LOC18107958 gene cDNA ORF clone, Populus trichocarpa(black cottonwood)

mRNA	Protein	Name
XM_024591920.1	XP_024447688.1	probable disease resistance protein At4g27220 isoform X1
XM_024591911.1	XP_024447679.1	probable disease resistance protein At4g27220 isoform X1
XM_024591912.1	XP_024447680.1	probable disease resistance protein At4g27220 isoform X1
XM_024591917.1	XP_024447685.1	probable disease resistance protein At4g27220 isoform X1
XM_024591913.1	XP_024447681.1	probable disease resistance protein At4g27220 isoform X1
XM_024591916.1	XP_024447684.1	probable disease resistance protein At4g27220 isoform X1
XM_024591922.1	XP_024447690.1	probable disease resistance protein At4g27220 isoform X2
XM_024591918.1	XP_024447686.1	probable disease resistance protein At4g27220 isoform X1
XM_024591919.1	XP_024447687.1	probable disease resistance protein At4g27220 isoform X1
XM_006370801.1	XP_006370863.1	hypothetical protein
XM_024591910.1	XP_024447678.1	probable disease resistance protein At4g27220 isoform X1
XM_024591914.1	XP_024447682.1	probable disease resistance protein At4g27220 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPa37289	XM_006370801.1 Latest version!	Populus trichocarpa hypothetical protein (POPTR_0019s01100g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPa418670	XM_024591916.1 Latest version!	Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPa418670	XM_024591913.1 Latest version!	Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPa418670	XM_024591910.1 Latest version!	Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPa418670	XM_024591920.1 Latest version!	Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPa418670	XM_024591914.1 Latest version!	Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPa418670	XM_024591918.1 Latest version!	Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPa418670	XM_024591912.1 Latest version!	Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPa418670	XM_024591911.1 Latest version!	Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPa418670	XM_024591917.1 Latest version!	Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPa418670	XM_024591919.1 Latest version!	Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPa418671	XM_024591922.1 Latest version!	Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

LOC18107958 ( XM_006370801.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_006370863.1 Populus trichocarpa hypothetical protein (POPTR_0019s01100g) mRNA, complete cds.

Price & Availability↑

CloneID	OPa37289
Clone ID Related Accession (Same CDS sequence)	XM_006370801.1
Accession Version	XM_006370801.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1388332800000
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008485). COMPLETENESS: incomplete on the 3' end.

ATGGTTCGAT CAAATGATCC ATTTTGGAAT GATGTTGAAG ATATGAATAA TGGTGGCATG 
AAGTGTAAAT TTTGTGGGCA TTTATTTTCC GAGAATACTT CCATTTCGAG GATCAAATGG 
CATTTGTCAG GAGTGAAAGG GTGGGGTGTT AAAATATGTG AGAAAGTTCC TGAAGAAGTT 
CAAAATGTAG TCCGTGCAGC AATGGATGGC CCTCCAGAAA AAAAACTTAA AACCGTAGCA 
GGCTCAAGCA ATAACAAGGT CACTATTGCA GTTTTAGCTT CTGCCCAAGA ACAAAACAAT 
GATGAAGTGA TGGCACAAGA AGGAGATGCT CTTTTCGTTG GAGAGCGCGA ACATTGGCTG 
AGTAGCTTCA CTGATGAAGA CATTGAGCTT CTGAGAGGTA TCTTTCATGA GAGAACATCA 
ATTAATCAAG CTGATGAGCC TCGAGGAGAT CCATCCCAGC CAACAGATCC ATTGTGTCTT 
GACCATGGAA GATATTATGA TCGACCCCGT GCTCCATTAG TAAACAATGA TGTAATTCTG 
AATCTTGTGG AGGACATGCT GGTGGGTCAA GCATTCGAAG ACCACAAGAA GACTATCTGG 
TCTTGGTTAA TGCATGATAA AGTTTCAAGA ATTAGCATCT ATGGGATGGG CGGAGTGGGT 
AAGACGACGT TGGTGACACA CATATATAAT CAGCTTCTAG AAAGACCAGA CACTTTCAAT 
CATGTTTTCT GGATTACTGT TTCACAAGAT ACCAGCATAA ACAGTTTGCA GAAAAGTTTC 
GCAAGATGTA TTGGTTTAGA TCTTTCTAAT GAAGATGAGG AGCTGCACAG AGCGGTCATA 
TTGAGAAAAG AATTAATGAA GAAAAAGAAA TGGGTTTTGA TTTTAGATGA TCTGTGGAAG 
GCTTTTGACT TACAAAAGCT GGGAATTCCT GACCAAGTTA AGGGATGCAA GTTGATTATT 
ACAACTCGAT CAGGAAGAAT TTGTCAACAG ATGAAAACCC AACACACAAT CAAAGTGCAG 
CCTATTTTGA AGGAAGAAGC TTGGATTTTG TTTATTGAGA GGCTTGGAAA TGACATAGCA 
CTTTCTCCAG AAGTGGAACG AATTGCAGTA GATGTTGCAA GGGAATGTGC CGGTTTGCCA 
TTGGGAATTA TTACAATGGC AGGAAGCATG AGGGGAGTGG ATGACCTACA TGAGTGGAGA 
AATACATTGA AGAAATTGAA AGAATCAAAA TTTAGAGACA TGGAAGATGA GGTATTCCAG 
TTGTTGAGGT TGAGTTATGA TCGGCTAGAA AATGATTTAG CACTACAACA ATGTCTCTTG 
TATTGTGCAT TATATCCTGA AGATCATAAG ATTGAAAGGG AGGAGTTGAT ATGTTATTTG 
ATTGATGAGG GAATAGTTGA AGGAACGAGT AGCAGACAAG CAGCATTTGA CGAGGGCCAC 
ACGATGCTTA ATAAACTTGA AAATGTTTGT TTACTGGAAA GTTGTTTTGA TCGTTATCAT 
CATAGATGTG TCAAGATGCA CGACTTGATT AGGGACATGG CCCTCCAAAT ACTCCAAATG 
AACTCTCCAG TTATGGTCAG GGAATATGAG GAAGAAATAC CAGATGTGGA TATGTGGAAA 
GAAGATCTAG TGAGAGTCTC TTTGAAGGAT TGTTATTTCA AGGAAATTCC ATCCAGCCAT 
TCACCAAGGT GTCCCAATCT GTCAACTCTG TTGTTACGCA ATAATGAAGG GTTGGAATTT 
ATTGCAGCTT CTTTTTTTGA GCAATTGCAC GGACTCAAGG TTCTTAATCT GTCTCATACA 
AATATCATAA ATTTGCCTGA TTCTGTTTCT GAATTGGTGA GTCTTACTGC ATTATTGCTC 
AAAGGGTGTG TGAACTTAAG GCTTGTACCA TCATTAAAAA AGCTCAAGGC ACTGAAGAGG 
TTAGACCTCT CTCGTACTGC ACTTGAAAAG ATGCCTCAAG GCATGGAATG TGTATCCAAC 
CTGAGATATC TTAGGATGAA TGGATTTGGG GAAAAGGAGT TTCCTAATGG GATATTACCT 
AAACTCTCTC ACCTACAAGT CTTTGTATTA GAGGATGCGT TTCTTACTTG TCCGATAATA 
GTCAAAGGAA AGGAAGTAGG ATGCTTGAAG GAGTTGGAAA CTGTGGTATG CCATTTTGAA 
GGTCAATCTG ACTTTGTGGA GTATCTTAAT TCTCGGGACA AGACCCAATC ACTAAGCACA 
TACCAAATCT TTGTAGGACT GCTGGATGAA GATGATTATA GTAGAATGAT GGATGATTAT 
CACACAAGTA AAACAATTGC CTTGTGTAAT TTGAGTATCA ATAGGGATGG AGATTTTCAG 
GTCATGTTCC CAAATGACAT TCAAGAACTG GTTATTTATA AATGTTCATG TGATGTTTCC 
TCTCTAATAA AGTATGCAAC TGATCTGGAG GTTATCAAGA TATGGGATAG CAATAGCATC 
GAGAGCTTGG TTTCATCTTC TTGGTTCTGC TCTGCTCCCT TACCATTGCC ATCTTATAAT 
GGTATATTTT CTGGTCTTAA AGAGTTTAAT TGCTATGGAT GTAGAAGTAT GAAGAAGTTG 
TTCCCTATTC TCTTGCTACC ATACCTCGTA AACTTGAAAG TGATTAGAGT TTTTTATTGT 
GAGAAGATGG AGGAGATAGT AGGGACAAGA TCAGAAGAAG AAGCGGTTAT GGTTGAAGAA 
AGCAGCAGTA GCAGCACCGA ATTCAAACTC CCAAAGTTAA GAAATCTGGA ATTGAGGGGA 
TTACCAGAAC TGAAAAGCAT TTGTAGTGCA AAACTGGTCT GTGATTCTCT CCAACATATT 
TGGATAGCAA ATTGTCAGAA GTTGAAGAGA ATGCCAATTT GTCTTCCGTT GCTTGAAAAT 
GGCCAGCCAT CTGCTCTCCC TTCTCTTCAA AATATCTTTA TATATCCAAG AGAATGGTGG 
GAGTCTGTAG TGGAGTGGGA TCATCCTAAT GCTAAGAACG TCCTTCGTCC CTTCGTAAAG 
TTTTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006370863.1
CDS	9..3017
Translation	MVRSNDPFWNDVEDMNNGGMKCKFCGHLFSENTSISRIKWHLSGVKGWGVKICEKVPEEVQNVVRAAMDGPPEKKLKTVAGSSNNKVTIAVLASAQEQNNDEVMAQEGDALFVGEREHWLSSFTDEDIELLRGIFHERTSINQADEPRGDPSQPTDPLCLDHGRYYDRPRAPLVNNDVILNLVEDMLVGQAFEDHKKTIWSWLMHDKVSRISIYGMGGVGKTTLVTHIYNQLLERPDTFNHVFWITVSQDTSINSLQKSFARCIGLDLSNEDEELHRAVILRKELMKKKKWVLILDDLWKAFDLQKLGIPDQVKGCKLIITTRSGRICQQMKTQHTIKVQPILKEEAWILFIERLGNDIALSPEVERIAVDVARECAGLPLGIITMAGSMRGVDDLHEWRNTLKKLKESKFRDMEDEVFQLLRLSYDRLENDLALQQCLLYCALYPEDHKIEREELICYLIDEGIVEGTSSRQAAFDEGHTMLNKLENVCLLESCFDRYHHRCVKMHDLIRDMALQILQMNSPVMVREYEEEIPDVDMWKEDLVRVSLKDCYFKEIPSSHSPRCPNLSTLLLRNNEGLEFIAASFFEQLHGLKVLNLSHTNIINLPDSVSELVSLTALLLKGCVNLRLVPSLKKLKALKRLDLSRTALEKMPQGMECVSNLRYLRMNGFGEKEFPNGILPKLSHLQVFVLEDAFLTCPIIVKGKEVGCLKELETVVCHFEGQSDFVEYLNSRDKTQSLSTYQIFVGLLDEDDYSRMMDDYHTSKTIALCNLSINRDGDFQVMFPNDIQELVIYKCSCDVSSLIKYATDLEVIKIWDSNSIESLVSSSWFCSAPLPLPSYNGIFSGLKEFNCYGCRSMKKLFPILLLPYLVNLKVIRVFYCEKMEEIVGTRSEEEAVMVEESSSSSTEFKLPKLRNLELRGLPELKSICSAKLVCDSLQHIWIANCQKLKRMPICLPLLENGQPSALPSLQNIFIYPREWWESVVEWDHPNAKNVLRPFVKFL

Target ORF information:

RefSeq Version	XM_006370801.1
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa hypothetical protein (POPTR_0019s01100g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006370801.1

ORF Insert Sequence:

ATGGTTCGAT CAAATGATCC ATTTTGGAAT GATGTTGAAG ATATGAATAA TGGTGGCATG 
AAGTGTAAAT TTTGTGGGCA TTTATTTTCC GAGAATACTT CCATTTCGAG GATCAAATGG 
CATTTGTCAG GAGTGAAAGG GTGGGGTGTT AAAATATGTG AGAAAGTTCC TGAAGAAGTT 
CAAAATGTAG TCCGTGCAGC AATGGATGGC CCTCCAGAAA AAAAACTTAA AACCGTAGCA 
GGCTCAAGCA ATAACAAGGT CACTATTGCA GTTTTAGCTT CTGCCCAAGA ACAAAACAAT 
GATGAAGTGA TGGCACAAGA AGGAGATGCT CTTTTCGTTG GAGAGCGCGA ACATTGGCTG 
AGTAGCTTCA CTGATGAAGA CATTGAGCTT CTGAGAGGTA TCTTTCATGA GAGAACATCA 
ATTAATCAAG CTGATGAGCC TCGAGGAGAT CCATCCCAGC CAACAGATCC ATTGTGTCTT 
GACCATGGAA GATATTATGA TCGACCCCGT GCTCCATTAG TAAACAATGA TGTAATTCTG 
AATCTTGTGG AGGACATGCT GGTGGGTCAA GCATTCGAAG ACCACAAGAA GACTATCTGG 
TCTTGGTTAA TGCATGATAA AGTTTCAAGA ATTAGCATCT ATGGGATGGG CGGAGTGGGT 
AAGACGACGT TGGTGACACA CATATATAAT CAGCTTCTAG AAAGACCAGA CACTTTCAAT 
CATGTTTTCT GGATTACTGT TTCACAAGAT ACCAGCATAA ACAGTTTGCA GAAAAGTTTC 
GCAAGATGTA TTGGTTTAGA TCTTTCTAAT GAAGATGAGG AGCTGCACAG AGCGGTCATA 
TTGAGAAAAG AATTAATGAA GAAAAAGAAA TGGGTTTTGA TTTTAGATGA TCTGTGGAAG 
GCTTTTGACT TACAAAAGCT GGGAATTCCT GACCAAGTTA AGGGATGCAA GTTGATTATT 
ACAACTCGAT CAGGAAGAAT TTGTCAACAG ATGAAAACCC AACACACAAT CAAAGTGCAG 
CCTATTTTGA AGGAAGAAGC TTGGATTTTG TTTATTGAGA GGCTTGGAAA TGACATAGCA 
CTTTCTCCAG AAGTGGAACG AATTGCAGTA GATGTTGCAA GGGAATGTGC CGGTTTGCCA 
TTGGGAATTA TTACAATGGC AGGAAGCATG AGGGGAGTGG ATGACCTACA TGAGTGGAGA 
AATACATTGA AGAAATTGAA AGAATCAAAA TTTAGAGACA TGGAAGATGA GGTATTCCAG 
TTGTTGAGGT TGAGTTATGA TCGGCTAGAA AATGATTTAG CACTACAACA ATGTCTCTTG 
TATTGTGCAT TATATCCTGA AGATCATAAG ATTGAAAGGG AGGAGTTGAT ATGTTATTTG 
ATTGATGAGG GAATAGTTGA AGGAACGAGT AGCAGACAAG CAGCATTTGA CGAGGGCCAC 
ACGATGCTTA ATAAACTTGA AAATGTTTGT TTACTGGAAA GTTGTTTTGA TCGTTATCAT 
CATAGATGTG TCAAGATGCA CGACTTGATT AGGGACATGG CCCTCCAAAT ACTCCAAATG 
AACTCTCCAG TTATGGTCAG GGAATATGAG GAAGAAATAC CAGATGTGGA TATGTGGAAA 
GAAGATCTAG TGAGAGTCTC TTTGAAGGAT TGTTATTTCA AGGAAATTCC ATCCAGCCAT 
TCACCAAGGT GTCCCAATCT GTCAACTCTG TTGTTACGCA ATAATGAAGG GTTGGAATTT 
ATTGCAGCTT CTTTTTTTGA GCAATTGCAC GGACTCAAGG TTCTTAATCT GTCTCATACA 
AATATCATAA ATTTGCCTGA TTCTGTTTCT GAATTGGTGA GTCTTACTGC ATTATTGCTC 
AAAGGGTGTG TGAACTTAAG GCTTGTACCA TCATTAAAAA AGCTCAAGGC ACTGAAGAGG 
TTAGACCTCT CTCGTACTGC ACTTGAAAAG ATGCCTCAAG GCATGGAATG TGTATCCAAC 
CTGAGATATC TTAGGATGAA TGGATTTGGG GAAAAGGAGT TTCCTAATGG GATATTACCT 
AAACTCTCTC ACCTACAAGT CTTTGTATTA GAGGATGCGT TTCTTACTTG TCCGATAATA 
GTCAAAGGAA AGGAAGTAGG ATGCTTGAAG GAGTTGGAAA CTGTGGTATG CCATTTTGAA 
GGTCAATCTG ACTTTGTGGA GTATCTTAAT TCTCGGGACA AGACCCAATC ACTAAGCACA 
TACCAAATCT TTGTAGGACT GCTGGATGAA GATGATTATA GTAGAATGAT GGATGATTAT 
CACACAAGTA AAACAATTGC CTTGTGTAAT TTGAGTATCA ATAGGGATGG AGATTTTCAG 
GTCATGTTCC CAAATGACAT TCAAGAACTG GTTATTTATA AATGTTCATG TGATGTTTCC 
TCTCTAATAA AGTATGCAAC TGATCTGGAG GTTATCAAGA TATGGGATAG CAATAGCATC 
GAGAGCTTGG TTTCATCTTC TTGGTTCTGC TCTGCTCCCT TACCATTGCC ATCTTATAAT 
GGTATATTTT CTGGTCTTAA AGAGTTTAAT TGCTATGGAT GTAGAAGTAT GAAGAAGTTG 
TTCCCTATTC TCTTGCTACC ATACCTCGTA AACTTGAAAG TGATTAGAGT TTTTTATTGT 
GAGAAGATGG AGGAGATAGT AGGGACAAGA TCAGAAGAAG AAGCGGTTAT GGTTGAAGAA 
AGCAGCAGTA GCAGCACCGA ATTCAAACTC CCAAAGTTAA GAAATCTGGA ATTGAGGGGA 
TTACCAGAAC TGAAAAGCAT TTGTAGTGCA AAACTGGTCT GTGATTCTCT CCAACATATT 
TGGATAGCAA ATTGTCAGAA GTTGAAGAGA ATGCCAATTT GTCTTCCGTT GCTTGAAAAT 
GGCCAGCCAT CTGCTCTCCC TTCTCTTCAA AATATCTTTA TATATCCAAG AGAATGGTGG 
GAGTCTGTAG TGGAGTGGGA TCATCCTAAT GCTAAGAACG TCCTTCGTCC CTTCGTAAAG 
TTTTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18107958 ( XM_024591920.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024447688.1 Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OPa418670
Clone ID Related Accession (Same CDS sequence)	XM_024591920.1 , XM_024591911.1 , XM_024591912.1 , XM_024591917.1 , XM_024591913.1 , XM_024591916.1 , XM_024591918.1 , XM_024591919.1 , XM_024591910.1 , XM_024591914.1
Accession Version	XM_024591920.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	probable disease resistance protein At4g27220 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020167869.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCGAC CAAATGATCC TTTTTGGGAA TATGCTGAAA AGATGGATGA TGGTAGCATG 
ACGTGTACGT TTTGTGGGCA TTGTTTTTCC CAAGGTACTT CCGTTACGAG GATCAAATTG 
CATTTGGCAG GAGTGAAACG GCGTGGTGTT AAAATTTGTA AAAATGTTCC AGAAGAAGTT 
CGAGATGCAG CTTGTGATGC AGTTAATAAT AGCCCTCCAG AAAAGAAACT TAAATCTGTT 
GCGGGCTCAA GCAGTAACGA GGTCGCTAAT GCAATTTCAG CTTCAACGCA AGAACAGAAC 
AATGAAGTGA CCCATGTAGA GATGGCACAA CAAGGAGGAC CTTTTTTCAC TGGAGAACTT 
GCATGGGCAA ATGATTTGAT TGGAGAGGCT GAACTTGTGC AGCTGGAGAG AGGTAGTTCT 
CATGAGAGGC CATCAATTAA TCAAACAGAT GAGCCTCGAG GAGATTCATC CCGACCAACA 
GATGATCAAC TCTGTTCTCC ATCAGTAAAC AATGATGCCA ATATGAACGA TGTGCAGAAC 
ATGGTTGCAG TCAGGATAGA ACCAGTACCG GTTCAAGTGT TGGAACAAAG CAATGCAGAA 
GTTGACAGTT TGGCAGGGCA TGCTGGAAGG ATACAAGTGG GAGTTCATGG CATGGAACAA 
GGTGCGGAGG AAGAGAGAAT TTGTCCGAAC TCAGCGGCAA ATGGCATGGA AAACACTGGT 
GATGGATCTA GTCAGCATTC CAGGAGACTC ATGATTGATG CACATGATAA TACAGGGGAA 
GAAGCTACAC AAAGAATAGA TCTAGTGCAA CGATTAGAAG GGGAAACTTG GGGTCAGATG 
AATGCAATCT CTGCTTTTTT AATGGGGGAG GAGGACGTGG AGAACAATCG TGGAAGATTA 
GTGCAGCCTG GAGCAGCTTG CTCTTCTGGA GGGGCTGCAT GCAACACAAA TAAGATTAAA 
GGAGATGCAC TGCCAACGAG AAAAATGGTG GGTCAAGCAT TCGAAGAACA TAAGAAGACT 
ATCTCATCTT TGTTGATGCG CAATGAAGTT TCAAGTATTG GTATCTATGG GATGGGCGGA 
GTGGGAAAGA CCACATTGGG GACACATATA CATAATCAGC TTCTAGAAAG ACCAGAGACT 
CCTGTTTACT GGATTACTGT TTCACATAAT ACCAGCATTC CTAGATTGCA GACAAGTCTC 
GCAGGACGAA TTGGTTTAGA TCTTTCCAAG GTAGATGAGG AGCTGCATAG AGCGGTAGCG 
TTGAAAAAAG AATTAATGAA GAAACAGAAA TGGATTCTGA TTTTAGATGA TTTGTGGAAG 
GCTTTTGACC TGCAAAAGCT GGGAGTTCCT GACCAAGTTG AGGAGGGATG CAAGCTGATT 
CTTACATCTC GATCAGCAAA GGTTTGTCAA CAGATGAAAA CCCAACACAC GATAAAAGTG 
CAGCCTATTT CGGAGAAAGA AGCTTGGACT TTGTTTATTG AGAGACTTGG ACATGACATA 
GCATTTTCTT CAGAAGTGGA AGGAATTGCT TTGAATGTTG TAAGGGAATG TGCTGGTTTG 
CCATTGGGAA TTATTACAAT AGCAGCAAGC ATGAGAGGAG TGGATGAGCC ACATGAGTGG 
AGGAATACAT TGAAGAAATT GAAAGAATCA AAATATAAGG AAATGGAAGA TGAGGTATTC 
CGATTATTGA GGTTTAGTTA TGATCAGCTA AATGATTTAG CATTACAACA ATGTCTCTTG 
TACTGTGCAT TGTATCCTGA AGATCATAGG ATTGAAAGGG AGGAGCTGAT AGGTTATTTG 
ATCGACGAGG AAATAATTGA AGGAATGAGG AGCAGGCAAG CAGCATTTGA CGAGGGCCGC 
ACGATGCTTG ATAAACTTGA AAAAGTCTGT TTATTGGAAA GAGCTTGTTA TGGTGATCAT 
AGTACAACTG TCAAGATGCA TGACTTGATT AGGGACATGG CCCACCAAAT ACTCCAAACA 
AACTCTCCAG TCATGGTTGG GGGATATAAT GATAAATTAC CAGATGTGGA TATGTGGAAA 
GAGAATCTAG TGAGAGTCTC TCTGAAGCAT TGTTATTTCG AGGAAATTCC TTCTAGTCAT 
TCACCAAGAT GTCCCAATCT TTCAACTCTA TTGCTGTGTG ATAATCCTTA TTTGCAATTT 
ATTGCAGATT CTTTTTTCAC ACAATTGCAT GGGCTCAAGG TTCTTGATCT GTCTCGTACA 
GAAATCATAG AATTGCCTGA TTCTGTCTCT GAACTGGTGA GTCTCACTGC ATTATTGCTC 
AAACAATGTG AATATTTAAT ACATGTACCA TCATTAGAAA AGCTCACAGC ACTGAGGAGG 
CTAGATCTCT CTGGTACTTG GGAACTTGAA AAGATACCTC AAGACATGCA ATGTCTATCT 
AACCTGAGGT ATCTGAGGAT GGATGGATGT GGTGTAAAGG AGTTTCCTAC TGGGATATTA 
CCAAAACTCT TTCACCTACA ACTCTTCATG TTAGAGGGGA AGACTAATTA TGACTACGTT 
CAAGTAACAG TTAAGGGAAA GGAAGTAGGA TGCTTGAGGG AGTTGGAAAA TTTGGTATGC 
AATTTTGAAG GTCAATCTGA CTTTGTGGAG TATCTCAATT CTCGGGATAA GACCCGATCA 
CTAAGTACAT ACGATATTTT TGTAGGACCA CTGGATGAAG ATTTTTATAG TGAAATGAAG 
CGTGGTTATG GCAAAAGTAA AACAGTTTGG TTGAGTAGTT TGTGTAACAA TGGAGATGGA 
AATTTTCAAG TAATGTTCCC AAATGACATT CAAAAACTGA TTATTTTTAA TTGTTCATGT 
GATGTTTCCT CTTTAATAGA GCATTCAATT AAACTGGAGG TCATCCACAT TAAGGATTGC 
AATAGCATGG AGAGCTTGAT TTCATCTTCT TTGTTCTGCC CTTCTCCAAC ATCATTGCCA 
TCTTATAATG GTGTATTTTC TACTCTTAAA GAGTTTAATT GTTATGGATG TAGTAGTATG 
AAGAAGTTGT TCCCTCTTGT CTTGCTGCCA AACCTCGTAA ACCTAGTAAA TATTACAGTT 
ATAGATTGTG CAAAAATGGA GGAGATAATA GGTGGAAGAA GATCAGATGA AGAAAACAGC 
AGCAACAACA CCGAATTCAA GCTCCCAAAG TTAAGATCTC TGGCATTGAG TTGGTTACCA 
AAACTGAAAA GAATTTGTAG TGCAAAACTG ATTTGCGATT CTCTCCAACA AATTGAAGTA 
AGTTACTGCA ATAGCATGGA GAGCTTGGTT CCATCTTCTT GGATTTCCCT CGTAAACCTA 
GAAAAGATTA CAGTTAGAGG ATGTCAGAAG ATGGAGGAGA TAATAGGTGG AAGAAGATCA 
GATGAAGAAA GCAGCAGCAA CAACACCGAA TTCAAACTCC CAAAGTTAAG ATCTCTGGCA 
TTGGAAGATT TACCAGAACT GAAAAGCATT TGTAGTGCAA AGCTGACGTG CGATTCTCTC 
CAACAAATTG AAGTATGGAA TTGCAACAGC ATGGAGAGCT TGGTTCCATC TTCTTGGATT 
TCCCTCGTAA ACCTAGAAAA GATTACAGTT AGAGGAGGTG AGAAAATGGA GGAGATAATA 
GGTGGAAGAA GATCAGATGA AGAAAGCAGC AGCACCGAAT TCAAACTCCC AAAGTTAAGA 
TCTCTGGCAT TGTTTAATTT ACCAGAACTG AAAAGCATTT GTAGTGCAAA GCTGACGTGC 
GATTCTCTCC AACAAATTGA AGTATGGAAT TGCAACAGCA TGGAGATCTT GGTTCCATCT 
TCTTGGATTT CCCTTGTAAA CCTAGAAAAG ATTACAGTTA GTGCATGTAA GAAGATGAAG 
GAGATAATAG GTGGAACAAG ATCAGATGAA GAAAGTAGCA GTAACAACAC CGAATTCAAA 
CTCCCAAAGT TAAGATCTCT GGCATTGAGT TGGTTACCAG AACTGAAAAG AATTTGTAGT 
GCAAAACTGA TTTGCGATTC TCTCAGAATG ATTGAAGTTT ATAAATGTCA GAAGCTGAAG 
AGGATGCCAT TGTGGAAGAA GGATTATACA TCCAAGAGAA TGGTGGGAGT CAGTAGTGGT 
GTGGGAGCAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024447688.1
CDS	901..4995
Translation	MSRPNDPFWEYAEKMDDGSMTCTFCGHCFSQGTSVTRIKLHLAGVKRRGVKICKNVPEEVRDAACDAVNNSPPEKKLKSVAGSSSNEVANAISASTQEQNNEVTHVEMAQQGGPFFTGELAWANDLIGEAELVQLERGSSHERPSINQTDEPRGDSSRPTDDQLCSPSVNNDANMNDVQNMVAVRIEPVPVQVLEQSNAEVDSLAGHAGRIQVGVHGMEQGAEEERICPNSAANGMENTGDGSSQHSRRLMIDAHDNTGEEATQRIDLVQRLEGETWGQMNAISAFLMGEEDVENNRGRLVQPGAACSSGGAACNTNKIKGDALPTRKMVGQAFEEHKKTISSLLMRNEVSSIGIYGMGGVGKTTLGTHIHNQLLERPETPVYWITVSHNTSIPRLQTSLAGRIGLDLSKVDEELHRAVALKKELMKKQKWILILDDLWKAFDLQKLGVPDQVEEGCKLILTSRSAKVCQQMKTQHTIKVQPISEKEAWTLFIERLGHDIAFSSEVEGIALNVVRECAGLPLGIITIAASMRGVDEPHEWRNTLKKLKESKYKEMEDEVFRLLRFSYDQLNDLALQQCLLYCALYPEDHRIEREELIGYLIDEEIIEGMRSRQAAFDEGRTMLDKLEKVCLLERACYGDHSTTVKMHDLIRDMAHQILQTNSPVMVGGYNDKLPDVDMWKENLVRVSLKHCYFEEIPSSHSPRCPNLSTLLLCDNPYLQFIADSFFTQLHGLKVLDLSRTEIIELPDSVSELVSLTALLLKQCEYLIHVPSLEKLTALRRLDLSGTWELEKIPQDMQCLSNLRYLRMDGCGVKEFPTGILPKLFHLQLFMLEGKTNYDYVQVTVKGKEVGCLRELENLVCNFEGQSDFVEYLNSRDKTRSLSTYDIFVGPLDEDFYSEMKRGYGKSKTVWLSSLCNNGDGNFQVMFPNDIQKLIIFNCSCDVSSLIEHSIKLEVIHIKDCNSMESLISSSLFCPSPTSLPSYNGVFSTLKEFNCYGCSSMKKLFPLVLLPNLVNLVNITVIDCAKMEEIIGGRRSDEENSSNNTEFKLPKLRSLALSWLPKLKRICSAKLICDSLQQIEVSYCNSMESLVPSSWISLVNLEKITVRGCQKMEEIIGGRRSDEESSSNNTEFKLPKLRSLALEDLPELKSICSAKLTCDSLQQIEVWNCNSMESLVPSSWISLVNLEKITVRGGEKMEEIIGGRRSDEESSSTEFKLPKLRSLALFNLPELKSICSAKLTCDSLQQIEVWNCNSMEILVPSSWISLVNLEKITVSACKKMKEIIGGTRSDEESSSNNTEFKLPKLRSLALSWLPELKRICSAKLICDSLRMIEVYKCQKLKRMPLWKKDYTSKRMVGVSSGVGAS

Target ORF information:

RefSeq Version	XM_024591920.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024591920.1

ORF Insert Sequence:

ATGAGTCGAC CAAATGATCC TTTTTGGGAA TATGCTGAAA AGATGGATGA TGGTAGCATG 
ACGTGTACGT TTTGTGGGCA TTGTTTTTCC CAAGGTACTT CCGTTACGAG GATCAAATTG 
CATTTGGCAG GAGTGAAACG GCGTGGTGTT AAAATTTGTA AAAATGTTCC AGAAGAAGTT 
CGAGATGCAG CTTGTGATGC AGTTAATAAT AGCCCTCCAG AAAAGAAACT TAAATCTGTT 
GCGGGCTCAA GCAGTAACGA GGTCGCTAAT GCAATTTCAG CTTCAACGCA AGAACAGAAC 
AATGAAGTGA CCCATGTAGA GATGGCACAA CAAGGAGGAC CTTTTTTCAC TGGAGAACTT 
GCATGGGCAA ATGATTTGAT TGGAGAGGCT GAACTTGTGC AGCTGGAGAG AGGTAGTTCT 
CATGAGAGGC CATCAATTAA TCAAACAGAT GAGCCTCGAG GAGATTCATC CCGACCAACA 
GATGATCAAC TCTGTTCTCC ATCAGTAAAC AATGATGCCA ATATGAACGA TGTGCAGAAC 
ATGGTTGCAG TCAGGATAGA ACCAGTACCG GTTCAAGTGT TGGAACAAAG CAATGCAGAA 
GTTGACAGTT TGGCAGGGCA TGCTGGAAGG ATACAAGTGG GAGTTCATGG CATGGAACAA 
GGTGCGGAGG AAGAGAGAAT TTGTCCGAAC TCAGCGGCAA ATGGCATGGA AAACACTGGT 
GATGGATCTA GTCAGCATTC CAGGAGACTC ATGATTGATG CACATGATAA TACAGGGGAA 
GAAGCTACAC AAAGAATAGA TCTAGTGCAA CGATTAGAAG GGGAAACTTG GGGTCAGATG 
AATGCAATCT CTGCTTTTTT AATGGGGGAG GAGGACGTGG AGAACAATCG TGGAAGATTA 
GTGCAGCCTG GAGCAGCTTG CTCTTCTGGA GGGGCTGCAT GCAACACAAA TAAGATTAAA 
GGAGATGCAC TGCCAACGAG AAAAATGGTG GGTCAAGCAT TCGAAGAACA TAAGAAGACT 
ATCTCATCTT TGTTGATGCG CAATGAAGTT TCAAGTATTG GTATCTATGG GATGGGCGGA 
GTGGGAAAGA CCACATTGGG GACACATATA CATAATCAGC TTCTAGAAAG ACCAGAGACT 
CCTGTTTACT GGATTACTGT TTCACATAAT ACCAGCATTC CTAGATTGCA GACAAGTCTC 
GCAGGACGAA TTGGTTTAGA TCTTTCCAAG GTAGATGAGG AGCTGCATAG AGCGGTAGCG 
TTGAAAAAAG AATTAATGAA GAAACAGAAA TGGATTCTGA TTTTAGATGA TTTGTGGAAG 
GCTTTTGACC TGCAAAAGCT GGGAGTTCCT GACCAAGTTG AGGAGGGATG CAAGCTGATT 
CTTACATCTC GATCAGCAAA GGTTTGTCAA CAGATGAAAA CCCAACACAC GATAAAAGTG 
CAGCCTATTT CGGAGAAAGA AGCTTGGACT TTGTTTATTG AGAGACTTGG ACATGACATA 
GCATTTTCTT CAGAAGTGGA AGGAATTGCT TTGAATGTTG TAAGGGAATG TGCTGGTTTG 
CCATTGGGAA TTATTACAAT AGCAGCAAGC ATGAGAGGAG TGGATGAGCC ACATGAGTGG 
AGGAATACAT TGAAGAAATT GAAAGAATCA AAATATAAGG AAATGGAAGA TGAGGTATTC 
CGATTATTGA GGTTTAGTTA TGATCAGCTA AATGATTTAG CATTACAACA ATGTCTCTTG 
TACTGTGCAT TGTATCCTGA AGATCATAGG ATTGAAAGGG AGGAGCTGAT AGGTTATTTG 
ATCGACGAGG AAATAATTGA AGGAATGAGG AGCAGGCAAG CAGCATTTGA CGAGGGCCGC 
ACGATGCTTG ATAAACTTGA AAAAGTCTGT TTATTGGAAA GAGCTTGTTA TGGTGATCAT 
AGTACAACTG TCAAGATGCA TGACTTGATT AGGGACATGG CCCACCAAAT ACTCCAAACA 
AACTCTCCAG TCATGGTTGG GGGATATAAT GATAAATTAC CAGATGTGGA TATGTGGAAA 
GAGAATCTAG TGAGAGTCTC TCTGAAGCAT TGTTATTTCG AGGAAATTCC TTCTAGTCAT 
TCACCAAGAT GTCCCAATCT TTCAACTCTA TTGCTGTGTG ATAATCCTTA TTTGCAATTT 
ATTGCAGATT CTTTTTTCAC ACAATTGCAT GGGCTCAAGG TTCTTGATCT GTCTCGTACA 
GAAATCATAG AATTGCCTGA TTCTGTCTCT GAACTGGTGA GTCTCACTGC ATTATTGCTC 
AAACAATGTG AATATTTAAT ACATGTACCA TCATTAGAAA AGCTCACAGC ACTGAGGAGG 
CTAGATCTCT CTGGTACTTG GGAACTTGAA AAGATACCTC AAGACATGCA ATGTCTATCT 
AACCTGAGGT ATCTGAGGAT GGATGGATGT GGTGTAAAGG AGTTTCCTAC TGGGATATTA 
CCAAAACTCT TTCACCTACA ACTCTTCATG TTAGAGGGGA AGACTAATTA TGACTACGTT 
CAAGTAACAG TTAAGGGAAA GGAAGTAGGA TGCTTGAGGG AGTTGGAAAA TTTGGTATGC 
AATTTTGAAG GTCAATCTGA CTTTGTGGAG TATCTCAATT CTCGGGATAA GACCCGATCA 
CTAAGTACAT ACGATATTTT TGTAGGACCA CTGGATGAAG ATTTTTATAG TGAAATGAAG 
CGTGGTTATG GCAAAAGTAA AACAGTTTGG TTGAGTAGTT TGTGTAACAA TGGAGATGGA 
AATTTTCAAG TAATGTTCCC AAATGACATT CAAAAACTGA TTATTTTTAA TTGTTCATGT 
GATGTTTCCT CTTTAATAGA GCATTCAATT AAACTGGAGG TCATCCACAT TAAGGATTGC 
AATAGCATGG AGAGCTTGAT TTCATCTTCT TTGTTCTGCC CTTCTCCAAC ATCATTGCCA 
TCTTATAATG GTGTATTTTC TACTCTTAAA GAGTTTAATT GTTATGGATG TAGTAGTATG 
AAGAAGTTGT TCCCTCTTGT CTTGCTGCCA AACCTCGTAA ACCTAGTAAA TATTACAGTT 
ATAGATTGTG CAAAAATGGA GGAGATAATA GGTGGAAGAA GATCAGATGA AGAAAACAGC 
AGCAACAACA CCGAATTCAA GCTCCCAAAG TTAAGATCTC TGGCATTGAG TTGGTTACCA 
AAACTGAAAA GAATTTGTAG TGCAAAACTG ATTTGCGATT CTCTCCAACA AATTGAAGTA 
AGTTACTGCA ATAGCATGGA GAGCTTGGTT CCATCTTCTT GGATTTCCCT CGTAAACCTA 
GAAAAGATTA CAGTTAGAGG ATGTCAGAAG ATGGAGGAGA TAATAGGTGG AAGAAGATCA 
GATGAAGAAA GCAGCAGCAA CAACACCGAA TTCAAACTCC CAAAGTTAAG ATCTCTGGCA 
TTGGAAGATT TACCAGAACT GAAAAGCATT TGTAGTGCAA AGCTGACGTG CGATTCTCTC 
CAACAAATTG AAGTATGGAA TTGCAACAGC ATGGAGAGCT TGGTTCCATC TTCTTGGATT 
TCCCTCGTAA ACCTAGAAAA GATTACAGTT AGAGGAGGTG AGAAAATGGA GGAGATAATA 
GGTGGAAGAA GATCAGATGA AGAAAGCAGC AGCACCGAAT TCAAACTCCC AAAGTTAAGA 
TCTCTGGCAT TGTTTAATTT ACCAGAACTG AAAAGCATTT GTAGTGCAAA GCTGACGTGC 
GATTCTCTCC AACAAATTGA AGTATGGAAT TGCAACAGCA TGGAGATCTT GGTTCCATCT 
TCTTGGATTT CCCTTGTAAA CCTAGAAAAG ATTACAGTTA GTGCATGTAA GAAGATGAAG 
GAGATAATAG GTGGAACAAG ATCAGATGAA GAAAGTAGCA GTAACAACAC CGAATTCAAA 
CTCCCAAAGT TAAGATCTCT GGCATTGAGT TGGTTACCAG AACTGAAAAG AATTTGTAGT 
GCAAAACTGA TTTGCGATTC TCTCAGAATG ATTGAAGTTT ATAAATGTCA GAAGCTGAAG 
AGGATGCCAT TGTGGAAGAA GGATTATACA TCCAAGAGAA TGGTGGGAGT CAGTAGTGGT 
GTGGGAGCAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18107958 ( XM_024591911.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024447679.1 Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X2, mRNA.

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CloneID	OPa418670
Clone ID Related Accession (Same CDS sequence)	XM_024591920.1 , XM_024591911.1 , XM_024591912.1 , XM_024591917.1 , XM_024591913.1 , XM_024591916.1 , XM_024591918.1 , XM_024591919.1 , XM_024591910.1 , XM_024591914.1
Accession Version	XM_024591911.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	probable disease resistance protein At4g27220 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020167869.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCGAC CAAATGATCC TTTTTGGGAA TATGCTGAAA AGATGGATGA TGGTAGCATG 
ACGTGTACGT TTTGTGGGCA TTGTTTTTCC CAAGGTACTT CCGTTACGAG GATCAAATTG 
CATTTGGCAG GAGTGAAACG GCGTGGTGTT AAAATTTGTA AAAATGTTCC AGAAGAAGTT 
CGAGATGCAG CTTGTGATGC AGTTAATAAT AGCCCTCCAG AAAAGAAACT TAAATCTGTT 
GCGGGCTCAA GCAGTAACGA GGTCGCTAAT GCAATTTCAG CTTCAACGCA AGAACAGAAC 
AATGAAGTGA CCCATGTAGA GATGGCACAA CAAGGAGGAC CTTTTTTCAC TGGAGAACTT 
GCATGGGCAA ATGATTTGAT TGGAGAGGCT GAACTTGTGC AGCTGGAGAG AGGTAGTTCT 
CATGAGAGGC CATCAATTAA TCAAACAGAT GAGCCTCGAG GAGATTCATC CCGACCAACA 
GATGATCAAC TCTGTTCTCC ATCAGTAAAC AATGATGCCA ATATGAACGA TGTGCAGAAC 
ATGGTTGCAG TCAGGATAGA ACCAGTACCG GTTCAAGTGT TGGAACAAAG CAATGCAGAA 
GTTGACAGTT TGGCAGGGCA TGCTGGAAGG ATACAAGTGG GAGTTCATGG CATGGAACAA 
GGTGCGGAGG AAGAGAGAAT TTGTCCGAAC TCAGCGGCAA ATGGCATGGA AAACACTGGT 
GATGGATCTA GTCAGCATTC CAGGAGACTC ATGATTGATG CACATGATAA TACAGGGGAA 
GAAGCTACAC AAAGAATAGA TCTAGTGCAA CGATTAGAAG GGGAAACTTG GGGTCAGATG 
AATGCAATCT CTGCTTTTTT AATGGGGGAG GAGGACGTGG AGAACAATCG TGGAAGATTA 
GTGCAGCCTG GAGCAGCTTG CTCTTCTGGA GGGGCTGCAT GCAACACAAA TAAGATTAAA 
GGAGATGCAC TGCCAACGAG AAAAATGGTG GGTCAAGCAT TCGAAGAACA TAAGAAGACT 
ATCTCATCTT TGTTGATGCG CAATGAAGTT TCAAGTATTG GTATCTATGG GATGGGCGGA 
GTGGGAAAGA CCACATTGGG GACACATATA CATAATCAGC TTCTAGAAAG ACCAGAGACT 
CCTGTTTACT GGATTACTGT TTCACATAAT ACCAGCATTC CTAGATTGCA GACAAGTCTC 
GCAGGACGAA TTGGTTTAGA TCTTTCCAAG GTAGATGAGG AGCTGCATAG AGCGGTAGCG 
TTGAAAAAAG AATTAATGAA GAAACAGAAA TGGATTCTGA TTTTAGATGA TTTGTGGAAG 
GCTTTTGACC TGCAAAAGCT GGGAGTTCCT GACCAAGTTG AGGAGGGATG CAAGCTGATT 
CTTACATCTC GATCAGCAAA GGTTTGTCAA CAGATGAAAA CCCAACACAC GATAAAAGTG 
CAGCCTATTT CGGAGAAAGA AGCTTGGACT TTGTTTATTG AGAGACTTGG ACATGACATA 
GCATTTTCTT CAGAAGTGGA AGGAATTGCT TTGAATGTTG TAAGGGAATG TGCTGGTTTG 
CCATTGGGAA TTATTACAAT AGCAGCAAGC ATGAGAGGAG TGGATGAGCC ACATGAGTGG 
AGGAATACAT TGAAGAAATT GAAAGAATCA AAATATAAGG AAATGGAAGA TGAGGTATTC 
CGATTATTGA GGTTTAGTTA TGATCAGCTA AATGATTTAG CATTACAACA ATGTCTCTTG 
TACTGTGCAT TGTATCCTGA AGATCATAGG ATTGAAAGGG AGGAGCTGAT AGGTTATTTG 
ATCGACGAGG AAATAATTGA AGGAATGAGG AGCAGGCAAG CAGCATTTGA CGAGGGCCGC 
ACGATGCTTG ATAAACTTGA AAAAGTCTGT TTATTGGAAA GAGCTTGTTA TGGTGATCAT 
AGTACAACTG TCAAGATGCA TGACTTGATT AGGGACATGG CCCACCAAAT ACTCCAAACA 
AACTCTCCAG TCATGGTTGG GGGATATAAT GATAAATTAC CAGATGTGGA TATGTGGAAA 
GAGAATCTAG TGAGAGTCTC TCTGAAGCAT TGTTATTTCG AGGAAATTCC TTCTAGTCAT 
TCACCAAGAT GTCCCAATCT TTCAACTCTA TTGCTGTGTG ATAATCCTTA TTTGCAATTT 
ATTGCAGATT CTTTTTTCAC ACAATTGCAT GGGCTCAAGG TTCTTGATCT GTCTCGTACA 
GAAATCATAG AATTGCCTGA TTCTGTCTCT GAACTGGTGA GTCTCACTGC ATTATTGCTC 
AAACAATGTG AATATTTAAT ACATGTACCA TCATTAGAAA AGCTCACAGC ACTGAGGAGG 
CTAGATCTCT CTGGTACTTG GGAACTTGAA AAGATACCTC AAGACATGCA ATGTCTATCT 
AACCTGAGGT ATCTGAGGAT GGATGGATGT GGTGTAAAGG AGTTTCCTAC TGGGATATTA 
CCAAAACTCT TTCACCTACA ACTCTTCATG TTAGAGGGGA AGACTAATTA TGACTACGTT 
CAAGTAACAG TTAAGGGAAA GGAAGTAGGA TGCTTGAGGG AGTTGGAAAA TTTGGTATGC 
AATTTTGAAG GTCAATCTGA CTTTGTGGAG TATCTCAATT CTCGGGATAA GACCCGATCA 
CTAAGTACAT ACGATATTTT TGTAGGACCA CTGGATGAAG ATTTTTATAG TGAAATGAAG 
CGTGGTTATG GCAAAAGTAA AACAGTTTGG TTGAGTAGTT TGTGTAACAA TGGAGATGGA 
AATTTTCAAG TAATGTTCCC AAATGACATT CAAAAACTGA TTATTTTTAA TTGTTCATGT 
GATGTTTCCT CTTTAATAGA GCATTCAATT AAACTGGAGG TCATCCACAT TAAGGATTGC 
AATAGCATGG AGAGCTTGAT TTCATCTTCT TTGTTCTGCC CTTCTCCAAC ATCATTGCCA 
TCTTATAATG GTGTATTTTC TACTCTTAAA GAGTTTAATT GTTATGGATG TAGTAGTATG 
AAGAAGTTGT TCCCTCTTGT CTTGCTGCCA AACCTCGTAA ACCTAGTAAA TATTACAGTT 
ATAGATTGTG CAAAAATGGA GGAGATAATA GGTGGAAGAA GATCAGATGA AGAAAACAGC 
AGCAACAACA CCGAATTCAA GCTCCCAAAG TTAAGATCTC TGGCATTGAG TTGGTTACCA 
AAACTGAAAA GAATTTGTAG TGCAAAACTG ATTTGCGATT CTCTCCAACA AATTGAAGTA 
AGTTACTGCA ATAGCATGGA GAGCTTGGTT CCATCTTCTT GGATTTCCCT CGTAAACCTA 
GAAAAGATTA CAGTTAGAGG ATGTCAGAAG ATGGAGGAGA TAATAGGTGG AAGAAGATCA 
GATGAAGAAA GCAGCAGCAA CAACACCGAA TTCAAACTCC CAAAGTTAAG ATCTCTGGCA 
TTGGAAGATT TACCAGAACT GAAAAGCATT TGTAGTGCAA AGCTGACGTG CGATTCTCTC 
CAACAAATTG AAGTATGGAA TTGCAACAGC ATGGAGAGCT TGGTTCCATC TTCTTGGATT 
TCCCTCGTAA ACCTAGAAAA GATTACAGTT AGAGGAGGTG AGAAAATGGA GGAGATAATA 
GGTGGAAGAA GATCAGATGA AGAAAGCAGC AGCACCGAAT TCAAACTCCC AAAGTTAAGA 
TCTCTGGCAT TGTTTAATTT ACCAGAACTG AAAAGCATTT GTAGTGCAAA GCTGACGTGC 
GATTCTCTCC AACAAATTGA AGTATGGAAT TGCAACAGCA TGGAGATCTT GGTTCCATCT 
TCTTGGATTT CCCTTGTAAA CCTAGAAAAG ATTACAGTTA GTGCATGTAA GAAGATGAAG 
GAGATAATAG GTGGAACAAG ATCAGATGAA GAAAGTAGCA GTAACAACAC CGAATTCAAA 
CTCCCAAAGT TAAGATCTCT GGCATTGAGT TGGTTACCAG AACTGAAAAG AATTTGTAGT 
GCAAAACTGA TTTGCGATTC TCTCAGAATG ATTGAAGTTT ATAAATGTCA GAAGCTGAAG 
AGGATGCCAT TGTGGAAGAA GGATTATACA TCCAAGAGAA TGGTGGGAGT CAGTAGTGGT 
GTGGGAGCAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024447679.1
CDS	736..4830
Translation	MSRPNDPFWEYAEKMDDGSMTCTFCGHCFSQGTSVTRIKLHLAGVKRRGVKICKNVPEEVRDAACDAVNNSPPEKKLKSVAGSSSNEVANAISASTQEQNNEVTHVEMAQQGGPFFTGELAWANDLIGEAELVQLERGSSHERPSINQTDEPRGDSSRPTDDQLCSPSVNNDANMNDVQNMVAVRIEPVPVQVLEQSNAEVDSLAGHAGRIQVGVHGMEQGAEEERICPNSAANGMENTGDGSSQHSRRLMIDAHDNTGEEATQRIDLVQRLEGETWGQMNAISAFLMGEEDVENNRGRLVQPGAACSSGGAACNTNKIKGDALPTRKMVGQAFEEHKKTISSLLMRNEVSSIGIYGMGGVGKTTLGTHIHNQLLERPETPVYWITVSHNTSIPRLQTSLAGRIGLDLSKVDEELHRAVALKKELMKKQKWILILDDLWKAFDLQKLGVPDQVEEGCKLILTSRSAKVCQQMKTQHTIKVQPISEKEAWTLFIERLGHDIAFSSEVEGIALNVVRECAGLPLGIITIAASMRGVDEPHEWRNTLKKLKESKYKEMEDEVFRLLRFSYDQLNDLALQQCLLYCALYPEDHRIEREELIGYLIDEEIIEGMRSRQAAFDEGRTMLDKLEKVCLLERACYGDHSTTVKMHDLIRDMAHQILQTNSPVMVGGYNDKLPDVDMWKENLVRVSLKHCYFEEIPSSHSPRCPNLSTLLLCDNPYLQFIADSFFTQLHGLKVLDLSRTEIIELPDSVSELVSLTALLLKQCEYLIHVPSLEKLTALRRLDLSGTWELEKIPQDMQCLSNLRYLRMDGCGVKEFPTGILPKLFHLQLFMLEGKTNYDYVQVTVKGKEVGCLRELENLVCNFEGQSDFVEYLNSRDKTRSLSTYDIFVGPLDEDFYSEMKRGYGKSKTVWLSSLCNNGDGNFQVMFPNDIQKLIIFNCSCDVSSLIEHSIKLEVIHIKDCNSMESLISSSLFCPSPTSLPSYNGVFSTLKEFNCYGCSSMKKLFPLVLLPNLVNLVNITVIDCAKMEEIIGGRRSDEENSSNNTEFKLPKLRSLALSWLPKLKRICSAKLICDSLQQIEVSYCNSMESLVPSSWISLVNLEKITVRGCQKMEEIIGGRRSDEESSSNNTEFKLPKLRSLALEDLPELKSICSAKLTCDSLQQIEVWNCNSMESLVPSSWISLVNLEKITVRGGEKMEEIIGGRRSDEESSSTEFKLPKLRSLALFNLPELKSICSAKLTCDSLQQIEVWNCNSMEILVPSSWISLVNLEKITVSACKKMKEIIGGTRSDEESSSNNTEFKLPKLRSLALSWLPELKRICSAKLICDSLRMIEVYKCQKLKRMPLWKKDYTSKRMVGVSSGVGAS

Target ORF information:

RefSeq Version	XM_024591911.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024591911.1

ORF Insert Sequence:

ATGAGTCGAC CAAATGATCC TTTTTGGGAA TATGCTGAAA AGATGGATGA TGGTAGCATG 
ACGTGTACGT TTTGTGGGCA TTGTTTTTCC CAAGGTACTT CCGTTACGAG GATCAAATTG 
CATTTGGCAG GAGTGAAACG GCGTGGTGTT AAAATTTGTA AAAATGTTCC AGAAGAAGTT 
CGAGATGCAG CTTGTGATGC AGTTAATAAT AGCCCTCCAG AAAAGAAACT TAAATCTGTT 
GCGGGCTCAA GCAGTAACGA GGTCGCTAAT GCAATTTCAG CTTCAACGCA AGAACAGAAC 
AATGAAGTGA CCCATGTAGA GATGGCACAA CAAGGAGGAC CTTTTTTCAC TGGAGAACTT 
GCATGGGCAA ATGATTTGAT TGGAGAGGCT GAACTTGTGC AGCTGGAGAG AGGTAGTTCT 
CATGAGAGGC CATCAATTAA TCAAACAGAT GAGCCTCGAG GAGATTCATC CCGACCAACA 
GATGATCAAC TCTGTTCTCC ATCAGTAAAC AATGATGCCA ATATGAACGA TGTGCAGAAC 
ATGGTTGCAG TCAGGATAGA ACCAGTACCG GTTCAAGTGT TGGAACAAAG CAATGCAGAA 
GTTGACAGTT TGGCAGGGCA TGCTGGAAGG ATACAAGTGG GAGTTCATGG CATGGAACAA 
GGTGCGGAGG AAGAGAGAAT TTGTCCGAAC TCAGCGGCAA ATGGCATGGA AAACACTGGT 
GATGGATCTA GTCAGCATTC CAGGAGACTC ATGATTGATG CACATGATAA TACAGGGGAA 
GAAGCTACAC AAAGAATAGA TCTAGTGCAA CGATTAGAAG GGGAAACTTG GGGTCAGATG 
AATGCAATCT CTGCTTTTTT AATGGGGGAG GAGGACGTGG AGAACAATCG TGGAAGATTA 
GTGCAGCCTG GAGCAGCTTG CTCTTCTGGA GGGGCTGCAT GCAACACAAA TAAGATTAAA 
GGAGATGCAC TGCCAACGAG AAAAATGGTG GGTCAAGCAT TCGAAGAACA TAAGAAGACT 
ATCTCATCTT TGTTGATGCG CAATGAAGTT TCAAGTATTG GTATCTATGG GATGGGCGGA 
GTGGGAAAGA CCACATTGGG GACACATATA CATAATCAGC TTCTAGAAAG ACCAGAGACT 
CCTGTTTACT GGATTACTGT TTCACATAAT ACCAGCATTC CTAGATTGCA GACAAGTCTC 
GCAGGACGAA TTGGTTTAGA TCTTTCCAAG GTAGATGAGG AGCTGCATAG AGCGGTAGCG 
TTGAAAAAAG AATTAATGAA GAAACAGAAA TGGATTCTGA TTTTAGATGA TTTGTGGAAG 
GCTTTTGACC TGCAAAAGCT GGGAGTTCCT GACCAAGTTG AGGAGGGATG CAAGCTGATT 
CTTACATCTC GATCAGCAAA GGTTTGTCAA CAGATGAAAA CCCAACACAC GATAAAAGTG 
CAGCCTATTT CGGAGAAAGA AGCTTGGACT TTGTTTATTG AGAGACTTGG ACATGACATA 
GCATTTTCTT CAGAAGTGGA AGGAATTGCT TTGAATGTTG TAAGGGAATG TGCTGGTTTG 
CCATTGGGAA TTATTACAAT AGCAGCAAGC ATGAGAGGAG TGGATGAGCC ACATGAGTGG 
AGGAATACAT TGAAGAAATT GAAAGAATCA AAATATAAGG AAATGGAAGA TGAGGTATTC 
CGATTATTGA GGTTTAGTTA TGATCAGCTA AATGATTTAG CATTACAACA ATGTCTCTTG 
TACTGTGCAT TGTATCCTGA AGATCATAGG ATTGAAAGGG AGGAGCTGAT AGGTTATTTG 
ATCGACGAGG AAATAATTGA AGGAATGAGG AGCAGGCAAG CAGCATTTGA CGAGGGCCGC 
ACGATGCTTG ATAAACTTGA AAAAGTCTGT TTATTGGAAA GAGCTTGTTA TGGTGATCAT 
AGTACAACTG TCAAGATGCA TGACTTGATT AGGGACATGG CCCACCAAAT ACTCCAAACA 
AACTCTCCAG TCATGGTTGG GGGATATAAT GATAAATTAC CAGATGTGGA TATGTGGAAA 
GAGAATCTAG TGAGAGTCTC TCTGAAGCAT TGTTATTTCG AGGAAATTCC TTCTAGTCAT 
TCACCAAGAT GTCCCAATCT TTCAACTCTA TTGCTGTGTG ATAATCCTTA TTTGCAATTT 
ATTGCAGATT CTTTTTTCAC ACAATTGCAT GGGCTCAAGG TTCTTGATCT GTCTCGTACA 
GAAATCATAG AATTGCCTGA TTCTGTCTCT GAACTGGTGA GTCTCACTGC ATTATTGCTC 
AAACAATGTG AATATTTAAT ACATGTACCA TCATTAGAAA AGCTCACAGC ACTGAGGAGG 
CTAGATCTCT CTGGTACTTG GGAACTTGAA AAGATACCTC AAGACATGCA ATGTCTATCT 
AACCTGAGGT ATCTGAGGAT GGATGGATGT GGTGTAAAGG AGTTTCCTAC TGGGATATTA 
CCAAAACTCT TTCACCTACA ACTCTTCATG TTAGAGGGGA AGACTAATTA TGACTACGTT 
CAAGTAACAG TTAAGGGAAA GGAAGTAGGA TGCTTGAGGG AGTTGGAAAA TTTGGTATGC 
AATTTTGAAG GTCAATCTGA CTTTGTGGAG TATCTCAATT CTCGGGATAA GACCCGATCA 
CTAAGTACAT ACGATATTTT TGTAGGACCA CTGGATGAAG ATTTTTATAG TGAAATGAAG 
CGTGGTTATG GCAAAAGTAA AACAGTTTGG TTGAGTAGTT TGTGTAACAA TGGAGATGGA 
AATTTTCAAG TAATGTTCCC AAATGACATT CAAAAACTGA TTATTTTTAA TTGTTCATGT 
GATGTTTCCT CTTTAATAGA GCATTCAATT AAACTGGAGG TCATCCACAT TAAGGATTGC 
AATAGCATGG AGAGCTTGAT TTCATCTTCT TTGTTCTGCC CTTCTCCAAC ATCATTGCCA 
TCTTATAATG GTGTATTTTC TACTCTTAAA GAGTTTAATT GTTATGGATG TAGTAGTATG 
AAGAAGTTGT TCCCTCTTGT CTTGCTGCCA AACCTCGTAA ACCTAGTAAA TATTACAGTT 
ATAGATTGTG CAAAAATGGA GGAGATAATA GGTGGAAGAA GATCAGATGA AGAAAACAGC 
AGCAACAACA CCGAATTCAA GCTCCCAAAG TTAAGATCTC TGGCATTGAG TTGGTTACCA 
AAACTGAAAA GAATTTGTAG TGCAAAACTG ATTTGCGATT CTCTCCAACA AATTGAAGTA 
AGTTACTGCA ATAGCATGGA GAGCTTGGTT CCATCTTCTT GGATTTCCCT CGTAAACCTA 
GAAAAGATTA CAGTTAGAGG ATGTCAGAAG ATGGAGGAGA TAATAGGTGG AAGAAGATCA 
GATGAAGAAA GCAGCAGCAA CAACACCGAA TTCAAACTCC CAAAGTTAAG ATCTCTGGCA 
TTGGAAGATT TACCAGAACT GAAAAGCATT TGTAGTGCAA AGCTGACGTG CGATTCTCTC 
CAACAAATTG AAGTATGGAA TTGCAACAGC ATGGAGAGCT TGGTTCCATC TTCTTGGATT 
TCCCTCGTAA ACCTAGAAAA GATTACAGTT AGAGGAGGTG AGAAAATGGA GGAGATAATA 
GGTGGAAGAA GATCAGATGA AGAAAGCAGC AGCACCGAAT TCAAACTCCC AAAGTTAAGA 
TCTCTGGCAT TGTTTAATTT ACCAGAACTG AAAAGCATTT GTAGTGCAAA GCTGACGTGC 
GATTCTCTCC AACAAATTGA AGTATGGAAT TGCAACAGCA TGGAGATCTT GGTTCCATCT 
TCTTGGATTT CCCTTGTAAA CCTAGAAAAG ATTACAGTTA GTGCATGTAA GAAGATGAAG 
GAGATAATAG GTGGAACAAG ATCAGATGAA GAAAGTAGCA GTAACAACAC CGAATTCAAA 
CTCCCAAAGT TAAGATCTCT GGCATTGAGT TGGTTACCAG AACTGAAAAG AATTTGTAGT 
GCAAAACTGA TTTGCGATTC TCTCAGAATG ATTGAAGTTT ATAAATGTCA GAAGCTGAAG 
AGGATGCCAT TGTGGAAGAA GGATTATACA TCCAAGAGAA TGGTGGGAGT CAGTAGTGGT 
GTGGGAGCAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18107958 ( XM_024591912.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024447680.1 Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPa418670
Clone ID Related Accession (Same CDS sequence)	XM_024591920.1 , XM_024591911.1 , XM_024591912.1 , XM_024591917.1 , XM_024591913.1 , XM_024591916.1 , XM_024591918.1 , XM_024591919.1 , XM_024591910.1 , XM_024591914.1
Accession Version	XM_024591912.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	probable disease resistance protein At4g27220 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020167869.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCGAC CAAATGATCC TTTTTGGGAA TATGCTGAAA AGATGGATGA TGGTAGCATG 
ACGTGTACGT TTTGTGGGCA TTGTTTTTCC CAAGGTACTT CCGTTACGAG GATCAAATTG 
CATTTGGCAG GAGTGAAACG GCGTGGTGTT AAAATTTGTA AAAATGTTCC AGAAGAAGTT 
CGAGATGCAG CTTGTGATGC AGTTAATAAT AGCCCTCCAG AAAAGAAACT TAAATCTGTT 
GCGGGCTCAA GCAGTAACGA GGTCGCTAAT GCAATTTCAG CTTCAACGCA AGAACAGAAC 
AATGAAGTGA CCCATGTAGA GATGGCACAA CAAGGAGGAC CTTTTTTCAC TGGAGAACTT 
GCATGGGCAA ATGATTTGAT TGGAGAGGCT GAACTTGTGC AGCTGGAGAG AGGTAGTTCT 
CATGAGAGGC CATCAATTAA TCAAACAGAT GAGCCTCGAG GAGATTCATC CCGACCAACA 
GATGATCAAC TCTGTTCTCC ATCAGTAAAC AATGATGCCA ATATGAACGA TGTGCAGAAC 
ATGGTTGCAG TCAGGATAGA ACCAGTACCG GTTCAAGTGT TGGAACAAAG CAATGCAGAA 
GTTGACAGTT TGGCAGGGCA TGCTGGAAGG ATACAAGTGG GAGTTCATGG CATGGAACAA 
GGTGCGGAGG AAGAGAGAAT TTGTCCGAAC TCAGCGGCAA ATGGCATGGA AAACACTGGT 
GATGGATCTA GTCAGCATTC CAGGAGACTC ATGATTGATG CACATGATAA TACAGGGGAA 
GAAGCTACAC AAAGAATAGA TCTAGTGCAA CGATTAGAAG GGGAAACTTG GGGTCAGATG 
AATGCAATCT CTGCTTTTTT AATGGGGGAG GAGGACGTGG AGAACAATCG TGGAAGATTA 
GTGCAGCCTG GAGCAGCTTG CTCTTCTGGA GGGGCTGCAT GCAACACAAA TAAGATTAAA 
GGAGATGCAC TGCCAACGAG AAAAATGGTG GGTCAAGCAT TCGAAGAACA TAAGAAGACT 
ATCTCATCTT TGTTGATGCG CAATGAAGTT TCAAGTATTG GTATCTATGG GATGGGCGGA 
GTGGGAAAGA CCACATTGGG GACACATATA CATAATCAGC TTCTAGAAAG ACCAGAGACT 
CCTGTTTACT GGATTACTGT TTCACATAAT ACCAGCATTC CTAGATTGCA GACAAGTCTC 
GCAGGACGAA TTGGTTTAGA TCTTTCCAAG GTAGATGAGG AGCTGCATAG AGCGGTAGCG 
TTGAAAAAAG AATTAATGAA GAAACAGAAA TGGATTCTGA TTTTAGATGA TTTGTGGAAG 
GCTTTTGACC TGCAAAAGCT GGGAGTTCCT GACCAAGTTG AGGAGGGATG CAAGCTGATT 
CTTACATCTC GATCAGCAAA GGTTTGTCAA CAGATGAAAA CCCAACACAC GATAAAAGTG 
CAGCCTATTT CGGAGAAAGA AGCTTGGACT TTGTTTATTG AGAGACTTGG ACATGACATA 
GCATTTTCTT CAGAAGTGGA AGGAATTGCT TTGAATGTTG TAAGGGAATG TGCTGGTTTG 
CCATTGGGAA TTATTACAAT AGCAGCAAGC ATGAGAGGAG TGGATGAGCC ACATGAGTGG 
AGGAATACAT TGAAGAAATT GAAAGAATCA AAATATAAGG AAATGGAAGA TGAGGTATTC 
CGATTATTGA GGTTTAGTTA TGATCAGCTA AATGATTTAG CATTACAACA ATGTCTCTTG 
TACTGTGCAT TGTATCCTGA AGATCATAGG ATTGAAAGGG AGGAGCTGAT AGGTTATTTG 
ATCGACGAGG AAATAATTGA AGGAATGAGG AGCAGGCAAG CAGCATTTGA CGAGGGCCGC 
ACGATGCTTG ATAAACTTGA AAAAGTCTGT TTATTGGAAA GAGCTTGTTA TGGTGATCAT 
AGTACAACTG TCAAGATGCA TGACTTGATT AGGGACATGG CCCACCAAAT ACTCCAAACA 
AACTCTCCAG TCATGGTTGG GGGATATAAT GATAAATTAC CAGATGTGGA TATGTGGAAA 
GAGAATCTAG TGAGAGTCTC TCTGAAGCAT TGTTATTTCG AGGAAATTCC TTCTAGTCAT 
TCACCAAGAT GTCCCAATCT TTCAACTCTA TTGCTGTGTG ATAATCCTTA TTTGCAATTT 
ATTGCAGATT CTTTTTTCAC ACAATTGCAT GGGCTCAAGG TTCTTGATCT GTCTCGTACA 
GAAATCATAG AATTGCCTGA TTCTGTCTCT GAACTGGTGA GTCTCACTGC ATTATTGCTC 
AAACAATGTG AATATTTAAT ACATGTACCA TCATTAGAAA AGCTCACAGC ACTGAGGAGG 
CTAGATCTCT CTGGTACTTG GGAACTTGAA AAGATACCTC AAGACATGCA ATGTCTATCT 
AACCTGAGGT ATCTGAGGAT GGATGGATGT GGTGTAAAGG AGTTTCCTAC TGGGATATTA 
CCAAAACTCT TTCACCTACA ACTCTTCATG TTAGAGGGGA AGACTAATTA TGACTACGTT 
CAAGTAACAG TTAAGGGAAA GGAAGTAGGA TGCTTGAGGG AGTTGGAAAA TTTGGTATGC 
AATTTTGAAG GTCAATCTGA CTTTGTGGAG TATCTCAATT CTCGGGATAA GACCCGATCA 
CTAAGTACAT ACGATATTTT TGTAGGACCA CTGGATGAAG ATTTTTATAG TGAAATGAAG 
CGTGGTTATG GCAAAAGTAA AACAGTTTGG TTGAGTAGTT TGTGTAACAA TGGAGATGGA 
AATTTTCAAG TAATGTTCCC AAATGACATT CAAAAACTGA TTATTTTTAA TTGTTCATGT 
GATGTTTCCT CTTTAATAGA GCATTCAATT AAACTGGAGG TCATCCACAT TAAGGATTGC 
AATAGCATGG AGAGCTTGAT TTCATCTTCT TTGTTCTGCC CTTCTCCAAC ATCATTGCCA 
TCTTATAATG GTGTATTTTC TACTCTTAAA GAGTTTAATT GTTATGGATG TAGTAGTATG 
AAGAAGTTGT TCCCTCTTGT CTTGCTGCCA AACCTCGTAA ACCTAGTAAA TATTACAGTT 
ATAGATTGTG CAAAAATGGA GGAGATAATA GGTGGAAGAA GATCAGATGA AGAAAACAGC 
AGCAACAACA CCGAATTCAA GCTCCCAAAG TTAAGATCTC TGGCATTGAG TTGGTTACCA 
AAACTGAAAA GAATTTGTAG TGCAAAACTG ATTTGCGATT CTCTCCAACA AATTGAAGTA 
AGTTACTGCA ATAGCATGGA GAGCTTGGTT CCATCTTCTT GGATTTCCCT CGTAAACCTA 
GAAAAGATTA CAGTTAGAGG ATGTCAGAAG ATGGAGGAGA TAATAGGTGG AAGAAGATCA 
GATGAAGAAA GCAGCAGCAA CAACACCGAA TTCAAACTCC CAAAGTTAAG ATCTCTGGCA 
TTGGAAGATT TACCAGAACT GAAAAGCATT TGTAGTGCAA AGCTGACGTG CGATTCTCTC 
CAACAAATTG AAGTATGGAA TTGCAACAGC ATGGAGAGCT TGGTTCCATC TTCTTGGATT 
TCCCTCGTAA ACCTAGAAAA GATTACAGTT AGAGGAGGTG AGAAAATGGA GGAGATAATA 
GGTGGAAGAA GATCAGATGA AGAAAGCAGC AGCACCGAAT TCAAACTCCC AAAGTTAAGA 
TCTCTGGCAT TGTTTAATTT ACCAGAACTG AAAAGCATTT GTAGTGCAAA GCTGACGTGC 
GATTCTCTCC AACAAATTGA AGTATGGAAT TGCAACAGCA TGGAGATCTT GGTTCCATCT 
TCTTGGATTT CCCTTGTAAA CCTAGAAAAG ATTACAGTTA GTGCATGTAA GAAGATGAAG 
GAGATAATAG GTGGAACAAG ATCAGATGAA GAAAGTAGCA GTAACAACAC CGAATTCAAA 
CTCCCAAAGT TAAGATCTCT GGCATTGAGT TGGTTACCAG AACTGAAAAG AATTTGTAGT 
GCAAAACTGA TTTGCGATTC TCTCAGAATG ATTGAAGTTT ATAAATGTCA GAAGCTGAAG 
AGGATGCCAT TGTGGAAGAA GGATTATACA TCCAAGAGAA TGGTGGGAGT CAGTAGTGGT 
GTGGGAGCAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024447680.1
CDS	901..4995
Translation	MSRPNDPFWEYAEKMDDGSMTCTFCGHCFSQGTSVTRIKLHLAGVKRRGVKICKNVPEEVRDAACDAVNNSPPEKKLKSVAGSSSNEVANAISASTQEQNNEVTHVEMAQQGGPFFTGELAWANDLIGEAELVQLERGSSHERPSINQTDEPRGDSSRPTDDQLCSPSVNNDANMNDVQNMVAVRIEPVPVQVLEQSNAEVDSLAGHAGRIQVGVHGMEQGAEEERICPNSAANGMENTGDGSSQHSRRLMIDAHDNTGEEATQRIDLVQRLEGETWGQMNAISAFLMGEEDVENNRGRLVQPGAACSSGGAACNTNKIKGDALPTRKMVGQAFEEHKKTISSLLMRNEVSSIGIYGMGGVGKTTLGTHIHNQLLERPETPVYWITVSHNTSIPRLQTSLAGRIGLDLSKVDEELHRAVALKKELMKKQKWILILDDLWKAFDLQKLGVPDQVEEGCKLILTSRSAKVCQQMKTQHTIKVQPISEKEAWTLFIERLGHDIAFSSEVEGIALNVVRECAGLPLGIITIAASMRGVDEPHEWRNTLKKLKESKYKEMEDEVFRLLRFSYDQLNDLALQQCLLYCALYPEDHRIEREELIGYLIDEEIIEGMRSRQAAFDEGRTMLDKLEKVCLLERACYGDHSTTVKMHDLIRDMAHQILQTNSPVMVGGYNDKLPDVDMWKENLVRVSLKHCYFEEIPSSHSPRCPNLSTLLLCDNPYLQFIADSFFTQLHGLKVLDLSRTEIIELPDSVSELVSLTALLLKQCEYLIHVPSLEKLTALRRLDLSGTWELEKIPQDMQCLSNLRYLRMDGCGVKEFPTGILPKLFHLQLFMLEGKTNYDYVQVTVKGKEVGCLRELENLVCNFEGQSDFVEYLNSRDKTRSLSTYDIFVGPLDEDFYSEMKRGYGKSKTVWLSSLCNNGDGNFQVMFPNDIQKLIIFNCSCDVSSLIEHSIKLEVIHIKDCNSMESLISSSLFCPSPTSLPSYNGVFSTLKEFNCYGCSSMKKLFPLVLLPNLVNLVNITVIDCAKMEEIIGGRRSDEENSSNNTEFKLPKLRSLALSWLPKLKRICSAKLICDSLQQIEVSYCNSMESLVPSSWISLVNLEKITVRGCQKMEEIIGGRRSDEESSSNNTEFKLPKLRSLALEDLPELKSICSAKLTCDSLQQIEVWNCNSMESLVPSSWISLVNLEKITVRGGEKMEEIIGGRRSDEESSSTEFKLPKLRSLALFNLPELKSICSAKLTCDSLQQIEVWNCNSMEILVPSSWISLVNLEKITVSACKKMKEIIGGTRSDEESSSNNTEFKLPKLRSLALSWLPELKRICSAKLICDSLRMIEVYKCQKLKRMPLWKKDYTSKRMVGVSSGVGAS

Target ORF information:

RefSeq Version	XM_024591912.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024591912.1

ORF Insert Sequence:

ATGAGTCGAC CAAATGATCC TTTTTGGGAA TATGCTGAAA AGATGGATGA TGGTAGCATG 
ACGTGTACGT TTTGTGGGCA TTGTTTTTCC CAAGGTACTT CCGTTACGAG GATCAAATTG 
CATTTGGCAG GAGTGAAACG GCGTGGTGTT AAAATTTGTA AAAATGTTCC AGAAGAAGTT 
CGAGATGCAG CTTGTGATGC AGTTAATAAT AGCCCTCCAG AAAAGAAACT TAAATCTGTT 
GCGGGCTCAA GCAGTAACGA GGTCGCTAAT GCAATTTCAG CTTCAACGCA AGAACAGAAC 
AATGAAGTGA CCCATGTAGA GATGGCACAA CAAGGAGGAC CTTTTTTCAC TGGAGAACTT 
GCATGGGCAA ATGATTTGAT TGGAGAGGCT GAACTTGTGC AGCTGGAGAG AGGTAGTTCT 
CATGAGAGGC CATCAATTAA TCAAACAGAT GAGCCTCGAG GAGATTCATC CCGACCAACA 
GATGATCAAC TCTGTTCTCC ATCAGTAAAC AATGATGCCA ATATGAACGA TGTGCAGAAC 
ATGGTTGCAG TCAGGATAGA ACCAGTACCG GTTCAAGTGT TGGAACAAAG CAATGCAGAA 
GTTGACAGTT TGGCAGGGCA TGCTGGAAGG ATACAAGTGG GAGTTCATGG CATGGAACAA 
GGTGCGGAGG AAGAGAGAAT TTGTCCGAAC TCAGCGGCAA ATGGCATGGA AAACACTGGT 
GATGGATCTA GTCAGCATTC CAGGAGACTC ATGATTGATG CACATGATAA TACAGGGGAA 
GAAGCTACAC AAAGAATAGA TCTAGTGCAA CGATTAGAAG GGGAAACTTG GGGTCAGATG 
AATGCAATCT CTGCTTTTTT AATGGGGGAG GAGGACGTGG AGAACAATCG TGGAAGATTA 
GTGCAGCCTG GAGCAGCTTG CTCTTCTGGA GGGGCTGCAT GCAACACAAA TAAGATTAAA 
GGAGATGCAC TGCCAACGAG AAAAATGGTG GGTCAAGCAT TCGAAGAACA TAAGAAGACT 
ATCTCATCTT TGTTGATGCG CAATGAAGTT TCAAGTATTG GTATCTATGG GATGGGCGGA 
GTGGGAAAGA CCACATTGGG GACACATATA CATAATCAGC TTCTAGAAAG ACCAGAGACT 
CCTGTTTACT GGATTACTGT TTCACATAAT ACCAGCATTC CTAGATTGCA GACAAGTCTC 
GCAGGACGAA TTGGTTTAGA TCTTTCCAAG GTAGATGAGG AGCTGCATAG AGCGGTAGCG 
TTGAAAAAAG AATTAATGAA GAAACAGAAA TGGATTCTGA TTTTAGATGA TTTGTGGAAG 
GCTTTTGACC TGCAAAAGCT GGGAGTTCCT GACCAAGTTG AGGAGGGATG CAAGCTGATT 
CTTACATCTC GATCAGCAAA GGTTTGTCAA CAGATGAAAA CCCAACACAC GATAAAAGTG 
CAGCCTATTT CGGAGAAAGA AGCTTGGACT TTGTTTATTG AGAGACTTGG ACATGACATA 
GCATTTTCTT CAGAAGTGGA AGGAATTGCT TTGAATGTTG TAAGGGAATG TGCTGGTTTG 
CCATTGGGAA TTATTACAAT AGCAGCAAGC ATGAGAGGAG TGGATGAGCC ACATGAGTGG 
AGGAATACAT TGAAGAAATT GAAAGAATCA AAATATAAGG AAATGGAAGA TGAGGTATTC 
CGATTATTGA GGTTTAGTTA TGATCAGCTA AATGATTTAG CATTACAACA ATGTCTCTTG 
TACTGTGCAT TGTATCCTGA AGATCATAGG ATTGAAAGGG AGGAGCTGAT AGGTTATTTG 
ATCGACGAGG AAATAATTGA AGGAATGAGG AGCAGGCAAG CAGCATTTGA CGAGGGCCGC 
ACGATGCTTG ATAAACTTGA AAAAGTCTGT TTATTGGAAA GAGCTTGTTA TGGTGATCAT 
AGTACAACTG TCAAGATGCA TGACTTGATT AGGGACATGG CCCACCAAAT ACTCCAAACA 
AACTCTCCAG TCATGGTTGG GGGATATAAT GATAAATTAC CAGATGTGGA TATGTGGAAA 
GAGAATCTAG TGAGAGTCTC TCTGAAGCAT TGTTATTTCG AGGAAATTCC TTCTAGTCAT 
TCACCAAGAT GTCCCAATCT TTCAACTCTA TTGCTGTGTG ATAATCCTTA TTTGCAATTT 
ATTGCAGATT CTTTTTTCAC ACAATTGCAT GGGCTCAAGG TTCTTGATCT GTCTCGTACA 
GAAATCATAG AATTGCCTGA TTCTGTCTCT GAACTGGTGA GTCTCACTGC ATTATTGCTC 
AAACAATGTG AATATTTAAT ACATGTACCA TCATTAGAAA AGCTCACAGC ACTGAGGAGG 
CTAGATCTCT CTGGTACTTG GGAACTTGAA AAGATACCTC AAGACATGCA ATGTCTATCT 
AACCTGAGGT ATCTGAGGAT GGATGGATGT GGTGTAAAGG AGTTTCCTAC TGGGATATTA 
CCAAAACTCT TTCACCTACA ACTCTTCATG TTAGAGGGGA AGACTAATTA TGACTACGTT 
CAAGTAACAG TTAAGGGAAA GGAAGTAGGA TGCTTGAGGG AGTTGGAAAA TTTGGTATGC 
AATTTTGAAG GTCAATCTGA CTTTGTGGAG TATCTCAATT CTCGGGATAA GACCCGATCA 
CTAAGTACAT ACGATATTTT TGTAGGACCA CTGGATGAAG ATTTTTATAG TGAAATGAAG 
CGTGGTTATG GCAAAAGTAA AACAGTTTGG TTGAGTAGTT TGTGTAACAA TGGAGATGGA 
AATTTTCAAG TAATGTTCCC AAATGACATT CAAAAACTGA TTATTTTTAA TTGTTCATGT 
GATGTTTCCT CTTTAATAGA GCATTCAATT AAACTGGAGG TCATCCACAT TAAGGATTGC 
AATAGCATGG AGAGCTTGAT TTCATCTTCT TTGTTCTGCC CTTCTCCAAC ATCATTGCCA 
TCTTATAATG GTGTATTTTC TACTCTTAAA GAGTTTAATT GTTATGGATG TAGTAGTATG 
AAGAAGTTGT TCCCTCTTGT CTTGCTGCCA AACCTCGTAA ACCTAGTAAA TATTACAGTT 
ATAGATTGTG CAAAAATGGA GGAGATAATA GGTGGAAGAA GATCAGATGA AGAAAACAGC 
AGCAACAACA CCGAATTCAA GCTCCCAAAG TTAAGATCTC TGGCATTGAG TTGGTTACCA 
AAACTGAAAA GAATTTGTAG TGCAAAACTG ATTTGCGATT CTCTCCAACA AATTGAAGTA 
AGTTACTGCA ATAGCATGGA GAGCTTGGTT CCATCTTCTT GGATTTCCCT CGTAAACCTA 
GAAAAGATTA CAGTTAGAGG ATGTCAGAAG ATGGAGGAGA TAATAGGTGG AAGAAGATCA 
GATGAAGAAA GCAGCAGCAA CAACACCGAA TTCAAACTCC CAAAGTTAAG ATCTCTGGCA 
TTGGAAGATT TACCAGAACT GAAAAGCATT TGTAGTGCAA AGCTGACGTG CGATTCTCTC 
CAACAAATTG AAGTATGGAA TTGCAACAGC ATGGAGAGCT TGGTTCCATC TTCTTGGATT 
TCCCTCGTAA ACCTAGAAAA GATTACAGTT AGAGGAGGTG AGAAAATGGA GGAGATAATA 
GGTGGAAGAA GATCAGATGA AGAAAGCAGC AGCACCGAAT TCAAACTCCC AAAGTTAAGA 
TCTCTGGCAT TGTTTAATTT ACCAGAACTG AAAAGCATTT GTAGTGCAAA GCTGACGTGC 
GATTCTCTCC AACAAATTGA AGTATGGAAT TGCAACAGCA TGGAGATCTT GGTTCCATCT 
TCTTGGATTT CCCTTGTAAA CCTAGAAAAG ATTACAGTTA GTGCATGTAA GAAGATGAAG 
GAGATAATAG GTGGAACAAG ATCAGATGAA GAAAGTAGCA GTAACAACAC CGAATTCAAA 
CTCCCAAAGT TAAGATCTCT GGCATTGAGT TGGTTACCAG AACTGAAAAG AATTTGTAGT 
GCAAAACTGA TTTGCGATTC TCTCAGAATG ATTGAAGTTT ATAAATGTCA GAAGCTGAAG 
AGGATGCCAT TGTGGAAGAA GGATTATACA TCCAAGAGAA TGGTGGGAGT CAGTAGTGGT 
GTGGGAGCAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18107958 ( XM_024591917.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024447685.1 Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OPa418670
Clone ID Related Accession (Same CDS sequence)	XM_024591920.1 , XM_024591911.1 , XM_024591912.1 , XM_024591917.1 , XM_024591913.1 , XM_024591916.1 , XM_024591918.1 , XM_024591919.1 , XM_024591910.1 , XM_024591914.1
Accession Version	XM_024591917.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	probable disease resistance protein At4g27220 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020167869.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCGAC CAAATGATCC TTTTTGGGAA TATGCTGAAA AGATGGATGA TGGTAGCATG 
ACGTGTACGT TTTGTGGGCA TTGTTTTTCC CAAGGTACTT CCGTTACGAG GATCAAATTG 
CATTTGGCAG GAGTGAAACG GCGTGGTGTT AAAATTTGTA AAAATGTTCC AGAAGAAGTT 
CGAGATGCAG CTTGTGATGC AGTTAATAAT AGCCCTCCAG AAAAGAAACT TAAATCTGTT 
GCGGGCTCAA GCAGTAACGA GGTCGCTAAT GCAATTTCAG CTTCAACGCA AGAACAGAAC 
AATGAAGTGA CCCATGTAGA GATGGCACAA CAAGGAGGAC CTTTTTTCAC TGGAGAACTT 
GCATGGGCAA ATGATTTGAT TGGAGAGGCT GAACTTGTGC AGCTGGAGAG AGGTAGTTCT 
CATGAGAGGC CATCAATTAA TCAAACAGAT GAGCCTCGAG GAGATTCATC CCGACCAACA 
GATGATCAAC TCTGTTCTCC ATCAGTAAAC AATGATGCCA ATATGAACGA TGTGCAGAAC 
ATGGTTGCAG TCAGGATAGA ACCAGTACCG GTTCAAGTGT TGGAACAAAG CAATGCAGAA 
GTTGACAGTT TGGCAGGGCA TGCTGGAAGG ATACAAGTGG GAGTTCATGG CATGGAACAA 
GGTGCGGAGG AAGAGAGAAT TTGTCCGAAC TCAGCGGCAA ATGGCATGGA AAACACTGGT 
GATGGATCTA GTCAGCATTC CAGGAGACTC ATGATTGATG CACATGATAA TACAGGGGAA 
GAAGCTACAC AAAGAATAGA TCTAGTGCAA CGATTAGAAG GGGAAACTTG GGGTCAGATG 
AATGCAATCT CTGCTTTTTT AATGGGGGAG GAGGACGTGG AGAACAATCG TGGAAGATTA 
GTGCAGCCTG GAGCAGCTTG CTCTTCTGGA GGGGCTGCAT GCAACACAAA TAAGATTAAA 
GGAGATGCAC TGCCAACGAG AAAAATGGTG GGTCAAGCAT TCGAAGAACA TAAGAAGACT 
ATCTCATCTT TGTTGATGCG CAATGAAGTT TCAAGTATTG GTATCTATGG GATGGGCGGA 
GTGGGAAAGA CCACATTGGG GACACATATA CATAATCAGC TTCTAGAAAG ACCAGAGACT 
CCTGTTTACT GGATTACTGT TTCACATAAT ACCAGCATTC CTAGATTGCA GACAAGTCTC 
GCAGGACGAA TTGGTTTAGA TCTTTCCAAG GTAGATGAGG AGCTGCATAG AGCGGTAGCG 
TTGAAAAAAG AATTAATGAA GAAACAGAAA TGGATTCTGA TTTTAGATGA TTTGTGGAAG 
GCTTTTGACC TGCAAAAGCT GGGAGTTCCT GACCAAGTTG AGGAGGGATG CAAGCTGATT 
CTTACATCTC GATCAGCAAA GGTTTGTCAA CAGATGAAAA CCCAACACAC GATAAAAGTG 
CAGCCTATTT CGGAGAAAGA AGCTTGGACT TTGTTTATTG AGAGACTTGG ACATGACATA 
GCATTTTCTT CAGAAGTGGA AGGAATTGCT TTGAATGTTG TAAGGGAATG TGCTGGTTTG 
CCATTGGGAA TTATTACAAT AGCAGCAAGC ATGAGAGGAG TGGATGAGCC ACATGAGTGG 
AGGAATACAT TGAAGAAATT GAAAGAATCA AAATATAAGG AAATGGAAGA TGAGGTATTC 
CGATTATTGA GGTTTAGTTA TGATCAGCTA AATGATTTAG CATTACAACA ATGTCTCTTG 
TACTGTGCAT TGTATCCTGA AGATCATAGG ATTGAAAGGG AGGAGCTGAT AGGTTATTTG 
ATCGACGAGG AAATAATTGA AGGAATGAGG AGCAGGCAAG CAGCATTTGA CGAGGGCCGC 
ACGATGCTTG ATAAACTTGA AAAAGTCTGT TTATTGGAAA GAGCTTGTTA TGGTGATCAT 
AGTACAACTG TCAAGATGCA TGACTTGATT AGGGACATGG CCCACCAAAT ACTCCAAACA 
AACTCTCCAG TCATGGTTGG GGGATATAAT GATAAATTAC CAGATGTGGA TATGTGGAAA 
GAGAATCTAG TGAGAGTCTC TCTGAAGCAT TGTTATTTCG AGGAAATTCC TTCTAGTCAT 
TCACCAAGAT GTCCCAATCT TTCAACTCTA TTGCTGTGTG ATAATCCTTA TTTGCAATTT 
ATTGCAGATT CTTTTTTCAC ACAATTGCAT GGGCTCAAGG TTCTTGATCT GTCTCGTACA 
GAAATCATAG AATTGCCTGA TTCTGTCTCT GAACTGGTGA GTCTCACTGC ATTATTGCTC 
AAACAATGTG AATATTTAAT ACATGTACCA TCATTAGAAA AGCTCACAGC ACTGAGGAGG 
CTAGATCTCT CTGGTACTTG GGAACTTGAA AAGATACCTC AAGACATGCA ATGTCTATCT 
AACCTGAGGT ATCTGAGGAT GGATGGATGT GGTGTAAAGG AGTTTCCTAC TGGGATATTA 
CCAAAACTCT TTCACCTACA ACTCTTCATG TTAGAGGGGA AGACTAATTA TGACTACGTT 
CAAGTAACAG TTAAGGGAAA GGAAGTAGGA TGCTTGAGGG AGTTGGAAAA TTTGGTATGC 
AATTTTGAAG GTCAATCTGA CTTTGTGGAG TATCTCAATT CTCGGGATAA GACCCGATCA 
CTAAGTACAT ACGATATTTT TGTAGGACCA CTGGATGAAG ATTTTTATAG TGAAATGAAG 
CGTGGTTATG GCAAAAGTAA AACAGTTTGG TTGAGTAGTT TGTGTAACAA TGGAGATGGA 
AATTTTCAAG TAATGTTCCC AAATGACATT CAAAAACTGA TTATTTTTAA TTGTTCATGT 
GATGTTTCCT CTTTAATAGA GCATTCAATT AAACTGGAGG TCATCCACAT TAAGGATTGC 
AATAGCATGG AGAGCTTGAT TTCATCTTCT TTGTTCTGCC CTTCTCCAAC ATCATTGCCA 
TCTTATAATG GTGTATTTTC TACTCTTAAA GAGTTTAATT GTTATGGATG TAGTAGTATG 
AAGAAGTTGT TCCCTCTTGT CTTGCTGCCA AACCTCGTAA ACCTAGTAAA TATTACAGTT 
ATAGATTGTG CAAAAATGGA GGAGATAATA GGTGGAAGAA GATCAGATGA AGAAAACAGC 
AGCAACAACA CCGAATTCAA GCTCCCAAAG TTAAGATCTC TGGCATTGAG TTGGTTACCA 
AAACTGAAAA GAATTTGTAG TGCAAAACTG ATTTGCGATT CTCTCCAACA AATTGAAGTA 
AGTTACTGCA ATAGCATGGA GAGCTTGGTT CCATCTTCTT GGATTTCCCT CGTAAACCTA 
GAAAAGATTA CAGTTAGAGG ATGTCAGAAG ATGGAGGAGA TAATAGGTGG AAGAAGATCA 
GATGAAGAAA GCAGCAGCAA CAACACCGAA TTCAAACTCC CAAAGTTAAG ATCTCTGGCA 
TTGGAAGATT TACCAGAACT GAAAAGCATT TGTAGTGCAA AGCTGACGTG CGATTCTCTC 
CAACAAATTG AAGTATGGAA TTGCAACAGC ATGGAGAGCT TGGTTCCATC TTCTTGGATT 
TCCCTCGTAA ACCTAGAAAA GATTACAGTT AGAGGAGGTG AGAAAATGGA GGAGATAATA 
GGTGGAAGAA GATCAGATGA AGAAAGCAGC AGCACCGAAT TCAAACTCCC AAAGTTAAGA 
TCTCTGGCAT TGTTTAATTT ACCAGAACTG AAAAGCATTT GTAGTGCAAA GCTGACGTGC 
GATTCTCTCC AACAAATTGA AGTATGGAAT TGCAACAGCA TGGAGATCTT GGTTCCATCT 
TCTTGGATTT CCCTTGTAAA CCTAGAAAAG ATTACAGTTA GTGCATGTAA GAAGATGAAG 
GAGATAATAG GTGGAACAAG ATCAGATGAA GAAAGTAGCA GTAACAACAC CGAATTCAAA 
CTCCCAAAGT TAAGATCTCT GGCATTGAGT TGGTTACCAG AACTGAAAAG AATTTGTAGT 
GCAAAACTGA TTTGCGATTC TCTCAGAATG ATTGAAGTTT ATAAATGTCA GAAGCTGAAG 
AGGATGCCAT TGTGGAAGAA GGATTATACA TCCAAGAGAA TGGTGGGAGT CAGTAGTGGT 
GTGGGAGCAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024447685.1
CDS	893..4987
Translation	MSRPNDPFWEYAEKMDDGSMTCTFCGHCFSQGTSVTRIKLHLAGVKRRGVKICKNVPEEVRDAACDAVNNSPPEKKLKSVAGSSSNEVANAISASTQEQNNEVTHVEMAQQGGPFFTGELAWANDLIGEAELVQLERGSSHERPSINQTDEPRGDSSRPTDDQLCSPSVNNDANMNDVQNMVAVRIEPVPVQVLEQSNAEVDSLAGHAGRIQVGVHGMEQGAEEERICPNSAANGMENTGDGSSQHSRRLMIDAHDNTGEEATQRIDLVQRLEGETWGQMNAISAFLMGEEDVENNRGRLVQPGAACSSGGAACNTNKIKGDALPTRKMVGQAFEEHKKTISSLLMRNEVSSIGIYGMGGVGKTTLGTHIHNQLLERPETPVYWITVSHNTSIPRLQTSLAGRIGLDLSKVDEELHRAVALKKELMKKQKWILILDDLWKAFDLQKLGVPDQVEEGCKLILTSRSAKVCQQMKTQHTIKVQPISEKEAWTLFIERLGHDIAFSSEVEGIALNVVRECAGLPLGIITIAASMRGVDEPHEWRNTLKKLKESKYKEMEDEVFRLLRFSYDQLNDLALQQCLLYCALYPEDHRIEREELIGYLIDEEIIEGMRSRQAAFDEGRTMLDKLEKVCLLERACYGDHSTTVKMHDLIRDMAHQILQTNSPVMVGGYNDKLPDVDMWKENLVRVSLKHCYFEEIPSSHSPRCPNLSTLLLCDNPYLQFIADSFFTQLHGLKVLDLSRTEIIELPDSVSELVSLTALLLKQCEYLIHVPSLEKLTALRRLDLSGTWELEKIPQDMQCLSNLRYLRMDGCGVKEFPTGILPKLFHLQLFMLEGKTNYDYVQVTVKGKEVGCLRELENLVCNFEGQSDFVEYLNSRDKTRSLSTYDIFVGPLDEDFYSEMKRGYGKSKTVWLSSLCNNGDGNFQVMFPNDIQKLIIFNCSCDVSSLIEHSIKLEVIHIKDCNSMESLISSSLFCPSPTSLPSYNGVFSTLKEFNCYGCSSMKKLFPLVLLPNLVNLVNITVIDCAKMEEIIGGRRSDEENSSNNTEFKLPKLRSLALSWLPKLKRICSAKLICDSLQQIEVSYCNSMESLVPSSWISLVNLEKITVRGCQKMEEIIGGRRSDEESSSNNTEFKLPKLRSLALEDLPELKSICSAKLTCDSLQQIEVWNCNSMESLVPSSWISLVNLEKITVRGGEKMEEIIGGRRSDEESSSTEFKLPKLRSLALFNLPELKSICSAKLTCDSLQQIEVWNCNSMEILVPSSWISLVNLEKITVSACKKMKEIIGGTRSDEESSSNNTEFKLPKLRSLALSWLPELKRICSAKLICDSLRMIEVYKCQKLKRMPLWKKDYTSKRMVGVSSGVGAS

Target ORF information:

RefSeq Version	XM_024591917.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024591917.1

ORF Insert Sequence:

ATGAGTCGAC CAAATGATCC TTTTTGGGAA TATGCTGAAA AGATGGATGA TGGTAGCATG 
ACGTGTACGT TTTGTGGGCA TTGTTTTTCC CAAGGTACTT CCGTTACGAG GATCAAATTG 
CATTTGGCAG GAGTGAAACG GCGTGGTGTT AAAATTTGTA AAAATGTTCC AGAAGAAGTT 
CGAGATGCAG CTTGTGATGC AGTTAATAAT AGCCCTCCAG AAAAGAAACT TAAATCTGTT 
GCGGGCTCAA GCAGTAACGA GGTCGCTAAT GCAATTTCAG CTTCAACGCA AGAACAGAAC 
AATGAAGTGA CCCATGTAGA GATGGCACAA CAAGGAGGAC CTTTTTTCAC TGGAGAACTT 
GCATGGGCAA ATGATTTGAT TGGAGAGGCT GAACTTGTGC AGCTGGAGAG AGGTAGTTCT 
CATGAGAGGC CATCAATTAA TCAAACAGAT GAGCCTCGAG GAGATTCATC CCGACCAACA 
GATGATCAAC TCTGTTCTCC ATCAGTAAAC AATGATGCCA ATATGAACGA TGTGCAGAAC 
ATGGTTGCAG TCAGGATAGA ACCAGTACCG GTTCAAGTGT TGGAACAAAG CAATGCAGAA 
GTTGACAGTT TGGCAGGGCA TGCTGGAAGG ATACAAGTGG GAGTTCATGG CATGGAACAA 
GGTGCGGAGG AAGAGAGAAT TTGTCCGAAC TCAGCGGCAA ATGGCATGGA AAACACTGGT 
GATGGATCTA GTCAGCATTC CAGGAGACTC ATGATTGATG CACATGATAA TACAGGGGAA 
GAAGCTACAC AAAGAATAGA TCTAGTGCAA CGATTAGAAG GGGAAACTTG GGGTCAGATG 
AATGCAATCT CTGCTTTTTT AATGGGGGAG GAGGACGTGG AGAACAATCG TGGAAGATTA 
GTGCAGCCTG GAGCAGCTTG CTCTTCTGGA GGGGCTGCAT GCAACACAAA TAAGATTAAA 
GGAGATGCAC TGCCAACGAG AAAAATGGTG GGTCAAGCAT TCGAAGAACA TAAGAAGACT 
ATCTCATCTT TGTTGATGCG CAATGAAGTT TCAAGTATTG GTATCTATGG GATGGGCGGA 
GTGGGAAAGA CCACATTGGG GACACATATA CATAATCAGC TTCTAGAAAG ACCAGAGACT 
CCTGTTTACT GGATTACTGT TTCACATAAT ACCAGCATTC CTAGATTGCA GACAAGTCTC 
GCAGGACGAA TTGGTTTAGA TCTTTCCAAG GTAGATGAGG AGCTGCATAG AGCGGTAGCG 
TTGAAAAAAG AATTAATGAA GAAACAGAAA TGGATTCTGA TTTTAGATGA TTTGTGGAAG 
GCTTTTGACC TGCAAAAGCT GGGAGTTCCT GACCAAGTTG AGGAGGGATG CAAGCTGATT 
CTTACATCTC GATCAGCAAA GGTTTGTCAA CAGATGAAAA CCCAACACAC GATAAAAGTG 
CAGCCTATTT CGGAGAAAGA AGCTTGGACT TTGTTTATTG AGAGACTTGG ACATGACATA 
GCATTTTCTT CAGAAGTGGA AGGAATTGCT TTGAATGTTG TAAGGGAATG TGCTGGTTTG 
CCATTGGGAA TTATTACAAT AGCAGCAAGC ATGAGAGGAG TGGATGAGCC ACATGAGTGG 
AGGAATACAT TGAAGAAATT GAAAGAATCA AAATATAAGG AAATGGAAGA TGAGGTATTC 
CGATTATTGA GGTTTAGTTA TGATCAGCTA AATGATTTAG CATTACAACA ATGTCTCTTG 
TACTGTGCAT TGTATCCTGA AGATCATAGG ATTGAAAGGG AGGAGCTGAT AGGTTATTTG 
ATCGACGAGG AAATAATTGA AGGAATGAGG AGCAGGCAAG CAGCATTTGA CGAGGGCCGC 
ACGATGCTTG ATAAACTTGA AAAAGTCTGT TTATTGGAAA GAGCTTGTTA TGGTGATCAT 
AGTACAACTG TCAAGATGCA TGACTTGATT AGGGACATGG CCCACCAAAT ACTCCAAACA 
AACTCTCCAG TCATGGTTGG GGGATATAAT GATAAATTAC CAGATGTGGA TATGTGGAAA 
GAGAATCTAG TGAGAGTCTC TCTGAAGCAT TGTTATTTCG AGGAAATTCC TTCTAGTCAT 
TCACCAAGAT GTCCCAATCT TTCAACTCTA TTGCTGTGTG ATAATCCTTA TTTGCAATTT 
ATTGCAGATT CTTTTTTCAC ACAATTGCAT GGGCTCAAGG TTCTTGATCT GTCTCGTACA 
GAAATCATAG AATTGCCTGA TTCTGTCTCT GAACTGGTGA GTCTCACTGC ATTATTGCTC 
AAACAATGTG AATATTTAAT ACATGTACCA TCATTAGAAA AGCTCACAGC ACTGAGGAGG 
CTAGATCTCT CTGGTACTTG GGAACTTGAA AAGATACCTC AAGACATGCA ATGTCTATCT 
AACCTGAGGT ATCTGAGGAT GGATGGATGT GGTGTAAAGG AGTTTCCTAC TGGGATATTA 
CCAAAACTCT TTCACCTACA ACTCTTCATG TTAGAGGGGA AGACTAATTA TGACTACGTT 
CAAGTAACAG TTAAGGGAAA GGAAGTAGGA TGCTTGAGGG AGTTGGAAAA TTTGGTATGC 
AATTTTGAAG GTCAATCTGA CTTTGTGGAG TATCTCAATT CTCGGGATAA GACCCGATCA 
CTAAGTACAT ACGATATTTT TGTAGGACCA CTGGATGAAG ATTTTTATAG TGAAATGAAG 
CGTGGTTATG GCAAAAGTAA AACAGTTTGG TTGAGTAGTT TGTGTAACAA TGGAGATGGA 
AATTTTCAAG TAATGTTCCC AAATGACATT CAAAAACTGA TTATTTTTAA TTGTTCATGT 
GATGTTTCCT CTTTAATAGA GCATTCAATT AAACTGGAGG TCATCCACAT TAAGGATTGC 
AATAGCATGG AGAGCTTGAT TTCATCTTCT TTGTTCTGCC CTTCTCCAAC ATCATTGCCA 
TCTTATAATG GTGTATTTTC TACTCTTAAA GAGTTTAATT GTTATGGATG TAGTAGTATG 
AAGAAGTTGT TCCCTCTTGT CTTGCTGCCA AACCTCGTAA ACCTAGTAAA TATTACAGTT 
ATAGATTGTG CAAAAATGGA GGAGATAATA GGTGGAAGAA GATCAGATGA AGAAAACAGC 
AGCAACAACA CCGAATTCAA GCTCCCAAAG TTAAGATCTC TGGCATTGAG TTGGTTACCA 
AAACTGAAAA GAATTTGTAG TGCAAAACTG ATTTGCGATT CTCTCCAACA AATTGAAGTA 
AGTTACTGCA ATAGCATGGA GAGCTTGGTT CCATCTTCTT GGATTTCCCT CGTAAACCTA 
GAAAAGATTA CAGTTAGAGG ATGTCAGAAG ATGGAGGAGA TAATAGGTGG AAGAAGATCA 
GATGAAGAAA GCAGCAGCAA CAACACCGAA TTCAAACTCC CAAAGTTAAG ATCTCTGGCA 
TTGGAAGATT TACCAGAACT GAAAAGCATT TGTAGTGCAA AGCTGACGTG CGATTCTCTC 
CAACAAATTG AAGTATGGAA TTGCAACAGC ATGGAGAGCT TGGTTCCATC TTCTTGGATT 
TCCCTCGTAA ACCTAGAAAA GATTACAGTT AGAGGAGGTG AGAAAATGGA GGAGATAATA 
GGTGGAAGAA GATCAGATGA AGAAAGCAGC AGCACCGAAT TCAAACTCCC AAAGTTAAGA 
TCTCTGGCAT TGTTTAATTT ACCAGAACTG AAAAGCATTT GTAGTGCAAA GCTGACGTGC 
GATTCTCTCC AACAAATTGA AGTATGGAAT TGCAACAGCA TGGAGATCTT GGTTCCATCT 
TCTTGGATTT CCCTTGTAAA CCTAGAAAAG ATTACAGTTA GTGCATGTAA GAAGATGAAG 
GAGATAATAG GTGGAACAAG ATCAGATGAA GAAAGTAGCA GTAACAACAC CGAATTCAAA 
CTCCCAAAGT TAAGATCTCT GGCATTGAGT TGGTTACCAG AACTGAAAAG AATTTGTAGT 
GCAAAACTGA TTTGCGATTC TCTCAGAATG ATTGAAGTTT ATAAATGTCA GAAGCTGAAG 
AGGATGCCAT TGTGGAAGAA GGATTATACA TCCAAGAGAA TGGTGGGAGT CAGTAGTGGT 
GTGGGAGCAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18107958 ( XM_024591913.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024447681.1 Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X4, mRNA.

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CloneID	OPa418670
Clone ID Related Accession (Same CDS sequence)	XM_024591920.1 , XM_024591911.1 , XM_024591912.1 , XM_024591917.1 , XM_024591913.1 , XM_024591916.1 , XM_024591918.1 , XM_024591919.1 , XM_024591910.1 , XM_024591914.1
Accession Version	XM_024591913.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	probable disease resistance protein At4g27220 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020167869.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCGAC CAAATGATCC TTTTTGGGAA TATGCTGAAA AGATGGATGA TGGTAGCATG 
ACGTGTACGT TTTGTGGGCA TTGTTTTTCC CAAGGTACTT CCGTTACGAG GATCAAATTG 
CATTTGGCAG GAGTGAAACG GCGTGGTGTT AAAATTTGTA AAAATGTTCC AGAAGAAGTT 
CGAGATGCAG CTTGTGATGC AGTTAATAAT AGCCCTCCAG AAAAGAAACT TAAATCTGTT 
GCGGGCTCAA GCAGTAACGA GGTCGCTAAT GCAATTTCAG CTTCAACGCA AGAACAGAAC 
AATGAAGTGA CCCATGTAGA GATGGCACAA CAAGGAGGAC CTTTTTTCAC TGGAGAACTT 
GCATGGGCAA ATGATTTGAT TGGAGAGGCT GAACTTGTGC AGCTGGAGAG AGGTAGTTCT 
CATGAGAGGC CATCAATTAA TCAAACAGAT GAGCCTCGAG GAGATTCATC CCGACCAACA 
GATGATCAAC TCTGTTCTCC ATCAGTAAAC AATGATGCCA ATATGAACGA TGTGCAGAAC 
ATGGTTGCAG TCAGGATAGA ACCAGTACCG GTTCAAGTGT TGGAACAAAG CAATGCAGAA 
GTTGACAGTT TGGCAGGGCA TGCTGGAAGG ATACAAGTGG GAGTTCATGG CATGGAACAA 
GGTGCGGAGG AAGAGAGAAT TTGTCCGAAC TCAGCGGCAA ATGGCATGGA AAACACTGGT 
GATGGATCTA GTCAGCATTC CAGGAGACTC ATGATTGATG CACATGATAA TACAGGGGAA 
GAAGCTACAC AAAGAATAGA TCTAGTGCAA CGATTAGAAG GGGAAACTTG GGGTCAGATG 
AATGCAATCT CTGCTTTTTT AATGGGGGAG GAGGACGTGG AGAACAATCG TGGAAGATTA 
GTGCAGCCTG GAGCAGCTTG CTCTTCTGGA GGGGCTGCAT GCAACACAAA TAAGATTAAA 
GGAGATGCAC TGCCAACGAG AAAAATGGTG GGTCAAGCAT TCGAAGAACA TAAGAAGACT 
ATCTCATCTT TGTTGATGCG CAATGAAGTT TCAAGTATTG GTATCTATGG GATGGGCGGA 
GTGGGAAAGA CCACATTGGG GACACATATA CATAATCAGC TTCTAGAAAG ACCAGAGACT 
CCTGTTTACT GGATTACTGT TTCACATAAT ACCAGCATTC CTAGATTGCA GACAAGTCTC 
GCAGGACGAA TTGGTTTAGA TCTTTCCAAG GTAGATGAGG AGCTGCATAG AGCGGTAGCG 
TTGAAAAAAG AATTAATGAA GAAACAGAAA TGGATTCTGA TTTTAGATGA TTTGTGGAAG 
GCTTTTGACC TGCAAAAGCT GGGAGTTCCT GACCAAGTTG AGGAGGGATG CAAGCTGATT 
CTTACATCTC GATCAGCAAA GGTTTGTCAA CAGATGAAAA CCCAACACAC GATAAAAGTG 
CAGCCTATTT CGGAGAAAGA AGCTTGGACT TTGTTTATTG AGAGACTTGG ACATGACATA 
GCATTTTCTT CAGAAGTGGA AGGAATTGCT TTGAATGTTG TAAGGGAATG TGCTGGTTTG 
CCATTGGGAA TTATTACAAT AGCAGCAAGC ATGAGAGGAG TGGATGAGCC ACATGAGTGG 
AGGAATACAT TGAAGAAATT GAAAGAATCA AAATATAAGG AAATGGAAGA TGAGGTATTC 
CGATTATTGA GGTTTAGTTA TGATCAGCTA AATGATTTAG CATTACAACA ATGTCTCTTG 
TACTGTGCAT TGTATCCTGA AGATCATAGG ATTGAAAGGG AGGAGCTGAT AGGTTATTTG 
ATCGACGAGG AAATAATTGA AGGAATGAGG AGCAGGCAAG CAGCATTTGA CGAGGGCCGC 
ACGATGCTTG ATAAACTTGA AAAAGTCTGT TTATTGGAAA GAGCTTGTTA TGGTGATCAT 
AGTACAACTG TCAAGATGCA TGACTTGATT AGGGACATGG CCCACCAAAT ACTCCAAACA 
AACTCTCCAG TCATGGTTGG GGGATATAAT GATAAATTAC CAGATGTGGA TATGTGGAAA 
GAGAATCTAG TGAGAGTCTC TCTGAAGCAT TGTTATTTCG AGGAAATTCC TTCTAGTCAT 
TCACCAAGAT GTCCCAATCT TTCAACTCTA TTGCTGTGTG ATAATCCTTA TTTGCAATTT 
ATTGCAGATT CTTTTTTCAC ACAATTGCAT GGGCTCAAGG TTCTTGATCT GTCTCGTACA 
GAAATCATAG AATTGCCTGA TTCTGTCTCT GAACTGGTGA GTCTCACTGC ATTATTGCTC 
AAACAATGTG AATATTTAAT ACATGTACCA TCATTAGAAA AGCTCACAGC ACTGAGGAGG 
CTAGATCTCT CTGGTACTTG GGAACTTGAA AAGATACCTC AAGACATGCA ATGTCTATCT 
AACCTGAGGT ATCTGAGGAT GGATGGATGT GGTGTAAAGG AGTTTCCTAC TGGGATATTA 
CCAAAACTCT TTCACCTACA ACTCTTCATG TTAGAGGGGA AGACTAATTA TGACTACGTT 
CAAGTAACAG TTAAGGGAAA GGAAGTAGGA TGCTTGAGGG AGTTGGAAAA TTTGGTATGC 
AATTTTGAAG GTCAATCTGA CTTTGTGGAG TATCTCAATT CTCGGGATAA GACCCGATCA 
CTAAGTACAT ACGATATTTT TGTAGGACCA CTGGATGAAG ATTTTTATAG TGAAATGAAG 
CGTGGTTATG GCAAAAGTAA AACAGTTTGG TTGAGTAGTT TGTGTAACAA TGGAGATGGA 
AATTTTCAAG TAATGTTCCC AAATGACATT CAAAAACTGA TTATTTTTAA TTGTTCATGT 
GATGTTTCCT CTTTAATAGA GCATTCAATT AAACTGGAGG TCATCCACAT TAAGGATTGC 
AATAGCATGG AGAGCTTGAT TTCATCTTCT TTGTTCTGCC CTTCTCCAAC ATCATTGCCA 
TCTTATAATG GTGTATTTTC TACTCTTAAA GAGTTTAATT GTTATGGATG TAGTAGTATG 
AAGAAGTTGT TCCCTCTTGT CTTGCTGCCA AACCTCGTAA ACCTAGTAAA TATTACAGTT 
ATAGATTGTG CAAAAATGGA GGAGATAATA GGTGGAAGAA GATCAGATGA AGAAAACAGC 
AGCAACAACA CCGAATTCAA GCTCCCAAAG TTAAGATCTC TGGCATTGAG TTGGTTACCA 
AAACTGAAAA GAATTTGTAG TGCAAAACTG ATTTGCGATT CTCTCCAACA AATTGAAGTA 
AGTTACTGCA ATAGCATGGA GAGCTTGGTT CCATCTTCTT GGATTTCCCT CGTAAACCTA 
GAAAAGATTA CAGTTAGAGG ATGTCAGAAG ATGGAGGAGA TAATAGGTGG AAGAAGATCA 
GATGAAGAAA GCAGCAGCAA CAACACCGAA TTCAAACTCC CAAAGTTAAG ATCTCTGGCA 
TTGGAAGATT TACCAGAACT GAAAAGCATT TGTAGTGCAA AGCTGACGTG CGATTCTCTC 
CAACAAATTG AAGTATGGAA TTGCAACAGC ATGGAGAGCT TGGTTCCATC TTCTTGGATT 
TCCCTCGTAA ACCTAGAAAA GATTACAGTT AGAGGAGGTG AGAAAATGGA GGAGATAATA 
GGTGGAAGAA GATCAGATGA AGAAAGCAGC AGCACCGAAT TCAAACTCCC AAAGTTAAGA 
TCTCTGGCAT TGTTTAATTT ACCAGAACTG AAAAGCATTT GTAGTGCAAA GCTGACGTGC 
GATTCTCTCC AACAAATTGA AGTATGGAAT TGCAACAGCA TGGAGATCTT GGTTCCATCT 
TCTTGGATTT CCCTTGTAAA CCTAGAAAAG ATTACAGTTA GTGCATGTAA GAAGATGAAG 
GAGATAATAG GTGGAACAAG ATCAGATGAA GAAAGTAGCA GTAACAACAC CGAATTCAAA 
CTCCCAAAGT TAAGATCTCT GGCATTGAGT TGGTTACCAG AACTGAAAAG AATTTGTAGT 
GCAAAACTGA TTTGCGATTC TCTCAGAATG ATTGAAGTTT ATAAATGTCA GAAGCTGAAG 
AGGATGCCAT TGTGGAAGAA GGATTATACA TCCAAGAGAA TGGTGGGAGT CAGTAGTGGT 
GTGGGAGCAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024447681.1
CDS	901..4995
Translation	MSRPNDPFWEYAEKMDDGSMTCTFCGHCFSQGTSVTRIKLHLAGVKRRGVKICKNVPEEVRDAACDAVNNSPPEKKLKSVAGSSSNEVANAISASTQEQNNEVTHVEMAQQGGPFFTGELAWANDLIGEAELVQLERGSSHERPSINQTDEPRGDSSRPTDDQLCSPSVNNDANMNDVQNMVAVRIEPVPVQVLEQSNAEVDSLAGHAGRIQVGVHGMEQGAEEERICPNSAANGMENTGDGSSQHSRRLMIDAHDNTGEEATQRIDLVQRLEGETWGQMNAISAFLMGEEDVENNRGRLVQPGAACSSGGAACNTNKIKGDALPTRKMVGQAFEEHKKTISSLLMRNEVSSIGIYGMGGVGKTTLGTHIHNQLLERPETPVYWITVSHNTSIPRLQTSLAGRIGLDLSKVDEELHRAVALKKELMKKQKWILILDDLWKAFDLQKLGVPDQVEEGCKLILTSRSAKVCQQMKTQHTIKVQPISEKEAWTLFIERLGHDIAFSSEVEGIALNVVRECAGLPLGIITIAASMRGVDEPHEWRNTLKKLKESKYKEMEDEVFRLLRFSYDQLNDLALQQCLLYCALYPEDHRIEREELIGYLIDEEIIEGMRSRQAAFDEGRTMLDKLEKVCLLERACYGDHSTTVKMHDLIRDMAHQILQTNSPVMVGGYNDKLPDVDMWKENLVRVSLKHCYFEEIPSSHSPRCPNLSTLLLCDNPYLQFIADSFFTQLHGLKVLDLSRTEIIELPDSVSELVSLTALLLKQCEYLIHVPSLEKLTALRRLDLSGTWELEKIPQDMQCLSNLRYLRMDGCGVKEFPTGILPKLFHLQLFMLEGKTNYDYVQVTVKGKEVGCLRELENLVCNFEGQSDFVEYLNSRDKTRSLSTYDIFVGPLDEDFYSEMKRGYGKSKTVWLSSLCNNGDGNFQVMFPNDIQKLIIFNCSCDVSSLIEHSIKLEVIHIKDCNSMESLISSSLFCPSPTSLPSYNGVFSTLKEFNCYGCSSMKKLFPLVLLPNLVNLVNITVIDCAKMEEIIGGRRSDEENSSNNTEFKLPKLRSLALSWLPKLKRICSAKLICDSLQQIEVSYCNSMESLVPSSWISLVNLEKITVRGCQKMEEIIGGRRSDEESSSNNTEFKLPKLRSLALEDLPELKSICSAKLTCDSLQQIEVWNCNSMESLVPSSWISLVNLEKITVRGGEKMEEIIGGRRSDEESSSTEFKLPKLRSLALFNLPELKSICSAKLTCDSLQQIEVWNCNSMEILVPSSWISLVNLEKITVSACKKMKEIIGGTRSDEESSSNNTEFKLPKLRSLALSWLPELKRICSAKLICDSLRMIEVYKCQKLKRMPLWKKDYTSKRMVGVSSGVGAS

Target ORF information:

RefSeq Version	XM_024591913.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024591913.1

ORF Insert Sequence:

ATGAGTCGAC CAAATGATCC TTTTTGGGAA TATGCTGAAA AGATGGATGA TGGTAGCATG 
ACGTGTACGT TTTGTGGGCA TTGTTTTTCC CAAGGTACTT CCGTTACGAG GATCAAATTG 
CATTTGGCAG GAGTGAAACG GCGTGGTGTT AAAATTTGTA AAAATGTTCC AGAAGAAGTT 
CGAGATGCAG CTTGTGATGC AGTTAATAAT AGCCCTCCAG AAAAGAAACT TAAATCTGTT 
GCGGGCTCAA GCAGTAACGA GGTCGCTAAT GCAATTTCAG CTTCAACGCA AGAACAGAAC 
AATGAAGTGA CCCATGTAGA GATGGCACAA CAAGGAGGAC CTTTTTTCAC TGGAGAACTT 
GCATGGGCAA ATGATTTGAT TGGAGAGGCT GAACTTGTGC AGCTGGAGAG AGGTAGTTCT 
CATGAGAGGC CATCAATTAA TCAAACAGAT GAGCCTCGAG GAGATTCATC CCGACCAACA 
GATGATCAAC TCTGTTCTCC ATCAGTAAAC AATGATGCCA ATATGAACGA TGTGCAGAAC 
ATGGTTGCAG TCAGGATAGA ACCAGTACCG GTTCAAGTGT TGGAACAAAG CAATGCAGAA 
GTTGACAGTT TGGCAGGGCA TGCTGGAAGG ATACAAGTGG GAGTTCATGG CATGGAACAA 
GGTGCGGAGG AAGAGAGAAT TTGTCCGAAC TCAGCGGCAA ATGGCATGGA AAACACTGGT 
GATGGATCTA GTCAGCATTC CAGGAGACTC ATGATTGATG CACATGATAA TACAGGGGAA 
GAAGCTACAC AAAGAATAGA TCTAGTGCAA CGATTAGAAG GGGAAACTTG GGGTCAGATG 
AATGCAATCT CTGCTTTTTT AATGGGGGAG GAGGACGTGG AGAACAATCG TGGAAGATTA 
GTGCAGCCTG GAGCAGCTTG CTCTTCTGGA GGGGCTGCAT GCAACACAAA TAAGATTAAA 
GGAGATGCAC TGCCAACGAG AAAAATGGTG GGTCAAGCAT TCGAAGAACA TAAGAAGACT 
ATCTCATCTT TGTTGATGCG CAATGAAGTT TCAAGTATTG GTATCTATGG GATGGGCGGA 
GTGGGAAAGA CCACATTGGG GACACATATA CATAATCAGC TTCTAGAAAG ACCAGAGACT 
CCTGTTTACT GGATTACTGT TTCACATAAT ACCAGCATTC CTAGATTGCA GACAAGTCTC 
GCAGGACGAA TTGGTTTAGA TCTTTCCAAG GTAGATGAGG AGCTGCATAG AGCGGTAGCG 
TTGAAAAAAG AATTAATGAA GAAACAGAAA TGGATTCTGA TTTTAGATGA TTTGTGGAAG 
GCTTTTGACC TGCAAAAGCT GGGAGTTCCT GACCAAGTTG AGGAGGGATG CAAGCTGATT 
CTTACATCTC GATCAGCAAA GGTTTGTCAA CAGATGAAAA CCCAACACAC GATAAAAGTG 
CAGCCTATTT CGGAGAAAGA AGCTTGGACT TTGTTTATTG AGAGACTTGG ACATGACATA 
GCATTTTCTT CAGAAGTGGA AGGAATTGCT TTGAATGTTG TAAGGGAATG TGCTGGTTTG 
CCATTGGGAA TTATTACAAT AGCAGCAAGC ATGAGAGGAG TGGATGAGCC ACATGAGTGG 
AGGAATACAT TGAAGAAATT GAAAGAATCA AAATATAAGG AAATGGAAGA TGAGGTATTC 
CGATTATTGA GGTTTAGTTA TGATCAGCTA AATGATTTAG CATTACAACA ATGTCTCTTG 
TACTGTGCAT TGTATCCTGA AGATCATAGG ATTGAAAGGG AGGAGCTGAT AGGTTATTTG 
ATCGACGAGG AAATAATTGA AGGAATGAGG AGCAGGCAAG CAGCATTTGA CGAGGGCCGC 
ACGATGCTTG ATAAACTTGA AAAAGTCTGT TTATTGGAAA GAGCTTGTTA TGGTGATCAT 
AGTACAACTG TCAAGATGCA TGACTTGATT AGGGACATGG CCCACCAAAT ACTCCAAACA 
AACTCTCCAG TCATGGTTGG GGGATATAAT GATAAATTAC CAGATGTGGA TATGTGGAAA 
GAGAATCTAG TGAGAGTCTC TCTGAAGCAT TGTTATTTCG AGGAAATTCC TTCTAGTCAT 
TCACCAAGAT GTCCCAATCT TTCAACTCTA TTGCTGTGTG ATAATCCTTA TTTGCAATTT 
ATTGCAGATT CTTTTTTCAC ACAATTGCAT GGGCTCAAGG TTCTTGATCT GTCTCGTACA 
GAAATCATAG AATTGCCTGA TTCTGTCTCT GAACTGGTGA GTCTCACTGC ATTATTGCTC 
AAACAATGTG AATATTTAAT ACATGTACCA TCATTAGAAA AGCTCACAGC ACTGAGGAGG 
CTAGATCTCT CTGGTACTTG GGAACTTGAA AAGATACCTC AAGACATGCA ATGTCTATCT 
AACCTGAGGT ATCTGAGGAT GGATGGATGT GGTGTAAAGG AGTTTCCTAC TGGGATATTA 
CCAAAACTCT TTCACCTACA ACTCTTCATG TTAGAGGGGA AGACTAATTA TGACTACGTT 
CAAGTAACAG TTAAGGGAAA GGAAGTAGGA TGCTTGAGGG AGTTGGAAAA TTTGGTATGC 
AATTTTGAAG GTCAATCTGA CTTTGTGGAG TATCTCAATT CTCGGGATAA GACCCGATCA 
CTAAGTACAT ACGATATTTT TGTAGGACCA CTGGATGAAG ATTTTTATAG TGAAATGAAG 
CGTGGTTATG GCAAAAGTAA AACAGTTTGG TTGAGTAGTT TGTGTAACAA TGGAGATGGA 
AATTTTCAAG TAATGTTCCC AAATGACATT CAAAAACTGA TTATTTTTAA TTGTTCATGT 
GATGTTTCCT CTTTAATAGA GCATTCAATT AAACTGGAGG TCATCCACAT TAAGGATTGC 
AATAGCATGG AGAGCTTGAT TTCATCTTCT TTGTTCTGCC CTTCTCCAAC ATCATTGCCA 
TCTTATAATG GTGTATTTTC TACTCTTAAA GAGTTTAATT GTTATGGATG TAGTAGTATG 
AAGAAGTTGT TCCCTCTTGT CTTGCTGCCA AACCTCGTAA ACCTAGTAAA TATTACAGTT 
ATAGATTGTG CAAAAATGGA GGAGATAATA GGTGGAAGAA GATCAGATGA AGAAAACAGC 
AGCAACAACA CCGAATTCAA GCTCCCAAAG TTAAGATCTC TGGCATTGAG TTGGTTACCA 
AAACTGAAAA GAATTTGTAG TGCAAAACTG ATTTGCGATT CTCTCCAACA AATTGAAGTA 
AGTTACTGCA ATAGCATGGA GAGCTTGGTT CCATCTTCTT GGATTTCCCT CGTAAACCTA 
GAAAAGATTA CAGTTAGAGG ATGTCAGAAG ATGGAGGAGA TAATAGGTGG AAGAAGATCA 
GATGAAGAAA GCAGCAGCAA CAACACCGAA TTCAAACTCC CAAAGTTAAG ATCTCTGGCA 
TTGGAAGATT TACCAGAACT GAAAAGCATT TGTAGTGCAA AGCTGACGTG CGATTCTCTC 
CAACAAATTG AAGTATGGAA TTGCAACAGC ATGGAGAGCT TGGTTCCATC TTCTTGGATT 
TCCCTCGTAA ACCTAGAAAA GATTACAGTT AGAGGAGGTG AGAAAATGGA GGAGATAATA 
GGTGGAAGAA GATCAGATGA AGAAAGCAGC AGCACCGAAT TCAAACTCCC AAAGTTAAGA 
TCTCTGGCAT TGTTTAATTT ACCAGAACTG AAAAGCATTT GTAGTGCAAA GCTGACGTGC 
GATTCTCTCC AACAAATTGA AGTATGGAAT TGCAACAGCA TGGAGATCTT GGTTCCATCT 
TCTTGGATTT CCCTTGTAAA CCTAGAAAAG ATTACAGTTA GTGCATGTAA GAAGATGAAG 
GAGATAATAG GTGGAACAAG ATCAGATGAA GAAAGTAGCA GTAACAACAC CGAATTCAAA 
CTCCCAAAGT TAAGATCTCT GGCATTGAGT TGGTTACCAG AACTGAAAAG AATTTGTAGT 
GCAAAACTGA TTTGCGATTC TCTCAGAATG ATTGAAGTTT ATAAATGTCA GAAGCTGAAG 
AGGATGCCAT TGTGGAAGAA GGATTATACA TCCAAGAGAA TGGTGGGAGT CAGTAGTGGT 
GTGGGAGCAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18107958 ( XM_024591916.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024447684.1 Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OPa418670
Clone ID Related Accession (Same CDS sequence)	XM_024591920.1 , XM_024591911.1 , XM_024591912.1 , XM_024591917.1 , XM_024591913.1 , XM_024591916.1 , XM_024591918.1 , XM_024591919.1 , XM_024591910.1 , XM_024591914.1
Accession Version	XM_024591916.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	probable disease resistance protein At4g27220 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020167869.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCGAC CAAATGATCC TTTTTGGGAA TATGCTGAAA AGATGGATGA TGGTAGCATG 
ACGTGTACGT TTTGTGGGCA TTGTTTTTCC CAAGGTACTT CCGTTACGAG GATCAAATTG 
CATTTGGCAG GAGTGAAACG GCGTGGTGTT AAAATTTGTA AAAATGTTCC AGAAGAAGTT 
CGAGATGCAG CTTGTGATGC AGTTAATAAT AGCCCTCCAG AAAAGAAACT TAAATCTGTT 
GCGGGCTCAA GCAGTAACGA GGTCGCTAAT GCAATTTCAG CTTCAACGCA AGAACAGAAC 
AATGAAGTGA CCCATGTAGA GATGGCACAA CAAGGAGGAC CTTTTTTCAC TGGAGAACTT 
GCATGGGCAA ATGATTTGAT TGGAGAGGCT GAACTTGTGC AGCTGGAGAG AGGTAGTTCT 
CATGAGAGGC CATCAATTAA TCAAACAGAT GAGCCTCGAG GAGATTCATC CCGACCAACA 
GATGATCAAC TCTGTTCTCC ATCAGTAAAC AATGATGCCA ATATGAACGA TGTGCAGAAC 
ATGGTTGCAG TCAGGATAGA ACCAGTACCG GTTCAAGTGT TGGAACAAAG CAATGCAGAA 
GTTGACAGTT TGGCAGGGCA TGCTGGAAGG ATACAAGTGG GAGTTCATGG CATGGAACAA 
GGTGCGGAGG AAGAGAGAAT TTGTCCGAAC TCAGCGGCAA ATGGCATGGA AAACACTGGT 
GATGGATCTA GTCAGCATTC CAGGAGACTC ATGATTGATG CACATGATAA TACAGGGGAA 
GAAGCTACAC AAAGAATAGA TCTAGTGCAA CGATTAGAAG GGGAAACTTG GGGTCAGATG 
AATGCAATCT CTGCTTTTTT AATGGGGGAG GAGGACGTGG AGAACAATCG TGGAAGATTA 
GTGCAGCCTG GAGCAGCTTG CTCTTCTGGA GGGGCTGCAT GCAACACAAA TAAGATTAAA 
GGAGATGCAC TGCCAACGAG AAAAATGGTG GGTCAAGCAT TCGAAGAACA TAAGAAGACT 
ATCTCATCTT TGTTGATGCG CAATGAAGTT TCAAGTATTG GTATCTATGG GATGGGCGGA 
GTGGGAAAGA CCACATTGGG GACACATATA CATAATCAGC TTCTAGAAAG ACCAGAGACT 
CCTGTTTACT GGATTACTGT TTCACATAAT ACCAGCATTC CTAGATTGCA GACAAGTCTC 
GCAGGACGAA TTGGTTTAGA TCTTTCCAAG GTAGATGAGG AGCTGCATAG AGCGGTAGCG 
TTGAAAAAAG AATTAATGAA GAAACAGAAA TGGATTCTGA TTTTAGATGA TTTGTGGAAG 
GCTTTTGACC TGCAAAAGCT GGGAGTTCCT GACCAAGTTG AGGAGGGATG CAAGCTGATT 
CTTACATCTC GATCAGCAAA GGTTTGTCAA CAGATGAAAA CCCAACACAC GATAAAAGTG 
CAGCCTATTT CGGAGAAAGA AGCTTGGACT TTGTTTATTG AGAGACTTGG ACATGACATA 
GCATTTTCTT CAGAAGTGGA AGGAATTGCT TTGAATGTTG TAAGGGAATG TGCTGGTTTG 
CCATTGGGAA TTATTACAAT AGCAGCAAGC ATGAGAGGAG TGGATGAGCC ACATGAGTGG 
AGGAATACAT TGAAGAAATT GAAAGAATCA AAATATAAGG AAATGGAAGA TGAGGTATTC 
CGATTATTGA GGTTTAGTTA TGATCAGCTA AATGATTTAG CATTACAACA ATGTCTCTTG 
TACTGTGCAT TGTATCCTGA AGATCATAGG ATTGAAAGGG AGGAGCTGAT AGGTTATTTG 
ATCGACGAGG AAATAATTGA AGGAATGAGG AGCAGGCAAG CAGCATTTGA CGAGGGCCGC 
ACGATGCTTG ATAAACTTGA AAAAGTCTGT TTATTGGAAA GAGCTTGTTA TGGTGATCAT 
AGTACAACTG TCAAGATGCA TGACTTGATT AGGGACATGG CCCACCAAAT ACTCCAAACA 
AACTCTCCAG TCATGGTTGG GGGATATAAT GATAAATTAC CAGATGTGGA TATGTGGAAA 
GAGAATCTAG TGAGAGTCTC TCTGAAGCAT TGTTATTTCG AGGAAATTCC TTCTAGTCAT 
TCACCAAGAT GTCCCAATCT TTCAACTCTA TTGCTGTGTG ATAATCCTTA TTTGCAATTT 
ATTGCAGATT CTTTTTTCAC ACAATTGCAT GGGCTCAAGG TTCTTGATCT GTCTCGTACA 
GAAATCATAG AATTGCCTGA TTCTGTCTCT GAACTGGTGA GTCTCACTGC ATTATTGCTC 
AAACAATGTG AATATTTAAT ACATGTACCA TCATTAGAAA AGCTCACAGC ACTGAGGAGG 
CTAGATCTCT CTGGTACTTG GGAACTTGAA AAGATACCTC AAGACATGCA ATGTCTATCT 
AACCTGAGGT ATCTGAGGAT GGATGGATGT GGTGTAAAGG AGTTTCCTAC TGGGATATTA 
CCAAAACTCT TTCACCTACA ACTCTTCATG TTAGAGGGGA AGACTAATTA TGACTACGTT 
CAAGTAACAG TTAAGGGAAA GGAAGTAGGA TGCTTGAGGG AGTTGGAAAA TTTGGTATGC 
AATTTTGAAG GTCAATCTGA CTTTGTGGAG TATCTCAATT CTCGGGATAA GACCCGATCA 
CTAAGTACAT ACGATATTTT TGTAGGACCA CTGGATGAAG ATTTTTATAG TGAAATGAAG 
CGTGGTTATG GCAAAAGTAA AACAGTTTGG TTGAGTAGTT TGTGTAACAA TGGAGATGGA 
AATTTTCAAG TAATGTTCCC AAATGACATT CAAAAACTGA TTATTTTTAA TTGTTCATGT 
GATGTTTCCT CTTTAATAGA GCATTCAATT AAACTGGAGG TCATCCACAT TAAGGATTGC 
AATAGCATGG AGAGCTTGAT TTCATCTTCT TTGTTCTGCC CTTCTCCAAC ATCATTGCCA 
TCTTATAATG GTGTATTTTC TACTCTTAAA GAGTTTAATT GTTATGGATG TAGTAGTATG 
AAGAAGTTGT TCCCTCTTGT CTTGCTGCCA AACCTCGTAA ACCTAGTAAA TATTACAGTT 
ATAGATTGTG CAAAAATGGA GGAGATAATA GGTGGAAGAA GATCAGATGA AGAAAACAGC 
AGCAACAACA CCGAATTCAA GCTCCCAAAG TTAAGATCTC TGGCATTGAG TTGGTTACCA 
AAACTGAAAA GAATTTGTAG TGCAAAACTG ATTTGCGATT CTCTCCAACA AATTGAAGTA 
AGTTACTGCA ATAGCATGGA GAGCTTGGTT CCATCTTCTT GGATTTCCCT CGTAAACCTA 
GAAAAGATTA CAGTTAGAGG ATGTCAGAAG ATGGAGGAGA TAATAGGTGG AAGAAGATCA 
GATGAAGAAA GCAGCAGCAA CAACACCGAA TTCAAACTCC CAAAGTTAAG ATCTCTGGCA 
TTGGAAGATT TACCAGAACT GAAAAGCATT TGTAGTGCAA AGCTGACGTG CGATTCTCTC 
CAACAAATTG AAGTATGGAA TTGCAACAGC ATGGAGAGCT TGGTTCCATC TTCTTGGATT 
TCCCTCGTAA ACCTAGAAAA GATTACAGTT AGAGGAGGTG AGAAAATGGA GGAGATAATA 
GGTGGAAGAA GATCAGATGA AGAAAGCAGC AGCACCGAAT TCAAACTCCC AAAGTTAAGA 
TCTCTGGCAT TGTTTAATTT ACCAGAACTG AAAAGCATTT GTAGTGCAAA GCTGACGTGC 
GATTCTCTCC AACAAATTGA AGTATGGAAT TGCAACAGCA TGGAGATCTT GGTTCCATCT 
TCTTGGATTT CCCTTGTAAA CCTAGAAAAG ATTACAGTTA GTGCATGTAA GAAGATGAAG 
GAGATAATAG GTGGAACAAG ATCAGATGAA GAAAGTAGCA GTAACAACAC CGAATTCAAA 
CTCCCAAAGT TAAGATCTCT GGCATTGAGT TGGTTACCAG AACTGAAAAG AATTTGTAGT 
GCAAAACTGA TTTGCGATTC TCTCAGAATG ATTGAAGTTT ATAAATGTCA GAAGCTGAAG 
AGGATGCCAT TGTGGAAGAA GGATTATACA TCCAAGAGAA TGGTGGGAGT CAGTAGTGGT 
GTGGGAGCAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024447684.1
CDS	547..4641
Translation	MSRPNDPFWEYAEKMDDGSMTCTFCGHCFSQGTSVTRIKLHLAGVKRRGVKICKNVPEEVRDAACDAVNNSPPEKKLKSVAGSSSNEVANAISASTQEQNNEVTHVEMAQQGGPFFTGELAWANDLIGEAELVQLERGSSHERPSINQTDEPRGDSSRPTDDQLCSPSVNNDANMNDVQNMVAVRIEPVPVQVLEQSNAEVDSLAGHAGRIQVGVHGMEQGAEEERICPNSAANGMENTGDGSSQHSRRLMIDAHDNTGEEATQRIDLVQRLEGETWGQMNAISAFLMGEEDVENNRGRLVQPGAACSSGGAACNTNKIKGDALPTRKMVGQAFEEHKKTISSLLMRNEVSSIGIYGMGGVGKTTLGTHIHNQLLERPETPVYWITVSHNTSIPRLQTSLAGRIGLDLSKVDEELHRAVALKKELMKKQKWILILDDLWKAFDLQKLGVPDQVEEGCKLILTSRSAKVCQQMKTQHTIKVQPISEKEAWTLFIERLGHDIAFSSEVEGIALNVVRECAGLPLGIITIAASMRGVDEPHEWRNTLKKLKESKYKEMEDEVFRLLRFSYDQLNDLALQQCLLYCALYPEDHRIEREELIGYLIDEEIIEGMRSRQAAFDEGRTMLDKLEKVCLLERACYGDHSTTVKMHDLIRDMAHQILQTNSPVMVGGYNDKLPDVDMWKENLVRVSLKHCYFEEIPSSHSPRCPNLSTLLLCDNPYLQFIADSFFTQLHGLKVLDLSRTEIIELPDSVSELVSLTALLLKQCEYLIHVPSLEKLTALRRLDLSGTWELEKIPQDMQCLSNLRYLRMDGCGVKEFPTGILPKLFHLQLFMLEGKTNYDYVQVTVKGKEVGCLRELENLVCNFEGQSDFVEYLNSRDKTRSLSTYDIFVGPLDEDFYSEMKRGYGKSKTVWLSSLCNNGDGNFQVMFPNDIQKLIIFNCSCDVSSLIEHSIKLEVIHIKDCNSMESLISSSLFCPSPTSLPSYNGVFSTLKEFNCYGCSSMKKLFPLVLLPNLVNLVNITVIDCAKMEEIIGGRRSDEENSSNNTEFKLPKLRSLALSWLPKLKRICSAKLICDSLQQIEVSYCNSMESLVPSSWISLVNLEKITVRGCQKMEEIIGGRRSDEESSSNNTEFKLPKLRSLALEDLPELKSICSAKLTCDSLQQIEVWNCNSMESLVPSSWISLVNLEKITVRGGEKMEEIIGGRRSDEESSSTEFKLPKLRSLALFNLPELKSICSAKLTCDSLQQIEVWNCNSMEILVPSSWISLVNLEKITVSACKKMKEIIGGTRSDEESSSNNTEFKLPKLRSLALSWLPELKRICSAKLICDSLRMIEVYKCQKLKRMPLWKKDYTSKRMVGVSSGVGAS

Target ORF information:

RefSeq Version	XM_024591916.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024591916.1

ORF Insert Sequence:

ATGAGTCGAC CAAATGATCC TTTTTGGGAA TATGCTGAAA AGATGGATGA TGGTAGCATG 
ACGTGTACGT TTTGTGGGCA TTGTTTTTCC CAAGGTACTT CCGTTACGAG GATCAAATTG 
CATTTGGCAG GAGTGAAACG GCGTGGTGTT AAAATTTGTA AAAATGTTCC AGAAGAAGTT 
CGAGATGCAG CTTGTGATGC AGTTAATAAT AGCCCTCCAG AAAAGAAACT TAAATCTGTT 
GCGGGCTCAA GCAGTAACGA GGTCGCTAAT GCAATTTCAG CTTCAACGCA AGAACAGAAC 
AATGAAGTGA CCCATGTAGA GATGGCACAA CAAGGAGGAC CTTTTTTCAC TGGAGAACTT 
GCATGGGCAA ATGATTTGAT TGGAGAGGCT GAACTTGTGC AGCTGGAGAG AGGTAGTTCT 
CATGAGAGGC CATCAATTAA TCAAACAGAT GAGCCTCGAG GAGATTCATC CCGACCAACA 
GATGATCAAC TCTGTTCTCC ATCAGTAAAC AATGATGCCA ATATGAACGA TGTGCAGAAC 
ATGGTTGCAG TCAGGATAGA ACCAGTACCG GTTCAAGTGT TGGAACAAAG CAATGCAGAA 
GTTGACAGTT TGGCAGGGCA TGCTGGAAGG ATACAAGTGG GAGTTCATGG CATGGAACAA 
GGTGCGGAGG AAGAGAGAAT TTGTCCGAAC TCAGCGGCAA ATGGCATGGA AAACACTGGT 
GATGGATCTA GTCAGCATTC CAGGAGACTC ATGATTGATG CACATGATAA TACAGGGGAA 
GAAGCTACAC AAAGAATAGA TCTAGTGCAA CGATTAGAAG GGGAAACTTG GGGTCAGATG 
AATGCAATCT CTGCTTTTTT AATGGGGGAG GAGGACGTGG AGAACAATCG TGGAAGATTA 
GTGCAGCCTG GAGCAGCTTG CTCTTCTGGA GGGGCTGCAT GCAACACAAA TAAGATTAAA 
GGAGATGCAC TGCCAACGAG AAAAATGGTG GGTCAAGCAT TCGAAGAACA TAAGAAGACT 
ATCTCATCTT TGTTGATGCG CAATGAAGTT TCAAGTATTG GTATCTATGG GATGGGCGGA 
GTGGGAAAGA CCACATTGGG GACACATATA CATAATCAGC TTCTAGAAAG ACCAGAGACT 
CCTGTTTACT GGATTACTGT TTCACATAAT ACCAGCATTC CTAGATTGCA GACAAGTCTC 
GCAGGACGAA TTGGTTTAGA TCTTTCCAAG GTAGATGAGG AGCTGCATAG AGCGGTAGCG 
TTGAAAAAAG AATTAATGAA GAAACAGAAA TGGATTCTGA TTTTAGATGA TTTGTGGAAG 
GCTTTTGACC TGCAAAAGCT GGGAGTTCCT GACCAAGTTG AGGAGGGATG CAAGCTGATT 
CTTACATCTC GATCAGCAAA GGTTTGTCAA CAGATGAAAA CCCAACACAC GATAAAAGTG 
CAGCCTATTT CGGAGAAAGA AGCTTGGACT TTGTTTATTG AGAGACTTGG ACATGACATA 
GCATTTTCTT CAGAAGTGGA AGGAATTGCT TTGAATGTTG TAAGGGAATG TGCTGGTTTG 
CCATTGGGAA TTATTACAAT AGCAGCAAGC ATGAGAGGAG TGGATGAGCC ACATGAGTGG 
AGGAATACAT TGAAGAAATT GAAAGAATCA AAATATAAGG AAATGGAAGA TGAGGTATTC 
CGATTATTGA GGTTTAGTTA TGATCAGCTA AATGATTTAG CATTACAACA ATGTCTCTTG 
TACTGTGCAT TGTATCCTGA AGATCATAGG ATTGAAAGGG AGGAGCTGAT AGGTTATTTG 
ATCGACGAGG AAATAATTGA AGGAATGAGG AGCAGGCAAG CAGCATTTGA CGAGGGCCGC 
ACGATGCTTG ATAAACTTGA AAAAGTCTGT TTATTGGAAA GAGCTTGTTA TGGTGATCAT 
AGTACAACTG TCAAGATGCA TGACTTGATT AGGGACATGG CCCACCAAAT ACTCCAAACA 
AACTCTCCAG TCATGGTTGG GGGATATAAT GATAAATTAC CAGATGTGGA TATGTGGAAA 
GAGAATCTAG TGAGAGTCTC TCTGAAGCAT TGTTATTTCG AGGAAATTCC TTCTAGTCAT 
TCACCAAGAT GTCCCAATCT TTCAACTCTA TTGCTGTGTG ATAATCCTTA TTTGCAATTT 
ATTGCAGATT CTTTTTTCAC ACAATTGCAT GGGCTCAAGG TTCTTGATCT GTCTCGTACA 
GAAATCATAG AATTGCCTGA TTCTGTCTCT GAACTGGTGA GTCTCACTGC ATTATTGCTC 
AAACAATGTG AATATTTAAT ACATGTACCA TCATTAGAAA AGCTCACAGC ACTGAGGAGG 
CTAGATCTCT CTGGTACTTG GGAACTTGAA AAGATACCTC AAGACATGCA ATGTCTATCT 
AACCTGAGGT ATCTGAGGAT GGATGGATGT GGTGTAAAGG AGTTTCCTAC TGGGATATTA 
CCAAAACTCT TTCACCTACA ACTCTTCATG TTAGAGGGGA AGACTAATTA TGACTACGTT 
CAAGTAACAG TTAAGGGAAA GGAAGTAGGA TGCTTGAGGG AGTTGGAAAA TTTGGTATGC 
AATTTTGAAG GTCAATCTGA CTTTGTGGAG TATCTCAATT CTCGGGATAA GACCCGATCA 
CTAAGTACAT ACGATATTTT TGTAGGACCA CTGGATGAAG ATTTTTATAG TGAAATGAAG 
CGTGGTTATG GCAAAAGTAA AACAGTTTGG TTGAGTAGTT TGTGTAACAA TGGAGATGGA 
AATTTTCAAG TAATGTTCCC AAATGACATT CAAAAACTGA TTATTTTTAA TTGTTCATGT 
GATGTTTCCT CTTTAATAGA GCATTCAATT AAACTGGAGG TCATCCACAT TAAGGATTGC 
AATAGCATGG AGAGCTTGAT TTCATCTTCT TTGTTCTGCC CTTCTCCAAC ATCATTGCCA 
TCTTATAATG GTGTATTTTC TACTCTTAAA GAGTTTAATT GTTATGGATG TAGTAGTATG 
AAGAAGTTGT TCCCTCTTGT CTTGCTGCCA AACCTCGTAA ACCTAGTAAA TATTACAGTT 
ATAGATTGTG CAAAAATGGA GGAGATAATA GGTGGAAGAA GATCAGATGA AGAAAACAGC 
AGCAACAACA CCGAATTCAA GCTCCCAAAG TTAAGATCTC TGGCATTGAG TTGGTTACCA 
AAACTGAAAA GAATTTGTAG TGCAAAACTG ATTTGCGATT CTCTCCAACA AATTGAAGTA 
AGTTACTGCA ATAGCATGGA GAGCTTGGTT CCATCTTCTT GGATTTCCCT CGTAAACCTA 
GAAAAGATTA CAGTTAGAGG ATGTCAGAAG ATGGAGGAGA TAATAGGTGG AAGAAGATCA 
GATGAAGAAA GCAGCAGCAA CAACACCGAA TTCAAACTCC CAAAGTTAAG ATCTCTGGCA 
TTGGAAGATT TACCAGAACT GAAAAGCATT TGTAGTGCAA AGCTGACGTG CGATTCTCTC 
CAACAAATTG AAGTATGGAA TTGCAACAGC ATGGAGAGCT TGGTTCCATC TTCTTGGATT 
TCCCTCGTAA ACCTAGAAAA GATTACAGTT AGAGGAGGTG AGAAAATGGA GGAGATAATA 
GGTGGAAGAA GATCAGATGA AGAAAGCAGC AGCACCGAAT TCAAACTCCC AAAGTTAAGA 
TCTCTGGCAT TGTTTAATTT ACCAGAACTG AAAAGCATTT GTAGTGCAAA GCTGACGTGC 
GATTCTCTCC AACAAATTGA AGTATGGAAT TGCAACAGCA TGGAGATCTT GGTTCCATCT 
TCTTGGATTT CCCTTGTAAA CCTAGAAAAG ATTACAGTTA GTGCATGTAA GAAGATGAAG 
GAGATAATAG GTGGAACAAG ATCAGATGAA GAAAGTAGCA GTAACAACAC CGAATTCAAA 
CTCCCAAAGT TAAGATCTCT GGCATTGAGT TGGTTACCAG AACTGAAAAG AATTTGTAGT 
GCAAAACTGA TTTGCGATTC TCTCAGAATG ATTGAAGTTT ATAAATGTCA GAAGCTGAAG 
AGGATGCCAT TGTGGAAGAA GGATTATACA TCCAAGAGAA TGGTGGGAGT CAGTAGTGGT 
GTGGGAGCAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18107958 ( XM_024591918.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024447686.1 Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OPa418670
Clone ID Related Accession (Same CDS sequence)	XM_024591920.1 , XM_024591911.1 , XM_024591912.1 , XM_024591917.1 , XM_024591913.1 , XM_024591916.1 , XM_024591918.1 , XM_024591919.1 , XM_024591910.1 , XM_024591914.1
Accession Version	XM_024591918.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	probable disease resistance protein At4g27220 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020167869.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCGAC CAAATGATCC TTTTTGGGAA TATGCTGAAA AGATGGATGA TGGTAGCATG 
ACGTGTACGT TTTGTGGGCA TTGTTTTTCC CAAGGTACTT CCGTTACGAG GATCAAATTG 
CATTTGGCAG GAGTGAAACG GCGTGGTGTT AAAATTTGTA AAAATGTTCC AGAAGAAGTT 
CGAGATGCAG CTTGTGATGC AGTTAATAAT AGCCCTCCAG AAAAGAAACT TAAATCTGTT 
GCGGGCTCAA GCAGTAACGA GGTCGCTAAT GCAATTTCAG CTTCAACGCA AGAACAGAAC 
AATGAAGTGA CCCATGTAGA GATGGCACAA CAAGGAGGAC CTTTTTTCAC TGGAGAACTT 
GCATGGGCAA ATGATTTGAT TGGAGAGGCT GAACTTGTGC AGCTGGAGAG AGGTAGTTCT 
CATGAGAGGC CATCAATTAA TCAAACAGAT GAGCCTCGAG GAGATTCATC CCGACCAACA 
GATGATCAAC TCTGTTCTCC ATCAGTAAAC AATGATGCCA ATATGAACGA TGTGCAGAAC 
ATGGTTGCAG TCAGGATAGA ACCAGTACCG GTTCAAGTGT TGGAACAAAG CAATGCAGAA 
GTTGACAGTT TGGCAGGGCA TGCTGGAAGG ATACAAGTGG GAGTTCATGG CATGGAACAA 
GGTGCGGAGG AAGAGAGAAT TTGTCCGAAC TCAGCGGCAA ATGGCATGGA AAACACTGGT 
GATGGATCTA GTCAGCATTC CAGGAGACTC ATGATTGATG CACATGATAA TACAGGGGAA 
GAAGCTACAC AAAGAATAGA TCTAGTGCAA CGATTAGAAG GGGAAACTTG GGGTCAGATG 
AATGCAATCT CTGCTTTTTT AATGGGGGAG GAGGACGTGG AGAACAATCG TGGAAGATTA 
GTGCAGCCTG GAGCAGCTTG CTCTTCTGGA GGGGCTGCAT GCAACACAAA TAAGATTAAA 
GGAGATGCAC TGCCAACGAG AAAAATGGTG GGTCAAGCAT TCGAAGAACA TAAGAAGACT 
ATCTCATCTT TGTTGATGCG CAATGAAGTT TCAAGTATTG GTATCTATGG GATGGGCGGA 
GTGGGAAAGA CCACATTGGG GACACATATA CATAATCAGC TTCTAGAAAG ACCAGAGACT 
CCTGTTTACT GGATTACTGT TTCACATAAT ACCAGCATTC CTAGATTGCA GACAAGTCTC 
GCAGGACGAA TTGGTTTAGA TCTTTCCAAG GTAGATGAGG AGCTGCATAG AGCGGTAGCG 
TTGAAAAAAG AATTAATGAA GAAACAGAAA TGGATTCTGA TTTTAGATGA TTTGTGGAAG 
GCTTTTGACC TGCAAAAGCT GGGAGTTCCT GACCAAGTTG AGGAGGGATG CAAGCTGATT 
CTTACATCTC GATCAGCAAA GGTTTGTCAA CAGATGAAAA CCCAACACAC GATAAAAGTG 
CAGCCTATTT CGGAGAAAGA AGCTTGGACT TTGTTTATTG AGAGACTTGG ACATGACATA 
GCATTTTCTT CAGAAGTGGA AGGAATTGCT TTGAATGTTG TAAGGGAATG TGCTGGTTTG 
CCATTGGGAA TTATTACAAT AGCAGCAAGC ATGAGAGGAG TGGATGAGCC ACATGAGTGG 
AGGAATACAT TGAAGAAATT GAAAGAATCA AAATATAAGG AAATGGAAGA TGAGGTATTC 
CGATTATTGA GGTTTAGTTA TGATCAGCTA AATGATTTAG CATTACAACA ATGTCTCTTG 
TACTGTGCAT TGTATCCTGA AGATCATAGG ATTGAAAGGG AGGAGCTGAT AGGTTATTTG 
ATCGACGAGG AAATAATTGA AGGAATGAGG AGCAGGCAAG CAGCATTTGA CGAGGGCCGC 
ACGATGCTTG ATAAACTTGA AAAAGTCTGT TTATTGGAAA GAGCTTGTTA TGGTGATCAT 
AGTACAACTG TCAAGATGCA TGACTTGATT AGGGACATGG CCCACCAAAT ACTCCAAACA 
AACTCTCCAG TCATGGTTGG GGGATATAAT GATAAATTAC CAGATGTGGA TATGTGGAAA 
GAGAATCTAG TGAGAGTCTC TCTGAAGCAT TGTTATTTCG AGGAAATTCC TTCTAGTCAT 
TCACCAAGAT GTCCCAATCT TTCAACTCTA TTGCTGTGTG ATAATCCTTA TTTGCAATTT 
ATTGCAGATT CTTTTTTCAC ACAATTGCAT GGGCTCAAGG TTCTTGATCT GTCTCGTACA 
GAAATCATAG AATTGCCTGA TTCTGTCTCT GAACTGGTGA GTCTCACTGC ATTATTGCTC 
AAACAATGTG AATATTTAAT ACATGTACCA TCATTAGAAA AGCTCACAGC ACTGAGGAGG 
CTAGATCTCT CTGGTACTTG GGAACTTGAA AAGATACCTC AAGACATGCA ATGTCTATCT 
AACCTGAGGT ATCTGAGGAT GGATGGATGT GGTGTAAAGG AGTTTCCTAC TGGGATATTA 
CCAAAACTCT TTCACCTACA ACTCTTCATG TTAGAGGGGA AGACTAATTA TGACTACGTT 
CAAGTAACAG TTAAGGGAAA GGAAGTAGGA TGCTTGAGGG AGTTGGAAAA TTTGGTATGC 
AATTTTGAAG GTCAATCTGA CTTTGTGGAG TATCTCAATT CTCGGGATAA GACCCGATCA 
CTAAGTACAT ACGATATTTT TGTAGGACCA CTGGATGAAG ATTTTTATAG TGAAATGAAG 
CGTGGTTATG GCAAAAGTAA AACAGTTTGG TTGAGTAGTT TGTGTAACAA TGGAGATGGA 
AATTTTCAAG TAATGTTCCC AAATGACATT CAAAAACTGA TTATTTTTAA TTGTTCATGT 
GATGTTTCCT CTTTAATAGA GCATTCAATT AAACTGGAGG TCATCCACAT TAAGGATTGC 
AATAGCATGG AGAGCTTGAT TTCATCTTCT TTGTTCTGCC CTTCTCCAAC ATCATTGCCA 
TCTTATAATG GTGTATTTTC TACTCTTAAA GAGTTTAATT GTTATGGATG TAGTAGTATG 
AAGAAGTTGT TCCCTCTTGT CTTGCTGCCA AACCTCGTAA ACCTAGTAAA TATTACAGTT 
ATAGATTGTG CAAAAATGGA GGAGATAATA GGTGGAAGAA GATCAGATGA AGAAAACAGC 
AGCAACAACA CCGAATTCAA GCTCCCAAAG TTAAGATCTC TGGCATTGAG TTGGTTACCA 
AAACTGAAAA GAATTTGTAG TGCAAAACTG ATTTGCGATT CTCTCCAACA AATTGAAGTA 
AGTTACTGCA ATAGCATGGA GAGCTTGGTT CCATCTTCTT GGATTTCCCT CGTAAACCTA 
GAAAAGATTA CAGTTAGAGG ATGTCAGAAG ATGGAGGAGA TAATAGGTGG AAGAAGATCA 
GATGAAGAAA GCAGCAGCAA CAACACCGAA TTCAAACTCC CAAAGTTAAG ATCTCTGGCA 
TTGGAAGATT TACCAGAACT GAAAAGCATT TGTAGTGCAA AGCTGACGTG CGATTCTCTC 
CAACAAATTG AAGTATGGAA TTGCAACAGC ATGGAGAGCT TGGTTCCATC TTCTTGGATT 
TCCCTCGTAA ACCTAGAAAA GATTACAGTT AGAGGAGGTG AGAAAATGGA GGAGATAATA 
GGTGGAAGAA GATCAGATGA AGAAAGCAGC AGCACCGAAT TCAAACTCCC AAAGTTAAGA 
TCTCTGGCAT TGTTTAATTT ACCAGAACTG AAAAGCATTT GTAGTGCAAA GCTGACGTGC 
GATTCTCTCC AACAAATTGA AGTATGGAAT TGCAACAGCA TGGAGATCTT GGTTCCATCT 
TCTTGGATTT CCCTTGTAAA CCTAGAAAAG ATTACAGTTA GTGCATGTAA GAAGATGAAG 
GAGATAATAG GTGGAACAAG ATCAGATGAA GAAAGTAGCA GTAACAACAC CGAATTCAAA 
CTCCCAAAGT TAAGATCTCT GGCATTGAGT TGGTTACCAG AACTGAAAAG AATTTGTAGT 
GCAAAACTGA TTTGCGATTC TCTCAGAATG ATTGAAGTTT ATAAATGTCA GAAGCTGAAG 
AGGATGCCAT TGTGGAAGAA GGATTATACA TCCAAGAGAA TGGTGGGAGT CAGTAGTGGT 
GTGGGAGCAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024447686.1
CDS	901..4995
Translation	MSRPNDPFWEYAEKMDDGSMTCTFCGHCFSQGTSVTRIKLHLAGVKRRGVKICKNVPEEVRDAACDAVNNSPPEKKLKSVAGSSSNEVANAISASTQEQNNEVTHVEMAQQGGPFFTGELAWANDLIGEAELVQLERGSSHERPSINQTDEPRGDSSRPTDDQLCSPSVNNDANMNDVQNMVAVRIEPVPVQVLEQSNAEVDSLAGHAGRIQVGVHGMEQGAEEERICPNSAANGMENTGDGSSQHSRRLMIDAHDNTGEEATQRIDLVQRLEGETWGQMNAISAFLMGEEDVENNRGRLVQPGAACSSGGAACNTNKIKGDALPTRKMVGQAFEEHKKTISSLLMRNEVSSIGIYGMGGVGKTTLGTHIHNQLLERPETPVYWITVSHNTSIPRLQTSLAGRIGLDLSKVDEELHRAVALKKELMKKQKWILILDDLWKAFDLQKLGVPDQVEEGCKLILTSRSAKVCQQMKTQHTIKVQPISEKEAWTLFIERLGHDIAFSSEVEGIALNVVRECAGLPLGIITIAASMRGVDEPHEWRNTLKKLKESKYKEMEDEVFRLLRFSYDQLNDLALQQCLLYCALYPEDHRIEREELIGYLIDEEIIEGMRSRQAAFDEGRTMLDKLEKVCLLERACYGDHSTTVKMHDLIRDMAHQILQTNSPVMVGGYNDKLPDVDMWKENLVRVSLKHCYFEEIPSSHSPRCPNLSTLLLCDNPYLQFIADSFFTQLHGLKVLDLSRTEIIELPDSVSELVSLTALLLKQCEYLIHVPSLEKLTALRRLDLSGTWELEKIPQDMQCLSNLRYLRMDGCGVKEFPTGILPKLFHLQLFMLEGKTNYDYVQVTVKGKEVGCLRELENLVCNFEGQSDFVEYLNSRDKTRSLSTYDIFVGPLDEDFYSEMKRGYGKSKTVWLSSLCNNGDGNFQVMFPNDIQKLIIFNCSCDVSSLIEHSIKLEVIHIKDCNSMESLISSSLFCPSPTSLPSYNGVFSTLKEFNCYGCSSMKKLFPLVLLPNLVNLVNITVIDCAKMEEIIGGRRSDEENSSNNTEFKLPKLRSLALSWLPKLKRICSAKLICDSLQQIEVSYCNSMESLVPSSWISLVNLEKITVRGCQKMEEIIGGRRSDEESSSNNTEFKLPKLRSLALEDLPELKSICSAKLTCDSLQQIEVWNCNSMESLVPSSWISLVNLEKITVRGGEKMEEIIGGRRSDEESSSTEFKLPKLRSLALFNLPELKSICSAKLTCDSLQQIEVWNCNSMEILVPSSWISLVNLEKITVSACKKMKEIIGGTRSDEESSSNNTEFKLPKLRSLALSWLPELKRICSAKLICDSLRMIEVYKCQKLKRMPLWKKDYTSKRMVGVSSGVGAS

Target ORF information:

RefSeq Version	XM_024591918.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024591918.1

ORF Insert Sequence:

ATGAGTCGAC CAAATGATCC TTTTTGGGAA TATGCTGAAA AGATGGATGA TGGTAGCATG 
ACGTGTACGT TTTGTGGGCA TTGTTTTTCC CAAGGTACTT CCGTTACGAG GATCAAATTG 
CATTTGGCAG GAGTGAAACG GCGTGGTGTT AAAATTTGTA AAAATGTTCC AGAAGAAGTT 
CGAGATGCAG CTTGTGATGC AGTTAATAAT AGCCCTCCAG AAAAGAAACT TAAATCTGTT 
GCGGGCTCAA GCAGTAACGA GGTCGCTAAT GCAATTTCAG CTTCAACGCA AGAACAGAAC 
AATGAAGTGA CCCATGTAGA GATGGCACAA CAAGGAGGAC CTTTTTTCAC TGGAGAACTT 
GCATGGGCAA ATGATTTGAT TGGAGAGGCT GAACTTGTGC AGCTGGAGAG AGGTAGTTCT 
CATGAGAGGC CATCAATTAA TCAAACAGAT GAGCCTCGAG GAGATTCATC CCGACCAACA 
GATGATCAAC TCTGTTCTCC ATCAGTAAAC AATGATGCCA ATATGAACGA TGTGCAGAAC 
ATGGTTGCAG TCAGGATAGA ACCAGTACCG GTTCAAGTGT TGGAACAAAG CAATGCAGAA 
GTTGACAGTT TGGCAGGGCA TGCTGGAAGG ATACAAGTGG GAGTTCATGG CATGGAACAA 
GGTGCGGAGG AAGAGAGAAT TTGTCCGAAC TCAGCGGCAA ATGGCATGGA AAACACTGGT 
GATGGATCTA GTCAGCATTC CAGGAGACTC ATGATTGATG CACATGATAA TACAGGGGAA 
GAAGCTACAC AAAGAATAGA TCTAGTGCAA CGATTAGAAG GGGAAACTTG GGGTCAGATG 
AATGCAATCT CTGCTTTTTT AATGGGGGAG GAGGACGTGG AGAACAATCG TGGAAGATTA 
GTGCAGCCTG GAGCAGCTTG CTCTTCTGGA GGGGCTGCAT GCAACACAAA TAAGATTAAA 
GGAGATGCAC TGCCAACGAG AAAAATGGTG GGTCAAGCAT TCGAAGAACA TAAGAAGACT 
ATCTCATCTT TGTTGATGCG CAATGAAGTT TCAAGTATTG GTATCTATGG GATGGGCGGA 
GTGGGAAAGA CCACATTGGG GACACATATA CATAATCAGC TTCTAGAAAG ACCAGAGACT 
CCTGTTTACT GGATTACTGT TTCACATAAT ACCAGCATTC CTAGATTGCA GACAAGTCTC 
GCAGGACGAA TTGGTTTAGA TCTTTCCAAG GTAGATGAGG AGCTGCATAG AGCGGTAGCG 
TTGAAAAAAG AATTAATGAA GAAACAGAAA TGGATTCTGA TTTTAGATGA TTTGTGGAAG 
GCTTTTGACC TGCAAAAGCT GGGAGTTCCT GACCAAGTTG AGGAGGGATG CAAGCTGATT 
CTTACATCTC GATCAGCAAA GGTTTGTCAA CAGATGAAAA CCCAACACAC GATAAAAGTG 
CAGCCTATTT CGGAGAAAGA AGCTTGGACT TTGTTTATTG AGAGACTTGG ACATGACATA 
GCATTTTCTT CAGAAGTGGA AGGAATTGCT TTGAATGTTG TAAGGGAATG TGCTGGTTTG 
CCATTGGGAA TTATTACAAT AGCAGCAAGC ATGAGAGGAG TGGATGAGCC ACATGAGTGG 
AGGAATACAT TGAAGAAATT GAAAGAATCA AAATATAAGG AAATGGAAGA TGAGGTATTC 
CGATTATTGA GGTTTAGTTA TGATCAGCTA AATGATTTAG CATTACAACA ATGTCTCTTG 
TACTGTGCAT TGTATCCTGA AGATCATAGG ATTGAAAGGG AGGAGCTGAT AGGTTATTTG 
ATCGACGAGG AAATAATTGA AGGAATGAGG AGCAGGCAAG CAGCATTTGA CGAGGGCCGC 
ACGATGCTTG ATAAACTTGA AAAAGTCTGT TTATTGGAAA GAGCTTGTTA TGGTGATCAT 
AGTACAACTG TCAAGATGCA TGACTTGATT AGGGACATGG CCCACCAAAT ACTCCAAACA 
AACTCTCCAG TCATGGTTGG GGGATATAAT GATAAATTAC CAGATGTGGA TATGTGGAAA 
GAGAATCTAG TGAGAGTCTC TCTGAAGCAT TGTTATTTCG AGGAAATTCC TTCTAGTCAT 
TCACCAAGAT GTCCCAATCT TTCAACTCTA TTGCTGTGTG ATAATCCTTA TTTGCAATTT 
ATTGCAGATT CTTTTTTCAC ACAATTGCAT GGGCTCAAGG TTCTTGATCT GTCTCGTACA 
GAAATCATAG AATTGCCTGA TTCTGTCTCT GAACTGGTGA GTCTCACTGC ATTATTGCTC 
AAACAATGTG AATATTTAAT ACATGTACCA TCATTAGAAA AGCTCACAGC ACTGAGGAGG 
CTAGATCTCT CTGGTACTTG GGAACTTGAA AAGATACCTC AAGACATGCA ATGTCTATCT 
AACCTGAGGT ATCTGAGGAT GGATGGATGT GGTGTAAAGG AGTTTCCTAC TGGGATATTA 
CCAAAACTCT TTCACCTACA ACTCTTCATG TTAGAGGGGA AGACTAATTA TGACTACGTT 
CAAGTAACAG TTAAGGGAAA GGAAGTAGGA TGCTTGAGGG AGTTGGAAAA TTTGGTATGC 
AATTTTGAAG GTCAATCTGA CTTTGTGGAG TATCTCAATT CTCGGGATAA GACCCGATCA 
CTAAGTACAT ACGATATTTT TGTAGGACCA CTGGATGAAG ATTTTTATAG TGAAATGAAG 
CGTGGTTATG GCAAAAGTAA AACAGTTTGG TTGAGTAGTT TGTGTAACAA TGGAGATGGA 
AATTTTCAAG TAATGTTCCC AAATGACATT CAAAAACTGA TTATTTTTAA TTGTTCATGT 
GATGTTTCCT CTTTAATAGA GCATTCAATT AAACTGGAGG TCATCCACAT TAAGGATTGC 
AATAGCATGG AGAGCTTGAT TTCATCTTCT TTGTTCTGCC CTTCTCCAAC ATCATTGCCA 
TCTTATAATG GTGTATTTTC TACTCTTAAA GAGTTTAATT GTTATGGATG TAGTAGTATG 
AAGAAGTTGT TCCCTCTTGT CTTGCTGCCA AACCTCGTAA ACCTAGTAAA TATTACAGTT 
ATAGATTGTG CAAAAATGGA GGAGATAATA GGTGGAAGAA GATCAGATGA AGAAAACAGC 
AGCAACAACA CCGAATTCAA GCTCCCAAAG TTAAGATCTC TGGCATTGAG TTGGTTACCA 
AAACTGAAAA GAATTTGTAG TGCAAAACTG ATTTGCGATT CTCTCCAACA AATTGAAGTA 
AGTTACTGCA ATAGCATGGA GAGCTTGGTT CCATCTTCTT GGATTTCCCT CGTAAACCTA 
GAAAAGATTA CAGTTAGAGG ATGTCAGAAG ATGGAGGAGA TAATAGGTGG AAGAAGATCA 
GATGAAGAAA GCAGCAGCAA CAACACCGAA TTCAAACTCC CAAAGTTAAG ATCTCTGGCA 
TTGGAAGATT TACCAGAACT GAAAAGCATT TGTAGTGCAA AGCTGACGTG CGATTCTCTC 
CAACAAATTG AAGTATGGAA TTGCAACAGC ATGGAGAGCT TGGTTCCATC TTCTTGGATT 
TCCCTCGTAA ACCTAGAAAA GATTACAGTT AGAGGAGGTG AGAAAATGGA GGAGATAATA 
GGTGGAAGAA GATCAGATGA AGAAAGCAGC AGCACCGAAT TCAAACTCCC AAAGTTAAGA 
TCTCTGGCAT TGTTTAATTT ACCAGAACTG AAAAGCATTT GTAGTGCAAA GCTGACGTGC 
GATTCTCTCC AACAAATTGA AGTATGGAAT TGCAACAGCA TGGAGATCTT GGTTCCATCT 
TCTTGGATTT CCCTTGTAAA CCTAGAAAAG ATTACAGTTA GTGCATGTAA GAAGATGAAG 
GAGATAATAG GTGGAACAAG ATCAGATGAA GAAAGTAGCA GTAACAACAC CGAATTCAAA 
CTCCCAAAGT TAAGATCTCT GGCATTGAGT TGGTTACCAG AACTGAAAAG AATTTGTAGT 
GCAAAACTGA TTTGCGATTC TCTCAGAATG ATTGAAGTTT ATAAATGTCA GAAGCTGAAG 
AGGATGCCAT TGTGGAAGAA GGATTATACA TCCAAGAGAA TGGTGGGAGT CAGTAGTGGT 
GTGGGAGCAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18107958 ( XM_024591919.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024447687.1 Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X9, mRNA.

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CloneID	OPa418670
Clone ID Related Accession (Same CDS sequence)	XM_024591920.1 , XM_024591911.1 , XM_024591912.1 , XM_024591917.1 , XM_024591913.1 , XM_024591916.1 , XM_024591918.1 , XM_024591919.1 , XM_024591910.1 , XM_024591914.1
Accession Version	XM_024591919.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	probable disease resistance protein At4g27220 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020167869.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCGAC CAAATGATCC TTTTTGGGAA TATGCTGAAA AGATGGATGA TGGTAGCATG 
ACGTGTACGT TTTGTGGGCA TTGTTTTTCC CAAGGTACTT CCGTTACGAG GATCAAATTG 
CATTTGGCAG GAGTGAAACG GCGTGGTGTT AAAATTTGTA AAAATGTTCC AGAAGAAGTT 
CGAGATGCAG CTTGTGATGC AGTTAATAAT AGCCCTCCAG AAAAGAAACT TAAATCTGTT 
GCGGGCTCAA GCAGTAACGA GGTCGCTAAT GCAATTTCAG CTTCAACGCA AGAACAGAAC 
AATGAAGTGA CCCATGTAGA GATGGCACAA CAAGGAGGAC CTTTTTTCAC TGGAGAACTT 
GCATGGGCAA ATGATTTGAT TGGAGAGGCT GAACTTGTGC AGCTGGAGAG AGGTAGTTCT 
CATGAGAGGC CATCAATTAA TCAAACAGAT GAGCCTCGAG GAGATTCATC CCGACCAACA 
GATGATCAAC TCTGTTCTCC ATCAGTAAAC AATGATGCCA ATATGAACGA TGTGCAGAAC 
ATGGTTGCAG TCAGGATAGA ACCAGTACCG GTTCAAGTGT TGGAACAAAG CAATGCAGAA 
GTTGACAGTT TGGCAGGGCA TGCTGGAAGG ATACAAGTGG GAGTTCATGG CATGGAACAA 
GGTGCGGAGG AAGAGAGAAT TTGTCCGAAC TCAGCGGCAA ATGGCATGGA AAACACTGGT 
GATGGATCTA GTCAGCATTC CAGGAGACTC ATGATTGATG CACATGATAA TACAGGGGAA 
GAAGCTACAC AAAGAATAGA TCTAGTGCAA CGATTAGAAG GGGAAACTTG GGGTCAGATG 
AATGCAATCT CTGCTTTTTT AATGGGGGAG GAGGACGTGG AGAACAATCG TGGAAGATTA 
GTGCAGCCTG GAGCAGCTTG CTCTTCTGGA GGGGCTGCAT GCAACACAAA TAAGATTAAA 
GGAGATGCAC TGCCAACGAG AAAAATGGTG GGTCAAGCAT TCGAAGAACA TAAGAAGACT 
ATCTCATCTT TGTTGATGCG CAATGAAGTT TCAAGTATTG GTATCTATGG GATGGGCGGA 
GTGGGAAAGA CCACATTGGG GACACATATA CATAATCAGC TTCTAGAAAG ACCAGAGACT 
CCTGTTTACT GGATTACTGT TTCACATAAT ACCAGCATTC CTAGATTGCA GACAAGTCTC 
GCAGGACGAA TTGGTTTAGA TCTTTCCAAG GTAGATGAGG AGCTGCATAG AGCGGTAGCG 
TTGAAAAAAG AATTAATGAA GAAACAGAAA TGGATTCTGA TTTTAGATGA TTTGTGGAAG 
GCTTTTGACC TGCAAAAGCT GGGAGTTCCT GACCAAGTTG AGGAGGGATG CAAGCTGATT 
CTTACATCTC GATCAGCAAA GGTTTGTCAA CAGATGAAAA CCCAACACAC GATAAAAGTG 
CAGCCTATTT CGGAGAAAGA AGCTTGGACT TTGTTTATTG AGAGACTTGG ACATGACATA 
GCATTTTCTT CAGAAGTGGA AGGAATTGCT TTGAATGTTG TAAGGGAATG TGCTGGTTTG 
CCATTGGGAA TTATTACAAT AGCAGCAAGC ATGAGAGGAG TGGATGAGCC ACATGAGTGG 
AGGAATACAT TGAAGAAATT GAAAGAATCA AAATATAAGG AAATGGAAGA TGAGGTATTC 
CGATTATTGA GGTTTAGTTA TGATCAGCTA AATGATTTAG CATTACAACA ATGTCTCTTG 
TACTGTGCAT TGTATCCTGA AGATCATAGG ATTGAAAGGG AGGAGCTGAT AGGTTATTTG 
ATCGACGAGG AAATAATTGA AGGAATGAGG AGCAGGCAAG CAGCATTTGA CGAGGGCCGC 
ACGATGCTTG ATAAACTTGA AAAAGTCTGT TTATTGGAAA GAGCTTGTTA TGGTGATCAT 
AGTACAACTG TCAAGATGCA TGACTTGATT AGGGACATGG CCCACCAAAT ACTCCAAACA 
AACTCTCCAG TCATGGTTGG GGGATATAAT GATAAATTAC CAGATGTGGA TATGTGGAAA 
GAGAATCTAG TGAGAGTCTC TCTGAAGCAT TGTTATTTCG AGGAAATTCC TTCTAGTCAT 
TCACCAAGAT GTCCCAATCT TTCAACTCTA TTGCTGTGTG ATAATCCTTA TTTGCAATTT 
ATTGCAGATT CTTTTTTCAC ACAATTGCAT GGGCTCAAGG TTCTTGATCT GTCTCGTACA 
GAAATCATAG AATTGCCTGA TTCTGTCTCT GAACTGGTGA GTCTCACTGC ATTATTGCTC 
AAACAATGTG AATATTTAAT ACATGTACCA TCATTAGAAA AGCTCACAGC ACTGAGGAGG 
CTAGATCTCT CTGGTACTTG GGAACTTGAA AAGATACCTC AAGACATGCA ATGTCTATCT 
AACCTGAGGT ATCTGAGGAT GGATGGATGT GGTGTAAAGG AGTTTCCTAC TGGGATATTA 
CCAAAACTCT TTCACCTACA ACTCTTCATG TTAGAGGGGA AGACTAATTA TGACTACGTT 
CAAGTAACAG TTAAGGGAAA GGAAGTAGGA TGCTTGAGGG AGTTGGAAAA TTTGGTATGC 
AATTTTGAAG GTCAATCTGA CTTTGTGGAG TATCTCAATT CTCGGGATAA GACCCGATCA 
CTAAGTACAT ACGATATTTT TGTAGGACCA CTGGATGAAG ATTTTTATAG TGAAATGAAG 
CGTGGTTATG GCAAAAGTAA AACAGTTTGG TTGAGTAGTT TGTGTAACAA TGGAGATGGA 
AATTTTCAAG TAATGTTCCC AAATGACATT CAAAAACTGA TTATTTTTAA TTGTTCATGT 
GATGTTTCCT CTTTAATAGA GCATTCAATT AAACTGGAGG TCATCCACAT TAAGGATTGC 
AATAGCATGG AGAGCTTGAT TTCATCTTCT TTGTTCTGCC CTTCTCCAAC ATCATTGCCA 
TCTTATAATG GTGTATTTTC TACTCTTAAA GAGTTTAATT GTTATGGATG TAGTAGTATG 
AAGAAGTTGT TCCCTCTTGT CTTGCTGCCA AACCTCGTAA ACCTAGTAAA TATTACAGTT 
ATAGATTGTG CAAAAATGGA GGAGATAATA GGTGGAAGAA GATCAGATGA AGAAAACAGC 
AGCAACAACA CCGAATTCAA GCTCCCAAAG TTAAGATCTC TGGCATTGAG TTGGTTACCA 
AAACTGAAAA GAATTTGTAG TGCAAAACTG ATTTGCGATT CTCTCCAACA AATTGAAGTA 
AGTTACTGCA ATAGCATGGA GAGCTTGGTT CCATCTTCTT GGATTTCCCT CGTAAACCTA 
GAAAAGATTA CAGTTAGAGG ATGTCAGAAG ATGGAGGAGA TAATAGGTGG AAGAAGATCA 
GATGAAGAAA GCAGCAGCAA CAACACCGAA TTCAAACTCC CAAAGTTAAG ATCTCTGGCA 
TTGGAAGATT TACCAGAACT GAAAAGCATT TGTAGTGCAA AGCTGACGTG CGATTCTCTC 
CAACAAATTG AAGTATGGAA TTGCAACAGC ATGGAGAGCT TGGTTCCATC TTCTTGGATT 
TCCCTCGTAA ACCTAGAAAA GATTACAGTT AGAGGAGGTG AGAAAATGGA GGAGATAATA 
GGTGGAAGAA GATCAGATGA AGAAAGCAGC AGCACCGAAT TCAAACTCCC AAAGTTAAGA 
TCTCTGGCAT TGTTTAATTT ACCAGAACTG AAAAGCATTT GTAGTGCAAA GCTGACGTGC 
GATTCTCTCC AACAAATTGA AGTATGGAAT TGCAACAGCA TGGAGATCTT GGTTCCATCT 
TCTTGGATTT CCCTTGTAAA CCTAGAAAAG ATTACAGTTA GTGCATGTAA GAAGATGAAG 
GAGATAATAG GTGGAACAAG ATCAGATGAA GAAAGTAGCA GTAACAACAC CGAATTCAAA 
CTCCCAAAGT TAAGATCTCT GGCATTGAGT TGGTTACCAG AACTGAAAAG AATTTGTAGT 
GCAAAACTGA TTTGCGATTC TCTCAGAATG ATTGAAGTTT ATAAATGTCA GAAGCTGAAG 
AGGATGCCAT TGTGGAAGAA GGATTATACA TCCAAGAGAA TGGTGGGAGT CAGTAGTGGT 
GTGGGAGCAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024447687.1
CDS	857..4951
Translation	MSRPNDPFWEYAEKMDDGSMTCTFCGHCFSQGTSVTRIKLHLAGVKRRGVKICKNVPEEVRDAACDAVNNSPPEKKLKSVAGSSSNEVANAISASTQEQNNEVTHVEMAQQGGPFFTGELAWANDLIGEAELVQLERGSSHERPSINQTDEPRGDSSRPTDDQLCSPSVNNDANMNDVQNMVAVRIEPVPVQVLEQSNAEVDSLAGHAGRIQVGVHGMEQGAEEERICPNSAANGMENTGDGSSQHSRRLMIDAHDNTGEEATQRIDLVQRLEGETWGQMNAISAFLMGEEDVENNRGRLVQPGAACSSGGAACNTNKIKGDALPTRKMVGQAFEEHKKTISSLLMRNEVSSIGIYGMGGVGKTTLGTHIHNQLLERPETPVYWITVSHNTSIPRLQTSLAGRIGLDLSKVDEELHRAVALKKELMKKQKWILILDDLWKAFDLQKLGVPDQVEEGCKLILTSRSAKVCQQMKTQHTIKVQPISEKEAWTLFIERLGHDIAFSSEVEGIALNVVRECAGLPLGIITIAASMRGVDEPHEWRNTLKKLKESKYKEMEDEVFRLLRFSYDQLNDLALQQCLLYCALYPEDHRIEREELIGYLIDEEIIEGMRSRQAAFDEGRTMLDKLEKVCLLERACYGDHSTTVKMHDLIRDMAHQILQTNSPVMVGGYNDKLPDVDMWKENLVRVSLKHCYFEEIPSSHSPRCPNLSTLLLCDNPYLQFIADSFFTQLHGLKVLDLSRTEIIELPDSVSELVSLTALLLKQCEYLIHVPSLEKLTALRRLDLSGTWELEKIPQDMQCLSNLRYLRMDGCGVKEFPTGILPKLFHLQLFMLEGKTNYDYVQVTVKGKEVGCLRELENLVCNFEGQSDFVEYLNSRDKTRSLSTYDIFVGPLDEDFYSEMKRGYGKSKTVWLSSLCNNGDGNFQVMFPNDIQKLIIFNCSCDVSSLIEHSIKLEVIHIKDCNSMESLISSSLFCPSPTSLPSYNGVFSTLKEFNCYGCSSMKKLFPLVLLPNLVNLVNITVIDCAKMEEIIGGRRSDEENSSNNTEFKLPKLRSLALSWLPKLKRICSAKLICDSLQQIEVSYCNSMESLVPSSWISLVNLEKITVRGCQKMEEIIGGRRSDEESSSNNTEFKLPKLRSLALEDLPELKSICSAKLTCDSLQQIEVWNCNSMESLVPSSWISLVNLEKITVRGGEKMEEIIGGRRSDEESSSTEFKLPKLRSLALFNLPELKSICSAKLTCDSLQQIEVWNCNSMEILVPSSWISLVNLEKITVSACKKMKEIIGGTRSDEESSSNNTEFKLPKLRSLALSWLPELKRICSAKLICDSLRMIEVYKCQKLKRMPLWKKDYTSKRMVGVSSGVGAS

Target ORF information:

RefSeq Version	XM_024591919.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024591919.1

ORF Insert Sequence:

ATGAGTCGAC CAAATGATCC TTTTTGGGAA TATGCTGAAA AGATGGATGA TGGTAGCATG 
ACGTGTACGT TTTGTGGGCA TTGTTTTTCC CAAGGTACTT CCGTTACGAG GATCAAATTG 
CATTTGGCAG GAGTGAAACG GCGTGGTGTT AAAATTTGTA AAAATGTTCC AGAAGAAGTT 
CGAGATGCAG CTTGTGATGC AGTTAATAAT AGCCCTCCAG AAAAGAAACT TAAATCTGTT 
GCGGGCTCAA GCAGTAACGA GGTCGCTAAT GCAATTTCAG CTTCAACGCA AGAACAGAAC 
AATGAAGTGA CCCATGTAGA GATGGCACAA CAAGGAGGAC CTTTTTTCAC TGGAGAACTT 
GCATGGGCAA ATGATTTGAT TGGAGAGGCT GAACTTGTGC AGCTGGAGAG AGGTAGTTCT 
CATGAGAGGC CATCAATTAA TCAAACAGAT GAGCCTCGAG GAGATTCATC CCGACCAACA 
GATGATCAAC TCTGTTCTCC ATCAGTAAAC AATGATGCCA ATATGAACGA TGTGCAGAAC 
ATGGTTGCAG TCAGGATAGA ACCAGTACCG GTTCAAGTGT TGGAACAAAG CAATGCAGAA 
GTTGACAGTT TGGCAGGGCA TGCTGGAAGG ATACAAGTGG GAGTTCATGG CATGGAACAA 
GGTGCGGAGG AAGAGAGAAT TTGTCCGAAC TCAGCGGCAA ATGGCATGGA AAACACTGGT 
GATGGATCTA GTCAGCATTC CAGGAGACTC ATGATTGATG CACATGATAA TACAGGGGAA 
GAAGCTACAC AAAGAATAGA TCTAGTGCAA CGATTAGAAG GGGAAACTTG GGGTCAGATG 
AATGCAATCT CTGCTTTTTT AATGGGGGAG GAGGACGTGG AGAACAATCG TGGAAGATTA 
GTGCAGCCTG GAGCAGCTTG CTCTTCTGGA GGGGCTGCAT GCAACACAAA TAAGATTAAA 
GGAGATGCAC TGCCAACGAG AAAAATGGTG GGTCAAGCAT TCGAAGAACA TAAGAAGACT 
ATCTCATCTT TGTTGATGCG CAATGAAGTT TCAAGTATTG GTATCTATGG GATGGGCGGA 
GTGGGAAAGA CCACATTGGG GACACATATA CATAATCAGC TTCTAGAAAG ACCAGAGACT 
CCTGTTTACT GGATTACTGT TTCACATAAT ACCAGCATTC CTAGATTGCA GACAAGTCTC 
GCAGGACGAA TTGGTTTAGA TCTTTCCAAG GTAGATGAGG AGCTGCATAG AGCGGTAGCG 
TTGAAAAAAG AATTAATGAA GAAACAGAAA TGGATTCTGA TTTTAGATGA TTTGTGGAAG 
GCTTTTGACC TGCAAAAGCT GGGAGTTCCT GACCAAGTTG AGGAGGGATG CAAGCTGATT 
CTTACATCTC GATCAGCAAA GGTTTGTCAA CAGATGAAAA CCCAACACAC GATAAAAGTG 
CAGCCTATTT CGGAGAAAGA AGCTTGGACT TTGTTTATTG AGAGACTTGG ACATGACATA 
GCATTTTCTT CAGAAGTGGA AGGAATTGCT TTGAATGTTG TAAGGGAATG TGCTGGTTTG 
CCATTGGGAA TTATTACAAT AGCAGCAAGC ATGAGAGGAG TGGATGAGCC ACATGAGTGG 
AGGAATACAT TGAAGAAATT GAAAGAATCA AAATATAAGG AAATGGAAGA TGAGGTATTC 
CGATTATTGA GGTTTAGTTA TGATCAGCTA AATGATTTAG CATTACAACA ATGTCTCTTG 
TACTGTGCAT TGTATCCTGA AGATCATAGG ATTGAAAGGG AGGAGCTGAT AGGTTATTTG 
ATCGACGAGG AAATAATTGA AGGAATGAGG AGCAGGCAAG CAGCATTTGA CGAGGGCCGC 
ACGATGCTTG ATAAACTTGA AAAAGTCTGT TTATTGGAAA GAGCTTGTTA TGGTGATCAT 
AGTACAACTG TCAAGATGCA TGACTTGATT AGGGACATGG CCCACCAAAT ACTCCAAACA 
AACTCTCCAG TCATGGTTGG GGGATATAAT GATAAATTAC CAGATGTGGA TATGTGGAAA 
GAGAATCTAG TGAGAGTCTC TCTGAAGCAT TGTTATTTCG AGGAAATTCC TTCTAGTCAT 
TCACCAAGAT GTCCCAATCT TTCAACTCTA TTGCTGTGTG ATAATCCTTA TTTGCAATTT 
ATTGCAGATT CTTTTTTCAC ACAATTGCAT GGGCTCAAGG TTCTTGATCT GTCTCGTACA 
GAAATCATAG AATTGCCTGA TTCTGTCTCT GAACTGGTGA GTCTCACTGC ATTATTGCTC 
AAACAATGTG AATATTTAAT ACATGTACCA TCATTAGAAA AGCTCACAGC ACTGAGGAGG 
CTAGATCTCT CTGGTACTTG GGAACTTGAA AAGATACCTC AAGACATGCA ATGTCTATCT 
AACCTGAGGT ATCTGAGGAT GGATGGATGT GGTGTAAAGG AGTTTCCTAC TGGGATATTA 
CCAAAACTCT TTCACCTACA ACTCTTCATG TTAGAGGGGA AGACTAATTA TGACTACGTT 
CAAGTAACAG TTAAGGGAAA GGAAGTAGGA TGCTTGAGGG AGTTGGAAAA TTTGGTATGC 
AATTTTGAAG GTCAATCTGA CTTTGTGGAG TATCTCAATT CTCGGGATAA GACCCGATCA 
CTAAGTACAT ACGATATTTT TGTAGGACCA CTGGATGAAG ATTTTTATAG TGAAATGAAG 
CGTGGTTATG GCAAAAGTAA AACAGTTTGG TTGAGTAGTT TGTGTAACAA TGGAGATGGA 
AATTTTCAAG TAATGTTCCC AAATGACATT CAAAAACTGA TTATTTTTAA TTGTTCATGT 
GATGTTTCCT CTTTAATAGA GCATTCAATT AAACTGGAGG TCATCCACAT TAAGGATTGC 
AATAGCATGG AGAGCTTGAT TTCATCTTCT TTGTTCTGCC CTTCTCCAAC ATCATTGCCA 
TCTTATAATG GTGTATTTTC TACTCTTAAA GAGTTTAATT GTTATGGATG TAGTAGTATG 
AAGAAGTTGT TCCCTCTTGT CTTGCTGCCA AACCTCGTAA ACCTAGTAAA TATTACAGTT 
ATAGATTGTG CAAAAATGGA GGAGATAATA GGTGGAAGAA GATCAGATGA AGAAAACAGC 
AGCAACAACA CCGAATTCAA GCTCCCAAAG TTAAGATCTC TGGCATTGAG TTGGTTACCA 
AAACTGAAAA GAATTTGTAG TGCAAAACTG ATTTGCGATT CTCTCCAACA AATTGAAGTA 
AGTTACTGCA ATAGCATGGA GAGCTTGGTT CCATCTTCTT GGATTTCCCT CGTAAACCTA 
GAAAAGATTA CAGTTAGAGG ATGTCAGAAG ATGGAGGAGA TAATAGGTGG AAGAAGATCA 
GATGAAGAAA GCAGCAGCAA CAACACCGAA TTCAAACTCC CAAAGTTAAG ATCTCTGGCA 
TTGGAAGATT TACCAGAACT GAAAAGCATT TGTAGTGCAA AGCTGACGTG CGATTCTCTC 
CAACAAATTG AAGTATGGAA TTGCAACAGC ATGGAGAGCT TGGTTCCATC TTCTTGGATT 
TCCCTCGTAA ACCTAGAAAA GATTACAGTT AGAGGAGGTG AGAAAATGGA GGAGATAATA 
GGTGGAAGAA GATCAGATGA AGAAAGCAGC AGCACCGAAT TCAAACTCCC AAAGTTAAGA 
TCTCTGGCAT TGTTTAATTT ACCAGAACTG AAAAGCATTT GTAGTGCAAA GCTGACGTGC 
GATTCTCTCC AACAAATTGA AGTATGGAAT TGCAACAGCA TGGAGATCTT GGTTCCATCT 
TCTTGGATTT CCCTTGTAAA CCTAGAAAAG ATTACAGTTA GTGCATGTAA GAAGATGAAG 
GAGATAATAG GTGGAACAAG ATCAGATGAA GAAAGTAGCA GTAACAACAC CGAATTCAAA 
CTCCCAAAGT TAAGATCTCT GGCATTGAGT TGGTTACCAG AACTGAAAAG AATTTGTAGT 
GCAAAACTGA TTTGCGATTC TCTCAGAATG ATTGAAGTTT ATAAATGTCA GAAGCTGAAG 
AGGATGCCAT TGTGGAAGAA GGATTATACA TCCAAGAGAA TGGTGGGAGT CAGTAGTGGT 
GTGGGAGCAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18107958 ( XM_024591910.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024447678.1 Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X1, mRNA.

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CloneID	OPa418670
Clone ID Related Accession (Same CDS sequence)	XM_024591920.1 , XM_024591911.1 , XM_024591912.1 , XM_024591917.1 , XM_024591913.1 , XM_024591916.1 , XM_024591918.1 , XM_024591919.1 , XM_024591910.1 , XM_024591914.1
Accession Version	XM_024591910.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	probable disease resistance protein At4g27220 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020167869.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCGAC CAAATGATCC TTTTTGGGAA TATGCTGAAA AGATGGATGA TGGTAGCATG 
ACGTGTACGT TTTGTGGGCA TTGTTTTTCC CAAGGTACTT CCGTTACGAG GATCAAATTG 
CATTTGGCAG GAGTGAAACG GCGTGGTGTT AAAATTTGTA AAAATGTTCC AGAAGAAGTT 
CGAGATGCAG CTTGTGATGC AGTTAATAAT AGCCCTCCAG AAAAGAAACT TAAATCTGTT 
GCGGGCTCAA GCAGTAACGA GGTCGCTAAT GCAATTTCAG CTTCAACGCA AGAACAGAAC 
AATGAAGTGA CCCATGTAGA GATGGCACAA CAAGGAGGAC CTTTTTTCAC TGGAGAACTT 
GCATGGGCAA ATGATTTGAT TGGAGAGGCT GAACTTGTGC AGCTGGAGAG AGGTAGTTCT 
CATGAGAGGC CATCAATTAA TCAAACAGAT GAGCCTCGAG GAGATTCATC CCGACCAACA 
GATGATCAAC TCTGTTCTCC ATCAGTAAAC AATGATGCCA ATATGAACGA TGTGCAGAAC 
ATGGTTGCAG TCAGGATAGA ACCAGTACCG GTTCAAGTGT TGGAACAAAG CAATGCAGAA 
GTTGACAGTT TGGCAGGGCA TGCTGGAAGG ATACAAGTGG GAGTTCATGG CATGGAACAA 
GGTGCGGAGG AAGAGAGAAT TTGTCCGAAC TCAGCGGCAA ATGGCATGGA AAACACTGGT 
GATGGATCTA GTCAGCATTC CAGGAGACTC ATGATTGATG CACATGATAA TACAGGGGAA 
GAAGCTACAC AAAGAATAGA TCTAGTGCAA CGATTAGAAG GGGAAACTTG GGGTCAGATG 
AATGCAATCT CTGCTTTTTT AATGGGGGAG GAGGACGTGG AGAACAATCG TGGAAGATTA 
GTGCAGCCTG GAGCAGCTTG CTCTTCTGGA GGGGCTGCAT GCAACACAAA TAAGATTAAA 
GGAGATGCAC TGCCAACGAG AAAAATGGTG GGTCAAGCAT TCGAAGAACA TAAGAAGACT 
ATCTCATCTT TGTTGATGCG CAATGAAGTT TCAAGTATTG GTATCTATGG GATGGGCGGA 
GTGGGAAAGA CCACATTGGG GACACATATA CATAATCAGC TTCTAGAAAG ACCAGAGACT 
CCTGTTTACT GGATTACTGT TTCACATAAT ACCAGCATTC CTAGATTGCA GACAAGTCTC 
GCAGGACGAA TTGGTTTAGA TCTTTCCAAG GTAGATGAGG AGCTGCATAG AGCGGTAGCG 
TTGAAAAAAG AATTAATGAA GAAACAGAAA TGGATTCTGA TTTTAGATGA TTTGTGGAAG 
GCTTTTGACC TGCAAAAGCT GGGAGTTCCT GACCAAGTTG AGGAGGGATG CAAGCTGATT 
CTTACATCTC GATCAGCAAA GGTTTGTCAA CAGATGAAAA CCCAACACAC GATAAAAGTG 
CAGCCTATTT CGGAGAAAGA AGCTTGGACT TTGTTTATTG AGAGACTTGG ACATGACATA 
GCATTTTCTT CAGAAGTGGA AGGAATTGCT TTGAATGTTG TAAGGGAATG TGCTGGTTTG 
CCATTGGGAA TTATTACAAT AGCAGCAAGC ATGAGAGGAG TGGATGAGCC ACATGAGTGG 
AGGAATACAT TGAAGAAATT GAAAGAATCA AAATATAAGG AAATGGAAGA TGAGGTATTC 
CGATTATTGA GGTTTAGTTA TGATCAGCTA AATGATTTAG CATTACAACA ATGTCTCTTG 
TACTGTGCAT TGTATCCTGA AGATCATAGG ATTGAAAGGG AGGAGCTGAT AGGTTATTTG 
ATCGACGAGG AAATAATTGA AGGAATGAGG AGCAGGCAAG CAGCATTTGA CGAGGGCCGC 
ACGATGCTTG ATAAACTTGA AAAAGTCTGT TTATTGGAAA GAGCTTGTTA TGGTGATCAT 
AGTACAACTG TCAAGATGCA TGACTTGATT AGGGACATGG CCCACCAAAT ACTCCAAACA 
AACTCTCCAG TCATGGTTGG GGGATATAAT GATAAATTAC CAGATGTGGA TATGTGGAAA 
GAGAATCTAG TGAGAGTCTC TCTGAAGCAT TGTTATTTCG AGGAAATTCC TTCTAGTCAT 
TCACCAAGAT GTCCCAATCT TTCAACTCTA TTGCTGTGTG ATAATCCTTA TTTGCAATTT 
ATTGCAGATT CTTTTTTCAC ACAATTGCAT GGGCTCAAGG TTCTTGATCT GTCTCGTACA 
GAAATCATAG AATTGCCTGA TTCTGTCTCT GAACTGGTGA GTCTCACTGC ATTATTGCTC 
AAACAATGTG AATATTTAAT ACATGTACCA TCATTAGAAA AGCTCACAGC ACTGAGGAGG 
CTAGATCTCT CTGGTACTTG GGAACTTGAA AAGATACCTC AAGACATGCA ATGTCTATCT 
AACCTGAGGT ATCTGAGGAT GGATGGATGT GGTGTAAAGG AGTTTCCTAC TGGGATATTA 
CCAAAACTCT TTCACCTACA ACTCTTCATG TTAGAGGGGA AGACTAATTA TGACTACGTT 
CAAGTAACAG TTAAGGGAAA GGAAGTAGGA TGCTTGAGGG AGTTGGAAAA TTTGGTATGC 
AATTTTGAAG GTCAATCTGA CTTTGTGGAG TATCTCAATT CTCGGGATAA GACCCGATCA 
CTAAGTACAT ACGATATTTT TGTAGGACCA CTGGATGAAG ATTTTTATAG TGAAATGAAG 
CGTGGTTATG GCAAAAGTAA AACAGTTTGG TTGAGTAGTT TGTGTAACAA TGGAGATGGA 
AATTTTCAAG TAATGTTCCC AAATGACATT CAAAAACTGA TTATTTTTAA TTGTTCATGT 
GATGTTTCCT CTTTAATAGA GCATTCAATT AAACTGGAGG TCATCCACAT TAAGGATTGC 
AATAGCATGG AGAGCTTGAT TTCATCTTCT TTGTTCTGCC CTTCTCCAAC ATCATTGCCA 
TCTTATAATG GTGTATTTTC TACTCTTAAA GAGTTTAATT GTTATGGATG TAGTAGTATG 
AAGAAGTTGT TCCCTCTTGT CTTGCTGCCA AACCTCGTAA ACCTAGTAAA TATTACAGTT 
ATAGATTGTG CAAAAATGGA GGAGATAATA GGTGGAAGAA GATCAGATGA AGAAAACAGC 
AGCAACAACA CCGAATTCAA GCTCCCAAAG TTAAGATCTC TGGCATTGAG TTGGTTACCA 
AAACTGAAAA GAATTTGTAG TGCAAAACTG ATTTGCGATT CTCTCCAACA AATTGAAGTA 
AGTTACTGCA ATAGCATGGA GAGCTTGGTT CCATCTTCTT GGATTTCCCT CGTAAACCTA 
GAAAAGATTA CAGTTAGAGG ATGTCAGAAG ATGGAGGAGA TAATAGGTGG AAGAAGATCA 
GATGAAGAAA GCAGCAGCAA CAACACCGAA TTCAAACTCC CAAAGTTAAG ATCTCTGGCA 
TTGGAAGATT TACCAGAACT GAAAAGCATT TGTAGTGCAA AGCTGACGTG CGATTCTCTC 
CAACAAATTG AAGTATGGAA TTGCAACAGC ATGGAGAGCT TGGTTCCATC TTCTTGGATT 
TCCCTCGTAA ACCTAGAAAA GATTACAGTT AGAGGAGGTG AGAAAATGGA GGAGATAATA 
GGTGGAAGAA GATCAGATGA AGAAAGCAGC AGCACCGAAT TCAAACTCCC AAAGTTAAGA 
TCTCTGGCAT TGTTTAATTT ACCAGAACTG AAAAGCATTT GTAGTGCAAA GCTGACGTGC 
GATTCTCTCC AACAAATTGA AGTATGGAAT TGCAACAGCA TGGAGATCTT GGTTCCATCT 
TCTTGGATTT CCCTTGTAAA CCTAGAAAAG ATTACAGTTA GTGCATGTAA GAAGATGAAG 
GAGATAATAG GTGGAACAAG ATCAGATGAA GAAAGTAGCA GTAACAACAC CGAATTCAAA 
CTCCCAAAGT TAAGATCTCT GGCATTGAGT TGGTTACCAG AACTGAAAAG AATTTGTAGT 
GCAAAACTGA TTTGCGATTC TCTCAGAATG ATTGAAGTTT ATAAATGTCA GAAGCTGAAG 
AGGATGCCAT TGTGGAAGAA GGATTATACA TCCAAGAGAA TGGTGGGAGT CAGTAGTGGT 
GTGGGAGCAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024447678.1
CDS	901..4995
Translation	MSRPNDPFWEYAEKMDDGSMTCTFCGHCFSQGTSVTRIKLHLAGVKRRGVKICKNVPEEVRDAACDAVNNSPPEKKLKSVAGSSSNEVANAISASTQEQNNEVTHVEMAQQGGPFFTGELAWANDLIGEAELVQLERGSSHERPSINQTDEPRGDSSRPTDDQLCSPSVNNDANMNDVQNMVAVRIEPVPVQVLEQSNAEVDSLAGHAGRIQVGVHGMEQGAEEERICPNSAANGMENTGDGSSQHSRRLMIDAHDNTGEEATQRIDLVQRLEGETWGQMNAISAFLMGEEDVENNRGRLVQPGAACSSGGAACNTNKIKGDALPTRKMVGQAFEEHKKTISSLLMRNEVSSIGIYGMGGVGKTTLGTHIHNQLLERPETPVYWITVSHNTSIPRLQTSLAGRIGLDLSKVDEELHRAVALKKELMKKQKWILILDDLWKAFDLQKLGVPDQVEEGCKLILTSRSAKVCQQMKTQHTIKVQPISEKEAWTLFIERLGHDIAFSSEVEGIALNVVRECAGLPLGIITIAASMRGVDEPHEWRNTLKKLKESKYKEMEDEVFRLLRFSYDQLNDLALQQCLLYCALYPEDHRIEREELIGYLIDEEIIEGMRSRQAAFDEGRTMLDKLEKVCLLERACYGDHSTTVKMHDLIRDMAHQILQTNSPVMVGGYNDKLPDVDMWKENLVRVSLKHCYFEEIPSSHSPRCPNLSTLLLCDNPYLQFIADSFFTQLHGLKVLDLSRTEIIELPDSVSELVSLTALLLKQCEYLIHVPSLEKLTALRRLDLSGTWELEKIPQDMQCLSNLRYLRMDGCGVKEFPTGILPKLFHLQLFMLEGKTNYDYVQVTVKGKEVGCLRELENLVCNFEGQSDFVEYLNSRDKTRSLSTYDIFVGPLDEDFYSEMKRGYGKSKTVWLSSLCNNGDGNFQVMFPNDIQKLIIFNCSCDVSSLIEHSIKLEVIHIKDCNSMESLISSSLFCPSPTSLPSYNGVFSTLKEFNCYGCSSMKKLFPLVLLPNLVNLVNITVIDCAKMEEIIGGRRSDEENSSNNTEFKLPKLRSLALSWLPKLKRICSAKLICDSLQQIEVSYCNSMESLVPSSWISLVNLEKITVRGCQKMEEIIGGRRSDEESSSNNTEFKLPKLRSLALEDLPELKSICSAKLTCDSLQQIEVWNCNSMESLVPSSWISLVNLEKITVRGGEKMEEIIGGRRSDEESSSTEFKLPKLRSLALFNLPELKSICSAKLTCDSLQQIEVWNCNSMEILVPSSWISLVNLEKITVSACKKMKEIIGGTRSDEESSSNNTEFKLPKLRSLALSWLPELKRICSAKLICDSLRMIEVYKCQKLKRMPLWKKDYTSKRMVGVSSGVGAS

Target ORF information:

RefSeq Version	XM_024591910.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024591910.1

ORF Insert Sequence:

ATGAGTCGAC CAAATGATCC TTTTTGGGAA TATGCTGAAA AGATGGATGA TGGTAGCATG 
ACGTGTACGT TTTGTGGGCA TTGTTTTTCC CAAGGTACTT CCGTTACGAG GATCAAATTG 
CATTTGGCAG GAGTGAAACG GCGTGGTGTT AAAATTTGTA AAAATGTTCC AGAAGAAGTT 
CGAGATGCAG CTTGTGATGC AGTTAATAAT AGCCCTCCAG AAAAGAAACT TAAATCTGTT 
GCGGGCTCAA GCAGTAACGA GGTCGCTAAT GCAATTTCAG CTTCAACGCA AGAACAGAAC 
AATGAAGTGA CCCATGTAGA GATGGCACAA CAAGGAGGAC CTTTTTTCAC TGGAGAACTT 
GCATGGGCAA ATGATTTGAT TGGAGAGGCT GAACTTGTGC AGCTGGAGAG AGGTAGTTCT 
CATGAGAGGC CATCAATTAA TCAAACAGAT GAGCCTCGAG GAGATTCATC CCGACCAACA 
GATGATCAAC TCTGTTCTCC ATCAGTAAAC AATGATGCCA ATATGAACGA TGTGCAGAAC 
ATGGTTGCAG TCAGGATAGA ACCAGTACCG GTTCAAGTGT TGGAACAAAG CAATGCAGAA 
GTTGACAGTT TGGCAGGGCA TGCTGGAAGG ATACAAGTGG GAGTTCATGG CATGGAACAA 
GGTGCGGAGG AAGAGAGAAT TTGTCCGAAC TCAGCGGCAA ATGGCATGGA AAACACTGGT 
GATGGATCTA GTCAGCATTC CAGGAGACTC ATGATTGATG CACATGATAA TACAGGGGAA 
GAAGCTACAC AAAGAATAGA TCTAGTGCAA CGATTAGAAG GGGAAACTTG GGGTCAGATG 
AATGCAATCT CTGCTTTTTT AATGGGGGAG GAGGACGTGG AGAACAATCG TGGAAGATTA 
GTGCAGCCTG GAGCAGCTTG CTCTTCTGGA GGGGCTGCAT GCAACACAAA TAAGATTAAA 
GGAGATGCAC TGCCAACGAG AAAAATGGTG GGTCAAGCAT TCGAAGAACA TAAGAAGACT 
ATCTCATCTT TGTTGATGCG CAATGAAGTT TCAAGTATTG GTATCTATGG GATGGGCGGA 
GTGGGAAAGA CCACATTGGG GACACATATA CATAATCAGC TTCTAGAAAG ACCAGAGACT 
CCTGTTTACT GGATTACTGT TTCACATAAT ACCAGCATTC CTAGATTGCA GACAAGTCTC 
GCAGGACGAA TTGGTTTAGA TCTTTCCAAG GTAGATGAGG AGCTGCATAG AGCGGTAGCG 
TTGAAAAAAG AATTAATGAA GAAACAGAAA TGGATTCTGA TTTTAGATGA TTTGTGGAAG 
GCTTTTGACC TGCAAAAGCT GGGAGTTCCT GACCAAGTTG AGGAGGGATG CAAGCTGATT 
CTTACATCTC GATCAGCAAA GGTTTGTCAA CAGATGAAAA CCCAACACAC GATAAAAGTG 
CAGCCTATTT CGGAGAAAGA AGCTTGGACT TTGTTTATTG AGAGACTTGG ACATGACATA 
GCATTTTCTT CAGAAGTGGA AGGAATTGCT TTGAATGTTG TAAGGGAATG TGCTGGTTTG 
CCATTGGGAA TTATTACAAT AGCAGCAAGC ATGAGAGGAG TGGATGAGCC ACATGAGTGG 
AGGAATACAT TGAAGAAATT GAAAGAATCA AAATATAAGG AAATGGAAGA TGAGGTATTC 
CGATTATTGA GGTTTAGTTA TGATCAGCTA AATGATTTAG CATTACAACA ATGTCTCTTG 
TACTGTGCAT TGTATCCTGA AGATCATAGG ATTGAAAGGG AGGAGCTGAT AGGTTATTTG 
ATCGACGAGG AAATAATTGA AGGAATGAGG AGCAGGCAAG CAGCATTTGA CGAGGGCCGC 
ACGATGCTTG ATAAACTTGA AAAAGTCTGT TTATTGGAAA GAGCTTGTTA TGGTGATCAT 
AGTACAACTG TCAAGATGCA TGACTTGATT AGGGACATGG CCCACCAAAT ACTCCAAACA 
AACTCTCCAG TCATGGTTGG GGGATATAAT GATAAATTAC CAGATGTGGA TATGTGGAAA 
GAGAATCTAG TGAGAGTCTC TCTGAAGCAT TGTTATTTCG AGGAAATTCC TTCTAGTCAT 
TCACCAAGAT GTCCCAATCT TTCAACTCTA TTGCTGTGTG ATAATCCTTA TTTGCAATTT 
ATTGCAGATT CTTTTTTCAC ACAATTGCAT GGGCTCAAGG TTCTTGATCT GTCTCGTACA 
GAAATCATAG AATTGCCTGA TTCTGTCTCT GAACTGGTGA GTCTCACTGC ATTATTGCTC 
AAACAATGTG AATATTTAAT ACATGTACCA TCATTAGAAA AGCTCACAGC ACTGAGGAGG 
CTAGATCTCT CTGGTACTTG GGAACTTGAA AAGATACCTC AAGACATGCA ATGTCTATCT 
AACCTGAGGT ATCTGAGGAT GGATGGATGT GGTGTAAAGG AGTTTCCTAC TGGGATATTA 
CCAAAACTCT TTCACCTACA ACTCTTCATG TTAGAGGGGA AGACTAATTA TGACTACGTT 
CAAGTAACAG TTAAGGGAAA GGAAGTAGGA TGCTTGAGGG AGTTGGAAAA TTTGGTATGC 
AATTTTGAAG GTCAATCTGA CTTTGTGGAG TATCTCAATT CTCGGGATAA GACCCGATCA 
CTAAGTACAT ACGATATTTT TGTAGGACCA CTGGATGAAG ATTTTTATAG TGAAATGAAG 
CGTGGTTATG GCAAAAGTAA AACAGTTTGG TTGAGTAGTT TGTGTAACAA TGGAGATGGA 
AATTTTCAAG TAATGTTCCC AAATGACATT CAAAAACTGA TTATTTTTAA TTGTTCATGT 
GATGTTTCCT CTTTAATAGA GCATTCAATT AAACTGGAGG TCATCCACAT TAAGGATTGC 
AATAGCATGG AGAGCTTGAT TTCATCTTCT TTGTTCTGCC CTTCTCCAAC ATCATTGCCA 
TCTTATAATG GTGTATTTTC TACTCTTAAA GAGTTTAATT GTTATGGATG TAGTAGTATG 
AAGAAGTTGT TCCCTCTTGT CTTGCTGCCA AACCTCGTAA ACCTAGTAAA TATTACAGTT 
ATAGATTGTG CAAAAATGGA GGAGATAATA GGTGGAAGAA GATCAGATGA AGAAAACAGC 
AGCAACAACA CCGAATTCAA GCTCCCAAAG TTAAGATCTC TGGCATTGAG TTGGTTACCA 
AAACTGAAAA GAATTTGTAG TGCAAAACTG ATTTGCGATT CTCTCCAACA AATTGAAGTA 
AGTTACTGCA ATAGCATGGA GAGCTTGGTT CCATCTTCTT GGATTTCCCT CGTAAACCTA 
GAAAAGATTA CAGTTAGAGG ATGTCAGAAG ATGGAGGAGA TAATAGGTGG AAGAAGATCA 
GATGAAGAAA GCAGCAGCAA CAACACCGAA TTCAAACTCC CAAAGTTAAG ATCTCTGGCA 
TTGGAAGATT TACCAGAACT GAAAAGCATT TGTAGTGCAA AGCTGACGTG CGATTCTCTC 
CAACAAATTG AAGTATGGAA TTGCAACAGC ATGGAGAGCT TGGTTCCATC TTCTTGGATT 
TCCCTCGTAA ACCTAGAAAA GATTACAGTT AGAGGAGGTG AGAAAATGGA GGAGATAATA 
GGTGGAAGAA GATCAGATGA AGAAAGCAGC AGCACCGAAT TCAAACTCCC AAAGTTAAGA 
TCTCTGGCAT TGTTTAATTT ACCAGAACTG AAAAGCATTT GTAGTGCAAA GCTGACGTGC 
GATTCTCTCC AACAAATTGA AGTATGGAAT TGCAACAGCA TGGAGATCTT GGTTCCATCT 
TCTTGGATTT CCCTTGTAAA CCTAGAAAAG ATTACAGTTA GTGCATGTAA GAAGATGAAG 
GAGATAATAG GTGGAACAAG ATCAGATGAA GAAAGTAGCA GTAACAACAC CGAATTCAAA 
CTCCCAAAGT TAAGATCTCT GGCATTGAGT TGGTTACCAG AACTGAAAAG AATTTGTAGT 
GCAAAACTGA TTTGCGATTC TCTCAGAATG ATTGAAGTTT ATAAATGTCA GAAGCTGAAG 
AGGATGCCAT TGTGGAAGAA GGATTATACA TCCAAGAGAA TGGTGGGAGT CAGTAGTGGT 
GTGGGAGCAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18107958 ( XM_024591914.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024447682.1 Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X5, mRNA.

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CloneID	OPa418670
Clone ID Related Accession (Same CDS sequence)	XM_024591920.1 , XM_024591911.1 , XM_024591912.1 , XM_024591917.1 , XM_024591913.1 , XM_024591916.1 , XM_024591918.1 , XM_024591919.1 , XM_024591910.1 , XM_024591914.1
Accession Version	XM_024591914.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	probable disease resistance protein At4g27220 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020167869.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCGAC CAAATGATCC TTTTTGGGAA TATGCTGAAA AGATGGATGA TGGTAGCATG 
ACGTGTACGT TTTGTGGGCA TTGTTTTTCC CAAGGTACTT CCGTTACGAG GATCAAATTG 
CATTTGGCAG GAGTGAAACG GCGTGGTGTT AAAATTTGTA AAAATGTTCC AGAAGAAGTT 
CGAGATGCAG CTTGTGATGC AGTTAATAAT AGCCCTCCAG AAAAGAAACT TAAATCTGTT 
GCGGGCTCAA GCAGTAACGA GGTCGCTAAT GCAATTTCAG CTTCAACGCA AGAACAGAAC 
AATGAAGTGA CCCATGTAGA GATGGCACAA CAAGGAGGAC CTTTTTTCAC TGGAGAACTT 
GCATGGGCAA ATGATTTGAT TGGAGAGGCT GAACTTGTGC AGCTGGAGAG AGGTAGTTCT 
CATGAGAGGC CATCAATTAA TCAAACAGAT GAGCCTCGAG GAGATTCATC CCGACCAACA 
GATGATCAAC TCTGTTCTCC ATCAGTAAAC AATGATGCCA ATATGAACGA TGTGCAGAAC 
ATGGTTGCAG TCAGGATAGA ACCAGTACCG GTTCAAGTGT TGGAACAAAG CAATGCAGAA 
GTTGACAGTT TGGCAGGGCA TGCTGGAAGG ATACAAGTGG GAGTTCATGG CATGGAACAA 
GGTGCGGAGG AAGAGAGAAT TTGTCCGAAC TCAGCGGCAA ATGGCATGGA AAACACTGGT 
GATGGATCTA GTCAGCATTC CAGGAGACTC ATGATTGATG CACATGATAA TACAGGGGAA 
GAAGCTACAC AAAGAATAGA TCTAGTGCAA CGATTAGAAG GGGAAACTTG GGGTCAGATG 
AATGCAATCT CTGCTTTTTT AATGGGGGAG GAGGACGTGG AGAACAATCG TGGAAGATTA 
GTGCAGCCTG GAGCAGCTTG CTCTTCTGGA GGGGCTGCAT GCAACACAAA TAAGATTAAA 
GGAGATGCAC TGCCAACGAG AAAAATGGTG GGTCAAGCAT TCGAAGAACA TAAGAAGACT 
ATCTCATCTT TGTTGATGCG CAATGAAGTT TCAAGTATTG GTATCTATGG GATGGGCGGA 
GTGGGAAAGA CCACATTGGG GACACATATA CATAATCAGC TTCTAGAAAG ACCAGAGACT 
CCTGTTTACT GGATTACTGT TTCACATAAT ACCAGCATTC CTAGATTGCA GACAAGTCTC 
GCAGGACGAA TTGGTTTAGA TCTTTCCAAG GTAGATGAGG AGCTGCATAG AGCGGTAGCG 
TTGAAAAAAG AATTAATGAA GAAACAGAAA TGGATTCTGA TTTTAGATGA TTTGTGGAAG 
GCTTTTGACC TGCAAAAGCT GGGAGTTCCT GACCAAGTTG AGGAGGGATG CAAGCTGATT 
CTTACATCTC GATCAGCAAA GGTTTGTCAA CAGATGAAAA CCCAACACAC GATAAAAGTG 
CAGCCTATTT CGGAGAAAGA AGCTTGGACT TTGTTTATTG AGAGACTTGG ACATGACATA 
GCATTTTCTT CAGAAGTGGA AGGAATTGCT TTGAATGTTG TAAGGGAATG TGCTGGTTTG 
CCATTGGGAA TTATTACAAT AGCAGCAAGC ATGAGAGGAG TGGATGAGCC ACATGAGTGG 
AGGAATACAT TGAAGAAATT GAAAGAATCA AAATATAAGG AAATGGAAGA TGAGGTATTC 
CGATTATTGA GGTTTAGTTA TGATCAGCTA AATGATTTAG CATTACAACA ATGTCTCTTG 
TACTGTGCAT TGTATCCTGA AGATCATAGG ATTGAAAGGG AGGAGCTGAT AGGTTATTTG 
ATCGACGAGG AAATAATTGA AGGAATGAGG AGCAGGCAAG CAGCATTTGA CGAGGGCCGC 
ACGATGCTTG ATAAACTTGA AAAAGTCTGT TTATTGGAAA GAGCTTGTTA TGGTGATCAT 
AGTACAACTG TCAAGATGCA TGACTTGATT AGGGACATGG CCCACCAAAT ACTCCAAACA 
AACTCTCCAG TCATGGTTGG GGGATATAAT GATAAATTAC CAGATGTGGA TATGTGGAAA 
GAGAATCTAG TGAGAGTCTC TCTGAAGCAT TGTTATTTCG AGGAAATTCC TTCTAGTCAT 
TCACCAAGAT GTCCCAATCT TTCAACTCTA TTGCTGTGTG ATAATCCTTA TTTGCAATTT 
ATTGCAGATT CTTTTTTCAC ACAATTGCAT GGGCTCAAGG TTCTTGATCT GTCTCGTACA 
GAAATCATAG AATTGCCTGA TTCTGTCTCT GAACTGGTGA GTCTCACTGC ATTATTGCTC 
AAACAATGTG AATATTTAAT ACATGTACCA TCATTAGAAA AGCTCACAGC ACTGAGGAGG 
CTAGATCTCT CTGGTACTTG GGAACTTGAA AAGATACCTC AAGACATGCA ATGTCTATCT 
AACCTGAGGT ATCTGAGGAT GGATGGATGT GGTGTAAAGG AGTTTCCTAC TGGGATATTA 
CCAAAACTCT TTCACCTACA ACTCTTCATG TTAGAGGGGA AGACTAATTA TGACTACGTT 
CAAGTAACAG TTAAGGGAAA GGAAGTAGGA TGCTTGAGGG AGTTGGAAAA TTTGGTATGC 
AATTTTGAAG GTCAATCTGA CTTTGTGGAG TATCTCAATT CTCGGGATAA GACCCGATCA 
CTAAGTACAT ACGATATTTT TGTAGGACCA CTGGATGAAG ATTTTTATAG TGAAATGAAG 
CGTGGTTATG GCAAAAGTAA AACAGTTTGG TTGAGTAGTT TGTGTAACAA TGGAGATGGA 
AATTTTCAAG TAATGTTCCC AAATGACATT CAAAAACTGA TTATTTTTAA TTGTTCATGT 
GATGTTTCCT CTTTAATAGA GCATTCAATT AAACTGGAGG TCATCCACAT TAAGGATTGC 
AATAGCATGG AGAGCTTGAT TTCATCTTCT TTGTTCTGCC CTTCTCCAAC ATCATTGCCA 
TCTTATAATG GTGTATTTTC TACTCTTAAA GAGTTTAATT GTTATGGATG TAGTAGTATG 
AAGAAGTTGT TCCCTCTTGT CTTGCTGCCA AACCTCGTAA ACCTAGTAAA TATTACAGTT 
ATAGATTGTG CAAAAATGGA GGAGATAATA GGTGGAAGAA GATCAGATGA AGAAAACAGC 
AGCAACAACA CCGAATTCAA GCTCCCAAAG TTAAGATCTC TGGCATTGAG TTGGTTACCA 
AAACTGAAAA GAATTTGTAG TGCAAAACTG ATTTGCGATT CTCTCCAACA AATTGAAGTA 
AGTTACTGCA ATAGCATGGA GAGCTTGGTT CCATCTTCTT GGATTTCCCT CGTAAACCTA 
GAAAAGATTA CAGTTAGAGG ATGTCAGAAG ATGGAGGAGA TAATAGGTGG AAGAAGATCA 
GATGAAGAAA GCAGCAGCAA CAACACCGAA TTCAAACTCC CAAAGTTAAG ATCTCTGGCA 
TTGGAAGATT TACCAGAACT GAAAAGCATT TGTAGTGCAA AGCTGACGTG CGATTCTCTC 
CAACAAATTG AAGTATGGAA TTGCAACAGC ATGGAGAGCT TGGTTCCATC TTCTTGGATT 
TCCCTCGTAA ACCTAGAAAA GATTACAGTT AGAGGAGGTG AGAAAATGGA GGAGATAATA 
GGTGGAAGAA GATCAGATGA AGAAAGCAGC AGCACCGAAT TCAAACTCCC AAAGTTAAGA 
TCTCTGGCAT TGTTTAATTT ACCAGAACTG AAAAGCATTT GTAGTGCAAA GCTGACGTGC 
GATTCTCTCC AACAAATTGA AGTATGGAAT TGCAACAGCA TGGAGATCTT GGTTCCATCT 
TCTTGGATTT CCCTTGTAAA CCTAGAAAAG ATTACAGTTA GTGCATGTAA GAAGATGAAG 
GAGATAATAG GTGGAACAAG ATCAGATGAA GAAAGTAGCA GTAACAACAC CGAATTCAAA 
CTCCCAAAGT TAAGATCTCT GGCATTGAGT TGGTTACCAG AACTGAAAAG AATTTGTAGT 
GCAAAACTGA TTTGCGATTC TCTCAGAATG ATTGAAGTTT ATAAATGTCA GAAGCTGAAG 
AGGATGCCAT TGTGGAAGAA GGATTATACA TCCAAGAGAA TGGTGGGAGT CAGTAGTGGT 
GTGGGAGCAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024447682.1
CDS	901..4995
Translation	MSRPNDPFWEYAEKMDDGSMTCTFCGHCFSQGTSVTRIKLHLAGVKRRGVKICKNVPEEVRDAACDAVNNSPPEKKLKSVAGSSSNEVANAISASTQEQNNEVTHVEMAQQGGPFFTGELAWANDLIGEAELVQLERGSSHERPSINQTDEPRGDSSRPTDDQLCSPSVNNDANMNDVQNMVAVRIEPVPVQVLEQSNAEVDSLAGHAGRIQVGVHGMEQGAEEERICPNSAANGMENTGDGSSQHSRRLMIDAHDNTGEEATQRIDLVQRLEGETWGQMNAISAFLMGEEDVENNRGRLVQPGAACSSGGAACNTNKIKGDALPTRKMVGQAFEEHKKTISSLLMRNEVSSIGIYGMGGVGKTTLGTHIHNQLLERPETPVYWITVSHNTSIPRLQTSLAGRIGLDLSKVDEELHRAVALKKELMKKQKWILILDDLWKAFDLQKLGVPDQVEEGCKLILTSRSAKVCQQMKTQHTIKVQPISEKEAWTLFIERLGHDIAFSSEVEGIALNVVRECAGLPLGIITIAASMRGVDEPHEWRNTLKKLKESKYKEMEDEVFRLLRFSYDQLNDLALQQCLLYCALYPEDHRIEREELIGYLIDEEIIEGMRSRQAAFDEGRTMLDKLEKVCLLERACYGDHSTTVKMHDLIRDMAHQILQTNSPVMVGGYNDKLPDVDMWKENLVRVSLKHCYFEEIPSSHSPRCPNLSTLLLCDNPYLQFIADSFFTQLHGLKVLDLSRTEIIELPDSVSELVSLTALLLKQCEYLIHVPSLEKLTALRRLDLSGTWELEKIPQDMQCLSNLRYLRMDGCGVKEFPTGILPKLFHLQLFMLEGKTNYDYVQVTVKGKEVGCLRELENLVCNFEGQSDFVEYLNSRDKTRSLSTYDIFVGPLDEDFYSEMKRGYGKSKTVWLSSLCNNGDGNFQVMFPNDIQKLIIFNCSCDVSSLIEHSIKLEVIHIKDCNSMESLISSSLFCPSPTSLPSYNGVFSTLKEFNCYGCSSMKKLFPLVLLPNLVNLVNITVIDCAKMEEIIGGRRSDEENSSNNTEFKLPKLRSLALSWLPKLKRICSAKLICDSLQQIEVSYCNSMESLVPSSWISLVNLEKITVRGCQKMEEIIGGRRSDEESSSNNTEFKLPKLRSLALEDLPELKSICSAKLTCDSLQQIEVWNCNSMESLVPSSWISLVNLEKITVRGGEKMEEIIGGRRSDEESSSTEFKLPKLRSLALFNLPELKSICSAKLTCDSLQQIEVWNCNSMEILVPSSWISLVNLEKITVSACKKMKEIIGGTRSDEESSSNNTEFKLPKLRSLALSWLPELKRICSAKLICDSLRMIEVYKCQKLKRMPLWKKDYTSKRMVGVSSGVGAS

Target ORF information:

RefSeq Version	XM_024591914.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024591914.1

ORF Insert Sequence:

ATGAGTCGAC CAAATGATCC TTTTTGGGAA TATGCTGAAA AGATGGATGA TGGTAGCATG 
ACGTGTACGT TTTGTGGGCA TTGTTTTTCC CAAGGTACTT CCGTTACGAG GATCAAATTG 
CATTTGGCAG GAGTGAAACG GCGTGGTGTT AAAATTTGTA AAAATGTTCC AGAAGAAGTT 
CGAGATGCAG CTTGTGATGC AGTTAATAAT AGCCCTCCAG AAAAGAAACT TAAATCTGTT 
GCGGGCTCAA GCAGTAACGA GGTCGCTAAT GCAATTTCAG CTTCAACGCA AGAACAGAAC 
AATGAAGTGA CCCATGTAGA GATGGCACAA CAAGGAGGAC CTTTTTTCAC TGGAGAACTT 
GCATGGGCAA ATGATTTGAT TGGAGAGGCT GAACTTGTGC AGCTGGAGAG AGGTAGTTCT 
CATGAGAGGC CATCAATTAA TCAAACAGAT GAGCCTCGAG GAGATTCATC CCGACCAACA 
GATGATCAAC TCTGTTCTCC ATCAGTAAAC AATGATGCCA ATATGAACGA TGTGCAGAAC 
ATGGTTGCAG TCAGGATAGA ACCAGTACCG GTTCAAGTGT TGGAACAAAG CAATGCAGAA 
GTTGACAGTT TGGCAGGGCA TGCTGGAAGG ATACAAGTGG GAGTTCATGG CATGGAACAA 
GGTGCGGAGG AAGAGAGAAT TTGTCCGAAC TCAGCGGCAA ATGGCATGGA AAACACTGGT 
GATGGATCTA GTCAGCATTC CAGGAGACTC ATGATTGATG CACATGATAA TACAGGGGAA 
GAAGCTACAC AAAGAATAGA TCTAGTGCAA CGATTAGAAG GGGAAACTTG GGGTCAGATG 
AATGCAATCT CTGCTTTTTT AATGGGGGAG GAGGACGTGG AGAACAATCG TGGAAGATTA 
GTGCAGCCTG GAGCAGCTTG CTCTTCTGGA GGGGCTGCAT GCAACACAAA TAAGATTAAA 
GGAGATGCAC TGCCAACGAG AAAAATGGTG GGTCAAGCAT TCGAAGAACA TAAGAAGACT 
ATCTCATCTT TGTTGATGCG CAATGAAGTT TCAAGTATTG GTATCTATGG GATGGGCGGA 
GTGGGAAAGA CCACATTGGG GACACATATA CATAATCAGC TTCTAGAAAG ACCAGAGACT 
CCTGTTTACT GGATTACTGT TTCACATAAT ACCAGCATTC CTAGATTGCA GACAAGTCTC 
GCAGGACGAA TTGGTTTAGA TCTTTCCAAG GTAGATGAGG AGCTGCATAG AGCGGTAGCG 
TTGAAAAAAG AATTAATGAA GAAACAGAAA TGGATTCTGA TTTTAGATGA TTTGTGGAAG 
GCTTTTGACC TGCAAAAGCT GGGAGTTCCT GACCAAGTTG AGGAGGGATG CAAGCTGATT 
CTTACATCTC GATCAGCAAA GGTTTGTCAA CAGATGAAAA CCCAACACAC GATAAAAGTG 
CAGCCTATTT CGGAGAAAGA AGCTTGGACT TTGTTTATTG AGAGACTTGG ACATGACATA 
GCATTTTCTT CAGAAGTGGA AGGAATTGCT TTGAATGTTG TAAGGGAATG TGCTGGTTTG 
CCATTGGGAA TTATTACAAT AGCAGCAAGC ATGAGAGGAG TGGATGAGCC ACATGAGTGG 
AGGAATACAT TGAAGAAATT GAAAGAATCA AAATATAAGG AAATGGAAGA TGAGGTATTC 
CGATTATTGA GGTTTAGTTA TGATCAGCTA AATGATTTAG CATTACAACA ATGTCTCTTG 
TACTGTGCAT TGTATCCTGA AGATCATAGG ATTGAAAGGG AGGAGCTGAT AGGTTATTTG 
ATCGACGAGG AAATAATTGA AGGAATGAGG AGCAGGCAAG CAGCATTTGA CGAGGGCCGC 
ACGATGCTTG ATAAACTTGA AAAAGTCTGT TTATTGGAAA GAGCTTGTTA TGGTGATCAT 
AGTACAACTG TCAAGATGCA TGACTTGATT AGGGACATGG CCCACCAAAT ACTCCAAACA 
AACTCTCCAG TCATGGTTGG GGGATATAAT GATAAATTAC CAGATGTGGA TATGTGGAAA 
GAGAATCTAG TGAGAGTCTC TCTGAAGCAT TGTTATTTCG AGGAAATTCC TTCTAGTCAT 
TCACCAAGAT GTCCCAATCT TTCAACTCTA TTGCTGTGTG ATAATCCTTA TTTGCAATTT 
ATTGCAGATT CTTTTTTCAC ACAATTGCAT GGGCTCAAGG TTCTTGATCT GTCTCGTACA 
GAAATCATAG AATTGCCTGA TTCTGTCTCT GAACTGGTGA GTCTCACTGC ATTATTGCTC 
AAACAATGTG AATATTTAAT ACATGTACCA TCATTAGAAA AGCTCACAGC ACTGAGGAGG 
CTAGATCTCT CTGGTACTTG GGAACTTGAA AAGATACCTC AAGACATGCA ATGTCTATCT 
AACCTGAGGT ATCTGAGGAT GGATGGATGT GGTGTAAAGG AGTTTCCTAC TGGGATATTA 
CCAAAACTCT TTCACCTACA ACTCTTCATG TTAGAGGGGA AGACTAATTA TGACTACGTT 
CAAGTAACAG TTAAGGGAAA GGAAGTAGGA TGCTTGAGGG AGTTGGAAAA TTTGGTATGC 
AATTTTGAAG GTCAATCTGA CTTTGTGGAG TATCTCAATT CTCGGGATAA GACCCGATCA 
CTAAGTACAT ACGATATTTT TGTAGGACCA CTGGATGAAG ATTTTTATAG TGAAATGAAG 
CGTGGTTATG GCAAAAGTAA AACAGTTTGG TTGAGTAGTT TGTGTAACAA TGGAGATGGA 
AATTTTCAAG TAATGTTCCC AAATGACATT CAAAAACTGA TTATTTTTAA TTGTTCATGT 
GATGTTTCCT CTTTAATAGA GCATTCAATT AAACTGGAGG TCATCCACAT TAAGGATTGC 
AATAGCATGG AGAGCTTGAT TTCATCTTCT TTGTTCTGCC CTTCTCCAAC ATCATTGCCA 
TCTTATAATG GTGTATTTTC TACTCTTAAA GAGTTTAATT GTTATGGATG TAGTAGTATG 
AAGAAGTTGT TCCCTCTTGT CTTGCTGCCA AACCTCGTAA ACCTAGTAAA TATTACAGTT 
ATAGATTGTG CAAAAATGGA GGAGATAATA GGTGGAAGAA GATCAGATGA AGAAAACAGC 
AGCAACAACA CCGAATTCAA GCTCCCAAAG TTAAGATCTC TGGCATTGAG TTGGTTACCA 
AAACTGAAAA GAATTTGTAG TGCAAAACTG ATTTGCGATT CTCTCCAACA AATTGAAGTA 
AGTTACTGCA ATAGCATGGA GAGCTTGGTT CCATCTTCTT GGATTTCCCT CGTAAACCTA 
GAAAAGATTA CAGTTAGAGG ATGTCAGAAG ATGGAGGAGA TAATAGGTGG AAGAAGATCA 
GATGAAGAAA GCAGCAGCAA CAACACCGAA TTCAAACTCC CAAAGTTAAG ATCTCTGGCA 
TTGGAAGATT TACCAGAACT GAAAAGCATT TGTAGTGCAA AGCTGACGTG CGATTCTCTC 
CAACAAATTG AAGTATGGAA TTGCAACAGC ATGGAGAGCT TGGTTCCATC TTCTTGGATT 
TCCCTCGTAA ACCTAGAAAA GATTACAGTT AGAGGAGGTG AGAAAATGGA GGAGATAATA 
GGTGGAAGAA GATCAGATGA AGAAAGCAGC AGCACCGAAT TCAAACTCCC AAAGTTAAGA 
TCTCTGGCAT TGTTTAATTT ACCAGAACTG AAAAGCATTT GTAGTGCAAA GCTGACGTGC 
GATTCTCTCC AACAAATTGA AGTATGGAAT TGCAACAGCA TGGAGATCTT GGTTCCATCT 
TCTTGGATTT CCCTTGTAAA CCTAGAAAAG ATTACAGTTA GTGCATGTAA GAAGATGAAG 
GAGATAATAG GTGGAACAAG ATCAGATGAA GAAAGTAGCA GTAACAACAC CGAATTCAAA 
CTCCCAAAGT TAAGATCTCT GGCATTGAGT TGGTTACCAG AACTGAAAAG AATTTGTAGT 
GCAAAACTGA TTTGCGATTC TCTCAGAATG ATTGAAGTTT ATAAATGTCA GAAGCTGAAG 
AGGATGCCAT TGTGGAAGAA GGATTATACA TCCAAGAGAA TGGTGGGAGT CAGTAGTGGT 
GTGGGAGCAT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18107958 ( XM_024591922.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024447690.1 Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OPa418671
Clone ID Related Accession (Same CDS sequence)	XM_024591922.1
Accession Version	XM_024591922.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	probable disease resistance protein At4g27220 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020167869.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCGAT CAAATGATCC ATTTTGGAAT GATGTTGAAG ATATGAATAA TGGTGGCATG 
AAGTGTAAAT TTTGTGGGCA TTTATTTTCC GAGAATACTT CCATTTCGAG GATCAAATGG 
CATTTGTCAG GAGTGAAAGG GTGGGGTGTT AAAATATGTG AGAAAGTTCC TGAAGAAGTT 
CAAAATGTAG TCCGTGCAGC AATGGATGGC CCTCCAGAAA AAAAACTTAA AACCGTAGCA 
GGCTCAAGCA ATAACAAGGT CACTATTGCA GTTTTAGCTT CTGCCCAAGA ACAAAACAAT 
GATGAAGTGA TGGCACAAGA AGGAGATGCT CTTTTCGTTG GAGAGCGCGA ACATTGGCTG 
AGTAGCTTCA CTGATGAAGA CATTGAGCTT CTGAGAGGTA TCTTTCATGA GAGAACATCA 
ATTAATCAAG CTGATGAGCC TCGAGGAGAT CCATCCCAGC CAACAGATCC ATTGTGTCTT 
GACCATGGAA GATATTATGA TCGACCCCGT GCTCCATTAG TAAACAATGA TGTAATTCTG 
AATCTTGTGG AGGACATGGT TAGAGTGAGG ACAGAACCAG TGGAGGAGAA GGAAGAGAAG 
AAGGATGTGG AGAAGAATAG TGGAAGATTA GTGCAGCCTG GCGCAGCAGC TAGCTCTTCT 
GGAGGGTTTG CAAGCAACAC AAATGAGATT AAAGGAGATG CACTACCAAC GAGAAAGCTG 
GTGGGTCAAG CATTCGAAGA CCACAAGAAG ACTATCTGGT CTTGGTTAAT GCATGATAAA 
GTTTCAAGAA TTAGCATCTA TGGGATGGGC GGAGTGGGTA AGACGACGTT GGTGACACAC 
ATATATAATC AGCTTCTAGA AAGACCAGAC ACTTTCAATC ATGTTTTCTG GATTACTGTT 
TCACAAGATA CCAGCATAAA CAGTTTGCAG AAAAGTTTCG CAAGATGTAT TGGTTTAGAT 
CTTTCTAATG AAGATGAGGA GCTGCACAGA GCGGTCATAT TGAGAAAAGA ATTAATGAAG 
AAAAAGAAAT GGGTTTTGAT TTTAGATGAT CTGTGGAAGG CTTTTGACTT ACAAAAGCTG 
GGAATTCCTG ACCAAGTTAA GGGATGCAAG TTGATTATTA CAACTCGATC AGGAAGAATT 
TGTCAACAGA TGAAAACCCA ACACACAATC AAAGTGCAGC CTATTTTGAA GGAAGAAGCT 
TGGATTTTGT TTATTGAGAG GCTTGGAAAT GACATAGCAC TTTCTCCAGA AGTGGAACGA 
ATTGCAGTAG ATGTTGCAAG GGAATGTGCC GGTTTGCCAT TGGGAATTAT TACAATGGCA 
GGAAGCATGA GGGGAGTGGA TGACCTACAT GAGTGGAGAA ATACATTGAA GAAATTGAAA 
GAATCAAAAT TTAGAGACAT GGAAGATGAG GTATTCCAGT TGTTGAGGTT GAGTTATGAT 
CGGCTAGAAA ATGATTTAGC ACTACAACAA TGTCTCTTGT ATTGTGCATT ATATCCTGAA 
GATCATAAGA TTGAAAGGGA GGAGTTGATA TGTTATTTGA TTGATGAGGG AATAGTTGAA 
GGAACGAGTA GCAGACAAGC AGCATTTGAC GAGGGCCACA CGATGCTTAA TAAACTTGAA 
AATGTTTGTT TACTGGAAAG TTGTTTTGAT CGTTATCATC ATAGATGTGT CAAGATGCAC 
GACTTGATTA GGGACATGGC CCTCCAAATA CTCCAAATGA ACTCTCCAGT TATGGTCAGG 
GAATATGAGG AAGAAATACC AGATGTGGAT ATGTGGAAAG AAGATCTAGT GAGAGTCTCT 
TTGAAGGATT GTTATTTCAA GGAAATTCCA TCCAGCCATT CACCAAGGTG TCCCAATCTG 
TCAACTCTGT TGTTACGCAA TAATGAAGGG TTGGAATTTA TTGCAGCTTC TTTTTTTGAG 
CAATTGCACG GACTCAAGGT TCTTAATCTG TCTCATACAA ATATCATAAA TTTGCCTGAT 
TCTGTTTCTG AATTGGTGAG TCTTACTGCA TTATTGCTCA AAGGGTGTGT GAACTTAAGG 
CTTGTACCAT CATTAAAAAA GCTCAAGGCA CTGAAGAGGT TAGACCTCTC TCGTACTGCA 
CTTGAAAAGA TGCCTCAAGG CATGGAATGT GTATCCAACC TGAGATATCT TAGGATGAAT 
GGATTTGGGG AAAAGGAGTT TCCTAATGGG ATATTACCTA AACTCTCTCA CCTACAAGTC 
TTTGTATTAG AGGATGCGTT TCTTACTTGT CCGATAATAG TCAAAGGAAA GGAAGTAGGA 
TGCTTGAAGG AGTTGGAAAC TGTGGTATGC CATTTTGAAG GTCAATCTGA CTTTGTGGAG 
TATCTTAATT CTCGGGACAA GACCCAATCA CTAAGCACAT ACCAAATCTT TGTAGGACTG 
CTGGATGAAG ATGATTATAG TAGAATGATG GATGATTATC ACACAAGTAA AACAATTGCC 
TTGTGTAATT TGAGTATCAA TAGGGATGGA GATTTTCAGG TCATGTTCCC AAATGACATT 
CAAGAACTGG TTATTTATAA ATGTTCATGT GATGTTTCCT CTCTAATAAA GTATGCAACT 
GATCTGGAGG TTATCAAGAT ATGGGATAGC AATAGCATCG AGAGCTTGGT TTCATCTTCT 
TGGTTCTGCT CTGCTCCCTT ACCATTGCCA TCTTATAATG GTATATTTTC TGGTCTTAAA 
GAGTTTAATT GCTATGGATG TAGAAGTATG AAGAAGTTGT TCCCTATTCT CTTGCTACCA 
TACCTCGTAA ACTTGAAAGT GATTAGAGTT TTTTATTGTG AGAAGATGGA GGAGATAGTA 
GGGACAAGAT CAGAAGAAGA AGCGGTTATG GTTGAAGAAA GCAGCAGTAG CAGCACCGAA 
TTCAAACTCC CAAAGTTAAG AAATCTGGAA TTGAGGGGAT TACCAGAACT GAAAAGCATT 
TGTAGTGCAA AACTGGTCTG TGATTCTCTC CAACATATTT GGATAGCAAA TTGTCAGAAG 
TTGAAGAGAA TGCCAATTTG TCTTCCGTTG CTTGAAAATG GCCAGCCATC TGCTCTCCCT 
TCTCTTCAAA ATATCTTTAT ATATCCAAGA GAATGGTGGG AGTCTGTAGT GGAGTGGGAT 
CATCCTAATG CTAAGAACGT CCTTCGTCCC TTCGTAAAGT TTTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024447690.1
CDS	165..3332
Translation	MVRSNDPFWNDVEDMNNGGMKCKFCGHLFSENTSISRIKWHLSGVKGWGVKICEKVPEEVQNVVRAAMDGPPEKKLKTVAGSSNNKVTIAVLASAQEQNNDEVMAQEGDALFVGEREHWLSSFTDEDIELLRGIFHERTSINQADEPRGDPSQPTDPLCLDHGRYYDRPRAPLVNNDVILNLVEDMVRVRTEPVEEKEEKKDVEKNSGRLVQPGAAASSSGGFASNTNEIKGDALPTRKLVGQAFEDHKKTIWSWLMHDKVSRISIYGMGGVGKTTLVTHIYNQLLERPDTFNHVFWITVSQDTSINSLQKSFARCIGLDLSNEDEELHRAVILRKELMKKKKWVLILDDLWKAFDLQKLGIPDQVKGCKLIITTRSGRICQQMKTQHTIKVQPILKEEAWILFIERLGNDIALSPEVERIAVDVARECAGLPLGIITMAGSMRGVDDLHEWRNTLKKLKESKFRDMEDEVFQLLRLSYDRLENDLALQQCLLYCALYPEDHKIEREELICYLIDEGIVEGTSSRQAAFDEGHTMLNKLENVCLLESCFDRYHHRCVKMHDLIRDMALQILQMNSPVMVREYEEEIPDVDMWKEDLVRVSLKDCYFKEIPSSHSPRCPNLSTLLLRNNEGLEFIAASFFEQLHGLKVLNLSHTNIINLPDSVSELVSLTALLLKGCVNLRLVPSLKKLKALKRLDLSRTALEKMPQGMECVSNLRYLRMNGFGEKEFPNGILPKLSHLQVFVLEDAFLTCPIIVKGKEVGCLKELETVVCHFEGQSDFVEYLNSRDKTQSLSTYQIFVGLLDEDDYSRMMDDYHTSKTIALCNLSINRDGDFQVMFPNDIQELVIYKCSCDVSSLIKYATDLEVIKIWDSNSIESLVSSSWFCSAPLPLPSYNGIFSGLKEFNCYGCRSMKKLFPILLLPYLVNLKVIRVFYCEKMEEIVGTRSEEEAVMVEESSSSSTEFKLPKLRNLELRGLPELKSICSAKLVCDSLQHIWIANCQKLKRMPICLPLLENGQPSALPSLQNIFIYPREWWESVVEWDHPNAKNVLRPFVKFL

Target ORF information:

RefSeq Version	XM_024591922.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa probable disease resistance protein At4g27220 (LOC18107958), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024591922.1

ORF Insert Sequence:

ATGGTTCGAT CAAATGATCC ATTTTGGAAT GATGTTGAAG ATATGAATAA TGGTGGCATG 
AAGTGTAAAT TTTGTGGGCA TTTATTTTCC GAGAATACTT CCATTTCGAG GATCAAATGG 
CATTTGTCAG GAGTGAAAGG GTGGGGTGTT AAAATATGTG AGAAAGTTCC TGAAGAAGTT 
CAAAATGTAG TCCGTGCAGC AATGGATGGC CCTCCAGAAA AAAAACTTAA AACCGTAGCA 
GGCTCAAGCA ATAACAAGGT CACTATTGCA GTTTTAGCTT CTGCCCAAGA ACAAAACAAT 
GATGAAGTGA TGGCACAAGA AGGAGATGCT CTTTTCGTTG GAGAGCGCGA ACATTGGCTG 
AGTAGCTTCA CTGATGAAGA CATTGAGCTT CTGAGAGGTA TCTTTCATGA GAGAACATCA 
ATTAATCAAG CTGATGAGCC TCGAGGAGAT CCATCCCAGC CAACAGATCC ATTGTGTCTT 
GACCATGGAA GATATTATGA TCGACCCCGT GCTCCATTAG TAAACAATGA TGTAATTCTG 
AATCTTGTGG AGGACATGGT TAGAGTGAGG ACAGAACCAG TGGAGGAGAA GGAAGAGAAG 
AAGGATGTGG AGAAGAATAG TGGAAGATTA GTGCAGCCTG GCGCAGCAGC TAGCTCTTCT 
GGAGGGTTTG CAAGCAACAC AAATGAGATT AAAGGAGATG CACTACCAAC GAGAAAGCTG 
GTGGGTCAAG CATTCGAAGA CCACAAGAAG ACTATCTGGT CTTGGTTAAT GCATGATAAA 
GTTTCAAGAA TTAGCATCTA TGGGATGGGC GGAGTGGGTA AGACGACGTT GGTGACACAC 
ATATATAATC AGCTTCTAGA AAGACCAGAC ACTTTCAATC ATGTTTTCTG GATTACTGTT 
TCACAAGATA CCAGCATAAA CAGTTTGCAG AAAAGTTTCG CAAGATGTAT TGGTTTAGAT 
CTTTCTAATG AAGATGAGGA GCTGCACAGA GCGGTCATAT TGAGAAAAGA ATTAATGAAG 
AAAAAGAAAT GGGTTTTGAT TTTAGATGAT CTGTGGAAGG CTTTTGACTT ACAAAAGCTG 
GGAATTCCTG ACCAAGTTAA GGGATGCAAG TTGATTATTA CAACTCGATC AGGAAGAATT 
TGTCAACAGA TGAAAACCCA ACACACAATC AAAGTGCAGC CTATTTTGAA GGAAGAAGCT 
TGGATTTTGT TTATTGAGAG GCTTGGAAAT GACATAGCAC TTTCTCCAGA AGTGGAACGA 
ATTGCAGTAG ATGTTGCAAG GGAATGTGCC GGTTTGCCAT TGGGAATTAT TACAATGGCA 
GGAAGCATGA GGGGAGTGGA TGACCTACAT GAGTGGAGAA ATACATTGAA GAAATTGAAA 
GAATCAAAAT TTAGAGACAT GGAAGATGAG GTATTCCAGT TGTTGAGGTT GAGTTATGAT 
CGGCTAGAAA ATGATTTAGC ACTACAACAA TGTCTCTTGT ATTGTGCATT ATATCCTGAA 
GATCATAAGA TTGAAAGGGA GGAGTTGATA TGTTATTTGA TTGATGAGGG AATAGTTGAA 
GGAACGAGTA GCAGACAAGC AGCATTTGAC GAGGGCCACA CGATGCTTAA TAAACTTGAA 
AATGTTTGTT TACTGGAAAG TTGTTTTGAT CGTTATCATC ATAGATGTGT CAAGATGCAC 
GACTTGATTA GGGACATGGC CCTCCAAATA CTCCAAATGA ACTCTCCAGT TATGGTCAGG 
GAATATGAGG AAGAAATACC AGATGTGGAT ATGTGGAAAG AAGATCTAGT GAGAGTCTCT 
TTGAAGGATT GTTATTTCAA GGAAATTCCA TCCAGCCATT CACCAAGGTG TCCCAATCTG 
TCAACTCTGT TGTTACGCAA TAATGAAGGG TTGGAATTTA TTGCAGCTTC TTTTTTTGAG 
CAATTGCACG GACTCAAGGT TCTTAATCTG TCTCATACAA ATATCATAAA TTTGCCTGAT 
TCTGTTTCTG AATTGGTGAG TCTTACTGCA TTATTGCTCA AAGGGTGTGT GAACTTAAGG 
CTTGTACCAT CATTAAAAAA GCTCAAGGCA CTGAAGAGGT TAGACCTCTC TCGTACTGCA 
CTTGAAAAGA TGCCTCAAGG CATGGAATGT GTATCCAACC TGAGATATCT TAGGATGAAT 
GGATTTGGGG AAAAGGAGTT TCCTAATGGG ATATTACCTA AACTCTCTCA CCTACAAGTC 
TTTGTATTAG AGGATGCGTT TCTTACTTGT CCGATAATAG TCAAAGGAAA GGAAGTAGGA 
TGCTTGAAGG AGTTGGAAAC TGTGGTATGC CATTTTGAAG GTCAATCTGA CTTTGTGGAG 
TATCTTAATT CTCGGGACAA GACCCAATCA CTAAGCACAT ACCAAATCTT TGTAGGACTG 
CTGGATGAAG ATGATTATAG TAGAATGATG GATGATTATC ACACAAGTAA AACAATTGCC 
TTGTGTAATT TGAGTATCAA TAGGGATGGA GATTTTCAGG TCATGTTCCC AAATGACATT 
CAAGAACTGG TTATTTATAA ATGTTCATGT GATGTTTCCT CTCTAATAAA GTATGCAACT 
GATCTGGAGG TTATCAAGAT ATGGGATAGC AATAGCATCG AGAGCTTGGT TTCATCTTCT 
TGGTTCTGCT CTGCTCCCTT ACCATTGCCA TCTTATAATG GTATATTTTC TGGTCTTAAA 
GAGTTTAATT GCTATGGATG TAGAAGTATG AAGAAGTTGT TCCCTATTCT CTTGCTACCA 
TACCTCGTAA ACTTGAAAGT GATTAGAGTT TTTTATTGTG AGAAGATGGA GGAGATAGTA 
GGGACAAGAT CAGAAGAAGA AGCGGTTATG GTTGAAGAAA GCAGCAGTAG CAGCACCGAA 
TTCAAACTCC CAAAGTTAAG AAATCTGGAA TTGAGGGGAT TACCAGAACT GAAAAGCATT 
TGTAGTGCAA AACTGGTCTG TGATTCTCTC CAACATATTT GGATAGCAAA TTGTCAGAAG 
TTGAAGAGAA TGCCAATTTG TCTTCCGTTG CTTGAAAATG GCCAGCCATC TGCTCTCCCT 
TCTCTTCAAA ATATCTTTAT ATATCCAAGA GAATGGTGGG AGTCTGTAGT GGAGTGGGAT 
CATCCTAATG CTAAGAACGT CCTTCGTCCC TTCGTAAAGT TTTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC18107958 cDNA ORF clone, Populus trichocarpa(black cottonwood)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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