POPTR_0002s15020g cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa)

Gene
Clones
gRNAs

Summary

Gene Symbol	POPTR_0002s15020g
Entrez Gene ID	7494008
Full Name	hypothetical protein
Synonyms	POPTRDRAFT_644347,POPTRDRAFT_644349
Gene Type	protein-coding
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002301262.2	XP_002301298.2	hypothetical protein
XM_024595681.1	XP_024451449.1	brefeldin A-inhibited guanine nucleotide-exchange protein 2 isoform X2
XM_002301261.2	XP_002301297.2	hypothetical protein
XM_002301262.3	XP_002301298.3	brefeldin A-inhibited guanine nucleotide-exchange protein 2 isoform X1
XM_024595680.1	XP_024451448.1	brefeldin A-inhibited guanine nucleotide-exchange protein 2 isoform X2
XM_002301263.2	XP_002301299.2	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, Déjardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasjärvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Leplé JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouzé P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POPTR_0002s15020g gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POPTR_0002s15020g cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPa06519	XM_002301261.2 Latest version!	Populus trichocarpa hypothetical protein (POPTR_0002s15020g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPa06521	XM_002301263.2 Latest version!	Populus trichocarpa hypothetical protein (POPTR_0002s15020g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPa424978	XM_024595680.1 Latest version!	Populus trichocarpa brefeldin A-inhibited guanine nucleotide-exchange protein 2 (LOC7494008), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPa424978	XM_024595681.1 Latest version!	Populus trichocarpa brefeldin A-inhibited guanine nucleotide-exchange protein 2 (LOC7494008), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPa06520	XM_002301262.2 Latest version!	Populus trichocarpa hypothetical protein (POPTR_0002s15020g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPa424977	XM_002301262.3 Latest version!	Populus trichocarpa brefeldin A-inhibited guanine nucleotide-exchange protein 2 (LOC7494008), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POPTR_0002s15020g ( XM_002301261.2 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_002301297.2 Populus trichocarpa hypothetical protein (POPTR_0002s15020g) mRNA, complete cds.

Price & Availability↑

CloneID	OPa06519
Clone ID Related Accession (Same CDS sequence)	XM_002301261.2
Accession Version	XM_002301261.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1388332800000
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008468). On Dec 16, 2013 this sequence version replaced XM_002301261.1. COMPLETENESS: incomplete on the 5' end.

ATGGCTTCCT CGGAAGCCGA TTCCCGGTTA AGCCAAGTGG TATCCCCTGC TTTAGAAAAA 
ATCGTCAAAA ACGCATCGTG GCGTAAACAC TCGAAATTAG CACACGAATG CAAATCCGTC 
CTCGAGATTC TCACTTCTCG AAAACCCCAA CAACAACATC CTCCGACTTC CCCGTCGGAC 
GACTCCTCAT CCGAATCCTC TCTCCCTGGC CCACTCCACG ACGGCGGGTC CATCGAGTAC 
TCCCTCGCCG AATCTGAATC TATCCTCAGC CCTCTGATCA ACGCGTGCAA CACTCAATTC 
CTCAAGATCG TCGATCCAGC CGTTGATTGC ATCCAGAAAT TGATCGCGCA TGGGTACCTA 
CGTGGCGAGG CGGACTCCAC CGGAGGTACT GAAGCGAAAT TGCTAGCTAA GTTGATTGAA 
TCTGTGTGTA AATGTTATGA TTTAGGGGAT GATGGTGCTG AATTGCTGGT TTTGAAAACC 
CTATTATCAG CCGTTACGTC GATTTCGTTG AGAATTCATG GAGATTGTTT GTTACAAATA 
GTGAGAACTT GTTATGACAT TTATTTAGGG AGTAAAAATG TAATTAATCA AACGACGGCG 
AAAGCGTCTT TGATTCAAAT GTTAGTTATT GTGTTTAGGA GAATGGAGGC TGATTCTTCA 
ACGGTTCCGG TTCAGCCGAT TGTTGTGGCT GAATTAATGG AACCAGTGGA GAAAACGGAT 
GTCGATGGAT CAATGGCGGT GTTTGTTCAA GGTTTTATAA CGAAAATTAT GCAGGATATA 
GATGGGGTTT TTAATCCGGG GACACCCAGT AAGTCTTCGA TGACAGTGGC ACATGATGGG 
GCTTTCGAGA CCACTACTGG TACGGTGGAG AGTACAAATC CGGCAGATTT GCTTGATTCG 
ACGGATAAGG ATATGTTAGA TGCCAAATAT TGGGAAATTA GTATGTATAA GACGGCTTTG 
GAAGGCAGGA AAGGGGAGTT GGCTGATGGG GAAGGGGAGA GGGAGGATGA TTTGGAAGTG 
CAGATTGGGA ATAAGTTGAG GAGAGATGCA TTTTTGGTGT TTCGAGCACT TTGTAAGTTA 
TCTATGAAAA CTCCGCCCAA AGAAGCATTG GCGGATCCAC AGTTGATGAG AGGGAAGATT 
GTTGCATTGG AGTTATTGAA GATTTTGTTG GAGAATGCTG GTGCTGTGTT TAGGACTAGT 
GACAGGTTCT TAGGTGCCAT CAAGCAATAT CTATGTCTTT CACTGTTGAA GAACAGCTCG 
TCAAGTCTTA TGATCATTTT CCAGCTTTCT TGCTCCATTT TCATTAGTTT GGTTTCAAGG 
TTCAGAGCTG GACTGAAAGC AGAAATTGGA GTATTTTTTC CTATGATTGT TCTCAGGATT 
CTTGAGAATG TTGTTCAACC TAATTTTCAG CAGAAGATTA TAGTACTTCG GTTTCTAGAT 
AAGCTCTGTG TTGATTCCCA GATCTTGGTA GACATTTTTA TTAATTATGA CTGTGATATC 
AACTCATCAA ACATATTTGA GAGAATGGTC AATGGACTTC TTAAAACTGC TCAAGGTGCC 
CTGCCTGGTA CTGCCACTAC ACTGGTGCCA CCTCAGGAGG TGACCATGAA ACTTGAAGCT 
ATGAAGTCCT TAGTGGCTAT TCTGAAATCA ATGGGAGACT GGATGAATAA ACAATTGCGC 
ATTCCCGATC CTCATTCTGC CAAGAAATCT GATGCAGCCG AAAACAGTCC TGGACCTGGA 
AGTCTTCCCA TGACAAATGG TAATGGGGAT GAGCCTGTTG AAGGATCAGA TTCTCATTCT 
GAAACCTCAA CTGAAGCATC GGATGTTTCA GCTATTGAGC AACGACGGGC TTACAAGCTG 
GAATTTCAGG AAGGTATATC TCTTTTCAAT CGGAAGCCTA AGAAAGGAAT TGAATTTCTA 
ATCAATGCAA ATAAAGTGGG GAATTCAGCA GAAGAGATAG CAGCCTTTCT TAAAAACGCA 
TCTGGCTTGA ACAAGACTCT GATTGGCGAT TACCTTGGGG AAAGAGAAGA TTTTTCACTG 
AAAGTAATGC ATGCTTATGT AGATTCTTTT GACTTTCGCG GCCTGGAGTT CGATGAGGCT 
ATCAGAGTCT TTCTACAGGG CTTTAGGTTA CCAGGTGAGG CACAAAAGAT TGATCGAATT 
ATGGAGAAGT TTGCTGAACG TTACTGCAAA TGTAACCCAA AGGTTTTTAG CAGTGCTGAC 
ACAGCCTATG TCCTTGCTTA CTCCGTGATA CTGCTCAACA CTGATGCTCA TAATCCCATG 
GTGAAGAATA AGATGTCAGC TGATGACTTC ATAAGAAACA ACCGTGGCAT TGATGATGGG 
AAAGATCTAC CTGAGGAGTA CTTGAGATCT TTGTTTGAAA GAATATCAAA AAATGAGATC 
AAAATGAAAG AATATGATTT AGCTCTCCAA CAAAAGCAGT CTTTGAATTC AAACAGAGTT 
TTAGGCTTGG ATAGTATCCT GAACATTGTG ATCCGCAAGC GGGGTGAAGA AAAGAATATG 
GAAACAAGTG ATGATCTTAT CAGGCACATG CAGGAACAAT TTAAAGAAAA AGCTCGAAAA 
TCTGAGTCAG TTTATTATGC TGCAACAGAT GTGGTGATTC TTAGATTCAT GATTGAGGTA 
TGCTGGGCTC CTATGCTGGC AGCCTTCAGT GTGCCTCTTG ACCAAAGTGA TGATGAAGTG 
GTGATAGCTT TGTGTCTTGA AGGTATCCGC TATGCAATCC ATGTTACTGC TGTGATGTCC 
ATGAAGACTC ATAGAGATGC TTTTGTGACT TCATTAGCAA AGTTTACATC GCTCCACTCC 
CCTGCTGATA TCAAGCAGAA AAATATTGAT GCTATCAAGG CAATAGTTAC AATTGCAGAT 
GAAGATGGGA ATTATTTGCA AGAAGCTTGG GAACATATTT TGACATGTGT CTCACGCTTT 
GAACATTTGC ATCTCCTGGG AGAGGGTGCT CCTCCAGATG CCACTTTTTT TGCTTTTCCC 
CAAAATAACT CTGAAAAGTC AAAACAAAGC AAGTCAACCA TCCTCCCTGT TCTGAAAAAG 
AAGGGACCCG GGAGGATGCA GCATGCAGCT GCTAGTGTGC TGAGAGGTTC ATATGATAGT 
GCTGGTATTG GTGGAAACGC TGCTGGGGCT GTCACAAGTG AGCAGATGAA CAATTTAGTT 
TCCAACTTAA ACAAGTTAGA GCAAGTTGGC AGCTCTGAGA TGAACCGCAT CTTCACACGG 
AGCCAAAAGT TAAACAGTGA GGCAATAATT GACTTTGTGA AGGCTCTTTG CAAGGTTTCT 
GTGGAGGAAT TGAGATCAGC TTCTGATCCA CGGGTCTTTA GCCTTACAAA GATTGTTGAG 
ATTGCGCACT TTAACATGAA TCGCATCAGG CTTGTATGGT CAAGCATCTG GCATGTGCTT 
TCTGATTTCT TTGTAACCAT TGGCTGTTCT GAAAACCTTT CCATTGCAAT TTTTGCAATG 
GACTCTTTAC GCCAATTATC AATGAAATTC TTAGACCGAG AAGAGTTGGC AAACTATAAT 
TTTCAGAATG AGTTTATGAA GCCATTTGTT ATTGTTATGC GGAAGAGTAA TGCTGTTGAA 
ATCAGAGAAT TGATTATCAG ATGTGTCTCG CAAATGGTCC TGTCTCGTGT CAATAATGTT 
AAATCTGGAT GGAAGAGCAT GTTCATGGTA TTGAAATCTT CTGATTTTCA ACCTTACTTA 
TGGTTTTCAC AGCAGCAGCT TATGATGACC ACAAAAACAT TGTACTCTTG GCCTTTGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002301297.2
CDS	1..3783
Translation	MASSEADSRLSQVVSPALEKIVKNASWRKHSKLAHECKSVLEILTSRKPQQQHPPTSPSDDSSSESSLPGPLHDGGSIEYSLAESESILSPLINACNTQFLKIVDPAVDCIQKLIAHGYLRGEADSTGGTEAKLLAKLIESVCKCYDLGDDGAELLVLKTLLSAVTSISLRIHGDCLLQIVRTCYDIYLGSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPVEKTDVDGSMAVFVQGFITKIMQDIDGVFNPGTPSKSSMTVAHDGAFETTTGTVESTNPADLLDSTDKDMLDAKYWEISMYKTALEGRKGELADGEGEREDDLEVQIGNKLRRDAFLVFRALCKLSMKTPPKEALADPQLMRGKIVALELLKILLENAGAVFRTSDRFLGAIKQYLCLSLLKNSSSSLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRILENVVQPNFQQKIIVLRFLDKLCVDSQILVDIFINYDCDINSSNIFERMVNGLLKTAQGALPGTATTLVPPQEVTMKLEAMKSLVAILKSMGDWMNKQLRIPDPHSAKKSDAAENSPGPGSLPMTNGNGDEPVEGSDSHSETSTEASDVSAIEQRRAYKLEFQEGISLFNRKPKKGIEFLINANKVGNSAEEIAAFLKNASGLNKTLIGDYLGEREDFSLKVMHAYVDSFDFRGLEFDEAIRVFLQGFRLPGEAQKIDRIMEKFAERYCKCNPKVFSSADTAYVLAYSVILLNTDAHNPMVKNKMSADDFIRNNRGIDDGKDLPEEYLRSLFERISKNEIKMKEYDLALQQKQSLNSNRVLGLDSILNIVIRKRGEEKNMETSDDLIRHMQEQFKEKARKSESVYYAATDVVILRFMIEVCWAPMLAAFSVPLDQSDDEVVIALCLEGIRYAIHVTAVMSMKTHRDAFVTSLAKFTSLHSPADIKQKNIDAIKAIVTIADEDGNYLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNNSEKSKQSKSTILPVLKKKGPGRMQHAAASVLRGSYDSAGIGGNAAGAVTSEQMNNLVSNLNKLEQVGSSEMNRIFTRSQKLNSEAIIDFVKALCKVSVEELRSASDPRVFSLTKIVEIAHFNMNRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFMKPFVIVMRKSNAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVLKSSDFQPYLWFSQQQLMMTTKTLYSWPLK

Target ORF information:

RefSeq Version	XM_002301261.2
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa hypothetical protein (POPTR_0002s15020g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002301261.2

ORF Insert Sequence:

ATGGCTTCCT CGGAAGCCGA TTCCCGGTTA AGCCAAGTGG TATCCCCTGC TTTAGAAAAA 
ATCGTCAAAA ACGCATCGTG GCGTAAACAC TCGAAATTAG CACACGAATG CAAATCCGTC 
CTCGAGATTC TCACTTCTCG AAAACCCCAA CAACAACATC CTCCGACTTC CCCGTCGGAC 
GACTCCTCAT CCGAATCCTC TCTCCCTGGC CCACTCCACG ACGGCGGGTC CATCGAGTAC 
TCCCTCGCCG AATCTGAATC TATCCTCAGC CCTCTGATCA ACGCGTGCAA CACTCAATTC 
CTCAAGATCG TCGATCCAGC CGTTGATTGC ATCCAGAAAT TGATCGCGCA TGGGTACCTA 
CGTGGCGAGG CGGACTCCAC CGGAGGTACT GAAGCGAAAT TGCTAGCTAA GTTGATTGAA 
TCTGTGTGTA AATGTTATGA TTTAGGGGAT GATGGTGCTG AATTGCTGGT TTTGAAAACC 
CTATTATCAG CCGTTACGTC GATTTCGTTG AGAATTCATG GAGATTGTTT GTTACAAATA 
GTGAGAACTT GTTATGACAT TTATTTAGGG AGTAAAAATG TAATTAATCA AACGACGGCG 
AAAGCGTCTT TGATTCAAAT GTTAGTTATT GTGTTTAGGA GAATGGAGGC TGATTCTTCA 
ACGGTTCCGG TTCAGCCGAT TGTTGTGGCT GAATTAATGG AACCAGTGGA GAAAACGGAT 
GTCGATGGAT CAATGGCGGT GTTTGTTCAA GGTTTTATAA CGAAAATTAT GCAGGATATA 
GATGGGGTTT TTAATCCGGG GACACCCAGT AAGTCTTCGA TGACAGTGGC ACATGATGGG 
GCTTTCGAGA CCACTACTGG TACGGTGGAG AGTACAAATC CGGCAGATTT GCTTGATTCG 
ACGGATAAGG ATATGTTAGA TGCCAAATAT TGGGAAATTA GTATGTATAA GACGGCTTTG 
GAAGGCAGGA AAGGGGAGTT GGCTGATGGG GAAGGGGAGA GGGAGGATGA TTTGGAAGTG 
CAGATTGGGA ATAAGTTGAG GAGAGATGCA TTTTTGGTGT TTCGAGCACT TTGTAAGTTA 
TCTATGAAAA CTCCGCCCAA AGAAGCATTG GCGGATCCAC AGTTGATGAG AGGGAAGATT 
GTTGCATTGG AGTTATTGAA GATTTTGTTG GAGAATGCTG GTGCTGTGTT TAGGACTAGT 
GACAGGTTCT TAGGTGCCAT CAAGCAATAT CTATGTCTTT CACTGTTGAA GAACAGCTCG 
TCAAGTCTTA TGATCATTTT CCAGCTTTCT TGCTCCATTT TCATTAGTTT GGTTTCAAGG 
TTCAGAGCTG GACTGAAAGC AGAAATTGGA GTATTTTTTC CTATGATTGT TCTCAGGATT 
CTTGAGAATG TTGTTCAACC TAATTTTCAG CAGAAGATTA TAGTACTTCG GTTTCTAGAT 
AAGCTCTGTG TTGATTCCCA GATCTTGGTA GACATTTTTA TTAATTATGA CTGTGATATC 
AACTCATCAA ACATATTTGA GAGAATGGTC AATGGACTTC TTAAAACTGC TCAAGGTGCC 
CTGCCTGGTA CTGCCACTAC ACTGGTGCCA CCTCAGGAGG TGACCATGAA ACTTGAAGCT 
ATGAAGTCCT TAGTGGCTAT TCTGAAATCA ATGGGAGACT GGATGAATAA ACAATTGCGC 
ATTCCCGATC CTCATTCTGC CAAGAAATCT GATGCAGCCG AAAACAGTCC TGGACCTGGA 
AGTCTTCCCA TGACAAATGG TAATGGGGAT GAGCCTGTTG AAGGATCAGA TTCTCATTCT 
GAAACCTCAA CTGAAGCATC GGATGTTTCA GCTATTGAGC AACGACGGGC TTACAAGCTG 
GAATTTCAGG AAGGTATATC TCTTTTCAAT CGGAAGCCTA AGAAAGGAAT TGAATTTCTA 
ATCAATGCAA ATAAAGTGGG GAATTCAGCA GAAGAGATAG CAGCCTTTCT TAAAAACGCA 
TCTGGCTTGA ACAAGACTCT GATTGGCGAT TACCTTGGGG AAAGAGAAGA TTTTTCACTG 
AAAGTAATGC ATGCTTATGT AGATTCTTTT GACTTTCGCG GCCTGGAGTT CGATGAGGCT 
ATCAGAGTCT TTCTACAGGG CTTTAGGTTA CCAGGTGAGG CACAAAAGAT TGATCGAATT 
ATGGAGAAGT TTGCTGAACG TTACTGCAAA TGTAACCCAA AGGTTTTTAG CAGTGCTGAC 
ACAGCCTATG TCCTTGCTTA CTCCGTGATA CTGCTCAACA CTGATGCTCA TAATCCCATG 
GTGAAGAATA AGATGTCAGC TGATGACTTC ATAAGAAACA ACCGTGGCAT TGATGATGGG 
AAAGATCTAC CTGAGGAGTA CTTGAGATCT TTGTTTGAAA GAATATCAAA AAATGAGATC 
AAAATGAAAG AATATGATTT AGCTCTCCAA CAAAAGCAGT CTTTGAATTC AAACAGAGTT 
TTAGGCTTGG ATAGTATCCT GAACATTGTG ATCCGCAAGC GGGGTGAAGA AAAGAATATG 
GAAACAAGTG ATGATCTTAT CAGGCACATG CAGGAACAAT TTAAAGAAAA AGCTCGAAAA 
TCTGAGTCAG TTTATTATGC TGCAACAGAT GTGGTGATTC TTAGATTCAT GATTGAGGTA 
TGCTGGGCTC CTATGCTGGC AGCCTTCAGT GTGCCTCTTG ACCAAAGTGA TGATGAAGTG 
GTGATAGCTT TGTGTCTTGA AGGTATCCGC TATGCAATCC ATGTTACTGC TGTGATGTCC 
ATGAAGACTC ATAGAGATGC TTTTGTGACT TCATTAGCAA AGTTTACATC GCTCCACTCC 
CCTGCTGATA TCAAGCAGAA AAATATTGAT GCTATCAAGG CAATAGTTAC AATTGCAGAT 
GAAGATGGGA ATTATTTGCA AGAAGCTTGG GAACATATTT TGACATGTGT CTCACGCTTT 
GAACATTTGC ATCTCCTGGG AGAGGGTGCT CCTCCAGATG CCACTTTTTT TGCTTTTCCC 
CAAAATAACT CTGAAAAGTC AAAACAAAGC AAGTCAACCA TCCTCCCTGT TCTGAAAAAG 
AAGGGACCCG GGAGGATGCA GCATGCAGCT GCTAGTGTGC TGAGAGGTTC ATATGATAGT 
GCTGGTATTG GTGGAAACGC TGCTGGGGCT GTCACAAGTG AGCAGATGAA CAATTTAGTT 
TCCAACTTAA ACAAGTTAGA GCAAGTTGGC AGCTCTGAGA TGAACCGCAT CTTCACACGG 
AGCCAAAAGT TAAACAGTGA GGCAATAATT GACTTTGTGA AGGCTCTTTG CAAGGTTTCT 
GTGGAGGAAT TGAGATCAGC TTCTGATCCA CGGGTCTTTA GCCTTACAAA GATTGTTGAG 
ATTGCGCACT TTAACATGAA TCGCATCAGG CTTGTATGGT CAAGCATCTG GCATGTGCTT 
TCTGATTTCT TTGTAACCAT TGGCTGTTCT GAAAACCTTT CCATTGCAAT TTTTGCAATG 
GACTCTTTAC GCCAATTATC AATGAAATTC TTAGACCGAG AAGAGTTGGC AAACTATAAT 
TTTCAGAATG AGTTTATGAA GCCATTTGTT ATTGTTATGC GGAAGAGTAA TGCTGTTGAA 
ATCAGAGAAT TGATTATCAG ATGTGTCTCG CAAATGGTCC TGTCTCGTGT CAATAATGTT 
AAATCTGGAT GGAAGAGCAT GTTCATGGTA TTGAAATCTT CTGATTTTCA ACCTTACTTA 
TGGTTTTCAC AGCAGCAGCT TATGATGACC ACAAAAACAT TGTACTCTTG GCCTTTGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0002s15020g ( XM_002301263.2 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_002301299.2 Populus trichocarpa hypothetical protein (POPTR_0002s15020g) mRNA, complete cds.

Price & Availability↑

CloneID	OPa06521
Clone ID Related Accession (Same CDS sequence)	XM_002301263.2
Accession Version	XM_002301263.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1388332800000
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008468). On Dec 16, 2013 this sequence version replaced XM_002301263.1.

ATGATCATTT TCCAGCTTTC TTGCTCCATT TTCATTAGTT TGGTTTCAAG GTTCAGAGCT 
GGACTGAAAG CAGAAATTGG AGTATTTTTT CCTATGATTG TTCTCAGGAT TCTTGAGAAT 
GTTGTTCAAC CTAATTTTCA GCAGAAGATT ATAGTACTTC GGTTTCTAGA TAAGCTCTGT 
GTTGATTCCC AGATCTTGGT AGACATTTTT ATTAATTATG ACTGTGATAT CAACTCATCA 
AACATATTTG AGAGAATGGT CAATGGACTT CTTAAAACTG CTCAAGGTGC CCTGCCTGGT 
ACTGCCACTA CACTGGTGCC ACCTCAGGAG GTGACCATGA AACTTGAAGC TATGAAGTCC 
TTAGTGGCTA TTCTGAAATC AATGGGAGAC TGGATGAATA AACAATTGCG CATTCCCGAT 
CCTCATTCTG CCAAGAAATC TGATGCAGCC GAAAACAGTC CTGGACCTGG AAGTCTTCCC 
ATGACAAATG GTAATGGGGA TGAGCCTGTT GAAGGATCAG ATTCTCATTC TGAAACCTCA 
ACTGAAGCAT CGGATGTTTC AGCTATTGAG CAACGACGGG CTTACAAGCT GGAATTTCAG 
GAAGGTATAT CTCTTTTCAA TCGGAAGCCT AAGAAAGGAA TTGAATTTCT AATCAATGCA 
AATAAAGTGG GGAATTCAGC AGAAGAGATA GCAGCCTTTC TTAAAAACGC ATCTGGCTTG 
AACAAGACTC TGATTGGCGA TTACCTTGGG GAAAGAGAAG ATTTTTCACT GAAAGTAATG 
CATGCTTATG TAGATTCTTT TGACTTTCGC GGCCTGGAGT TCGATGAGGC TATCAGAGTC 
TTTCTACAGG GCTTTAGGTT ACCAGGTGAG GCACAAAAGA TTGATCGAAT TATGGAGAAG 
TTTGCTGAAC GTTACTGCAA ATGTAACCCA AAGGTTTTTA GCAGTGCTGA CACAGCCTAT 
GTCCTTGCTT ACTCCGTGAT ACTGCTCAAC ACTGATGCTC ATAATCCCAT GGTGAAGAAT 
AAGATGTCAG CTGATGACTT CATAAGAAAC AACCGTGGCA TTGATGATGG GAAAGATCTA 
CCTGAGGAGT ACTTGAGATC TTTGTTTGAA AGAATATCAA AAAATGAGAT CAAAATGAAA 
GAATATGATT TAGCTCTCCA ACAAAAGCAG TCTTTGAATT CAAACAGAGT TTTAGGCTTG 
GATAGTATCC TGAACATTGT GATCCGCAAG CGGGGTGAAG AAAAGAATAT GGAAACAAGT 
GATGATCTTA TCAGGCACAT GCAGGAACAA TTTAAAGAAA AAGCTCGAAA ATCTGAGTCA 
GTTTATTATG CTGCAACAGA TGTGGTGATT CTTAGATTCA TGATTGAGGT ATGCTGGGCT 
CCTATGCTGG CAGCCTTCAG TGTGCCTCTT GACCAAAGTG ATGATGAAGT GGTGATAGCT 
TTGTGTCTTG AAGGTATCCG CTATGCAATC CATGTTACTG CTGTGATGTC CATGAAGACT 
CATAGAGATG CTTTTGTGAC TTCATTAGCA AAGTTTACAT CGCTCCACTC CCCTGCTGAT 
ATCAAGCAGA AAAATATTGA TGCTATCAAG GCAATAGTTA CAATTGCAGA TGAAGATGGG 
AATTATTTGC AAGAAGCTTG GGAACATATT TTGACATGTG TCTCACGCTT TGAACATTTG 
CATCTCCTGG GAGAGGGTGC TCCTCCAGAT GCCACTTTTT TTGCTTTTCC CCAAAATAAC 
TCTGAAAAGT CAAAACAAAG CAAGTCAACC ATCCTCCCTG TTCTGAAAAA GAAGGGACCC 
GGGAGGATGC AGCATGCAGC TGCTAGTGTG CTGAGAGGTT CATATGATAG TGCTGGTATT 
GGTGGAAACG CTGCTGGGGC TGTCACAAGT GAGCAGATGA ACAATTTAGT TTCCAACTTA 
AACAAGTTAG AGCAAGTTGG CAGCTCTGAG ATGAACCGCA TCTTCACACG GAGCCAAAAG 
TTAAACAGTG AGGCAATAAT TGACTTTGTG AAGGCTCTTT GCAAGGTTTC TGTGGAGGAA 
TTGAGATCAG CTTCTGATCC ACGGGTCTTT AGCCTTACAA AGATTGTTGA GATTGCGCAC 
TTTAACATGA ATCGCATCAG GCTTGTATGG TCAAGCATCT GGCATGTGCT TTCTGATTTC 
TTTGTAACCA TTGGCTGTTC TGAAAACCTT TCCATTGCAA TTTTTGCAAT GGACTCTTTA 
CGCCAATTAT CAATGAAATT CTTAGACCGA GAAGAGTTGG CAAACTATAA TTTTCAGAAT 
GAGTTTATGA AGCCATTTGT TATTGTTATG CGGAAGAGTA ATGCTGTTGA AATCAGAGAA 
TTGATTATCA GATGTGTCTC GCAAATGGTC CTGTCTCGTG TCAATAATGT TAAATCTGGA 
TGGAAGAGCA TGTTCATGGT TTTCACAGCA GCAGCTTATG ATGACCACAA AAACATTGTA 
CTCTTGGCCT TTGAAATAAT TGAAAAGATT ATTAGAGATT ACTTCCCATA CATTACGGAG 
ACTGAAACAA CCACCTTTAC TGATTGTGTG AATTGCCTGA TAGCATTCAC CAATAGTAGA 
TTCAACAAAG ACATTAGCCT TAATGCAATT GCTTTTCTTC AATTCTGTGC CACAAAACTT 
GCTGAAGGAG ATCTTGGATC TTCATCAAGG AACAAAGACA AGGAAGTCTC TGTAAAAATT 
TCATCCCCTT CACCTCGGAC TGGAAAAGAT GGAAAACAAG AGAATGGAGA AATCAAAGAC 
AAGGAAGATC ATCTTTATTT CTGGTTCCCT CTGTTGGCTG GTTTATCAGA ACTTAGCTTT 
GACCCTAGGC CTGAAGTCAG GAAGAGTGCC TTGCAAGTTC TGTTTGAAAC TTTACGCAAT 
CATGGTCATC TTTTTTCTCT ACCTTTATGG GAAAGAGTCT TTGAGTCGGT CTTATTTCCT 
ATATTTGACT ATGTGCGGCA TGCTATTGAT CCTCCTGGAG GCAACTCCCC TGAGCAGGGG 
ATTGATGGTG ATATGGGTGA ACTTGATCAA GATGCATGGC TCTATGGGAC ATGTACCTTG 
GCTCTCCAAC TGGTTGTAGA TCTTTTTGTG AAATTCTACA ATACTGTCAA TCCGCTTTTA 
AGAAAGGTGC TATCGCTTCT GGTTAGCTTC ATTAGGCGTC CTCACCAAAG TCTTGCTGGT 
ATTGGTATTG CGGCATTTGT GCGTTTGATG AGCAATGCTG GAGATATGTT TTCAGAGGAG 
AAGTGGCTGG AAGTGGTTTT GTCATTGAAA GATGCTGCCA ATGCAACACT CCCTGATTTC 
TCTTACATTG TAAGTGGAGA ATCATCGGTA ATAGCCGATG AACAAAATAA TGGAGAGACT 
GCTGGATCTG ACATGCCCGA AGATGAATCA GAGGGACTGG TGACACACCG TCTTTATGCT 
TCTATATCTG ATGCAAAGTG TCGAGCTGCT GTTCAACTTC TGTTGATTCA GGCGGTGATG 
GAGATCTATA GCATGTACCG GTCTCAACTT TCAGCAAAAT GTGCCTTGGT GCTCTTTGAT 
GCTTTGCATG AGGTGGCATC CCATGCTCAC AGTATCAACA CAAATACAAC ATTACGTTCA 
AAACTACAAG AGTTTGGCTC TATGACCCAA ATGCAAGACC CTCCACTATT ACGCCTGGAG 
AATGAGTCCT ATCAAATTTG CCTCACATTC TTACAAAATC TTATGCTGGA CAGACCTCCA 
CCTTTTGATG AAGCTGAAGT GGAATCCTGC CTTGTCAACC TCTGTGAAGA GGTTTTACAG 
TTTTACGTTG TAACTGCCTG TTCTGGACAG GCATCAGAAA CTTCTACCAG TGGACAATGC 
CTGTGGCTGA TTCCTTTAGG TTCTGGAAAA CGAAGGGAAT TGGCTGCACG TGCACCTCTT 
ATTGTTGCAA CTCTCCAGGC CATCTGTAGT TTGGGAGACT CTTCGTTTGA GAAGAAATTG 
CCTCATTTCT TCCCTCTTCT TTCGAGTTTG ATAAGTTGTG AGCATGGGTC AAATGAGGTC 
CAGGTAGCTC TCAGTGACAT GCTCAGCTCA TCAGTGGGTC CTGTTTTGCT TCGGTCTTGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002301299.2
CDS	88..4170
Translation	MIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRILENVVQPNFQQKIIVLRFLDKLCVDSQILVDIFINYDCDINSSNIFERMVNGLLKTAQGALPGTATTLVPPQEVTMKLEAMKSLVAILKSMGDWMNKQLRIPDPHSAKKSDAAENSPGPGSLPMTNGNGDEPVEGSDSHSETSTEASDVSAIEQRRAYKLEFQEGISLFNRKPKKGIEFLINANKVGNSAEEIAAFLKNASGLNKTLIGDYLGEREDFSLKVMHAYVDSFDFRGLEFDEAIRVFLQGFRLPGEAQKIDRIMEKFAERYCKCNPKVFSSADTAYVLAYSVILLNTDAHNPMVKNKMSADDFIRNNRGIDDGKDLPEEYLRSLFERISKNEIKMKEYDLALQQKQSLNSNRVLGLDSILNIVIRKRGEEKNMETSDDLIRHMQEQFKEKARKSESVYYAATDVVILRFMIEVCWAPMLAAFSVPLDQSDDEVVIALCLEGIRYAIHVTAVMSMKTHRDAFVTSLAKFTSLHSPADIKQKNIDAIKAIVTIADEDGNYLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNNSEKSKQSKSTILPVLKKKGPGRMQHAAASVLRGSYDSAGIGGNAAGAVTSEQMNNLVSNLNKLEQVGSSEMNRIFTRSQKLNSEAIIDFVKALCKVSVEELRSASDPRVFSLTKIVEIAHFNMNRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFMKPFVIVMRKSNAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTAAAYDDHKNIVLLAFEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNSRFNKDISLNAIAFLQFCATKLAEGDLGSSSRNKDKEVSVKISSPSPRTGKDGKQENGEIKDKEDHLYFWFPLLAGLSELSFDPRPEVRKSALQVLFETLRNHGHLFSLPLWERVFESVLFPIFDYVRHAIDPPGGNSPEQGIDGDMGELDQDAWLYGTCTLALQLVVDLFVKFYNTVNPLLRKVLSLLVSFIRRPHQSLAGIGIAAFVRLMSNAGDMFSEEKWLEVVLSLKDAANATLPDFSYIVSGESSVIADEQNNGETAGSDMPEDESEGLVTHRLYASISDAKCRAAVQLLLIQAVMEIYSMYRSQLSAKCALVLFDALHEVASHAHSINTNTTLRSKLQEFGSMTQMQDPPLLRLENESYQICLTFLQNLMLDRPPPFDEAEVESCLVNLCEEVLQFYVVTACSGQASETSTSGQCLWLIPLGSGKRRELAARAPLIVATLQAICSLGDSSFEKKLPHFFPLLSSLISCEHGSNEVQVALSDMLSSSVGPVLLRSC

Target ORF information:

RefSeq Version	XM_002301263.2
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa hypothetical protein (POPTR_0002s15020g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002301263.2

ORF Insert Sequence:

ATGATCATTT TCCAGCTTTC TTGCTCCATT TTCATTAGTT TGGTTTCAAG GTTCAGAGCT 
GGACTGAAAG CAGAAATTGG AGTATTTTTT CCTATGATTG TTCTCAGGAT TCTTGAGAAT 
GTTGTTCAAC CTAATTTTCA GCAGAAGATT ATAGTACTTC GGTTTCTAGA TAAGCTCTGT 
GTTGATTCCC AGATCTTGGT AGACATTTTT ATTAATTATG ACTGTGATAT CAACTCATCA 
AACATATTTG AGAGAATGGT CAATGGACTT CTTAAAACTG CTCAAGGTGC CCTGCCTGGT 
ACTGCCACTA CACTGGTGCC ACCTCAGGAG GTGACCATGA AACTTGAAGC TATGAAGTCC 
TTAGTGGCTA TTCTGAAATC AATGGGAGAC TGGATGAATA AACAATTGCG CATTCCCGAT 
CCTCATTCTG CCAAGAAATC TGATGCAGCC GAAAACAGTC CTGGACCTGG AAGTCTTCCC 
ATGACAAATG GTAATGGGGA TGAGCCTGTT GAAGGATCAG ATTCTCATTC TGAAACCTCA 
ACTGAAGCAT CGGATGTTTC AGCTATTGAG CAACGACGGG CTTACAAGCT GGAATTTCAG 
GAAGGTATAT CTCTTTTCAA TCGGAAGCCT AAGAAAGGAA TTGAATTTCT AATCAATGCA 
AATAAAGTGG GGAATTCAGC AGAAGAGATA GCAGCCTTTC TTAAAAACGC ATCTGGCTTG 
AACAAGACTC TGATTGGCGA TTACCTTGGG GAAAGAGAAG ATTTTTCACT GAAAGTAATG 
CATGCTTATG TAGATTCTTT TGACTTTCGC GGCCTGGAGT TCGATGAGGC TATCAGAGTC 
TTTCTACAGG GCTTTAGGTT ACCAGGTGAG GCACAAAAGA TTGATCGAAT TATGGAGAAG 
TTTGCTGAAC GTTACTGCAA ATGTAACCCA AAGGTTTTTA GCAGTGCTGA CACAGCCTAT 
GTCCTTGCTT ACTCCGTGAT ACTGCTCAAC ACTGATGCTC ATAATCCCAT GGTGAAGAAT 
AAGATGTCAG CTGATGACTT CATAAGAAAC AACCGTGGCA TTGATGATGG GAAAGATCTA 
CCTGAGGAGT ACTTGAGATC TTTGTTTGAA AGAATATCAA AAAATGAGAT CAAAATGAAA 
GAATATGATT TAGCTCTCCA ACAAAAGCAG TCTTTGAATT CAAACAGAGT TTTAGGCTTG 
GATAGTATCC TGAACATTGT GATCCGCAAG CGGGGTGAAG AAAAGAATAT GGAAACAAGT 
GATGATCTTA TCAGGCACAT GCAGGAACAA TTTAAAGAAA AAGCTCGAAA ATCTGAGTCA 
GTTTATTATG CTGCAACAGA TGTGGTGATT CTTAGATTCA TGATTGAGGT ATGCTGGGCT 
CCTATGCTGG CAGCCTTCAG TGTGCCTCTT GACCAAAGTG ATGATGAAGT GGTGATAGCT 
TTGTGTCTTG AAGGTATCCG CTATGCAATC CATGTTACTG CTGTGATGTC CATGAAGACT 
CATAGAGATG CTTTTGTGAC TTCATTAGCA AAGTTTACAT CGCTCCACTC CCCTGCTGAT 
ATCAAGCAGA AAAATATTGA TGCTATCAAG GCAATAGTTA CAATTGCAGA TGAAGATGGG 
AATTATTTGC AAGAAGCTTG GGAACATATT TTGACATGTG TCTCACGCTT TGAACATTTG 
CATCTCCTGG GAGAGGGTGC TCCTCCAGAT GCCACTTTTT TTGCTTTTCC CCAAAATAAC 
TCTGAAAAGT CAAAACAAAG CAAGTCAACC ATCCTCCCTG TTCTGAAAAA GAAGGGACCC 
GGGAGGATGC AGCATGCAGC TGCTAGTGTG CTGAGAGGTT CATATGATAG TGCTGGTATT 
GGTGGAAACG CTGCTGGGGC TGTCACAAGT GAGCAGATGA ACAATTTAGT TTCCAACTTA 
AACAAGTTAG AGCAAGTTGG CAGCTCTGAG ATGAACCGCA TCTTCACACG GAGCCAAAAG 
TTAAACAGTG AGGCAATAAT TGACTTTGTG AAGGCTCTTT GCAAGGTTTC TGTGGAGGAA 
TTGAGATCAG CTTCTGATCC ACGGGTCTTT AGCCTTACAA AGATTGTTGA GATTGCGCAC 
TTTAACATGA ATCGCATCAG GCTTGTATGG TCAAGCATCT GGCATGTGCT TTCTGATTTC 
TTTGTAACCA TTGGCTGTTC TGAAAACCTT TCCATTGCAA TTTTTGCAAT GGACTCTTTA 
CGCCAATTAT CAATGAAATT CTTAGACCGA GAAGAGTTGG CAAACTATAA TTTTCAGAAT 
GAGTTTATGA AGCCATTTGT TATTGTTATG CGGAAGAGTA ATGCTGTTGA AATCAGAGAA 
TTGATTATCA GATGTGTCTC GCAAATGGTC CTGTCTCGTG TCAATAATGT TAAATCTGGA 
TGGAAGAGCA TGTTCATGGT TTTCACAGCA GCAGCTTATG ATGACCACAA AAACATTGTA 
CTCTTGGCCT TTGAAATAAT TGAAAAGATT ATTAGAGATT ACTTCCCATA CATTACGGAG 
ACTGAAACAA CCACCTTTAC TGATTGTGTG AATTGCCTGA TAGCATTCAC CAATAGTAGA 
TTCAACAAAG ACATTAGCCT TAATGCAATT GCTTTTCTTC AATTCTGTGC CACAAAACTT 
GCTGAAGGAG ATCTTGGATC TTCATCAAGG AACAAAGACA AGGAAGTCTC TGTAAAAATT 
TCATCCCCTT CACCTCGGAC TGGAAAAGAT GGAAAACAAG AGAATGGAGA AATCAAAGAC 
AAGGAAGATC ATCTTTATTT CTGGTTCCCT CTGTTGGCTG GTTTATCAGA ACTTAGCTTT 
GACCCTAGGC CTGAAGTCAG GAAGAGTGCC TTGCAAGTTC TGTTTGAAAC TTTACGCAAT 
CATGGTCATC TTTTTTCTCT ACCTTTATGG GAAAGAGTCT TTGAGTCGGT CTTATTTCCT 
ATATTTGACT ATGTGCGGCA TGCTATTGAT CCTCCTGGAG GCAACTCCCC TGAGCAGGGG 
ATTGATGGTG ATATGGGTGA ACTTGATCAA GATGCATGGC TCTATGGGAC ATGTACCTTG 
GCTCTCCAAC TGGTTGTAGA TCTTTTTGTG AAATTCTACA ATACTGTCAA TCCGCTTTTA 
AGAAAGGTGC TATCGCTTCT GGTTAGCTTC ATTAGGCGTC CTCACCAAAG TCTTGCTGGT 
ATTGGTATTG CGGCATTTGT GCGTTTGATG AGCAATGCTG GAGATATGTT TTCAGAGGAG 
AAGTGGCTGG AAGTGGTTTT GTCATTGAAA GATGCTGCCA ATGCAACACT CCCTGATTTC 
TCTTACATTG TAAGTGGAGA ATCATCGGTA ATAGCCGATG AACAAAATAA TGGAGAGACT 
GCTGGATCTG ACATGCCCGA AGATGAATCA GAGGGACTGG TGACACACCG TCTTTATGCT 
TCTATATCTG ATGCAAAGTG TCGAGCTGCT GTTCAACTTC TGTTGATTCA GGCGGTGATG 
GAGATCTATA GCATGTACCG GTCTCAACTT TCAGCAAAAT GTGCCTTGGT GCTCTTTGAT 
GCTTTGCATG AGGTGGCATC CCATGCTCAC AGTATCAACA CAAATACAAC ATTACGTTCA 
AAACTACAAG AGTTTGGCTC TATGACCCAA ATGCAAGACC CTCCACTATT ACGCCTGGAG 
AATGAGTCCT ATCAAATTTG CCTCACATTC TTACAAAATC TTATGCTGGA CAGACCTCCA 
CCTTTTGATG AAGCTGAAGT GGAATCCTGC CTTGTCAACC TCTGTGAAGA GGTTTTACAG 
TTTTACGTTG TAACTGCCTG TTCTGGACAG GCATCAGAAA CTTCTACCAG TGGACAATGC 
CTGTGGCTGA TTCCTTTAGG TTCTGGAAAA CGAAGGGAAT TGGCTGCACG TGCACCTCTT 
ATTGTTGCAA CTCTCCAGGC CATCTGTAGT TTGGGAGACT CTTCGTTTGA GAAGAAATTG 
CCTCATTTCT TCCCTCTTCT TTCGAGTTTG ATAAGTTGTG AGCATGGGTC AAATGAGGTC 
CAGGTAGCTC TCAGTGACAT GCTCAGCTCA TCAGTGGGTC CTGTTTTGCT TCGGTCTTGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0002s15020g ( XM_024595681.1 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_024451449.1 Populus trichocarpa brefeldin A-inhibited guanine nucleotide-exchange protein 2 (LOC7494008), transcript variant X3, mRNA.

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CloneID	OPa424978
Clone ID Related Accession (Same CDS sequence)	XM_024595681.1 , XM_024595680.1
Accession Version	XM_024595681.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037286.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCATTT TCCAGCTTTC TTGCTCCATT TTCATTAGTT TGGTTTCAAG GTTCAGAGCT 
GGACTGAAAG CAGAAATTGG AGTATTTTTT CCTATGATTG TTCTCAGGAT TCTTGAGAAT 
GTTGTTCAAC CTAATTTTCA GCAGAAGATT ATAGTACTTC GGTTTCTAGA TAAGCTCTGT 
GTTGATTCCC AGATCTTGGT AGACATTTTT ATTAATTATG ACTGTGATAT CAACTCATCA 
AACATATTTG AGAGAATGGT CAATGGACTT CTTAAAACTG CTCAAGGTGC CCTGCCTGGT 
ACTGCCACTA CACTGGTGCC ACCTCAGGAG GTGACCATGA AACTTGAAGC TATGAAGTCC 
TTAGTGGCTA TTCTGAAATC AATGGGAGAC TGGATGAATA AACAATTGCG CATTCCCGAT 
CCTCATTCTG CCAAGAAATC TGATGCAGCC GAAAACAGTC CTGGACCTGG AAGTCTTCCC 
ATGACAAATG GTAATGGGGA TGAGCCTGTT GAAGGATCAG ATTCTCATTC TGAAACCTCA 
ACTGAAGCAT CGGATGTTTC AGCTATTGAG CAACGACGGG CTTACAAGCT GGAATTTCAG 
GAAGGTATAT CTCTTTTCAA TCGGAAGCCT AAGAAAGGAA TTGAATTTCT AATCAATGCA 
AATAAAGTGG GGAATTCAGC AGAAGAGATA GCAGCCTTTC TTAAAAACGC ATCTGGCTTG 
AACAAGACTC TGATTGGCGA TTACCTTGGG GAAAGAGAAG ATTTTTCACT GAAAGTAATG 
CATGCTTATG TAGATTCTTT TGACTTTCGC GGCCTGGAGT TCGATGAGGC TATCAGAGTC 
TTTCTACAGG GCTTTAGGTT ACCAGGTGAG GCACAAAAGA TTGATCGAAT TATGGAGAAG 
TTTGCTGAAC GTTACTGCAA ATGTAACCCA AAGGTTTTTA GCAGTGCTGA CACAGCCTAT 
GTCCTTGCTT ACTCCGTGAT ACTGCTCAAC ACTGATGCTC ATAATCCCAT GGTGAAGAAT 
AAGATGTCAG CTGATGACTT CATAAGAAAC AACCGTGGCA TTGATGATGG GAAAGATCTA 
CCTGAGGAGT ACTTGAGATC TTTGTTTGAA AGAATATCAA AAAATGAGAT CAAAATGAAA 
GAATATGATT TAGCTCTCCA ACAAAAGCAG TCTTTGAATT CAAACAGAGT TTTAGGCTTG 
GATAGTATCC TGAACATTGT GATCCGCAAG CGGGGTGAAG AAAAGAATAT GGAAACAAGT 
GATGATCTTA TCAGGCACAT GCAGGAACAA TTTAAAGAAA AAGCTCGAAA ATCTGAGTCA 
GTTTATTATG CTGCAACAGA TGTGGTGATT CTTAGATTCA TGATTGAGGT ATGCTGGGCT 
CCTATGCTGG CAGCCTTCAG TGTGCCTCTT GACCAAAGTG ATGATGAAGT GGTGATAGCT 
TTGTGTCTTG AAGGTATCCG CTATGCAATC CATGTTACTG CTGTGATGTC CATGAAGACT 
CATAGAGATG CTTTTGTGAC TTCATTAGCA AAGTTTACAT CGCTCCACTC CCCTGCTGAT 
ATAAAGCAGA AAAATATTGA TGCTATCAAG GCAATAGTTA CAATTGCAGA TGAAGATGGG 
AATTATTTGC AAGAAGCTTG GGAACATATT TTGACATGTG TCTCACGCTT TGAACATTTG 
CATCTCCTGG GAGAGGGTGC TCCTCCAGAT GCCACTTTTT TTGCTTTTCC CCAAAATAAC 
TCTGAAAAGT CAAAACAAAG CAAGTCAACC ATCCTCCCTG TTCTGAAAAA GAAGGGACCC 
GGGAGGATGC AGCATGCAGC TGCTAGTGTG CTGAGAGGTT CATATGATAG TGCTGGTATT 
GGTGGAAACG CTGCTGGGGC TGTCACAAGT GAGCAGATGA ACAATTTAGT TTCCAACTTA 
AACAAGTTAG AGCAAGTTGG CAGCTCTGAG ATGAACCGCA TCTTCACACG GAGCCAAAAG 
TTAAACAGTG AGGCAATAAT TGACTTTGTG AAGGCTCTTT GCAAGGTTTC TGTGGAGGAA 
TTGAGATCAG CTTCTGATCC ACGGGTCTTT AGCCTTACAA AGATTGTTGA GATTGCGCAC 
TTTAACATGA ATCGCATCAG GCTTGTATGG TCAAGCATCT GGCATGTGCT TTCTGATTTC 
TTTGTAACCA TTGGCTGTTC TGAAAACCTT TCCATTGCAA TTTTTGCAAT GGACTCTTTA 
CGCCAATTAT CAATGAAATT CTTAGACCGA GAAGAGTTGG CAAACTATAA TTTTCAGAAT 
GAGTTTATGA AGCCATTTGT TATTGTTATG CGGAAGAGTA ATGCTGTTGA AATCAGAGAA 
TTGATTATCA GATGTGTCTC GCAAATGGTC CTGTCTCGTG TCAATAATGT TAAATCTGGA 
TGGAAGAGCA TGTTCATGGT TTTCACAGCA GCAGCTTATG ATGACCACAA AAACATTGTA 
CTCTTGGCCT TTGAAATAAT TGAAAAGATT ATTAGAGATT ACTTCCCATA CATTACGGAG 
ACTGAAACAA CCACCTTTAC TGATTGTGTG AATTGCCTGA TAGCATTCAC CAATAGTAGA 
TTCAACAAAG ACATTAGCCT TAATGCAATT GCTTTTCTTC AATTCTGTGC CACAAAACTT 
GCTGAAGGAG ATCTTGGATC TTCATCAAGG AACAAAGACA AGGAAGTCTC TGTAAAAATT 
TCATCCCCTT CACCTCGGAC CGGAAAAGAT GGAAAACAAG AGAATGGAGA AATCAAAGAC 
AAGGAAGATC ATCTTTATTT CTGGTTCCCT CTGTTGGCTG GTTTATCAGA ACTTAGCTTT 
GACCCTAGGC CTGAAGTCAG GAAGAGTGCC TTGCAAGTTC TGTTTGAAAC TTTACGCAAT 
CATGGTCATC TTTTTTCTCT ACCTTTATGG GAAAGAGTCT TTGAGTCGGT CTTATTTCCT 
ATATTTGACT ATGTGCGGCA TGCTATTGAT CCTCCTGGAG GCAACTCCCC TGAGCAGGGG 
ATTGATGGTG ATATGGGTGA ACTTGATCAA GATGCATGGC TCTATGGGAC ATGTACCTTG 
GCTCTCCAAC TGGTTGTAGA TCTTTTTGTG AAATTCTACA ATACTGTCAA TCCGCTTTTA 
AGAAAGGTGC TATCGCTTCT GGTTAGCTTC ATTAGGCGTC CTCACCAAAG TCTTGCTGGT 
ATTGGTATTG CGGCATTTGT GCGTTTGATG AGCAATGCTG GAGATATGTT TTCAGAGGAG 
AAGTGGCTGG AAGTGGTTTT GTCATTGAAA GATGCTGCCA ATGCAACACT CCCTGATTTC 
TCTTACATTG TAAGTGGAGA ATCATCGGTA ATAGCCGATG AACAAAATAA TGGAGAGACT 
GCTGGATCTG ACATGCCCGA AGATGAATCA GAGGGACTGG TGACACACCA TCTTTATGCT 
TCTATATCTG ATGCAAAGTG TCGAGCTGCT GTTCAACTTC TGTTGATTCA GGCGGTGATG 
GAGATCTATA GCATGTACCG GTCTCAACTT TCAGCAAAAT GTGCCTTGGT GCTCTTTGAT 
GCTTTGCATG AGGTGGCATC CCATGCTCAC AGTATCAACA CAAATACAAC ATTACGTTCA 
AAACTACAAG AGTTTGGCTC TATGACCCAA ATGCAAGACC CTCCACTATT ACGCCTGGAG 
AATGAGTCCT ATCAAATTTG CCTCACATTC TTACAAAATC TTATGATGGA CAGACCTCCA 
CCTTTTGATG AAGCTGAAGT GGAATCCTGC CTTGTCAACC TCTGTGAAGA GGTTTTACAG 
TTTTACGTTG TAACTGCCTG TTCTGGACAG GCATCAGAAA CTTCTACCAG TGGACAATGC 
CTGTGGCTGA TTCCTTTAGG TTCTGGAAAA CGAAGGGAAT TGGCTGCACG TGCACCTCTT 
ATTGTTGCAA CTCTCCAGGC CATCTGTAGT TTGGGAGACT CTTCGTTTGA GAAGAAATTG 
CCTCATTTCT TCCCTCTTCT TTCGAGTTTG ATAAGTTGTG AGCATGGGTC AAATGAGGTC 
CAGGTAGCTC TCAGTGACAT GCTCAGCTCA TCAGTGGGTC CTGTTTTGCT TCGGTCTTGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024451449.1
CDS	337..4419
Translation	MIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRILENVVQPNFQQKIIVLRFLDKLCVDSQILVDIFINYDCDINSSNIFERMVNGLLKTAQGALPGTATTLVPPQEVTMKLEAMKSLVAILKSMGDWMNKQLRIPDPHSAKKSDAAENSPGPGSLPMTNGNGDEPVEGSDSHSETSTEASDVSAIEQRRAYKLEFQEGISLFNRKPKKGIEFLINANKVGNSAEEIAAFLKNASGLNKTLIGDYLGEREDFSLKVMHAYVDSFDFRGLEFDEAIRVFLQGFRLPGEAQKIDRIMEKFAERYCKCNPKVFSSADTAYVLAYSVILLNTDAHNPMVKNKMSADDFIRNNRGIDDGKDLPEEYLRSLFERISKNEIKMKEYDLALQQKQSLNSNRVLGLDSILNIVIRKRGEEKNMETSDDLIRHMQEQFKEKARKSESVYYAATDVVILRFMIEVCWAPMLAAFSVPLDQSDDEVVIALCLEGIRYAIHVTAVMSMKTHRDAFVTSLAKFTSLHSPADIKQKNIDAIKAIVTIADEDGNYLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNNSEKSKQSKSTILPVLKKKGPGRMQHAAASVLRGSYDSAGIGGNAAGAVTSEQMNNLVSNLNKLEQVGSSEMNRIFTRSQKLNSEAIIDFVKALCKVSVEELRSASDPRVFSLTKIVEIAHFNMNRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFMKPFVIVMRKSNAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTAAAYDDHKNIVLLAFEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNSRFNKDISLNAIAFLQFCATKLAEGDLGSSSRNKDKEVSVKISSPSPRTGKDGKQENGEIKDKEDHLYFWFPLLAGLSELSFDPRPEVRKSALQVLFETLRNHGHLFSLPLWERVFESVLFPIFDYVRHAIDPPGGNSPEQGIDGDMGELDQDAWLYGTCTLALQLVVDLFVKFYNTVNPLLRKVLSLLVSFIRRPHQSLAGIGIAAFVRLMSNAGDMFSEEKWLEVVLSLKDAANATLPDFSYIVSGESSVIADEQNNGETAGSDMPEDESEGLVTHHLYASISDAKCRAAVQLLLIQAVMEIYSMYRSQLSAKCALVLFDALHEVASHAHSINTNTTLRSKLQEFGSMTQMQDPPLLRLENESYQICLTFLQNLMMDRPPPFDEAEVESCLVNLCEEVLQFYVVTACSGQASETSTSGQCLWLIPLGSGKRRELAARAPLIVATLQAICSLGDSSFEKKLPHFFPLLSSLISCEHGSNEVQVALSDMLSSSVGPVLLRSC

Target ORF information:

RefSeq Version	XM_024595681.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa brefeldin A-inhibited guanine nucleotide-exchange protein 2 (LOC7494008), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024595681.1

ORF Insert Sequence:

ATGATCATTT TCCAGCTTTC TTGCTCCATT TTCATTAGTT TGGTTTCAAG GTTCAGAGCT 
GGACTGAAAG CAGAAATTGG AGTATTTTTT CCTATGATTG TTCTCAGGAT TCTTGAGAAT 
GTTGTTCAAC CTAATTTTCA GCAGAAGATT ATAGTACTTC GGTTTCTAGA TAAGCTCTGT 
GTTGATTCCC AGATCTTGGT AGACATTTTT ATTAATTATG ACTGTGATAT CAACTCATCA 
AACATATTTG AGAGAATGGT CAATGGACTT CTTAAAACTG CTCAAGGTGC CCTGCCTGGT 
ACTGCCACTA CACTGGTGCC ACCTCAGGAG GTGACCATGA AACTTGAAGC TATGAAGTCC 
TTAGTGGCTA TTCTGAAATC AATGGGAGAC TGGATGAATA AACAATTGCG CATTCCCGAT 
CCTCATTCTG CCAAGAAATC TGATGCAGCC GAAAACAGTC CTGGACCTGG AAGTCTTCCC 
ATGACAAATG GTAATGGGGA TGAGCCTGTT GAAGGATCAG ATTCTCATTC TGAAACCTCA 
ACTGAAGCAT CGGATGTTTC AGCTATTGAG CAACGACGGG CTTACAAGCT GGAATTTCAG 
GAAGGTATAT CTCTTTTCAA TCGGAAGCCT AAGAAAGGAA TTGAATTTCT AATCAATGCA 
AATAAAGTGG GGAATTCAGC AGAAGAGATA GCAGCCTTTC TTAAAAACGC ATCTGGCTTG 
AACAAGACTC TGATTGGCGA TTACCTTGGG GAAAGAGAAG ATTTTTCACT GAAAGTAATG 
CATGCTTATG TAGATTCTTT TGACTTTCGC GGCCTGGAGT TCGATGAGGC TATCAGAGTC 
TTTCTACAGG GCTTTAGGTT ACCAGGTGAG GCACAAAAGA TTGATCGAAT TATGGAGAAG 
TTTGCTGAAC GTTACTGCAA ATGTAACCCA AAGGTTTTTA GCAGTGCTGA CACAGCCTAT 
GTCCTTGCTT ACTCCGTGAT ACTGCTCAAC ACTGATGCTC ATAATCCCAT GGTGAAGAAT 
AAGATGTCAG CTGATGACTT CATAAGAAAC AACCGTGGCA TTGATGATGG GAAAGATCTA 
CCTGAGGAGT ACTTGAGATC TTTGTTTGAA AGAATATCAA AAAATGAGAT CAAAATGAAA 
GAATATGATT TAGCTCTCCA ACAAAAGCAG TCTTTGAATT CAAACAGAGT TTTAGGCTTG 
GATAGTATCC TGAACATTGT GATCCGCAAG CGGGGTGAAG AAAAGAATAT GGAAACAAGT 
GATGATCTTA TCAGGCACAT GCAGGAACAA TTTAAAGAAA AAGCTCGAAA ATCTGAGTCA 
GTTTATTATG CTGCAACAGA TGTGGTGATT CTTAGATTCA TGATTGAGGT ATGCTGGGCT 
CCTATGCTGG CAGCCTTCAG TGTGCCTCTT GACCAAAGTG ATGATGAAGT GGTGATAGCT 
TTGTGTCTTG AAGGTATCCG CTATGCAATC CATGTTACTG CTGTGATGTC CATGAAGACT 
CATAGAGATG CTTTTGTGAC TTCATTAGCA AAGTTTACAT CGCTCCACTC CCCTGCTGAT 
ATAAAGCAGA AAAATATTGA TGCTATCAAG GCAATAGTTA CAATTGCAGA TGAAGATGGG 
AATTATTTGC AAGAAGCTTG GGAACATATT TTGACATGTG TCTCACGCTT TGAACATTTG 
CATCTCCTGG GAGAGGGTGC TCCTCCAGAT GCCACTTTTT TTGCTTTTCC CCAAAATAAC 
TCTGAAAAGT CAAAACAAAG CAAGTCAACC ATCCTCCCTG TTCTGAAAAA GAAGGGACCC 
GGGAGGATGC AGCATGCAGC TGCTAGTGTG CTGAGAGGTT CATATGATAG TGCTGGTATT 
GGTGGAAACG CTGCTGGGGC TGTCACAAGT GAGCAGATGA ACAATTTAGT TTCCAACTTA 
AACAAGTTAG AGCAAGTTGG CAGCTCTGAG ATGAACCGCA TCTTCACACG GAGCCAAAAG 
TTAAACAGTG AGGCAATAAT TGACTTTGTG AAGGCTCTTT GCAAGGTTTC TGTGGAGGAA 
TTGAGATCAG CTTCTGATCC ACGGGTCTTT AGCCTTACAA AGATTGTTGA GATTGCGCAC 
TTTAACATGA ATCGCATCAG GCTTGTATGG TCAAGCATCT GGCATGTGCT TTCTGATTTC 
TTTGTAACCA TTGGCTGTTC TGAAAACCTT TCCATTGCAA TTTTTGCAAT GGACTCTTTA 
CGCCAATTAT CAATGAAATT CTTAGACCGA GAAGAGTTGG CAAACTATAA TTTTCAGAAT 
GAGTTTATGA AGCCATTTGT TATTGTTATG CGGAAGAGTA ATGCTGTTGA AATCAGAGAA 
TTGATTATCA GATGTGTCTC GCAAATGGTC CTGTCTCGTG TCAATAATGT TAAATCTGGA 
TGGAAGAGCA TGTTCATGGT TTTCACAGCA GCAGCTTATG ATGACCACAA AAACATTGTA 
CTCTTGGCCT TTGAAATAAT TGAAAAGATT ATTAGAGATT ACTTCCCATA CATTACGGAG 
ACTGAAACAA CCACCTTTAC TGATTGTGTG AATTGCCTGA TAGCATTCAC CAATAGTAGA 
TTCAACAAAG ACATTAGCCT TAATGCAATT GCTTTTCTTC AATTCTGTGC CACAAAACTT 
GCTGAAGGAG ATCTTGGATC TTCATCAAGG AACAAAGACA AGGAAGTCTC TGTAAAAATT 
TCATCCCCTT CACCTCGGAC CGGAAAAGAT GGAAAACAAG AGAATGGAGA AATCAAAGAC 
AAGGAAGATC ATCTTTATTT CTGGTTCCCT CTGTTGGCTG GTTTATCAGA ACTTAGCTTT 
GACCCTAGGC CTGAAGTCAG GAAGAGTGCC TTGCAAGTTC TGTTTGAAAC TTTACGCAAT 
CATGGTCATC TTTTTTCTCT ACCTTTATGG GAAAGAGTCT TTGAGTCGGT CTTATTTCCT 
ATATTTGACT ATGTGCGGCA TGCTATTGAT CCTCCTGGAG GCAACTCCCC TGAGCAGGGG 
ATTGATGGTG ATATGGGTGA ACTTGATCAA GATGCATGGC TCTATGGGAC ATGTACCTTG 
GCTCTCCAAC TGGTTGTAGA TCTTTTTGTG AAATTCTACA ATACTGTCAA TCCGCTTTTA 
AGAAAGGTGC TATCGCTTCT GGTTAGCTTC ATTAGGCGTC CTCACCAAAG TCTTGCTGGT 
ATTGGTATTG CGGCATTTGT GCGTTTGATG AGCAATGCTG GAGATATGTT TTCAGAGGAG 
AAGTGGCTGG AAGTGGTTTT GTCATTGAAA GATGCTGCCA ATGCAACACT CCCTGATTTC 
TCTTACATTG TAAGTGGAGA ATCATCGGTA ATAGCCGATG AACAAAATAA TGGAGAGACT 
GCTGGATCTG ACATGCCCGA AGATGAATCA GAGGGACTGG TGACACACCA TCTTTATGCT 
TCTATATCTG ATGCAAAGTG TCGAGCTGCT GTTCAACTTC TGTTGATTCA GGCGGTGATG 
GAGATCTATA GCATGTACCG GTCTCAACTT TCAGCAAAAT GTGCCTTGGT GCTCTTTGAT 
GCTTTGCATG AGGTGGCATC CCATGCTCAC AGTATCAACA CAAATACAAC ATTACGTTCA 
AAACTACAAG AGTTTGGCTC TATGACCCAA ATGCAAGACC CTCCACTATT ACGCCTGGAG 
AATGAGTCCT ATCAAATTTG CCTCACATTC TTACAAAATC TTATGATGGA CAGACCTCCA 
CCTTTTGATG AAGCTGAAGT GGAATCCTGC CTTGTCAACC TCTGTGAAGA GGTTTTACAG 
TTTTACGTTG TAACTGCCTG TTCTGGACAG GCATCAGAAA CTTCTACCAG TGGACAATGC 
CTGTGGCTGA TTCCTTTAGG TTCTGGAAAA CGAAGGGAAT TGGCTGCACG TGCACCTCTT 
ATTGTTGCAA CTCTCCAGGC CATCTGTAGT TTGGGAGACT CTTCGTTTGA GAAGAAATTG 
CCTCATTTCT TCCCTCTTCT TTCGAGTTTG ATAAGTTGTG AGCATGGGTC AAATGAGGTC 
CAGGTAGCTC TCAGTGACAT GCTCAGCTCA TCAGTGGGTC CTGTTTTGCT TCGGTCTTGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0002s15020g ( XM_024595680.1 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_024451448.1 Populus trichocarpa brefeldin A-inhibited guanine nucleotide-exchange protein 2 (LOC7494008), transcript variant X2, mRNA.

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CloneID	OPa424978
Clone ID Related Accession (Same CDS sequence)	XM_024595681.1 , XM_024595680.1
Accession Version	XM_024595680.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037286.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCATTT TCCAGCTTTC TTGCTCCATT TTCATTAGTT TGGTTTCAAG GTTCAGAGCT 
GGACTGAAAG CAGAAATTGG AGTATTTTTT CCTATGATTG TTCTCAGGAT TCTTGAGAAT 
GTTGTTCAAC CTAATTTTCA GCAGAAGATT ATAGTACTTC GGTTTCTAGA TAAGCTCTGT 
GTTGATTCCC AGATCTTGGT AGACATTTTT ATTAATTATG ACTGTGATAT CAACTCATCA 
AACATATTTG AGAGAATGGT CAATGGACTT CTTAAAACTG CTCAAGGTGC CCTGCCTGGT 
ACTGCCACTA CACTGGTGCC ACCTCAGGAG GTGACCATGA AACTTGAAGC TATGAAGTCC 
TTAGTGGCTA TTCTGAAATC AATGGGAGAC TGGATGAATA AACAATTGCG CATTCCCGAT 
CCTCATTCTG CCAAGAAATC TGATGCAGCC GAAAACAGTC CTGGACCTGG AAGTCTTCCC 
ATGACAAATG GTAATGGGGA TGAGCCTGTT GAAGGATCAG ATTCTCATTC TGAAACCTCA 
ACTGAAGCAT CGGATGTTTC AGCTATTGAG CAACGACGGG CTTACAAGCT GGAATTTCAG 
GAAGGTATAT CTCTTTTCAA TCGGAAGCCT AAGAAAGGAA TTGAATTTCT AATCAATGCA 
AATAAAGTGG GGAATTCAGC AGAAGAGATA GCAGCCTTTC TTAAAAACGC ATCTGGCTTG 
AACAAGACTC TGATTGGCGA TTACCTTGGG GAAAGAGAAG ATTTTTCACT GAAAGTAATG 
CATGCTTATG TAGATTCTTT TGACTTTCGC GGCCTGGAGT TCGATGAGGC TATCAGAGTC 
TTTCTACAGG GCTTTAGGTT ACCAGGTGAG GCACAAAAGA TTGATCGAAT TATGGAGAAG 
TTTGCTGAAC GTTACTGCAA ATGTAACCCA AAGGTTTTTA GCAGTGCTGA CACAGCCTAT 
GTCCTTGCTT ACTCCGTGAT ACTGCTCAAC ACTGATGCTC ATAATCCCAT GGTGAAGAAT 
AAGATGTCAG CTGATGACTT CATAAGAAAC AACCGTGGCA TTGATGATGG GAAAGATCTA 
CCTGAGGAGT ACTTGAGATC TTTGTTTGAA AGAATATCAA AAAATGAGAT CAAAATGAAA 
GAATATGATT TAGCTCTCCA ACAAAAGCAG TCTTTGAATT CAAACAGAGT TTTAGGCTTG 
GATAGTATCC TGAACATTGT GATCCGCAAG CGGGGTGAAG AAAAGAATAT GGAAACAAGT 
GATGATCTTA TCAGGCACAT GCAGGAACAA TTTAAAGAAA AAGCTCGAAA ATCTGAGTCA 
GTTTATTATG CTGCAACAGA TGTGGTGATT CTTAGATTCA TGATTGAGGT ATGCTGGGCT 
CCTATGCTGG CAGCCTTCAG TGTGCCTCTT GACCAAAGTG ATGATGAAGT GGTGATAGCT 
TTGTGTCTTG AAGGTATCCG CTATGCAATC CATGTTACTG CTGTGATGTC CATGAAGACT 
CATAGAGATG CTTTTGTGAC TTCATTAGCA AAGTTTACAT CGCTCCACTC CCCTGCTGAT 
ATAAAGCAGA AAAATATTGA TGCTATCAAG GCAATAGTTA CAATTGCAGA TGAAGATGGG 
AATTATTTGC AAGAAGCTTG GGAACATATT TTGACATGTG TCTCACGCTT TGAACATTTG 
CATCTCCTGG GAGAGGGTGC TCCTCCAGAT GCCACTTTTT TTGCTTTTCC CCAAAATAAC 
TCTGAAAAGT CAAAACAAAG CAAGTCAACC ATCCTCCCTG TTCTGAAAAA GAAGGGACCC 
GGGAGGATGC AGCATGCAGC TGCTAGTGTG CTGAGAGGTT CATATGATAG TGCTGGTATT 
GGTGGAAACG CTGCTGGGGC TGTCACAAGT GAGCAGATGA ACAATTTAGT TTCCAACTTA 
AACAAGTTAG AGCAAGTTGG CAGCTCTGAG ATGAACCGCA TCTTCACACG GAGCCAAAAG 
TTAAACAGTG AGGCAATAAT TGACTTTGTG AAGGCTCTTT GCAAGGTTTC TGTGGAGGAA 
TTGAGATCAG CTTCTGATCC ACGGGTCTTT AGCCTTACAA AGATTGTTGA GATTGCGCAC 
TTTAACATGA ATCGCATCAG GCTTGTATGG TCAAGCATCT GGCATGTGCT TTCTGATTTC 
TTTGTAACCA TTGGCTGTTC TGAAAACCTT TCCATTGCAA TTTTTGCAAT GGACTCTTTA 
CGCCAATTAT CAATGAAATT CTTAGACCGA GAAGAGTTGG CAAACTATAA TTTTCAGAAT 
GAGTTTATGA AGCCATTTGT TATTGTTATG CGGAAGAGTA ATGCTGTTGA AATCAGAGAA 
TTGATTATCA GATGTGTCTC GCAAATGGTC CTGTCTCGTG TCAATAATGT TAAATCTGGA 
TGGAAGAGCA TGTTCATGGT TTTCACAGCA GCAGCTTATG ATGACCACAA AAACATTGTA 
CTCTTGGCCT TTGAAATAAT TGAAAAGATT ATTAGAGATT ACTTCCCATA CATTACGGAG 
ACTGAAACAA CCACCTTTAC TGATTGTGTG AATTGCCTGA TAGCATTCAC CAATAGTAGA 
TTCAACAAAG ACATTAGCCT TAATGCAATT GCTTTTCTTC AATTCTGTGC CACAAAACTT 
GCTGAAGGAG ATCTTGGATC TTCATCAAGG AACAAAGACA AGGAAGTCTC TGTAAAAATT 
TCATCCCCTT CACCTCGGAC CGGAAAAGAT GGAAAACAAG AGAATGGAGA AATCAAAGAC 
AAGGAAGATC ATCTTTATTT CTGGTTCCCT CTGTTGGCTG GTTTATCAGA ACTTAGCTTT 
GACCCTAGGC CTGAAGTCAG GAAGAGTGCC TTGCAAGTTC TGTTTGAAAC TTTACGCAAT 
CATGGTCATC TTTTTTCTCT ACCTTTATGG GAAAGAGTCT TTGAGTCGGT CTTATTTCCT 
ATATTTGACT ATGTGCGGCA TGCTATTGAT CCTCCTGGAG GCAACTCCCC TGAGCAGGGG 
ATTGATGGTG ATATGGGTGA ACTTGATCAA GATGCATGGC TCTATGGGAC ATGTACCTTG 
GCTCTCCAAC TGGTTGTAGA TCTTTTTGTG AAATTCTACA ATACTGTCAA TCCGCTTTTA 
AGAAAGGTGC TATCGCTTCT GGTTAGCTTC ATTAGGCGTC CTCACCAAAG TCTTGCTGGT 
ATTGGTATTG CGGCATTTGT GCGTTTGATG AGCAATGCTG GAGATATGTT TTCAGAGGAG 
AAGTGGCTGG AAGTGGTTTT GTCATTGAAA GATGCTGCCA ATGCAACACT CCCTGATTTC 
TCTTACATTG TAAGTGGAGA ATCATCGGTA ATAGCCGATG AACAAAATAA TGGAGAGACT 
GCTGGATCTG ACATGCCCGA AGATGAATCA GAGGGACTGG TGACACACCA TCTTTATGCT 
TCTATATCTG ATGCAAAGTG TCGAGCTGCT GTTCAACTTC TGTTGATTCA GGCGGTGATG 
GAGATCTATA GCATGTACCG GTCTCAACTT TCAGCAAAAT GTGCCTTGGT GCTCTTTGAT 
GCTTTGCATG AGGTGGCATC CCATGCTCAC AGTATCAACA CAAATACAAC ATTACGTTCA 
AAACTACAAG AGTTTGGCTC TATGACCCAA ATGCAAGACC CTCCACTATT ACGCCTGGAG 
AATGAGTCCT ATCAAATTTG CCTCACATTC TTACAAAATC TTATGATGGA CAGACCTCCA 
CCTTTTGATG AAGCTGAAGT GGAATCCTGC CTTGTCAACC TCTGTGAAGA GGTTTTACAG 
TTTTACGTTG TAACTGCCTG TTCTGGACAG GCATCAGAAA CTTCTACCAG TGGACAATGC 
CTGTGGCTGA TTCCTTTAGG TTCTGGAAAA CGAAGGGAAT TGGCTGCACG TGCACCTCTT 
ATTGTTGCAA CTCTCCAGGC CATCTGTAGT TTGGGAGACT CTTCGTTTGA GAAGAAATTG 
CCTCATTTCT TCCCTCTTCT TTCGAGTTTG ATAAGTTGTG AGCATGGGTC AAATGAGGTC 
CAGGTAGCTC TCAGTGACAT GCTCAGCTCA TCAGTGGGTC CTGTTTTGCT TCGGTCTTGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024451448.1
CDS	356..4438
Translation	MIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRILENVVQPNFQQKIIVLRFLDKLCVDSQILVDIFINYDCDINSSNIFERMVNGLLKTAQGALPGTATTLVPPQEVTMKLEAMKSLVAILKSMGDWMNKQLRIPDPHSAKKSDAAENSPGPGSLPMTNGNGDEPVEGSDSHSETSTEASDVSAIEQRRAYKLEFQEGISLFNRKPKKGIEFLINANKVGNSAEEIAAFLKNASGLNKTLIGDYLGEREDFSLKVMHAYVDSFDFRGLEFDEAIRVFLQGFRLPGEAQKIDRIMEKFAERYCKCNPKVFSSADTAYVLAYSVILLNTDAHNPMVKNKMSADDFIRNNRGIDDGKDLPEEYLRSLFERISKNEIKMKEYDLALQQKQSLNSNRVLGLDSILNIVIRKRGEEKNMETSDDLIRHMQEQFKEKARKSESVYYAATDVVILRFMIEVCWAPMLAAFSVPLDQSDDEVVIALCLEGIRYAIHVTAVMSMKTHRDAFVTSLAKFTSLHSPADIKQKNIDAIKAIVTIADEDGNYLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNNSEKSKQSKSTILPVLKKKGPGRMQHAAASVLRGSYDSAGIGGNAAGAVTSEQMNNLVSNLNKLEQVGSSEMNRIFTRSQKLNSEAIIDFVKALCKVSVEELRSASDPRVFSLTKIVEIAHFNMNRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFMKPFVIVMRKSNAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTAAAYDDHKNIVLLAFEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNSRFNKDISLNAIAFLQFCATKLAEGDLGSSSRNKDKEVSVKISSPSPRTGKDGKQENGEIKDKEDHLYFWFPLLAGLSELSFDPRPEVRKSALQVLFETLRNHGHLFSLPLWERVFESVLFPIFDYVRHAIDPPGGNSPEQGIDGDMGELDQDAWLYGTCTLALQLVVDLFVKFYNTVNPLLRKVLSLLVSFIRRPHQSLAGIGIAAFVRLMSNAGDMFSEEKWLEVVLSLKDAANATLPDFSYIVSGESSVIADEQNNGETAGSDMPEDESEGLVTHHLYASISDAKCRAAVQLLLIQAVMEIYSMYRSQLSAKCALVLFDALHEVASHAHSINTNTTLRSKLQEFGSMTQMQDPPLLRLENESYQICLTFLQNLMMDRPPPFDEAEVESCLVNLCEEVLQFYVVTACSGQASETSTSGQCLWLIPLGSGKRRELAARAPLIVATLQAICSLGDSSFEKKLPHFFPLLSSLISCEHGSNEVQVALSDMLSSSVGPVLLRSC

Target ORF information:

RefSeq Version	XM_024595680.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa brefeldin A-inhibited guanine nucleotide-exchange protein 2 (LOC7494008), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024595680.1

ORF Insert Sequence:

ATGATCATTT TCCAGCTTTC TTGCTCCATT TTCATTAGTT TGGTTTCAAG GTTCAGAGCT 
GGACTGAAAG CAGAAATTGG AGTATTTTTT CCTATGATTG TTCTCAGGAT TCTTGAGAAT 
GTTGTTCAAC CTAATTTTCA GCAGAAGATT ATAGTACTTC GGTTTCTAGA TAAGCTCTGT 
GTTGATTCCC AGATCTTGGT AGACATTTTT ATTAATTATG ACTGTGATAT CAACTCATCA 
AACATATTTG AGAGAATGGT CAATGGACTT CTTAAAACTG CTCAAGGTGC CCTGCCTGGT 
ACTGCCACTA CACTGGTGCC ACCTCAGGAG GTGACCATGA AACTTGAAGC TATGAAGTCC 
TTAGTGGCTA TTCTGAAATC AATGGGAGAC TGGATGAATA AACAATTGCG CATTCCCGAT 
CCTCATTCTG CCAAGAAATC TGATGCAGCC GAAAACAGTC CTGGACCTGG AAGTCTTCCC 
ATGACAAATG GTAATGGGGA TGAGCCTGTT GAAGGATCAG ATTCTCATTC TGAAACCTCA 
ACTGAAGCAT CGGATGTTTC AGCTATTGAG CAACGACGGG CTTACAAGCT GGAATTTCAG 
GAAGGTATAT CTCTTTTCAA TCGGAAGCCT AAGAAAGGAA TTGAATTTCT AATCAATGCA 
AATAAAGTGG GGAATTCAGC AGAAGAGATA GCAGCCTTTC TTAAAAACGC ATCTGGCTTG 
AACAAGACTC TGATTGGCGA TTACCTTGGG GAAAGAGAAG ATTTTTCACT GAAAGTAATG 
CATGCTTATG TAGATTCTTT TGACTTTCGC GGCCTGGAGT TCGATGAGGC TATCAGAGTC 
TTTCTACAGG GCTTTAGGTT ACCAGGTGAG GCACAAAAGA TTGATCGAAT TATGGAGAAG 
TTTGCTGAAC GTTACTGCAA ATGTAACCCA AAGGTTTTTA GCAGTGCTGA CACAGCCTAT 
GTCCTTGCTT ACTCCGTGAT ACTGCTCAAC ACTGATGCTC ATAATCCCAT GGTGAAGAAT 
AAGATGTCAG CTGATGACTT CATAAGAAAC AACCGTGGCA TTGATGATGG GAAAGATCTA 
CCTGAGGAGT ACTTGAGATC TTTGTTTGAA AGAATATCAA AAAATGAGAT CAAAATGAAA 
GAATATGATT TAGCTCTCCA ACAAAAGCAG TCTTTGAATT CAAACAGAGT TTTAGGCTTG 
GATAGTATCC TGAACATTGT GATCCGCAAG CGGGGTGAAG AAAAGAATAT GGAAACAAGT 
GATGATCTTA TCAGGCACAT GCAGGAACAA TTTAAAGAAA AAGCTCGAAA ATCTGAGTCA 
GTTTATTATG CTGCAACAGA TGTGGTGATT CTTAGATTCA TGATTGAGGT ATGCTGGGCT 
CCTATGCTGG CAGCCTTCAG TGTGCCTCTT GACCAAAGTG ATGATGAAGT GGTGATAGCT 
TTGTGTCTTG AAGGTATCCG CTATGCAATC CATGTTACTG CTGTGATGTC CATGAAGACT 
CATAGAGATG CTTTTGTGAC TTCATTAGCA AAGTTTACAT CGCTCCACTC CCCTGCTGAT 
ATAAAGCAGA AAAATATTGA TGCTATCAAG GCAATAGTTA CAATTGCAGA TGAAGATGGG 
AATTATTTGC AAGAAGCTTG GGAACATATT TTGACATGTG TCTCACGCTT TGAACATTTG 
CATCTCCTGG GAGAGGGTGC TCCTCCAGAT GCCACTTTTT TTGCTTTTCC CCAAAATAAC 
TCTGAAAAGT CAAAACAAAG CAAGTCAACC ATCCTCCCTG TTCTGAAAAA GAAGGGACCC 
GGGAGGATGC AGCATGCAGC TGCTAGTGTG CTGAGAGGTT CATATGATAG TGCTGGTATT 
GGTGGAAACG CTGCTGGGGC TGTCACAAGT GAGCAGATGA ACAATTTAGT TTCCAACTTA 
AACAAGTTAG AGCAAGTTGG CAGCTCTGAG ATGAACCGCA TCTTCACACG GAGCCAAAAG 
TTAAACAGTG AGGCAATAAT TGACTTTGTG AAGGCTCTTT GCAAGGTTTC TGTGGAGGAA 
TTGAGATCAG CTTCTGATCC ACGGGTCTTT AGCCTTACAA AGATTGTTGA GATTGCGCAC 
TTTAACATGA ATCGCATCAG GCTTGTATGG TCAAGCATCT GGCATGTGCT TTCTGATTTC 
TTTGTAACCA TTGGCTGTTC TGAAAACCTT TCCATTGCAA TTTTTGCAAT GGACTCTTTA 
CGCCAATTAT CAATGAAATT CTTAGACCGA GAAGAGTTGG CAAACTATAA TTTTCAGAAT 
GAGTTTATGA AGCCATTTGT TATTGTTATG CGGAAGAGTA ATGCTGTTGA AATCAGAGAA 
TTGATTATCA GATGTGTCTC GCAAATGGTC CTGTCTCGTG TCAATAATGT TAAATCTGGA 
TGGAAGAGCA TGTTCATGGT TTTCACAGCA GCAGCTTATG ATGACCACAA AAACATTGTA 
CTCTTGGCCT TTGAAATAAT TGAAAAGATT ATTAGAGATT ACTTCCCATA CATTACGGAG 
ACTGAAACAA CCACCTTTAC TGATTGTGTG AATTGCCTGA TAGCATTCAC CAATAGTAGA 
TTCAACAAAG ACATTAGCCT TAATGCAATT GCTTTTCTTC AATTCTGTGC CACAAAACTT 
GCTGAAGGAG ATCTTGGATC TTCATCAAGG AACAAAGACA AGGAAGTCTC TGTAAAAATT 
TCATCCCCTT CACCTCGGAC CGGAAAAGAT GGAAAACAAG AGAATGGAGA AATCAAAGAC 
AAGGAAGATC ATCTTTATTT CTGGTTCCCT CTGTTGGCTG GTTTATCAGA ACTTAGCTTT 
GACCCTAGGC CTGAAGTCAG GAAGAGTGCC TTGCAAGTTC TGTTTGAAAC TTTACGCAAT 
CATGGTCATC TTTTTTCTCT ACCTTTATGG GAAAGAGTCT TTGAGTCGGT CTTATTTCCT 
ATATTTGACT ATGTGCGGCA TGCTATTGAT CCTCCTGGAG GCAACTCCCC TGAGCAGGGG 
ATTGATGGTG ATATGGGTGA ACTTGATCAA GATGCATGGC TCTATGGGAC ATGTACCTTG 
GCTCTCCAAC TGGTTGTAGA TCTTTTTGTG AAATTCTACA ATACTGTCAA TCCGCTTTTA 
AGAAAGGTGC TATCGCTTCT GGTTAGCTTC ATTAGGCGTC CTCACCAAAG TCTTGCTGGT 
ATTGGTATTG CGGCATTTGT GCGTTTGATG AGCAATGCTG GAGATATGTT TTCAGAGGAG 
AAGTGGCTGG AAGTGGTTTT GTCATTGAAA GATGCTGCCA ATGCAACACT CCCTGATTTC 
TCTTACATTG TAAGTGGAGA ATCATCGGTA ATAGCCGATG AACAAAATAA TGGAGAGACT 
GCTGGATCTG ACATGCCCGA AGATGAATCA GAGGGACTGG TGACACACCA TCTTTATGCT 
TCTATATCTG ATGCAAAGTG TCGAGCTGCT GTTCAACTTC TGTTGATTCA GGCGGTGATG 
GAGATCTATA GCATGTACCG GTCTCAACTT TCAGCAAAAT GTGCCTTGGT GCTCTTTGAT 
GCTTTGCATG AGGTGGCATC CCATGCTCAC AGTATCAACA CAAATACAAC ATTACGTTCA 
AAACTACAAG AGTTTGGCTC TATGACCCAA ATGCAAGACC CTCCACTATT ACGCCTGGAG 
AATGAGTCCT ATCAAATTTG CCTCACATTC TTACAAAATC TTATGATGGA CAGACCTCCA 
CCTTTTGATG AAGCTGAAGT GGAATCCTGC CTTGTCAACC TCTGTGAAGA GGTTTTACAG 
TTTTACGTTG TAACTGCCTG TTCTGGACAG GCATCAGAAA CTTCTACCAG TGGACAATGC 
CTGTGGCTGA TTCCTTTAGG TTCTGGAAAA CGAAGGGAAT TGGCTGCACG TGCACCTCTT 
ATTGTTGCAA CTCTCCAGGC CATCTGTAGT TTGGGAGACT CTTCGTTTGA GAAGAAATTG 
CCTCATTTCT TCCCTCTTCT TTCGAGTTTG ATAAGTTGTG AGCATGGGTC AAATGAGGTC 
CAGGTAGCTC TCAGTGACAT GCTCAGCTCA TCAGTGGGTC CTGTTTTGCT TCGGTCTTGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0002s15020g ( XM_002301262.2 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_002301298.2 Populus trichocarpa hypothetical protein (POPTR_0002s15020g) mRNA, complete cds.

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CloneID	OPa06520
Clone ID Related Accession (Same CDS sequence)	XM_002301262.2
Accession Version	XM_002301262.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1388332800000
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008468). On Dec 16, 2013 this sequence version replaced XM_002301262.1. COMPLETENESS: incomplete on the 5' end.

ATGGCTTCCT CGGAAGCCGA TTCCCGGTTA AGCCAAGTGG TATCCCCTGC TTTAGAAAAA 
ATCGTCAAAA ACGCATCGTG GCGTAAACAC TCGAAATTAG CACACGAATG CAAATCCGTC 
CTCGAGATTC TCACTTCTCG AAAACCCCAA CAACAACATC CTCCGACTTC CCCGTCGGAC 
GACTCCTCAT CCGAATCCTC TCTCCCTGGC CCACTCCACG ACGGCGGGTC CATCGAGTAC 
TCCCTCGCCG AATCTGAATC TATCCTCAGC CCTCTGATCA ACGCGTGCAA CACTCAATTC 
CTCAAGATCG TCGATCCAGC CGTTGATTGC ATCCAGAAAT TGATCGCGCA TGGGTACCTA 
CGTGGCGAGG CGGACTCCAC CGGAGGTACT GAAGCGAAAT TGCTAGCTAA GTTGATTGAA 
TCTGTGTGTA AATGTTATGA TTTAGGGGAT GATGGTGCTG AATTGCTGGT TTTGAAAACC 
CTATTATCAG CCGTTACGTC GATTTCGTTG AGAATTCATG GAGATTGTTT GTTACAAATA 
GTGAGAACTT GTTATGACAT TTATTTAGGG AGTAAAAATG TAATTAATCA AACGACGGCG 
AAAGCGTCTT TGATTCAAAT GTTAGTTATT GTGTTTAGGA GAATGGAGGC TGATTCTTCA 
ACGGTTCCGG TTCAGCCGAT TGTTGTGGCT GAATTAATGG AACCAGTGGA GAAAACGGAT 
GTCGATGGAT CAATGGCGGT GTTTGTTCAA GGTTTTATAA CGAAAATTAT GCAGGATATA 
GATGGGGTTT TTAATCCGGG GACACCCAGT AAGTCTTCGA TGACAGTGGC ACATGATGGG 
GCTTTCGAGA CCACTACTGG TACGGTGGAG AGTACAAATC CGGCAGATTT GCTTGATTCG 
ACGGATAAGG ATATGTTAGA TGCCAAATAT TGGGAAATTA GTATGTATAA GACGGCTTTG 
GAAGGCAGGA AAGGGGAGTT GGCTGATGGG GAAGGGGAGA GGGAGGATGA TTTGGAAGTG 
CAGATTGGGA ATAAGTTGAG GAGAGATGCA TTTTTGGTGT TTCGAGCACT TTGTAAGTTA 
TCTATGAAAA CTCCGCCCAA AGAAGCATTG GCGGATCCAC AGTTGATGAG AGGGAAGATT 
GTTGCATTGG AGTTATTGAA GATTTTGTTG GAGAATGCTG GTGCTGTGTT TAGGACTAGT 
GACAGGTTCT TAGGTGCCAT CAAGCAATAT CTATGTCTTT CACTGTTGAA GAACAGCTCG 
TCAAGTCTTA TGATCATTTT CCAGCTTTCT TGCTCCATTT TCATTAGTTT GGTTTCAAGG 
TTCAGAGCTG GACTGAAAGC AGAAATTGGA GTATTTTTTC CTATGATTGT TCTCAGGATT 
CTTGAGAATG TTGTTCAACC TAATTTTCAG CAGAAGATTA TAGTACTTCG GTTTCTAGAT 
AAGCTCTGTG TTGATTCCCA GATCTTGGTA GACATTTTTA TTAATTATGA CTGTGATATC 
AACTCATCAA ACATATTTGA GAGAATGGTC AATGGACTTC TTAAAACTGC TCAAGGTGCC 
CTGCCTGGTA CTGCCACTAC ACTGGTGCCA CCTCAGGAGG TGACCATGAA ACTTGAAGCT 
ATGAAGTCCT TAGTGGCTAT TCTGAAATCA ATGGGAGACT GGATGAATAA ACAATTGCGC 
ATTCCCGATC CTCATTCTGC CAAGAAATCT GATGCAGCCG AAAACAGTCC TGGACCTGGA 
AGTCTTCCCA TGACAAATGG TAATGGGGAT GAGCCTGTTG AAGGATCAGA TTCTCATTCT 
GAAACCTCAA CTGAAGCATC GGATGTTTCA GCTATTGAGC AACGACGGGC TTACAAGCTG 
GAATTTCAGG AAGGTATATC TCTTTTCAAT CGGAAGCCTA AGAAAGGAAT TGAATTTCTA 
ATCAATGCAA ATAAAGTGGG GAATTCAGCA GAAGAGATAG CAGCCTTTCT TAAAAACGCA 
TCTGGCTTGA ACAAGACTCT GATTGGCGAT TACCTTGGGG AAAGAGAAGA TTTTTCACTG 
AAAGTAATGC ATGCTTATGT AGATTCTTTT GACTTTCGCG GCCTGGAGTT CGATGAGGCT 
ATCAGAGTCT TTCTACAGGG CTTTAGGTTA CCAGGTGAGG CACAAAAGAT TGATCGAATT 
ATGGAGAAGT TTGCTGAACG TTACTGCAAA TGTAACCCAA AGGTTTTTAG CAGTGCTGAC 
ACAGCCTATG TCCTTGCTTA CTCCGTGATA CTGCTCAACA CTGATGCTCA TAATCCCATG 
GTGAAGAATA AGATGTCAGC TGATGACTTC ATAAGAAACA ACCGTGGCAT TGATGATGGG 
AAAGATCTAC CTGAGGAGTA CTTGAGATCT TTGTTTGAAA GAATATCAAA AAATGAGATC 
AAAATGAAAG AATATGATTT AGCTCTCCAA CAAAAGCAGT CTTTGAATTC AAACAGAGTT 
TTAGGCTTGG ATAGTATCCT GAACATTGTG ATCCGCAAGC GGGGTGAAGA AAAGAATATG 
GAAACAAGTG ATGATCTTAT CAGGCACATG CAGGAACAAT TTAAAGAAAA AGCTCGAAAA 
TCTGAGTCAG TTTATTATGC TGCAACAGAT GTGGTGATTC TTAGATTCAT GATTGAGGTA 
TGCTGGGCTC CTATGCTGGC AGCCTTCAGT GTGCCTCTTG ACCAAAGTGA TGATGAAGTG 
GTGATAGCTT TGTGTCTTGA AGGTATCCGC TATGCAATCC ATGTTACTGC TGTGATGTCC 
ATGAAGACTC ATAGAGATGC TTTTGTGACT TCATTAGCAA AGTTTACATC GCTCCACTCC 
CCTGCTGATA TCAAGCAGAA AAATATTGAT GCTATCAAGG CAATAGTTAC AATTGCAGAT 
GAAGATGGGA ATTATTTGCA AGAAGCTTGG GAACATATTT TGACATGTGT CTCACGCTTT 
GAACATTTGC ATCTCCTGGG AGAGGGTGCT CCTCCAGATG CCACTTTTTT TGCTTTTCCC 
CAAAATAACT CTGAAAAGTC AAAACAAAGC AAGTCAACCA TCCTCCCTGT TCTGAAAAAG 
AAGGGACCCG GGAGGATGCA GCATGCAGCT GCTAGTGTGC TGAGAGGTTC ATATGATAGT 
GCTGGTATTG GTGGAAACGC TGCTGGGGCT GTCACAAGTG AGCAGATGAA CAATTTAGTT 
TCCAACTTAA ACAAGTTAGA GCAAGTTGGC AGCTCTGAGA TGAACCGCAT CTTCACACGG 
AGCCAAAAGT TAAACAGTGA GGCAATAATT GACTTTGTGA AGGCTCTTTG CAAGGTTTCT 
GTGGAGGAAT TGAGATCAGC TTCTGATCCA CGGGTCTTTA GCCTTACAAA GATTGTTGAG 
ATTGCGCACT TTAACATGAA TCGCATCAGG CTTGTATGGT CAAGCATCTG GCATGTGCTT 
TCTGATTTCT TTGTAACCAT TGGCTGTTCT GAAAACCTTT CCATTGCAAT TTTTGCAATG 
GACTCTTTAC GCCAATTATC AATGAAATTC TTAGACCGAG AAGAGTTGGC AAACTATAAT 
TTTCAGAATG AGTTTATGAA GCCATTTGTT ATTGTTATGC GGAAGAGTAA TGCTGTTGAA 
ATCAGAGAAT TGATTATCAG ATGTGTCTCG CAAATGGTCC TGTCTCGTGT CAATAATGTT 
AAATCTGGAT GGAAGAGCAT GTTCATGGTT TTCACAGCAG CAGCTTATGA TGACCACAAA 
AACATTGTAC TCTTGGCCTT TGAAATAATT GAAAAGATTA TTAGAGATTA CTTCCCATAC 
ATTACGGAGA CTGAAACAAC CACCTTTACT GATTGTGTGA ATTGCCTGAT AGCATTCACC 
AATAGTAGAT TCAACAAAGA CATTAGCCTT AATGCAATTG CTTTTCTTCA ATTCTGTGCC 
ACAAAACTTG CTGAAGGAGA TCTTGGATCT TCATCAAGGA ACAAAGACAA GGAAGTCTCT 
GTAAAAATTT CATCCCCTTC ACCTCGGACT GGAAAAGATG GAAAACAAGA GAATGGAGAA 
ATCAAAGACA AGGAAGATCA TCTTTATTTC TGGTTCCCTC TGTTGGCTGG TTTATCAGAA 
CTTAGCTTTG ACCCTAGGCC TGAAGTCAGG AAGAGTGCCT TGCAAGTTCT GTTTGAAACT 
TTACGCAATC ATGGTCATCT TTTTTCTCTA CCTTTATGGG AAAGAGTCTT TGAGTCGGTC 
TTATTTCCTA TATTTGACTA TGTGCGGCAT GCTATTGATC CTCCTGGAGG CAACTCCCCT 
GAGCAGGGGA TTGATGGTGA TATGGGTGAA CTTGATCAAG ATGCATGGCT CTATGGGACA 
TGTACCTTGG CTCTCCAACT GGTTGTAGAT CTTTTTGTGA AATTCTACAA TACTGTCAAT 
CCGCTTTTAA GAAAGGTGCT ATCGCTTCTG GTTAGCTTCA TTAGGCGTCC TCACCAAAGT 
CTTGCTGGTA TTGGTATTGC GGCATTTGTG CGTTTGATGA GCAATGCTGG AGATATGTTT 
TCAGAGGAGA AGTGGCTGGA AGTGGTTTTG TCATTGAAAG ATGCTGCCAA TGCAACACTC 
CCTGATTTCT CTTACATTGT AAGTGGAGAA TCATCGGTAA TAGCCGATGA ACAAAATAAT 
GGAGAGACTG CTGGATCTGA CATGCCCGAA GATGAATCAG AGGGACTGGT GACACACCGT 
CTTTATGCTT CTATATCTGA TGCAAAGTGT CGAGCTGCTG TTCAACTTCT GTTGATTCAG 
GCGGTGATGG AGATCTATAG CATGTACCGG TCTCAACTTT CAGCAAAATG TGCCTTGGTG 
CTCTTTGATG CTTTGCATGA GGTGGCATCC CATGCTCACA GTATCAACAC AAATACAACA 
TTACGTTCAA AACTACAAGA GTTTGGCTCT ATGACCCAAA TGCAAGACCC TCCACTATTA 
CGCCTGGAGA ATGAGTCCTA TCAAATTTGC CTCACATTCT TACAAAATCT TATGCTGGAC 
AGACCTCCAC CTTTTGATGA AGCTGAAGTG GAATCCTGCC TTGTCAACCT CTGTGAAGAG 
GTTTTACAGT TTTACGTTGT AACTGCCTGT TCTGGACAGG CATCAGAAAC TTCTACCAGT 
GGACAATGCC TGTGGCTGAT TCCTTTAGGT TCTGGAAAAC GAAGGGAATT GGCTGCACGT 
GCACCTCTTA TTGTTGCAAC TCTCCAGGCC ATCTGTAGTT TGGGAGACTC TTCGTTTGAG 
AAGAAATTGC CTCATTTCTT CCCTCTTCTT TCGAGTTTGA TAAGTTGTGA GCATGGGTCA 
AATGAGGTCC AGGTAGCTCT CAGTGACATG CTCAGCTCAT CAGTGGGTCC TGTTTTGCTT 
CGGTCTTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002301298.2
CDS	1..5352
Translation	MASSEADSRLSQVVSPALEKIVKNASWRKHSKLAHECKSVLEILTSRKPQQQHPPTSPSDDSSSESSLPGPLHDGGSIEYSLAESESILSPLINACNTQFLKIVDPAVDCIQKLIAHGYLRGEADSTGGTEAKLLAKLIESVCKCYDLGDDGAELLVLKTLLSAVTSISLRIHGDCLLQIVRTCYDIYLGSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPVEKTDVDGSMAVFVQGFITKIMQDIDGVFNPGTPSKSSMTVAHDGAFETTTGTVESTNPADLLDSTDKDMLDAKYWEISMYKTALEGRKGELADGEGEREDDLEVQIGNKLRRDAFLVFRALCKLSMKTPPKEALADPQLMRGKIVALELLKILLENAGAVFRTSDRFLGAIKQYLCLSLLKNSSSSLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRILENVVQPNFQQKIIVLRFLDKLCVDSQILVDIFINYDCDINSSNIFERMVNGLLKTAQGALPGTATTLVPPQEVTMKLEAMKSLVAILKSMGDWMNKQLRIPDPHSAKKSDAAENSPGPGSLPMTNGNGDEPVEGSDSHSETSTEASDVSAIEQRRAYKLEFQEGISLFNRKPKKGIEFLINANKVGNSAEEIAAFLKNASGLNKTLIGDYLGEREDFSLKVMHAYVDSFDFRGLEFDEAIRVFLQGFRLPGEAQKIDRIMEKFAERYCKCNPKVFSSADTAYVLAYSVILLNTDAHNPMVKNKMSADDFIRNNRGIDDGKDLPEEYLRSLFERISKNEIKMKEYDLALQQKQSLNSNRVLGLDSILNIVIRKRGEEKNMETSDDLIRHMQEQFKEKARKSESVYYAATDVVILRFMIEVCWAPMLAAFSVPLDQSDDEVVIALCLEGIRYAIHVTAVMSMKTHRDAFVTSLAKFTSLHSPADIKQKNIDAIKAIVTIADEDGNYLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNNSEKSKQSKSTILPVLKKKGPGRMQHAAASVLRGSYDSAGIGGNAAGAVTSEQMNNLVSNLNKLEQVGSSEMNRIFTRSQKLNSEAIIDFVKALCKVSVEELRSASDPRVFSLTKIVEIAHFNMNRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFMKPFVIVMRKSNAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTAAAYDDHKNIVLLAFEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNSRFNKDISLNAIAFLQFCATKLAEGDLGSSSRNKDKEVSVKISSPSPRTGKDGKQENGEIKDKEDHLYFWFPLLAGLSELSFDPRPEVRKSALQVLFETLRNHGHLFSLPLWERVFESVLFPIFDYVRHAIDPPGGNSPEQGIDGDMGELDQDAWLYGTCTLALQLVVDLFVKFYNTVNPLLRKVLSLLVSFIRRPHQSLAGIGIAAFVRLMSNAGDMFSEEKWLEVVLSLKDAANATLPDFSYIVSGESSVIADEQNNGETAGSDMPEDESEGLVTHRLYASISDAKCRAAVQLLLIQAVMEIYSMYRSQLSAKCALVLFDALHEVASHAHSINTNTTLRSKLQEFGSMTQMQDPPLLRLENESYQICLTFLQNLMLDRPPPFDEAEVESCLVNLCEEVLQFYVVTACSGQASETSTSGQCLWLIPLGSGKRRELAARAPLIVATLQAICSLGDSSFEKKLPHFFPLLSSLISCEHGSNEVQVALSDMLSSSVGPVLLRSC

Target ORF information:

RefSeq Version	XM_002301262.2
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa hypothetical protein (POPTR_0002s15020g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002301262.2

ORF Insert Sequence:

ATGGCTTCCT CGGAAGCCGA TTCCCGGTTA AGCCAAGTGG TATCCCCTGC TTTAGAAAAA 
ATCGTCAAAA ACGCATCGTG GCGTAAACAC TCGAAATTAG CACACGAATG CAAATCCGTC 
CTCGAGATTC TCACTTCTCG AAAACCCCAA CAACAACATC CTCCGACTTC CCCGTCGGAC 
GACTCCTCAT CCGAATCCTC TCTCCCTGGC CCACTCCACG ACGGCGGGTC CATCGAGTAC 
TCCCTCGCCG AATCTGAATC TATCCTCAGC CCTCTGATCA ACGCGTGCAA CACTCAATTC 
CTCAAGATCG TCGATCCAGC CGTTGATTGC ATCCAGAAAT TGATCGCGCA TGGGTACCTA 
CGTGGCGAGG CGGACTCCAC CGGAGGTACT GAAGCGAAAT TGCTAGCTAA GTTGATTGAA 
TCTGTGTGTA AATGTTATGA TTTAGGGGAT GATGGTGCTG AATTGCTGGT TTTGAAAACC 
CTATTATCAG CCGTTACGTC GATTTCGTTG AGAATTCATG GAGATTGTTT GTTACAAATA 
GTGAGAACTT GTTATGACAT TTATTTAGGG AGTAAAAATG TAATTAATCA AACGACGGCG 
AAAGCGTCTT TGATTCAAAT GTTAGTTATT GTGTTTAGGA GAATGGAGGC TGATTCTTCA 
ACGGTTCCGG TTCAGCCGAT TGTTGTGGCT GAATTAATGG AACCAGTGGA GAAAACGGAT 
GTCGATGGAT CAATGGCGGT GTTTGTTCAA GGTTTTATAA CGAAAATTAT GCAGGATATA 
GATGGGGTTT TTAATCCGGG GACACCCAGT AAGTCTTCGA TGACAGTGGC ACATGATGGG 
GCTTTCGAGA CCACTACTGG TACGGTGGAG AGTACAAATC CGGCAGATTT GCTTGATTCG 
ACGGATAAGG ATATGTTAGA TGCCAAATAT TGGGAAATTA GTATGTATAA GACGGCTTTG 
GAAGGCAGGA AAGGGGAGTT GGCTGATGGG GAAGGGGAGA GGGAGGATGA TTTGGAAGTG 
CAGATTGGGA ATAAGTTGAG GAGAGATGCA TTTTTGGTGT TTCGAGCACT TTGTAAGTTA 
TCTATGAAAA CTCCGCCCAA AGAAGCATTG GCGGATCCAC AGTTGATGAG AGGGAAGATT 
GTTGCATTGG AGTTATTGAA GATTTTGTTG GAGAATGCTG GTGCTGTGTT TAGGACTAGT 
GACAGGTTCT TAGGTGCCAT CAAGCAATAT CTATGTCTTT CACTGTTGAA GAACAGCTCG 
TCAAGTCTTA TGATCATTTT CCAGCTTTCT TGCTCCATTT TCATTAGTTT GGTTTCAAGG 
TTCAGAGCTG GACTGAAAGC AGAAATTGGA GTATTTTTTC CTATGATTGT TCTCAGGATT 
CTTGAGAATG TTGTTCAACC TAATTTTCAG CAGAAGATTA TAGTACTTCG GTTTCTAGAT 
AAGCTCTGTG TTGATTCCCA GATCTTGGTA GACATTTTTA TTAATTATGA CTGTGATATC 
AACTCATCAA ACATATTTGA GAGAATGGTC AATGGACTTC TTAAAACTGC TCAAGGTGCC 
CTGCCTGGTA CTGCCACTAC ACTGGTGCCA CCTCAGGAGG TGACCATGAA ACTTGAAGCT 
ATGAAGTCCT TAGTGGCTAT TCTGAAATCA ATGGGAGACT GGATGAATAA ACAATTGCGC 
ATTCCCGATC CTCATTCTGC CAAGAAATCT GATGCAGCCG AAAACAGTCC TGGACCTGGA 
AGTCTTCCCA TGACAAATGG TAATGGGGAT GAGCCTGTTG AAGGATCAGA TTCTCATTCT 
GAAACCTCAA CTGAAGCATC GGATGTTTCA GCTATTGAGC AACGACGGGC TTACAAGCTG 
GAATTTCAGG AAGGTATATC TCTTTTCAAT CGGAAGCCTA AGAAAGGAAT TGAATTTCTA 
ATCAATGCAA ATAAAGTGGG GAATTCAGCA GAAGAGATAG CAGCCTTTCT TAAAAACGCA 
TCTGGCTTGA ACAAGACTCT GATTGGCGAT TACCTTGGGG AAAGAGAAGA TTTTTCACTG 
AAAGTAATGC ATGCTTATGT AGATTCTTTT GACTTTCGCG GCCTGGAGTT CGATGAGGCT 
ATCAGAGTCT TTCTACAGGG CTTTAGGTTA CCAGGTGAGG CACAAAAGAT TGATCGAATT 
ATGGAGAAGT TTGCTGAACG TTACTGCAAA TGTAACCCAA AGGTTTTTAG CAGTGCTGAC 
ACAGCCTATG TCCTTGCTTA CTCCGTGATA CTGCTCAACA CTGATGCTCA TAATCCCATG 
GTGAAGAATA AGATGTCAGC TGATGACTTC ATAAGAAACA ACCGTGGCAT TGATGATGGG 
AAAGATCTAC CTGAGGAGTA CTTGAGATCT TTGTTTGAAA GAATATCAAA AAATGAGATC 
AAAATGAAAG AATATGATTT AGCTCTCCAA CAAAAGCAGT CTTTGAATTC AAACAGAGTT 
TTAGGCTTGG ATAGTATCCT GAACATTGTG ATCCGCAAGC GGGGTGAAGA AAAGAATATG 
GAAACAAGTG ATGATCTTAT CAGGCACATG CAGGAACAAT TTAAAGAAAA AGCTCGAAAA 
TCTGAGTCAG TTTATTATGC TGCAACAGAT GTGGTGATTC TTAGATTCAT GATTGAGGTA 
TGCTGGGCTC CTATGCTGGC AGCCTTCAGT GTGCCTCTTG ACCAAAGTGA TGATGAAGTG 
GTGATAGCTT TGTGTCTTGA AGGTATCCGC TATGCAATCC ATGTTACTGC TGTGATGTCC 
ATGAAGACTC ATAGAGATGC TTTTGTGACT TCATTAGCAA AGTTTACATC GCTCCACTCC 
CCTGCTGATA TCAAGCAGAA AAATATTGAT GCTATCAAGG CAATAGTTAC AATTGCAGAT 
GAAGATGGGA ATTATTTGCA AGAAGCTTGG GAACATATTT TGACATGTGT CTCACGCTTT 
GAACATTTGC ATCTCCTGGG AGAGGGTGCT CCTCCAGATG CCACTTTTTT TGCTTTTCCC 
CAAAATAACT CTGAAAAGTC AAAACAAAGC AAGTCAACCA TCCTCCCTGT TCTGAAAAAG 
AAGGGACCCG GGAGGATGCA GCATGCAGCT GCTAGTGTGC TGAGAGGTTC ATATGATAGT 
GCTGGTATTG GTGGAAACGC TGCTGGGGCT GTCACAAGTG AGCAGATGAA CAATTTAGTT 
TCCAACTTAA ACAAGTTAGA GCAAGTTGGC AGCTCTGAGA TGAACCGCAT CTTCACACGG 
AGCCAAAAGT TAAACAGTGA GGCAATAATT GACTTTGTGA AGGCTCTTTG CAAGGTTTCT 
GTGGAGGAAT TGAGATCAGC TTCTGATCCA CGGGTCTTTA GCCTTACAAA GATTGTTGAG 
ATTGCGCACT TTAACATGAA TCGCATCAGG CTTGTATGGT CAAGCATCTG GCATGTGCTT 
TCTGATTTCT TTGTAACCAT TGGCTGTTCT GAAAACCTTT CCATTGCAAT TTTTGCAATG 
GACTCTTTAC GCCAATTATC AATGAAATTC TTAGACCGAG AAGAGTTGGC AAACTATAAT 
TTTCAGAATG AGTTTATGAA GCCATTTGTT ATTGTTATGC GGAAGAGTAA TGCTGTTGAA 
ATCAGAGAAT TGATTATCAG ATGTGTCTCG CAAATGGTCC TGTCTCGTGT CAATAATGTT 
AAATCTGGAT GGAAGAGCAT GTTCATGGTT TTCACAGCAG CAGCTTATGA TGACCACAAA 
AACATTGTAC TCTTGGCCTT TGAAATAATT GAAAAGATTA TTAGAGATTA CTTCCCATAC 
ATTACGGAGA CTGAAACAAC CACCTTTACT GATTGTGTGA ATTGCCTGAT AGCATTCACC 
AATAGTAGAT TCAACAAAGA CATTAGCCTT AATGCAATTG CTTTTCTTCA ATTCTGTGCC 
ACAAAACTTG CTGAAGGAGA TCTTGGATCT TCATCAAGGA ACAAAGACAA GGAAGTCTCT 
GTAAAAATTT CATCCCCTTC ACCTCGGACT GGAAAAGATG GAAAACAAGA GAATGGAGAA 
ATCAAAGACA AGGAAGATCA TCTTTATTTC TGGTTCCCTC TGTTGGCTGG TTTATCAGAA 
CTTAGCTTTG ACCCTAGGCC TGAAGTCAGG AAGAGTGCCT TGCAAGTTCT GTTTGAAACT 
TTACGCAATC ATGGTCATCT TTTTTCTCTA CCTTTATGGG AAAGAGTCTT TGAGTCGGTC 
TTATTTCCTA TATTTGACTA TGTGCGGCAT GCTATTGATC CTCCTGGAGG CAACTCCCCT 
GAGCAGGGGA TTGATGGTGA TATGGGTGAA CTTGATCAAG ATGCATGGCT CTATGGGACA 
TGTACCTTGG CTCTCCAACT GGTTGTAGAT CTTTTTGTGA AATTCTACAA TACTGTCAAT 
CCGCTTTTAA GAAAGGTGCT ATCGCTTCTG GTTAGCTTCA TTAGGCGTCC TCACCAAAGT 
CTTGCTGGTA TTGGTATTGC GGCATTTGTG CGTTTGATGA GCAATGCTGG AGATATGTTT 
TCAGAGGAGA AGTGGCTGGA AGTGGTTTTG TCATTGAAAG ATGCTGCCAA TGCAACACTC 
CCTGATTTCT CTTACATTGT AAGTGGAGAA TCATCGGTAA TAGCCGATGA ACAAAATAAT 
GGAGAGACTG CTGGATCTGA CATGCCCGAA GATGAATCAG AGGGACTGGT GACACACCGT 
CTTTATGCTT CTATATCTGA TGCAAAGTGT CGAGCTGCTG TTCAACTTCT GTTGATTCAG 
GCGGTGATGG AGATCTATAG CATGTACCGG TCTCAACTTT CAGCAAAATG TGCCTTGGTG 
CTCTTTGATG CTTTGCATGA GGTGGCATCC CATGCTCACA GTATCAACAC AAATACAACA 
TTACGTTCAA AACTACAAGA GTTTGGCTCT ATGACCCAAA TGCAAGACCC TCCACTATTA 
CGCCTGGAGA ATGAGTCCTA TCAAATTTGC CTCACATTCT TACAAAATCT TATGCTGGAC 
AGACCTCCAC CTTTTGATGA AGCTGAAGTG GAATCCTGCC TTGTCAACCT CTGTGAAGAG 
GTTTTACAGT TTTACGTTGT AACTGCCTGT TCTGGACAGG CATCAGAAAC TTCTACCAGT 
GGACAATGCC TGTGGCTGAT TCCTTTAGGT TCTGGAAAAC GAAGGGAATT GGCTGCACGT 
GCACCTCTTA TTGTTGCAAC TCTCCAGGCC ATCTGTAGTT TGGGAGACTC TTCGTTTGAG 
AAGAAATTGC CTCATTTCTT CCCTCTTCTT TCGAGTTTGA TAAGTTGTGA GCATGGGTCA 
AATGAGGTCC AGGTAGCTCT CAGTGACATG CTCAGCTCAT CAGTGGGTCC TGTTTTGCTT 
CGGTCTTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0002s15020g ( XM_002301262.3 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_002301298.3 Populus trichocarpa brefeldin A-inhibited guanine nucleotide-exchange protein 2 (LOC7494008), transcript variant X1, mRNA.

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CloneID	OPa424977
Clone ID Related Accession (Same CDS sequence)	XM_002301262.3
Accession Version	XM_002301262.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037286.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 9, 2018 this sequence version replaced XM_002301262.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCCT CGGAAGCCGA TTCCCGGTTA AGCCAAGTGG TATCCCCTGC TTTAGAAAAA 
ATCGTCAAAA ACGCATCGTG GCGTAAACAC TCGAAATTAG CACACGAATG CAAATCCGTC 
CTCGAGATTC TCACTTCTCG AAAACCCCAA CAACAACATC CTCCGACTTC CCCGTCGGAC 
GACTCCTCAT CCGAATCCTC TCTCCCTGGC CCACTCCACG ACGGCGGGTC CATCGAGTAC 
TCCCTCGCCG AATCTGAATC TATCCTCAGC CCTCTGATCA ACGCGTGCAA CACTCAATTC 
CTCAAGATCG TCGATCCAGC CGTTGATTGC ATCCAGAAAT TGATCGCGCA TGGGTACCTA 
CGTGGCGAGG CGGACTCCAC CGGAGGTACT GAAGCGAAAT TGCTAGCTAA GTTGATTGAA 
TCTGTGTGTA AATGTTATGA TTTAGGGGAT GATGGTGCTG AATTGCTGGT TTTGAAAACC 
CTATTATCAG CCGTTACGTC GATTTCGTTG AGAATTCATG GAGATTGTTT GTTACAAATA 
GTGAGAACTT GTTATGACAT TTATTTAGGG AGTAAAAATG TAATTAATCA AACGACGGCG 
AAAGCGTCTT TGATTCAAAT GTTAGTTATT GTGTTTAGGA GAATGGAGGC TGATTCTTCA 
ACGGTTCCGG TTCAGCCGAT TGTTGTGGCT GAATTAATGG AACCAGTGGA GAAAACGGAT 
GTCGATGGAT CAATGGCGGT GTTTGTTCAA GGTTTTATAA CGAAAATTAT GCAGGATATA 
GATGGGGTTT TTAATCCGGG GACACCCAGT AAGTCTTCGA TGACAGTGGC ACATGATGGG 
GCTTTCGAGA CCACTACTGG TACGGTGGAG AGTACAAATC CGGCAGATTT GCTTGATTCG 
ACGGATAAGG ATATGTTAGA TGCCAAATAT TGGGAAATTA GTATGTATAA GACGGCTTTG 
GAAGGCAGGA AAGGGGAGTT GGCTGATGGG GAAGGGGAGA GGGAGGATGA TTTGGAAGTG 
CAGATTGGGA ATAAGTTGAG GAGAGATGCA TTTTTGGTGT TTCGAGCACT TTGTAAGTTA 
TCTATGAAAA CTCCGCCCAA AGAAGCATTG GCGGATCCAC AGTTGATGAG AGGGAAGATT 
GTTGCATTGG AGTTATTGAA GATTTTGTTG GAGAATGCTG GTGCTGTGTT TAGGACTAGT 
GACAGGTTCT TAGGTGCCAT CAAGCAATAT CTATGTCTTT CACTGTTGAA GAACAGCTCG 
TCAAGTCTTA TGATCATTTT CCAGCTTTCT TGCTCCATTT TCATTAGTTT GGTTTCAAGG 
TTCAGAGCTG GACTGAAAGC AGAAATTGGA GTATTTTTTC CTATGATTGT TCTCAGGATT 
CTTGAGAATG TTGTTCAACC TAATTTTCAG CAGAAGATTA TAGTACTTCG GTTTCTAGAT 
AAGCTCTGTG TTGATTCCCA GATCTTGGTA GACATTTTTA TTAATTATGA CTGTGATATC 
AACTCATCAA ACATATTTGA GAGAATGGTC AATGGACTTC TTAAAACTGC TCAAGGTGCC 
CTGCCTGGTA CTGCCACTAC ACTGGTGCCA CCTCAGGAGG TGACCATGAA ACTTGAAGCT 
ATGAAGTCCT TAGTGGCTAT TCTGAAATCA ATGGGAGACT GGATGAATAA ACAATTGCGC 
ATTCCCGATC CTCATTCTGC CAAGAAATCT GATGCAGCCG AAAACAGTCC TGGACCTGGA 
AGTCTTCCCA TGACAAATGG TAATGGGGAT GAGCCTGTTG AAGGATCAGA TTCTCATTCT 
GAAACCTCAA CTGAAGCATC GGATGTTTCA GCTATTGAGC AACGACGGGC TTACAAGCTG 
GAATTTCAGG AAGGTATATC TCTTTTCAAT CGGAAGCCTA AGAAAGGAAT TGAATTTCTA 
ATCAATGCAA ATAAAGTGGG GAATTCAGCA GAAGAGATAG CAGCCTTTCT TAAAAACGCA 
TCTGGCTTGA ACAAGACTCT GATTGGCGAT TACCTTGGGG AAAGAGAAGA TTTTTCACTG 
AAAGTAATGC ATGCTTATGT AGATTCTTTT GACTTTCGCG GCCTGGAGTT CGATGAGGCT 
ATCAGAGTCT TTCTACAGGG CTTTAGGTTA CCAGGTGAGG CACAAAAGAT TGATCGAATT 
ATGGAGAAGT TTGCTGAACG TTACTGCAAA TGTAACCCAA AGGTTTTTAG CAGTGCTGAC 
ACAGCCTATG TCCTTGCTTA CTCCGTGATA CTGCTCAACA CTGATGCTCA TAATCCCATG 
GTGAAGAATA AGATGTCAGC TGATGACTTC ATAAGAAACA ACCGTGGCAT TGATGATGGG 
AAAGATCTAC CTGAGGAGTA CTTGAGATCT TTGTTTGAAA GAATATCAAA AAATGAGATC 
AAAATGAAAG AATATGATTT AGCTCTCCAA CAAAAGCAGT CTTTGAATTC AAACAGAGTT 
TTAGGCTTGG ATAGTATCCT GAACATTGTG ATCCGCAAGC GGGGTGAAGA AAAGAATATG 
GAAACAAGTG ATGATCTTAT CAGGCACATG CAGGAACAAT TTAAAGAAAA AGCTCGAAAA 
TCTGAGTCAG TTTATTATGC TGCAACAGAT GTGGTGATTC TTAGATTCAT GATTGAGGTA 
TGCTGGGCTC CTATGCTGGC AGCCTTCAGT GTGCCTCTTG ACCAAAGTGA TGATGAAGTG 
GTGATAGCTT TGTGTCTTGA AGGTATCCGC TATGCAATCC ATGTTACTGC TGTGATGTCC 
ATGAAGACTC ATAGAGATGC TTTTGTGACT TCATTAGCAA AGTTTACATC GCTCCACTCC 
CCTGCTGATA TAAAGCAGAA AAATATTGAT GCTATCAAGG CAATAGTTAC AATTGCAGAT 
GAAGATGGGA ATTATTTGCA AGAAGCTTGG GAACATATTT TGACATGTGT CTCACGCTTT 
GAACATTTGC ATCTCCTGGG AGAGGGTGCT CCTCCAGATG CCACTTTTTT TGCTTTTCCC 
CAAAATAACT CTGAAAAGTC AAAACAAAGC AAGTCAACCA TCCTCCCTGT TCTGAAAAAG 
AAGGGACCCG GGAGGATGCA GCATGCAGCT GCTAGTGTGC TGAGAGGTTC ATATGATAGT 
GCTGGTATTG GTGGAAACGC TGCTGGGGCT GTCACAAGTG AGCAGATGAA CAATTTAGTT 
TCCAACTTAA ACAAGTTAGA GCAAGTTGGC AGCTCTGAGA TGAACCGCAT CTTCACACGG 
AGCCAAAAGT TAAACAGTGA GGCAATAATT GACTTTGTGA AGGCTCTTTG CAAGGTTTCT 
GTGGAGGAAT TGAGATCAGC TTCTGATCCA CGGGTCTTTA GCCTTACAAA GATTGTTGAG 
ATTGCGCACT TTAACATGAA TCGCATCAGG CTTGTATGGT CAAGCATCTG GCATGTGCTT 
TCTGATTTCT TTGTAACCAT TGGCTGTTCT GAAAACCTTT CCATTGCAAT TTTTGCAATG 
GACTCTTTAC GCCAATTATC AATGAAATTC TTAGACCGAG AAGAGTTGGC AAACTATAAT 
TTTCAGAATG AGTTTATGAA GCCATTTGTT ATTGTTATGC GGAAGAGTAA TGCTGTTGAA 
ATCAGAGAAT TGATTATCAG ATGTGTCTCG CAAATGGTCC TGTCTCGTGT CAATAATGTT 
AAATCTGGAT GGAAGAGCAT GTTCATGGTT TTCACAGCAG CAGCTTATGA TGACCACAAA 
AACATTGTAC TCTTGGCCTT TGAAATAATT GAAAAGATTA TTAGAGATTA CTTCCCATAC 
ATTACGGAGA CTGAAACAAC CACCTTTACT GATTGTGTGA ATTGCCTGAT AGCATTCACC 
AATAGTAGAT TCAACAAAGA CATTAGCCTT AATGCAATTG CTTTTCTTCA ATTCTGTGCC 
ACAAAACTTG CTGAAGGAGA TCTTGGATCT TCATCAAGGA ACAAAGACAA GGAAGTCTCT 
GTAAAAATTT CATCCCCTTC ACCTCGGACC GGAAAAGATG GAAAACAAGA GAATGGAGAA 
ATCAAAGACA AGGAAGATCA TCTTTATTTC TGGTTCCCTC TGTTGGCTGG TTTATCAGAA 
CTTAGCTTTG ACCCTAGGCC TGAAGTCAGG AAGAGTGCCT TGCAAGTTCT GTTTGAAACT 
TTACGCAATC ATGGTCATCT TTTTTCTCTA CCTTTATGGG AAAGAGTCTT TGAGTCGGTC 
TTATTTCCTA TATTTGACTA TGTGCGGCAT GCTATTGATC CTCCTGGAGG CAACTCCCCT 
GAGCAGGGGA TTGATGGTGA TATGGGTGAA CTTGATCAAG ATGCATGGCT CTATGGGACA 
TGTACCTTGG CTCTCCAACT GGTTGTAGAT CTTTTTGTGA AATTCTACAA TACTGTCAAT 
CCGCTTTTAA GAAAGGTGCT ATCGCTTCTG GTTAGCTTCA TTAGGCGTCC TCACCAAAGT 
CTTGCTGGTA TTGGTATTGC GGCATTTGTG CGTTTGATGA GCAATGCTGG AGATATGTTT 
TCAGAGGAGA AGTGGCTGGA AGTGGTTTTG TCATTGAAAG ATGCTGCCAA TGCAACACTC 
CCTGATTTCT CTTACATTGT AAGTGGAGAA TCATCGGTAA TAGCCGATGA ACAAAATAAT 
GGAGAGACTG CTGGATCTGA CATGCCCGAA GATGAATCAG AGGGACTGGT GACACACCAT 
CTTTATGCTT CTATATCTGA TGCAAAGTGT CGAGCTGCTG TTCAACTTCT GTTGATTCAG 
GCGGTGATGG AGATCTATAG CATGTACCGG TCTCAACTTT CAGCAAAATG TGCCTTGGTG 
CTCTTTGATG CTTTGCATGA GGTGGCATCC CATGCTCACA GTATCAACAC AAATACAACA 
TTACGTTCAA AACTACAAGA GTTTGGCTCT ATGACCCAAA TGCAAGACCC TCCACTATTA 
CGCCTGGAGA ATGAGTCCTA TCAAATTTGC CTCACATTCT TACAAAATCT TATGATGGAC 
AGACCTCCAC CTTTTGATGA AGCTGAAGTG GAATCCTGCC TTGTCAACCT CTGTGAAGAG 
GTTTTACAGT TTTACGTTGT AACTGCCTGT TCTGGACAGG CATCAGAAAC TTCTACCAGT 
GGACAATGCC TGTGGCTGAT TCCTTTAGGT TCTGGAAAAC GAAGGGAATT GGCTGCACGT 
GCACCTCTTA TTGTTGCAAC TCTCCAGGCC ATCTGTAGTT TGGGAGACTC TTCGTTTGAG 
AAGAAATTGC CTCATTTCTT CCCTCTTCTT TCGAGTTTGA TAAGTTGTGA GCATGGGTCA 
AATGAGGTCC AGGTAGCTCT CAGTGACATG CTCAGCTCAT CAGTGGGTCC TGTTTTGCTT 
CGGTCTTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002301298.3
CDS	217..5568
Translation	MASSEADSRLSQVVSPALEKIVKNASWRKHSKLAHECKSVLEILTSRKPQQQHPPTSPSDDSSSESSLPGPLHDGGSIEYSLAESESILSPLINACNTQFLKIVDPAVDCIQKLIAHGYLRGEADSTGGTEAKLLAKLIESVCKCYDLGDDGAELLVLKTLLSAVTSISLRIHGDCLLQIVRTCYDIYLGSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPVEKTDVDGSMAVFVQGFITKIMQDIDGVFNPGTPSKSSMTVAHDGAFETTTGTVESTNPADLLDSTDKDMLDAKYWEISMYKTALEGRKGELADGEGEREDDLEVQIGNKLRRDAFLVFRALCKLSMKTPPKEALADPQLMRGKIVALELLKILLENAGAVFRTSDRFLGAIKQYLCLSLLKNSSSSLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRILENVVQPNFQQKIIVLRFLDKLCVDSQILVDIFINYDCDINSSNIFERMVNGLLKTAQGALPGTATTLVPPQEVTMKLEAMKSLVAILKSMGDWMNKQLRIPDPHSAKKSDAAENSPGPGSLPMTNGNGDEPVEGSDSHSETSTEASDVSAIEQRRAYKLEFQEGISLFNRKPKKGIEFLINANKVGNSAEEIAAFLKNASGLNKTLIGDYLGEREDFSLKVMHAYVDSFDFRGLEFDEAIRVFLQGFRLPGEAQKIDRIMEKFAERYCKCNPKVFSSADTAYVLAYSVILLNTDAHNPMVKNKMSADDFIRNNRGIDDGKDLPEEYLRSLFERISKNEIKMKEYDLALQQKQSLNSNRVLGLDSILNIVIRKRGEEKNMETSDDLIRHMQEQFKEKARKSESVYYAATDVVILRFMIEVCWAPMLAAFSVPLDQSDDEVVIALCLEGIRYAIHVTAVMSMKTHRDAFVTSLAKFTSLHSPADIKQKNIDAIKAIVTIADEDGNYLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNNSEKSKQSKSTILPVLKKKGPGRMQHAAASVLRGSYDSAGIGGNAAGAVTSEQMNNLVSNLNKLEQVGSSEMNRIFTRSQKLNSEAIIDFVKALCKVSVEELRSASDPRVFSLTKIVEIAHFNMNRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFMKPFVIVMRKSNAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTAAAYDDHKNIVLLAFEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNSRFNKDISLNAIAFLQFCATKLAEGDLGSSSRNKDKEVSVKISSPSPRTGKDGKQENGEIKDKEDHLYFWFPLLAGLSELSFDPRPEVRKSALQVLFETLRNHGHLFSLPLWERVFESVLFPIFDYVRHAIDPPGGNSPEQGIDGDMGELDQDAWLYGTCTLALQLVVDLFVKFYNTVNPLLRKVLSLLVSFIRRPHQSLAGIGIAAFVRLMSNAGDMFSEEKWLEVVLSLKDAANATLPDFSYIVSGESSVIADEQNNGETAGSDMPEDESEGLVTHHLYASISDAKCRAAVQLLLIQAVMEIYSMYRSQLSAKCALVLFDALHEVASHAHSINTNTTLRSKLQEFGSMTQMQDPPLLRLENESYQICLTFLQNLMMDRPPPFDEAEVESCLVNLCEEVLQFYVVTACSGQASETSTSGQCLWLIPLGSGKRRELAARAPLIVATLQAICSLGDSSFEKKLPHFFPLLSSLISCEHGSNEVQVALSDMLSSSVGPVLLRSC

Target ORF information:

RefSeq Version	XM_002301262.3
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa brefeldin A-inhibited guanine nucleotide-exchange protein 2 (LOC7494008), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002301262.3

ORF Insert Sequence:

ATGGCTTCCT CGGAAGCCGA TTCCCGGTTA AGCCAAGTGG TATCCCCTGC TTTAGAAAAA 
ATCGTCAAAA ACGCATCGTG GCGTAAACAC TCGAAATTAG CACACGAATG CAAATCCGTC 
CTCGAGATTC TCACTTCTCG AAAACCCCAA CAACAACATC CTCCGACTTC CCCGTCGGAC 
GACTCCTCAT CCGAATCCTC TCTCCCTGGC CCACTCCACG ACGGCGGGTC CATCGAGTAC 
TCCCTCGCCG AATCTGAATC TATCCTCAGC CCTCTGATCA ACGCGTGCAA CACTCAATTC 
CTCAAGATCG TCGATCCAGC CGTTGATTGC ATCCAGAAAT TGATCGCGCA TGGGTACCTA 
CGTGGCGAGG CGGACTCCAC CGGAGGTACT GAAGCGAAAT TGCTAGCTAA GTTGATTGAA 
TCTGTGTGTA AATGTTATGA TTTAGGGGAT GATGGTGCTG AATTGCTGGT TTTGAAAACC 
CTATTATCAG CCGTTACGTC GATTTCGTTG AGAATTCATG GAGATTGTTT GTTACAAATA 
GTGAGAACTT GTTATGACAT TTATTTAGGG AGTAAAAATG TAATTAATCA AACGACGGCG 
AAAGCGTCTT TGATTCAAAT GTTAGTTATT GTGTTTAGGA GAATGGAGGC TGATTCTTCA 
ACGGTTCCGG TTCAGCCGAT TGTTGTGGCT GAATTAATGG AACCAGTGGA GAAAACGGAT 
GTCGATGGAT CAATGGCGGT GTTTGTTCAA GGTTTTATAA CGAAAATTAT GCAGGATATA 
GATGGGGTTT TTAATCCGGG GACACCCAGT AAGTCTTCGA TGACAGTGGC ACATGATGGG 
GCTTTCGAGA CCACTACTGG TACGGTGGAG AGTACAAATC CGGCAGATTT GCTTGATTCG 
ACGGATAAGG ATATGTTAGA TGCCAAATAT TGGGAAATTA GTATGTATAA GACGGCTTTG 
GAAGGCAGGA AAGGGGAGTT GGCTGATGGG GAAGGGGAGA GGGAGGATGA TTTGGAAGTG 
CAGATTGGGA ATAAGTTGAG GAGAGATGCA TTTTTGGTGT TTCGAGCACT TTGTAAGTTA 
TCTATGAAAA CTCCGCCCAA AGAAGCATTG GCGGATCCAC AGTTGATGAG AGGGAAGATT 
GTTGCATTGG AGTTATTGAA GATTTTGTTG GAGAATGCTG GTGCTGTGTT TAGGACTAGT 
GACAGGTTCT TAGGTGCCAT CAAGCAATAT CTATGTCTTT CACTGTTGAA GAACAGCTCG 
TCAAGTCTTA TGATCATTTT CCAGCTTTCT TGCTCCATTT TCATTAGTTT GGTTTCAAGG 
TTCAGAGCTG GACTGAAAGC AGAAATTGGA GTATTTTTTC CTATGATTGT TCTCAGGATT 
CTTGAGAATG TTGTTCAACC TAATTTTCAG CAGAAGATTA TAGTACTTCG GTTTCTAGAT 
AAGCTCTGTG TTGATTCCCA GATCTTGGTA GACATTTTTA TTAATTATGA CTGTGATATC 
AACTCATCAA ACATATTTGA GAGAATGGTC AATGGACTTC TTAAAACTGC TCAAGGTGCC 
CTGCCTGGTA CTGCCACTAC ACTGGTGCCA CCTCAGGAGG TGACCATGAA ACTTGAAGCT 
ATGAAGTCCT TAGTGGCTAT TCTGAAATCA ATGGGAGACT GGATGAATAA ACAATTGCGC 
ATTCCCGATC CTCATTCTGC CAAGAAATCT GATGCAGCCG AAAACAGTCC TGGACCTGGA 
AGTCTTCCCA TGACAAATGG TAATGGGGAT GAGCCTGTTG AAGGATCAGA TTCTCATTCT 
GAAACCTCAA CTGAAGCATC GGATGTTTCA GCTATTGAGC AACGACGGGC TTACAAGCTG 
GAATTTCAGG AAGGTATATC TCTTTTCAAT CGGAAGCCTA AGAAAGGAAT TGAATTTCTA 
ATCAATGCAA ATAAAGTGGG GAATTCAGCA GAAGAGATAG CAGCCTTTCT TAAAAACGCA 
TCTGGCTTGA ACAAGACTCT GATTGGCGAT TACCTTGGGG AAAGAGAAGA TTTTTCACTG 
AAAGTAATGC ATGCTTATGT AGATTCTTTT GACTTTCGCG GCCTGGAGTT CGATGAGGCT 
ATCAGAGTCT TTCTACAGGG CTTTAGGTTA CCAGGTGAGG CACAAAAGAT TGATCGAATT 
ATGGAGAAGT TTGCTGAACG TTACTGCAAA TGTAACCCAA AGGTTTTTAG CAGTGCTGAC 
ACAGCCTATG TCCTTGCTTA CTCCGTGATA CTGCTCAACA CTGATGCTCA TAATCCCATG 
GTGAAGAATA AGATGTCAGC TGATGACTTC ATAAGAAACA ACCGTGGCAT TGATGATGGG 
AAAGATCTAC CTGAGGAGTA CTTGAGATCT TTGTTTGAAA GAATATCAAA AAATGAGATC 
AAAATGAAAG AATATGATTT AGCTCTCCAA CAAAAGCAGT CTTTGAATTC AAACAGAGTT 
TTAGGCTTGG ATAGTATCCT GAACATTGTG ATCCGCAAGC GGGGTGAAGA AAAGAATATG 
GAAACAAGTG ATGATCTTAT CAGGCACATG CAGGAACAAT TTAAAGAAAA AGCTCGAAAA 
TCTGAGTCAG TTTATTATGC TGCAACAGAT GTGGTGATTC TTAGATTCAT GATTGAGGTA 
TGCTGGGCTC CTATGCTGGC AGCCTTCAGT GTGCCTCTTG ACCAAAGTGA TGATGAAGTG 
GTGATAGCTT TGTGTCTTGA AGGTATCCGC TATGCAATCC ATGTTACTGC TGTGATGTCC 
ATGAAGACTC ATAGAGATGC TTTTGTGACT TCATTAGCAA AGTTTACATC GCTCCACTCC 
CCTGCTGATA TAAAGCAGAA AAATATTGAT GCTATCAAGG CAATAGTTAC AATTGCAGAT 
GAAGATGGGA ATTATTTGCA AGAAGCTTGG GAACATATTT TGACATGTGT CTCACGCTTT 
GAACATTTGC ATCTCCTGGG AGAGGGTGCT CCTCCAGATG CCACTTTTTT TGCTTTTCCC 
CAAAATAACT CTGAAAAGTC AAAACAAAGC AAGTCAACCA TCCTCCCTGT TCTGAAAAAG 
AAGGGACCCG GGAGGATGCA GCATGCAGCT GCTAGTGTGC TGAGAGGTTC ATATGATAGT 
GCTGGTATTG GTGGAAACGC TGCTGGGGCT GTCACAAGTG AGCAGATGAA CAATTTAGTT 
TCCAACTTAA ACAAGTTAGA GCAAGTTGGC AGCTCTGAGA TGAACCGCAT CTTCACACGG 
AGCCAAAAGT TAAACAGTGA GGCAATAATT GACTTTGTGA AGGCTCTTTG CAAGGTTTCT 
GTGGAGGAAT TGAGATCAGC TTCTGATCCA CGGGTCTTTA GCCTTACAAA GATTGTTGAG 
ATTGCGCACT TTAACATGAA TCGCATCAGG CTTGTATGGT CAAGCATCTG GCATGTGCTT 
TCTGATTTCT TTGTAACCAT TGGCTGTTCT GAAAACCTTT CCATTGCAAT TTTTGCAATG 
GACTCTTTAC GCCAATTATC AATGAAATTC TTAGACCGAG AAGAGTTGGC AAACTATAAT 
TTTCAGAATG AGTTTATGAA GCCATTTGTT ATTGTTATGC GGAAGAGTAA TGCTGTTGAA 
ATCAGAGAAT TGATTATCAG ATGTGTCTCG CAAATGGTCC TGTCTCGTGT CAATAATGTT 
AAATCTGGAT GGAAGAGCAT GTTCATGGTT TTCACAGCAG CAGCTTATGA TGACCACAAA 
AACATTGTAC TCTTGGCCTT TGAAATAATT GAAAAGATTA TTAGAGATTA CTTCCCATAC 
ATTACGGAGA CTGAAACAAC CACCTTTACT GATTGTGTGA ATTGCCTGAT AGCATTCACC 
AATAGTAGAT TCAACAAAGA CATTAGCCTT AATGCAATTG CTTTTCTTCA ATTCTGTGCC 
ACAAAACTTG CTGAAGGAGA TCTTGGATCT TCATCAAGGA ACAAAGACAA GGAAGTCTCT 
GTAAAAATTT CATCCCCTTC ACCTCGGACC GGAAAAGATG GAAAACAAGA GAATGGAGAA 
ATCAAAGACA AGGAAGATCA TCTTTATTTC TGGTTCCCTC TGTTGGCTGG TTTATCAGAA 
CTTAGCTTTG ACCCTAGGCC TGAAGTCAGG AAGAGTGCCT TGCAAGTTCT GTTTGAAACT 
TTACGCAATC ATGGTCATCT TTTTTCTCTA CCTTTATGGG AAAGAGTCTT TGAGTCGGTC 
TTATTTCCTA TATTTGACTA TGTGCGGCAT GCTATTGATC CTCCTGGAGG CAACTCCCCT 
GAGCAGGGGA TTGATGGTGA TATGGGTGAA CTTGATCAAG ATGCATGGCT CTATGGGACA 
TGTACCTTGG CTCTCCAACT GGTTGTAGAT CTTTTTGTGA AATTCTACAA TACTGTCAAT 
CCGCTTTTAA GAAAGGTGCT ATCGCTTCTG GTTAGCTTCA TTAGGCGTCC TCACCAAAGT 
CTTGCTGGTA TTGGTATTGC GGCATTTGTG CGTTTGATGA GCAATGCTGG AGATATGTTT 
TCAGAGGAGA AGTGGCTGGA AGTGGTTTTG TCATTGAAAG ATGCTGCCAA TGCAACACTC 
CCTGATTTCT CTTACATTGT AAGTGGAGAA TCATCGGTAA TAGCCGATGA ACAAAATAAT 
GGAGAGACTG CTGGATCTGA CATGCCCGAA GATGAATCAG AGGGACTGGT GACACACCAT 
CTTTATGCTT CTATATCTGA TGCAAAGTGT CGAGCTGCTG TTCAACTTCT GTTGATTCAG 
GCGGTGATGG AGATCTATAG CATGTACCGG TCTCAACTTT CAGCAAAATG TGCCTTGGTG 
CTCTTTGATG CTTTGCATGA GGTGGCATCC CATGCTCACA GTATCAACAC AAATACAACA 
TTACGTTCAA AACTACAAGA GTTTGGCTCT ATGACCCAAA TGCAAGACCC TCCACTATTA 
CGCCTGGAGA ATGAGTCCTA TCAAATTTGC CTCACATTCT TACAAAATCT TATGATGGAC 
AGACCTCCAC CTTTTGATGA AGCTGAAGTG GAATCCTGCC TTGTCAACCT CTGTGAAGAG 
GTTTTACAGT TTTACGTTGT AACTGCCTGT TCTGGACAGG CATCAGAAAC TTCTACCAGT 
GGACAATGCC TGTGGCTGAT TCCTTTAGGT TCTGGAAAAC GAAGGGAATT GGCTGCACGT 
GCACCTCTTA TTGTTGCAAC TCTCCAGGCC ATCTGTAGTT TGGGAGACTC TTCGTTTGAG 
AAGAAATTGC CTCATTTCTT CCCTCTTCTT TCGAGTTTGA TAAGTTGTGA GCATGGGTCA 
AATGAGGTCC AGGTAGCTCT CAGTGACATG CTCAGCTCAT CAGTGGGTCC TGTTTTGCTT 
CGGTCTTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)
Tuskan GA,Difazio S,Jansson S,Bohlmann J,Grigoriev I,Hellsten U,Putnam N,Ralph S,Rombauts S,Salamov A,Schein J,Sterck L,Aerts A,Bhalerao RR,Bhalerao RP,Blaudez D,Boerjan W,Brun A,Brunner A,Busov V,Campbell M,Carlson J,Chalot M,Chapman J,Chen GL,Cooper D,Coutinho PM,Couturier J,Covert S,Cronk Q,Cunningham R,Davis J,Degroeve S,Déjardin A,Depamphilis C,Detter J,Dirks B,Dubchak I,Duplessis S,Ehlting J,Ellis B,Gendler K,Goodstein D,Gribskov M,Grimwood J,Groover A,Gunter L,Hamberger B,Heinze B,Helariutta Y,Henrissat B,Holligan D,Holt R,Huang W,Islam-Faridi N,Jones S,Jones-Rhoades M,Jorgensen R,Joshi C,Kangasjärvi J,Karlsson J,Kelleher C,Kirkpatrick R,Kirst M,Kohler A,Kalluri U,Larimer F,Leebens-Mack J,Leplé JC,Locascio P,Lou Y,Lucas S,Martin F,Montanini B,Napoli C,Nelson DR,Nelson C,Nieminen K,Nilsson O,Pereda V,Peter G,Philippe R,Pilate G,Poliakov A,Razumovskaya J,Richardson P,Rinaldi C,Ritland K,Rouzé P,Ryaboy D,Schmutz J,Schrader J,Segerman B,Shin H,Siddiqui A,Sterky F,Terry A,Tsai CJ,Uberbacher E,Unneberg P,Vahala J,Wall K,Wessler S,Yang G,Yin T,Douglas C,Marra M,Sandberg G,Van de Peer Y,Rokhsar D

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POPTR_0002s15020g cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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