTTACT ACGACAAGTA AGTGAGGGAA TGGATGGCAC CTCTGACTGG 
CCACCACCAC AGGAGAAAGA ATGTATGGCA GTGGCAACAT TGAACCTTCT GAGACTTCAG 
TTGCATGCTG CTATTAGTCA CCAGGTTGAT CCGGAATGTC TTGGATTGGG TCTGGGAAGT 
GTACTCTTGA ATAGCTTGAA GCAGACAGTT GTAACACTAG CAAGTAATGC TGGAGTTCTT 
AATACAGTGC AATCAGCTGC TCAAGCAGTA CTGCAGAGTG GATGGTCTGT GCTGCTTCCA 
ACTGCAGAGG AACGAGCAAG GGCTCTTTCA GCTCTCTTAC CCAGTGCAGT TTCAGGAAAT 
GACATGAATG TAAGTCCAGG ACGTCGTTTT ATGATTGATC TTCTTGTAGG CAGTTTGATG 
GCTGATGGAG GATTAGAATC AGCATTAAAT GCTGCCATCA CTGCAGAAAT CCAGGATATT 
GAAGCAAAAA AAGAAGCTCA AAAGGAAAAA GAGATTGATG AGCAGGAAGC AAATGCTTCA 
ACACTTCATC GGAGTAGGAC ACCATTAGAT AAAGACCTCA TTAATACTGG AATTTATGAA 
TCTGCCGGAA AACAGAGTTT GCCTCTGGTT CAATTAATAC AACAGCTTCT TAGAAATATT 
GCTTCCCAGA CAATAGCCAA ATTGAAAGAT GTTGCTCGTC GAATTTTGTC CTGTATAGAA 
CATGATCAAT ATAGTCAAGA CAGATCTGCC TCCCTGGATT TGTTGCTTCG TTTTCAGCGT 
CTGCTGGTCA GTAAACTCTA TCCAGGAGAC AGTGGTGGTC AAGTTTCAAA TTCCTTTAGT 
CCTGAGCTAT TAGGAGTTGG TTCTTTGCTG AAAAAATATA CAGCTCTTTT GTGCACACAC 
ATTGGAGATA TATTGCCTGT GGCAACTAGT ATTGCATCTT CCAGTTATCG GCATTTTGCA 
GAAGTATCTC GTGTTGTGGA TGGTGATCTA ACAGGTGTTC TTCTTCCAGA GTTGATAGTA 
TCTATTGTCC TTTTGCTCAA TAAAAGTCCT GGATTAATGC AGGAAGCTGG GGCAATTCCT 
CTCTTAGCTG GTCTACTGGA ACATCTAGAT AGATTTAACC ATTTGGCACC AGGAAAAGAG 
AGAGATGATA ATGAGGATTT AGCCTGGCCT GGAATAATGG GATCATTTTT TACAGGTCAG 
AATAGTAGAA ATAATGAGGA AGTGACGCTC ATACGTAAAG CTGATCTGGA GAATCACAAT 
AAAGATGGAG GCTTCTGGAC TGTGATTGAC GGGAAAGTGT ATGATATAAA AGACTTTCAG 
ACACAGTCTT TAACAGGCAA TAGCATTCTT GCTCAGTTTG CAGGTGATGA CCCGGTGGTT 
GCTTTGGAAG CTGCTTTACA GTTTGAGGAC ACAAGGGATT CAATGCATGC ATTTTGTGTT 
GGCCAGTATA TGGAGCCTGA CCAGGAAGTG ATTACAACGC CAGACCTTAC CAGTCTCTCT 
TCACCTCTCA TAGATCCAGA AAGAAATCTG GGTCTCCTCC TTGGACTTCA TGCTTCCTAT 
TTAGCAATGA GCACACCACT ATCTCCTGTG GAGATTGAAT GTGCCAAATG GCTTAAGTCA 
TCCATATTCT CTGGAGGATT GCAAACCAGT CAGATACATT ATAGTTACAA TGAAGAGAAA 
GATGAAGACC ACTGCAGTTC TCCTGGAAAT ACTACTCCAA ACAAATCCAA ACTCTATTCC 
CATAGATTGA CTTTGAGTGA TCATTCCCAA CCATTTTTGC AAGCCATTGC AGATAATAAC 
ATTCAGGATC ATACCGTGAA GGATTTTTTA TGTCAGATAG AAAGGTATTG CAAGCAATGC 
CATTTGACCA CACCGATAAC ATTTCCTCCT GAACATCCTG TGGAAGAAGT GGGACGTTTA 
CTCTTATGTT GTCTTCTAAA GCATGAAGAT TTAGGTCATG TGGCCTTATC TTTAGTTCAC 
CCAGTTACAC TTGGTGTTGA CCAAGTCAAA CACAGAACGT TGCCTAAGTC TGTAGTGGAT 
GTTTGTAGAG TCGTCTACCA GGCAAAATGC TCACTAATAA AGACACATCA GGAACAGGGA 
CGTTCTTATA AGGAAGTGTG TGCTCCTGTT ATTGAACGTT TGCGATTTCT TTTTAATGAA 
TTGCGTCCTG CAGTCTGCAA TGACCTCTCA ATAATGTCCA AACTTAAACT TTTAAGCTCT 
TTGCCTCGTT GGAGAAGAAT AGCTCAGAAG ATGATACGAG AGAGAAGGAA ATTAAAAAGA 
GTACCCAAAA AGTCAGAACC TACTGATGTG GAAGAATCTA AGATTGGCAA TGAAGAGGGT 
GACTTAGAAG AAGCCTGTAT TCTTCCTCAT AGTCCTGTAT CTGTAGATAA AAGACCTGTT 
CCAGTTAAAT CACCTAAGGA TAAATGGCAG CCACTTCTGA GTACAGTTAC AGGTGTTCAC 
AAATACAAAT GGCTTAAACA GAATGTTCAA GGCCTTTATC CTCAGTCTGC ACTGCTTAGC 
ACAATTGTTG AATTTGCGCT TAAAGAAGAG CCAGTTGATG TGGAAAAGAT GAGAAAGTGC 
TTACTCAAAC AGTTGGAGAG AGCAGAAGTT CGTTTGGAAG GAATAGACAC GATTTTAAAA 
TTGGCAAATA AAAATTTCTT GCTTTCGTCT GTGCAATATG CTATGTTCTG TGGATGGCAA 
AGACTTATCC CAGAAGGAAC CAATATAGGG GAACCACTTA CAGATTGTTT AAAGGATGTT 
GATCTGATTC CACCATTTAA TCGAATGCTA CTAGAAGTAA CTTTTGGAAA GTTGTATGGT 
TGGGCCATTC AGAATATTCG AAATGTTTTG TTGGATGCAA ATGCCAGATT TAAAGAGCTA 
GGTATCCAGC CTGTTCCTCT GCAAACTATT ACTAATGAGA ATCCAGCAGG GCCAAGCCTT 
GGAACCATCC CACAAGCACG TTTTCTCCTG GTTATGCTCA ACATGCTTAC CCTTCAATAT 
GGTGCAAACA ACTTGAATCT TCTTTTGAAC TCTGGCATGC TAGCATTGAC TCAGACAACA 
TTGCGATTAA TAGGTCCTAG TTCTGATAAT ATCGAGGAAG AAATGAATGC CTCTGCACGA 
GGAGCTTCAG CTACTGTTTT GGAAGAATCC AGAAAAGAAA CCACTCCAGT ACAACTTCCT 
GTTTCAGGGC CAGAACTGGC AGCAATGATG AAAATTGGTA CCAGGGTCAT GAGGGGAGTG 
GACTGGAAAT GGGGAGATCA GGATGGTCCA CCTCCAGGTT TAGGTCGTGT GATTGGTGAA 
TTGGGAGAGG ATGGATGGAT AAGGGTTCAG TGGGATACAG GCAGTACTAA TTCATACCGA 
ATGGGAAAGG AAGGAAAGTA TGATCTTAAA TTGGCTGAGC CTCCACCTGC AGCTCAGCCC 
TCAACAGAGG ATTCGGACAC TGAGGATGAT TCTGAAAGTG AACAAATGGA AAGAAACTTC 
CACCCTACTT CTATTATGCT TACAAGTACT GTTAATTTAC TCCAAACTCT CTGTCTCTGT 
GCTGGAATTC ATGCAGAAAT CATGCAAAAT GAAGCTACAA GAACTCTGTG TGGTCTACTT 
AGAATGTTAG TGGAAAGTGG AACAGTAGAT AAAACATCTT CTTTACCAAA TAAGATGGTT 
TATAAAGAGC AGCACAGAAG TTGGTGTACA CTTGGATTTA TTCGAAGTAT AGCACTTATG 
CCTCAAATGT GTAGCACCCT TAGCACATCT CAGTGGATCA CATTACTAAT GAAAATTGTG 
GAAGGACACG AGTCATTTAC AGCAGCTTCG CTTCAGAGAC AGATCTTAGC AGTACATTTA 
CTACAAGCTG TACTTCCATC TTGGGACAAA ACAGAAAGAT CAAGAGATAT GAAGTTTCTT 
GTGGAAAAAC TTTTTGGCTT CTTGGGCAGT CTACTTAGTA CATGCTCTTC AGACATACCT 
TTGCTCAGAG AATCAACTCT GAGAAGGAGG AAGTCCAGAC CCCAGGCATC TTTGACTGCA 
ACCCATAGTA GTACTTTGGC AGAGGAGATA GTGGCCCTGC TAAGGACTCT ACATTCACTG 
AGCCAGTGGA ATGGACTCAT AAACAAGTAC ATCAACTCTC AGCTAAACTC CATCACCCAC 
ATCTTTGCAG GAAAACAGTC TGAAGGGACT GTGTTAGAAG ATTACTTTCC TGATTCTGAG 
AACCCTGAAG TTGATGGCCT AATGGCAGTG CTAGCTGTAA TTGGTGGAAT AGATAGTCGA 
TTGAGGCTGG GTGGCCAAGT GGTACATGAT GAATTTGGAG AAGGCACAGT GACACGCATC 
ACTCCTAAAG GCAAAATCAC AGTCCAGTTC TGTGATATGC GAATATGTAG AATTTGTCCA 
TTGAGTCAGC TAAAACCGCT TCCAGTTATG GCATTTAATG TGAACAACTT ACCATTCACG 
GAATCTATGC TCTCTGTCTG GGCTCAATTG GTCAACTTGG CAGGGAGCAA ATTAGAAAAA 
CACAGAATTA AGAAATCTAC TAATCAAGCC CTCACAGGAC AAGTAGACTT GGATTTGCTA 
AGATGCCAAC AGTTGAAATT ATACATATTG AAAGCAGGTC GAGCATTGCT TTCCCATCAG 
GATAAACTGA GGCAGATCTT ATCTCAACCA GCTTTACAAG AGAATGGAGT TCTCTCTACA 
GATGATGGTA CAGCATCCCC TGATATTGGA GATATGTCTC CTGAAGGACC TCAACCTCCA 
ATGATCCTTT TACAGCAATT GTTGACATCT GCCACACAGC CATCACCTGT AAAAGCAATA 
TTTGACAAAC AGGAACTTGA GGCTGCTGCT TTGGCTGTAT GCCAGTGTCT AGCTGTGGAG 
TCAACTCATC CATCAACTCC AGTGTTTGAA GAATGTAGCT CTAGTGAGGC CACAACACCT 
GTCACAGTCC AGCATATTCG ATCTGTAAAA GTGAAAAAGC GAAAGCAGTC TCCTCTCCCT 
GCTTTGCCTA TTGTAGTACA GCTCATGGAA ATGGGATTTC CGAGGAAAAA CATTGAATTT 
GCATTGAAGT CTCTTACTGG GAGTGCTTCA GGGTTACCAG GTGTTGAAGC CTTGGTTGGT 
TGGTTGCTGG ATCATCCTGA TGTCCAAATT ACTGATCTCT CAGATGCTGA TACAGTCTCT 
GATGAATACT CTGATGATGA AATAGTGGAA GATGTGGAAG AGACTGAAGC ATCTTACCCT 
GTGTCTAGTT GTGCTGTTGT AACGGAGAGT CACACTTATA AAAAACGAGC TGATTTCTTA 
AGTAATGATG ACTATGCAGT ATATGTTAGA GAAAACATTC AGGTAGGAAT GATGGTTAGG 
TGCTGCAGAA CGTATGAAGA AGTTTGTGAA GGAGATGTAG GTAAAGTGAT TAAATTGGAC 
AGAGATGGAT TACACGACCT CAATGTGCAG TGTGATTGGC AACAAAAGGG GAGTACCTAT 
TGGGTTAGGT ATATCCATGT TGAACTTATA GGTTTCCCAC CACAGAGTTC TCCTTCCCAT 
ATCAAGATTG GTGATAAAGT TAGGGTGAAA ACATCAGTAA CCACACCAAA ATATAAATGG 
GGATCAGTTA CTCATAGAAG TGTGGGAGTT GTGAAAGCTT TTAGTGCCAA TGGAAAAGAT 
GTTATTGTGG ATTTTCCTCA GCAGTCTCAT TGGACAGGAT TATTGTCAGA AATGGAATTG 
GTGCCTAGTA TTCATCCTGG AGTTACGTGT GATGGCTGCC AAATGTTTCC TATCAATGGT 
CCTCGTTTCA AATGCAGGAG TTGTGATGAT TTTGACTTTT GTGAGACCTG TTTTAAAACC 
AAGAAGCATA ATACCAGACA TAGTTTTGGC AGAATAAATG AACCAGGTCA GTCTCTTGTA 
TTTTGTGGGC GTTCTGGGAA ACAGCTAAAA CGTTGCCACA GTAGTCAGCC TGGAATGCTC 
TTGGATAATT GGTCAAGAAT TGTTAAGAAT TTAAATGTGT CCTCATCAGT AAATCAGGCT 
TCTCGCTTGA TTGATGGCAG TGAACCATGT TGGCAGTCAT CTGGATCACA AGGAAAGCAT 
TGGATTCGAC TGGAGATTTT CCCCGATGTT CTTGTTCATA GACTGAAAAT GATAGTAGAT 
CCTGCTGACA GTAGCTACAT GCCTTCACTA GTTGTAGTTT CAGGTGGGAA TTCCCTAAAT 
AATTTAATTG AACTGAAGAC AATCAATATT AACCCAGCTG ATACCACAGT GCCTCTTCTA 
AATGACTGCA CAGAGTATCA CAGGTATATT GAGGTTGCCA TAAAGCAATG CAGAAGCTCA 
GGAATAGATT GTAAAATTCA TGGTCTTATT ATACTGGGAC GAATTCGGGC AGAAGATGAA 
GATTTGGCTG CTGTTCCTTT CTTAGCGTCA GATAATGAAG AAGAAGATGA TGAAAAAGCT 
AACACTGGGA GTCTTATTAG GAAGAAGGCT TCTGGATTGG AATCAGCAGC CACAATAAGG 
ACCAAAGTTT TTGTCTGGGG GCTGAATGAC AAGGATCAAT TAGGAGGATT AAAGGGTTCT 
AAGATAAAAG TTCCTTCATT CTCTGAAACC CTTTCTGCTT TGAATGTAGT TCAAGTGGCA 
GGTGGATCTA AAAGTTTGTT TGCAGTGACT GTGGAGGGTA AAGTGTATGC TTGTGGAGAA 
GCTACTAATG GACGACTGGG TTTAGGTATT TCTAGTGGAA CTGTACCCAT TCCGCGGCAG 
ATTACAGCTC TCAGCAATTA TGTGGTAAAG AAAGTTGCTG TTCACTCAGG TGGCCGGCAT 
GCTATGGCTT TAACTGTTGA TGGAAAGATA TTTTCATGGG GTGAAGGTGA TGATGGAAAA 
CTAGGACACT TCAGCAGAAT GAACTGTGAT AAACCAAGAC TGATAGAGGC ATTGAAAACC 
AAACGTATTC GTGATATAGC TTGTGGTAGT TCTCACAGTG CTGCCATTAC ATCAAGTGGT 
GAATTGTATA CATGGGGCCT TGGAGAATAT GGCCGGTTGG GACATGGGGA TAATACTACA 
CAGCTGAAAC CCAAAATGGT GAAAGTTCTT CTTGGTCATC GAGTTATTCA AGTTGCTTGT 
GGAAGTAGAG ATGCCCAGAC TCTGGCTTTG ACAGATGAAG GTTTGGTCTT TTCCTGGGGA 
GATGGTGACT TTGGGAAACT GGGCCGGGGA GGAAGTGAAG GATGTAACAT TCCTCAGAAC 
ATTGAGAGAC TTAATGGACA AGGAGTTTGC CAGATTGAGT GTGGGGCTCA ATTTTCCTTG 
GCACTTACAA AGTCAGGAGT AGTATGGACT TGGGGTAAAG GAGATTACTT TAGGCTTGGT 
CATGGCACTG ATGTTCATGT GAGAAAACCA CAAGTAGTAG AAGGACTACG AGGAAAGAAG 
ATAGTTCATG TGGCAGTAGG CGCTCTTCAC TGCTTAGCTG TCACTGATAC AGGTCAAGTG 
TATGCTTGGG GTGACAATGA TCATGGACAA CAAGGCAATG GGACTACTAC TGTCAACAGA 
AAGCCTACCC TTGTACAAGG CTTAGAAGGA CAAAAAATCA CTCGGGTAGC TTGTGGCTCA 
TCTCATAGTG TGGCGTGGAC AACAGTGGAT GTAGCAACCC CATCTGTTCA TGAACCTGTC 
CTCTTTCAAA CTGCTAGGGA CCCTTTAGGC GCTTCTTACT TAGGTGTTCC TTCCGATGCT 
GATTCTTCTG CTGCCAGTAA CAAAATCAGT GGTGTAAATA GTTCCAAGCC TAACCGCCCT 
TCCCTTGCTA AGATTCTCTT GTCTTTGGAT GGAAACCTTG CCAAACAGCA AGCATTATCT 
CATATACTTA CAGCATTGCA AATTATGTAT GCGAGAGATG CAGTAGTTGG AGCATTAATG 
CCTGCTAGTA TGATCGCACC AGTGGAATGC TCATCTTCTT CATCACCTGC ACCCCCCTCA 
GATGTGTCTT CAGCAGGAAG TCCCATGAAT GGTGAAGAGT CTGTGTTAGC TGTGGATGTA 
GAAGATAGAC TAAGCCCAAA TCCATGGCAA GACAAGAGAG GGGAGGTAGT TTCTTCAGAA 
GATGCTGTGA CTCCATCAGC AGTGACTCCC TCAGCTTCCT CTGCCTCTTC TCGACCATTT 
ATTCCAGTAA CTGATGATCC TGGAGCTGCC AGTATCATTG CAGAAACCAT GACTAAAACC 
AAAGAAGATG TTGAAAGCCA AAATAAAGTA TCAGGTCCGG AACCTCAATA TTTGGATGAA 
TTTACTAGTC TCTTAGTGCC AGATGATACC CGTGTCATGG TTGACTTGCT CAAGCTGTCT 
GTTTCTAATC GAGCTGGTGA AAAGGGAAAA GATGTTCTTT CAGCTGTGCT TTCTGGTATG 
GGCACTGCTT ATCCACAGGT AGCTGATATG TTGTTGGAGC TCTGTGTTAC AGAGTTAGAA 
GATGTTGCCA CAGACTCTCA GAGTGGTCGA CTCTCTTCCC AACCTGTTGT AGTAGAAAGT 
AGTCATCCGT ATACTGATGA TACCTCTACC AGTGGTACTG TCAAAATACC AGGTGCTGAA 
GGACTCAGAG TAGAGTTTGA TCGACAATGT TCTACAGAAC GGCGTCATGA CCCTCTCACA 
ATCATGGATG GTGTCAATAG GATAGTTTCA GTTCGATCAG GTCGAGAATG GTCTGATTGG 
TCCAGTGAAC TTCGAATTCC TGGAGATGAA TTAAAGTGGA AGTTCATCAG TGATGGATCA 
GTGAATGGAT GGGGTTGGCG ATTCACTGTC TATCCTATTA TGCCAGCTGC AGGCCCTAAA 
GATCTTCTTT CAGATCGGTG TATTCTTTCC TGTCCCTCAA TGGACTTGGT TACATGCTTG 
TTGGACTTTA GATTAAATTT AGCTTCCAAT AGAAGTATTG TTCCTCGTTT GGCAGCATCA 
CTTGCAGCAT GTGCTCAATT GAGTGCTCTA GCTGCTGGTC ACAGAATGTG GGCCCTTCAA 
AGACTGAGGA AACTGCTTAC AACTGAATTT GGACAATCAA TCAATATAAA TAGGATACTT 
GGAGATAATG ATGGAGAAAC AAGAACCATG AGTTTTACCG GTAGTGCTTT AGCAGCTTTG 
GTAAAAGGTC TTCCAGAAGC TTTGCAAAGA CAGTTTGAAT ATGAAGATCC GATTGTTAGA 
GGAGGCAAAC AACTGCTCCA TAGCCCTTTC TTTAAGGTGC TGGTAGCTCT TGCTTGTGAC 
CTAGAACTGG ATACTCTTCC TTGCTGTGCT GAGACACACA AATGGGCTTG GTTTAGAAGA 
TACTGTATGG CCTCTAGAGT TTCAGTAGCC TTGGACAAAC GAACACCATT ACCTCGCCTT 
TTCCTTGATG AGGTGGCAAA GAAAATCAGG GAGTTAATGG CCGACAGTGA AAATCTGGAT 
GTTCTGCATG AGAGCCATGA CATTTTTAAG AGAGAACAAG ATGAACAACT CGTCCAGTGG 
ATGAACAGGA GACCTGATGA CTGGACTCTT TCTGCTGGAG GCAGTGGAAC AATTTATGGT 
TGGGGTCACA ATCATCGAGG ACAGCTTGGC GGTATTGAGG GAGCAAAAGT CAAAGTTCCA 
ACACCTTGTG AAGCTCTTGC AGCTCTGCGG CCCATACAGT TAATTGGAGG GGAACAGACT 
CTCTTTGCGG TAACTGCTGA TGGGAAGCTT TATGCTACAG GGTATGGTGC AGGTGGCAGA 
TTGGGAATTG GAGGAACTGA ATCAGTTTCT ACTCCAACAT TGCTTGAATC TATTCAGCAT 
GTATTTATAA AAAAAGTTGC TGTGAACTCT GGAGGAAAAC ACTGCTTAGC ACTCTCATCA 
GAAGGAGAAG TTTATTCTTG GGGTGAGGCA GAAGATGGAA AATTGGGGCA TGGAAACAGA 
AGCCCCTGTG ATCGCCCACG TGTAATTGAA TCTTTGCGAG GAATAGAAGT TGTTGATATT 
GCTGCAGGTG GAGCCCACAG TGCATGTATT ACAGCAGCTG GTGACCTCTA TACGTGGGGT 
AAAGGAAGAT ATGGTCGCCT AGGTCATGGG GATAGTGAAG ATCAGTTAAA ACCAAAACTG 
GTGGAAGCTC TCCAAGGCTA TCGAGTTATT GATATTGCCT GTGGCAGTGG AGATGCACAG 
ACACTTTGCC TTACTGATGA TGATACTGTA TGGTCCTGGG GTGATGGGGA CTATGGAAAA 
CTTGGCAGAG GAGGCAGTGA TGGCTGTAAA GTGCCAATGA AGATTGATTC TCTCACAGGT 
CTTGGAGTGG TTAAAGTAGA GTGTGGATCA CAATTTTCTG TTGCACTTAC CAAGTCTGGA 
GCAGTTTATA CTTGGGGTAA AGGCGATTAT CACAGGTTGG GTCATGGATC AGACGACCAT 
GTTAGACGGC CTCGGCAAGT GCAAGGCCTA CAAGGAAAGA AAGTAATAGC AATTGCTACT 
GGTTCTTTAC ACTGTGTGTG CTGCACTGAA GACGGTGAAG TTTATACTTG GGGTGACAAT 
GATGAAGGGC AACTTGGGGA TGGAACAACT AATGCAATCC AGAGACCTCG GTTGGTGGCA 
GCACTTCAGG GTAAAAAGAT AAATAGGGTG GCTTGTGGTT CAGCACACAC CTTAGCGTGG 
TCCACGAGTA AACCTGCCAA TGCTGGGAAG CTTCCAACAC AGGTTCCTAT GGAATACAAC 
CATTTACAGG AATTTCTCAT AATTCCTTTA CGAAACAGAC TTTTGTTATT ACATCATATC 
TCAGAACTCT TTTGTCCATG CATTCCCATG TTTGACCTTG AGGGGCGTCT TGATGAAACT 
GGCCAAGGAC CTTCAGTGGG ATTTGACACA TTACGAGGAA TTCTGATATC TCAGGGAAAG 
GAAGCAGCTT TCCGAAAAGT TGTACAAGCA ACTATGGTGA GAGATCGTCA ACATGGTCCT 
GTAGTTGAAT TAAACAGAAT ACAGGTAAAA CGATCAAGAA GTAAGGGTGG TTTGGCTGGT 
CCTGATGGTA CTAAATCTGT TTTTGGCCAA ATGTGTGCTA AAATGATTTC ATTTAGTCCA 
GACAGTCTTC TTCTACCTCA TCGAGTATGG AAAGTCAAGT TTGTTGGCGA GTCTGTGGAT 
GATTGTGGAG GTGGTTATAG TGAATCAATA GCAGAAATGT GTGAAGAACT TCAGAATGGA 
CTTACTCCTC TCCTAATTGT AACTCCAAAT GGCAGGGATG AATCTGGAGC CAATAGAGAT 
TGCTTCTTAT TGAACCCAGC TGCCAGATCT TCTTTGCATA CCAATATGTT TCGTTTCTTG 
GGTGTATTGC TAGGCATTGC CATTCGAACT GGGAGTCCAT TAAGCCTCAA CCTTGCAGAA 
CCGGTTTGGA AGCAACTGGC AGGAATGAAC CTTACAATTG CTGATCTCAG TGAGGTTGAT 
AAGGATTTTA TTCCTGGCCT GATGTACATT CGGGACAATG AAGCTACTTC TGAGGAATTT 
GAAGCAATGA GCCTTCCCTT CACAGTGCCA AGTGCAAGTG GGCAGGATAT TCAATTGAGC 
TCTAAATATA CCCACATCAC ATTGGATAAC CGTGCTGAGT ATGTTAGATT GGCAATAAAT 
TACAGACTCC ATGAATTTGA TGAACAAGTT GCTGCAGTTC GGGAAGGAAT GGCTCGAGTT 
GTCCCTGTTC CTCTTCTTTC ACTCTTTACT GGATATGAAC TGGAAACTAT GGTGTGTGGA 
AGCCCCGATA TTCCACTTCA TCTTCTAAAA TCTGTAGCCA CATATAAAGG AATTGAACCT 
ACTGCTTCTT TGATCCAGTG GTTCTGGGAA GTGATGGAAT CTTTCTCTAA TACAGAGCGC 
TCTCTCTTTC TTCGTTTTGT GTGGGGCAGA ACACGACTTC CTAGAACAAT TGCTGATTTC 
AGAGGCAGAG ATTTTGTTAT TCAGGTTTTG GATAAGTACA ATCCACCTGA CCACTTCCTT 
CCAGAGTCAT ATACATGCTT CTTTTTGCTG AAGTTACCCA GGTATTCCTG TAAACAAGTA 
CTAGAGGAAA AACTGAAATA TGCCATTCAT TTTTGCAAGT CTATAGACAC TGATGATTAT 
GCCCGGATAG CACTCACAGG AGAACCAGCT GCTGATGACA GCAGTGATGA TTCAGATAAT 
GAAGATGCAG ATTCATTTGC ATCGGACTCT ACTCAAGATT ATTTAACAGG ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023356163.1
CDS	161..14677
Translation	MSSESFCLAAQARFDSKWLKTDLQLAFTRDGLCGLWNEMVKDGEIVYPGTDVIQNGELPPRKDDTMECQSGTKKEDQNDKEKKDEEEIPVPMYKAKSLVESWVWGKQPDVNELKECLYVLVKEQQTLATRMATTTLSAVRLKQRLVILERYFIALNRTVLQENVKVKWKSNSIPLPTVDKKSSRPIGKGVEGLARVGSRAALSFAFAFLRRAWRSGEDADLCSELLQESLDALRALPEASLFDESTVSSVWLEVVERATKFLRSVVTGDVHGTASTKGPGNIPLQDQHLALAILLELAVQRGTLSQMLSAIMLLLQLWDNGTRETDNERSAQGTSAPLLPLLQRFQSIICNKDILNTEDEIQMLSGLLSPNESFLRYLTLPQDNELAIDLRQTAVVIMAHLDRLATPCMPPQCSSPTSHKGSLQEVIGWGLIGWKYYANVIGPIQCEGLANLGITQIACAEKRFLILSRNGRVYMQAYNSDTLGPQLVQGLASRNIVKIAAHSDGHHYLALAATGEVFSWGCGDGGRLGHGDTVPLEEPKMISALSGKQAGKHVVHIACGSTYSAAITAEGELYTWGRGNYGRLGHGSSEDQTIPMLVTGLKGLKVIDVSCGSGDAQTLAVTENGQVWSWGDGDYGKLGRGGSDGCKTPKLIEKLQDLDVVKVRCGSQFSVALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVVDVAAGSTHCLALTEDSEVHSWGSNDQCQHFDTLRITKPEPTALPGLDTKHIVGIACGPAQSFAWSSCSEWSIGLRVPFVVDVCPMTFEQLDLLLRQVSEGMDGTSDWPPPQEKECMAVATLNLLRLQLHAAISHQVDPECLGLGLGSVLLNSLKQTVVTLASNAGVLNTVQSAAQAVLQSGWSVLLPTAEERARALSALLPSAVSGNDMNVSPGRRFMIDLLVGSLMADGGLESALNAAITAEIQDIEAKKEAQKEKEIDEQEANASTLHRSRTPLDKDLINTGIYESAGKQSLPLVQLIQQLLRNIASQTIAKLKDVARRILSCIEHDQYSQDRSASLDLLLRFQRLLVSKLYPGDSGGQVSNSFSPELLGVGSLLKKYTALLCTHIGDILPVATSIASSSYRHFAEVSRVVDGDLTGVLLPELIVSIVLLLNKSPGLMQEAGAIPLLAGLLEHLDRFNHLAPGKERDDNEDLAWPGIMGSFFTGQNSRNNEEVTLIRKADLENHNKDGGFWTVIDGKVYDIKDFQTQSLTGNSILAQFAGDDPVVALEAALQFEDTRDSMHAFCVGQYMEPDQEVITTPDLTSLSSPLIDPERNLGLLLGLHASYLAMSTPLSPVEIECAKWLKSSIFSGGLQTSQIHYSYNEEKDEDHCSSPGNTTPNKSKLYSHRLTLSDHSQPFLQAIADNNIQDHTVKDFLCQIERYCKQCHLTTPITFPPEHPVEEVGRLLLCCLLKHEDLGHVALSLVHPVTLGVDQVKHRTLPKSVVDVCRVVYQAKCSLIKTHQEQGRSYKEVCAPVIERLRFLFNELRPAVCNDLSIMSKLKLLSSLPRWRRIAQKMIRERRKLKRVPKKSEPTDVEESKIGNEEGDLEEACILPHSPVSVDKRPVPVKSPKDKWQPLLSTVTGVHKYKWLKQNVQGLYPQSALLSTIVEFALKEEPVDVEKMRKCLLKQLERAEVRLEGIDTILKLANKNFLLSSVQYAMFCGWQRLIPEGTNIGEPLTDCLKDVDLIPPFNRMLLEVTFGKLYGWAIQNIRNVLLDANARFKELGIQPVPLQTITNENPAGPSLGTIPQARFLLVMLNMLTLQYGANNLNLLLNSGMLALTQTTLRLIGPSSDNIEEEMNASARGASATVLEESRKETTPVQLPVSGPELAAMMKIGTRVMRGVDWKWGDQDGPPPGLGRVIGELGEDGWIRVQWDTGSTNSYRMGKEGKYDLKLAEPPPAAQPSTEDSDTEDDSESEQMERNFHPTSIMLTSTVNLLQTLCLCAGIHAEIMQNEATRTLCGLLRMLVESGTVDKTSSLPNKMVYKEQHRSWCTLGFIRSIALMPQMCSTLSTSQWITLLMKIVEGHESFTAASLQRQILAVHLLQAVLPSWDKTERSRDMKFLVEKLFGFLGSLLSTCSSDIPLLRESTLRRRKSRPQASLTATHSSTLAEEIVALLRTLHSLSQWNGLINKYINSQLNSITHIFAGKQSEGTVLEDYFPDSENPEVDGLMAVLAVIGGIDSRLRLGGQVVHDEFGEGTVTRITPKGKITVQFCDMRICRICPLSQLKPLPVMAFNVNNLPFTESMLSVWAQLVNLAGSKLEKHRIKKSTNQALTGQVDLDLLRCQQLKLYILKAGRALLSHQDKLRQILSQPALQENGVLSTDDGTASPDIGDMSPEGPQPPMILLQQLLTSATQPSPVKAIFDKQELEAAALAVCQCLAVESTHPSTPVFEECSSSEATTPVTVQHIRSVKVKKRKQSPLPALPIVVQLMEMGFPRKNIEFALKSLTGSASGLPGVEALVGWLLDHPDVQITDLSDADTVSDEYSDDEIVEDVEETEASYPVSSCAVVTESHTYKKRADFLSNDDYAVYVRENIQVGMMVRCCRTYEEVCEGDVGKVIKLDRDGLHDLNVQCDWQQKGSTYWVRYIHVELIGFPPQSSPSHIKIGDKVRVKTSVTTPKYKWGSVTHRSVGVVKAFSANGKDVIVDFPQQSHWTGLLSEMELVPSIHPGVTCDGCQMFPINGPRFKCRSCDDFDFCETCFKTKKHNTRHSFGRINEPGQSLVFCGRSGKQLKRCHSSQPGMLLDNWSRIVKNLNVSSSVNQASRLIDGSEPCWQSSGSQGKHWIRLEIFPDVLVHRLKMIVDPADSSYMPSLVVVSGGNSLNNLIELKTININPADTTVPLLNDCTEYHRYIEVAIKQCRSSGIDCKIHGLIILGRIRAEDEDLAAVPFLASDNEEEDDEKANTGSLIRKKASGLESAATIRTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALNVVQVAGGSKSLFAVTVEGKVYACGEATNGRLGLGISSGTVPIPRQITALSNYVVKKVAVHSGGRHAMALTVDGKIFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAAITSSGELYTWGLGEYGRLGHGDNTTQLKPKMVKVLLGHRVIQVACGSRDAQTLALTDEGLVFSWGDGDFGKLGRGGSEGCNIPQNIERLNGQGVCQIECGAQFSLALTKSGVVWTWGKGDYFRLGHGTDVHVRKPQVVEGLRGKKIVHVAVGALHCLAVTDTGQVYAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSVAWTTVDVATPSVHEPVLFQTARDPLGASYLGVPSDADSSAASNKISGVNSSKPNRPSLAKILLSLDGNLAKQQALSHILTALQIMYARDAVVGALMPASMIAPVECSSSSSPAPPSDVSSAGSPMNGEESVLAVDVEDRLSPNPWQDKRGEVVSSEDAVTPSAVTPSASSASSRPFIPVTDDPGAASIIAETMTKTKEDVESQNKVSGPEPQYLDEFTSLLVPDDTRVMVDLLKLSVSNRAGEKGKDVLSAVLSGMGTAYPQVADMLLELCVTELEDVATDSQSGRLSSQPVVVESSHPYTDDTSTSGTVKIPGAEGLRVEFDRQCSTERRHDPLTIMDGVNRIVSVRSGREWSDWSSELRIPGDELKWKFISDGSVNGWGWRFTVYPIMPAAGPKDLLSDRCILSCPSMDLVTCLLDFRLNLASNRSIVPRLAASLAACAQLSALAAGHRMWALQRLRKLLTTEFGQSININRILGDNDGETRTMSFTGSALAALVKGLPEALQRQFEYEDPIVRGGKQLLHSPFFKVLVALACDLELDTLPCCAETHKWAWFRRYCMASRVSVALDKRTPLPRLFLDEVAKKIRELMADSENLDVLHESHDIFKREQDEQLVQWMNRRPDDWTLSAGGSGTIYGWGHNHRGQLGGIEGAKVKVPTPCEALAALRPIQLIGGEQTLFAVTADGKLYATGYGAGGRLGIGGTESVSTPTLLESIQHVFIKKVAVNSGGKHCLALSSEGEVYSWGEAEDGKLGHGNRSPCDRPRVIESLRGIEVVDIAAGGAHSACITAAGDLYTWGKGRYGRLGHGDSEDQLKPKLVEALQGYRVIDIACGSGDAQTLCLTDDDTVWSWGDGDYGKLGRGGSDGCKVPMKIDSLTGLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLGHGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCTEDGEVYTWGDNDEGQLGDGTTNAIQRPRLVAALQGKKINRVACGSAHTLAWSTSKPANAGKLPTQVPMEYNHLQEFLIIPLRNRLLLLHHISELFCPCIPMFDLEGRLDETGQGPSVGFDTLRGILISQGKEAAFRKVVQATMVRDRQHGPVVELNRIQVKRSRSKGGLAGPDGTKSVFGQMCAKMISFSPDSLLLPHRVWKVKFVGESVDDCGGGYSESIAEMCEELQNGLTPLLIVTPNGRDESGANRDCFLLNPAARSSLHTNMFRFLGVLLGIAIRTGSPLSLNLAEPVWKQLAGMNLTIADLSEVDKDFIPGLMYIRDNEATSEEFEAMSLPFTVPSASGQDIQLSSKYTHITLDNRAEYVRLAINYRLHEFDEQVAAVREGMARVVPVPLLSLFTGYELETMVCGSPDIPLHLLKSVATYKGIEPTASLIQWFWEVMESFSNTERSLFLRFVWGRTRLPRTIADFRGRDFVIQVLDKYNPPDHFLPESYTCFFLLKLPRYSCKQVLEEKLKYAIHFCKSIDTDDYARIALTGEPAADDSSDDSDNEDADSFASDSTQDYLTGH

Target ORF information:

RefSeq Version	XM_023500395.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HECT and RLD domain containing E3 ubiquitin protein ligase 2 (HERC2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023500395.1

ORF Insert Sequence:

ATGTCTTCTG AATCCTTCTG TTTAGCTGCC CAGGCTAGAT TTGACTCCAA GTGGTTAAAA 
ACAGATTTAC AGCTTGCTTT TACAAGAGAT GGACTCTGTG GCCTATGGAA TGAAATGGTC 
AAAGATGGAG AAATTGTATA TCCTGGGACA GATGTAATAC AGAATGGAGA ACTACCTCCT 
CGAAAAGATG ACACCATGGA ATGTCAGAGT GGAACAAAGA AAGAAGATCA AAATGATAAA 
GAGAAGAAAG ATGAAGAGGA AATTCCAGTC CCTATGTATA AGGCCAAATC ACTTGTGGAG 
AGTTGGGTAT GGGGCAAACA ACCAGATGTG AACGAGTTGA AAGAGTGCCT TTATGTGCTA 
GTGAAAGAAC AGCAAACTCT TGCTACACGG ATGGCTACAA CAACTCTTTC AGCAGTAAGG 
CTAAAGCAGA GGCTGGTTAT ACTGGAACGA TATTTCATTG CACTGAATAG AACTGTTCTT 
CAGGAAAATG TCAAAGTTAA GTGGAAAAGC AACAGTATTC CTTTACCTAC TGTCGACAAA 
AAAAGTTCTA GACCTATAGG AAAAGGTGTA GAAGGACTTG CAAGAGTTGG ATCCAGAGCA 
GCCCTTTCAT TTGCTTTTGC ATTCCTACGC AGAGCTTGGC GGTCAGGGGA AGATGCCGAT 
CTATGCAGTG AATTGTTACA GGAGTCTCTT GATGCCTTGA GAGCCCTACC AGAAGCATCT 
CTTTTTGATG AAAGCACAGT GTCATCTGTT TGGTTGGAAG TTGTGGAGAG AGCAACTAAA 
TTCCTAAGGT CTGTTGTTAC TGGTGATGTT CATGGAACTG CAAGTACCAA AGGACCAGGA 
AACATTCCAT TACAAGACCA GCACTTAGCA CTTGCCATAT TGTTAGAGTT GGCTGTGCAG 
AGAGGCACTC TAAGCCAAAT GTTATCTGCC ATTATGTTAC TACTTCAATT ATGGGACAAT 
GGGACAAGGG AAACAGATAA TGAACGATCT GCCCAAGGAA CTAGTGCACC ACTTCTCCCT 
TTATTACAAA GATTTCAGAG TATAATCTGC AATAAAGACA TACTTAATAC TGAAGATGAG 
ATTCAGATGC TGTCTGGTCT TCTGAGTCCC AATGAAAGTT TTTTGAGGTA CCTTACTTTA 
CCACAAGACA ATGAATTAGC CATTGATTTG CGTCAAACTG CAGTAGTGAT TATGGCGCAT 
TTAGATCGTC TGGCCACTCC CTGTATGCCT CCTCAGTGTA GTTCTCCAAC ATCTCATAAG 
GGATCTTTAC AAGAAGTCAT AGGTTGGGGC TTAATAGGAT GGAAATACTA TGCTAATGTG 
ATTGGCCCAA TTCAATGTGA AGGACTTGCA AATCTTGGGA TCACACAGAT TGCCTGTGCA 
GAAAAGCGTT TCCTGATTTT GTCTCGGAAT GGGCGAGTTT ACATGCAGGC TTACAACAGT 
GACACATTGG GACCCCAACT AGTTCAAGGT CTTGCCTCTA GAAATATTGT GAAAATTGCA 
GCACATTCAG ATGGTCATCA TTATCTGGCT TTGGCAGCCA CTGGAGAGGT CTTTTCTTGG 
GGCTGTGGAG ATGGTGGACG GTTAGGCCAT GGTGATACAG TGCCTCTAGA AGAACCTAAG 
ATGATATCTG CTCTCTCAGG AAAGCAAGCT GGGAAACATG TAGTTCATAT CGCATGTGGA 
AGTACTTATA GTGCAGCTAT TACAGCTGAA GGAGAGTTAT ATACTTGGGG TCGAGGAAAC 
TATGGCAGGC TGGGACATGG CTCAAGTGAA GATCAGACAA TTCCAATGCT AGTAACTGGA 
CTTAAAGGAC TGAAGGTTAT TGATGTCTCT TGTGGGAGTG GGGATGCTCA GACTCTTGCA 
GTGACTGAGA ATGGTCAAGT CTGGTCTTGG GGCGATGGTG ATTATGGAAA ACTAGGAAGA 
GGTGGTAGTG ATGGTTGTAA AACACCAAAA CTGATTGAAA AACTTCAAGA CTTAGATGTT 
GTTAAAGTAC GTTGTGGAAG CCAATTTTCC GTTGCTTTAA CAAAAGATGG TCAAGTTTAC 
TCGTGGGGAA AAGGTGACAA TCAGAGACTT GGGCATGGAA CAGAGGAGCA TGTTCGATAT 
CCGAAACTGT TAGAAGGCTT ACAAGGTAAA AAAGTCGTTG ATGTGGCTGC TGGTTCTACA 
CACTGCTTAG CACTTACTGA GGATAGCGAA GTTCATAGCT GGGGGAGTAA TGATCAGTGT 
CAGCACTTTG ACACCCTTCG GATTACCAAA CCTGAACCTA CTGCATTGCC AGGATTAGAT 
ACAAAACATA TAGTTGGAAT TGCTTGTGGG CCCGCTCAGA GTTTTGCTTG GTCTTCTTGT 
TCTGAGTGGT CCATTGGCCT ACGTGTACCT TTTGTGGTGG ATGTTTGTCC CATGACATTT 
GAACAGTTGG ATCTCTTACT ACGACAAGTA AGTGAGGGAA TGGATGGCAC CTCTGACTGG 
CCACCACCAC AGGAGAAAGA ATGTATGGCA GTGGCAACAT TGAACCTTCT GAGACTTCAG 
TTGCATGCTG CTATTAGTCA CCAGGTTGAT CCGGAATGTC TTGGATTGGG TCTGGGAAGT 
GTACTCTTGA ATAGCTTGAA GCAGACAGTT GTAACACTAG CAAGTAATGC TGGAGTTCTT 
AATACAGTGC AATCAGCTGC TCAAGCAGTA CTGCAGAGTG GATGGTCTGT GCTGCTTCCA 
ACTGCAGAGG AACGAGCAAG GGCTCTTTCA GCTCTCTTAC CCAGTGCAGT TTCAGGAAAT 
GACATGAATG TAAGTCCAGG ACGTCGTTTT ATGATTGATC TTCTTGTAGG CAGTTTGATG 
GCTGATGGAG GATTAGAATC AGCATTAAAT GCTGCCATCA CTGCAGAAAT CCAGGATATT 
GAAGCAAAAA AAGAAGCTCA AAAGGAAAAA GAGATTGATG AGCAGGAAGC AAATGCTTCA 
ACACTTCATC GGAGTAGGAC ACCATTAGAT AAAGACCTCA TTAATACTGG AATTTATGAA 
TCTGCCGGAA AACAGAGTTT GCCTCTGGTT CAATTAATAC AACAGCTTCT TAGAAATATT 
GCTTCCCAGA CAATAGCCAA ATTGAAAGAT GTTGCTCGTC GAATTTTGTC CTGTATAGAA 
CATGATCAAT ATAGTCAAGA CAGATCTGCC TCCCTGGATT TGTTGCTTCG TTTTCAGCGT 
CTGCTGGTCA GTAAACTCTA TCCAGGAGAC AGTGGTGGTC AAGTTTCAAA TTCCTTTAGT 
CCTGAGCTAT TAGGAGTTGG TTCTTTGCTG AAAAAATATA CAGCTCTTTT GTGCACACAC 
ATTGGAGATA TATTGCCTGT GGCAACTAGT ATTGCATCTT CCAGTTATCG GCATTTTGCA 
GAAGTATCTC GTGTTGTGGA TGGTGATCTA ACAGGTGTTC TTCTTCCAGA GTTGATAGTA 
TCTATTGTCC TTTTGCTCAA TAAAAGTCCT GGATTAATGC AGGAAGCTGG GGCAATTCCT 
CTCTTAGCTG GTCTACTGGA ACATCTAGAT AGATTTAACC ATTTGGCACC AGGAAAAGAG 
AGAGATGATA ATGAGGATTT AGCCTGGCCT GGAATAATGG GATCATTTTT TACAGGTCAG 
AATAGTAGAA ATAATGAGGA AGTGACGCTC ATACGTAAAG CTGATCTGGA GAATCACAAT 
AAAGATGGAG GCTTCTGGAC TGTGATTGAC GGGAAAGTGT ATGATATAAA AGACTTTCAG 
ACACAGTCTT TAACAGGCAA TAGCATTCTT GCTCAGTTTG CAGGTGATGA CCCGGTGGTT 
GCTTTGGAAG CTGCTTTACA GTTTGAGGAC ACAAGGGATT CAATGCATGC ATTTTGTGTT 
GGCCAGTATA TGGAGCCTGA CCAGGAAGTG ATTACAACGC CAGACCTTAC CAGTCTCTCT 
TCACCTCTCA TAGATCCAGA AAGAAATCTG GGTCTCCTCC TTGGACTTCA TGCTTCCTAT 
TTAGCAATGA GCACACCACT ATCTCCTGTG GAGATTGAAT GTGCCAAATG GCTTAAGTCA 
TCCATATTCT CTGGAGGATT GCAAACCAGT CAGATACATT ATAGTTACAA TGAAGAGAAA 
GATGAAGACC ACTGCAGTTC TCCTGGAAAT ACTACTCCAA ACAAATCCAA ACTCTATTCC 
CATAGATTGA CTTTGAGTGA TCATTCCCAA CCATTTTTGC AAGCCATTGC AGATAATAAC 
ATTCAGGATC ATACCGTGAA GGATTTTTTA TGTCAGATAG AAAGGTATTG CAAGCAATGC 
CATTTGACCA CACCGATAAC ATTTCCTCCT GAACATCCTG TGGAAGAAGT GGGACGTTTA 
CTCTTATGTT GTCTTCTAAA GCATGAAGAT TTAGGTCATG TGGCCTTATC TTTAGTTCAC 
CCAGTTACAC TTGGTGTTGA CCAAGTCAAA CACAGAACGT TGCCTAAGTC TGTAGTGGAT 
GTTTGTAGAG TCGTCTACCA GGCAAAATGC TCACTAATAA AGACACATCA GGAACAGGGA 
CGTTCTTATA AGGAAGTGTG TGCTCCTGTT ATTGAACGTT TGCGATTTCT TTTTAATGAA 
TTGCGTCCTG CAGTCTGCAA TGACCTCTCA ATAATGTCCA AACTTAAACT TTTAAGCTCT 
TTGCCTCGTT GGAGAAGAAT AGCTCAGAAG ATGATACGAG AGAGAAGGAA ATTAAAAAGA 
GTACCCAAAA AGTCAGAACC TACTGATGTG GAAGAATCTA AGATTGGCAA TGAAGAGGGT 
GACTTAGAAG AAGCCTGTAT TCTTCCTCAT AGTCCTGTAT CTGTAGATAA AAGACCTGTT 
CCAGTTAAAT CACCTAAGGA TAAATGGCAG CCACTTCTGA GTACAGTTAC AGGTGTTCAC 
AAATACAAAT GGCTTAAACA GAATGTTCAA GGCCTTTATC CTCAGTCTGC ACTGCTTAGC 
ACAATTGTTG AATTTGCGCT TAAAGAAGAG CCAGTTGATG TGGAAAAGAT GAGAAAGTGC 
TTACTCAAAC AGTTGGAGAG AGCAGAAGTT CGTTTGGAAG GAATAGACAC GATTTTAAAA 
TTGGCAAATA AAAATTTCTT GCTTTCGTCT GTGCAATATG CTATGTTCTG TGGATGGCAA 
AGACTTATCC CAGAAGGAAC CAATATAGGG GAACCACTTA CAGATTGTTT AAAGGATGTT 
GATCTGATTC CACCATTTAA TCGAATGCTA CTAGAAGTAA CTTTTGGAAA GTTGTATGGT 
TGGGCCATTC AGAATATTCG AAATGTTTTG TTGGATGCAA ATGCCAGATT TAAAGAGCTA 
GGTATCCAGC CTGTTCCTCT GCAAACTATT ACTAATGAGA ATCCAGCAGG GCCAAGCCTT 
GGAACCATCC CACAAGCACG TTTTCTCCTG GTTATGCTCA ACATGCTTAC CCTTCAATAT 
GGTGCAAACA ACTTGAATCT TCTTTTGAAC TCTGGCATGC TAGCATTGAC TCAGACAACA 
TTGCGATTAA TAGGTCCTAG TTCTGATAAT ATCGAGGAAG AAATGAATGC CTCTGCACGA 
GGAGCTTCAG CTACTGTTTT GGAAGAATCC AGAAAAGAAA CCACTCCAGT ACAACTTCCT 
GTTTCAGGGC CAGAACTGGC AGCAATGATG AAAATTGGTA CCAGGGTCAT GAGGGGAGTG 
GACTGGAAAT GGGGAGATCA GGATGGTCCA CCTCCAGGTT TAGGTCGTGT GATTGGTGAA 
TTGGGAGAGG ATGGATGGAT AAGGGTTCAG TGGGATACAG GCAGTACTAA TTCATACCGA 
ATGGGAAAGG AAGGAAAGTA TGATCTTAAA TTGGCTGAGC CTCCACCTGC AGCTCAGCCC 
TCAACAGAGG ATTCGGACAC TGAGGATGAT TCTGAAAGTG AACAAATGGA AAGAAACTTC 
CACCCTACTT CTATTATGCT TACAAGTACT GTTAATTTAC TCCAAACTCT CTGTCTCTGT 
GCTGGAATTC ATGCAGAAAT CATGCAAAAT GAAGCTACAA GAACTCTGTG TGGTCTACTT 
AGAATGTTAG TGGAAAGTGG AACAGTAGAT AAAACATCTT CTTTACCAAA TAAGATGGTT 
TATAAAGAGC AGCACAGAAG TTGGTGTACA CTTGGATTTA TTCGAAGTAT AGCACTTATG 
CCTCAAATGT GTAGCACCCT TAGCACATCT CAGTGGATCA CATTACTAAT GAAAATTGTG 
GAAGGACACG AGTCATTTAC AGCAGCTTCG CTTCAGAGAC AGATCTTAGC AGTACATTTA 
CTACAAGCTG TACTTCCATC TTGGGACAAA ACAGAAAGAT CAAGAGATAT GAAGTTTCTT 
GTGGAAAAAC TTTTTGGCTT CTTGGGCAGT CTACTTAGTA CATGCTCTTC AGACATACCT 
TTGCTCAGAG AATCAACTCT GAGAAGGAGG AAGTCCAGAC CCCAGGCATC TTTGACTGCA 
ACCCATAGTA GTACTTTGGC AGAGGAGATA GTGGCCCTGC TAAGGACTCT ACATTCACTG 
AGCCAGTGGA ATGGACTCAT AAACAAGTAC ATCAACTCTC AGCTAAACTC CATCACCCAC 
ATCTTTGCAG GAAAACAGTC TGAAGGGACT GTGTTAGAAG ATTACTTTCC TGATTCTGAG 
AACCCTGAAG TTGATGGCCT AATGGCAGTG CTAGCTGTAA TTGGTGGAAT AGATAGTCGA 
TTGAGGCTGG GTGGCCAAGT GGTACATGAT GAATTTGGAG AAGGCACAGT GACACGCATC 
ACTCCTAAAG GCAAAATCAC AGTCCAGTTC TGTGATATGC GAATATGTAG AATTTGTCCA 
TTGAGTCAGC TAAAACCGCT TCCAGTTATG GCATTTAATG TGAACAACTT ACCATTCACG 
GAATCTATGC TCTCTGTCTG GGCTCAATTG GTCAACTTGG CAGGGAGCAA ATTAGAAAAA 
CACAGAATTA AGAAATCTAC TAATCAAGCC CTCACAGGAC AAGTAGACTT GGATTTGCTA 
AGATGCCAAC AGTTGAAATT ATACATATTG AAAGCAGGTC GAGCATTGCT TTCCCATCAG 
GATAAACTGA GGCAGATCTT ATCTCAACCA GCTTTACAAG AGAATGGAGT TCTCTCTACA 
GATGATGGTA CAGCATCCCC TGATATTGGA GATATGTCTC CTGAAGGACC TCAACCTCCA 
ATGATCCTTT TACAGCAATT GTTGACATCT GCCACACAGC CATCACCTGT AAAAGCAATA 
TTTGACAAAC AGGAACTTGA GGCTGCTGCT TTGGCTGTAT GCCAGTGTCT AGCTGTGGAG 
TCAACTCATC CATCAACTCC AGTGTTTGAA GAATGTAGCT CTAGTGAGGC CACAACACCT 
GTCACAGTCC AGCATATTCG ATCTGTAAAA GTGAAAAAGC GAAAGCAGTC TCCTCTCCCT 
GCTTTGCCTA TTGTAGTACA GCTCATGGAA ATGGGATTTC CGAGGAAAAA CATTGAATTT 
GCATTGAAGT CTCTTACTGG GAGTGCTTCA GGGTTACCAG GTGTTGAAGC CTTGGTTGGT 
TGGTTGCTGG ATCATCCTGA TGTCCAAATT ACTGATCTCT CAGATGCTGA TACAGTCTCT 
GATGAATACT CTGATGATGA AATAGTGGAA GATGTGGAAG AGACTGAAGC ATCTTACCCT 
GTGTCTAGTT GTGCTGTTGT AACGGAGAGT CACACTTATA AAAAACGAGC TGATTTCTTA 
AGTAATGATG ACTATGCAGT ATATGTTAGA GAAAACATTC AGGTAGGAAT GATGGTTAGG 
TGCTGCAGAA CGTATGAAGA AGTTTGTGAA GGAGATGTAG GTAAAGTGAT TAAATTGGAC 
AGAGATGGAT TACACGACCT CAATGTGCAG TGTGATTGGC AACAAAAGGG GAGTACCTAT 
TGGGTTAGGT ATATCCATGT TGAACTTATA GGTTTCCCAC CACAGAGTTC TCCTTCCCAT 
ATCAAGATTG GTGATAAAGT TAGGGTGAAA ACATCAGTAA CCACACCAAA ATATAAATGG 
GGATCAGTTA CTCATAGAAG TGTGGGAGTT GTGAAAGCTT TTAGTGCCAA TGGAAAAGAT 
GTTATTGTGG ATTTTCCTCA GCAGTCTCAT TGGACAGGAT TATTGTCAGA AATGGAATTG 
GTGCCTAGTA TTCATCCTGG AGTTACGTGT GATGGCTGCC AAATGTTTCC TATCAATGGT 
CCTCGTTTCA AATGCAGGAG TTGTGATGAT TTTGACTTTT GTGAGACCTG TTTTAAAACC 
AAGAAGCATA ATACCAGACA TAGTTTTGGC AGAATAAATG AACCAGGTCA GTCTCTTGTA 
TTTTGTGGGC GTTCTGGGAA ACAGCTAAAA CGTTGCCACA GTAGTCAGCC TGGAATGCTC 
TTGGATAATT GGTCAAGAAT TGTTAAGAAT TTAAATGTGT CCTCATCAGT AAATCAGGCT 
TCTCGCTTGA TTGATGGCAG TGAACCATGT TGGCAGTCAT CTGGATCACA AGGAAAGCAT 
TGGATTCGAC TGGAGATTTT CCCCGATGTT CTTGTTCATA GACTGAAAAT GATAGTAGAT 
CCTGCTGACA GTAGCTACAT GCCTTCACTA GTTGTAGTTT CAGGTGGGAA TTCCCTAAAT 
AATTTAATTG AACTGAAGAC AATCAATATT AACCCAGCTG ATACCACAGT GCCTCTTCTA 
AATGACTGCA CAGAGTATCA CAGGTATATT GAGGTTGCCA TAAAGCAATG CAGAAGCTCA 
GGAATAGATT GTAAAATTCA TGGTCTTATT ATACTGGGAC GAATTCGGGC AGAAGATGAA 
GATTTGGCTG CTGTTCCTTT CTTAGCGTCA GATAATGAAG AAGAAGATGA TGAAAAAGCT 
AACACTGGGA GTCTTATTAG GAAGAAGGCT TCTGGATTGG AATCAGCAGC CACAATAAGG 
ACCAAAGTTT TTGTCTGGGG GCTGAATGAC AAGGATCAAT TAGGAGGATT AAAGGGTTCT 
AAGATAAAAG TTCCTTCATT CTCTGAAACC CTTTCTGCTT TGAATGTAGT TCAAGTGGCA 
GGTGGATCTA AAAGTTTGTT TGCAGTGACT GTGGAGGGTA AAGTGTATGC TTGTGGAGAA 
GCTACTAATG GACGACTGGG TTTAGGTATT TCTAGTGGAA CTGTACCCAT TCCGCGGCAG 
ATTACAGCTC TCAGCAATTA TGTGGTAAAG AAAGTTGCTG TTCACTCAGG TGGCCGGCAT 
GCTATGGCTT TAACTGTTGA TGGAAAGATA TTTTCATGGG GTGAAGGTGA TGATGGAAAA 
CTAGGACACT TCAGCAGAAT GAACTGTGAT AAACCAAGAC TGATAGAGGC ATTGAAAACC 
AAACGTATTC GTGATATAGC TTGTGGTAGT TCTCACAGTG CTGCCATTAC ATCAAGTGGT 
GAATTGTATA CATGGGGCCT TGGAGAATAT GGCCGGTTGG GACATGGGGA TAATACTACA 
CAGCTGAAAC CCAAAATGGT GAAAGTTCTT CTTGGTCATC GAGTTATTCA AGTTGCTTGT 
GGAAGTAGAG ATGCCCAGAC TCTGGCTTTG ACAGATGAAG GTTTGGTCTT TTCCTGGGGA 
GATGGTGACT TTGGGAAACT GGGCCGGGGA GGAAGTGAAG GATGTAACAT TCCTCAGAAC 
ATTGAGAGAC TTAATGGACA AGGAGTTTGC CAGATTGAGT GTGGGGCTCA ATTTTCCTTG 
GCACTTACAA AGTCAGGAGT AGTATGGACT TGGGGTAAAG GAGATTACTT TAGGCTTGGT 
CATGGCACTG ATGTTCATGT GAGAAAACCA CAAGTAGTAG AAGGACTACG AGGAAAGAAG 
ATAGTTCATG TGGCAGTAGG CGCTCTTCAC TGCTTAGCTG TCACTGATAC AGGTCAAGTG 
TATGCTTGGG GTGACAATGA TCATGGACAA CAAGGCAATG GGACTACTAC TGTCAACAGA 
AAGCCTACCC TTGTACAAGG CTTAGAAGGA CAAAAAATCA CTCGGGTAGC TTGTGGCTCA 
TCTCATAGTG TGGCGTGGAC AACAGTGGAT GTAGCAACCC CATCTGTTCA TGAACCTGTC 
CTCTTTCAAA CTGCTAGGGA CCCTTTAGGC GCTTCTTACT TAGGTGTTCC TTCCGATGCT 
GATTCTTCTG CTGCCAGTAA CAAAATCAGT GGTGTAAATA GTTCCAAGCC TAACCGCCCT 
TCCCTTGCTA AGATTCTCTT GTCTTTGGAT GGAAACCTTG CCAAACAGCA AGCATTATCT 
CATATACTTA CAGCATTGCA AATTATGTAT GCGAGAGATG CAGTAGTTGG AGCATTAATG 
CCTGCTAGTA TGATCGCACC AGTGGAATGC TCATCTTCTT CATCACCTGC ACCCCCCTCA 
GATGTGTCTT CAGCAGGAAG TCCCATGAAT GGTGAAGAGT CTGTGTTAGC TGTGGATGTA 
GAAGATAGAC TAAGCCCAAA TCCATGGCAA GACAAGAGAG GGGAGGTAGT TTCTTCAGAA 
GATGCTGTGA CTCCATCAGC AGTGACTCCC TCAGCTTCCT CTGCCTCTTC TCGACCATTT 
ATTCCAGTAA CTGATGATCC TGGAGCTGCC AGTATCATTG CAGAAACCAT GACTAAAACC 
AAAGAAGATG TTGAAAGCCA AAATAAAGTA TCAGGTCCGG AACCTCAATA TTTGGATGAA 
TTTACTAGTC TCTTAGTGCC AGATGATACC CGTGTCATGG TTGACTTGCT CAAGCTGTCT 
GTTTCTAATC GAGCTGGTGA AAAGGGAAAA GATGTTCTTT CAGCTGTGCT TTCTGGTATG 
GGCACTGCTT ATCCACAGGT AGCTGATATG TTGTTGGAGC TCTGTGTTAC AGAGTTAGAA 
GATGTTGCCA CAGACTCTCA GAGTGGTCGA CTCTCTTCCC AACCTGTTGT AGTAGAAAGT 
AGTCATCCGT ATACTGATGA TACCTCTACC AGTGGTACTG TCAAAATACC AGGTGCTGAA 
GGACTCAGAG TAGAGTTTGA TCGACAATGT TCTACAGAAC GGCGTCATGA CCCTCTCACA 
ATCATGGATG GTGTCAATAG GATAGTTTCA GTTCGATCAG GTCGAGAATG GTCTGATTGG 
TCCAGTGAAC TTCGAATTCC TGGAGATGAA TTAAAGTGGA AGTTCATCAG TGATGGATCA 
GTGAATGGAT GGGGTTGGCG ATTCACTGTC TATCCTATTA TGCCAGCTGC AGGCCCTAAA 
GATCTTCTTT CAGATCGGTG TATTCTTTCC TGTCCCTCAA TGGACTTGGT TACATGCTTG 
TTGGACTTTA GATTAAATTT AGCTTCCAAT AGAAGTATTG TTCCTCGTTT GGCAGCATCA 
CTTGCAGCAT GTGCTCAATT GAGTGCTCTA GCTGCTGGTC ACAGAATGTG GGCCCTTCAA 
AGACTGAGGA AACTGCTTAC AACTGAATTT GGACAATCAA TCAATATAAA TAGGATACTT 
GGAGATAATG ATGGAGAAAC AAGAACCATG AGTTTTACCG GTAGTGCTTT AGCAGCTTTG 
GTAAAAGGTC TTCCAGAAGC TTTGCAAAGA CAGTTTGAAT ATGAAGATCC GATTGTTAGA 
GGAGGCAAAC AACTGCTCCA TAGCCCTTTC TTTAAGGTGC TGGTAGCTCT TGCTTGTGAC 
CTAGAACTGG ATACTCTTCC TTGCTGTGCT GAGACACACA AATGGGCTTG GTTTAGAAGA 
TACTGTATGG CCTCTAGAGT TTCAGTAGCC TTGGACAAAC GAACACCATT ACCTCGCCTT 
TTCCTTGATG AGGTGGCAAA GAAAATCAGG GAGTTAATGG CCGACAGTGA AAATCTGGAT 
GTTCTGCATG AGAGCCATGA CATTTTTAAG AGAGAACAAG ATGAACAACT CGTCCAGTGG 
ATGAACAGGA GACCTGATGA CTGGACTCTT TCTGCTGGAG GCAGTGGAAC AATTTATGGT 
TGGGGTCACA ATCATCGAGG ACAGCTTGGC GGTATTGAGG GAGCAAAAGT CAAAGTTCCA 
ACACCTTGTG AAGCTCTTGC AGCTCTGCGG CCCATACAGT TAATTGGAGG GGAACAGACT 
CTCTTTGCGG TAACTGCTGA TGGGAAGCTT TATGCTACAG GGTATGGTGC AGGTGGCAGA 
TTGGGAATTG GAGGAACTGA ATCAGTTTCT ACTCCAACAT TGCTTGAATC TATTCAGCAT 
GTATTTATAA AAAAAGTTGC TGTGAACTCT GGAGGAAAAC ACTGCTTAGC ACTCTCATCA 
GAAGGAGAAG TTTATTCTTG GGGTGAGGCA GAAGATGGAA AATTGGGGCA TGGAAACAGA 
AGCCCCTGTG ATCGCCCACG TGTAATTGAA TCTTTGCGAG GAATAGAAGT TGTTGATATT 
GCTGCAGGTG GAGCCCACAG TGCATGTATT ACAGCAGCTG GTGACCTCTA TACGTGGGGT 
AAAGGAAGAT ATGGTCGCCT AGGTCATGGG GATAGTGAAG ATCAGTTAAA ACCAAAACTG 
GTGGAAGCTC TCCAAGGCTA TCGAGTTATT GATATTGCCT GTGGCAGTGG AGATGCACAG 
ACACTTTGCC TTACTGATGA TGATACTGTA TGGTCCTGGG GTGATGGGGA CTATGGAAAA 
CTTGGCAGAG GAGGCAGTGA TGGCTGTAAA GTGCCAATGA AGATTGATTC TCTCACAGGT 
CTTGGAGTGG TTAAAGTAGA GTGTGGATCA CAATTTTCTG TTGCACTTAC CAAGTCTGGA 
GCAGTTTATA CTTGGGGTAA AGGCGATTAT CACAGGTTGG GTCATGGATC AGACGACCAT 
GTTAGACGGC CTCGGCAAGT GCAAGGCCTA CAAGGAAAGA AAGTAATAGC AATTGCTACT 
GGTTCTTTAC ACTGTGTGTG CTGCACTGAA GACGGTGAAG TTTATACTTG GGGTGACAAT 
GATGAAGGGC AACTTGGGGA TGGAACAACT AATGCAATCC AGAGACCTCG GTTGGTGGCA 
GCACTTCAGG GTAAAAAGAT AAATAGGGTG GCTTGTGGTT CAGCACACAC CTTAGCGTGG 
TCCACGAGTA AACCTGCCAA TGCTGGGAAG CTTCCAACAC AGGTTCCTAT GGAATACAAC 
CATTTACAGG AATTTCTCAT AATTCCTTTA CGAAACAGAC TTTTGTTATT ACATCATATC 
TCAGAACTCT TTTGTCCATG CATTCCCATG TTTGACCTTG AGGGGCGTCT TGATGAAACT 
GGCCAAGGAC CTTCAGTGGG ATTTGACACA TTACGAGGAA TTCTGATATC TCAGGGAAAG 
GAAGCAGCTT TCCGAAAAGT TGTACAAGCA ACTATGGTGA GAGATCGTCA ACATGGTCCT 
GTAGTTGAAT TAAACAGAAT ACAGGTAAAA CGATCAAGAA GTAAGGGTGG TTTGGCTGGT 
CCTGATGGTA CTAAATCTGT TTTTGGCCAA ATGTGTGCTA AAATGATTTC ATTTAGTCCA 
GACAGTCTTC TTCTACCTCA TCGAGTATGG AAAGTCAAGT TTGTTGGCGA GTCTGTGGAT 
GATTGTGGAG GTGGTTATAG TGAATCAATA GCAGAAATGT GTGAAGAACT TCAGAATGGA 
CTTACTCCTC TCCTAATTGT AACTCCAAAT GGCAGGGATG AATCTGGAGC CAATAGAGAT 
TGCTTCTTAT TGAACCCAGC TGCCAGATCT TCTTTGCATA CCAATATGTT TCGTTTCTTG 
GGTGTATTGC TAGGCATTGC CATTCGAACT GGGAGTCCAT TAAGCCTCAA CCTTGCAGAA 
CCGGTTTGGA AGCAACTGGC AGGAATGAAC CTTACAATTG CTGATCTCAG TGAGGTTGAT 
AAGGATTTTA TTCCTGGCCT GATGTACATT CGGGACAATG AAGCTACTTC TGAGGAATTT 
GAAGCAATGA GCCTTCCCTT CACAGTGCCA AGTGCAAGTG GGCAGGATAT TCAATTGAGC 
TCTAAATATA CCCACATCAC ATTGGATAAC CGTGCTGAGT ATGTTAGATT GGCAATAAAT 
TACAGACTCC ATGAATTTGA TGAACAAGTT GCTGCAGTTC GGGAAGGAAT GGCTCGAGTT 
GTCCCTGTTC CTCTTCTTTC ACTCTTTACT GGATATGAAC TGGAAACTAT GGTGTGTGGA 
AGCCCCGATA TTCCACTTCA TCTTCTAAAA TCTGTAGCCA CATATAAAGG AATTGAACCT 
ACTGCTTCTT TGATCCAGTG GTTCTGGGAA GTGATGGAAT CTTTCTCTAA TACAGAGCGC 
TCTCTCTTTC TTCGTTTTGT GTGGGGCAGA ACACGACTTC CTAGAACAAT TGCTGATTTC 
AGAGGCAGAG ATTTTGTTAT TCAGGTTTTG GATAAGTACA ATCCACCTGA CCACTTCCTT 
CCAGAGTCAT ATACATGCTT CTTTTTGCTG AAGTTACCCA GGTATTCCTG TAAACAAGTA 
CTAGAGGAAA AACTGAAATA TGCCATTCAT TTTTGCAAGT CTATAGACAC TGATGATTAT 
GCCCGGATAG CACTCACAGG AGAACCAGCT GCTGATGACA GCAGTGATGA TTCAGATAAT 
GAAGATGCAG ATTCATTTGC ATCGGACTCT ACTCAAGATT ATTTAACAGG ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC2 ( XM_031959089.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031814949.1 Sarcophilus harrisii HECT and RLD domain containing E3 ubiquitin protein ligase 2 (HERC2), transcript variant X2, mRNA.

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CloneID	OSa229206
Clone ID Related Accession (Same CDS sequence)	XM_031959089.1
Accession Version	XM_031959089.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 14502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	E3 ubiquitin-protein ligase HERC2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045428.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTCTG AATCCTTCTG TTTAGCTGCC CAGGCTAGAT TTGACTCCAA GTGGTTAAAA 
ACAGATTTAC AGCTTGCTTT TACAAGAGAT GGACTCTGTG GCCTATGGAA TGAAATGGTC 
AAAGATGGAG AAATTGTATA TCCTGGGACA GATGTAATAC AGAATGGAGA ACTACCTCCT 
CGAAAAGATG ACACCATGGA ATGTCAGAGT GGAACAAAGA AAGAAGATCA AAATGATAAA 
GAGAAGAAAG ATGAAGAGGA AATTCCAGTC CCTATGTATA AGGCCAAATC ACTTGTGGAG 
AGTTGGGTAT GGGGCAAACA ACCAGATGTG AACGAGTTGA AAGAGTGCCT TTATGTGCTA 
GTGAAAGAAC AGCAAACTCT TGCTACACGG ATGGCTACAA CAACTCTTTC AGCAGTAAGG 
CTAAAGCAGA GGCTGGTTAT ACTGGAACGA TATTTCATTG CACTGAATAG AACTGTTCTT 
CAGGAAAATG TCAAAGTTAA GTGGAAAAGC AACAGTATTC CTTTACCTAC TGTCGACAAA 
AAAAGTTCTA GACCTATAGG AAAAGGTGTA GAAGGACTTG CAAGAGTTGG ATCCAGAGCA 
GCCCTTTCAT TTGCTTTTGC ATTCCTACGC AGAGCTTGGC GGTCAGGGGA AGATGCCGAT 
CTATGCAGTG AATTGTTACA GGAGTCTCTT GATGCCTTGA GAGCCCTACC AGAAGCATCT 
CTTTTTGATG AAAGCACAGT GTCATCTGTT TGGTTGGAAG TTGTGGAGAG AGCAACTAAA 
TTCCTAAGTG ATGTTCATGG AACTGCAAGT ACCAAAGGAC CAGGAAACAT TCCATTACAA 
GACCAGCACT TAGCACTTGC CATATTGTTA GAGTTGGCTG TGCAGAGAGG CACTCTAAGC 
CAAATGTTAT CTGCCATTAT GTTACTACTT CAATTATGGG ACAATGGGAC AAGGGAAACA 
GATAATGAAC GATCTGCCCA AGGAACTAGT GCACCACTTC TCCCTTTATT ACAAAGATTT 
CAGAGTATAA TCTGCAATAA AGACATACTT AATACTGAAG ATGAGATTCA GATGCTGTCT 
GGTCTTCTGA GTCCCAATGA AAGTTTTTTG AGGTACCTTA CTTTACCACA AGACAATGAA 
TTAGCCATTG ATTTGCGTCA AACTGCAGTA GTGATTATGG CGCATTTAGA TCGTCTGGCC 
ACTCCCTGTA TGCCTCCTCA GTGTAGTTCT CCAACATCTC ATAAGGGATC TTTACAAGAA 
GTCATAGGTT GGGGCTTAAT AGGATGGAAA TACTATGCTA ATGTGATTGG CCCAATTCAA 
TGTGAAGGAC TTGCAAATCT TGGGATCACA CAGATTGCCT GTGCAGAAAA GCGTTTCCTG 
ATTTTGTCTC GGAATGGGCG AGTTTACATG CAGGCTTACA ACAGTGACAC ATTGGGACCC 
CAACTAGTTC AAGGTCTTGC CTCTAGAAAT ATTGTGAAAA TTGCAGCACA TTCAGATGGT 
CATCATTATC TGGCTTTGGC AGCCACTGGA GAGGTCTTTT CTTGGGGCTG TGGAGATGGT 
GGACGGTTAG GCCATGGTGA TACAGTGCCT CTAGAAGAAC CTAAGATGAT ATCTGCTCTC 
TCAGGAAAGC AAGCTGGGAA ACATGTAGTT CATATCGCAT GTGGAAGTAC TTATAGTGCA 
GCTATTACAG CTGAAGGAGA GTTATATACT TGGGGTCGAG GAAACTATGG CAGGCTGGGA 
CATGGCTCAA GTGAAGATCA GACAATTCCA ATGCTAGTAA CTGGACTTAA AGGACTGAAG 
GTTATTGATG TCTCTTGTGG GAGTGGGGAT GCTCAGACTC TTGCAGTGAC TGAGAATGGT 
CAAGTCTGGT CTTGGGGCGA TGGTGATTAT GGAAAACTAG GAAGAGGTGG TAGTGATGGT 
TGTAAAACAC CAAAACTGAT TGAAAAACTT CAAGACTTAG ATGTTGTTAA AGTACGTTGT 
GGAAGCCAAT TTTCCGTTGC TTTAACAAAA GATGGTCAAG TTTACTCGTG GGGAAAAGGT 
GACAATCAGA GACTTGGGCA TGGAACAGAG GAGCATGTTC GATATCCGAA ACTGTTAGAA 
GGCTTACAAG GTAAAAAAGT CGTTGATGTG GCTGCTGGTT CTACACACTG CTTAGCACTT 
ACTGAGGATA GCGAAGTTCA TAGCTGGGGG AGTAATGATC AGTGTCAGCA CTTTGACACC 
CTTCGGATTA CCAAACCTGA ACCTACTGCA TTGCCAGGAT TAGATACAAA ACATATAGTT 
GGAATTGCTT GTGGGCCCGC TCAGAGTTTT GCTTGGTCTT CTTGTTCTGA GTGGTCCATT 
GGCCTACGTG TACCTTTTGT GGTGGATGTT TGTCCCATGA CATTTGAACA GTTGGATCTC 
TTACTACGAC AAGTAAGTGA GGGAATGGAT GGCACCTCTG ACTGGCCACC ACCACAGGAG 
AAAGAATGTA TGGCAGTGGC AACATTGAAC CTTCTGAGAC TTCAGTTGCA TGCTGCTATT 
AGTCACCAGG TTGATCCGGA ATGTCTTGGA TTGGGTCTGG GAAGTGTACT CTTGAATAGC 
TTGAAGCAGA CAGTTGTAAC ACTAGCAAGT AATGCTGGAG TTCTTAATAC AGTGCAATCA 
GCTGCTCAAG CAGTACTGCA GAGTGGATGG TCTGTGCTGC TTCCAACTGC AGAGGAACGA 
GCAAGGGCTC TTTCAGCTCT CTTACCCAGT GCAGTTTCAG GAAATGACAT GAATGTAAGT 
CCAGGACGTC GTTTTATGAT TGATCTTCTT GTAGGCAGTT TGATGGCTGA TGGAGGATTA 
GAATCAGCAT TAAATGCTGC CATCACTGCA GAAATCCAGG ATATTGAAGC AAAAAAAGAA 
GCTCAAAAGG AAAAAGAGAT TGATGAGCAG GAAGCAAATG CTTCAACACT TCATCGGAGT 
AGGACACCAT TAGATAAAGA CCTCATTAAT ACTGGAATTT ATGAATCTGC CGGAAAACAG 
AGTTTGCCTC TGGTTCAATT AATACAACAG CTTCTTAGAA ATATTGCTTC CCAGACAATA 
GCCAAATTGA AAGATGTTGC TCGTCGAATT TTGTCCTGTA TAGAACATGA TCAATATAGT 
CAAGACAGAT CTGCCTCCCT GGATTTGTTG CTTCGTTTTC AGCGTCTGCT GGTCAGTAAA 
CTCTATCCAG GAGACAGTGG TGGTCAAGTT TCAAATTCCT TTAGTCCTGA GCTATTAGGA 
GTTGGTTCTT TGCTGAAAAA ATATACAGCT CTTTTGTGCA CACACATTGG AGATATATTG 
CCTGTGGCAA CTAGTATTGC ATCTTCCAGT TATCGGCATT TTGCAGAAGT ATCTCGTGTT 
GTGGATGGTG ATCTAACAGG TGTTCTTCTT CCAGAGTTGA TAGTATCTAT TGTCCTTTTG 
CTCAATAAAA GTCCTGGATT AATGCAGGAA GCTGGGGCAA TTCCTCTCTT AGCTGGTCTA 
CTGGAACATC TAGATAGATT TAACCATTTG GCACCAGGAA AAGAGAGAGA TGATAATGAG 
GATTTAGCCT GGCCTGGAAT AATGGGATCA TTTTTTACAG GTCAGAATAG TAGAAATAAT 
GAGGAAGTGA CGCTCATACG TAAAGCTGAT CTGGAGAATC ACAATAAAGA TGGAGGCTTC 
TGGACTGTGA TTGACGGGAA AGTGTATGAT ATAAAAGACT TTCAGACACA GTCTTTAACA 
GGCAATAGCA TTCTTGCTCA GTTTGCAGGT GATGACCCGG TGGTTGCTTT GGAAGCTGCT 
TTACAGTTTG AGGACACAAG GGATTCAATG CATGCATTTT GTGTTGGCCA GTATATGGAG 
CCTGACCAGG AAGTGATTAC AACGCCAGAC CTTACCAGTC TCTCTTCACC TCTCATAGAT 
CCAGAAAGAA ATCTGGGTCT CCTCCTTGGA CTTCATGCTT CCTATTTAGC AATGAGCACA 
CCACTATCTC CTGTGGAGAT TGAATGTGCC AAATGGCTTA AGTCATCCAT ATTCTCTGGA 
GGATTGCAAA CCAGTCAGAT ACATTATAGT TACAATGAAG AGAAAGATGA AGACCACTGC 
AGTTCTCCTG GAAATACTAC TCCAAACAAA TCCAAACTCT ATTCCCATAG ATTGACTTTG 
AGTGATCATT CCCAACCATT TTTGCAAGCC ATTGCAGATA ATAACATTCA GGATCATACC 
GTGAAGGATT TTTTATGTCA GATAGAAAGG TATTGCAAGC AATGCCATTT GACCACACCG 
ATAACATTTC CTCCTGAACA TCCTGTGGAA GAAGTGGGAC GTTTACTCTT ATGTTGTCTT 
CTAAAGCATG AAGATTTAGG TCATGTGGCC TTATCTTTAG TTCACCCAGT TACACTTGGT 
GTTGACCAAG TCAAACACAG AACGTTGCCT AAGTCTGTAG TGGATGTTTG TAGAGTCGTC 
TACCAGGCAA AATGCTCACT AATAAAGACA CATCAGGAAC AGGGACGTTC TTATAAGGAA 
GTGTGTGCTC CTGTTATTGA ACGTTTGCGA TTTCTTTTTA ATGAATTGCG TCCTGCAGTC 
TGCAATGACC TCTCAATAAT GTCCAAACTT AAACTTTTAA GCTCTTTGCC TCGTTGGAGA 
AGAATAGCTC AGAAGATGAT ACGAGAGAGA AGGAAATTAA AAAGAGTACC CAAAAAGTCA 
GAACCTACTG ATGTGGAAGA ATCTAAGATT GGCAATGAAG AGGGTGACTT AGAAGAAGCC 
TGTATTCTTC CTCATAGTCC TGTATCTGTA GATAAAAGAC CTGTTCCAGT TAAATCACCT 
AAGGATAAAT GGCAGCCACT TCTGAGTACA GTTACAGGTG TTCACAAATA CAAATGGCTT 
AAACAGAATG TTCAAGGCCT TTATCCTCAG TCTGCACTGC TTAGCACAAT TGTTGAATTT 
GCGCTTAAAG AAGAGCCAGT TGATGTGGAA AAGATGAGAA AGTGCTTACT CAAACAGTTG 
GAGAGAGCAG AAGTTCGTTT GGAAGGAATA GACACGATTT TAAAATTGGC AAATAAAAAT 
TTCTTGCTTT CGTCTGTGCA ATATGCTATG TTCTGTGGAT GGCAAAGACT TATCCCAGAA 
GGAACCAATA TAGGGGAACC ACTTACAGAT TGTTTAAAGG ATGTTGATCT GATTCCACCA 
TTTAATCGAA TGCTACTAGA AGTAACTTTT GGAAAGTTGT ATGGTTGGGC CATTCAGAAT 
ATTCGAAATG TTTTGTTGGA TGCAAATGCC AGATTTAAAG AGCTAGGTAT CCAGCCTGTT 
CCTCTGCAAA CTATTACTAA TGAGAATCCA GCAGGGCCAA GCCTTGGAAC CATCCCACAA 
GCACGTTTTC TCCTGGTTAT GCTCAACATG CTTACCCTTC AATATGGTGC AAACAACTTG 
AATCTTCTTT TGAACTCTGG CATGCTAGCA TTGACTCAGA CAACATTGCG ATTAATAGGT 
CCTAGTTCTG ATAATATCGA GGAAGAAATG AATGCCTCTG CACGAGGAGC TTCAGCTACT 
GTTTTGGAAG AATCCAGAAA AGAAACCACT CCAGTACAAC TTCCTGTTTC AGGGCCAGAA 
CTGGCAGCAA TGATGAAAAT TGGTACCAGG GTCATGAGGG GAGTGGACTG GAAATGGGGA 
GATCAGGATG GTCCACCTCC AGGTTTAGGT CGTGTGATTG GTGAATTGGG AGAGGATGGA 
TGGATAAGGG TTCAGTGGGA TACAGGCAGT ACTAATTCAT ACCGAATGGG AAAGGAAGGA 
AAGTATGATC TTAAATTGGC TGAGCCTCCA CCTGCAGCTC AGCCCTCAAC AGAGGATTCG 
GACACTGAGG ATGATTCTGA AAGTGAACAA ATGGAAAGAA ACTTCCACCC TACTTCTATT 
ATGCTTACAA GTACTGTTAA TTTACTCCAA ACTCTCTGTC TCTGTGCTGG AATTCATGCA 
GAAATCATGC AAAATGAAGC TACAAGAACT CTGTGTGGTC TACTTAGAAT GTTAGTGGAA 
AGTGGAACAG TAGATAAAAC ATCTTCTTTA CCAAATAAGA TGGTTTATAA AGAGCAGCAC 
AGAAGTTGGT GTACACTTGG ATTTATTCGA AGTATAGCAC TTATGCCTCA AATGTGTAGC 
ACCCTTAGCA CATCTCAGTG GATCACATTA CTAATGAAAA TTGTGGAAGG ACACGAGTCA 
TTTACAGCAG CTTCGCTTCA GAGACAGATC TTAGCAGTAC ATTTACTACA AGCTGTACTT 
CCATCTTGGG ACAAAACAGA AAGATCAAGA GATATGAAGT TTCTTGTGGA AAAACTTTTT 
GGCTTCTTGG GCAGTCTACT TAGTACATGC TCTTCAGACA TACCTTTGCT CAGAGAATCA 
ACTCTGAGAA GGAGGAAGTC CAGACCCCAG GCATCTTTGA CTGCAACCCA TAGTAGTACT 
TTGGCAGAGG AGATAGTGGC CCTGCTAAGG ACTCTACATT CACTGAGCCA GTGGAATGGA 
CTCATAAACA AGTACATCAA CTCTCAGCTA AACTCCATCA CCCACATCTT TGCAGGAAAA 
CAGTCTGAAG GGACTGTGTT AGAAGATTAC TTTCCTGATT CTGAGAACCC TGAAGTTGAT 
GGCCTAATGG CAGTGCTAGC TGTAATTGGT GGAATAGATA GTCGATTGAG GCTGGGTGGC 
CAAGTGGTAC ATGATGAATT TGGAGAAGGC ACAGTGACAC GCATCACTCC TAAAGGCAAA 
ATCACAGTCC AGTTCTGTGA TATGCGAATA TGTAGAATTT GTCCATTGAG TCAGCTAAAA 
CCGCTTCCAG TTATGGCATT TAATGTGAAC AACTTACCAT TCACGGAATC TATGCTCTCT 
GTCTGGGCTC AATTGGTCAA CTTGGCAGGG AGCAAATTAG AAAAACACAG AATTAAGAAA 
TCTACTAATC AAGCCCTCAC AGGACAAGTA GACTTGGATT TGCTAAGATG CCAACAGTTG 
AAATTATACA TATTGAAAGC AGGTCGAGCA TTGCTTTCCC ATCAGGATAA ACTGAGGCAG 
ATCTTATCTC AACCAGCTTT ACAAGAGAAT GGAGTTCTCT CTACAGATGA TGGTACAGCA 
TCCCCTGATA TTGGAGATAT GTCTCCTGAA GGACCTCAAC CTCCAATGAT CCTTTTACAG 
CAATTGTTGA CATCTGCCAC ACAGCCATCA CCTGTAAAAG CAATATTTGA CAAACAGGAA 
CTTGAGGCTG CTGCTTTGGC TGTATGCCAG TGTCTAGCTG TGGAGTCAAC TCATCCATCA 
ACTCCAGTGT TTGAAGAATG TAGCTCTAGT GAGGCCACAA CACCTGTCAC AGTCCAGCAT 
ATTCGATCTG TAAAAGTGAA AAAGCGAAAG CAGTCTCCTC TCCCTGCTTT GCCTATTGTA 
GTACAGCTCA TGGAAATGGG ATTTCCGAGG AAAAACATTG AATTTGCATT GAAGTCTCTT 
ACTGGGAGTG CTTCAGGGTT ACCAGGTGTT GAAGCCTTGG TTGGTTGGTT GCTGGATCAT 
CCTGATGTCC AAATTACTGA TCTCTCAGAT GCTGATACAG TCTCTGATGA ATACTCTGAT 
GATGAAATAG TGGAAGATGT GGAAGAGACT GAAGCATCTT ACCCTGTGTC TAGTTGTGCT 
GTTGTAACGG AGAGTCACAC TTATAAAAAA CGAGCTGATT TCTTAAGTAA TGATGACTAT 
GCAGTATATG TTAGAGAAAA CATTCAGGTA GGAATGATGG TTAGGTGCTG CAGAACGTAT 
GAAGAAGTTT GTGAAGGAGA TGTAGGTAAA GTGATTAAAT TGGACAGAGA TGGATTACAC 
GACCTCAATG TGCAGTGTGA TTGGCAACAA AAGGGGAGTA CCTATTGGGT TAGGTATATC 
CATGTTGAAC TTATAGGTTT CCCACCACAG AGTTCTCCTT CCCATATCAA GATTGGTGAT 
AAAGTTAGGG TGAAAACATC AGTAACCACA CCAAAATATA AATGGGGATC AGTTACTCAT 
AGAAGTGTGG GAGTTGTGAA AGCTTTTAGT GCCAATGGAA AAGATGTTAT TGTGGATTTT 
CCTCAGCAGT CTCATTGGAC AGGATTATTG TCAGAAATGG AATTGGTGCC TAGTATTCAT 
CCTGGAGTTA CGTGTGATGG CTGCCAAATG TTTCCTATCA ATGGTCCTCG TTTCAAATGC 
AGGAGTTGTG ATGATTTTGA CTTTTGTGAG ACCTGTTTTA AAACCAAGAA GCATAATACC 
AGACATAGTT TTGGCAGAAT AAATGAACCA GGTCAGTCTC TTGTATTTTG TGGGCGTTCT 
GGGAAACAGC TAAAACGTTG CCACAGTAGT CAGCCTGGAA TGCTCTTGGA TAATTGGTCA 
AGAATTGTTA AGAATTTAAA TGTGTCCTCA TCAGTAAATC AGGCTTCTCG CTTGATTGAT 
GGCAGTGAAC CATGTTGGCA GTCATCTGGA TCACAAGGAA AGCATTGGAT TCGACTGGAG 
ATTTTCCCCG ATGTTCTTGT TCATAGACTG AAAATGATAG TAGATCCTGC TGACAGTAGC 
TACATGCCTT CACTAGTTGT AGTTTCAGGT GGGAATTCCC TAAATAATTT AATTGAACTG 
AAGACAATCA ATATTAACCC AGCTGATACC ACAGTGCCTC TTCTAAATGA CTGCACAGAG 
TATCACAGGT ATATTGAGGT TGCCATAAAG CAATGCAGAA GCTCAGGAAT AGATTGTAAA 
ATTCATGGTC TTATTATACT GGGACGAATT CGGGCAGAAG ATGAAGATTT GGCTGCTGTT 
CCTTTCTTAG CGTCAGATAA TGAAGAAGAA GATGATGAAA AAGCTAACAC TGGGAGTCTT 
ATTAGGAAGA AGGCTTCTGG ATTGGAATCA GCAGCCACAA TAAGGACCAA AGTTTTTGTC 
TGGGGGCTGA ATGACAAGGA TCAATTAGGA GGATTAAAGG GTTCTAAGAT AAAAGTTCCT 
TCATTCTCTG AAACCCTTTC TGCTTTGAAT GTAGTTCAAG TGGCAGGTGG ATCTAAAAGT 
TTGTTTGCAG TGACTGTGGA GGGTAAAGTG TATGCTTGTG GAGAAGCTAC TAATGGACGA 
CTGGGTTTAG GTATTTCTAG TGGAACTGTA CCCATTCCGC GGCAGATTAC AGCTCTCAGC 
AATTATGTGG TAAAGAAAGT TGCTGTTCAC TCAGGTGGCC GGCATGCTAT GGCTTTAACT 
GTTGATGGAA AGATATTTTC ATGGGGTGAA GGTGATGATG GAAAACTAGG ACACTTCAGC 
AGAATGAACT GTGATAAACC AAGACTGATA GAGGCATTGA AAACCAAACG TATTCGTGAT 
ATAGCTTGTG GTAGTTCTCA CAGTGCTGCC ATTACATCAA GTGGTGAATT GTATACATGG 
GGCCTTGGAG AATATGGCCG GTTGGGACAT GGGGATAATA CTACACAGCT GAAACCCAAA 
ATGGTGAAAG TTCTTCTTGG TCATCGAGTT ATTCAAGTTG CTTGTGGAAG TAGAGATGCC 
CAGACTCTGG CTTTGACAGA TGAAGGTTTG GTCTTTTCCT GGGGAGATGG TGACTTTGGG 
AAACTGGGCC GGGGAGGAAG TGAAGGATGT AACATTCCTC AGAACATTGA GAGACTTAAT 
GGACAAGGAG TTTGCCAGAT TGAGTGTGGG GCTCAATTTT CCTTGGCACT TACAAAGTCA 
GGAGTAGTAT GGACTTGGGG TAAAGGAGAT TACTTTAGGC TTGGTCATGG CACTGATGTT 
CATGTGAGAA AACCACAAGT AGTAGAAGGA CTACGAGGAA AGAAGATAGT TCATGTGGCA 
GTAGGCGCTC TTCACTGCTT AGCTGTCACT GATACAGGTC AAGTGTATGC TTGGGGTGAC 
AATGATCATG GACAACAAGG CAATGGGACT ACTACTGTCA ACAGAAAGCC TACCCTTGTA 
CAAGGCTTAG AAGGACAAAA AATCACTCGG GTAGCTTGTG GCTCATCTCA TAGTGTGGCG 
TGGACAACAG TGGATGTAGC AACCCCATCT GTTCATGAAC CTGTCCTCTT TCAAACTGCT 
AGGGACCCTT TAGGCGCTTC TTACTTAGGT GTTCCTTCCG ATGCTGATTC TTCTGCTGCC 
AGTAACAAAA TCAGTGGTGT AAATAGTTCC AAGCCTAACC GCCCTTCCCT TGCTAAGATT 
CTCTTGTCTT TGGATGGAAA CCTTGCCAAA CAGCAAGCAT TATCTCATAT ACTTACAGCA 
TTGCAAATTA TGTATGCGAG AGATGCAGTA GTTGGAGCAT TAATGCCTGC TAGTATGATC 
GCACCAGTGG AATGCTCATC TTCTTCATCA CCTGCACCCC CCTCAGATGT GTCTTCAGCA 
GGAAGTCCCA TGAATGGTGA AGAGTCTGTG TTAGCTGTGG ATGTAGAAGA TAGACTAAGC 
CCAAATCCAT GGCAAGACAA GAGAGGGGAG GTAGTTTCTT CAGAAGATGC TGTGACTCCA 
TCAGCAGTGA CTCCCTCAGC TTCCTCTGCC TCTTCTCGAC CATTTATTCC AGTAACTGAT 
GATCCTGGAG CTGCCAGTAT CATTGCAGAA ACCATGACTA AAACCAAAGA AGATGTTGAA 
AGCCAAAATA AAGTATCAGG TCCGGAACCT CAATATTTGG ATGAATTTAC TAGTCTCTTA 
GTGCCAGATG ATACCCGTGT CATGGTTGAC TTGCTCAAGC TGTCTGTTTC TAATCGAGCT 
GGTGAAAAGG GAAAAGATGT TCTTTCAGCT GTGCTTTCTG GTATGGGCAC TGCTTATCCA 
CAGGTAGCTG ATATGTTGTT GGAGCTCTGT GTTACAGAGT TAGAAGATGT TGCCACAGAC 
TCTCAGAGTG GTCGACTCTC TTCCCAACCT GTTGTAGTAG AAAGTAGTCA TCCGTATACT 
GATGATACCT CTACCAGTGG TACTGTCAAA ATACCAGGTG CTGAAGGACT CAGAGTAGAG 
TTTGATCGAC AATGTTCTAC AGAACGGCGT CATGACCCTC TCACAATCAT GGATGGTGTC 
AATAGGATAG TTTCAGTTCG ATCAGGTCGA GAATGGTCTG ATTGGTCCAG TGAACTTCGA 
ATTCCTGGAG ATGAATTAAA GTGGAAGTTC ATCAGTGATG GATCAGTGAA TGGATGGGGT 
TGGCGATTCA CTGTCTATCC TATTATGCCA GCTGCAGGCC CTAAAGATCT TCTTTCAGAT 
CGGTGTATTC TTTCCTGTCC CTCAATGGAC TTGGTTACAT GCTTGTTGGA CTTTAGATTA 
AATTTAGCTT CCAATAGAAG TATTGTTCCT CGTTTGGCAG CATCACTTGC AGCATGTGCT 
CAATTGAGTG CTCTAGCTGC TGGTCACAGA ATGTGGGCCC TTCAAAGACT GAGGAAACTG 
CTTACAACTG AATTTGGACA ATCAATCAAT ATAAATAGGA TACTTGGAGA TAATGATGGA 
GAAACAAGAA CCATGAGTTT TACCGGTAGT GCTTTAGCAG CTTTGGTAAA AGGTCTTCCA 
GAAGCTTTGC AAAGACAGTT TGAATATGAA GATCCGATTG TTAGAGGAGG CAAACAACTG 
CTCCATAGCC CTTTCTTTAA GGTGCTGGTA GCTCTTGCTT GTGACCTAGA ACTGGATACT 
CTTCCTTGCT GTGCTGAGAC ACACAAATGG GCTTGGTTTA GAAGATACTG TATGGCCTCT 
AGAGTTTCAG TAGCCTTGGA CAAACGAACA CCATTACCTC GCCTTTTCCT TGATGAGGTG 
GCAAAGAAAA TCAGGGAGTT AATGGCCGAC AGTGAAAATC TGGATGTTCT GCATGAGAGC 
CATGACATTT TTAAGAGAGA ACAAGATGAA CAACTCGTCC AGTGGATGAA CAGGAGACCT 
GATGACTGGA CTCTTTCTGC TGGAGGCAGT GGAACAATTT ATGGTTGGGG TCACAATCAT 
CGAGGACAGC TTGGCGGTAT TGAGGGAGCA AAAGTCAAAG TTCCAACACC TTGTGAAGCT 
CTTGCAGCTC TGCGGCCCAT ACAGTTAATT GGAGGGGAAC AGACTCTCTT TGCGGTAACT 
GCTGATGGGA AGCTTTATGC TACAGGGTAT GGTGCAGGTG GCAGATTGGG AATTGGAGGA 
ACTGAATCAG TTTCTACTCC AACATTGCTT GAATCTATTC AGCATGTATT TATAAAAAAA 
GTTGCTGTGA ACTCTGGAGG AAAACACTGC TTAGCACTCT CATCAGAAGG AGAAGTTTAT 
TCTTGGGGTG AGGCAGAAGA TGGAAAATTG GGGCATGGAA ACAGAAGCCC CTGTGATCGC 
CCACGTGTAA TTGAATCTTT GCGAGGAATA GAAGTTGTTG ATATTGCTGC AGGTGGAGCC 
CACAGTGCAT GTATTACAGC AGCTGGTGAC CTCTATACGT GGGGTAAAGG AAGATATGGT 
CGCCTAGGTC ATGGGGATAG TGAAGATCAG TTAAAACCAA AACTGGTGGA AGCTCTCCAA 
GGCTATCGAG TTATTGATAT TGCCTGTGGC AGTGGAGATG CACAGACACT TTGCCTTACT 
GATGATGATA CTGTATGGTC CTGGGGTGAT GGGGACTATG GAAAACTTGG CAGAGGAGGC 
AGTGATGGCT GTAAAGTGCC AATGAAGATT GATTCTCTCA CAGGTCTTGG AGTGGTTAAA 
GTAGAGTGTG GATCACAATT TTCTGTTGCA CTTACCAAGT CTGGAGCAGT TTATACTTGG 
GGTAAAGGCG ATTATCACAG GTTGGGTCAT GGATCAGACG ACCATGTTAG ACGGCCTCGG 
CAAGTGCAAG GCCTACAAGG AAAGAAAGTA ATAGCAATTG CTACTGGTTC TTTACACTGT 
GTGTGCTGCA CTGAAGACGG TGAAGTTTAT ACTTGGGGTG ACAATGATGA AGGGCAACTT 
GGGGATGGAA CAACTAATGC AATCCAGAGA CCTCGGTTGG TGGCAGCACT TCAGGGTAAA 
AAGATAAATA GGGTGGCTTG TGGTTCAGCA CACACCTTAG CGTGGTCCAC GAGTAAACCT 
GCCAATGCTG GGAAGCTTCC AACACAGGTT CCTATGGAAT ACAACCATTT ACAGGAATTT 
CTCATAATTC CTTTACGAAA CAGACTTTTG TTATTACATC ATATCTCAGA ACTCTTTTGT 
CCATGCATTC CCATGTTTGA CCTTGAGGGG CGTCTTGATG AAACTGGCCA AGGACCTTCA 
GTGGGATTTG ACACATTACG AGGAATTCTG ATATCTCAGG GAAAGGAAGC AGCTTTCCGA 
AAAGTTGTAC AAGCAACTAT GGTGAGAGAT CGTCAACATG GTCCTGTAGT TGAATTAAAC 
AGAATACAGG TAAAACGATC AAGAAGTAAG GGTGGTTTGG CTGGTCCTGA TGGTACTAAA 
TCTGTTTTTG GCCAAATGTG TGCTAAAATG ATTTCATTTA GTCCAGACAG TCTTCTTCTA 
CCTCATCGAG TATGGAAAGT CAAGTTTGTT GGCGAGTCTG TGGATGATTG TGGAGGTGGT 
TATAGTGAAT CAATAGCAGA AATGTGTGAA GAACTTCAGA ATGGACTTAC TCCTCTCCTA 
ATTGTAACTC CAAATGGCAG GGATGAATCT GGAGCCAATA GAGATTGCTT CTTATTGAAC 
CCAGCTGCCA GATCTTCTTT GCATACCAAT ATGTTTCGTT TCTTGGGTGT ATTGCTAGGC 
ATTGCCATTC GAACTGGGAG TCCATTAAGC CTCAACCTTG CAGAACCGGT TTGGAAGCAA 
CTGGCAGGAA TGAACCTTAC AATTGCTGAT CTCAGTGAGG TTGATAAGGA TTTTATTCCT 
GGCCTGATGT ACATTCGGGA CAATGAAGCT ACTTCTGAGG AATTTGAAGC AATGAGCCTT 
CCCTTCACAG TGCCAAGTGC AAGTGGGCAG GATATTCAAT TGAGCTCTAA ATATACCCAC 
ATCACATTGG ATAACCGTGC TGAGTATGTT AGATTGGCAA TAAATTACAG ACTCCATGAA 
TTTGATGAAC AAGTTGCTGC AGTTCGGGAA GGAATGGCTC GAGTTGTCCC TGTTCCTCTT 
CTTTCACTCT TTACTGGATA TGAACTGGAA ACTATGGTGT GTGGAAGCCC CGATATTCCA 
CTTCATCTTC TAAAATCTGT AGCCACATAT AAAGGAATTG AACCTACTGC TTCTTTGATC 
CAGTGGTTCT GGGAAGTGAT GGAATCTTTC TCTAATACAG AGCGCTCTCT CTTTCTTCGT 
TTTGTGTGGG GCAGAACACG ACTTCCTAGA ACAATTGCTG ATTTCAGAGG CAGAGATTTT 
GTTATTCAGG TTTTGGATAA GTACAATCCA CCTGACCACT TCCTTCCAGA GTCATATACA 
TGCTTCTTTT TGCTGAAGTT ACCCAGGTAT TCCTGTAAAC AAGTACTAGA GGAAAAACTG 
AAATATGCCA TTCATTTTTG CAAGTCTATA GACACTGATG ATTATGCCCG GATAGCACTC 
ACAGGAGAAC CAGCTGCTGA TGACAGCAGT GATGATTCAG ATAATGAAGA TGCAGATTCA 
TTTGCATCGG ACTCTACTCA AGATTATTTA ACAGGACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031814949.1
CDS	258..14759
Translation	MSSESFCLAAQARFDSKWLKTDLQLAFTRDGLCGLWNEMVKDGEIVYPGTDVIQNGELPPRKDDTMECQSGTKKEDQNDKEKKDEEEIPVPMYKAKSLVESWVWGKQPDVNELKECLYVLVKEQQTLATRMATTTLSAVRLKQRLVILERYFIALNRTVLQENVKVKWKSNSIPLPTVDKKSSRPIGKGVEGLARVGSRAALSFAFAFLRRAWRSGEDADLCSELLQESLDALRALPEASLFDESTVSSVWLEVVERATKFLSDVHGTASTKGPGNIPLQDQHLALAILLELAVQRGTLSQMLSAIMLLLQLWDNGTRETDNERSAQGTSAPLLPLLQRFQSIICNKDILNTEDEIQMLSGLLSPNESFLRYLTLPQDNELAIDLRQTAVVIMAHLDRLATPCMPPQCSSPTSHKGSLQEVIGWGLIGWKYYANVIGPIQCEGLANLGITQIACAEKRFLILSRNGRVYMQAYNSDTLGPQLVQGLASRNIVKIAAHSDGHHYLALAATGEVFSWGCGDGGRLGHGDTVPLEEPKMISALSGKQAGKHVVHIACGSTYSAAITAEGELYTWGRGNYGRLGHGSSEDQTIPMLVTGLKGLKVIDVSCGSGDAQTLAVTENGQVWSWGDGDYGKLGRGGSDGCKTPKLIEKLQDLDVVKVRCGSQFSVALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVVDVAAGSTHCLALTEDSEVHSWGSNDQCQHFDTLRITKPEPTALPGLDTKHIVGIACGPAQSFAWSSCSEWSIGLRVPFVVDVCPMTFEQLDLLLRQVSEGMDGTSDWPPPQEKECMAVATLNLLRLQLHAAISHQVDPECLGLGLGSVLLNSLKQTVVTLASNAGVLNTVQSAAQAVLQSGWSVLLPTAEERARALSALLPSAVSGNDMNVSPGRRFMIDLLVGSLMADGGLESALNAAITAEIQDIEAKKEAQKEKEIDEQEANASTLHRSRTPLDKDLINTGIYESAGKQSLPLVQLIQQLLRNIASQTIAKLKDVARRILSCIEHDQYSQDRSASLDLLLRFQRLLVSKLYPGDSGGQVSNSFSPELLGVGSLLKKYTALLCTHIGDILPVATSIASSSYRHFAEVSRVVDGDLTGVLLPELIVSIVLLLNKSPGLMQEAGAIPLLAGLLEHLDRFNHLAPGKERDDNEDLAWPGIMGSFFTGQNSRNNEEVTLIRKADLENHNKDGGFWTVIDGKVYDIKDFQTQSLTGNSILAQFAGDDPVVALEAALQFEDTRDSMHAFCVGQYMEPDQEVITTPDLTSLSSPLIDPERNLGLLLGLHASYLAMSTPLSPVEIECAKWLKSSIFSGGLQTSQIHYSYNEEKDEDHCSSPGNTTPNKSKLYSHRLTLSDHSQPFLQAIADNNIQDHTVKDFLCQIERYCKQCHLTTPITFPPEHPVEEVGRLLLCCLLKHEDLGHVALSLVHPVTLGVDQVKHRTLPKSVVDVCRVVYQAKCSLIKTHQEQGRSYKEVCAPVIERLRFLFNELRPAVCNDLSIMSKLKLLSSLPRWRRIAQKMIRERRKLKRVPKKSEPTDVEESKIGNEEGDLEEACILPHSPVSVDKRPVPVKSPKDKWQPLLSTVTGVHKYKWLKQNVQGLYPQSALLSTIVEFALKEEPVDVEKMRKCLLKQLERAEVRLEGIDTILKLANKNFLLSSVQYAMFCGWQRLIPEGTNIGEPLTDCLKDVDLIPPFNRMLLEVTFGKLYGWAIQNIRNVLLDANARFKELGIQPVPLQTITNENPAGPSLGTIPQARFLLVMLNMLTLQYGANNLNLLLNSGMLALTQTTLRLIGPSSDNIEEEMNASARGASATVLEESRKETTPVQLPVSGPELAAMMKIGTRVMRGVDWKWGDQDGPPPGLGRVIGELGEDGWIRVQWDTGSTNSYRMGKEGKYDLKLAEPPPAAQPSTEDSDTEDDSESEQMERNFHPTSIMLTSTVNLLQTLCLCAGIHAEIMQNEATRTLCGLLRMLVESGTVDKTSSLPNKMVYKEQHRSWCTLGFIRSIALMPQMCSTLSTSQWITLLMKIVEGHESFTAASLQRQILAVHLLQAVLPSWDKTERSRDMKFLVEKLFGFLGSLLSTCSSDIPLLRESTLRRRKSRPQASLTATHSSTLAEEIVALLRTLHSLSQWNGLINKYINSQLNSITHIFAGKQSEGTVLEDYFPDSENPEVDGLMAVLAVIGGIDSRLRLGGQVVHDEFGEGTVTRITPKGKITVQFCDMRICRICPLSQLKPLPVMAFNVNNLPFTESMLSVWAQLVNLAGSKLEKHRIKKSTNQALTGQVDLDLLRCQQLKLYILKAGRALLSHQDKLRQILSQPALQENGVLSTDDGTASPDIGDMSPEGPQPPMILLQQLLTSATQPSPVKAIFDKQELEAAALAVCQCLAVESTHPSTPVFEECSSSEATTPVTVQHIRSVKVKKRKQSPLPALPIVVQLMEMGFPRKNIEFALKSLTGSASGLPGVEALVGWLLDHPDVQITDLSDADTVSDEYSDDEIVEDVEETEASYPVSSCAVVTESHTYKKRADFLSNDDYAVYVRENIQVGMMVRCCRTYEEVCEGDVGKVIKLDRDGLHDLNVQCDWQQKGSTYWVRYIHVELIGFPPQSSPSHIKIGDKVRVKTSVTTPKYKWGSVTHRSVGVVKAFSANGKDVIVDFPQQSHWTGLLSEMELVPSIHPGVTCDGCQMFPINGPRFKCRSCDDFDFCETCFKTKKHNTRHSFGRINEPGQSLVFCGRSGKQLKRCHSSQPGMLLDNWSRIVKNLNVSSSVNQASRLIDGSEPCWQSSGSQGKHWIRLEIFPDVLVHRLKMIVDPADSSYMPSLVVVSGGNSLNNLIELKTININPADTTVPLLNDCTEYHRYIEVAIKQCRSSGIDCKIHGLIILGRIRAEDEDLAAVPFLASDNEEEDDEKANTGSLIRKKASGLESAATIRTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALNVVQVAGGSKSLFAVTVEGKVYACGEATNGRLGLGISSGTVPIPRQITALSNYVVKKVAVHSGGRHAMALTVDGKIFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAAITSSGELYTWGLGEYGRLGHGDNTTQLKPKMVKVLLGHRVIQVACGSRDAQTLALTDEGLVFSWGDGDFGKLGRGGSEGCNIPQNIERLNGQGVCQIECGAQFSLALTKSGVVWTWGKGDYFRLGHGTDVHVRKPQVVEGLRGKKIVHVAVGALHCLAVTDTGQVYAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSVAWTTVDVATPSVHEPVLFQTARDPLGASYLGVPSDADSSAASNKISGVNSSKPNRPSLAKILLSLDGNLAKQQALSHILTALQIMYARDAVVGALMPASMIAPVECSSSSSPAPPSDVSSAGSPMNGEESVLAVDVEDRLSPNPWQDKRGEVVSSEDAVTPSAVTPSASSASSRPFIPVTDDPGAASIIAETMTKTKEDVESQNKVSGPEPQYLDEFTSLLVPDDTRVMVDLLKLSVSNRAGEKGKDVLSAVLSGMGTAYPQVADMLLELCVTELEDVATDSQSGRLSSQPVVVESSHPYTDDTSTSGTVKIPGAEGLRVEFDRQCSTERRHDPLTIMDGVNRIVSVRSGREWSDWSSELRIPGDELKWKFISDGSVNGWGWRFTVYPIMPAAGPKDLLSDRCILSCPSMDLVTCLLDFRLNLASNRSIVPRLAASLAACAQLSALAAGHRMWALQRLRKLLTTEFGQSININRILGDNDGETRTMSFTGSALAALVKGLPEALQRQFEYEDPIVRGGKQLLHSPFFKVLVALACDLELDTLPCCAETHKWAWFRRYCMASRVSVALDKRTPLPRLFLDEVAKKIRELMADSENLDVLHESHDIFKREQDEQLVQWMNRRPDDWTLSAGGSGTIYGWGHNHRGQLGGIEGAKVKVPTPCEALAALRPIQLIGGEQTLFAVTADGKLYATGYGAGGRLGIGGTESVSTPTLLESIQHVFIKKVAVNSGGKHCLALSSEGEVYSWGEAEDGKLGHGNRSPCDRPRVIESLRGIEVVDIAAGGAHSACITAAGDLYTWGKGRYGRLGHGDSEDQLKPKLVEALQGYRVIDIACGSGDAQTLCLTDDDTVWSWGDGDYGKLGRGGSDGCKVPMKIDSLTGLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLGHGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCTEDGEVYTWGDNDEGQLGDGTTNAIQRPRLVAALQGKKINRVACGSAHTLAWSTSKPANAGKLPTQVPMEYNHLQEFLIIPLRNRLLLLHHISELFCPCIPMFDLEGRLDETGQGPSVGFDTLRGILISQGKEAAFRKVVQATMVRDRQHGPVVELNRIQVKRSRSKGGLAGPDGTKSVFGQMCAKMISFSPDSLLLPHRVWKVKFVGESVDDCGGGYSESIAEMCEELQNGLTPLLIVTPNGRDESGANRDCFLLNPAARSSLHTNMFRFLGVLLGIAIRTGSPLSLNLAEPVWKQLAGMNLTIADLSEVDKDFIPGLMYIRDNEATSEEFEAMSLPFTVPSASGQDIQLSSKYTHITLDNRAEYVRLAINYRLHEFDEQVAAVREGMARVVPVPLLSLFTGYELETMVCGSPDIPLHLLKSVATYKGIEPTASLIQWFWEVMESFSNTERSLFLRFVWGRTRLPRTIADFRGRDFVIQVLDKYNPPDHFLPESYTCFFLLKLPRYSCKQVLEEKLKYAIHFCKSIDTDDYARIALTGEPAADDSSDDSDNEDADSFASDSTQDYLTGH

Target ORF information:

RefSeq Version	XM_031959089.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HECT and RLD domain containing E3 ubiquitin protein ligase 2 (HERC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031959089.1

ORF Insert Sequence:

ATGTCTTCTG AATCCTTCTG TTTAGCTGCC CAGGCTAGAT TTGACTCCAA GTGGTTAAAA 
ACAGATTTAC AGCTTGCTTT TACAAGAGAT GGACTCTGTG GCCTATGGAA TGAAATGGTC 
AAAGATGGAG AAATTGTATA TCCTGGGACA GATGTAATAC AGAATGGAGA ACTACCTCCT 
CGAAAAGATG ACACCATGGA ATGTCAGAGT GGAACAAAGA AAGAAGATCA AAATGATAAA 
GAGAAGAAAG ATGAAGAGGA AATTCCAGTC CCTATGTATA AGGCCAAATC ACTTGTGGAG 
AGTTGGGTAT GGGGCAAACA ACCAGATGTG AACGAGTTGA AAGAGTGCCT TTATGTGCTA 
GTGAAAGAAC AGCAAACTCT TGCTACACGG ATGGCTACAA CAACTCTTTC AGCAGTAAGG 
CTAAAGCAGA GGCTGGTTAT ACTGGAACGA TATTTCATTG CACTGAATAG AACTGTTCTT 
CAGGAAAATG TCAAAGTTAA GTGGAAAAGC AACAGTATTC CTTTACCTAC TGTCGACAAA 
AAAAGTTCTA GACCTATAGG AAAAGGTGTA GAAGGACTTG CAAGAGTTGG ATCCAGAGCA 
GCCCTTTCAT TTGCTTTTGC ATTCCTACGC AGAGCTTGGC GGTCAGGGGA AGATGCCGAT 
CTATGCAGTG AATTGTTACA GGAGTCTCTT GATGCCTTGA GAGCCCTACC AGAAGCATCT 
CTTTTTGATG AAAGCACAGT GTCATCTGTT TGGTTGGAAG TTGTGGAGAG AGCAACTAAA 
TTCCTAAGTG ATGTTCATGG AACTGCAAGT ACCAAAGGAC CAGGAAACAT TCCATTACAA 
GACCAGCACT TAGCACTTGC CATATTGTTA GAGTTGGCTG TGCAGAGAGG CACTCTAAGC 
CAAATGTTAT CTGCCATTAT GTTACTACTT CAATTATGGG ACAATGGGAC AAGGGAAACA 
GATAATGAAC GATCTGCCCA AGGAACTAGT GCACCACTTC TCCCTTTATT ACAAAGATTT 
CAGAGTATAA TCTGCAATAA AGACATACTT AATACTGAAG ATGAGATTCA GATGCTGTCT 
GGTCTTCTGA GTCCCAATGA AAGTTTTTTG AGGTACCTTA CTTTACCACA AGACAATGAA 
TTAGCCATTG ATTTGCGTCA AACTGCAGTA GTGATTATGG CGCATTTAGA TCGTCTGGCC 
ACTCCCTGTA TGCCTCCTCA GTGTAGTTCT CCAACATCTC ATAAGGGATC TTTACAAGAA 
GTCATAGGTT GGGGCTTAAT AGGATGGAAA TACTATGCTA ATGTGATTGG CCCAATTCAA 
TGTGAAGGAC TTGCAAATCT TGGGATCACA CAGATTGCCT GTGCAGAAAA GCGTTTCCTG 
ATTTTGTCTC GGAATGGGCG AGTTTACATG CAGGCTTACA ACAGTGACAC ATTGGGACCC 
CAACTAGTTC AAGGTCTTGC CTCTAGAAAT ATTGTGAAAA TTGCAGCACA TTCAGATGGT 
CATCATTATC TGGCTTTGGC AGCCACTGGA GAGGTCTTTT CTTGGGGCTG TGGAGATGGT 
GGACGGTTAG GCCATGGTGA TACAGTGCCT CTAGAAGAAC CTAAGATGAT ATCTGCTCTC 
TCAGGAAAGC AAGCTGGGAA ACATGTAGTT CATATCGCAT GTGGAAGTAC TTATAGTGCA 
GCTATTACAG CTGAAGGAGA GTTATATACT TGGGGTCGAG GAAACTATGG CAGGCTGGGA 
CATGGCTCAA GTGAAGATCA GACAATTCCA ATGCTAGTAA CTGGACTTAA AGGACTGAAG 
GTTATTGATG TCTCTTGTGG GAGTGGGGAT GCTCAGACTC TTGCAGTGAC TGAGAATGGT 
CAAGTCTGGT CTTGGGGCGA TGGTGATTAT GGAAAACTAG GAAGAGGTGG TAGTGATGGT 
TGTAAAACAC CAAAACTGAT TGAAAAACTT CAAGACTTAG ATGTTGTTAA AGTACGTTGT 
GGAAGCCAAT TTTCCGTTGC TTTAACAAAA GATGGTCAAG TTTACTCGTG GGGAAAAGGT 
GACAATCAGA GACTTGGGCA TGGAACAGAG GAGCATGTTC GATATCCGAA ACTGTTAGAA 
GGCTTACAAG GTAAAAAAGT CGTTGATGTG GCTGCTGGTT CTACACACTG CTTAGCACTT 
ACTGAGGATA GCGAAGTTCA TAGCTGGGGG AGTAATGATC AGTGTCAGCA CTTTGACACC 
CTTCGGATTA CCAAACCTGA ACCTACTGCA TTGCCAGGAT TAGATACAAA ACATATAGTT 
GGAATTGCTT GTGGGCCCGC TCAGAGTTTT GCTTGGTCTT CTTGTTCTGA GTGGTCCATT 
GGCCTACGTG TACCTTTTGT GGTGGATGTT TGTCCCATGA CATTTGAACA GTTGGATCTC 
TTACTACGAC AAGTAAGTGA GGGAATGGAT GGCACCTCTG ACTGGCCACC ACCACAGGAG 
AAAGAATGTA TGGCAGTGGC AACATTGAAC CTTCTGAGAC TTCAGTTGCA TGCTGCTATT 
AGTCACCAGG TTGATCCGGA ATGTCTTGGA TTGGGTCTGG GAAGTGTACT CTTGAATAGC 
TTGAAGCAGA CAGTTGTAAC ACTAGCAAGT AATGCTGGAG TTCTTAATAC AGTGCAATCA 
GCTGCTCAAG CAGTACTGCA GAGTGGATGG TCTGTGCTGC TTCCAACTGC AGAGGAACGA 
GCAAGGGCTC TTTCAGCTCT CTTACCCAGT GCAGTTTCAG GAAATGACAT GAATGTAAGT 
CCAGGACGTC GTTTTATGAT TGATCTTCTT GTAGGCAGTT TGATGGCTGA TGGAGGATTA 
GAATCAGCAT TAAATGCTGC CATCACTGCA GAAATCCAGG ATATTGAAGC AAAAAAAGAA 
GCTCAAAAGG AAAAAGAGAT TGATGAGCAG GAAGCAAATG CTTCAACACT TCATCGGAGT 
AGGACACCAT TAGATAAAGA CCTCATTAAT ACTGGAATTT ATGAATCTGC CGGAAAACAG 
AGTTTGCCTC TGGTTCAATT AATACAACAG CTTCTTAGAA ATATTGCTTC CCAGACAATA 
GCCAAATTGA AAGATGTTGC TCGTCGAATT TTGTCCTGTA TAGAACATGA TCAATATAGT 
CAAGACAGAT CTGCCTCCCT GGATTTGTTG CTTCGTTTTC AGCGTCTGCT GGTCAGTAAA 
CTCTATCCAG GAGACAGTGG TGGTCAAGTT TCAAATTCCT TTAGTCCTGA GCTATTAGGA 
GTTGGTTCTT TGCTGAAAAA ATATACAGCT CTTTTGTGCA CACACATTGG AGATATATTG 
CCTGTGGCAA CTAGTATTGC ATCTTCCAGT TATCGGCATT TTGCAGAAGT ATCTCGTGTT 
GTGGATGGTG ATCTAACAGG TGTTCTTCTT CCAGAGTTGA TAGTATCTAT TGTCCTTTTG 
CTCAATAAAA GTCCTGGATT AATGCAGGAA GCTGGGGCAA TTCCTCTCTT AGCTGGTCTA 
CTGGAACATC TAGATAGATT TAACCATTTG GCACCAGGAA AAGAGAGAGA TGATAATGAG 
GATTTAGCCT GGCCTGGAAT AATGGGATCA TTTTTTACAG GTCAGAATAG TAGAAATAAT 
GAGGAAGTGA CGCTCATACG TAAAGCTGAT CTGGAGAATC ACAATAAAGA TGGAGGCTTC 
TGGACTGTGA TTGACGGGAA AGTGTATGAT ATAAAAGACT TTCAGACACA GTCTTTAACA 
GGCAATAGCA TTCTTGCTCA GTTTGCAGGT GATGACCCGG TGGTTGCTTT GGAAGCTGCT 
TTACAGTTTG AGGACACAAG GGATTCAATG CATGCATTTT GTGTTGGCCA GTATATGGAG 
CCTGACCAGG AAGTGATTAC AACGCCAGAC CTTACCAGTC TCTCTTCACC TCTCATAGAT 
CCAGAAAGAA ATCTGGGTCT CCTCCTTGGA CTTCATGCTT CCTATTTAGC AATGAGCACA 
CCACTATCTC CTGTGGAGAT TGAATGTGCC AAATGGCTTA AGTCATCCAT ATTCTCTGGA 
GGATTGCAAA CCAGTCAGAT ACATTATAGT TACAATGAAG AGAAAGATGA AGACCACTGC 
AGTTCTCCTG GAAATACTAC TCCAAACAAA TCCAAACTCT ATTCCCATAG ATTGACTTTG 
AGTGATCATT CCCAACCATT TTTGCAAGCC ATTGCAGATA ATAACATTCA GGATCATACC 
GTGAAGGATT TTTTATGTCA GATAGAAAGG TATTGCAAGC AATGCCATTT GACCACACCG 
ATAACATTTC CTCCTGAACA TCCTGTGGAA GAAGTGGGAC GTTTACTCTT ATGTTGTCTT 
CTAAAGCATG AAGATTTAGG TCATGTGGCC TTATCTTTAG TTCACCCAGT TACACTTGGT 
GTTGACCAAG TCAAACACAG AACGTTGCCT AAGTCTGTAG TGGATGTTTG TAGAGTCGTC 
TACCAGGCAA AATGCTCACT AATAAAGACA CATCAGGAAC AGGGACGTTC TTATAAGGAA 
GTGTGTGCTC CTGTTATTGA ACGTTTGCGA TTTCTTTTTA ATGAATTGCG TCCTGCAGTC 
TGCAATGACC TCTCAATAAT GTCCAAACTT AAACTTTTAA GCTCTTTGCC TCGTTGGAGA 
AGAATAGCTC AGAAGATGAT ACGAGAGAGA AGGAAATTAA AAAGAGTACC CAAAAAGTCA 
GAACCTACTG ATGTGGAAGA ATCTAAGATT GGCAATGAAG AGGGTGACTT AGAAGAAGCC 
TGTATTCTTC CTCATAGTCC TGTATCTGTA GATAAAAGAC CTGTTCCAGT TAAATCACCT 
AAGGATAAAT GGCAGCCACT TCTGAGTACA GTTACAGGTG TTCACAAATA CAAATGGCTT 
AAACAGAATG TTCAAGGCCT TTATCCTCAG TCTGCACTGC TTAGCACAAT TGTTGAATTT 
GCGCTTAAAG AAGAGCCAGT TGATGTGGAA AAGATGAGAA AGTGCTTACT CAAACAGTTG 
GAGAGAGCAG AAGTTCGTTT GGAAGGAATA GACACGATTT TAAAATTGGC AAATAAAAAT 
TTCTTGCTTT CGTCTGTGCA ATATGCTATG TTCTGTGGAT GGCAAAGACT TATCCCAGAA 
GGAACCAATA TAGGGGAACC ACTTACAGAT TGTTTAAAGG ATGTTGATCT GATTCCACCA 
TTTAATCGAA TGCTACTAGA AGTAACTTTT GGAAAGTTGT ATGGTTGGGC CATTCAGAAT 
ATTCGAAATG TTTTGTTGGA TGCAAATGCC AGATTTAAAG AGCTAGGTAT CCAGCCTGTT 
CCTCTGCAAA CTATTACTAA TGAGAATCCA GCAGGGCCAA GCCTTGGAAC CATCCCACAA 
GCACGTTTTC TCCTGGTTAT GCTCAACATG CTTACCCTTC AATATGGTGC AAACAACTTG 
AATCTTCTTT TGAACTCTGG CATGCTAGCA TTGACTCAGA CAACATTGCG ATTAATAGGT 
CCTAGTTCTG ATAATATCGA GGAAGAAATG AATGCCTCTG CACGAGGAGC TTCAGCTACT 
GTTTTGGAAG AATCCAGAAA AGAAACCACT CCAGTACAAC TTCCTGTTTC AGGGCCAGAA 
CTGGCAGCAA TGATGAAAAT TGGTACCAGG GTCATGAGGG GAGTGGACTG GAAATGGGGA 
GATCAGGATG GTCCACCTCC AGGTTTAGGT CGTGTGATTG GTGAATTGGG AGAGGATGGA 
TGGATAAGGG TTCAGTGGGA TACAGGCAGT ACTAATTCAT ACCGAATGGG AAAGGAAGGA 
AAGTATGATC TTAAATTGGC TGAGCCTCCA CCTGCAGCTC AGCCCTCAAC AGAGGATTCG 
GACACTGAGG ATGATTCTGA AAGTGAACAA ATGGAAAGAA ACTTCCACCC TACTTCTATT 
ATGCTTACAA GTACTGTTAA TTTACTCCAA ACTCTCTGTC TCTGTGCTGG AATTCATGCA 
GAAATCATGC AAAATGAAGC TACAAGAACT CTGTGTGGTC TACTTAGAAT GTTAGTGGAA 
AGTGGAACAG TAGATAAAAC ATCTTCTTTA CCAAATAAGA TGGTTTATAA AGAGCAGCAC 
AGAAGTTGGT GTACACTTGG ATTTATTCGA AGTATAGCAC TTATGCCTCA AATGTGTAGC 
ACCCTTAGCA CATCTCAGTG GATCACATTA CTAATGAAAA TTGTGGAAGG ACACGAGTCA 
TTTACAGCAG CTTCGCTTCA GAGACAGATC TTAGCAGTAC ATTTACTACA AGCTGTACTT 
CCATCTTGGG ACAAAACAGA AAGATCAAGA GATATGAAGT TTCTTGTGGA AAAACTTTTT 
GGCTTCTTGG GCAGTCTACT TAGTACATGC TCTTCAGACA TACCTTTGCT CAGAGAATCA 
ACTCTGAGAA GGAGGAAGTC CAGACCCCAG GCATCTTTGA CTGCAACCCA TAGTAGTACT 
TTGGCAGAGG AGATAGTGGC CCTGCTAAGG ACTCTACATT CACTGAGCCA GTGGAATGGA 
CTCATAAACA AGTACATCAA CTCTCAGCTA AACTCCATCA CCCACATCTT TGCAGGAAAA 
CAGTCTGAAG GGACTGTGTT AGAAGATTAC TTTCCTGATT CTGAGAACCC TGAAGTTGAT 
GGCCTAATGG CAGTGCTAGC TGTAATTGGT GGAATAGATA GTCGATTGAG GCTGGGTGGC 
CAAGTGGTAC ATGATGAATT TGGAGAAGGC ACAGTGACAC GCATCACTCC TAAAGGCAAA 
ATCACAGTCC AGTTCTGTGA TATGCGAATA TGTAGAATTT GTCCATTGAG TCAGCTAAAA 
CCGCTTCCAG TTATGGCATT TAATGTGAAC AACTTACCAT TCACGGAATC TATGCTCTCT 
GTCTGGGCTC AATTGGTCAA CTTGGCAGGG AGCAAATTAG AAAAACACAG AATTAAGAAA 
TCTACTAATC AAGCCCTCAC AGGACAAGTA GACTTGGATT TGCTAAGATG CCAACAGTTG 
AAATTATACA TATTGAAAGC AGGTCGAGCA TTGCTTTCCC ATCAGGATAA ACTGAGGCAG 
ATCTTATCTC AACCAGCTTT ACAAGAGAAT GGAGTTCTCT CTACAGATGA TGGTACAGCA 
TCCCCTGATA TTGGAGATAT GTCTCCTGAA GGACCTCAAC CTCCAATGAT CCTTTTACAG 
CAATTGTTGA CATCTGCCAC ACAGCCATCA CCTGTAAAAG CAATATTTGA CAAACAGGAA 
CTTGAGGCTG CTGCTTTGGC TGTATGCCAG TGTCTAGCTG TGGAGTCAAC TCATCCATCA 
ACTCCAGTGT TTGAAGAATG TAGCTCTAGT GAGGCCACAA CACCTGTCAC AGTCCAGCAT 
ATTCGATCTG TAAAAGTGAA AAAGCGAAAG CAGTCTCCTC TCCCTGCTTT GCCTATTGTA 
GTACAGCTCA TGGAAATGGG ATTTCCGAGG AAAAACATTG AATTTGCATT GAAGTCTCTT 
ACTGGGAGTG CTTCAGGGTT ACCAGGTGTT GAAGCCTTGG TTGGTTGGTT GCTGGATCAT 
CCTGATGTCC AAATTACTGA TCTCTCAGAT GCTGATACAG TCTCTGATGA ATACTCTGAT 
GATGAAATAG TGGAAGATGT GGAAGAGACT GAAGCATCTT ACCCTGTGTC TAGTTGTGCT 
GTTGTAACGG AGAGTCACAC TTATAAAAAA CGAGCTGATT TCTTAAGTAA TGATGACTAT 
GCAGTATATG TTAGAGAAAA CATTCAGGTA GGAATGATGG TTAGGTGCTG CAGAACGTAT 
GAAGAAGTTT GTGAAGGAGA TGTAGGTAAA GTGATTAAAT TGGACAGAGA TGGATTACAC 
GACCTCAATG TGCAGTGTGA TTGGCAACAA AAGGGGAGTA CCTATTGGGT TAGGTATATC 
CATGTTGAAC TTATAGGTTT CCCACCACAG AGTTCTCCTT CCCATATCAA GATTGGTGAT 
AAAGTTAGGG TGAAAACATC AGTAACCACA CCAAAATATA AATGGGGATC AGTTACTCAT 
AGAAGTGTGG GAGTTGTGAA AGCTTTTAGT GCCAATGGAA AAGATGTTAT TGTGGATTTT 
CCTCAGCAGT CTCATTGGAC AGGATTATTG TCAGAAATGG AATTGGTGCC TAGTATTCAT 
CCTGGAGTTA CGTGTGATGG CTGCCAAATG TTTCCTATCA ATGGTCCTCG TTTCAAATGC 
AGGAGTTGTG ATGATTTTGA CTTTTGTGAG ACCTGTTTTA AAACCAAGAA GCATAATACC 
AGACATAGTT TTGGCAGAAT AAATGAACCA GGTCAGTCTC TTGTATTTTG TGGGCGTTCT 
GGGAAACAGC TAAAACGTTG CCACAGTAGT CAGCCTGGAA TGCTCTTGGA TAATTGGTCA 
AGAATTGTTA AGAATTTAAA TGTGTCCTCA TCAGTAAATC AGGCTTCTCG CTTGATTGAT 
GGCAGTGAAC CATGTTGGCA GTCATCTGGA TCACAAGGAA AGCATTGGAT TCGACTGGAG 
ATTTTCCCCG ATGTTCTTGT TCATAGACTG AAAATGATAG TAGATCCTGC TGACAGTAGC 
TACATGCCTT CACTAGTTGT AGTTTCAGGT GGGAATTCCC TAAATAATTT AATTGAACTG 
AAGACAATCA ATATTAACCC AGCTGATACC ACAGTGCCTC TTCTAAATGA CTGCACAGAG 
TATCACAGGT ATATTGAGGT TGCCATAAAG CAATGCAGAA GCTCAGGAAT AGATTGTAAA 
ATTCATGGTC TTATTATACT GGGACGAATT CGGGCAGAAG ATGAAGATTT GGCTGCTGTT 
CCTTTCTTAG CGTCAGATAA TGAAGAAGAA GATGATGAAA AAGCTAACAC TGGGAGTCTT 
ATTAGGAAGA AGGCTTCTGG ATTGGAATCA GCAGCCACAA TAAGGACCAA AGTTTTTGTC 
TGGGGGCTGA ATGACAAGGA TCAATTAGGA GGATTAAAGG GTTCTAAGAT AAAAGTTCCT 
TCATTCTCTG AAACCCTTTC TGCTTTGAAT GTAGTTCAAG TGGCAGGTGG ATCTAAAAGT 
TTGTTTGCAG TGACTGTGGA GGGTAAAGTG TATGCTTGTG GAGAAGCTAC TAATGGACGA 
CTGGGTTTAG GTATTTCTAG TGGAACTGTA CCCATTCCGC GGCAGATTAC AGCTCTCAGC 
AATTATGTGG TAAAGAAAGT TGCTGTTCAC TCAGGTGGCC GGCATGCTAT GGCTTTAACT 
GTTGATGGAA AGATATTTTC ATGGGGTGAA GGTGATGATG GAAAACTAGG ACACTTCAGC 
AGAATGAACT GTGATAAACC AAGACTGATA GAGGCATTGA AAACCAAACG TATTCGTGAT 
ATAGCTTGTG GTAGTTCTCA CAGTGCTGCC ATTACATCAA GTGGTGAATT GTATACATGG 
GGCCTTGGAG AATATGGCCG GTTGGGACAT GGGGATAATA CTACACAGCT GAAACCCAAA 
ATGGTGAAAG TTCTTCTTGG TCATCGAGTT ATTCAAGTTG CTTGTGGAAG TAGAGATGCC 
CAGACTCTGG CTTTGACAGA TGAAGGTTTG GTCTTTTCCT GGGGAGATGG TGACTTTGGG 
AAACTGGGCC GGGGAGGAAG TGAAGGATGT AACATTCCTC AGAACATTGA GAGACTTAAT 
GGACAAGGAG TTTGCCAGAT TGAGTGTGGG GCTCAATTTT CCTTGGCACT TACAAAGTCA 
GGAGTAGTAT GGACTTGGGG TAAAGGAGAT TACTTTAGGC TTGGTCATGG CACTGATGTT 
CATGTGAGAA AACCACAAGT AGTAGAAGGA CTACGAGGAA AGAAGATAGT TCATGTGGCA 
GTAGGCGCTC TTCACTGCTT AGCTGTCACT GATACAGGTC AAGTGTATGC TTGGGGTGAC 
AATGATCATG GACAACAAGG CAATGGGACT ACTACTGTCA ACAGAAAGCC TACCCTTGTA 
CAAGGCTTAG AAGGACAAAA AATCACTCGG GTAGCTTGTG GCTCATCTCA TAGTGTGGCG 
TGGACAACAG TGGATGTAGC AACCCCATCT GTTCATGAAC CTGTCCTCTT TCAAACTGCT 
AGGGACCCTT TAGGCGCTTC TTACTTAGGT GTTCCTTCCG ATGCTGATTC TTCTGCTGCC 
AGTAACAAAA TCAGTGGTGT AAATAGTTCC AAGCCTAACC GCCCTTCCCT TGCTAAGATT 
CTCTTGTCTT TGGATGGAAA CCTTGCCAAA CAGCAAGCAT TATCTCATAT ACTTACAGCA 
TTGCAAATTA TGTATGCGAG AGATGCAGTA GTTGGAGCAT TAATGCCTGC TAGTATGATC 
GCACCAGTGG AATGCTCATC TTCTTCATCA CCTGCACCCC CCTCAGATGT GTCTTCAGCA 
GGAAGTCCCA TGAATGGTGA AGAGTCTGTG TTAGCTGTGG ATGTAGAAGA TAGACTAAGC 
CCAAATCCAT GGCAAGACAA GAGAGGGGAG GTAGTTTCTT CAGAAGATGC TGTGACTCCA 
TCAGCAGTGA CTCCCTCAGC TTCCTCTGCC TCTTCTCGAC CATTTATTCC AGTAACTGAT 
GATCCTGGAG CTGCCAGTAT CATTGCAGAA ACCATGACTA AAACCAAAGA AGATGTTGAA 
AGCCAAAATA AAGTATCAGG TCCGGAACCT CAATATTTGG ATGAATTTAC TAGTCTCTTA 
GTGCCAGATG ATACCCGTGT CATGGTTGAC TTGCTCAAGC TGTCTGTTTC TAATCGAGCT 
GGTGAAAAGG GAAAAGATGT TCTTTCAGCT GTGCTTTCTG GTATGGGCAC TGCTTATCCA 
CAGGTAGCTG ATATGTTGTT GGAGCTCTGT GTTACAGAGT TAGAAGATGT TGCCACAGAC 
TCTCAGAGTG GTCGACTCTC TTCCCAACCT GTTGTAGTAG AAAGTAGTCA TCCGTATACT 
GATGATACCT CTACCAGTGG TACTGTCAAA ATACCAGGTG CTGAAGGACT CAGAGTAGAG 
TTTGATCGAC AATGTTCTAC AGAACGGCGT CATGACCCTC TCACAATCAT GGATGGTGTC 
AATAGGATAG TTTCAGTTCG ATCAGGTCGA GAATGGTCTG ATTGGTCCAG TGAACTTCGA 
ATTCCTGGAG ATGAATTAAA GTGGAAGTTC ATCAGTGATG GATCAGTGAA TGGATGGGGT 
TGGCGATTCA CTGTCTATCC TATTATGCCA GCTGCAGGCC CTAAAGATCT TCTTTCAGAT 
CGGTGTATTC TTTCCTGTCC CTCAATGGAC TTGGTTACAT GCTTGTTGGA CTTTAGATTA 
AATTTAGCTT CCAATAGAAG TATTGTTCCT CGTTTGGCAG CATCACTTGC AGCATGTGCT 
CAATTGAGTG CTCTAGCTGC TGGTCACAGA ATGTGGGCCC TTCAAAGACT GAGGAAACTG 
CTTACAACTG AATTTGGACA ATCAATCAAT ATAAATAGGA TACTTGGAGA TAATGATGGA 
GAAACAAGAA CCATGAGTTT TACCGGTAGT GCTTTAGCAG CTTTGGTAAA AGGTCTTCCA 
GAAGCTTTGC AAAGACAGTT TGAATATGAA GATCCGATTG TTAGAGGAGG CAAACAACTG 
CTCCATAGCC CTTTCTTTAA GGTGCTGGTA GCTCTTGCTT GTGACCTAGA ACTGGATACT 
CTTCCTTGCT GTGCTGAGAC ACACAAATGG GCTTGGTTTA GAAGATACTG TATGGCCTCT 
AGAGTTTCAG TAGCCTTGGA CAAACGAACA CCATTACCTC GCCTTTTCCT TGATGAGGTG 
GCAAAGAAAA TCAGGGAGTT AATGGCCGAC AGTGAAAATC TGGATGTTCT GCATGAGAGC 
CATGACATTT TTAAGAGAGA ACAAGATGAA CAACTCGTCC AGTGGATGAA CAGGAGACCT 
GATGACTGGA CTCTTTCTGC TGGAGGCAGT GGAACAATTT ATGGTTGGGG TCACAATCAT 
CGAGGACAGC TTGGCGGTAT TGAGGGAGCA AAAGTCAAAG TTCCAACACC TTGTGAAGCT 
CTTGCAGCTC TGCGGCCCAT ACAGTTAATT GGAGGGGAAC AGACTCTCTT TGCGGTAACT 
GCTGATGGGA AGCTTTATGC TACAGGGTAT GGTGCAGGTG GCAGATTGGG AATTGGAGGA 
ACTGAATCAG TTTCTACTCC AACATTGCTT GAATCTATTC AGCATGTATT TATAAAAAAA 
GTTGCTGTGA ACTCTGGAGG AAAACACTGC TTAGCACTCT CATCAGAAGG AGAAGTTTAT 
TCTTGGGGTG AGGCAGAAGA TGGAAAATTG GGGCATGGAA ACAGAAGCCC CTGTGATCGC 
CCACGTGTAA TTGAATCTTT GCGAGGAATA GAAGTTGTTG ATATTGCTGC AGGTGGAGCC 
CACAGTGCAT GTATTACAGC AGCTGGTGAC CTCTATACGT GGGGTAAAGG AAGATATGGT 
CGCCTAGGTC ATGGGGATAG TGAAGATCAG TTAAAACCAA AACTGGTGGA AGCTCTCCAA 
GGCTATCGAG TTATTGATAT TGCCTGTGGC AGTGGAGATG CACAGACACT TTGCCTTACT 
GATGATGATA CTGTATGGTC CTGGGGTGAT GGGGACTATG GAAAACTTGG CAGAGGAGGC 
AGTGATGGCT GTAAAGTGCC AATGAAGATT GATTCTCTCA CAGGTCTTGG AGTGGTTAAA 
GTAGAGTGTG GATCACAATT TTCTGTTGCA CTTACCAAGT CTGGAGCAGT TTATACTTGG 
GGTAAAGGCG ATTATCACAG GTTGGGTCAT GGATCAGACG ACCATGTTAG ACGGCCTCGG 
CAAGTGCAAG GCCTACAAGG AAAGAAAGTA ATAGCAATTG CTACTGGTTC TTTACACTGT 
GTGTGCTGCA CTGAAGACGG TGAAGTTTAT ACTTGGGGTG ACAATGATGA AGGGCAACTT 
GGGGATGGAA CAACTAATGC AATCCAGAGA CCTCGGTTGG TGGCAGCACT TCAGGGTAAA 
AAGATAAATA GGGTGGCTTG TGGTTCAGCA CACACCTTAG CGTGGTCCAC GAGTAAACCT 
GCCAATGCTG GGAAGCTTCC AACACAGGTT CCTATGGAAT ACAACCATTT ACAGGAATTT 
CTCATAATTC CTTTACGAAA CAGACTTTTG TTATTACATC ATATCTCAGA ACTCTTTTGT 
CCATGCATTC CCATGTTTGA CCTTGAGGGG CGTCTTGATG AAACTGGCCA AGGACCTTCA 
GTGGGATTTG ACACATTACG AGGAATTCTG ATATCTCAGG GAAAGGAAGC AGCTTTCCGA 
AAAGTTGTAC AAGCAACTAT GGTGAGAGAT CGTCAACATG GTCCTGTAGT TGAATTAAAC 
AGAATACAGG TAAAACGATC AAGAAGTAAG GGTGGTTTGG CTGGTCCTGA TGGTACTAAA 
TCTGTTTTTG GCCAAATGTG TGCTAAAATG ATTTCATTTA GTCCAGACAG TCTTCTTCTA 
CCTCATCGAG TATGGAAAGT CAAGTTTGTT GGCGAGTCTG TGGATGATTG TGGAGGTGGT 
TATAGTGAAT CAATAGCAGA AATGTGTGAA GAACTTCAGA ATGGACTTAC TCCTCTCCTA 
ATTGTAACTC CAAATGGCAG GGATGAATCT GGAGCCAATA GAGATTGCTT CTTATTGAAC 
CCAGCTGCCA GATCTTCTTT GCATACCAAT ATGTTTCGTT TCTTGGGTGT ATTGCTAGGC 
ATTGCCATTC GAACTGGGAG TCCATTAAGC CTCAACCTTG CAGAACCGGT TTGGAAGCAA 
CTGGCAGGAA TGAACCTTAC AATTGCTGAT CTCAGTGAGG TTGATAAGGA TTTTATTCCT 
GGCCTGATGT ACATTCGGGA CAATGAAGCT ACTTCTGAGG AATTTGAAGC AATGAGCCTT 
CCCTTCACAG TGCCAAGTGC AAGTGGGCAG GATATTCAAT TGAGCTCTAA ATATACCCAC 
ATCACATTGG ATAACCGTGC TGAGTATGTT AGATTGGCAA TAAATTACAG ACTCCATGAA 
TTTGATGAAC AAGTTGCTGC AGTTCGGGAA GGAATGGCTC GAGTTGTCCC TGTTCCTCTT 
CTTTCACTCT TTACTGGATA TGAACTGGAA ACTATGGTGT GTGGAAGCCC CGATATTCCA 
CTTCATCTTC TAAAATCTGT AGCCACATAT AAAGGAATTG AACCTACTGC TTCTTTGATC 
CAGTGGTTCT GGGAAGTGAT GGAATCTTTC TCTAATACAG AGCGCTCTCT CTTTCTTCGT 
TTTGTGTGGG GCAGAACACG ACTTCCTAGA ACAATTGCTG ATTTCAGAGG CAGAGATTTT 
GTTATTCAGG TTTTGGATAA GTACAATCCA CCTGACCACT TCCTTCCAGA GTCATATACA 
TGCTTCTTTT TGCTGAAGTT ACCCAGGTAT TCCTGTAAAC AAGTACTAGA GGAAAAACTG 
AAATATGCCA TTCATTTTTG CAAGTCTATA GACACTGATG ATTATGCCCG GATAGCACTC 
ACAGGAGAAC CAGCTGCTGA TGACAGCAGT GATGATTCAG ATAATGAAGA TGCAGATTCA 
TTTGCATCGG ACTCTACTCA AGATTATTTA ACAGGACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC2 ( XM_031959090.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031814950.1 Sarcophilus harrisii HECT and RLD domain containing E3 ubiquitin protein ligase 2 (HERC2), transcript variant X3, mRNA.

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CloneID	OSa229207
Clone ID Related Accession (Same CDS sequence)	XM_031959090.1
Accession Version	XM_031959090.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	E3 ubiquitin-protein ligase HERC2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045428.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAAGG TAGTGTACTT AGATCGTCTG GCCACTCCCT GCATGCCTCC TCAGTGTAGT 
TCTCCTACAT CTCATAAGGG ATCTTTACAA GAAGTCATAG GTTGGGGCTT AATAGGATGG 
AAATACTATG CTAATGTGAT TGGCCCAATT CAATGTGAAG GACTTGCAAA TCTTGGGATC 
ACACAGATTG CCTGTGCAGA AAAGCGTTTC CTGATTTTGT CTCGGAATGG GCGAGTTTAC 
ATGCAGGCTT ACAACAGTGA CACATTGGGA CCCCAACTAG TTCAAGGTCT TGCCTCTAGA 
AATATTGTGA AAATTGCAGC ACATTCAGAT GGTCATCATT ATCTGGCTTT GGCAGCCACT 
GGAGAGGTCT TTTCTTGGGG CTGTGGAGAT GGTGGACGGT TAGGCCATGG TGATACAGTG 
CCTCTAGAAG AACCTAAGAT GATATCTGCT CTCTCAGGAA AGCAAGCTGG GAAACATGTA 
GTTCATATCG CATGTGGAAG TACTTATAGT GCAGCTATTA CAGCTGAAGG AGAGTTATAT 
ACTTGGGGTC GAGGAAACTA TGGCAGGCTG GGACATGGCT CAAGTGAAGA TCAGACAATT 
CCAATGCTAG TAACTGGACT TAAAGGACTG AAGGTTATTG ATGTCTCTTG TGGGAGTGGG 
GATGCTCAGA CTCTTGCAGT GACTGAGAAT GGTCAAGTCT GGTCTTGGGG CGATGGTGAT 
TATGGAAAAC TAGGAAGAGG TGGTAGTGAT GGTTGTAAAA CACCAAAACT GATTGAAAAA 
CTTCAAGACT TAGATGTTGT TAAAGTACGT TGTGGAAGCC AATTTTCCGT TGCTTTAACA 
AAAGATGGTC AAGTTTACTC GTGGGGAAAA GGTGACAATC AGAGACTTGG GCATGGAACA 
GAGGAGCATG TTCGATATCC GAAACTGTTA GAAGGCTTAC AAGGTAAAAA AGTCGTTGAT 
GTGGCTGCTG GTTCTACACA CTGCTTAGCA CTTACTGAGG ATAGCGAAGT TCATAGCTGG 
GGGAGTAATG ATCAGTGTCA GCACTTTGAC ACCCTTCGGA TTACCAAACC TGAACCTACT 
GCATTGCCAG GATTAGATAC AAAACATATA GTTGGAATTG CTTGTGGGCC CGCTCAGAGT 
TTTGCTTGGT CTTCTTGTTC TGAGTGGTCC ATTGGCCTAC GTGTACCTTT TGTGGTGGAT 
GTTTGTCCCA TGACATTTGA ACAGTTGGAT CTCTTACTAC GACAAGTAAG TGAGGGAATG 
GATGGCACCT CTGACTGGCC ACCACCACAG GAGAAAGAAT GTATGGCAGT GGCAACATTG 
AACCTTCTGA GACTTCAGTT GCATGCTGCT ATTAGTCACC AGGTTGATCC GGAATGTCTT 
GGATTGGGTC TGGGAAGTGT ACTCTTGAAT AGCTTGAAGC AGACAGTTGT AACACTAGCA 
AGTAATGCTG GAGTTCTTAA TACAGTGCAA TCAGCTGCTC AAGCAGTACT GCAGAGTGGA 
TGGTCTGTGC TGCTTCCAAC TGCAGAGGAA CGAGCAAGGG CTCTTTCAGC TCTCTTACCC 
AGTGCAGTTT CAGGAAATGA CATGAATGTA AGTCCAGGAC GTCGTTTTAT GATTGATCTT 
CTTGTAGGCA GTTTGATGGC TGATGGAGGA TTAGAATCAG CATTAAATGC TGCCATCACT 
GCAGAAATCC AGGATATTGA AGCAAAAAAA GAAGCTCAAA AGGAAAAAGA GATTGATGAG 
CAGGAAGCAA ATGCTTCAAC ACTTCATCGG AGTAGGACAC CATTAGATAA AGACCTCATT 
AATACTGGAA TTTATGAATC TGCCGGAAAA CAGAGTTTGC CTCTGGTTCA ATTAATACAA 
CAGCTTCTTA GAAATATTGC TTCCCAGACA ATAGCCAAAT TGAAAGATGT TGCTCGTCGA 
ATTTTGTCCT GTATAGAACA TGATCAATAT AGTCAAGACA GATCTGCCTC CCTGGATTTG 
TTGCTTCGTT TTCAGCGTCT GCTGGTCAGT AAACTCTATC CAGGAGACAG TGGTGGTCAA 
GTTTCAAATT CCTTTAGTCC TGAGCTATTA GGAGTTGGTT CTTTGCTGAA AAAATATACA 
GCTCTTTTGT GCACACACAT TGGAGATATA TTGCCTGTGG CAACTAGTAT TGCATCTTCC 
AGTTATCGGC ATTTTGCAGA AGTATCTCGT GTTGTGGATG GTGATCTAAC AGGTGTTCTT 
CTTCCAGAGT TGATAGTATC TATTGTCCTT TTGCTCAATA AAAGTCCTGG ATTAATGCAG 
GAAGCTGGGG CAATTCCTCT CTTAGCTGGT CTACTGGAAC ATCTAGATAG ATTTAACCAT 
TTGGCACCAG GAAAAGAGAG AGATGATAAT GAGGATTTAG CCTGGCCTGG AATAATGGGA 
TCATTTTTTA CAGGTCAGAA TAGTAGAAAT AATGAGGAAG TGACGCTCAT ACGTAAAGCT 
GATCTGGAGA ATCACAATAA AGATGGAGGC TTCTGGACTG TGATTGACGG GAAAGTGTAT 
GATATAAAAG ACTTTCAGAC ACAGTCTTTA ACAGGCAATA GCATTCTTGC TCAGTTTGCA 
GGTGATGACC CGGTGGTTGC TTTGGAAGCT GCTTTACAGT TTGAGGACAC AAGGGATTCA 
ATGCATGCAT TTTGTGTTGG CCAGTATATG GAGCCTGACC AGGAAGTGAT TACAACGCCA 
GACCTTACCA GTCTCTCTTC ACCTCTCATA GATCCAGAAA GAAATCTGGG TCTCCTCCTT 
GGACTTCATG CTTCCTATTT AGCAATGAGC ACACCACTAT CTCCTGTGGA GATTGAATGT 
GCCAAATGGC TTAAGTCATC CATATTCTCT GGAGGATTGC AAACCAGTCA GATACATTAT 
AGTTACAATG AAGAGAAAGA TGAAGACCAC TGCAGTTCTC CTGGAAATAC TACTCCAAAC 
AAATCCAAAC TCTATTCCCA TAGATTGACT TTGAGTGATC ATTCCCAACC ATTTTTGCAA 
GCCATTGCAG ATAATAACAT TCAGGATCAT ACCGTGAAGG ATTTTTTATG TCAGATAGAA 
AGGTATTGCA AGCAATGCCA TTTGACCACA CCGATAACAT TTCCTCCTGA ACATCCTGTG 
GAAGAAGTGG GACGTTTACT CTTATGTTGT CTTCTAAAGC ATGAAGATTT AGGTCATGTG 
GCCTTATCTT TAGTTCACCC AGTTACACTT GGTGTTGACC AAGTCAAACA CAGAACGTTG 
CCTAAGTCTG TAGTGGATGT TTGTAGAGTC GTCTACCAGG CAAAATGCTC ACTAATAAAG 
ACACATCAGG AACAGGGACG TTCTTATAAG GAAGTGTGTG CTCCTGTTAT TGAACGTTTG 
CGATTTCTTT TTAATGAATT GCGTCCTGCA GTCTGCAATG ACCTCTCAAT AATGTCCAAA 
CTTAAACTTT TAAGCTCTTT GCCTCGTTGG AGAAGAATAG CTCAGAAGAT GATACGAGAG 
AGAAGGAAAT TAAAAAGAGT ACCCAAAAAG TCAGAACCTA CTGATGTGGA AGAATCTAAG 
ATTGGCAATG AAGAGGGTGA CTTAGAAGAA GCCTGTATTC TTCCTCATAG TCCTGTATCT 
GTAGATAAAA GACCTGTTCC AGTTAAATCA CCTAAGGATA AATGGCAGCC ACTTCTGAGT 
ACAGTTACAG GTGTTCACAA ATACAAATGG CTTAAACAGA ATGTTCAAGG CCTTTATCCT 
CAGTCTGCAC TGCTTAGCAC AATTGTTGAA TTTGCGCTTA AAGAAGAGCC AGTTGATGTG 
GAAAAGATGA GAAAGTGCTT ACTCAAACAG TTGGAGAGAG CAGAAGTTCG TTTGGAAGGA 
ATAGACACGA TTTTAAAATT GGCAAATAAA AATTTCTTGC TTTCGTCTGT GCAATATGCT 
ATGTTCTGTG GATGGCAAAG ACTTATCCCA GAAGGAACCA ATATAGGGGA ACCACTTACA 
GATTGTTTAA AGGATGTTGA TCTGATTCCA CCATTTAATC GAATGCTACT AGAAGTAACT 
TTTGGAAAGT TGTATGGTTG GGCCATTCAG AATATTCGAA ATGTTTTGTT GGATGCAAAT 
GCCAGATTTA AAGAGCTAGG TATCCAGCCT GTTCCTCTGC AAACTATTAC TAATGAGAAT 
CCAGCAGGGC CAAGCCTTGG AACCATCCCA CAAGCACGTT TTCTCCTGGT TATGCTCAAC 
ATGCTTACCC TTCAATATGG TGCAAACAAC TTGAATCTTC TTTTGAACTC TGGCATGCTA 
GCATTGACTC AGACAACATT GCGATTAATA GGTCCTAGTT CTGATAATAT CGAGGAAGAA 
ATGAATGCCT CTGCACGAGG AGCTTCAGCT ACTGTTTTGG AAGAATCCAG AAAAGAAACC 
ACTCCAGTAC AACTTCCTGT TTCAGGGCCA GAACTGGCAG CAATGATGAA AATTGGTACC 
AGGGTCATGA GGGGAGTGGA CTGGAAATGG GGAGATCAGG ATGGTCCACC TCCAGGTTTA 
GGTCGTGTGA TTGGTGAATT GGGAGAGGAT GGATGGATAA GGGTTCAGTG GGATACAGGC 
AGTACTAATT CATACCGAAT GGGAAAGGAA GGAAAGTATG ATCTTAAATT GGCTGAGCCT 
CCACCTGCAG CTCAGCCCTC AACAGAGGAT TCGGACACTG AGGATGATTC TGAAAGTGAA 
CAAATGGAAA GAAACTTCCA CCCTACTTCT ATTATGCTTA CAAGTACTGT TAATTTACTC 
CAAACTCTCT GTCTCTGTGC TGGAATTCAT GCAGAAATCA TGCAAAATGA AGCTACAAGA 
ACTCTGTGTG GTCTACTTAG AATGTTAGTG GAAAGTGGAA CAGTAGATAA AACATCTTCT 
TTACCAAATA AGATGGTTTA TAAAGAGCAG CACAGAAGTT GGTGTACACT TGGATTTATT 
CGAAGTATAG CACTTATGCC TCAAATGTGT AGCACCCTTA GCACATCTCA GTGGATCACA 
TTACTAATGA AAATTGTGGA AGGACACGAG TCATTTACAG CAGCTTCGCT TCAGAGACAG 
ATCTTAGCAG TACATTTACT ACAAGCTGTA CTTCCATCTT GGGACAAAAC AGAAAGATCA 
AGAGATATGA AGTTTCTTGT GGAAAAACTT TTTGGCTTCT TGGGCAGTCT ACTTAGTACA 
TGCTCTTCAG ACATACCTTT GCTCAGAGAA TCAACTCTGA GAAGGAGGAA GTCCAGACCC 
CAGGCATCTT TGACTGCAAC CCATAGTAGT ACTTTGGCAG AGGAGATAGT GGCCCTGCTA 
AGGACTCTAC ATTCACTGAG CCAGTGGAAT GGACTCATAA ACAAGTACAT CAACTCTCAG 
CTAAACTCCA TCACCCACAT CTTTGCAGGA AAACAGTCTG AAGGGACTGT GTTAGAAGAT 
TACTTTCCTG ATTCTGAGAA CCCTGAAGTT GATGGCCTAA TGGCAGTGCT AGCTGTAATT 
GGTGGAATAG ATAGTCGATT GAGGCTGGGT GGCCAAGTGG TACATGATGA ATTTGGAGAA 
GGCACAGTGA CACGCATCAC TCCTAAAGGC AAAATCACAG TCCAGTTCTG TGATATGCGA 
ATATGTAGAA TTTGTCCATT GAGTCAGCTA AAACCGCTTC CAGTTATGGC ATTTAATGTG 
AACAACTTAC CATTCACGGA ATCTATGCTC TCTGTCTGGG CTCAATTGGT CAACTTGGCA 
GGGAGCAAAT TAGAAAAACA CAGAATTAAG AAATCTACTA ATCAAGCCCT CACAGGACAA 
GTAGACTTGG ATTTGCTAAG ATGCCAACAG TTGAAATTAT ACATATTGAA AGCAGGTCGA 
GCATTGCTTT CCCATCAGGA TAAACTGAGG CAGATCTTAT CTCAACCAGC TTTACAAGAG 
AATGGAGTTC TCTCTACAGA TGATGGTACA GCATCCCCTG ATATTGGAGA TATGTCTCCT 
GAAGGACCTC AACCTCCAAT GATCCTTTTA CAGCAATTGT TGACATCTGC CACACAGCCA 
TCACCTGTAA AAGCAATATT TGACAAACAG GAACTTGAGG CTGCTGCTTT GGCTGTATGC 
CAGTGTCTAG CTGTGGAGTC AACTCATCCA TCAACTCCAG TGTTTGAAGA ATGTAGCTCT 
AGTGAGGCCA CAACACCTGT CACAGTCCAG CATATTCGAT CTGTAAAAGT GAAAAAGCGA 
AAGCAGTCTC CTCTCCCTGC TTTGCCTATT GTAGTACAGC TCATGGAAAT GGGATTTCCG 
AGGAAAAACA TTGAATTTGC ATTGAAGTCT CTTACTGGGA GTGCTTCAGG GTTACCAGGT 
GTTGAAGCCT TGGTTGGTTG GTTGCTGGAT CATCCTGATG TCCAAATTAC TGATCTCTCA 
GATGCTGATA CAGTCTCTGA TGAATACTCT GATGATGAAA TAGTGGAAGA TGTGGAAGAG 
ACTGAAGCAT CTTACCCTGT GTCTAGTTGT GCTGTTGTAA CGGAGAGTCA CACTTATAAA 
AAACGAGCTG ATTTCTTAAG TAATGATGAC TATGCAGTAT ATGTTAGAGA AAACATTCAG 
GTAGGAATGA TGGTTAGGTG CTGCAGAACG TATGAAGAAG TTTGTGAAGG AGATGTAGGT 
AAAGTGATTA AATTGGACAG AGATGGATTA CACGACCTCA ATGTGCAGTG TGATTGGCAA 
CAAAAGGGGA GTACCTATTG GGTTAGGTAT ATCCATGTTG AACTTATAGG TTTCCCACCA 
CAGAGTTCTC CTTCCCATAT CAAGATTGGT GATAAAGTTA GGGTGAAAAC ATCAGTAACC 
ACACCAAAAT ATAAATGGGG ATCAGTTACT CATAGAAGTG TGGGAGTTGT GAAAGCTTTT 
AGTGCCAATG GAAAAGATGT TATTGTGGAT TTTCCTCAGC AGTCTCATTG GACAGGATTA 
TTGTCAGAAA TGGAATTGGT GCCTAGTATT CATCCTGGAG TTACGTGTGA TGGCTGCCAA 
ATGTTTCCTA TCAATGGTCC TCGTTTCAAA TGCAGGAGTT GTGATGATTT TGACTTTTGT 
GAGACCTGTT TTAAAACCAA GAAGCATAAT ACCAGACATA GTTTTGGCAG AATAAATGAA 
CCAGGTCAGT CTCTTGTATT TTGTGGGCGT TCTGGGAAAC AGCTAAAACG TTGCCACAGT 
AGTCAGCCTG GAATGCTCTT GGATAATTGG TCAAGAATTG TTAAGAATTT AAATGTGTCC 
TCATCAGTAA ATCAGGCTTC TCGCTTGATT GATGGCAGTG AACCATGTTG GCAGTCATCT 
GGATCACAAG GAAAGCATTG GATTCGACTG GAGATTTTCC CCGATGTTCT TGTTCATAGA 
CTGAAAATGA TAGTAGATCC TGCTGACAGT AGCTACATGC CTTCACTAGT TGTAGTTTCA 
GGTGGGAATT CCCTAAATAA TTTAATTGAA CTGAAGACAA TCAATATTAA CCCAGCTGAT 
ACCACAGTGC CTCTTCTAAA TGACTGCACA GAGTATCACA GGTATATTGA GGTTGCCATA 
AAGCAATGCA GAAGCTCAGG AATAGATTGT AAAATTCATG GTCTTATTAT ACTGGGACGA 
ATTCGGGCAG AAGATGAAGA TTTGGCTGCT GTTCCTTTCT TAGCGTCAGA TAATGAAGAA 
GAAGATGATG AAAAAGCTAA CACTGGGAGT CTTATTAGGA AGAAGGCTTC TGGATTGGAA 
TCAGCAGCCA CAATAAGGAC CAAAGTTTTT GTCTGGGGGC TGAATGACAA GGATCAATTA 
GGAGGATTAA AGGGTTCTAA GATAAAAGTT CCTTCATTCT CTGAAACCCT TTCTGCTTTG 
AATGTAGTTC AAGTGGCAGG TGGATCTAAA AGTTTGTTTG CAGTGACTGT GGAGGGTAAA 
GTGTATGCTT GTGGAGAAGC TACTAATGGA CGACTGGGTT TAGGTATTTC TAGTGGAACT 
GTACCCATTC CGCGGCAGAT TACAGCTCTC AGCAATTATG TGGTAAAGAA AGTTGCTGTT 
CACTCAGGTG GCCGGCATGC TATGGCTTTA ACTGTTGATG GAAAGATATT TTCATGGGGT 
GAAGGTGATG ATGGAAAACT AGGACACTTC AGCAGAATGA ACTGTGATAA ACCAAGACTG 
ATAGAGGCAT TGAAAACCAA ACGTATTCGT GATATAGCTT GTGGTAGTTC TCACAGTGCT 
GCCATTACAT CAAGTGGTGA ATTGTATACA TGGGGCCTTG GAGAATATGG CCGGTTGGGA 
CATGGGGATA ATACTACACA GCTGAAACCC AAAATGGTGA AAGTTCTTCT TGGTCATCGA 
GTTATTCAAG TTGCTTGTGG AAGTAGAGAT GCCCAGACTC TGGCTTTGAC AGATGAAGGT 
TTGGTCTTTT CCTGGGGAGA TGGTGACTTT GGGAAACTGG GCCGGGGAGG AAGTGAAGGA 
TGTAACATTC CTCAGAACAT TGAGAGACTT AATGGACAAG GAGTTTGCCA GATTGAGTGT 
GGGGCTCAAT TTTCCTTGGC ACTTACAAAG TCAGGAGTAG TATGGACTTG GGGTAAAGGA 
GATTACTTTA GGCTTGGTCA TGGCACTGAT GTTCATGTGA GAAAACCACA AGTAGTAGAA 
GGACTACGAG GAAAGAAGAT AGTTCATGTG GCAGTAGGCG CTCTTCACTG CTTAGCTGTC 
ACTGATACAG GTCAAGTGTA TGCTTGGGGT GACAATGATC ATGGACAACA AGGCAATGGG 
ACTACTACTG TCAACAGAAA GCCTACCCTT GTACAAGGCT TAGAAGGACA AAAAATCACT 
CGGGTAGCTT GTGGCTCATC TCATAGTGTG GCGTGGACAA CAGTGGATGT AGCAACCCCA 
TCTGTTCATG AACCTGTCCT CTTTCAAACT GCTAGGGACC CTTTAGGCGC TTCTTACTTA 
GGTGTTCCTT CCGATGCTGA TTCTTCTGCT GCCAGTAACA AAATCAGTGG TGTAAATAGT 
TCCAAGCCTA ACCGCCCTTC CCTTGCTAAG ATTCTCTTGT CTTTGGATGG AAACCTTGCC 
AAACAGCAAG CATTATCTCA TATACTTACA GCATTGCAAA TTATGTATGC GAGAGATGCA 
GTAGTTGGAG CATTAATGCC TGCTAGTATG ATCGCACCAG TGGAATGCTC ATCTTCTTCA 
TCACCTGCAC CCCCCTCAGA TGTGTCTTCA GCAGGAAGTC CCATGAATGG TGAAGAGTCT 
GTGTTAGCTG TGGATGTAGA AGATAGACTA AGCCCAAATC CATGGCAAGA CAAGAGAGGG 
GAGGTAGTTT CTTCAGAAGA TGCTGTGACT CCATCAGCAG TGACTCCCTC AGCTTCCTCT 
GCCTCTTCTC GACCATTTAT TCCAGTAACT GATGATCCTG GAGCTGCCAG TATCATTGCA 
GAAACCATGA CTAAAACCAA AGAAGATGTT GAAAGCCAAA ATAAAGTATC AGGTCCGGAA 
CCTCAATATT TGGATGAATT TACTAGTCTC TTAGTGCCAG ATGATACCCG TGTCATGGTT 
GACTTGCTCA AGCTGTCTGT TTCTAATCGA GCTGGTGAAA AGGGAAAAGA TGTTCTTTCA 
GCTGTGCTTT CTGGTATGGG CACTGCTTAT CCACAGGTAG CTGATATGTT GTTGGAGCTC 
TGTGTTACAG AGTTAGAAGA TGTTGCCACA GACTCTCAGA GTGGTCGACT CTCTTCCCAA 
CCTGTTGTAG TAGAAAGTAG TCATCCGTAT ACTGATGATA CCTCTACCAG TGGTACTGTC 
AAAATACCAG GTGCTGAAGG ACTCAGAGTA GAGTTTGATC GACAATGTTC TACAGAACGG 
CGTCATGACC CTCTCACAAT CATGGATGGT GTCAATAGGA TAGTTTCAGT TCGATCAGGT 
CGAGAATGGT CTGATTGGTC CAGTGAACTT CGAATTCCTG GAGATGAATT AAAGTGGAAG 
TTCATCAGTG ATGGATCAGT GAATGGATGG GGTTGGCGAT TCACTGTCTA TCCTATTATG 
CCAGCTGCAG GCCCTAAAGA TCTTCTTTCA GATCGGTGTA TTCTTTCCTG TCCCTCAATG 
GACTTGGTTA CATGCTTGTT GGACTTTAGA TTAAATTTAG CTTCCAATAG AAGTATTGTT 
CCTCGTTTGG CAGCATCACT TGCAGCATGT GCTCAATTGA GTGCTCTAGC TGCTGGTCAC 
AGAATGTGGG CCCTTCAAAG ACTGAGGAAA CTGCTTACAA CTGAATTTGG ACAATCAATC 
AATATAAATA GGATACTTGG AGATAATGAT GGAGAAACAA GAACCATGAG TTTTACCGGT 
AGTGCTTTAG CAGCTTTGGT AAAAGGTCTT CCAGAAGCTT TGCAAAGACA GTTTGAATAT 
GAAGATCCGA TTGTTAGAGG AGGCAAACAA CTGCTCCATA GCCCTTTCTT TAAGGTGCTG 
GTAGCTCTTG CTTGTGACCT AGAACTGGAT ACTCTTCCTT GCTGTGCTGA GACACACAAA 
TGGGCTTGGT TTAGAAGATA CTGTATGGCC TCTAGAGTTT CAGTAGCCTT GGACAAACGA 
ACACCATTAC CTCGCCTTTT CCTTGATGAG GTGGCAAAGA AAATCAGGGA GTTAATGGCC 
GACAGTGAAA ATCTGGATGT TCTGCATGAG AGCCATGACA TTTTTAAGAG AGAACAAGAT 
GAACAACTCG TCCAGTGGAT GAACAGGAGA CCTGATGACT GGACTCTTTC TGCTGGAGGC 
AGTGGAACAA TTTATGGTTG GGGTCACAAT CATCGAGGAC AGCTTGGCGG TATTGAGGGA 
GCAAAAGTCA AAGTTCCAAC ACCTTGTGAA GCTCTTGCAG CTCTGCGGCC CATACAGTTA 
ATTGGAGGGG AACAGACTCT CTTTGCGGTA ACTGCTGATG GGAAGCTTTA TGCTACAGGG 
TATGGTGCAG GTGGCAGATT GGGAATTGGA GGAACTGAAT CAGTTTCTAC TCCAACATTG 
CTTGAATCTA TTCAGCATGT ATTTATAAAA AAAGTTGCTG TGAACTCTGG AGGAAAACAC 
TGCTTAGCAC TCTCATCAGA AGGAGAAGTT TATTCTTGGG GTGAGGCAGA AGATGGAAAA 
TTGGGGCATG GAAACAGAAG CCCCTGTGAT CGCCCACGTG TAATTGAATC TTTGCGAGGA 
ATAGAAGTTG TTGATATTGC TGCAGGTGGA GCCCACAGTG CATGTATTAC AGCAGCTGGT 
GACCTCTATA CGTGGGGTAA AGGAAGATAT GGTCGCCTAG GTCATGGGGA TAGTGAAGAT 
CAGTTAAAAC CAAAACTGGT GGAAGCTCTC CAAGGCTATC GAGTTATTGA TATTGCCTGT 
GGCAGTGGAG ATGCACAGAC ACTTTGCCTT ACTGATGATG ATACTGTATG GTCCTGGGGT 
GATGGGGACT ATGGAAAACT TGGCAGAGGA GGCAGTGATG GCTGTAAAGT GCCAATGAAG 
ATTGATTCTC TCACAGGTCT TGGAGTGGTT AAAGTAGAGT GTGGATCACA ATTTTCTGTT 
GCACTTACCA AGTCTGGAGC AGTTTATACT TGGGGTAAAG GCGATTATCA CAGGTTGGGT 
CATGGATCAG ACGACCATGT TAGACGGCCT CGGCAAGTGC AAGGCCTACA AGGAAAGAAA 
GTAATAGCAA TTGCTACTGG TTCTTTACAC TGTGTGTGCT GCACTGAAGA CGGTGAAGTT 
TATACTTGGG GTGACAATGA TGAAGGGCAA CTTGGGGATG GAACAACTAA TGCAATCCAG 
AGACCTCGGT TGGTGGCAGC ACTTCAGGGT AAAAAGATAA ATAGGGTGGC TTGTGGTTCA 
GCACACACCT TAGCGTGGTC CACGAGTAAA CCTGCCAATG CTGGGAAGCT TCCAACACAG 
GTTCCTATGG AATACAACCA TTTACAGGAA TTTCTCATAA TTCCTTTACG AAACAGACTT 
TTGTTATTAC ATCATATCTC AGAACTCTTT TGTCCATGCA TTCCCATGTT TGACCTTGAG 
GGGCGTCTTG ATGAAACTGG CCAAGGACCT TCAGTGGGAT TTGACACATT ACGAGGAATT 
CTGATATCTC AGGGAAAGGA AGCAGCTTTC CGAAAAGTTG TACAAGCAAC TATGGTGAGA 
GATCGTCAAC ATGGTCCTGT AGTTGAATTA AACAGAATAC AGGTAAAACG ATCAAGAAGT 
AAGGGTGGTT TGGCTGGTCC TGATGGTACT AAATCTGTTT TTGGCCAAAT GTGTGCTAAA 
ATGATTTCAT TTAGTCCAGA CAGTCTTCTT CTACCTCATC GAGTATGGAA AGTCAAGTTT 
GTTGGCGAGT CTGTGGATGA TTGTGGAGGT GGTTATAGTG AATCAATAGC AGAAATGTGT 
GAAGAACTTC AGAATGGACT TACTCCTCTC CTAATTGTAA CTCCAAATGG CAGGGATGAA 
TCTGGAGCCA ATAGAGATTG CTTCTTATTG AACCCAGCTG CCAGATCTTC TTTGCATACC 
AATATGTTTC GTTTCTTGGG TGTATTGCTA GGCATTGCCA TTCGAACTGG GAGTCCATTA 
AGCCTCAACC TTGCAGAACC GGTTTGGAAG CAACTGGCAG GAATGAACCT TACAATTGCT 
GATCTCAGTG AGGTTGATAA GGATTTTATT CCTGGCCTGA TGTACATTCG GGACAATGAA 
GCTACTTCTG AGGAATTTGA AGCAATGAGC CTTCCCTTCA CAGTGCCAAG TGCAAGTGGG 
CAGGATATTC AATTGAGCTC TAAATATACC CACATCACAT TGGATAACCG TGCTGAGTAT 
GTTAGATTGG CAATAAATTA CAGACTCCAT GAATTTGATG AACAAGTTGC TGCAGTTCGG 
GAAGGAATGG CTCGAGTTGT CCCTGTTCCT CTTCTTTCAC TCTTTACTGG ATATGAACTG 
GAAACTATGG TGTGTGGAAG CCCCGATATT CCACTTCATC TTCTAAAATC TGTAGCCACA 
TATAAAGGAA TTGAACCTAC TGCTTCTTTG ATCCAGTGGT TCTGGGAAGT GATGGAATCT 
TTCTCTAATA CAGAGCGCTC TCTCTTTCTT CGTTTTGTGT GGGGCAGAAC ACGACTTCCT 
AGAACAATTG CTGATTTCAG AGGCAGAGAT TTTGTTATTC AGGTTTTGGA TAAGTACAAT 
CCACCTGACC ACTTCCTTCC AGAGTCATAT ACATGCTTCT TTTTGCTGAA GTTACCCAGG 
TATTCCTGTA AACAAGTACT AGAGGAAAAA CTGAAATATG CCATTCATTT TTGCAAGTCT 
ATAGACACTG ATGATTATGC CCGGATAGCA CTCACAGGAG AACCAGCTGC TGATGACAGC 
AGTGATGATT CAGATAATGA AGATGCAGAT TCATTTGCAT CGGACTCTAC TCAAGATTAT 
TTAACAGGAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031814950.1
CDS	912..14246
Translation	MWKVVYLDRLATPCMPPQCSSPTSHKGSLQEVIGWGLIGWKYYANVIGPIQCEGLANLGITQIACAEKRFLILSRNGRVYMQAYNSDTLGPQLVQGLASRNIVKIAAHSDGHHYLALAATGEVFSWGCGDGGRLGHGDTVPLEEPKMISALSGKQAGKHVVHIACGSTYSAAITAEGELYTWGRGNYGRLGHGSSEDQTIPMLVTGLKGLKVIDVSCGSGDAQTLAVTENGQVWSWGDGDYGKLGRGGSDGCKTPKLIEKLQDLDVVKVRCGSQFSVALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVVDVAAGSTHCLALTEDSEVHSWGSNDQCQHFDTLRITKPEPTALPGLDTKHIVGIACGPAQSFAWSSCSEWSIGLRVPFVVDVCPMTFEQLDLLLRQVSEGMDGTSDWPPPQEKECMAVATLNLLRLQLHAAISHQVDPECLGLGLGSVLLNSLKQTVVTLASNAGVLNTVQSAAQAVLQSGWSVLLPTAEERARALSALLPSAVSGNDMNVSPGRRFMIDLLVGSLMADGGLESALNAAITAEIQDIEAKKEAQKEKEIDEQEANASTLHRSRTPLDKDLINTGIYESAGKQSLPLVQLIQQLLRNIASQTIAKLKDVARRILSCIEHDQYSQDRSASLDLLLRFQRLLVSKLYPGDSGGQVSNSFSPELLGVGSLLKKYTALLCTHIGDILPVATSIASSSYRHFAEVSRVVDGDLTGVLLPELIVSIVLLLNKSPGLMQEAGAIPLLAGLLEHLDRFNHLAPGKERDDNEDLAWPGIMGSFFTGQNSRNNEEVTLIRKADLENHNKDGGFWTVIDGKVYDIKDFQTQSLTGNSILAQFAGDDPVVALEAALQFEDTRDSMHAFCVGQYMEPDQEVITTPDLTSLSSPLIDPERNLGLLLGLHASYLAMSTPLSPVEIECAKWLKSSIFSGGLQTSQIHYSYNEEKDEDHCSSPGNTTPNKSKLYSHRLTLSDHSQPFLQAIADNNIQDHTVKDFLCQIERYCKQCHLTTPITFPPEHPVEEVGRLLLCCLLKHEDLGHVALSLVHPVTLGVDQVKHRTLPKSVVDVCRVVYQAKCSLIKTHQEQGRSYKEVCAPVIERLRFLFNELRPAVCNDLSIMSKLKLLSSLPRWRRIAQKMIRERRKLKRVPKKSEPTDVEESKIGNEEGDLEEACILPHSPVSVDKRPVPVKSPKDKWQPLLSTVTGVHKYKWLKQNVQGLYPQSALLSTIVEFALKEEPVDVEKMRKCLLKQLERAEVRLEGIDTILKLANKNFLLSSVQYAMFCGWQRLIPEGTNIGEPLTDCLKDVDLIPPFNRMLLEVTFGKLYGWAIQNIRNVLLDANARFKELGIQPVPLQTITNENPAGPSLGTIPQARFLLVMLNMLTLQYGANNLNLLLNSGMLALTQTTLRLIGPSSDNIEEEMNASARGASATVLEESRKETTPVQLPVSGPELAAMMKIGTRVMRGVDWKWGDQDGPPPGLGRVIGELGEDGWIRVQWDTGSTNSYRMGKEGKYDLKLAEPPPAAQPSTEDSDTEDDSESEQMERNFHPTSIMLTSTVNLLQTLCLCAGIHAEIMQNEATRTLCGLLRMLVESGTVDKTSSLPNKMVYKEQHRSWCTLGFIRSIALMPQMCSTLSTSQWITLLMKIVEGHESFTAASLQRQILAVHLLQAVLPSWDKTERSRDMKFLVEKLFGFLGSLLSTCSSDIPLLRESTLRRRKSRPQASLTATHSSTLAEEIVALLRTLHSLSQWNGLINKYINSQLNSITHIFAGKQSEGTVLEDYFPDSENPEVDGLMAVLAVIGGIDSRLRLGGQVVHDEFGEGTVTRITPKGKITVQFCDMRICRICPLSQLKPLPVMAFNVNNLPFTESMLSVWAQLVNLAGSKLEKHRIKKSTNQALTGQVDLDLLRCQQLKLYILKAGRALLSHQDKLRQILSQPALQENGVLSTDDGTASPDIGDMSPEGPQPPMILLQQLLTSATQPSPVKAIFDKQELEAAALAVCQCLAVESTHPSTPVFEECSSSEATTPVTVQHIRSVKVKKRKQSPLPALPIVVQLMEMGFPRKNIEFALKSLTGSASGLPGVEALVGWLLDHPDVQITDLSDADTVSDEYSDDEIVEDVEETEASYPVSSCAVVTESHTYKKRADFLSNDDYAVYVRENIQVGMMVRCCRTYEEVCEGDVGKVIKLDRDGLHDLNVQCDWQQKGSTYWVRYIHVELIGFPPQSSPSHIKIGDKVRVKTSVTTPKYKWGSVTHRSVGVVKAFSANGKDVIVDFPQQSHWTGLLSEMELVPSIHPGVTCDGCQMFPINGPRFKCRSCDDFDFCETCFKTKKHNTRHSFGRINEPGQSLVFCGRSGKQLKRCHSSQPGMLLDNWSRIVKNLNVSSSVNQASRLIDGSEPCWQSSGSQGKHWIRLEIFPDVLVHRLKMIVDPADSSYMPSLVVVSGGNSLNNLIELKTININPADTTVPLLNDCTEYHRYIEVAIKQCRSSGIDCKIHGLIILGRIRAEDEDLAAVPFLASDNEEEDDEKANTGSLIRKKASGLESAATIRTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALNVVQVAGGSKSLFAVTVEGKVYACGEATNGRLGLGISSGTVPIPRQITALSNYVVKKVAVHSGGRHAMALTVDGKIFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAAITSSGELYTWGLGEYGRLGHGDNTTQLKPKMVKVLLGHRVIQVACGSRDAQTLALTDEGLVFSWGDGDFGKLGRGGSEGCNIPQNIERLNGQGVCQIECGAQFSLALTKSGVVWTWGKGDYFRLGHGTDVHVRKPQVVEGLRGKKIVHVAVGALHCLAVTDTGQVYAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSVAWTTVDVATPSVHEPVLFQTARDPLGASYLGVPSDADSSAASNKISGVNSSKPNRPSLAKILLSLDGNLAKQQALSHILTALQIMYARDAVVGALMPASMIAPVECSSSSSPAPPSDVSSAGSPMNGEESVLAVDVEDRLSPNPWQDKRGEVVSSEDAVTPSAVTPSASSASSRPFIPVTDDPGAASIIAETMTKTKEDVESQNKVSGPEPQYLDEFTSLLVPDDTRVMVDLLKLSVSNRAGEKGKDVLSAVLSGMGTAYPQVADMLLELCVTELEDVATDSQSGRLSSQPVVVESSHPYTDDTSTSGTVKIPGAEGLRVEFDRQCSTERRHDPLTIMDGVNRIVSVRSGREWSDWSSELRIPGDELKWKFISDGSVNGWGWRFTVYPIMPAAGPKDLLSDRCILSCPSMDLVTCLLDFRLNLASNRSIVPRLAASLAACAQLSALAAGHRMWALQRLRKLLTTEFGQSININRILGDNDGETRTMSFTGSALAALVKGLPEALQRQFEYEDPIVRGGKQLLHSPFFKVLVALACDLELDTLPCCAETHKWAWFRRYCMASRVSVALDKRTPLPRLFLDEVAKKIRELMADSENLDVLHESHDIFKREQDEQLVQWMNRRPDDWTLSAGGSGTIYGWGHNHRGQLGGIEGAKVKVPTPCEALAALRPIQLIGGEQTLFAVTADGKLYATGYGAGGRLGIGGTESVSTPTLLESIQHVFIKKVAVNSGGKHCLALSSEGEVYSWGEAEDGKLGHGNRSPCDRPRVIESLRGIEVVDIAAGGAHSACITAAGDLYTWGKGRYGRLGHGDSEDQLKPKLVEALQGYRVIDIACGSGDAQTLCLTDDDTVWSWGDGDYGKLGRGGSDGCKVPMKIDSLTGLGVVKVECGSQFSVALTKSGAVYTWGKGDYHRLGHGSDDHVRRPRQVQGLQGKKVIAIATGSLHCVCCTEDGEVYTWGDNDEGQLGDGTTNAIQRPRLVAALQGKKINRVACGSAHTLAWSTSKPANAGKLPTQVPMEYNHLQEFLIIPLRNRLLLLHHISELFCPCIPMFDLEGRLDETGQGPSVGFDTLRGILISQGKEAAFRKVVQATMVRDRQHGPVVELNRIQVKRSRSKGGLAGPDGTKSVFGQMCAKMISFSPDSLLLPHRVWKVKFVGESVDDCGGGYSESIAEMCEELQNGLTPLLIVTPNGRDESGANRDCFLLNPAARSSLHTNMFRFLGVLLGIAIRTGSPLSLNLAEPVWKQLAGMNLTIADLSEVDKDFIPGLMYIRDNEATSEEFEAMSLPFTVPSASGQDIQLSSKYTHITLDNRAEYVRLAINYRLHEFDEQVAAVREGMARVVPVPLLSLFTGYELETMVCGSPDIPLHLLKSVATYKGIEPTASLIQWFWEVMESFSNTERSLFLRFVWGRTRLPRTIADFRGRDFVIQVLDKYNPPDHFLPESYTCFFLLKLPRYSCKQVLEEKLKYAIHFCKSIDTDDYARIALTGEPAADDSSDDSDNEDADSFASDSTQDYLTGH

Target ORF information:

RefSeq Version	XM_031959090.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HECT and RLD domain containing E3 ubiquitin protein ligase 2 (HERC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031959090.1

ORF Insert Sequence:

ATGTGGAAGG TAGTGTACTT AGATCGTCTG GCCACTCCCT GCATGCCTCC TCAGTGTAGT 
TCTCCTACAT CTCATAAGGG ATCTTTACAA GAAGTCATAG GTTGGGGCTT AATAGGATGG 
AAATACTATG CTAATGTGAT TGGCCCAATT CAATGTGAAG GACTTGCAAA TCTTGGGATC 
ACACAGATTG CCTGTGCAGA AAAGCGTTTC CTGATTTTGT CTCGGAATGG GCGAGTTTAC 
ATGCAGGCTT ACAACAGTGA CACATTGGGA CCCCAACTAG TTCAAGGTCT TGCCTCTAGA 
AATATTGTGA AAATTGCAGC ACATTCAGAT GGTCATCATT ATCTGGCTTT GGCAGCCACT 
GGAGAGGTCT TTTCTTGGGG CTGTGGAGAT GGTGGACGGT TAGGCCATGG TGATACAGTG 
CCTCTAGAAG AACCTAAGAT GATATCTGCT CTCTCAGGAA AGCAAGCTGG GAAACATGTA 
GTTCATATCG CATGTGGAAG TACTTATAGT GCAGCTATTA CAGCTGAAGG AGAGTTATAT 
ACTTGGGGTC GAGGAAACTA TGGCAGGCTG GGACATGGCT CAAGTGAAGA TCAGACAATT 
CCAATGCTAG TAACTGGACT TAAAGGACTG AAGGTTATTG ATGTCTCTTG TGGGAGTGGG 
GATGCTCAGA CTCTTGCAGT GACTGAGAAT GGTCAAGTCT GGTCTTGGGG CGATGGTGAT 
TATGGAAAAC TAGGAAGAGG TGGTAGTGAT GGTTGTAAAA CACCAAAACT GATTGAAAAA 
CTTCAAGACT TAGATGTTGT TAAAGTACGT TGTGGAAGCC AATTTTCCGT TGCTTTAACA 
AAAGATGGTC AAGTTTACTC GTGGGGAAAA GGTGACAATC AGAGACTTGG GCATGGAACA 
GAGGAGCATG TTCGATATCC GAAACTGTTA GAAGGCTTAC AAGGTAAAAA AGTCGTTGAT 
GTGGCTGCTG GTTCTACACA CTGCTTAGCA CTTACTGAGG ATAGCGAAGT TCATAGCTGG 
GGGAGTAATG ATCAGTGTCA GCACTTTGAC ACCCTTCGGA TTACCAAACC TGAACCTACT 
GCATTGCCAG GATTAGATAC AAAACATATA GTTGGAATTG CTTGTGGGCC CGCTCAGAGT 
TTTGCTTGGT CTTCTTGTTC TGAGTGGTCC ATTGGCCTAC GTGTACCTTT TGTGGTGGAT 
GTTTGTCCCA TGACATTTGA ACAGTTGGAT CTCTTACTAC GACAAGTAAG TGAGGGAATG 
GATGGCACCT CTGACTGGCC ACCACCACAG GAGAAAGAAT GTATGGCAGT GGCAACATTG 
AACCTTCTGA GACTTCAGTT GCATGCTGCT ATTAGTCACC AGGTTGATCC GGAATGTCTT 
GGATTGGGTC TGGGAAGTGT ACTCTTGAAT AGCTTGAAGC AGACAGTTGT AACACTAGCA 
AGTAATGCTG GAGTTCTTAA TACAGTGCAA TCAGCTGCTC AAGCAGTACT GCAGAGTGGA 
TGGTCTGTGC TGCTTCCAAC TGCAGAGGAA CGAGCAAGGG CTCTTTCAGC TCTCTTACCC 
AGTGCAGTTT CAGGAAATGA CATGAATGTA AGTCCAGGAC GTCGTTTTAT GATTGATCTT 
CTTGTAGGCA GTTTGATGGC TGATGGAGGA TTAGAATCAG CATTAAATGC TGCCATCACT 
GCAGAAATCC AGGATATTGA AGCAAAAAAA GAAGCTCAAA AGGAAAAAGA GATTGATGAG 
CAGGAAGCAA ATGCTTCAAC ACTTCATCGG AGTAGGACAC CATTAGATAA AGACCTCATT 
AATACTGGAA TTTATGAATC TGCCGGAAAA CAGAGTTTGC CTCTGGTTCA ATTAATACAA 
CAGCTTCTTA GAAATATTGC TTCCCAGACA ATAGCCAAAT TGAAAGATGT TGCTCGTCGA 
ATTTTGTCCT GTATAGAACA TGATCAATAT AGTCAAGACA GATCTGCCTC CCTGGATTTG 
TTGCTTCGTT TTCAGCGTCT GCTGGTCAGT AAACTCTATC CAGGAGACAG TGGTGGTCAA 
GTTTCAAATT CCTTTAGTCC TGAGCTATTA GGAGTTGGTT CTTTGCTGAA AAAATATACA 
GCTCTTTTGT GCACACACAT TGGAGATATA TTGCCTGTGG CAACTAGTAT TGCATCTTCC 
AGTTATCGGC ATTTTGCAGA AGTATCTCGT GTTGTGGATG GTGATCTAAC AGGTGTTCTT 
CTTCCAGAGT TGATAGTATC TATTGTCCTT TTGCTCAATA AAAGTCCTGG ATTAATGCAG 
GAAGCTGGGG CAATTCCTCT CTTAGCTGGT CTACTGGAAC ATCTAGATAG ATTTAACCAT 
TTGGCACCAG GAAAAGAGAG AGATGATAAT GAGGATTTAG CCTGGCCTGG AATAATGGGA 
TCATTTTTTA CAGGTCAGAA TAGTAGAAAT AATGAGGAAG TGACGCTCAT ACGTAAAGCT 
GATCTGGAGA ATCACAATAA AGATGGAGGC TTCTGGACTG TGATTGACGG GAAAGTGTAT 
GATATAAAAG ACTTTCAGAC ACAGTCTTTA ACAGGCAATA GCATTCTTGC TCAGTTTGCA 
GGTGATGACC CGGTGGTTGC TTTGGAAGCT GCTTTACAGT TTGAGGACAC AAGGGATTCA 
ATGCATGCAT TTTGTGTTGG CCAGTATATG GAGCCTGACC AGGAAGTGAT TACAACGCCA 
GACCTTACCA GTCTCTCTTC ACCTCTCATA GATCCAGAAA GAAATCTGGG TCTCCTCCTT 
GGACTTCATG CTTCCTATTT AGCAATGAGC ACACCACTAT CTCCTGTGGA GATTGAATGT 
GCCAAATGGC TTAAGTCATC CATATTCTCT GGAGGATTGC AAACCAGTCA GATACATTAT 
AGTTACAATG AAGAGAAAGA TGAAGACCAC TGCAGTTCTC CTGGAAATAC TACTCCAAAC 
AAATCCAAAC TCTATTCCCA TAGATTGACT TTGAGTGATC ATTCCCAACC ATTTTTGCAA 
GCCATTGCAG ATAATAACAT TCAGGATCAT ACCGTGAAGG ATTTTTTATG TCAGATAGAA 
AGGTATTGCA AGCAATGCCA TTTGACCACA CCGATAACAT TTCCTCCTGA ACATCCTGTG 
GAAGAAGTGG GACGTTTACT CTTATGTTGT CTTCTAAAGC ATGAAGATTT AGGTCATGTG 
GCCTTATCTT TAGTTCACCC AGTTACACTT GGTGTTGACC AAGTCAAACA CAGAACGTTG 
CCTAAGTCTG TAGTGGATGT TTGTAGAGTC GTCTACCAGG CAAAATGCTC ACTAATAAAG 
ACACATCAGG AACAGGGACG TTCTTATAAG GAAGTGTGTG CTCCTGTTAT TGAACGTTTG 
CGATTTCTTT TTAATGAATT GCGTCCTGCA GTCTGCAATG ACCTCTCAAT AATGTCCAAA 
CTTAAACTTT TAAGCTCTTT GCCTCGTTGG AGAAGAATAG CTCAGAAGAT GATACGAGAG 
AGAAGGAAAT TAAAAAGAGT ACCCAAAAAG TCAGAACCTA CTGATGTGGA AGAATCTAAG 
ATTGGCAATG AAGAGGGTGA CTTAGAAGAA GCCTGTATTC TTCCTCATAG TCCTGTATCT 
GTAGATAAAA GACCTGTTCC AGTTAAATCA CCTAAGGATA AATGGCAGCC ACTTCTGAGT 
ACAGTTACAG GTGTTCACAA ATACAAATGG CTTAAACAGA ATGTTCAAGG CCTTTATCCT 
CAGTCTGCAC TGCTTAGCAC AATTGTTGAA TTTGCGCTTA AAGAAGAGCC AGTTGATGTG 
GAAAAGATGA GAAAGTGCTT ACTCAAACAG TTGGAGAGAG CAGAAGTTCG TTTGGAAGGA 
ATAGACACGA TTTTAAAATT GGCAAATAAA AATTTCTTGC TTTCGTCTGT GCAATATGCT 
ATGTTCTGTG GATGGCAAAG ACTTATCCCA GAAGGAACCA ATATAGGGGA ACCACTTACA 
GATTGTTTAA AGGATGTTGA TCTGATTCCA CCATTTAATC GAATGCTACT AGAAGTAACT 
TTTGGAAAGT TGTATGGTTG GGCCATTCAG AATATTCGAA ATGTTTTGTT GGATGCAAAT 
GCCAGATTTA AAGAGCTAGG TATCCAGCCT GTTCCTCTGC AAACTATTAC TAATGAGAAT 
CCAGCAGGGC CAAGCCTTGG AACCATCCCA CAAGCACGTT TTCTCCTGGT TATGCTCAAC 
ATGCTTACCC TTCAATATGG TGCAAACAAC TTGAATCTTC TTTTGAACTC TGGCATGCTA 
GCATTGACTC AGACAACATT GCGATTAATA GGTCCTAGTT CTGATAATAT CGAGGAAGAA 
ATGAATGCCT CTGCACGAGG AGCTTCAGCT ACTGTTTTGG AAGAATCCAG AAAAGAAACC 
ACTCCAGTAC AACTTCCTGT TTCAGGGCCA GAACTGGCAG CAATGATGAA AATTGGTACC 
AGGGTCATGA GGGGAGTGGA CTGGAAATGG GGAGATCAGG ATGGTCCACC TCCAGGTTTA 
GGTCGTGTGA TTGGTGAATT GGGAGAGGAT GGATGGATAA GGGTTCAGTG GGATACAGGC 
AGTACTAATT CATACCGAAT GGGAAAGGAA GGAAAGTATG ATCTTAAATT GGCTGAGCCT 
CCACCTGCAG CTCAGCCCTC AACAGAGGAT TCGGACACTG AGGATGATTC TGAAAGTGAA 
CAAATGGAAA GAAACTTCCA CCCTACTTCT ATTATGCTTA CAAGTACTGT TAATTTACTC 
CAAACTCTCT GTCTCTGTGC TGGAATTCAT GCAGAAATCA TGCAAAATGA AGCTACAAGA 
ACTCTGTGTG GTCTACTTAG AATGTTAGTG GAAAGTGGAA CAGTAGATAA AACATCTTCT 
TTACCAAATA AGATGGTTTA TAAAGAGCAG CACAGAAGTT GGTGTACACT TGGATTTATT 
CGAAGTATAG CACTTATGCC TCAAATGTGT AGCACCCTTA GCACATCTCA GTGGATCACA 
TTACTAATGA AAATTGTGGA AGGACACGAG TCATTTACAG CAGCTTCGCT TCAGAGACAG 
ATCTTAGCAG TACATTTACT ACAAGCTGTA CTTCCATCTT GGGACAAAAC AGAAAGATCA 
AGAGATATGA AGTTTCTTGT GGAAAAACTT TTTGGCTTCT TGGGCAGTCT ACTTAGTACA 
TGCTCTTCAG ACATACCTTT GCTCAGAGAA TCAACTCTGA GAAGGAGGAA GTCCAGACCC 
CAGGCATCTT TGACTGCAAC CCATAGTAGT ACTTTGGCAG AGGAGATAGT GGCCCTGCTA 
AGGACTCTAC ATTCACTGAG CCAGTGGAAT GGACTCATAA ACAAGTACAT CAACTCTCAG 
CTAAACTCCA TCACCCACAT CTTTGCAGGA AAACAGTCTG AAGGGACTGT GTTAGAAGAT 
TACTTTCCTG ATTCTGAGAA CCCTGAAGTT GATGGCCTAA TGGCAGTGCT AGCTGTAATT 
GGTGGAATAG ATAGTCGATT GAGGCTGGGT GGCCAAGTGG TACATGATGA ATTTGGAGAA 
GGCACAGTGA CACGCATCAC TCCTAAAGGC AAAATCACAG TCCAGTTCTG TGATATGCGA 
ATATGTAGAA TTTGTCCATT GAGTCAGCTA AAACCGCTTC CAGTTATGGC ATTTAATGTG 
AACAACTTAC CATTCACGGA ATCTATGCTC TCTGTCTGGG CTCAATTGGT CAACTTGGCA 
GGGAGCAAAT TAGAAAAACA CAGAATTAAG AAATCTACTA ATCAAGCCCT CACAGGACAA 
GTAGACTTGG ATTTGCTAAG ATGCCAACAG TTGAAATTAT ACATATTGAA AGCAGGTCGA 
GCATTGCTTT CCCATCAGGA TAAACTGAGG CAGATCTTAT CTCAACCAGC TTTACAAGAG 
AATGGAGTTC TCTCTACAGA TGATGGTACA GCATCCCCTG ATATTGGAGA TATGTCTCCT 
GAAGGACCTC AACCTCCAAT GATCCTTTTA CAGCAATTGT TGACATCTGC CACACAGCCA 
TCACCTGTAA AAGCAATATT TGACAAACAG GAACTTGAGG CTGCTGCTTT GGCTGTATGC 
CAGTGTCTAG CTGTGGAGTC AACTCATCCA TCAACTCCAG TGTTTGAAGA ATGTAGCTCT 
AGTGAGGCCA CAACACCTGT CACAGTCCAG CATATTCGAT CTGTAAAAGT GAAAAAGCGA 
AAGCAGTCTC CTCTCCCTGC TTTGCCTATT GTAGTACAGC TCATGGAAAT GGGATTTCCG 
AGGAAAAACA TTGAATTTGC ATTGAAGTCT CTTACTGGGA GTGCTTCAGG GTTACCAGGT 
GTTGAAGCCT TGGTTGGTTG GTTGCTGGAT CATCCTGATG TCCAAATTAC TGATCTCTCA 
GATGCTGATA CAGTCTCTGA TGAATACTCT GATGATGAAA TAGTGGAAGA TGTGGAAGAG 
ACTGAAGCAT CTTACCCTGT GTCTAGTTGT GCTGTTGTAA CGGAGAGTCA CACTTATAAA 
AAACGAGCTG ATTTCTTAAG TAATGATGAC TATGCAGTAT ATGTTAGAGA AAACATTCAG 
GTAGGAATGA TGGTTAGGTG CTGCAGAACG TATGAAGAAG TTTGTGAAGG AGATGTAGGT 
AAAGTGATTA AATTGGACAG AGATGGATTA CACGACCTCA ATGTGCAGTG TGATTGGCAA 
CAAAAGGGGA GTACCTATTG GGTTAGGTAT ATCCATGTTG AACTTATAGG TTTCCCACCA 
CAGAGTTCTC CTTCCCATAT CAAGATTGGT GATAAAGTTA GGGTGAAAAC ATCAGTAACC 
ACACCAAAAT ATAAATGGGG ATCAGTTACT CATAGAAGTG TGGGAGTTGT GAAAGCTTTT 
AGTGCCAATG GAAAAGATGT TATTGTGGAT TTTCCTCAGC AGTCTCATTG GACAGGATTA 
TTGTCAGAAA TGGAATTGGT GCCTAGTATT CATCCTGGAG TTACGTGTGA TGGCTGCCAA 
ATGTTTCCTA TCAATGGTCC TCGTTTCAAA TGCAGGAGTT GTGATGATTT TGACTTTTGT 
GAGACCTGTT TTAAAACCAA GAAGCATAAT ACCAGACATA GTTTTGGCAG AATAAATGAA 
CCAGGTCAGT CTCTTGTATT TTGTGGGCGT TCTGGGAAAC AGCTAAAACG TTGCCACAGT 
AGTCAGCCTG GAATGCTCTT GGATAATTGG TCAAGAATTG TTAAGAATTT AAATGTGTCC 
TCATCAGTAA ATCAGGCTTC TCGCTTGATT GATGGCAGTG AACCATGTTG GCAGTCATCT 
GGATCACAAG GAAAGCATTG GATTCGACTG GAGATTTTCC CCGATGTTCT TGTTCATAGA 
CTGAAAATGA TAGTAGATCC TGCTGACAGT AGCTACATGC CTTCACTAGT TGTAGTTTCA 
GGTGGGAATT CCCTAAATAA TTTAATTGAA CTGAAGACAA TCAATATTAA CCCAGCTGAT 
ACCACAGTGC CTCTTCTAAA TGACTGCACA GAGTATCACA GGTATATTGA GGTTGCCATA 
AAGCAATGCA GAAGCTCAGG AATAGATTGT AAAATTCATG GTCTTATTAT ACTGGGACGA 
ATTCGGGCAG AAGATGAAGA TTTGGCTGCT GTTCCTTTCT TAGCGTCAGA TAATGAAGAA 
GAAGATGATG AAAAAGCTAA CACTGGGAGT CTTATTAGGA AGAAGGCTTC TGGATTGGAA 
TCAGCAGCCA CAATAAGGAC CAAAGTTTTT GTCTGGGGGC TGAATGACAA GGATCAATTA 
GGAGGATTAA AGGGTTCTAA GATAAAAGTT CCTTCATTCT CTGAAACCCT TTCTGCTTTG 
AATGTAGTTC AAGTGGCAGG TGGATCTAAA AGTTTGTTTG CAGTGACTGT GGAGGGTAAA 
GTGTATGCTT GTGGAGAAGC TACTAATGGA CGACTGGGTT TAGGTATTTC TAGTGGAACT 
GTACCCATTC CGCGGCAGAT TACAGCTCTC AGCAATTATG TGGTAAAGAA AGTTGCTGTT 
CACTCAGGTG GCCGGCATGC TATGGCTTTA ACTGTTGATG GAAAGATATT TTCATGGGGT 
GAAGGTGATG ATGGAAAACT AGGACACTTC AGCAGAATGA ACTGTGATAA ACCAAGACTG 
ATAGAGGCAT TGAAAACCAA ACGTATTCGT GATATAGCTT GTGGTAGTTC TCACAGTGCT 
GCCATTACAT CAAGTGGTGA ATTGTATACA TGGGGCCTTG GAGAATATGG CCGGTTGGGA 
CATGGGGATA ATACTACACA GCTGAAACCC AAAATGGTGA AAGTTCTTCT TGGTCATCGA 
GTTATTCAAG TTGCTTGTGG AAGTAGAGAT GCCCAGACTC TGGCTTTGAC AGATGAAGGT 
TTGGTCTTTT CCTGGGGAGA TGGTGACTTT GGGAAACTGG GCCGGGGAGG AAGTGAAGGA 
TGTAACATTC CTCAGAACAT TGAGAGACTT AATGGACAAG GAGTTTGCCA GATTGAGTGT 
GGGGCTCAAT TTTCCTTGGC ACTTACAAAG TCAGGAGTAG TATGGACTTG GGGTAAAGGA 
GATTACTTTA GGCTTGGTCA TGGCACTGAT GTTCATGTGA GAAAACCACA AGTAGTAGAA 
GGACTACGAG GAAAGAAGAT AGTTCATGTG GCAGTAGGCG CTCTTCACTG CTTAGCTGTC 
ACTGATACAG GTCAAGTGTA TGCTTGGGGT GACAATGATC ATGGACAACA AGGCAATGGG 
ACTACTACTG TCAACAGAAA GCCTACCCTT GTACAAGGCT TAGAAGGACA AAAAATCACT 
CGGGTAGCTT GTGGCTCATC TCATAGTGTG GCGTGGACAA CAGTGGATGT AGCAACCCCA 
TCTGTTCATG AACCTGTCCT CTTTCAAACT GCTAGGGACC CTTTAGGCGC TTCTTACTTA 
GGTGTTCCTT CCGATGCTGA TTCTTCTGCT GCCAGTAACA AAATCAGTGG TGTAAATAGT 
TCCAAGCCTA ACCGCCCTTC CCTTGCTAAG ATTCTCTTGT CTTTGGATGG AAACCTTGCC 
AAACAGCAAG CATTATCTCA TATACTTACA GCATTGCAAA TTATGTATGC GAGAGATGCA 
GTAGTTGGAG CATTAATGCC TGCTAGTATG ATCGCACCAG TGGAATGCTC ATCTTCTTCA 
TCACCTGCAC CCCCCTCAGA TGTGTCTTCA GCAGGAAGTC CCATGAATGG TGAAGAGTCT 
GTGTTAGCTG TGGATGTAGA AGATAGACTA AGCCCAAATC CATGGCAAGA CAAGAGAGGG 
GAGGTAGTTT CTTCAGAAGA TGCTGTGACT CCATCAGCAG TGACTCCCTC AGCTTCCTCT 
GCCTCTTCTC GACCATTTAT TCCAGTAACT GATGATCCTG GAGCTGCCAG TATCATTGCA 
GAAACCATGA CTAAAACCAA AGAAGATGTT GAAAGCCAAA ATAAAGTATC AGGTCCGGAA 
CCTCAATATT TGGATGAATT TACTAGTCTC TTAGTGCCAG ATGATACCCG TGTCATGGTT 
GACTTGCTCA AGCTGTCTGT TTCTAATCGA GCTGGTGAAA AGGGAAAAGA TGTTCTTTCA 
GCTGTGCTTT CTGGTATGGG CACTGCTTAT CCACAGGTAG CTGATATGTT GTTGGAGCTC 
TGTGTTACAG AGTTAGAAGA TGTTGCCACA GACTCTCAGA GTGGTCGACT CTCTTCCCAA 
CCTGTTGTAG TAGAAAGTAG TCATCCGTAT ACTGATGATA CCTCTACCAG TGGTACTGTC 
AAAATACCAG GTGCTGAAGG ACTCAGAGTA GAGTTTGATC GACAATGTTC TACAGAACGG 
CGTCATGACC CTCTCACAAT CATGGATGGT GTCAATAGGA TAGTTTCAGT TCGATCAGGT 
CGAGAATGGT CTGATTGGTC CAGTGAACTT CGAATTCCTG GAGATGAATT AAAGTGGAAG 
TTCATCAGTG ATGGATCAGT GAATGGATGG GGTTGGCGAT TCACTGTCTA TCCTATTATG 
CCAGCTGCAG GCCCTAAAGA TCTTCTTTCA GATCGGTGTA TTCTTTCCTG TCCCTCAATG 
GACTTGGTTA CATGCTTGTT GGACTTTAGA TTAAATTTAG CTTCCAATAG AAGTATTGTT 
CCTCGTTTGG CAGCATCACT TGCAGCATGT GCTCAATTGA GTGCTCTAGC TGCTGGTCAC 
AGAATGTGGG CCCTTCAAAG ACTGAGGAAA CTGCTTACAA CTGAATTTGG ACAATCAATC 
AATATAAATA GGATACTTGG AGATAATGAT GGAGAAACAA GAACCATGAG TTTTACCGGT 
AGTGCTTTAG CAGCTTTGGT AAAAGGTCTT CCAGAAGCTT TGCAAAGACA GTTTGAATAT 
GAAGATCCGA TTGTTAGAGG AGGCAAACAA CTGCTCCATA GCCCTTTCTT TAAGGTGCTG 
GTAGCTCTTG CTTGTGACCT AGAACTGGAT ACTCTTCCTT GCTGTGCTGA GACACACAAA 
TGGGCTTGGT TTAGAAGATA CTGTATGGCC TCTAGAGTTT CAGTAGCCTT GGACAAACGA 
ACACCATTAC CTCGCCTTTT CCTTGATGAG GTGGCAAAGA AAATCAGGGA GTTAATGGCC 
GACAGTGAAA ATCTGGATGT TCTGCATGAG AGCCATGACA TTTTTAAGAG AGAACAAGAT 
GAACAACTCG TCCAGTGGAT GAACAGGAGA CCTGATGACT GGACTCTTTC TGCTGGAGGC 
AGTGGAACAA TTTATGGTTG GGGTCACAAT CATCGAGGAC AGCTTGGCGG TATTGAGGGA 
GCAAAAGTCA AAGTTCCAAC ACCTTGTGAA GCTCTTGCAG CTCTGCGGCC CATACAGTTA 
ATTGGAGGGG AACAGACTCT CTTTGCGGTA ACTGCTGATG GGAAGCTTTA TGCTACAGGG 
TATGGTGCAG GTGGCAGATT GGGAATTGGA GGAACTGAAT CAGTTTCTAC TCCAACATTG 
CTTGAATCTA TTCAGCATGT ATTTATAAAA AAAGTTGCTG TGAACTCTGG AGGAAAACAC 
TGCTTAGCAC TCTCATCAGA AGGAGAAGTT TATTCTTGGG GTGAGGCAGA AGATGGAAAA 
TTGGGGCATG GAAACAGAAG CCCCTGTGAT CGCCCACGTG TAATTGAATC TTTGCGAGGA 
ATAGAAGTTG TTGATATTGC TGCAGGTGGA GCCCACAGTG CATGTATTAC AGCAGCTGGT 
GACCTCTATA CGTGGGGTAA AGGAAGATAT GGTCGCCTAG GTCATGGGGA TAGTGAAGAT 
CAGTTAAAAC CAAAACTGGT GGAAGCTCTC CAAGGCTATC GAGTTATTGA TATTGCCTGT 
GGCAGTGGAG ATGCACAGAC ACTTTGCCTT ACTGATGATG ATACTGTATG GTCCTGGGGT 
GATGGGGACT ATGGAAAACT TGGCAGAGGA GGCAGTGATG GCTGTAAAGT GCCAATGAAG 
ATTGATTCTC TCACAGGTCT TGGAGTGGTT AAAGTAGAGT GTGGATCACA ATTTTCTGTT 
GCACTTACCA AGTCTGGAGC AGTTTATACT TGGGGTAAAG GCGATTATCA CAGGTTGGGT 
CATGGATCAG ACGACCATGT TAGACGGCCT CGGCAAGTGC AAGGCCTACA AGGAAAGAAA 
GTAATAGCAA TTGCTACTGG TTCTTTACAC TGTGTGTGCT GCACTGAAGA CGGTGAAGTT 
TATACTTGGG GTGACAATGA TGAAGGGCAA CTTGGGGATG GAACAACTAA TGCAATCCAG 
AGACCTCGGT TGGTGGCAGC ACTTCAGGGT AAAAAGATAA ATAGGGTGGC TTGTGGTTCA 
GCACACACCT TAGCGTGGTC CACGAGTAAA CCTGCCAATG CTGGGAAGCT TCCAACACAG 
GTTCCTATGG AATACAACCA TTTACAGGAA TTTCTCATAA TTCCTTTACG AAACAGACTT 
TTGTTATTAC ATCATATCTC AGAACTCTTT TGTCCATGCA TTCCCATGTT TGACCTTGAG 
GGGCGTCTTG ATGAAACTGG CCAAGGACCT TCAGTGGGAT TTGACACATT ACGAGGAATT 
CTGATATCTC AGGGAAAGGA AGCAGCTTTC CGAAAAGTTG TACAAGCAAC TATGGTGAGA 
GATCGTCAAC ATGGTCCTGT AGTTGAATTA AACAGAATAC AGGTAAAACG ATCAAGAAGT 
AAGGGTGGTT TGGCTGGTCC TGATGGTACT AAATCTGTTT TTGGCCAAAT GTGTGCTAAA 
ATGATTTCAT TTAGTCCAGA CAGTCTTCTT CTACCTCATC GAGTATGGAA AGTCAAGTTT 
GTTGGCGAGT CTGTGGATGA TTGTGGAGGT GGTTATAGTG AATCAATAGC AGAAATGTGT 
GAAGAACTTC AGAATGGACT TACTCCTCTC CTAATTGTAA CTCCAAATGG CAGGGATGAA 
TCTGGAGCCA ATAGAGATTG CTTCTTATTG AACCCAGCTG CCAGATCTTC TTTGCATACC 
AATATGTTTC GTTTCTTGGG TGTATTGCTA GGCATTGCCA TTCGAACTGG GAGTCCATTA 
AGCCTCAACC TTGCAGAACC GGTTTGGAAG CAACTGGCAG GAATGAACCT TACAATTGCT 
GATCTCAGTG AGGTTGATAA GGATTTTATT CCTGGCCTGA TGTACATTCG GGACAATGAA 
GCTACTTCTG AGGAATTTGA AGCAATGAGC CTTCCCTTCA CAGTGCCAAG TGCAAGTGGG 
CAGGATATTC AATTGAGCTC TAAATATACC CACATCACAT TGGATAACCG TGCTGAGTAT 
GTTAGATTGG CAATAAATTA CAGACTCCAT GAATTTGATG AACAAGTTGC TGCAGTTCGG 
GAAGGAATGG CTCGAGTTGT CCCTGTTCCT CTTCTTTCAC TCTTTACTGG ATATGAACTG 
GAAACTATGG TGTGTGGAAG CCCCGATATT CCACTTCATC TTCTAAAATC TGTAGCCACA 
TATAAAGGAA TTGAACCTAC TGCTTCTTTG ATCCAGTGGT TCTGGGAAGT GATGGAATCT 
TTCTCTAATA CAGAGCGCTC TCTCTTTCTT CGTTTTGTGT GGGGCAGAAC ACGACTTCCT 
AGAACAATTG CTGATTTCAG AGGCAGAGAT TTTGTTATTC AGGTTTTGGA TAAGTACAAT 
CCACCTGACC ACTTCCTTCC AGAGTCATAT ACATGCTTCT TTTTGCTGAA GTTACCCAGG 
TATTCCTGTA AACAAGTACT AGAGGAAAAA CTGAAATATG CCATTCATTT TTGCAAGTCT 
ATAGACACTG ATGATTATGC CCGGATAGCA CTCACAGGAG AACCAGCTGC TGATGACAGC 
AGTGATGATT CAGATAATGA AGATGCAGAT TCATTTGCAT CGGACTCTAC TCAAGATTAT 
TTAACAGGAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC2 ( XM_031959091.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031814951.1 Sarcophilus harrisii HECT and RLD domain containing E3 ubiquitin protein ligase 2 (HERC2), transcript variant X4, mRNA.

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CloneID	OSa229208
Clone ID Related Accession (Same CDS sequence)	XM_031959091.1
Accession Version	XM_031959091.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	E3 ubiquitin-protein ligase HERC2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045428.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTCTG AATCCTTCTG TTTAGCTGCC CAGGCTAGAT TTGACTCCAA GTGGTTAAAA 
ACAGATTTAC AGCTTGCTTT TACAAGAGAT GGACTCTGTG GCCTATGGAA TGAAATGGTC 
AAAGATGGAG AAATTGTATA TCCTGGGACA GATGTAATAC AGAATGGAGA ACTACCTCCT 
CGAAAAGATG ACACCATGGA ATGTCAGAGT GGAACAAAGA AAGAAGATCA AAATGATAAA 
GAGAAGAAAG ATGAAGAGGA AATTCCAGTC CCTATGTATA AGGCCAAATC ACTTGTGGAG 
AGTTGGGTAT GGGGCAAACA ACCAGATGTG AACGAGTTGA AAGAGTGCCT TTATGTGCTA 
GTGAAAGAAC AGCAAACTCT TGCTACACGG ATGGCTACAA CAACTCTTTC AGCAGTAAGG 
CTAAAGCAGA GGCTGGTTAT ACTGGAACGA TATTTCATTG CACTGAATAG AACTGTTCTT 
CAGGAAAATG TCAAAGTTAA GTGGAAAAGC AACAGTATTC CTTTACCTAC TGTCGACAAA 
AAAAGTTCTA GACCTATAGG AAAAGGTGTA GAAGGACTTG CAAGAGTTGG ATCCAGAGCA 
GCCCTTTCAT TTGCTTTTGC ATTCCTACGC AGAGCTTGGC GGTCAGGGGA AGATGCCGAT 
CTATGCAGTG AATTGTTACA GGAGTCTCTT GATGCCTTGA GAGCCCTACC AGAAGCATCT 
CTTTTTGATG AAAGCACAGT GTCATCTGTT TGGTTGGAAG TTGTGGAGAG AGCAACTAAA 
TTCCTAAGGT CTGTTGTTAC TGGTGATGTT CATGGAACTG CAAGTACCAA AGGACCAGGA 
AACATTCCAT TACAAGACCA GCACTTAGCA CTTGCCATAT TGTTAGAGTT GGCTGTGCAG 
AGAGGCACTC TAAGCCAAAT GTTATCTGCC ATTATGTTAC TACTTCAATT ATGGGACAAT 
GGGACAAGGG AAACAGATAA TGAACGATCT GCCCAAGGAA CTAGTGCACC ACTTCTCCCT 
TTATTACAAA GATTTCAGAG TATAATCTGC AATAAAGACA TACTTAATAC TGAAGATGAG 
ATTCAGATGC TGTCTGGTCT TCTGAGTCCC AATGAAAGTT TTTTGAGGTA CCTTACTTTA 
CCACAAGACA ATGAATTAGC CATTGATTTG CGTCAAACTG CAGTAGTGAT TATGGCGCAT 
TTAGATCGTC TGGCCACTCC CTGTATGCCT CCTCAGTGTA GTTCTCCAAC ATCTCATAAG 
GGATCTTTAC AAGAAGTCAT AGGTTGGGGC TTAATAGGAT GGAAATACTA TGCTAATGTG 
ATTGGCCCAA TTCAATGTGA AGGACTTGCA AATCTTGGGA TCACACAGAT TGCCTGTGCA 
GAAAAGCGTT TCCTGATTTT GTCTCGGAAT GGGCGAGTTT ACATGCAGGC TTACAACAGT 
GACACATTGG GACCCCAACT AGTTCAAGGT CTTGCCTCTA GAAATATTGT GAAAATTGCA 
GCACATTCAG ATGGTCATCA TTATCTGGCT TTGGCAGCCA CTGGAGAGGT CTTTTCTTGG 
GGCTGTGGAG ATGGTGGACG GTTAGGCCAT GGTGATACAG TGCCTCTAGA AGAACCTAAG 
ATGATATCTG CTCTCTCAGG AAAGCAAGCT GGGAAACATG TAGTTCATAT CGCATGTGGA 
AGTACTTATA GTGCAGCTAT TACAGCTGAA GGAGAGTTAT ATACTTGGGG TCGAGGAAAC 
TATGGCAGGC TGGGACATGG CTCAAGTGAA GATCAGACAA TTCCAATGCT AGTAACTGGA 
CTTAAAGGAC TGAAGGTTAT TGATGTCTCT TGTGGGAGTG GGGATGCTCA GACTCTTGCA 
GTGACTGAGA ATGGTCAAGT CTGGTCTTGG GGCGATGGTG ATTATGGAAA ACTAGGAAGA 
GGTGGTAGTG ATGGTTGTAA AACACCAAAA CTGATTGAAA AACTTCAAGA CTTAGATGTT 
GTTAAAGTAC GTTGTGGAAG CCAATTTTCC GTTGCTTTAA CAAAAGATGG TCAAGTTTAC 
TCGTGGGGAA AAGGTGACAA TCAGAGACTT GGGCATGGAA CAGAGGAGCA TGTTCGATAT 
CCGAAACTGT TAGAAGGCTT ACAAGGTAAA AAAGTCGTTG ATGTGGCTGC TGGTTCTACA 
CACTGCTTAG CACTTACTGA GGATAGCGAA GTTCATAGCT GGGGGAGTAA TGATCAGTGT 
CAGCACTTTG ACACCCTTCG GATTACCAAA CCTGAACCTA CTGCATTGCC AGGATTAGAT 
ACAAAACATA TAGTTGGAAT TGCTTGTGGG CCCGCTCAGA GTTTTGCTTG GTCTTCTTGT 
TCTGAGTGGT CCATTGGCCT ACGTGTACCT TTTGTGGTGG ATGTTTGTCC CATGACATTT 
GAACAGTTGG ATCTCTTACT ACGACAAGTA AGTGAGGGAA TGGATGGCAC CTCTGACTGG 
CCACCACCAC AGGAGAAAGA ATGTATGGCA GTGGCAACAT TGAACCTTCT GAGACTTCAG 
TTGCATGCTG CTATTAGTCA CCAGGTTGAT CCGGAATGTC TTGGATTGGG TCTGGGAAGT 
GTACTCTTGA ATAGCTTGAA GCAGACAGTT GTAACACTAG CAAGTAATGC TGGAGTTCTT 
AATACAGTGC AATCAGCTGC TCAAGCAGTA CTGCAGAGTG GATGGTCTGT GCTGCTTCCA 
ACTGCAGAGG AACGAGCAAG GGCTCTTTCA GCTCTCTTAC CCAGTGCAGT TTCAGGAAAT 
GACATGAATG TAAGTCCAGG ACGTCGTTTT ATGATTGATC TTCTTGTAGG CAGTTTGATG 
GCTGATGGAG GATTAGAATC AGCATTAAAT GCTGCCATCA CTGCAGAAAT CCAGGATATT 
GAAGCAAAAA AAGAAGCTCA AAAGGAAAAA GAGATTGATG AGCAGGAAGC AAATGCTTCA 
ACACTTCATC GGAGTAGGAC ACCATTAGAT AAAGACCTCA TTAATACTGG AATTTATGAA 
TCTGCCGGAA AACAGAGTTT GCCTCTGGTT CAATTAATAC AACAGCTTCT TAGAAATATT 
GCTTCCCAGA CAATAGCCAA ATTGAAAGAT GTTGCTCGTC GAATTTTGTC CTGTATAGAA 
CATGATCAAT ATAGTCAAGA CAGATCTGCC TCCCTGGATT TGTTGCTTCG TTTTCAGCGT 
CTGCTGGTCA GTAAACTCTA TCCAGGAGAC AGTGGTGGTC AAGTTTCAAA TTCCTTTAGT 
CCTGAGCTAT TAGGAGTTGG TTCTTTGCTG AAAAAATATA CAGCTCTTTT GTGCACACAC 
ATTGGAGATA TATTGCCTGT GGCAACTAGT ATTGCATCTT CCAGTTATCG GCATTTTGCA 
GAAGTATCTC GTGTTGTGGA TGGTGATCTA ACAGGTGTTC TTCTTCCAGA GTTGATAGTA 
TCTATTGTCC TTTTGCTCAA TAAAAGTCCT GGATTAATGC AGGAAGCTGG GGCAATTCCT 
CTCTTAGCTG GTCTACTGGA ACATCTAGAT AGATTTAACC ATTTGGCACC AGGAAAAGAG 
AGAGATGATA ATGAGGATTT AGCCTGGCCT GGAATAATGG GATCATTTTT TACAGGTCAG 
AATAGTAGAA ATAATGAGGA AGTGACGCTC ATACGTAAAG CTGATCTGGA GAATCACAAT 
AAAGATGGAG GCTTCTGGAC TGTGATTGAC GGGAAAGTGT ATGATATAAA AGACTTTCAG 
ACACAGTCTT TAACAGGCAA TAGCATTCTT GCTCAGTTTG CAGGTGATGA CCCGGTGGTT 
GCTTTGGAAG CTGCTTTACA GTTTGAGGAC ACAAGGGATT CAATGCATGC ATTTTGTGTT 
GGCCAGTATA TGGAGCCTGA CCAGGAAGTG ATTACAACGC CAGACCTTAC CAGTCTCTCT 
TCACCTCTCA TAGATCCAGA AAGAAATCTG GGTCTCCTCC TTGGACTTCA TGCTTCCTAT 
TTAGCAATGA GCACACCACT ATCTCCTGTG GAGATTGAAT GTGCCAAATG GCTTAAGTCA 
TCCATATTCT CTGGAGGATT GCAAACCAGT CAGATACATT ATAGTTACAA TGAAGAGAAA 
GATGAAGACC ACTGCAGTTC TCCTGGAAAT ACTACTCCAA ACAAATCCAA ACTCTATTCC 
CATAGATTGA CTTTGAGTGA TCATTCCCAA CCATTTTTGC AAGCCATTGC AGATAATAAC 
ATTCAGGATC ATACCGTGAA GGATTTTTTA TGTCAGATAG AAAGGTATTG CAAGCAATGC 
CATTTGACCA CACCGATAAC ATTTCCTCCT GAACATCCTG TGGAAGAAGT GGGACGTTTA 
CTCTTATGTT GTCTTCTAAA GCATGAAGAT TTAGGTCATG TGGCCTTATC TTTAGTTCAC 
CCAGTTACAC TTGGTGTTGA CCAAGTCAAA CACAGAACGT TGCCTAAGTC TGTAGTGGAT 
GTTTGTAGAG TCGTCTACCA GGCAAAATGC TCACTAATAA AGACACATCA GGAACAGGGA 
CGTTCTTATA AGGAAGTGTG TGCTCCTGTT ATTGAACGTT TGCGATTTCT TTTTAATGAA 
TTGCGTCCTG CAGTCTGCAA TGACCTCTCA ATAATGTCCA AACTTAAACT TTTAAGCTCT 
TTGCCTCGTT GGAGAAGAAT AGCTCAGAAG ATGATACGAG AGAGAAGGAA ATTAAAAAGA 
GTACCCAAAA AGTCAGAACC TACTGATGTG GAAGAATCTA AGATTGGCAA TGAAGAGGGT 
GACTTAGAAG AAGCCTGTAT TCTTCCTCAT AGTCCTGTAT CTGTAGATAA AAGACCTGTT 
CCAGTTAAAT CACCTAAGGA TAAATGGCAG CCACTTCTGA GTACAGTTAC AGGTGTTCAC 
AAATACAAAT GGCTTAAACA GAATGTTCAA GGCCTTTATC CTCAGTCTGC ACTGCTTAGC 
ACAATTGTTG AATTTGCGCT TAAAGAAGAG CCAGTTGATG TGGAAAAGAT GAGAAAGTGC 
TTACTCAAAC AGTTGGAGAG AGCAGAAGTT CGTTTGGAAG GAATAGACAC GATTTTAAAA 
TTGGCAAATA AAAATTTCTT GCTTTCGTCT GTGCAATATG CTATGTTCTG TGGATGGCAA 
AGACTTATCC CAGAAGGAAC CAATATAGGG GAACCACTTA CAGATTGTTT AAAGGATGTT 
GATCTGATTC CACCATTTAA TCGAATGCTA CTAGAAGTAA CTTTTGGAAA GTTGTATGGT 
TGGGCCATTC AGAATATTCG AAATGTTTTG TTGGATGCAA ATGCCAGATT TAAAGAGCTA 
GGTATCCAGC CTGTTCCTCT GCAAACTATT ACTAATGAGA ATCCAGCAGG GCCAAGCCTT 
GGAACCATCC CACAAGCACG TTTTCTCCTG GTTATGCTCA ACATGCTTAC CCTTCAATAT 
GGTGCAAACA ACTTGAATCT TCTTTTGAAC TCTGGCATGC TAGCATTGAC TCAGACAACA 
TTGCGATTAA TAGGTCCTAG TTCTGATAAT ATCGAGGAAG AAATGAATGC CTCTGCACGA 
GGAGCTTCAG CTACTGTTTT GGAAGAATCC AGAAAAGAAA CCACTCCAGT ACAACTTCCT 
GTTTCAGGGC CAGAACTGGC AGCAATGATG AAAATTGGTA CCAGGGTCAT GAGGGGAGTG 
GACTGGAAAT GGGGAGATCA GGATGGTCCA CCTCCAGGTT TAGGTCGTGT GATTGGTGAA 
TTGGGAGAGG ATGGATGGAT AAGGGTTCAG TGGGATACAG GCAGTACTAA TTCATACCGA 
ATGGGAAAGG AAGGAAAGTA TGATCTTAAA TTGGCTGAGC CTCCACCTGC AGCTCAGCCC 
TCAACAGAGG ATTCGGACAC TGAGGATGAT TCTGAAAGTG AACAAATGGA AAGAAACTTC 
CACCCTACTT CTATTATGCT TACAAGTACT GTTAATTTAC TCCAAACTCT CTGTCTCTGT 
GCTGGAATTC ATGCAGAAAT CATGCAAAAT GAAGCTACAA GAACTCTGTG TGGTCTACTT 
AGAATGTTAG TGGAAAGTGG AACAGTAGAT AAAACATCTT CTTTACCAAA TAAGATGGTT 
TATAAAGAGC AGCACAGAAG TTGGTGTACA CTTGGATTTA TTCGAAGTAT AGCACTTATG 
CCTCAAATGT GTAGCACCCT TAGCACATCT CAGTGGATCA CATTACTAAT GAAAATTGTG 
GAAGGACACG AGTCATTTAC AGCAGCTTCG CTTCAGAGAC AGATCTTAGC AGTACATTTA 
CTACAAGCTG TACTTCCATC TTGGGACAAA ACAGAAAGAT CAAGAGATAT GAAGTTTCTT 
GTGGAAAAAC TTTTTGGCTT CTTGGGCAGT CTACTTAGTA CATGCTCTTC AGACATACCT 
TTGCTCAGAG AATCAACTCT GAGAAGGAGG AAGTCCAGAC CCCAGGCATC TTTGACTGCA 
ACCCATAGTA GTACTTTGGC AGAGGAGATA GTGGCCCTGC TAAGGACTCT ACATTCACTG 
AGCCAGTGGA ATGGACTCAT AAACAAGTAC ATCAACTCTC AGCTAAACTC CATCACCCAC 
ATCTTTGCAG GAAAACAGTC TGAAGGGACT GTGTTAGAAG ATTACTTTCC TGATTCTGAG 
AACCCTGAAG TTGATGGCCT AATGGCAGTG CTAGCTGTAA TTGGTGGAAT AGATAGTCGA 
TTGAGGCTGG GTGGCCAAGT GGTACATGAT GAATTTGGAG AAGGCACAGT GACACGCATC 
ACTCCTAAAG GCAAAATCAC AGTCCAGTTC TGTGATATGC GAATATGTAG AATTTGTCCA 
TTGAGTCAGC TAAAACCGCT TCCAGTTATG GCATTTAATG TGAACAACTT ACCATTCACG 
GAATCTATGC TCTCTGTCTG GGCTCAATTG GTCAACTTGG CAGGGAGCAA ATTAGAAAAA 
CACAGAATTA AGAAATCTAC TAATCAAGCC CTCACAGGAC AAGTAGACTT GGATTTGCTA 
AGATGCCAAC AGTTGAAATT ATACATATTG AAAGCAGGTC GAGCATTGCT TTCCCATCAG 
GATAAACTGA GGCAGATCTT ATCTCAACCA GCTTTACAAG AGAATGGAGT TCTCTCTACA 
GATGATGGTA CAGCATCCCC TGATATTGGA GATATGTCTC CTGAAGGACC TCAACCTCCA 
ATGATCCTTT TACAGCAATT GTTGACATCT GCCACACAGC CATCACCTGT AAAAGCAATA 
TTTGACAAAC AGGAACTTGA GGCTGCTGCT TTGGCTGTAT GCCAGTGTCT AGCTGTGGAG 
TCAACTCATC CATCAACTCC AGTGTTTGAA GAATGTAGCT CTAGTGAGGC CACAACACCT 
GTCACAGTCC AGCATATTCG ATCTGTAAAA GTGAAAAAGC GAAAGCAGTC TCCTCTCCCT 
GCTTTGCCTA TTGTAGTACA GCTCATGGAA ATGGGATTTC CGAGGAAAAA CATTGAATTT 
GCATTGAAGT CTCTTACTGG GAGTGCTTCA GGGTTACCAG GTGTTGAAGC CTTGGTTGGT 
TGGTTGCTGG ATCATCCTGA TGTCCAAATT ACTGATCTCT CAGATGCTGA TACAGTCTCT 
GATGAATACT CTGATGATGA AATAGTGGAA GATGTGGAAG AGACTGAAGC ATCTTACCCT 
GTGTCTAGTT GTGCTGTTGT AACGGAGAGT CACACTTATA AAAAACGAGC TGATTTCTTA 
AGTAATGATG ACTATGCAGT ATATGTTAGA GAAAACATTC AGGTAGGAAT GATGGTTAGG 
TGCTGCAGAA CGTATGAAGA AGTTTGTGAA GGAGATGTAG GTAAAGTGAT TAAATTGGAC 
AGAGATGGAT TACACGACCT CAATGTGCAG TGTGATTGGC AACAAAAGGG GAGTACCTAT 
TGGGTTAGGT ATATCCATGT TGAACTTATA GGTTTCCCAC CACAGAGTTC TCCTTCCCAT 
ATCAAGATTG GTGATAAAGT TAGGGTGAAA ACATCAGTAA CCACACCAAA ATATAAATGG 
GGATCAGTTA CTCATAGAAG TGTGGGAGTT GTGAAAGCTT TTAGTGCCAA TGGAAAAGAT 
GTTATTGTGG ATTTTCCTCA GCAGTCTCAT TGGACAGGAT TATTGTCAGA AATGGAATTG 
GTGCCTAGTA TTCATCCTGG AGTTACGTGT GATGGCTGCC AAATGTTTCC TATCAATGGT 
CCTCGTTTCA AATGCAGGAG TTGTGATGAT TTTGACTTTT GTGAGACCTG TTTTAAAACC 
AAGAAGCATA ATACCAGACA TAGTTTTGGC AGAATAAATG AACCAGGTCA GTCTCTTGTA 
TTTTGTGGGC GTTCTGGGAA ACAGCTAAAA CGTTGCCACA GTAGTCAGCC TGGAATGCTC 
TTGGATAATT GGTCAAGAAT TGTTAAGAAT TTAAATGTGT CCTCATCAGT AAATCAGGCT 
TCTCGCTTGA TTGATGGCAG TGAACCATGT TGGCAGTCAT CTGGATCACA AGGAAAGCAT 
TGGATTCGAC TGGAGATTTT CCCCGATGTT CTTGTTCATA GACTGAAAAT GATAGTAGAT 
CCTGCTGACA GTAGCTACAT GCCTTCACTA GTTGTAGTTT CAGGTGGGAA TTCCCTAAAT 
AATTTAATTG AACTGAAGAC AATCAATATT AACCCAGCTG ATACCACAGT GCCTCTTCTA 
AATGACTGCA CAGAGTATCA CAGGTATATT GAGGTTGCCA TAAAGCAATG CAGAAGCTCA 
GGAATAGATT GTAAAATTCA TGGTCTTATT ATACTGGGAC GAATTCGGGC AGAAGATGAA 
GATTTGGCTG CTGTTCCTTT CTTAGCGTCA GATAATGAAG AAGAAGATGA TGAAAAAGCT 
AACACTGGGA GTCTTATTAG GAAGAAGGCT TCTGGATTGG AATCAGCAGC CACAATAAGG 
ACCAAAGTTT TTGTCTGGGG GCTGAATGAC AAGGATCAAT TAGGAGGATT AAAGGGTTCT 
AAGATAAAAG TTCCTTCATT CTCTGAAACC CTTTCTGCTT TGAATGTAGT TCAAGTGGCA 
GGTGGATCTA AAAGTTTGTT TGCAGTGACT GTGGAGGGTA AAGTGTATGC TTGTGGAGAA 
GCTACTAATG GACGACTGGG TTTAGGTATT TCTAGTGGAA CTGTACCCAT TCCGCGGCAG 
ATTACAGCTC TCAGCAATTA TGTGGTAAAG AAAGTTGCTG TTCACTCAGG TGGCCGGCAT 
GCTATGGCTT TAACTGTTGA TGGAAAGATA TTTTCATGGG GTGAAGGTGA TGATGGAAAA 
CTAGGACACT TCAGCAGAAT GAACTGTGAT AAACCAAGAC TGATAGAGGC ATTGAAAACC 
AAACGTATTC GTGATATAGC TTGTGGTAGT TCTCACAGTG CTGCCATTAC ATCAAGTGGT 
GAATTGTATA CATGGGGCCT TGGAGAATAT GGCCGGTTGG GACATGGGGA TAATACTACA 
CAGCTGAAAC CCAAAATGGT GAAAGTTCTT CTTGGTCATC GAGTTATTCA AGTTGCTTGT 
GGAAGTAGAG ATGCCCAGAC TCTGGCTTTG ACAGATGAAG GTTTGGTCTT TTCCTGGGGA 
GATGGTGACT TTGGGAAACT GGGCCGGGGA GGAAGTGAAG GATGTAACAT TCCTCAGAAC 
ATTGAGAGAC TTAATGGACA AGGAGTTTGC CAGATTGAGT GTGGGGCTCA ATTTTCCTTG 
GCACTTACAA AGTCAGGAGT AGTATGGACT TGGGGTAAAG GAGATTACTT TAGGCTTGGT 
CATGGCACTG ATGTTCATGT GAGAAAACCA CAAGTAGTAG AAGGACTACG AGGAAAGAAG 
ATAGTTCATG TGGCAGTAGG CGCTCTTCAC TGCTTAGCTG TCACTGATAC AGGTCAAGTG 
TATGCTTGGG GTGACAATGA TCATGGACAA CAAGGCAATG GGACTACTAC TGTCAACAGA 
AAGCCTACCC TTGTACAAGG CTTAGAAGGA CAAAAAATCA CTCGGGTAGC TTGTGGCTCA 
TCTCATAGTG TGGCGTGGAC AACAGTGGAT GTAGCAACCC CATCTGTTCA TGAACCTGTC 
CTCTTTCAAA CTGCTAGGGA CCCTTTAGGC GCTTCTTACT TAGGTGTTCC TTCCGATGCT 
GATTCTTCTG CTGCCAGTAA CAAAATCAGT GGTGTAAATA GTTCCAAGCC TAACCGCCCT 
TCCCTTGCTA AGATTCTCTT GTCTTTGGAT GGAAACCTTG CCAAACAGCA AGCATTATCT 
CATATACTTA CAGCATTGCA AATTATGTAT GCGAGAGATG CAGTAGTTGG AGCATTAATG 
CCTGCTAGTA TGATCGCACC AGTGGAATGC TCATCTTCTT CATCACCTGC ACCCCCCTCA 
GATGTGTCTT CAGCAGGAAG TCCCATGAAT GGTGAAGAGT CTGTGTTAGC TGTGGATGTA 
GAAGATAGAC TAAGCCCAAA TCCATGGCAA GACAAGAGAG GGGAGGTAGT TTCTTCAGAA 
GATGCTGTGA CTCCATCAGC AGTGACTCCC TCAGCTTCCT CTGCCTCTTC TCGACCATTT 
ATTCCAGTAA CTGATGATCC TGGAGCTGCC AGTATCATTG CAGAAACCAT GACTAAAACC 
AAAGAAGATG TTGAAAGCCA AAATAAAGTA TCAGGTCCGG AACCTCAATA TTTGGATGAA 
TTTACTAGTC TCTTAGTGCC AGATGATACC CGTGTCATGG TTGACTTGCT CAAGCTGTCT 
GTTTCTAATC GAGCTGGTGA AAAGGGAAAA GATGTTCTTT CAGCTGTGCT TTCTGGTATG 
GGCACTGCTT ATCCACAGGT AGCTGATATG TTGTTGGAGC TCTGTGTTAC AGAGTTAGAA 
GATGTTGCCA CAGACTCTCA GAGTGGTCGA CTCTCTTCCC AACCTGTTGT AGTAGAAAGT 
AGTCATCCGT ATACTGATGA TACCTCTACC AGTGGTACTG TCAAAATACC AGGTGCTGAA 
GGACTCAGAG TAGAGTTTGA TCGACAATGT TCTACAGAAC GGCGTCATGA CCCTCTCACA 
ATCATGGATG GTGTCAATAG GATAGTTTCA GTTCGATCAG GTCGAGAATG GTCTGATTGG 
TCCAGTGAAC TTCGAATTCC TGGAGATGAA TTAAAGTGGA AGTTCATCAG TGATGGATCA 
GTGAATGGAT GGGGTTGGCG ATTCACTGTC TATCCTATTA TGCCAGCTGC AGGCCCTAAA 
GATCTTCTTT CAGATCGGTG TATTCTTTCC TGTCCCTCAA TGGACTTGGT TACATGCTTG 
TTGGACTTTA GATTAAATTT AGCTTCCAAT AGAAGTATTG TTCCTCGTTT GGCAGCATCA 
CTTGCAGCAT GTGCTCAATT GAGTGCTCTA GCTGCTGGTC ACAGAATGTG GGCCCTTCAA 
AGACTGAGGA AACTGCTTAC AACTGAATTT GGACAATCAA TCAATATAAA TAGGATACTT 
GGAGATAATG ATGGAGAAAC AAGAACCATG AGTTTTACCG GTAGTGCTTT AGCAGCTTTG 
GTAAAAGGTC TTCCAGAAGC TTTGCAAAGA CAGTTTGAAT ATGAAGATCC GATTGTTAGA 
GGAGGCAAAC AACTGCTCCA TAGCCCTTTC TTTAAGGTGC TGGTAGCTCT TGCTTGTGAC 
CTAGAACTGG ATACTCTTCC TTGCTGTGCT GAGACACACA AATGGGCTTG GTTTAGAAGA 
TACTGTATGG CCTCTAGAGT TTCAGTAGCC TTGGACAAAC GAACACCATT ACCTCGCCTT 
TTCCTTGATG AGGTGGCAAA GAAAATCAGG GAGTTAATGG CCGACAGTGA AAATCTGGAT 
GTTCTGCATG AGAGCCATGA CATTTTTAAG AGAGAACAAG ATGAACAACT CGTCCAGTGG 
ATGAACAGTG ATGTATCCAT TGTAGGAGAC CTGATGACTG GACTCTTTCT GCTGGAGGCA 
GTGGAACAAT TTATGGTTGG GGTCACAATC ATCGAGGACA GCTTGGCGGT ATTGAGGGAG 
CAAAAGTCAA AGTTCCAACA CCTTGTGAAG CTCTTGCAGC TCTGCGGCCC ATACAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031814951.1
CDS	258..12257
Translation	MSSESFCLAAQARFDSKWLKTDLQLAFTRDGLCGLWNEMVKDGEIVYPGTDVIQNGELPPRKDDTMECQSGTKKEDQNDKEKKDEEEIPVPMYKAKSLVESWVWGKQPDVNELKECLYVLVKEQQTLATRMATTTLSAVRLKQRLVILERYFIALNRTVLQENVKVKWKSNSIPLPTVDKKSSRPIGKGVEGLARVGSRAALSFAFAFLRRAWRSGEDADLCSELLQESLDALRALPEASLFDESTVSSVWLEVVERATKFLRSVVTGDVHGTASTKGPGNIPLQDQHLALAILLELAVQRGTLSQMLSAIMLLLQLWDNGTRETDNERSAQGTSAPLLPLLQRFQSIICNKDILNTEDEIQMLSGLLSPNESFLRYLTLPQDNELAIDLRQTAVVIMAHLDRLATPCMPPQCSSPTSHKGSLQEVIGWGLIGWKYYANVIGPIQCEGLANLGITQIACAEKRFLILSRNGRVYMQAYNSDTLGPQLVQGLASRNIVKIAAHSDGHHYLALAATGEVFSWGCGDGGRLGHGDTVPLEEPKMISALSGKQAGKHVVHIACGSTYSAAITAEGELYTWGRGNYGRLGHGSSEDQTIPMLVTGLKGLKVIDVSCGSGDAQTLAVTENGQVWSWGDGDYGKLGRGGSDGCKTPKLIEKLQDLDVVKVRCGSQFSVALTKDGQVYSWGKGDNQRLGHGTEEHVRYPKLLEGLQGKKVVDVAAGSTHCLALTEDSEVHSWGSNDQCQHFDTLRITKPEPTALPGLDTKHIVGIACGPAQSFAWSSCSEWSIGLRVPFVVDVCPMTFEQLDLLLRQVSEGMDGTSDWPPPQEKECMAVATLNLLRLQLHAAISHQVDPECLGLGLGSVLLNSLKQTVVTLASNAGVLNTVQSAAQAVLQSGWSVLLPTAEERARALSALLPSAVSGNDMNVSPGRRFMIDLLVGSLMADGGLESALNAAITAEIQDIEAKKEAQKEKEIDEQEANASTLHRSRTPLDKDLINTGIYESAGKQSLPLVQLIQQLLRNIASQTIAKLKDVARRILSCIEHDQYSQDRSASLDLLLRFQRLLVSKLYPGDSGGQVSNSFSPELLGVGSLLKKYTALLCTHIGDILPVATSIASSSYRHFAEVSRVVDGDLTGVLLPELIVSIVLLLNKSPGLMQEAGAIPLLAGLLEHLDRFNHLAPGKERDDNEDLAWPGIMGSFFTGQNSRNNEEVTLIRKADLENHNKDGGFWTVIDGKVYDIKDFQTQSLTGNSILAQFAGDDPVVALEAALQFEDTRDSMHAFCVGQYMEPDQEVITTPDLTSLSSPLIDPERNLGLLLGLHASYLAMSTPLSPVEIECAKWLKSSIFSGGLQTSQIHYSYNEEKDEDHCSSPGNTTPNKSKLYSHRLTLSDHSQPFLQAIADNNIQDHTVKDFLCQIERYCKQCHLTTPITFPPEHPVEEVGRLLLCCLLKHEDLGHVALSLVHPVTLGVDQVKHRTLPKSVVDVCRVVYQAKCSLIKTHQEQGRSYKEVCAPVIERLRFLFNELRPAVCNDLSIMSKLKLLSSLPRWRRIAQKMIRERRKLKRVPKKSEPTDVEESKIGNEEGDLEEACILPHSPVSVDKRPVPVKSPKDKWQPLLSTVTGVHKYKWLKQNVQGLYPQSALLSTIVEFALKEEPVDVEKMRKCLLKQLERAEVRLEGIDTILKLANKNFLLSSVQYAMFCGWQRLIPEGTNIGEPLTDCLKDVDLIPPFNRMLLEVTFGKLYGWAIQNIRNVLLDANARFKELGIQPVPLQTITNENPAGPSLGTIPQARFLLVMLNMLTLQYGANNLNLLLNSGMLALTQTTLRLIGPSSDNIEEEMNASARGASATVLEESRKETTPVQLPVSGPELAAMMKIGTRVMRGVDWKWGDQDGPPPGLGRVIGELGEDGWIRVQWDTGSTNSYRMGKEGKYDLKLAEPPPAAQPSTEDSDTEDDSESEQMERNFHPTSIMLTSTVNLLQTLCLCAGIHAEIMQNEATRTLCGLLRMLVESGTVDKTSSLPNKMVYKEQHRSWCTLGFIRSIALMPQMCSTLSTSQWITLLMKIVEGHESFTAASLQRQILAVHLLQAVLPSWDKTERSRDMKFLVEKLFGFLGSLLSTCSSDIPLLRESTLRRRKSRPQASLTATHSSTLAEEIVALLRTLHSLSQWNGLINKYINSQLNSITHIFAGKQSEGTVLEDYFPDSENPEVDGLMAVLAVIGGIDSRLRLGGQVVHDEFGEGTVTRITPKGKITVQFCDMRICRICPLSQLKPLPVMAFNVNNLPFTESMLSVWAQLVNLAGSKLEKHRIKKSTNQALTGQVDLDLLRCQQLKLYILKAGRALLSHQDKLRQILSQPALQENGVLSTDDGTASPDIGDMSPEGPQPPMILLQQLLTSATQPSPVKAIFDKQELEAAALAVCQCLAVESTHPSTPVFEECSSSEATTPVTVQHIRSVKVKKRKQSPLPALPIVVQLMEMGFPRKNIEFALKSLTGSASGLPGVEALVGWLLDHPDVQITDLSDADTVSDEYSDDEIVEDVEETEASYPVSSCAVVTESHTYKKRADFLSNDDYAVYVRENIQVGMMVRCCRTYEEVCEGDVGKVIKLDRDGLHDLNVQCDWQQKGSTYWVRYIHVELIGFPPQSSPSHIKIGDKVRVKTSVTTPKYKWGSVTHRSVGVVKAFSANGKDVIVDFPQQSHWTGLLSEMELVPSIHPGVTCDGCQMFPINGPRFKCRSCDDFDFCETCFKTKKHNTRHSFGRINEPGQSLVFCGRSGKQLKRCHSSQPGMLLDNWSRIVKNLNVSSSVNQASRLIDGSEPCWQSSGSQGKHWIRLEIFPDVLVHRLKMIVDPADSSYMPSLVVVSGGNSLNNLIELKTININPADTTVPLLNDCTEYHRYIEVAIKQCRSSGIDCKIHGLIILGRIRAEDEDLAAVPFLASDNEEEDDEKANTGSLIRKKASGLESAATIRTKVFVWGLNDKDQLGGLKGSKIKVPSFSETLSALNVVQVAGGSKSLFAVTVEGKVYACGEATNGRLGLGISSGTVPIPRQITALSNYVVKKVAVHSGGRHAMALTVDGKIFSWGEGDDGKLGHFSRMNCDKPRLIEALKTKRIRDIACGSSHSAAITSSGELYTWGLGEYGRLGHGDNTTQLKPKMVKVLLGHRVIQVACGSRDAQTLALTDEGLVFSWGDGDFGKLGRGGSEGCNIPQNIERLNGQGVCQIECGAQFSLALTKSGVVWTWGKGDYFRLGHGTDVHVRKPQVVEGLRGKKIVHVAVGALHCLAVTDTGQVYAWGDNDHGQQGNGTTTVNRKPTLVQGLEGQKITRVACGSSHSVAWTTVDVATPSVHEPVLFQTARDPLGASYLGVPSDADSSAASNKISGVNSSKPNRPSLAKILLSLDGNLAKQQALSHILTALQIMYARDAVVGALMPASMIAPVECSSSSSPAPPSDVSSAGSPMNGEESVLAVDVEDRLSPNPWQDKRGEVVSSEDAVTPSAVTPSASSASSRPFIPVTDDPGAASIIAETMTKTKEDVESQNKVSGPEPQYLDEFTSLLVPDDTRVMVDLLKLSVSNRAGEKGKDVLSAVLSGMGTAYPQVADMLLELCVTELEDVATDSQSGRLSSQPVVVESSHPYTDDTSTSGTVKIPGAEGLRVEFDRQCSTERRHDPLTIMDGVNRIVSVRSGREWSDWSSELRIPGDELKWKFISDGSVNGWGWRFTVYPIMPAAGPKDLLSDRCILSCPSMDLVTCLLDFRLNLASNRSIVPRLAASLAACAQLSALAAGHRMWALQRLRKLLTTEFGQSININRILGDNDGETRTMSFTGSALAALVKGLPEALQRQFEYEDPIVRGGKQLLHSPFFKVLVALACDLELDTLPCCAETHKWAWFRRYCMASRVSVALDKRTPLPRLFLDEVAKKIRELMADSENLDVLHESHDIFKREQDEQLVQWMNSDVSIVGDLMTGLFLLEAVEQFMVGVTIIEDSLAVLREQKSKFQHLVKLLQLCGPYS

Target ORF information:

RefSeq Version	XM_031959091.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii HECT and RLD domain containing E3 ubiquitin protein ligase 2 (HERC2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031959091.1

ORF Insert Sequence:

ATGTCTTCTG AATCCTTCTG TTTAGCTGCC CAGGCTAGAT TTGACTCCAA GTGGTTAAAA 
ACAGATTTAC AGCTTGCTTT TACAAGAGAT GGACTCTGTG GCCTATGGAA TGAAATGGTC 
AAAGATGGAG AAATTGTATA TCCTGGGACA GATGTAATAC AGAATGGAGA ACTACCTCCT 
CGAAAAGATG ACACCATGGA ATGTCAGAGT GGAACAAAGA AAGAAGATCA AAATGATAAA 
GAGAAGAAAG ATGAAGAGGA AATTCCAGTC CCTATGTATA AGGCCAAATC ACTTGTGGAG 
AGTTGGGTAT GGGGCAAACA ACCAGATGTG AACGAGTTGA AAGAGTGCCT TTATGTGCTA 
GTGAAAGAAC AGCAAACTCT TGCTACACGG ATGGCTACAA CAACTCTTTC AGCAGTAAGG 
CTAAAGCAGA GGCTGGTTAT ACTGGAACGA TATTTCATTG CACTGAATAG AACTGTTCTT 
CAGGAAAATG TCAAAGTTAA GTGGAAAAGC AACAGTATTC CTTTACCTAC TGTCGACAAA 
AAAAGTTCTA GACCTATAGG AAAAGGTGTA GAAGGACTTG CAAGAGTTGG ATCCAGAGCA 
GCCCTTTCAT TTGCTTTTGC ATTCCTACGC AGAGCTTGGC GGTCAGGGGA AGATGCCGAT 
CTATGCAGTG AATTGTTACA GGAGTCTCTT GATGCCTTGA GAGCCCTACC AGAAGCATCT 
CTTTTTGATG AAAGCACAGT GTCATCTGTT TGGTTGGAAG TTGTGGAGAG AGCAACTAAA 
TTCCTAAGGT CTGTTGTTAC TGGTGATGTT CATGGAACTG CAAGTACCAA AGGACCAGGA 
AACATTCCAT TACAAGACCA GCACTTAGCA CTTGCCATAT TGTTAGAGTT GGCTGTGCAG 
AGAGGCACTC TAAGCCAAAT GTTATCTGCC ATTATGTTAC TACTTCAATT ATGGGACAAT 
GGGACAAGGG AAACAGATAA TGAACGATCT GCCCAAGGAA CTAGTGCACC ACTTCTCCCT 
TTATTACAAA GATTTCAGAG TATAATCTGC AATAAAGACA TACTTAATAC TGAAGATGAG 
ATTCAGATGC TGTCTGGTCT TCTGAGTCCC AATGAAAGTT TTTTGAGGTA CCTTACTTTA 
CCACAAGACA ATGAATTAGC CATTGATTTG CGTCAAACTG CAGTAGTGAT TATGGCGCAT 
TTAGATCGTC TGGCCACTCC CTGTATGCCT CCTCAGTGTA GTTCTCCAAC ATCTCATAAG 
GGATCTTTAC AAGAAGTCAT AGGTTGGGGC TTAATAGGAT GGAAATACTA TGCTAATGTG 
ATTGGCCCAA TTCAATGTGA AGGACTTGCA AATCTTGGGA TCACACAGAT TGCCTGTGCA 
GAAAAGCGTT TCCTGATTTT GTCTCGGAAT GGGCGAGTTT ACATGCAGGC TTACAACAGT 
GACACATTGG GACCCCAACT AGTTCAAGGT CTTGCCTCTA GAAATATTGT GAAAATTGCA 
GCACATTCAG ATGGTCATCA TTATCTGGCT TTGGCAGCCA CTGGAGAGGT CTTTTCTTGG 
GGCTGTGGAG ATGGTGGACG GTTAGGCCAT GGTGATACAG TGCCTCTAGA AGAACCTAAG 
ATGATATCTG CTCTCTCAGG AAAGCAAGCT GGGAAACATG TAGTTCATAT CGCATGTGGA 
AGTACTTATA GTGCAGCTAT TACAGCTGAA GGAGAGTTAT ATACTTGGGG TCGAGGAAAC 
TATGGCAGGC TGGGACATGG CTCAAGTGAA GATCAGACAA TTCCAATGCT AGTAACTGGA 
CTTAAAGGAC TGAAGGTTAT TGATGTCTCT TGTGGGAGTG GGGATGCTCA GACTCTTGCA 
GTGACTGAGA ATGGTCAAGT CTGGTCTTGG GGCGATGGTG ATTATGGAAA ACTAGGAAGA 
GGTGGTAGTG ATGGTTGTAA AACACCAAAA CTGATTGAAA AACTTCAAGA CTTAGATGTT 
GTTAAAGTAC GTTGTGGAAG CCAATTTTCC GTTGCTTTAA CAAAAGATGG TCAAGTTTAC 
TCGTGGGGAA AAGGTGACAA TCAGAGACTT GGGCATGGAA CAGAGGAGCA TGTTCGATAT 
CCGAAACTGT TAGAAGGCTT ACAAGGTAAA AAAGTCGTTG ATGTGGCTGC TGGTTCTACA 
CACTGCTTAG CACTTACTGA GGATAGCGAA GTTCATAGCT GGGGGAGTAA TGATCAGTGT 
CAGCACTTTG ACACCCTTCG GATTACCAAA CCTGAACCTA CTGCATTGCC AGGATTAGAT 
ACAAAACATA TAGTTGGAAT TGCTTGTGGG CCCGCTCAGA GTTTTGCTTG GTCTTCTTGT 
TCTGAGTGGT CCATTGGCCT ACGTGTACCT TTTGTGGTGG ATGTTTGTCC CATGACATTT 
GAACAGTTGG ATCTCTTACT ACGACAAGTA AGTGAGGGAA TGGATGGCAC CTCTGACTGG 
CCACCACCAC AGGAGAAAGA ATGTATGGCA GTGGCAACAT TGAACCTTCT GAGACTTCAG 
TTGCATGCTG CTATTAGTCA CCAGGTTGAT CCGGAATGTC TTGGATTGGG TCTGGGAAGT 
GTACTCTTGA ATAGCTTGAA GCAGACAGTT GTAACACTAG CAAGTAATGC TGGAGTTCTT 
AATACAGTGC AATCAGCTGC TCAAGCAGTA CTGCAGAGTG GATGGTCTGT GCTGCTTCCA 
ACTGCAGAGG AACGAGCAAG GGCTCTTTCA GCTCTCTTAC CCAGTGCAGT TTCAGGAAAT 
GACATGAATG TAAGTCCAGG ACGTCGTTTT ATGATTGATC TTCTTGTAGG CAGTTTGATG 
GCTGATGGAG GATTAGAATC AGCATTAAAT GCTGCCATCA CTGCAGAAAT CCAGGATATT 
GAAGCAAAAA AAGAAGCTCA AAAGGAAAAA GAGATTGATG AGCAGGAAGC AAATGCTTCA 
ACACTTCATC GGAGTAGGAC ACCATTAGAT AAAGACCTCA TTAATACTGG AATTTATGAA 
TCTGCCGGAA AACAGAGTTT GCCTCTGGTT CAATTAATAC AACAGCTTCT TAGAAATATT 
GCTTCCCAGA CAATAGCCAA ATTGAAAGAT GTTGCTCGTC GAATTTTGTC CTGTATAGAA 
CATGATCAAT ATAGTCAAGA CAGATCTGCC TCCCTGGATT TGTTGCTTCG TTTTCAGCGT 
CTGCTGGTCA GTAAACTCTA TCCAGGAGAC AGTGGTGGTC AAGTTTCAAA TTCCTTTAGT 
CCTGAGCTAT TAGGAGTTGG TTCTTTGCTG AAAAAATATA CAGCTCTTTT GTGCACACAC 
ATTGGAGATA TATTGCCTGT GGCAACTAGT ATTGCATCTT CCAGTTATCG GCATTTTGCA 
GAAGTATCTC GTGTTGTGGA TGGTGATCTA ACAGGTGTTC TTCTTCCAGA GTTGATAGTA 
TCTATTGTCC TTTTGCTCAA TAAAAGTCCT GGATTAATGC AGGAAGCTGG GGCAATTCCT 
CTCTTAGCTG GTCTACTGGA ACATCTAGAT AGATTTAACC ATTTGGCACC AGGAAAAGAG 
AGAGATGATA ATGAGGATTT AGCCTGGCCT GGAATAATGG GATCATTTTT TACAGGTCAG 
AATAGTAGAA ATAATGAGGA AGTGACGCTC ATACGTAAAG CTGATCTGGA GAATCACAAT 
AAAGATGGAG GCTTCTGGAC TGTGATTGAC GGGAAAGTGT ATGATATAAA AGACTTTCAG 
ACACAGTCTT TAACAGGCAA TAGCATTCTT GCTCAGTTTG CAGGTGATGA CCCGGTGGTT 
GCTTTGGAAG CTGCTTTACA GTTTGAGGAC ACAAGGGATT CAATGCATGC ATTTTGTGTT 
GGCCAGTATA TGGAGCCTGA CCAGGAAGTG ATTACAACGC CAGACCTTAC CAGTCTCTCT 
TCACCTCTCA TAGATCCAGA AAGAAATCTG GGTCTCCTCC TTGGACTTCA TGCTTCCTAT 
TTAGCAATGA GCACACCACT ATCTCCTGTG GAGATTGAAT GTGCCAAATG GCTTAAGTCA 
TCCATATTCT CTGGAGGATT GCAAACCAGT CAGATACATT ATAGTTACAA TGAAGAGAAA 
GATGAAGACC ACTGCAGTTC TCCTGGAAAT ACTACTCCAA ACAAATCCAA ACTCTATTCC 
CATAGATTGA CTTTGAGTGA TCATTCCCAA CCATTTTTGC AAGCCATTGC AGATAATAAC 
ATTCAGGATC ATACCGTGAA GGATTTTTTA TGTCAGATAG AAAGGTATTG CAAGCAATGC 
CATTTGACCA CACCGATAAC ATTTCCTCCT GAACATCCTG TGGAAGAAGT GGGACGTTTA 
CTCTTATGTT GTCTTCTAAA GCATGAAGAT TTAGGTCATG TGGCCTTATC TTTAGTTCAC 
CCAGTTACAC TTGGTGTTGA CCAAGTCAAA CACAGAACGT TGCCTAAGTC TGTAGTGGAT 
GTTTGTAGAG TCGTCTACCA GGCAAAATGC TCACTAATAA AGACACATCA GGAACAGGGA 
CGTTCTTATA AGGAAGTGTG TGCTCCTGTT ATTGAACGTT TGCGATTTCT TTTTAATGAA 
TTGCGTCCTG CAGTCTGCAA TGACCTCTCA ATAATGTCCA AACTTAAACT TTTAAGCTCT 
TTGCCTCGTT GGAGAAGAAT AGCTCAGAAG ATGATACGAG AGAGAAGGAA ATTAAAAAGA 
GTACCCAAAA AGTCAGAACC TACTGATGTG GAAGAATCTA AGATTGGCAA TGAAGAGGGT 
GACTTAGAAG AAGCCTGTAT TCTTCCTCAT AGTCCTGTAT CTGTAGATAA AAGACCTGTT 
CCAGTTAAAT CACCTAAGGA TAAATGGCAG CCACTTCTGA GTACAGTTAC AGGTGTTCAC 
AAATACAAAT GGCTTAAACA GAATGTTCAA GGCCTTTATC CTCAGTCTGC ACTGCTTAGC 
ACAATTGTTG AATTTGCGCT TAAAGAAGAG CCAGTTGATG TGGAAAAGAT GAGAAAGTGC 
TTACTCAAAC AGTTGGAGAG AGCAGAAGTT CGTTTGGAAG GAATAGACAC GATTTTAAAA 
TTGGCAAATA AAAATTTCTT GCTTTCGTCT GTGCAATATG CTATGTTCTG TGGATGGCAA 
AGACTTATCC CAGAAGGAAC CAATATAGGG GAACCACTTA CAGATTGTTT AAAGGATGTT 
GATCTGATTC CACCATTTAA TCGAATGCTA CTAGAAGTAA CTTTTGGAAA GTTGTATGGT 
TGGGCCATTC AGAATATTCG AAATGTTTTG TTGGATGCAA ATGCCAGATT TAAAGAGCTA 
GGTATCCAGC CTGTTCCTCT GCAAACTATT ACTAATGAGA ATCCAGCAGG GCCAAGCCTT 
GGAACCATCC CACAAGCACG TTTTCTCCTG GTTATGCTCA ACATGCTTAC CCTTCAATAT 
GGTGCAAACA ACTTGAATCT TCTTTTGAAC TCTGGCATGC TAGCATTGAC TCAGACAACA 
TTGCGATTAA TAGGTCCTAG TTCTGATAAT ATCGAGGAAG AAATGAATGC CTCTGCACGA 
GGAGCTTCAG CTACTGTTTT GGAAGAATCC AGAAAAGAAA CCACTCCAGT ACAACTTCCT 
GTTTCAGGGC CAGAACTGGC AGCAATGATG AAAATTGGTA CCAGGGTCAT GAGGGGAGTG 
GACTGGAAAT GGGGAGATCA GGATGGTCCA CCTCCAGGTT TAGGTCGTGT GATTGGTGAA 
TTGGGAGAGG ATGGATGGAT AAGGGTTCAG TGGGATACAG GCAGTACTAA TTCATACCGA 
ATGGGAAAGG AAGGAAAGTA TGATCTTAAA TTGGCTGAGC CTCCACCTGC AGCTCAGCCC 
TCAACAGAGG ATTCGGACAC TGAGGATGAT TCTGAAAGTG AACAAATGGA AAGAAACTTC 
CACCCTACTT CTATTATGCT TACAAGTACT GTTAATTTAC TCCAAACTCT CTGTCTCTGT 
GCTGGAATTC ATGCAGAAAT CATGCAAAAT GAAGCTACAA GAACTCTGTG TGGTCTACTT 
AGAATGTTAG TGGAAAGTGG AACAGTAGAT AAAACATCTT CTTTACCAAA TAAGATGGTT 
TATAAAGAGC AGCACAGAAG TTGGTGTACA CTTGGATTTA TTCGAAGTAT AGCACTTATG 
CCTCAAATGT GTAGCACCCT TAGCACATCT CAGTGGATCA CATTACTAAT GAAAATTGTG 
GAAGGACACG AGTCATTTAC AGCAGCTTCG CTTCAGAGAC AGATCTTAGC AGTACATTTA 
CTACAAGCTG TACTTCCATC TTGGGACAAA ACAGAAAGAT CAAGAGATAT GAAGTTTCTT 
GTGGAAAAAC TTTTTGGCTT CTTGGGCAGT CTACTTAGTA CATGCTCTTC AGACATACCT 
TTGCTCAGAG AATCAACTCT GAGAAGGAGG AAGTCCAGAC CCCAGGCATC TTTGACTGCA 
ACCCATAGTA GTACTTTGGC AGAGGAGATA GTGGCCCTGC TAAGGACTCT ACATTCACTG 
AGCCAGTGGA ATGGACTCAT AAACAAGTAC ATCAACTCTC AGCTAAACTC CATCACCCAC 
ATCTTTGCAG GAAAACAGTC TGAAGGGACT GTGTTAGAAG ATTACTTTCC TGATTCTGAG 
AACCCTGAAG TTGATGGCCT AATGGCAGTG CTAGCTGTAA TTGGTGGAAT AGATAGTCGA 
TTGAGGCTGG GTGGCCAAGT GGTACATGAT GAATTTGGAG AAGGCACAGT GACACGCATC 
ACTCCTAAAG GCAAAATCAC AGTCCAGTTC TGTGATATGC GAATATGTAG AATTTGTCCA 
TTGAGTCAGC TAAAACCGCT TCCAGTTATG GCATTTAATG TGAACAACTT ACCATTCACG 
GAATCTATGC TCTCTGTCTG GGCTCAATTG GTCAACTTGG CAGGGAGCAA ATTAGAAAAA 
CACAGAATTA AGAAATCTAC TAATCAAGCC CTCACAGGAC AAGTAGACTT GGATTTGCTA 
AGATGCCAAC AGTTGAAATT ATACATATTG AAAGCAGGTC GAGCATTGCT TTCCCATCAG 
GATAAACTGA GGCAGATCTT ATCTCAACCA GCTTTACAAG AGAATGGAGT TCTCTCTACA 
GATGATGGTA CAGCATCCCC TGATATTGGA GATATGTCTC CTGAAGGACC TCAACCTCCA 
ATGATCCTTT TACAGCAATT GTTGACATCT GCCACACAGC CATCACCTGT AAAAGCAATA 
TTTGACAAAC AGGAACTTGA GGCTGCTGCT TTGGCTGTAT GCCAGTGTCT AGCTGTGGAG 
TCAACTCATC CATCAACTCC AGTGTTTGAA GAATGTAGCT CTAGTGAGGC CACAACACCT 
GTCACAGTCC AGCATATTCG ATCTGTAAAA GTGAAAAAGC GAAAGCAGTC TCCTCTCCCT 
GCTTTGCCTA TTGTAGTACA GCTCATGGAA ATGGGATTTC CGAGGAAAAA CATTGAATTT 
GCATTGAAGT CTCTTACTGG GAGTGCTTCA GGGTTACCAG GTGTTGAAGC CTTGGTTGGT 
TGGTTGCTGG ATCATCCTGA TGTCCAAATT ACTGATCTCT CAGATGCTGA TACAGTCTCT 
GATGAATACT CTGATGATGA AATAGTGGAA GATGTGGAAG AGACTGAAGC ATCTTACCCT 
GTGTCTAGTT GTGCTGTTGT AACGGAGAGT CACACTTATA AAAAACGAGC TGATTTCTTA 
AGTAATGATG ACTATGCAGT ATATGTTAGA GAAAACATTC AGGTAGGAAT GATGGTTAGG 
TGCTGCAGAA CGTATGAAGA AGTTTGTGAA GGAGATGTAG GTAAAGTGAT TAAATTGGAC 
AGAGATGGAT TACACGACCT CAATGTGCAG TGTGATTGGC AACAAAAGGG GAGTACCTAT 
TGGGTTAGGT ATATCCATGT TGAACTTATA GGTTTCCCAC CACAGAGTTC TCCTTCCCAT 
ATCAAGATTG GTGATAAAGT TAGGGTGAAA ACATCAGTAA CCACACCAAA ATATAAATGG 
GGATCAGTTA CTCATAGAAG TGTGGGAGTT GTGAAAGCTT TTAGTGCCAA TGGAAAAGAT 
GTTATTGTGG ATTTTCCTCA GCAGTCTCAT TGGACAGGAT TATTGTCAGA AATGGAATTG 
GTGCCTAGTA TTCATCCTGG AGTTACGTGT GATGGCTGCC AAATGTTTCC TATCAATGGT 
CCTCGTTTCA AATGCAGGAG TTGTGATGAT TTTGACTTTT GTGAGACCTG TTTTAAAACC 
AAGAAGCATA ATACCAGACA TAGTTTTGGC AGAATAAATG AACCAGGTCA GTCTCTTGTA 
TTTTGTGGGC GTTCTGGGAA ACAGCTAAAA CGTTGCCACA GTAGTCAGCC TGGAATGCTC 
TTGGATAATT GGTCAAGAAT TGTTAAGAAT TTAAATGTGT CCTCATCAGT AAATCAGGCT 
TCTCGCTTGA TTGATGGCAG TGAACCATGT TGGCAGTCAT CTGGATCACA AGGAAAGCAT 
TGGATTCGAC TGGAGATTTT CCCCGATGTT CTTGTTCATA GACTGAAAAT GATAGTAGAT 
CCTGCTGACA GTAGCTACAT GCCTTCACTA GTTGTAGTTT CAGGTGGGAA TTCCCTAAAT 
AATTTAATTG AACTGAAGAC AATCAATATT AACCCAGCTG ATACCACAGT GCCTCTTCTA 
AATGACTGCA CAGAGTATCA CAGGTATATT GAGGTTGCCA TAAAGCAATG CAGAAGCTCA 
GGAATAGATT GTAAAATTCA TGGTCTTATT ATACTGGGAC GAATTCGGGC AGAAGATGAA 
GATTTGGCTG CTGTTCCTTT CTTAGCGTCA GATAATGAAG AAGAAGATGA TGAAAAAGCT 
AACACTGGGA GTCTTATTAG GAAGAAGGCT TCTGGATTGG AATCAGCAGC CACAATAAGG 
ACCAAAGTTT TTGTCTGGGG GCTGAATGAC AAGGATCAAT TAGGAGGATT AAAGGGTTCT 
AAGATAAAAG TTCCTTCATT CTCTGAAACC CTTTCTGCTT TGAATGTAGT TCAAGTGGCA 
GGTGGATCTA AAAGTTTGTT TGCAGTGACT GTGGAGGGTA AAGTGTATGC TTGTGGAGAA 
GCTACTAATG GACGACTGGG TTTAGGTATT TCTAGTGGAA CTGTACCCAT TCCGCGGCAG 
ATTACAGCTC TCAGCAATTA TGTGGTAAAG AAAGTTGCTG TTCACTCAGG TGGCCGGCAT 
GCTATGGCTT TAACTGTTGA TGGAAAGATA TTTTCATGGG GTGAAGGTGA TGATGGAAAA 
CTAGGACACT TCAGCAGAAT GAACTGTGAT AAACCAAGAC TGATAGAGGC ATTGAAAACC 
AAACGTATTC GTGATATAGC TTGTGGTAGT TCTCACAGTG CTGCCATTAC ATCAAGTGGT 
GAATTGTATA CATGGGGCCT TGGAGAATAT GGCCGGTTGG GACATGGGGA TAATACTACA 
CAGCTGAAAC CCAAAATGGT GAAAGTTCTT CTTGGTCATC GAGTTATTCA AGTTGCTTGT 
GGAAGTAGAG ATGCCCAGAC TCTGGCTTTG ACAGATGAAG GTTTGGTCTT TTCCTGGGGA 
GATGGTGACT TTGGGAAACT GGGCCGGGGA GGAAGTGAAG GATGTAACAT TCCTCAGAAC 
ATTGAGAGAC TTAATGGACA AGGAGTTTGC CAGATTGAGT GTGGGGCTCA ATTTTCCTTG 
GCACTTACAA AGTCAGGAGT AGTATGGACT TGGGGTAAAG GAGATTACTT TAGGCTTGGT 
CATGGCACTG ATGTTCATGT GAGAAAACCA CAAGTAGTAG AAGGACTACG AGGAAAGAAG 
ATAGTTCATG TGGCAGTAGG CGCTCTTCAC TGCTTAGCTG TCACTGATAC AGGTCAAGTG 
TATGCTTGGG GTGACAATGA TCATGGACAA CAAGGCAATG GGACTACTAC TGTCAACAGA 
AAGCCTACCC TTGTACAAGG CTTAGAAGGA CAAAAAATCA CTCGGGTAGC TTGTGGCTCA 
TCTCATAGTG TGGCGTGGAC AACAGTGGAT GTAGCAACCC CATCTGTTCA TGAACCTGTC 
CTCTTTCAAA CTGCTAGGGA CCCTTTAGGC GCTTCTTACT TAGGTGTTCC TTCCGATGCT 
GATTCTTCTG CTGCCAGTAA CAAAATCAGT GGTGTAAATA GTTCCAAGCC TAACCGCCCT 
TCCCTTGCTA AGATTCTCTT GTCTTTGGAT GGAAACCTTG CCAAACAGCA AGCATTATCT 
CATATACTTA CAGCATTGCA AATTATGTAT GCGAGAGATG CAGTAGTTGG AGCATTAATG 
CCTGCTAGTA TGATCGCACC AGTGGAATGC TCATCTTCTT CATCACCTGC ACCCCCCTCA 
GATGTGTCTT CAGCAGGAAG TCCCATGAAT GGTGAAGAGT CTGTGTTAGC TGTGGATGTA 
GAAGATAGAC TAAGCCCAAA TCCATGGCAA GACAAGAGAG GGGAGGTAGT TTCTTCAGAA 
GATGCTGTGA CTCCATCAGC AGTGACTCCC TCAGCTTCCT CTGCCTCTTC TCGACCATTT 
ATTCCAGTAA CTGATGATCC TGGAGCTGCC AGTATCATTG CAGAAACCAT GACTAAAACC 
AAAGAAGATG TTGAAAGCCA AAATAAAGTA TCAGGTCCGG AACCTCAATA TTTGGATGAA 
TTTACTAGTC TCTTAGTGCC AGATGATACC CGTGTCATGG TTGACTTGCT CAAGCTGTCT 
GTTTCTAATC GAGCTGGTGA AAAGGGAAAA GATGTTCTTT CAGCTGTGCT TTCTGGTATG 
GGCACTGCTT ATCCACAGGT AGCTGATATG TTGTTGGAGC TCTGTGTTAC AGAGTTAGAA 
GATGTTGCCA CAGACTCTCA GAGTGGTCGA CTCTCTTCCC AACCTGTTGT AGTAGAAAGT 
AGTCATCCGT ATACTGATGA TACCTCTACC AGTGGTACTG TCAAAATACC AGGTGCTGAA 
GGACTCAGAG TAGAGTTTGA TCGACAATGT TCTACAGAAC GGCGTCATGA CCCTCTCACA 
ATCATGGATG GTGTCAATAG GATAGTTTCA GTTCGATCAG GTCGAGAATG GTCTGATTGG 
TCCAGTGAAC TTCGAATTCC TGGAGATGAA TTAAAGTGGA AGTTCATCAG TGATGGATCA 
GTGAATGGAT GGGGTTGGCG ATTCACTGTC TATCCTATTA TGCCAGCTGC AGGCCCTAAA 
GATCTTCTTT CAGATCGGTG TATTCTTTCC TGTCCCTCAA TGGACTTGGT TACATGCTTG 
TTGGACTTTA GATTAAATTT AGCTTCCAAT AGAAGTATTG TTCCTCGTTT GGCAGCATCA 
CTTGCAGCAT GTGCTCAATT GAGTGCTCTA GCTGCTGGTC ACAGAATGTG GGCCCTTCAA 
AGACTGAGGA AACTGCTTAC AACTGAATTT GGACAATCAA TCAATATAAA TAGGATACTT 
GGAGATAATG ATGGAGAAAC AAGAACCATG AGTTTTACCG GTAGTGCTTT AGCAGCTTTG 
GTAAAAGGTC TTCCAGAAGC TTTGCAAAGA CAGTTTGAAT ATGAAGATCC GATTGTTAGA 
GGAGGCAAAC AACTGCTCCA TAGCCCTTTC TTTAAGGTGC TGGTAGCTCT TGCTTGTGAC 
CTAGAACTGG ATACTCTTCC TTGCTGTGCT GAGACACACA AATGGGCTTG GTTTAGAAGA 
TACTGTATGG CCTCTAGAGT TTCAGTAGCC TTGGACAAAC GAACACCATT ACCTCGCCTT 
TTCCTTGATG AGGTGGCAAA GAAAATCAGG GAGTTAATGG CCGACAGTGA AAATCTGGAT 
GTTCTGCATG AGAGCCATGA CATTTTTAAG AGAGAACAAG ATGAACAACT CGTCCAGTGG 
ATGAACAGTG ATGTATCCAT TGTAGGAGAC CTGATGACTG GACTCTTTCT GCTGGAGGCA 
GTGGAACAAT TTATGGTTGG GGTCACAATC ATCGAGGACA GCTTGGCGGT ATTGAGGGAG 
CAAAAGTCAA AGTTCCAACA CCTTGTGAAG CTCTTGCAGC TCTGCGGCCC ATACAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HERC2 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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