LOC106599291 cDNA ORF clone, Salmo salar(Atlantic salmon)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC106599291
Entrez Gene ID	106599291
Full Name	E3 ubiquitin-protein ligase HECW1-like
Gene Type	protein-coding
Organism	Salmo salar(Atlantic salmon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014190451.1	XP_014045926.1	E3 ubiquitin-protein ligase HECW1-like isoform X2
XM_014190454.1	XP_014045929.1	E3 ubiquitin-protein ligase HECW1-like isoform X3
XM_014190450.1	XP_014045925.1	E3 ubiquitin-protein ligase HECW1-like isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC106599291 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC106599291 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAt11994	XM_014190450.1 Latest version!	Salmo salar E3 ubiquitin-protein ligase HECW1-like (LOC106599291), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAt11995	XM_014190451.1 Latest version!	Salmo salar E3 ubiquitin-protein ligase HECW1-like (LOC106599291), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAt11996	XM_014190454.1 Latest version!	Salmo salar E3 ubiquitin-protein ligase HECW1-like (LOC106599291), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC106599291 ( XM_014190450.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_014045925.1 Salmo salar E3 ubiquitin-protein ligase HECW1-like (LOC106599291), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAt11994
Clone ID Related Accession (Same CDS sequence)	XM_014190450.1
Accession Version	XM_014190450.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	E3 ubiquitin-protein ligase HECW1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012333553.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCAAA GGCAGGTGAC ACTGACCCAG AACTTGTACC AGAACAGGTT TCTCGGACTC 
AGACTTGCAG CCATGGCCTC CCCATGTCGT TCAACCCAGA ACCGTCGGCG CTGTAAGGAC 
CCCATCCGCC ACAGCTACAA CCCCGAACAA TTTCGCAACG TTAATTTCCA GAATGGGGTG 
CCGGAGGACG TGGCAGCCAT GTACCGCTCC ACCAGCGACA CGGACCTGGT GACATCACAC 
AGCCGCTCCA CGCTTACCGT CAGCACCTCG CTCTACGCCA TCGGACAGAC CCAGGACATC 
CTCATCTGCT GGGACATCAA GGAGGAGGTG GACGCTGGGG ACTGGATCGG CATGTACCTC 
ATTGAAGAGG TGTCGTCGGA GAACTTTCTG GACTACAAGA GCCGCGGAGT GAATGGTTCC 
CACAAGGGCC AGATAGTGTG GAAGATTGAG GGCAACTCCT ATTTTGTGGA GCCGGAGACT 
AAGATCTGTT TTAAATATTA CCATGGTGTG AGCGGGGCCC TGCGAGCCAC TACCCCCAGT 
TTGACTGTGA AGAACCCCTC AGTTCCGGTA TTTAAGCCCG TCGCCAATGA AGAAGTCCCC 
CAGAGCACAG GCAGTAGAAG ACTCATCAGC TTCTCCCTCT CAGATTTCCA TGCAACAGGG 
CTGAAGAAGG GGATGTTCTT CAACCCTGAC CCCTACCTTA AGATCGCCAT CCACCCCGGC 
AGGAACAGCA TCTTCCCGGC ACTGCCACAC CACGGCCAGG AGAAACGCTC AGGCATCGTC 
TGTAACACCA TCAACCCCCA CTGGCAGAGA GAGCGCTTCA ACTTTGTGTC TCTGCCCACG 
GACGTTCTGG AGATCGAGGT AAAAGACAAG TTTGCCAAGA GTCGTCCTAT CATCAAACGT 
TTCCTGGGCA AGCAGTCCAT GCCGGTGCAG AGGCTACTGG AGAAGCATGC TATTGGTGAC 
CGTGTGGTCA GCTACACGCT GGGCCGAAGG GTGCCGACGG AACATGTCAG TGGACAGCTG 
CAGTTCAGAT TTGAGATCAC TTCCTCTATT CATCCAGATG ATGAAGACAT GTCTCTCAGC 
ACAGAGCCTG AGTGTGCCGC TATGCAGGAG ACCAGTAACC AGGGGGAGGG CCAGACCTCC 
CAGGCCCAGG GCCAGGCCAA GGACACCATG AGCCTGGGTC CGGGGGCTCC CGAACTCCCC 
ATGGATGAGG AGGAAGACGC TGGAGCTCCA GAACTTCCAA TTGGAGGAGA TACAGTTACA 
GACACGCCCA TGGAGGGGCA TCCAGCAGGG CCTTTGGGTT CAGAGCAGCC CATGGATGGA 
GATGGAGATG GAGACTCAGG GTCTAGCTGT TCCAGAGCCA AGGCCCTAAA AACAGAACCA 
AGCCAACCAG AGGTCAATGG AGCTCAGGGG AAGGAGGAGG TTGCTGTGGC TGTAGCTGTG 
GCTGCAGAGA AGCTGCCCAC GGTAGAGGAT AACAGGGAGG AAGGGGGGCA GCAAACCCCC 
TCCAGTCCTC AGCTTGCTGA CCCAGAGCAG GGGGTGGACA GTACCCCAGC AGAGGGTCAG 
GTCATCCCTG CTGTATTGGA AGACATGGAG AGTGGAGCGC CTGGGGAGCC AGCAGAACAG 
GTGGTGGGTC AGGAGGAAGG GGACGAGGAA TGCTGCTCCC TGCGCATCAG GACCACCCCT 
AGGCGTAAGA ACCGGCCCTG CTCCCTGCCC GTGTCAGAGC TGGAGACAGT CATCGCCTCG 
GCCTGTGGGG AGCCCGAGAC CCCTCGCTCC CACTACATCC GCATCCACCA CCTCCTCCAC 
AGTCTGCCCT CCACCCAGCC AGGCACCCAG GAGGACGGGG AGGGAGATGG GGCTGAGGCT 
GAAACAGAGC CCGATCCAGA CCCAGGCCCT GACCAGCTCA GCCCCAGGGT CCAGGATGGC 
TCAGAAGAGG ATGAAAAGGC AGAACCCCTG ACCCCCGGGC CCAGGAGGAC CCTGCCTCGC 
AGCCTGTCCA TCGAGCGTCT GTCCGACCTG GCCCTGCTAC TGGACAGCGA GGGCCGCCAC 
CCCCAGGGCC AGGTCAGGGG TCACCAGAGA GTGTCCGACG AGGATGACAT GGAGCCCTGC 
TGCAGTGATT CCTGCTATGA GGGCCGAGGA GGCCGTCACA ACCAGGCACC TCTAGTGGCT 
GCAGCCAGGA TGTCTGTGGA GAGCAGTGAT TTCCCCTCCC AGGAGGATGA GGACATGGAG 
GTGAACGGAG CGGAGAGCAG GGAGCCAGGA GATGGGATGC CCCAGTGGCA GGTGACCCCG 
TCCACGCAGG TCCAGAGCCC CCAGAGCTTC ATAAAAAACG GAGAGTCACC CGTGGCCGGG 
CCCAGTGGGA AGATAGGACG AGATTGTCCC CTACCGTCCA GTCATCCTCC AGTCAGCCAG 
CTTCCTGCTC TGCGTCATGA TCCTCATCAT TACCCCACTA TAGATGAACC ACTTCCACCA 
AACTGGGAGG CGCGTGTGGA CAGCCACGGT CGTGTGTTCT ACGTGGACCA CATCAACAGG 
ACCACCACGT GGCAGAGACC CACCTCAGCA GCCACGCCCG ACGGCATGAG GAGGTCCGGA 
TCCGTTCAAC AGATGGAACA GCTCAATAGA AGGTACCAGA GCATCCAGAG GACCATGGCC 
ACAGACAGGA GTGAGAATGA AGGAAGCAGA AGCAGGAGTG AGAGAACAAG CCTCAACAAC 
AACAGCAGTG AGACAGACTC TCCTTCGATG GCTAATGAGC TGAGAAGAGA CAGCTCCTCT 
TCCTCCTCCT CCTCGTCATC ATCTCCAGTC AACAGTCAGA AGATCACAGC TATGTTACAG 
AGTCCAGCGG TGAAGTTCAT CACACACCCA GAGTTTTTCA CTGTCCTCCA TGCCAACTAT 
GGGGCCTACC GCATGTTCAC CAGCAGCACG TGCCTGAAGC ACATGATCCT GAAGATTCGT 
AGAGACGCTC GTAACCTTGA GAGGTACCAG CACAACAAAG ACCTGGTTAC CTTCCTCAAC 
CGCTTCGCTG ACGCCCAGCT GGAACTGCCT CGGGGCTGGG AGATCAAGAC TGACCCTCAG 
GGCAAGAACT TCTTTGTGGA CCACAACAGC CGAGCAACCA CGTTCATCGA CCCGAGAATC 
CCTCTTCAGA ACGGTTGTCT GCCCAACCAC CTGACCCACA GGCAGCACCT GCAGCGATTA 
CGCAGCTACA GTGCTGGAGA GGCCTCGGAG GTGTCCCGTA GTCGAGGTGC GGCCTTGTTG 
TCACGGCCTG GGAACAGTGT GGTGGCCGCG ATGCGGAGCC AGCATCATCC TGATCCAGTG 
CCACAAGCGT ATAATGAGAA GATAGTGGCC TTCCTCCGTC AGCCTGGAAT ACTAGAAGTT 
CTAACGGAGC GCCAGCCAGC CCTGTCCAGG AACCATTCCC TGAGGGAAAA GATCCACTAC 
ATCAGAACAG AGGGCACCGA AAGACTGGAG AAACTAGCAT GTGATGCAGA CCTGGTGATA 
CTGTTGAGCT TGTTTGAAGA GGAGATCATG TCCTATGTTC CGCCCTCCTT CCATCCTGGC 
TTCAGCTTCT CTCCGCGTTG TTCACCAGGG TCTTCACCAC AGAACTCTCC AGGAATGTCG 
AGAGCGAGAG CACCTGCCCC GTACCGGAGA GACTTTGAGG CCAAACTACG CAACTTCTAC 
AGGAAACTGG AGGCTAAAGG CTATGGCCAG GGCCCAGGAA AGATCAAGCT TATCGTCCGG 
AGAGACCACC TGCTGGAGGG TACCTTTAAC CAGGTCATGG CCTACTCACG CAAGGAACTA 
CAGAGGAATA AGCTCTACAT CACTTTCATA GGAGAGGAGG GGCTGGACTA CAGTGGGCCA 
TCCAGAGAGT TCTTCTTCCT GCTGTCTCAG GAGCTGTTCA ACCCCTACTA TGGCCTGTTT 
GAGTACTCAG CCAACGACAC ATACACCGTC CAGATCAGCC CCATGTCCGC CTTCGTAGAG 
AACCATCTGG ATTGGTTCCG GTTTAGTGGG CGTATTTTGG GACTGGTGTT GATCCACCAG 
TATCTCCTGG ATGCCTTCTT CACGCGGCCC TTCTACAAGG CTCTCCTTAG ACTAGCCACT 
GACCTGAGTG ATCTGGAATA CCTAGACGAG GAGTTCCATC AGAGTCTACA GTGGATGAAA 
GACAATGACA TCACAGACAT GCTGGACCTT ACCTTCACTG TGAATGAGGA AGTCTTCGGA 
CAGGTAACAG AGAGAGAGCT GAAGTCTGGA GGGGCCAACA TTAATGTGAC AGAGAAGAAT 
AAGAAGGAGT ACATTGACCG CATGGTGAAA TGGAGGGTGG AGAGAGGAGT GGTCAAGCAG 
ACAGAGGCAC TGGTCAGGGG CTTCTATGAG GTGGTGGATT CCCGGCTGGT GTCAGTGTTC 
GATGCCAGAG AGTTGGAGCT GGTCATCGCT GGGACAGCTG AGATAGACCT CAATGACTGG 
AGGAACAACA CAGAGTATAG AGGAGGCTAC CATGACAGCC ACATAGTGAT CCGGTGGTTC 
TGGGGGGCCG TGGAGCGCTT CAACAATGAG CAGAGGCTGC GTCTGCTGCA GTTTGTGACT 
GGCACCTCTA GTGTTCCCTA CGAGGGCTTC ACTGCGCTAC GGGGCAGTAA TGGCCTGCGA 
CGCTTCTGTA TCGAGAAGTG GGGCAAGATC ACCGCTCTTC CAAGGGCACA CACATGCTTC 
AACCGGCTGG ATCTCCCTCC ATACCCCTCC TACACCATGC TCTATGAGAA ACTGCTGATC 
GCTGTGGAGG AGACCAGCAC TTTTGGCCTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014045925.1
CDS	212..4987
Translation	MIQRQVTLTQNLYQNRFLGLRLAAMASPCRSTQNRRRCKDPIRHSYNPEQFRNVNFQNGVPEDVAAMYRSTSDTDLVTSHSRSTLTVSTSLYAIGQTQDILICWDIKEEVDAGDWIGMYLIEEVSSENFLDYKSRGVNGSHKGQIVWKIEGNSYFVEPETKICFKYYHGVSGALRATTPSLTVKNPSVPVFKPVANEEVPQSTGSRRLISFSLSDFHATGLKKGMFFNPDPYLKIAIHPGRNSIFPALPHHGQEKRSGIVCNTINPHWQRERFNFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKQSMPVQRLLEKHAIGDRVVSYTLGRRVPTEHVSGQLQFRFEITSSIHPDDEDMSLSTEPECAAMQETSNQGEGQTSQAQGQAKDTMSLGPGAPELPMDEEEDAGAPELPIGGDTVTDTPMEGHPAGPLGSEQPMDGDGDGDSGSSCSRAKALKTEPSQPEVNGAQGKEEVAVAVAVAAEKLPTVEDNREEGGQQTPSSPQLADPEQGVDSTPAEGQVIPAVLEDMESGAPGEPAEQVVGQEEGDEECCSLRIRTTPRRKNRPCSLPVSELETVIASACGEPETPRSHYIRIHHLLHSLPSTQPGTQEDGEGDGAEAETEPDPDPGPDQLSPRVQDGSEEDEKAEPLTPGPRRTLPRSLSIERLSDLALLLDSEGRHPQGQVRGHQRVSDEDDMEPCCSDSCYEGRGGRHNQAPLVAAARMSVESSDFPSQEDEDMEVNGAESREPGDGMPQWQVTPSTQVQSPQSFIKNGESPVAGPSGKIGRDCPLPSSHPPVSQLPALRHDPHHYPTIDEPLPPNWEARVDSHGRVFYVDHINRTTTWQRPTSAATPDGMRRSGSVQQMEQLNRRYQSIQRTMATDRSENEGSRSRSERTSLNNNSSETDSPSMANELRRDSSSSSSSSSSSPVNSQKITAMLQSPAVKFITHPEFFTVLHANYGAYRMFTSSTCLKHMILKIRRDARNLERYQHNKDLVTFLNRFADAQLELPRGWEIKTDPQGKNFFVDHNSRATTFIDPRIPLQNGCLPNHLTHRQHLQRLRSYSAGEASEVSRSRGAALLSRPGNSVVAAMRSQHHPDPVPQAYNEKIVAFLRQPGILEVLTERQPALSRNHSLREKIHYIRTEGTERLEKLACDADLVILLSLFEEEIMSYVPPSFHPGFSFSPRCSPGSSPQNSPGMSRARAPAPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIVRRDHLLEGTFNQVMAYSRKELQRNKLYITFIGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLDWFRFSGRILGLVLIHQYLLDAFFTRPFYKALLRLATDLSDLEYLDEEFHQSLQWMKDNDITDMLDLTFTVNEEVFGQVTERELKSGGANINVTEKNKKEYIDRMVKWRVERGVVKQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDSHIVIRWFWGAVERFNNEQRLRLLQFVTGTSSVPYEGFTALRGSNGLRRFCIEKWGKITALPRAHTCFNRLDLPPYPSYTMLYEKLLIAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_014190450.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar E3 ubiquitin-protein ligase HECW1-like (LOC106599291), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014190450.1

ORF Insert Sequence:

ATGATTCAAA GGCAGGTGAC ACTGACCCAG AACTTGTACC AGAACAGGTT TCTCGGACTC 
AGACTTGCAG CCATGGCCTC CCCATGTCGT TCAACCCAGA ACCGTCGGCG CTGTAAGGAC 
CCCATCCGCC ACAGCTACAA CCCCGAACAA TTTCGCAACG TTAATTTCCA GAATGGGGTG 
CCGGAGGACG TGGCAGCCAT GTACCGCTCC ACCAGCGACA CGGACCTGGT GACATCACAC 
AGCCGCTCCA CGCTTACCGT CAGCACCTCG CTCTACGCCA TCGGACAGAC CCAGGACATC 
CTCATCTGCT GGGACATCAA GGAGGAGGTG GACGCTGGGG ACTGGATCGG CATGTACCTC 
ATTGAAGAGG TGTCGTCGGA GAACTTTCTG GACTACAAGA GCCGCGGAGT GAATGGTTCC 
CACAAGGGCC AGATAGTGTG GAAGATTGAG GGCAACTCCT ATTTTGTGGA GCCGGAGACT 
AAGATCTGTT TTAAATATTA CCATGGTGTG AGCGGGGCCC TGCGAGCCAC TACCCCCAGT 
TTGACTGTGA AGAACCCCTC AGTTCCGGTA TTTAAGCCCG TCGCCAATGA AGAAGTCCCC 
CAGAGCACAG GCAGTAGAAG ACTCATCAGC TTCTCCCTCT CAGATTTCCA TGCAACAGGG 
CTGAAGAAGG GGATGTTCTT CAACCCTGAC CCCTACCTTA AGATCGCCAT CCACCCCGGC 
AGGAACAGCA TCTTCCCGGC ACTGCCACAC CACGGCCAGG AGAAACGCTC AGGCATCGTC 
TGTAACACCA TCAACCCCCA CTGGCAGAGA GAGCGCTTCA ACTTTGTGTC TCTGCCCACG 
GACGTTCTGG AGATCGAGGT AAAAGACAAG TTTGCCAAGA GTCGTCCTAT CATCAAACGT 
TTCCTGGGCA AGCAGTCCAT GCCGGTGCAG AGGCTACTGG AGAAGCATGC TATTGGTGAC 
CGTGTGGTCA GCTACACGCT GGGCCGAAGG GTGCCGACGG AACATGTCAG TGGACAGCTG 
CAGTTCAGAT TTGAGATCAC TTCCTCTATT CATCCAGATG ATGAAGACAT GTCTCTCAGC 
ACAGAGCCTG AGTGTGCCGC TATGCAGGAG ACCAGTAACC AGGGGGAGGG CCAGACCTCC 
CAGGCCCAGG GCCAGGCCAA GGACACCATG AGCCTGGGTC CGGGGGCTCC CGAACTCCCC 
ATGGATGAGG AGGAAGACGC TGGAGCTCCA GAACTTCCAA TTGGAGGAGA TACAGTTACA 
GACACGCCCA TGGAGGGGCA TCCAGCAGGG CCTTTGGGTT CAGAGCAGCC CATGGATGGA 
GATGGAGATG GAGACTCAGG GTCTAGCTGT TCCAGAGCCA AGGCCCTAAA AACAGAACCA 
AGCCAACCAG AGGTCAATGG AGCTCAGGGG AAGGAGGAGG TTGCTGTGGC TGTAGCTGTG 
GCTGCAGAGA AGCTGCCCAC GGTAGAGGAT AACAGGGAGG AAGGGGGGCA GCAAACCCCC 
TCCAGTCCTC AGCTTGCTGA CCCAGAGCAG GGGGTGGACA GTACCCCAGC AGAGGGTCAG 
GTCATCCCTG CTGTATTGGA AGACATGGAG AGTGGAGCGC CTGGGGAGCC AGCAGAACAG 
GTGGTGGGTC AGGAGGAAGG GGACGAGGAA TGCTGCTCCC TGCGCATCAG GACCACCCCT 
AGGCGTAAGA ACCGGCCCTG CTCCCTGCCC GTGTCAGAGC TGGAGACAGT CATCGCCTCG 
GCCTGTGGGG AGCCCGAGAC CCCTCGCTCC CACTACATCC GCATCCACCA CCTCCTCCAC 
AGTCTGCCCT CCACCCAGCC AGGCACCCAG GAGGACGGGG AGGGAGATGG GGCTGAGGCT 
GAAACAGAGC CCGATCCAGA CCCAGGCCCT GACCAGCTCA GCCCCAGGGT CCAGGATGGC 
TCAGAAGAGG ATGAAAAGGC AGAACCCCTG ACCCCCGGGC CCAGGAGGAC CCTGCCTCGC 
AGCCTGTCCA TCGAGCGTCT GTCCGACCTG GCCCTGCTAC TGGACAGCGA GGGCCGCCAC 
CCCCAGGGCC AGGTCAGGGG TCACCAGAGA GTGTCCGACG AGGATGACAT GGAGCCCTGC 
TGCAGTGATT CCTGCTATGA GGGCCGAGGA GGCCGTCACA ACCAGGCACC TCTAGTGGCT 
GCAGCCAGGA TGTCTGTGGA GAGCAGTGAT TTCCCCTCCC AGGAGGATGA GGACATGGAG 
GTGAACGGAG CGGAGAGCAG GGAGCCAGGA GATGGGATGC CCCAGTGGCA GGTGACCCCG 
TCCACGCAGG TCCAGAGCCC CCAGAGCTTC ATAAAAAACG GAGAGTCACC CGTGGCCGGG 
CCCAGTGGGA AGATAGGACG AGATTGTCCC CTACCGTCCA GTCATCCTCC AGTCAGCCAG 
CTTCCTGCTC TGCGTCATGA TCCTCATCAT TACCCCACTA TAGATGAACC ACTTCCACCA 
AACTGGGAGG CGCGTGTGGA CAGCCACGGT CGTGTGTTCT ACGTGGACCA CATCAACAGG 
ACCACCACGT GGCAGAGACC CACCTCAGCA GCCACGCCCG ACGGCATGAG GAGGTCCGGA 
TCCGTTCAAC AGATGGAACA GCTCAATAGA AGGTACCAGA GCATCCAGAG GACCATGGCC 
ACAGACAGGA GTGAGAATGA AGGAAGCAGA AGCAGGAGTG AGAGAACAAG CCTCAACAAC 
AACAGCAGTG AGACAGACTC TCCTTCGATG GCTAATGAGC TGAGAAGAGA CAGCTCCTCT 
TCCTCCTCCT CCTCGTCATC ATCTCCAGTC AACAGTCAGA AGATCACAGC TATGTTACAG 
AGTCCAGCGG TGAAGTTCAT CACACACCCA GAGTTTTTCA CTGTCCTCCA TGCCAACTAT 
GGGGCCTACC GCATGTTCAC CAGCAGCACG TGCCTGAAGC ACATGATCCT GAAGATTCGT 
AGAGACGCTC GTAACCTTGA GAGGTACCAG CACAACAAAG ACCTGGTTAC CTTCCTCAAC 
CGCTTCGCTG ACGCCCAGCT GGAACTGCCT CGGGGCTGGG AGATCAAGAC TGACCCTCAG 
GGCAAGAACT TCTTTGTGGA CCACAACAGC CGAGCAACCA CGTTCATCGA CCCGAGAATC 
CCTCTTCAGA ACGGTTGTCT GCCCAACCAC CTGACCCACA GGCAGCACCT GCAGCGATTA 
CGCAGCTACA GTGCTGGAGA GGCCTCGGAG GTGTCCCGTA GTCGAGGTGC GGCCTTGTTG 
TCACGGCCTG GGAACAGTGT GGTGGCCGCG ATGCGGAGCC AGCATCATCC TGATCCAGTG 
CCACAAGCGT ATAATGAGAA GATAGTGGCC TTCCTCCGTC AGCCTGGAAT ACTAGAAGTT 
CTAACGGAGC GCCAGCCAGC CCTGTCCAGG AACCATTCCC TGAGGGAAAA GATCCACTAC 
ATCAGAACAG AGGGCACCGA AAGACTGGAG AAACTAGCAT GTGATGCAGA CCTGGTGATA 
CTGTTGAGCT TGTTTGAAGA GGAGATCATG TCCTATGTTC CGCCCTCCTT CCATCCTGGC 
TTCAGCTTCT CTCCGCGTTG TTCACCAGGG TCTTCACCAC AGAACTCTCC AGGAATGTCG 
AGAGCGAGAG CACCTGCCCC GTACCGGAGA GACTTTGAGG CCAAACTACG CAACTTCTAC 
AGGAAACTGG AGGCTAAAGG CTATGGCCAG GGCCCAGGAA AGATCAAGCT TATCGTCCGG 
AGAGACCACC TGCTGGAGGG TACCTTTAAC CAGGTCATGG CCTACTCACG CAAGGAACTA 
CAGAGGAATA AGCTCTACAT CACTTTCATA GGAGAGGAGG GGCTGGACTA CAGTGGGCCA 
TCCAGAGAGT TCTTCTTCCT GCTGTCTCAG GAGCTGTTCA ACCCCTACTA TGGCCTGTTT 
GAGTACTCAG CCAACGACAC ATACACCGTC CAGATCAGCC CCATGTCCGC CTTCGTAGAG 
AACCATCTGG ATTGGTTCCG GTTTAGTGGG CGTATTTTGG GACTGGTGTT GATCCACCAG 
TATCTCCTGG ATGCCTTCTT CACGCGGCCC TTCTACAAGG CTCTCCTTAG ACTAGCCACT 
GACCTGAGTG ATCTGGAATA CCTAGACGAG GAGTTCCATC AGAGTCTACA GTGGATGAAA 
GACAATGACA TCACAGACAT GCTGGACCTT ACCTTCACTG TGAATGAGGA AGTCTTCGGA 
CAGGTAACAG AGAGAGAGCT GAAGTCTGGA GGGGCCAACA TTAATGTGAC AGAGAAGAAT 
AAGAAGGAGT ACATTGACCG CATGGTGAAA TGGAGGGTGG AGAGAGGAGT GGTCAAGCAG 
ACAGAGGCAC TGGTCAGGGG CTTCTATGAG GTGGTGGATT CCCGGCTGGT GTCAGTGTTC 
GATGCCAGAG AGTTGGAGCT GGTCATCGCT GGGACAGCTG AGATAGACCT CAATGACTGG 
AGGAACAACA CAGAGTATAG AGGAGGCTAC CATGACAGCC ACATAGTGAT CCGGTGGTTC 
TGGGGGGCCG TGGAGCGCTT CAACAATGAG CAGAGGCTGC GTCTGCTGCA GTTTGTGACT 
GGCACCTCTA GTGTTCCCTA CGAGGGCTTC ACTGCGCTAC GGGGCAGTAA TGGCCTGCGA 
CGCTTCTGTA TCGAGAAGTG GGGCAAGATC ACCGCTCTTC CAAGGGCACA CACATGCTTC 
AACCGGCTGG ATCTCCCTCC ATACCCCTCC TACACCATGC TCTATGAGAA ACTGCTGATC 
GCTGTGGAGG AGACCAGCAC TTTTGGCCTT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106599291 ( XM_014190451.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_014045926.1 Salmo salar E3 ubiquitin-protein ligase HECW1-like (LOC106599291), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAt11995
Clone ID Related Accession (Same CDS sequence)	XM_014190451.1
Accession Version	XM_014190451.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	E3 ubiquitin-protein ligase HECW1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012333553.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCAAA GGCAGGTGAC ACTGACCCAG AACTTGTACC AGAACAGGTT TCTCGGACTC 
AGACTTGCAG CCATGGCCTC CCCATGTCGT TCAACCCAGA ACCGTCGGCG CTGTAAGGAC 
CCCATCCGCC ACAGCTACAA CCCCGAACAA TTTCGCAACG TTAATTTCCA GAATGGGGTG 
CCGGAGGACG TGGCAGCCAT GTACCGCTCC ACCAGCGACA CGGACCTGGT GACATCACAC 
AGCCGCTCCA CGCTTACCGT CAGCACCTCG CTCTACGCCA TCGGACAGAC CCAGGACATC 
CTCATCTGCT GGGACATCAA GGAGGAGGTG GACGCTGGGG ACTGGATCGG CATGTACCTC 
ATTGAGGTGT CGTCGGAGAA CTTTCTGGAC TACAAGAGCC GCGGAGTGAA TGGTTCCCAC 
AAGGGCCAGA TAGTGTGGAA GATTGAGGGC AACTCCTATT TTGTGGAGCC GGAGACTAAG 
ATCTGTTTTA AATATTACCA TGGTGTGAGC GGGGCCCTGC GAGCCACTAC CCCCAGTTTG 
ACTGTGAAGA ACCCCTCAGT TCCGGTATTT AAGCCCGTCG CCAATGAAGA AGTCCCCCAG 
AGCACAGGCA GTAGAAGACT CATCAGCTTC TCCCTCTCAG ATTTCCATGC AACAGGGCTG 
AAGAAGGGGA TGTTCTTCAA CCCTGACCCC TACCTTAAGA TCGCCATCCA CCCCGGCAGG 
AACAGCATCT TCCCGGCACT GCCACACCAC GGCCAGGAGA AACGCTCAGG CATCGTCTGT 
AACACCATCA ACCCCCACTG GCAGAGAGAG CGCTTCAACT TTGTGTCTCT GCCCACGGAC 
GTTCTGGAGA TCGAGGTAAA AGACAAGTTT GCCAAGAGTC GTCCTATCAT CAAACGTTTC 
CTGGGCAAGC AGTCCATGCC GGTGCAGAGG CTACTGGAGA AGCATGCTAT TGGTGACCGT 
GTGGTCAGCT ACACGCTGGG CCGAAGGGTG CCGACGGAAC ATGTCAGTGG ACAGCTGCAG 
TTCAGATTTG AGATCACTTC CTCTATTCAT CCAGATGATG AAGACATGTC TCTCAGCACA 
GAGCCTGAGT GTGCCGCTAT GCAGGAGACC AGTAACCAGG GGGAGGGCCA GACCTCCCAG 
GCCCAGGGCC AGGCCAAGGA CACCATGAGC CTGGGTCCGG GGGCTCCCGA ACTCCCCATG 
GATGAGGAGG AAGACGCTGG AGCTCCAGAA CTTCCAATTG GAGGAGATAC AGTTACAGAC 
ACGCCCATGG AGGGGCATCC AGCAGGGCCT TTGGGTTCAG AGCAGCCCAT GGATGGAGAT 
GGAGATGGAG ACTCAGGGTC TAGCTGTTCC AGAGCCAAGG CCCTAAAAAC AGAACCAAGC 
CAACCAGAGG TCAATGGAGC TCAGGGGAAG GAGGAGGTTG CTGTGGCTGT AGCTGTGGCT 
GCAGAGAAGC TGCCCACGGT AGAGGATAAC AGGGAGGAAG GGGGGCAGCA AACCCCCTCC 
AGTCCTCAGC TTGCTGACCC AGAGCAGGGG GTGGACAGTA CCCCAGCAGA GGGTCAGGTC 
ATCCCTGCTG TATTGGAAGA CATGGAGAGT GGAGCGCCTG GGGAGCCAGC AGAACAGGTG 
GTGGGTCAGG AGGAAGGGGA CGAGGAATGC TGCTCCCTGC GCATCAGGAC CACCCCTAGG 
CGTAAGAACC GGCCCTGCTC CCTGCCCGTG TCAGAGCTGG AGACAGTCAT CGCCTCGGCC 
TGTGGGGAGC CCGAGACCCC TCGCTCCCAC TACATCCGCA TCCACCACCT CCTCCACAGT 
CTGCCCTCCA CCCAGCCAGG CACCCAGGAG GACGGGGAGG GAGATGGGGC TGAGGCTGAA 
ACAGAGCCCG ATCCAGACCC AGGCCCTGAC CAGCTCAGCC CCAGGGTCCA GGATGGCTCA 
GAAGAGGATG AAAAGGCAGA ACCCCTGACC CCCGGGCCCA GGAGGACCCT GCCTCGCAGC 
CTGTCCATCG AGCGTCTGTC CGACCTGGCC CTGCTACTGG ACAGCGAGGG CCGCCACCCC 
CAGGGCCAGG TCAGGGGTCA CCAGAGAGTG TCCGACGAGG ATGACATGGA GCCCTGCTGC 
AGTGATTCCT GCTATGAGGG CCGAGGAGGC CGTCACAACC AGGCACCTCT AGTGGCTGCA 
GCCAGGATGT CTGTGGAGAG CAGTGATTTC CCCTCCCAGG AGGATGAGGA CATGGAGGTG 
AACGGAGCGG AGAGCAGGGA GCCAGGAGAT GGGATGCCCC AGTGGCAGGT GACCCCGTCC 
ACGCAGGTCC AGAGCCCCCA GAGCTTCATA AAAAACGGAG AGTCACCCGT GGCCGGGCCC 
AGTGGGAAGA TAGGACGAGA TTGTCCCCTA CCGTCCAGTC ATCCTCCAGT CAGCCAGCTT 
CCTGCTCTGC GTCATGATCC TCATCATTAC CCCACTATAG ATGAACCACT TCCACCAAAC 
TGGGAGGCGC GTGTGGACAG CCACGGTCGT GTGTTCTACG TGGACCACAT CAACAGGACC 
ACCACGTGGC AGAGACCCAC CTCAGCAGCC ACGCCCGACG GCATGAGGAG GTCCGGATCC 
GTTCAACAGA TGGAACAGCT CAATAGAAGG TACCAGAGCA TCCAGAGGAC CATGGCCACA 
GACAGGAGTG AGAATGAAGG AAGCAGAAGC AGGAGTGAGA GAACAAGCCT CAACAACAAC 
AGCAGTGAGA CAGACTCTCC TTCGATGGCT AATGAGCTGA GAAGAGACAG CTCCTCTTCC 
TCCTCCTCCT CGTCATCATC TCCAGTCAAC AGTCAGAAGA TCACAGCTAT GTTACAGAGT 
CCAGCGGTGA AGTTCATCAC ACACCCAGAG TTTTTCACTG TCCTCCATGC CAACTATGGG 
GCCTACCGCA TGTTCACCAG CAGCACGTGC CTGAAGCACA TGATCCTGAA GATTCGTAGA 
GACGCTCGTA ACCTTGAGAG GTACCAGCAC AACAAAGACC TGGTTACCTT CCTCAACCGC 
TTCGCTGACG CCCAGCTGGA ACTGCCTCGG GGCTGGGAGA TCAAGACTGA CCCTCAGGGC 
AAGAACTTCT TTGTGGACCA CAACAGCCGA GCAACCACGT TCATCGACCC GAGAATCCCT 
CTTCAGAACG GTTGTCTGCC CAACCACCTG ACCCACAGGC AGCACCTGCA GCGATTACGC 
AGCTACAGTG CTGGAGAGGC CTCGGAGGTG TCCCGTAGTC GAGGTGCGGC CTTGTTGTCA 
CGGCCTGGGA ACAGTGTGGT GGCCGCGATG CGGAGCCAGC ATCATCCTGA TCCAGTGCCA 
CAAGCGTATA ATGAGAAGAT AGTGGCCTTC CTCCGTCAGC CTGGAATACT AGAAGTTCTA 
ACGGAGCGCC AGCCAGCCCT GTCCAGGAAC CATTCCCTGA GGGAAAAGAT CCACTACATC 
AGAACAGAGG GCACCGAAAG ACTGGAGAAA CTAGCATGTG ATGCAGACCT GGTGATACTG 
TTGAGCTTGT TTGAAGAGGA GATCATGTCC TATGTTCCGC CCTCCTTCCA TCCTGGCTTC 
AGCTTCTCTC CGCGTTGTTC ACCAGGGTCT TCACCACAGA ACTCTCCAGG AATGTCGAGA 
GCGAGAGCAC CTGCCCCGTA CCGGAGAGAC TTTGAGGCCA AACTACGCAA CTTCTACAGG 
AAACTGGAGG CTAAAGGCTA TGGCCAGGGC CCAGGAAAGA TCAAGCTTAT CGTCCGGAGA 
GACCACCTGC TGGAGGGTAC CTTTAACCAG GTCATGGCCT ACTCACGCAA GGAACTACAG 
AGGAATAAGC TCTACATCAC TTTCATAGGA GAGGAGGGGC TGGACTACAG TGGGCCATCC 
AGAGAGTTCT TCTTCCTGCT GTCTCAGGAG CTGTTCAACC CCTACTATGG CCTGTTTGAG 
TACTCAGCCA ACGACACATA CACCGTCCAG ATCAGCCCCA TGTCCGCCTT CGTAGAGAAC 
CATCTGGATT GGTTCCGGTT TAGTGGGCGT ATTTTGGGAC TGGTGTTGAT CCACCAGTAT 
CTCCTGGATG CCTTCTTCAC GCGGCCCTTC TACAAGGCTC TCCTTAGACT AGCCACTGAC 
CTGAGTGATC TGGAATACCT AGACGAGGAG TTCCATCAGA GTCTACAGTG GATGAAAGAC 
AATGACATCA CAGACATGCT GGACCTTACC TTCACTGTGA ATGAGGAAGT CTTCGGACAG 
GTAACAGAGA GAGAGCTGAA GTCTGGAGGG GCCAACATTA ATGTGACAGA GAAGAATAAG 
AAGGAGTACA TTGACCGCAT GGTGAAATGG AGGGTGGAGA GAGGAGTGGT CAAGCAGACA 
GAGGCACTGG TCAGGGGCTT CTATGAGGTG GTGGATTCCC GGCTGGTGTC AGTGTTCGAT 
GCCAGAGAGT TGGAGCTGGT CATCGCTGGG ACAGCTGAGA TAGACCTCAA TGACTGGAGG 
AACAACACAG AGTATAGAGG AGGCTACCAT GACAGCCACA TAGTGATCCG GTGGTTCTGG 
GGGGCCGTGG AGCGCTTCAA CAATGAGCAG AGGCTGCGTC TGCTGCAGTT TGTGACTGGC 
ACCTCTAGTG TTCCCTACGA GGGCTTCACT GCGCTACGGG GCAGTAATGG CCTGCGACGC 
TTCTGTATCG AGAAGTGGGG CAAGATCACC GCTCTTCCAA GGGCACACAC ATGCTTCAAC 
CGGCTGGATC TCCCTCCATA CCCCTCCTAC ACCATGCTCT ATGAGAAACT GCTGATCGCT 
GTGGAGGAGA CCAGCACTTT TGGCCTTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014045926.1
CDS	213..4985
Translation	MIQRQVTLTQNLYQNRFLGLRLAAMASPCRSTQNRRRCKDPIRHSYNPEQFRNVNFQNGVPEDVAAMYRSTSDTDLVTSHSRSTLTVSTSLYAIGQTQDILICWDIKEEVDAGDWIGMYLIEVSSENFLDYKSRGVNGSHKGQIVWKIEGNSYFVEPETKICFKYYHGVSGALRATTPSLTVKNPSVPVFKPVANEEVPQSTGSRRLISFSLSDFHATGLKKGMFFNPDPYLKIAIHPGRNSIFPALPHHGQEKRSGIVCNTINPHWQRERFNFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKQSMPVQRLLEKHAIGDRVVSYTLGRRVPTEHVSGQLQFRFEITSSIHPDDEDMSLSTEPECAAMQETSNQGEGQTSQAQGQAKDTMSLGPGAPELPMDEEEDAGAPELPIGGDTVTDTPMEGHPAGPLGSEQPMDGDGDGDSGSSCSRAKALKTEPSQPEVNGAQGKEEVAVAVAVAAEKLPTVEDNREEGGQQTPSSPQLADPEQGVDSTPAEGQVIPAVLEDMESGAPGEPAEQVVGQEEGDEECCSLRIRTTPRRKNRPCSLPVSELETVIASACGEPETPRSHYIRIHHLLHSLPSTQPGTQEDGEGDGAEAETEPDPDPGPDQLSPRVQDGSEEDEKAEPLTPGPRRTLPRSLSIERLSDLALLLDSEGRHPQGQVRGHQRVSDEDDMEPCCSDSCYEGRGGRHNQAPLVAAARMSVESSDFPSQEDEDMEVNGAESREPGDGMPQWQVTPSTQVQSPQSFIKNGESPVAGPSGKIGRDCPLPSSHPPVSQLPALRHDPHHYPTIDEPLPPNWEARVDSHGRVFYVDHINRTTTWQRPTSAATPDGMRRSGSVQQMEQLNRRYQSIQRTMATDRSENEGSRSRSERTSLNNNSSETDSPSMANELRRDSSSSSSSSSSSPVNSQKITAMLQSPAVKFITHPEFFTVLHANYGAYRMFTSSTCLKHMILKIRRDARNLERYQHNKDLVTFLNRFADAQLELPRGWEIKTDPQGKNFFVDHNSRATTFIDPRIPLQNGCLPNHLTHRQHLQRLRSYSAGEASEVSRSRGAALLSRPGNSVVAAMRSQHHPDPVPQAYNEKIVAFLRQPGILEVLTERQPALSRNHSLREKIHYIRTEGTERLEKLACDADLVILLSLFEEEIMSYVPPSFHPGFSFSPRCSPGSSPQNSPGMSRARAPAPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIVRRDHLLEGTFNQVMAYSRKELQRNKLYITFIGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLDWFRFSGRILGLVLIHQYLLDAFFTRPFYKALLRLATDLSDLEYLDEEFHQSLQWMKDNDITDMLDLTFTVNEEVFGQVTERELKSGGANINVTEKNKKEYIDRMVKWRVERGVVKQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDSHIVIRWFWGAVERFNNEQRLRLLQFVTGTSSVPYEGFTALRGSNGLRRFCIEKWGKITALPRAHTCFNRLDLPPYPSYTMLYEKLLIAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_014190451.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar E3 ubiquitin-protein ligase HECW1-like (LOC106599291), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014190451.1

ORF Insert Sequence:

ATGATTCAAA GGCAGGTGAC ACTGACCCAG AACTTGTACC AGAACAGGTT TCTCGGACTC 
AGACTTGCAG CCATGGCCTC CCCATGTCGT TCAACCCAGA ACCGTCGGCG CTGTAAGGAC 
CCCATCCGCC ACAGCTACAA CCCCGAACAA TTTCGCAACG TTAATTTCCA GAATGGGGTG 
CCGGAGGACG TGGCAGCCAT GTACCGCTCC ACCAGCGACA CGGACCTGGT GACATCACAC 
AGCCGCTCCA CGCTTACCGT CAGCACCTCG CTCTACGCCA TCGGACAGAC CCAGGACATC 
CTCATCTGCT GGGACATCAA GGAGGAGGTG GACGCTGGGG ACTGGATCGG CATGTACCTC 
ATTGAGGTGT CGTCGGAGAA CTTTCTGGAC TACAAGAGCC GCGGAGTGAA TGGTTCCCAC 
AAGGGCCAGA TAGTGTGGAA GATTGAGGGC AACTCCTATT TTGTGGAGCC GGAGACTAAG 
ATCTGTTTTA AATATTACCA TGGTGTGAGC GGGGCCCTGC GAGCCACTAC CCCCAGTTTG 
ACTGTGAAGA ACCCCTCAGT TCCGGTATTT AAGCCCGTCG CCAATGAAGA AGTCCCCCAG 
AGCACAGGCA GTAGAAGACT CATCAGCTTC TCCCTCTCAG ATTTCCATGC AACAGGGCTG 
AAGAAGGGGA TGTTCTTCAA CCCTGACCCC TACCTTAAGA TCGCCATCCA CCCCGGCAGG 
AACAGCATCT TCCCGGCACT GCCACACCAC GGCCAGGAGA AACGCTCAGG CATCGTCTGT 
AACACCATCA ACCCCCACTG GCAGAGAGAG CGCTTCAACT TTGTGTCTCT GCCCACGGAC 
GTTCTGGAGA TCGAGGTAAA AGACAAGTTT GCCAAGAGTC GTCCTATCAT CAAACGTTTC 
CTGGGCAAGC AGTCCATGCC GGTGCAGAGG CTACTGGAGA AGCATGCTAT TGGTGACCGT 
GTGGTCAGCT ACACGCTGGG CCGAAGGGTG CCGACGGAAC ATGTCAGTGG ACAGCTGCAG 
TTCAGATTTG AGATCACTTC CTCTATTCAT CCAGATGATG AAGACATGTC TCTCAGCACA 
GAGCCTGAGT GTGCCGCTAT GCAGGAGACC AGTAACCAGG GGGAGGGCCA GACCTCCCAG 
GCCCAGGGCC AGGCCAAGGA CACCATGAGC CTGGGTCCGG GGGCTCCCGA ACTCCCCATG 
GATGAGGAGG AAGACGCTGG AGCTCCAGAA CTTCCAATTG GAGGAGATAC AGTTACAGAC 
ACGCCCATGG AGGGGCATCC AGCAGGGCCT TTGGGTTCAG AGCAGCCCAT GGATGGAGAT 
GGAGATGGAG ACTCAGGGTC TAGCTGTTCC AGAGCCAAGG CCCTAAAAAC AGAACCAAGC 
CAACCAGAGG TCAATGGAGC TCAGGGGAAG GAGGAGGTTG CTGTGGCTGT AGCTGTGGCT 
GCAGAGAAGC TGCCCACGGT AGAGGATAAC AGGGAGGAAG GGGGGCAGCA AACCCCCTCC 
AGTCCTCAGC TTGCTGACCC AGAGCAGGGG GTGGACAGTA CCCCAGCAGA GGGTCAGGTC 
ATCCCTGCTG TATTGGAAGA CATGGAGAGT GGAGCGCCTG GGGAGCCAGC AGAACAGGTG 
GTGGGTCAGG AGGAAGGGGA CGAGGAATGC TGCTCCCTGC GCATCAGGAC CACCCCTAGG 
CGTAAGAACC GGCCCTGCTC CCTGCCCGTG TCAGAGCTGG AGACAGTCAT CGCCTCGGCC 
TGTGGGGAGC CCGAGACCCC TCGCTCCCAC TACATCCGCA TCCACCACCT CCTCCACAGT 
CTGCCCTCCA CCCAGCCAGG CACCCAGGAG GACGGGGAGG GAGATGGGGC TGAGGCTGAA 
ACAGAGCCCG ATCCAGACCC AGGCCCTGAC CAGCTCAGCC CCAGGGTCCA GGATGGCTCA 
GAAGAGGATG AAAAGGCAGA ACCCCTGACC CCCGGGCCCA GGAGGACCCT GCCTCGCAGC 
CTGTCCATCG AGCGTCTGTC CGACCTGGCC CTGCTACTGG ACAGCGAGGG CCGCCACCCC 
CAGGGCCAGG TCAGGGGTCA CCAGAGAGTG TCCGACGAGG ATGACATGGA GCCCTGCTGC 
AGTGATTCCT GCTATGAGGG CCGAGGAGGC CGTCACAACC AGGCACCTCT AGTGGCTGCA 
GCCAGGATGT CTGTGGAGAG CAGTGATTTC CCCTCCCAGG AGGATGAGGA CATGGAGGTG 
AACGGAGCGG AGAGCAGGGA GCCAGGAGAT GGGATGCCCC AGTGGCAGGT GACCCCGTCC 
ACGCAGGTCC AGAGCCCCCA GAGCTTCATA AAAAACGGAG AGTCACCCGT GGCCGGGCCC 
AGTGGGAAGA TAGGACGAGA TTGTCCCCTA CCGTCCAGTC ATCCTCCAGT CAGCCAGCTT 
CCTGCTCTGC GTCATGATCC TCATCATTAC CCCACTATAG ATGAACCACT TCCACCAAAC 
TGGGAGGCGC GTGTGGACAG CCACGGTCGT GTGTTCTACG TGGACCACAT CAACAGGACC 
ACCACGTGGC AGAGACCCAC CTCAGCAGCC ACGCCCGACG GCATGAGGAG GTCCGGATCC 
GTTCAACAGA TGGAACAGCT CAATAGAAGG TACCAGAGCA TCCAGAGGAC CATGGCCACA 
GACAGGAGTG AGAATGAAGG AAGCAGAAGC AGGAGTGAGA GAACAAGCCT CAACAACAAC 
AGCAGTGAGA CAGACTCTCC TTCGATGGCT AATGAGCTGA GAAGAGACAG CTCCTCTTCC 
TCCTCCTCCT CGTCATCATC TCCAGTCAAC AGTCAGAAGA TCACAGCTAT GTTACAGAGT 
CCAGCGGTGA AGTTCATCAC ACACCCAGAG TTTTTCACTG TCCTCCATGC CAACTATGGG 
GCCTACCGCA TGTTCACCAG CAGCACGTGC CTGAAGCACA TGATCCTGAA GATTCGTAGA 
GACGCTCGTA ACCTTGAGAG GTACCAGCAC AACAAAGACC TGGTTACCTT CCTCAACCGC 
TTCGCTGACG CCCAGCTGGA ACTGCCTCGG GGCTGGGAGA TCAAGACTGA CCCTCAGGGC 
AAGAACTTCT TTGTGGACCA CAACAGCCGA GCAACCACGT TCATCGACCC GAGAATCCCT 
CTTCAGAACG GTTGTCTGCC CAACCACCTG ACCCACAGGC AGCACCTGCA GCGATTACGC 
AGCTACAGTG CTGGAGAGGC CTCGGAGGTG TCCCGTAGTC GAGGTGCGGC CTTGTTGTCA 
CGGCCTGGGA ACAGTGTGGT GGCCGCGATG CGGAGCCAGC ATCATCCTGA TCCAGTGCCA 
CAAGCGTATA ATGAGAAGAT AGTGGCCTTC CTCCGTCAGC CTGGAATACT AGAAGTTCTA 
ACGGAGCGCC AGCCAGCCCT GTCCAGGAAC CATTCCCTGA GGGAAAAGAT CCACTACATC 
AGAACAGAGG GCACCGAAAG ACTGGAGAAA CTAGCATGTG ATGCAGACCT GGTGATACTG 
TTGAGCTTGT TTGAAGAGGA GATCATGTCC TATGTTCCGC CCTCCTTCCA TCCTGGCTTC 
AGCTTCTCTC CGCGTTGTTC ACCAGGGTCT TCACCACAGA ACTCTCCAGG AATGTCGAGA 
GCGAGAGCAC CTGCCCCGTA CCGGAGAGAC TTTGAGGCCA AACTACGCAA CTTCTACAGG 
AAACTGGAGG CTAAAGGCTA TGGCCAGGGC CCAGGAAAGA TCAAGCTTAT CGTCCGGAGA 
GACCACCTGC TGGAGGGTAC CTTTAACCAG GTCATGGCCT ACTCACGCAA GGAACTACAG 
AGGAATAAGC TCTACATCAC TTTCATAGGA GAGGAGGGGC TGGACTACAG TGGGCCATCC 
AGAGAGTTCT TCTTCCTGCT GTCTCAGGAG CTGTTCAACC CCTACTATGG CCTGTTTGAG 
TACTCAGCCA ACGACACATA CACCGTCCAG ATCAGCCCCA TGTCCGCCTT CGTAGAGAAC 
CATCTGGATT GGTTCCGGTT TAGTGGGCGT ATTTTGGGAC TGGTGTTGAT CCACCAGTAT 
CTCCTGGATG CCTTCTTCAC GCGGCCCTTC TACAAGGCTC TCCTTAGACT AGCCACTGAC 
CTGAGTGATC TGGAATACCT AGACGAGGAG TTCCATCAGA GTCTACAGTG GATGAAAGAC 
AATGACATCA CAGACATGCT GGACCTTACC TTCACTGTGA ATGAGGAAGT CTTCGGACAG 
GTAACAGAGA GAGAGCTGAA GTCTGGAGGG GCCAACATTA ATGTGACAGA GAAGAATAAG 
AAGGAGTACA TTGACCGCAT GGTGAAATGG AGGGTGGAGA GAGGAGTGGT CAAGCAGACA 
GAGGCACTGG TCAGGGGCTT CTATGAGGTG GTGGATTCCC GGCTGGTGTC AGTGTTCGAT 
GCCAGAGAGT TGGAGCTGGT CATCGCTGGG ACAGCTGAGA TAGACCTCAA TGACTGGAGG 
AACAACACAG AGTATAGAGG AGGCTACCAT GACAGCCACA TAGTGATCCG GTGGTTCTGG 
GGGGCCGTGG AGCGCTTCAA CAATGAGCAG AGGCTGCGTC TGCTGCAGTT TGTGACTGGC 
ACCTCTAGTG TTCCCTACGA GGGCTTCACT GCGCTACGGG GCAGTAATGG CCTGCGACGC 
TTCTGTATCG AGAAGTGGGG CAAGATCACC GCTCTTCCAA GGGCACACAC ATGCTTCAAC 
CGGCTGGATC TCCCTCCATA CCCCTCCTAC ACCATGCTCT ATGAGAAACT GCTGATCGCT 
GTGGAGGAGA CCAGCACTTT TGGCCTTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106599291 ( XM_014190454.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_014045929.1 Salmo salar E3 ubiquitin-protein ligase HECW1-like (LOC106599291), transcript variant X3, mRNA.

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CloneID	OAt11996
Clone ID Related Accession (Same CDS sequence)	XM_014190454.1
Accession Version	XM_014190454.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	E3 ubiquitin-protein ligase HECW1-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012333553.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCCC CATGTCGTTC AACCCAGAAC CGTCGGCGCT GTAAGGACCC CATCCGCCAC 
AGCTACAACC CCGAACAATT TCGCAACGTT AATTTCCAGA ATGGGGTGCC GGAGGACGTG 
GCAGCCATGT ACCGCTCCAC CAGCGACACG GACCTGGTGA CATCACACAG CCGCTCCACG 
CTTACCGTCA GCACCTCGCT CTACGCCATC GGACAGACCC AGGACATCCT CATCTGCTGG 
GACATCAAGG AGGAGGTGGA CGCTGGGGAC TGGATCGGCA TGTACCTCAT TGAAGAGGTG 
TCGTCGGAGA ACTTTCTGGA CTACAAGAGC CGCGGAGTGA ATGGTTCCCA CAAGGGCCAG 
ATAGTGTGGA AGATTGAGGG CAACTCCTAT TTTGTGGAGC CGGAGACTAA GATCTGTTTT 
AAATATTACC ATGGTGTGAG CGGGGCCCTG CGAGCCACTA CCCCCAGTTT GACTGTGAAG 
AACCCCTCAG TTCCGGTATT TAAGCCCGTC GCCAATGAAG AAGTCCCCCA GAGCACAGGC 
AGTAGAAGAC TCATCAGCTT CTCCCTCTCA GATTTCCATG CAACAGGGCT GAAGAAGGGG 
ATGTTCTTCA ACCCTGACCC CTACCTTAAG ATCGCCATCC ACCCCGGCAG GAACAGCATC 
TTCCCGGCAC TGCCACACCA CGGCCAGGAG AAACGCTCAG GCATCGTCTG TAACACCATC 
AACCCCCACT GGCAGAGAGA GCGCTTCAAC TTTGTGTCTC TGCCCACGGA CGTTCTGGAG 
ATCGAGGTAA AAGACAAGTT TGCCAAGAGT CGTCCTATCA TCAAACGTTT CCTGGGCAAG 
CAGTCCATGC CGGTGCAGAG GCTACTGGAG AAGCATGCTA TTGGTGACCG TGTGGTCAGC 
TACACGCTGG GCCGAAGGGT GCCGACGGAA CATGTCAGTG GACAGCTGCA GTTCAGATTT 
GAGATCACTT CCTCTATTCA TCCAGATGAT GAAGACATGT CTCTCAGCAC AGAGCCTGAG 
TGTGCCGCTA TGCAGGAGAC CAGTAACCAG GGGGAGGGCC AGACCTCCCA GGCCCAGGGC 
CAGGCCAAGG ACACCATGAG CCTGGGTCCG GGGGCTCCCG AACTCCCCAT GGATGAGGAG 
GAAGACGCTG GAGCTCCAGA ACTTCCAATT GGAGGAGATA CAGTTACAGA CACGCCCATG 
GAGGGGCATC CAGCAGGGCC TTTGGGTTCA GAGCAGCCCA TGGATGGAGA TGGAGATGGA 
GACTCAGGGT CTAGCTGTTC CAGAGCCAAG GCCCTAAAAA CAGAACCAAG CCAACCAGAG 
GTCAATGGAG CTCAGGGGAA GGAGGAGGTT GCTGTGGCTG TAGCTGTGGC TGCAGAGAAG 
CTGCCCACGG TAGAGGATAA CAGGGAGGAA GGGGGGCAGC AAACCCCCTC CAGTCCTCAG 
CTTGCTGACC CAGAGCAGGG GGTGGACAGT ACCCCAGCAG AGGGTCAGGT CATCCCTGCT 
GTATTGGAAG ACATGGAGAG TGGAGCGCCT GGGGAGCCAG CAGAACAGGT GGTGGGTCAG 
GAGGAAGGGG ACGAGGAATG CTGCTCCCTG CGCATCAGGA CCACCCCTAG GCGTAAGAAC 
CGGCCCTGCT CCCTGCCCGT GTCAGAGCTG GAGACAGTCA TCGCCTCGGC CTGTGGGGAG 
CCCGAGACCC CTCGCTCCCA CTACATCCGC ATCCACCACC TCCTCCACAG TCTGCCCTCC 
ACCCAGCCAG GCACCCAGGA GGACGGGGAG GGAGATGGGG CTGAGGCTGA AACAGAGCCC 
GATCCAGACC CAGGCCCTGA CCAGCTCAGC CCCAGGGTCC AGGATGGCTC AGAAGAGGAT 
GAAAAGGCAG AACCCCTGAC CCCCGGGCCC AGGAGGACCC TGCCTCGCAG CCTGTCCATC 
GAGCGTCTGT CCGACCTGGC CCTGCTACTG GACAGCGAGG GCCGCCACCC CCAGGGCCAG 
GTCAGGGGTC ACCAGAGAGT GTCCGACGAG GATGACATGG AGCCCTGCTG CAGTGATTCC 
TGCTATGAGG GCCGAGGAGG CCGTCACAAC CAGGCACCTC TAGTGGCTGC AGCCAGGATG 
TCTGTGGAGA GCAGTGATTT CCCCTCCCAG GAGGATGAGG ACATGGAGGT GAACGGAGCG 
GAGAGCAGGG AGCCAGGAGA TGGGATGCCC CAGTGGCAGG TGACCCCGTC CACGCAGGTC 
CAGAGCCCCC AGAGCTTCAT AAAAAACGGA GAGTCACCCG TGGCCGGGCC CAGTGGGAAG 
ATAGGACGAG ATTGTCCCCT ACCGTCCAGT CATCCTCCAG TCAGCCAGCT TCCTGCTCTG 
CGTCATGATC CTCATCATTA CCCCACTATA GATGAACCAC TTCCACCAAA CTGGGAGGCG 
CGTGTGGACA GCCACGGTCG TGTGTTCTAC GTGGACCACA TCAACAGGAC CACCACGTGG 
CAGAGACCCA CCTCAGCAGC CACGCCCGAC GGCATGAGGA GGTCCGGATC CGTTCAACAG 
ATGGAACAGC TCAATAGAAG GTACCAGAGC ATCCAGAGGA CCATGGCCAC AGACAGGAGT 
GAGAATGAAG GAAGCAGAAG CAGGAGTGAG AGAACAAGCC TCAACAACAA CAGCAGTGAG 
ACAGACTCTC CTTCGATGGC TAATGAGCTG AGAAGAGACA GCTCCTCTTC CTCCTCCTCC 
TCGTCATCAT CTCCAGTCAA CAGTCAGAAG ATCACAGCTA TGTTACAGAG TCCAGCGGTG 
AAGTTCATCA CACACCCAGA GTTTTTCACT GTCCTCCATG CCAACTATGG GGCCTACCGC 
ATGTTCACCA GCAGCACGTG CCTGAAGCAC ATGATCCTGA AGATTCGTAG AGACGCTCGT 
AACCTTGAGA GGTACCAGCA CAACAAAGAC CTGGTTACCT TCCTCAACCG CTTCGCTGAC 
GCCCAGCTGG AACTGCCTCG GGGCTGGGAG ATCAAGACTG ACCCTCAGGG CAAGAACTTC 
TTTGTGGACC ACAACAGCCG AGCAACCACG TTCATCGACC CGAGAATCCC TCTTCAGAAC 
GGTTGTCTGC CCAACCACCT GACCCACAGG CAGCACCTGC AGCGATTACG CAGCTACAGT 
GCTGGAGAGG CCTCGGAGGT GTCCCGTAGT CGAGGTGCGG CCTTGTTGTC ACGGCCTGGG 
AACAGTGTGG TGGCCGCGAT GCGGAGCCAG CATCATCCTG ATCCAGTGCC ACAAGCGTAT 
AATGAGAAGA TAGTGGCCTT CCTCCGTCAG CCTGGAATAC TAGAAGTTCT AACGGAGCGC 
CAGCCAGCCC TGTCCAGGAA CCATTCCCTG AGGGAAAAGA TCCACTACAT CAGAACAGAG 
GGCACCGAAA GACTGGAGAA ACTAGCATGT GATGCAGACC TGGTGATACT GTTGAGCTTG 
TTTGAAGAGG AGATCATGTC CTATGTTCCG CCCTCCTTCC ATCCTGGCTT CAGCTTCTCT 
CCGCGTTGTT CACCAGGGTC TTCACCACAG AACTCTCCAG GAATGTCGAG AGCGAGAGCA 
CCTGCCCCGT ACCGGAGAGA CTTTGAGGCC AAACTACGCA ACTTCTACAG GAAACTGGAG 
GCTAAAGGCT ATGGCCAGGG CCCAGGAAAG ATCAAGCTTA TCGTCCGGAG AGACCACCTG 
CTGGAGGGTA CCTTTAACCA GGTCATGGCC TACTCACGCA AGGAACTACA GAGGAATAAG 
CTCTACATCA CTTTCATAGG AGAGGAGGGG CTGGACTACA GTGGGCCATC CAGAGAGTTC 
TTCTTCCTGC TGTCTCAGGA GCTGTTCAAC CCCTACTATG GCCTGTTTGA GTACTCAGCC 
AACGACACAT ACACCGTCCA GATCAGCCCC ATGTCCGCCT TCGTAGAGAA CCATCTGGAT 
TGGTTCCGGT TTAGTGGGCG TATTTTGGGA CTGGTGTTGA TCCACCAGTA TCTCCTGGAT 
GCCTTCTTCA CGCGGCCCTT CTACAAGGCT CTCCTTAGAC TAGCCACTGA CCTGAGTGAT 
CTGGAATACC TAGACGAGGA GTTCCATCAG AGTCTACAGT GGATGAAAGA CAATGACATC 
ACAGACATGC TGGACCTTAC CTTCACTGTG AATGAGGAAG TCTTCGGACA GGTAACAGAG 
AGAGAGCTGA AGTCTGGAGG GGCCAACATT AATGTGACAG AGAAGAATAA GAAGGAGTAC 
ATTGACCGCA TGGTGAAATG GAGGGTGGAG AGAGGAGTGG TCAAGCAGAC AGAGGCACTG 
GTCAGGGGCT TCTATGAGGT GGTGGATTCC CGGCTGGTGT CAGTGTTCGA TGCCAGAGAG 
TTGGAGCTGG TCATCGCTGG GACAGCTGAG ATAGACCTCA ATGACTGGAG GAACAACACA 
GAGTATAGAG GAGGCTACCA TGACAGCCAC ATAGTGATCC GGTGGTTCTG GGGGGCCGTG 
GAGCGCTTCA ACAATGAGCA GAGGCTGCGT CTGCTGCAGT TTGTGACTGG CACCTCTAGT 
GTTCCCTACG AGGGCTTCAC TGCGCTACGG GGCAGTAATG GCCTGCGACG CTTCTGTATC 
GAGAAGTGGG GCAAGATCAC CGCTCTTCCA AGGGCACACA CATGCTTCAA CCGGCTGGAT 
CTCCCTCCAT ACCCCTCCTA CACCATGCTC TATGAGAAAC TGCTGATCGC TGTGGAGGAG 
ACCAGCACTT TTGGCCTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014045929.1
CDS	157..4860
Translation	MASPCRSTQNRRRCKDPIRHSYNPEQFRNVNFQNGVPEDVAAMYRSTSDTDLVTSHSRSTLTVSTSLYAIGQTQDILICWDIKEEVDAGDWIGMYLIEEVSSENFLDYKSRGVNGSHKGQIVWKIEGNSYFVEPETKICFKYYHGVSGALRATTPSLTVKNPSVPVFKPVANEEVPQSTGSRRLISFSLSDFHATGLKKGMFFNPDPYLKIAIHPGRNSIFPALPHHGQEKRSGIVCNTINPHWQRERFNFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKQSMPVQRLLEKHAIGDRVVSYTLGRRVPTEHVSGQLQFRFEITSSIHPDDEDMSLSTEPECAAMQETSNQGEGQTSQAQGQAKDTMSLGPGAPELPMDEEEDAGAPELPIGGDTVTDTPMEGHPAGPLGSEQPMDGDGDGDSGSSCSRAKALKTEPSQPEVNGAQGKEEVAVAVAVAAEKLPTVEDNREEGGQQTPSSPQLADPEQGVDSTPAEGQVIPAVLEDMESGAPGEPAEQVVGQEEGDEECCSLRIRTTPRRKNRPCSLPVSELETVIASACGEPETPRSHYIRIHHLLHSLPSTQPGTQEDGEGDGAEAETEPDPDPGPDQLSPRVQDGSEEDEKAEPLTPGPRRTLPRSLSIERLSDLALLLDSEGRHPQGQVRGHQRVSDEDDMEPCCSDSCYEGRGGRHNQAPLVAAARMSVESSDFPSQEDEDMEVNGAESREPGDGMPQWQVTPSTQVQSPQSFIKNGESPVAGPSGKIGRDCPLPSSHPPVSQLPALRHDPHHYPTIDEPLPPNWEARVDSHGRVFYVDHINRTTTWQRPTSAATPDGMRRSGSVQQMEQLNRRYQSIQRTMATDRSENEGSRSRSERTSLNNNSSETDSPSMANELRRDSSSSSSSSSSSPVNSQKITAMLQSPAVKFITHPEFFTVLHANYGAYRMFTSSTCLKHMILKIRRDARNLERYQHNKDLVTFLNRFADAQLELPRGWEIKTDPQGKNFFVDHNSRATTFIDPRIPLQNGCLPNHLTHRQHLQRLRSYSAGEASEVSRSRGAALLSRPGNSVVAAMRSQHHPDPVPQAYNEKIVAFLRQPGILEVLTERQPALSRNHSLREKIHYIRTEGTERLEKLACDADLVILLSLFEEEIMSYVPPSFHPGFSFSPRCSPGSSPQNSPGMSRARAPAPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIVRRDHLLEGTFNQVMAYSRKELQRNKLYITFIGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLDWFRFSGRILGLVLIHQYLLDAFFTRPFYKALLRLATDLSDLEYLDEEFHQSLQWMKDNDITDMLDLTFTVNEEVFGQVTERELKSGGANINVTEKNKKEYIDRMVKWRVERGVVKQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDSHIVIRWFWGAVERFNNEQRLRLLQFVTGTSSVPYEGFTALRGSNGLRRFCIEKWGKITALPRAHTCFNRLDLPPYPSYTMLYEKLLIAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_014190454.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar E3 ubiquitin-protein ligase HECW1-like (LOC106599291), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014190454.1

ORF Insert Sequence:

ATGGCCTCCC CATGTCGTTC AACCCAGAAC CGTCGGCGCT GTAAGGACCC CATCCGCCAC 
AGCTACAACC CCGAACAATT TCGCAACGTT AATTTCCAGA ATGGGGTGCC GGAGGACGTG 
GCAGCCATGT ACCGCTCCAC CAGCGACACG GACCTGGTGA CATCACACAG CCGCTCCACG 
CTTACCGTCA GCACCTCGCT CTACGCCATC GGACAGACCC AGGACATCCT CATCTGCTGG 
GACATCAAGG AGGAGGTGGA CGCTGGGGAC TGGATCGGCA TGTACCTCAT TGAAGAGGTG 
TCGTCGGAGA ACTTTCTGGA CTACAAGAGC CGCGGAGTGA ATGGTTCCCA CAAGGGCCAG 
ATAGTGTGGA AGATTGAGGG CAACTCCTAT TTTGTGGAGC CGGAGACTAA GATCTGTTTT 
AAATATTACC ATGGTGTGAG CGGGGCCCTG CGAGCCACTA CCCCCAGTTT GACTGTGAAG 
AACCCCTCAG TTCCGGTATT TAAGCCCGTC GCCAATGAAG AAGTCCCCCA GAGCACAGGC 
AGTAGAAGAC TCATCAGCTT CTCCCTCTCA GATTTCCATG CAACAGGGCT GAAGAAGGGG 
ATGTTCTTCA ACCCTGACCC CTACCTTAAG ATCGCCATCC ACCCCGGCAG GAACAGCATC 
TTCCCGGCAC TGCCACACCA CGGCCAGGAG AAACGCTCAG GCATCGTCTG TAACACCATC 
AACCCCCACT GGCAGAGAGA GCGCTTCAAC TTTGTGTCTC TGCCCACGGA CGTTCTGGAG 
ATCGAGGTAA AAGACAAGTT TGCCAAGAGT CGTCCTATCA TCAAACGTTT CCTGGGCAAG 
CAGTCCATGC CGGTGCAGAG GCTACTGGAG AAGCATGCTA TTGGTGACCG TGTGGTCAGC 
TACACGCTGG GCCGAAGGGT GCCGACGGAA CATGTCAGTG GACAGCTGCA GTTCAGATTT 
GAGATCACTT CCTCTATTCA TCCAGATGAT GAAGACATGT CTCTCAGCAC AGAGCCTGAG 
TGTGCCGCTA TGCAGGAGAC CAGTAACCAG GGGGAGGGCC AGACCTCCCA GGCCCAGGGC 
CAGGCCAAGG ACACCATGAG CCTGGGTCCG GGGGCTCCCG AACTCCCCAT GGATGAGGAG 
GAAGACGCTG GAGCTCCAGA ACTTCCAATT GGAGGAGATA CAGTTACAGA CACGCCCATG 
GAGGGGCATC CAGCAGGGCC TTTGGGTTCA GAGCAGCCCA TGGATGGAGA TGGAGATGGA 
GACTCAGGGT CTAGCTGTTC CAGAGCCAAG GCCCTAAAAA CAGAACCAAG CCAACCAGAG 
GTCAATGGAG CTCAGGGGAA GGAGGAGGTT GCTGTGGCTG TAGCTGTGGC TGCAGAGAAG 
CTGCCCACGG TAGAGGATAA CAGGGAGGAA GGGGGGCAGC AAACCCCCTC CAGTCCTCAG 
CTTGCTGACC CAGAGCAGGG GGTGGACAGT ACCCCAGCAG AGGGTCAGGT CATCCCTGCT 
GTATTGGAAG ACATGGAGAG TGGAGCGCCT GGGGAGCCAG CAGAACAGGT GGTGGGTCAG 
GAGGAAGGGG ACGAGGAATG CTGCTCCCTG CGCATCAGGA CCACCCCTAG GCGTAAGAAC 
CGGCCCTGCT CCCTGCCCGT GTCAGAGCTG GAGACAGTCA TCGCCTCGGC CTGTGGGGAG 
CCCGAGACCC CTCGCTCCCA CTACATCCGC ATCCACCACC TCCTCCACAG TCTGCCCTCC 
ACCCAGCCAG GCACCCAGGA GGACGGGGAG GGAGATGGGG CTGAGGCTGA AACAGAGCCC 
GATCCAGACC CAGGCCCTGA CCAGCTCAGC CCCAGGGTCC AGGATGGCTC AGAAGAGGAT 
GAAAAGGCAG AACCCCTGAC CCCCGGGCCC AGGAGGACCC TGCCTCGCAG CCTGTCCATC 
GAGCGTCTGT CCGACCTGGC CCTGCTACTG GACAGCGAGG GCCGCCACCC CCAGGGCCAG 
GTCAGGGGTC ACCAGAGAGT GTCCGACGAG GATGACATGG AGCCCTGCTG CAGTGATTCC 
TGCTATGAGG GCCGAGGAGG CCGTCACAAC CAGGCACCTC TAGTGGCTGC AGCCAGGATG 
TCTGTGGAGA GCAGTGATTT CCCCTCCCAG GAGGATGAGG ACATGGAGGT GAACGGAGCG 
GAGAGCAGGG AGCCAGGAGA TGGGATGCCC CAGTGGCAGG TGACCCCGTC CACGCAGGTC 
CAGAGCCCCC AGAGCTTCAT AAAAAACGGA GAGTCACCCG TGGCCGGGCC CAGTGGGAAG 
ATAGGACGAG ATTGTCCCCT ACCGTCCAGT CATCCTCCAG TCAGCCAGCT TCCTGCTCTG 
CGTCATGATC CTCATCATTA CCCCACTATA GATGAACCAC TTCCACCAAA CTGGGAGGCG 
CGTGTGGACA GCCACGGTCG TGTGTTCTAC GTGGACCACA TCAACAGGAC CACCACGTGG 
CAGAGACCCA CCTCAGCAGC CACGCCCGAC GGCATGAGGA GGTCCGGATC CGTTCAACAG 
ATGGAACAGC TCAATAGAAG GTACCAGAGC ATCCAGAGGA CCATGGCCAC AGACAGGAGT 
GAGAATGAAG GAAGCAGAAG CAGGAGTGAG AGAACAAGCC TCAACAACAA CAGCAGTGAG 
ACAGACTCTC CTTCGATGGC TAATGAGCTG AGAAGAGACA GCTCCTCTTC CTCCTCCTCC 
TCGTCATCAT CTCCAGTCAA CAGTCAGAAG ATCACAGCTA TGTTACAGAG TCCAGCGGTG 
AAGTTCATCA CACACCCAGA GTTTTTCACT GTCCTCCATG CCAACTATGG GGCCTACCGC 
ATGTTCACCA GCAGCACGTG CCTGAAGCAC ATGATCCTGA AGATTCGTAG AGACGCTCGT 
AACCTTGAGA GGTACCAGCA CAACAAAGAC CTGGTTACCT TCCTCAACCG CTTCGCTGAC 
GCCCAGCTGG AACTGCCTCG GGGCTGGGAG ATCAAGACTG ACCCTCAGGG CAAGAACTTC 
TTTGTGGACC ACAACAGCCG AGCAACCACG TTCATCGACC CGAGAATCCC TCTTCAGAAC 
GGTTGTCTGC CCAACCACCT GACCCACAGG CAGCACCTGC AGCGATTACG CAGCTACAGT 
GCTGGAGAGG CCTCGGAGGT GTCCCGTAGT CGAGGTGCGG CCTTGTTGTC ACGGCCTGGG 
AACAGTGTGG TGGCCGCGAT GCGGAGCCAG CATCATCCTG ATCCAGTGCC ACAAGCGTAT 
AATGAGAAGA TAGTGGCCTT CCTCCGTCAG CCTGGAATAC TAGAAGTTCT AACGGAGCGC 
CAGCCAGCCC TGTCCAGGAA CCATTCCCTG AGGGAAAAGA TCCACTACAT CAGAACAGAG 
GGCACCGAAA GACTGGAGAA ACTAGCATGT GATGCAGACC TGGTGATACT GTTGAGCTTG 
TTTGAAGAGG AGATCATGTC CTATGTTCCG CCCTCCTTCC ATCCTGGCTT CAGCTTCTCT 
CCGCGTTGTT CACCAGGGTC TTCACCACAG AACTCTCCAG GAATGTCGAG AGCGAGAGCA 
CCTGCCCCGT ACCGGAGAGA CTTTGAGGCC AAACTACGCA ACTTCTACAG GAAACTGGAG 
GCTAAAGGCT ATGGCCAGGG CCCAGGAAAG ATCAAGCTTA TCGTCCGGAG AGACCACCTG 
CTGGAGGGTA CCTTTAACCA GGTCATGGCC TACTCACGCA AGGAACTACA GAGGAATAAG 
CTCTACATCA CTTTCATAGG AGAGGAGGGG CTGGACTACA GTGGGCCATC CAGAGAGTTC 
TTCTTCCTGC TGTCTCAGGA GCTGTTCAAC CCCTACTATG GCCTGTTTGA GTACTCAGCC 
AACGACACAT ACACCGTCCA GATCAGCCCC ATGTCCGCCT TCGTAGAGAA CCATCTGGAT 
TGGTTCCGGT TTAGTGGGCG TATTTTGGGA CTGGTGTTGA TCCACCAGTA TCTCCTGGAT 
GCCTTCTTCA CGCGGCCCTT CTACAAGGCT CTCCTTAGAC TAGCCACTGA CCTGAGTGAT 
CTGGAATACC TAGACGAGGA GTTCCATCAG AGTCTACAGT GGATGAAAGA CAATGACATC 
ACAGACATGC TGGACCTTAC CTTCACTGTG AATGAGGAAG TCTTCGGACA GGTAACAGAG 
AGAGAGCTGA AGTCTGGAGG GGCCAACATT AATGTGACAG AGAAGAATAA GAAGGAGTAC 
ATTGACCGCA TGGTGAAATG GAGGGTGGAG AGAGGAGTGG TCAAGCAGAC AGAGGCACTG 
GTCAGGGGCT TCTATGAGGT GGTGGATTCC CGGCTGGTGT CAGTGTTCGA TGCCAGAGAG 
TTGGAGCTGG TCATCGCTGG GACAGCTGAG ATAGACCTCA ATGACTGGAG GAACAACACA 
GAGTATAGAG GAGGCTACCA TGACAGCCAC ATAGTGATCC GGTGGTTCTG GGGGGCCGTG 
GAGCGCTTCA ACAATGAGCA GAGGCTGCGT CTGCTGCAGT TTGTGACTGG CACCTCTAGT 
GTTCCCTACG AGGGCTTCAC TGCGCTACGG GGCAGTAATG GCCTGCGACG CTTCTGTATC 
GAGAAGTGGG GCAAGATCAC CGCTCTTCCA AGGGCACACA CATGCTTCAA CCGGCTGGAT 
CTCCCTCCAT ACCCCTCCTA CACCATGCTC TATGAGAAAC TGCTGATCGC TGTGGAGGAG 
ACCAGCACTT TTGGCCTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC106599291 cDNA ORF clone, Salmo salar(Atlantic salmon)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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