LOC7459153 cDNA ORF clone, Populus trichocarpa(black cottonwood)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC7459153
Entrez Gene ID	7459153
Full Name	hypothetical protein
Synonyms	POPTRDRAFT_642431
Gene Type	protein-coding
Organism	Populus trichocarpa(black cottonwood)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002300431.2	XP_002300467.2	hypothetical protein
XM_024585974.1	XP_024441742.1	probable disease resistance protein At5g63020 isoform X1
XM_024585987.1	XP_024441755.1	disease resistance protein At4g27190 isoform X2
XM_024585969.1	XP_024441737.1	probable disease resistance protein At5g63020 isoform X1
XM_024585979.1	XP_024441747.1	probable disease resistance protein At5g63020 isoform X1
XM_024585984.1	XP_024441752.1	probable disease resistance protein At5g63020 isoform X1
XM_024585992.1	XP_024441760.1	disease resistance protein At4g27190 isoform X3
XM_024585964.1	XP_024441732.1	probable disease resistance protein At5g63020 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, Déjardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasjärvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Leplé JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouzé P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC7459153 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC7459153 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPa422386	XM_024585979.1 Latest version!	Populus trichocarpa probable disease resistance protein At5g63020 (LOC7459153), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPa422386	XM_024585984.1 Latest version!	Populus trichocarpa probable disease resistance protein At5g63020 (LOC7459153), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPa422386	XM_024585969.1 Latest version!	Populus trichocarpa probable disease resistance protein At5g63020 (LOC7459153), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPa422386	XM_024585964.1 Latest version!	Populus trichocarpa probable disease resistance protein At5g63020 (LOC7459153), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPa422386	XM_024585974.1 Latest version!	Populus trichocarpa probable disease resistance protein At5g63020 (LOC7459153), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPa422387	XM_024585987.1 Latest version!	Populus trichocarpa probable disease resistance protein At5g63020 (LOC7459153), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPa422388	XM_024585992.1 Latest version!	Populus trichocarpa probable disease resistance protein At5g63020 (LOC7459153), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC7459153 ( XM_024585974.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024441742.1 Populus trichocarpa probable disease resistance protein At5g63020 (LOC7459153), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPa422386
Clone ID Related Accession (Same CDS sequence)	XM_024585974.1 , XM_024585969.1 , XM_024585979.1 , XM_024585984.1 , XM_024585964.1
Accession Version	XM_024585974.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	probable disease resistance protein At5g63020 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTTG AAAGTGCTGG TGGATCCATT ATAGCTATGC TAGCAGAACT CATGGTGGAA 
CCAGTAGGAA GGCTGTTCCG TTACATGTTC TGTTTCAACA ATTTTGTTGA AGAATTCAAA 
GAACGAAAGG AGAACCTTGC TTTAGCACTA GATGGTCTGC AAAAAGAAGT CGAAGCTGCT 
GAAAGGAATG CTGAAGAAAT TAAGAAGGAT GTCAAAAAGT GGCTGGAAGA TGCAAAAAAC 
GAAATTGAAG GTGCGAAGCC ATTGGAAAAT GAAATAGGAA AAAATGGCAA ATGCTTTACT 
TGGTGCCCAA ACTGCATGCG ACAATTCACG TTAAGCAAGG CACTGGCCAA GAAGTCGGAG 
ACTTTCACAA AACTTGGAGA AACAAAGTTT ACAAAAGTGG CCGACGAACG TCCTCCTCAG 
CGCATAGAAT TTCTAACATC AAAGGAATTC ACGCCCTCAA AATCGTCAGA AGAAGCTTTG 
GAACAGATTA TGGAAGCTCT CAAAGATGAC AACGTCAATA TGATCGGACT GTACGGCATG 
GGAGGGGTGG GTAAAACCAC CTTGGTGAAA GAAGTAGGCA GGAGAGCAAA AGAGTTGCAG 
CTTTTTCCTG AAGTTTTGAT GGCTACGGTG TCCCAGAATC AAAATGTTAC AGACATCCAG 
GATCGAATGG CAGATAAGTT ATGTCTGGAT ATTAAAGAAA AGAGTAAAGA AGGGAGAGCA 
GATCGATTAT GGCAGAGACT GAAGCAAGTG GAGAAGATGC TTATAATCCT GGATGATGTT 
TGGAAACATA TTGACTTGAA AGAGATAGGG ATCCCATTTG GTGATGATCA CAGGGGTTGT 
AAAATTCTTC TAACAACACG TCGTCGAGAC ATATGTTCTT ATATGGTGTG CCAGAAAAAT 
GTGTTTCTAC GTCTCTTTTC TGAAAAAGAA GCATGGGATT TATTCAGAAT CAATGCAGGT 
TTAGATGATG GGGACTCTAC CTTGAACAGA GTGGCAAGGG ATGTTGCGAG AGAATGCCAC 
GGCTTGCCTA TAGCACTTGT GACAATGGGA AGGGCTCTAA GAGATGAATC TGCAGTTAAG 
TGGAAAAGAG TGTCTAAACA GCTCAAAAAC TCTCAATTTC CGGACATGGA ACAAATTGAA 
GAAAAAAATG CATATGCATG TCTTAAGTTG AGCTACGATT ATTTGAAGTC CAAGGAAACC 
AAGTTATGTT TCTTGCTATG CTGTTTATTT CCAGAAGATT ACAACATTCC AGTCGAGGAC 
TTGACGAGAT ATGCAGTTGG CTATGGGTTA CATCAAGATG GGGAGCCCAT TGAAGATGCA 
AGGGAACAAG TTCATGTGGC AATCCAAGAC CTCAAATCTT GCTGTTTGTT GTTGGGCACT 
GAAACTGAAG AACATGTGAG AATGCATGAC TTGGTTCGTG ATGTTGCTAT TCAGATAGCA 
TCATCAAAAG AATATGGATT CATGGTATTG GAGAAGTGGC CATTGAGTAT TGAAAGCTTT 
GAAGCTTGTA CAACAATTTC GTTAATGGGC AATAAACTAA CCGAACTTCC TGAAGGATTG 
GAATGTCCAC AGCTCAAAGT TCTATTGTTA GAATTGGATT ATGGTTTGTA TGTTCCTCAG 
AGGTTCTTTG AAGGGATGAA AGAAATTGAA GTTTTGTCTC TGGAGGGAGG GTGTTTGTCA 
TTGCAATCAC TTGAACTCTC AACGAAACTT CAATCGTTGG TGTTGATCAG GTGTGAATGC 
AAGGACCTCA TTTGGTTGAG AAAGCTGCAG AGACTTAAGA TTCTTGTTTT TATACGGTGC 
TTATCCATTG AAGAATTACC TGATGAAATA GGAGAGCTCA AGGAGTTAAG GTTGTTGGAT 
GTGACAGGTT GTCAAAGGCT AAGAAGGATC CCTGTGAATT TGATTGGAAG GTTGAAGAAG 
TTAGAAGAGC TGTTGATCGG GCATCGCAGC TTTGATGGAT GGGATGTTGT TGGATGTGAC 
AGCACCGGAG GAATGAATGC AAGCCTGACA GAACTAAATT CGTTGTCTCA GTTAGCCGTA 
TTATCATTGA GGATCCCGAA GGTTGAATGC ACTCCCAGAG ATTTTGTTTT TCCAGTTAGC 
TTGCGCAAAT ATGATATAAT ATTAGGGAAT CCGTTTGTGG CAGCCAATTA TGGATACCCA 
ACCTCGACAA GATTAATTTT GGCTGGTACA TCCTTAAATG CGAAGACATT TGAGCAATTG 
TTTTTACATA AATTAGAATT TGTAGAAGTG ACGCATTGTG GGGATGTTTT CACTCTGTTT 
CCAGCAAGAT TGCGGCAAGG TTTAAAAAAT CTGAAGGAGG TGATTGTTGA CAGCTGTAAA 
TCATTGGAAG AGGTGTTTGA ATTGGGTGAG GCTGATGAAG GAAGCAGTGA GGAGAAGGAG 
CTGCCGCTGC TGTCATCTTT AACAGAGTTA AAGCTGCGAT GGTTACCTGA GCTCAAATGC 
ATATGGCCCA CCGGACATAT CAGCCTCCAA AATCTTGCTC ATCTGGAGTT GTTTTCTCTC 
CACAAACTGA CATTTATCTT CACACCGCCC CTCGCTCGAA GTTTGTCAAA GCTAGAAGGC 
CTTTACATAA GAAATTGCGG TGAATTGAAG CATATTATCA GAGAAGAGGA AGGTGAAAGG 
GAAATAATTC CAGAGTCTCC TTGCTTCCCA GAATTAAAAA ATATCAACAT ATATTTCTGT 
GATAAACTGG AATATGTCTT GCCAGTCTCT TTATCTCATG ACAGAGATGG CATCATCAAG 
TTCCCTCAAC TAAGAGAATT GTCTCTTAAG CTCGGATCAA ATTACAGCTT TTTAGGTCCA 
AGGAATTTTG ATGCTCAATT GCCTTTGCAA GAATTAACCA TTAAAGGCCA CGAAGAAGTG 
GGTAATTGGT TGGCACAGCT ACAACAAAAC GGCTTCTTAC AAAGATTAGA ATATGTACAC 
GTGGACGATT GTGCGGATGT TCGCACTCCG TTTCCAGCAA AATTGCTGCG AGCTTTGAAA 
AATCTAAGGA TGGTGAACAT ATACGGCTGC AAATCATTGG AAGAGGTATT TGAATTAGGT 
GAGGCTGATG AAGGAAGCAG TGAGGAGAAG GAGCTGCCGC TGCTGTCATC TTTAACAGAG 
TTACAGCTGC AAAGGTTACC TGAGCTCAAA TGCATATGGA AGGGGCCCAC CAGAAATGTC 
AGCCTCCAAA GTCTTGTTCA TCTGAATGTT TGGTGTCTCA ACAAACTGAC ATTTATCTTC 
ACATCATCCC TCGCGCAGAG TCTGCCAAAG CTAGAAAGAC TTTCCATGAG TGATTGCGGT 
GAATTGAAGC ATATTATCAG AGAAGAGGAT GGTGAAAGGG AAATAATTCC AGAGTCTCCT 
GGCTTCCCAA AATTAAAAAC TATCAGCATA TCTGACTGTG GTAAACTGGA ATATGTCCTC 
CCTGTCTCTG TGTCTCCAAG CCTTCTGAAC CTGAACGAGA TGCGGATTTT TAAAGCTCAT 
AATTTAAAGC AAATATTTTA CAGTGGAGAA GGAGATGCAC TCACCAGAGA TGGCATCATC 
AAGTTCCCTA AGCTAAGAGA ATTGTATCTT TCAAATTGCA GCTTCTTTGG TCCAAAGAAT 
TTTACTGCCC AATTGCCTTC CTTGCAAATT CTAGAAATTG ATGGCCACAA AGAATTGGGA 
AATTTGTTTG CACAGCTCCA AGGGTTGACA AATTTGGAAA CATTACGCTT GCGCTCCCTG 
CTGGTGCCTG ATATTAGGTG TCTATGGAAG GGTCTCGTGT TGAGCAAATT GACTACTTTG 
AATGTGGTTG AGTGTAAAAG ACTGACGCAT GTATTCACAC GCAGTATGAT TGTCAGTCTA 
GTTCCACTGA AAGTTCTAAA GATATTGTCC TGTGAGGAAT TGGAGCAAAT CATTGCTAAG 
GATGATGATG AAAACGACCA GATATTGTTA GGAGATCATC TCCAATCTTT ATGCTTTCCC 
AATTTGTGTG AAATTGAGAT TAGAGAATGC AACAAGTTGA AGAGTCTCTT CCCATTAGCC 
ATGGCTTCAG GTCTCCCAAA TCTCCAAATA CTGAGGGTAA CAAAAGCCTC TCAATTATTG 
GGAGTCTTTG GGCAGGATGA TCATGCTTCA CCTGTCAACG TTGAGAAGGA GATGGTGCTT 
CCTAATCTAA AAAAGTTGTC TCTAGAACAA TTATCAAGTA TTGTCTGCTT CAGTTTCGGA 
TGCTGTGATT TCTTATTCCC TCGTTTGGAG AAGTTGAAGG TGTATGAATG CTCAAAGTTG 
ACCATAAAAT TTGCTATTAC ACCAAATGGT GGTTCAATGA GTGCTCAATC AGAGGTATCT 
CAACCAGCTG AAGATTCAAG CACTAATAGA GAGTGGACCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024441742.1
CDS	420..4784
Translation	MALESAGGSIIAMLAELMVEPVGRLFRYMFCFNNFVEEFKERKENLALALDGLQKEVEAAERNAEEIKKDVKKWLEDAKNEIEGAKPLENEIGKNGKCFTWCPNCMRQFTLSKALAKKSETFTKLGETKFTKVADERPPQRIEFLTSKEFTPSKSSEEALEQIMEALKDDNVNMIGLYGMGGVGKTTLVKEVGRRAKELQLFPEVLMATVSQNQNVTDIQDRMADKLCLDIKEKSKEGRADRLWQRLKQVEKMLIILDDVWKHIDLKEIGIPFGDDHRGCKILLTTRRRDICSYMVCQKNVFLRLFSEKEAWDLFRINAGLDDGDSTLNRVARDVARECHGLPIALVTMGRALRDESAVKWKRVSKQLKNSQFPDMEQIEEKNAYACLKLSYDYLKSKETKLCFLLCCLFPEDYNIPVEDLTRYAVGYGLHQDGEPIEDAREQVHVAIQDLKSCCLLLGTETEEHVRMHDLVRDVAIQIASSKEYGFMVLEKWPLSIESFEACTTISLMGNKLTELPEGLECPQLKVLLLELDYGLYVPQRFFEGMKEIEVLSLEGGCLSLQSLELSTKLQSLVLIRCECKDLIWLRKLQRLKILVFIRCLSIEELPDEIGELKELRLLDVTGCQRLRRIPVNLIGRLKKLEELLIGHRSFDGWDVVGCDSTGGMNASLTELNSLSQLAVLSLRIPKVECTPRDFVFPVSLRKYDIILGNPFVAANYGYPTSTRLILAGTSLNAKTFEQLFLHKLEFVEVTHCGDVFTLFPARLRQGLKNLKEVIVDSCKSLEEVFELGEADEGSSEEKELPLLSSLTELKLRWLPELKCIWPTGHISLQNLAHLELFSLHKLTFIFTPPLARSLSKLEGLYIRNCGELKHIIREEEGEREIIPESPCFPELKNINIYFCDKLEYVLPVSLSHDRDGIIKFPQLRELSLKLGSNYSFLGPRNFDAQLPLQELTIKGHEEVGNWLAQLQQNGFLQRLEYVHVDDCADVRTPFPAKLLRALKNLRMVNIYGCKSLEEVFELGEADEGSSEEKELPLLSSLTELQLQRLPELKCIWKGPTRNVSLQSLVHLNVWCLNKLTFIFTSSLAQSLPKLERLSMSDCGELKHIIREEDGEREIIPESPGFPKLKTISISDCGKLEYVLPVSVSPSLLNLNEMRIFKAHNLKQIFYSGEGDALTRDGIIKFPKLRELYLSNCSFFGPKNFTAQLPSLQILEIDGHKELGNLFAQLQGLTNLETLRLRSLLVPDIRCLWKGLVLSKLTTLNVVECKRLTHVFTRSMIVSLVPLKVLKILSCEELEQIIAKDDDENDQILLGDHLQSLCFPNLCEIEIRECNKLKSLFPLAMASGLPNLQILRVTKASQLLGVFGQDDHASPVNVEKEMVLPNLKKLSLEQLSSIVCFSFGCCDFLFPRLEKLKVYECSKLTIKFAITPNGGSMSAQSEVSQPAEDSSTNREWTS

Target ORF information:

RefSeq Version	XM_024585974.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa probable disease resistance protein At5g63020 (LOC7459153), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024585974.1

ORF Insert Sequence:

ATGGCTCTTG AAAGTGCTGG TGGATCCATT ATAGCTATGC TAGCAGAACT CATGGTGGAA 
CCAGTAGGAA GGCTGTTCCG TTACATGTTC TGTTTCAACA ATTTTGTTGA AGAATTCAAA 
GAACGAAAGG AGAACCTTGC TTTAGCACTA GATGGTCTGC AAAAAGAAGT CGAAGCTGCT 
GAAAGGAATG CTGAAGAAAT TAAGAAGGAT GTCAAAAAGT GGCTGGAAGA TGCAAAAAAC 
GAAATTGAAG GTGCGAAGCC ATTGGAAAAT GAAATAGGAA AAAATGGCAA ATGCTTTACT 
TGGTGCCCAA ACTGCATGCG ACAATTCACG TTAAGCAAGG CACTGGCCAA GAAGTCGGAG 
ACTTTCACAA AACTTGGAGA AACAAAGTTT ACAAAAGTGG CCGACGAACG TCCTCCTCAG 
CGCATAGAAT TTCTAACATC AAAGGAATTC ACGCCCTCAA AATCGTCAGA AGAAGCTTTG 
GAACAGATTA TGGAAGCTCT CAAAGATGAC AACGTCAATA TGATCGGACT GTACGGCATG 
GGAGGGGTGG GTAAAACCAC CTTGGTGAAA GAAGTAGGCA GGAGAGCAAA AGAGTTGCAG 
CTTTTTCCTG AAGTTTTGAT GGCTACGGTG TCCCAGAATC AAAATGTTAC AGACATCCAG 
GATCGAATGG CAGATAAGTT ATGTCTGGAT ATTAAAGAAA AGAGTAAAGA AGGGAGAGCA 
GATCGATTAT GGCAGAGACT GAAGCAAGTG GAGAAGATGC TTATAATCCT GGATGATGTT 
TGGAAACATA TTGACTTGAA AGAGATAGGG ATCCCATTTG GTGATGATCA CAGGGGTTGT 
AAAATTCTTC TAACAACACG TCGTCGAGAC ATATGTTCTT ATATGGTGTG CCAGAAAAAT 
GTGTTTCTAC GTCTCTTTTC TGAAAAAGAA GCATGGGATT TATTCAGAAT CAATGCAGGT 
TTAGATGATG GGGACTCTAC CTTGAACAGA GTGGCAAGGG ATGTTGCGAG AGAATGCCAC 
GGCTTGCCTA TAGCACTTGT GACAATGGGA AGGGCTCTAA GAGATGAATC TGCAGTTAAG 
TGGAAAAGAG TGTCTAAACA GCTCAAAAAC TCTCAATTTC CGGACATGGA ACAAATTGAA 
GAAAAAAATG CATATGCATG TCTTAAGTTG AGCTACGATT ATTTGAAGTC CAAGGAAACC 
AAGTTATGTT TCTTGCTATG CTGTTTATTT CCAGAAGATT ACAACATTCC AGTCGAGGAC 
TTGACGAGAT ATGCAGTTGG CTATGGGTTA CATCAAGATG GGGAGCCCAT TGAAGATGCA 
AGGGAACAAG TTCATGTGGC AATCCAAGAC CTCAAATCTT GCTGTTTGTT GTTGGGCACT 
GAAACTGAAG AACATGTGAG AATGCATGAC TTGGTTCGTG ATGTTGCTAT TCAGATAGCA 
TCATCAAAAG AATATGGATT CATGGTATTG GAGAAGTGGC CATTGAGTAT TGAAAGCTTT 
GAAGCTTGTA CAACAATTTC GTTAATGGGC AATAAACTAA CCGAACTTCC TGAAGGATTG 
GAATGTCCAC AGCTCAAAGT TCTATTGTTA GAATTGGATT ATGGTTTGTA TGTTCCTCAG 
AGGTTCTTTG AAGGGATGAA AGAAATTGAA GTTTTGTCTC TGGAGGGAGG GTGTTTGTCA 
TTGCAATCAC TTGAACTCTC AACGAAACTT CAATCGTTGG TGTTGATCAG GTGTGAATGC 
AAGGACCTCA TTTGGTTGAG AAAGCTGCAG AGACTTAAGA TTCTTGTTTT TATACGGTGC 
TTATCCATTG AAGAATTACC TGATGAAATA GGAGAGCTCA AGGAGTTAAG GTTGTTGGAT 
GTGACAGGTT GTCAAAGGCT AAGAAGGATC CCTGTGAATT TGATTGGAAG GTTGAAGAAG 
TTAGAAGAGC TGTTGATCGG GCATCGCAGC TTTGATGGAT GGGATGTTGT TGGATGTGAC 
AGCACCGGAG GAATGAATGC AAGCCTGACA GAACTAAATT CGTTGTCTCA GTTAGCCGTA 
TTATCATTGA GGATCCCGAA GGTTGAATGC ACTCCCAGAG ATTTTGTTTT TCCAGTTAGC 
TTGCGCAAAT ATGATATAAT ATTAGGGAAT CCGTTTGTGG CAGCCAATTA TGGATACCCA 
ACCTCGACAA GATTAATTTT GGCTGGTACA TCCTTAAATG CGAAGACATT TGAGCAATTG 
TTTTTACATA AATTAGAATT TGTAGAAGTG ACGCATTGTG GGGATGTTTT CACTCTGTTT 
CCAGCAAGAT TGCGGCAAGG TTTAAAAAAT CTGAAGGAGG TGATTGTTGA CAGCTGTAAA 
TCATTGGAAG AGGTGTTTGA ATTGGGTGAG GCTGATGAAG GAAGCAGTGA GGAGAAGGAG 
CTGCCGCTGC TGTCATCTTT AACAGAGTTA AAGCTGCGAT GGTTACCTGA GCTCAAATGC 
ATATGGCCCA CCGGACATAT CAGCCTCCAA AATCTTGCTC ATCTGGAGTT GTTTTCTCTC 
CACAAACTGA CATTTATCTT CACACCGCCC CTCGCTCGAA GTTTGTCAAA GCTAGAAGGC 
CTTTACATAA GAAATTGCGG TGAATTGAAG CATATTATCA GAGAAGAGGA AGGTGAAAGG 
GAAATAATTC CAGAGTCTCC TTGCTTCCCA GAATTAAAAA ATATCAACAT ATATTTCTGT 
GATAAACTGG AATATGTCTT GCCAGTCTCT TTATCTCATG ACAGAGATGG CATCATCAAG 
TTCCCTCAAC TAAGAGAATT GTCTCTTAAG CTCGGATCAA ATTACAGCTT TTTAGGTCCA 
AGGAATTTTG ATGCTCAATT GCCTTTGCAA GAATTAACCA TTAAAGGCCA CGAAGAAGTG 
GGTAATTGGT TGGCACAGCT ACAACAAAAC GGCTTCTTAC AAAGATTAGA ATATGTACAC 
GTGGACGATT GTGCGGATGT TCGCACTCCG TTTCCAGCAA AATTGCTGCG AGCTTTGAAA 
AATCTAAGGA TGGTGAACAT ATACGGCTGC AAATCATTGG AAGAGGTATT TGAATTAGGT 
GAGGCTGATG AAGGAAGCAG TGAGGAGAAG GAGCTGCCGC TGCTGTCATC TTTAACAGAG 
TTACAGCTGC AAAGGTTACC TGAGCTCAAA TGCATATGGA AGGGGCCCAC CAGAAATGTC 
AGCCTCCAAA GTCTTGTTCA TCTGAATGTT TGGTGTCTCA ACAAACTGAC ATTTATCTTC 
ACATCATCCC TCGCGCAGAG TCTGCCAAAG CTAGAAAGAC TTTCCATGAG TGATTGCGGT 
GAATTGAAGC ATATTATCAG AGAAGAGGAT GGTGAAAGGG AAATAATTCC AGAGTCTCCT 
GGCTTCCCAA AATTAAAAAC TATCAGCATA TCTGACTGTG GTAAACTGGA ATATGTCCTC 
CCTGTCTCTG TGTCTCCAAG CCTTCTGAAC CTGAACGAGA TGCGGATTTT TAAAGCTCAT 
AATTTAAAGC AAATATTTTA CAGTGGAGAA GGAGATGCAC TCACCAGAGA TGGCATCATC 
AAGTTCCCTA AGCTAAGAGA ATTGTATCTT TCAAATTGCA GCTTCTTTGG TCCAAAGAAT 
TTTACTGCCC AATTGCCTTC CTTGCAAATT CTAGAAATTG ATGGCCACAA AGAATTGGGA 
AATTTGTTTG CACAGCTCCA AGGGTTGACA AATTTGGAAA CATTACGCTT GCGCTCCCTG 
CTGGTGCCTG ATATTAGGTG TCTATGGAAG GGTCTCGTGT TGAGCAAATT GACTACTTTG 
AATGTGGTTG AGTGTAAAAG ACTGACGCAT GTATTCACAC GCAGTATGAT TGTCAGTCTA 
GTTCCACTGA AAGTTCTAAA GATATTGTCC TGTGAGGAAT TGGAGCAAAT CATTGCTAAG 
GATGATGATG AAAACGACCA GATATTGTTA GGAGATCATC TCCAATCTTT ATGCTTTCCC 
AATTTGTGTG AAATTGAGAT TAGAGAATGC AACAAGTTGA AGAGTCTCTT CCCATTAGCC 
ATGGCTTCAG GTCTCCCAAA TCTCCAAATA CTGAGGGTAA CAAAAGCCTC TCAATTATTG 
GGAGTCTTTG GGCAGGATGA TCATGCTTCA CCTGTCAACG TTGAGAAGGA GATGGTGCTT 
CCTAATCTAA AAAAGTTGTC TCTAGAACAA TTATCAAGTA TTGTCTGCTT CAGTTTCGGA 
TGCTGTGATT TCTTATTCCC TCGTTTGGAG AAGTTGAAGG TGTATGAATG CTCAAAGTTG 
ACCATAAAAT TTGCTATTAC ACCAAATGGT GGTTCAATGA GTGCTCAATC AGAGGTATCT 
CAACCAGCTG AAGATTCAAG CACTAATAGA GAGTGGACCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7459153 ( XM_024585969.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024441737.1 Populus trichocarpa probable disease resistance protein At5g63020 (LOC7459153), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa422386
Clone ID Related Accession (Same CDS sequence)	XM_024585974.1 , XM_024585969.1 , XM_024585979.1 , XM_024585984.1 , XM_024585964.1
Accession Version	XM_024585969.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	probable disease resistance protein At5g63020 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTTG AAAGTGCTGG TGGATCCATT ATAGCTATGC TAGCAGAACT CATGGTGGAA 
CCAGTAGGAA GGCTGTTCCG TTACATGTTC TGTTTCAACA ATTTTGTTGA AGAATTCAAA 
GAACGAAAGG AGAACCTTGC TTTAGCACTA GATGGTCTGC AAAAAGAAGT CGAAGCTGCT 
GAAAGGAATG CTGAAGAAAT TAAGAAGGAT GTCAAAAAGT GGCTGGAAGA TGCAAAAAAC 
GAAATTGAAG GTGCGAAGCC ATTGGAAAAT GAAATAGGAA AAAATGGCAA ATGCTTTACT 
TGGTGCCCAA ACTGCATGCG ACAATTCACG TTAAGCAAGG CACTGGCCAA GAAGTCGGAG 
ACTTTCACAA AACTTGGAGA AACAAAGTTT ACAAAAGTGG CCGACGAACG TCCTCCTCAG 
CGCATAGAAT TTCTAACATC AAAGGAATTC ACGCCCTCAA AATCGTCAGA AGAAGCTTTG 
GAACAGATTA TGGAAGCTCT CAAAGATGAC AACGTCAATA TGATCGGACT GTACGGCATG 
GGAGGGGTGG GTAAAACCAC CTTGGTGAAA GAAGTAGGCA GGAGAGCAAA AGAGTTGCAG 
CTTTTTCCTG AAGTTTTGAT GGCTACGGTG TCCCAGAATC AAAATGTTAC AGACATCCAG 
GATCGAATGG CAGATAAGTT ATGTCTGGAT ATTAAAGAAA AGAGTAAAGA AGGGAGAGCA 
GATCGATTAT GGCAGAGACT GAAGCAAGTG GAGAAGATGC TTATAATCCT GGATGATGTT 
TGGAAACATA TTGACTTGAA AGAGATAGGG ATCCCATTTG GTGATGATCA CAGGGGTTGT 
AAAATTCTTC TAACAACACG TCGTCGAGAC ATATGTTCTT ATATGGTGTG CCAGAAAAAT 
GTGTTTCTAC GTCTCTTTTC TGAAAAAGAA GCATGGGATT TATTCAGAAT CAATGCAGGT 
TTAGATGATG GGGACTCTAC CTTGAACAGA GTGGCAAGGG ATGTTGCGAG AGAATGCCAC 
GGCTTGCCTA TAGCACTTGT GACAATGGGA AGGGCTCTAA GAGATGAATC TGCAGTTAAG 
TGGAAAAGAG TGTCTAAACA GCTCAAAAAC TCTCAATTTC CGGACATGGA ACAAATTGAA 
GAAAAAAATG CATATGCATG TCTTAAGTTG AGCTACGATT ATTTGAAGTC CAAGGAAACC 
AAGTTATGTT TCTTGCTATG CTGTTTATTT CCAGAAGATT ACAACATTCC AGTCGAGGAC 
TTGACGAGAT ATGCAGTTGG CTATGGGTTA CATCAAGATG GGGAGCCCAT TGAAGATGCA 
AGGGAACAAG TTCATGTGGC AATCCAAGAC CTCAAATCTT GCTGTTTGTT GTTGGGCACT 
GAAACTGAAG AACATGTGAG AATGCATGAC TTGGTTCGTG ATGTTGCTAT TCAGATAGCA 
TCATCAAAAG AATATGGATT CATGGTATTG GAGAAGTGGC CATTGAGTAT TGAAAGCTTT 
GAAGCTTGTA CAACAATTTC GTTAATGGGC AATAAACTAA CCGAACTTCC TGAAGGATTG 
GAATGTCCAC AGCTCAAAGT TCTATTGTTA GAATTGGATT ATGGTTTGTA TGTTCCTCAG 
AGGTTCTTTG AAGGGATGAA AGAAATTGAA GTTTTGTCTC TGGAGGGAGG GTGTTTGTCA 
TTGCAATCAC TTGAACTCTC AACGAAACTT CAATCGTTGG TGTTGATCAG GTGTGAATGC 
AAGGACCTCA TTTGGTTGAG AAAGCTGCAG AGACTTAAGA TTCTTGTTTT TATACGGTGC 
TTATCCATTG AAGAATTACC TGATGAAATA GGAGAGCTCA AGGAGTTAAG GTTGTTGGAT 
GTGACAGGTT GTCAAAGGCT AAGAAGGATC CCTGTGAATT TGATTGGAAG GTTGAAGAAG 
TTAGAAGAGC TGTTGATCGG GCATCGCAGC TTTGATGGAT GGGATGTTGT TGGATGTGAC 
AGCACCGGAG GAATGAATGC AAGCCTGACA GAACTAAATT CGTTGTCTCA GTTAGCCGTA 
TTATCATTGA GGATCCCGAA GGTTGAATGC ACTCCCAGAG ATTTTGTTTT TCCAGTTAGC 
TTGCGCAAAT ATGATATAAT ATTAGGGAAT CCGTTTGTGG CAGCCAATTA TGGATACCCA 
ACCTCGACAA GATTAATTTT GGCTGGTACA TCCTTAAATG CGAAGACATT TGAGCAATTG 
TTTTTACATA AATTAGAATT TGTAGAAGTG ACGCATTGTG GGGATGTTTT CACTCTGTTT 
CCAGCAAGAT TGCGGCAAGG TTTAAAAAAT CTGAAGGAGG TGATTGTTGA CAGCTGTAAA 
TCATTGGAAG AGGTGTTTGA ATTGGGTGAG GCTGATGAAG GAAGCAGTGA GGAGAAGGAG 
CTGCCGCTGC TGTCATCTTT AACAGAGTTA AAGCTGCGAT GGTTACCTGA GCTCAAATGC 
ATATGGCCCA CCGGACATAT CAGCCTCCAA AATCTTGCTC ATCTGGAGTT GTTTTCTCTC 
CACAAACTGA CATTTATCTT CACACCGCCC CTCGCTCGAA GTTTGTCAAA GCTAGAAGGC 
CTTTACATAA GAAATTGCGG TGAATTGAAG CATATTATCA GAGAAGAGGA AGGTGAAAGG 
GAAATAATTC CAGAGTCTCC TTGCTTCCCA GAATTAAAAA ATATCAACAT ATATTTCTGT 
GATAAACTGG AATATGTCTT GCCAGTCTCT TTATCTCATG ACAGAGATGG CATCATCAAG 
TTCCCTCAAC TAAGAGAATT GTCTCTTAAG CTCGGATCAA ATTACAGCTT TTTAGGTCCA 
AGGAATTTTG ATGCTCAATT GCCTTTGCAA GAATTAACCA TTAAAGGCCA CGAAGAAGTG 
GGTAATTGGT TGGCACAGCT ACAACAAAAC GGCTTCTTAC AAAGATTAGA ATATGTACAC 
GTGGACGATT GTGCGGATGT TCGCACTCCG TTTCCAGCAA AATTGCTGCG AGCTTTGAAA 
AATCTAAGGA TGGTGAACAT ATACGGCTGC AAATCATTGG AAGAGGTATT TGAATTAGGT 
GAGGCTGATG AAGGAAGCAG TGAGGAGAAG GAGCTGCCGC TGCTGTCATC TTTAACAGAG 
TTACAGCTGC AAAGGTTACC TGAGCTCAAA TGCATATGGA AGGGGCCCAC CAGAAATGTC 
AGCCTCCAAA GTCTTGTTCA TCTGAATGTT TGGTGTCTCA ACAAACTGAC ATTTATCTTC 
ACATCATCCC TCGCGCAGAG TCTGCCAAAG CTAGAAAGAC TTTCCATGAG TGATTGCGGT 
GAATTGAAGC ATATTATCAG AGAAGAGGAT GGTGAAAGGG AAATAATTCC AGAGTCTCCT 
GGCTTCCCAA AATTAAAAAC TATCAGCATA TCTGACTGTG GTAAACTGGA ATATGTCCTC 
CCTGTCTCTG TGTCTCCAAG CCTTCTGAAC CTGAACGAGA TGCGGATTTT TAAAGCTCAT 
AATTTAAAGC AAATATTTTA CAGTGGAGAA GGAGATGCAC TCACCAGAGA TGGCATCATC 
AAGTTCCCTA AGCTAAGAGA ATTGTATCTT TCAAATTGCA GCTTCTTTGG TCCAAAGAAT 
TTTACTGCCC AATTGCCTTC CTTGCAAATT CTAGAAATTG ATGGCCACAA AGAATTGGGA 
AATTTGTTTG CACAGCTCCA AGGGTTGACA AATTTGGAAA CATTACGCTT GCGCTCCCTG 
CTGGTGCCTG ATATTAGGTG TCTATGGAAG GGTCTCGTGT TGAGCAAATT GACTACTTTG 
AATGTGGTTG AGTGTAAAAG ACTGACGCAT GTATTCACAC GCAGTATGAT TGTCAGTCTA 
GTTCCACTGA AAGTTCTAAA GATATTGTCC TGTGAGGAAT TGGAGCAAAT CATTGCTAAG 
GATGATGATG AAAACGACCA GATATTGTTA GGAGATCATC TCCAATCTTT ATGCTTTCCC 
AATTTGTGTG AAATTGAGAT TAGAGAATGC AACAAGTTGA AGAGTCTCTT CCCATTAGCC 
ATGGCTTCAG GTCTCCCAAA TCTCCAAATA CTGAGGGTAA CAAAAGCCTC TCAATTATTG 
GGAGTCTTTG GGCAGGATGA TCATGCTTCA CCTGTCAACG TTGAGAAGGA GATGGTGCTT 
CCTAATCTAA AAAAGTTGTC TCTAGAACAA TTATCAAGTA TTGTCTGCTT CAGTTTCGGA 
TGCTGTGATT TCTTATTCCC TCGTTTGGAG AAGTTGAAGG TGTATGAATG CTCAAAGTTG 
ACCATAAAAT TTGCTATTAC ACCAAATGGT GGTTCAATGA GTGCTCAATC AGAGGTATCT 
CAACCAGCTG AAGATTCAAG CACTAATAGA GAGTGGACCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024441737.1
CDS	532..4896
Translation	MALESAGGSIIAMLAELMVEPVGRLFRYMFCFNNFVEEFKERKENLALALDGLQKEVEAAERNAEEIKKDVKKWLEDAKNEIEGAKPLENEIGKNGKCFTWCPNCMRQFTLSKALAKKSETFTKLGETKFTKVADERPPQRIEFLTSKEFTPSKSSEEALEQIMEALKDDNVNMIGLYGMGGVGKTTLVKEVGRRAKELQLFPEVLMATVSQNQNVTDIQDRMADKLCLDIKEKSKEGRADRLWQRLKQVEKMLIILDDVWKHIDLKEIGIPFGDDHRGCKILLTTRRRDICSYMVCQKNVFLRLFSEKEAWDLFRINAGLDDGDSTLNRVARDVARECHGLPIALVTMGRALRDESAVKWKRVSKQLKNSQFPDMEQIEEKNAYACLKLSYDYLKSKETKLCFLLCCLFPEDYNIPVEDLTRYAVGYGLHQDGEPIEDAREQVHVAIQDLKSCCLLLGTETEEHVRMHDLVRDVAIQIASSKEYGFMVLEKWPLSIESFEACTTISLMGNKLTELPEGLECPQLKVLLLELDYGLYVPQRFFEGMKEIEVLSLEGGCLSLQSLELSTKLQSLVLIRCECKDLIWLRKLQRLKILVFIRCLSIEELPDEIGELKELRLLDVTGCQRLRRIPVNLIGRLKKLEELLIGHRSFDGWDVVGCDSTGGMNASLTELNSLSQLAVLSLRIPKVECTPRDFVFPVSLRKYDIILGNPFVAANYGYPTSTRLILAGTSLNAKTFEQLFLHKLEFVEVTHCGDVFTLFPARLRQGLKNLKEVIVDSCKSLEEVFELGEADEGSSEEKELPLLSSLTELKLRWLPELKCIWPTGHISLQNLAHLELFSLHKLTFIFTPPLARSLSKLEGLYIRNCGELKHIIREEEGEREIIPESPCFPELKNINIYFCDKLEYVLPVSLSHDRDGIIKFPQLRELSLKLGSNYSFLGPRNFDAQLPLQELTIKGHEEVGNWLAQLQQNGFLQRLEYVHVDDCADVRTPFPAKLLRALKNLRMVNIYGCKSLEEVFELGEADEGSSEEKELPLLSSLTELQLQRLPELKCIWKGPTRNVSLQSLVHLNVWCLNKLTFIFTSSLAQSLPKLERLSMSDCGELKHIIREEDGEREIIPESPGFPKLKTISISDCGKLEYVLPVSVSPSLLNLNEMRIFKAHNLKQIFYSGEGDALTRDGIIKFPKLRELYLSNCSFFGPKNFTAQLPSLQILEIDGHKELGNLFAQLQGLTNLETLRLRSLLVPDIRCLWKGLVLSKLTTLNVVECKRLTHVFTRSMIVSLVPLKVLKILSCEELEQIIAKDDDENDQILLGDHLQSLCFPNLCEIEIRECNKLKSLFPLAMASGLPNLQILRVTKASQLLGVFGQDDHASPVNVEKEMVLPNLKKLSLEQLSSIVCFSFGCCDFLFPRLEKLKVYECSKLTIKFAITPNGGSMSAQSEVSQPAEDSSTNREWTS

Target ORF information:

RefSeq Version	XM_024585969.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa probable disease resistance protein At5g63020 (LOC7459153), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024585969.1

ORF Insert Sequence:

ATGGCTCTTG AAAGTGCTGG TGGATCCATT ATAGCTATGC TAGCAGAACT CATGGTGGAA 
CCAGTAGGAA GGCTGTTCCG TTACATGTTC TGTTTCAACA ATTTTGTTGA AGAATTCAAA 
GAACGAAAGG AGAACCTTGC TTTAGCACTA GATGGTCTGC AAAAAGAAGT CGAAGCTGCT 
GAAAGGAATG CTGAAGAAAT TAAGAAGGAT GTCAAAAAGT GGCTGGAAGA TGCAAAAAAC 
GAAATTGAAG GTGCGAAGCC ATTGGAAAAT GAAATAGGAA AAAATGGCAA ATGCTTTACT 
TGGTGCCCAA ACTGCATGCG ACAATTCACG TTAAGCAAGG CACTGGCCAA GAAGTCGGAG 
ACTTTCACAA AACTTGGAGA AACAAAGTTT ACAAAAGTGG CCGACGAACG TCCTCCTCAG 
CGCATAGAAT TTCTAACATC AAAGGAATTC ACGCCCTCAA AATCGTCAGA AGAAGCTTTG 
GAACAGATTA TGGAAGCTCT CAAAGATGAC AACGTCAATA TGATCGGACT GTACGGCATG 
GGAGGGGTGG GTAAAACCAC CTTGGTGAAA GAAGTAGGCA GGAGAGCAAA AGAGTTGCAG 
CTTTTTCCTG AAGTTTTGAT GGCTACGGTG TCCCAGAATC AAAATGTTAC AGACATCCAG 
GATCGAATGG CAGATAAGTT ATGTCTGGAT ATTAAAGAAA AGAGTAAAGA AGGGAGAGCA 
GATCGATTAT GGCAGAGACT GAAGCAAGTG GAGAAGATGC TTATAATCCT GGATGATGTT 
TGGAAACATA TTGACTTGAA AGAGATAGGG ATCCCATTTG GTGATGATCA CAGGGGTTGT 
AAAATTCTTC TAACAACACG TCGTCGAGAC ATATGTTCTT ATATGGTGTG CCAGAAAAAT 
GTGTTTCTAC GTCTCTTTTC TGAAAAAGAA GCATGGGATT TATTCAGAAT CAATGCAGGT 
TTAGATGATG GGGACTCTAC CTTGAACAGA GTGGCAAGGG ATGTTGCGAG AGAATGCCAC 
GGCTTGCCTA TAGCACTTGT GACAATGGGA AGGGCTCTAA GAGATGAATC TGCAGTTAAG 
TGGAAAAGAG TGTCTAAACA GCTCAAAAAC TCTCAATTTC CGGACATGGA ACAAATTGAA 
GAAAAAAATG CATATGCATG TCTTAAGTTG AGCTACGATT ATTTGAAGTC CAAGGAAACC 
AAGTTATGTT TCTTGCTATG CTGTTTATTT CCAGAAGATT ACAACATTCC AGTCGAGGAC 
TTGACGAGAT ATGCAGTTGG CTATGGGTTA CATCAAGATG GGGAGCCCAT TGAAGATGCA 
AGGGAACAAG TTCATGTGGC AATCCAAGAC CTCAAATCTT GCTGTTTGTT GTTGGGCACT 
GAAACTGAAG AACATGTGAG AATGCATGAC TTGGTTCGTG ATGTTGCTAT TCAGATAGCA 
TCATCAAAAG AATATGGATT CATGGTATTG GAGAAGTGGC CATTGAGTAT TGAAAGCTTT 
GAAGCTTGTA CAACAATTTC GTTAATGGGC AATAAACTAA CCGAACTTCC TGAAGGATTG 
GAATGTCCAC AGCTCAAAGT TCTATTGTTA GAATTGGATT ATGGTTTGTA TGTTCCTCAG 
AGGTTCTTTG AAGGGATGAA AGAAATTGAA GTTTTGTCTC TGGAGGGAGG GTGTTTGTCA 
TTGCAATCAC TTGAACTCTC AACGAAACTT CAATCGTTGG TGTTGATCAG GTGTGAATGC 
AAGGACCTCA TTTGGTTGAG AAAGCTGCAG AGACTTAAGA TTCTTGTTTT TATACGGTGC 
TTATCCATTG AAGAATTACC TGATGAAATA GGAGAGCTCA AGGAGTTAAG GTTGTTGGAT 
GTGACAGGTT GTCAAAGGCT AAGAAGGATC CCTGTGAATT TGATTGGAAG GTTGAAGAAG 
TTAGAAGAGC TGTTGATCGG GCATCGCAGC TTTGATGGAT GGGATGTTGT TGGATGTGAC 
AGCACCGGAG GAATGAATGC AAGCCTGACA GAACTAAATT CGTTGTCTCA GTTAGCCGTA 
TTATCATTGA GGATCCCGAA GGTTGAATGC ACTCCCAGAG ATTTTGTTTT TCCAGTTAGC 
TTGCGCAAAT ATGATATAAT ATTAGGGAAT CCGTTTGTGG CAGCCAATTA TGGATACCCA 
ACCTCGACAA GATTAATTTT GGCTGGTACA TCCTTAAATG CGAAGACATT TGAGCAATTG 
TTTTTACATA AATTAGAATT TGTAGAAGTG ACGCATTGTG GGGATGTTTT CACTCTGTTT 
CCAGCAAGAT TGCGGCAAGG TTTAAAAAAT CTGAAGGAGG TGATTGTTGA CAGCTGTAAA 
TCATTGGAAG AGGTGTTTGA ATTGGGTGAG GCTGATGAAG GAAGCAGTGA GGAGAAGGAG 
CTGCCGCTGC TGTCATCTTT AACAGAGTTA AAGCTGCGAT GGTTACCTGA GCTCAAATGC 
ATATGGCCCA CCGGACATAT CAGCCTCCAA AATCTTGCTC ATCTGGAGTT GTTTTCTCTC 
CACAAACTGA CATTTATCTT CACACCGCCC CTCGCTCGAA GTTTGTCAAA GCTAGAAGGC 
CTTTACATAA GAAATTGCGG TGAATTGAAG CATATTATCA GAGAAGAGGA AGGTGAAAGG 
GAAATAATTC CAGAGTCTCC TTGCTTCCCA GAATTAAAAA ATATCAACAT ATATTTCTGT 
GATAAACTGG AATATGTCTT GCCAGTCTCT TTATCTCATG ACAGAGATGG CATCATCAAG 
TTCCCTCAAC TAAGAGAATT GTCTCTTAAG CTCGGATCAA ATTACAGCTT TTTAGGTCCA 
AGGAATTTTG ATGCTCAATT GCCTTTGCAA GAATTAACCA TTAAAGGCCA CGAAGAAGTG 
GGTAATTGGT TGGCACAGCT ACAACAAAAC GGCTTCTTAC AAAGATTAGA ATATGTACAC 
GTGGACGATT GTGCGGATGT TCGCACTCCG TTTCCAGCAA AATTGCTGCG AGCTTTGAAA 
AATCTAAGGA TGGTGAACAT ATACGGCTGC AAATCATTGG AAGAGGTATT TGAATTAGGT 
GAGGCTGATG AAGGAAGCAG TGAGGAGAAG GAGCTGCCGC TGCTGTCATC TTTAACAGAG 
TTACAGCTGC AAAGGTTACC TGAGCTCAAA TGCATATGGA AGGGGCCCAC CAGAAATGTC 
AGCCTCCAAA GTCTTGTTCA TCTGAATGTT TGGTGTCTCA ACAAACTGAC ATTTATCTTC 
ACATCATCCC TCGCGCAGAG TCTGCCAAAG CTAGAAAGAC TTTCCATGAG TGATTGCGGT 
GAATTGAAGC ATATTATCAG AGAAGAGGAT GGTGAAAGGG AAATAATTCC AGAGTCTCCT 
GGCTTCCCAA AATTAAAAAC TATCAGCATA TCTGACTGTG GTAAACTGGA ATATGTCCTC 
CCTGTCTCTG TGTCTCCAAG CCTTCTGAAC CTGAACGAGA TGCGGATTTT TAAAGCTCAT 
AATTTAAAGC AAATATTTTA CAGTGGAGAA GGAGATGCAC TCACCAGAGA TGGCATCATC 
AAGTTCCCTA AGCTAAGAGA ATTGTATCTT TCAAATTGCA GCTTCTTTGG TCCAAAGAAT 
TTTACTGCCC AATTGCCTTC CTTGCAAATT CTAGAAATTG ATGGCCACAA AGAATTGGGA 
AATTTGTTTG CACAGCTCCA AGGGTTGACA AATTTGGAAA CATTACGCTT GCGCTCCCTG 
CTGGTGCCTG ATATTAGGTG TCTATGGAAG GGTCTCGTGT TGAGCAAATT GACTACTTTG 
AATGTGGTTG AGTGTAAAAG ACTGACGCAT GTATTCACAC GCAGTATGAT TGTCAGTCTA 
GTTCCACTGA AAGTTCTAAA GATATTGTCC TGTGAGGAAT TGGAGCAAAT CATTGCTAAG 
GATGATGATG AAAACGACCA GATATTGTTA GGAGATCATC TCCAATCTTT ATGCTTTCCC 
AATTTGTGTG AAATTGAGAT TAGAGAATGC AACAAGTTGA AGAGTCTCTT CCCATTAGCC 
ATGGCTTCAG GTCTCCCAAA TCTCCAAATA CTGAGGGTAA CAAAAGCCTC TCAATTATTG 
GGAGTCTTTG GGCAGGATGA TCATGCTTCA CCTGTCAACG TTGAGAAGGA GATGGTGCTT 
CCTAATCTAA AAAAGTTGTC TCTAGAACAA TTATCAAGTA TTGTCTGCTT CAGTTTCGGA 
TGCTGTGATT TCTTATTCCC TCGTTTGGAG AAGTTGAAGG TGTATGAATG CTCAAAGTTG 
ACCATAAAAT TTGCTATTAC ACCAAATGGT GGTTCAATGA GTGCTCAATC AGAGGTATCT 
CAACCAGCTG AAGATTCAAG CACTAATAGA GAGTGGACCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7459153 ( XM_024585979.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024441747.1 Populus trichocarpa probable disease resistance protein At5g63020 (LOC7459153), transcript variant X5, mRNA.

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CloneID	OPa422386
Clone ID Related Accession (Same CDS sequence)	XM_024585974.1 , XM_024585969.1 , XM_024585979.1 , XM_024585984.1 , XM_024585964.1
Accession Version	XM_024585979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	probable disease resistance protein At5g63020 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTTG AAAGTGCTGG TGGATCCATT ATAGCTATGC TAGCAGAACT CATGGTGGAA 
CCAGTAGGAA GGCTGTTCCG TTACATGTTC TGTTTCAACA ATTTTGTTGA AGAATTCAAA 
GAACGAAAGG AGAACCTTGC TTTAGCACTA GATGGTCTGC AAAAAGAAGT CGAAGCTGCT 
GAAAGGAATG CTGAAGAAAT TAAGAAGGAT GTCAAAAAGT GGCTGGAAGA TGCAAAAAAC 
GAAATTGAAG GTGCGAAGCC ATTGGAAAAT GAAATAGGAA AAAATGGCAA ATGCTTTACT 
TGGTGCCCAA ACTGCATGCG ACAATTCACG TTAAGCAAGG CACTGGCCAA GAAGTCGGAG 
ACTTTCACAA AACTTGGAGA AACAAAGTTT ACAAAAGTGG CCGACGAACG TCCTCCTCAG 
CGCATAGAAT TTCTAACATC AAAGGAATTC ACGCCCTCAA AATCGTCAGA AGAAGCTTTG 
GAACAGATTA TGGAAGCTCT CAAAGATGAC AACGTCAATA TGATCGGACT GTACGGCATG 
GGAGGGGTGG GTAAAACCAC CTTGGTGAAA GAAGTAGGCA GGAGAGCAAA AGAGTTGCAG 
CTTTTTCCTG AAGTTTTGAT GGCTACGGTG TCCCAGAATC AAAATGTTAC AGACATCCAG 
GATCGAATGG CAGATAAGTT ATGTCTGGAT ATTAAAGAAA AGAGTAAAGA AGGGAGAGCA 
GATCGATTAT GGCAGAGACT GAAGCAAGTG GAGAAGATGC TTATAATCCT GGATGATGTT 
TGGAAACATA TTGACTTGAA AGAGATAGGG ATCCCATTTG GTGATGATCA CAGGGGTTGT 
AAAATTCTTC TAACAACACG TCGTCGAGAC ATATGTTCTT ATATGGTGTG CCAGAAAAAT 
GTGTTTCTAC GTCTCTTTTC TGAAAAAGAA GCATGGGATT TATTCAGAAT CAATGCAGGT 
TTAGATGATG GGGACTCTAC CTTGAACAGA GTGGCAAGGG ATGTTGCGAG AGAATGCCAC 
GGCTTGCCTA TAGCACTTGT GACAATGGGA AGGGCTCTAA GAGATGAATC TGCAGTTAAG 
TGGAAAAGAG TGTCTAAACA GCTCAAAAAC TCTCAATTTC CGGACATGGA ACAAATTGAA 
GAAAAAAATG CATATGCATG TCTTAAGTTG AGCTACGATT ATTTGAAGTC CAAGGAAACC 
AAGTTATGTT TCTTGCTATG CTGTTTATTT CCAGAAGATT ACAACATTCC AGTCGAGGAC 
TTGACGAGAT ATGCAGTTGG CTATGGGTTA CATCAAGATG GGGAGCCCAT TGAAGATGCA 
AGGGAACAAG TTCATGTGGC AATCCAAGAC CTCAAATCTT GCTGTTTGTT GTTGGGCACT 
GAAACTGAAG AACATGTGAG AATGCATGAC TTGGTTCGTG ATGTTGCTAT TCAGATAGCA 
TCATCAAAAG AATATGGATT CATGGTATTG GAGAAGTGGC CATTGAGTAT TGAAAGCTTT 
GAAGCTTGTA CAACAATTTC GTTAATGGGC AATAAACTAA CCGAACTTCC TGAAGGATTG 
GAATGTCCAC AGCTCAAAGT TCTATTGTTA GAATTGGATT ATGGTTTGTA TGTTCCTCAG 
AGGTTCTTTG AAGGGATGAA AGAAATTGAA GTTTTGTCTC TGGAGGGAGG GTGTTTGTCA 
TTGCAATCAC TTGAACTCTC AACGAAACTT CAATCGTTGG TGTTGATCAG GTGTGAATGC 
AAGGACCTCA TTTGGTTGAG AAAGCTGCAG AGACTTAAGA TTCTTGTTTT TATACGGTGC 
TTATCCATTG AAGAATTACC TGATGAAATA GGAGAGCTCA AGGAGTTAAG GTTGTTGGAT 
GTGACAGGTT GTCAAAGGCT AAGAAGGATC CCTGTGAATT TGATTGGAAG GTTGAAGAAG 
TTAGAAGAGC TGTTGATCGG GCATCGCAGC TTTGATGGAT GGGATGTTGT TGGATGTGAC 
AGCACCGGAG GAATGAATGC AAGCCTGACA GAACTAAATT CGTTGTCTCA GTTAGCCGTA 
TTATCATTGA GGATCCCGAA GGTTGAATGC ACTCCCAGAG ATTTTGTTTT TCCAGTTAGC 
TTGCGCAAAT ATGATATAAT ATTAGGGAAT CCGTTTGTGG CAGCCAATTA TGGATACCCA 
ACCTCGACAA GATTAATTTT GGCTGGTACA TCCTTAAATG CGAAGACATT TGAGCAATTG 
TTTTTACATA AATTAGAATT TGTAGAAGTG ACGCATTGTG GGGATGTTTT CACTCTGTTT 
CCAGCAAGAT TGCGGCAAGG TTTAAAAAAT CTGAAGGAGG TGATTGTTGA CAGCTGTAAA 
TCATTGGAAG AGGTGTTTGA ATTGGGTGAG GCTGATGAAG GAAGCAGTGA GGAGAAGGAG 
CTGCCGCTGC TGTCATCTTT AACAGAGTTA AAGCTGCGAT GGTTACCTGA GCTCAAATGC 
ATATGGCCCA CCGGACATAT CAGCCTCCAA AATCTTGCTC ATCTGGAGTT GTTTTCTCTC 
CACAAACTGA CATTTATCTT CACACCGCCC CTCGCTCGAA GTTTGTCAAA GCTAGAAGGC 
CTTTACATAA GAAATTGCGG TGAATTGAAG CATATTATCA GAGAAGAGGA AGGTGAAAGG 
GAAATAATTC CAGAGTCTCC TTGCTTCCCA GAATTAAAAA ATATCAACAT ATATTTCTGT 
GATAAACTGG AATATGTCTT GCCAGTCTCT TTATCTCATG ACAGAGATGG CATCATCAAG 
TTCCCTCAAC TAAGAGAATT GTCTCTTAAG CTCGGATCAA ATTACAGCTT TTTAGGTCCA 
AGGAATTTTG ATGCTCAATT GCCTTTGCAA GAATTAACCA TTAAAGGCCA CGAAGAAGTG 
GGTAATTGGT TGGCACAGCT ACAACAAAAC GGCTTCTTAC AAAGATTAGA ATATGTACAC 
GTGGACGATT GTGCGGATGT TCGCACTCCG TTTCCAGCAA AATTGCTGCG AGCTTTGAAA 
AATCTAAGGA TGGTGAACAT ATACGGCTGC AAATCATTGG AAGAGGTATT TGAATTAGGT 
GAGGCTGATG AAGGAAGCAG TGAGGAGAAG GAGCTGCCGC TGCTGTCATC TTTAACAGAG 
TTACAGCTGC AAAGGTTACC TGAGCTCAAA TGCATATGGA AGGGGCCCAC CAGAAATGTC 
AGCCTCCAAA GTCTTGTTCA TCTGAATGTT TGGTGTCTCA ACAAACTGAC ATTTATCTTC 
ACATCATCCC TCGCGCAGAG TCTGCCAAAG CTAGAAAGAC TTTCCATGAG TGATTGCGGT 
GAATTGAAGC ATATTATCAG AGAAGAGGAT GGTGAAAGGG AAATAATTCC AGAGTCTCCT 
GGCTTCCCAA AATTAAAAAC TATCAGCATA TCTGACTGTG GTAAACTGGA ATATGTCCTC 
CCTGTCTCTG TGTCTCCAAG CCTTCTGAAC CTGAACGAGA TGCGGATTTT TAAAGCTCAT 
AATTTAAAGC AAATATTTTA CAGTGGAGAA GGAGATGCAC TCACCAGAGA TGGCATCATC 
AAGTTCCCTA AGCTAAGAGA ATTGTATCTT TCAAATTGCA GCTTCTTTGG TCCAAAGAAT 
TTTACTGCCC AATTGCCTTC CTTGCAAATT CTAGAAATTG ATGGCCACAA AGAATTGGGA 
AATTTGTTTG CACAGCTCCA AGGGTTGACA AATTTGGAAA CATTACGCTT GCGCTCCCTG 
CTGGTGCCTG ATATTAGGTG TCTATGGAAG GGTCTCGTGT TGAGCAAATT GACTACTTTG 
AATGTGGTTG AGTGTAAAAG ACTGACGCAT GTATTCACAC GCAGTATGAT TGTCAGTCTA 
GTTCCACTGA AAGTTCTAAA GATATTGTCC TGTGAGGAAT TGGAGCAAAT CATTGCTAAG 
GATGATGATG AAAACGACCA GATATTGTTA GGAGATCATC TCCAATCTTT ATGCTTTCCC 
AATTTGTGTG AAATTGAGAT TAGAGAATGC AACAAGTTGA AGAGTCTCTT CCCATTAGCC 
ATGGCTTCAG GTCTCCCAAA TCTCCAAATA CTGAGGGTAA CAAAAGCCTC TCAATTATTG 
GGAGTCTTTG GGCAGGATGA TCATGCTTCA CCTGTCAACG TTGAGAAGGA GATGGTGCTT 
CCTAATCTAA AAAAGTTGTC TCTAGAACAA TTATCAAGTA TTGTCTGCTT CAGTTTCGGA 
TGCTGTGATT TCTTATTCCC TCGTTTGGAG AAGTTGAAGG TGTATGAATG CTCAAAGTTG 
ACCATAAAAT TTGCTATTAC ACCAAATGGT GGTTCAATGA GTGCTCAATC AGAGGTATCT 
CAACCAGCTG AAGATTCAAG CACTAATAGA GAGTGGACCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024441747.1
CDS	443..4807
Translation	MALESAGGSIIAMLAELMVEPVGRLFRYMFCFNNFVEEFKERKENLALALDGLQKEVEAAERNAEEIKKDVKKWLEDAKNEIEGAKPLENEIGKNGKCFTWCPNCMRQFTLSKALAKKSETFTKLGETKFTKVADERPPQRIEFLTSKEFTPSKSSEEALEQIMEALKDDNVNMIGLYGMGGVGKTTLVKEVGRRAKELQLFPEVLMATVSQNQNVTDIQDRMADKLCLDIKEKSKEGRADRLWQRLKQVEKMLIILDDVWKHIDLKEIGIPFGDDHRGCKILLTTRRRDICSYMVCQKNVFLRLFSEKEAWDLFRINAGLDDGDSTLNRVARDVARECHGLPIALVTMGRALRDESAVKWKRVSKQLKNSQFPDMEQIEEKNAYACLKLSYDYLKSKETKLCFLLCCLFPEDYNIPVEDLTRYAVGYGLHQDGEPIEDAREQVHVAIQDLKSCCLLLGTETEEHVRMHDLVRDVAIQIASSKEYGFMVLEKWPLSIESFEACTTISLMGNKLTELPEGLECPQLKVLLLELDYGLYVPQRFFEGMKEIEVLSLEGGCLSLQSLELSTKLQSLVLIRCECKDLIWLRKLQRLKILVFIRCLSIEELPDEIGELKELRLLDVTGCQRLRRIPVNLIGRLKKLEELLIGHRSFDGWDVVGCDSTGGMNASLTELNSLSQLAVLSLRIPKVECTPRDFVFPVSLRKYDIILGNPFVAANYGYPTSTRLILAGTSLNAKTFEQLFLHKLEFVEVTHCGDVFTLFPARLRQGLKNLKEVIVDSCKSLEEVFELGEADEGSSEEKELPLLSSLTELKLRWLPELKCIWPTGHISLQNLAHLELFSLHKLTFIFTPPLARSLSKLEGLYIRNCGELKHIIREEEGEREIIPESPCFPELKNINIYFCDKLEYVLPVSLSHDRDGIIKFPQLRELSLKLGSNYSFLGPRNFDAQLPLQELTIKGHEEVGNWLAQLQQNGFLQRLEYVHVDDCADVRTPFPAKLLRALKNLRMVNIYGCKSLEEVFELGEADEGSSEEKELPLLSSLTELQLQRLPELKCIWKGPTRNVSLQSLVHLNVWCLNKLTFIFTSSLAQSLPKLERLSMSDCGELKHIIREEDGEREIIPESPGFPKLKTISISDCGKLEYVLPVSVSPSLLNLNEMRIFKAHNLKQIFYSGEGDALTRDGIIKFPKLRELYLSNCSFFGPKNFTAQLPSLQILEIDGHKELGNLFAQLQGLTNLETLRLRSLLVPDIRCLWKGLVLSKLTTLNVVECKRLTHVFTRSMIVSLVPLKVLKILSCEELEQIIAKDDDENDQILLGDHLQSLCFPNLCEIEIRECNKLKSLFPLAMASGLPNLQILRVTKASQLLGVFGQDDHASPVNVEKEMVLPNLKKLSLEQLSSIVCFSFGCCDFLFPRLEKLKVYECSKLTIKFAITPNGGSMSAQSEVSQPAEDSSTNREWTS

Target ORF information:

RefSeq Version	XM_024585979.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa probable disease resistance protein At5g63020 (LOC7459153), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024585979.1

ORF Insert Sequence:

ATGGCTCTTG AAAGTGCTGG TGGATCCATT ATAGCTATGC TAGCAGAACT CATGGTGGAA 
CCAGTAGGAA GGCTGTTCCG TTACATGTTC TGTTTCAACA ATTTTGTTGA AGAATTCAAA 
GAACGAAAGG AGAACCTTGC TTTAGCACTA GATGGTCTGC AAAAAGAAGT CGAAGCTGCT 
GAAAGGAATG CTGAAGAAAT TAAGAAGGAT GTCAAAAAGT GGCTGGAAGA TGCAAAAAAC 
GAAATTGAAG GTGCGAAGCC ATTGGAAAAT GAAATAGGAA AAAATGGCAA ATGCTTTACT 
TGGTGCCCAA ACTGCATGCG ACAATTCACG TTAAGCAAGG CACTGGCCAA GAAGTCGGAG 
ACTTTCACAA AACTTGGAGA AACAAAGTTT ACAAAAGTGG CCGACGAACG TCCTCCTCAG 
CGCATAGAAT TTCTAACATC AAAGGAATTC ACGCCCTCAA AATCGTCAGA AGAAGCTTTG 
GAACAGATTA TGGAAGCTCT CAAAGATGAC AACGTCAATA TGATCGGACT GTACGGCATG 
GGAGGGGTGG GTAAAACCAC CTTGGTGAAA GAAGTAGGCA GGAGAGCAAA AGAGTTGCAG 
CTTTTTCCTG AAGTTTTGAT GGCTACGGTG TCCCAGAATC AAAATGTTAC AGACATCCAG 
GATCGAATGG CAGATAAGTT ATGTCTGGAT ATTAAAGAAA AGAGTAAAGA AGGGAGAGCA 
GATCGATTAT GGCAGAGACT GAAGCAAGTG GAGAAGATGC TTATAATCCT GGATGATGTT 
TGGAAACATA TTGACTTGAA AGAGATAGGG ATCCCATTTG GTGATGATCA CAGGGGTTGT 
AAAATTCTTC TAACAACACG TCGTCGAGAC ATATGTTCTT ATATGGTGTG CCAGAAAAAT 
GTGTTTCTAC GTCTCTTTTC TGAAAAAGAA GCATGGGATT TATTCAGAAT CAATGCAGGT 
TTAGATGATG GGGACTCTAC CTTGAACAGA GTGGCAAGGG ATGTTGCGAG AGAATGCCAC 
GGCTTGCCTA TAGCACTTGT GACAATGGGA AGGGCTCTAA GAGATGAATC TGCAGTTAAG 
TGGAAAAGAG TGTCTAAACA GCTCAAAAAC TCTCAATTTC CGGACATGGA ACAAATTGAA 
GAAAAAAATG CATATGCATG TCTTAAGTTG AGCTACGATT ATTTGAAGTC CAAGGAAACC 
AAGTTATGTT TCTTGCTATG CTGTTTATTT CCAGAAGATT ACAACATTCC AGTCGAGGAC 
TTGACGAGAT ATGCAGTTGG CTATGGGTTA CATCAAGATG GGGAGCCCAT TGAAGATGCA 
AGGGAACAAG TTCATGTGGC AATCCAAGAC CTCAAATCTT GCTGTTTGTT GTTGGGCACT 
GAAACTGAAG AACATGTGAG AATGCATGAC TTGGTTCGTG ATGTTGCTAT TCAGATAGCA 
TCATCAAAAG AATATGGATT CATGGTATTG GAGAAGTGGC CATTGAGTAT TGAAAGCTTT 
GAAGCTTGTA CAACAATTTC GTTAATGGGC AATAAACTAA CCGAACTTCC TGAAGGATTG 
GAATGTCCAC AGCTCAAAGT TCTATTGTTA GAATTGGATT ATGGTTTGTA TGTTCCTCAG 
AGGTTCTTTG AAGGGATGAA AGAAATTGAA GTTTTGTCTC TGGAGGGAGG GTGTTTGTCA 
TTGCAATCAC TTGAACTCTC AACGAAACTT CAATCGTTGG TGTTGATCAG GTGTGAATGC 
AAGGACCTCA TTTGGTTGAG AAAGCTGCAG AGACTTAAGA TTCTTGTTTT TATACGGTGC 
TTATCCATTG AAGAATTACC TGATGAAATA GGAGAGCTCA AGGAGTTAAG GTTGTTGGAT 
GTGACAGGTT GTCAAAGGCT AAGAAGGATC CCTGTGAATT TGATTGGAAG GTTGAAGAAG 
TTAGAAGAGC TGTTGATCGG GCATCGCAGC TTTGATGGAT GGGATGTTGT TGGATGTGAC 
AGCACCGGAG GAATGAATGC AAGCCTGACA GAACTAAATT CGTTGTCTCA GTTAGCCGTA 
TTATCATTGA GGATCCCGAA GGTTGAATGC ACTCCCAGAG ATTTTGTTTT TCCAGTTAGC 
TTGCGCAAAT ATGATATAAT ATTAGGGAAT CCGTTTGTGG CAGCCAATTA TGGATACCCA 
ACCTCGACAA GATTAATTTT GGCTGGTACA TCCTTAAATG CGAAGACATT TGAGCAATTG 
TTTTTACATA AATTAGAATT TGTAGAAGTG ACGCATTGTG GGGATGTTTT CACTCTGTTT 
CCAGCAAGAT TGCGGCAAGG TTTAAAAAAT CTGAAGGAGG TGATTGTTGA CAGCTGTAAA 
TCATTGGAAG AGGTGTTTGA ATTGGGTGAG GCTGATGAAG GAAGCAGTGA GGAGAAGGAG 
CTGCCGCTGC TGTCATCTTT AACAGAGTTA AAGCTGCGAT GGTTACCTGA GCTCAAATGC 
ATATGGCCCA CCGGACATAT CAGCCTCCAA AATCTTGCTC ATCTGGAGTT GTTTTCTCTC 
CACAAACTGA CATTTATCTT CACACCGCCC CTCGCTCGAA GTTTGTCAAA GCTAGAAGGC 
CTTTACATAA GAAATTGCGG TGAATTGAAG CATATTATCA GAGAAGAGGA AGGTGAAAGG 
GAAATAATTC CAGAGTCTCC TTGCTTCCCA GAATTAAAAA ATATCAACAT ATATTTCTGT 
GATAAACTGG AATATGTCTT GCCAGTCTCT TTATCTCATG ACAGAGATGG CATCATCAAG 
TTCCCTCAAC TAAGAGAATT GTCTCTTAAG CTCGGATCAA ATTACAGCTT TTTAGGTCCA 
AGGAATTTTG ATGCTCAATT GCCTTTGCAA GAATTAACCA TTAAAGGCCA CGAAGAAGTG 
GGTAATTGGT TGGCACAGCT ACAACAAAAC GGCTTCTTAC AAAGATTAGA ATATGTACAC 
GTGGACGATT GTGCGGATGT TCGCACTCCG TTTCCAGCAA AATTGCTGCG AGCTTTGAAA 
AATCTAAGGA TGGTGAACAT ATACGGCTGC AAATCATTGG AAGAGGTATT TGAATTAGGT 
GAGGCTGATG AAGGAAGCAG TGAGGAGAAG GAGCTGCCGC TGCTGTCATC TTTAACAGAG 
TTACAGCTGC AAAGGTTACC TGAGCTCAAA TGCATATGGA AGGGGCCCAC CAGAAATGTC 
AGCCTCCAAA GTCTTGTTCA TCTGAATGTT TGGTGTCTCA ACAAACTGAC ATTTATCTTC 
ACATCATCCC TCGCGCAGAG TCTGCCAAAG CTAGAAAGAC TTTCCATGAG TGATTGCGGT 
GAATTGAAGC ATATTATCAG AGAAGAGGAT GGTGAAAGGG AAATAATTCC AGAGTCTCCT 
GGCTTCCCAA AATTAAAAAC TATCAGCATA TCTGACTGTG GTAAACTGGA ATATGTCCTC 
CCTGTCTCTG TGTCTCCAAG CCTTCTGAAC CTGAACGAGA TGCGGATTTT TAAAGCTCAT 
AATTTAAAGC AAATATTTTA CAGTGGAGAA GGAGATGCAC TCACCAGAGA TGGCATCATC 
AAGTTCCCTA AGCTAAGAGA ATTGTATCTT TCAAATTGCA GCTTCTTTGG TCCAAAGAAT 
TTTACTGCCC AATTGCCTTC CTTGCAAATT CTAGAAATTG ATGGCCACAA AGAATTGGGA 
AATTTGTTTG CACAGCTCCA AGGGTTGACA AATTTGGAAA CATTACGCTT GCGCTCCCTG 
CTGGTGCCTG ATATTAGGTG TCTATGGAAG GGTCTCGTGT TGAGCAAATT GACTACTTTG 
AATGTGGTTG AGTGTAAAAG ACTGACGCAT GTATTCACAC GCAGTATGAT TGTCAGTCTA 
GTTCCACTGA AAGTTCTAAA GATATTGTCC TGTGAGGAAT TGGAGCAAAT CATTGCTAAG 
GATGATGATG AAAACGACCA GATATTGTTA GGAGATCATC TCCAATCTTT ATGCTTTCCC 
AATTTGTGTG AAATTGAGAT TAGAGAATGC AACAAGTTGA AGAGTCTCTT CCCATTAGCC 
ATGGCTTCAG GTCTCCCAAA TCTCCAAATA CTGAGGGTAA CAAAAGCCTC TCAATTATTG 
GGAGTCTTTG GGCAGGATGA TCATGCTTCA CCTGTCAACG TTGAGAAGGA GATGGTGCTT 
CCTAATCTAA AAAAGTTGTC TCTAGAACAA TTATCAAGTA TTGTCTGCTT CAGTTTCGGA 
TGCTGTGATT TCTTATTCCC TCGTTTGGAG AAGTTGAAGG TGTATGAATG CTCAAAGTTG 
ACCATAAAAT TTGCTATTAC ACCAAATGGT GGTTCAATGA GTGCTCAATC AGAGGTATCT 
CAACCAGCTG AAGATTCAAG CACTAATAGA GAGTGGACCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7459153 ( XM_024585984.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024441752.1 Populus trichocarpa probable disease resistance protein At5g63020 (LOC7459153), transcript variant X6, mRNA.

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CloneID	OPa422386
Clone ID Related Accession (Same CDS sequence)	XM_024585974.1 , XM_024585969.1 , XM_024585979.1 , XM_024585984.1 , XM_024585964.1
Accession Version	XM_024585984.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	probable disease resistance protein At5g63020 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTTG AAAGTGCTGG TGGATCCATT ATAGCTATGC TAGCAGAACT CATGGTGGAA 
CCAGTAGGAA GGCTGTTCCG TTACATGTTC TGTTTCAACA ATTTTGTTGA AGAATTCAAA 
GAACGAAAGG AGAACCTTGC TTTAGCACTA GATGGTCTGC AAAAAGAAGT CGAAGCTGCT 
GAAAGGAATG CTGAAGAAAT TAAGAAGGAT GTCAAAAAGT GGCTGGAAGA TGCAAAAAAC 
GAAATTGAAG GTGCGAAGCC ATTGGAAAAT GAAATAGGAA AAAATGGCAA ATGCTTTACT 
TGGTGCCCAA ACTGCATGCG ACAATTCACG TTAAGCAAGG CACTGGCCAA GAAGTCGGAG 
ACTTTCACAA AACTTGGAGA AACAAAGTTT ACAAAAGTGG CCGACGAACG TCCTCCTCAG 
CGCATAGAAT TTCTAACATC AAAGGAATTC ACGCCCTCAA AATCGTCAGA AGAAGCTTTG 
GAACAGATTA TGGAAGCTCT CAAAGATGAC AACGTCAATA TGATCGGACT GTACGGCATG 
GGAGGGGTGG GTAAAACCAC CTTGGTGAAA GAAGTAGGCA GGAGAGCAAA AGAGTTGCAG 
CTTTTTCCTG AAGTTTTGAT GGCTACGGTG TCCCAGAATC AAAATGTTAC AGACATCCAG 
GATCGAATGG CAGATAAGTT ATGTCTGGAT ATTAAAGAAA AGAGTAAAGA AGGGAGAGCA 
GATCGATTAT GGCAGAGACT GAAGCAAGTG GAGAAGATGC TTATAATCCT GGATGATGTT 
TGGAAACATA TTGACTTGAA AGAGATAGGG ATCCCATTTG GTGATGATCA CAGGGGTTGT 
AAAATTCTTC TAACAACACG TCGTCGAGAC ATATGTTCTT ATATGGTGTG CCAGAAAAAT 
GTGTTTCTAC GTCTCTTTTC TGAAAAAGAA GCATGGGATT TATTCAGAAT CAATGCAGGT 
TTAGATGATG GGGACTCTAC CTTGAACAGA GTGGCAAGGG ATGTTGCGAG AGAATGCCAC 
GGCTTGCCTA TAGCACTTGT GACAATGGGA AGGGCTCTAA GAGATGAATC TGCAGTTAAG 
TGGAAAAGAG TGTCTAAACA GCTCAAAAAC TCTCAATTTC CGGACATGGA ACAAATTGAA 
GAAAAAAATG CATATGCATG TCTTAAGTTG AGCTACGATT ATTTGAAGTC CAAGGAAACC 
AAGTTATGTT TCTTGCTATG CTGTTTATTT CCAGAAGATT ACAACATTCC AGTCGAGGAC 
TTGACGAGAT ATGCAGTTGG CTATGGGTTA CATCAAGATG GGGAGCCCAT TGAAGATGCA 
AGGGAACAAG TTCATGTGGC AATCCAAGAC CTCAAATCTT GCTGTTTGTT GTTGGGCACT 
GAAACTGAAG AACATGTGAG AATGCATGAC TTGGTTCGTG ATGTTGCTAT TCAGATAGCA 
TCATCAAAAG AATATGGATT CATGGTATTG GAGAAGTGGC CATTGAGTAT TGAAAGCTTT 
GAAGCTTGTA CAACAATTTC GTTAATGGGC AATAAACTAA CCGAACTTCC TGAAGGATTG 
GAATGTCCAC AGCTCAAAGT TCTATTGTTA GAATTGGATT ATGGTTTGTA TGTTCCTCAG 
AGGTTCTTTG AAGGGATGAA AGAAATTGAA GTTTTGTCTC TGGAGGGAGG GTGTTTGTCA 
TTGCAATCAC TTGAACTCTC AACGAAACTT CAATCGTTGG TGTTGATCAG GTGTGAATGC 
AAGGACCTCA TTTGGTTGAG AAAGCTGCAG AGACTTAAGA TTCTTGTTTT TATACGGTGC 
TTATCCATTG AAGAATTACC TGATGAAATA GGAGAGCTCA AGGAGTTAAG GTTGTTGGAT 
GTGACAGGTT GTCAAAGGCT AAGAAGGATC CCTGTGAATT TGATTGGAAG GTTGAAGAAG 
TTAGAAGAGC TGTTGATCGG GCATCGCAGC TTTGATGGAT GGGATGTTGT TGGATGTGAC 
AGCACCGGAG GAATGAATGC AAGCCTGACA GAACTAAATT CGTTGTCTCA GTTAGCCGTA 
TTATCATTGA GGATCCCGAA GGTTGAATGC ACTCCCAGAG ATTTTGTTTT TCCAGTTAGC 
TTGCGCAAAT ATGATATAAT ATTAGGGAAT CCGTTTGTGG CAGCCAATTA TGGATACCCA 
ACCTCGACAA GATTAATTTT GGCTGGTACA TCCTTAAATG CGAAGACATT TGAGCAATTG 
TTTTTACATA AATTAGAATT TGTAGAAGTG ACGCATTGTG GGGATGTTTT CACTCTGTTT 
CCAGCAAGAT TGCGGCAAGG TTTAAAAAAT CTGAAGGAGG TGATTGTTGA CAGCTGTAAA 
TCATTGGAAG AGGTGTTTGA ATTGGGTGAG GCTGATGAAG GAAGCAGTGA GGAGAAGGAG 
CTGCCGCTGC TGTCATCTTT AACAGAGTTA AAGCTGCGAT GGTTACCTGA GCTCAAATGC 
ATATGGCCCA CCGGACATAT CAGCCTCCAA AATCTTGCTC ATCTGGAGTT GTTTTCTCTC 
CACAAACTGA CATTTATCTT CACACCGCCC CTCGCTCGAA GTTTGTCAAA GCTAGAAGGC 
CTTTACATAA GAAATTGCGG TGAATTGAAG CATATTATCA GAGAAGAGGA AGGTGAAAGG 
GAAATAATTC CAGAGTCTCC TTGCTTCCCA GAATTAAAAA ATATCAACAT ATATTTCTGT 
GATAAACTGG AATATGTCTT GCCAGTCTCT TTATCTCATG ACAGAGATGG CATCATCAAG 
TTCCCTCAAC TAAGAGAATT GTCTCTTAAG CTCGGATCAA ATTACAGCTT TTTAGGTCCA 
AGGAATTTTG ATGCTCAATT GCCTTTGCAA GAATTAACCA TTAAAGGCCA CGAAGAAGTG 
GGTAATTGGT TGGCACAGCT ACAACAAAAC GGCTTCTTAC AAAGATTAGA ATATGTACAC 
GTGGACGATT GTGCGGATGT TCGCACTCCG TTTCCAGCAA AATTGCTGCG AGCTTTGAAA 
AATCTAAGGA TGGTGAACAT ATACGGCTGC AAATCATTGG AAGAGGTATT TGAATTAGGT 
GAGGCTGATG AAGGAAGCAG TGAGGAGAAG GAGCTGCCGC TGCTGTCATC TTTAACAGAG 
TTACAGCTGC AAAGGTTACC TGAGCTCAAA TGCATATGGA AGGGGCCCAC CAGAAATGTC 
AGCCTCCAAA GTCTTGTTCA TCTGAATGTT TGGTGTCTCA ACAAACTGAC ATTTATCTTC 
ACATCATCCC TCGCGCAGAG TCTGCCAAAG CTAGAAAGAC TTTCCATGAG TGATTGCGGT 
GAATTGAAGC ATATTATCAG AGAAGAGGAT GGTGAAAGGG AAATAATTCC AGAGTCTCCT 
GGCTTCCCAA AATTAAAAAC TATCAGCATA TCTGACTGTG GTAAACTGGA ATATGTCCTC 
CCTGTCTCTG TGTCTCCAAG CCTTCTGAAC CTGAACGAGA TGCGGATTTT TAAAGCTCAT 
AATTTAAAGC AAATATTTTA CAGTGGAGAA GGAGATGCAC TCACCAGAGA TGGCATCATC 
AAGTTCCCTA AGCTAAGAGA ATTGTATCTT TCAAATTGCA GCTTCTTTGG TCCAAAGAAT 
TTTACTGCCC AATTGCCTTC CTTGCAAATT CTAGAAATTG ATGGCCACAA AGAATTGGGA 
AATTTGTTTG CACAGCTCCA AGGGTTGACA AATTTGGAAA CATTACGCTT GCGCTCCCTG 
CTGGTGCCTG ATATTAGGTG TCTATGGAAG GGTCTCGTGT TGAGCAAATT GACTACTTTG 
AATGTGGTTG AGTGTAAAAG ACTGACGCAT GTATTCACAC GCAGTATGAT TGTCAGTCTA 
GTTCCACTGA AAGTTCTAAA GATATTGTCC TGTGAGGAAT TGGAGCAAAT CATTGCTAAG 
GATGATGATG AAAACGACCA GATATTGTTA GGAGATCATC TCCAATCTTT ATGCTTTCCC 
AATTTGTGTG AAATTGAGAT TAGAGAATGC AACAAGTTGA AGAGTCTCTT CCCATTAGCC 
ATGGCTTCAG GTCTCCCAAA TCTCCAAATA CTGAGGGTAA CAAAAGCCTC TCAATTATTG 
GGAGTCTTTG GGCAGGATGA TCATGCTTCA CCTGTCAACG TTGAGAAGGA GATGGTGCTT 
CCTAATCTAA AAAAGTTGTC TCTAGAACAA TTATCAAGTA TTGTCTGCTT CAGTTTCGGA 
TGCTGTGATT TCTTATTCCC TCGTTTGGAG AAGTTGAAGG TGTATGAATG CTCAAAGTTG 
ACCATAAAAT TTGCTATTAC ACCAAATGGT GGTTCAATGA GTGCTCAATC AGAGGTATCT 
CAACCAGCTG AAGATTCAAG CACTAATAGA GAGTGGACCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024441752.1
CDS	1755..6119
Translation	MALESAGGSIIAMLAELMVEPVGRLFRYMFCFNNFVEEFKERKENLALALDGLQKEVEAAERNAEEIKKDVKKWLEDAKNEIEGAKPLENEIGKNGKCFTWCPNCMRQFTLSKALAKKSETFTKLGETKFTKVADERPPQRIEFLTSKEFTPSKSSEEALEQIMEALKDDNVNMIGLYGMGGVGKTTLVKEVGRRAKELQLFPEVLMATVSQNQNVTDIQDRMADKLCLDIKEKSKEGRADRLWQRLKQVEKMLIILDDVWKHIDLKEIGIPFGDDHRGCKILLTTRRRDICSYMVCQKNVFLRLFSEKEAWDLFRINAGLDDGDSTLNRVARDVARECHGLPIALVTMGRALRDESAVKWKRVSKQLKNSQFPDMEQIEEKNAYACLKLSYDYLKSKETKLCFLLCCLFPEDYNIPVEDLTRYAVGYGLHQDGEPIEDAREQVHVAIQDLKSCCLLLGTETEEHVRMHDLVRDVAIQIASSKEYGFMVLEKWPLSIESFEACTTISLMGNKLTELPEGLECPQLKVLLLELDYGLYVPQRFFEGMKEIEVLSLEGGCLSLQSLELSTKLQSLVLIRCECKDLIWLRKLQRLKILVFIRCLSIEELPDEIGELKELRLLDVTGCQRLRRIPVNLIGRLKKLEELLIGHRSFDGWDVVGCDSTGGMNASLTELNSLSQLAVLSLRIPKVECTPRDFVFPVSLRKYDIILGNPFVAANYGYPTSTRLILAGTSLNAKTFEQLFLHKLEFVEVTHCGDVFTLFPARLRQGLKNLKEVIVDSCKSLEEVFELGEADEGSSEEKELPLLSSLTELKLRWLPELKCIWPTGHISLQNLAHLELFSLHKLTFIFTPPLARSLSKLEGLYIRNCGELKHIIREEEGEREIIPESPCFPELKNINIYFCDKLEYVLPVSLSHDRDGIIKFPQLRELSLKLGSNYSFLGPRNFDAQLPLQELTIKGHEEVGNWLAQLQQNGFLQRLEYVHVDDCADVRTPFPAKLLRALKNLRMVNIYGCKSLEEVFELGEADEGSSEEKELPLLSSLTELQLQRLPELKCIWKGPTRNVSLQSLVHLNVWCLNKLTFIFTSSLAQSLPKLERLSMSDCGELKHIIREEDGEREIIPESPGFPKLKTISISDCGKLEYVLPVSVSPSLLNLNEMRIFKAHNLKQIFYSGEGDALTRDGIIKFPKLRELYLSNCSFFGPKNFTAQLPSLQILEIDGHKELGNLFAQLQGLTNLETLRLRSLLVPDIRCLWKGLVLSKLTTLNVVECKRLTHVFTRSMIVSLVPLKVLKILSCEELEQIIAKDDDENDQILLGDHLQSLCFPNLCEIEIRECNKLKSLFPLAMASGLPNLQILRVTKASQLLGVFGQDDHASPVNVEKEMVLPNLKKLSLEQLSSIVCFSFGCCDFLFPRLEKLKVYECSKLTIKFAITPNGGSMSAQSEVSQPAEDSSTNREWTS

Target ORF information:

RefSeq Version	XM_024585984.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa probable disease resistance protein At5g63020 (LOC7459153), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024585984.1

ORF Insert Sequence:

ATGGCTCTTG AAAGTGCTGG TGGATCCATT ATAGCTATGC TAGCAGAACT CATGGTGGAA 
CCAGTAGGAA GGCTGTTCCG TTACATGTTC TGTTTCAACA ATTTTGTTGA AGAATTCAAA 
GAACGAAAGG AGAACCTTGC TTTAGCACTA GATGGTCTGC AAAAAGAAGT CGAAGCTGCT 
GAAAGGAATG CTGAAGAAAT TAAGAAGGAT GTCAAAAAGT GGCTGGAAGA TGCAAAAAAC 
GAAATTGAAG GTGCGAAGCC ATTGGAAAAT GAAATAGGAA AAAATGGCAA ATGCTTTACT 
TGGTGCCCAA ACTGCATGCG ACAATTCACG TTAAGCAAGG CACTGGCCAA GAAGTCGGAG 
ACTTTCACAA AACTTGGAGA AACAAAGTTT ACAAAAGTGG CCGACGAACG TCCTCCTCAG 
CGCATAGAAT TTCTAACATC AAAGGAATTC ACGCCCTCAA AATCGTCAGA AGAAGCTTTG 
GAACAGATTA TGGAAGCTCT CAAAGATGAC AACGTCAATA TGATCGGACT GTACGGCATG 
GGAGGGGTGG GTAAAACCAC CTTGGTGAAA GAAGTAGGCA GGAGAGCAAA AGAGTTGCAG 
CTTTTTCCTG AAGTTTTGAT GGCTACGGTG TCCCAGAATC AAAATGTTAC AGACATCCAG 
GATCGAATGG CAGATAAGTT ATGTCTGGAT ATTAAAGAAA AGAGTAAAGA AGGGAGAGCA 
GATCGATTAT GGCAGAGACT GAAGCAAGTG GAGAAGATGC TTATAATCCT GGATGATGTT 
TGGAAACATA TTGACTTGAA AGAGATAGGG ATCCCATTTG GTGATGATCA CAGGGGTTGT 
AAAATTCTTC TAACAACACG TCGTCGAGAC ATATGTTCTT ATATGGTGTG CCAGAAAAAT 
GTGTTTCTAC GTCTCTTTTC TGAAAAAGAA GCATGGGATT TATTCAGAAT CAATGCAGGT 
TTAGATGATG GGGACTCTAC CTTGAACAGA GTGGCAAGGG ATGTTGCGAG AGAATGCCAC 
GGCTTGCCTA TAGCACTTGT GACAATGGGA AGGGCTCTAA GAGATGAATC TGCAGTTAAG 
TGGAAAAGAG TGTCTAAACA GCTCAAAAAC TCTCAATTTC CGGACATGGA ACAAATTGAA 
GAAAAAAATG CATATGCATG TCTTAAGTTG AGCTACGATT ATTTGAAGTC CAAGGAAACC 
AAGTTATGTT TCTTGCTATG CTGTTTATTT CCAGAAGATT ACAACATTCC AGTCGAGGAC 
TTGACGAGAT ATGCAGTTGG CTATGGGTTA CATCAAGATG GGGAGCCCAT TGAAGATGCA 
AGGGAACAAG TTCATGTGGC AATCCAAGAC CTCAAATCTT GCTGTTTGTT GTTGGGCACT 
GAAACTGAAG AACATGTGAG AATGCATGAC TTGGTTCGTG ATGTTGCTAT TCAGATAGCA 
TCATCAAAAG AATATGGATT CATGGTATTG GAGAAGTGGC CATTGAGTAT TGAAAGCTTT 
GAAGCTTGTA CAACAATTTC GTTAATGGGC AATAAACTAA CCGAACTTCC TGAAGGATTG 
GAATGTCCAC AGCTCAAAGT TCTATTGTTA GAATTGGATT ATGGTTTGTA TGTTCCTCAG 
AGGTTCTTTG AAGGGATGAA AGAAATTGAA GTTTTGTCTC TGGAGGGAGG GTGTTTGTCA 
TTGCAATCAC TTGAACTCTC AACGAAACTT CAATCGTTGG TGTTGATCAG GTGTGAATGC 
AAGGACCTCA TTTGGTTGAG AAAGCTGCAG AGACTTAAGA TTCTTGTTTT TATACGGTGC 
TTATCCATTG AAGAATTACC TGATGAAATA GGAGAGCTCA AGGAGTTAAG GTTGTTGGAT 
GTGACAGGTT GTCAAAGGCT AAGAAGGATC CCTGTGAATT TGATTGGAAG GTTGAAGAAG 
TTAGAAGAGC TGTTGATCGG GCATCGCAGC TTTGATGGAT GGGATGTTGT TGGATGTGAC 
AGCACCGGAG GAATGAATGC AAGCCTGACA GAACTAAATT CGTTGTCTCA GTTAGCCGTA 
TTATCATTGA GGATCCCGAA GGTTGAATGC ACTCCCAGAG ATTTTGTTTT TCCAGTTAGC 
TTGCGCAAAT ATGATATAAT ATTAGGGAAT CCGTTTGTGG CAGCCAATTA TGGATACCCA 
ACCTCGACAA GATTAATTTT GGCTGGTACA TCCTTAAATG CGAAGACATT TGAGCAATTG 
TTTTTACATA AATTAGAATT TGTAGAAGTG ACGCATTGTG GGGATGTTTT CACTCTGTTT 
CCAGCAAGAT TGCGGCAAGG TTTAAAAAAT CTGAAGGAGG TGATTGTTGA CAGCTGTAAA 
TCATTGGAAG AGGTGTTTGA ATTGGGTGAG GCTGATGAAG GAAGCAGTGA GGAGAAGGAG 
CTGCCGCTGC TGTCATCTTT AACAGAGTTA AAGCTGCGAT GGTTACCTGA GCTCAAATGC 
ATATGGCCCA CCGGACATAT CAGCCTCCAA AATCTTGCTC ATCTGGAGTT GTTTTCTCTC 
CACAAACTGA CATTTATCTT CACACCGCCC CTCGCTCGAA GTTTGTCAAA GCTAGAAGGC 
CTTTACATAA GAAATTGCGG TGAATTGAAG CATATTATCA GAGAAGAGGA AGGTGAAAGG 
GAAATAATTC CAGAGTCTCC TTGCTTCCCA GAATTAAAAA ATATCAACAT ATATTTCTGT 
GATAAACTGG AATATGTCTT GCCAGTCTCT TTATCTCATG ACAGAGATGG CATCATCAAG 
TTCCCTCAAC TAAGAGAATT GTCTCTTAAG CTCGGATCAA ATTACAGCTT TTTAGGTCCA 
AGGAATTTTG ATGCTCAATT GCCTTTGCAA GAATTAACCA TTAAAGGCCA CGAAGAAGTG 
GGTAATTGGT TGGCACAGCT ACAACAAAAC GGCTTCTTAC AAAGATTAGA ATATGTACAC 
GTGGACGATT GTGCGGATGT TCGCACTCCG TTTCCAGCAA AATTGCTGCG AGCTTTGAAA 
AATCTAAGGA TGGTGAACAT ATACGGCTGC AAATCATTGG AAGAGGTATT TGAATTAGGT 
GAGGCTGATG AAGGAAGCAG TGAGGAGAAG GAGCTGCCGC TGCTGTCATC TTTAACAGAG 
TTACAGCTGC AAAGGTTACC TGAGCTCAAA TGCATATGGA AGGGGCCCAC CAGAAATGTC 
AGCCTCCAAA GTCTTGTTCA TCTGAATGTT TGGTGTCTCA ACAAACTGAC ATTTATCTTC 
ACATCATCCC TCGCGCAGAG TCTGCCAAAG CTAGAAAGAC TTTCCATGAG TGATTGCGGT 
GAATTGAAGC ATATTATCAG AGAAGAGGAT GGTGAAAGGG AAATAATTCC AGAGTCTCCT 
GGCTTCCCAA AATTAAAAAC TATCAGCATA TCTGACTGTG GTAAACTGGA ATATGTCCTC 
CCTGTCTCTG TGTCTCCAAG CCTTCTGAAC CTGAACGAGA TGCGGATTTT TAAAGCTCAT 
AATTTAAAGC AAATATTTTA CAGTGGAGAA GGAGATGCAC TCACCAGAGA TGGCATCATC 
AAGTTCCCTA AGCTAAGAGA ATTGTATCTT TCAAATTGCA GCTTCTTTGG TCCAAAGAAT 
TTTACTGCCC AATTGCCTTC CTTGCAAATT CTAGAAATTG ATGGCCACAA AGAATTGGGA 
AATTTGTTTG CACAGCTCCA AGGGTTGACA AATTTGGAAA CATTACGCTT GCGCTCCCTG 
CTGGTGCCTG ATATTAGGTG TCTATGGAAG GGTCTCGTGT TGAGCAAATT GACTACTTTG 
AATGTGGTTG AGTGTAAAAG ACTGACGCAT GTATTCACAC GCAGTATGAT TGTCAGTCTA 
GTTCCACTGA AAGTTCTAAA GATATTGTCC TGTGAGGAAT TGGAGCAAAT CATTGCTAAG 
GATGATGATG AAAACGACCA GATATTGTTA GGAGATCATC TCCAATCTTT ATGCTTTCCC 
AATTTGTGTG AAATTGAGAT TAGAGAATGC AACAAGTTGA AGAGTCTCTT CCCATTAGCC 
ATGGCTTCAG GTCTCCCAAA TCTCCAAATA CTGAGGGTAA CAAAAGCCTC TCAATTATTG 
GGAGTCTTTG GGCAGGATGA TCATGCTTCA CCTGTCAACG TTGAGAAGGA GATGGTGCTT 
CCTAATCTAA AAAAGTTGTC TCTAGAACAA TTATCAAGTA TTGTCTGCTT CAGTTTCGGA 
TGCTGTGATT TCTTATTCCC TCGTTTGGAG AAGTTGAAGG TGTATGAATG CTCAAAGTTG 
ACCATAAAAT TTGCTATTAC ACCAAATGGT GGTTCAATGA GTGCTCAATC AGAGGTATCT 
CAACCAGCTG AAGATTCAAG CACTAATAGA GAGTGGACCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7459153 ( XM_024585964.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024441732.1 Populus trichocarpa probable disease resistance protein At5g63020 (LOC7459153), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa422386
Clone ID Related Accession (Same CDS sequence)	XM_024585974.1 , XM_024585969.1 , XM_024585979.1 , XM_024585984.1 , XM_024585964.1
Accession Version	XM_024585964.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	probable disease resistance protein At5g63020 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTTG AAAGTGCTGG TGGATCCATT ATAGCTATGC TAGCAGAACT CATGGTGGAA 
CCAGTAGGAA GGCTGTTCCG TTACATGTTC TGTTTCAACA ATTTTGTTGA AGAATTCAAA 
GAACGAAAGG AGAACCTTGC TTTAGCACTA GATGGTCTGC AAAAAGAAGT CGAAGCTGCT 
GAAAGGAATG CTGAAGAAAT TAAGAAGGAT GTCAAAAAGT GGCTGGAAGA TGCAAAAAAC 
GAAATTGAAG GTGCGAAGCC ATTGGAAAAT GAAATAGGAA AAAATGGCAA ATGCTTTACT 
TGGTGCCCAA ACTGCATGCG ACAATTCACG TTAAGCAAGG CACTGGCCAA GAAGTCGGAG 
ACTTTCACAA AACTTGGAGA AACAAAGTTT ACAAAAGTGG CCGACGAACG TCCTCCTCAG 
CGCATAGAAT TTCTAACATC AAAGGAATTC ACGCCCTCAA AATCGTCAGA AGAAGCTTTG 
GAACAGATTA TGGAAGCTCT CAAAGATGAC AACGTCAATA TGATCGGACT GTACGGCATG 
GGAGGGGTGG GTAAAACCAC CTTGGTGAAA GAAGTAGGCA GGAGAGCAAA AGAGTTGCAG 
CTTTTTCCTG AAGTTTTGAT GGCTACGGTG TCCCAGAATC AAAATGTTAC AGACATCCAG 
GATCGAATGG CAGATAAGTT ATGTCTGGAT ATTAAAGAAA AGAGTAAAGA AGGGAGAGCA 
GATCGATTAT GGCAGAGACT GAAGCAAGTG GAGAAGATGC TTATAATCCT GGATGATGTT 
TGGAAACATA TTGACTTGAA AGAGATAGGG ATCCCATTTG GTGATGATCA CAGGGGTTGT 
AAAATTCTTC TAACAACACG TCGTCGAGAC ATATGTTCTT ATATGGTGTG CCAGAAAAAT 
GTGTTTCTAC GTCTCTTTTC TGAAAAAGAA GCATGGGATT TATTCAGAAT CAATGCAGGT 
TTAGATGATG GGGACTCTAC CTTGAACAGA GTGGCAAGGG ATGTTGCGAG AGAATGCCAC 
GGCTTGCCTA TAGCACTTGT GACAATGGGA AGGGCTCTAA GAGATGAATC TGCAGTTAAG 
TGGAAAAGAG TGTCTAAACA GCTCAAAAAC TCTCAATTTC CGGACATGGA ACAAATTGAA 
GAAAAAAATG CATATGCATG TCTTAAGTTG AGCTACGATT ATTTGAAGTC CAAGGAAACC 
AAGTTATGTT TCTTGCTATG CTGTTTATTT CCAGAAGATT ACAACATTCC AGTCGAGGAC 
TTGACGAGAT ATGCAGTTGG CTATGGGTTA CATCAAGATG GGGAGCCCAT TGAAGATGCA 
AGGGAACAAG TTCATGTGGC AATCCAAGAC CTCAAATCTT GCTGTTTGTT GTTGGGCACT 
GAAACTGAAG AACATGTGAG AATGCATGAC TTGGTTCGTG ATGTTGCTAT TCAGATAGCA 
TCATCAAAAG AATATGGATT CATGGTATTG GAGAAGTGGC CATTGAGTAT TGAAAGCTTT 
GAAGCTTGTA CAACAATTTC GTTAATGGGC AATAAACTAA CCGAACTTCC TGAAGGATTG 
GAATGTCCAC AGCTCAAAGT TCTATTGTTA GAATTGGATT ATGGTTTGTA TGTTCCTCAG 
AGGTTCTTTG AAGGGATGAA AGAAATTGAA GTTTTGTCTC TGGAGGGAGG GTGTTTGTCA 
TTGCAATCAC TTGAACTCTC AACGAAACTT CAATCGTTGG TGTTGATCAG GTGTGAATGC 
AAGGACCTCA TTTGGTTGAG AAAGCTGCAG AGACTTAAGA TTCTTGTTTT TATACGGTGC 
TTATCCATTG AAGAATTACC TGATGAAATA GGAGAGCTCA AGGAGTTAAG GTTGTTGGAT 
GTGACAGGTT GTCAAAGGCT AAGAAGGATC CCTGTGAATT TGATTGGAAG GTTGAAGAAG 
TTAGAAGAGC TGTTGATCGG GCATCGCAGC TTTGATGGAT GGGATGTTGT TGGATGTGAC 
AGCACCGGAG GAATGAATGC AAGCCTGACA GAACTAAATT CGTTGTCTCA GTTAGCCGTA 
TTATCATTGA GGATCCCGAA GGTTGAATGC ACTCCCAGAG ATTTTGTTTT TCCAGTTAGC 
TTGCGCAAAT ATGATATAAT ATTAGGGAAT CCGTTTGTGG CAGCCAATTA TGGATACCCA 
ACCTCGACAA GATTAATTTT GGCTGGTACA TCCTTAAATG CGAAGACATT TGAGCAATTG 
TTTTTACATA AATTAGAATT TGTAGAAGTG ACGCATTGTG GGGATGTTTT CACTCTGTTT 
CCAGCAAGAT TGCGGCAAGG TTTAAAAAAT CTGAAGGAGG TGATTGTTGA CAGCTGTAAA 
TCATTGGAAG AGGTGTTTGA ATTGGGTGAG GCTGATGAAG GAAGCAGTGA GGAGAAGGAG 
CTGCCGCTGC TGTCATCTTT AACAGAGTTA AAGCTGCGAT GGTTACCTGA GCTCAAATGC 
ATATGGCCCA CCGGACATAT CAGCCTCCAA AATCTTGCTC ATCTGGAGTT GTTTTCTCTC 
CACAAACTGA CATTTATCTT CACACCGCCC CTCGCTCGAA GTTTGTCAAA GCTAGAAGGC 
CTTTACATAA GAAATTGCGG TGAATTGAAG CATATTATCA GAGAAGAGGA AGGTGAAAGG 
GAAATAATTC CAGAGTCTCC TTGCTTCCCA GAATTAAAAA ATATCAACAT ATATTTCTGT 
GATAAACTGG AATATGTCTT GCCAGTCTCT TTATCTCATG ACAGAGATGG CATCATCAAG 
TTCCCTCAAC TAAGAGAATT GTCTCTTAAG CTCGGATCAA ATTACAGCTT TTTAGGTCCA 
AGGAATTTTG ATGCTCAATT GCCTTTGCAA GAATTAACCA TTAAAGGCCA CGAAGAAGTG 
GGTAATTGGT TGGCACAGCT ACAACAAAAC GGCTTCTTAC AAAGATTAGA ATATGTACAC 
GTGGACGATT GTGCGGATGT TCGCACTCCG TTTCCAGCAA AATTGCTGCG AGCTTTGAAA 
AATCTAAGGA TGGTGAACAT ATACGGCTGC AAATCATTGG AAGAGGTATT TGAATTAGGT 
GAGGCTGATG AAGGAAGCAG TGAGGAGAAG GAGCTGCCGC TGCTGTCATC TTTAACAGAG 
TTACAGCTGC AAAGGTTACC TGAGCTCAAA TGCATATGGA AGGGGCCCAC CAGAAATGTC 
AGCCTCCAAA GTCTTGTTCA TCTGAATGTT TGGTGTCTCA ACAAACTGAC ATTTATCTTC 
ACATCATCCC TCGCGCAGAG TCTGCCAAAG CTAGAAAGAC TTTCCATGAG TGATTGCGGT 
GAATTGAAGC ATATTATCAG AGAAGAGGAT GGTGAAAGGG AAATAATTCC AGAGTCTCCT 
GGCTTCCCAA AATTAAAAAC TATCAGCATA TCTGACTGTG GTAAACTGGA ATATGTCCTC 
CCTGTCTCTG TGTCTCCAAG CCTTCTGAAC CTGAACGAGA TGCGGATTTT TAAAGCTCAT 
AATTTAAAGC AAATATTTTA CAGTGGAGAA GGAGATGCAC TCACCAGAGA TGGCATCATC 
AAGTTCCCTA AGCTAAGAGA ATTGTATCTT TCAAATTGCA GCTTCTTTGG TCCAAAGAAT 
TTTACTGCCC AATTGCCTTC CTTGCAAATT CTAGAAATTG ATGGCCACAA AGAATTGGGA 
AATTTGTTTG CACAGCTCCA AGGGTTGACA AATTTGGAAA CATTACGCTT GCGCTCCCTG 
CTGGTGCCTG ATATTAGGTG TCTATGGAAG GGTCTCGTGT TGAGCAAATT GACTACTTTG 
AATGTGGTTG AGTGTAAAAG ACTGACGCAT GTATTCACAC GCAGTATGAT TGTCAGTCTA 
GTTCCACTGA AAGTTCTAAA GATATTGTCC TGTGAGGAAT TGGAGCAAAT CATTGCTAAG 
GATGATGATG AAAACGACCA GATATTGTTA GGAGATCATC TCCAATCTTT ATGCTTTCCC 
AATTTGTGTG AAATTGAGAT TAGAGAATGC AACAAGTTGA AGAGTCTCTT CCCATTAGCC 
ATGGCTTCAG GTCTCCCAAA TCTCCAAATA CTGAGGGTAA CAAAAGCCTC TCAATTATTG 
GGAGTCTTTG GGCAGGATGA TCATGCTTCA CCTGTCAACG TTGAGAAGGA GATGGTGCTT 
CCTAATCTAA AAAAGTTGTC TCTAGAACAA TTATCAAGTA TTGTCTGCTT CAGTTTCGGA 
TGCTGTGATT TCTTATTCCC TCGTTTGGAG AAGTTGAAGG TGTATGAATG CTCAAAGTTG 
ACCATAAAAT TTGCTATTAC ACCAAATGGT GGTTCAATGA GTGCTCAATC AGAGGTATCT 
CAACCAGCTG AAGATTCAAG CACTAATAGA GAGTGGACCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024441732.1
CDS	509..4873
Translation	MALESAGGSIIAMLAELMVEPVGRLFRYMFCFNNFVEEFKERKENLALALDGLQKEVEAAERNAEEIKKDVKKWLEDAKNEIEGAKPLENEIGKNGKCFTWCPNCMRQFTLSKALAKKSETFTKLGETKFTKVADERPPQRIEFLTSKEFTPSKSSEEALEQIMEALKDDNVNMIGLYGMGGVGKTTLVKEVGRRAKELQLFPEVLMATVSQNQNVTDIQDRMADKLCLDIKEKSKEGRADRLWQRLKQVEKMLIILDDVWKHIDLKEIGIPFGDDHRGCKILLTTRRRDICSYMVCQKNVFLRLFSEKEAWDLFRINAGLDDGDSTLNRVARDVARECHGLPIALVTMGRALRDESAVKWKRVSKQLKNSQFPDMEQIEEKNAYACLKLSYDYLKSKETKLCFLLCCLFPEDYNIPVEDLTRYAVGYGLHQDGEPIEDAREQVHVAIQDLKSCCLLLGTETEEHVRMHDLVRDVAIQIASSKEYGFMVLEKWPLSIESFEACTTISLMGNKLTELPEGLECPQLKVLLLELDYGLYVPQRFFEGMKEIEVLSLEGGCLSLQSLELSTKLQSLVLIRCECKDLIWLRKLQRLKILVFIRCLSIEELPDEIGELKELRLLDVTGCQRLRRIPVNLIGRLKKLEELLIGHRSFDGWDVVGCDSTGGMNASLTELNSLSQLAVLSLRIPKVECTPRDFVFPVSLRKYDIILGNPFVAANYGYPTSTRLILAGTSLNAKTFEQLFLHKLEFVEVTHCGDVFTLFPARLRQGLKNLKEVIVDSCKSLEEVFELGEADEGSSEEKELPLLSSLTELKLRWLPELKCIWPTGHISLQNLAHLELFSLHKLTFIFTPPLARSLSKLEGLYIRNCGELKHIIREEEGEREIIPESPCFPELKNINIYFCDKLEYVLPVSLSHDRDGIIKFPQLRELSLKLGSNYSFLGPRNFDAQLPLQELTIKGHEEVGNWLAQLQQNGFLQRLEYVHVDDCADVRTPFPAKLLRALKNLRMVNIYGCKSLEEVFELGEADEGSSEEKELPLLSSLTELQLQRLPELKCIWKGPTRNVSLQSLVHLNVWCLNKLTFIFTSSLAQSLPKLERLSMSDCGELKHIIREEDGEREIIPESPGFPKLKTISISDCGKLEYVLPVSVSPSLLNLNEMRIFKAHNLKQIFYSGEGDALTRDGIIKFPKLRELYLSNCSFFGPKNFTAQLPSLQILEIDGHKELGNLFAQLQGLTNLETLRLRSLLVPDIRCLWKGLVLSKLTTLNVVECKRLTHVFTRSMIVSLVPLKVLKILSCEELEQIIAKDDDENDQILLGDHLQSLCFPNLCEIEIRECNKLKSLFPLAMASGLPNLQILRVTKASQLLGVFGQDDHASPVNVEKEMVLPNLKKLSLEQLSSIVCFSFGCCDFLFPRLEKLKVYECSKLTIKFAITPNGGSMSAQSEVSQPAEDSSTNREWTS

Target ORF information:

RefSeq Version	XM_024585964.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa probable disease resistance protein At5g63020 (LOC7459153), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024585964.1

ORF Insert Sequence:

ATGGCTCTTG AAAGTGCTGG TGGATCCATT ATAGCTATGC TAGCAGAACT CATGGTGGAA 
CCAGTAGGAA GGCTGTTCCG TTACATGTTC TGTTTCAACA ATTTTGTTGA AGAATTCAAA 
GAACGAAAGG AGAACCTTGC TTTAGCACTA GATGGTCTGC AAAAAGAAGT CGAAGCTGCT 
GAAAGGAATG CTGAAGAAAT TAAGAAGGAT GTCAAAAAGT GGCTGGAAGA TGCAAAAAAC 
GAAATTGAAG GTGCGAAGCC ATTGGAAAAT GAAATAGGAA AAAATGGCAA ATGCTTTACT 
TGGTGCCCAA ACTGCATGCG ACAATTCACG TTAAGCAAGG CACTGGCCAA GAAGTCGGAG 
ACTTTCACAA AACTTGGAGA AACAAAGTTT ACAAAAGTGG CCGACGAACG TCCTCCTCAG 
CGCATAGAAT TTCTAACATC AAAGGAATTC ACGCCCTCAA AATCGTCAGA AGAAGCTTTG 
GAACAGATTA TGGAAGCTCT CAAAGATGAC AACGTCAATA TGATCGGACT GTACGGCATG 
GGAGGGGTGG GTAAAACCAC CTTGGTGAAA GAAGTAGGCA GGAGAGCAAA AGAGTTGCAG 
CTTTTTCCTG AAGTTTTGAT GGCTACGGTG TCCCAGAATC AAAATGTTAC AGACATCCAG 
GATCGAATGG CAGATAAGTT ATGTCTGGAT ATTAAAGAAA AGAGTAAAGA AGGGAGAGCA 
GATCGATTAT GGCAGAGACT GAAGCAAGTG GAGAAGATGC TTATAATCCT GGATGATGTT 
TGGAAACATA TTGACTTGAA AGAGATAGGG ATCCCATTTG GTGATGATCA CAGGGGTTGT 
AAAATTCTTC TAACAACACG TCGTCGAGAC ATATGTTCTT ATATGGTGTG CCAGAAAAAT 
GTGTTTCTAC GTCTCTTTTC TGAAAAAGAA GCATGGGATT TATTCAGAAT CAATGCAGGT 
TTAGATGATG GGGACTCTAC CTTGAACAGA GTGGCAAGGG ATGTTGCGAG AGAATGCCAC 
GGCTTGCCTA TAGCACTTGT GACAATGGGA AGGGCTCTAA GAGATGAATC TGCAGTTAAG 
TGGAAAAGAG TGTCTAAACA GCTCAAAAAC TCTCAATTTC CGGACATGGA ACAAATTGAA 
GAAAAAAATG CATATGCATG TCTTAAGTTG AGCTACGATT ATTTGAAGTC CAAGGAAACC 
AAGTTATGTT TCTTGCTATG CTGTTTATTT CCAGAAGATT ACAACATTCC AGTCGAGGAC 
TTGACGAGAT ATGCAGTTGG CTATGGGTTA CATCAAGATG GGGAGCCCAT TGAAGATGCA 
AGGGAACAAG TTCATGTGGC AATCCAAGAC CTCAAATCTT GCTGTTTGTT GTTGGGCACT 
GAAACTGAAG AACATGTGAG AATGCATGAC TTGGTTCGTG ATGTTGCTAT TCAGATAGCA 
TCATCAAAAG AATATGGATT CATGGTATTG GAGAAGTGGC CATTGAGTAT TGAAAGCTTT 
GAAGCTTGTA CAACAATTTC GTTAATGGGC AATAAACTAA CCGAACTTCC TGAAGGATTG 
GAATGTCCAC AGCTCAAAGT TCTATTGTTA GAATTGGATT ATGGTTTGTA TGTTCCTCAG 
AGGTTCTTTG AAGGGATGAA AGAAATTGAA GTTTTGTCTC TGGAGGGAGG GTGTTTGTCA 
TTGCAATCAC TTGAACTCTC AACGAAACTT CAATCGTTGG TGTTGATCAG GTGTGAATGC 
AAGGACCTCA TTTGGTTGAG AAAGCTGCAG AGACTTAAGA TTCTTGTTTT TATACGGTGC 
TTATCCATTG AAGAATTACC TGATGAAATA GGAGAGCTCA AGGAGTTAAG GTTGTTGGAT 
GTGACAGGTT GTCAAAGGCT AAGAAGGATC CCTGTGAATT TGATTGGAAG GTTGAAGAAG 
TTAGAAGAGC TGTTGATCGG GCATCGCAGC TTTGATGGAT GGGATGTTGT TGGATGTGAC 
AGCACCGGAG GAATGAATGC AAGCCTGACA GAACTAAATT CGTTGTCTCA GTTAGCCGTA 
TTATCATTGA GGATCCCGAA GGTTGAATGC ACTCCCAGAG ATTTTGTTTT TCCAGTTAGC 
TTGCGCAAAT ATGATATAAT ATTAGGGAAT CCGTTTGTGG CAGCCAATTA TGGATACCCA 
ACCTCGACAA GATTAATTTT GGCTGGTACA TCCTTAAATG CGAAGACATT TGAGCAATTG 
TTTTTACATA AATTAGAATT TGTAGAAGTG ACGCATTGTG GGGATGTTTT CACTCTGTTT 
CCAGCAAGAT TGCGGCAAGG TTTAAAAAAT CTGAAGGAGG TGATTGTTGA CAGCTGTAAA 
TCATTGGAAG AGGTGTTTGA ATTGGGTGAG GCTGATGAAG GAAGCAGTGA GGAGAAGGAG 
CTGCCGCTGC TGTCATCTTT AACAGAGTTA AAGCTGCGAT GGTTACCTGA GCTCAAATGC 
ATATGGCCCA CCGGACATAT CAGCCTCCAA AATCTTGCTC ATCTGGAGTT GTTTTCTCTC 
CACAAACTGA CATTTATCTT CACACCGCCC CTCGCTCGAA GTTTGTCAAA GCTAGAAGGC 
CTTTACATAA GAAATTGCGG TGAATTGAAG CATATTATCA GAGAAGAGGA AGGTGAAAGG 
GAAATAATTC CAGAGTCTCC TTGCTTCCCA GAATTAAAAA ATATCAACAT ATATTTCTGT 
GATAAACTGG AATATGTCTT GCCAGTCTCT TTATCTCATG ACAGAGATGG CATCATCAAG 
TTCCCTCAAC TAAGAGAATT GTCTCTTAAG CTCGGATCAA ATTACAGCTT TTTAGGTCCA 
AGGAATTTTG ATGCTCAATT GCCTTTGCAA GAATTAACCA TTAAAGGCCA CGAAGAAGTG 
GGTAATTGGT TGGCACAGCT ACAACAAAAC GGCTTCTTAC AAAGATTAGA ATATGTACAC 
GTGGACGATT GTGCGGATGT TCGCACTCCG TTTCCAGCAA AATTGCTGCG AGCTTTGAAA 
AATCTAAGGA TGGTGAACAT ATACGGCTGC AAATCATTGG AAGAGGTATT TGAATTAGGT 
GAGGCTGATG AAGGAAGCAG TGAGGAGAAG GAGCTGCCGC TGCTGTCATC TTTAACAGAG 
TTACAGCTGC AAAGGTTACC TGAGCTCAAA TGCATATGGA AGGGGCCCAC CAGAAATGTC 
AGCCTCCAAA GTCTTGTTCA TCTGAATGTT TGGTGTCTCA ACAAACTGAC ATTTATCTTC 
ACATCATCCC TCGCGCAGAG TCTGCCAAAG CTAGAAAGAC TTTCCATGAG TGATTGCGGT 
GAATTGAAGC ATATTATCAG AGAAGAGGAT GGTGAAAGGG AAATAATTCC AGAGTCTCCT 
GGCTTCCCAA AATTAAAAAC TATCAGCATA TCTGACTGTG GTAAACTGGA ATATGTCCTC 
CCTGTCTCTG TGTCTCCAAG CCTTCTGAAC CTGAACGAGA TGCGGATTTT TAAAGCTCAT 
AATTTAAAGC AAATATTTTA CAGTGGAGAA GGAGATGCAC TCACCAGAGA TGGCATCATC 
AAGTTCCCTA AGCTAAGAGA ATTGTATCTT TCAAATTGCA GCTTCTTTGG TCCAAAGAAT 
TTTACTGCCC AATTGCCTTC CTTGCAAATT CTAGAAATTG ATGGCCACAA AGAATTGGGA 
AATTTGTTTG CACAGCTCCA AGGGTTGACA AATTTGGAAA CATTACGCTT GCGCTCCCTG 
CTGGTGCCTG ATATTAGGTG TCTATGGAAG GGTCTCGTGT TGAGCAAATT GACTACTTTG 
AATGTGGTTG AGTGTAAAAG ACTGACGCAT GTATTCACAC GCAGTATGAT TGTCAGTCTA 
GTTCCACTGA AAGTTCTAAA GATATTGTCC TGTGAGGAAT TGGAGCAAAT CATTGCTAAG 
GATGATGATG AAAACGACCA GATATTGTTA GGAGATCATC TCCAATCTTT ATGCTTTCCC 
AATTTGTGTG AAATTGAGAT TAGAGAATGC AACAAGTTGA AGAGTCTCTT CCCATTAGCC 
ATGGCTTCAG GTCTCCCAAA TCTCCAAATA CTGAGGGTAA CAAAAGCCTC TCAATTATTG 
GGAGTCTTTG GGCAGGATGA TCATGCTTCA CCTGTCAACG TTGAGAAGGA GATGGTGCTT 
CCTAATCTAA AAAAGTTGTC TCTAGAACAA TTATCAAGTA TTGTCTGCTT CAGTTTCGGA 
TGCTGTGATT TCTTATTCCC TCGTTTGGAG AAGTTGAAGG TGTATGAATG CTCAAAGTTG 
ACCATAAAAT TTGCTATTAC ACCAAATGGT GGTTCAATGA GTGCTCAATC AGAGGTATCT 
CAACCAGCTG AAGATTCAAG CACTAATAGA GAGTGGACCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7459153 ( XM_024585987.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024441755.1 Populus trichocarpa probable disease resistance protein At5g63020 (LOC7459153), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OPa422387
Clone ID Related Accession (Same CDS sequence)	XM_024585987.1
Accession Version	XM_024585987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	disease resistance protein At4g27190 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACAAT TCACGTTAAG CAAGGCACTG GCCAAGAAGT CGGAGACTTT CACAAAACTT 
GGAGAAACAA AGTTTACAAA AGTGGCCGAC GAACGTCCTC CTCAGCGCAT AGAATTTCTA 
ACATCAAAGG AATTCACGCC CTCAAAATCG TCAGAAGAAG CTTTGGAACA GATTATGGAA 
GCTCTCAAAG ATGACAACGT CAATATGATC GGACTGTACG GCATGGGAGG GGTGGGTAAA 
ACCACCTTGG TGAAAGAAGT AGGCAGGAGA GCAAAAGAGT TGCAGCTTTT TCCTGAAGTT 
TTGATGGCTA CGGTGTCCCA GAATCAAAAT GTTACAGACA TCCAGGATCG AATGGCAGAT 
AAGTTATGTC TGGATATTAA AGAAAAGAGT AAAGAAGGGA GAGCAGATCG ATTATGGCAG 
AGACTGAAGC AAGTGGAGAA GATGCTTATA ATCCTGGATG ATGTTTGGAA ACATATTGAC 
TTGAAAGAGA TAGGGATCCC ATTTGGTGAT GATCACAGGG GTTGTAAAAT TCTTCTAACA 
ACACGTCGTC GAGACATATG TTCTTATATG GTGTGCCAGA AAAATGTGTT TCTACGTCTC 
TTTTCTGAAA AAGAAGCATG GGATTTATTC AGAATCAATG CAGGTTTAGA TGATGGGGAC 
TCTACCTTGA ACAGAGTGGC AAGGGATGTT GCGAGAGAAT GCCACGGCTT GCCTATAGCA 
CTTGTGACAA TGGGAAGGGC TCTAAGAGAT GAATCTGCAG TTAAGTGGAA AAGAGTGTCT 
AAACAGCTCA AAAACTCTCA ATTTCCGGAC ATGGAACAAA TTGAAGAAAA AAATGCATAT 
GCATGTCTTA AGTTGAGCTA CGATTATTTG AAGTCCAAGG AAACCAAGTT ATGTTTCTTG 
CTATGCTGTT TATTTCCAGA AGATTACAAC ATTCCAGTCG AGGACTTGAC GAGATATGCA 
GTTGGCTATG GGTTACATCA AGATGGGGAG CCCATTGAAG ATGCAAGGGA ACAAGTTCAT 
GTGGCAATCC AAGACCTCAA ATCTTGCTGT TTGTTGTTGG GCACTGAAAC TGAAGAACAT 
GTGAGAATGC ATGACTTGGT TCGTGATGTT GCTATTCAGA TAGCATCATC AAAAGAATAT 
GGATTCATGG TATTGGAGAA GTGGCCATTG AGTATTGAAA GCTTTGAAGC TTGTACAACA 
ATTTCGTTAA TGGGCAATAA ACTAACCGAA CTTCCTGAAG GATTGGAATG TCCACAGCTC 
AAAGTTCTAT TGTTAGAATT GGATTATGGT TTGTATGTTC CTCAGAGGTT CTTTGAAGGG 
ATGAAAGAAA TTGAAGTTTT GTCTCTGGAG GGAGGGTGTT TGTCATTGCA ATCACTTGAA 
CTCTCAACGA AACTTCAATC GTTGGTGTTG ATCAGGTGTG AATGCAAGGA CCTCATTTGG 
TTGAGAAAGC TGCAGAGACT TAAGATTCTT GTTTTTATAC GGTGCTTATC CATTGAAGAA 
TTACCTGATG AAATAGGAGA GCTCAAGGAG TTAAGGTTGT TGGATGTGAC AGGTTGTCAA 
AGGCTAAGAA GGATCCCTGT GAATTTGATT GGAAGGTTGA AGAAGTTAGA AGAGCTGTTG 
ATCGGGCATC GCAGCTTTGA TGGATGGGAT GTTGTTGGAT GTGACAGCAC CGGAGGAATG 
AATGCAAGCC TGACAGAACT AAATTCGTTG TCTCAGTTAG CCGTATTATC ATTGAGGATC 
CCGAAGGTTG AATGCACTCC CAGAGATTTT GTTTTTCCAG TTAGCTTGCG CAAATATGAT 
ATAATATTAG GGAATCCGTT TGTGGCAGCC AATTATGGAT ACCCAACCTC GACAAGATTA 
ATTTTGGCTG GTACATCCTT AAATGCGAAG ACATTTGAGC AATTGTTTTT ACATAAATTA 
GAATTTGTAG AAGTGACGCA TTGTGGGGAT GTTTTCACTC TGTTTCCAGC AAGATTGCGG 
CAAGGTTTAA AAAATCTGAA GGAGGTGATT GTTGACAGCT GTAAATCATT GGAAGAGGTG 
TTTGAATTGG GTGAGGCTGA TGAAGGAAGC AGTGAGGAGA AGGAGCTGCC GCTGCTGTCA 
TCTTTAACAG AGTTAAAGCT GCGATGGTTA CCTGAGCTCA AATGCATATG GCCCACCGGA 
CATATCAGCC TCCAAAATCT TGCTCATCTG GAGTTGTTTT CTCTCCACAA ACTGACATTT 
ATCTTCACAC CGCCCCTCGC TCGAAGTTTG TCAAAGCTAG AAGGCCTTTA CATAAGAAAT 
TGCGGTGAAT TGAAGCATAT TATCAGAGAA GAGGAAGGTG AAAGGGAAAT AATTCCAGAG 
TCTCCTTGCT TCCCAGAATT AAAAAATATC AACATATATT TCTGTGATAA ACTGGAATAT 
GTCTTGCCAG TCTCTTTATC TCATGACAGA GATGGCATCA TCAAGTTCCC TCAACTAAGA 
GAATTGTCTC TTAAGCTCGG ATCAAATTAC AGCTTTTTAG GTCCAAGGAA TTTTGATGCT 
CAATTGCCTT TGCAAGAATT AACCATTAAA GGCCACGAAG AAGTGGGTAA TTGGTTGGCA 
CAGCTACAAC AAAACGGCTT CTTACAAAGA TTAGAATATG TACACGTGGA CGATTGTGCG 
GATGTTCGCA CTCCGTTTCC AGCAAAATTG CTGCGAGCTT TGAAAAATCT AAGGATGGTG 
AACATATACG GCTGCAAATC ATTGGAAGAG GTATTTGAAT TAGGTGAGGC TGATGAAGGA 
AGCAGTGAGG AGAAGGAGCT GCCGCTGCTG TCATCTTTAA CAGAGTTACA GCTGCAAAGG 
TTACCTGAGC TCAAATGCAT ATGGAAGGGG CCCACCAGAA ATGTCAGCCT CCAAAGTCTT 
GTTCATCTGA ATGTTTGGTG TCTCAACAAA CTGACATTTA TCTTCACATC ATCCCTCGCG 
CAGAGTCTGC CAAAGCTAGA AAGACTTTCC ATGAGTGATT GCGGTGAATT GAAGCATATT 
ATCAGAGAAG AGGATGGTGA AAGGGAAATA ATTCCAGAGT CTCCTGGCTT CCCAAAATTA 
AAAACTATCA GCATATCTGA CTGTGGTAAA CTGGAATATG TCCTCCCTGT CTCTGTGTCT 
CCAAGCCTTC TGAACCTGAA CGAGATGCGG ATTTTTAAAG CTCATAATTT AAAGCAAATA 
TTTTACAGTG GAGAAGGAGA TGCACTCACC AGAGATGGCA TCATCAAGTT CCCTAAGCTA 
AGAGAATTGT ATCTTTCAAA TTGCAGCTTC TTTGGTCCAA AGAATTTTAC TGCCCAATTG 
CCTTCCTTGC AAATTCTAGA AATTGATGGC CACAAAGAAT TGGGAAATTT GTTTGCACAG 
CTCCAAGGGT TGACAAATTT GGAAACATTA CGCTTGCGCT CCCTGCTGGT GCCTGATATT 
AGGTGTCTAT GGAAGGGTCT CGTGTTGAGC AAATTGACTA CTTTGAATGT GGTTGAGTGT 
AAAAGACTGA CGCATGTATT CACACGCAGT ATGATTGTCA GTCTAGTTCC ACTGAAAGTT 
CTAAAGATAT TGTCCTGTGA GGAATTGGAG CAAATCATTG CTAAGGATGA TGATGAAAAC 
GACCAGATAT TGTTAGGAGA TCATCTCCAA TCTTTATGCT TTCCCAATTT GTGTGAAATT 
GAGATTAGAG AATGCAACAA GTTGAAGAGT CTCTTCCCAT TAGCCATGGC TTCAGGTCTC 
CCAAATCTCC AAATACTGAG GGTAACAAAA GCCTCTCAAT TATTGGGAGT CTTTGGGCAG 
GATGATCATG CTTCACCTGT CAACGTTGAG AAGGAGATGG TGCTTCCTAA TCTAAAAAAG 
TTGTCTCTAG AACAATTATC AAGTATTGTC TGCTTCAGTT TCGGATGCTG TGATTTCTTA 
TTCCCTCGTT TGGAGAAGTT GAAGGTGTAT GAATGCTCAA AGTTGACCAT AAAATTTGCT 
ATTACACCAA ATGGTGGTTC AATGAGTGCT CAATCAGAGG TATCTCAACC AGCTGAAGAT 
TCAAGCACTA ATAGAGAGTG GACCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024441755.1
CDS	135..4184
Translation	MRQFTLSKALAKKSETFTKLGETKFTKVADERPPQRIEFLTSKEFTPSKSSEEALEQIMEALKDDNVNMIGLYGMGGVGKTTLVKEVGRRAKELQLFPEVLMATVSQNQNVTDIQDRMADKLCLDIKEKSKEGRADRLWQRLKQVEKMLIILDDVWKHIDLKEIGIPFGDDHRGCKILLTTRRRDICSYMVCQKNVFLRLFSEKEAWDLFRINAGLDDGDSTLNRVARDVARECHGLPIALVTMGRALRDESAVKWKRVSKQLKNSQFPDMEQIEEKNAYACLKLSYDYLKSKETKLCFLLCCLFPEDYNIPVEDLTRYAVGYGLHQDGEPIEDAREQVHVAIQDLKSCCLLLGTETEEHVRMHDLVRDVAIQIASSKEYGFMVLEKWPLSIESFEACTTISLMGNKLTELPEGLECPQLKVLLLELDYGLYVPQRFFEGMKEIEVLSLEGGCLSLQSLELSTKLQSLVLIRCECKDLIWLRKLQRLKILVFIRCLSIEELPDEIGELKELRLLDVTGCQRLRRIPVNLIGRLKKLEELLIGHRSFDGWDVVGCDSTGGMNASLTELNSLSQLAVLSLRIPKVECTPRDFVFPVSLRKYDIILGNPFVAANYGYPTSTRLILAGTSLNAKTFEQLFLHKLEFVEVTHCGDVFTLFPARLRQGLKNLKEVIVDSCKSLEEVFELGEADEGSSEEKELPLLSSLTELKLRWLPELKCIWPTGHISLQNLAHLELFSLHKLTFIFTPPLARSLSKLEGLYIRNCGELKHIIREEEGEREIIPESPCFPELKNINIYFCDKLEYVLPVSLSHDRDGIIKFPQLRELSLKLGSNYSFLGPRNFDAQLPLQELTIKGHEEVGNWLAQLQQNGFLQRLEYVHVDDCADVRTPFPAKLLRALKNLRMVNIYGCKSLEEVFELGEADEGSSEEKELPLLSSLTELQLQRLPELKCIWKGPTRNVSLQSLVHLNVWCLNKLTFIFTSSLAQSLPKLERLSMSDCGELKHIIREEDGEREIIPESPGFPKLKTISISDCGKLEYVLPVSVSPSLLNLNEMRIFKAHNLKQIFYSGEGDALTRDGIIKFPKLRELYLSNCSFFGPKNFTAQLPSLQILEIDGHKELGNLFAQLQGLTNLETLRLRSLLVPDIRCLWKGLVLSKLTTLNVVECKRLTHVFTRSMIVSLVPLKVLKILSCEELEQIIAKDDDENDQILLGDHLQSLCFPNLCEIEIRECNKLKSLFPLAMASGLPNLQILRVTKASQLLGVFGQDDHASPVNVEKEMVLPNLKKLSLEQLSSIVCFSFGCCDFLFPRLEKLKVYECSKLTIKFAITPNGGSMSAQSEVSQPAEDSSTNREWTS

Target ORF information:

RefSeq Version	XM_024585987.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa probable disease resistance protein At5g63020 (LOC7459153), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024585987.1

ORF Insert Sequence:

ATGCGACAAT TCACGTTAAG CAAGGCACTG GCCAAGAAGT CGGAGACTTT CACAAAACTT 
GGAGAAACAA AGTTTACAAA AGTGGCCGAC GAACGTCCTC CTCAGCGCAT AGAATTTCTA 
ACATCAAAGG AATTCACGCC CTCAAAATCG TCAGAAGAAG CTTTGGAACA GATTATGGAA 
GCTCTCAAAG ATGACAACGT CAATATGATC GGACTGTACG GCATGGGAGG GGTGGGTAAA 
ACCACCTTGG TGAAAGAAGT AGGCAGGAGA GCAAAAGAGT TGCAGCTTTT TCCTGAAGTT 
TTGATGGCTA CGGTGTCCCA GAATCAAAAT GTTACAGACA TCCAGGATCG AATGGCAGAT 
AAGTTATGTC TGGATATTAA AGAAAAGAGT AAAGAAGGGA GAGCAGATCG ATTATGGCAG 
AGACTGAAGC AAGTGGAGAA GATGCTTATA ATCCTGGATG ATGTTTGGAA ACATATTGAC 
TTGAAAGAGA TAGGGATCCC ATTTGGTGAT GATCACAGGG GTTGTAAAAT TCTTCTAACA 
ACACGTCGTC GAGACATATG TTCTTATATG GTGTGCCAGA AAAATGTGTT TCTACGTCTC 
TTTTCTGAAA AAGAAGCATG GGATTTATTC AGAATCAATG CAGGTTTAGA TGATGGGGAC 
TCTACCTTGA ACAGAGTGGC AAGGGATGTT GCGAGAGAAT GCCACGGCTT GCCTATAGCA 
CTTGTGACAA TGGGAAGGGC TCTAAGAGAT GAATCTGCAG TTAAGTGGAA AAGAGTGTCT 
AAACAGCTCA AAAACTCTCA ATTTCCGGAC ATGGAACAAA TTGAAGAAAA AAATGCATAT 
GCATGTCTTA AGTTGAGCTA CGATTATTTG AAGTCCAAGG AAACCAAGTT ATGTTTCTTG 
CTATGCTGTT TATTTCCAGA AGATTACAAC ATTCCAGTCG AGGACTTGAC GAGATATGCA 
GTTGGCTATG GGTTACATCA AGATGGGGAG CCCATTGAAG ATGCAAGGGA ACAAGTTCAT 
GTGGCAATCC AAGACCTCAA ATCTTGCTGT TTGTTGTTGG GCACTGAAAC TGAAGAACAT 
GTGAGAATGC ATGACTTGGT TCGTGATGTT GCTATTCAGA TAGCATCATC AAAAGAATAT 
GGATTCATGG TATTGGAGAA GTGGCCATTG AGTATTGAAA GCTTTGAAGC TTGTACAACA 
ATTTCGTTAA TGGGCAATAA ACTAACCGAA CTTCCTGAAG GATTGGAATG TCCACAGCTC 
AAAGTTCTAT TGTTAGAATT GGATTATGGT TTGTATGTTC CTCAGAGGTT CTTTGAAGGG 
ATGAAAGAAA TTGAAGTTTT GTCTCTGGAG GGAGGGTGTT TGTCATTGCA ATCACTTGAA 
CTCTCAACGA AACTTCAATC GTTGGTGTTG ATCAGGTGTG AATGCAAGGA CCTCATTTGG 
TTGAGAAAGC TGCAGAGACT TAAGATTCTT GTTTTTATAC GGTGCTTATC CATTGAAGAA 
TTACCTGATG AAATAGGAGA GCTCAAGGAG TTAAGGTTGT TGGATGTGAC AGGTTGTCAA 
AGGCTAAGAA GGATCCCTGT GAATTTGATT GGAAGGTTGA AGAAGTTAGA AGAGCTGTTG 
ATCGGGCATC GCAGCTTTGA TGGATGGGAT GTTGTTGGAT GTGACAGCAC CGGAGGAATG 
AATGCAAGCC TGACAGAACT AAATTCGTTG TCTCAGTTAG CCGTATTATC ATTGAGGATC 
CCGAAGGTTG AATGCACTCC CAGAGATTTT GTTTTTCCAG TTAGCTTGCG CAAATATGAT 
ATAATATTAG GGAATCCGTT TGTGGCAGCC AATTATGGAT ACCCAACCTC GACAAGATTA 
ATTTTGGCTG GTACATCCTT AAATGCGAAG ACATTTGAGC AATTGTTTTT ACATAAATTA 
GAATTTGTAG AAGTGACGCA TTGTGGGGAT GTTTTCACTC TGTTTCCAGC AAGATTGCGG 
CAAGGTTTAA AAAATCTGAA GGAGGTGATT GTTGACAGCT GTAAATCATT GGAAGAGGTG 
TTTGAATTGG GTGAGGCTGA TGAAGGAAGC AGTGAGGAGA AGGAGCTGCC GCTGCTGTCA 
TCTTTAACAG AGTTAAAGCT GCGATGGTTA CCTGAGCTCA AATGCATATG GCCCACCGGA 
CATATCAGCC TCCAAAATCT TGCTCATCTG GAGTTGTTTT CTCTCCACAA ACTGACATTT 
ATCTTCACAC CGCCCCTCGC TCGAAGTTTG TCAAAGCTAG AAGGCCTTTA CATAAGAAAT 
TGCGGTGAAT TGAAGCATAT TATCAGAGAA GAGGAAGGTG AAAGGGAAAT AATTCCAGAG 
TCTCCTTGCT TCCCAGAATT AAAAAATATC AACATATATT TCTGTGATAA ACTGGAATAT 
GTCTTGCCAG TCTCTTTATC TCATGACAGA GATGGCATCA TCAAGTTCCC TCAACTAAGA 
GAATTGTCTC TTAAGCTCGG ATCAAATTAC AGCTTTTTAG GTCCAAGGAA TTTTGATGCT 
CAATTGCCTT TGCAAGAATT AACCATTAAA GGCCACGAAG AAGTGGGTAA TTGGTTGGCA 
CAGCTACAAC AAAACGGCTT CTTACAAAGA TTAGAATATG TACACGTGGA CGATTGTGCG 
GATGTTCGCA CTCCGTTTCC AGCAAAATTG CTGCGAGCTT TGAAAAATCT AAGGATGGTG 
AACATATACG GCTGCAAATC ATTGGAAGAG GTATTTGAAT TAGGTGAGGC TGATGAAGGA 
AGCAGTGAGG AGAAGGAGCT GCCGCTGCTG TCATCTTTAA CAGAGTTACA GCTGCAAAGG 
TTACCTGAGC TCAAATGCAT ATGGAAGGGG CCCACCAGAA ATGTCAGCCT CCAAAGTCTT 
GTTCATCTGA ATGTTTGGTG TCTCAACAAA CTGACATTTA TCTTCACATC ATCCCTCGCG 
CAGAGTCTGC CAAAGCTAGA AAGACTTTCC ATGAGTGATT GCGGTGAATT GAAGCATATT 
ATCAGAGAAG AGGATGGTGA AAGGGAAATA ATTCCAGAGT CTCCTGGCTT CCCAAAATTA 
AAAACTATCA GCATATCTGA CTGTGGTAAA CTGGAATATG TCCTCCCTGT CTCTGTGTCT 
CCAAGCCTTC TGAACCTGAA CGAGATGCGG ATTTTTAAAG CTCATAATTT AAAGCAAATA 
TTTTACAGTG GAGAAGGAGA TGCACTCACC AGAGATGGCA TCATCAAGTT CCCTAAGCTA 
AGAGAATTGT ATCTTTCAAA TTGCAGCTTC TTTGGTCCAA AGAATTTTAC TGCCCAATTG 
CCTTCCTTGC AAATTCTAGA AATTGATGGC CACAAAGAAT TGGGAAATTT GTTTGCACAG 
CTCCAAGGGT TGACAAATTT GGAAACATTA CGCTTGCGCT CCCTGCTGGT GCCTGATATT 
AGGTGTCTAT GGAAGGGTCT CGTGTTGAGC AAATTGACTA CTTTGAATGT GGTTGAGTGT 
AAAAGACTGA CGCATGTATT CACACGCAGT ATGATTGTCA GTCTAGTTCC ACTGAAAGTT 
CTAAAGATAT TGTCCTGTGA GGAATTGGAG CAAATCATTG CTAAGGATGA TGATGAAAAC 
GACCAGATAT TGTTAGGAGA TCATCTCCAA TCTTTATGCT TTCCCAATTT GTGTGAAATT 
GAGATTAGAG AATGCAACAA GTTGAAGAGT CTCTTCCCAT TAGCCATGGC TTCAGGTCTC 
CCAAATCTCC AAATACTGAG GGTAACAAAA GCCTCTCAAT TATTGGGAGT CTTTGGGCAG 
GATGATCATG CTTCACCTGT CAACGTTGAG AAGGAGATGG TGCTTCCTAA TCTAAAAAAG 
TTGTCTCTAG AACAATTATC AAGTATTGTC TGCTTCAGTT TCGGATGCTG TGATTTCTTA 
TTCCCTCGTT TGGAGAAGTT GAAGGTGTAT GAATGCTCAA AGTTGACCAT AAAATTTGCT 
ATTACACCAA ATGGTGGTTC AATGAGTGCT CAATCAGAGG TATCTCAACC AGCTGAAGAT 
TCAAGCACTA ATAGAGAGTG GACCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7459153 ( XM_024585992.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024441760.1 Populus trichocarpa probable disease resistance protein At5g63020 (LOC7459153), transcript variant X8, mRNA.

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CloneID	OPa422388
Clone ID Related Accession (Same CDS sequence)	XM_024585992.1
Accession Version	XM_024585992.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	disease resistance protein At4g27190 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTTG AAAGTGCTGG TGGATCCATT ATAGCTATGC TAGCAGAACT CATGGTGGAA 
CCAGTAGGAA GGCTGTTCCG TTACATGTTC TGTTTCAACA ATTTTGTTGA AGAATTCAAA 
GAACGAAAGG AGAACCTTGC TTTAGCACTA GATGGTCTGC AAAAAGAAGT CGAAGCTGCT 
GAAAGGAATG CTGAAGAAAT TAAGAAGGAT GTCAAAAAGT GGCTGGAAGA TGCAAAAAAC 
GAAATTGAAG GTGCGAAGCC ATTGGAAAAT GAAATAGGAA AAAATGGCAA ATGCTTTACT 
TGGTGCCCAA ACTGCATGCG ACAATTCACG TTAAGCAAGG CACTGGCCAA GAAGTCGGAG 
ACTTTCACAA AACTTGGAGA AACAAAGTTT ACAAAAGTGG CCGACGAACG TCCTCCTCAG 
CGCATAGAAT TTCTAACATC AAAGGAATTC ACGCCCTCAA AATCGTCAGA AGAAGCTTTG 
GAACAGATTA TGGAAGCTCT CAAAGATGAC AACGTCAATA TGATCGGACT GTACGGCATG 
GGAGGGGTGG GTAAAACCAC CTTGGTGAAA GAAGTAGGCA GGAGAGCAAA AGAGTTGCAG 
CTTTTTCCTG AAGTTTTGAT GGCTACGGTG TCCCAGAATC AAAATGTTAC AGACATCCAG 
GATCGAATGG CAGATAAGTT ATGTCTGGAT ATTAAAGAAA AGAGTAAAGA AGGGAGAGCA 
GATCGATTAT GGCAGAGACT GAAGCAAGTG GAGAAGATGC TTATAATCCT GGATGATGTT 
TGGAAACATA TTGACTTGAA AGAGATAGGG ATCCCATTTG GTGATGATCA CAGGGGTTGT 
AAAATTCTTC TAACAACACG TCGTCGAGAC ATATGTTCTT ATATGGTGTG CCAGAAAAAT 
GTGTTTCTAC GTCTCTTTTC TGAAAAAGAA GCATGGGATT TATTCAGAAT CAATGCAGGT 
TTAGATGATG GGGACTCTAC CTTGAACAGA GTGGCAAGGG ATGTTGCGAG AGAATGCCAC 
GGCTTGCCTA TAGCACTTGT GACAATGGGA AGGGCTCTAA GAGATGAATC TGCAGTTAAG 
TGGAAAAGAG TGTCTAAACA GCTCAAAAAC TCTCAATTTC CGGACATGGA ACAAATTGAA 
GAAAAAAATG CATATGCATG TCTTAAGTTG AGCTACGATT ATTTGAAGTC CAAGGAAACC 
AAGTTATGTT TCTTGCTATG CTGTTTATTT CCAGAAGATT ACAACATTCC AGTCGAGGAC 
TTGACGAGAT ATGCAGTTGG CTATGGGTTA CATCAAGATG GGGAGCCCAT TGAAGATGCA 
AGGGAACAAG TTCATGTGGC AATCCAAGAC CTCAAATCTT GCTGTTTGTT GTTGGGCACT 
GAAACTGAAG AACATGTGAG AATGCATGAC TTGGTTCGTG ATGTTGCTAT TCAGATAGCA 
TCATCAAAAG AATATGGATT CATGGTATTG GAGAAGTGGC CATTGAGTAT TGAAAGCTTT 
GAAGCTTGTA CAACAATTTC GTTAATGGGC AATAAACTAA CCGAACTTCC TGAAGGATTG 
GAATGTCCAC AGCTCAAAGT TCTATTGTTA GAATTGGATT ATGGTTTGTA TGTTCCTCAG 
AGGTTCTTTG AAGGGATGAA AGAAATTGAA GTTTTGTCTC TGGAGGGAGG GTGTTTGTCA 
TTGCAATCAC TTGAACTCTC AACGAAACTT CAATCGTTGG TGTTGATCAG GTGTGAATGC 
AAGGACCTCA TTTGGTTGAG AAAGCTGCAG AGACTTAAGA TTCTTGTTTT TATACGGTGC 
TTATCCATTG AAGAATTACC TGATGAAATA GGAGAGCTCA AGGAGTTAAG GTTGTTGGAT 
GTGACAGGTT GTCAAAGGCT AAGAAGGATC CCTGTGAATT TGATTGGAAG GTTGAAGAAG 
TTAGAAGAGC TGTTGATCGG GCATCGCAGC TTTGATGGAT GGGATGTTGT TGGATGTGAC 
AGCACCGGAG GAATGAATGC AAGCCTGACA GAACTAAATT CGTTGTCTCA GTTAGCCGTA 
TTATCATTGA GGATCCCGAA GGTTGAATGC ACTCCCAGAG ATTTTGTTTT TCCAGTTAGC 
TTGCGCAAAT ATGATATAAT ATTAGGGAAT CCGTTTGTGG CAGCCAATTA TGGATACCCA 
ACCTCGACAA GATTAATTTT GGCTGGTACA TCCTTAAATG CGAAGACATT TGAGCAATTG 
TTTTTACATA AATTAGAATT TGTAGAAGTG ACGCATTGTG GGGATGTTTT CACTCTGTTT 
CCAGCAAGAT TGCGGCAAGG TTTAAAAAAT CTGAAGGAGG TGATTGTTGA CAGCTGTAAA 
TCATTGGAAG AGGTATTTGA ATTAGGTGAG GCTGATGAAG GAAGCAGTGA GGAGAAGGAG 
CTGCCGCTGC TGTCATCTTT AACAGAGTTA CAGCTGCAAA GGTTACCTGA GCTCAAATGC 
ATATGGAAGG GGCCCACCAG AAATGTCAGC CTCCAAAGTC TTGTTCATCT GAATGTTTGG 
TGTCTCAACA AACTGACATT TATCTTCACA TCATCCCTCG CGCAGAGTCT GCCAAAGCTA 
GAAAGACTTT CCATGAGTGA TTGCGGTGAA TTGAAGCATA TTATCAGAGA AGAGGATGGT 
GAAAGGGAAA TAATTCCAGA GTCTCCTGGC TTCCCAAAAT TAAAAACTAT CAGCATATCT 
GACTGTGGTA AACTGGAATA TGTCCTCCCT GTCTCTGTGT CTCCAAGCCT TCTGAACCTG 
AACGAGATGC GGATTTTTAA AGCTCATAAT TTAAAGCAAA TATTTTACAG TGGAGAAGGA 
GATGCACTCA CCAGAGATGG CATCATCAAG TTCCCTAAGC TAAGAGAATT GTATCTTTCA 
AATTGCAGCT TCTTTGGTCC AAAGAATTTT ACTGCCCAAT TGCCTTCCTT GCAAATTCTA 
GAAATTGATG GCCACAAAGA ATTGGGAAAT TTGTTTGCAC AGCTCCAAGG GTTGACAAAT 
TTGGAAACAT TACGCTTGCG CTCCCTGCTG GTGCCTGATA TTAGGTGTCT ATGGAAGGGT 
CTCGTGTTGA GCAAATTGAC TACTTTGAAT GTGGTTGAGT GTAAAAGACT GACGCATGTA 
TTCACACGCA GTATGATTGT CAGTCTAGTT CCACTGAAAG TTCTAAAGAT ATTGTCCTGT 
GAGGAATTGG AGCAAATCAT TGCTAAGGAT GATGATGAAA ACGACCAGAT ATTGTTAGGA 
GATCATCTCC AATCTTTATG CTTTCCCAAT TTGTGTGAAA TTGAGATTAG AGAATGCAAC 
AAGTTGAAGA GTCTCTTCCC ATTAGCCATG GCTTCAGGTC TCCCAAATCT CCAAATACTG 
AGGGTAACAA AAGCCTCTCA ATTATTGGGA GTCTTTGGGC AGGATGATCA TGCTTCACCT 
GTCAACGTTG AGAAGGAGAT GGTGCTTCCT AATCTAAAAA AGTTGTCTCT AGAACAATTA 
TCAAGTATTG TCTGCTTCAG TTTCGGATGC TGTGATTTCT TATTCCCTCG TTTGGAGAAG 
TTGAAGGTGT ATGAATGCTC AAAGTTGACC ATAAAATTTG CTATTACACC AAATGGTGGT 
TCAATGAGTG CTCAATCAGA GGTATCTCAA CCAGCTGAAG ATTCAAGCAC TAATAGAGAG 
TGGACCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024441760.1
CDS	508..4179
Translation	MALESAGGSIIAMLAELMVEPVGRLFRYMFCFNNFVEEFKERKENLALALDGLQKEVEAAERNAEEIKKDVKKWLEDAKNEIEGAKPLENEIGKNGKCFTWCPNCMRQFTLSKALAKKSETFTKLGETKFTKVADERPPQRIEFLTSKEFTPSKSSEEALEQIMEALKDDNVNMIGLYGMGGVGKTTLVKEVGRRAKELQLFPEVLMATVSQNQNVTDIQDRMADKLCLDIKEKSKEGRADRLWQRLKQVEKMLIILDDVWKHIDLKEIGIPFGDDHRGCKILLTTRRRDICSYMVCQKNVFLRLFSEKEAWDLFRINAGLDDGDSTLNRVARDVARECHGLPIALVTMGRALRDESAVKWKRVSKQLKNSQFPDMEQIEEKNAYACLKLSYDYLKSKETKLCFLLCCLFPEDYNIPVEDLTRYAVGYGLHQDGEPIEDAREQVHVAIQDLKSCCLLLGTETEEHVRMHDLVRDVAIQIASSKEYGFMVLEKWPLSIESFEACTTISLMGNKLTELPEGLECPQLKVLLLELDYGLYVPQRFFEGMKEIEVLSLEGGCLSLQSLELSTKLQSLVLIRCECKDLIWLRKLQRLKILVFIRCLSIEELPDEIGELKELRLLDVTGCQRLRRIPVNLIGRLKKLEELLIGHRSFDGWDVVGCDSTGGMNASLTELNSLSQLAVLSLRIPKVECTPRDFVFPVSLRKYDIILGNPFVAANYGYPTSTRLILAGTSLNAKTFEQLFLHKLEFVEVTHCGDVFTLFPARLRQGLKNLKEVIVDSCKSLEEVFELGEADEGSSEEKELPLLSSLTELQLQRLPELKCIWKGPTRNVSLQSLVHLNVWCLNKLTFIFTSSLAQSLPKLERLSMSDCGELKHIIREEDGEREIIPESPGFPKLKTISISDCGKLEYVLPVSVSPSLLNLNEMRIFKAHNLKQIFYSGEGDALTRDGIIKFPKLRELYLSNCSFFGPKNFTAQLPSLQILEIDGHKELGNLFAQLQGLTNLETLRLRSLLVPDIRCLWKGLVLSKLTTLNVVECKRLTHVFTRSMIVSLVPLKVLKILSCEELEQIIAKDDDENDQILLGDHLQSLCFPNLCEIEIRECNKLKSLFPLAMASGLPNLQILRVTKASQLLGVFGQDDHASPVNVEKEMVLPNLKKLSLEQLSSIVCFSFGCCDFLFPRLEKLKVYECSKLTIKFAITPNGGSMSAQSEVSQPAEDSSTNREWTS

Target ORF information:

RefSeq Version	XM_024585992.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa probable disease resistance protein At5g63020 (LOC7459153), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024585992.1

ORF Insert Sequence:

ATGGCTCTTG AAAGTGCTGG TGGATCCATT ATAGCTATGC TAGCAGAACT CATGGTGGAA 
CCAGTAGGAA GGCTGTTCCG TTACATGTTC TGTTTCAACA ATTTTGTTGA AGAATTCAAA 
GAACGAAAGG AGAACCTTGC TTTAGCACTA GATGGTCTGC AAAAAGAAGT CGAAGCTGCT 
GAAAGGAATG CTGAAGAAAT TAAGAAGGAT GTCAAAAAGT GGCTGGAAGA TGCAAAAAAC 
GAAATTGAAG GTGCGAAGCC ATTGGAAAAT GAAATAGGAA AAAATGGCAA ATGCTTTACT 
TGGTGCCCAA ACTGCATGCG ACAATTCACG TTAAGCAAGG CACTGGCCAA GAAGTCGGAG 
ACTTTCACAA AACTTGGAGA AACAAAGTTT ACAAAAGTGG CCGACGAACG TCCTCCTCAG 
CGCATAGAAT TTCTAACATC AAAGGAATTC ACGCCCTCAA AATCGTCAGA AGAAGCTTTG 
GAACAGATTA TGGAAGCTCT CAAAGATGAC AACGTCAATA TGATCGGACT GTACGGCATG 
GGAGGGGTGG GTAAAACCAC CTTGGTGAAA GAAGTAGGCA GGAGAGCAAA AGAGTTGCAG 
CTTTTTCCTG AAGTTTTGAT GGCTACGGTG TCCCAGAATC AAAATGTTAC AGACATCCAG 
GATCGAATGG CAGATAAGTT ATGTCTGGAT ATTAAAGAAA AGAGTAAAGA AGGGAGAGCA 
GATCGATTAT GGCAGAGACT GAAGCAAGTG GAGAAGATGC TTATAATCCT GGATGATGTT 
TGGAAACATA TTGACTTGAA AGAGATAGGG ATCCCATTTG GTGATGATCA CAGGGGTTGT 
AAAATTCTTC TAACAACACG TCGTCGAGAC ATATGTTCTT ATATGGTGTG CCAGAAAAAT 
GTGTTTCTAC GTCTCTTTTC TGAAAAAGAA GCATGGGATT TATTCAGAAT CAATGCAGGT 
TTAGATGATG GGGACTCTAC CTTGAACAGA GTGGCAAGGG ATGTTGCGAG AGAATGCCAC 
GGCTTGCCTA TAGCACTTGT GACAATGGGA AGGGCTCTAA GAGATGAATC TGCAGTTAAG 
TGGAAAAGAG TGTCTAAACA GCTCAAAAAC TCTCAATTTC CGGACATGGA ACAAATTGAA 
GAAAAAAATG CATATGCATG TCTTAAGTTG AGCTACGATT ATTTGAAGTC CAAGGAAACC 
AAGTTATGTT TCTTGCTATG CTGTTTATTT CCAGAAGATT ACAACATTCC AGTCGAGGAC 
TTGACGAGAT ATGCAGTTGG CTATGGGTTA CATCAAGATG GGGAGCCCAT TGAAGATGCA 
AGGGAACAAG TTCATGTGGC AATCCAAGAC CTCAAATCTT GCTGTTTGTT GTTGGGCACT 
GAAACTGAAG AACATGTGAG AATGCATGAC TTGGTTCGTG ATGTTGCTAT TCAGATAGCA 
TCATCAAAAG AATATGGATT CATGGTATTG GAGAAGTGGC CATTGAGTAT TGAAAGCTTT 
GAAGCTTGTA CAACAATTTC GTTAATGGGC AATAAACTAA CCGAACTTCC TGAAGGATTG 
GAATGTCCAC AGCTCAAAGT TCTATTGTTA GAATTGGATT ATGGTTTGTA TGTTCCTCAG 
AGGTTCTTTG AAGGGATGAA AGAAATTGAA GTTTTGTCTC TGGAGGGAGG GTGTTTGTCA 
TTGCAATCAC TTGAACTCTC AACGAAACTT CAATCGTTGG TGTTGATCAG GTGTGAATGC 
AAGGACCTCA TTTGGTTGAG AAAGCTGCAG AGACTTAAGA TTCTTGTTTT TATACGGTGC 
TTATCCATTG AAGAATTACC TGATGAAATA GGAGAGCTCA AGGAGTTAAG GTTGTTGGAT 
GTGACAGGTT GTCAAAGGCT AAGAAGGATC CCTGTGAATT TGATTGGAAG GTTGAAGAAG 
TTAGAAGAGC TGTTGATCGG GCATCGCAGC TTTGATGGAT GGGATGTTGT TGGATGTGAC 
AGCACCGGAG GAATGAATGC AAGCCTGACA GAACTAAATT CGTTGTCTCA GTTAGCCGTA 
TTATCATTGA GGATCCCGAA GGTTGAATGC ACTCCCAGAG ATTTTGTTTT TCCAGTTAGC 
TTGCGCAAAT ATGATATAAT ATTAGGGAAT CCGTTTGTGG CAGCCAATTA TGGATACCCA 
ACCTCGACAA GATTAATTTT GGCTGGTACA TCCTTAAATG CGAAGACATT TGAGCAATTG 
TTTTTACATA AATTAGAATT TGTAGAAGTG ACGCATTGTG GGGATGTTTT CACTCTGTTT 
CCAGCAAGAT TGCGGCAAGG TTTAAAAAAT CTGAAGGAGG TGATTGTTGA CAGCTGTAAA 
TCATTGGAAG AGGTATTTGA ATTAGGTGAG GCTGATGAAG GAAGCAGTGA GGAGAAGGAG 
CTGCCGCTGC TGTCATCTTT AACAGAGTTA CAGCTGCAAA GGTTACCTGA GCTCAAATGC 
ATATGGAAGG GGCCCACCAG AAATGTCAGC CTCCAAAGTC TTGTTCATCT GAATGTTTGG 
TGTCTCAACA AACTGACATT TATCTTCACA TCATCCCTCG CGCAGAGTCT GCCAAAGCTA 
GAAAGACTTT CCATGAGTGA TTGCGGTGAA TTGAAGCATA TTATCAGAGA AGAGGATGGT 
GAAAGGGAAA TAATTCCAGA GTCTCCTGGC TTCCCAAAAT TAAAAACTAT CAGCATATCT 
GACTGTGGTA AACTGGAATA TGTCCTCCCT GTCTCTGTGT CTCCAAGCCT TCTGAACCTG 
AACGAGATGC GGATTTTTAA AGCTCATAAT TTAAAGCAAA TATTTTACAG TGGAGAAGGA 
GATGCACTCA CCAGAGATGG CATCATCAAG TTCCCTAAGC TAAGAGAATT GTATCTTTCA 
AATTGCAGCT TCTTTGGTCC AAAGAATTTT ACTGCCCAAT TGCCTTCCTT GCAAATTCTA 
GAAATTGATG GCCACAAAGA ATTGGGAAAT TTGTTTGCAC AGCTCCAAGG GTTGACAAAT 
TTGGAAACAT TACGCTTGCG CTCCCTGCTG GTGCCTGATA TTAGGTGTCT ATGGAAGGGT 
CTCGTGTTGA GCAAATTGAC TACTTTGAAT GTGGTTGAGT GTAAAAGACT GACGCATGTA 
TTCACACGCA GTATGATTGT CAGTCTAGTT CCACTGAAAG TTCTAAAGAT ATTGTCCTGT 
GAGGAATTGG AGCAAATCAT TGCTAAGGAT GATGATGAAA ACGACCAGAT ATTGTTAGGA 
GATCATCTCC AATCTTTATG CTTTCCCAAT TTGTGTGAAA TTGAGATTAG AGAATGCAAC 
AAGTTGAAGA GTCTCTTCCC ATTAGCCATG GCTTCAGGTC TCCCAAATCT CCAAATACTG 
AGGGTAACAA AAGCCTCTCA ATTATTGGGA GTCTTTGGGC AGGATGATCA TGCTTCACCT 
GTCAACGTTG AGAAGGAGAT GGTGCTTCCT AATCTAAAAA AGTTGTCTCT AGAACAATTA 
TCAAGTATTG TCTGCTTCAG TTTCGGATGC TGTGATTTCT TATTCCCTCG TTTGGAGAAG 
TTGAAGGTGT ATGAATGCTC AAAGTTGACC ATAAAATTTG CTATTACACC AAATGGTGGT 
TCAATGAGTG CTCAATCAGA GGTATCTCAA CCAGCTGAAG ATTCAAGCAC TAATAGAGAG 
TGGACCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)
Tuskan GA,Difazio S,Jansson S,Bohlmann J,Grigoriev I,Hellsten U,Putnam N,Ralph S,Rombauts S,Salamov A,Schein J,Sterck L,Aerts A,Bhalerao RR,Bhalerao RP,Blaudez D,Boerjan W,Brun A,Brunner A,Busov V,Campbell M,Carlson J,Chalot M,Chapman J,Chen GL,Cooper D,Coutinho PM,Couturier J,Covert S,Cronk Q,Cunningham R,Davis J,Degroeve S,Déjardin A,Depamphilis C,Detter J,Dirks B,Dubchak I,Duplessis S,Ehlting J,Ellis B,Gendler K,Goodstein D,Gribskov M,Grimwood J,Groover A,Gunter L,Hamberger B,Heinze B,Helariutta Y,Henrissat B,Holligan D,Holt R,Huang W,Islam-Faridi N,Jones S,Jones-Rhoades M,Jorgensen R,Joshi C,Kangasjärvi J,Karlsson J,Kelleher C,Kirkpatrick R,Kirst M,Kohler A,Kalluri U,Larimer F,Leebens-Mack J,Leplé JC,Locascio P,Lou Y,Lucas S,Martin F,Montanini B,Napoli C,Nelson DR,Nelson C,Nieminen K,Nilsson O,Pereda V,Peter G,Philippe R,Pilate G,Poliakov A,Razumovskaya J,Richardson P,Rinaldi C,Ritland K,Rouzé P,Ryaboy D,Schmutz J,Schrader J,Segerman B,Shin H,Siddiqui A,Sterky F,Terry A,Tsai CJ,Uberbacher E,Unneberg P,Vahala J,Wall K,Wessler S,Yang G,Yin T,Douglas C,Marra M,Sandberg G,Van de Peer Y,Rokhsar D

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LOC7459153 cDNA ORF clone, Populus trichocarpa(black cottonwood)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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