LINJ_02_0470 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_02_0470
Entrez Gene ID	5066198
Full Name	putative glycerate kinase
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001462700.1	XP_001462737.1	putative glycerate kinase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_02_0470 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_02_0470 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc00222	XM_001462700.1 Latest version!	Leishmania infantum JPCM5 putative glycerate kinase partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_02_0470 ( XM_001462700.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001462737.1 Leishmania infantum JPCM5 putative glycerate kinase partial mRNA.

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CloneID	OLc00222
Clone ID Related Accession (Same CDS sequence)	XM_001462700.1
Accession Version	XM_001462700.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	putative glycerate kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009387). COMPLETENESS: incomplete on both ends.

ATGCCAACAG GCTGCGCGAG AGCATCATCA GCGATACAGC AGCCCGTATC GGTAACTCTG 
GAGGCATCGT CCTCGATGCC TCCGTCACCT GGCGCCGCTG CTGCTGCGGC GTCCGCTGCC 
ACTCGCTCCA TCGCCTCGCC AGTCCCACGA AGTCCTGCGC CCAAGCGCCA TGAAGCGCGT 
CAGGACGCTC CGCACCCGCA TCGTCCAGTG CAACCGCCGC TTCGAATATC GGCGCTGTCG 
GCCGCGATAC CGCATCTTGG ACAACTCCAC CACTCTCACC GCCACGGCGA CGGCGCCTCT 
CCGCTGCACG TGCTGTGTGC CTGCGACACC TTCAAGGGCA CCCTCCCCTC AGACAAAGTC 
GGTGAGGCGG TGGAGAAGGG TTACTGGCAG GCCTGGGCCA AGACGCGCAG CTCCGCAACG 
GCGCAGTCAC ACGTACTTCC AGCAGTCACG GCGCCGTCGG AGGAGGTGTC GGTGCCATCG 
CCCACGTCTT CACCGCTGCA TCTGGTGGTA CCGCCGCTGG CCGCAAGCTT GCTTTCTGCG 
CAGCACCCTT CTGTCCTCCC AGCAGACGCG GTGCGGTTTC TGCACACCCC AATGAGCGAC 
GGTGGCGCCG GTCTGCTAGA CAGCGTCACC TACGCCTCAA CGCACGCGAG CATGACGTGG 
GGCACCATGC CCAACCTGTC ACCGTCGGGC GGCGCGTTGC GCCTGCAGCG TGTATACGTT 
CCAGCGTCCG TCCCGATCAC AGGCCCCCTC GGTGCCGCGA TCACGACGGA CACCGGTGGT 
GCACGAAGCG CCGCACCAGC TTCCCGCAGG GTGTCCTTCG CCTGTGATGT GGAGAGACGC 
GTGCTTGTCG TTGAGATGGC AGAGGCGGCT GGGCTGACGC GCATCGCGCG TCCACAAGAC 
CGACACCCAG GCCGCACGAC CAGCTACGGT GTAGGTGAGC TTATCCGCTA CGCGTTGGCC 
TACATGGCTG AGGCTGCTCA TGTACAAGCG GAGGCAGACG TGGCCTCGAA AACGCCTTCC 
CTGGACAGCG CGCCGGGTAG CTGCGGGGGT GTGCGCCTGT TCCTGGGCAT CGGCGGGAGC 
TCCACCAACG ATGGCGGACT CGGTGCACTG CAGGCGCTGG GGCTGGAGGT CTTTGTGGAT 
GCTGCCCACG CCCGCGCGAC GGACTGTGGT CACGACGGTG CCCGACGCGC GCGCGCATCG 
ACGGCGGTGC CAGATGCTCA TGCAGCTCCG ACTCATGTTG ACGACGGCGC GGGGGTGCGC 
CTCGCGAGAC CCTTCCGTGG CGAGGACTTG GCCCACGTCA CCCGCGTCTG CATCGGCGCA 
GAGATGCAGT GCATCTTTCC ATACCTGCCT GAAGCAGCGA CGCCACCTAC CGAGACGGGT 
ACGGGAGGCG ATGATGCGCA TGCCGCACCA CCGCCGCACT CTTCACTGTC GCCTGCGCGC 
GCGTGCTACA TCACGGAGGT GTGCTTGATC TGCGACGTGG ACAACACGCT TGTTGGTGCG 
CGCGGGGCAA CGTATGCCTT TGGACCACAG AAGTGCGCCG CGCCACACGC CGCTGCTCCG 
TTGTCACCTG CCACAGCTGC CGGTACACCT CCTGCTGGAG CGCATGCGGA CAGTGCAGGG 
GTAGAAGCTG CCGAGGATGC GGCGGTCGGC ACCGTCACCT CTGCGGCGCA GCAGCAGATG 
CTGGACTCGC TAGAGGCGGG CATGCGGCAC GCTTCCGCAC GCATCGTGGC GAGCGTGTGG 
CGGCCCCTTG CCGCTGTGGA TGCGGCTTCG GCGGCCCACG CGCAACATGC CGCGGGGGAG 
GCTAGGCACA TGGCCGTTGG TTCGGATAAG GGAGATAGTG TGGCAGAGGC GGCGGTGCTG 
GAGGATCTGC TATACCGCCG AGGCGGTGGG GGCGCAGGGG GCATGAGCGG GTTCTTCCGC 
TATCTGCTGC TGGCGCGCTA CGTGCCTGGG GCGGACGTGG TTGGCGGTCT GCTTGGCCTT 
TATGAGACCC CGCAAGTCGC GCAGCTGCTG AGAAGTGAGG ACAGTGTTTC ATTAAGGCGG 
GCTGCGGCAA CCGCGGCGGA GAAGCGCGGC GGACAGCGCA GTGATGACGC GCTTCCGGTG 
CCGGCAGAAT GGTCCGCGCA CGTTTCTGGA CAGCCAACGG CGTTCCCCCT GCGATGCCCG 
CGCGGATGTC TGTTCCACAC CTGCGATGTC GTCGTGTCAG GCGAGGGCTC CTTCGACGAG 
CAGACAATTC TGTCGCACAA GACGGTGGGC CGGCTGCTGG AGATGTGCAC GGTGGCCAAC 
GCATACCGCT TGTGGCACCA CTACTGTGAC GACGTGGGGG AGGCTGCGGC GGCCTGGAGC 
GAAAGGCGCG GCTGCCGCTG TATCACCGAC GTGGTCGTCG TGTGCGGCCG CTGCGGCTTT 
GGCGACTACA CGCACTTGCA GTCCGCTGCG CTGCAAGCCG TGTGCACGAC TCTACTCCCG 
CTACACATCC CCAATCGCTG CGTGGCGACT GCTGCTGCGA CGTCGACGCT TCGGGAAGCA 
GACGGCGCCA ACGCTGATGA CACACGCCTG CATATGTATT TGCAGGACTT GATGCAGTCG 
TCGGCGTTTT GGCGCGCGTG CGGCGAGTCC TCTTCTGCCC CGGAGGACGA TCAAGGCTTG 
AAGTACTTGC TCTCGGCGTA CTGTGTGCCG CGTGTGACGG TGCTGCCGCT TACGCCGACG 
CTGTTCCCTG TGCCGGCGGC GATGCAGCAG CCGTACGCGT GCGTTGTTTC CGCAGTGGCG 
CACTTACTCG AAGAGTCAGT GAGGCGCGTG ACAAGCGCGC AAGAGGCAAG CGTGGATGCG 
TGTGGCAGGC ATGCGCGGAG CTGTCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001462737.1
CDS	1..2850
Translation	MPTGCARASSAIQQPVSVTLEASSSMPPSPGAAAAAASAATRSIASPVPRSPAPKRHEARQDAPHPHRPVQPPLRISALSAAIPHLGQLHHSHRHGDGASPLHVLCACDTFKGTLPSDKVGEAVEKGYWQAWAKTRSSATAQSHVLPAVTAPSEEVSVPSPTSSPLHLVVPPLAASLLSAQHPSVLPADAVRFLHTPMSDGGAGLLDSVTYASTHASMTWGTMPNLSPSGGALRLQRVYVPASVPITGPLGAAITTDTGGARSAAPASRRVSFACDVERRVLVVEMAEAAGLTRIARPQDRHPGRTTSYGVGELIRYALAYMAEAAHVQAEADVASKTPSLDSAPGSCGGVRLFLGIGGSSTNDGGLGALQALGLEVFVDAAHARATDCGHDGARRARASTAVPDAHAAPTHVDDGAGVRLARPFRGEDLAHVTRVCIGAEMQCIFPYLPEAATPPTETGTGGDDAHAAPPPHSSLSPARACYITEVCLICDVDNTLVGARGATYAFGPQKCAAPHAAAPLSPATAAGTPPAGAHADSAGVEAAEDAAVGTVTSAAQQQMLDSLEAGMRHASARIVASVWRPLAAVDAASAAHAQHAAGEARHMAVGSDKGDSVAEAAVLEDLLYRRGGGGAGGMSGFFRYLLLARYVPGADVVGGLLGLYETPQVAQLLRSEDSVSLRRAAATAAEKRGGQRSDDALPVPAEWSAHVSGQPTAFPLRCPRGCLFHTCDVVVSGEGSFDEQTILSHKTVGRLLEMCTVANAYRLWHHYCDDVGEAAAAWSERRGCRCITDVVVVCGRCGFGDYTHLQSAALQAVCTTLLPLHIPNRCVATAAATSTLREADGANADDTRLHMYLQDLMQSSAFWRACGESSSAPEDDQGLKYLLSAYCVPRVTVLPLTPTLFPVPAAMQQPYACVVSAVAHLLEESVRRVTSAQEASVDACGRHARSCL

Target ORF information:

RefSeq Version	XM_001462700.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative glycerate kinase partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001462700.1

ORF Insert Sequence:

ATGCCAACAG GCTGCGCGAG AGCATCATCA GCGATACAGC AGCCCGTATC GGTAACTCTG 
GAGGCATCGT CCTCGATGCC TCCGTCACCT GGCGCCGCTG CTGCTGCGGC GTCCGCTGCC 
ACTCGCTCCA TCGCCTCGCC AGTCCCACGA AGTCCTGCGC CCAAGCGCCA TGAAGCGCGT 
CAGGACGCTC CGCACCCGCA TCGTCCAGTG CAACCGCCGC TTCGAATATC GGCGCTGTCG 
GCCGCGATAC CGCATCTTGG ACAACTCCAC CACTCTCACC GCCACGGCGA CGGCGCCTCT 
CCGCTGCACG TGCTGTGTGC CTGCGACACC TTCAAGGGCA CCCTCCCCTC AGACAAAGTC 
GGTGAGGCGG TGGAGAAGGG TTACTGGCAG GCCTGGGCCA AGACGCGCAG CTCCGCAACG 
GCGCAGTCAC ACGTACTTCC AGCAGTCACG GCGCCGTCGG AGGAGGTGTC GGTGCCATCG 
CCCACGTCTT CACCGCTGCA TCTGGTGGTA CCGCCGCTGG CCGCAAGCTT GCTTTCTGCG 
CAGCACCCTT CTGTCCTCCC AGCAGACGCG GTGCGGTTTC TGCACACCCC AATGAGCGAC 
GGTGGCGCCG GTCTGCTAGA CAGCGTCACC TACGCCTCAA CGCACGCGAG CATGACGTGG 
GGCACCATGC CCAACCTGTC ACCGTCGGGC GGCGCGTTGC GCCTGCAGCG TGTATACGTT 
CCAGCGTCCG TCCCGATCAC AGGCCCCCTC GGTGCCGCGA TCACGACGGA CACCGGTGGT 
GCACGAAGCG CCGCACCAGC TTCCCGCAGG GTGTCCTTCG CCTGTGATGT GGAGAGACGC 
GTGCTTGTCG TTGAGATGGC AGAGGCGGCT GGGCTGACGC GCATCGCGCG TCCACAAGAC 
CGACACCCAG GCCGCACGAC CAGCTACGGT GTAGGTGAGC TTATCCGCTA CGCGTTGGCC 
TACATGGCTG AGGCTGCTCA TGTACAAGCG GAGGCAGACG TGGCCTCGAA AACGCCTTCC 
CTGGACAGCG CGCCGGGTAG CTGCGGGGGT GTGCGCCTGT TCCTGGGCAT CGGCGGGAGC 
TCCACCAACG ATGGCGGACT CGGTGCACTG CAGGCGCTGG GGCTGGAGGT CTTTGTGGAT 
GCTGCCCACG CCCGCGCGAC GGACTGTGGT CACGACGGTG CCCGACGCGC GCGCGCATCG 
ACGGCGGTGC CAGATGCTCA TGCAGCTCCG ACTCATGTTG ACGACGGCGC GGGGGTGCGC 
CTCGCGAGAC CCTTCCGTGG CGAGGACTTG GCCCACGTCA CCCGCGTCTG CATCGGCGCA 
GAGATGCAGT GCATCTTTCC ATACCTGCCT GAAGCAGCGA CGCCACCTAC CGAGACGGGT 
ACGGGAGGCG ATGATGCGCA TGCCGCACCA CCGCCGCACT CTTCACTGTC GCCTGCGCGC 
GCGTGCTACA TCACGGAGGT GTGCTTGATC TGCGACGTGG ACAACACGCT TGTTGGTGCG 
CGCGGGGCAA CGTATGCCTT TGGACCACAG AAGTGCGCCG CGCCACACGC CGCTGCTCCG 
TTGTCACCTG CCACAGCTGC CGGTACACCT CCTGCTGGAG CGCATGCGGA CAGTGCAGGG 
GTAGAAGCTG CCGAGGATGC GGCGGTCGGC ACCGTCACCT CTGCGGCGCA GCAGCAGATG 
CTGGACTCGC TAGAGGCGGG CATGCGGCAC GCTTCCGCAC GCATCGTGGC GAGCGTGTGG 
CGGCCCCTTG CCGCTGTGGA TGCGGCTTCG GCGGCCCACG CGCAACATGC CGCGGGGGAG 
GCTAGGCACA TGGCCGTTGG TTCGGATAAG GGAGATAGTG TGGCAGAGGC GGCGGTGCTG 
GAGGATCTGC TATACCGCCG AGGCGGTGGG GGCGCAGGGG GCATGAGCGG GTTCTTCCGC 
TATCTGCTGC TGGCGCGCTA CGTGCCTGGG GCGGACGTGG TTGGCGGTCT GCTTGGCCTT 
TATGAGACCC CGCAAGTCGC GCAGCTGCTG AGAAGTGAGG ACAGTGTTTC ATTAAGGCGG 
GCTGCGGCAA CCGCGGCGGA GAAGCGCGGC GGACAGCGCA GTGATGACGC GCTTCCGGTG 
CCGGCAGAAT GGTCCGCGCA CGTTTCTGGA CAGCCAACGG CGTTCCCCCT GCGATGCCCG 
CGCGGATGTC TGTTCCACAC CTGCGATGTC GTCGTGTCAG GCGAGGGCTC CTTCGACGAG 
CAGACAATTC TGTCGCACAA GACGGTGGGC CGGCTGCTGG AGATGTGCAC GGTGGCCAAC 
GCATACCGCT TGTGGCACCA CTACTGTGAC GACGTGGGGG AGGCTGCGGC GGCCTGGAGC 
GAAAGGCGCG GCTGCCGCTG TATCACCGAC GTGGTCGTCG TGTGCGGCCG CTGCGGCTTT 
GGCGACTACA CGCACTTGCA GTCCGCTGCG CTGCAAGCCG TGTGCACGAC TCTACTCCCG 
CTACACATCC CCAATCGCTG CGTGGCGACT GCTGCTGCGA CGTCGACGCT TCGGGAAGCA 
GACGGCGCCA ACGCTGATGA CACACGCCTG CATATGTATT TGCAGGACTT GATGCAGTCG 
TCGGCGTTTT GGCGCGCGTG CGGCGAGTCC TCTTCTGCCC CGGAGGACGA TCAAGGCTTG 
AAGTACTTGC TCTCGGCGTA CTGTGTGCCG CGTGTGACGG TGCTGCCGCT TACGCCGACG 
CTGTTCCCTG TGCCGGCGGC GATGCAGCAG CCGTACGCGT GCGTTGTTTC CGCAGTGGCG 
CACTTACTCG AAGAGTCAGT GAGGCGCGTG ACAAGCGCGC AAGAGGCAAG CGTGGATGCG 
TGTGGCAGGC ATGCGCGGAG CTGTCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_02_0470 cDNA ORF clone, Leishmania infantum JPCM5

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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