LOC7464867 cDNA ORF clone, Populus trichocarpa(black cottonwood)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC7464867
Entrez Gene ID	7464867
Full Name	guanine nucleotide exchange family protein
Synonyms	POPTRDRAFT_572117
Gene Type	protein-coding
Organism	Populus trichocarpa(black cottonwood)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002320028.2	XP_002320064.1	guanine nucleotide exchange family protein
XM_002320028.3	XP_002320064.1	brefeldin A-inhibited guanine nucleotide-exchange protein 2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, Déjardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasjärvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Leplé JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouzé P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC7464867 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC7464867 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPa29495	XM_002320028.3 Latest version!	Populus trichocarpa brefeldin A-inhibited guanine nucleotide-exchange protein 2 (LOC7464867), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OPa29495	XM_002320028.2	Populus trichocarpa guanine nucleotide exchange family protein (POPTR_0014s06630g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC7464867 ( XM_002320028.2 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_002320064.1 Populus trichocarpa guanine nucleotide exchange family protein (POPTR_0014s06630g) mRNA, complete cds.

Price & Availability↑

CloneID	OPa29495
Clone ID Related Accession (Same CDS sequence)	XM_002320028.2 , XM_002320028.3
Accession Version	XM_002320028.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1388332800000
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	guanine nucleotide exchange family protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008480). On Dec 16, 2013 this sequence version replaced XM_002320028.1. COMPLETENESS: incomplete on the 5' end.

ATGGCTTCCA CGGAAGCCGA TTCCCGGTTA AGCCAGGTAG TATCTCCTGC TTTAGAAAAA 
ATCATCAAAA ACGCATCGTG GCGTAAACAC TCGAAATTAG GACACGAATG CAAATCCGTC 
CTCGAAATTC TTACTTCACC AGAACCCCAA GAACAACCTC CTCCGACTTC CACTTCCGAC 
GACTCCTCCC CCTCCGAATC CTCTCTACCT GCCCCACTCC ACGACGGCGG GTCCCACGAA 
TACTCCCTTG CAGAATCCGA AACAATCCTC AGCCCTCTGA TCAACGCGTG CAACACTCAA 
TTCCTCAAGA TCGTCGATCC AGCCGTTGAT TGCATCCAGA AATTGATCGC CCATGGGTAC 
ATACGTGGAG AAGCGGATCC CACCGGAGGT GCAGAAGCGA AATTGTTAGC TAAATTGATC 
GAATCTGTGT GTAAATGTTA TGATTTAGGG GATGATGGTG TTGAATTGTT GGTTTTGAGA 
ACCCTATTAT CGGCTGTTAC GTCTATTTCC TTAAGGATTC ATGGAGATAG TTTGTTGCAA 
ATAGTGAGGA CTTGTTATGA TATTTATCTA GGGAGTAAAA ATGTGGTTAA TCAAACAACG 
GCGAAAGCAT CGTTGATTCA AATGTTAGTT ATTGTGTTTA GGAGAATGGA GGCTGATTCT 
TCGACAGTTC CGATTCAGCC GATTGTTGTG GCTGAATTAA TGGAACCAAT GGAGAAATCT 
GATGTGGATG GGTCAATGGC GGTGTTTGTT CAAGGTTTTA TAACCAAAAT TATGCAGGAT 
ATCGATGGGG TTTTAAATCC GGGGACACCG AGCAAGGCTT CAATGATGGG GGCACATGAT 
GGGGCTTTTG AGACTACGAC TAGTACAGTG GAGAGTACAA ATCCGGCTGA TTTGCTTGAT 
TCGACAGATA AGGATATGTT GGATGCGAAG TATTGGGAGA TTAGTATGTA CAAGACGGCT 
TTGGAGGGGA GGAAAGGGGA GTTGGCAGAT GGGGAAGGGG AAAGAGATGA TGATTTGGAA 
GTTCAGATTG GGAATAAGTT GAGGAGAGAT GCTTTTTTGG TGTTTCGAGC ACTTTGCAAG 
TTGTCTATGA AAACACCACC AAAAGAGGCT CTGACTGATC CTCAGTTGAT GAGAGGGAAG 
ATTGTCGCGT TGGAGTTATT GAAGATTTTG TTGGAGAATG CTGGTGCCGT GTTTAGGACT 
AGTGACAGGT TCTTAGGTGC CATCAAGCAA TATCTATGTC TTTCACTGTT GAAGAACAGC 
GCTTCGAGTC TAATGATCAT TTTCCAGCTT TCTTGCTCCA TTTTCATTAG TTTGGTTTCA 
AGATTTAGAG CTGGATTGAA AGCCGAGATT GGAGTATTTT TTCCTATGAT TGTTCTAAGG 
GTTCTGGAAA ATGTTGCTCA ACCTAATTAT CAGCAGAAGA TTATAGTGCT TCGGTTTCTA 
GATAAGCTCT GTGTTGATTC ACAGATCTTG GTAGACATTT TTATTAATTA TGATTGTGAT 
GTCAACTCAT CTAACATATT TGAGAGAATG GTCAATGGAC TCCTTAAAAC TGCTCAAGGC 
GCCCCTCCTG GTACTGCTAC TACACTTTTG CCACCTCAGG AGGTGTCCAT GAAACTTGAA 
GCTATGAAGT GCTTAGTGGG TATTCTGAAA TCAATGGGAG ACTGGATGAA TAAACAATTG 
CGCATTCCCG ATCCTCATTC TACCAAGAAA CCTGATGCAG CTGAGAACAG TCCTGAACCT 
GGAAGTCTTC CCATGGCAAA TGGTAATGGG GATGAGCCTG TTGACGGATC GGATTCTCAT 
TCTGAAACCT CCACTGAAGC ATCAGATGTT TCAACTATTG AGCAACGAAG GGCTTACAAG 
CTGGAACTTC AGGAAGGTAT ATCTCTTTTT AATCGGAAGC CTAAGAAAGG AATTGAGTTT 
CTAATCAATG CAAATAAAGT GGGCCATTCA GCAGAGGAGA TAGCAGCCTT TCTTAAAAAT 
GCATCGGGCT TGAACAAGAC TCTGATTGGT GATTACCTTG GGGAAAGAGA AGATTTGTCA 
CTGAAAGTGA TGCATGCTTA CGTGGATTCT TTTGACTTTC AAGACCTGGA GTTTGATGAG 
GCTATCAGGG TCTTTCTACA GGGATTTAGG TTACCAGGTG AGGCACAGAA GATTGATCGA 
ATTATGGAGA AGTTTGCTGA ACGTTACTGC AAATGTAATC CAAAAGTTTT TAGTAGTGCT 
GACACAGCCT ATGTCCTCGC TTACTCTGTG ATAATGCTCA ATACTGATGC TCATAATCCC 
ATGGTCAAAA GTAAGATGTC AGCTGATGAC TTCATTAGAA ACAATCGTGG CATTGACGAT 
GGGAAAGATC TACCTGAGGA GTTCTTGAGA TCATTGTTTG AACGAATATC AAAAAGTGAG 
ATCAAAATGA AAGAAGATAA TTTGGATCTC CAACAAAAGC AGTCTTTGAA TTCAAACAGA 
ATTTTAGGCT TGGACAGTAT CCTAAACATC GTGATCCGCA AGCGGGGCGA AGAAAAGCAT 
ATGGAAACAA GTGATGATCT TATCAGGCAC ATGCAGGAAC AATTTAAAGA AAAGGCTCGG 
AAATCTGAGT CAGTTTATTA TGCTGCAACA GATGTGGTGA TTCTTAGATT CATGGTTGAG 
GTATGCTGGG CTCCTATGTT GGCAGCCTTC AGTGTGCCTC TGGACCAAAG TGATGATGAA 
GTGGTGATAG CTCTGTGTCT TGAAGGTATC CGGTGTGCTA TTCATGTTAC TGCTGTGATG 
TCCATGAAGA CTCATAGAGA TGCTTTTGTG ACTTCATTAG CCAAGTTTAC TTCACTCCAT 
TCTCCTGCTG ATATCAAGCA GAAAAATATT GATGCCATCA AGGCAATAGT TACAATTGCA 
GATGAAGATG GGAATTATTT GCAAGAAGCT TGGGAACACA TTTTGACATG TGTCTCACGC 
TTCGAACATT TGCATCTCAT GGGAGAGGGT GCTCCTCCAG ATGCTACCTT TTTTGCTTTT 
CCCCAGAGTG ACTCAGAAAA GTCAAAACAA ACTAAGTCAA CCATCCTCCC TGTTCTGAAA 
AAGAAGGGAC CTGGAAGGAT GCAGTATGCA GCTGCTAGTG TGATGAGAGG TTCATATGAT 
AGTGCTGGTA TTGGTGGAAA CACAGCTGGG GCTGTTACTA GTGAGCAGAT GAACAATTTA 
GTCTCCAATT TAAACATGTT AGAACAAGTT GGAAGCTCTG AAATGAGTCG CATATTCACA 
CGGAGCCAAA AGTTAAACAG TGAGGCAATA ATTGACTTTG TCAAGGCTCT TTGCAAGGTC 
TCCATGGAGG AACTGCGATC AGCTTCTGAT CCACGGGTCT TTAGCCTTAC AAAGATTGTT 
GAGATTGCGC ACTATAACAT GAATCGTATC AGGCTTGTAT GGTCAAGCAT CTGGCACGTG 
CTTTCTGATT TCTTTGTAAC CATCGGCTGT TCTGAAAACC TTTCAATTGC AATTTTTGCA 
ATGGACTCTT TACGTCAATT ATCAATGAAA TTCTTAGAGC GGGAAGAGTT GGCAAACTAT 
AACTTTCAGA ATGAGTTTAT GAAGCCGTTT GTTATTGTTA TGCGGAAGAG TAATGCTGTT 
GAAATCAGAG AATTGATTAT CAGATGTGTC TCACAAATGG TTCTCTCTCG TGTCAATAAT 
GTTAAATCAG GATGGAAGAG CATGTTCATG GTTTTCACAA CAGCAGCTTA TGATGACCAC 
AAAAACATTG TACTCTTGGC CTTTGAAATA ATTGAAAAGA TTATTAGAGA TTACTTCCCA 
TACATCACGG AAACTGAAAC AACCACCTTT ACAGATTGTG TGAATTGCCT GATAGCATTC 
ACCAATAGTC GATTCAACAA AGACATTAGC CTTAATGCAA TTGCTTTTCT TCGATTTTGT 
GCCACAAAAC TTGCGGAAGG AGATCTTGGA TTTTCATCAA GGAACAAGGA CAAGGAAGCC 
CCTGGGAAAA TTTCAATACC TTCACCTCGG ACTGGAAAAG ATGGAAAACA AGAGAATGGA 
GAAATCACGG ATAGGGAAGA TCATCTCTAT TTCTGGTTCC CTTTGTTGGC TGGTTTATCA 
GAACTTAGCT TTGACCCTAG GCCTGAAATC AGGAAGAGTG CCTTGCAAAT TTTGTTTGAA 
ACTTTACGCA ATCATGGCCA CCTTTTTTCT CTACCTTTAT GGGAAAGAGT CTTTGAGTCG 
GTCTTATTTC CTATATTTGA CTATGTGCGG CATGCAATTG ATCCTACTGG AGGCGACGCC 
CCTGAGCAGG GGATTGATGG TGATACGGGT GAGCTTGATC AAGATGCGTG GCTCTATGAG 
ACATGCACCT TGGCTCTCCA ACTGGTTGTG GATCTTTTTG TAAAGTTCTA CAACACTGTC 
AATCCGCTTT TAAGAAAGGT GCTATTGCTT CTGGTTAGCT TCATTAGGCG TCCTCACCAA 
AGTCTTGCTG GTATTGGTAT TGCGGCATTT GTTCGTTTGA TGAGCAATGC TGGAGATCTT 
TTTTCAGAGG AGAAGTGGCT GGAGGTGGTT TTGTCATTAA AAGAGGCTGC CAATGCTACA 
CTCCCTGATT TCTCTTACAT TGTTAGTGGA GAAGCCTCGG TAATAAGCCA TGAACAAAGT 
GACGGAGAGA AGTCTGGTGA CATGCCTGAT GGTGATTCAG AGGGGCTGAT GGCGCACCGT 
CTTTATTCTT CTATATCTGA TGCAAAGTGT CGAGCTGCTG TTCAACTTCT ATTAATTCAG 
GCGGTGATGG AGATCTACAG CATGTATCGG TCTCACCTTT CAGCTAAAAG TGCCTTGGTG 
CTCTTTGATG CTTTGCATGA CGTGGCATCT CATGCTCACA GTATCAACAC CAATATTGCA 
TTACGTTCAA AGCTACTAGA GTTTGGCTCT ATGACCCAAA TGCAAGATCC TCCACTATTA 
CGCCTGGAGA ACGAGTCCTA TCAAATTTGC CTAACATTCC TACAAAATCT TATACTGGAC 
AGACCTCCAA CTTATGATGA AGCTCAAGTG GAATCCTGCC TTGTCAATCT CTGTGAGGAG 
GTTTTACAGT TTTACATTGC AACTGCCCAT GCTGGACAGA CATCAGAAAC TTCTCCCAGT 
GGCCAATCCC AGTGGCTGAT TCCTTTAGGT TCTGGAAAAC GAAGGGAATT GGCCACACGT 
GCTCCTCTTA TCGTTGCAAC TCTCCAGGCT ATCTGTAGTT TGGGAGATTC TTTGTTTGAG 
AAGAACTTGG CACATTTCTT CCCTCTTCTT TCAAGCTTGA TAAGTTGTGA ACATGGGTCA 
AATGAGGTCC AGGTTGCTCT CAGCGATATG CTCAGCTCAT CAGTGGGTCC TGTTTTGCTT 
CGGTCTTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002320064.1
CDS	1..5352
Translation	MASTEADSRLSQVVSPALEKIIKNASWRKHSKLGHECKSVLEILTSPEPQEQPPPTSTSDDSSPSESSLPAPLHDGGSHEYSLAESETILSPLINACNTQFLKIVDPAVDCIQKLIAHGYIRGEADPTGGAEAKLLAKLIESVCKCYDLGDDGVELLVLRTLLSAVTSISLRIHGDSLLQIVRTCYDIYLGSKNVVNQTTAKASLIQMLVIVFRRMEADSSTVPIQPIVVAELMEPMEKSDVDGSMAVFVQGFITKIMQDIDGVLNPGTPSKASMMGAHDGAFETTTSTVESTNPADLLDSTDKDMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEVQIGNKLRRDAFLVFRALCKLSMKTPPKEALTDPQLMRGKIVALELLKILLENAGAVFRTSDRFLGAIKQYLCLSLLKNSASSLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVAQPNYQQKIIVLRFLDKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGAPPGTATTLLPPQEVSMKLEAMKCLVGILKSMGDWMNKQLRIPDPHSTKKPDAAENSPEPGSLPMANGNGDEPVDGSDSHSETSTEASDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANKVGHSAEEIAAFLKNASGLNKTLIGDYLGEREDLSLKVMHAYVDSFDFQDLEFDEAIRVFLQGFRLPGEAQKIDRIMEKFAERYCKCNPKVFSSADTAYVLAYSVIMLNTDAHNPMVKSKMSADDFIRNNRGIDDGKDLPEEFLRSLFERISKSEIKMKEDNLDLQQKQSLNSNRILGLDSILNIVIRKRGEEKHMETSDDLIRHMQEQFKEKARKSESVYYAATDVVILRFMVEVCWAPMLAAFSVPLDQSDDEVVIALCLEGIRCAIHVTAVMSMKTHRDAFVTSLAKFTSLHSPADIKQKNIDAIKAIVTIADEDGNYLQEAWEHILTCVSRFEHLHLMGEGAPPDATFFAFPQSDSEKSKQTKSTILPVLKKKGPGRMQYAAASVMRGSYDSAGIGGNTAGAVTSEQMNNLVSNLNMLEQVGSSEMSRIFTRSQKLNSEAIIDFVKALCKVSMEELRSASDPRVFSLTKIVEIAHYNMNRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLEREELANYNFQNEFMKPFVIVMRKSNAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAAYDDHKNIVLLAFEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNSRFNKDISLNAIAFLRFCATKLAEGDLGFSSRNKDKEAPGKISIPSPRTGKDGKQENGEITDREDHLYFWFPLLAGLSELSFDPRPEIRKSALQILFETLRNHGHLFSLPLWERVFESVLFPIFDYVRHAIDPTGGDAPEQGIDGDTGELDQDAWLYETCTLALQLVVDLFVKFYNTVNPLLRKVLLLLVSFIRRPHQSLAGIGIAAFVRLMSNAGDLFSEEKWLEVVLSLKEAANATLPDFSYIVSGEASVISHEQSDGEKSGDMPDGDSEGLMAHRLYSSISDAKCRAAVQLLLIQAVMEIYSMYRSHLSAKSALVLFDALHDVASHAHSINTNIALRSKLLEFGSMTQMQDPPLLRLENESYQICLTFLQNLILDRPPTYDEAQVESCLVNLCEEVLQFYIATAHAGQTSETSPSGQSQWLIPLGSGKRRELATRAPLIVATLQAICSLGDSLFEKNLAHFFPLLSSLISCEHGSNEVQVALSDMLSSSVGPVLLRSC

Target ORF information:

RefSeq Version	XM_002320028.2
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa guanine nucleotide exchange family protein (POPTR_0014s06630g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002320028.2

ORF Insert Sequence:

ATGGCTTCCA CGGAAGCCGA TTCCCGGTTA AGCCAGGTAG TATCTCCTGC TTTAGAAAAA 
ATCATCAAAA ACGCATCGTG GCGTAAACAC TCGAAATTAG GACACGAATG CAAATCCGTC 
CTCGAAATTC TTACTTCACC AGAACCCCAA GAACAACCTC CTCCGACTTC CACTTCCGAC 
GACTCCTCCC CCTCCGAATC CTCTCTACCT GCCCCACTCC ACGACGGCGG GTCCCACGAA 
TACTCCCTTG CAGAATCCGA AACAATCCTC AGCCCTCTGA TCAACGCGTG CAACACTCAA 
TTCCTCAAGA TCGTCGATCC AGCCGTTGAT TGCATCCAGA AATTGATCGC CCATGGGTAC 
ATACGTGGAG AAGCGGATCC CACCGGAGGT GCAGAAGCGA AATTGTTAGC TAAATTGATC 
GAATCTGTGT GTAAATGTTA TGATTTAGGG GATGATGGTG TTGAATTGTT GGTTTTGAGA 
ACCCTATTAT CGGCTGTTAC GTCTATTTCC TTAAGGATTC ATGGAGATAG TTTGTTGCAA 
ATAGTGAGGA CTTGTTATGA TATTTATCTA GGGAGTAAAA ATGTGGTTAA TCAAACAACG 
GCGAAAGCAT CGTTGATTCA AATGTTAGTT ATTGTGTTTA GGAGAATGGA GGCTGATTCT 
TCGACAGTTC CGATTCAGCC GATTGTTGTG GCTGAATTAA TGGAACCAAT GGAGAAATCT 
GATGTGGATG GGTCAATGGC GGTGTTTGTT CAAGGTTTTA TAACCAAAAT TATGCAGGAT 
ATCGATGGGG TTTTAAATCC GGGGACACCG AGCAAGGCTT CAATGATGGG GGCACATGAT 
GGGGCTTTTG AGACTACGAC TAGTACAGTG GAGAGTACAA ATCCGGCTGA TTTGCTTGAT 
TCGACAGATA AGGATATGTT GGATGCGAAG TATTGGGAGA TTAGTATGTA CAAGACGGCT 
TTGGAGGGGA GGAAAGGGGA GTTGGCAGAT GGGGAAGGGG AAAGAGATGA TGATTTGGAA 
GTTCAGATTG GGAATAAGTT GAGGAGAGAT GCTTTTTTGG TGTTTCGAGC ACTTTGCAAG 
TTGTCTATGA AAACACCACC AAAAGAGGCT CTGACTGATC CTCAGTTGAT GAGAGGGAAG 
ATTGTCGCGT TGGAGTTATT GAAGATTTTG TTGGAGAATG CTGGTGCCGT GTTTAGGACT 
AGTGACAGGT TCTTAGGTGC CATCAAGCAA TATCTATGTC TTTCACTGTT GAAGAACAGC 
GCTTCGAGTC TAATGATCAT TTTCCAGCTT TCTTGCTCCA TTTTCATTAG TTTGGTTTCA 
AGATTTAGAG CTGGATTGAA AGCCGAGATT GGAGTATTTT TTCCTATGAT TGTTCTAAGG 
GTTCTGGAAA ATGTTGCTCA ACCTAATTAT CAGCAGAAGA TTATAGTGCT TCGGTTTCTA 
GATAAGCTCT GTGTTGATTC ACAGATCTTG GTAGACATTT TTATTAATTA TGATTGTGAT 
GTCAACTCAT CTAACATATT TGAGAGAATG GTCAATGGAC TCCTTAAAAC TGCTCAAGGC 
GCCCCTCCTG GTACTGCTAC TACACTTTTG CCACCTCAGG AGGTGTCCAT GAAACTTGAA 
GCTATGAAGT GCTTAGTGGG TATTCTGAAA TCAATGGGAG ACTGGATGAA TAAACAATTG 
CGCATTCCCG ATCCTCATTC TACCAAGAAA CCTGATGCAG CTGAGAACAG TCCTGAACCT 
GGAAGTCTTC CCATGGCAAA TGGTAATGGG GATGAGCCTG TTGACGGATC GGATTCTCAT 
TCTGAAACCT CCACTGAAGC ATCAGATGTT TCAACTATTG AGCAACGAAG GGCTTACAAG 
CTGGAACTTC AGGAAGGTAT ATCTCTTTTT AATCGGAAGC CTAAGAAAGG AATTGAGTTT 
CTAATCAATG CAAATAAAGT GGGCCATTCA GCAGAGGAGA TAGCAGCCTT TCTTAAAAAT 
GCATCGGGCT TGAACAAGAC TCTGATTGGT GATTACCTTG GGGAAAGAGA AGATTTGTCA 
CTGAAAGTGA TGCATGCTTA CGTGGATTCT TTTGACTTTC AAGACCTGGA GTTTGATGAG 
GCTATCAGGG TCTTTCTACA GGGATTTAGG TTACCAGGTG AGGCACAGAA GATTGATCGA 
ATTATGGAGA AGTTTGCTGA ACGTTACTGC AAATGTAATC CAAAAGTTTT TAGTAGTGCT 
GACACAGCCT ATGTCCTCGC TTACTCTGTG ATAATGCTCA ATACTGATGC TCATAATCCC 
ATGGTCAAAA GTAAGATGTC AGCTGATGAC TTCATTAGAA ACAATCGTGG CATTGACGAT 
GGGAAAGATC TACCTGAGGA GTTCTTGAGA TCATTGTTTG AACGAATATC AAAAAGTGAG 
ATCAAAATGA AAGAAGATAA TTTGGATCTC CAACAAAAGC AGTCTTTGAA TTCAAACAGA 
ATTTTAGGCT TGGACAGTAT CCTAAACATC GTGATCCGCA AGCGGGGCGA AGAAAAGCAT 
ATGGAAACAA GTGATGATCT TATCAGGCAC ATGCAGGAAC AATTTAAAGA AAAGGCTCGG 
AAATCTGAGT CAGTTTATTA TGCTGCAACA GATGTGGTGA TTCTTAGATT CATGGTTGAG 
GTATGCTGGG CTCCTATGTT GGCAGCCTTC AGTGTGCCTC TGGACCAAAG TGATGATGAA 
GTGGTGATAG CTCTGTGTCT TGAAGGTATC CGGTGTGCTA TTCATGTTAC TGCTGTGATG 
TCCATGAAGA CTCATAGAGA TGCTTTTGTG ACTTCATTAG CCAAGTTTAC TTCACTCCAT 
TCTCCTGCTG ATATCAAGCA GAAAAATATT GATGCCATCA AGGCAATAGT TACAATTGCA 
GATGAAGATG GGAATTATTT GCAAGAAGCT TGGGAACACA TTTTGACATG TGTCTCACGC 
TTCGAACATT TGCATCTCAT GGGAGAGGGT GCTCCTCCAG ATGCTACCTT TTTTGCTTTT 
CCCCAGAGTG ACTCAGAAAA GTCAAAACAA ACTAAGTCAA CCATCCTCCC TGTTCTGAAA 
AAGAAGGGAC CTGGAAGGAT GCAGTATGCA GCTGCTAGTG TGATGAGAGG TTCATATGAT 
AGTGCTGGTA TTGGTGGAAA CACAGCTGGG GCTGTTACTA GTGAGCAGAT GAACAATTTA 
GTCTCCAATT TAAACATGTT AGAACAAGTT GGAAGCTCTG AAATGAGTCG CATATTCACA 
CGGAGCCAAA AGTTAAACAG TGAGGCAATA ATTGACTTTG TCAAGGCTCT TTGCAAGGTC 
TCCATGGAGG AACTGCGATC AGCTTCTGAT CCACGGGTCT TTAGCCTTAC AAAGATTGTT 
GAGATTGCGC ACTATAACAT GAATCGTATC AGGCTTGTAT GGTCAAGCAT CTGGCACGTG 
CTTTCTGATT TCTTTGTAAC CATCGGCTGT TCTGAAAACC TTTCAATTGC AATTTTTGCA 
ATGGACTCTT TACGTCAATT ATCAATGAAA TTCTTAGAGC GGGAAGAGTT GGCAAACTAT 
AACTTTCAGA ATGAGTTTAT GAAGCCGTTT GTTATTGTTA TGCGGAAGAG TAATGCTGTT 
GAAATCAGAG AATTGATTAT CAGATGTGTC TCACAAATGG TTCTCTCTCG TGTCAATAAT 
GTTAAATCAG GATGGAAGAG CATGTTCATG GTTTTCACAA CAGCAGCTTA TGATGACCAC 
AAAAACATTG TACTCTTGGC CTTTGAAATA ATTGAAAAGA TTATTAGAGA TTACTTCCCA 
TACATCACGG AAACTGAAAC AACCACCTTT ACAGATTGTG TGAATTGCCT GATAGCATTC 
ACCAATAGTC GATTCAACAA AGACATTAGC CTTAATGCAA TTGCTTTTCT TCGATTTTGT 
GCCACAAAAC TTGCGGAAGG AGATCTTGGA TTTTCATCAA GGAACAAGGA CAAGGAAGCC 
CCTGGGAAAA TTTCAATACC TTCACCTCGG ACTGGAAAAG ATGGAAAACA AGAGAATGGA 
GAAATCACGG ATAGGGAAGA TCATCTCTAT TTCTGGTTCC CTTTGTTGGC TGGTTTATCA 
GAACTTAGCT TTGACCCTAG GCCTGAAATC AGGAAGAGTG CCTTGCAAAT TTTGTTTGAA 
ACTTTACGCA ATCATGGCCA CCTTTTTTCT CTACCTTTAT GGGAAAGAGT CTTTGAGTCG 
GTCTTATTTC CTATATTTGA CTATGTGCGG CATGCAATTG ATCCTACTGG AGGCGACGCC 
CCTGAGCAGG GGATTGATGG TGATACGGGT GAGCTTGATC AAGATGCGTG GCTCTATGAG 
ACATGCACCT TGGCTCTCCA ACTGGTTGTG GATCTTTTTG TAAAGTTCTA CAACACTGTC 
AATCCGCTTT TAAGAAAGGT GCTATTGCTT CTGGTTAGCT TCATTAGGCG TCCTCACCAA 
AGTCTTGCTG GTATTGGTAT TGCGGCATTT GTTCGTTTGA TGAGCAATGC TGGAGATCTT 
TTTTCAGAGG AGAAGTGGCT GGAGGTGGTT TTGTCATTAA AAGAGGCTGC CAATGCTACA 
CTCCCTGATT TCTCTTACAT TGTTAGTGGA GAAGCCTCGG TAATAAGCCA TGAACAAAGT 
GACGGAGAGA AGTCTGGTGA CATGCCTGAT GGTGATTCAG AGGGGCTGAT GGCGCACCGT 
CTTTATTCTT CTATATCTGA TGCAAAGTGT CGAGCTGCTG TTCAACTTCT ATTAATTCAG 
GCGGTGATGG AGATCTACAG CATGTATCGG TCTCACCTTT CAGCTAAAAG TGCCTTGGTG 
CTCTTTGATG CTTTGCATGA CGTGGCATCT CATGCTCACA GTATCAACAC CAATATTGCA 
TTACGTTCAA AGCTACTAGA GTTTGGCTCT ATGACCCAAA TGCAAGATCC TCCACTATTA 
CGCCTGGAGA ACGAGTCCTA TCAAATTTGC CTAACATTCC TACAAAATCT TATACTGGAC 
AGACCTCCAA CTTATGATGA AGCTCAAGTG GAATCCTGCC TTGTCAATCT CTGTGAGGAG 
GTTTTACAGT TTTACATTGC AACTGCCCAT GCTGGACAGA CATCAGAAAC TTCTCCCAGT 
GGCCAATCCC AGTGGCTGAT TCCTTTAGGT TCTGGAAAAC GAAGGGAATT GGCCACACGT 
GCTCCTCTTA TCGTTGCAAC TCTCCAGGCT ATCTGTAGTT TGGGAGATTC TTTGTTTGAG 
AAGAACTTGG CACATTTCTT CCCTCTTCTT TCAAGCTTGA TAAGTTGTGA ACATGGGTCA 
AATGAGGTCC AGGTTGCTCT CAGCGATATG CTCAGCTCAT CAGTGGGTCC TGTTTTGCTT 
CGGTCTTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7464867 ( XM_002320028.3 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_002320064.1 Populus trichocarpa brefeldin A-inhibited guanine nucleotide-exchange protein 2 (LOC7464867), mRNA.

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CloneID	OPa29495
Clone ID Related Accession (Same CDS sequence)	XM_002320028.2 , XM_002320028.3
Accession Version	XM_002320028.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037298.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 9, 2018 this sequence version replaced XM_002320028.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCCA CGGAAGCCGA TTCCCGGTTA AGCCAGGTAG TATCTCCTGC TTTAGAAAAA 
ATCATCAAAA ACGCATCGTG GCGTAAACAC TCGAAATTAG GACACGAATG CAAATCCGTC 
CTCGAAATTC TTACTTCACC AGAACCCCAA GAACAACCTC CTCCGACTTC CACTTCCGAC 
GACTCCTCCC CCTCCGAATC CTCTCTACCT GCCCCACTCC ACGACGGCGG GTCCCACGAA 
TACTCCCTTG CAGAATCCGA AACAATCCTC AGCCCTCTGA TCAACGCGTG CAACACTCAA 
TTCCTCAAGA TCGTCGATCC AGCCGTTGAT TGCATCCAGA AATTGATCGC CCATGGGTAC 
ATACGTGGAG AAGCGGATCC CACCGGAGGT GCAGAAGCGA AATTGTTAGC TAAATTGATC 
GAATCTGTGT GTAAATGTTA TGATTTAGGG GATGATGGTG TTGAATTGTT GGTTTTGAGA 
ACCCTATTAT CGGCTGTTAC GTCTATTTCC TTAAGGATTC ATGGAGATAG TTTGTTGCAA 
ATAGTGAGGA CTTGTTATGA TATTTATCTA GGGAGTAAAA ATGTGGTTAA TCAAACAACG 
GCGAAAGCAT CGTTGATTCA AATGTTAGTT ATTGTGTTTA GGAGAATGGA GGCTGATTCT 
TCGACAGTTC CGATTCAGCC GATTGTTGTG GCTGAATTAA TGGAACCAAT GGAGAAATCT 
GATGTGGATG GGTCAATGGC GGTGTTTGTT CAAGGTTTTA TAACCAAAAT TATGCAGGAT 
ATCGATGGGG TTTTAAATCC GGGGACACCG AGCAAGGCTT CAATGATGGG GGCACATGAT 
GGGGCTTTTG AGACTACGAC TAGTACAGTG GAGAGTACAA ATCCGGCTGA TTTGCTTGAT 
TCGACAGATA AGGATATGTT GGATGCGAAG TATTGGGAGA TTAGTATGTA CAAGACGGCT 
TTGGAGGGGA GGAAAGGGGA GTTGGCAGAT GGGGAAGGGG AAAGAGATGA TGATTTGGAA 
GTTCAGATTG GGAATAAGTT GAGGAGAGAT GCTTTTTTGG TGTTTCGAGC ACTTTGCAAG 
TTGTCTATGA AAACACCACC AAAAGAGGCT CTGACTGATC CTCAGTTGAT GAGAGGGAAG 
ATTGTCGCGT TGGAGTTATT GAAGATTTTG TTGGAGAATG CTGGTGCCGT GTTTAGGACT 
AGTGACAGGT TCTTAGGTGC CATCAAGCAA TATCTATGTC TTTCACTGTT GAAGAACAGC 
GCTTCGAGTC TAATGATCAT TTTCCAGCTT TCTTGCTCCA TTTTCATTAG TTTGGTTTCA 
AGATTTAGAG CTGGATTGAA AGCCGAGATT GGAGTATTTT TTCCTATGAT TGTTCTAAGG 
GTTCTGGAAA ATGTTGCTCA ACCTAATTAT CAGCAGAAGA TTATAGTGCT TCGGTTTCTA 
GATAAGCTCT GTGTTGATTC ACAGATCTTG GTAGACATTT TTATTAATTA TGATTGTGAT 
GTCAACTCAT CTAACATATT TGAGAGAATG GTCAATGGAC TCCTTAAAAC TGCTCAAGGC 
GCCCCTCCTG GTACTGCTAC TACACTTTTG CCACCTCAGG AGGTGTCCAT GAAACTTGAA 
GCTATGAAGT GCTTAGTGGG TATTCTGAAA TCAATGGGAG ACTGGATGAA TAAACAATTG 
CGCATTCCCG ATCCTCATTC TACCAAGAAA CCTGATGCAG CTGAGAACAG TCCTGAACCT 
GGAAGTCTTC CCATGGCAAA TGGTAATGGG GATGAGCCTG TTGACGGATC GGATTCTCAT 
TCTGAAACCT CCACTGAAGC ATCAGATGTT TCAACTATTG AGCAACGAAG GGCTTACAAG 
CTGGAACTTC AGGAAGGTAT ATCTCTTTTT AATCGGAAGC CTAAGAAAGG AATTGAGTTT 
CTAATCAATG CAAATAAAGT GGGCCATTCA GCAGAGGAGA TAGCAGCCTT TCTTAAAAAT 
GCATCGGGCT TGAACAAGAC TCTGATTGGT GATTACCTTG GGGAAAGAGA AGATTTGTCA 
CTGAAAGTGA TGCATGCTTA CGTGGATTCT TTTGACTTTC AAGACCTGGA GTTTGATGAG 
GCTATCAGGG TCTTTCTACA GGGATTTAGG TTACCAGGTG AGGCACAGAA GATTGATCGA 
ATTATGGAGA AGTTTGCTGA ACGTTACTGC AAATGTAATC CAAAAGTTTT TAGTAGTGCT 
GACACAGCCT ATGTCCTCGC TTACTCTGTG ATAATGCTCA ATACTGATGC TCATAATCCC 
ATGGTCAAAA GTAAGATGTC AGCTGATGAC TTCATTAGAA ACAATCGTGG CATTGACGAT 
GGGAAAGATC TACCTGAGGA GTTCTTGAGA TCATTGTTTG AACGAATATC AAAAAGTGAG 
ATCAAAATGA AAGAAGATAA TTTGGATCTC CAACAAAAGC AGTCTTTGAA TTCAAACAGA 
ATTTTAGGCT TGGACAGTAT CCTAAACATC GTGATCCGCA AGCGGGGCGA AGAAAAGCAT 
ATGGAAACAA GTGATGATCT TATCAGGCAC ATGCAGGAAC AATTTAAAGA AAAGGCTCGG 
AAATCTGAGT CAGTTTATTA TGCTGCAACA GATGTGGTGA TTCTTAGATT CATGGTTGAG 
GTATGCTGGG CTCCTATGTT GGCAGCCTTC AGTGTGCCTC TGGACCAAAG TGATGATGAA 
GTGGTGATAG CTCTGTGTCT TGAAGGTATC CGGTGTGCTA TTCATGTTAC TGCTGTGATG 
TCCATGAAGA CTCATAGAGA TGCTTTTGTG ACTTCATTAG CCAAGTTTAC TTCACTCCAT 
TCTCCTGCTG ATATCAAGCA GAAAAATATT GATGCCATCA AGGCAATAGT TACAATTGCA 
GATGAAGATG GGAATTATTT GCAAGAAGCT TGGGAACACA TTTTGACATG TGTCTCACGC 
TTCGAACATT TGCATCTCAT GGGAGAGGGT GCTCCTCCAG ATGCTACCTT TTTTGCTTTT 
CCCCAGAGTG ACTCAGAAAA GTCAAAACAA ACTAAGTCAA CCATCCTCCC TGTTCTGAAA 
AAGAAGGGAC CTGGAAGGAT GCAGTATGCA GCTGCTAGTG TGATGAGAGG TTCATATGAT 
AGTGCTGGTA TTGGTGGAAA CACAGCTGGG GCTGTTACTA GTGAGCAGAT GAACAATTTA 
GTCTCCAATT TAAACATGTT AGAACAAGTT GGAAGCTCTG AAATGAGTCG CATATTCACA 
CGGAGCCAAA AGTTAAACAG TGAGGCAATA ATTGACTTTG TCAAGGCTCT TTGCAAGGTC 
TCCATGGAGG AACTGCGATC AGCTTCTGAT CCACGGGTCT TTAGCCTTAC AAAGATTGTT 
GAGATTGCGC ACTATAACAT GAATCGTATC AGGCTTGTAT GGTCAAGCAT CTGGCACGTG 
CTTTCTGATT TCTTTGTAAC CATCGGCTGT TCTGAAAACC TTTCAATTGC AATTTTTGCA 
ATGGACTCTT TACGTCAATT ATCAATGAAA TTCTTAGAGC GGGAAGAGTT GGCAAACTAT 
AACTTTCAGA ATGAGTTTAT GAAGCCGTTT GTTATTGTTA TGCGGAAGAG TAATGCTGTT 
GAAATCAGAG AATTGATTAT CAGATGTGTC TCACAAATGG TTCTCTCTCG TGTCAATAAT 
GTTAAATCAG GATGGAAGAG CATGTTCATG GTTTTCACAA CAGCAGCTTA TGATGACCAC 
AAAAACATTG TACTCTTGGC CTTTGAAATA ATTGAAAAGA TTATTAGAGA TTACTTCCCA 
TACATCACGG AAACTGAAAC AACCACCTTT ACAGATTGTG TGAATTGCCT GATAGCATTC 
ACCAATAGTC GATTCAACAA AGACATTAGC CTTAATGCAA TTGCTTTTCT TCGATTTTGT 
GCCACAAAAC TTGCGGAAGG AGATCTTGGA TTTTCATCAA GGAACAAGGA CAAGGAAGCC 
CCTGGGAAAA TTTCAATACC TTCACCTCGG ACTGGAAAAG ATGGAAAACA AGAGAATGGA 
GAAATCACGG ATAGGGAAGA TCATCTCTAT TTCTGGTTCC CTTTGTTGGC TGGTTTATCA 
GAACTTAGCT TTGACCCTAG GCCTGAAATC AGGAAGAGTG CCTTGCAAAT TTTGTTTGAA 
ACTTTACGCA ATCATGGCCA CCTTTTTTCT CTACCTTTAT GGGAAAGAGT CTTTGAGTCG 
GTCTTATTTC CTATATTTGA CTATGTGCGG CATGCAATTG ATCCTACTGG AGGCGACGCC 
CCTGAGCAGG GGATTGATGG TGATACGGGT GAGCTTGATC AAGATGCGTG GCTCTATGAG 
ACATGCACCT TGGCTCTCCA ACTGGTTGTG GATCTTTTTG TAAAGTTCTA CAACACTGTC 
AATCCGCTTT TAAGAAAGGT GCTATTGCTT CTGGTTAGCT TCATTAGGCG TCCTCACCAA 
AGTCTTGCTG GTATTGGTAT TGCGGCATTT GTTCGTTTGA TGAGCAATGC TGGAGATCTT 
TTTTCAGAGG AGAAGTGGCT GGAGGTGGTT TTGTCATTAA AAGAGGCTGC CAATGCTACA 
CTCCCTGATT TCTCTTACAT TGTTAGTGGA GAAGCCTCGG TAATAAGCCA TGAACAAAGT 
GACGGAGAGA AGTCTGGTGA CATGCCTGAT GGTGATTCAG AGGGGCTGAT GGCGCACCGT 
CTTTATTCTT CTATATCTGA TGCAAAGTGT CGAGCTGCTG TTCAACTTCT ATTAATTCAG 
GCGGTGATGG AGATCTACAG CATGTATCGG TCTCACCTTT CAGCTAAAAG TGCCTTGGTG 
CTCTTTGATG CTTTGCATGA CGTGGCATCT CATGCTCACA GTATCAACAC CAATATTGCA 
TTACGTTCAA AGCTACTAGA GTTTGGCTCT ATGACCCAAA TGCAAGATCC TCCACTATTA 
CGCCTGGAGA ACGAGTCCTA TCAAATTTGC CTAACATTCC TACAAAATCT TATACTGGAC 
AGACCTCCAA CTTATGATGA AGCTCAAGTG GAATCCTGCC TTGTCAATCT CTGTGAGGAG 
GTTTTACAGT TTTACATTGC AACTGCCCAT GCTGGACAGA CATCAGAAAC TTCTCCCAGT 
GGCCAATCCC AGTGGCTGAT TCCTTTAGGT TCTGGAAAAC GAAGGGAATT GGCCACACGT 
GCTCCTCTTA TCGTTGCAAC TCTCCAGGCT ATCTGTAGTT TGGGAGATTC TTTGTTTGAG 
AAGAACTTGG CACATTTCTT CCCTCTTCTT TCAAGCTTGA TAAGTTGTGA ACATGGGTCA 
AATGAGGTCC AGGTTGCTCT CAGCGATATG CTCAGCTCAT CAGTGGGTCC TGTTTTGCTT 
CGGTCTTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002320064.1
CDS	213..5564
Translation	MASTEADSRLSQVVSPALEKIIKNASWRKHSKLGHECKSVLEILTSPEPQEQPPPTSTSDDSSPSESSLPAPLHDGGSHEYSLAESETILSPLINACNTQFLKIVDPAVDCIQKLIAHGYIRGEADPTGGAEAKLLAKLIESVCKCYDLGDDGVELLVLRTLLSAVTSISLRIHGDSLLQIVRTCYDIYLGSKNVVNQTTAKASLIQMLVIVFRRMEADSSTVPIQPIVVAELMEPMEKSDVDGSMAVFVQGFITKIMQDIDGVLNPGTPSKASMMGAHDGAFETTTSTVESTNPADLLDSTDKDMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEVQIGNKLRRDAFLVFRALCKLSMKTPPKEALTDPQLMRGKIVALELLKILLENAGAVFRTSDRFLGAIKQYLCLSLLKNSASSLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVAQPNYQQKIIVLRFLDKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGAPPGTATTLLPPQEVSMKLEAMKCLVGILKSMGDWMNKQLRIPDPHSTKKPDAAENSPEPGSLPMANGNGDEPVDGSDSHSETSTEASDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANKVGHSAEEIAAFLKNASGLNKTLIGDYLGEREDLSLKVMHAYVDSFDFQDLEFDEAIRVFLQGFRLPGEAQKIDRIMEKFAERYCKCNPKVFSSADTAYVLAYSVIMLNTDAHNPMVKSKMSADDFIRNNRGIDDGKDLPEEFLRSLFERISKSEIKMKEDNLDLQQKQSLNSNRILGLDSILNIVIRKRGEEKHMETSDDLIRHMQEQFKEKARKSESVYYAATDVVILRFMVEVCWAPMLAAFSVPLDQSDDEVVIALCLEGIRCAIHVTAVMSMKTHRDAFVTSLAKFTSLHSPADIKQKNIDAIKAIVTIADEDGNYLQEAWEHILTCVSRFEHLHLMGEGAPPDATFFAFPQSDSEKSKQTKSTILPVLKKKGPGRMQYAAASVMRGSYDSAGIGGNTAGAVTSEQMNNLVSNLNMLEQVGSSEMSRIFTRSQKLNSEAIIDFVKALCKVSMEELRSASDPRVFSLTKIVEIAHYNMNRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLEREELANYNFQNEFMKPFVIVMRKSNAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAAYDDHKNIVLLAFEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNSRFNKDISLNAIAFLRFCATKLAEGDLGFSSRNKDKEAPGKISIPSPRTGKDGKQENGEITDREDHLYFWFPLLAGLSELSFDPRPEIRKSALQILFETLRNHGHLFSLPLWERVFESVLFPIFDYVRHAIDPTGGDAPEQGIDGDTGELDQDAWLYETCTLALQLVVDLFVKFYNTVNPLLRKVLLLLVSFIRRPHQSLAGIGIAAFVRLMSNAGDLFSEEKWLEVVLSLKEAANATLPDFSYIVSGEASVISHEQSDGEKSGDMPDGDSEGLMAHRLYSSISDAKCRAAVQLLLIQAVMEIYSMYRSHLSAKSALVLFDALHDVASHAHSINTNIALRSKLLEFGSMTQMQDPPLLRLENESYQICLTFLQNLILDRPPTYDEAQVESCLVNLCEEVLQFYIATAHAGQTSETSPSGQSQWLIPLGSGKRRELATRAPLIVATLQAICSLGDSLFEKNLAHFFPLLSSLISCEHGSNEVQVALSDMLSSSVGPVLLRSC

Target ORF information:

RefSeq Version	XM_002320028.3
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa brefeldin A-inhibited guanine nucleotide-exchange protein 2 (LOC7464867), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002320028.3

ORF Insert Sequence:

ATGGCTTCCA CGGAAGCCGA TTCCCGGTTA AGCCAGGTAG TATCTCCTGC TTTAGAAAAA 
ATCATCAAAA ACGCATCGTG GCGTAAACAC TCGAAATTAG GACACGAATG CAAATCCGTC 
CTCGAAATTC TTACTTCACC AGAACCCCAA GAACAACCTC CTCCGACTTC CACTTCCGAC 
GACTCCTCCC CCTCCGAATC CTCTCTACCT GCCCCACTCC ACGACGGCGG GTCCCACGAA 
TACTCCCTTG CAGAATCCGA AACAATCCTC AGCCCTCTGA TCAACGCGTG CAACACTCAA 
TTCCTCAAGA TCGTCGATCC AGCCGTTGAT TGCATCCAGA AATTGATCGC CCATGGGTAC 
ATACGTGGAG AAGCGGATCC CACCGGAGGT GCAGAAGCGA AATTGTTAGC TAAATTGATC 
GAATCTGTGT GTAAATGTTA TGATTTAGGG GATGATGGTG TTGAATTGTT GGTTTTGAGA 
ACCCTATTAT CGGCTGTTAC GTCTATTTCC TTAAGGATTC ATGGAGATAG TTTGTTGCAA 
ATAGTGAGGA CTTGTTATGA TATTTATCTA GGGAGTAAAA ATGTGGTTAA TCAAACAACG 
GCGAAAGCAT CGTTGATTCA AATGTTAGTT ATTGTGTTTA GGAGAATGGA GGCTGATTCT 
TCGACAGTTC CGATTCAGCC GATTGTTGTG GCTGAATTAA TGGAACCAAT GGAGAAATCT 
GATGTGGATG GGTCAATGGC GGTGTTTGTT CAAGGTTTTA TAACCAAAAT TATGCAGGAT 
ATCGATGGGG TTTTAAATCC GGGGACACCG AGCAAGGCTT CAATGATGGG GGCACATGAT 
GGGGCTTTTG AGACTACGAC TAGTACAGTG GAGAGTACAA ATCCGGCTGA TTTGCTTGAT 
TCGACAGATA AGGATATGTT GGATGCGAAG TATTGGGAGA TTAGTATGTA CAAGACGGCT 
TTGGAGGGGA GGAAAGGGGA GTTGGCAGAT GGGGAAGGGG AAAGAGATGA TGATTTGGAA 
GTTCAGATTG GGAATAAGTT GAGGAGAGAT GCTTTTTTGG TGTTTCGAGC ACTTTGCAAG 
TTGTCTATGA AAACACCACC AAAAGAGGCT CTGACTGATC CTCAGTTGAT GAGAGGGAAG 
ATTGTCGCGT TGGAGTTATT GAAGATTTTG TTGGAGAATG CTGGTGCCGT GTTTAGGACT 
AGTGACAGGT TCTTAGGTGC CATCAAGCAA TATCTATGTC TTTCACTGTT GAAGAACAGC 
GCTTCGAGTC TAATGATCAT TTTCCAGCTT TCTTGCTCCA TTTTCATTAG TTTGGTTTCA 
AGATTTAGAG CTGGATTGAA AGCCGAGATT GGAGTATTTT TTCCTATGAT TGTTCTAAGG 
GTTCTGGAAA ATGTTGCTCA ACCTAATTAT CAGCAGAAGA TTATAGTGCT TCGGTTTCTA 
GATAAGCTCT GTGTTGATTC ACAGATCTTG GTAGACATTT TTATTAATTA TGATTGTGAT 
GTCAACTCAT CTAACATATT TGAGAGAATG GTCAATGGAC TCCTTAAAAC TGCTCAAGGC 
GCCCCTCCTG GTACTGCTAC TACACTTTTG CCACCTCAGG AGGTGTCCAT GAAACTTGAA 
GCTATGAAGT GCTTAGTGGG TATTCTGAAA TCAATGGGAG ACTGGATGAA TAAACAATTG 
CGCATTCCCG ATCCTCATTC TACCAAGAAA CCTGATGCAG CTGAGAACAG TCCTGAACCT 
GGAAGTCTTC CCATGGCAAA TGGTAATGGG GATGAGCCTG TTGACGGATC GGATTCTCAT 
TCTGAAACCT CCACTGAAGC ATCAGATGTT TCAACTATTG AGCAACGAAG GGCTTACAAG 
CTGGAACTTC AGGAAGGTAT ATCTCTTTTT AATCGGAAGC CTAAGAAAGG AATTGAGTTT 
CTAATCAATG CAAATAAAGT GGGCCATTCA GCAGAGGAGA TAGCAGCCTT TCTTAAAAAT 
GCATCGGGCT TGAACAAGAC TCTGATTGGT GATTACCTTG GGGAAAGAGA AGATTTGTCA 
CTGAAAGTGA TGCATGCTTA CGTGGATTCT TTTGACTTTC AAGACCTGGA GTTTGATGAG 
GCTATCAGGG TCTTTCTACA GGGATTTAGG TTACCAGGTG AGGCACAGAA GATTGATCGA 
ATTATGGAGA AGTTTGCTGA ACGTTACTGC AAATGTAATC CAAAAGTTTT TAGTAGTGCT 
GACACAGCCT ATGTCCTCGC TTACTCTGTG ATAATGCTCA ATACTGATGC TCATAATCCC 
ATGGTCAAAA GTAAGATGTC AGCTGATGAC TTCATTAGAA ACAATCGTGG CATTGACGAT 
GGGAAAGATC TACCTGAGGA GTTCTTGAGA TCATTGTTTG AACGAATATC AAAAAGTGAG 
ATCAAAATGA AAGAAGATAA TTTGGATCTC CAACAAAAGC AGTCTTTGAA TTCAAACAGA 
ATTTTAGGCT TGGACAGTAT CCTAAACATC GTGATCCGCA AGCGGGGCGA AGAAAAGCAT 
ATGGAAACAA GTGATGATCT TATCAGGCAC ATGCAGGAAC AATTTAAAGA AAAGGCTCGG 
AAATCTGAGT CAGTTTATTA TGCTGCAACA GATGTGGTGA TTCTTAGATT CATGGTTGAG 
GTATGCTGGG CTCCTATGTT GGCAGCCTTC AGTGTGCCTC TGGACCAAAG TGATGATGAA 
GTGGTGATAG CTCTGTGTCT TGAAGGTATC CGGTGTGCTA TTCATGTTAC TGCTGTGATG 
TCCATGAAGA CTCATAGAGA TGCTTTTGTG ACTTCATTAG CCAAGTTTAC TTCACTCCAT 
TCTCCTGCTG ATATCAAGCA GAAAAATATT GATGCCATCA AGGCAATAGT TACAATTGCA 
GATGAAGATG GGAATTATTT GCAAGAAGCT TGGGAACACA TTTTGACATG TGTCTCACGC 
TTCGAACATT TGCATCTCAT GGGAGAGGGT GCTCCTCCAG ATGCTACCTT TTTTGCTTTT 
CCCCAGAGTG ACTCAGAAAA GTCAAAACAA ACTAAGTCAA CCATCCTCCC TGTTCTGAAA 
AAGAAGGGAC CTGGAAGGAT GCAGTATGCA GCTGCTAGTG TGATGAGAGG TTCATATGAT 
AGTGCTGGTA TTGGTGGAAA CACAGCTGGG GCTGTTACTA GTGAGCAGAT GAACAATTTA 
GTCTCCAATT TAAACATGTT AGAACAAGTT GGAAGCTCTG AAATGAGTCG CATATTCACA 
CGGAGCCAAA AGTTAAACAG TGAGGCAATA ATTGACTTTG TCAAGGCTCT TTGCAAGGTC 
TCCATGGAGG AACTGCGATC AGCTTCTGAT CCACGGGTCT TTAGCCTTAC AAAGATTGTT 
GAGATTGCGC ACTATAACAT GAATCGTATC AGGCTTGTAT GGTCAAGCAT CTGGCACGTG 
CTTTCTGATT TCTTTGTAAC CATCGGCTGT TCTGAAAACC TTTCAATTGC AATTTTTGCA 
ATGGACTCTT TACGTCAATT ATCAATGAAA TTCTTAGAGC GGGAAGAGTT GGCAAACTAT 
AACTTTCAGA ATGAGTTTAT GAAGCCGTTT GTTATTGTTA TGCGGAAGAG TAATGCTGTT 
GAAATCAGAG AATTGATTAT CAGATGTGTC TCACAAATGG TTCTCTCTCG TGTCAATAAT 
GTTAAATCAG GATGGAAGAG CATGTTCATG GTTTTCACAA CAGCAGCTTA TGATGACCAC 
AAAAACATTG TACTCTTGGC CTTTGAAATA ATTGAAAAGA TTATTAGAGA TTACTTCCCA 
TACATCACGG AAACTGAAAC AACCACCTTT ACAGATTGTG TGAATTGCCT GATAGCATTC 
ACCAATAGTC GATTCAACAA AGACATTAGC CTTAATGCAA TTGCTTTTCT TCGATTTTGT 
GCCACAAAAC TTGCGGAAGG AGATCTTGGA TTTTCATCAA GGAACAAGGA CAAGGAAGCC 
CCTGGGAAAA TTTCAATACC TTCACCTCGG ACTGGAAAAG ATGGAAAACA AGAGAATGGA 
GAAATCACGG ATAGGGAAGA TCATCTCTAT TTCTGGTTCC CTTTGTTGGC TGGTTTATCA 
GAACTTAGCT TTGACCCTAG GCCTGAAATC AGGAAGAGTG CCTTGCAAAT TTTGTTTGAA 
ACTTTACGCA ATCATGGCCA CCTTTTTTCT CTACCTTTAT GGGAAAGAGT CTTTGAGTCG 
GTCTTATTTC CTATATTTGA CTATGTGCGG CATGCAATTG ATCCTACTGG AGGCGACGCC 
CCTGAGCAGG GGATTGATGG TGATACGGGT GAGCTTGATC AAGATGCGTG GCTCTATGAG 
ACATGCACCT TGGCTCTCCA ACTGGTTGTG GATCTTTTTG TAAAGTTCTA CAACACTGTC 
AATCCGCTTT TAAGAAAGGT GCTATTGCTT CTGGTTAGCT TCATTAGGCG TCCTCACCAA 
AGTCTTGCTG GTATTGGTAT TGCGGCATTT GTTCGTTTGA TGAGCAATGC TGGAGATCTT 
TTTTCAGAGG AGAAGTGGCT GGAGGTGGTT TTGTCATTAA AAGAGGCTGC CAATGCTACA 
CTCCCTGATT TCTCTTACAT TGTTAGTGGA GAAGCCTCGG TAATAAGCCA TGAACAAAGT 
GACGGAGAGA AGTCTGGTGA CATGCCTGAT GGTGATTCAG AGGGGCTGAT GGCGCACCGT 
CTTTATTCTT CTATATCTGA TGCAAAGTGT CGAGCTGCTG TTCAACTTCT ATTAATTCAG 
GCGGTGATGG AGATCTACAG CATGTATCGG TCTCACCTTT CAGCTAAAAG TGCCTTGGTG 
CTCTTTGATG CTTTGCATGA CGTGGCATCT CATGCTCACA GTATCAACAC CAATATTGCA 
TTACGTTCAA AGCTACTAGA GTTTGGCTCT ATGACCCAAA TGCAAGATCC TCCACTATTA 
CGCCTGGAGA ACGAGTCCTA TCAAATTTGC CTAACATTCC TACAAAATCT TATACTGGAC 
AGACCTCCAA CTTATGATGA AGCTCAAGTG GAATCCTGCC TTGTCAATCT CTGTGAGGAG 
GTTTTACAGT TTTACATTGC AACTGCCCAT GCTGGACAGA CATCAGAAAC TTCTCCCAGT 
GGCCAATCCC AGTGGCTGAT TCCTTTAGGT TCTGGAAAAC GAAGGGAATT GGCCACACGT 
GCTCCTCTTA TCGTTGCAAC TCTCCAGGCT ATCTGTAGTT TGGGAGATTC TTTGTTTGAG 
AAGAACTTGG CACATTTCTT CCCTCTTCTT TCAAGCTTGA TAAGTTGTGA ACATGGGTCA 
AATGAGGTCC AGGTTGCTCT CAGCGATATG CTCAGCTCAT CAGTGGGTCC TGTTTTGCTT 
CGGTCTTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)
Tuskan GA,Difazio S,Jansson S,Bohlmann J,Grigoriev I,Hellsten U,Putnam N,Ralph S,Rombauts S,Salamov A,Schein J,Sterck L,Aerts A,Bhalerao RR,Bhalerao RP,Blaudez D,Boerjan W,Brun A,Brunner A,Busov V,Campbell M,Carlson J,Chalot M,Chapman J,Chen GL,Cooper D,Coutinho PM,Couturier J,Covert S,Cronk Q,Cunningham R,Davis J,Degroeve S,Déjardin A,Depamphilis C,Detter J,Dirks B,Dubchak I,Duplessis S,Ehlting J,Ellis B,Gendler K,Goodstein D,Gribskov M,Grimwood J,Groover A,Gunter L,Hamberger B,Heinze B,Helariutta Y,Henrissat B,Holligan D,Holt R,Huang W,Islam-Faridi N,Jones S,Jones-Rhoades M,Jorgensen R,Joshi C,Kangasjärvi J,Karlsson J,Kelleher C,Kirkpatrick R,Kirst M,Kohler A,Kalluri U,Larimer F,Leebens-Mack J,Leplé JC,Locascio P,Lou Y,Lucas S,Martin F,Montanini B,Napoli C,Nelson DR,Nelson C,Nieminen K,Nilsson O,Pereda V,Peter G,Philippe R,Pilate G,Poliakov A,Razumovskaya J,Richardson P,Rinaldi C,Ritland K,Rouzé P,Ryaboy D,Schmutz J,Schrader J,Segerman B,Shin H,Siddiqui A,Sterky F,Terry A,Tsai CJ,Uberbacher E,Unneberg P,Vahala J,Wall K,Wessler S,Yang G,Yin T,Douglas C,Marra M,Sandberg G,Van de Peer Y,Rokhsar D

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LOC7464867 cDNA ORF clone, Populus trichocarpa(black cottonwood)

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