SPEF2 gene cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

mRNA	Protein	Name
XM_016431614.1	XP_016287100.1	sperm flagellar protein 2 isoform X10
XM_016431611.1	XP_016287097.1	sperm flagellar protein 2 isoform X6
XM_016431613.1	XP_016287099.1	sperm flagellar protein 2 isoform X8
XM_016431606.1	XP_016287092.1	sperm flagellar protein 2 isoform X1
XM_016431612.1	XP_016287098.1	sperm flagellar protein 2 isoform X7
XM_016431608.1	XP_016287094.1	sperm flagellar protein 2 isoform X3
XM_007487458.2	XP_007487520.2	sperm flagellar protein 2 isoform X9
XM_016431607.1	XP_016287093.1	sperm flagellar protein 2 isoform X2
XM_016431609.1	XP_016287095.1	sperm flagellar protein 2 isoform X4
XM_016431610.1	XP_016287096.1	sperm flagellar protein 2 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMc41050	XM_016431614.1 Latest version!	Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMc41045	XM_016431610.1 Latest version!	Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMc41046	XM_016431611.1 Latest version!	Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMc41047	XM_016431612.1 Latest version!	Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMc41048	XM_016431613.1 Latest version!	Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMc41049	XM_007487458.2 Latest version!	Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMc41041	XM_016431606.1 Latest version!	Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMc41042	XM_016431607.1 Latest version!	Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMc41043	XM_016431608.1 Latest version!	Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMc41044	XM_016431609.1 Latest version!	Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

SPEF2 ( XM_016431614.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016287100.1 Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMc41050
Clone ID Related Accession (Same CDS sequence)	XM_016431614.1
Accession Version	XM_016431614.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	sperm flagellar protein 2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008803.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCATAA ATCAAGCAAA ACTTCTTGAG GAAGCCCTTA CAGGGTTCAA CACAGATCAA 
CCAGAACATG AAATAAAGGC TCACAAAAAA TCAACATTGG TTTTTGATCC ATCTGCTCCC 
AAAGACCCCC CTCCTCCCCC ACCTGCATTT GATTTTGTTA TGTTGCTGGA TATAACTGAC 
ACATTGGTGC TGAATCGTGT GATGGGAGAG GAGAAAAGAA ACACTGACAC AACTGGTATA 
CCAGAAAGTG CAACTTCAGA AATGTTTCCT GAAGAACAAG AAAAGTTTGA ACTTTTGAGG 
GATCAGATAC AACACAGAAT TTTAGGTTTC TTGGACAACT GGCCAGAGCT GGAAAAATGG 
TTTTCAGGAA AACAAGACAT TCTAATCACA GTCAATGCAG AAGTAGATAA AAGGGCTTTA 
GCCACAAAAC TACAAGAGCT TTTTATTGCT GAACTGAGGA AAAAAGAAGC TAAAGCAAAA 
CAGGAATTAG AAGAACAGGA AGAGGAAAGG AAAAAGAAAG AAGCCCTCCC ACCTGAACCG 
CCACCTCCAC CTCCCCCTGA ACCAGAAGAA GAGCCAAAGG AAGCAAAAGG GAAAGGAAAA 
GGGAAAGGTA AAGGTGCTAA AGAAGGAAAA GGAAAGAAAG ACACTTCTCC CAAGGGCAAA 
GATGCTGCTA AAGAAGCAAA AGCAAAGAAA AGTGAAACTT CTCCAAAAGG CAAAGGAAGG 
TCACCAGACA AGAGGTCACC TGAAGATAAA ACATCACCTC CTGCACCTCC AGGACCACCA 
CCAGTCAAAG CAGGATCAGA AGACTGGGTC TATGTGGATG AGCCACTTCC TCAGGAAATA 
TTTGAATATC TAGACACTTT TTGGGAAGTA ATAGAAAGTT CATACATAAA TACCAGCAAA 
GCTATATTCA GACAGATAAG AGATGAGAGA CATAGTGTAC TTTATTATCT ACATAATGTC 
AGACTAAATT ACCAGGGATA TCTGAAACGG CCAGATCACA AGCAGGAATT TGTATCACAA 
TGGCAGGAGG ATTTTAACAA CCTCCCTGAA GACTTATGGG AGGATGAGGA AACAAAAGCT 
GAACTACACC AAAGAGTCAA TGATTTAAGG GACCGCCTGT GGGATATATG TGATACTCGA 
AAGGAAGAGG CAGAACAAGA ACGTGCTGAT ATCATTGATG AATCCTGGTT ACAAGATCAT 
CTTGGAATTC TTATGAATCA TTTCTTTTCT TTGATGCAGG TGGAACTTAG CCGTTTCCAG 
GACATAAAGA CTTTTCTACA AGATTACTAT AGGGGAATGG AAGGAAAAAT CCCCACCGAC 
CTTGCTAAAT CTGGATATAC TCGAGTTCCC CTTATAGATC TTGATGAAGA GGATACCGAT 
ATCACCTCCA AAATCCCTGT AATTCCTCGG AGACAGCCTT CTCCTGAAGG ACTATTAAAC 
AAGCCCAGAA GTAAACTATC CCTGAGAGGA GGCAGACCTG ATTACTGCTT AGAAAATGTC 
CAACTCAAAT TTGAAAGAGA TGAGAAGACC CTTATGACTA TATGGCAGAA TACATCTTCA 
GCCATCAACC TCTTGGTAAA TTCTGAAATA CAGTTGAGGA AACTAGAAGA TGATAAAGAA 
AACAAACCAA CATCAACTAA AGAAAAATCT CCTCAAGATG GTGGCAAAGG TCCTAAAGGT 
GCTAAGAAAA AGCCAGATAA GAAGAAAGTT CCATCACCAG TTGAAGCCGT TTCTATGGTT 
CTAACACCAG AAGAACAGGA GGCTCTTGAT AAGAGGAATG AATTGAAAAT GAAAATAAAG 
GAAGAACATA TTACAGCTCT TCAGTGTGAA GCACAAGCTT CAATATTCCG ACTGGAACTG 
ATCAAGATGA AGGCCTTATC TTACATGGAA GAGCTGCTAG GAAGAGCAGT TGAGGTTTAC 
AAACTGATGG ACAAATGGCT CGGAGCCCGA TACTTAGCTG AAATGGCAAG TGTGGAAAAA 
TTAACTGAAG TGGCTCACTA CCACATTGAA ACCTCAACAA GGATTCAGAA TGAACTTATT 
TTAAATGAAG AAGATTTCTA CATCAATGGT GATGTGAAAG TCTTTCCAGA TCCGCTTCCT 
CCCGTCCGGC CTCCACCTGT GGAGAAAGAA ATGAATACTT TTCTCACTCT TGAACAACTC 
AACACCCTTC GAAATGAGTT TCTCCAGATG GCACCAAAAG CCAAAACACC ATGGAAGTAT 
TTTAAAAAAG GAATCTCTTC AGTTGATACT AGAAGACATG CTGAGGAAAA GAAAAGGCTA 
AGAAATCTTG ACTATTACCT ACCTAAGAGG AACAACCAAT TTTTGAAAAT TTGGCTAAAA 
AAATACCCTT GGCTCGTGTA TGATGACAGG TCAGACTTAA TGTTCTGTGG CTTGTGTCGC 
AAACATGGAG TGAGCTCAGG AGGAAACCAT GTTAGCTTTT TTTATGGCAC CAACAACTTT 
AGGACTGAAT TTCTCCAAGC ACATGACAAT AGTGAGGCAC ACACGAAGGC TGTTCTAGCA 
GAAGCAACAG GGGTTTCTTC CCTGACTGAT GCTGCCAAGG AGCAGATTCT AAAGAACAGG 
AACAAAGTAA TCTTGGGAAG AATAGAAAAC CTCTTTAGAA CATGTCATGC TATTGCCAAG 
ACTGGACGTC CCCTCAAAGA CTTTGTCTGG ATGTGTAAAC TGGATGATAT GAAGGGTGTA 
GACGTTGGCC CAATATTCAG GACTGACAAG TCAGCCAGAA CTTTCATATA CTTTATTGCT 
GAAGTAGAAC GCATATGTCT TCGAGAGAAA TTAGAAAAAT GTAAGTTCTT CTCACTCATC 
AGTGATGGTG GCTCAGGCAG CTTCATAGAA GACACCAAAG TTGTTTACAT CCAGTTTGCA 
TATGAGGGCA GGGTCTGCTG TCAGGTTGTT GGAGTACAGA CTGTGGACAA AATTGAACCG 
GTGGCAATAA AAAGTGCCAT TGAAAAAACA CTTGAGATAA ATCTTCAACT AAATCTGTCT 
AGCAAAGACT GGTCCAAAAA ACTCGTTGGG TTTGGAAGTG ATGGTGCATC TGTCATGGTG 
GGAGAGAACA ATGGTGTAGC CAGGCTTCTG AAAGAAATTC AGCCATGTGT GCAAACAGTC 
CACTGCTTTG CACATAGACT TGAACTAGCC TACCAAGAAG CATTGCAGAG CATCCAATTA 
TACAGTACTA TGAATGATCT TCTCCAGAAT ATATATTATT TTTATCATGA TTCACCTTCA 
AATACAAAAG CTCTGATAGC TGCCTTTGAA GACCTTAAAA TCCGCCCTGT GATTCCCTCT 
CGAGTTGGCA ACAACAGATG GCTCCCTCGG TTACAGATGG CTCTACAAAT CTTTCTCAAA 
GGATATTCTC CAATTGTCAA GCAATTAAGA ACAATGCATG AAGGCAGAAG TTCCGCTGAT 
CAACAGAAAG CTCAAGAGCT CCTACAGTTC CTTCTCCAAG CTGAGGTTGT CAGATTTGCT 
CACTTCTTGC TGGATGTAAT CAACGTCCTC AGCATTTTGT CTCGAGTCAA TCAGGACAGA 
AATTCATCAA TAGGAGATGT ATTTGCAACC CTACAATCAA CACTAGGAAC ACTCAAGATG 
TATAAAAAAA GACCAGGTCC AAAGGAACGT CTGACAGAGT CAGCCACACA CTTTCATGGT 
AACCAACTGA CAGGAAATGG AAATATCTCT GAAGTACGAA ACACAGTCTT AACAAATCTC 
TCAAAGCATC TTCATGATTG TTTCAGTGAT GCCAGCCATG ATGTGGTGAG GGCGACCACT 
ATTGGAAGTT TCAAATTATG GCCAAGAAAG CTAGATCAAG AGTTTGGTGA GAAAGAGGTA 
ACAATTCTTA CAAAGCATTA TGAACCTGTG TTGGAAGGGG CCAATGTGAA CATTGATGAA 
GTGGACACAG AATGGAGCAT GATGAAATTA GAGATTTATA ATCGATTTCA GAACATCCGA 
AAGTTAACCT GGGATATTGT GAATTCCATC TACTCATACA AGTATCCCAA TATATTGACC 
CTAGTGGATC TGGTGCTGAC ACTCCCTGCA AGTTCAGCTG AGGCGGAACG TGGGTTCAGT 
CAGATGAAAC TAGCCAAGTC ACAAATGCAT TTTAAAACAG ATCCTGAGAA CATGACAGAC 
ATTTTAATAA TCCAGTTGAA TTCTCCAGAC ATCAATAAGT TTGATCCCAA GAAAGCCATC 
CATCTATGGA ATACTTCTTG GCAGAGAAAT ACACTTCCAC CAATTGACCA CACAATAACC 
AAGTCAGATT GCTCCTCTAG CTCAGATAGT CTTTATGAAA GTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016287100.1
CDS	124..4431
Translation	MTINQAKLLEEALTGFNTDQPEHEIKAHKKSTLVFDPSAPKDPPPPPPAFDFVMLLDITDTLVLNRVMGEEKRNTDTTGIPESATSEMFPEEQEKFELLRDQIQHRILGFLDNWPELEKWFSGKQDILITVNAEVDKRALATKLQELFIAELRKKEAKAKQELEEQEEERKKKEALPPEPPPPPPPEPEEEPKEAKGKGKGKGKGAKEGKGKKDTSPKGKDAAKEAKAKKSETSPKGKGRSPDKRSPEDKTSPPAPPGPPPVKAGSEDWVYVDEPLPQEIFEYLDTFWEVIESSYINTSKAIFRQIRDERHSVLYYLHNVRLNYQGYLKRPDHKQEFVSQWQEDFNNLPEDLWEDEETKAELHQRVNDLRDRLWDICDTRKEEAEQERADIIDESWLQDHLGILMNHFFSLMQVELSRFQDIKTFLQDYYRGMEGKIPTDLAKSGYTRVPLIDLDEEDTDITSKIPVIPRRQPSPEGLLNKPRSKLSLRGGRPDYCLENVQLKFERDEKTLMTIWQNTSSAINLLVNSEIQLRKLEDDKENKPTSTKEKSPQDGGKGPKGAKKKPDKKKVPSPVEAVSMVLTPEEQEALDKRNELKMKIKEEHITALQCEAQASIFRLELIKMKALSYMEELLGRAVEVYKLMDKWLGARYLAEMASVEKLTEVAHYHIETSTRIQNELILNEEDFYINGDVKVFPDPLPPVRPPPVEKEMNTFLTLEQLNTLRNEFLQMAPKAKTPWKYFKKGISSVDTRRHAEEKKRLRNLDYYLPKRNNQFLKIWLKKYPWLVYDDRSDLMFCGLCRKHGVSSGGNHVSFFYGTNNFRTEFLQAHDNSEAHTKAVLAEATGVSSLTDAAKEQILKNRNKVILGRIENLFRTCHAIAKTGRPLKDFVWMCKLDDMKGVDVGPIFRTDKSARTFIYFIAEVERICLREKLEKCKFFSLISDGGSGSFIEDTKVVYIQFAYEGRVCCQVVGVQTVDKIEPVAIKSAIEKTLEINLQLNLSSKDWSKKLVGFGSDGASVMVGENNGVARLLKEIQPCVQTVHCFAHRLELAYQEALQSIQLYSTMNDLLQNIYYFYHDSPSNTKALIAAFEDLKIRPVIPSRVGNNRWLPRLQMALQIFLKGYSPIVKQLRTMHEGRSSADQQKAQELLQFLLQAEVVRFAHFLLDVINVLSILSRVNQDRNSSIGDVFATLQSTLGTLKMYKKRPGPKERLTESATHFHGNQLTGNGNISEVRNTVLTNLSKHLHDCFSDASHDVVRATTIGSFKLWPRKLDQEFGEKEVTILTKHYEPVLEGANVNIDEVDTEWSMMKLEIYNRFQNIRKLTWDIVNSIYSYKYPNILTLVDLVLTLPASSAEAERGFSQMKLAKSQMHFKTDPENMTDILIIQLNSPDINKFDPKKAIHLWNTSWQRNTLPPIDHTITKSDCSSSSDSLYESD

Target ORF information:

RefSeq Version	XM_016431614.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016431614.1

ORF Insert Sequence:

ATGACCATAA ATCAAGCAAA ACTTCTTGAG GAAGCCCTTA CAGGGTTCAA CACAGATCAA 
CCAGAACATG AAATAAAGGC TCACAAAAAA TCAACATTGG TTTTTGATCC ATCTGCTCCC 
AAAGACCCCC CTCCTCCCCC ACCTGCATTT GATTTTGTTA TGTTGCTGGA TATAACTGAC 
ACATTGGTGC TGAATCGTGT GATGGGAGAG GAGAAAAGAA ACACTGACAC AACTGGTATA 
CCAGAAAGTG CAACTTCAGA AATGTTTCCT GAAGAACAAG AAAAGTTTGA ACTTTTGAGG 
GATCAGATAC AACACAGAAT TTTAGGTTTC TTGGACAACT GGCCAGAGCT GGAAAAATGG 
TTTTCAGGAA AACAAGACAT TCTAATCACA GTCAATGCAG AAGTAGATAA AAGGGCTTTA 
GCCACAAAAC TACAAGAGCT TTTTATTGCT GAACTGAGGA AAAAAGAAGC TAAAGCAAAA 
CAGGAATTAG AAGAACAGGA AGAGGAAAGG AAAAAGAAAG AAGCCCTCCC ACCTGAACCG 
CCACCTCCAC CTCCCCCTGA ACCAGAAGAA GAGCCAAAGG AAGCAAAAGG GAAAGGAAAA 
GGGAAAGGTA AAGGTGCTAA AGAAGGAAAA GGAAAGAAAG ACACTTCTCC CAAGGGCAAA 
GATGCTGCTA AAGAAGCAAA AGCAAAGAAA AGTGAAACTT CTCCAAAAGG CAAAGGAAGG 
TCACCAGACA AGAGGTCACC TGAAGATAAA ACATCACCTC CTGCACCTCC AGGACCACCA 
CCAGTCAAAG CAGGATCAGA AGACTGGGTC TATGTGGATG AGCCACTTCC TCAGGAAATA 
TTTGAATATC TAGACACTTT TTGGGAAGTA ATAGAAAGTT CATACATAAA TACCAGCAAA 
GCTATATTCA GACAGATAAG AGATGAGAGA CATAGTGTAC TTTATTATCT ACATAATGTC 
AGACTAAATT ACCAGGGATA TCTGAAACGG CCAGATCACA AGCAGGAATT TGTATCACAA 
TGGCAGGAGG ATTTTAACAA CCTCCCTGAA GACTTATGGG AGGATGAGGA AACAAAAGCT 
GAACTACACC AAAGAGTCAA TGATTTAAGG GACCGCCTGT GGGATATATG TGATACTCGA 
AAGGAAGAGG CAGAACAAGA ACGTGCTGAT ATCATTGATG AATCCTGGTT ACAAGATCAT 
CTTGGAATTC TTATGAATCA TTTCTTTTCT TTGATGCAGG TGGAACTTAG CCGTTTCCAG 
GACATAAAGA CTTTTCTACA AGATTACTAT AGGGGAATGG AAGGAAAAAT CCCCACCGAC 
CTTGCTAAAT CTGGATATAC TCGAGTTCCC CTTATAGATC TTGATGAAGA GGATACCGAT 
ATCACCTCCA AAATCCCTGT AATTCCTCGG AGACAGCCTT CTCCTGAAGG ACTATTAAAC 
AAGCCCAGAA GTAAACTATC CCTGAGAGGA GGCAGACCTG ATTACTGCTT AGAAAATGTC 
CAACTCAAAT TTGAAAGAGA TGAGAAGACC CTTATGACTA TATGGCAGAA TACATCTTCA 
GCCATCAACC TCTTGGTAAA TTCTGAAATA CAGTTGAGGA AACTAGAAGA TGATAAAGAA 
AACAAACCAA CATCAACTAA AGAAAAATCT CCTCAAGATG GTGGCAAAGG TCCTAAAGGT 
GCTAAGAAAA AGCCAGATAA GAAGAAAGTT CCATCACCAG TTGAAGCCGT TTCTATGGTT 
CTAACACCAG AAGAACAGGA GGCTCTTGAT AAGAGGAATG AATTGAAAAT GAAAATAAAG 
GAAGAACATA TTACAGCTCT TCAGTGTGAA GCACAAGCTT CAATATTCCG ACTGGAACTG 
ATCAAGATGA AGGCCTTATC TTACATGGAA GAGCTGCTAG GAAGAGCAGT TGAGGTTTAC 
AAACTGATGG ACAAATGGCT CGGAGCCCGA TACTTAGCTG AAATGGCAAG TGTGGAAAAA 
TTAACTGAAG TGGCTCACTA CCACATTGAA ACCTCAACAA GGATTCAGAA TGAACTTATT 
TTAAATGAAG AAGATTTCTA CATCAATGGT GATGTGAAAG TCTTTCCAGA TCCGCTTCCT 
CCCGTCCGGC CTCCACCTGT GGAGAAAGAA ATGAATACTT TTCTCACTCT TGAACAACTC 
AACACCCTTC GAAATGAGTT TCTCCAGATG GCACCAAAAG CCAAAACACC ATGGAAGTAT 
TTTAAAAAAG GAATCTCTTC AGTTGATACT AGAAGACATG CTGAGGAAAA GAAAAGGCTA 
AGAAATCTTG ACTATTACCT ACCTAAGAGG AACAACCAAT TTTTGAAAAT TTGGCTAAAA 
AAATACCCTT GGCTCGTGTA TGATGACAGG TCAGACTTAA TGTTCTGTGG CTTGTGTCGC 
AAACATGGAG TGAGCTCAGG AGGAAACCAT GTTAGCTTTT TTTATGGCAC CAACAACTTT 
AGGACTGAAT TTCTCCAAGC ACATGACAAT AGTGAGGCAC ACACGAAGGC TGTTCTAGCA 
GAAGCAACAG GGGTTTCTTC CCTGACTGAT GCTGCCAAGG AGCAGATTCT AAAGAACAGG 
AACAAAGTAA TCTTGGGAAG AATAGAAAAC CTCTTTAGAA CATGTCATGC TATTGCCAAG 
ACTGGACGTC CCCTCAAAGA CTTTGTCTGG ATGTGTAAAC TGGATGATAT GAAGGGTGTA 
GACGTTGGCC CAATATTCAG GACTGACAAG TCAGCCAGAA CTTTCATATA CTTTATTGCT 
GAAGTAGAAC GCATATGTCT TCGAGAGAAA TTAGAAAAAT GTAAGTTCTT CTCACTCATC 
AGTGATGGTG GCTCAGGCAG CTTCATAGAA GACACCAAAG TTGTTTACAT CCAGTTTGCA 
TATGAGGGCA GGGTCTGCTG TCAGGTTGTT GGAGTACAGA CTGTGGACAA AATTGAACCG 
GTGGCAATAA AAAGTGCCAT TGAAAAAACA CTTGAGATAA ATCTTCAACT AAATCTGTCT 
AGCAAAGACT GGTCCAAAAA ACTCGTTGGG TTTGGAAGTG ATGGTGCATC TGTCATGGTG 
GGAGAGAACA ATGGTGTAGC CAGGCTTCTG AAAGAAATTC AGCCATGTGT GCAAACAGTC 
CACTGCTTTG CACATAGACT TGAACTAGCC TACCAAGAAG CATTGCAGAG CATCCAATTA 
TACAGTACTA TGAATGATCT TCTCCAGAAT ATATATTATT TTTATCATGA TTCACCTTCA 
AATACAAAAG CTCTGATAGC TGCCTTTGAA GACCTTAAAA TCCGCCCTGT GATTCCCTCT 
CGAGTTGGCA ACAACAGATG GCTCCCTCGG TTACAGATGG CTCTACAAAT CTTTCTCAAA 
GGATATTCTC CAATTGTCAA GCAATTAAGA ACAATGCATG AAGGCAGAAG TTCCGCTGAT 
CAACAGAAAG CTCAAGAGCT CCTACAGTTC CTTCTCCAAG CTGAGGTTGT CAGATTTGCT 
CACTTCTTGC TGGATGTAAT CAACGTCCTC AGCATTTTGT CTCGAGTCAA TCAGGACAGA 
AATTCATCAA TAGGAGATGT ATTTGCAACC CTACAATCAA CACTAGGAAC ACTCAAGATG 
TATAAAAAAA GACCAGGTCC AAAGGAACGT CTGACAGAGT CAGCCACACA CTTTCATGGT 
AACCAACTGA CAGGAAATGG AAATATCTCT GAAGTACGAA ACACAGTCTT AACAAATCTC 
TCAAAGCATC TTCATGATTG TTTCAGTGAT GCCAGCCATG ATGTGGTGAG GGCGACCACT 
ATTGGAAGTT TCAAATTATG GCCAAGAAAG CTAGATCAAG AGTTTGGTGA GAAAGAGGTA 
ACAATTCTTA CAAAGCATTA TGAACCTGTG TTGGAAGGGG CCAATGTGAA CATTGATGAA 
GTGGACACAG AATGGAGCAT GATGAAATTA GAGATTTATA ATCGATTTCA GAACATCCGA 
AAGTTAACCT GGGATATTGT GAATTCCATC TACTCATACA AGTATCCCAA TATATTGACC 
CTAGTGGATC TGGTGCTGAC ACTCCCTGCA AGTTCAGCTG AGGCGGAACG TGGGTTCAGT 
CAGATGAAAC TAGCCAAGTC ACAAATGCAT TTTAAAACAG ATCCTGAGAA CATGACAGAC 
ATTTTAATAA TCCAGTTGAA TTCTCCAGAC ATCAATAAGT TTGATCCCAA GAAAGCCATC 
CATCTATGGA ATACTTCTTG GCAGAGAAAT ACACTTCCAC CAATTGACCA CACAATAACC 
AAGTCAGATT GCTCCTCTAG CTCAGATAGT CTTTATGAAA GTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_016431610.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016287096.1 Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMc41045
Clone ID Related Accession (Same CDS sequence)	XM_016431610.1
Accession Version	XM_016431610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	sperm flagellar protein 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008803.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAAT ATTTAGTTAA GAGTGGATCA AAAGTTCCTT CGGCAGCCCA GACAACTGCT 
GACTTGTTAA GCACGTATTC AGATGAGGAA TACATTAAAA AAATTCAAAA ACGTCTAGAG 
GAAGATACCT TAGCCCGGGA ACAAAGGGAG AAAAGACGCA GAAAATTGTT AATGGATCAA 
TTGATAGCCC ATGAAGCTCA AGAGGAGGCT TACCGAGAAG AACAACTCAT TAACCGACTA 
ATGAGACAAT CTCAGCAGGA ACGTCGGATT GCTGTGCAAC TCATGCATGT TCGACACGAA 
AAGGAAGTTT TATGGCAAAA CAGGGTTTTC AGAGAAAAAC AATATGAAGA AAGACGGCTG 
AAAGATTTCC AGGATGCTCT TGACAGAGAA GCAGCTCTTG CAAGACAAGC CAAGCTGGAG 
TATGAAGAAC AAGCCCAAAA GGAAATAGAA ATTCATGAAA GAATAGCAGC AGAAAGATCT 
CAAGCTCGTT ATAAAAAGCA TTATTCAATG TGCACAGAAA TCTTGGACAA TATCATTGAT 
TTATCAACTA AAATAGCAGA ATATCGAGAA TTAACACATA ATCTTATTCC AGTTAAAATT 
ATGCGTGATT GGAAGGAGCT GTTTTTCAGT GGAAAGCCAA TTTATGAAAT AGCCTCCATT 
GACTCCAAGC CATCTCGTTT TACCTCAGAA CAACTAATTG AGCTATCCAA AAGGAATTTA 
CTAGATGACA ATGATTATGA GGAGTACAAG AACTTGACAG GAGAGTGGGT CCTCCCAGAA 
GATATGGCTG ATGTTCCCCT ACCTTCCAAC AATAACATAT TGGGTCATGT GATTCGAGTA 
GTTCATGAAA AAGTTTACCC TCCAAAACCA GCATATATTG CAACTCAGTT ACCTTCATCT 
TCGATTAAAG GGTGCATGCT GGGCAAGACA TGCAGTGGGA AAACCAGCTG TCTAAAGATT 
TTAGAAGAAG TTTTCCATAT TGAGATACTT TGTATGGATA CTCTGGTTCA AGAGGCTATT 
CAGGCTTTCC TTAGAAATGA AACTGTAACT GAGGTGGAAA CACATAAAGA AGATTTATCA 
AAAGAAGTTC AAGATATTAA GGATTCAAAC ATGACGACTA TTGCAGAAAT AATTCCTACT 
GAGGAGAAAA AAGATGAGAA TCTACCACAA CAAGATGAAG TGATTATTAA ACCACCATCA 
GCCCAGGATG TGAGAATAAG ACTTACTAAT CGTGCTCAAC TTGGTTCAAA ATGTGAAAAG 
TTGCTCAAGA GAGGAAGAAG TATCCCCGAT GTACTGCTAA TTCAAATCAT GGTGAATGCT 
ATTAATGAAA TATCATCAGA CAAAGGATGG ATCTTGGATG GTTTTCCAAT GACCATAAAT 
CAAGCAAAAC TTCTTGAGGA AGCCCTTACA GGGTTCAACA CAGATCAACC AGAACATGAA 
ATAAAGGCTC ACAAAAAATC AACATTGGTT TTTGATCCAT CTGCTCCCAA AGACCCCCCT 
CCTCCCCCAC CTGCATTTGA TTTTGTTATG TTGCTGGATA TAACTGACAC ATTGGTGCTG 
AATCGTGTGA TGGGAGAGGA GAAAAGAAAC ACTGACACAA CTGGTATACC AGAAAGTGCA 
ACTTCAGAAA TGTTTCCTGA AGAACAAGAA AAGTTTGAAC TTTTGAGGGA TCAGATACAA 
CACAGAATTT TAGGTTTCTT GGACAACTGG CCAGAGCTGG AAAAATGGTT TTCAGGAAAA 
CAAGACATTC TAATCACAGT CAATGCAGAA GTAGATAAAA GGGCTTTAGC CACAAAACTA 
CAAGAGCTTT TTATTGCTGA ACTGAGGAAA AAAGAAGCTA AAGCAAAACA GGAATTAGAA 
GAACAGGAAG AGGAAAGGAA AAAGAAAGAA GCCCTCCCAC CTGAACCGCC ACCTCCACCT 
CCCCCTGAAC CAGAAGAAGA GCCAAAGGAA GCAAAAGGGA AAGGAAAAGG GAAAGGTAAA 
GGTGCTAAAG AAGGAAAAGG AAAGAAAGAC ACTTCTCCCA AGGGCAAAGA TGCTGCTAAA 
GAAGCAAAAG CAAAGAAAAG TGAAACTTCT CCAAAAGGCA AAGGAAGGTC ACCAGACAAG 
AGGTCACCTG AAGATAAAAC ATCACCTCCT GCACCTCCAG GACCACCACC AGTCAAAGCA 
GGATCAGAAG ACTGGGTCTA TGTGGATGAG CCACTTCCTC AGGAAATATT TGAATATCTA 
GACACTTTTT GGGAAGTAAT AGAAAGTTCA TACATAAATA CCAGCAAAGC TATATTCAGA 
CAGATAAGAG ATGAGAGACA TAGTGTACTT TATTATCTAC ATAATGTCAG ACTAAATTAC 
CAGGGATATC TGAAACGGCC AGATCACAAG CAGGAATTTG TATCACAATG GCAGGAGGAT 
TTTAACAACC TCCCTGAAGA CTTATGGGAG GATGAGGAAA CAAAAGCTGA ACTACACCAA 
AGAGTCAATG ATTTAAGGGA CCGCCTGTGG GATATATGTG ATACTCGAAA GGAAGAGGCA 
GAACAAGAAC GTGCTGATAT CATTGATGAA TCCTGGTTAC AAGATCATCT TGGAATTCTT 
ATGAATCATT TCTTTTCTTT GATGCAGGTG GAACTTAGCC GTTTCCAGGA CATAAAGACT 
TTTCTACAAG ATTACTATAG GGGAATGGAA GGAAAAATCC CCACCGACCT TGCTAAATCT 
GGATATACTC GAGTTCCCCT TATAGATCTT GATGAAGAGG ATACCGATAT CACCTCCAAA 
ATCCCTGTAA TTCCTCGGAG ACAGCCTTCT CCTGAAGGAC TATTAAACAA GCCCAGAAGT 
AAACTATCCC TGAGAGGAGG CAGACCTGAT TACTGCTTAG AAAATGTCCA ACTCAAATTT 
GAAAGAGATG AGAAGACCCT TATGACTATA TGGCAGAATA CATCTTCAGC CATCAACCTC 
TTGGTAAATT CTGAAATACA GTTGAGGAAA CTAGAAGATG ATAAAGAAAA CAAACCAACA 
TCAACTAAAG AAAAATCTCC TCAAGATGGT GGCAAAGGTC CTAAAGGTGC TAAGAAAAAG 
CCAGATAAGA AGAAAGTTCC ATCACCAGTT GAAGCCGTTT CTATGGTTCT AACACCAGAA 
GAACAGGAGG CTCTTGATAA GAGGAATGAA TTGAAAATGA AAATAAAGGA AGAACATATT 
ACAGCTCTTC AGTGTGAAGC ACAAGCTTCA ATATTCCGAC TGGAACTGAT CAAGATGAAG 
GCCTTATCTT ACATGGAAGA GCTGCTAGGA AGAGCAGTTG AGGTTTACAA ACTGATGGAC 
AAATGGCTCG GAGCCCGATA CTTAGCTGAA ATGGCAAGTG TGGAAAAATT AACTGAAGTG 
GCTCACTACC ACATTGAAAC CTCAACAAGG ATTCAGAATG AACTTATTTT AAATGAAGAA 
GATTTCTACA TCAATGGTGA TGTGAAAGTC TTTCCAGATC CGCTTCCTCC CGTCCGGCCT 
CCACCTGTGG AGAAAGAAAT GAATACTTTT CTCACTCTTG AACAACTCAA CACCCTTCGA 
AATGAGTTTC TCCAGATGGC ACCAAAAGCC AAAACACCAT GGAAGTATTT TAAAAAAGGA 
ATCTCTTCAG TTGATACTAG AAGACATGCT GAGGAAAAGA AAAGGCTAAG AAATCTTGAC 
TATTACCTAC CTAAGAGGAA CAACCAATTT TTGAAAATTT GGCTAAAAAA ATACCCTTGG 
CTCGTGTATG ATGACAGGTC AGACTTAATG TTCTGTGGCT TGTGTCGCAA ACATGGAGTG 
AGCTCAGGAG GAAACCATGT TAGCTTTTTT TATGGCACCA ACAACTTTAG GACTGAATTT 
CTCCAAGCAC ATGACAATAG TGAGGCACAC ACGAAGGCTG TTCTAGCAGA AGCAACAGGG 
GTTTCTTCCC TGACTGATGC TGCCAAGGAG CAGATTCTAA AGAACAGGAA CAAAGTAATC 
TTGGGAAGAA TAGAAAACCT CTTTAGAACA TGTCATGCTA TTGCCAAGAC TGGACGTCCC 
CTCAAAGACT TTGTCTGGAT GTGTAAACTG GATGATATGA AGGGTGTAGA CGTTGGCCCA 
ATATTCAGGA CTGACAAGTC AGCCAGAACT TTCATATACT TTATTGCTGA AGTAGAACGC 
ATATGTCTTC GAGAGAAATT AGAAAAATGT AAGTTCTTCT CACTCATCAG TGATGGTGGC 
TCAGGCAGCT TCATAGAAGA CACCAAAGTT GTTTACATCC AGTTTGCATA TGAGGGCAGG 
GTCTGCTGTC AGGTTGTTGG AGTACAGACT GTGGACAAAA TTGAACCGGT GGCAATAAAA 
AGTGCCATTG AAAAAACACT TGAGATAAAT CTTCAACTAA ATCTGTCTAG CAAAGACTGG 
TCCAAAAAAC TCGTTGGGTT TGGAAGTGAT GGTGCATCTG TCATGGTGGG AGAGAACAAT 
GGTGTAGCCA GGCTTCTGAA AGAAATTCAG CCATGTGTGC AAACAGTCCA CTGCTTTGCA 
CATAGACTTG AACTAGCCTA CCAAGAAGCA TTGCAGAGCA TCCAATTATA CAGTACTATG 
AATGATCTTC TCCAGAATAT ATATTATTTT TATCATGATT CACCTTCAAA TACAAAAGCT 
CTGATAGCTG CCTTTGAAGA CCTTAAAATC CGCCCTGTGA TTCCCTCTCG AGTTGGCAAC 
AACAGATGGC TCCCTCGGTT ACAGATGGCT CTACAAATCT TTCTCAAAGG ATATTCTCCA 
ATTGTCAAGC AATTAAGAAC AATGCATGAA GGCAGAAGTT CCGCTGATCA ACAGAAAGCT 
CAAGAGCTCC TACAGTTCCT TCTCCAAGCT GAGGTTGTCA GATTTGCTCA CTTCTTGCTG 
GATGTAATCA ACGTCCTCAG CATTTTGTCT CGAGTCAATC AGGACAGAAA TTCATCAATA 
GGAGATGTAT TTGCAACCCT ACAATCAACA CTAGGAACAC TCAAGATGTA TAAAAAAAGA 
CCAGGTCCAA AGGAACGTCT GACAGAGTCA GCCACACACT TTCATGGTAA CCAACTGACA 
GGAAATGGAA ATATCTCTGA AGTACGAAAC ACAGTCTTAA CAAATCTCTC AAAGCATCTT 
CATGATTGTT TCAGTGATGC CAGCCATGAT GTGGTGAGGG CGACCACTAT TGGAAGTTTC 
AAATTATGGC CAAGAAAGCT AGATCAAGAG TTTGGTGAGA AAGAGGTAAC AATTCTTACA 
AAGCATTATG AACCTGTGTT GGAAGGGGCC AATGTGAACA TTGATGAAGT GGACACAGAA 
TGGAGCATGA TGAAATTAGA GATTTATAAT CGATTTCAGA ACATCCGAAA GTTAACCTGG 
GATATTGTGA ATTCCATCTA CTCATACAAG TATCCCAATA TATTGACCCT AGTGGATCTG 
GTGCTGACAC TCCCTGCAAG TTCAGCTGAG GCGGAACGTG GGTTCAGTCA GATGAAACTA 
GCCAAGTCAC AAATGCATTT TAAAACAGAT CCTGAGAACA TGACAGACAT TTTAATAATC 
CAGTTGAATT CTCCAGACAT CAATAAGTTT GATCCCAAGA AAGCCATCCA TCTATGGAAT 
ACTTCTTGGC AGAGAAATAC ACTTCCACCA ATTGACCACA CAATAACCAA GTCAGATTGC 
TCCTCTAGCT CAGATAGTCT TTATGAAAGT GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016287096.1
CDS	2398..8073
Translation	MNKYLVKSGSKVPSAAQTTADLLSTYSDEEYIKKIQKRLEEDTLAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRVFREKQYEERRLKDFQDALDREAALARQAKLEYEEQAQKEIEIHERIAAERSQARYKKHYSMCTEILDNIIDLSTKIAEYRELTHNLIPVKIMRDWKELFFSGKPIYEIASIDSKPSRFTSEQLIELSKRNLLDDNDYEEYKNLTGEWVLPEDMADVPLPSNNNILGHVIRVVHEKVYPPKPAYIATQLPSSSIKGCMLGKTCSGKTSCLKILEEVFHIEILCMDTLVQEAIQAFLRNETVTEVETHKEDLSKEVQDIKDSNMTTIAEIIPTEEKKDENLPQQDEVIIKPPSAQDVRIRLTNRAQLGSKCEKLLKRGRSIPDVLLIQIMVNAINEISSDKGWILDGFPMTINQAKLLEEALTGFNTDQPEHEIKAHKKSTLVFDPSAPKDPPPPPPAFDFVMLLDITDTLVLNRVMGEEKRNTDTTGIPESATSEMFPEEQEKFELLRDQIQHRILGFLDNWPELEKWFSGKQDILITVNAEVDKRALATKLQELFIAELRKKEAKAKQELEEQEEERKKKEALPPEPPPPPPPEPEEEPKEAKGKGKGKGKGAKEGKGKKDTSPKGKDAAKEAKAKKSETSPKGKGRSPDKRSPEDKTSPPAPPGPPPVKAGSEDWVYVDEPLPQEIFEYLDTFWEVIESSYINTSKAIFRQIRDERHSVLYYLHNVRLNYQGYLKRPDHKQEFVSQWQEDFNNLPEDLWEDEETKAELHQRVNDLRDRLWDICDTRKEEAEQERADIIDESWLQDHLGILMNHFFSLMQVELSRFQDIKTFLQDYYRGMEGKIPTDLAKSGYTRVPLIDLDEEDTDITSKIPVIPRRQPSPEGLLNKPRSKLSLRGGRPDYCLENVQLKFERDEKTLMTIWQNTSSAINLLVNSEIQLRKLEDDKENKPTSTKEKSPQDGGKGPKGAKKKPDKKKVPSPVEAVSMVLTPEEQEALDKRNELKMKIKEEHITALQCEAQASIFRLELIKMKALSYMEELLGRAVEVYKLMDKWLGARYLAEMASVEKLTEVAHYHIETSTRIQNELILNEEDFYINGDVKVFPDPLPPVRPPPVEKEMNTFLTLEQLNTLRNEFLQMAPKAKTPWKYFKKGISSVDTRRHAEEKKRLRNLDYYLPKRNNQFLKIWLKKYPWLVYDDRSDLMFCGLCRKHGVSSGGNHVSFFYGTNNFRTEFLQAHDNSEAHTKAVLAEATGVSSLTDAAKEQILKNRNKVILGRIENLFRTCHAIAKTGRPLKDFVWMCKLDDMKGVDVGPIFRTDKSARTFIYFIAEVERICLREKLEKCKFFSLISDGGSGSFIEDTKVVYIQFAYEGRVCCQVVGVQTVDKIEPVAIKSAIEKTLEINLQLNLSSKDWSKKLVGFGSDGASVMVGENNGVARLLKEIQPCVQTVHCFAHRLELAYQEALQSIQLYSTMNDLLQNIYYFYHDSPSNTKALIAAFEDLKIRPVIPSRVGNNRWLPRLQMALQIFLKGYSPIVKQLRTMHEGRSSADQQKAQELLQFLLQAEVVRFAHFLLDVINVLSILSRVNQDRNSSIGDVFATLQSTLGTLKMYKKRPGPKERLTESATHFHGNQLTGNGNISEVRNTVLTNLSKHLHDCFSDASHDVVRATTIGSFKLWPRKLDQEFGEKEVTILTKHYEPVLEGANVNIDEVDTEWSMMKLEIYNRFQNIRKLTWDIVNSIYSYKYPNILTLVDLVLTLPASSAEAERGFSQMKLAKSQMHFKTDPENMTDILIIQLNSPDINKFDPKKAIHLWNTSWQRNTLPPIDHTITKSDCSSSSDSLYESD

Target ORF information:

RefSeq Version	XM_016431610.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016431610.1

ORF Insert Sequence:

ATGAATAAAT ATTTAGTTAA GAGTGGATCA AAAGTTCCTT CGGCAGCCCA GACAACTGCT 
GACTTGTTAA GCACGTATTC AGATGAGGAA TACATTAAAA AAATTCAAAA ACGTCTAGAG 
GAAGATACCT TAGCCCGGGA ACAAAGGGAG AAAAGACGCA GAAAATTGTT AATGGATCAA 
TTGATAGCCC ATGAAGCTCA AGAGGAGGCT TACCGAGAAG AACAACTCAT TAACCGACTA 
ATGAGACAAT CTCAGCAGGA ACGTCGGATT GCTGTGCAAC TCATGCATGT TCGACACGAA 
AAGGAAGTTT TATGGCAAAA CAGGGTTTTC AGAGAAAAAC AATATGAAGA AAGACGGCTG 
AAAGATTTCC AGGATGCTCT TGACAGAGAA GCAGCTCTTG CAAGACAAGC CAAGCTGGAG 
TATGAAGAAC AAGCCCAAAA GGAAATAGAA ATTCATGAAA GAATAGCAGC AGAAAGATCT 
CAAGCTCGTT ATAAAAAGCA TTATTCAATG TGCACAGAAA TCTTGGACAA TATCATTGAT 
TTATCAACTA AAATAGCAGA ATATCGAGAA TTAACACATA ATCTTATTCC AGTTAAAATT 
ATGCGTGATT GGAAGGAGCT GTTTTTCAGT GGAAAGCCAA TTTATGAAAT AGCCTCCATT 
GACTCCAAGC CATCTCGTTT TACCTCAGAA CAACTAATTG AGCTATCCAA AAGGAATTTA 
CTAGATGACA ATGATTATGA GGAGTACAAG AACTTGACAG GAGAGTGGGT CCTCCCAGAA 
GATATGGCTG ATGTTCCCCT ACCTTCCAAC AATAACATAT TGGGTCATGT GATTCGAGTA 
GTTCATGAAA AAGTTTACCC TCCAAAACCA GCATATATTG CAACTCAGTT ACCTTCATCT 
TCGATTAAAG GGTGCATGCT GGGCAAGACA TGCAGTGGGA AAACCAGCTG TCTAAAGATT 
TTAGAAGAAG TTTTCCATAT TGAGATACTT TGTATGGATA CTCTGGTTCA AGAGGCTATT 
CAGGCTTTCC TTAGAAATGA AACTGTAACT GAGGTGGAAA CACATAAAGA AGATTTATCA 
AAAGAAGTTC AAGATATTAA GGATTCAAAC ATGACGACTA TTGCAGAAAT AATTCCTACT 
GAGGAGAAAA AAGATGAGAA TCTACCACAA CAAGATGAAG TGATTATTAA ACCACCATCA 
GCCCAGGATG TGAGAATAAG ACTTACTAAT CGTGCTCAAC TTGGTTCAAA ATGTGAAAAG 
TTGCTCAAGA GAGGAAGAAG TATCCCCGAT GTACTGCTAA TTCAAATCAT GGTGAATGCT 
ATTAATGAAA TATCATCAGA CAAAGGATGG ATCTTGGATG GTTTTCCAAT GACCATAAAT 
CAAGCAAAAC TTCTTGAGGA AGCCCTTACA GGGTTCAACA CAGATCAACC AGAACATGAA 
ATAAAGGCTC ACAAAAAATC AACATTGGTT TTTGATCCAT CTGCTCCCAA AGACCCCCCT 
CCTCCCCCAC CTGCATTTGA TTTTGTTATG TTGCTGGATA TAACTGACAC ATTGGTGCTG 
AATCGTGTGA TGGGAGAGGA GAAAAGAAAC ACTGACACAA CTGGTATACC AGAAAGTGCA 
ACTTCAGAAA TGTTTCCTGA AGAACAAGAA AAGTTTGAAC TTTTGAGGGA TCAGATACAA 
CACAGAATTT TAGGTTTCTT GGACAACTGG CCAGAGCTGG AAAAATGGTT TTCAGGAAAA 
CAAGACATTC TAATCACAGT CAATGCAGAA GTAGATAAAA GGGCTTTAGC CACAAAACTA 
CAAGAGCTTT TTATTGCTGA ACTGAGGAAA AAAGAAGCTA AAGCAAAACA GGAATTAGAA 
GAACAGGAAG AGGAAAGGAA AAAGAAAGAA GCCCTCCCAC CTGAACCGCC ACCTCCACCT 
CCCCCTGAAC CAGAAGAAGA GCCAAAGGAA GCAAAAGGGA AAGGAAAAGG GAAAGGTAAA 
GGTGCTAAAG AAGGAAAAGG AAAGAAAGAC ACTTCTCCCA AGGGCAAAGA TGCTGCTAAA 
GAAGCAAAAG CAAAGAAAAG TGAAACTTCT CCAAAAGGCA AAGGAAGGTC ACCAGACAAG 
AGGTCACCTG AAGATAAAAC ATCACCTCCT GCACCTCCAG GACCACCACC AGTCAAAGCA 
GGATCAGAAG ACTGGGTCTA TGTGGATGAG CCACTTCCTC AGGAAATATT TGAATATCTA 
GACACTTTTT GGGAAGTAAT AGAAAGTTCA TACATAAATA CCAGCAAAGC TATATTCAGA 
CAGATAAGAG ATGAGAGACA TAGTGTACTT TATTATCTAC ATAATGTCAG ACTAAATTAC 
CAGGGATATC TGAAACGGCC AGATCACAAG CAGGAATTTG TATCACAATG GCAGGAGGAT 
TTTAACAACC TCCCTGAAGA CTTATGGGAG GATGAGGAAA CAAAAGCTGA ACTACACCAA 
AGAGTCAATG ATTTAAGGGA CCGCCTGTGG GATATATGTG ATACTCGAAA GGAAGAGGCA 
GAACAAGAAC GTGCTGATAT CATTGATGAA TCCTGGTTAC AAGATCATCT TGGAATTCTT 
ATGAATCATT TCTTTTCTTT GATGCAGGTG GAACTTAGCC GTTTCCAGGA CATAAAGACT 
TTTCTACAAG ATTACTATAG GGGAATGGAA GGAAAAATCC CCACCGACCT TGCTAAATCT 
GGATATACTC GAGTTCCCCT TATAGATCTT GATGAAGAGG ATACCGATAT CACCTCCAAA 
ATCCCTGTAA TTCCTCGGAG ACAGCCTTCT CCTGAAGGAC TATTAAACAA GCCCAGAAGT 
AAACTATCCC TGAGAGGAGG CAGACCTGAT TACTGCTTAG AAAATGTCCA ACTCAAATTT 
GAAAGAGATG AGAAGACCCT TATGACTATA TGGCAGAATA CATCTTCAGC CATCAACCTC 
TTGGTAAATT CTGAAATACA GTTGAGGAAA CTAGAAGATG ATAAAGAAAA CAAACCAACA 
TCAACTAAAG AAAAATCTCC TCAAGATGGT GGCAAAGGTC CTAAAGGTGC TAAGAAAAAG 
CCAGATAAGA AGAAAGTTCC ATCACCAGTT GAAGCCGTTT CTATGGTTCT AACACCAGAA 
GAACAGGAGG CTCTTGATAA GAGGAATGAA TTGAAAATGA AAATAAAGGA AGAACATATT 
ACAGCTCTTC AGTGTGAAGC ACAAGCTTCA ATATTCCGAC TGGAACTGAT CAAGATGAAG 
GCCTTATCTT ACATGGAAGA GCTGCTAGGA AGAGCAGTTG AGGTTTACAA ACTGATGGAC 
AAATGGCTCG GAGCCCGATA CTTAGCTGAA ATGGCAAGTG TGGAAAAATT AACTGAAGTG 
GCTCACTACC ACATTGAAAC CTCAACAAGG ATTCAGAATG AACTTATTTT AAATGAAGAA 
GATTTCTACA TCAATGGTGA TGTGAAAGTC TTTCCAGATC CGCTTCCTCC CGTCCGGCCT 
CCACCTGTGG AGAAAGAAAT GAATACTTTT CTCACTCTTG AACAACTCAA CACCCTTCGA 
AATGAGTTTC TCCAGATGGC ACCAAAAGCC AAAACACCAT GGAAGTATTT TAAAAAAGGA 
ATCTCTTCAG TTGATACTAG AAGACATGCT GAGGAAAAGA AAAGGCTAAG AAATCTTGAC 
TATTACCTAC CTAAGAGGAA CAACCAATTT TTGAAAATTT GGCTAAAAAA ATACCCTTGG 
CTCGTGTATG ATGACAGGTC AGACTTAATG TTCTGTGGCT TGTGTCGCAA ACATGGAGTG 
AGCTCAGGAG GAAACCATGT TAGCTTTTTT TATGGCACCA ACAACTTTAG GACTGAATTT 
CTCCAAGCAC ATGACAATAG TGAGGCACAC ACGAAGGCTG TTCTAGCAGA AGCAACAGGG 
GTTTCTTCCC TGACTGATGC TGCCAAGGAG CAGATTCTAA AGAACAGGAA CAAAGTAATC 
TTGGGAAGAA TAGAAAACCT CTTTAGAACA TGTCATGCTA TTGCCAAGAC TGGACGTCCC 
CTCAAAGACT TTGTCTGGAT GTGTAAACTG GATGATATGA AGGGTGTAGA CGTTGGCCCA 
ATATTCAGGA CTGACAAGTC AGCCAGAACT TTCATATACT TTATTGCTGA AGTAGAACGC 
ATATGTCTTC GAGAGAAATT AGAAAAATGT AAGTTCTTCT CACTCATCAG TGATGGTGGC 
TCAGGCAGCT TCATAGAAGA CACCAAAGTT GTTTACATCC AGTTTGCATA TGAGGGCAGG 
GTCTGCTGTC AGGTTGTTGG AGTACAGACT GTGGACAAAA TTGAACCGGT GGCAATAAAA 
AGTGCCATTG AAAAAACACT TGAGATAAAT CTTCAACTAA ATCTGTCTAG CAAAGACTGG 
TCCAAAAAAC TCGTTGGGTT TGGAAGTGAT GGTGCATCTG TCATGGTGGG AGAGAACAAT 
GGTGTAGCCA GGCTTCTGAA AGAAATTCAG CCATGTGTGC AAACAGTCCA CTGCTTTGCA 
CATAGACTTG AACTAGCCTA CCAAGAAGCA TTGCAGAGCA TCCAATTATA CAGTACTATG 
AATGATCTTC TCCAGAATAT ATATTATTTT TATCATGATT CACCTTCAAA TACAAAAGCT 
CTGATAGCTG CCTTTGAAGA CCTTAAAATC CGCCCTGTGA TTCCCTCTCG AGTTGGCAAC 
AACAGATGGC TCCCTCGGTT ACAGATGGCT CTACAAATCT TTCTCAAAGG ATATTCTCCA 
ATTGTCAAGC AATTAAGAAC AATGCATGAA GGCAGAAGTT CCGCTGATCA ACAGAAAGCT 
CAAGAGCTCC TACAGTTCCT TCTCCAAGCT GAGGTTGTCA GATTTGCTCA CTTCTTGCTG 
GATGTAATCA ACGTCCTCAG CATTTTGTCT CGAGTCAATC AGGACAGAAA TTCATCAATA 
GGAGATGTAT TTGCAACCCT ACAATCAACA CTAGGAACAC TCAAGATGTA TAAAAAAAGA 
CCAGGTCCAA AGGAACGTCT GACAGAGTCA GCCACACACT TTCATGGTAA CCAACTGACA 
GGAAATGGAA ATATCTCTGA AGTACGAAAC ACAGTCTTAA CAAATCTCTC AAAGCATCTT 
CATGATTGTT TCAGTGATGC CAGCCATGAT GTGGTGAGGG CGACCACTAT TGGAAGTTTC 
AAATTATGGC CAAGAAAGCT AGATCAAGAG TTTGGTGAGA AAGAGGTAAC AATTCTTACA 
AAGCATTATG AACCTGTGTT GGAAGGGGCC AATGTGAACA TTGATGAAGT GGACACAGAA 
TGGAGCATGA TGAAATTAGA GATTTATAAT CGATTTCAGA ACATCCGAAA GTTAACCTGG 
GATATTGTGA ATTCCATCTA CTCATACAAG TATCCCAATA TATTGACCCT AGTGGATCTG 
GTGCTGACAC TCCCTGCAAG TTCAGCTGAG GCGGAACGTG GGTTCAGTCA GATGAAACTA 
GCCAAGTCAC AAATGCATTT TAAAACAGAT CCTGAGAACA TGACAGACAT TTTAATAATC 
CAGTTGAATT CTCCAGACAT CAATAAGTTT GATCCCAAGA AAGCCATCCA TCTATGGAAT 
ACTTCTTGGC AGAGAAATAC ACTTCCACCA ATTGACCACA CAATAACCAA GTCAGATTGC 
TCCTCTAGCT CAGATAGTCT TTATGAAAGT GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_016431611.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016287097.1 Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X6, mRNA.

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CloneID	OMc41046
Clone ID Related Accession (Same CDS sequence)	XM_016431611.1
Accession Version	XM_016431611.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	sperm flagellar protein 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008803.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGGTA GCATTTTACA CAGAACAGCA CAAAGACAAA GGGACCTTTT CATGGGAAAA 
GAGGCTCTTG CAAGACAAGC CAAGCTGGAG TATGAAGAAC AAGCCCAAAA GGAAATAGAA 
ATTCATGAAA GAATAGCAGC AGAAAGATCT CAAGCTCGTT ATAAAAAGCA TTATTCAATG 
TGCACAGAAA TCTTGGACAA TATCATTGAT TTATCAACTA AAATAGCAGA ATATCGAGAA 
TTAACACATA ATCTTATTCC AGTTAAAATT ATGCGTGATT GGAAGGAGCT GTTTTTCAGT 
GGAAAGCCAA TTTATGAAAT AGCCTCCATT GACTCCAAGC CATCTCGTTT TACCTCAGAA 
CAACTAATTG AGCTATCCAA AAGGAATTTA CTAGATGACA ATGATTATGA GGAGTACAAG 
AACTTGACAG GAGAGTGGGT CCTCCCAGAA GATATGGCTG ATGTTCCCCT ACCTTCCAAC 
AATAACATAT TGGGTCATGT GATTCGAGTA GTTCATGAAA AAGTTTACCC TCCAAAACCA 
GCATATATTG CAACTCAGTT ACCTTCATCT TCGATTAAAG GGTGCATGCT GGGCAAGACA 
TGCAGTGGGA AAACCAGCTG TCTAAAGATT TTAGAAGAAG TTTTCCATAT TGAGATACTT 
TGTATGGATA CTCTGGTTCA AGAGGCTATT CAGGCTTTCC TTAGAAATGA AACTGTAACT 
GAGGTGGAAA CACATAAAGA AGATTTATCA AAAGAAGTTC AAGATATTAA GGATTCAAAC 
ATGACGACTA TTGCAGAAAT AATTCCTACT GAGGAGAAAA AAGATGAGAA TCTACCACAA 
CAAGATGAAG TGATTATTAA ACCACCATCA GCCCAGGATG TGAGAATAAG ACTTACTAAT 
CGTGCTCAAC TTGGTTCAAA ATGTGAAAAG TTGCTCAAGA GAGGAAGAAG TATCCCCGAT 
GTACTGCTAA TTCAAATCAT GGTGAATGCT ATTAATGAAA TATCATCAGA CAAAGGATGG 
ATCTTGGATG GTTTTCCAAT GACCATAAAT CAAGCAAAAC TTCTTGAGGA AGCCCTTACA 
GGGTTCAACA CAGATCAACC AGAACATGAA ATAAAGGCTC ACAAAAAATC AACATTGGTT 
TTTGATCCAT CTGCTCCCAA AGACCCCCCT CCTCCCCCAC CTGCATTTGA TTTTGTTATG 
TTGCTGGATA TAACTGACAC ATTGGTGCTG AATCGTGTGA TGGGAGAGGA GAAAAGAAAC 
ACTGACACAA CTGGTATACC AGAAAGTGCA ACTTCAGAAA TGTTTCCTGA AGAACAAGAA 
AAGTTTGAAC TTTTGAGGGA TCAGATACAA CACAGAATTT TAGGTTTCTT GGACAACTGG 
CCAGAGCTGG AAAAATGGTT TTCAGGAAAA CAAGACATTC TAATCACAGT CAATGCAGAA 
GTAGATAAAA GGGCTTTAGC CACAAAACTA CAAGAGCTTT TTATTGCTGA ACTGAGGAAA 
AAAGAAGCTA AAGCAAAACA GGAATTAGAA GAACAGGAAG AGGAAAGGAA AAAGAAAGAA 
GCCCTCCCAC CTGAACCGCC ACCTCCACCT CCCCCTGAAC CAGAAGAAGA GCCAAAGGAA 
GCAAAAGGGA AAGGAAAAGG GAAAGGTAAA GGTGCTAAAG AAGGAAAAGG AAAGAAAGAC 
ACTTCTCCCA AGGGCAAAGA TGCTGCTAAA GAAGCAAAAG CAAAGAAAAG TGAAACTTCT 
CCAAAAGGCA AAGGAAGGTC ACCAGACAAG AGGTCACCTG AAGATAAAAC ATCACCTCCT 
GCACCTCCAG GACCACCACC AGTCAAAGCA GGATCAGAAG ACTGGGTCTA TGTGGATGAG 
CCACTTCCTC AGGAAATATT TGAATATCTA GACACTTTTT GGGAAGTAAT AGAAAGTTCA 
TACATAAATA CCAGCAAAGC TATATTCAGA CAGATAAGAG ATGAGAGACA TAGTGTACTT 
TATTATCTAC ATAATGTCAG ACTAAATTAC CAGGGATATC TGAAACGGCC AGATCACAAG 
CAGGAATTTG TATCACAATG GCAGGAGGAT TTTAACAACC TCCCTGAAGA CTTATGGGAG 
GATGAGGAAA CAAAAGCTGA ACTACACCAA AGAGTCAATG ATTTAAGGGA CCGCCTGTGG 
GATATATGTG ATACTCGAAA GGAAGAGGCA GAACAAGAAC GTGCTGATAT CATTGATGAA 
TCCTGGTTAC AAGATCATCT TGGAATTCTT ATGAATCATT TCTTTTCTTT GATGCAGGTG 
GAACTTAGCC GTTTCCAGGA CATAAAGACT TTTCTACAAG ATTACTATAG GGGAATGGAA 
GGAAAAATCC CCACCGACCT TGCTAAATCT GGATATACTC GAGTTCCCCT TATAGATCTT 
GATGAAGAGG ATACCGATAT CACCTCCAAA ATCCCTGTAA TTCCTCGGAG ACAGCCTTCT 
CCTGAAGGAC TATTAAACAA GCCCAGAAGT AAACTATCCC TGAGAGGAGG CAGACCTGAT 
TACTGCTTAG AAAATGTCCA ACTCAAATTT GAAAGAGATG AGAAGACCCT TATGACTATA 
TGGCAGAATA CATCTTCAGC CATCAACCTC TTGGTAAATT CTGAAATACA GTTGAGGAAA 
CTAGAAGATG ATAAAGAAAA CAAACCAACA TCAACTAAAG AAAAATCTCC TCAAGATGGT 
GGCAAAGGTC CTAAAGGTGC TAAGAAAAAG CCAGATAAGA AGAAAGTTCC ATCACCAGTT 
GAAGCCGTTT CTATGGTTCT AACACCAGAA GAACAGGAGG CTCTTGATAA GAGGAATGAA 
TTGAAAATGA AAATAAAGGA AGAACATATT ACAGCTCTTC AGTGTGAAGC ACAAGCTTCA 
ATATTCCGAC TGGAACTGAT CAAGATGAAG GCCTTATCTT ACATGGAAGA GCTGCTAGGA 
AGAGCAGTTG AGGTTTACAA ACTGATGGAC AAATGGCTCG GAGCCCGATA CTTAGCTGAA 
ATGGCAAGTG TGGAAAAATT AACTGAAGTG GCTCACTACC ACATTGAAAC CTCAACAAGG 
ATTCAGAATG AACTTATTTT AAATGAAGAA GATTTCTACA TCAATGGTGA TGTGAAAGTC 
TTTCCAGATC CGCTTCCTCC CGTCCGGCCT CCACCTGTGG AGAAAGAAAT GAATACTTTT 
CTCACTCTTG AACAACTCAA CACCCTTCGA AATGAGTTTC TCCAGATGGC ACCAAAAGCC 
AAAACACCAT GGAAGTATTT TAAAAAAGGA ATCTCTTCAG TTGATACTAG AAGACATGCT 
GAGGAAAAGA AAAGGCTAAG AAATCTTGAC TATTACCTAC CTAAGAGGAA CAACCAATTT 
TTGAAAATTT GGCTAAAAAA ATACCCTTGG CTCGTGTATG ATGACAGGTC AGACTTAATG 
TTCTGTGGCT TGTGTCGCAA ACATGGAGTG AGCTCAGGAG GAAACCATGT TAGCTTTTTT 
TATGGCACCA ACAACTTTAG GACTGAATTT CTCCAAGCAC ATGACAATAG TGAGGCACAC 
ACGAAGGCTG TTCTAGCAGA AGCAACAGGG GTTTCTTCCC TGACTGATGC TGCCAAGGAG 
CAGATTCTAA AGAACAGGAA CAAAGTAATC TTGGGAAGAA TAGAAAACCT CTTTAGAACA 
TGTCATGCTA TTGCCAAGAC TGGACGTCCC CTCAAAGACT TTGTCTGGAT GTGTAAACTG 
GATGATATGA AGGGTGTAGA CGTTGGCCCA ATATTCAGGA CTGACAAGTC AGCCAGAACT 
TTCATATACT TTATTGCTGA AGTAGAACGC ATATGTCTTC GAGAGAAATT AGAAAAATGT 
AAGTTCTTCT CACTCATCAG TGATGGTGGC TCAGGCAGCT TCATAGAAGA CACCAAAGTT 
GTTTACATCC AGTTTGCATA TGAGGGCAGG GTCTGCTGTC AGGTTGTTGG AGTACAGACT 
GTGGACAAAA TTGAACCGGT GGCAATAAAA AGTGCCATTG AAAAAACACT TGAGATAAAT 
CTTCAACTAA ATCTGTCTAG CAAAGACTGG TCCAAAAAAC TCGTTGGGTT TGGAAGTGAT 
GGTGCATCTG TCATGGTGGG AGAGAACAAT GGTGTAGCCA GGCTTCTGAA AGAAATTCAG 
CCATGTGTGC AAACAGTCCA CTGCTTTGCA CATAGACTTG AACTAGCCTA CCAAGAAGCA 
TTGCAGAGCA TCCAATTATA CAGTACTATG AATGATCTTC TCCAGAATAT ATATTATTTT 
TATCATGATT CACCTTCAAA TACAAAAGCT CTGATAGCTG CCTTTGAAGA CCTTAAAATC 
CGCCCTGTGA TTCCCTCTCG AGTTGGCAAC AACAGATGGC TCCCTCGGTT ACAGATGGCT 
CTACAAATCT TTCTCAAAGG ATATTCTCCA ATTGTCAAGC AATTAAGAAC AATGCATGAA 
GGCAGAAGTT CCGCTGATCA ACAGAAAGCT CAAGAGCTCC TACAGTTCCT TCTCCAAGCT 
GAGGTTGTCA GATTTGCTCA CTTCTTGCTG GATGTAATCA ACGTCCTCAG CATTTTGTCT 
CGAGTCAATC AGGACAGAAA TTCATCAATA GGAGATGTAT TTGCAACCCT ACAATCAACA 
CTAGGAACAC TCAAGATGTA TAAAAAAAGA CCAGGTCCAA AGGAACGTCT GACAGAGTCA 
GCCACACACT TTCATGGTAA CCAACTGACA GGAAATGGAA ATATCTCTGA AGTACGAAAC 
ACAGTCTTAA CAAATCTCTC AAAGCATCTT CATGATTGTT TCAGTGATGC CAGCCATGAT 
GTGGTGAGGG CGACCACTAT TGGAAGTTTC AAATTATGGC CAAGAAAGCT AGATCAAGAG 
TTTGGTGAGA AAGAGGTAAC AATTCTTACA AAGCATTATG AACCTGTGTT GGAAGGGGCC 
AATGTGAACA TTGATGAAGT GGACACAGAA TGGAGCATGA TGAAATTAGA GATTTATAAT 
CGATTTCAGA ACATCCGAAA GTTAACCTGG GATATTGTGA ATTCCATCTA CTCATACAAG 
TATCCCAATA TATTGACCCT AGTGGATCTG GTGCTGACAC TCCCTGCAAG TTCAGCTGAG 
GCGGAACGTG GGTTCAGTCA GATGAAACTA GCCAAGTCAC AAATGCATTT TAAAACAGAT 
CCTGAGAACA TGACAGACAT TTTAATAATC CAGTTGAATT CTCCAGACAT CAATAAGTTT 
GATCCCAAGA AAGCCATCCA TCTATGGAAT ACTTCTTGGC AGAGAAATAC ACTTCCACCA 
ATTGACCACA CAATAACCAA GTCAGATTGC TCCTCTAGCT CAGATAGTCT TTATGAAAGT 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016287097.1
CDS	790..6135
Translation	MQGSILHRTAQRQRDLFMGKEALARQAKLEYEEQAQKEIEIHERIAAERSQARYKKHYSMCTEILDNIIDLSTKIAEYRELTHNLIPVKIMRDWKELFFSGKPIYEIASIDSKPSRFTSEQLIELSKRNLLDDNDYEEYKNLTGEWVLPEDMADVPLPSNNNILGHVIRVVHEKVYPPKPAYIATQLPSSSIKGCMLGKTCSGKTSCLKILEEVFHIEILCMDTLVQEAIQAFLRNETVTEVETHKEDLSKEVQDIKDSNMTTIAEIIPTEEKKDENLPQQDEVIIKPPSAQDVRIRLTNRAQLGSKCEKLLKRGRSIPDVLLIQIMVNAINEISSDKGWILDGFPMTINQAKLLEEALTGFNTDQPEHEIKAHKKSTLVFDPSAPKDPPPPPPAFDFVMLLDITDTLVLNRVMGEEKRNTDTTGIPESATSEMFPEEQEKFELLRDQIQHRILGFLDNWPELEKWFSGKQDILITVNAEVDKRALATKLQELFIAELRKKEAKAKQELEEQEEERKKKEALPPEPPPPPPPEPEEEPKEAKGKGKGKGKGAKEGKGKKDTSPKGKDAAKEAKAKKSETSPKGKGRSPDKRSPEDKTSPPAPPGPPPVKAGSEDWVYVDEPLPQEIFEYLDTFWEVIESSYINTSKAIFRQIRDERHSVLYYLHNVRLNYQGYLKRPDHKQEFVSQWQEDFNNLPEDLWEDEETKAELHQRVNDLRDRLWDICDTRKEEAEQERADIIDESWLQDHLGILMNHFFSLMQVELSRFQDIKTFLQDYYRGMEGKIPTDLAKSGYTRVPLIDLDEEDTDITSKIPVIPRRQPSPEGLLNKPRSKLSLRGGRPDYCLENVQLKFERDEKTLMTIWQNTSSAINLLVNSEIQLRKLEDDKENKPTSTKEKSPQDGGKGPKGAKKKPDKKKVPSPVEAVSMVLTPEEQEALDKRNELKMKIKEEHITALQCEAQASIFRLELIKMKALSYMEELLGRAVEVYKLMDKWLGARYLAEMASVEKLTEVAHYHIETSTRIQNELILNEEDFYINGDVKVFPDPLPPVRPPPVEKEMNTFLTLEQLNTLRNEFLQMAPKAKTPWKYFKKGISSVDTRRHAEEKKRLRNLDYYLPKRNNQFLKIWLKKYPWLVYDDRSDLMFCGLCRKHGVSSGGNHVSFFYGTNNFRTEFLQAHDNSEAHTKAVLAEATGVSSLTDAAKEQILKNRNKVILGRIENLFRTCHAIAKTGRPLKDFVWMCKLDDMKGVDVGPIFRTDKSARTFIYFIAEVERICLREKLEKCKFFSLISDGGSGSFIEDTKVVYIQFAYEGRVCCQVVGVQTVDKIEPVAIKSAIEKTLEINLQLNLSSKDWSKKLVGFGSDGASVMVGENNGVARLLKEIQPCVQTVHCFAHRLELAYQEALQSIQLYSTMNDLLQNIYYFYHDSPSNTKALIAAFEDLKIRPVIPSRVGNNRWLPRLQMALQIFLKGYSPIVKQLRTMHEGRSSADQQKAQELLQFLLQAEVVRFAHFLLDVINVLSILSRVNQDRNSSIGDVFATLQSTLGTLKMYKKRPGPKERLTESATHFHGNQLTGNGNISEVRNTVLTNLSKHLHDCFSDASHDVVRATTIGSFKLWPRKLDQEFGEKEVTILTKHYEPVLEGANVNIDEVDTEWSMMKLEIYNRFQNIRKLTWDIVNSIYSYKYPNILTLVDLVLTLPASSAEAERGFSQMKLAKSQMHFKTDPENMTDILIIQLNSPDINKFDPKKAIHLWNTSWQRNTLPPIDHTITKSDCSSSSDSLYESD

Target ORF information:

RefSeq Version	XM_016431611.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016431611.1

ORF Insert Sequence:

ATGCAAGGTA GCATTTTACA CAGAACAGCA CAAAGACAAA GGGACCTTTT CATGGGAAAA 
GAGGCTCTTG CAAGACAAGC CAAGCTGGAG TATGAAGAAC AAGCCCAAAA GGAAATAGAA 
ATTCATGAAA GAATAGCAGC AGAAAGATCT CAAGCTCGTT ATAAAAAGCA TTATTCAATG 
TGCACAGAAA TCTTGGACAA TATCATTGAT TTATCAACTA AAATAGCAGA ATATCGAGAA 
TTAACACATA ATCTTATTCC AGTTAAAATT ATGCGTGATT GGAAGGAGCT GTTTTTCAGT 
GGAAAGCCAA TTTATGAAAT AGCCTCCATT GACTCCAAGC CATCTCGTTT TACCTCAGAA 
CAACTAATTG AGCTATCCAA AAGGAATTTA CTAGATGACA ATGATTATGA GGAGTACAAG 
AACTTGACAG GAGAGTGGGT CCTCCCAGAA GATATGGCTG ATGTTCCCCT ACCTTCCAAC 
AATAACATAT TGGGTCATGT GATTCGAGTA GTTCATGAAA AAGTTTACCC TCCAAAACCA 
GCATATATTG CAACTCAGTT ACCTTCATCT TCGATTAAAG GGTGCATGCT GGGCAAGACA 
TGCAGTGGGA AAACCAGCTG TCTAAAGATT TTAGAAGAAG TTTTCCATAT TGAGATACTT 
TGTATGGATA CTCTGGTTCA AGAGGCTATT CAGGCTTTCC TTAGAAATGA AACTGTAACT 
GAGGTGGAAA CACATAAAGA AGATTTATCA AAAGAAGTTC AAGATATTAA GGATTCAAAC 
ATGACGACTA TTGCAGAAAT AATTCCTACT GAGGAGAAAA AAGATGAGAA TCTACCACAA 
CAAGATGAAG TGATTATTAA ACCACCATCA GCCCAGGATG TGAGAATAAG ACTTACTAAT 
CGTGCTCAAC TTGGTTCAAA ATGTGAAAAG TTGCTCAAGA GAGGAAGAAG TATCCCCGAT 
GTACTGCTAA TTCAAATCAT GGTGAATGCT ATTAATGAAA TATCATCAGA CAAAGGATGG 
ATCTTGGATG GTTTTCCAAT GACCATAAAT CAAGCAAAAC TTCTTGAGGA AGCCCTTACA 
GGGTTCAACA CAGATCAACC AGAACATGAA ATAAAGGCTC ACAAAAAATC AACATTGGTT 
TTTGATCCAT CTGCTCCCAA AGACCCCCCT CCTCCCCCAC CTGCATTTGA TTTTGTTATG 
TTGCTGGATA TAACTGACAC ATTGGTGCTG AATCGTGTGA TGGGAGAGGA GAAAAGAAAC 
ACTGACACAA CTGGTATACC AGAAAGTGCA ACTTCAGAAA TGTTTCCTGA AGAACAAGAA 
AAGTTTGAAC TTTTGAGGGA TCAGATACAA CACAGAATTT TAGGTTTCTT GGACAACTGG 
CCAGAGCTGG AAAAATGGTT TTCAGGAAAA CAAGACATTC TAATCACAGT CAATGCAGAA 
GTAGATAAAA GGGCTTTAGC CACAAAACTA CAAGAGCTTT TTATTGCTGA ACTGAGGAAA 
AAAGAAGCTA AAGCAAAACA GGAATTAGAA GAACAGGAAG AGGAAAGGAA AAAGAAAGAA 
GCCCTCCCAC CTGAACCGCC ACCTCCACCT CCCCCTGAAC CAGAAGAAGA GCCAAAGGAA 
GCAAAAGGGA AAGGAAAAGG GAAAGGTAAA GGTGCTAAAG AAGGAAAAGG AAAGAAAGAC 
ACTTCTCCCA AGGGCAAAGA TGCTGCTAAA GAAGCAAAAG CAAAGAAAAG TGAAACTTCT 
CCAAAAGGCA AAGGAAGGTC ACCAGACAAG AGGTCACCTG AAGATAAAAC ATCACCTCCT 
GCACCTCCAG GACCACCACC AGTCAAAGCA GGATCAGAAG ACTGGGTCTA TGTGGATGAG 
CCACTTCCTC AGGAAATATT TGAATATCTA GACACTTTTT GGGAAGTAAT AGAAAGTTCA 
TACATAAATA CCAGCAAAGC TATATTCAGA CAGATAAGAG ATGAGAGACA TAGTGTACTT 
TATTATCTAC ATAATGTCAG ACTAAATTAC CAGGGATATC TGAAACGGCC AGATCACAAG 
CAGGAATTTG TATCACAATG GCAGGAGGAT TTTAACAACC TCCCTGAAGA CTTATGGGAG 
GATGAGGAAA CAAAAGCTGA ACTACACCAA AGAGTCAATG ATTTAAGGGA CCGCCTGTGG 
GATATATGTG ATACTCGAAA GGAAGAGGCA GAACAAGAAC GTGCTGATAT CATTGATGAA 
TCCTGGTTAC AAGATCATCT TGGAATTCTT ATGAATCATT TCTTTTCTTT GATGCAGGTG 
GAACTTAGCC GTTTCCAGGA CATAAAGACT TTTCTACAAG ATTACTATAG GGGAATGGAA 
GGAAAAATCC CCACCGACCT TGCTAAATCT GGATATACTC GAGTTCCCCT TATAGATCTT 
GATGAAGAGG ATACCGATAT CACCTCCAAA ATCCCTGTAA TTCCTCGGAG ACAGCCTTCT 
CCTGAAGGAC TATTAAACAA GCCCAGAAGT AAACTATCCC TGAGAGGAGG CAGACCTGAT 
TACTGCTTAG AAAATGTCCA ACTCAAATTT GAAAGAGATG AGAAGACCCT TATGACTATA 
TGGCAGAATA CATCTTCAGC CATCAACCTC TTGGTAAATT CTGAAATACA GTTGAGGAAA 
CTAGAAGATG ATAAAGAAAA CAAACCAACA TCAACTAAAG AAAAATCTCC TCAAGATGGT 
GGCAAAGGTC CTAAAGGTGC TAAGAAAAAG CCAGATAAGA AGAAAGTTCC ATCACCAGTT 
GAAGCCGTTT CTATGGTTCT AACACCAGAA GAACAGGAGG CTCTTGATAA GAGGAATGAA 
TTGAAAATGA AAATAAAGGA AGAACATATT ACAGCTCTTC AGTGTGAAGC ACAAGCTTCA 
ATATTCCGAC TGGAACTGAT CAAGATGAAG GCCTTATCTT ACATGGAAGA GCTGCTAGGA 
AGAGCAGTTG AGGTTTACAA ACTGATGGAC AAATGGCTCG GAGCCCGATA CTTAGCTGAA 
ATGGCAAGTG TGGAAAAATT AACTGAAGTG GCTCACTACC ACATTGAAAC CTCAACAAGG 
ATTCAGAATG AACTTATTTT AAATGAAGAA GATTTCTACA TCAATGGTGA TGTGAAAGTC 
TTTCCAGATC CGCTTCCTCC CGTCCGGCCT CCACCTGTGG AGAAAGAAAT GAATACTTTT 
CTCACTCTTG AACAACTCAA CACCCTTCGA AATGAGTTTC TCCAGATGGC ACCAAAAGCC 
AAAACACCAT GGAAGTATTT TAAAAAAGGA ATCTCTTCAG TTGATACTAG AAGACATGCT 
GAGGAAAAGA AAAGGCTAAG AAATCTTGAC TATTACCTAC CTAAGAGGAA CAACCAATTT 
TTGAAAATTT GGCTAAAAAA ATACCCTTGG CTCGTGTATG ATGACAGGTC AGACTTAATG 
TTCTGTGGCT TGTGTCGCAA ACATGGAGTG AGCTCAGGAG GAAACCATGT TAGCTTTTTT 
TATGGCACCA ACAACTTTAG GACTGAATTT CTCCAAGCAC ATGACAATAG TGAGGCACAC 
ACGAAGGCTG TTCTAGCAGA AGCAACAGGG GTTTCTTCCC TGACTGATGC TGCCAAGGAG 
CAGATTCTAA AGAACAGGAA CAAAGTAATC TTGGGAAGAA TAGAAAACCT CTTTAGAACA 
TGTCATGCTA TTGCCAAGAC TGGACGTCCC CTCAAAGACT TTGTCTGGAT GTGTAAACTG 
GATGATATGA AGGGTGTAGA CGTTGGCCCA ATATTCAGGA CTGACAAGTC AGCCAGAACT 
TTCATATACT TTATTGCTGA AGTAGAACGC ATATGTCTTC GAGAGAAATT AGAAAAATGT 
AAGTTCTTCT CACTCATCAG TGATGGTGGC TCAGGCAGCT TCATAGAAGA CACCAAAGTT 
GTTTACATCC AGTTTGCATA TGAGGGCAGG GTCTGCTGTC AGGTTGTTGG AGTACAGACT 
GTGGACAAAA TTGAACCGGT GGCAATAAAA AGTGCCATTG AAAAAACACT TGAGATAAAT 
CTTCAACTAA ATCTGTCTAG CAAAGACTGG TCCAAAAAAC TCGTTGGGTT TGGAAGTGAT 
GGTGCATCTG TCATGGTGGG AGAGAACAAT GGTGTAGCCA GGCTTCTGAA AGAAATTCAG 
CCATGTGTGC AAACAGTCCA CTGCTTTGCA CATAGACTTG AACTAGCCTA CCAAGAAGCA 
TTGCAGAGCA TCCAATTATA CAGTACTATG AATGATCTTC TCCAGAATAT ATATTATTTT 
TATCATGATT CACCTTCAAA TACAAAAGCT CTGATAGCTG CCTTTGAAGA CCTTAAAATC 
CGCCCTGTGA TTCCCTCTCG AGTTGGCAAC AACAGATGGC TCCCTCGGTT ACAGATGGCT 
CTACAAATCT TTCTCAAAGG ATATTCTCCA ATTGTCAAGC AATTAAGAAC AATGCATGAA 
GGCAGAAGTT CCGCTGATCA ACAGAAAGCT CAAGAGCTCC TACAGTTCCT TCTCCAAGCT 
GAGGTTGTCA GATTTGCTCA CTTCTTGCTG GATGTAATCA ACGTCCTCAG CATTTTGTCT 
CGAGTCAATC AGGACAGAAA TTCATCAATA GGAGATGTAT TTGCAACCCT ACAATCAACA 
CTAGGAACAC TCAAGATGTA TAAAAAAAGA CCAGGTCCAA AGGAACGTCT GACAGAGTCA 
GCCACACACT TTCATGGTAA CCAACTGACA GGAAATGGAA ATATCTCTGA AGTACGAAAC 
ACAGTCTTAA CAAATCTCTC AAAGCATCTT CATGATTGTT TCAGTGATGC CAGCCATGAT 
GTGGTGAGGG CGACCACTAT TGGAAGTTTC AAATTATGGC CAAGAAAGCT AGATCAAGAG 
TTTGGTGAGA AAGAGGTAAC AATTCTTACA AAGCATTATG AACCTGTGTT GGAAGGGGCC 
AATGTGAACA TTGATGAAGT GGACACAGAA TGGAGCATGA TGAAATTAGA GATTTATAAT 
CGATTTCAGA ACATCCGAAA GTTAACCTGG GATATTGTGA ATTCCATCTA CTCATACAAG 
TATCCCAATA TATTGACCCT AGTGGATCTG GTGCTGACAC TCCCTGCAAG TTCAGCTGAG 
GCGGAACGTG GGTTCAGTCA GATGAAACTA GCCAAGTCAC AAATGCATTT TAAAACAGAT 
CCTGAGAACA TGACAGACAT TTTAATAATC CAGTTGAATT CTCCAGACAT CAATAAGTTT 
GATCCCAAGA AAGCCATCCA TCTATGGAAT ACTTCTTGGC AGAGAAATAC ACTTCCACCA 
ATTGACCACA CAATAACCAA GTCAGATTGC TCCTCTAGCT CAGATAGTCT TTATGAAAGT 
GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_016431612.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016287098.1 Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X7, mRNA.

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CloneID	OMc41047
Clone ID Related Accession (Same CDS sequence)	XM_016431612.1
Accession Version	XM_016431612.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	sperm flagellar protein 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008803.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACA TCCTGTGCAG GTGGCTAAAC CAGGAGGTGA AGCTGTCCCG GACAGTGAGT 
TCAAAGGGGA TTGAAAAAGA TTTTTCTAAT GGCTTCCTAC TGGGTGAACT TCTGCACAAA 
TATGAACTTC AGGATGACTT TCCTCTGTTT TCAGAAGGCA GGGTTTCCAA TGCAAAACTT 
AATAATTTTT CTCGCTTAGA ACCAACATTT CAACTCCTGG GTGTTCAGTT CGATCAAAAT 
GTGGCCCAGA ATATTATGAC AGAAAAGCAT GGCTCTGTAA CCATACTTCT TTATCAGTTG 
TACATTGCGC TTCAAAAAAA GAGAAAAAGT GGACTCACGG GAGTAGCAAT GCAAACAATG 
AGACCTTTAG CACCTGCAAA GCTACAAACT ATGAGAAGTG AGATTTATCG AGAGAGACTT 
AAGAACCTGG TGCCTCGGCA AACTGATTTG AATCTAAGTC GGGTTTCAGA AAAGTTTGAA 
GCAAAGTATA AAAAAATGGA AGAAAAAATT GCTCGTGTTC ATTTTCAAAA TATCCGAAAA 
TTTGAAAAGT TCCAGGAAGA ACAAAGAATT CAAGATCTTG AAAAGCAGCG CCTGGGAAGA 
AGACGACAAC ATGAAATGAT GGCTAGAATC CAAGCAGCCA TTTTACAGAT TCCCAAAACA 
GTACCCAATC GTACTCTGAA ATCTATTGAG AGCCACAAAA TGATGATGAA GAAAAAAGAG 
GAAGAAGATG TGCTGAATGA AATTCAGAAG TTTGAAATGC TAATAAAAAA GGAGCTTGAG 
GCCAAAGGCA GTGGATCAAA AGTTCCTTCG GCAGCCCAGA CAACTGCTGA CTTGTTAAGC 
ACGTATTCAG ATGAGGAATA CATTAAAAAA ATTCAAAAAC GTCTAGAGGA AGATACCTTA 
GCCCGGGAAC AAAGGGAGAA AAGACGCAGA AAATTGTTAA TGGATCAATT GATAGCCCAT 
GAAGCTCAAG AGGAGGCTTA CCGAGAAGAA CAACTCATTA ACCGACTAAT GAGACAATCT 
CAGCAGGAAC GTCGGATTGC TGTGCAACTC ATGCATGTTC GACACGAAAA GGAAGTTTTA 
TGGCAAAACA GGGTTTTCAG AGAAAAACAA TATGAAGAAA GACGGCTGAA AGATTTCCAG 
GATGCTCTTG ACAGAGAAGC AGCTCTTGCA AGACAAGCCA AGCTGGAGTA TGAAGAACAA 
GCCCAAAAGG AAATAGAAAT TCATGAAAGA ATAGCAGCAG AAAGATCTCA AGCTCGTTAT 
AAAAAGCATT ATTCAATGTG CACAGAAATC TTGGACAATA TCATTGATTT ATCAACTAAA 
ATAGCAGAAT ATCGAGAATT AACACATAAT CTTATTCCAG TTAAAATTAT GCGTGATTGG 
AAGGAGCTGT TTTTCAGTGG AAAGCCAATT TATGAAATAG CCTCCATTGA CTCCAAGCCA 
TCTCGTTTTA CCTCAGAACA ACTAATTGAG CTATCCAAAA GGAATTTACT AGATGACAAT 
GATTATGAGG AGTACAAGAA CTTGACAGGA GAGTGGGTCC TCCCAGAAGA TATGGCTGAT 
GTTCCCCTAC CTTCCAACAA TAACATATTG GGTCATGTGA TTCGAGTAGT TCATGAAAAA 
GTTTACCCTC CAAAACCAGC ATATATTGCA ACTCAGTTAC CTTCATCTTC GATTAAAGGG 
TGCATGCTGG GCAAGACATG CAGTGGGAAA ACCAGCTGTC TAAAGATTTT AGAAGAAGTT 
TTCCATATTG AGATACTTTG TATGGATACT CTGGTTCAAG AGGCTATTCA GGCTTTCCTT 
AGAAATGAAA CTGTAACTGA GGTGGAAACA CATAAAGAAG ATTTATCAAA AGAAGTTCAA 
GATATTAAGG ATTCAAACAT GACGACTATT GCAGAAATAA TTCCTACTGA GGAGAAAAAA 
GATGAGAATC TACCACAACA AGATGAAGTG ATTATTAAAC CACCATCAGC CCAGGATGTG 
AGAATAAGAC TTACTAATCG TGCTCAACTT GGTTCAAAAT GTGAAAAGTT GCTCAAGAGA 
GGAAGAAGTA TCCCCGATGT ACTGCTAATT CAAATCATGG TGAATGCTAT TAATGAAATA 
TCATCAGACA AAGGATGGAT CTTGGATGGT TTTCCAATGA CCATAAATCA AGCAAAACTT 
CTTGAGGAAG CCCTTACAGG GTTCAACACA GATCAACCAG AACATGAAAT AAAGGCTCAC 
AAAAAATCAA CATTGGTTTT TGATCCATCT GCTCCCAAAG ACCCCCCTCC TCCCCCACCT 
GCATTTGATT TTGTTATGTT GCTGGATATA ACTGACACAT TGGTGCTGAA TCGTGTGATG 
GGAGAGGAGA AAAGAAACAC TGACACAACT GGTATACCAG AAAGTGCAAC TTCAGAAATG 
TTTCCTGAAG AACAAGAAAA GTTTGAACTT TTGAGGGATC AGATACAACA CAGAATTTTA 
GGTTTCTTGG ACAACTGGCC AGAGCTGGAA AAATGGTTTT CAGGAAAACA AGACATTCTA 
ATCACAGTCA ATGCAGAAGT AGATAAAAGG GCTTTAGCCA CAAAACTACA AGAGCTTTTT 
ATTGCTGAAC TGAGGAAAAA AGAAGCTAAA GCAAAACAGG AATTAGAAGA ACAGGAAGAG 
GAAAGGAAAA AGAAAGAAGC CCTCCCACCT GAACCGCCAC CTCCACCTCC CCCTGAACCA 
GAAGAAGAGC CAAAGGAAGC AAAAGGGAAA GGAAAAGGGA AAGGTAAAGG TGCTAAAGAA 
GGAAAAGGAA AGAAAGACAC TTCTCCCAAG GGCAAAGATG CTGCTAAAGA AGCAAAAGCA 
AAGAAAAGTG AAACTTCTCC AAAAGGCAAA GGAAGGTCAC CAGACAAGAG GTCACCTGAA 
GATAAAACAT CACCTCCTGC ACCTCCAGGA CCACCACCAG TCAAAGCAGG ATCAGAAGAC 
TGGGTCTATG TGGATGAGCC ACTTCCTCAG GAAATATTTG AATATCTAGA CACTTTTTGG 
GAAGTAATAG AAAGTTCATA CATAAATACC AGCAAAGCTA TATTCAGACA GATAAGAGAT 
GAGAGACATA GTGTACTTTA TTATCTACAT AATGTCAGAC TAAATTACCA GGGATATCTG 
AAACGGCCAG ATCACAAGCA GGAATTTGTA TCACAATGGC AGGAGGATTT TAACAACCTC 
CCTGAAGACT TATGGGAGGA TGAGGAAACA AAAGCTGAAC TACACCAAAG AGTCAATGAT 
TTAAGGGACC GCCTGTGGGA TATATGTGAT ACTCGAAAGG AAGAGGCAGA ACAAGAACGT 
GCTGATATCA TTGATGAATC CTGGTTACAA GATCATCTTG GAATTCTTAT GAATCATTTC 
TTTTCTTTGA TGCAGGTGGA ACTTAGCCGT TTCCAGGACA TAAAGACTTT TCTACAAGAT 
TACTATAGGG GAATGGAAGG AAAAATCCCC ACCGACCTTG CTAAATCTGG ATATACTCGA 
GTTCCCCTTA TAGATCTTGA TGAAGAGGAT ACCGATATCA CCTCCAAAAT CCCTGTAATT 
CCTCGGAGAC AGCCTTCTCC TGAAGGACTA TTAAACAAGC CCAGAAGTAA ACTATCCCTG 
AGAGGAGGCA GACCTGATTA CTGCTTAGAA AATGTCCAAC TCAAATTTGA AAGAGATGAG 
AAGACCCTTA TGACTATATG GCAGAATACA TCTTCAGCCA TCAACCTCTT GGTAAATTCT 
GAAATACAGT TGAGGAAACT AGAAGATGAT AAAGAAAACA AACCAACATC AACTAAAGAA 
AAATCTCCTC AAGATGGTGG CAAAGGTCCT AAAGGTGCTA AGAAAAAGCC AGATAAGAAG 
AAAGTTCCAT CACCAGTTGA AGCCGTTTCT ATGGTTCTAA CACCAGAAGA ACAGGAGGCT 
CTTGATAAGA GGAATGAATT GAAAATGAAA ATAAAGGAAG AACATATTAC AGCTCTTCAG 
TGTGAAGCAC AAGCTTCAAT ATTCCGACTG GAACTGATCA AGATGAAGGC CTTATCTTAC 
ATGGAAGAGC TGCTAGGAAG AGCAGTTGAG GTTTACAAAC TGATGGACAA ATGGCTCGGA 
GCCCGATACT TAGCTGAAAT GGCAAGTGTG GAAAAATTAA CTGAAGTGGC TCACTACCAC 
ATTGAAACCT CAACAAGGAT TCAGAATGAA CTTATTTTAA ATGAAGAAGA TTTCTACATC 
AATGGTGATG TGAAAGTCTT TCCAGATCCG CTTCCTCCCG TCCGGCCTCC ACCTGTGGAG 
AAAGAAATGA ATACTTTTCT CACTCTTGAA CAACTCAACA CCCTTCGAAA TGAGTTTCTC 
CAGATGGCAC CAAAAGGTTA TTTGGGAAGT AAACATTTCA CTGATATTTT GTCTGACTTG 
GTGACTTTGA ACCTTGGAAC AAACCACATT CCCAAGGCTT GGCTGCACCT CATTCTTTCT 
GAATTACAAG AGCTAACATC TTTATTAGTA GTGAATTCAG AGTTTGTTGA CTGGCGCAGA 
TTTTTATTAG TAGTTTCACA GCCTTGGCCA TTGCCATCTG AGGAAGAGCT TCTTGAGACA 
TTGAACAGAT TTAAAGCAAT TGATGAAGAT GAAAAAGGCA CAGTAACCTG GGAGCAGTAT 
GAGCAGGTTG GTTTGTGGTT TACAGGTGAT TTAAATATTG AAGTTCCAGA AGACCCAAAT 
GAACCTTTGC CATATAATAG ACCAGCACAT CTTATAATGT TTTTCTTTGA CTTGTTTGCG 
GACTTCCAGA AAGATCCACC AGAACTGGAC TATCTTAACA TGCTTCTTTA CTTTGCATGT 
CATCCAAATC CTGTGGAAGG GGTCTACAGA GCCCTGAGCC TAGCTATCGG GACCCATGTC 
TTTCGAAAAT ATGAAACGAT AGCTGTGCCT ATGGAATATG TGACGCCTGC CTACTATACT 
GACACAACAA TCGAGGAACT TCCTGAAGTA GAAGAAGTAG TAAAAGAAGA TAAAGAAAAA 
ATAATAGTTC CAGAATTTTC TAATAAAGAG ACTATTTCCA TTCCAATGCT ACTAAAAATA 
TTCCAAAGAA AAAAGGAAAC ACAAGATAAC CACCGGTTTT TTGAGGAGGA AAAGCAAGAG 
AATGTTTTTT CTGAGGTTTT CCAAAAAGTA TATCGGGTTG TAGGAAGCAA GAATTTGGAG 
CCAGTTGAAG TTGCTGTTCT TCTGAAGCAC CCTTTTATGC AGGATTTAAT TTCAACTTAT 
AAAGAATACA AATTTCCAGA TATTAAAGGA ATTCTTCAAC GAGTTGACCA ACTGCAAGTC 
CTCACAAATG GGGACCAGAA AACTACTGAG GAAAAGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016287098.1
CDS	80..5401
Translation	MSDILCRWLNQEVKLSRTVSSKGIEKDFSNGFLLGELLHKYELQDDFPLFSEGRVSNAKLNNFSRLEPTFQLLGVQFDQNVAQNIMTEKHGSVTILLYQLYIALQKKRKSGLTGVAMQTMRPLAPAKLQTMRSEIYRERLKNLVPRQTDLNLSRVSEKFEAKYKKMEEKIARVHFQNIRKFEKFQEEQRIQDLEKQRLGRRRQHEMMARIQAAILQIPKTVPNRTLKSIESHKMMMKKKEEEDVLNEIQKFEMLIKKELEAKGSGSKVPSAAQTTADLLSTYSDEEYIKKIQKRLEEDTLAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRVFREKQYEERRLKDFQDALDREAALARQAKLEYEEQAQKEIEIHERIAAERSQARYKKHYSMCTEILDNIIDLSTKIAEYRELTHNLIPVKIMRDWKELFFSGKPIYEIASIDSKPSRFTSEQLIELSKRNLLDDNDYEEYKNLTGEWVLPEDMADVPLPSNNNILGHVIRVVHEKVYPPKPAYIATQLPSSSIKGCMLGKTCSGKTSCLKILEEVFHIEILCMDTLVQEAIQAFLRNETVTEVETHKEDLSKEVQDIKDSNMTTIAEIIPTEEKKDENLPQQDEVIIKPPSAQDVRIRLTNRAQLGSKCEKLLKRGRSIPDVLLIQIMVNAINEISSDKGWILDGFPMTINQAKLLEEALTGFNTDQPEHEIKAHKKSTLVFDPSAPKDPPPPPPAFDFVMLLDITDTLVLNRVMGEEKRNTDTTGIPESATSEMFPEEQEKFELLRDQIQHRILGFLDNWPELEKWFSGKQDILITVNAEVDKRALATKLQELFIAELRKKEAKAKQELEEQEEERKKKEALPPEPPPPPPPEPEEEPKEAKGKGKGKGKGAKEGKGKKDTSPKGKDAAKEAKAKKSETSPKGKGRSPDKRSPEDKTSPPAPPGPPPVKAGSEDWVYVDEPLPQEIFEYLDTFWEVIESSYINTSKAIFRQIRDERHSVLYYLHNVRLNYQGYLKRPDHKQEFVSQWQEDFNNLPEDLWEDEETKAELHQRVNDLRDRLWDICDTRKEEAEQERADIIDESWLQDHLGILMNHFFSLMQVELSRFQDIKTFLQDYYRGMEGKIPTDLAKSGYTRVPLIDLDEEDTDITSKIPVIPRRQPSPEGLLNKPRSKLSLRGGRPDYCLENVQLKFERDEKTLMTIWQNTSSAINLLVNSEIQLRKLEDDKENKPTSTKEKSPQDGGKGPKGAKKKPDKKKVPSPVEAVSMVLTPEEQEALDKRNELKMKIKEEHITALQCEAQASIFRLELIKMKALSYMEELLGRAVEVYKLMDKWLGARYLAEMASVEKLTEVAHYHIETSTRIQNELILNEEDFYINGDVKVFPDPLPPVRPPPVEKEMNTFLTLEQLNTLRNEFLQMAPKGYLGSKHFTDILSDLVTLNLGTNHIPKAWLHLILSELQELTSLLVVNSEFVDWRRFLLVVSQPWPLPSEEELLETLNRFKAIDEDEKGTVTWEQYEQVGLWFTGDLNIEVPEDPNEPLPYNRPAHLIMFFFDLFADFQKDPPELDYLNMLLYFACHPNPVEGVYRALSLAIGTHVFRKYETIAVPMEYVTPAYYTDTTIEELPEVEEVVKEDKEKIIVPEFSNKETISIPMLLKIFQRKKETQDNHRFFEEEKQENVFSEVFQKVYRVVGSKNLEPVEVAVLLKHPFMQDLISTYKEYKFPDIKGILQRVDQLQVLTNGDQKTTEEKK

Target ORF information:

RefSeq Version	XM_016431612.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016431612.1

ORF Insert Sequence:

ATGTCGGACA TCCTGTGCAG GTGGCTAAAC CAGGAGGTGA AGCTGTCCCG GACAGTGAGT 
TCAAAGGGGA TTGAAAAAGA TTTTTCTAAT GGCTTCCTAC TGGGTGAACT TCTGCACAAA 
TATGAACTTC AGGATGACTT TCCTCTGTTT TCAGAAGGCA GGGTTTCCAA TGCAAAACTT 
AATAATTTTT CTCGCTTAGA ACCAACATTT CAACTCCTGG GTGTTCAGTT CGATCAAAAT 
GTGGCCCAGA ATATTATGAC AGAAAAGCAT GGCTCTGTAA CCATACTTCT TTATCAGTTG 
TACATTGCGC TTCAAAAAAA GAGAAAAAGT GGACTCACGG GAGTAGCAAT GCAAACAATG 
AGACCTTTAG CACCTGCAAA GCTACAAACT ATGAGAAGTG AGATTTATCG AGAGAGACTT 
AAGAACCTGG TGCCTCGGCA AACTGATTTG AATCTAAGTC GGGTTTCAGA AAAGTTTGAA 
GCAAAGTATA AAAAAATGGA AGAAAAAATT GCTCGTGTTC ATTTTCAAAA TATCCGAAAA 
TTTGAAAAGT TCCAGGAAGA ACAAAGAATT CAAGATCTTG AAAAGCAGCG CCTGGGAAGA 
AGACGACAAC ATGAAATGAT GGCTAGAATC CAAGCAGCCA TTTTACAGAT TCCCAAAACA 
GTACCCAATC GTACTCTGAA ATCTATTGAG AGCCACAAAA TGATGATGAA GAAAAAAGAG 
GAAGAAGATG TGCTGAATGA AATTCAGAAG TTTGAAATGC TAATAAAAAA GGAGCTTGAG 
GCCAAAGGCA GTGGATCAAA AGTTCCTTCG GCAGCCCAGA CAACTGCTGA CTTGTTAAGC 
ACGTATTCAG ATGAGGAATA CATTAAAAAA ATTCAAAAAC GTCTAGAGGA AGATACCTTA 
GCCCGGGAAC AAAGGGAGAA AAGACGCAGA AAATTGTTAA TGGATCAATT GATAGCCCAT 
GAAGCTCAAG AGGAGGCTTA CCGAGAAGAA CAACTCATTA ACCGACTAAT GAGACAATCT 
CAGCAGGAAC GTCGGATTGC TGTGCAACTC ATGCATGTTC GACACGAAAA GGAAGTTTTA 
TGGCAAAACA GGGTTTTCAG AGAAAAACAA TATGAAGAAA GACGGCTGAA AGATTTCCAG 
GATGCTCTTG ACAGAGAAGC AGCTCTTGCA AGACAAGCCA AGCTGGAGTA TGAAGAACAA 
GCCCAAAAGG AAATAGAAAT TCATGAAAGA ATAGCAGCAG AAAGATCTCA AGCTCGTTAT 
AAAAAGCATT ATTCAATGTG CACAGAAATC TTGGACAATA TCATTGATTT ATCAACTAAA 
ATAGCAGAAT ATCGAGAATT AACACATAAT CTTATTCCAG TTAAAATTAT GCGTGATTGG 
AAGGAGCTGT TTTTCAGTGG AAAGCCAATT TATGAAATAG CCTCCATTGA CTCCAAGCCA 
TCTCGTTTTA CCTCAGAACA ACTAATTGAG CTATCCAAAA GGAATTTACT AGATGACAAT 
GATTATGAGG AGTACAAGAA CTTGACAGGA GAGTGGGTCC TCCCAGAAGA TATGGCTGAT 
GTTCCCCTAC CTTCCAACAA TAACATATTG GGTCATGTGA TTCGAGTAGT TCATGAAAAA 
GTTTACCCTC CAAAACCAGC ATATATTGCA ACTCAGTTAC CTTCATCTTC GATTAAAGGG 
TGCATGCTGG GCAAGACATG CAGTGGGAAA ACCAGCTGTC TAAAGATTTT AGAAGAAGTT 
TTCCATATTG AGATACTTTG TATGGATACT CTGGTTCAAG AGGCTATTCA GGCTTTCCTT 
AGAAATGAAA CTGTAACTGA GGTGGAAACA CATAAAGAAG ATTTATCAAA AGAAGTTCAA 
GATATTAAGG ATTCAAACAT GACGACTATT GCAGAAATAA TTCCTACTGA GGAGAAAAAA 
GATGAGAATC TACCACAACA AGATGAAGTG ATTATTAAAC CACCATCAGC CCAGGATGTG 
AGAATAAGAC TTACTAATCG TGCTCAACTT GGTTCAAAAT GTGAAAAGTT GCTCAAGAGA 
GGAAGAAGTA TCCCCGATGT ACTGCTAATT CAAATCATGG TGAATGCTAT TAATGAAATA 
TCATCAGACA AAGGATGGAT CTTGGATGGT TTTCCAATGA CCATAAATCA AGCAAAACTT 
CTTGAGGAAG CCCTTACAGG GTTCAACACA GATCAACCAG AACATGAAAT AAAGGCTCAC 
AAAAAATCAA CATTGGTTTT TGATCCATCT GCTCCCAAAG ACCCCCCTCC TCCCCCACCT 
GCATTTGATT TTGTTATGTT GCTGGATATA ACTGACACAT TGGTGCTGAA TCGTGTGATG 
GGAGAGGAGA AAAGAAACAC TGACACAACT GGTATACCAG AAAGTGCAAC TTCAGAAATG 
TTTCCTGAAG AACAAGAAAA GTTTGAACTT TTGAGGGATC AGATACAACA CAGAATTTTA 
GGTTTCTTGG ACAACTGGCC AGAGCTGGAA AAATGGTTTT CAGGAAAACA AGACATTCTA 
ATCACAGTCA ATGCAGAAGT AGATAAAAGG GCTTTAGCCA CAAAACTACA AGAGCTTTTT 
ATTGCTGAAC TGAGGAAAAA AGAAGCTAAA GCAAAACAGG AATTAGAAGA ACAGGAAGAG 
GAAAGGAAAA AGAAAGAAGC CCTCCCACCT GAACCGCCAC CTCCACCTCC CCCTGAACCA 
GAAGAAGAGC CAAAGGAAGC AAAAGGGAAA GGAAAAGGGA AAGGTAAAGG TGCTAAAGAA 
GGAAAAGGAA AGAAAGACAC TTCTCCCAAG GGCAAAGATG CTGCTAAAGA AGCAAAAGCA 
AAGAAAAGTG AAACTTCTCC AAAAGGCAAA GGAAGGTCAC CAGACAAGAG GTCACCTGAA 
GATAAAACAT CACCTCCTGC ACCTCCAGGA CCACCACCAG TCAAAGCAGG ATCAGAAGAC 
TGGGTCTATG TGGATGAGCC ACTTCCTCAG GAAATATTTG AATATCTAGA CACTTTTTGG 
GAAGTAATAG AAAGTTCATA CATAAATACC AGCAAAGCTA TATTCAGACA GATAAGAGAT 
GAGAGACATA GTGTACTTTA TTATCTACAT AATGTCAGAC TAAATTACCA GGGATATCTG 
AAACGGCCAG ATCACAAGCA GGAATTTGTA TCACAATGGC AGGAGGATTT TAACAACCTC 
CCTGAAGACT TATGGGAGGA TGAGGAAACA AAAGCTGAAC TACACCAAAG AGTCAATGAT 
TTAAGGGACC GCCTGTGGGA TATATGTGAT ACTCGAAAGG AAGAGGCAGA ACAAGAACGT 
GCTGATATCA TTGATGAATC CTGGTTACAA GATCATCTTG GAATTCTTAT GAATCATTTC 
TTTTCTTTGA TGCAGGTGGA ACTTAGCCGT TTCCAGGACA TAAAGACTTT TCTACAAGAT 
TACTATAGGG GAATGGAAGG AAAAATCCCC ACCGACCTTG CTAAATCTGG ATATACTCGA 
GTTCCCCTTA TAGATCTTGA TGAAGAGGAT ACCGATATCA CCTCCAAAAT CCCTGTAATT 
CCTCGGAGAC AGCCTTCTCC TGAAGGACTA TTAAACAAGC CCAGAAGTAA ACTATCCCTG 
AGAGGAGGCA GACCTGATTA CTGCTTAGAA AATGTCCAAC TCAAATTTGA AAGAGATGAG 
AAGACCCTTA TGACTATATG GCAGAATACA TCTTCAGCCA TCAACCTCTT GGTAAATTCT 
GAAATACAGT TGAGGAAACT AGAAGATGAT AAAGAAAACA AACCAACATC AACTAAAGAA 
AAATCTCCTC AAGATGGTGG CAAAGGTCCT AAAGGTGCTA AGAAAAAGCC AGATAAGAAG 
AAAGTTCCAT CACCAGTTGA AGCCGTTTCT ATGGTTCTAA CACCAGAAGA ACAGGAGGCT 
CTTGATAAGA GGAATGAATT GAAAATGAAA ATAAAGGAAG AACATATTAC AGCTCTTCAG 
TGTGAAGCAC AAGCTTCAAT ATTCCGACTG GAACTGATCA AGATGAAGGC CTTATCTTAC 
ATGGAAGAGC TGCTAGGAAG AGCAGTTGAG GTTTACAAAC TGATGGACAA ATGGCTCGGA 
GCCCGATACT TAGCTGAAAT GGCAAGTGTG GAAAAATTAA CTGAAGTGGC TCACTACCAC 
ATTGAAACCT CAACAAGGAT TCAGAATGAA CTTATTTTAA ATGAAGAAGA TTTCTACATC 
AATGGTGATG TGAAAGTCTT TCCAGATCCG CTTCCTCCCG TCCGGCCTCC ACCTGTGGAG 
AAAGAAATGA ATACTTTTCT CACTCTTGAA CAACTCAACA CCCTTCGAAA TGAGTTTCTC 
CAGATGGCAC CAAAAGGTTA TTTGGGAAGT AAACATTTCA CTGATATTTT GTCTGACTTG 
GTGACTTTGA ACCTTGGAAC AAACCACATT CCCAAGGCTT GGCTGCACCT CATTCTTTCT 
GAATTACAAG AGCTAACATC TTTATTAGTA GTGAATTCAG AGTTTGTTGA CTGGCGCAGA 
TTTTTATTAG TAGTTTCACA GCCTTGGCCA TTGCCATCTG AGGAAGAGCT TCTTGAGACA 
TTGAACAGAT TTAAAGCAAT TGATGAAGAT GAAAAAGGCA CAGTAACCTG GGAGCAGTAT 
GAGCAGGTTG GTTTGTGGTT TACAGGTGAT TTAAATATTG AAGTTCCAGA AGACCCAAAT 
GAACCTTTGC CATATAATAG ACCAGCACAT CTTATAATGT TTTTCTTTGA CTTGTTTGCG 
GACTTCCAGA AAGATCCACC AGAACTGGAC TATCTTAACA TGCTTCTTTA CTTTGCATGT 
CATCCAAATC CTGTGGAAGG GGTCTACAGA GCCCTGAGCC TAGCTATCGG GACCCATGTC 
TTTCGAAAAT ATGAAACGAT AGCTGTGCCT ATGGAATATG TGACGCCTGC CTACTATACT 
GACACAACAA TCGAGGAACT TCCTGAAGTA GAAGAAGTAG TAAAAGAAGA TAAAGAAAAA 
ATAATAGTTC CAGAATTTTC TAATAAAGAG ACTATTTCCA TTCCAATGCT ACTAAAAATA 
TTCCAAAGAA AAAAGGAAAC ACAAGATAAC CACCGGTTTT TTGAGGAGGA AAAGCAAGAG 
AATGTTTTTT CTGAGGTTTT CCAAAAAGTA TATCGGGTTG TAGGAAGCAA GAATTTGGAG 
CCAGTTGAAG TTGCTGTTCT TCTGAAGCAC CCTTTTATGC AGGATTTAAT TTCAACTTAT 
AAAGAATACA AATTTCCAGA TATTAAAGGA ATTCTTCAAC GAGTTGACCA ACTGCAAGTC 
CTCACAAATG GGGACCAGAA AACTACTGAG GAAAAGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_016431613.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016287099.1 Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X8, mRNA.

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CloneID	OMc41048
Clone ID Related Accession (Same CDS sequence)	XM_016431613.1
Accession Version	XM_016431613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	sperm flagellar protein 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008803.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGATT GGAAGGAGCT GTTTTTCAGT GGAAAGCCAA TTTATGAAAT AGCCTCCATT 
GACTCCAAGC CATCTCGTTT TACCTCAGAA CAACTAATTG AGCTATCCAA AAGGAATTTA 
CTAGATGACA ATGATTATGA GGAGTACAAG AACTTGACAG GAGAGTGGGT CCTCCCAGAA 
GATATGGCTG ATGTTCCCCT ACCTTCCAAC AATAACATAT TGGGTCATGT GATTCGAGTA 
GTTCATGAAA AAGTTTACCC TCCAAAACCA GCATATATTG CAACTCAGTT ACCTTCATCT 
TCGATTAAAG GGTGCATGCT GGGCAAGACA TGCAGTGGGA AAACCAGCTG TCTAAAGATT 
TTAGAAGAAG TTTTCCATAT TGAGATACTT TGTATGGATA CTCTGGTTCA AGAGGCTATT 
CAGGCTTTCC TTAGAAATGA AACTGTAACT GAGGTGGAAA CACATAAAGA AGATTTATCA 
AAAGAAGTTC AAGATATTAA GGATTCAAAC ATGACGACTA TTGCAGAAAT AATTCCTACT 
GAGGAGAAAA AAGATGAGAA TCTACCACAA CAAGATGAAG TGATTATTAA ACCACCATCA 
GCCCAGGATG TGAGAATAAG ACTTACTAAT CGTGCTCAAC TTGGTTCAAA ATGTGAAAAG 
TTGCTCAAGA GAGGAAGAAG TATCCCCGAT GTACTGCTAA TTCAAATCAT GGTGAATGCT 
ATTAATGAAA TATCATCAGA CAAAGGATGG ATCTTGGATG GTTTTCCAAT GACCATAAAT 
CAAGCAAAAC TTCTTGAGGA AGCCCTTACA GGGTTCAACA CAGATCAACC AGAACATGAA 
ATAAAGGCTC ACAAAAAATC AACATTGGTT TTTGATCCAT CTGCTCCCAA AGACCCCCCT 
CCTCCCCCAC CTGCATTTGA TTTTGTTATG TTGCTGGATA TAACTGACAC ATTGGTGCTG 
AATCGTGTGA TGGGAGAGGA GAAAAGAAAC ACTGACACAA CTGGTATACC AGAAAGTGCA 
ACTTCAGAAA TGTTTCCTGA AGAACAAGAA AAGTTTGAAC TTTTGAGGGA TCAGATACAA 
CACAGAATTT TAGGTTTCTT GGACAACTGG CCAGAGCTGG AAAAATGGTT TTCAGGAAAA 
CAAGACATTC TAATCACAGT CAATGCAGAA GTAGATAAAA GGGCTTTAGC CACAAAACTA 
CAAGAGCTTT TTATTGCTGA ACTGAGGAAA AAAGAAGCTA AAGCAAAACA GGAATTAGAA 
GAACAGGAAG AGGAAAGGAA AAAGAAAGAA GCCCTCCCAC CTGAACCGCC ACCTCCACCT 
CCCCCTGAAC CAGAAGAAGA GCCAAAGGAA GCAAAAGGGA AAGGAAAAGG GAAAGGTAAA 
GGTGCTAAAG AAGGAAAAGG AAAGAAAGAC ACTTCTCCCA AGGGCAAAGA TGCTGCTAAA 
GAAGCAAAAG CAAAGAAAAG TGAAACTTCT CCAAAAGGCA AAGGAAGGTC ACCAGACAAG 
AGGTCACCTG AAGATAAAAC ATCACCTCCT GCACCTCCAG GACCACCACC AGTCAAAGCA 
GGATCAGAAG ACTGGGTCTA TGTGGATGAG CCACTTCCTC AGGAAATATT TGAATATCTA 
GACACTTTTT GGGAAGTAAT AGAAAGTTCA TACATAAATA CCAGCAAAGC TATATTCAGA 
CAGATAAGAG ATGAGAGACA TAGTGTACTT TATTATCTAC ATAATGTCAG ACTAAATTAC 
CAGGGATATC TGAAACGGCC AGATCACAAG CAGGAATTTG TATCACAATG GCAGGAGGAT 
TTTAACAACC TCCCTGAAGA CTTATGGGAG GATGAGGAAA CAAAAGCTGA ACTACACCAA 
AGAGTCAATG ATTTAAGGGA CCGCCTGTGG GATATATGTG ATACTCGAAA GGAAGAGGCA 
GAACAAGAAC GTGCTGATAT CATTGATGAA TCCTGGTTAC AAGATCATCT TGGAATTCTT 
ATGAATCATT TCTTTTCTTT GATGCAGGTG GAACTTAGCC GTTTCCAGGA CATAAAGACT 
TTTCTACAAG ATTACTATAG GGGAATGGAA GGAAAAATCC CCACCGACCT TGCTAAATCT 
GGATATACTC GAGTTCCCCT TATAGATCTT GATGAAGAGG ATACCGATAT CACCTCCAAA 
ATCCCTGTAA TTCCTCGGAG ACAGCCTTCT CCTGAAGGAC TATTAAACAA GCCCAGAAGT 
AAACTATCCC TGAGAGGAGG CAGACCTGAT TACTGCTTAG AAAATGTCCA ACTCAAATTT 
GAAAGAGATG AGAAGACCCT TATGACTATA TGGCAGAATA CATCTTCAGC CATCAACCTC 
TTGGTAAATT CTGAAATACA GTTGAGGAAA CTAGAAGATG ATAAAGAAAA CAAACCAACA 
TCAACTAAAG AAAAATCTCC TCAAGATGGT GGCAAAGGTC CTAAAGGTGC TAAGAAAAAG 
CCAGATAAGA AGAAAGTTCC ATCACCAGTT GAAGCCGTTT CTATGGTTCT AACACCAGAA 
GAACAGGAGG CTCTTGATAA GAGGAATGAA TTGAAAATGA AAATAAAGGA AGAACATATT 
ACAGCTCTTC AGTGTGAAGC ACAAGCTTCA ATATTCCGAC TGGAACTGAT CAAGATGAAG 
GCCTTATCTT ACATGGAAGA GCTGCTAGGA AGAGCAGTTG AGGTTTACAA ACTGATGGAC 
AAATGGCTCG GAGCCCGATA CTTAGCTGAA ATGGCAAGTG TGGAAAAATT AACTGAAGTG 
GCTCACTACC ACATTGAAAC CTCAACAAGG ATTCAGAATG AACTTATTTT AAATGAAGAA 
GATTTCTACA TCAATGGTGA TGTGAAAGTC TTTCCAGATC CGCTTCCTCC CGTCCGGCCT 
CCACCTGTGG AGAAAGAAAT GAATACTTTT CTCACTCTTG AACAACTCAA CACCCTTCGA 
AATGAGTTTC TCCAGATGGC ACCAAAAGCC AAAACACCAT GGAAGTATTT TAAAAAAGGA 
ATCTCTTCAG TTGATACTAG AAGACATGCT GAGGAAAAGA AAAGGCTAAG AAATCTTGAC 
TATTACCTAC CTAAGAGGAA CAACCAATTT TTGAAAATTT GGCTAAAAAA ATACCCTTGG 
CTCGTGTATG ATGACAGGTC AGACTTAATG TTCTGTGGCT TGTGTCGCAA ACATGGAGTG 
AGCTCAGGAG GAAACCATGT TAGCTTTTTT TATGGCACCA ACAACTTTAG GACTGAATTT 
CTCCAAGCAC ATGACAATAG TGAGGCACAC ACGAAGGCTG TTCTAGCAGA AGCAACAGGG 
GTTTCTTCCC TGACTGATGC TGCCAAGGAG CAGATTCTAA AGAACAGGAA CAAAGTAATC 
TTGGGAAGAA TAGAAAACCT CTTTAGAACA TGTCATGCTA TTGCCAAGAC TGGACGTCCC 
CTCAAAGACT TTGTCTGGAT GTGTAAACTG GATGATATGA AGGGTGTAGA CGTTGGCCCA 
ATATTCAGGA CTGACAAGTC AGCCAGAACT TTCATATACT TTATTGCTGA AGTAGAACGC 
ATATGTCTTC GAGAGAAATT AGAAAAATGT AAGTTCTTCT CACTCATCAG TGATGGTGGC 
TCAGGCAGCT TCATAGAAGA CACCAAAGTT GTTTACATCC AGTTTGCATA TGAGGGCAGG 
GTCTGCTGTC AGGTTGTTGG AGTACAGACT GTGGACAAAA TTGAACCGGT GGCAATAAAA 
AGTGCCATTG AAAAAACACT TGAGATAAAT CTTCAACTAA ATCTGTCTAG CAAAGACTGG 
TCCAAAAAAC TCGTTGGGTT TGGAAGTGAT GGTGCATCTG TCATGGTGGG AGAGAACAAT 
GGTGTAGCCA GGCTTCTGAA AGAAATTCAG CCATGTGTGC AAACAGTCCA CTGCTTTGCA 
CATAGACTTG AACTAGCCTA CCAAGAAGCA TTGCAGAGCA TCCAATTATA CAGTACTATG 
AATGATCTTC TCCAGAATAT ATATTATTTT TATCATGATT CACCTTCAAA TACAAAAGCT 
CTGATAGCTG CCTTTGAAGA CCTTAAAATC CGCCCTGTGA TTCCCTCTCG AGTTGGCAAC 
AACAGATGGC TCCCTCGGTT ACAGATGGCT CTACAAATCT TTCTCAAAGG ATATTCTCCA 
ATTGTCAAGC AATTAAGAAC AATGCATGAA GGCAGAAGTT CCGCTGATCA ACAGAAAGCT 
CAAGAGCTCC TACAGTTCCT TCTCCAAGCT GAGGTTGTCA GATTTGCTCA CTTCTTGCTG 
GATGTAATCA ACGTCCTCAG CATTTTGTCT CGAGTCAATC AGGACAGAAA TTCATCAATA 
GGAGATGTAT TTGCAACCCT ACAATCAACA CTAGGAACAC TCAAGATGTA TAAAAAAAGA 
CCAGGTCCAA AGGAACGTCT GACAGAGTCA GCCACACACT TTCATGGTAA CCAACTGACA 
GGAAATGGAA ATATCTCTGA AGTACGAAAC ACAGTCTTAA CAAATCTCTC AAAGCATCTT 
CATGATTGTT TCAGTGATGC CAGCCATGAT GTGGTGAGGG CGACCACTAT TGGAAGTTTC 
AAATTATGGC CAAGAAAGCT AGATCAAGAG TTTGGTGAGA AAGAGGTAAC AATTCTTACA 
AAGCATTATG AACCTGTGTT GGAAGGGGCC AATGTGAACA TTGATGAAGT GGACACAGAA 
TGGAGCATGA TGAAATTAGA GATTTATAAT CGATTTCAGA ACATCCGAAA GTTAACCTGG 
GATATTGTGA ATTCCATCTA CTCATACAAG TATCCCAATA TATTGACCCT AGTGGATCTG 
GTGCTGACAC TCCCTGCAAG TTCAGCTGAG GCGGAACGTG GGTTCAGTCA GATGAAACTA 
GCCAAGTCAC AAATGCATTT TAAAACAGAT CCTGAGAACA TGACAGACAT TTTAATAATC 
CAGTTGAATT CTCCAGACAT CAATAAGTTT GATCCCAAGA AAGCCATCCA TCTATGGAAT 
ACTTCTTGGC AGAGAAATAC ACTTCCACCA ATTGACCACA CAATAACCAA GTCAGATTGC 
TCCTCTAGCT CAGATAGTCT TTATGAAAGT GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016287099.1
CDS	440..5515
Translation	MRDWKELFFSGKPIYEIASIDSKPSRFTSEQLIELSKRNLLDDNDYEEYKNLTGEWVLPEDMADVPLPSNNNILGHVIRVVHEKVYPPKPAYIATQLPSSSIKGCMLGKTCSGKTSCLKILEEVFHIEILCMDTLVQEAIQAFLRNETVTEVETHKEDLSKEVQDIKDSNMTTIAEIIPTEEKKDENLPQQDEVIIKPPSAQDVRIRLTNRAQLGSKCEKLLKRGRSIPDVLLIQIMVNAINEISSDKGWILDGFPMTINQAKLLEEALTGFNTDQPEHEIKAHKKSTLVFDPSAPKDPPPPPPAFDFVMLLDITDTLVLNRVMGEEKRNTDTTGIPESATSEMFPEEQEKFELLRDQIQHRILGFLDNWPELEKWFSGKQDILITVNAEVDKRALATKLQELFIAELRKKEAKAKQELEEQEEERKKKEALPPEPPPPPPPEPEEEPKEAKGKGKGKGKGAKEGKGKKDTSPKGKDAAKEAKAKKSETSPKGKGRSPDKRSPEDKTSPPAPPGPPPVKAGSEDWVYVDEPLPQEIFEYLDTFWEVIESSYINTSKAIFRQIRDERHSVLYYLHNVRLNYQGYLKRPDHKQEFVSQWQEDFNNLPEDLWEDEETKAELHQRVNDLRDRLWDICDTRKEEAEQERADIIDESWLQDHLGILMNHFFSLMQVELSRFQDIKTFLQDYYRGMEGKIPTDLAKSGYTRVPLIDLDEEDTDITSKIPVIPRRQPSPEGLLNKPRSKLSLRGGRPDYCLENVQLKFERDEKTLMTIWQNTSSAINLLVNSEIQLRKLEDDKENKPTSTKEKSPQDGGKGPKGAKKKPDKKKVPSPVEAVSMVLTPEEQEALDKRNELKMKIKEEHITALQCEAQASIFRLELIKMKALSYMEELLGRAVEVYKLMDKWLGARYLAEMASVEKLTEVAHYHIETSTRIQNELILNEEDFYINGDVKVFPDPLPPVRPPPVEKEMNTFLTLEQLNTLRNEFLQMAPKAKTPWKYFKKGISSVDTRRHAEEKKRLRNLDYYLPKRNNQFLKIWLKKYPWLVYDDRSDLMFCGLCRKHGVSSGGNHVSFFYGTNNFRTEFLQAHDNSEAHTKAVLAEATGVSSLTDAAKEQILKNRNKVILGRIENLFRTCHAIAKTGRPLKDFVWMCKLDDMKGVDVGPIFRTDKSARTFIYFIAEVERICLREKLEKCKFFSLISDGGSGSFIEDTKVVYIQFAYEGRVCCQVVGVQTVDKIEPVAIKSAIEKTLEINLQLNLSSKDWSKKLVGFGSDGASVMVGENNGVARLLKEIQPCVQTVHCFAHRLELAYQEALQSIQLYSTMNDLLQNIYYFYHDSPSNTKALIAAFEDLKIRPVIPSRVGNNRWLPRLQMALQIFLKGYSPIVKQLRTMHEGRSSADQQKAQELLQFLLQAEVVRFAHFLLDVINVLSILSRVNQDRNSSIGDVFATLQSTLGTLKMYKKRPGPKERLTESATHFHGNQLTGNGNISEVRNTVLTNLSKHLHDCFSDASHDVVRATTIGSFKLWPRKLDQEFGEKEVTILTKHYEPVLEGANVNIDEVDTEWSMMKLEIYNRFQNIRKLTWDIVNSIYSYKYPNILTLVDLVLTLPASSAEAERGFSQMKLAKSQMHFKTDPENMTDILIIQLNSPDINKFDPKKAIHLWNTSWQRNTLPPIDHTITKSDCSSSSDSLYESD

Target ORF information:

RefSeq Version	XM_016431613.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016431613.1

ORF Insert Sequence:

ATGCGTGATT GGAAGGAGCT GTTTTTCAGT GGAAAGCCAA TTTATGAAAT AGCCTCCATT 
GACTCCAAGC CATCTCGTTT TACCTCAGAA CAACTAATTG AGCTATCCAA AAGGAATTTA 
CTAGATGACA ATGATTATGA GGAGTACAAG AACTTGACAG GAGAGTGGGT CCTCCCAGAA 
GATATGGCTG ATGTTCCCCT ACCTTCCAAC AATAACATAT TGGGTCATGT GATTCGAGTA 
GTTCATGAAA AAGTTTACCC TCCAAAACCA GCATATATTG CAACTCAGTT ACCTTCATCT 
TCGATTAAAG GGTGCATGCT GGGCAAGACA TGCAGTGGGA AAACCAGCTG TCTAAAGATT 
TTAGAAGAAG TTTTCCATAT TGAGATACTT TGTATGGATA CTCTGGTTCA AGAGGCTATT 
CAGGCTTTCC TTAGAAATGA AACTGTAACT GAGGTGGAAA CACATAAAGA AGATTTATCA 
AAAGAAGTTC AAGATATTAA GGATTCAAAC ATGACGACTA TTGCAGAAAT AATTCCTACT 
GAGGAGAAAA AAGATGAGAA TCTACCACAA CAAGATGAAG TGATTATTAA ACCACCATCA 
GCCCAGGATG TGAGAATAAG ACTTACTAAT CGTGCTCAAC TTGGTTCAAA ATGTGAAAAG 
TTGCTCAAGA GAGGAAGAAG TATCCCCGAT GTACTGCTAA TTCAAATCAT GGTGAATGCT 
ATTAATGAAA TATCATCAGA CAAAGGATGG ATCTTGGATG GTTTTCCAAT GACCATAAAT 
CAAGCAAAAC TTCTTGAGGA AGCCCTTACA GGGTTCAACA CAGATCAACC AGAACATGAA 
ATAAAGGCTC ACAAAAAATC AACATTGGTT TTTGATCCAT CTGCTCCCAA AGACCCCCCT 
CCTCCCCCAC CTGCATTTGA TTTTGTTATG TTGCTGGATA TAACTGACAC ATTGGTGCTG 
AATCGTGTGA TGGGAGAGGA GAAAAGAAAC ACTGACACAA CTGGTATACC AGAAAGTGCA 
ACTTCAGAAA TGTTTCCTGA AGAACAAGAA AAGTTTGAAC TTTTGAGGGA TCAGATACAA 
CACAGAATTT TAGGTTTCTT GGACAACTGG CCAGAGCTGG AAAAATGGTT TTCAGGAAAA 
CAAGACATTC TAATCACAGT CAATGCAGAA GTAGATAAAA GGGCTTTAGC CACAAAACTA 
CAAGAGCTTT TTATTGCTGA ACTGAGGAAA AAAGAAGCTA AAGCAAAACA GGAATTAGAA 
GAACAGGAAG AGGAAAGGAA AAAGAAAGAA GCCCTCCCAC CTGAACCGCC ACCTCCACCT 
CCCCCTGAAC CAGAAGAAGA GCCAAAGGAA GCAAAAGGGA AAGGAAAAGG GAAAGGTAAA 
GGTGCTAAAG AAGGAAAAGG AAAGAAAGAC ACTTCTCCCA AGGGCAAAGA TGCTGCTAAA 
GAAGCAAAAG CAAAGAAAAG TGAAACTTCT CCAAAAGGCA AAGGAAGGTC ACCAGACAAG 
AGGTCACCTG AAGATAAAAC ATCACCTCCT GCACCTCCAG GACCACCACC AGTCAAAGCA 
GGATCAGAAG ACTGGGTCTA TGTGGATGAG CCACTTCCTC AGGAAATATT TGAATATCTA 
GACACTTTTT GGGAAGTAAT AGAAAGTTCA TACATAAATA CCAGCAAAGC TATATTCAGA 
CAGATAAGAG ATGAGAGACA TAGTGTACTT TATTATCTAC ATAATGTCAG ACTAAATTAC 
CAGGGATATC TGAAACGGCC AGATCACAAG CAGGAATTTG TATCACAATG GCAGGAGGAT 
TTTAACAACC TCCCTGAAGA CTTATGGGAG GATGAGGAAA CAAAAGCTGA ACTACACCAA 
AGAGTCAATG ATTTAAGGGA CCGCCTGTGG GATATATGTG ATACTCGAAA GGAAGAGGCA 
GAACAAGAAC GTGCTGATAT CATTGATGAA TCCTGGTTAC AAGATCATCT TGGAATTCTT 
ATGAATCATT TCTTTTCTTT GATGCAGGTG GAACTTAGCC GTTTCCAGGA CATAAAGACT 
TTTCTACAAG ATTACTATAG GGGAATGGAA GGAAAAATCC CCACCGACCT TGCTAAATCT 
GGATATACTC GAGTTCCCCT TATAGATCTT GATGAAGAGG ATACCGATAT CACCTCCAAA 
ATCCCTGTAA TTCCTCGGAG ACAGCCTTCT CCTGAAGGAC TATTAAACAA GCCCAGAAGT 
AAACTATCCC TGAGAGGAGG CAGACCTGAT TACTGCTTAG AAAATGTCCA ACTCAAATTT 
GAAAGAGATG AGAAGACCCT TATGACTATA TGGCAGAATA CATCTTCAGC CATCAACCTC 
TTGGTAAATT CTGAAATACA GTTGAGGAAA CTAGAAGATG ATAAAGAAAA CAAACCAACA 
TCAACTAAAG AAAAATCTCC TCAAGATGGT GGCAAAGGTC CTAAAGGTGC TAAGAAAAAG 
CCAGATAAGA AGAAAGTTCC ATCACCAGTT GAAGCCGTTT CTATGGTTCT AACACCAGAA 
GAACAGGAGG CTCTTGATAA GAGGAATGAA TTGAAAATGA AAATAAAGGA AGAACATATT 
ACAGCTCTTC AGTGTGAAGC ACAAGCTTCA ATATTCCGAC TGGAACTGAT CAAGATGAAG 
GCCTTATCTT ACATGGAAGA GCTGCTAGGA AGAGCAGTTG AGGTTTACAA ACTGATGGAC 
AAATGGCTCG GAGCCCGATA CTTAGCTGAA ATGGCAAGTG TGGAAAAATT AACTGAAGTG 
GCTCACTACC ACATTGAAAC CTCAACAAGG ATTCAGAATG AACTTATTTT AAATGAAGAA 
GATTTCTACA TCAATGGTGA TGTGAAAGTC TTTCCAGATC CGCTTCCTCC CGTCCGGCCT 
CCACCTGTGG AGAAAGAAAT GAATACTTTT CTCACTCTTG AACAACTCAA CACCCTTCGA 
AATGAGTTTC TCCAGATGGC ACCAAAAGCC AAAACACCAT GGAAGTATTT TAAAAAAGGA 
ATCTCTTCAG TTGATACTAG AAGACATGCT GAGGAAAAGA AAAGGCTAAG AAATCTTGAC 
TATTACCTAC CTAAGAGGAA CAACCAATTT TTGAAAATTT GGCTAAAAAA ATACCCTTGG 
CTCGTGTATG ATGACAGGTC AGACTTAATG TTCTGTGGCT TGTGTCGCAA ACATGGAGTG 
AGCTCAGGAG GAAACCATGT TAGCTTTTTT TATGGCACCA ACAACTTTAG GACTGAATTT 
CTCCAAGCAC ATGACAATAG TGAGGCACAC ACGAAGGCTG TTCTAGCAGA AGCAACAGGG 
GTTTCTTCCC TGACTGATGC TGCCAAGGAG CAGATTCTAA AGAACAGGAA CAAAGTAATC 
TTGGGAAGAA TAGAAAACCT CTTTAGAACA TGTCATGCTA TTGCCAAGAC TGGACGTCCC 
CTCAAAGACT TTGTCTGGAT GTGTAAACTG GATGATATGA AGGGTGTAGA CGTTGGCCCA 
ATATTCAGGA CTGACAAGTC AGCCAGAACT TTCATATACT TTATTGCTGA AGTAGAACGC 
ATATGTCTTC GAGAGAAATT AGAAAAATGT AAGTTCTTCT CACTCATCAG TGATGGTGGC 
TCAGGCAGCT TCATAGAAGA CACCAAAGTT GTTTACATCC AGTTTGCATA TGAGGGCAGG 
GTCTGCTGTC AGGTTGTTGG AGTACAGACT GTGGACAAAA TTGAACCGGT GGCAATAAAA 
AGTGCCATTG AAAAAACACT TGAGATAAAT CTTCAACTAA ATCTGTCTAG CAAAGACTGG 
TCCAAAAAAC TCGTTGGGTT TGGAAGTGAT GGTGCATCTG TCATGGTGGG AGAGAACAAT 
GGTGTAGCCA GGCTTCTGAA AGAAATTCAG CCATGTGTGC AAACAGTCCA CTGCTTTGCA 
CATAGACTTG AACTAGCCTA CCAAGAAGCA TTGCAGAGCA TCCAATTATA CAGTACTATG 
AATGATCTTC TCCAGAATAT ATATTATTTT TATCATGATT CACCTTCAAA TACAAAAGCT 
CTGATAGCTG CCTTTGAAGA CCTTAAAATC CGCCCTGTGA TTCCCTCTCG AGTTGGCAAC 
AACAGATGGC TCCCTCGGTT ACAGATGGCT CTACAAATCT TTCTCAAAGG ATATTCTCCA 
ATTGTCAAGC AATTAAGAAC AATGCATGAA GGCAGAAGTT CCGCTGATCA ACAGAAAGCT 
CAAGAGCTCC TACAGTTCCT TCTCCAAGCT GAGGTTGTCA GATTTGCTCA CTTCTTGCTG 
GATGTAATCA ACGTCCTCAG CATTTTGTCT CGAGTCAATC AGGACAGAAA TTCATCAATA 
GGAGATGTAT TTGCAACCCT ACAATCAACA CTAGGAACAC TCAAGATGTA TAAAAAAAGA 
CCAGGTCCAA AGGAACGTCT GACAGAGTCA GCCACACACT TTCATGGTAA CCAACTGACA 
GGAAATGGAA ATATCTCTGA AGTACGAAAC ACAGTCTTAA CAAATCTCTC AAAGCATCTT 
CATGATTGTT TCAGTGATGC CAGCCATGAT GTGGTGAGGG CGACCACTAT TGGAAGTTTC 
AAATTATGGC CAAGAAAGCT AGATCAAGAG TTTGGTGAGA AAGAGGTAAC AATTCTTACA 
AAGCATTATG AACCTGTGTT GGAAGGGGCC AATGTGAACA TTGATGAAGT GGACACAGAA 
TGGAGCATGA TGAAATTAGA GATTTATAAT CGATTTCAGA ACATCCGAAA GTTAACCTGG 
GATATTGTGA ATTCCATCTA CTCATACAAG TATCCCAATA TATTGACCCT AGTGGATCTG 
GTGCTGACAC TCCCTGCAAG TTCAGCTGAG GCGGAACGTG GGTTCAGTCA GATGAAACTA 
GCCAAGTCAC AAATGCATTT TAAAACAGAT CCTGAGAACA TGACAGACAT TTTAATAATC 
CAGTTGAATT CTCCAGACAT CAATAAGTTT GATCCCAAGA AAGCCATCCA TCTATGGAAT 
ACTTCTTGGC AGAGAAATAC ACTTCCACCA ATTGACCACA CAATAACCAA GTCAGATTGC 
TCCTCTAGCT CAGATAGTCT TTATGAAAGT GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_007487458.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007487520.2 Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X9, mRNA.

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CloneID	OMc41049
Clone ID Related Accession (Same CDS sequence)	XM_007487458.2
Accession Version	XM_007487458.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	sperm flagellar protein 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008803.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007487458.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACA TCCTGTGCAG GTGGCTAAAC CAGGAGGTGA AGCTGTCCCG GACAGTGAGT 
TCAAAGGGGA TTGAAAAAGA TTTTTCTAAT GGCTTCCTAC TGGGTGAACT TCTGCACAAA 
TATGAACTTC AGGATGACTT TCCTCTGTTT TCAGAAGGCA GGGTTTCCAA TGCAAAACTT 
AATAATTTTT CTCGCTTAGA ACCAACATTT CAACTCCTGG GTGTTCAGTT CGATCAAAAT 
GTGGCCCAGA ATATTATGAC AGAAAAGCAT GGCTCTGTAA CCATACTTCT TTATCAGTTG 
TACATTGCGC TTCAAAAAAA GAGAAAAAGT GGACTCACGG GAGTAGCAAT GCAAACAATG 
AGACCTTTAG CACCTGCAAA GCTACAAACT ATGAGAAGTG AGATTTATCG AGAGAGACTT 
AAGAACCTGG TGCCTCGGCA AACTGATTTG AATCTAAGTC GGGTTTCAGA AAAGTTTGAA 
GCAAAGTATA AAAAAATGGA AGAAAAAATT GCTCGTGTTC ATTTTCAAAA TATCCGAAAA 
TTTGAAAAGT TCCAGGAAGA ACAAAGAATT CAAGATCTTG AAAAGCAGCG CCTGGGAAGA 
AGACGACAAC ATGAAATGAT GGCTAGAATC CAAGCAGCCA TTTTACAGAT TCCCAAAACA 
GTACCCAATC GTACTCTGAA ATCTATTGAG AGCCACAAAA TGATGATGAA GAAAAAAGAG 
GAAGAAGATG TGCTGAATGA AATTCAGAAG TTTGAAATGC TAATAAAAAA GGAGCTTGAG 
GCCAAAGGCA GTGGATCAAA AGTTCCTTCG GCAGCCCAGA CAACTGCTGA CTTGTTAAGC 
ACGTATTCAG ATGAGGAATA CATTAAAAAA ATTCAAAAAC GTCTAGAGGA AGATACCTTA 
GCCCGGGAAC AAAGGGAGAA AAGACGCAGA AAATTGTTAA TGGATCAATT GATAGCCCAT 
GAAGCTCAAG AGGAGGCTTA CCGAGAAGAA CAACTCATTA ACCGACTAAT GAGACAATCT 
CAGCAGGAAC GTCGGATTGC TGTGCAACTC ATGCATGTTC GACACGAAAA GGAAGTTTTA 
TGGCAAAACA GGGTTTTCAG AGAAAAACAA TATGAAGAAA GACGGCTGAA AGATTTCCAG 
GATGCTCTTG ACAGAGAAGC AGCTCTTGCA AGACAAGCCA AGCTGGAGTA TGAAGAACAA 
GCCCAAAAGG AAATAGAAAT TCATGAAAGA ATAGCAGCAG AAAGATCTCA AGCTCGTTAT 
AAAAAGCATT ATTCAATGTG CACAGAAATC TTGGACAATA TCATTGATTT ATCAACTAAA 
ATAGCAGAAT ATCGAGAATT AACACATAAT CTTATTCCAG TTAAAATTAT GCGTGATTGG 
AAGGAGCTGT TTTTCAGTGG AAAGCCAATT TATGAAATAG CCTCCATTGA CTCCAAGCCA 
TCTCGTTTTA CCTCAGAACA ACTAATTGAG CTATCCAAAA GGAATTTACT AGATGACAAT 
GATTATGAGG AGTACAAGAA CTTGACAGGA GAGTGGGTCC TCCCAGAAGA TATGGCTGAT 
GTTCCCCTAC CTTCCAACAA TAACATATTG GGTCATGTGA TTCGAGTAGT TCATGAAAAA 
GTTTACCCTC CAAAACCAGC ATATATTGCA ACTCAGTTAC CTTCATCTTC GATTAAAGGG 
TGCATGCTGG GCAAGACATG CAGTGGGAAA ACCAGCTGTC TAAAGATTTT AGAAGAAGTT 
TTCCATATTG AGATACTTTG TATGGATACT CTGGTTCAAG AGGCTATTCA GGCTTTCCTT 
AGAAATGAAA CTGTAACTGA GGTGGAAACA CATAAAGAAG ATTTATCAAA AGAAGTTCAA 
GATATTAAGG ATTCAAACAT GACGACTATT GCAGAAATAA TTCCTACTGA GGAGAAAAAA 
GATGAGAATC TACCACAACA AGATGAAGTG ATTATTAAAC CACCATCAGC CCAGGATGTG 
AGAATAAGAC TTACTAATCG TGCTCAACTT GGTTCAAAAT GTGAAAAGTT GCTCAAGAGA 
GGAAGAAGTA TCCCCGATGT ACTGCTAATT CAAATCATGG TGAATGCTAT TAATGAAATA 
TCATCAGACA AAGGATGGAT CTTGGATGGT TTTCCAATGA CCATAAATCA AGCAAAACTT 
CTTGAGGAAG CCCTTACAGG GTTCAACACA GATCAACCAG AACATGAAAT AAAGGCTCAC 
AAAAAATCAA CATTGGTTTT TGATCCATCT GCTCCCAAAG ACCCCCCTCC TCCCCCACCT 
GCATTTGATT TTGTTATGTT GCTGGATATA ACTGACACAT TGGTGCTGAA TCGTGTGATG 
GGAGAGGAGA AAAGAAACAC TGACACAACT GGTATACCAG AAAGTGCAAC TTCAGAAATG 
TTTCCTGAAG AACAAGAAAA GTTTGAACTT TTGAGGGATC AGATACAACA CAGAATTTTA 
GGTTTCTTGG ACAACTGGCC AGAGCTGGAA AAATGGTTTT CAGGAAAACA AGACATTCTA 
ATCACAGTCA ATGCAGAAGT AGATAAAAGG GCTTTAGCCA CAAAACTACA AGAGCTTTTT 
ATTGCTGAAC TGAGGAAAAA AGAAGCTAAA GCAAAACAGG AATTAGAAGA ACAGGAAGAG 
GAAAGGAAAA AGAAAGAAGC CCTCCCACCT GAACCGCCAC CTCCACCTCC CCCTGAACCA 
GAAGAAGAGC CAAAGGAAGC AAAAGGGAAA GGAAAAGGGA AAGGTAAAGG TGCTAAAGAA 
GGAAAAGGAA AGAAAGACAC TTCTCCCAAG GGCAAAGATG CTGCTAAAGA AGCAAAAGCA 
AAGAAAAGTG AAACTTCTCC AAAAGGCAAA GGAAGGTCAC CAGACAAGAG GTCACCTGAA 
GATAAAACAT CACCTCCTGC ACCTCCAGGA CCACCACCAG TCAAAGCAGG ATCAGAAGAC 
TGGGTCTATG TGGATGAGCC ACTTCCTCAG GAAATATTTG AATATCTAGA CACTTTTTGG 
GAAGTAATAG AAAGTTCATA CATAAATACC AGCAAAGCTA TATTCAGACA GATAAGAGAT 
GAGAGACATA GTGTACTTTA TTATCTACAT AATGTCAGAC TAAATTACCA GGGATATCTG 
AAACGGCCAG ATCACAAGCA GGAATTTGTA TCACAATGGC AGGAGGATTT TAACAACCTC 
CCTGAAGACT TATGGGAGGA TGAGGAAACA AAAGCTGAAC TACACCAAAG AGTCAATGAT 
TTAAGGGACC GCCTGTGGGA TATATGTGAT ACTCGAAAGG AAGAGGCAGA ACAAGAACGT 
GCTGATATCA TTGATGAATC CTGGTTACAA GATCATCTTG GAATTCTTAT GAATCATTTC 
TTTTCTTTGA TGCAGGTGGA ACTTAGCCGT TTCCAGGACA TAAAGACTTT TCTACAAGAT 
TACTATAGGG GAATGGAAGG AAAAATCCCC ACCGACCTTG CTAAATCTGG ATATACTCGA 
GTTCCCCTTA TAGATCTTGA TGAAGAGGAT ACCGATATCA CCTCCAAAAT CCCTGTAATT 
CCTCGGAGAC AGCCTTCTCC TGAAGGACTA TTAAACAAGC CCAGAAGTAA ACTATCCCTG 
AGAGGAGGCA GACCTGATTA CTGCTTAGAA AATGTCCAAC TCAAATTTGA AAGAGATGAG 
AAGACCCTTA TGACTATATG GCAGAATACA TCTTCAGCCA TCAACCTCTT GGTAAATTCT 
GAAATACAGT TGAGGAAACT AGAAGATGAT AAAGAAAACA AACCAACATC AACTAAAGAA 
AAATCTCCTC AAGATGGTGG CAAAGGTCCT AAAGGTGCTA AGAAAAAGCC AGATAAGAAG 
AAAGTTCCAT CACCAGTTGA AGCCGTTTCT ATGGTTCTAA CACCAGAAGA ACAGGAGGCT 
CTTGATAAGA GGAATGAATT GAAAATGAAA ATAAAGGAAG AACATATTAC AGCTCTTCAG 
TGTGAAGCAC AAGCTTCAAT ATTCCGACTG GAACTGATCA AGATGAAGGC CTTATCTTAC 
ATGGAAGAGC TGCTAGGAAG AGCAGTTGAG GTTTACAAAC TGATGGACAA ATGGCTCGGA 
GCCCGATACT TAGCTGAAAT GGCAAGTGTG GAAAAATTAA CTGAAGTGGC TCACTACCAC 
ATTGAAACCT CAACAAGGAT TCAGAATGAA CTTATTTTAA ATGAAGAAGA TTTCTACATC 
AATGGTGATG TGAAAGTCTT TCCAGATCCG CTTCCTCCCG TCCGGCCTCC ACCTGTGGAG 
AAAGAAATGA ATACTTTTCT CACTCTTGAA CAACTCAACA CCCTTCGAAA TGAGTTTCTC 
CAGATGGCAC CAAAAGGTTA TTTGGGAAGT AAACATTTCA CTGATATTTT GTCTGACTTG 
GTGACTTTGA ACCTTGGAAC AAACCACATT CCCAAGGCTT GGCTGCACCT CATTCTTTCT 
GAATTACAAG AGCTAACATC TTTATTAGTA GTGAATTCAG AGTTTGTTGA CTGGCGCAGA 
TTTTTATTAG TAGTTTCACA GCCTTGGCCA TTGCCATCTG AGGAAGAGCT TCTTGAGACA 
TTGAACAGAT TTAAAGCAAT TGATGAAGAT GAAAAAGGCA CAGTAACCTG GGAGCAGTAT 
GAGCAGGTGA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007487520.2
CDS	80..4714
Translation	MSDILCRWLNQEVKLSRTVSSKGIEKDFSNGFLLGELLHKYELQDDFPLFSEGRVSNAKLNNFSRLEPTFQLLGVQFDQNVAQNIMTEKHGSVTILLYQLYIALQKKRKSGLTGVAMQTMRPLAPAKLQTMRSEIYRERLKNLVPRQTDLNLSRVSEKFEAKYKKMEEKIARVHFQNIRKFEKFQEEQRIQDLEKQRLGRRRQHEMMARIQAAILQIPKTVPNRTLKSIESHKMMMKKKEEEDVLNEIQKFEMLIKKELEAKGSGSKVPSAAQTTADLLSTYSDEEYIKKIQKRLEEDTLAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRVFREKQYEERRLKDFQDALDREAALARQAKLEYEEQAQKEIEIHERIAAERSQARYKKHYSMCTEILDNIIDLSTKIAEYRELTHNLIPVKIMRDWKELFFSGKPIYEIASIDSKPSRFTSEQLIELSKRNLLDDNDYEEYKNLTGEWVLPEDMADVPLPSNNNILGHVIRVVHEKVYPPKPAYIATQLPSSSIKGCMLGKTCSGKTSCLKILEEVFHIEILCMDTLVQEAIQAFLRNETVTEVETHKEDLSKEVQDIKDSNMTTIAEIIPTEEKKDENLPQQDEVIIKPPSAQDVRIRLTNRAQLGSKCEKLLKRGRSIPDVLLIQIMVNAINEISSDKGWILDGFPMTINQAKLLEEALTGFNTDQPEHEIKAHKKSTLVFDPSAPKDPPPPPPAFDFVMLLDITDTLVLNRVMGEEKRNTDTTGIPESATSEMFPEEQEKFELLRDQIQHRILGFLDNWPELEKWFSGKQDILITVNAEVDKRALATKLQELFIAELRKKEAKAKQELEEQEEERKKKEALPPEPPPPPPPEPEEEPKEAKGKGKGKGKGAKEGKGKKDTSPKGKDAAKEAKAKKSETSPKGKGRSPDKRSPEDKTSPPAPPGPPPVKAGSEDWVYVDEPLPQEIFEYLDTFWEVIESSYINTSKAIFRQIRDERHSVLYYLHNVRLNYQGYLKRPDHKQEFVSQWQEDFNNLPEDLWEDEETKAELHQRVNDLRDRLWDICDTRKEEAEQERADIIDESWLQDHLGILMNHFFSLMQVELSRFQDIKTFLQDYYRGMEGKIPTDLAKSGYTRVPLIDLDEEDTDITSKIPVIPRRQPSPEGLLNKPRSKLSLRGGRPDYCLENVQLKFERDEKTLMTIWQNTSSAINLLVNSEIQLRKLEDDKENKPTSTKEKSPQDGGKGPKGAKKKPDKKKVPSPVEAVSMVLTPEEQEALDKRNELKMKIKEEHITALQCEAQASIFRLELIKMKALSYMEELLGRAVEVYKLMDKWLGARYLAEMASVEKLTEVAHYHIETSTRIQNELILNEEDFYINGDVKVFPDPLPPVRPPPVEKEMNTFLTLEQLNTLRNEFLQMAPKGYLGSKHFTDILSDLVTLNLGTNHIPKAWLHLILSELQELTSLLVVNSEFVDWRRFLLVVSQPWPLPSEEELLETLNRFKAIDEDEKGTVTWEQYEQVI

Target ORF information:

RefSeq Version	XM_007487458.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007487458.2

ORF Insert Sequence:

ATGTCGGACA TCCTGTGCAG GTGGCTAAAC CAGGAGGTGA AGCTGTCCCG GACAGTGAGT 
TCAAAGGGGA TTGAAAAAGA TTTTTCTAAT GGCTTCCTAC TGGGTGAACT TCTGCACAAA 
TATGAACTTC AGGATGACTT TCCTCTGTTT TCAGAAGGCA GGGTTTCCAA TGCAAAACTT 
AATAATTTTT CTCGCTTAGA ACCAACATTT CAACTCCTGG GTGTTCAGTT CGATCAAAAT 
GTGGCCCAGA ATATTATGAC AGAAAAGCAT GGCTCTGTAA CCATACTTCT TTATCAGTTG 
TACATTGCGC TTCAAAAAAA GAGAAAAAGT GGACTCACGG GAGTAGCAAT GCAAACAATG 
AGACCTTTAG CACCTGCAAA GCTACAAACT ATGAGAAGTG AGATTTATCG AGAGAGACTT 
AAGAACCTGG TGCCTCGGCA AACTGATTTG AATCTAAGTC GGGTTTCAGA AAAGTTTGAA 
GCAAAGTATA AAAAAATGGA AGAAAAAATT GCTCGTGTTC ATTTTCAAAA TATCCGAAAA 
TTTGAAAAGT TCCAGGAAGA ACAAAGAATT CAAGATCTTG AAAAGCAGCG CCTGGGAAGA 
AGACGACAAC ATGAAATGAT GGCTAGAATC CAAGCAGCCA TTTTACAGAT TCCCAAAACA 
GTACCCAATC GTACTCTGAA ATCTATTGAG AGCCACAAAA TGATGATGAA GAAAAAAGAG 
GAAGAAGATG TGCTGAATGA AATTCAGAAG TTTGAAATGC TAATAAAAAA GGAGCTTGAG 
GCCAAAGGCA GTGGATCAAA AGTTCCTTCG GCAGCCCAGA CAACTGCTGA CTTGTTAAGC 
ACGTATTCAG ATGAGGAATA CATTAAAAAA ATTCAAAAAC GTCTAGAGGA AGATACCTTA 
GCCCGGGAAC AAAGGGAGAA AAGACGCAGA AAATTGTTAA TGGATCAATT GATAGCCCAT 
GAAGCTCAAG AGGAGGCTTA CCGAGAAGAA CAACTCATTA ACCGACTAAT GAGACAATCT 
CAGCAGGAAC GTCGGATTGC TGTGCAACTC ATGCATGTTC GACACGAAAA GGAAGTTTTA 
TGGCAAAACA GGGTTTTCAG AGAAAAACAA TATGAAGAAA GACGGCTGAA AGATTTCCAG 
GATGCTCTTG ACAGAGAAGC AGCTCTTGCA AGACAAGCCA AGCTGGAGTA TGAAGAACAA 
GCCCAAAAGG AAATAGAAAT TCATGAAAGA ATAGCAGCAG AAAGATCTCA AGCTCGTTAT 
AAAAAGCATT ATTCAATGTG CACAGAAATC TTGGACAATA TCATTGATTT ATCAACTAAA 
ATAGCAGAAT ATCGAGAATT AACACATAAT CTTATTCCAG TTAAAATTAT GCGTGATTGG 
AAGGAGCTGT TTTTCAGTGG AAAGCCAATT TATGAAATAG CCTCCATTGA CTCCAAGCCA 
TCTCGTTTTA CCTCAGAACA ACTAATTGAG CTATCCAAAA GGAATTTACT AGATGACAAT 
GATTATGAGG AGTACAAGAA CTTGACAGGA GAGTGGGTCC TCCCAGAAGA TATGGCTGAT 
GTTCCCCTAC CTTCCAACAA TAACATATTG GGTCATGTGA TTCGAGTAGT TCATGAAAAA 
GTTTACCCTC CAAAACCAGC ATATATTGCA ACTCAGTTAC CTTCATCTTC GATTAAAGGG 
TGCATGCTGG GCAAGACATG CAGTGGGAAA ACCAGCTGTC TAAAGATTTT AGAAGAAGTT 
TTCCATATTG AGATACTTTG TATGGATACT CTGGTTCAAG AGGCTATTCA GGCTTTCCTT 
AGAAATGAAA CTGTAACTGA GGTGGAAACA CATAAAGAAG ATTTATCAAA AGAAGTTCAA 
GATATTAAGG ATTCAAACAT GACGACTATT GCAGAAATAA TTCCTACTGA GGAGAAAAAA 
GATGAGAATC TACCACAACA AGATGAAGTG ATTATTAAAC CACCATCAGC CCAGGATGTG 
AGAATAAGAC TTACTAATCG TGCTCAACTT GGTTCAAAAT GTGAAAAGTT GCTCAAGAGA 
GGAAGAAGTA TCCCCGATGT ACTGCTAATT CAAATCATGG TGAATGCTAT TAATGAAATA 
TCATCAGACA AAGGATGGAT CTTGGATGGT TTTCCAATGA CCATAAATCA AGCAAAACTT 
CTTGAGGAAG CCCTTACAGG GTTCAACACA GATCAACCAG AACATGAAAT AAAGGCTCAC 
AAAAAATCAA CATTGGTTTT TGATCCATCT GCTCCCAAAG ACCCCCCTCC TCCCCCACCT 
GCATTTGATT TTGTTATGTT GCTGGATATA ACTGACACAT TGGTGCTGAA TCGTGTGATG 
GGAGAGGAGA AAAGAAACAC TGACACAACT GGTATACCAG AAAGTGCAAC TTCAGAAATG 
TTTCCTGAAG AACAAGAAAA GTTTGAACTT TTGAGGGATC AGATACAACA CAGAATTTTA 
GGTTTCTTGG ACAACTGGCC AGAGCTGGAA AAATGGTTTT CAGGAAAACA AGACATTCTA 
ATCACAGTCA ATGCAGAAGT AGATAAAAGG GCTTTAGCCA CAAAACTACA AGAGCTTTTT 
ATTGCTGAAC TGAGGAAAAA AGAAGCTAAA GCAAAACAGG AATTAGAAGA ACAGGAAGAG 
GAAAGGAAAA AGAAAGAAGC CCTCCCACCT GAACCGCCAC CTCCACCTCC CCCTGAACCA 
GAAGAAGAGC CAAAGGAAGC AAAAGGGAAA GGAAAAGGGA AAGGTAAAGG TGCTAAAGAA 
GGAAAAGGAA AGAAAGACAC TTCTCCCAAG GGCAAAGATG CTGCTAAAGA AGCAAAAGCA 
AAGAAAAGTG AAACTTCTCC AAAAGGCAAA GGAAGGTCAC CAGACAAGAG GTCACCTGAA 
GATAAAACAT CACCTCCTGC ACCTCCAGGA CCACCACCAG TCAAAGCAGG ATCAGAAGAC 
TGGGTCTATG TGGATGAGCC ACTTCCTCAG GAAATATTTG AATATCTAGA CACTTTTTGG 
GAAGTAATAG AAAGTTCATA CATAAATACC AGCAAAGCTA TATTCAGACA GATAAGAGAT 
GAGAGACATA GTGTACTTTA TTATCTACAT AATGTCAGAC TAAATTACCA GGGATATCTG 
AAACGGCCAG ATCACAAGCA GGAATTTGTA TCACAATGGC AGGAGGATTT TAACAACCTC 
CCTGAAGACT TATGGGAGGA TGAGGAAACA AAAGCTGAAC TACACCAAAG AGTCAATGAT 
TTAAGGGACC GCCTGTGGGA TATATGTGAT ACTCGAAAGG AAGAGGCAGA ACAAGAACGT 
GCTGATATCA TTGATGAATC CTGGTTACAA GATCATCTTG GAATTCTTAT GAATCATTTC 
TTTTCTTTGA TGCAGGTGGA ACTTAGCCGT TTCCAGGACA TAAAGACTTT TCTACAAGAT 
TACTATAGGG GAATGGAAGG AAAAATCCCC ACCGACCTTG CTAAATCTGG ATATACTCGA 
GTTCCCCTTA TAGATCTTGA TGAAGAGGAT ACCGATATCA CCTCCAAAAT CCCTGTAATT 
CCTCGGAGAC AGCCTTCTCC TGAAGGACTA TTAAACAAGC CCAGAAGTAA ACTATCCCTG 
AGAGGAGGCA GACCTGATTA CTGCTTAGAA AATGTCCAAC TCAAATTTGA AAGAGATGAG 
AAGACCCTTA TGACTATATG GCAGAATACA TCTTCAGCCA TCAACCTCTT GGTAAATTCT 
GAAATACAGT TGAGGAAACT AGAAGATGAT AAAGAAAACA AACCAACATC AACTAAAGAA 
AAATCTCCTC AAGATGGTGG CAAAGGTCCT AAAGGTGCTA AGAAAAAGCC AGATAAGAAG 
AAAGTTCCAT CACCAGTTGA AGCCGTTTCT ATGGTTCTAA CACCAGAAGA ACAGGAGGCT 
CTTGATAAGA GGAATGAATT GAAAATGAAA ATAAAGGAAG AACATATTAC AGCTCTTCAG 
TGTGAAGCAC AAGCTTCAAT ATTCCGACTG GAACTGATCA AGATGAAGGC CTTATCTTAC 
ATGGAAGAGC TGCTAGGAAG AGCAGTTGAG GTTTACAAAC TGATGGACAA ATGGCTCGGA 
GCCCGATACT TAGCTGAAAT GGCAAGTGTG GAAAAATTAA CTGAAGTGGC TCACTACCAC 
ATTGAAACCT CAACAAGGAT TCAGAATGAA CTTATTTTAA ATGAAGAAGA TTTCTACATC 
AATGGTGATG TGAAAGTCTT TCCAGATCCG CTTCCTCCCG TCCGGCCTCC ACCTGTGGAG 
AAAGAAATGA ATACTTTTCT CACTCTTGAA CAACTCAACA CCCTTCGAAA TGAGTTTCTC 
CAGATGGCAC CAAAAGGTTA TTTGGGAAGT AAACATTTCA CTGATATTTT GTCTGACTTG 
GTGACTTTGA ACCTTGGAAC AAACCACATT CCCAAGGCTT GGCTGCACCT CATTCTTTCT 
GAATTACAAG AGCTAACATC TTTATTAGTA GTGAATTCAG AGTTTGTTGA CTGGCGCAGA 
TTTTTATTAG TAGTTTCACA GCCTTGGCCA TTGCCATCTG AGGAAGAGCT TCTTGAGACA 
TTGAACAGAT TTAAAGCAAT TGATGAAGAT GAAAAAGGCA CAGTAACCTG GGAGCAGTAT 
GAGCAGGTGA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_016431606.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016287092.1 Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.

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CloneID	OMc41041
Clone ID Related Accession (Same CDS sequence)	XM_016431606.1
Accession Version	XM_016431606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	sperm flagellar protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008803.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACA TCCTGTGCAG GTGGCTAAAC CAGGAGGTGA AGCTGTCCCG GACAGTGAGT 
TCAAAGGGGA TTGAAAAAGA TTTTTCTAAT GGCTTCCTAC TGGGTGAACT TCTGCACAAA 
TATGAACTTC AGGATGACTT TCCTCTGTTT TCAGAAGGCA GGGTTTCCAA TGCAAAACTT 
AATAATTTTT CTCGCTTAGA ACCAACATTT CAACTCCTGG GTGTTCAGTT CGATCAAAAT 
GTGGCCCAGA ATATTATGAC AGAAAAGCAT GGCTCTGTAA CCATACTTCT TTATCAGTTG 
TACATTGCGC TTCAAAAAAA GAGAAAAAGT GGACTCACGG GAGTAGCAAT GCAAACAATG 
AGACCTTTAG CACCTGCAAA GCTACAAACT ATGAGAAGTG AGATTTATCG AGAGAGACTT 
AAGAACCTGG TGCCTCGGCA AACTGATTTG AATCTAAGTC GGGTTTCAGA AAAGTTTGAA 
GCAAAGTATA AAAAAATGGA AGAAAAAATT GCTCGTGTTC ATTTTCAAAA TATCCGAAAA 
TTTGAAAAGT TCCAGGAAGA ACAAAGAATT CAAGATCTTG AAAAGCAGCG CCTGGGAAGA 
AGACGACAAC ATGAAATGAT GGCTAGAATC CAAGCAGCCA TTTTACAGAT TCCCAAAACA 
GTACCCAATC GTACTCTGAA ATCTATTGAG AGCCACAAAA TGATGATGAA GAAAAAAGAG 
GAAGAAGATG TGCTGAATGA AATTCAGAAG TTTGAAATGC TAATAAAAAA GGAGCTTGAG 
GCCAAAGGCA GTGGATCAAA AGTTCCTTCG GCAGCCCAGA CAACTGCTGA CTTGTTAAGC 
ACGTATTCAG ATGAGGAATA CATTAAAAAA ATTCAAAAAC GTCTAGAGGA AGATACCTTA 
GCCCGGGAAC AAAGGGAGAA AAGACGCAGA AAATTGTTAA TGGATCAATT GATAGCCCAT 
GAAGCTCAAG AGGAGGCTTA CCGAGAAGAA CAACTCATTA ACCGACTAAT GAGACAATCT 
CAGCAGGAAC GTCGGATTGC TGTGCAACTC ATGCATGTTC GACACGAAAA GGAAGTTTTA 
TGGCAAAACA GGGTTTTCAG AGAAAAACAA TATGAAGAAA GACGGCTGAA AGATTTCCAG 
GATGCTCTTG ACAGAGAAGC AGCTCTTGCA AGACAAGCCA AGCTGGAGTA TGAAGAACAA 
GCCCAAAAGG AAATAGAAAT TCATGAAAGA ATAGCAGCAG AAAGATCTCA AGCTCGTTAT 
AAAAAGCATT ATTCAATGTG CACAGAAATC TTGGACAATA TCATTGATTT ATCAACTAAA 
ATAGCAGAAT ATCGAGAATT AACACATAAT CTTATTCCAG TTAAAATTAT GCGTGATTGG 
AAGGAGCTGT TTTTCAGTGG AAAGCCAATT TATGAAATAG CCTCCATTGA CTCCAAGCCA 
TCTCGTTTTA CCTCAGAACA ACTAATTGAG CTATCCAAAA GGAATTTACT AGATGACAAT 
GATTATGAGG AGTACAAGAA CTTGACAGGA GAGTGGGTCC TCCCAGAAGA TATGGCTGAT 
GTTCCCCTAC CTTCCAACAA TAACATATTG GGTCATGTGA TTCGAGTAGT TCATGAAAAA 
GTTTACCCTC CAAAACCAGC ATATATTGCA ACTCAGTTAC CTTCATCTTC GATTAAAGGG 
TGCATGCTGG GCAAGACATG CAGTGGGAAA ACCAGCTGTC TAAAGATTTT AGAAGAAGTT 
TTCCATATTG AGATACTTTG TATGGATACT CTGGTTCAAG AGGCTATTCA GGCTTTCCTT 
AGAAATGAAA CTGTAACTGA GGTGGAAACA CATAAAGAAG ATTTATCAAA AGAAGTTCAA 
GATATTAAGG ATTCAAACAT GACGACTATT GCAGAAATAA TTCCTACTGA GGAGAAAAAA 
GATGAGAATC TACCACAACA AGATGAAGTG ATTATTAAAC CACCATCAGC CCAGGATGTG 
AGAATAAGAC TTACTAATCG TGCTCAACTT GGTTCAAAAT GTGAAAAGTT GCTCAAGAGA 
GGAAGAAGTA TCCCCGATGT ACTGCTAATT CAAATCATGG TGAATGCTAT TAATGAAATA 
TCATCAGACA AAGGATGGAT CTTGGATGGT TTTCCAATGA CCATAAATCA AGCAAAACTT 
CTTGAGGAAG CCCTTACAGG GTTCAACACA GATCAACCAG AACATGAAAT AAAGGCTCAC 
AAAAAATCAA CATTGGTTTT TGATCCATCT GCTCCCAAAG ACCCCCCTCC TCCCCCACCT 
GCATTTGATT TTGTTATGTT GCTGGATATA ACTGACACAT TGGTGCTGAA TCGTGTGATG 
GGAGAGGAGA AAAGAAACAC TGACACAACT GGTATACCAG AAAGTGCAAC TTCAGAAATG 
TTTCCTGAAG AACAAGAAAA GTTTGAACTT TTGAGGGATC AGATACAACA CAGAATTTTA 
GGTTTCTTGG ACAACTGGCC AGAGCTGGAA AAATGGTTTT CAGGAAAACA AGACATTCTA 
ATCACAGTCA ATGCAGAAGT AGATAAAAGG GCTTTAGCCA CAAAACTACA AGAGCTTTTT 
ATTGCTGAAC TGAGGAAAAA AGAAGCTAAA GCAAAACAGG AATTAGAAGA ACAGGAAGAG 
GAAAGGAAAA AGAAAGAAGC CCTCCCACCT GAACCGCCAC CTCCACCTCC CCCTGAACCA 
GAAGAAGAGC CAAAGGAAGC AAAAGGGAAA GGAAAAGGGA AAGGTAAAGG TGCTAAAGAA 
GGAAAAGGAA AGAAAGACAC TTCTCCCAAG GGCAAAGATG CTGCTAAAGA AGCAAAAGCA 
AAGAAAAGTG AAACTTCTCC AAAAGGCAAA GGAAGGTCAC CAGACAAGAG GTCACCTGAA 
GATAAAACAT CACCTCCTGC ACCTCCAGGA CCACCACCAG TCAAAGCAGG ATCAGAAGAC 
TGGGTCTATG TGGATGAGCC ACTTCCTCAG GAAATATTTG AATATCTAGA CACTTTTTGG 
GAAGTAATAG AAAGTTCATA CATAAATACC AGCAAAGCTA TATTCAGACA GATAAGAGAT 
GAGAGACATA GTGTACTTTA TTATCTACAT AATGTCAGAC TAAATTACCA GGGATATCTG 
AAACGGCCAG ATCACAAGCA GGAATTTGTA TCACAATGGC AGGAGGATTT TAACAACCTC 
CCTGAAGACT TATGGGAGGA TGAGGAAACA AAAGCTGAAC TACACCAAAG AGTCAATGAT 
TTAAGGGACC GCCTGTGGGA TATATGTGAT ACTCGAAAGG AAGAGGCAGA ACAAGAACGT 
GCTGATATCA TTGATGAATC CTGGTTACAA GATCATCTTG GAATTCTTAT GAATCATTTC 
TTTTCTTTGA TGCAGGTGGA ACTTAGCCGT TTCCAGGACA TAAAGACTTT TCTACAAGAT 
TACTATAGGG GAATGGAAGG AAAAATCCCC ACCGACCTTG CTAAATCTGG ATATACTCGA 
GTTCCCCTTA TAGATCTTGA TGAAGAGGAT ACCGATATCA CCTCCAAAAT CCCTGTAATT 
CCTCGGAGAC AGCCTTCTCC TGAAGGACTA TTAAACAAGC CCAGAAGTAA ACTATCCCTG 
AGAGGAGGCA GACCTGATTA CTGCTTAGAA AATGTCCAAC TCAAATTTGA AAGAGATGAG 
AAGACCCTTA TGACTATATG GCAGAATACA TCTTCAGCCA TCAACCTCTT GGTAAATTCT 
GAAATACAGT TGAGGAAACT AGAAGATGAT AAAGAAAACA AACCAACATC AACTAAAGAA 
AAATCTCCTC AAGATGGTGG CAAAGGTCCT AAAGGTGCTA AGAAAAAGCC AGATAAGAAG 
AAAGTTCCAT CACCAGTTGA AGCCGTTTCT ATGGTTCTAA CACCAGAAGA ACAGGAGGCT 
CTTGATAAGA GGAATGAATT GAAAATGAAA ATAAAGGAAG AACATATTAC AGCTCTTCAG 
TGTGAAGCAC AAGCTTCAAT ATTCCGACTG GAACTGATCA AGATGAAGGC CTTATCTTAC 
ATGGAAGAGC TGCTAGGAAG AGCAGTTGAG GTTTACAAAC TGATGGACAA ATGGCTCGGA 
GCCCGATACT TAGCTGAAAT GGCAAGTGTG GAAAAATTAA CTGAAGTGGC TCACTACCAC 
ATTGAAACCT CAACAAGGAT TCAGAATGAA CTTATTTTAA ATGAAGAAGA TTTCTACATC 
AATGGTGATG TGAAAGTCTT TCCAGATCCG CTTCCTCCCG TCCGGCCTCC ACCTGTGGAG 
AAAGAAATGA ATACTTTTCT CACTCTTGAA CAACTCAACA CCCTTCGAAA TGAGTTTCTC 
CAGATGGCAC CAAAAGCCAA AACACCATGG AAGTATTTTA AAAAAGGAAT CTCTTCAGTT 
GATACTAGAA GACATGCTGA GGAAAAGAAA AGGCTAAGAA ATCTTGACTA TTACCTACCT 
AAGAGGAACA ACCAATTTTT GAAAATTTGG CTAAAAAAAT ACCCTTGGCT CGTGTATGAT 
GACAGGTCAG ACTTAATGTT CTGTGGCTTG TGTCGCAAAC ATGGAGTGAG CTCAGGAGGA 
AACCATGTTA GCTTTTTTTA TGGCACCAAC AACTTTAGGA CTGAATTTCT CCAAGCACAT 
GACAATAGTG AGGCACACAC GAAGGCTGTT CTAGCAGAAG CAACAGGGGT TTCTTCCCTG 
ACTGATGCTG CCAAGGAGCA GATTCTAAAG AACAGGAACA AAGTAATCTT GGGAAGAATA 
GAAAACCTCT TTAGAACATG TCATGCTATT GCCAAGACTG GACGTCCCCT CAAAGACTTT 
GTCTGGATGT GTAAACTGGA TGATATGAAG GGTGTAGACG TTGGCCCAAT ATTCAGGACT 
GACAAGTCAG CCAGAACTTT CATATACTTT ATTGCTGAAG TAGAACGCAT ATGTCTTCGA 
GAGAAATTAG AAAAATGTAA GTTCTTCTCA CTCATCAGTG ATGGTGGCTC AGGCAGCTTC 
ATAGAAGACA CCAAAGTTGT TTACATCCAG TTTGCATATG AGGGCAGGGT CTGCTGTCAG 
GTTGTTGGAG TACAGACTGT GGACAAAATT GAACCGGTGG CAATAAAAAG TGCCATTGAA 
AAAACACTTG AGATAAATCT TCAACTAAAT CTGTCTAGCA AAGACTGGTC CAAAAAACTC 
GTTGGGTTTG GAAGTGATGG TGCATCTGTC ATGGTGGGAG AGAACAATGG TGTAGCCAGG 
CTTCTGAAAG AAATTCAGCC ATGTGTGCAA ACAGTCCACT GCTTTGCACA TAGACTTGAA 
CTAGCCTACC AAGAAGCATT GCAGAGCATC CAATTATACA GTACTATGAA TGATCTTCTC 
CAGAATATAT ATTATTTTTA TCATGATTCA CCTTCAAATA CAAAAGCTCT GATAGCTGCC 
TTTGAAGACC TTAAAATCCG CCCTGTGATT CCCTCTCGAG TTGGCAACAA CAGATGGCTC 
CCTCGGTTAC AGATGGCTCT ACAAATCTTT CTCAAAGGAT ATTCTCCAAT TGTCAAGCAA 
TTAAGAACAA TGCATGAAGG CAGAAGTTCC GCTGATCAAC AGAAAGCTCA AGAGCTCCTA 
CAGTTCCTTC TCCAAGCTGA GGTTGTCAGA TTTGCTCACT TCTTGCTGGA TGTAATCAAC 
GTCCTCAGCA TTTTGTCTCG AGTCAATCAG GACAGAAATT CATCAATAGG AGATGTATTT 
GCAACCCTAC AATCAACACT AGGAACACTC AAGATGTATA AAAAAAGACC AGGTCCAAAG 
GAACGTCTGA CAGAGTCAGC CACACACTTT CATGGTAACC AACTGACAGG AAATGGAAAT 
ATCTCTGAAG TACGAAACAC AGTCTTAACA AATCTCTCAA AGCATCTTCA TGATTGTTTC 
AGTGATGCCA GCCATGATGT GGTGAGGGCG ACCACTATTG GAAGTTTCAA ATTATGGCCA 
AGAAAGCTAG ATCAAGAGTT TGGTGAGAAA GAGGTAACAA TTCTTACAAA GCATTATGAA 
CCTGTGTTGG AAGGGGCCAA TGTGAACATT GATGAAGTGG ACACAGAATG GAGCATGATG 
AAATTAGAGA TTTATAATCG ATTTCAGAAC ATCCGAAAGT TAACCTGGGA TATTGTGAAT 
TCCATCTACT CATACAAGTA TCCCAATATA TTGACCCTAG TGGATCTGGT GCTGACACTC 
CCTGCAAGTT CAGCTGAGGC GGAACGTGGG TTCAGTCAGA TGAAACTAGC CAAGTCACAA 
ATGCATTTTA AAACAGATCC TGAGAACATG ACAGACATTT TAATAATCCA GTTGAATTCT 
CCAGACATCA ATAAGTTTGA TCCCAAGAAA GCCATCCATC TATGGAATAC TTCTTGGCAG 
AGAAATACAC TTCCACCAAT TGACCACACA ATAACCAAGT CAGATTGCTC CTCTAGCTCA 
GATAGTCTTT ATGAAAGTGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016287092.1
CDS	84..6527
Translation	MSDILCRWLNQEVKLSRTVSSKGIEKDFSNGFLLGELLHKYELQDDFPLFSEGRVSNAKLNNFSRLEPTFQLLGVQFDQNVAQNIMTEKHGSVTILLYQLYIALQKKRKSGLTGVAMQTMRPLAPAKLQTMRSEIYRERLKNLVPRQTDLNLSRVSEKFEAKYKKMEEKIARVHFQNIRKFEKFQEEQRIQDLEKQRLGRRRQHEMMARIQAAILQIPKTVPNRTLKSIESHKMMMKKKEEEDVLNEIQKFEMLIKKELEAKGSGSKVPSAAQTTADLLSTYSDEEYIKKIQKRLEEDTLAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRVFREKQYEERRLKDFQDALDREAALARQAKLEYEEQAQKEIEIHERIAAERSQARYKKHYSMCTEILDNIIDLSTKIAEYRELTHNLIPVKIMRDWKELFFSGKPIYEIASIDSKPSRFTSEQLIELSKRNLLDDNDYEEYKNLTGEWVLPEDMADVPLPSNNNILGHVIRVVHEKVYPPKPAYIATQLPSSSIKGCMLGKTCSGKTSCLKILEEVFHIEILCMDTLVQEAIQAFLRNETVTEVETHKEDLSKEVQDIKDSNMTTIAEIIPTEEKKDENLPQQDEVIIKPPSAQDVRIRLTNRAQLGSKCEKLLKRGRSIPDVLLIQIMVNAINEISSDKGWILDGFPMTINQAKLLEEALTGFNTDQPEHEIKAHKKSTLVFDPSAPKDPPPPPPAFDFVMLLDITDTLVLNRVMGEEKRNTDTTGIPESATSEMFPEEQEKFELLRDQIQHRILGFLDNWPELEKWFSGKQDILITVNAEVDKRALATKLQELFIAELRKKEAKAKQELEEQEEERKKKEALPPEPPPPPPPEPEEEPKEAKGKGKGKGKGAKEGKGKKDTSPKGKDAAKEAKAKKSETSPKGKGRSPDKRSPEDKTSPPAPPGPPPVKAGSEDWVYVDEPLPQEIFEYLDTFWEVIESSYINTSKAIFRQIRDERHSVLYYLHNVRLNYQGYLKRPDHKQEFVSQWQEDFNNLPEDLWEDEETKAELHQRVNDLRDRLWDICDTRKEEAEQERADIIDESWLQDHLGILMNHFFSLMQVELSRFQDIKTFLQDYYRGMEGKIPTDLAKSGYTRVPLIDLDEEDTDITSKIPVIPRRQPSPEGLLNKPRSKLSLRGGRPDYCLENVQLKFERDEKTLMTIWQNTSSAINLLVNSEIQLRKLEDDKENKPTSTKEKSPQDGGKGPKGAKKKPDKKKVPSPVEAVSMVLTPEEQEALDKRNELKMKIKEEHITALQCEAQASIFRLELIKMKALSYMEELLGRAVEVYKLMDKWLGARYLAEMASVEKLTEVAHYHIETSTRIQNELILNEEDFYINGDVKVFPDPLPPVRPPPVEKEMNTFLTLEQLNTLRNEFLQMAPKAKTPWKYFKKGISSVDTRRHAEEKKRLRNLDYYLPKRNNQFLKIWLKKYPWLVYDDRSDLMFCGLCRKHGVSSGGNHVSFFYGTNNFRTEFLQAHDNSEAHTKAVLAEATGVSSLTDAAKEQILKNRNKVILGRIENLFRTCHAIAKTGRPLKDFVWMCKLDDMKGVDVGPIFRTDKSARTFIYFIAEVERICLREKLEKCKFFSLISDGGSGSFIEDTKVVYIQFAYEGRVCCQVVGVQTVDKIEPVAIKSAIEKTLEINLQLNLSSKDWSKKLVGFGSDGASVMVGENNGVARLLKEIQPCVQTVHCFAHRLELAYQEALQSIQLYSTMNDLLQNIYYFYHDSPSNTKALIAAFEDLKIRPVIPSRVGNNRWLPRLQMALQIFLKGYSPIVKQLRTMHEGRSSADQQKAQELLQFLLQAEVVRFAHFLLDVINVLSILSRVNQDRNSSIGDVFATLQSTLGTLKMYKKRPGPKERLTESATHFHGNQLTGNGNISEVRNTVLTNLSKHLHDCFSDASHDVVRATTIGSFKLWPRKLDQEFGEKEVTILTKHYEPVLEGANVNIDEVDTEWSMMKLEIYNRFQNIRKLTWDIVNSIYSYKYPNILTLVDLVLTLPASSAEAERGFSQMKLAKSQMHFKTDPENMTDILIIQLNSPDINKFDPKKAIHLWNTSWQRNTLPPIDHTITKSDCSSSSDSLYESD

Target ORF information:

RefSeq Version	XM_016431606.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016431606.1

ORF Insert Sequence:

ATGTCGGACA TCCTGTGCAG GTGGCTAAAC CAGGAGGTGA AGCTGTCCCG GACAGTGAGT 
TCAAAGGGGA TTGAAAAAGA TTTTTCTAAT GGCTTCCTAC TGGGTGAACT TCTGCACAAA 
TATGAACTTC AGGATGACTT TCCTCTGTTT TCAGAAGGCA GGGTTTCCAA TGCAAAACTT 
AATAATTTTT CTCGCTTAGA ACCAACATTT CAACTCCTGG GTGTTCAGTT CGATCAAAAT 
GTGGCCCAGA ATATTATGAC AGAAAAGCAT GGCTCTGTAA CCATACTTCT TTATCAGTTG 
TACATTGCGC TTCAAAAAAA GAGAAAAAGT GGACTCACGG GAGTAGCAAT GCAAACAATG 
AGACCTTTAG CACCTGCAAA GCTACAAACT ATGAGAAGTG AGATTTATCG AGAGAGACTT 
AAGAACCTGG TGCCTCGGCA AACTGATTTG AATCTAAGTC GGGTTTCAGA AAAGTTTGAA 
GCAAAGTATA AAAAAATGGA AGAAAAAATT GCTCGTGTTC ATTTTCAAAA TATCCGAAAA 
TTTGAAAAGT TCCAGGAAGA ACAAAGAATT CAAGATCTTG AAAAGCAGCG CCTGGGAAGA 
AGACGACAAC ATGAAATGAT GGCTAGAATC CAAGCAGCCA TTTTACAGAT TCCCAAAACA 
GTACCCAATC GTACTCTGAA ATCTATTGAG AGCCACAAAA TGATGATGAA GAAAAAAGAG 
GAAGAAGATG TGCTGAATGA AATTCAGAAG TTTGAAATGC TAATAAAAAA GGAGCTTGAG 
GCCAAAGGCA GTGGATCAAA AGTTCCTTCG GCAGCCCAGA CAACTGCTGA CTTGTTAAGC 
ACGTATTCAG ATGAGGAATA CATTAAAAAA ATTCAAAAAC GTCTAGAGGA AGATACCTTA 
GCCCGGGAAC AAAGGGAGAA AAGACGCAGA AAATTGTTAA TGGATCAATT GATAGCCCAT 
GAAGCTCAAG AGGAGGCTTA CCGAGAAGAA CAACTCATTA ACCGACTAAT GAGACAATCT 
CAGCAGGAAC GTCGGATTGC TGTGCAACTC ATGCATGTTC GACACGAAAA GGAAGTTTTA 
TGGCAAAACA GGGTTTTCAG AGAAAAACAA TATGAAGAAA GACGGCTGAA AGATTTCCAG 
GATGCTCTTG ACAGAGAAGC AGCTCTTGCA AGACAAGCCA AGCTGGAGTA TGAAGAACAA 
GCCCAAAAGG AAATAGAAAT TCATGAAAGA ATAGCAGCAG AAAGATCTCA AGCTCGTTAT 
AAAAAGCATT ATTCAATGTG CACAGAAATC TTGGACAATA TCATTGATTT ATCAACTAAA 
ATAGCAGAAT ATCGAGAATT AACACATAAT CTTATTCCAG TTAAAATTAT GCGTGATTGG 
AAGGAGCTGT TTTTCAGTGG AAAGCCAATT TATGAAATAG CCTCCATTGA CTCCAAGCCA 
TCTCGTTTTA CCTCAGAACA ACTAATTGAG CTATCCAAAA GGAATTTACT AGATGACAAT 
GATTATGAGG AGTACAAGAA CTTGACAGGA GAGTGGGTCC TCCCAGAAGA TATGGCTGAT 
GTTCCCCTAC CTTCCAACAA TAACATATTG GGTCATGTGA TTCGAGTAGT TCATGAAAAA 
GTTTACCCTC CAAAACCAGC ATATATTGCA ACTCAGTTAC CTTCATCTTC GATTAAAGGG 
TGCATGCTGG GCAAGACATG CAGTGGGAAA ACCAGCTGTC TAAAGATTTT AGAAGAAGTT 
TTCCATATTG AGATACTTTG TATGGATACT CTGGTTCAAG AGGCTATTCA GGCTTTCCTT 
AGAAATGAAA CTGTAACTGA GGTGGAAACA CATAAAGAAG ATTTATCAAA AGAAGTTCAA 
GATATTAAGG ATTCAAACAT GACGACTATT GCAGAAATAA TTCCTACTGA GGAGAAAAAA 
GATGAGAATC TACCACAACA AGATGAAGTG ATTATTAAAC CACCATCAGC CCAGGATGTG 
AGAATAAGAC TTACTAATCG TGCTCAACTT GGTTCAAAAT GTGAAAAGTT GCTCAAGAGA 
GGAAGAAGTA TCCCCGATGT ACTGCTAATT CAAATCATGG TGAATGCTAT TAATGAAATA 
TCATCAGACA AAGGATGGAT CTTGGATGGT TTTCCAATGA CCATAAATCA AGCAAAACTT 
CTTGAGGAAG CCCTTACAGG GTTCAACACA GATCAACCAG AACATGAAAT AAAGGCTCAC 
AAAAAATCAA CATTGGTTTT TGATCCATCT GCTCCCAAAG ACCCCCCTCC TCCCCCACCT 
GCATTTGATT TTGTTATGTT GCTGGATATA ACTGACACAT TGGTGCTGAA TCGTGTGATG 
GGAGAGGAGA AAAGAAACAC TGACACAACT GGTATACCAG AAAGTGCAAC TTCAGAAATG 
TTTCCTGAAG AACAAGAAAA GTTTGAACTT TTGAGGGATC AGATACAACA CAGAATTTTA 
GGTTTCTTGG ACAACTGGCC AGAGCTGGAA AAATGGTTTT CAGGAAAACA AGACATTCTA 
ATCACAGTCA ATGCAGAAGT AGATAAAAGG GCTTTAGCCA CAAAACTACA AGAGCTTTTT 
ATTGCTGAAC TGAGGAAAAA AGAAGCTAAA GCAAAACAGG AATTAGAAGA ACAGGAAGAG 
GAAAGGAAAA AGAAAGAAGC CCTCCCACCT GAACCGCCAC CTCCACCTCC CCCTGAACCA 
GAAGAAGAGC CAAAGGAAGC AAAAGGGAAA GGAAAAGGGA AAGGTAAAGG TGCTAAAGAA 
GGAAAAGGAA AGAAAGACAC TTCTCCCAAG GGCAAAGATG CTGCTAAAGA AGCAAAAGCA 
AAGAAAAGTG AAACTTCTCC AAAAGGCAAA GGAAGGTCAC CAGACAAGAG GTCACCTGAA 
GATAAAACAT CACCTCCTGC ACCTCCAGGA CCACCACCAG TCAAAGCAGG ATCAGAAGAC 
TGGGTCTATG TGGATGAGCC ACTTCCTCAG GAAATATTTG AATATCTAGA CACTTTTTGG 
GAAGTAATAG AAAGTTCATA CATAAATACC AGCAAAGCTA TATTCAGACA GATAAGAGAT 
GAGAGACATA GTGTACTTTA TTATCTACAT AATGTCAGAC TAAATTACCA GGGATATCTG 
AAACGGCCAG ATCACAAGCA GGAATTTGTA TCACAATGGC AGGAGGATTT TAACAACCTC 
CCTGAAGACT TATGGGAGGA TGAGGAAACA AAAGCTGAAC TACACCAAAG AGTCAATGAT 
TTAAGGGACC GCCTGTGGGA TATATGTGAT ACTCGAAAGG AAGAGGCAGA ACAAGAACGT 
GCTGATATCA TTGATGAATC CTGGTTACAA GATCATCTTG GAATTCTTAT GAATCATTTC 
TTTTCTTTGA TGCAGGTGGA ACTTAGCCGT TTCCAGGACA TAAAGACTTT TCTACAAGAT 
TACTATAGGG GAATGGAAGG AAAAATCCCC ACCGACCTTG CTAAATCTGG ATATACTCGA 
GTTCCCCTTA TAGATCTTGA TGAAGAGGAT ACCGATATCA CCTCCAAAAT CCCTGTAATT 
CCTCGGAGAC AGCCTTCTCC TGAAGGACTA TTAAACAAGC CCAGAAGTAA ACTATCCCTG 
AGAGGAGGCA GACCTGATTA CTGCTTAGAA AATGTCCAAC TCAAATTTGA AAGAGATGAG 
AAGACCCTTA TGACTATATG GCAGAATACA TCTTCAGCCA TCAACCTCTT GGTAAATTCT 
GAAATACAGT TGAGGAAACT AGAAGATGAT AAAGAAAACA AACCAACATC AACTAAAGAA 
AAATCTCCTC AAGATGGTGG CAAAGGTCCT AAAGGTGCTA AGAAAAAGCC AGATAAGAAG 
AAAGTTCCAT CACCAGTTGA AGCCGTTTCT ATGGTTCTAA CACCAGAAGA ACAGGAGGCT 
CTTGATAAGA GGAATGAATT GAAAATGAAA ATAAAGGAAG AACATATTAC AGCTCTTCAG 
TGTGAAGCAC AAGCTTCAAT ATTCCGACTG GAACTGATCA AGATGAAGGC CTTATCTTAC 
ATGGAAGAGC TGCTAGGAAG AGCAGTTGAG GTTTACAAAC TGATGGACAA ATGGCTCGGA 
GCCCGATACT TAGCTGAAAT GGCAAGTGTG GAAAAATTAA CTGAAGTGGC TCACTACCAC 
ATTGAAACCT CAACAAGGAT TCAGAATGAA CTTATTTTAA ATGAAGAAGA TTTCTACATC 
AATGGTGATG TGAAAGTCTT TCCAGATCCG CTTCCTCCCG TCCGGCCTCC ACCTGTGGAG 
AAAGAAATGA ATACTTTTCT CACTCTTGAA CAACTCAACA CCCTTCGAAA TGAGTTTCTC 
CAGATGGCAC CAAAAGCCAA AACACCATGG AAGTATTTTA AAAAAGGAAT CTCTTCAGTT 
GATACTAGAA GACATGCTGA GGAAAAGAAA AGGCTAAGAA ATCTTGACTA TTACCTACCT 
AAGAGGAACA ACCAATTTTT GAAAATTTGG CTAAAAAAAT ACCCTTGGCT CGTGTATGAT 
GACAGGTCAG ACTTAATGTT CTGTGGCTTG TGTCGCAAAC ATGGAGTGAG CTCAGGAGGA 
AACCATGTTA GCTTTTTTTA TGGCACCAAC AACTTTAGGA CTGAATTTCT CCAAGCACAT 
GACAATAGTG AGGCACACAC GAAGGCTGTT CTAGCAGAAG CAACAGGGGT TTCTTCCCTG 
ACTGATGCTG CCAAGGAGCA GATTCTAAAG AACAGGAACA AAGTAATCTT GGGAAGAATA 
GAAAACCTCT TTAGAACATG TCATGCTATT GCCAAGACTG GACGTCCCCT CAAAGACTTT 
GTCTGGATGT GTAAACTGGA TGATATGAAG GGTGTAGACG TTGGCCCAAT ATTCAGGACT 
GACAAGTCAG CCAGAACTTT CATATACTTT ATTGCTGAAG TAGAACGCAT ATGTCTTCGA 
GAGAAATTAG AAAAATGTAA GTTCTTCTCA CTCATCAGTG ATGGTGGCTC AGGCAGCTTC 
ATAGAAGACA CCAAAGTTGT TTACATCCAG TTTGCATATG AGGGCAGGGT CTGCTGTCAG 
GTTGTTGGAG TACAGACTGT GGACAAAATT GAACCGGTGG CAATAAAAAG TGCCATTGAA 
AAAACACTTG AGATAAATCT TCAACTAAAT CTGTCTAGCA AAGACTGGTC CAAAAAACTC 
GTTGGGTTTG GAAGTGATGG TGCATCTGTC ATGGTGGGAG AGAACAATGG TGTAGCCAGG 
CTTCTGAAAG AAATTCAGCC ATGTGTGCAA ACAGTCCACT GCTTTGCACA TAGACTTGAA 
CTAGCCTACC AAGAAGCATT GCAGAGCATC CAATTATACA GTACTATGAA TGATCTTCTC 
CAGAATATAT ATTATTTTTA TCATGATTCA CCTTCAAATA CAAAAGCTCT GATAGCTGCC 
TTTGAAGACC TTAAAATCCG CCCTGTGATT CCCTCTCGAG TTGGCAACAA CAGATGGCTC 
CCTCGGTTAC AGATGGCTCT ACAAATCTTT CTCAAAGGAT ATTCTCCAAT TGTCAAGCAA 
TTAAGAACAA TGCATGAAGG CAGAAGTTCC GCTGATCAAC AGAAAGCTCA AGAGCTCCTA 
CAGTTCCTTC TCCAAGCTGA GGTTGTCAGA TTTGCTCACT TCTTGCTGGA TGTAATCAAC 
GTCCTCAGCA TTTTGTCTCG AGTCAATCAG GACAGAAATT CATCAATAGG AGATGTATTT 
GCAACCCTAC AATCAACACT AGGAACACTC AAGATGTATA AAAAAAGACC AGGTCCAAAG 
GAACGTCTGA CAGAGTCAGC CACACACTTT CATGGTAACC AACTGACAGG AAATGGAAAT 
ATCTCTGAAG TACGAAACAC AGTCTTAACA AATCTCTCAA AGCATCTTCA TGATTGTTTC 
AGTGATGCCA GCCATGATGT GGTGAGGGCG ACCACTATTG GAAGTTTCAA ATTATGGCCA 
AGAAAGCTAG ATCAAGAGTT TGGTGAGAAA GAGGTAACAA TTCTTACAAA GCATTATGAA 
CCTGTGTTGG AAGGGGCCAA TGTGAACATT GATGAAGTGG ACACAGAATG GAGCATGATG 
AAATTAGAGA TTTATAATCG ATTTCAGAAC ATCCGAAAGT TAACCTGGGA TATTGTGAAT 
TCCATCTACT CATACAAGTA TCCCAATATA TTGACCCTAG TGGATCTGGT GCTGACACTC 
CCTGCAAGTT CAGCTGAGGC GGAACGTGGG TTCAGTCAGA TGAAACTAGC CAAGTCACAA 
ATGCATTTTA AAACAGATCC TGAGAACATG ACAGACATTT TAATAATCCA GTTGAATTCT 
CCAGACATCA ATAAGTTTGA TCCCAAGAAA GCCATCCATC TATGGAATAC TTCTTGGCAG 
AGAAATACAC TTCCACCAAT TGACCACACA ATAACCAAGT CAGATTGCTC CTCTAGCTCA 
GATAGTCTTT ATGAAAGTGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_016431607.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016287093.1 Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.

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CloneID	OMc41042
Clone ID Related Accession (Same CDS sequence)	XM_016431607.1
Accession Version	XM_016431607.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	sperm flagellar protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008803.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACA TCCTGTGCAG GTGGCTAAAC CAGGAGGTGA AGCTGTCCCG GACAGTGAGT 
TCAAAGGGGA TTGAAAAAGA TTTTTCTAAT GGCTTCCTAC TGGGTGAACT TCTGCACAAA 
TATGAACTTC AGGATGACTT TCCTCTGTTT TCAGAAGGCA GGGTTTCCAA TGCAAAACTT 
AATAATTTTT CTCGCTTAGA ACCAACATTT CAACTCCTGG GTGTTCAGTT CGATCAAAAT 
GTGGCCCAGA ATATTATGAC AGAAAAGCAT GGCTCTGTAA CCATACTTCT TTATCAGTTG 
TACATTGCGC TTCAAAAAAA GAGAAAAAGT GGACTCACGG GAGTAGCAAT GCAAACAATG 
AGACCTTTAG CACCTGCAAA GCTACAAACT ATGAGAAGTG AGATTTATCG AGAGAGACTT 
AAGAACCTGG TGCCTCGGCA AACTGATTTG AATCTAAGTC GGGTTTCAGA AAAGTTTGAA 
GCAAAGTATA AAAAAATGGA AGAAAAAATT GCTCGTGTTC ATTTTCAAAA TATCCGAAAA 
TTTGAAAAGT TCCAGGAAGA ACAAAGAATT CAAGATCTTG AAAAGCAGCG CCTGGGAAGA 
AGACGACAAC ATGAAATGAT GGCTAGAATC CAAGCAGCCA TTTTACAGAT TCCCAAAACA 
GTACCCAATC GTACTCTGAA ATCTATTGAG AGCCACAAAA TGATGATGAA GAAAAAAGAG 
GAAGAAGATG TGCTGAATGA AATTCAGAAG TTTGAAATGC TAATAAAAAA GGAGCTTGAG 
GCCAAAGGCA GTGGATCAAA AGTTCCTTCG GCAGCCCAGA CAACTGCTGA CTTGTTAAGC 
ACGTATTCAG ATGAGGAATA CATTAAAAAA ATTCAAAAAC GTCTAGAGGA AGATACCTTA 
GCCCGGGAAC AAAGGGAGAA AAGACGCAGA AAATTGTTAA TGGATCAATT GATAGCCCAT 
GAAGCTCAAG AGGAGGCTTA CCGAGAAGAA CAACTCATTA ACCGACTAAT GAGACAATCT 
CAGCAGGAAC GTCGGATTGC TGTGCAACTC ATGCATGTTC GACACGAAAA GGAAGTTTTA 
TGGCAAAACA GGGTTTTCAG AGAAAAACAA TATGAAGAAA GACGGCTGAA AGATTTCCAG 
GATGCTCTTG ACAGAGAAGC AGCTCTTGCA AGACAAGCCA AGCTGGAGTA TGAAGAACAA 
GCCCAAAAGG AAATAGAAAT TCATGAAAGA ATAGCAGCAG AAAGATCTCA AGCTCGTTAT 
AAAAAGCATT ATTCAATGTG CACAGAAATC TTGGACAATA TCATTGATTT ATCAACTAAA 
ATAGCAGAAT ATCGAGAATT AACACATAAT CTTATTCCAG TTAAAATTAT GCGTGATTGG 
AAGGAGCTGT TTTTCAGTGG AAAGCCAATT TATGAAATAG CCTCCATTGA CTCCAAGCCA 
TCTCGTTTTA CCTCAGAACA ACTAATTGAG CTATCCAAAA GGAATTTACT AGATGACAAT 
GATTATGAGG AGTACAAGAA CTTGACAGGA GAGTGGGTCC TCCCAGAAGA TATGGCTGAT 
GTTCCCCTAC CTTCCAACAA TAACATATTG GGTCATGTGA TTCGAGTAGT TCATGAAAAA 
GTTTACCCTC CAAAACCAGC ATATATTGCA ACTCAGTTAC CTTCATCTTC GATTAAAGGG 
TGCATGCTGG GCAAGACATG CAGTGGGAAA ACCAGCTGTC TAAAGATTTT AGAAGAAGTT 
TTCCATATTG AGATACTTTG TATGGATACT CTGGTTCAAG AGGCTATTCA GGCTTTCCTT 
AGAAATGAAA CTGTAACTGA GGTGGAAACA CATAAAGAAG ATTTATCAAA AGAAGTTCAA 
GATATTAAGG ATTCAAACAT GACGACTATT GCAGAAATAA TTCCTACTGA GGAGAAAAAA 
GATGAGAATC TACCACAACA AGATGAAGTG ATTATTAAAC CACCATCAGC CCAGGATGTG 
AGAATAAGAC TTACTAATCG TGCTCAACTT GGTTCAAAAT GTGAAAAGTT GCTCAAGAGA 
GGAAGAAGTA TCCCCGATGT ACTGCTAATT CAAATCATGG TGAATGCTAT TAATGAAATA 
TCATCAGACA AAGGATGGAT CTTGGATGGT TTTCCAATGA CCATAAATCA AGCAAAACTT 
CTTGAGGAAG CCCTTACAGG GTTCAACACA GATCAACCAG AACATGAAAT AAAGGCTCAC 
AAAAAATCAA CATTGGTTTT TGATCCATCT GCTCCCAAAG ACCCCCCTCC TCCCCCACCT 
GCATTTGATT TTGTTATGTT GCTGGATATA ACTGACACAT TGGTGCTGAA TCGTGTGATG 
GGAGAGGAGA AAAGAAACAC TGACACAACT GGTATACCAG AAAGTGCAAC TTCAGAAATG 
TTTCCTGAAG AACAAGAAAA GTTTGAACTT TTGAGGGATC AGATACAACA CAGAATTTTA 
GGTTTCTTGG ACAACTGGCC AGAGCTGGAA AAATGGTTTT CAGGAAAACA AGACATTCTA 
ATCACAGTCA ATGCAGAAGT AGATAAAAGG GCTTTAGCCA CAAAACTACA AGAGCTTTTT 
ATTGCTGAAC TGAGGAAAAA AGAAGCTAAA GCAAAACAGG AATTAGAAGA ACAGGAAGAG 
GAAAGGAAAA AGAAAGAAGC CCTCCCACCT GAACCGCCAC CTCCACCTCC CCCTGAACCA 
GAAGAAGAGC CAAAGGAAGC AAAAGGGAAA GGAAAAGGGA AAGGTAAAGG TGCTAAAGAA 
GGAAAAGGAA AGAAAGACAC TTCTCCCAAG GGCAAAGATG CTGCTAAAGA AGCAAAAGCA 
AAGAAAAGTG AAACTTCTCC AAAAGGAAGG TCACCAGACA AGAGGTCACC TGAAGATAAA 
ACATCACCTC CTGCACCTCC AGGACCACCA CCAGTCAAAG CAGGATCAGA AGACTGGGTC 
TATGTGGATG AGCCACTTCC TCAGGAAATA TTTGAATATC TAGACACTTT TTGGGAAGTA 
ATAGAAAGTT CATACATAAA TACCAGCAAA GCTATATTCA GACAGATAAG AGATGAGAGA 
CATAGTGTAC TTTATTATCT ACATAATGTC AGACTAAATT ACCAGGGATA TCTGAAACGG 
CCAGATCACA AGCAGGAATT TGTATCACAA TGGCAGGAGG ATTTTAACAA CCTCCCTGAA 
GACTTATGGG AGGATGAGGA AACAAAAGCT GAACTACACC AAAGAGTCAA TGATTTAAGG 
GACCGCCTGT GGGATATATG TGATACTCGA AAGGAAGAGG CAGAACAAGA ACGTGCTGAT 
ATCATTGATG AATCCTGGTT ACAAGATCAT CTTGGAATTC TTATGAATCA TTTCTTTTCT 
TTGATGCAGG TGGAACTTAG CCGTTTCCAG GACATAAAGA CTTTTCTACA AGATTACTAT 
AGGGGAATGG AAGGAAAAAT CCCCACCGAC CTTGCTAAAT CTGGATATAC TCGAGTTCCC 
CTTATAGATC TTGATGAAGA GGATACCGAT ATCACCTCCA AAATCCCTGT AATTCCTCGG 
AGACAGCCTT CTCCTGAAGG ACTATTAAAC AAGCCCAGAA GTAAACTATC CCTGAGAGGA 
GGCAGACCTG ATTACTGCTT AGAAAATGTC CAACTCAAAT TTGAAAGAGA TGAGAAGACC 
CTTATGACTA TATGGCAGAA TACATCTTCA GCCATCAACC TCTTGGTAAA TTCTGAAATA 
CAGTTGAGGA AACTAGAAGA TGATAAAGAA AACAAACCAA CATCAACTAA AGAAAAATCT 
CCTCAAGATG GTGGCAAAGG TCCTAAAGGT GCTAAGAAAA AGCCAGATAA GAAGAAAGTT 
CCATCACCAG TTGAAGCCGT TTCTATGGTT CTAACACCAG AAGAACAGGA GGCTCTTGAT 
AAGAGGAATG AATTGAAAAT GAAAATAAAG GAAGAACATA TTACAGCTCT TCAGTGTGAA 
GCACAAGCTT CAATATTCCG ACTGGAACTG ATCAAGATGA AGGCCTTATC TTACATGGAA 
GAGCTGCTAG GAAGAGCAGT TGAGGTTTAC AAACTGATGG ACAAATGGCT CGGAGCCCGA 
TACTTAGCTG AAATGGCAAG TGTGGAAAAA TTAACTGAAG TGGCTCACTA CCACATTGAA 
ACCTCAACAA GGATTCAGAA TGAACTTATT TTAAATGAAG AAGATTTCTA CATCAATGGT 
GATGTGAAAG TCTTTCCAGA TCCGCTTCCT CCCGTCCGGC CTCCACCTGT GGAGAAAGAA 
ATGAATACTT TTCTCACTCT TGAACAACTC AACACCCTTC GAAATGAGTT TCTCCAGATG 
GCACCAAAAG CCAAAACACC ATGGAAGTAT TTTAAAAAAG GAATCTCTTC AGTTGATACT 
AGAAGACATG CTGAGGAAAA GAAAAGGCTA AGAAATCTTG ACTATTACCT ACCTAAGAGG 
AACAACCAAT TTTTGAAAAT TTGGCTAAAA AAATACCCTT GGCTCGTGTA TGATGACAGG 
TCAGACTTAA TGTTCTGTGG CTTGTGTCGC AAACATGGAG TGAGCTCAGG AGGAAACCAT 
GTTAGCTTTT TTTATGGCAC CAACAACTTT AGGACTGAAT TTCTCCAAGC ACATGACAAT 
AGTGAGGCAC ACACGAAGGC TGTTCTAGCA GAAGCAACAG GGGTTTCTTC CCTGACTGAT 
GCTGCCAAGG AGCAGATTCT AAAGAACAGG AACAAAGTAA TCTTGGGAAG AATAGAAAAC 
CTCTTTAGAA CATGTCATGC TATTGCCAAG ACTGGACGTC CCCTCAAAGA CTTTGTCTGG 
ATGTGTAAAC TGGATGATAT GAAGGGTGTA GACGTTGGCC CAATATTCAG GACTGACAAG 
TCAGCCAGAA CTTTCATATA CTTTATTGCT GAAGTAGAAC GCATATGTCT TCGAGAGAAA 
TTAGAAAAAT GTAAGTTCTT CTCACTCATC AGTGATGGTG GCTCAGGCAG CTTCATAGAA 
GACACCAAAG TTGTTTACAT CCAGTTTGCA TATGAGGGCA GGGTCTGCTG TCAGGTTGTT 
GGAGTACAGA CTGTGGACAA AATTGAACCG GTGGCAATAA AAAGTGCCAT TGAAAAAACA 
CTTGAGATAA ATCTTCAACT AAATCTGTCT AGCAAAGACT GGTCCAAAAA ACTCGTTGGG 
TTTGGAAGTG ATGGTGCATC TGTCATGGTG GGAGAGAACA ATGGTGTAGC CAGGCTTCTG 
AAAGAAATTC AGCCATGTGT GCAAACAGTC CACTGCTTTG CACATAGACT TGAACTAGCC 
TACCAAGAAG CATTGCAGAG CATCCAATTA TACAGTACTA TGAATGATCT TCTCCAGAAT 
ATATATTATT TTTATCATGA TTCACCTTCA AATACAAAAG CTCTGATAGC TGCCTTTGAA 
GACCTTAAAA TCCGCCCTGT GATTCCCTCT CGAGTTGGCA ACAACAGATG GCTCCCTCGG 
TTACAGATGG CTCTACAAAT CTTTCTCAAA GGATATTCTC CAATTGTCAA GCAATTAAGA 
ACAATGCATG AAGGCAGAAG TTCCGCTGAT CAACAGAAAG CTCAAGAGCT CCTACAGTTC 
CTTCTCCAAG CTGAGGTTGT CAGATTTGCT CACTTCTTGC TGGATGTAAT CAACGTCCTC 
AGCATTTTGT CTCGAGTCAA TCAGGACAGA AATTCATCAA TAGGAGATGT ATTTGCAACC 
CTACAATCAA CACTAGGAAC ACTCAAGATG TATAAAAAAA GACCAGGTCC AAAGGAACGT 
CTGACAGAGT CAGCCACACA CTTTCATGGT AACCAACTGA CAGGAAATGG AAATATCTCT 
GAAGTACGAA ACACAGTCTT AACAAATCTC TCAAAGCATC TTCATGATTG TTTCAGTGAT 
GCCAGCCATG ATGTGGTGAG GGCGACCACT ATTGGAAGTT TCAAATTATG GCCAAGAAAG 
CTAGATCAAG AGTTTGGTGA GAAAGAGGTA ACAATTCTTA CAAAGCATTA TGAACCTGTG 
TTGGAAGGGG CCAATGTGAA CATTGATGAA GTGGACACAG AATGGAGCAT GATGAAATTA 
GAGATTTATA ATCGATTTCA GAACATCCGA AAGTTAACCT GGGATATTGT GAATTCCATC 
TACTCATACA AGTATCCCAA TATATTGACC CTAGTGGATC TGGTGCTGAC ACTCCCTGCA 
AGTTCAGCTG AGGCGGAACG TGGGTTCAGT CAGATGAAAC TAGCCAAGTC ACAAATGCAT 
TTTAAAACAG ATCCTGAGAA CATGACAGAC ATTTTAATAA TCCAGTTGAA TTCTCCAGAC 
ATCAATAAGT TTGATCCCAA GAAAGCCATC CATCTATGGA ATACTTCTTG GCAGAGAAAT 
ACACTTCCAC CAATTGACCA CACAATAACC AAGTCAGATT GCTCCTCTAG CTCAGATAGT 
CTTTATGAAA GTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016287093.1
CDS	84..6521
Translation	MSDILCRWLNQEVKLSRTVSSKGIEKDFSNGFLLGELLHKYELQDDFPLFSEGRVSNAKLNNFSRLEPTFQLLGVQFDQNVAQNIMTEKHGSVTILLYQLYIALQKKRKSGLTGVAMQTMRPLAPAKLQTMRSEIYRERLKNLVPRQTDLNLSRVSEKFEAKYKKMEEKIARVHFQNIRKFEKFQEEQRIQDLEKQRLGRRRQHEMMARIQAAILQIPKTVPNRTLKSIESHKMMMKKKEEEDVLNEIQKFEMLIKKELEAKGSGSKVPSAAQTTADLLSTYSDEEYIKKIQKRLEEDTLAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRVFREKQYEERRLKDFQDALDREAALARQAKLEYEEQAQKEIEIHERIAAERSQARYKKHYSMCTEILDNIIDLSTKIAEYRELTHNLIPVKIMRDWKELFFSGKPIYEIASIDSKPSRFTSEQLIELSKRNLLDDNDYEEYKNLTGEWVLPEDMADVPLPSNNNILGHVIRVVHEKVYPPKPAYIATQLPSSSIKGCMLGKTCSGKTSCLKILEEVFHIEILCMDTLVQEAIQAFLRNETVTEVETHKEDLSKEVQDIKDSNMTTIAEIIPTEEKKDENLPQQDEVIIKPPSAQDVRIRLTNRAQLGSKCEKLLKRGRSIPDVLLIQIMVNAINEISSDKGWILDGFPMTINQAKLLEEALTGFNTDQPEHEIKAHKKSTLVFDPSAPKDPPPPPPAFDFVMLLDITDTLVLNRVMGEEKRNTDTTGIPESATSEMFPEEQEKFELLRDQIQHRILGFLDNWPELEKWFSGKQDILITVNAEVDKRALATKLQELFIAELRKKEAKAKQELEEQEEERKKKEALPPEPPPPPPPEPEEEPKEAKGKGKGKGKGAKEGKGKKDTSPKGKDAAKEAKAKKSETSPKGRSPDKRSPEDKTSPPAPPGPPPVKAGSEDWVYVDEPLPQEIFEYLDTFWEVIESSYINTSKAIFRQIRDERHSVLYYLHNVRLNYQGYLKRPDHKQEFVSQWQEDFNNLPEDLWEDEETKAELHQRVNDLRDRLWDICDTRKEEAEQERADIIDESWLQDHLGILMNHFFSLMQVELSRFQDIKTFLQDYYRGMEGKIPTDLAKSGYTRVPLIDLDEEDTDITSKIPVIPRRQPSPEGLLNKPRSKLSLRGGRPDYCLENVQLKFERDEKTLMTIWQNTSSAINLLVNSEIQLRKLEDDKENKPTSTKEKSPQDGGKGPKGAKKKPDKKKVPSPVEAVSMVLTPEEQEALDKRNELKMKIKEEHITALQCEAQASIFRLELIKMKALSYMEELLGRAVEVYKLMDKWLGARYLAEMASVEKLTEVAHYHIETSTRIQNELILNEEDFYINGDVKVFPDPLPPVRPPPVEKEMNTFLTLEQLNTLRNEFLQMAPKAKTPWKYFKKGISSVDTRRHAEEKKRLRNLDYYLPKRNNQFLKIWLKKYPWLVYDDRSDLMFCGLCRKHGVSSGGNHVSFFYGTNNFRTEFLQAHDNSEAHTKAVLAEATGVSSLTDAAKEQILKNRNKVILGRIENLFRTCHAIAKTGRPLKDFVWMCKLDDMKGVDVGPIFRTDKSARTFIYFIAEVERICLREKLEKCKFFSLISDGGSGSFIEDTKVVYIQFAYEGRVCCQVVGVQTVDKIEPVAIKSAIEKTLEINLQLNLSSKDWSKKLVGFGSDGASVMVGENNGVARLLKEIQPCVQTVHCFAHRLELAYQEALQSIQLYSTMNDLLQNIYYFYHDSPSNTKALIAAFEDLKIRPVIPSRVGNNRWLPRLQMALQIFLKGYSPIVKQLRTMHEGRSSADQQKAQELLQFLLQAEVVRFAHFLLDVINVLSILSRVNQDRNSSIGDVFATLQSTLGTLKMYKKRPGPKERLTESATHFHGNQLTGNGNISEVRNTVLTNLSKHLHDCFSDASHDVVRATTIGSFKLWPRKLDQEFGEKEVTILTKHYEPVLEGANVNIDEVDTEWSMMKLEIYNRFQNIRKLTWDIVNSIYSYKYPNILTLVDLVLTLPASSAEAERGFSQMKLAKSQMHFKTDPENMTDILIIQLNSPDINKFDPKKAIHLWNTSWQRNTLPPIDHTITKSDCSSSSDSLYESD

Target ORF information:

RefSeq Version	XM_016431607.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016431607.1

ORF Insert Sequence:

ATGTCGGACA TCCTGTGCAG GTGGCTAAAC CAGGAGGTGA AGCTGTCCCG GACAGTGAGT 
TCAAAGGGGA TTGAAAAAGA TTTTTCTAAT GGCTTCCTAC TGGGTGAACT TCTGCACAAA 
TATGAACTTC AGGATGACTT TCCTCTGTTT TCAGAAGGCA GGGTTTCCAA TGCAAAACTT 
AATAATTTTT CTCGCTTAGA ACCAACATTT CAACTCCTGG GTGTTCAGTT CGATCAAAAT 
GTGGCCCAGA ATATTATGAC AGAAAAGCAT GGCTCTGTAA CCATACTTCT TTATCAGTTG 
TACATTGCGC TTCAAAAAAA GAGAAAAAGT GGACTCACGG GAGTAGCAAT GCAAACAATG 
AGACCTTTAG CACCTGCAAA GCTACAAACT ATGAGAAGTG AGATTTATCG AGAGAGACTT 
AAGAACCTGG TGCCTCGGCA AACTGATTTG AATCTAAGTC GGGTTTCAGA AAAGTTTGAA 
GCAAAGTATA AAAAAATGGA AGAAAAAATT GCTCGTGTTC ATTTTCAAAA TATCCGAAAA 
TTTGAAAAGT TCCAGGAAGA ACAAAGAATT CAAGATCTTG AAAAGCAGCG CCTGGGAAGA 
AGACGACAAC ATGAAATGAT GGCTAGAATC CAAGCAGCCA TTTTACAGAT TCCCAAAACA 
GTACCCAATC GTACTCTGAA ATCTATTGAG AGCCACAAAA TGATGATGAA GAAAAAAGAG 
GAAGAAGATG TGCTGAATGA AATTCAGAAG TTTGAAATGC TAATAAAAAA GGAGCTTGAG 
GCCAAAGGCA GTGGATCAAA AGTTCCTTCG GCAGCCCAGA CAACTGCTGA CTTGTTAAGC 
ACGTATTCAG ATGAGGAATA CATTAAAAAA ATTCAAAAAC GTCTAGAGGA AGATACCTTA 
GCCCGGGAAC AAAGGGAGAA AAGACGCAGA AAATTGTTAA TGGATCAATT GATAGCCCAT 
GAAGCTCAAG AGGAGGCTTA CCGAGAAGAA CAACTCATTA ACCGACTAAT GAGACAATCT 
CAGCAGGAAC GTCGGATTGC TGTGCAACTC ATGCATGTTC GACACGAAAA GGAAGTTTTA 
TGGCAAAACA GGGTTTTCAG AGAAAAACAA TATGAAGAAA GACGGCTGAA AGATTTCCAG 
GATGCTCTTG ACAGAGAAGC AGCTCTTGCA AGACAAGCCA AGCTGGAGTA TGAAGAACAA 
GCCCAAAAGG AAATAGAAAT TCATGAAAGA ATAGCAGCAG AAAGATCTCA AGCTCGTTAT 
AAAAAGCATT ATTCAATGTG CACAGAAATC TTGGACAATA TCATTGATTT ATCAACTAAA 
ATAGCAGAAT ATCGAGAATT AACACATAAT CTTATTCCAG TTAAAATTAT GCGTGATTGG 
AAGGAGCTGT TTTTCAGTGG AAAGCCAATT TATGAAATAG CCTCCATTGA CTCCAAGCCA 
TCTCGTTTTA CCTCAGAACA ACTAATTGAG CTATCCAAAA GGAATTTACT AGATGACAAT 
GATTATGAGG AGTACAAGAA CTTGACAGGA GAGTGGGTCC TCCCAGAAGA TATGGCTGAT 
GTTCCCCTAC CTTCCAACAA TAACATATTG GGTCATGTGA TTCGAGTAGT TCATGAAAAA 
GTTTACCCTC CAAAACCAGC ATATATTGCA ACTCAGTTAC CTTCATCTTC GATTAAAGGG 
TGCATGCTGG GCAAGACATG CAGTGGGAAA ACCAGCTGTC TAAAGATTTT AGAAGAAGTT 
TTCCATATTG AGATACTTTG TATGGATACT CTGGTTCAAG AGGCTATTCA GGCTTTCCTT 
AGAAATGAAA CTGTAACTGA GGTGGAAACA CATAAAGAAG ATTTATCAAA AGAAGTTCAA 
GATATTAAGG ATTCAAACAT GACGACTATT GCAGAAATAA TTCCTACTGA GGAGAAAAAA 
GATGAGAATC TACCACAACA AGATGAAGTG ATTATTAAAC CACCATCAGC CCAGGATGTG 
AGAATAAGAC TTACTAATCG TGCTCAACTT GGTTCAAAAT GTGAAAAGTT GCTCAAGAGA 
GGAAGAAGTA TCCCCGATGT ACTGCTAATT CAAATCATGG TGAATGCTAT TAATGAAATA 
TCATCAGACA AAGGATGGAT CTTGGATGGT TTTCCAATGA CCATAAATCA AGCAAAACTT 
CTTGAGGAAG CCCTTACAGG GTTCAACACA GATCAACCAG AACATGAAAT AAAGGCTCAC 
AAAAAATCAA CATTGGTTTT TGATCCATCT GCTCCCAAAG ACCCCCCTCC TCCCCCACCT 
GCATTTGATT TTGTTATGTT GCTGGATATA ACTGACACAT TGGTGCTGAA TCGTGTGATG 
GGAGAGGAGA AAAGAAACAC TGACACAACT GGTATACCAG AAAGTGCAAC TTCAGAAATG 
TTTCCTGAAG AACAAGAAAA GTTTGAACTT TTGAGGGATC AGATACAACA CAGAATTTTA 
GGTTTCTTGG ACAACTGGCC AGAGCTGGAA AAATGGTTTT CAGGAAAACA AGACATTCTA 
ATCACAGTCA ATGCAGAAGT AGATAAAAGG GCTTTAGCCA CAAAACTACA AGAGCTTTTT 
ATTGCTGAAC TGAGGAAAAA AGAAGCTAAA GCAAAACAGG AATTAGAAGA ACAGGAAGAG 
GAAAGGAAAA AGAAAGAAGC CCTCCCACCT GAACCGCCAC CTCCACCTCC CCCTGAACCA 
GAAGAAGAGC CAAAGGAAGC AAAAGGGAAA GGAAAAGGGA AAGGTAAAGG TGCTAAAGAA 
GGAAAAGGAA AGAAAGACAC TTCTCCCAAG GGCAAAGATG CTGCTAAAGA AGCAAAAGCA 
AAGAAAAGTG AAACTTCTCC AAAAGGAAGG TCACCAGACA AGAGGTCACC TGAAGATAAA 
ACATCACCTC CTGCACCTCC AGGACCACCA CCAGTCAAAG CAGGATCAGA AGACTGGGTC 
TATGTGGATG AGCCACTTCC TCAGGAAATA TTTGAATATC TAGACACTTT TTGGGAAGTA 
ATAGAAAGTT CATACATAAA TACCAGCAAA GCTATATTCA GACAGATAAG AGATGAGAGA 
CATAGTGTAC TTTATTATCT ACATAATGTC AGACTAAATT ACCAGGGATA TCTGAAACGG 
CCAGATCACA AGCAGGAATT TGTATCACAA TGGCAGGAGG ATTTTAACAA CCTCCCTGAA 
GACTTATGGG AGGATGAGGA AACAAAAGCT GAACTACACC AAAGAGTCAA TGATTTAAGG 
GACCGCCTGT GGGATATATG TGATACTCGA AAGGAAGAGG CAGAACAAGA ACGTGCTGAT 
ATCATTGATG AATCCTGGTT ACAAGATCAT CTTGGAATTC TTATGAATCA TTTCTTTTCT 
TTGATGCAGG TGGAACTTAG CCGTTTCCAG GACATAAAGA CTTTTCTACA AGATTACTAT 
AGGGGAATGG AAGGAAAAAT CCCCACCGAC CTTGCTAAAT CTGGATATAC TCGAGTTCCC 
CTTATAGATC TTGATGAAGA GGATACCGAT ATCACCTCCA AAATCCCTGT AATTCCTCGG 
AGACAGCCTT CTCCTGAAGG ACTATTAAAC AAGCCCAGAA GTAAACTATC CCTGAGAGGA 
GGCAGACCTG ATTACTGCTT AGAAAATGTC CAACTCAAAT TTGAAAGAGA TGAGAAGACC 
CTTATGACTA TATGGCAGAA TACATCTTCA GCCATCAACC TCTTGGTAAA TTCTGAAATA 
CAGTTGAGGA AACTAGAAGA TGATAAAGAA AACAAACCAA CATCAACTAA AGAAAAATCT 
CCTCAAGATG GTGGCAAAGG TCCTAAAGGT GCTAAGAAAA AGCCAGATAA GAAGAAAGTT 
CCATCACCAG TTGAAGCCGT TTCTATGGTT CTAACACCAG AAGAACAGGA GGCTCTTGAT 
AAGAGGAATG AATTGAAAAT GAAAATAAAG GAAGAACATA TTACAGCTCT TCAGTGTGAA 
GCACAAGCTT CAATATTCCG ACTGGAACTG ATCAAGATGA AGGCCTTATC TTACATGGAA 
GAGCTGCTAG GAAGAGCAGT TGAGGTTTAC AAACTGATGG ACAAATGGCT CGGAGCCCGA 
TACTTAGCTG AAATGGCAAG TGTGGAAAAA TTAACTGAAG TGGCTCACTA CCACATTGAA 
ACCTCAACAA GGATTCAGAA TGAACTTATT TTAAATGAAG AAGATTTCTA CATCAATGGT 
GATGTGAAAG TCTTTCCAGA TCCGCTTCCT CCCGTCCGGC CTCCACCTGT GGAGAAAGAA 
ATGAATACTT TTCTCACTCT TGAACAACTC AACACCCTTC GAAATGAGTT TCTCCAGATG 
GCACCAAAAG CCAAAACACC ATGGAAGTAT TTTAAAAAAG GAATCTCTTC AGTTGATACT 
AGAAGACATG CTGAGGAAAA GAAAAGGCTA AGAAATCTTG ACTATTACCT ACCTAAGAGG 
AACAACCAAT TTTTGAAAAT TTGGCTAAAA AAATACCCTT GGCTCGTGTA TGATGACAGG 
TCAGACTTAA TGTTCTGTGG CTTGTGTCGC AAACATGGAG TGAGCTCAGG AGGAAACCAT 
GTTAGCTTTT TTTATGGCAC CAACAACTTT AGGACTGAAT TTCTCCAAGC ACATGACAAT 
AGTGAGGCAC ACACGAAGGC TGTTCTAGCA GAAGCAACAG GGGTTTCTTC CCTGACTGAT 
GCTGCCAAGG AGCAGATTCT AAAGAACAGG AACAAAGTAA TCTTGGGAAG AATAGAAAAC 
CTCTTTAGAA CATGTCATGC TATTGCCAAG ACTGGACGTC CCCTCAAAGA CTTTGTCTGG 
ATGTGTAAAC TGGATGATAT GAAGGGTGTA GACGTTGGCC CAATATTCAG GACTGACAAG 
TCAGCCAGAA CTTTCATATA CTTTATTGCT GAAGTAGAAC GCATATGTCT TCGAGAGAAA 
TTAGAAAAAT GTAAGTTCTT CTCACTCATC AGTGATGGTG GCTCAGGCAG CTTCATAGAA 
GACACCAAAG TTGTTTACAT CCAGTTTGCA TATGAGGGCA GGGTCTGCTG TCAGGTTGTT 
GGAGTACAGA CTGTGGACAA AATTGAACCG GTGGCAATAA AAAGTGCCAT TGAAAAAACA 
CTTGAGATAA ATCTTCAACT AAATCTGTCT AGCAAAGACT GGTCCAAAAA ACTCGTTGGG 
TTTGGAAGTG ATGGTGCATC TGTCATGGTG GGAGAGAACA ATGGTGTAGC CAGGCTTCTG 
AAAGAAATTC AGCCATGTGT GCAAACAGTC CACTGCTTTG CACATAGACT TGAACTAGCC 
TACCAAGAAG CATTGCAGAG CATCCAATTA TACAGTACTA TGAATGATCT TCTCCAGAAT 
ATATATTATT TTTATCATGA TTCACCTTCA AATACAAAAG CTCTGATAGC TGCCTTTGAA 
GACCTTAAAA TCCGCCCTGT GATTCCCTCT CGAGTTGGCA ACAACAGATG GCTCCCTCGG 
TTACAGATGG CTCTACAAAT CTTTCTCAAA GGATATTCTC CAATTGTCAA GCAATTAAGA 
ACAATGCATG AAGGCAGAAG TTCCGCTGAT CAACAGAAAG CTCAAGAGCT CCTACAGTTC 
CTTCTCCAAG CTGAGGTTGT CAGATTTGCT CACTTCTTGC TGGATGTAAT CAACGTCCTC 
AGCATTTTGT CTCGAGTCAA TCAGGACAGA AATTCATCAA TAGGAGATGT ATTTGCAACC 
CTACAATCAA CACTAGGAAC ACTCAAGATG TATAAAAAAA GACCAGGTCC AAAGGAACGT 
CTGACAGAGT CAGCCACACA CTTTCATGGT AACCAACTGA CAGGAAATGG AAATATCTCT 
GAAGTACGAA ACACAGTCTT AACAAATCTC TCAAAGCATC TTCATGATTG TTTCAGTGAT 
GCCAGCCATG ATGTGGTGAG GGCGACCACT ATTGGAAGTT TCAAATTATG GCCAAGAAAG 
CTAGATCAAG AGTTTGGTGA GAAAGAGGTA ACAATTCTTA CAAAGCATTA TGAACCTGTG 
TTGGAAGGGG CCAATGTGAA CATTGATGAA GTGGACACAG AATGGAGCAT GATGAAATTA 
GAGATTTATA ATCGATTTCA GAACATCCGA AAGTTAACCT GGGATATTGT GAATTCCATC 
TACTCATACA AGTATCCCAA TATATTGACC CTAGTGGATC TGGTGCTGAC ACTCCCTGCA 
AGTTCAGCTG AGGCGGAACG TGGGTTCAGT CAGATGAAAC TAGCCAAGTC ACAAATGCAT 
TTTAAAACAG ATCCTGAGAA CATGACAGAC ATTTTAATAA TCCAGTTGAA TTCTCCAGAC 
ATCAATAAGT TTGATCCCAA GAAAGCCATC CATCTATGGA ATACTTCTTG GCAGAGAAAT 
ACACTTCCAC CAATTGACCA CACAATAACC AAGTCAGATT GCTCCTCTAG CTCAGATAGT 
CTTTATGAAA GTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_016431608.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016287094.1 Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.

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CloneID	OMc41043
Clone ID Related Accession (Same CDS sequence)	XM_016431608.1
Accession Version	XM_016431608.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	sperm flagellar protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008803.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACA TCCTGTGCAG GTGGCTAAAC CAGGAGGTGA AGCTGTCCCG GACAGTGAGT 
TCAAAGGGGA TTGAAAAAGA TTTTTCTAAT GGCTTCCTAC TGGGTGAACT TCTGCACAAA 
TATGAACTTC AGGATGACTT TCCTCTGTTT TCAGAAGGCA GGGTTTCCAA TGCAAAACTT 
AATAATTTTT CTCGCTTAGA ACCAACATTT CAACTCCTGG GTGTTCAGTT CGATCAAAAT 
GTGGCCCAGA ATATTATGAC AGAAAAGCAT GGCTCTGTAA CCATACTTCT TTATCAGTTG 
TACATTGCGC TTCAAAAAAA GAGAAAAAGT GGACTCACGG GAGTAGCAAT GCAAACAATG 
AGACCTTTAG CACCTGCAAA GCTACAAACT ATGAGAAGTG AGATTTATCG AGAGCAGCGC 
CTGGGAAGAA GACGACAACA TGAAATGATG GCTAGAATCC AAGCAGCCAT TTTACAGATT 
CCCAAAACAG TACCCAATCG TACTCTGAAA TCTATTGAGA GCCACAAAAT GATGATGAAG 
AAAAAAGAGG AAGAAGATGT GCTGAATGAA ATTCAGAAGT TTGAAATGCT AATAAAAAAG 
GAGCTTGAGG CCAAAGGCAG TGGATCAAAA GTTCCTTCGG CAGCCCAGAC AACTGCTGAC 
TTGTTAAGCA CGTATTCAGA TGAGGAATAC ATTAAAAAAA TTCAAAAACG TCTAGAGGAA 
GATACCTTAG CCCGGGAACA AAGGGAGAAA AGACGCAGAA AATTGTTAAT GGATCAATTG 
ATAGCCCATG AAGCTCAAGA GGAGGCTTAC CGAGAAGAAC AACTCATTAA CCGACTAATG 
AGACAATCTC AGCAGGAACG TCGGATTGCT GTGCAACTCA TGCATGTTCG ACACGAAAAG 
GAAGTTTTAT GGCAAAACAG GGTTTTCAGA GAAAAACAAT ATGAAGAAAG ACGGCTGAAA 
GATTTCCAGG ATGCTCTTGA CAGAGAAGCA GCTCTTGCAA GACAAGCCAA GCTGGAGTAT 
GAAGAACAAG CCCAAAAGGA AATAGAAATT CATGAAAGAA TAGCAGCAGA AAGATCTCAA 
GCTCGTTATA AAAAGCATTA TTCAATGTGC ACAGAAATCT TGGACAATAT CATTGATTTA 
TCAACTAAAA TAGCAGAATA TCGAGAATTA ACACATAATC TTATTCCAGT TAAAATTATG 
CGTGATTGGA AGGAGCTGTT TTTCAGTGGA AAGCCAATTT ATGAAATAGC CTCCATTGAC 
TCCAAGCCAT CTCGTTTTAC CTCAGAACAA CTAATTGAGC TATCCAAAAG GAATTTACTA 
GATGACAATG ATTATGAGGA GTACAAGAAC TTGACAGGAG AGTGGGTCCT CCCAGAAGAT 
ATGGCTGATG TTCCCCTACC TTCCAACAAT AACATATTGG GTCATGTGAT TCGAGTAGTT 
CATGAAAAAG TTTACCCTCC AAAACCAGCA TATATTGCAA CTCAGTTACC TTCATCTTCG 
ATTAAAGGGT GCATGCTGGG CAAGACATGC AGTGGGAAAA CCAGCTGTCT AAAGATTTTA 
GAAGAAGTTT TCCATATTGA GATACTTTGT ATGGATACTC TGGTTCAAGA GGCTATTCAG 
GCTTTCCTTA GAAATGAAAC TGTAACTGAG GTGGAAACAC ATAAAGAAGA TTTATCAAAA 
GAAGTTCAAG ATATTAAGGA TTCAAACATG ACGACTATTG CAGAAATAAT TCCTACTGAG 
GAGAAAAAAG ATGAGAATCT ACCACAACAA GATGAAGTGA TTATTAAACC ACCATCAGCC 
CAGGATGTGA GAATAAGACT TACTAATCGT GCTCAACTTG GTTCAAAATG TGAAAAGTTG 
CTCAAGAGAG GAAGAAGTAT CCCCGATGTA CTGCTAATTC AAATCATGGT GAATGCTATT 
AATGAAATAT CATCAGACAA AGGATGGATC TTGGATGGTT TTCCAATGAC CATAAATCAA 
GCAAAACTTC TTGAGGAAGC CCTTACAGGG TTCAACACAG ATCAACCAGA ACATGAAATA 
AAGGCTCACA AAAAATCAAC ATTGGTTTTT GATCCATCTG CTCCCAAAGA CCCCCCTCCT 
CCCCCACCTG CATTTGATTT TGTTATGTTG CTGGATATAA CTGACACATT GGTGCTGAAT 
CGTGTGATGG GAGAGGAGAA AAGAAACACT GACACAACTG GTATACCAGA AAGTGCAACT 
TCAGAAATGT TTCCTGAAGA ACAAGAAAAG TTTGAACTTT TGAGGGATCA GATACAACAC 
AGAATTTTAG GTTTCTTGGA CAACTGGCCA GAGCTGGAAA AATGGTTTTC AGGAAAACAA 
GACATTCTAA TCACAGTCAA TGCAGAAGTA GATAAAAGGG CTTTAGCCAC AAAACTACAA 
GAGCTTTTTA TTGCTGAACT GAGGAAAAAA GAAGCTAAAG CAAAACAGGA ATTAGAAGAA 
CAGGAAGAGG AAAGGAAAAA GAAAGAAGCC CTCCCACCTG AACCGCCACC TCCACCTCCC 
CCTGAACCAG AAGAAGAGCC AAAGGAAGCA AAAGGGAAAG GAAAAGGGAA AGGTAAAGGT 
GCTAAAGAAG GAAAAGGAAA GAAAGACACT TCTCCCAAGG GCAAAGATGC TGCTAAAGAA 
GCAAAAGCAA AGAAAAGTGA AACTTCTCCA AAAGGCAAAG GAAGGTCACC AGACAAGAGG 
TCACCTGAAG ATAAAACATC ACCTCCTGCA CCTCCAGGAC CACCACCAGT CAAAGCAGGA 
TCAGAAGACT GGGTCTATGT GGATGAGCCA CTTCCTCAGG AAATATTTGA ATATCTAGAC 
ACTTTTTGGG AAGTAATAGA AAGTTCATAC ATAAATACCA GCAAAGCTAT ATTCAGACAG 
ATAAGAGATG AGAGACATAG TGTACTTTAT TATCTACATA ATGTCAGACT AAATTACCAG 
GGATATCTGA AACGGCCAGA TCACAAGCAG GAATTTGTAT CACAATGGCA GGAGGATTTT 
AACAACCTCC CTGAAGACTT ATGGGAGGAT GAGGAAACAA AAGCTGAACT ACACCAAAGA 
GTCAATGATT TAAGGGACCG CCTGTGGGAT ATATGTGATA CTCGAAAGGA AGAGGCAGAA 
CAAGAACGTG CTGATATCAT TGATGAATCC TGGTTACAAG ATCATCTTGG AATTCTTATG 
AATCATTTCT TTTCTTTGAT GCAGGTGGAA CTTAGCCGTT TCCAGGACAT AAAGACTTTT 
CTACAAGATT ACTATAGGGG AATGGAAGGA AAAATCCCCA CCGACCTTGC TAAATCTGGA 
TATACTCGAG TTCCCCTTAT AGATCTTGAT GAAGAGGATA CCGATATCAC CTCCAAAATC 
CCTGTAATTC CTCGGAGACA GCCTTCTCCT GAAGGACTAT TAAACAAGCC CAGAAGTAAA 
CTATCCCTGA GAGGAGGCAG ACCTGATTAC TGCTTAGAAA ATGTCCAACT CAAATTTGAA 
AGAGATGAGA AGACCCTTAT GACTATATGG CAGAATACAT CTTCAGCCAT CAACCTCTTG 
GTAAATTCTG AAATACAGTT GAGGAAACTA GAAGATGATA AAGAAAACAA ACCAACATCA 
ACTAAAGAAA AATCTCCTCA AGATGGTGGC AAAGGTCCTA AAGGTGCTAA GAAAAAGCCA 
GATAAGAAGA AAGTTCCATC ACCAGTTGAA GCCGTTTCTA TGGTTCTAAC ACCAGAAGAA 
CAGGAGGCTC TTGATAAGAG GAATGAATTG AAAATGAAAA TAAAGGAAGA ACATATTACA 
GCTCTTCAGT GTGAAGCACA AGCTTCAATA TTCCGACTGG AACTGATCAA GATGAAGGCC 
TTATCTTACA TGGAAGAGCT GCTAGGAAGA GCAGTTGAGG TTTACAAACT GATGGACAAA 
TGGCTCGGAG CCCGATACTT AGCTGAAATG GCAAGTGTGG AAAAATTAAC TGAAGTGGCT 
CACTACCACA TTGAAACCTC AACAAGGATT CAGAATGAAC TTATTTTAAA TGAAGAAGAT 
TTCTACATCA ATGGTGATGT GAAAGTCTTT CCAGATCCGC TTCCTCCCGT CCGGCCTCCA 
CCTGTGGAGA AAGAAATGAA TACTTTTCTC ACTCTTGAAC AACTCAACAC CCTTCGAAAT 
GAGTTTCTCC AGATGGCACC AAAAGCCAAA ACACCATGGA AGTATTTTAA AAAAGGAATC 
TCTTCAGTTG ATACTAGAAG ACATGCTGAG GAAAAGAAAA GGCTAAGAAA TCTTGACTAT 
TACCTACCTA AGAGGAACAA CCAATTTTTG AAAATTTGGC TAAAAAAATA CCCTTGGCTC 
GTGTATGATG ACAGGTCAGA CTTAATGTTC TGTGGCTTGT GTCGCAAACA TGGAGTGAGC 
TCAGGAGGAA ACCATGTTAG CTTTTTTTAT GGCACCAACA ACTTTAGGAC TGAATTTCTC 
CAAGCACATG ACAATAGTGA GGCACACACG AAGGCTGTTC TAGCAGAAGC AACAGGGGTT 
TCTTCCCTGA CTGATGCTGC CAAGGAGCAG ATTCTAAAGA ACAGGAACAA AGTAATCTTG 
GGAAGAATAG AAAACCTCTT TAGAACATGT CATGCTATTG CCAAGACTGG ACGTCCCCTC 
AAAGACTTTG TCTGGATGTG TAAACTGGAT GATATGAAGG GTGTAGACGT TGGCCCAATA 
TTCAGGACTG ACAAGTCAGC CAGAACTTTC ATATACTTTA TTGCTGAAGT AGAACGCATA 
TGTCTTCGAG AGAAATTAGA AAAATGTAAG TTCTTCTCAC TCATCAGTGA TGGTGGCTCA 
GGCAGCTTCA TAGAAGACAC CAAAGTTGTT TACATCCAGT TTGCATATGA GGGCAGGGTC 
TGCTGTCAGG TTGTTGGAGT ACAGACTGTG GACAAAATTG AACCGGTGGC AATAAAAAGT 
GCCATTGAAA AAACACTTGA GATAAATCTT CAACTAAATC TGTCTAGCAA AGACTGGTCC 
AAAAAACTCG TTGGGTTTGG AAGTGATGGT GCATCTGTCA TGGTGGGAGA GAACAATGGT 
GTAGCCAGGC TTCTGAAAGA AATTCAGCCA TGTGTGCAAA CAGTCCACTG CTTTGCACAT 
AGACTTGAAC TAGCCTACCA AGAAGCATTG CAGAGCATCC AATTATACAG TACTATGAAT 
GATCTTCTCC AGAATATATA TTATTTTTAT CATGATTCAC CTTCAAATAC AAAAGCTCTG 
ATAGCTGCCT TTGAAGACCT TAAAATCCGC CCTGTGATTC CCTCTCGAGT TGGCAACAAC 
AGATGGCTCC CTCGGTTACA GATGGCTCTA CAAATCTTTC TCAAAGGATA TTCTCCAATT 
GTCAAGCAAT TAAGAACAAT GCATGAAGGC AGAAGTTCCG CTGATCAACA GAAAGCTCAA 
GAGCTCCTAC AGTTCCTTCT CCAAGCTGAG GTTGTCAGAT TTGCTCACTT CTTGCTGGAT 
GTAATCAACG TCCTCAGCAT TTTGTCTCGA GTCAATCAGG ACAGAAATTC ATCAATAGGA 
GATGTATTTG CAACCCTACA ATCAACACTA GGAACACTCA AGATGTATAA AAAAAGACCA 
GGTCCAAAGG AACGTCTGAC AGAGTCAGCC ACACACTTTC ATGGTAACCA ACTGACAGGA 
AATGGAAATA TCTCTGAAGT ACGAAACACA GTCTTAACAA ATCTCTCAAA GCATCTTCAT 
GATTGTTTCA GTGATGCCAG CCATGATGTG GTGAGGGCGA CCACTATTGG AAGTTTCAAA 
TTATGGCCAA GAAAGCTAGA TCAAGAGTTT GGTGAGAAAG AGGTAACAAT TCTTACAAAG 
CATTATGAAC CTGTGTTGGA AGGGGCCAAT GTGAACATTG ATGAAGTGGA CACAGAATGG 
AGCATGATGA AATTAGAGAT TTATAATCGA TTTCAGAACA TCCGAAAGTT AACCTGGGAT 
ATTGTGAATT CCATCTACTC ATACAAGTAT CCCAATATAT TGACCCTAGT GGATCTGGTG 
CTGACACTCC CTGCAAGTTC AGCTGAGGCG GAACGTGGGT TCAGTCAGAT GAAACTAGCC 
AAGTCACAAA TGCATTTTAA AACAGATCCT GAGAACATGA CAGACATTTT AATAATCCAG 
TTGAATTCTC CAGACATCAA TAAGTTTGAT CCCAAGAAAG CCATCCATCT ATGGAATACT 
TCTTGGCAGA GAAATACACT TCCACCAATT GACCACACAA TAACCAAGTC AGATTGCTCC 
TCTAGCTCAG ATAGTCTTTA TGAAAGTGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016287094.1
CDS	86..6358
Translation	MSDILCRWLNQEVKLSRTVSSKGIEKDFSNGFLLGELLHKYELQDDFPLFSEGRVSNAKLNNFSRLEPTFQLLGVQFDQNVAQNIMTEKHGSVTILLYQLYIALQKKRKSGLTGVAMQTMRPLAPAKLQTMRSEIYREQRLGRRRQHEMMARIQAAILQIPKTVPNRTLKSIESHKMMMKKKEEEDVLNEIQKFEMLIKKELEAKGSGSKVPSAAQTTADLLSTYSDEEYIKKIQKRLEEDTLAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRVFREKQYEERRLKDFQDALDREAALARQAKLEYEEQAQKEIEIHERIAAERSQARYKKHYSMCTEILDNIIDLSTKIAEYRELTHNLIPVKIMRDWKELFFSGKPIYEIASIDSKPSRFTSEQLIELSKRNLLDDNDYEEYKNLTGEWVLPEDMADVPLPSNNNILGHVIRVVHEKVYPPKPAYIATQLPSSSIKGCMLGKTCSGKTSCLKILEEVFHIEILCMDTLVQEAIQAFLRNETVTEVETHKEDLSKEVQDIKDSNMTTIAEIIPTEEKKDENLPQQDEVIIKPPSAQDVRIRLTNRAQLGSKCEKLLKRGRSIPDVLLIQIMVNAINEISSDKGWILDGFPMTINQAKLLEEALTGFNTDQPEHEIKAHKKSTLVFDPSAPKDPPPPPPAFDFVMLLDITDTLVLNRVMGEEKRNTDTTGIPESATSEMFPEEQEKFELLRDQIQHRILGFLDNWPELEKWFSGKQDILITVNAEVDKRALATKLQELFIAELRKKEAKAKQELEEQEEERKKKEALPPEPPPPPPPEPEEEPKEAKGKGKGKGKGAKEGKGKKDTSPKGKDAAKEAKAKKSETSPKGKGRSPDKRSPEDKTSPPAPPGPPPVKAGSEDWVYVDEPLPQEIFEYLDTFWEVIESSYINTSKAIFRQIRDERHSVLYYLHNVRLNYQGYLKRPDHKQEFVSQWQEDFNNLPEDLWEDEETKAELHQRVNDLRDRLWDICDTRKEEAEQERADIIDESWLQDHLGILMNHFFSLMQVELSRFQDIKTFLQDYYRGMEGKIPTDLAKSGYTRVPLIDLDEEDTDITSKIPVIPRRQPSPEGLLNKPRSKLSLRGGRPDYCLENVQLKFERDEKTLMTIWQNTSSAINLLVNSEIQLRKLEDDKENKPTSTKEKSPQDGGKGPKGAKKKPDKKKVPSPVEAVSMVLTPEEQEALDKRNELKMKIKEEHITALQCEAQASIFRLELIKMKALSYMEELLGRAVEVYKLMDKWLGARYLAEMASVEKLTEVAHYHIETSTRIQNELILNEEDFYINGDVKVFPDPLPPVRPPPVEKEMNTFLTLEQLNTLRNEFLQMAPKAKTPWKYFKKGISSVDTRRHAEEKKRLRNLDYYLPKRNNQFLKIWLKKYPWLVYDDRSDLMFCGLCRKHGVSSGGNHVSFFYGTNNFRTEFLQAHDNSEAHTKAVLAEATGVSSLTDAAKEQILKNRNKVILGRIENLFRTCHAIAKTGRPLKDFVWMCKLDDMKGVDVGPIFRTDKSARTFIYFIAEVERICLREKLEKCKFFSLISDGGSGSFIEDTKVVYIQFAYEGRVCCQVVGVQTVDKIEPVAIKSAIEKTLEINLQLNLSSKDWSKKLVGFGSDGASVMVGENNGVARLLKEIQPCVQTVHCFAHRLELAYQEALQSIQLYSTMNDLLQNIYYFYHDSPSNTKALIAAFEDLKIRPVIPSRVGNNRWLPRLQMALQIFLKGYSPIVKQLRTMHEGRSSADQQKAQELLQFLLQAEVVRFAHFLLDVINVLSILSRVNQDRNSSIGDVFATLQSTLGTLKMYKKRPGPKERLTESATHFHGNQLTGNGNISEVRNTVLTNLSKHLHDCFSDASHDVVRATTIGSFKLWPRKLDQEFGEKEVTILTKHYEPVLEGANVNIDEVDTEWSMMKLEIYNRFQNIRKLTWDIVNSIYSYKYPNILTLVDLVLTLPASSAEAERGFSQMKLAKSQMHFKTDPENMTDILIIQLNSPDINKFDPKKAIHLWNTSWQRNTLPPIDHTITKSDCSSSSDSLYESD

Target ORF information:

RefSeq Version	XM_016431608.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016431608.1

ORF Insert Sequence:

ATGTCGGACA TCCTGTGCAG GTGGCTAAAC CAGGAGGTGA AGCTGTCCCG GACAGTGAGT 
TCAAAGGGGA TTGAAAAAGA TTTTTCTAAT GGCTTCCTAC TGGGTGAACT TCTGCACAAA 
TATGAACTTC AGGATGACTT TCCTCTGTTT TCAGAAGGCA GGGTTTCCAA TGCAAAACTT 
AATAATTTTT CTCGCTTAGA ACCAACATTT CAACTCCTGG GTGTTCAGTT CGATCAAAAT 
GTGGCCCAGA ATATTATGAC AGAAAAGCAT GGCTCTGTAA CCATACTTCT TTATCAGTTG 
TACATTGCGC TTCAAAAAAA GAGAAAAAGT GGACTCACGG GAGTAGCAAT GCAAACAATG 
AGACCTTTAG CACCTGCAAA GCTACAAACT ATGAGAAGTG AGATTTATCG AGAGCAGCGC 
CTGGGAAGAA GACGACAACA TGAAATGATG GCTAGAATCC AAGCAGCCAT TTTACAGATT 
CCCAAAACAG TACCCAATCG TACTCTGAAA TCTATTGAGA GCCACAAAAT GATGATGAAG 
AAAAAAGAGG AAGAAGATGT GCTGAATGAA ATTCAGAAGT TTGAAATGCT AATAAAAAAG 
GAGCTTGAGG CCAAAGGCAG TGGATCAAAA GTTCCTTCGG CAGCCCAGAC AACTGCTGAC 
TTGTTAAGCA CGTATTCAGA TGAGGAATAC ATTAAAAAAA TTCAAAAACG TCTAGAGGAA 
GATACCTTAG CCCGGGAACA AAGGGAGAAA AGACGCAGAA AATTGTTAAT GGATCAATTG 
ATAGCCCATG AAGCTCAAGA GGAGGCTTAC CGAGAAGAAC AACTCATTAA CCGACTAATG 
AGACAATCTC AGCAGGAACG TCGGATTGCT GTGCAACTCA TGCATGTTCG ACACGAAAAG 
GAAGTTTTAT GGCAAAACAG GGTTTTCAGA GAAAAACAAT ATGAAGAAAG ACGGCTGAAA 
GATTTCCAGG ATGCTCTTGA CAGAGAAGCA GCTCTTGCAA GACAAGCCAA GCTGGAGTAT 
GAAGAACAAG CCCAAAAGGA AATAGAAATT CATGAAAGAA TAGCAGCAGA AAGATCTCAA 
GCTCGTTATA AAAAGCATTA TTCAATGTGC ACAGAAATCT TGGACAATAT CATTGATTTA 
TCAACTAAAA TAGCAGAATA TCGAGAATTA ACACATAATC TTATTCCAGT TAAAATTATG 
CGTGATTGGA AGGAGCTGTT TTTCAGTGGA AAGCCAATTT ATGAAATAGC CTCCATTGAC 
TCCAAGCCAT CTCGTTTTAC CTCAGAACAA CTAATTGAGC TATCCAAAAG GAATTTACTA 
GATGACAATG ATTATGAGGA GTACAAGAAC TTGACAGGAG AGTGGGTCCT CCCAGAAGAT 
ATGGCTGATG TTCCCCTACC TTCCAACAAT AACATATTGG GTCATGTGAT TCGAGTAGTT 
CATGAAAAAG TTTACCCTCC AAAACCAGCA TATATTGCAA CTCAGTTACC TTCATCTTCG 
ATTAAAGGGT GCATGCTGGG CAAGACATGC AGTGGGAAAA CCAGCTGTCT AAAGATTTTA 
GAAGAAGTTT TCCATATTGA GATACTTTGT ATGGATACTC TGGTTCAAGA GGCTATTCAG 
GCTTTCCTTA GAAATGAAAC TGTAACTGAG GTGGAAACAC ATAAAGAAGA TTTATCAAAA 
GAAGTTCAAG ATATTAAGGA TTCAAACATG ACGACTATTG CAGAAATAAT TCCTACTGAG 
GAGAAAAAAG ATGAGAATCT ACCACAACAA GATGAAGTGA TTATTAAACC ACCATCAGCC 
CAGGATGTGA GAATAAGACT TACTAATCGT GCTCAACTTG GTTCAAAATG TGAAAAGTTG 
CTCAAGAGAG GAAGAAGTAT CCCCGATGTA CTGCTAATTC AAATCATGGT GAATGCTATT 
AATGAAATAT CATCAGACAA AGGATGGATC TTGGATGGTT TTCCAATGAC CATAAATCAA 
GCAAAACTTC TTGAGGAAGC CCTTACAGGG TTCAACACAG ATCAACCAGA ACATGAAATA 
AAGGCTCACA AAAAATCAAC ATTGGTTTTT GATCCATCTG CTCCCAAAGA CCCCCCTCCT 
CCCCCACCTG CATTTGATTT TGTTATGTTG CTGGATATAA CTGACACATT GGTGCTGAAT 
CGTGTGATGG GAGAGGAGAA AAGAAACACT GACACAACTG GTATACCAGA AAGTGCAACT 
TCAGAAATGT TTCCTGAAGA ACAAGAAAAG TTTGAACTTT TGAGGGATCA GATACAACAC 
AGAATTTTAG GTTTCTTGGA CAACTGGCCA GAGCTGGAAA AATGGTTTTC AGGAAAACAA 
GACATTCTAA TCACAGTCAA TGCAGAAGTA GATAAAAGGG CTTTAGCCAC AAAACTACAA 
GAGCTTTTTA TTGCTGAACT GAGGAAAAAA GAAGCTAAAG CAAAACAGGA ATTAGAAGAA 
CAGGAAGAGG AAAGGAAAAA GAAAGAAGCC CTCCCACCTG AACCGCCACC TCCACCTCCC 
CCTGAACCAG AAGAAGAGCC AAAGGAAGCA AAAGGGAAAG GAAAAGGGAA AGGTAAAGGT 
GCTAAAGAAG GAAAAGGAAA GAAAGACACT TCTCCCAAGG GCAAAGATGC TGCTAAAGAA 
GCAAAAGCAA AGAAAAGTGA AACTTCTCCA AAAGGCAAAG GAAGGTCACC AGACAAGAGG 
TCACCTGAAG ATAAAACATC ACCTCCTGCA CCTCCAGGAC CACCACCAGT CAAAGCAGGA 
TCAGAAGACT GGGTCTATGT GGATGAGCCA CTTCCTCAGG AAATATTTGA ATATCTAGAC 
ACTTTTTGGG AAGTAATAGA AAGTTCATAC ATAAATACCA GCAAAGCTAT ATTCAGACAG 
ATAAGAGATG AGAGACATAG TGTACTTTAT TATCTACATA ATGTCAGACT AAATTACCAG 
GGATATCTGA AACGGCCAGA TCACAAGCAG GAATTTGTAT CACAATGGCA GGAGGATTTT 
AACAACCTCC CTGAAGACTT ATGGGAGGAT GAGGAAACAA AAGCTGAACT ACACCAAAGA 
GTCAATGATT TAAGGGACCG CCTGTGGGAT ATATGTGATA CTCGAAAGGA AGAGGCAGAA 
CAAGAACGTG CTGATATCAT TGATGAATCC TGGTTACAAG ATCATCTTGG AATTCTTATG 
AATCATTTCT TTTCTTTGAT GCAGGTGGAA CTTAGCCGTT TCCAGGACAT AAAGACTTTT 
CTACAAGATT ACTATAGGGG AATGGAAGGA AAAATCCCCA CCGACCTTGC TAAATCTGGA 
TATACTCGAG TTCCCCTTAT AGATCTTGAT GAAGAGGATA CCGATATCAC CTCCAAAATC 
CCTGTAATTC CTCGGAGACA GCCTTCTCCT GAAGGACTAT TAAACAAGCC CAGAAGTAAA 
CTATCCCTGA GAGGAGGCAG ACCTGATTAC TGCTTAGAAA ATGTCCAACT CAAATTTGAA 
AGAGATGAGA AGACCCTTAT GACTATATGG CAGAATACAT CTTCAGCCAT CAACCTCTTG 
GTAAATTCTG AAATACAGTT GAGGAAACTA GAAGATGATA AAGAAAACAA ACCAACATCA 
ACTAAAGAAA AATCTCCTCA AGATGGTGGC AAAGGTCCTA AAGGTGCTAA GAAAAAGCCA 
GATAAGAAGA AAGTTCCATC ACCAGTTGAA GCCGTTTCTA TGGTTCTAAC ACCAGAAGAA 
CAGGAGGCTC TTGATAAGAG GAATGAATTG AAAATGAAAA TAAAGGAAGA ACATATTACA 
GCTCTTCAGT GTGAAGCACA AGCTTCAATA TTCCGACTGG AACTGATCAA GATGAAGGCC 
TTATCTTACA TGGAAGAGCT GCTAGGAAGA GCAGTTGAGG TTTACAAACT GATGGACAAA 
TGGCTCGGAG CCCGATACTT AGCTGAAATG GCAAGTGTGG AAAAATTAAC TGAAGTGGCT 
CACTACCACA TTGAAACCTC AACAAGGATT CAGAATGAAC TTATTTTAAA TGAAGAAGAT 
TTCTACATCA ATGGTGATGT GAAAGTCTTT CCAGATCCGC TTCCTCCCGT CCGGCCTCCA 
CCTGTGGAGA AAGAAATGAA TACTTTTCTC ACTCTTGAAC AACTCAACAC CCTTCGAAAT 
GAGTTTCTCC AGATGGCACC AAAAGCCAAA ACACCATGGA AGTATTTTAA AAAAGGAATC 
TCTTCAGTTG ATACTAGAAG ACATGCTGAG GAAAAGAAAA GGCTAAGAAA TCTTGACTAT 
TACCTACCTA AGAGGAACAA CCAATTTTTG AAAATTTGGC TAAAAAAATA CCCTTGGCTC 
GTGTATGATG ACAGGTCAGA CTTAATGTTC TGTGGCTTGT GTCGCAAACA TGGAGTGAGC 
TCAGGAGGAA ACCATGTTAG CTTTTTTTAT GGCACCAACA ACTTTAGGAC TGAATTTCTC 
CAAGCACATG ACAATAGTGA GGCACACACG AAGGCTGTTC TAGCAGAAGC AACAGGGGTT 
TCTTCCCTGA CTGATGCTGC CAAGGAGCAG ATTCTAAAGA ACAGGAACAA AGTAATCTTG 
GGAAGAATAG AAAACCTCTT TAGAACATGT CATGCTATTG CCAAGACTGG ACGTCCCCTC 
AAAGACTTTG TCTGGATGTG TAAACTGGAT GATATGAAGG GTGTAGACGT TGGCCCAATA 
TTCAGGACTG ACAAGTCAGC CAGAACTTTC ATATACTTTA TTGCTGAAGT AGAACGCATA 
TGTCTTCGAG AGAAATTAGA AAAATGTAAG TTCTTCTCAC TCATCAGTGA TGGTGGCTCA 
GGCAGCTTCA TAGAAGACAC CAAAGTTGTT TACATCCAGT TTGCATATGA GGGCAGGGTC 
TGCTGTCAGG TTGTTGGAGT ACAGACTGTG GACAAAATTG AACCGGTGGC AATAAAAAGT 
GCCATTGAAA AAACACTTGA GATAAATCTT CAACTAAATC TGTCTAGCAA AGACTGGTCC 
AAAAAACTCG TTGGGTTTGG AAGTGATGGT GCATCTGTCA TGGTGGGAGA GAACAATGGT 
GTAGCCAGGC TTCTGAAAGA AATTCAGCCA TGTGTGCAAA CAGTCCACTG CTTTGCACAT 
AGACTTGAAC TAGCCTACCA AGAAGCATTG CAGAGCATCC AATTATACAG TACTATGAAT 
GATCTTCTCC AGAATATATA TTATTTTTAT CATGATTCAC CTTCAAATAC AAAAGCTCTG 
ATAGCTGCCT TTGAAGACCT TAAAATCCGC CCTGTGATTC CCTCTCGAGT TGGCAACAAC 
AGATGGCTCC CTCGGTTACA GATGGCTCTA CAAATCTTTC TCAAAGGATA TTCTCCAATT 
GTCAAGCAAT TAAGAACAAT GCATGAAGGC AGAAGTTCCG CTGATCAACA GAAAGCTCAA 
GAGCTCCTAC AGTTCCTTCT CCAAGCTGAG GTTGTCAGAT TTGCTCACTT CTTGCTGGAT 
GTAATCAACG TCCTCAGCAT TTTGTCTCGA GTCAATCAGG ACAGAAATTC ATCAATAGGA 
GATGTATTTG CAACCCTACA ATCAACACTA GGAACACTCA AGATGTATAA AAAAAGACCA 
GGTCCAAAGG AACGTCTGAC AGAGTCAGCC ACACACTTTC ATGGTAACCA ACTGACAGGA 
AATGGAAATA TCTCTGAAGT ACGAAACACA GTCTTAACAA ATCTCTCAAA GCATCTTCAT 
GATTGTTTCA GTGATGCCAG CCATGATGTG GTGAGGGCGA CCACTATTGG AAGTTTCAAA 
TTATGGCCAA GAAAGCTAGA TCAAGAGTTT GGTGAGAAAG AGGTAACAAT TCTTACAAAG 
CATTATGAAC CTGTGTTGGA AGGGGCCAAT GTGAACATTG ATGAAGTGGA CACAGAATGG 
AGCATGATGA AATTAGAGAT TTATAATCGA TTTCAGAACA TCCGAAAGTT AACCTGGGAT 
ATTGTGAATT CCATCTACTC ATACAAGTAT CCCAATATAT TGACCCTAGT GGATCTGGTG 
CTGACACTCC CTGCAAGTTC AGCTGAGGCG GAACGTGGGT TCAGTCAGAT GAAACTAGCC 
AAGTCACAAA TGCATTTTAA AACAGATCCT GAGAACATGA CAGACATTTT AATAATCCAG 
TTGAATTCTC CAGACATCAA TAAGTTTGAT CCCAAGAAAG CCATCCATCT ATGGAATACT 
TCTTGGCAGA GAAATACACT TCCACCAATT GACCACACAA TAACCAAGTC AGATTGCTCC 
TCTAGCTCAG ATAGTCTTTA TGAAAGTGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_016431609.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016287095.1 Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.

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CloneID	OMc41044
Clone ID Related Accession (Same CDS sequence)	XM_016431609.1
Accession Version	XM_016431609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	sperm flagellar protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008803.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACA TCCTGTGCAG GTGGCTAAAC CAGGAGGTGA AGCTGTCCCG GACAGTGAGT 
TCAAAGGGGA TTGAAAAAGA TTTTTCTAAT GGCTTCCTAC TGGGTGAACT TCTGCACAAA 
TATGAACTTC AGGATGACTT TCCTCTGTTT TCAGAAGGCA GGGTTTCCAA TGCAAAACTT 
AATAATTTTT CTCGCTTAGA ACCAACATTT CAACTCCTGG GTGTTCAGTT CGATCAAAAT 
GTGGCCCAGA ATATTATGAC AGAAAAGCAT GGCTCTGTAA CCATACTTCT TTATCAGTTG 
TACATTGCGC TTCAAAAAAA GAGAAAAAGT GGACTCACGG GAGTAGCAAT GCAAACAATG 
AGACCTTTAG CACCTGCAAA GCTACAAACT ATGAGAAGTG AGATTTATCG AGAGAGACTT 
AAGAACCTGG TGCCTCGGCA AACTGATTTG AATCTAAGTC GGGTTTCAGA AAAGTTTGAA 
GCAAAGTATA AAAAAATGGA AGAAAAAATT GCTCGTGTTC ATTTTCAAAA TATCCGAAAA 
TTTGAAAAGT TCCAGGAAGA ACAAAGAATT CAAGATCTTG AAAAGCAGCG CCTGGGAAGA 
AGACGACAAC ATGAAATGAT GGCTAGAATC CAAGCAGCCA TTTTACAGAT TCCCAAAACA 
GTACCCAATC GTACTCTGAA ATCTATTGAG AGCCACAAAA TGATGATGAA GAAAAAAGAG 
GAAGAAGATG TGCTGAATGA AATTCAGAAG TTTGAAATGC TAATAAAAAA GGAGCTTGAG 
GCCAAAGGCA GTGGATCAAA AGTTCCTTCG GCAGCCCAGA CAACTGCTGA CTTGTTAAGC 
ACGTATTCAG ATGAGGAATA CATTAAAAAA ATTCAAAAAC GTCTAGAGGA AGATACCTTA 
GCCCGGGAAC AAAGGGAGAA AAGACGCAGA AAATTGTTAA TGGATCAATT GATAGCCCAT 
GAAGCTCAAG AGGAGGCTTA CCGAGAAGAA CAACTCATTA ACCGACTAAT GAGACAATCT 
CAGCAGGAAC GTCGGATTGC TGTGCAACTC ATGCATGTTC GACACGAAAA GGAAGTTTTA 
TGGCAAAACA GGGTTTTCAG AGAAAAACAA TATGAAGAAA GACGGCTGAA AGATTTCCAG 
GATGCTCTTG ACAGAGAAGC AGCTCTTGCA AGACAAGCCA AGCTGGAGTA TGAAGAACAA 
GCCCAAAAGG AAATAGAAAT TCATGAAAGA ATAGCAGCAG AAAGATCTCA AGCTCGTTAT 
AAAAAGCATT ATTCAATGTG CACAGAAATC TTGGACAATA TCATTGATTT ATCAACTAAA 
ATAGCAGAAT ATCGAGAATT AACACATAAT CTTATTCCAG TTAAAATTAT GCGTGATTGG 
AAGGAGCTGT TTTTCAGTGG AAAGCCAATT TATGAAATAG CCTCCATTGA CTCCAAGCCA 
TCTCGTTTTA CCTCAGAACA ACTAATTGAG CTATCCAAAA GGAATTTACT AGATGACAAT 
GATTATGAGG AGTACAAGAA CTTGACAGGA GAGTGGGTCC TCCCAGAAGA TATGGCTGAT 
GTTCCCCTAC CTTCCAACAA TAACATATTG GGTCATGTGA TTCGAGTAGT TCATGAAAAA 
GTTTACCCTC CAAAACCAGC ATATATTGCA ACTCAGTTAC CTTCATCTTC GATTAAAGGG 
TGCATGCTGG GCAAGACATG CAGTGGGAAA ACCAGCTGTC TAAAGATTTT AGAAGAAGTT 
TTCCATATTG AGATACTTTG TATGGATACT CTGGTTCAAG AGGCTATTCA GGCTTTCCTT 
AGAAATGAAA CTGTAACTGA GGTGGAAACA CATAAAGAAG ATTTATCAAA AGAAGTTCAA 
GATATTAAGG ATTCAAACAT GACGACTATT GCAGAAATAA TTCCTACTGA GGAGAAAAAA 
GATGAGAATC TACCACAACA AGATGAAGTG ATTATTAAAC CACCATCAGC CCAGGATGTG 
AGAATAAGAC TTACTAATCG TGCTCAACTT GGTTCAAAAT GTGAAAAGTT GCTCAAGAGA 
GGAAGAAGTA TCCCCGATGT ACTGCTAATT CAAATCATGG TGAATGCTAT TAATGAAATA 
TCATCAGACA AAGGATGGAT CTTGGATGGT TTTCCAATGA CCATAAATCA AGCAAAACTT 
CTTGAGGAAG CCCTTACAGG GTTCAACACA GATCAACCAG AACATGAAAT AAAGGCTCAC 
AAAAAATCAA CATTGGTTTT TGATCCATCT GCTCCCAAAG ACCCCCCTCC TCCCCCACCT 
GCATTTGATT TTGTTATGTT GCTGGATATA ACTGACACAT TGGTGCTGAA TCGTGTGATG 
GGAGAGGAGA AAAGAAACAC TGACACAACT GGTATACCAG AAAGTGCAAC TTCAGAAATG 
TTTCCTGAAG AACAAGAAAA GTTTGAACTT TTGAGGGATC AGATACAACA CAGAATTTTA 
GGTTTCTTGG ACAACTGGCC AGAGCTGGAA AAATGGTTTT CAGGAAAACA AGACATTCTA 
ATCACAGTCA ATGCAGAAGT AGATAAAAGG GCTTTAGCCA CAAAACTACA AGAGCTTTTT 
ATTGCTGAAC TGAGGAAAAA AGAAGCTAAA GCAAAACAGG AATTAGAAGA ACAGGAAGAG 
GAAAGGAAAA AGAAAGAAGC CCTCCCACCT GAACCGCCAC CTCCACCTCC CCCTGAACCA 
GAAGAAGAGC CAAAGGAAGC AAAAGGGAAA GGAAAAGGGA AAGGTAAAGG TGCTAAAGAA 
GGAAAAGGAA AGAAAGACAC TTCTCCCAAG GGCAAAGATG CTGCTAAAGA AGCAAAAGCA 
AAGAAAAGTG AAACTTCTCC AAAAGGCAAA GGAAGGTCAC CAGACAAGAG GTCACCTGAA 
GATAAAACAT CACCTCCTGC ACCTCCAGGA CCACCACCAG TCAAAGCAGG ATCAGAAGAC 
TGGGTCTATG TGGATGAGCC ACTTCCTCAG GAAATATTTG AATATCTAGA CACTTTTTGG 
GAAGTAATAG AAAGTTCATA CATAAATACC AGCAAAGCTA TATTCAGACA GATAAGAGAT 
GAGAGACATA GTGTACTTTA TTATCTACAT AATGTCAGAC TAAATTACCA GGGATATCTG 
AAACGGCCAG ATCACAAGCA GGAATTTGTA TCACAATGGC AGGAGGATTT TAACAACCTC 
CCTGAAGACT TATGGGAGGA TGAGGAAACA AAAGCTGAAC TACACCAAAG AGTCAATGAT 
TTAAGGGACC GCCTGTGGGA TATATGTGAT ACTCGAAAGG AAGAGGCAGA ACAAGAACGT 
GCTGATATCA TTGATGAATC CTGGTTACAA GATCATCTTG GAATTCTTAT GAATCATTTC 
TTTTCTTTGA TGCAGGTGGA ACTTAGCCGT TTCCAGGACA TAAAGACTTT TCTACAAGAT 
TACTATAGGG GAATGGAAGG AAAAATCCCC ACCGACCTTG CTAAATCTGG ATATACTCGA 
GTTCCCCTTA TAGATCTTGA TGAAGAGGAT ACCGATATCA CCTCCAAAAT CCCTGTAATT 
CCTCGGAGAC AGCCTTCTCC TGAAGGACTA TTAAACAAGC CCAGAAGTAA ACTATCCCTG 
AGAGGAGGCA GACCTGATTA CTGCTTAGAA AATGTCCAAC TCAAATTTGA AAGAGATGAG 
AAGACCCTTA TGACTATATG GCAGAATACA TCTTCAGCCA TCAACCTCTT GGTAAATTCT 
GAAATACAGT TGAGGAAACT AGAAGATGAT AAAGAAAACA AACCAACATC AACTAAAGAA 
AAATCTCCTC AAGATGGTGG CAAAGGTCCT AAAGGTGCTA AGAAAAAGCC AGATAAGAAG 
AAAGTTCCAT CACCAGTTGA AGCCGTTTCT ATGGTTCTAA CACCAGAAGA ACAGGAGGCT 
CTTGATAAGA GGAATGAATT GAAAATGAAA ATAAAGGAAG AACATATTAC AGCTCTTCAG 
TGTGAAGCAC AAGCTTCAAT ATTCCGACTG GAACTGATCA AGATGAAGGC CTTATCTTAC 
ATGGAAGAGC TGCTAGGAAG AGCAGTTGAG GTTTACAAAC TGATGGACAA ATGGCTCGGA 
GCCCGATACT TAGCTGAAAT GGCAAGTGTG GAAAAATTAA CTGAAGTGGC TCACTACCAC 
ATTGAAACCT CAACAAGGAT TCAGAATGAA CTTATTTTAA ATGAAGAAGA TTTCTACATC 
AATGGTGATG TGAAAGTCTT TCCAGATCCG CTTCCTCCCG TCCGGCCTCC ACCTGTGGAG 
AAAGAAATGA ATACTTTTCT CACTCTTGAA CAACTCAACA CCCTTCGAAA TGAGTTTCTC 
CAGATGGCAC CAAAAGCCAA AACACCATGG AAGTATTTTA AAAAAGGAAT CTCTTCAGTT 
GATACTAGAA GACATGCTGA GGAAAAGAAA AGGCTAAGAA ATCTTGACTA TTACCTACCT 
AAGAGGAACA ACCAATTTTT GAAAATTTGG CTAAAAAAAT ACCCTTGGCT CGTGTATGAT 
GACAGGTCAG ACTTAATGTT CTGTGGCTTG TGTCGCAAAC ATGGAGTGAG CTCAGGAGGA 
AACCATGTTA GCTTTTTTTA TGGCACCAAC AACTTTAGGA CTGAATTTCT CCAAGCACAT 
GACAATAGTG AGGCACACAC GAAGGCTGTT CTAGCAGAAG CAACAGGGGT TTCTTCCCTG 
ACTGATGCTG CCAAGGAGCA GATTCTAAAG AACAGGAACA AAGTAATCTT GGGAAGAATA 
GAAAACCTCT TTAGAACATG TCATGCTATT GCCAAGACTG GACGTCCCCT CAAAGACTTT 
GTCTGGATGT GTAAACTGGA TGATATGAAG GGTGTAGACG TTGGCCCAAT ATTCAGGACT 
GACAAGTCAG CCAGAACTTT CATATACTTT ATTGCTGAAG TAGAACGCAT ATGTCTTCGA 
GAGAAATTAG AAAAATGTAA GTTCTTCTCA CTCATCAGTG ATGGTGGCTC AGGCAGCTTC 
ATAGAAGACA CCAAAGTTGT TTACATCCAG TTTGCATATG AGGGCAGGGT CTGCTGTCAG 
GTTGTTGGAG TACAGACTGT GGACAAAATT GAACCGGTGG CAATAAAAAG TGCCATTGAA 
AAAACACTTG AGATAAATCT TCAACTAAAT CTGTCTAGCA AAGACTGGTC CAAAAAACTC 
GTTGGGTTTG GAAGTGATGG TGCATCTGTC ATGGTGGGAG AGAACAATGG TGTAGCCAGG 
CTTCTGAAAG AAATTCAGCC ATGTGTGCAA ACAGTCCACT GCTTTGCACA TAGACTTGAA 
CTAGCCTACC AAGAAGCATT GCAGAGCATC CAATTATACA GTACTATGAA TGATCTTCTC 
CAGAATATAT ATTATTTTTA TCATGATTCA CCTTCAAATA CAAAAGCTCT GATAGCTGCC 
TTTGAAGACC TTAAAATCCG CCCTGTGATT CCCTCTCGAG TTGGCAACAA CAGATGGCTC 
CCTCGGTTAC AGATGGCTCT ACAAATCTTT CTCAAAGGAT ATTCTCCAAT TGTCAAGCAA 
TTAAGAACAA TGCATGAAGG CAGAAGTTCC GCTGATCAAC AGAAAGCTCA AGAGCTCCTA 
CAGTTCCTTC TCCAAGCTGA GGTTGTCAGA TTTGCTCACT TCTTGCTGGA TGTAATCAAC 
GTCCTCAGCA TTTTGTCTCG AGTCAATCAG GACAGAAATT CATCAATAGG AGATGTATTT 
GCAACCCTAC AATCAACACT AGGAACACTC AAGATGTATA AAAAAAGACC AGGTCCAAAG 
GAACGTCTGA CAGAGTCAGC CACACACTTT CATGGTAACC AACTGACAGG AAATGGAAAT 
ATCTCTGAAG TACGAAACAC AGTCTTAACA AATCTCTCAA AGCATCTTCA TGATTGTTTC 
AGTGATGCCA GCCATGATGT GGTGAGGGCG ACCACTATTG GAAGTTTCAA ATTATGGCCA 
AGAAAGCTAG ATCAAGAGTT TGGTGAGAAA GAGGTAACAA TTCTTACAAA GCATTATGAA 
CCTGTGTTGG AAGGGGCCAA TGTGAACATT GATGAAGTGG ACACAGAATG GAGCATGATG 
AAATTAGAGA TTTATAATCG GTTATTTGGG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016287095.1
CDS	82..6177
Translation	MSDILCRWLNQEVKLSRTVSSKGIEKDFSNGFLLGELLHKYELQDDFPLFSEGRVSNAKLNNFSRLEPTFQLLGVQFDQNVAQNIMTEKHGSVTILLYQLYIALQKKRKSGLTGVAMQTMRPLAPAKLQTMRSEIYRERLKNLVPRQTDLNLSRVSEKFEAKYKKMEEKIARVHFQNIRKFEKFQEEQRIQDLEKQRLGRRRQHEMMARIQAAILQIPKTVPNRTLKSIESHKMMMKKKEEEDVLNEIQKFEMLIKKELEAKGSGSKVPSAAQTTADLLSTYSDEEYIKKIQKRLEEDTLAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRVFREKQYEERRLKDFQDALDREAALARQAKLEYEEQAQKEIEIHERIAAERSQARYKKHYSMCTEILDNIIDLSTKIAEYRELTHNLIPVKIMRDWKELFFSGKPIYEIASIDSKPSRFTSEQLIELSKRNLLDDNDYEEYKNLTGEWVLPEDMADVPLPSNNNILGHVIRVVHEKVYPPKPAYIATQLPSSSIKGCMLGKTCSGKTSCLKILEEVFHIEILCMDTLVQEAIQAFLRNETVTEVETHKEDLSKEVQDIKDSNMTTIAEIIPTEEKKDENLPQQDEVIIKPPSAQDVRIRLTNRAQLGSKCEKLLKRGRSIPDVLLIQIMVNAINEISSDKGWILDGFPMTINQAKLLEEALTGFNTDQPEHEIKAHKKSTLVFDPSAPKDPPPPPPAFDFVMLLDITDTLVLNRVMGEEKRNTDTTGIPESATSEMFPEEQEKFELLRDQIQHRILGFLDNWPELEKWFSGKQDILITVNAEVDKRALATKLQELFIAELRKKEAKAKQELEEQEEERKKKEALPPEPPPPPPPEPEEEPKEAKGKGKGKGKGAKEGKGKKDTSPKGKDAAKEAKAKKSETSPKGKGRSPDKRSPEDKTSPPAPPGPPPVKAGSEDWVYVDEPLPQEIFEYLDTFWEVIESSYINTSKAIFRQIRDERHSVLYYLHNVRLNYQGYLKRPDHKQEFVSQWQEDFNNLPEDLWEDEETKAELHQRVNDLRDRLWDICDTRKEEAEQERADIIDESWLQDHLGILMNHFFSLMQVELSRFQDIKTFLQDYYRGMEGKIPTDLAKSGYTRVPLIDLDEEDTDITSKIPVIPRRQPSPEGLLNKPRSKLSLRGGRPDYCLENVQLKFERDEKTLMTIWQNTSSAINLLVNSEIQLRKLEDDKENKPTSTKEKSPQDGGKGPKGAKKKPDKKKVPSPVEAVSMVLTPEEQEALDKRNELKMKIKEEHITALQCEAQASIFRLELIKMKALSYMEELLGRAVEVYKLMDKWLGARYLAEMASVEKLTEVAHYHIETSTRIQNELILNEEDFYINGDVKVFPDPLPPVRPPPVEKEMNTFLTLEQLNTLRNEFLQMAPKAKTPWKYFKKGISSVDTRRHAEEKKRLRNLDYYLPKRNNQFLKIWLKKYPWLVYDDRSDLMFCGLCRKHGVSSGGNHVSFFYGTNNFRTEFLQAHDNSEAHTKAVLAEATGVSSLTDAAKEQILKNRNKVILGRIENLFRTCHAIAKTGRPLKDFVWMCKLDDMKGVDVGPIFRTDKSARTFIYFIAEVERICLREKLEKCKFFSLISDGGSGSFIEDTKVVYIQFAYEGRVCCQVVGVQTVDKIEPVAIKSAIEKTLEINLQLNLSSKDWSKKLVGFGSDGASVMVGENNGVARLLKEIQPCVQTVHCFAHRLELAYQEALQSIQLYSTMNDLLQNIYYFYHDSPSNTKALIAAFEDLKIRPVIPSRVGNNRWLPRLQMALQIFLKGYSPIVKQLRTMHEGRSSADQQKAQELLQFLLQAEVVRFAHFLLDVINVLSILSRVNQDRNSSIGDVFATLQSTLGTLKMYKKRPGPKERLTESATHFHGNQLTGNGNISEVRNTVLTNLSKHLHDCFSDASHDVVRATTIGSFKLWPRKLDQEFGEKEVTILTKHYEPVLEGANVNIDEVDTEWSMMKLEIYNRLFGK

Target ORF information:

RefSeq Version	XM_016431609.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016431609.1

ORF Insert Sequence:

ATGTCGGACA TCCTGTGCAG GTGGCTAAAC CAGGAGGTGA AGCTGTCCCG GACAGTGAGT 
TCAAAGGGGA TTGAAAAAGA TTTTTCTAAT GGCTTCCTAC TGGGTGAACT TCTGCACAAA 
TATGAACTTC AGGATGACTT TCCTCTGTTT TCAGAAGGCA GGGTTTCCAA TGCAAAACTT 
AATAATTTTT CTCGCTTAGA ACCAACATTT CAACTCCTGG GTGTTCAGTT CGATCAAAAT 
GTGGCCCAGA ATATTATGAC AGAAAAGCAT GGCTCTGTAA CCATACTTCT TTATCAGTTG 
TACATTGCGC TTCAAAAAAA GAGAAAAAGT GGACTCACGG GAGTAGCAAT GCAAACAATG 
AGACCTTTAG CACCTGCAAA GCTACAAACT ATGAGAAGTG AGATTTATCG AGAGAGACTT 
AAGAACCTGG TGCCTCGGCA AACTGATTTG AATCTAAGTC GGGTTTCAGA AAAGTTTGAA 
GCAAAGTATA AAAAAATGGA AGAAAAAATT GCTCGTGTTC ATTTTCAAAA TATCCGAAAA 
TTTGAAAAGT TCCAGGAAGA ACAAAGAATT CAAGATCTTG AAAAGCAGCG CCTGGGAAGA 
AGACGACAAC ATGAAATGAT GGCTAGAATC CAAGCAGCCA TTTTACAGAT TCCCAAAACA 
GTACCCAATC GTACTCTGAA ATCTATTGAG AGCCACAAAA TGATGATGAA GAAAAAAGAG 
GAAGAAGATG TGCTGAATGA AATTCAGAAG TTTGAAATGC TAATAAAAAA GGAGCTTGAG 
GCCAAAGGCA GTGGATCAAA AGTTCCTTCG GCAGCCCAGA CAACTGCTGA CTTGTTAAGC 
ACGTATTCAG ATGAGGAATA CATTAAAAAA ATTCAAAAAC GTCTAGAGGA AGATACCTTA 
GCCCGGGAAC AAAGGGAGAA AAGACGCAGA AAATTGTTAA TGGATCAATT GATAGCCCAT 
GAAGCTCAAG AGGAGGCTTA CCGAGAAGAA CAACTCATTA ACCGACTAAT GAGACAATCT 
CAGCAGGAAC GTCGGATTGC TGTGCAACTC ATGCATGTTC GACACGAAAA GGAAGTTTTA 
TGGCAAAACA GGGTTTTCAG AGAAAAACAA TATGAAGAAA GACGGCTGAA AGATTTCCAG 
GATGCTCTTG ACAGAGAAGC AGCTCTTGCA AGACAAGCCA AGCTGGAGTA TGAAGAACAA 
GCCCAAAAGG AAATAGAAAT TCATGAAAGA ATAGCAGCAG AAAGATCTCA AGCTCGTTAT 
AAAAAGCATT ATTCAATGTG CACAGAAATC TTGGACAATA TCATTGATTT ATCAACTAAA 
ATAGCAGAAT ATCGAGAATT AACACATAAT CTTATTCCAG TTAAAATTAT GCGTGATTGG 
AAGGAGCTGT TTTTCAGTGG AAAGCCAATT TATGAAATAG CCTCCATTGA CTCCAAGCCA 
TCTCGTTTTA CCTCAGAACA ACTAATTGAG CTATCCAAAA GGAATTTACT AGATGACAAT 
GATTATGAGG AGTACAAGAA CTTGACAGGA GAGTGGGTCC TCCCAGAAGA TATGGCTGAT 
GTTCCCCTAC CTTCCAACAA TAACATATTG GGTCATGTGA TTCGAGTAGT TCATGAAAAA 
GTTTACCCTC CAAAACCAGC ATATATTGCA ACTCAGTTAC CTTCATCTTC GATTAAAGGG 
TGCATGCTGG GCAAGACATG CAGTGGGAAA ACCAGCTGTC TAAAGATTTT AGAAGAAGTT 
TTCCATATTG AGATACTTTG TATGGATACT CTGGTTCAAG AGGCTATTCA GGCTTTCCTT 
AGAAATGAAA CTGTAACTGA GGTGGAAACA CATAAAGAAG ATTTATCAAA AGAAGTTCAA 
GATATTAAGG ATTCAAACAT GACGACTATT GCAGAAATAA TTCCTACTGA GGAGAAAAAA 
GATGAGAATC TACCACAACA AGATGAAGTG ATTATTAAAC CACCATCAGC CCAGGATGTG 
AGAATAAGAC TTACTAATCG TGCTCAACTT GGTTCAAAAT GTGAAAAGTT GCTCAAGAGA 
GGAAGAAGTA TCCCCGATGT ACTGCTAATT CAAATCATGG TGAATGCTAT TAATGAAATA 
TCATCAGACA AAGGATGGAT CTTGGATGGT TTTCCAATGA CCATAAATCA AGCAAAACTT 
CTTGAGGAAG CCCTTACAGG GTTCAACACA GATCAACCAG AACATGAAAT AAAGGCTCAC 
AAAAAATCAA CATTGGTTTT TGATCCATCT GCTCCCAAAG ACCCCCCTCC TCCCCCACCT 
GCATTTGATT TTGTTATGTT GCTGGATATA ACTGACACAT TGGTGCTGAA TCGTGTGATG 
GGAGAGGAGA AAAGAAACAC TGACACAACT GGTATACCAG AAAGTGCAAC TTCAGAAATG 
TTTCCTGAAG AACAAGAAAA GTTTGAACTT TTGAGGGATC AGATACAACA CAGAATTTTA 
GGTTTCTTGG ACAACTGGCC AGAGCTGGAA AAATGGTTTT CAGGAAAACA AGACATTCTA 
ATCACAGTCA ATGCAGAAGT AGATAAAAGG GCTTTAGCCA CAAAACTACA AGAGCTTTTT 
ATTGCTGAAC TGAGGAAAAA AGAAGCTAAA GCAAAACAGG AATTAGAAGA ACAGGAAGAG 
GAAAGGAAAA AGAAAGAAGC CCTCCCACCT GAACCGCCAC CTCCACCTCC CCCTGAACCA 
GAAGAAGAGC CAAAGGAAGC AAAAGGGAAA GGAAAAGGGA AAGGTAAAGG TGCTAAAGAA 
GGAAAAGGAA AGAAAGACAC TTCTCCCAAG GGCAAAGATG CTGCTAAAGA AGCAAAAGCA 
AAGAAAAGTG AAACTTCTCC AAAAGGCAAA GGAAGGTCAC CAGACAAGAG GTCACCTGAA 
GATAAAACAT CACCTCCTGC ACCTCCAGGA CCACCACCAG TCAAAGCAGG ATCAGAAGAC 
TGGGTCTATG TGGATGAGCC ACTTCCTCAG GAAATATTTG AATATCTAGA CACTTTTTGG 
GAAGTAATAG AAAGTTCATA CATAAATACC AGCAAAGCTA TATTCAGACA GATAAGAGAT 
GAGAGACATA GTGTACTTTA TTATCTACAT AATGTCAGAC TAAATTACCA GGGATATCTG 
AAACGGCCAG ATCACAAGCA GGAATTTGTA TCACAATGGC AGGAGGATTT TAACAACCTC 
CCTGAAGACT TATGGGAGGA TGAGGAAACA AAAGCTGAAC TACACCAAAG AGTCAATGAT 
TTAAGGGACC GCCTGTGGGA TATATGTGAT ACTCGAAAGG AAGAGGCAGA ACAAGAACGT 
GCTGATATCA TTGATGAATC CTGGTTACAA GATCATCTTG GAATTCTTAT GAATCATTTC 
TTTTCTTTGA TGCAGGTGGA ACTTAGCCGT TTCCAGGACA TAAAGACTTT TCTACAAGAT 
TACTATAGGG GAATGGAAGG AAAAATCCCC ACCGACCTTG CTAAATCTGG ATATACTCGA 
GTTCCCCTTA TAGATCTTGA TGAAGAGGAT ACCGATATCA CCTCCAAAAT CCCTGTAATT 
CCTCGGAGAC AGCCTTCTCC TGAAGGACTA TTAAACAAGC CCAGAAGTAA ACTATCCCTG 
AGAGGAGGCA GACCTGATTA CTGCTTAGAA AATGTCCAAC TCAAATTTGA AAGAGATGAG 
AAGACCCTTA TGACTATATG GCAGAATACA TCTTCAGCCA TCAACCTCTT GGTAAATTCT 
GAAATACAGT TGAGGAAACT AGAAGATGAT AAAGAAAACA AACCAACATC AACTAAAGAA 
AAATCTCCTC AAGATGGTGG CAAAGGTCCT AAAGGTGCTA AGAAAAAGCC AGATAAGAAG 
AAAGTTCCAT CACCAGTTGA AGCCGTTTCT ATGGTTCTAA CACCAGAAGA ACAGGAGGCT 
CTTGATAAGA GGAATGAATT GAAAATGAAA ATAAAGGAAG AACATATTAC AGCTCTTCAG 
TGTGAAGCAC AAGCTTCAAT ATTCCGACTG GAACTGATCA AGATGAAGGC CTTATCTTAC 
ATGGAAGAGC TGCTAGGAAG AGCAGTTGAG GTTTACAAAC TGATGGACAA ATGGCTCGGA 
GCCCGATACT TAGCTGAAAT GGCAAGTGTG GAAAAATTAA CTGAAGTGGC TCACTACCAC 
ATTGAAACCT CAACAAGGAT TCAGAATGAA CTTATTTTAA ATGAAGAAGA TTTCTACATC 
AATGGTGATG TGAAAGTCTT TCCAGATCCG CTTCCTCCCG TCCGGCCTCC ACCTGTGGAG 
AAAGAAATGA ATACTTTTCT CACTCTTGAA CAACTCAACA CCCTTCGAAA TGAGTTTCTC 
CAGATGGCAC CAAAAGCCAA AACACCATGG AAGTATTTTA AAAAAGGAAT CTCTTCAGTT 
GATACTAGAA GACATGCTGA GGAAAAGAAA AGGCTAAGAA ATCTTGACTA TTACCTACCT 
AAGAGGAACA ACCAATTTTT GAAAATTTGG CTAAAAAAAT ACCCTTGGCT CGTGTATGAT 
GACAGGTCAG ACTTAATGTT CTGTGGCTTG TGTCGCAAAC ATGGAGTGAG CTCAGGAGGA 
AACCATGTTA GCTTTTTTTA TGGCACCAAC AACTTTAGGA CTGAATTTCT CCAAGCACAT 
GACAATAGTG AGGCACACAC GAAGGCTGTT CTAGCAGAAG CAACAGGGGT TTCTTCCCTG 
ACTGATGCTG CCAAGGAGCA GATTCTAAAG AACAGGAACA AAGTAATCTT GGGAAGAATA 
GAAAACCTCT TTAGAACATG TCATGCTATT GCCAAGACTG GACGTCCCCT CAAAGACTTT 
GTCTGGATGT GTAAACTGGA TGATATGAAG GGTGTAGACG TTGGCCCAAT ATTCAGGACT 
GACAAGTCAG CCAGAACTTT CATATACTTT ATTGCTGAAG TAGAACGCAT ATGTCTTCGA 
GAGAAATTAG AAAAATGTAA GTTCTTCTCA CTCATCAGTG ATGGTGGCTC AGGCAGCTTC 
ATAGAAGACA CCAAAGTTGT TTACATCCAG TTTGCATATG AGGGCAGGGT CTGCTGTCAG 
GTTGTTGGAG TACAGACTGT GGACAAAATT GAACCGGTGG CAATAAAAAG TGCCATTGAA 
AAAACACTTG AGATAAATCT TCAACTAAAT CTGTCTAGCA AAGACTGGTC CAAAAAACTC 
GTTGGGTTTG GAAGTGATGG TGCATCTGTC ATGGTGGGAG AGAACAATGG TGTAGCCAGG 
CTTCTGAAAG AAATTCAGCC ATGTGTGCAA ACAGTCCACT GCTTTGCACA TAGACTTGAA 
CTAGCCTACC AAGAAGCATT GCAGAGCATC CAATTATACA GTACTATGAA TGATCTTCTC 
CAGAATATAT ATTATTTTTA TCATGATTCA CCTTCAAATA CAAAAGCTCT GATAGCTGCC 
TTTGAAGACC TTAAAATCCG CCCTGTGATT CCCTCTCGAG TTGGCAACAA CAGATGGCTC 
CCTCGGTTAC AGATGGCTCT ACAAATCTTT CTCAAAGGAT ATTCTCCAAT TGTCAAGCAA 
TTAAGAACAA TGCATGAAGG CAGAAGTTCC GCTGATCAAC AGAAAGCTCA AGAGCTCCTA 
CAGTTCCTTC TCCAAGCTGA GGTTGTCAGA TTTGCTCACT TCTTGCTGGA TGTAATCAAC 
GTCCTCAGCA TTTTGTCTCG AGTCAATCAG GACAGAAATT CATCAATAGG AGATGTATTT 
GCAACCCTAC AATCAACACT AGGAACACTC AAGATGTATA AAAAAAGACC AGGTCCAAAG 
GAACGTCTGA CAGAGTCAGC CACACACTTT CATGGTAACC AACTGACAGG AAATGGAAAT 
ATCTCTGAAG TACGAAACAC AGTCTTAACA AATCTCTCAA AGCATCTTCA TGATTGTTTC 
AGTGATGCCA GCCATGATGT GGTGAGGGCG ACCACTATTG GAAGTTTCAA ATTATGGCCA 
AGAAAGCTAG ATCAAGAGTT TGGTGAGAAA GAGGTAACAA TTCTTACAAA GCATTATGAA 
CCTGTGTTGG AAGGGGCCAA TGTGAACATT GATGAAGTGG ACACAGAATG GAGCATGATG 
AAATTAGAGA TTTATAATCG GTTATTTGGG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SPEF2
Entrez Gene ID	103094680
Full Name	sperm flagellar 2
Gene Type	protein-coding
Organism	Monodelphis domestica(gray short-tailed opossum)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SPEF2 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene

Clones

gRNAs

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