Tcaf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tcaf1
Entrez Gene ID	362353
Full Name	TRPM8 channel-associated factor 1
Synonyms	Fam115a,RGD1565474
General protein information	Preferred Names TRPM8 channel-associated factor 1 Names TRPM8 channel-associated factor 1 family with sequence similarity 115, member A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q24

mRNA and Protein(s)

mRNA	Protein	Name
XM_342675.7	XP_342676.4	TRPM8 channel-associated factor 1
XM_001072497.5	XP_001072497.2	TRPM8 channel-associated factor 1
XM_003753880.4	XP_003753928.1	TRPM8 channel-associated factor 1
XM_003749739.4	XP_003749787.1	TRPM8 channel-associated factor 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Fam115a	XP_003749787.1
Homo sapiens (human)	FAM115A	NP_055534.1
Pan troglodytes (chimpanzee)	FAM115A	XP_003318910.1
Canis lupus familiaris (dog)	LOC482730	XP_005629583.1
Mus musculus (house mouse)	Fam115a	NP_084206.1
Bos taurus (cattle)	FAM115A	NP_001092524.1

Gene Ontology

The following Tcaf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tcaf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa16174	XM_342675.7 Latest version!	Rattus norvegicus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa16174	XM_003749739.4 Latest version!	Rattus norvegicus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa16174	XM_001072497.5 Latest version!	Rattus norvegicus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa16174	XM_003753880.4 Latest version!	Rattus norvegicus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tcaf1 ( XM_342675.7 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_342676.4 Rattus norvegicus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa16174
Clone ID Related Accession (Same CDS sequence)	XM_342675.7 , XM_001072497.5 , XM_003753880.4 , XM_003749739.4
Accession Version	XM_342675.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	TRPM8 channel-associated factor 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_342675.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTC CATCTGCTAC CTTTGAGGCG CTTATGAATG GAGTAACAAG TTGGGATATC 
CCTGATGATT CCGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCTTT TCCTATAATG 
GTGAATGATG TAGGCCAGGT CCTCATTGCT GCCTCTTCCT ATGGCAGAGG TCGCATGGTG 
GTAGCATCTC ATGAGGACTT CTTGGTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT 
GTTGGATGGC TTCGTTCCTC TTCTGAGTCA ACCATTGGTG TGCACTCATC TTTAGCACCA 
TTGGTGAAAA TCCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAACCAGA AGTGAATGAC 
TCTCTGGGGG TTTACTGCAT CGATGCCTAC AATGAAACCA TGACGGATAA GCTACTCCAG 
TTTGTGAAAC GTGGCGGGGG CTTGCTCATA GGAGGCCAAG CCTGGGATTG GGATAACCTG 
GGTGAAGATG ATAGAGTACT GTTCTCCTTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT 
GTGTACTTCA CTGACAACAA AGCGGACACG AATTGCTATA AAGTCTCCAA GAAGATGCCT 
AAGATACCCA TCTTAGTTCG TTGTGAAGAT GACCTCTCAG AAGACAGAGA TGAGCTTCTA 
CATGGCATCT CAGACCTGGA TATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTG 
CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCCACC ATGGCTGTGT GATAGCTGCT 
GCCCGCTATG GCCGGGGACG GGTGGTTGTA ACTGGTCATA AGGTATTATT CACTGTGGGT 
AAACTCGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTAG ATGGCGGCCG CAGAGGCAAG 
ATTGTGGTAC AGACCGAGCT GAGAACACTA AGTGGTCTGC TTGCAGTAGG GGGTATAGAC 
ACCAGCATTG AGCCCCAACT GACCAGTGAC GCAAGTGTCT ATTGCTTCGA ACCCACAAGT 
GATGTGGGAG TTAAGGAACT ACAGGAGTTT GTAGCTGAGG GTGGTGGGCT GTTTGTTGGA 
ACCCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCACTGGC TCGATTCCCA 
GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAAAGCCT CAATCCAGGA 
CCTTTCCATA TCCCAAAAGC AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTGGCAGAG 
TTCCAGGTGA TAATGGGCAG GAAAAGAGGA AATGTGGAGA AGGGCTGGCT AGCCAAGCTG 
GGCCCAGATG GGGCAGCTTT CCTGCAGATT CCTGCAGAGG AGATCCCTGC TTACATGTCC 
GTGCACAGAC TCCTGCGGAA GCTGCTGAGT CGCTACCGGC TTCCAGTGGC AACCAGAGAG 
AACCCGGTCA TCAATGACTG TTGTAGGGGG GCCATGCTTT CCCTGGCCAC AGGTCTGGCC 
CACTCTGGAA GTGACCTCTC ACTGCTAATC CCAGAAATTG AAGATGTGTA CAGCAGCTGC 
TACCTACGCC CCTCAGAGTC TCCCATCACC GTGGATGTCA ACTGCACCAA TCCAGGCACC 
CGATACTGCT GGATGAGCAC TGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTTTCC 
TTGCCTGAAG ATGCTGCCTC TGCTGATCTG AAGATACAAA TTGGCTGTCA CACTGATGAC 
CTGACCAGGG CCAGCAAGCT CTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAT 
AAACCCATGA AATCAATCAA CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG 
AATAGCAAAC TGGGTACTGT GCCCCTCACA GTGAAGGGAG CCGTTCGTGC CCCATTCTAC 
AAGCTAGGGG AGACATCCAA GGAAGAGTGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCG 
TGGGGAGAAC TGGCCACTGA CAACCTCATC TTGACTGTGC CTACTGCAAA TCTTCGAACT 
CTGGAGAACC CTGAGCCACT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCTAAG 
TTGGGAGGCG AGCCCTTCCC TTTGCGGCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC 
TCAGTAGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG 
CTCATTAGTG AGAAGCTCAT CCGGACCAAG GGACTGTGGG GTCCTGTGCA TGAGCTGGGC 
CGCAACCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACATGCAAC 
CTGTGGTGTG TTTATGTGCA TGAAACAGTT CTGGGCATAC CTCGAAGCCG TGCCAACATT 
GCGCTATGGC CCCCTGTTCG GGAGAAGAGA GTCCGAATGT ATTTAAGCAA GGGACCCAAT 
GTGAAAAACT GGAATGCCTG GACTGCGTTG GAAACGTACC TACAGCTTCA AGAGGCCTTT 
GGTTGGGAGC CATTTATTCG TCTCTTCACT GAGTACAGAA ACCAGACAAC GAACTCGCCC 
ACAGACAACG TCAACAAAAT GAATCTCTGG GTCAAGATGT TCTCCCAGCA AGTGCAGAAG 
AATCTGGCTC CGTTCTTTGA GGCCTGGGCC TGGCCTATCC AGAAAGAAGT GGCTACAAGC 
TTAGCCTATC TGCCTGAATG GAAAGAAAAT ATAATGAAAT TGTATCTTCT CACACAGTTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_342676.4
CDS	308..3070
Translation	MATPSATFEALMNGVTSWDIPDDSVPCELLLIGEASFPIMVNDVGQVLIAASSYGRGRMVVASHEDFLVESQLFVFLVNAVGWLRSSSESTIGVHSSLAPLVKILESCGIESKIEPEVNDSLGVYCIDAYNETMTDKLLQFVKRGGGLLIGGQAWDWDNLGEDDRVLFSFPGNLVTSVAGVYFTDNKADTNCYKVSKKMPKIPILVRCEDDLSEDRDELLHGISDLDITNSDCFPSQLLVHGSLAFPLGLDTHHGCVIAAARYGRGRVVVTGHKVLFTVGKLGPFLLNAVRWLDGGRRGKIVVQTELRTLSGLLAVGGIDTSIEPQLTSDASVYCFEPTSDVGVKELQEFVAEGGGLFVGTQAWWWAFKNPGVSPLARFPGNLLLNAFGISITSQSLNPGPFHIPKAGTRTYHFRSTLAEFQVIMGRKRGNVEKGWLAKLGPDGAAFLQIPAEEIPAYMSVHRLLRKLLSRYRLPVATRENPVINDCCRGAMLSLATGLAHSGSDLSLLIPEIEDVYSSCYLRPSESPITVDVNCTNPGTRYCWMSTGLYIPGRQVIDVSLPEDAASADLKIQIGCHTDDLTRASKLFRGPLVINRCCLDKPMKSINCLWGGLLYIIVPQNSKLGTVPLTVKGAVRAPFYKLGETSKEEWKRRLLEYPGPWGELATDNLILTVPTANLRTLENPEPLLRLWDEVMQAVAKLGGEPFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQELISEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCNLWCVYVHETVLGIPRSRANIALWPPVREKRVRMYLSKGPNVKNWNAWTALETYLQLQEAFGWEPFIRLFTEYRNQTTNSPTDNVNKMNLWVKMFSQQVQKNLAPFFEAWAWPIQKEVATSLAYLPEWKENIMKLYLLTQL

Target ORF information:

RefSeq Version	XM_342675.7
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_342675.7

ORF Insert Sequence:

ATGGCAACTC CATCTGCTAC CTTTGAGGCG CTTATGAATG GAGTAACAAG TTGGGATATC 
CCTGATGATT CCGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCTTT TCCTATAATG 
GTGAATGATG TAGGCCAGGT CCTCATTGCT GCCTCTTCCT ATGGCAGAGG TCGCATGGTG 
GTAGCATCTC ATGAGGACTT CTTGGTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT 
GTTGGATGGC TTCGTTCCTC TTCTGAGTCA ACCATTGGTG TGCACTCATC TTTAGCACCA 
TTGGTGAAAA TCCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAACCAGA AGTGAATGAC 
TCTCTGGGGG TTTACTGCAT CGATGCCTAC AATGAAACCA TGACGGATAA GCTACTCCAG 
TTTGTGAAAC GTGGCGGGGG CTTGCTCATA GGAGGCCAAG CCTGGGATTG GGATAACCTG 
GGTGAAGATG ATAGAGTACT GTTCTCCTTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT 
GTGTACTTCA CTGACAACAA AGCGGACACG AATTGCTATA AAGTCTCCAA GAAGATGCCT 
AAGATACCCA TCTTAGTTCG TTGTGAAGAT GACCTCTCAG AAGACAGAGA TGAGCTTCTA 
CATGGCATCT CAGACCTGGA TATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTG 
CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCCACC ATGGCTGTGT GATAGCTGCT 
GCCCGCTATG GCCGGGGACG GGTGGTTGTA ACTGGTCATA AGGTATTATT CACTGTGGGT 
AAACTCGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTAG ATGGCGGCCG CAGAGGCAAG 
ATTGTGGTAC AGACCGAGCT GAGAACACTA AGTGGTCTGC TTGCAGTAGG GGGTATAGAC 
ACCAGCATTG AGCCCCAACT GACCAGTGAC GCAAGTGTCT ATTGCTTCGA ACCCACAAGT 
GATGTGGGAG TTAAGGAACT ACAGGAGTTT GTAGCTGAGG GTGGTGGGCT GTTTGTTGGA 
ACCCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCACTGGC TCGATTCCCA 
GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAAAGCCT CAATCCAGGA 
CCTTTCCATA TCCCAAAAGC AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTGGCAGAG 
TTCCAGGTGA TAATGGGCAG GAAAAGAGGA AATGTGGAGA AGGGCTGGCT AGCCAAGCTG 
GGCCCAGATG GGGCAGCTTT CCTGCAGATT CCTGCAGAGG AGATCCCTGC TTACATGTCC 
GTGCACAGAC TCCTGCGGAA GCTGCTGAGT CGCTACCGGC TTCCAGTGGC AACCAGAGAG 
AACCCGGTCA TCAATGACTG TTGTAGGGGG GCCATGCTTT CCCTGGCCAC AGGTCTGGCC 
CACTCTGGAA GTGACCTCTC ACTGCTAATC CCAGAAATTG AAGATGTGTA CAGCAGCTGC 
TACCTACGCC CCTCAGAGTC TCCCATCACC GTGGATGTCA ACTGCACCAA TCCAGGCACC 
CGATACTGCT GGATGAGCAC TGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTTTCC 
TTGCCTGAAG ATGCTGCCTC TGCTGATCTG AAGATACAAA TTGGCTGTCA CACTGATGAC 
CTGACCAGGG CCAGCAAGCT CTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAT 
AAACCCATGA AATCAATCAA CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG 
AATAGCAAAC TGGGTACTGT GCCCCTCACA GTGAAGGGAG CCGTTCGTGC CCCATTCTAC 
AAGCTAGGGG AGACATCCAA GGAAGAGTGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCG 
TGGGGAGAAC TGGCCACTGA CAACCTCATC TTGACTGTGC CTACTGCAAA TCTTCGAACT 
CTGGAGAACC CTGAGCCACT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCTAAG 
TTGGGAGGCG AGCCCTTCCC TTTGCGGCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC 
TCAGTAGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG 
CTCATTAGTG AGAAGCTCAT CCGGACCAAG GGACTGTGGG GTCCTGTGCA TGAGCTGGGC 
CGCAACCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACATGCAAC 
CTGTGGTGTG TTTATGTGCA TGAAACAGTT CTGGGCATAC CTCGAAGCCG TGCCAACATT 
GCGCTATGGC CCCCTGTTCG GGAGAAGAGA GTCCGAATGT ATTTAAGCAA GGGACCCAAT 
GTGAAAAACT GGAATGCCTG GACTGCGTTG GAAACGTACC TACAGCTTCA AGAGGCCTTT 
GGTTGGGAGC CATTTATTCG TCTCTTCACT GAGTACAGAA ACCAGACAAC GAACTCGCCC 
ACAGACAACG TCAACAAAAT GAATCTCTGG GTCAAGATGT TCTCCCAGCA AGTGCAGAAG 
AATCTGGCTC CGTTCTTTGA GGCCTGGGCC TGGCCTATCC AGAAAGAAGT GGCTACAAGC 
TTAGCCTATC TGCCTGAATG GAAAGAAAAT ATAATGAAAT TGTATCTTCT CACACAGTTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tcaf1 ( XM_001072497.5 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_001072497.2 Rattus norvegicus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa16174
Clone ID Related Accession (Same CDS sequence)	XM_342675.7 , XM_001072497.5 , XM_003753880.4 , XM_003749739.4
Accession Version	XM_001072497.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	TRPM8 channel-associated factor 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000072.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_001072497.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTC CATCTGCTAC CTTTGAGGCG CTTATGAATG GAGTAACAAG TTGGGATATC 
CCTGATGATT CCGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCTTT TCCTATAATG 
GTGAATGATG TAGGCCAGGT CCTCATTGCT GCCTCTTCCT ATGGCAGAGG TCGCATGGTG 
GTAGCATCTC ATGAGGACTT CTTGGTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT 
GTTGGATGGC TTCGTTCCTC TTCTGAGTCA ACCATTGGTG TGCACTCATC TTTAGCACCA 
TTGGTGAAAA TCCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAACCAGA AGTGAATGAC 
TCTCTGGGGG TTTACTGCAT CGATGCCTAC AATGAAACCA TGACGGATAA GCTACTCCAG 
TTTGTGAAAC GTGGCGGGGG CTTGCTCATA GGAGGCCAAG CCTGGGATTG GGATAACCTG 
GGTGAAGATG ATAGAGTACT GTTCTCCTTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT 
GTGTACTTCA CTGACAACAA AGCGGACACG AATTGCTATA AAGTCTCCAA GAAGATGCCT 
AAGATACCCA TCTTAGTTCG TTGTGAAGAT GACCTCTCAG AAGACAGAGA TGAGCTTCTA 
CATGGCATCT CAGACCTGGA TATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTG 
CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCCACC ATGGCTGTGT GATAGCTGCT 
GCCCGCTATG GCCGGGGACG GGTGGTTGTA ACTGGTCATA AGGTATTATT CACTGTGGGT 
AAACTCGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTAG ATGGCGGCCG CAGAGGCAAG 
ATTGTGGTAC AGACCGAGCT GAGAACACTA AGTGGTCTGC TTGCAGTAGG GGGTATAGAC 
ACCAGCATTG AGCCCCAACT GACCAGTGAC GCAAGTGTCT ATTGCTTCGA ACCCACAAGT 
GATGTGGGAG TTAAGGAACT ACAGGAGTTT GTAGCTGAGG GTGGTGGGCT GTTTGTTGGA 
ACCCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCACTGGC TCGATTCCCA 
GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAAAGCCT CAATCCAGGA 
CCTTTCCATA TCCCAAAAGC AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTGGCAGAG 
TTCCAGGTGA TAATGGGCAG GAAAAGAGGA AATGTGGAGA AGGGCTGGCT AGCCAAGCTG 
GGCCCAGATG GGGCAGCTTT CCTGCAGATT CCTGCAGAGG AGATCCCTGC TTACATGTCC 
GTGCACAGAC TCCTGCGGAA GCTGCTGAGT CGCTACCGGC TTCCAGTGGC AACCAGAGAG 
AACCCGGTCA TCAATGACTG TTGTAGGGGG GCCATGCTTT CCCTGGCCAC AGGTCTGGCC 
CACTCTGGAA GTGACCTCTC ACTGCTAATC CCAGAAATTG AAGATGTGTA CAGCAGCTGC 
TACCTACGCC CCTCAGAGTC TCCCATCACC GTGGATGTCA ACTGCACCAA TCCAGGCACC 
CGATACTGCT GGATGAGCAC TGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTTTCC 
TTGCCTGAAG ATGCTGCCTC TGCTGATCTG AAGATACAAA TTGGCTGTCA CACTGATGAC 
CTGACCAGGG CCAGCAAGCT CTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAT 
AAACCCATGA AATCAATCAA CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG 
AATAGCAAAC TGGGTACTGT GCCCCTCACA GTGAAGGGAG CCGTTCGTGC CCCATTCTAC 
AAGCTAGGGG AGACATCCAA GGAAGAGTGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCG 
TGGGGAGAAC TGGCCACTGA CAACCTCATC TTGACTGTGC CTACTGCAAA TCTTCGAACT 
CTGGAGAACC CTGAGCCACT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCTAAG 
TTGGGAGGCG AGCCCTTCCC TTTGCGGCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC 
TCAGTAGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG 
CTCATTAGTG AGAAGCTCAT CCGGACCAAG GGACTGTGGG GTCCTGTGCA TGAGCTGGGC 
CGCAACCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACATGCAAC 
CTGTGGTGTG TTTATGTGCA TGAAACAGTT CTGGGCATAC CTCGAAGCCG TGCCAACATT 
GCGCTATGGC CCCCTGTTCG GGAGAAGAGA GTCCGAATGT ATTTAAGCAA GGGACCCAAT 
GTGAAAAACT GGAATGCCTG GACTGCGTTG GAAACGTACC TACAGCTTCA AGAGGCCTTT 
GGTTGGGAGC CATTTATTCG TCTCTTCACT GAGTACAGAA ACCAGACAAC GAACTCGCCC 
ACAGACAACG TCAACAAAAT GAATCTCTGG GTCAAGATGT TCTCCCAGCA AGTGCAGAAG 
AATCTGGCTC CGTTCTTTGA GGCCTGGGCC TGGCCTATCC AGAAAGAAGT GGCTACAAGC 
TTAGCCTATC TGCCTGAATG GAAAGAAAAT ATAATGAAAT TGTATCTTCT CACACAGTTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001072497.2
CDS	308..3070
Translation	MATPSATFEALMNGVTSWDIPDDSVPCELLLIGEASFPIMVNDVGQVLIAASSYGRGRMVVASHEDFLVESQLFVFLVNAVGWLRSSSESTIGVHSSLAPLVKILESCGIESKIEPEVNDSLGVYCIDAYNETMTDKLLQFVKRGGGLLIGGQAWDWDNLGEDDRVLFSFPGNLVTSVAGVYFTDNKADTNCYKVSKKMPKIPILVRCEDDLSEDRDELLHGISDLDITNSDCFPSQLLVHGSLAFPLGLDTHHGCVIAAARYGRGRVVVTGHKVLFTVGKLGPFLLNAVRWLDGGRRGKIVVQTELRTLSGLLAVGGIDTSIEPQLTSDASVYCFEPTSDVGVKELQEFVAEGGGLFVGTQAWWWAFKNPGVSPLARFPGNLLLNAFGISITSQSLNPGPFHIPKAGTRTYHFRSTLAEFQVIMGRKRGNVEKGWLAKLGPDGAAFLQIPAEEIPAYMSVHRLLRKLLSRYRLPVATRENPVINDCCRGAMLSLATGLAHSGSDLSLLIPEIEDVYSSCYLRPSESPITVDVNCTNPGTRYCWMSTGLYIPGRQVIDVSLPEDAASADLKIQIGCHTDDLTRASKLFRGPLVINRCCLDKPMKSINCLWGGLLYIIVPQNSKLGTVPLTVKGAVRAPFYKLGETSKEEWKRRLLEYPGPWGELATDNLILTVPTANLRTLENPEPLLRLWDEVMQAVAKLGGEPFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQELISEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCNLWCVYVHETVLGIPRSRANIALWPPVREKRVRMYLSKGPNVKNWNAWTALETYLQLQEAFGWEPFIRLFTEYRNQTTNSPTDNVNKMNLWVKMFSQQVQKNLAPFFEAWAWPIQKEVATSLAYLPEWKENIMKLYLLTQL

Target ORF information:

RefSeq Version	XM_001072497.5
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001072497.5

ORF Insert Sequence:

ATGGCAACTC CATCTGCTAC CTTTGAGGCG CTTATGAATG GAGTAACAAG TTGGGATATC 
CCTGATGATT CCGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCTTT TCCTATAATG 
GTGAATGATG TAGGCCAGGT CCTCATTGCT GCCTCTTCCT ATGGCAGAGG TCGCATGGTG 
GTAGCATCTC ATGAGGACTT CTTGGTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT 
GTTGGATGGC TTCGTTCCTC TTCTGAGTCA ACCATTGGTG TGCACTCATC TTTAGCACCA 
TTGGTGAAAA TCCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAACCAGA AGTGAATGAC 
TCTCTGGGGG TTTACTGCAT CGATGCCTAC AATGAAACCA TGACGGATAA GCTACTCCAG 
TTTGTGAAAC GTGGCGGGGG CTTGCTCATA GGAGGCCAAG CCTGGGATTG GGATAACCTG 
GGTGAAGATG ATAGAGTACT GTTCTCCTTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT 
GTGTACTTCA CTGACAACAA AGCGGACACG AATTGCTATA AAGTCTCCAA GAAGATGCCT 
AAGATACCCA TCTTAGTTCG TTGTGAAGAT GACCTCTCAG AAGACAGAGA TGAGCTTCTA 
CATGGCATCT CAGACCTGGA TATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTG 
CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCCACC ATGGCTGTGT GATAGCTGCT 
GCCCGCTATG GCCGGGGACG GGTGGTTGTA ACTGGTCATA AGGTATTATT CACTGTGGGT 
AAACTCGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTAG ATGGCGGCCG CAGAGGCAAG 
ATTGTGGTAC AGACCGAGCT GAGAACACTA AGTGGTCTGC TTGCAGTAGG GGGTATAGAC 
ACCAGCATTG AGCCCCAACT GACCAGTGAC GCAAGTGTCT ATTGCTTCGA ACCCACAAGT 
GATGTGGGAG TTAAGGAACT ACAGGAGTTT GTAGCTGAGG GTGGTGGGCT GTTTGTTGGA 
ACCCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCACTGGC TCGATTCCCA 
GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAAAGCCT CAATCCAGGA 
CCTTTCCATA TCCCAAAAGC AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTGGCAGAG 
TTCCAGGTGA TAATGGGCAG GAAAAGAGGA AATGTGGAGA AGGGCTGGCT AGCCAAGCTG 
GGCCCAGATG GGGCAGCTTT CCTGCAGATT CCTGCAGAGG AGATCCCTGC TTACATGTCC 
GTGCACAGAC TCCTGCGGAA GCTGCTGAGT CGCTACCGGC TTCCAGTGGC AACCAGAGAG 
AACCCGGTCA TCAATGACTG TTGTAGGGGG GCCATGCTTT CCCTGGCCAC AGGTCTGGCC 
CACTCTGGAA GTGACCTCTC ACTGCTAATC CCAGAAATTG AAGATGTGTA CAGCAGCTGC 
TACCTACGCC CCTCAGAGTC TCCCATCACC GTGGATGTCA ACTGCACCAA TCCAGGCACC 
CGATACTGCT GGATGAGCAC TGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTTTCC 
TTGCCTGAAG ATGCTGCCTC TGCTGATCTG AAGATACAAA TTGGCTGTCA CACTGATGAC 
CTGACCAGGG CCAGCAAGCT CTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAT 
AAACCCATGA AATCAATCAA CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG 
AATAGCAAAC TGGGTACTGT GCCCCTCACA GTGAAGGGAG CCGTTCGTGC CCCATTCTAC 
AAGCTAGGGG AGACATCCAA GGAAGAGTGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCG 
TGGGGAGAAC TGGCCACTGA CAACCTCATC TTGACTGTGC CTACTGCAAA TCTTCGAACT 
CTGGAGAACC CTGAGCCACT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCTAAG 
TTGGGAGGCG AGCCCTTCCC TTTGCGGCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC 
TCAGTAGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG 
CTCATTAGTG AGAAGCTCAT CCGGACCAAG GGACTGTGGG GTCCTGTGCA TGAGCTGGGC 
CGCAACCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACATGCAAC 
CTGTGGTGTG TTTATGTGCA TGAAACAGTT CTGGGCATAC CTCGAAGCCG TGCCAACATT 
GCGCTATGGC CCCCTGTTCG GGAGAAGAGA GTCCGAATGT ATTTAAGCAA GGGACCCAAT 
GTGAAAAACT GGAATGCCTG GACTGCGTTG GAAACGTACC TACAGCTTCA AGAGGCCTTT 
GGTTGGGAGC CATTTATTCG TCTCTTCACT GAGTACAGAA ACCAGACAAC GAACTCGCCC 
ACAGACAACG TCAACAAAAT GAATCTCTGG GTCAAGATGT TCTCCCAGCA AGTGCAGAAG 
AATCTGGCTC CGTTCTTTGA GGCCTGGGCC TGGCCTATCC AGAAAGAAGT GGCTACAAGC 
TTAGCCTATC TGCCTGAATG GAAAGAAAAT ATAATGAAAT TGTATCTTCT CACACAGTTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tcaf1 ( XM_003753880.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_003753928.1 Rattus norvegicus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa16174
Clone ID Related Accession (Same CDS sequence)	XM_342675.7 , XM_001072497.5 , XM_003753880.4 , XM_003749739.4
Accession Version	XM_003753880.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	TRPM8 channel-associated factor 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000072.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_003753880.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTC CATCTGCTAC CTTTGAGGCG CTTATGAATG GAGTAACAAG TTGGGATATC 
CCTGATGATT CCGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCTTT TCCTATAATG 
GTGAATGATG TAGGCCAGGT CCTCATTGCT GCCTCTTCCT ATGGCAGAGG TCGCATGGTG 
GTAGCATCTC ATGAGGACTT CTTGGTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT 
GTTGGATGGC TTCGTTCCTC TTCTGAGTCA ACCATTGGTG TGCACTCATC TTTAGCACCA 
TTGGTGAAAA TCCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAACCAGA AGTGAATGAC 
TCTCTGGGGG TTTACTGCAT CGATGCCTAC AATGAAACCA TGACGGATAA GCTACTCCAG 
TTTGTGAAAC GTGGCGGGGG CTTGCTCATA GGAGGCCAAG CCTGGGATTG GGATAACCTG 
GGTGAAGATG ATAGAGTACT GTTCTCCTTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT 
GTGTACTTCA CTGACAACAA AGCGGACACG AATTGCTATA AAGTCTCCAA GAAGATGCCT 
AAGATACCCA TCTTAGTTCG TTGTGAAGAT GACCTCTCAG AAGACAGAGA TGAGCTTCTA 
CATGGCATCT CAGACCTGGA TATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTG 
CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCCACC ATGGCTGTGT GATAGCTGCT 
GCCCGCTATG GCCGGGGACG GGTGGTTGTA ACTGGTCATA AGGTATTATT CACTGTGGGT 
AAACTCGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTAG ATGGCGGCCG CAGAGGCAAG 
ATTGTGGTAC AGACCGAGCT GAGAACACTA AGTGGTCTGC TTGCAGTAGG GGGTATAGAC 
ACCAGCATTG AGCCCCAACT GACCAGTGAC GCAAGTGTCT ATTGCTTCGA ACCCACAAGT 
GATGTGGGAG TTAAGGAACT ACAGGAGTTT GTAGCTGAGG GTGGTGGGCT GTTTGTTGGA 
ACCCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCACTGGC TCGATTCCCA 
GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAAAGCCT CAATCCAGGA 
CCTTTCCATA TCCCAAAAGC AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTGGCAGAG 
TTCCAGGTGA TAATGGGCAG GAAAAGAGGA AATGTGGAGA AGGGCTGGCT AGCCAAGCTG 
GGCCCAGATG GGGCAGCTTT CCTGCAGATT CCTGCAGAGG AGATCCCTGC TTACATGTCC 
GTGCACAGAC TCCTGCGGAA GCTGCTGAGT CGCTACCGGC TTCCAGTGGC AACCAGAGAG 
AACCCGGTCA TCAATGACTG TTGTAGGGGG GCCATGCTTT CCCTGGCCAC AGGTCTGGCC 
CACTCTGGAA GTGACCTCTC ACTGCTAATC CCAGAAATTG AAGATGTGTA CAGCAGCTGC 
TACCTACGCC CCTCAGAGTC TCCCATCACC GTGGATGTCA ACTGCACCAA TCCAGGCACC 
CGATACTGCT GGATGAGCAC TGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTTTCC 
TTGCCTGAAG ATGCTGCCTC TGCTGATCTG AAGATACAAA TTGGCTGTCA CACTGATGAC 
CTGACCAGGG CCAGCAAGCT CTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAT 
AAACCCATGA AATCAATCAA CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG 
AATAGCAAAC TGGGTACTGT GCCCCTCACA GTGAAGGGAG CCGTTCGTGC CCCATTCTAC 
AAGCTAGGGG AGACATCCAA GGAAGAGTGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCG 
TGGGGAGAAC TGGCCACTGA CAACCTCATC TTGACTGTGC CTACTGCAAA TCTTCGAACT 
CTGGAGAACC CTGAGCCACT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCTAAG 
TTGGGAGGCG AGCCCTTCCC TTTGCGGCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC 
TCAGTAGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG 
CTCATTAGTG AGAAGCTCAT CCGGACCAAG GGACTGTGGG GTCCTGTGCA TGAGCTGGGC 
CGCAACCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACATGCAAC 
CTGTGGTGTG TTTATGTGCA TGAAACAGTT CTGGGCATAC CTCGAAGCCG TGCCAACATT 
GCGCTATGGC CCCCTGTTCG GGAGAAGAGA GTCCGAATGT ATTTAAGCAA GGGACCCAAT 
GTGAAAAACT GGAATGCCTG GACTGCGTTG GAAACGTACC TACAGCTTCA AGAGGCCTTT 
GGTTGGGAGC CATTTATTCG TCTCTTCACT GAGTACAGAA ACCAGACAAC GAACTCGCCC 
ACAGACAACG TCAACAAAAT GAATCTCTGG GTCAAGATGT TCTCCCAGCA AGTGCAGAAG 
AATCTGGCTC CGTTCTTTGA GGCCTGGGCC TGGCCTATCC AGAAAGAAGT GGCTACAAGC 
TTAGCCTATC TGCCTGAATG GAAAGAAAAT ATAATGAAAT TGTATCTTCT CACACAGTTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003753928.1
CDS	171..2933
Translation	MATPSATFEALMNGVTSWDIPDDSVPCELLLIGEASFPIMVNDVGQVLIAASSYGRGRMVVASHEDFLVESQLFVFLVNAVGWLRSSSESTIGVHSSLAPLVKILESCGIESKIEPEVNDSLGVYCIDAYNETMTDKLLQFVKRGGGLLIGGQAWDWDNLGEDDRVLFSFPGNLVTSVAGVYFTDNKADTNCYKVSKKMPKIPILVRCEDDLSEDRDELLHGISDLDITNSDCFPSQLLVHGSLAFPLGLDTHHGCVIAAARYGRGRVVVTGHKVLFTVGKLGPFLLNAVRWLDGGRRGKIVVQTELRTLSGLLAVGGIDTSIEPQLTSDASVYCFEPTSDVGVKELQEFVAEGGGLFVGTQAWWWAFKNPGVSPLARFPGNLLLNAFGISITSQSLNPGPFHIPKAGTRTYHFRSTLAEFQVIMGRKRGNVEKGWLAKLGPDGAAFLQIPAEEIPAYMSVHRLLRKLLSRYRLPVATRENPVINDCCRGAMLSLATGLAHSGSDLSLLIPEIEDVYSSCYLRPSESPITVDVNCTNPGTRYCWMSTGLYIPGRQVIDVSLPEDAASADLKIQIGCHTDDLTRASKLFRGPLVINRCCLDKPMKSINCLWGGLLYIIVPQNSKLGTVPLTVKGAVRAPFYKLGETSKEEWKRRLLEYPGPWGELATDNLILTVPTANLRTLENPEPLLRLWDEVMQAVAKLGGEPFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQELISEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCNLWCVYVHETVLGIPRSRANIALWPPVREKRVRMYLSKGPNVKNWNAWTALETYLQLQEAFGWEPFIRLFTEYRNQTTNSPTDNVNKMNLWVKMFSQQVQKNLAPFFEAWAWPIQKEVATSLAYLPEWKENIMKLYLLTQL

Target ORF information:

RefSeq Version	XM_003753880.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003753880.4

ORF Insert Sequence:

ATGGCAACTC CATCTGCTAC CTTTGAGGCG CTTATGAATG GAGTAACAAG TTGGGATATC 
CCTGATGATT CCGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCTTT TCCTATAATG 
GTGAATGATG TAGGCCAGGT CCTCATTGCT GCCTCTTCCT ATGGCAGAGG TCGCATGGTG 
GTAGCATCTC ATGAGGACTT CTTGGTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT 
GTTGGATGGC TTCGTTCCTC TTCTGAGTCA ACCATTGGTG TGCACTCATC TTTAGCACCA 
TTGGTGAAAA TCCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAACCAGA AGTGAATGAC 
TCTCTGGGGG TTTACTGCAT CGATGCCTAC AATGAAACCA TGACGGATAA GCTACTCCAG 
TTTGTGAAAC GTGGCGGGGG CTTGCTCATA GGAGGCCAAG CCTGGGATTG GGATAACCTG 
GGTGAAGATG ATAGAGTACT GTTCTCCTTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT 
GTGTACTTCA CTGACAACAA AGCGGACACG AATTGCTATA AAGTCTCCAA GAAGATGCCT 
AAGATACCCA TCTTAGTTCG TTGTGAAGAT GACCTCTCAG AAGACAGAGA TGAGCTTCTA 
CATGGCATCT CAGACCTGGA TATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTG 
CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCCACC ATGGCTGTGT GATAGCTGCT 
GCCCGCTATG GCCGGGGACG GGTGGTTGTA ACTGGTCATA AGGTATTATT CACTGTGGGT 
AAACTCGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTAG ATGGCGGCCG CAGAGGCAAG 
ATTGTGGTAC AGACCGAGCT GAGAACACTA AGTGGTCTGC TTGCAGTAGG GGGTATAGAC 
ACCAGCATTG AGCCCCAACT GACCAGTGAC GCAAGTGTCT ATTGCTTCGA ACCCACAAGT 
GATGTGGGAG TTAAGGAACT ACAGGAGTTT GTAGCTGAGG GTGGTGGGCT GTTTGTTGGA 
ACCCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCACTGGC TCGATTCCCA 
GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAAAGCCT CAATCCAGGA 
CCTTTCCATA TCCCAAAAGC AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTGGCAGAG 
TTCCAGGTGA TAATGGGCAG GAAAAGAGGA AATGTGGAGA AGGGCTGGCT AGCCAAGCTG 
GGCCCAGATG GGGCAGCTTT CCTGCAGATT CCTGCAGAGG AGATCCCTGC TTACATGTCC 
GTGCACAGAC TCCTGCGGAA GCTGCTGAGT CGCTACCGGC TTCCAGTGGC AACCAGAGAG 
AACCCGGTCA TCAATGACTG TTGTAGGGGG GCCATGCTTT CCCTGGCCAC AGGTCTGGCC 
CACTCTGGAA GTGACCTCTC ACTGCTAATC CCAGAAATTG AAGATGTGTA CAGCAGCTGC 
TACCTACGCC CCTCAGAGTC TCCCATCACC GTGGATGTCA ACTGCACCAA TCCAGGCACC 
CGATACTGCT GGATGAGCAC TGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTTTCC 
TTGCCTGAAG ATGCTGCCTC TGCTGATCTG AAGATACAAA TTGGCTGTCA CACTGATGAC 
CTGACCAGGG CCAGCAAGCT CTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAT 
AAACCCATGA AATCAATCAA CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG 
AATAGCAAAC TGGGTACTGT GCCCCTCACA GTGAAGGGAG CCGTTCGTGC CCCATTCTAC 
AAGCTAGGGG AGACATCCAA GGAAGAGTGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCG 
TGGGGAGAAC TGGCCACTGA CAACCTCATC TTGACTGTGC CTACTGCAAA TCTTCGAACT 
CTGGAGAACC CTGAGCCACT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCTAAG 
TTGGGAGGCG AGCCCTTCCC TTTGCGGCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC 
TCAGTAGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG 
CTCATTAGTG AGAAGCTCAT CCGGACCAAG GGACTGTGGG GTCCTGTGCA TGAGCTGGGC 
CGCAACCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACATGCAAC 
CTGTGGTGTG TTTATGTGCA TGAAACAGTT CTGGGCATAC CTCGAAGCCG TGCCAACATT 
GCGCTATGGC CCCCTGTTCG GGAGAAGAGA GTCCGAATGT ATTTAAGCAA GGGACCCAAT 
GTGAAAAACT GGAATGCCTG GACTGCGTTG GAAACGTACC TACAGCTTCA AGAGGCCTTT 
GGTTGGGAGC CATTTATTCG TCTCTTCACT GAGTACAGAA ACCAGACAAC GAACTCGCCC 
ACAGACAACG TCAACAAAAT GAATCTCTGG GTCAAGATGT TCTCCCAGCA AGTGCAGAAG 
AATCTGGCTC CGTTCTTTGA GGCCTGGGCC TGGCCTATCC AGAAAGAAGT GGCTACAAGC 
TTAGCCTATC TGCCTGAATG GAAAGAAAAT ATAATGAAAT TGTATCTTCT CACACAGTTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tcaf1 ( XM_003749739.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_003749787.1 Rattus norvegicus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa16174
Clone ID Related Accession (Same CDS sequence)	XM_342675.7 , XM_001072497.5 , XM_003753880.4 , XM_003749739.4
Accession Version	XM_003749739.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	TRPM8 channel-associated factor 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_003749739.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTC CATCTGCTAC CTTTGAGGCG CTTATGAATG GAGTAACAAG TTGGGATATC 
CCTGATGATT CCGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCTTT TCCTATAATG 
GTGAATGATG TAGGCCAGGT CCTCATTGCT GCCTCTTCCT ATGGCAGAGG TCGCATGGTG 
GTAGCATCTC ATGAGGACTT CTTGGTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT 
GTTGGATGGC TTCGTTCCTC TTCTGAGTCA ACCATTGGTG TGCACTCATC TTTAGCACCA 
TTGGTGAAAA TCCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAACCAGA AGTGAATGAC 
TCTCTGGGGG TTTACTGCAT CGATGCCTAC AATGAAACCA TGACGGATAA GCTACTCCAG 
TTTGTGAAAC GTGGCGGGGG CTTGCTCATA GGAGGCCAAG CCTGGGATTG GGATAACCTG 
GGTGAAGATG ATAGAGTACT GTTCTCCTTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT 
GTGTACTTCA CTGACAACAA AGCGGACACG AATTGCTATA AAGTCTCCAA GAAGATGCCT 
AAGATACCCA TCTTAGTTCG TTGTGAAGAT GACCTCTCAG AAGACAGAGA TGAGCTTCTA 
CATGGCATCT CAGACCTGGA TATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTG 
CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCCACC ATGGCTGTGT GATAGCTGCT 
GCCCGCTATG GCCGGGGACG GGTGGTTGTA ACTGGTCATA AGGTATTATT CACTGTGGGT 
AAACTCGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTAG ATGGCGGCCG CAGAGGCAAG 
ATTGTGGTAC AGACCGAGCT GAGAACACTA AGTGGTCTGC TTGCAGTAGG GGGTATAGAC 
ACCAGCATTG AGCCCCAACT GACCAGTGAC GCAAGTGTCT ATTGCTTCGA ACCCACAAGT 
GATGTGGGAG TTAAGGAACT ACAGGAGTTT GTAGCTGAGG GTGGTGGGCT GTTTGTTGGA 
ACCCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCACTGGC TCGATTCCCA 
GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAAAGCCT CAATCCAGGA 
CCTTTCCATA TCCCAAAAGC AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTGGCAGAG 
TTCCAGGTGA TAATGGGCAG GAAAAGAGGA AATGTGGAGA AGGGCTGGCT AGCCAAGCTG 
GGCCCAGATG GGGCAGCTTT CCTGCAGATT CCTGCAGAGG AGATCCCTGC TTACATGTCC 
GTGCACAGAC TCCTGCGGAA GCTGCTGAGT CGCTACCGGC TTCCAGTGGC AACCAGAGAG 
AACCCGGTCA TCAATGACTG TTGTAGGGGG GCCATGCTTT CCCTGGCCAC AGGTCTGGCC 
CACTCTGGAA GTGACCTCTC ACTGCTAATC CCAGAAATTG AAGATGTGTA CAGCAGCTGC 
TACCTACGCC CCTCAGAGTC TCCCATCACC GTGGATGTCA ACTGCACCAA TCCAGGCACC 
CGATACTGCT GGATGAGCAC TGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTTTCC 
TTGCCTGAAG ATGCTGCCTC TGCTGATCTG AAGATACAAA TTGGCTGTCA CACTGATGAC 
CTGACCAGGG CCAGCAAGCT CTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAT 
AAACCCATGA AATCAATCAA CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG 
AATAGCAAAC TGGGTACTGT GCCCCTCACA GTGAAGGGAG CCGTTCGTGC CCCATTCTAC 
AAGCTAGGGG AGACATCCAA GGAAGAGTGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCG 
TGGGGAGAAC TGGCCACTGA CAACCTCATC TTGACTGTGC CTACTGCAAA TCTTCGAACT 
CTGGAGAACC CTGAGCCACT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCTAAG 
TTGGGAGGCG AGCCCTTCCC TTTGCGGCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC 
TCAGTAGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG 
CTCATTAGTG AGAAGCTCAT CCGGACCAAG GGACTGTGGG GTCCTGTGCA TGAGCTGGGC 
CGCAACCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACATGCAAC 
CTGTGGTGTG TTTATGTGCA TGAAACAGTT CTGGGCATAC CTCGAAGCCG TGCCAACATT 
GCGCTATGGC CCCCTGTTCG GGAGAAGAGA GTCCGAATGT ATTTAAGCAA GGGACCCAAT 
GTGAAAAACT GGAATGCCTG GACTGCGTTG GAAACGTACC TACAGCTTCA AGAGGCCTTT 
GGTTGGGAGC CATTTATTCG TCTCTTCACT GAGTACAGAA ACCAGACAAC GAACTCGCCC 
ACAGACAACG TCAACAAAAT GAATCTCTGG GTCAAGATGT TCTCCCAGCA AGTGCAGAAG 
AATCTGGCTC CGTTCTTTGA GGCCTGGGCC TGGCCTATCC AGAAAGAAGT GGCTACAAGC 
TTAGCCTATC TGCCTGAATG GAAAGAAAAT ATAATGAAAT TGTATCTTCT CACACAGTTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003749787.1
CDS	171..2933
Translation	MATPSATFEALMNGVTSWDIPDDSVPCELLLIGEASFPIMVNDVGQVLIAASSYGRGRMVVASHEDFLVESQLFVFLVNAVGWLRSSSESTIGVHSSLAPLVKILESCGIESKIEPEVNDSLGVYCIDAYNETMTDKLLQFVKRGGGLLIGGQAWDWDNLGEDDRVLFSFPGNLVTSVAGVYFTDNKADTNCYKVSKKMPKIPILVRCEDDLSEDRDELLHGISDLDITNSDCFPSQLLVHGSLAFPLGLDTHHGCVIAAARYGRGRVVVTGHKVLFTVGKLGPFLLNAVRWLDGGRRGKIVVQTELRTLSGLLAVGGIDTSIEPQLTSDASVYCFEPTSDVGVKELQEFVAEGGGLFVGTQAWWWAFKNPGVSPLARFPGNLLLNAFGISITSQSLNPGPFHIPKAGTRTYHFRSTLAEFQVIMGRKRGNVEKGWLAKLGPDGAAFLQIPAEEIPAYMSVHRLLRKLLSRYRLPVATRENPVINDCCRGAMLSLATGLAHSGSDLSLLIPEIEDVYSSCYLRPSESPITVDVNCTNPGTRYCWMSTGLYIPGRQVIDVSLPEDAASADLKIQIGCHTDDLTRASKLFRGPLVINRCCLDKPMKSINCLWGGLLYIIVPQNSKLGTVPLTVKGAVRAPFYKLGETSKEEWKRRLLEYPGPWGELATDNLILTVPTANLRTLENPEPLLRLWDEVMQAVAKLGGEPFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQELISEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCNLWCVYVHETVLGIPRSRANIALWPPVREKRVRMYLSKGPNVKNWNAWTALETYLQLQEAFGWEPFIRLFTEYRNQTTNSPTDNVNKMNLWVKMFSQQVQKNLAPFFEAWAWPIQKEVATSLAYLPEWKENIMKLYLLTQL

Target ORF information:

RefSeq Version	XM_003749739.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus TRPM8 channel-associated factor 1 (Tcaf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003749739.4

ORF Insert Sequence:

ATGGCAACTC CATCTGCTAC CTTTGAGGCG CTTATGAATG GAGTAACAAG TTGGGATATC 
CCTGATGATT CCGTTCCATG TGAACTGCTT CTTATTGGAG AGGCTTCTTT TCCTATAATG 
GTGAATGATG TAGGCCAGGT CCTCATTGCT GCCTCTTCCT ATGGCAGAGG TCGCATGGTG 
GTAGCATCTC ATGAGGACTT CTTGGTGGAA AGCCAGCTCT TTGTCTTTCT TGTCAATGCT 
GTTGGATGGC TTCGTTCCTC TTCTGAGTCA ACCATTGGTG TGCACTCATC TTTAGCACCA 
TTGGTGAAAA TCCTCGAGAG CTGTGGAATA GAGTCAAAGA TTGAACCAGA AGTGAATGAC 
TCTCTGGGGG TTTACTGCAT CGATGCCTAC AATGAAACCA TGACGGATAA GCTACTCCAG 
TTTGTGAAAC GTGGCGGGGG CTTGCTCATA GGAGGCCAAG CCTGGGATTG GGATAACCTG 
GGTGAAGATG ATAGAGTACT GTTCTCCTTT CCTGGGAATC TGGTGACCAG TGTGGCTGGT 
GTGTACTTCA CTGACAACAA AGCGGACACG AATTGCTATA AAGTCTCCAA GAAGATGCCT 
AAGATACCCA TCTTAGTTCG TTGTGAAGAT GACCTCTCAG AAGACAGAGA TGAGCTTCTA 
CATGGCATCT CAGACCTGGA TATCACCAAC TCGGACTGCT TCCCATCCCA GCTACTAGTG 
CATGGGTCTT TGGCTTTTCC TTTGGGGCTA GATACCCACC ATGGCTGTGT GATAGCTGCT 
GCCCGCTATG GCCGGGGACG GGTGGTTGTA ACTGGTCATA AGGTATTATT CACTGTGGGT 
AAACTCGGCC CCTTTCTGCT CAATGCTGTC CGCTGGCTAG ATGGCGGCCG CAGAGGCAAG 
ATTGTGGTAC AGACCGAGCT GAGAACACTA AGTGGTCTGC TTGCAGTAGG GGGTATAGAC 
ACCAGCATTG AGCCCCAACT GACCAGTGAC GCAAGTGTCT ATTGCTTCGA ACCCACAAGT 
GATGTGGGAG TTAAGGAACT ACAGGAGTTT GTAGCTGAGG GTGGTGGGCT GTTTGTTGGA 
ACCCAAGCCT GGTGGTGGGC TTTTAAGAAC CCAGGAGTGT CCCCACTGGC TCGATTCCCA 
GGAAACCTGC TCCTCAATGC CTTTGGTATC AGCATCACAA GCCAAAGCCT CAATCCAGGA 
CCTTTCCATA TCCCAAAAGC AGGAACAAGG ACCTATCACT TCCGCTCTAC CCTGGCAGAG 
TTCCAGGTGA TAATGGGCAG GAAAAGAGGA AATGTGGAGA AGGGCTGGCT AGCCAAGCTG 
GGCCCAGATG GGGCAGCTTT CCTGCAGATT CCTGCAGAGG AGATCCCTGC TTACATGTCC 
GTGCACAGAC TCCTGCGGAA GCTGCTGAGT CGCTACCGGC TTCCAGTGGC AACCAGAGAG 
AACCCGGTCA TCAATGACTG TTGTAGGGGG GCCATGCTTT CCCTGGCCAC AGGTCTGGCC 
CACTCTGGAA GTGACCTCTC ACTGCTAATC CCAGAAATTG AAGATGTGTA CAGCAGCTGC 
TACCTACGCC CCTCAGAGTC TCCCATCACC GTGGATGTCA ACTGCACCAA TCCAGGCACC 
CGATACTGCT GGATGAGCAC TGGACTCTAC ATACCTGGAA GGCAAGTCAT AGATGTTTCC 
TTGCCTGAAG ATGCTGCCTC TGCTGATCTG AAGATACAAA TTGGCTGTCA CACTGATGAC 
CTGACCAGGG CCAGCAAGCT CTTCCGAGGC CCCCTTGTGA TTAACCGGTG TTGCTTGGAT 
AAACCCATGA AATCAATCAA CTGCCTCTGG GGTGGTCTCC TCTATATCAT TGTGCCTCAG 
AATAGCAAAC TGGGTACTGT GCCCCTCACA GTGAAGGGAG CCGTTCGTGC CCCATTCTAC 
AAGCTAGGGG AGACATCCAA GGAAGAGTGG AAGAGGCGTC TCCTGGAATA TCCAGGTCCG 
TGGGGAGAAC TGGCCACTGA CAACCTCATC TTGACTGTGC CTACTGCAAA TCTTCGAACT 
CTGGAGAACC CTGAGCCACT GCTCCGCCTC TGGGATGAGG TGATGCAGGC TGTGGCTAAG 
TTGGGAGGCG AGCCCTTCCC TTTGCGGCTG CCACAGAGGA TAGTGGCTGA TGTTCAGATC 
TCAGTAGGCT GGATGCATGC AGGGTACCCC ATCATGTGCC ATCTGGAGTC AGTGCAGGAG 
CTCATTAGTG AGAAGCTCAT CCGGACCAAG GGACTGTGGG GTCCTGTGCA TGAGCTGGGC 
CGCAACCAGC AGAGGCAGGA GTGGGAGTTC CCCCCACATA CTACGGAAGC CACATGCAAC 
CTGTGGTGTG TTTATGTGCA TGAAACAGTT CTGGGCATAC CTCGAAGCCG TGCCAACATT 
GCGCTATGGC CCCCTGTTCG GGAGAAGAGA GTCCGAATGT ATTTAAGCAA GGGACCCAAT 
GTGAAAAACT GGAATGCCTG GACTGCGTTG GAAACGTACC TACAGCTTCA AGAGGCCTTT 
GGTTGGGAGC CATTTATTCG TCTCTTCACT GAGTACAGAA ACCAGACAAC GAACTCGCCC 
ACAGACAACG TCAACAAAAT GAATCTCTGG GTCAAGATGT TCTCCCAGCA AGTGCAGAAG 
AATCTGGCTC CGTTCTTTGA GGCCTGGGCC TGGCCTATCC AGAAAGAAGT GGCTACAAGC 
TTAGCCTATC TGCCTGAATG GAAAGAAAAT ATAATGAAAT TGTATCTTCT CACACAGTTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Tcaf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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