PIEZO2 cDNA ORF clone, Equus caballus(horse)

Gene
Clones
gRNAs

Summary

Gene Symbol	PIEZO2
Entrez Gene ID	100056227
Full Name	piezo type mechanosensitive ion channel component 2
Gene Type	protein-coding
Organism	Equus caballus(horse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014727615.1	XP_014583101.1	piezo-type mechanosensitive ion channel component 2
XM_023647473.1	XP_023503241.1	piezo-type mechanosensitive ion channel component 2 isoform X3
XM_023647471.1	XP_023503239.1	piezo-type mechanosensitive ion channel component 2 isoform X1
XM_023647472.1	XP_023503240.1	piezo-type mechanosensitive ion channel component 2 isoform X2

Pathways from BioSystems
Gene Ontology

The following PIEZO2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIEZO2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHb70016	XM_023647473.1 Latest version!	Equus caballus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHb32427	XM_014727615.1 Latest version!	Equus caballus piezo-type mechanosensitive ion channel component 2 (PIEZO2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHb70014	XM_023647471.1 Latest version!	Equus caballus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHb70015	XM_023647472.1 Latest version!	Equus caballus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIEZO2 ( XM_023647473.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023503241.1 Equus caballus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHb70016
Clone ID Related Accession (Same CDS sequence)	XM_023647473.1
Accession Version	XM_023647473.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	piezo-type mechanosensitive ion channel component 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009151.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGG AAGTGGTGTG CGGACTCATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
GTAGCGTGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTCATCTA CCTCTTGCTC 
ATCCCTCTGT TCTCAGAACC AACAAAAGCG ACGATGCAAG GACATACGGG ACGGTTGTTG 
AAGTCTCTGT GCTTCACCAG TCTGTCCTTC CTGTTGCTGC ACGTCATCTT TCACATCACA 
TTGGCCAGCC TGGAAGCTCA GCACCGTATC GCGCCTGGTT ACAACTGCTC GACGTGGGAA 
AAGACGCTTC GGCAGATTGG CTTTGAAAGC TTAAAGGGAG CCGACGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCGTCTGT 
AGGAACATTG TTCAGAAACC GGTGACAGAA GAAGCCGCAC AGTGTAACCC GGAGTTTCAA 
AATGAAGAAG TGGCTGGAGG AGAAAAAATA GATCCAGAAG AGGCACTGAT CTACGAAGAG 
GATTTAGATG AAGGAGATGG TGTTGAAGGA GAGTTGGAAG ACAGCACAAA ATTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCTTCCAAG CTTAAGGAGT TTATTGGCAA CATGATAACC 
ACCGCTGGAA AAGTTGTTGT GACAATTTTA CTGGGGTCAT CAGGTATGAT GCTGCCGTCT 
CTGACCTCGT CTGTGTACTT CTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACAGCA 
GGGCACTTAA TTGGACTTTA TTTATATCAG TTCCAATTCT TTCAAGAAGC GGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAATCAGTCA TTCAAACAGA CTGTTCCAGC 
ACGTGGAAGA TCATAGCGAA CCCAGACCTG TCCTGGTATC ACCACGCCAA CCCCATCCTC 
CTGCTGGTGA TGTACTACAC GCTGGCCACT CTCATCCGCA TCTGGCTACA GGAGCCGCTT 
GTGCAGGATG AGAGGACCAA GGAAGAGGAC AGAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACGGCCGAGA GGAGGCGGAG CCTGTGGTAC GCGACCCATT ACCCAACTGA CGAGAGAAAA 
CTTCTATCCA TGACTCAAGA TGACTACAAA CCATCTGATG GTCTGCTGGT GACTGTGAAC 
GGCAATCCTG TGGATTACCA CACCATCCAC CCCAGTCTTC CCATGGAGAA CGGCCCCGCC 
AAAGCCGACC TGTACTCCAC CCCGCAGTAC CGGTGGGAGC CCTCCGACGA CTCCTCGGAA 
AAGAAAGAGG AAGAAGAAGA TGAGAAAGAA GAATTTGAAG AGGAAAGGAG CCATGAGGAG 
AAAAGAAGTG TTAAAGTTCA CGCCATGGTC TCTGTGTTCC AGTTTATTAT GAAACAAAGT 
TACATCTGTG CCCTTATTGC TATGATGGCT TGGAGCATCA CCTATCACAG CTGGTTGACC 
TTCGTGCTGT TGATTTGGTC GTGCACTCTT TGGATGATTC GCAACAGGAG AAAATATGCC 
ATGATCAGCT CTCCTTTCAT GGTTGTTTAT GGAAATCTAC TGCTGATATT ACAGTATATA 
TGGAGTTTTG AACTTCCTGA AATTAAAAAG GTCCCAGGAT TTTTAGAAAA GAAAGAGCCA 
GGAGAACTTG CCTCAAAGAT ACTTTTCACC ATTACTTTTT GGCTACTGCT GAGGCAGCAC 
CTCACAGAGC AGAAAGCTCT TCGGGAAAAG GAAGCTCTTT TATCTGAGGT CAAAATTGGG 
AGTCAGGAAA ATGAAGAAAA AGCTGATGAG CAAGATATAC AGGTGGAAGG AGAGGCGGAA 
GAGAAGGAGG AAGGAGAGGA AGCAAAGCGA GAGAAGCAGG AGATAAAGAA GGAAGAGGAA 
GAGGAAGAGG AAGTGGAGGA AGGTGACGAT CAGGACATCA TGACGGTGCT GGGCAATCTG 
GTGGTGGCCA TGTTCATTAA GTACTGGATC TACGTCTGTG GAGGGATGTT CTTCTTCGTC 
AGCTTCGAGG GTAAAATTGT GATGTACAAA ATCATCTACA TGGTGCTGTT TCTATTCTGT 
GTGGCCTTGT ATCAGGTGCA CTATGAATGG TGGAGGCGAA TTCTAAAATA TTTTTGGATG 
TCAGTGGTTA TTTATACTAT GCTGGTGCTC ATCTTTATAT ACACATATCA GTTTGAAAAC 
TTCCCAGGCC TATGGCAAAA TATGACTGGA TTAAAAAGAG AAAAGCTTGA GGATCTTGGC 
TTAAAGCAGT TTACCGTGGC TGAACTATTC ACTCGCATAT TCATCCCAAC CTCCTTTCTC 
CTGGTGTGCA TTTTGCACCT TCACTATTTC CATGACCGGT TCCTGGAGCT CACAGACCTG 
AAGTCCATTC CCTGCAAGGA AGACAGCGCC ATCTACAGAC TGGCGCACCC TGAGGGAAGC 
CTGCCGGACC TCACCATGAT GAACCTGACC GCCAGCCTGC AGGCGCCGGA GATGGGGAAG 
CCGGCAGAGC CTGGTGCGGA GAAGCTGGAG GGGTGCCCTG AGAAAGCCGA GAAGGCTGAG 
CTTGGGAAAG ACAGTGAGGA GGAGGAGGAG GAGGAAGAGG AAGAGGAGGA GGCAGGGACG 
TCAGATTTAA GGAACAAATG GCACCTGGTG ATTGACCGCC TTACTGTGCT CTTCTTAAAA 
TTCCTGGAGT ATTTTCACAA GCTGCAGGTG TTCATGTGGT GGATTTTGGA GTTGCATATC 
ATCAAAATCG TTTCATCATA CATTATCTGG GTTTCTGTGA AAGAGGTGTC TCTGTTCAAC 
TATGTATTTT TGATTTCTTG GGCTTTTGCT CTGCCATATG CCAAGCTACG CCGTCTGGCT 
TCAAGTGTCT GCACTGTCTG GACGTGTGTG ATCATCGTCT GCAAAATGTT GTACCAGCTG 
CAAACCATTA AGCCTGAGAA CTTTTCTGTT AACTGTTCCT TGCCAAATGA AAACCAAACG 
AATGTCCCCC TCCAGCAGTT GAACAAGTCA CTGCTCTACA ACGCTCCTAT TGACCCGACA 
GAGTGGGTCG GCCTGAGGAA GTCCTCCCCT TTGCTGGTCT ACCTGAGGAA TAACCTCCTG 
ATGCTGGCCA TCCTGGCCTT CGAGGTTACA ATTTACCGCC ATCAGGAATA CTATCGAGGT 
CGGAACAATC TCACGGCCCC CGTGTCTAAA ACCATCTTTC ACGACATTAC GAGGCTCCAT 
CTAGATGATG GACTTATTAA TTGTGCCAAG TATTTCATAA ATTACTTCTT CTACAAGTTT 
GGCCTAGAGA CCTGTTTCCT AATGTCAGTG AACGTCATTG GGCAGCGAAT GGACTTCTAT 
GCCATGATTC ACGCCTGCTG GTTGATCGCT GTCTTATATC GACGCAGAAG AAAAGCCATT 
GCGGAGATCT GGCCCAAGTA CTGCTGCTTC CTGGCCTGCA TCATCACGTT CCAGTACTTC 
ATCTGCATCG GCATTCCTCC TGCTCCTTGC AGAGATTACC CGTGGAGATT CAAGGGTGCC 
AGCTTCAACG ACAACATCAT TAAGTGGCTG TACTTCCCAG ACTTCATTGT GCGGCCCAAC 
CCCGTGTTTC TCGTCTATGA CTTCATGCTG CTTCTGTGTG CCTCCTTACA AAGGCAAATT 
TTTGAGGATG AAAATAAGGC TGCAGTGCGA ATCATGGCGG GAGACAATGT CGAGATTTGC 
ATGAACCTTG ATGCAGCCTC TTTCAGCCAG CATAACCCTG TACCAGACTT CATTCACTGC 
AGGTCGTACT TAGACATGTC CAAAGTAATC ATCTTCAGCT ACCTCTTCTG GTTCGTCCTC 
ACGATCATCT TCATCACTGG GACCACCAGG ATCAGCATCT TTTGCATGGG TTACTTGGTG 
GCCTGCTTCT ATTTCCTGCT TTTTGGGGGC AATTTGCTGT TGAAACCCAT CAAGAGCATC 
CTGCGCTATT GGGACTGGCT GATTGCATAC AACGTTTTTG TGATTACAAT GAAAAACATA 
CTGTCAATAG GAGCATGTGG ATACATTGAA AAATTGGTTC AAAATAGTTG CTGGTTGATC 
CAAGCTTTCA GCCTGGCCTG CACCGTCAAA GGCTATAAAA TGCCTGATAA CGATTCCTCA 
TGTAAACTGC CCAGTGGTGA AGCAGGAATT ATCTGGGACA GCATCTGTTT TGCCTTCCTC 
CTGCTGCAGA GGAGAGTTTT CATGAGTTAC TATTTTCTAC ATGTCGTGGC AGATATAAAA 
GCTTCACAGA TCCTGGCATC AAGAGGGGCA GAACTTTTCC AGGCCACAAT TGTGAAGGCT 
GTAAAGGCCA GAATTGAGGA GGAGAAGAAG TCAATGGATC AGTTGAAGAG GCAGATGGAT 
CGCATCAAGG CCAGACAACA GAAATACAAA AAGGGTAAGG AGAGGATGCT GAGCTTGACT 
CAGGAGTCAG GGGAAGGCCA AGACATCCAA AAGCTCTCTG AAGAGGATGA TGAAAGAGAA 
GCAGACAAAC AGAAAGCCAA AGGCAAAAAA AAGCAGTGGT GGCGGCCTTG GGTCGATCAT 
GCTTCCATGG TCAGGAGTGG AGATTATTAT TTGTTTGAAA CGGATAGTGA AGAAGAGGAA 
GAGGAAGAAT TTAAAAAGGA AGAAGAAGAG CCTCCAAGAA GGTCGGCCTT CCAGTTTGTT 
TATCAAGCCT GGATTACTGA CCCTAAAACA GCACTCCGTC AGAGGCGCAA AGAGAAAAAG 
AGATCTTCAA GAGAAGGCCA GAAACGAAGG AGAAAGGGAT CTGGGGAGGG TCCTGTGGAG 
TGGGAAGATC GAGAGGATGA ACCAGTCAAA AAGAAATCTG ATGGCCCAGA TAATATCATC 
AAGAGGATAT TTAATATTTT GAAATTTACC TGGGTCCTGT TTCTGGCAAC GGTGGATAGT 
TTCACAACTT GGCTTAATTC CATTTCAAGG GAGCATATTG ATATATCCAC AGTTCTGAGA 
ATTGAAAGAT GCATGCTGAC CAGAGAAATT AAAAAGGGCA ACGTTCCCAC TCGGGAGAGC 
ATCCACATGT ACTATCAGAA CCACATCATG AACCTTTCCC GAGAGTCTGG ACTGGACACC 
ATCGACGACC GTCCTGGTGC TGCTTCAGGT GCACAGACAG CCCACAGGAT GGATAGCTTA 
GATTCTCATG ACAGTATCTC CAGCGAGCCC ACCCAGTGCA CCATTCTGTC CTCGCGCCAG 
GGGACCACGG AAACCATCGA GGAGGTGGAG ACTGAGCAGG ACGAGGAGGC CGAGGGGGAC 
GAGGAGGCCG GGGAGGCTGA GGAGGCGGGG GTCGGCGACC CTGACGAGGC GGGTTTGGAG 
GCCCCAGAGG ATGGCGAGGA TGGCGTCGAG GAGGCCCCGC CGTCGTACAG CAAGGCCGTC 
AGCCTGGAGC GCCTGTCCTT GGACTCGCCG GACGGCTCCG CAGGCCGGAA CCACATGGCG 
GTCAGCCCCG ACGACAGCCG CTCCGACAGG CTGGAGTCCA GCATCTTACC CCCGCTGACT 
CACGAGCTGA CGGCCAGCGA GCTGCTGCTG AACAAGATGT TCCATGACGA TGAGCTGGAG 
GAGTCGGAGA AATTCTACGT TGGCCAGCCG CGGTTCTTGC TGCTCTTCTA CGCCATGTAC 
AACACCCTGG TGGCCCGCTC GGAAATGGTG TGCTATTTCG TCATCATCCT CAACCACATG 
GTCTCTGCCT CCATGATCAC CCTCGTGCTT CCCATCCTGA TCTTCCTCTG GGCCATGCTG 
TCTGTCCCCA GGCCCAGCAA GCGGTTCTGG ATGATGGCCA TTGTCTACAC AGAGGTGGCA 
ATTGTGGTCA AGTATTTCTT CCAGTTTGGG TTCTTTCCCT GGAACAAGCA CGTGGAATTG 
AACAAAGACA AACCTTACCA CCCGCCTAAT ATCATAGGAG TGGAAAAGAA AGAAGGTTAT 
GTCCTTTACG ACCTCATTCA GCTCCTGGCT CTGTTCTTCC ATCGATCGAT TCTGAAGTGC 
CACGGTTTAT GGGATGAAGA TGACGTCACC GACAGTGGCT TAGACAAGGA AGAGTCAGAT 
GACGAGCTCT CCCTCAGTCA CCGCAGAAGA GCTTCCTCCG ATTCTCTGAA ATCCATCAAC 
CTGGCCGCAT CGGCGGAGTC CGTGCACGTG TCCTTCCCCG AGCAGCCGGC AGCCGTCCGC 
AGGAAGCGCT CCGCCAGCAG CTCCCAGATA TCCCAGAGAT CCAGCTTTTC TTCAAACAGG 
TCCAAAAGAG GCAGCACAAG CACCCGAAAC AGCAGTCAAA AAGGAAGCAG TGTTTTGAGC 
ATTAAGCAAA AAAGCAAAAG GGAGCTTTAT ATGGAAAAGC TTCAAGAACA TTTAATCAAA 
GCAAAAGCAT TTACTATAAA GAAGACTCTG CAAATATATG TGCCCATCAG ACAGTTCTTC 
TACAACCTCA TCCACCCCGA CTACAGCGCC GTGACCGATG TGTATGTGCT CATGTTCCTG 
GCCGACACTG TCGACTTTGT CATCATTGTC TTTGGCTTCT GGGCCTTTGG GAAACACTCA 
GCAGCTGCAG ACATCACCTC TTCGCTGTCC GAGGACCAGG TCCCTGGGCC GTTTTTGGTG 
ATGGTCCTCA TCCAGTTTGG AACCATGGTG GTGGACCGAG CACTGTACCT CAGGAAGACT 
GTATTGGGAA AGGTCATTTT CCAGGTCATT CTTGTGTTTG GGATTCACTT CTGGATGTTC 
TTCATCTTGC CTGGCGTGAC GGAGAGGAAA TTCAGCCAGA ACCTGGTTGC TCAGCTTTGG 
TACTTTGTGA AGTGTGTTTA CTTCGGGCTG TCTGCCTACC AAATCCGTTG TGGCTACCCA 
ACTCGTGTCC TTGGAAACTT CCTCACAAAG AGCTACAATT ATGTCAACCT CTTCTTGTTT 
CAAGGGTTCC GCCTTGTTCC GTTTTTGACT GAGCTGAGGG CCGTGATGGA CTGGGTGTGG 
ACAGACACGA CCTTGAGCCT CTCCAGCTGG ATCTGTGTAG AGGACATCTA TGCTCACATA 
TTCATCCTGA AGTGTTGGCG GGAGTCAGAA AAAAGATACC CTCAGCCTCG GGGGCAGAAA 
AAGAAGAAGG TGGTAAAGTA TGGCATGGGG GGCATGATCA TCGTGCTGCT CATCTGCATC 
GTCTGGTTTC CTCTTCTCTT CATGTCCCTG ATCAAGTCTG TCGCTGGAGT GATCAACCAG 
CCCCTGGATG TCTCCGTCAC AATCACCCTG GGAGGGTACC AGCCTATTTT CACGATGAGT 
GCCCAGCAAA GCCAGTTGAA AGTTATGGAC CAGCCAAAGT TTAATAAATT TATAAAAGCT 
TTTTCTAGGG ACACTGGTGC TATGCAATTT CTGGAAAATT ATGAAAAAGA AGACATAACA 
GTAGCAGAAC TGGAAGGAAA CTCAAATTCT TTGTGGACCA TCAGCCCTCC CAAAACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023503241.1
CDS	40..7539
Translation	MASEVVCGLIFRLLLPICLAVACAFRYNGLSFVYLIYLLLIPLFSEPTKATMQGHTGRLLKSLCFTSLSFLLLHVIFHITLASLEAQHRIAPGYNCSTWEKTLRQIGFESLKGADAGNGIRVFVPDIGMFIASLTIWLVCRNIVQKPVTEEAAQCNPEFQNEEVAGGEKIDPEEALIYEEDLDEGDGVEGELEDSTKLKMFRRLASVASKLKEFIGNMITTAGKVVVTILLGSSGMMLPSLTSSVYFFVFLGLCTWWSWCRTFDPLLFSCLCVLLAIFTAGHLIGLYLYQFQFFQEAVPPNDYYARLFGIKSVIQTDCSSTWKIIANPDLSWYHHANPILLLVMYYTLATLIRIWLQEPLVQDERTKEEDRALACSPIQITAERRRSLWYATHYPTDERKLLSMTQDDYKPSDGLLVTVNGNPVDYHTIHPSLPMENGPAKADLYSTPQYRWEPSDDSSEKKEEEEDEKEEFEEERSHEEKRSVKVHAMVSVFQFIMKQSYICALIAMMAWSITYHSWLTFVLLIWSCTLWMIRNRRKYAMISSPFMVVYGNLLLILQYIWSFELPEIKKVPGFLEKKEPGELASKILFTITFWLLLRQHLTEQKALREKEALLSEVKIGSQENEEKADEQDIQVEGEAEEKEEGEEAKREKQEIKKEEEEEEEVEEGDDQDIMTVLGNLVVAMFIKYWIYVCGGMFFFVSFEGKIVMYKIIYMVLFLFCVALYQVHYEWWRRILKYFWMSVVIYTMLVLIFIYTYQFENFPGLWQNMTGLKREKLEDLGLKQFTVAELFTRIFIPTSFLLVCILHLHYFHDRFLELTDLKSIPCKEDSAIYRLAHPEGSLPDLTMMNLTASLQAPEMGKPAEPGAEKLEGCPEKAEKAELGKDSEEEEEEEEEEEEAGTSDLRNKWHLVIDRLTVLFLKFLEYFHKLQVFMWWILELHIIKIVSSYIIWVSVKEVSLFNYVFLISWAFALPYAKLRRLASSVCTVWTCVIIVCKMLYQLQTIKPENFSVNCSLPNENQTNVPLQQLNKSLLYNAPIDPTEWVGLRKSSPLLVYLRNNLLMLAILAFEVTIYRHQEYYRGRNNLTAPVSKTIFHDITRLHLDDGLINCAKYFINYFFYKFGLETCFLMSVNVIGQRMDFYAMIHACWLIAVLYRRRRKAIAEIWPKYCCFLACIITFQYFICIGIPPAPCRDYPWRFKGASFNDNIIKWLYFPDFIVRPNPVFLVYDFMLLLCASLQRQIFEDENKAAVRIMAGDNVEICMNLDAASFSQHNPVPDFIHCRSYLDMSKVIIFSYLFWFVLTIIFITGTTRISIFCMGYLVACFYFLLFGGNLLLKPIKSILRYWDWLIAYNVFVITMKNILSIGACGYIEKLVQNSCWLIQAFSLACTVKGYKMPDNDSSCKLPSGEAGIIWDSICFAFLLLQRRVFMSYYFLHVVADIKASQILASRGAELFQATIVKAVKARIEEEKKSMDQLKRQMDRIKARQQKYKKGKERMLSLTQESGEGQDIQKLSEEDDEREADKQKAKGKKKQWWRPWVDHASMVRSGDYYLFETDSEEEEEEEFKKEEEEPPRRSAFQFVYQAWITDPKTALRQRRKEKKRSSREGQKRRRKGSGEGPVEWEDREDEPVKKKSDGPDNIIKRIFNILKFTWVLFLATVDSFTTWLNSISREHIDISTVLRIERCMLTREIKKGNVPTRESIHMYYQNHIMNLSRESGLDTIDDRPGAASGAQTAHRMDSLDSHDSISSEPTQCTILSSRQGTTETIEEVETEQDEEAEGDEEAGEAEEAGVGDPDEAGLEAPEDGEDGVEEAPPSYSKAVSLERLSLDSPDGSAGRNHMAVSPDDSRSDRLESSILPPLTHELTASELLLNKMFHDDELEESEKFYVGQPRFLLLFYAMYNTLVARSEMVCYFVIILNHMVSASMITLVLPILIFLWAMLSVPRPSKRFWMMAIVYTEVAIVVKYFFQFGFFPWNKHVELNKDKPYHPPNIIGVEKKEGYVLYDLIQLLALFFHRSILKCHGLWDEDDVTDSGLDKEESDDELSLSHRRRASSDSLKSINLAASAESVHVSFPEQPAAVRRKRSASSSQISQRSSFSSNRSKRGSTSTRNSSQKGSSVLSIKQKSKRELYMEKLQEHLIKAKAFTIKKTLQIYVPIRQFFYNLIHPDYSAVTDVYVLMFLADTVDFVIIVFGFWAFGKHSAAADITSSLSEDQVPGPFLVMVLIQFGTMVVDRALYLRKTVLGKVIFQVILVFGIHFWMFFILPGVTERKFSQNLVAQLWYFVKCVYFGLSAYQIRCGYPTRVLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFILKCWRESEKRYPQPRGQKKKKVVKYGMGGMIIVLLICIVWFPLLFMSLIKSVAGVINQPLDVSVTITLGGYQPIFTMSAQQSQLKVMDQPKFNKFIKAFSRDTGAMQFLENYEKEDITVAELEGNSNSLWTISPPKT

Target ORF information:

RefSeq Version	XM_023647473.1
Organism	Equus caballus(horse)
Definition	Equus caballus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023647473.1

ORF Insert Sequence:

ATGGCCTCGG AAGTGGTGTG CGGACTCATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
GTAGCGTGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTCATCTA CCTCTTGCTC 
ATCCCTCTGT TCTCAGAACC AACAAAAGCG ACGATGCAAG GACATACGGG ACGGTTGTTG 
AAGTCTCTGT GCTTCACCAG TCTGTCCTTC CTGTTGCTGC ACGTCATCTT TCACATCACA 
TTGGCCAGCC TGGAAGCTCA GCACCGTATC GCGCCTGGTT ACAACTGCTC GACGTGGGAA 
AAGACGCTTC GGCAGATTGG CTTTGAAAGC TTAAAGGGAG CCGACGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCGTCTGT 
AGGAACATTG TTCAGAAACC GGTGACAGAA GAAGCCGCAC AGTGTAACCC GGAGTTTCAA 
AATGAAGAAG TGGCTGGAGG AGAAAAAATA GATCCAGAAG AGGCACTGAT CTACGAAGAG 
GATTTAGATG AAGGAGATGG TGTTGAAGGA GAGTTGGAAG ACAGCACAAA ATTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCTTCCAAG CTTAAGGAGT TTATTGGCAA CATGATAACC 
ACCGCTGGAA AAGTTGTTGT GACAATTTTA CTGGGGTCAT CAGGTATGAT GCTGCCGTCT 
CTGACCTCGT CTGTGTACTT CTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACAGCA 
GGGCACTTAA TTGGACTTTA TTTATATCAG TTCCAATTCT TTCAAGAAGC GGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAATCAGTCA TTCAAACAGA CTGTTCCAGC 
ACGTGGAAGA TCATAGCGAA CCCAGACCTG TCCTGGTATC ACCACGCCAA CCCCATCCTC 
CTGCTGGTGA TGTACTACAC GCTGGCCACT CTCATCCGCA TCTGGCTACA GGAGCCGCTT 
GTGCAGGATG AGAGGACCAA GGAAGAGGAC AGAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACGGCCGAGA GGAGGCGGAG CCTGTGGTAC GCGACCCATT ACCCAACTGA CGAGAGAAAA 
CTTCTATCCA TGACTCAAGA TGACTACAAA CCATCTGATG GTCTGCTGGT GACTGTGAAC 
GGCAATCCTG TGGATTACCA CACCATCCAC CCCAGTCTTC CCATGGAGAA CGGCCCCGCC 
AAAGCCGACC TGTACTCCAC CCCGCAGTAC CGGTGGGAGC CCTCCGACGA CTCCTCGGAA 
AAGAAAGAGG AAGAAGAAGA TGAGAAAGAA GAATTTGAAG AGGAAAGGAG CCATGAGGAG 
AAAAGAAGTG TTAAAGTTCA CGCCATGGTC TCTGTGTTCC AGTTTATTAT GAAACAAAGT 
TACATCTGTG CCCTTATTGC TATGATGGCT TGGAGCATCA CCTATCACAG CTGGTTGACC 
TTCGTGCTGT TGATTTGGTC GTGCACTCTT TGGATGATTC GCAACAGGAG AAAATATGCC 
ATGATCAGCT CTCCTTTCAT GGTTGTTTAT GGAAATCTAC TGCTGATATT ACAGTATATA 
TGGAGTTTTG AACTTCCTGA AATTAAAAAG GTCCCAGGAT TTTTAGAAAA GAAAGAGCCA 
GGAGAACTTG CCTCAAAGAT ACTTTTCACC ATTACTTTTT GGCTACTGCT GAGGCAGCAC 
CTCACAGAGC AGAAAGCTCT TCGGGAAAAG GAAGCTCTTT TATCTGAGGT CAAAATTGGG 
AGTCAGGAAA ATGAAGAAAA AGCTGATGAG CAAGATATAC AGGTGGAAGG AGAGGCGGAA 
GAGAAGGAGG AAGGAGAGGA AGCAAAGCGA GAGAAGCAGG AGATAAAGAA GGAAGAGGAA 
GAGGAAGAGG AAGTGGAGGA AGGTGACGAT CAGGACATCA TGACGGTGCT GGGCAATCTG 
GTGGTGGCCA TGTTCATTAA GTACTGGATC TACGTCTGTG GAGGGATGTT CTTCTTCGTC 
AGCTTCGAGG GTAAAATTGT GATGTACAAA ATCATCTACA TGGTGCTGTT TCTATTCTGT 
GTGGCCTTGT ATCAGGTGCA CTATGAATGG TGGAGGCGAA TTCTAAAATA TTTTTGGATG 
TCAGTGGTTA TTTATACTAT GCTGGTGCTC ATCTTTATAT ACACATATCA GTTTGAAAAC 
TTCCCAGGCC TATGGCAAAA TATGACTGGA TTAAAAAGAG AAAAGCTTGA GGATCTTGGC 
TTAAAGCAGT TTACCGTGGC TGAACTATTC ACTCGCATAT TCATCCCAAC CTCCTTTCTC 
CTGGTGTGCA TTTTGCACCT TCACTATTTC CATGACCGGT TCCTGGAGCT CACAGACCTG 
AAGTCCATTC CCTGCAAGGA AGACAGCGCC ATCTACAGAC TGGCGCACCC TGAGGGAAGC 
CTGCCGGACC TCACCATGAT GAACCTGACC GCCAGCCTGC AGGCGCCGGA GATGGGGAAG 
CCGGCAGAGC CTGGTGCGGA GAAGCTGGAG GGGTGCCCTG AGAAAGCCGA GAAGGCTGAG 
CTTGGGAAAG ACAGTGAGGA GGAGGAGGAG GAGGAAGAGG AAGAGGAGGA GGCAGGGACG 
TCAGATTTAA GGAACAAATG GCACCTGGTG ATTGACCGCC TTACTGTGCT CTTCTTAAAA 
TTCCTGGAGT ATTTTCACAA GCTGCAGGTG TTCATGTGGT GGATTTTGGA GTTGCATATC 
ATCAAAATCG TTTCATCATA CATTATCTGG GTTTCTGTGA AAGAGGTGTC TCTGTTCAAC 
TATGTATTTT TGATTTCTTG GGCTTTTGCT CTGCCATATG CCAAGCTACG CCGTCTGGCT 
TCAAGTGTCT GCACTGTCTG GACGTGTGTG ATCATCGTCT GCAAAATGTT GTACCAGCTG 
CAAACCATTA AGCCTGAGAA CTTTTCTGTT AACTGTTCCT TGCCAAATGA AAACCAAACG 
AATGTCCCCC TCCAGCAGTT GAACAAGTCA CTGCTCTACA ACGCTCCTAT TGACCCGACA 
GAGTGGGTCG GCCTGAGGAA GTCCTCCCCT TTGCTGGTCT ACCTGAGGAA TAACCTCCTG 
ATGCTGGCCA TCCTGGCCTT CGAGGTTACA ATTTACCGCC ATCAGGAATA CTATCGAGGT 
CGGAACAATC TCACGGCCCC CGTGTCTAAA ACCATCTTTC ACGACATTAC GAGGCTCCAT 
CTAGATGATG GACTTATTAA TTGTGCCAAG TATTTCATAA ATTACTTCTT CTACAAGTTT 
GGCCTAGAGA CCTGTTTCCT AATGTCAGTG AACGTCATTG GGCAGCGAAT GGACTTCTAT 
GCCATGATTC ACGCCTGCTG GTTGATCGCT GTCTTATATC GACGCAGAAG AAAAGCCATT 
GCGGAGATCT GGCCCAAGTA CTGCTGCTTC CTGGCCTGCA TCATCACGTT CCAGTACTTC 
ATCTGCATCG GCATTCCTCC TGCTCCTTGC AGAGATTACC CGTGGAGATT CAAGGGTGCC 
AGCTTCAACG ACAACATCAT TAAGTGGCTG TACTTCCCAG ACTTCATTGT GCGGCCCAAC 
CCCGTGTTTC TCGTCTATGA CTTCATGCTG CTTCTGTGTG CCTCCTTACA AAGGCAAATT 
TTTGAGGATG AAAATAAGGC TGCAGTGCGA ATCATGGCGG GAGACAATGT CGAGATTTGC 
ATGAACCTTG ATGCAGCCTC TTTCAGCCAG CATAACCCTG TACCAGACTT CATTCACTGC 
AGGTCGTACT TAGACATGTC CAAAGTAATC ATCTTCAGCT ACCTCTTCTG GTTCGTCCTC 
ACGATCATCT TCATCACTGG GACCACCAGG ATCAGCATCT TTTGCATGGG TTACTTGGTG 
GCCTGCTTCT ATTTCCTGCT TTTTGGGGGC AATTTGCTGT TGAAACCCAT CAAGAGCATC 
CTGCGCTATT GGGACTGGCT GATTGCATAC AACGTTTTTG TGATTACAAT GAAAAACATA 
CTGTCAATAG GAGCATGTGG ATACATTGAA AAATTGGTTC AAAATAGTTG CTGGTTGATC 
CAAGCTTTCA GCCTGGCCTG CACCGTCAAA GGCTATAAAA TGCCTGATAA CGATTCCTCA 
TGTAAACTGC CCAGTGGTGA AGCAGGAATT ATCTGGGACA GCATCTGTTT TGCCTTCCTC 
CTGCTGCAGA GGAGAGTTTT CATGAGTTAC TATTTTCTAC ATGTCGTGGC AGATATAAAA 
GCTTCACAGA TCCTGGCATC AAGAGGGGCA GAACTTTTCC AGGCCACAAT TGTGAAGGCT 
GTAAAGGCCA GAATTGAGGA GGAGAAGAAG TCAATGGATC AGTTGAAGAG GCAGATGGAT 
CGCATCAAGG CCAGACAACA GAAATACAAA AAGGGTAAGG AGAGGATGCT GAGCTTGACT 
CAGGAGTCAG GGGAAGGCCA AGACATCCAA AAGCTCTCTG AAGAGGATGA TGAAAGAGAA 
GCAGACAAAC AGAAAGCCAA AGGCAAAAAA AAGCAGTGGT GGCGGCCTTG GGTCGATCAT 
GCTTCCATGG TCAGGAGTGG AGATTATTAT TTGTTTGAAA CGGATAGTGA AGAAGAGGAA 
GAGGAAGAAT TTAAAAAGGA AGAAGAAGAG CCTCCAAGAA GGTCGGCCTT CCAGTTTGTT 
TATCAAGCCT GGATTACTGA CCCTAAAACA GCACTCCGTC AGAGGCGCAA AGAGAAAAAG 
AGATCTTCAA GAGAAGGCCA GAAACGAAGG AGAAAGGGAT CTGGGGAGGG TCCTGTGGAG 
TGGGAAGATC GAGAGGATGA ACCAGTCAAA AAGAAATCTG ATGGCCCAGA TAATATCATC 
AAGAGGATAT TTAATATTTT GAAATTTACC TGGGTCCTGT TTCTGGCAAC GGTGGATAGT 
TTCACAACTT GGCTTAATTC CATTTCAAGG GAGCATATTG ATATATCCAC AGTTCTGAGA 
ATTGAAAGAT GCATGCTGAC CAGAGAAATT AAAAAGGGCA ACGTTCCCAC TCGGGAGAGC 
ATCCACATGT ACTATCAGAA CCACATCATG AACCTTTCCC GAGAGTCTGG ACTGGACACC 
ATCGACGACC GTCCTGGTGC TGCTTCAGGT GCACAGACAG CCCACAGGAT GGATAGCTTA 
GATTCTCATG ACAGTATCTC CAGCGAGCCC ACCCAGTGCA CCATTCTGTC CTCGCGCCAG 
GGGACCACGG AAACCATCGA GGAGGTGGAG ACTGAGCAGG ACGAGGAGGC CGAGGGGGAC 
GAGGAGGCCG GGGAGGCTGA GGAGGCGGGG GTCGGCGACC CTGACGAGGC GGGTTTGGAG 
GCCCCAGAGG ATGGCGAGGA TGGCGTCGAG GAGGCCCCGC CGTCGTACAG CAAGGCCGTC 
AGCCTGGAGC GCCTGTCCTT GGACTCGCCG GACGGCTCCG CAGGCCGGAA CCACATGGCG 
GTCAGCCCCG ACGACAGCCG CTCCGACAGG CTGGAGTCCA GCATCTTACC CCCGCTGACT 
CACGAGCTGA CGGCCAGCGA GCTGCTGCTG AACAAGATGT TCCATGACGA TGAGCTGGAG 
GAGTCGGAGA AATTCTACGT TGGCCAGCCG CGGTTCTTGC TGCTCTTCTA CGCCATGTAC 
AACACCCTGG TGGCCCGCTC GGAAATGGTG TGCTATTTCG TCATCATCCT CAACCACATG 
GTCTCTGCCT CCATGATCAC CCTCGTGCTT CCCATCCTGA TCTTCCTCTG GGCCATGCTG 
TCTGTCCCCA GGCCCAGCAA GCGGTTCTGG ATGATGGCCA TTGTCTACAC AGAGGTGGCA 
ATTGTGGTCA AGTATTTCTT CCAGTTTGGG TTCTTTCCCT GGAACAAGCA CGTGGAATTG 
AACAAAGACA AACCTTACCA CCCGCCTAAT ATCATAGGAG TGGAAAAGAA AGAAGGTTAT 
GTCCTTTACG ACCTCATTCA GCTCCTGGCT CTGTTCTTCC ATCGATCGAT TCTGAAGTGC 
CACGGTTTAT GGGATGAAGA TGACGTCACC GACAGTGGCT TAGACAAGGA AGAGTCAGAT 
GACGAGCTCT CCCTCAGTCA CCGCAGAAGA GCTTCCTCCG ATTCTCTGAA ATCCATCAAC 
CTGGCCGCAT CGGCGGAGTC CGTGCACGTG TCCTTCCCCG AGCAGCCGGC AGCCGTCCGC 
AGGAAGCGCT CCGCCAGCAG CTCCCAGATA TCCCAGAGAT CCAGCTTTTC TTCAAACAGG 
TCCAAAAGAG GCAGCACAAG CACCCGAAAC AGCAGTCAAA AAGGAAGCAG TGTTTTGAGC 
ATTAAGCAAA AAAGCAAAAG GGAGCTTTAT ATGGAAAAGC TTCAAGAACA TTTAATCAAA 
GCAAAAGCAT TTACTATAAA GAAGACTCTG CAAATATATG TGCCCATCAG ACAGTTCTTC 
TACAACCTCA TCCACCCCGA CTACAGCGCC GTGACCGATG TGTATGTGCT CATGTTCCTG 
GCCGACACTG TCGACTTTGT CATCATTGTC TTTGGCTTCT GGGCCTTTGG GAAACACTCA 
GCAGCTGCAG ACATCACCTC TTCGCTGTCC GAGGACCAGG TCCCTGGGCC GTTTTTGGTG 
ATGGTCCTCA TCCAGTTTGG AACCATGGTG GTGGACCGAG CACTGTACCT CAGGAAGACT 
GTATTGGGAA AGGTCATTTT CCAGGTCATT CTTGTGTTTG GGATTCACTT CTGGATGTTC 
TTCATCTTGC CTGGCGTGAC GGAGAGGAAA TTCAGCCAGA ACCTGGTTGC TCAGCTTTGG 
TACTTTGTGA AGTGTGTTTA CTTCGGGCTG TCTGCCTACC AAATCCGTTG TGGCTACCCA 
ACTCGTGTCC TTGGAAACTT CCTCACAAAG AGCTACAATT ATGTCAACCT CTTCTTGTTT 
CAAGGGTTCC GCCTTGTTCC GTTTTTGACT GAGCTGAGGG CCGTGATGGA CTGGGTGTGG 
ACAGACACGA CCTTGAGCCT CTCCAGCTGG ATCTGTGTAG AGGACATCTA TGCTCACATA 
TTCATCCTGA AGTGTTGGCG GGAGTCAGAA AAAAGATACC CTCAGCCTCG GGGGCAGAAA 
AAGAAGAAGG TGGTAAAGTA TGGCATGGGG GGCATGATCA TCGTGCTGCT CATCTGCATC 
GTCTGGTTTC CTCTTCTCTT CATGTCCCTG ATCAAGTCTG TCGCTGGAGT GATCAACCAG 
CCCCTGGATG TCTCCGTCAC AATCACCCTG GGAGGGTACC AGCCTATTTT CACGATGAGT 
GCCCAGCAAA GCCAGTTGAA AGTTATGGAC CAGCCAAAGT TTAATAAATT TATAAAAGCT 
TTTTCTAGGG ACACTGGTGC TATGCAATTT CTGGAAAATT ATGAAAAAGA AGACATAACA 
GTAGCAGAAC TGGAAGGAAA CTCAAATTCT TTGTGGACCA TCAGCCCTCC CAAAACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIEZO2 ( XM_014727615.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_014583101.1 Equus caballus piezo-type mechanosensitive ion channel component 2 (PIEZO2), mRNA.

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CloneID	OHb32427
Clone ID Related Accession (Same CDS sequence)	XM_014727615.1
Accession Version	XM_014727615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1447862400000
Organism	Equus caballus(horse)
Product	piezo-type mechanosensitive ion channel component 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867430.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGGAACCT GGAGAGCGTG TGCATTCCGA TACAATGGGC TCTCCTTTGT CTACCTCATC 
TACCTCTTGC TCATCCCTCT GTTCTCAGAA CCAACAAAAG CGACGATGCA AGGACATACG 
GGACGGTTGT TGAAGTCTCT GTGCTTCACC AGTCTGTCCT TCCTGTTGCT GCACGTCATC 
TTTCACATCA CATTGGCCAG CCTGGAAGCT CAGCACCGTA TCGCGCCTGG TTACAACTGC 
TCGACGTGGG AAAAGACGCT TCGGCAGATT GGCTTTGAAA GCTTAAAGGG AGCCGACGCT 
GGCAATGGGA TCAGAGTGTT TGTACCTGAC ATCGGGATGT TCATTGCTAG TCTGACCATC 
TGGCTCGTCT GTAGGAACAT TGTTCAGAAA CCGGTGACAG AAGAAGCCGC ACAGTGTAAC 
CCGGAGTTTC AAAATGAAGA AGTGGCTGGA GGAGAAAAAA TAGATCCAGA AGAGGCACTG 
ATCTACGAAG AGGATTTAGA TGAAGGAGAT GGTGTTGAAG GAGAGTTGGA AGACAGCACA 
AAATTAAAAA TGTTCCGCAG GCTTGCCTCT GTGGCTTCCA AGCTTAAGGA GTTTATTGGC 
AACATGATAA CCACCGCTGG AAAAGTTGTT GTGACAATTT TACTGGGGTC ATCAGGTATG 
ATGCTGCCGT CTCTGACCTC GTCTGTGTAC TTCTTTGTAT TTTTGGGTCT GTGCACCTGG 
TGGTCCTGGT GCCGGACGTT CGACCCATTG CTGTTCAGCT GTCTCTGTGT TCTGCTGGCT 
ATTTTCACAG CAGGGCACTT AATTGGACTT TATTTATATC AGTTCCAATT CTTTCAAGAA 
GCGGTTCCAC CCAATGACTA CTATGCAAGG TTGTTTGGTA TCAAATCAGT CATTCAAACA 
GACTGTTCCA GCACGTGGAA GATCATAGCG AACCCAGACC TGTCCTGGTA TCACCACGCC 
AACCCCATCC TCCTGCTGGT GATGTACTAC ACGCTGGCCA CTCTCATCCG CATCTGGCTA 
CAGGAGCCGC TTGTGCAGGA TGAGAGGACC AAGGAAGAGG ACAGAGCCCT GGCTTGTAGC 
CCCATCCAAA TAACGGCCGA GAGGAGGCGG AGCCTGTGGT ACGCGACCCA TTACCCAACT 
GACGAGAGAA AACTTCTATC CATGACTCAA GATGACTACA AACCATCTGA TGGTCTGCTG 
GTGACTGTGA ACGGCAATCC TGTGGATTAC CACACCATCC ACCCCAGTCT TCCCATGGAG 
AACGGCCCCG CCAAAGCCGA CCTGTACTCC ACCCCGCAGT ACCGGTGGGA GCCCTCCGAC 
GACTCCTCGG AAAAGAAAGA GGAAGAAGAA GATGAGAAAG AAGAATTTGA AGAGGAAAGG 
AGCCATGAGG AGAAAAGAAG TGTTAAAGTT CACGCCATGG TCTCTGTGTT CCAGTTTATT 
ATGAAACAAA GTTACATCTG TGCCCTTATT GCTATGATGG CTTGGAGCAT CACCTATCAC 
AGCTGGTTGA CCTTCGTGCT GTTGATTTGG TCGTGCACTC TTTGGATGAT TCGCAACAGG 
AGAAAATATG CCATGATCAG CTCTCCTTTC ATGGTTGTTT ATGGAAATCT ACTGCTGATA 
TTACAGTATA TATGGAGTTT TGAACTTCCT GAAATTAAAA AGGTCCCAGG ATTTTTAGAA 
AAGAAAGAGC CAGGAGAACT TGCCTCAAAG ATACTTTTCA CCATTACTTT TTGGCTACTG 
CTGAGGCAGC ACCTCACAGA GCAGAAAGCT CTTCGGGAAA AGGAAGCTCT TTTATCTGAG 
GTCAAAATTG GGAGTCAGGA AAATGAAGAA AAAGCTGATG AGCAAGATAT ACAGGTGGAA 
GGAGAGGCGG AAGAGAAGGA GGAAGGAGAG GAAGCAAAGC GAGAGAAGCA GGAGATAAAG 
AAGGAAGAGG AAGAGGAAGA GGAAGTGGAG GAAGGTGACG ATCAGGACAT CATGACGGTG 
CTGGGCAATC TGGTGGTGGC CATGTTCATT AAGTACTGGA TCTACGTCTG TGGAGGGATG 
TTCTTCTTCG TCAGCTTCGA GGGTAAAATT GTGATGTACA AAATCATCTA CATGGTGCTG 
TTTCTATTCT GTGTGGCCTT GTATCAGGTG CACTATGAAT GGTGGAGGCG AATTCTAAAA 
TATTTTTGGA TGTCAGTGGT TATTTATACT ATGCTGGTGC TCATCTTTAT ATACACATAT 
CAGTTTGAAA ACTTCCCAGG CCTATGGCAA AATATGACTG GATTAAAAAG AGAAAAGCTT 
GAGGATCTTG GCTTAAAGCA GTTTACCGTG GCTGAACTAT TCACTCGCAT ATTCATCCCA 
ACCTCCTTTC TCCTGGTGTG CATTTTGCAC CTTCACTATT TCCATGACCG GTTCCTGGAG 
CTCACAGACC TGAAGTCCAT TCCCTGCAAG GAAGACAGCG CCATCTACAG ACTGGCGCAC 
CCTGAGGGAA GCCTGCCGGA CCTCACCATG ATGAACCTGA CCGCCAGCCT GCAGGCGCCG 
GAGATGGGGA AGCCGGCAGA GCCTGGTGCG GAGAAGCTGG AGGGGTGCCC TGAGAAAGCC 
GAGAAGGCTG AGCTTGGGAA AGACAGTGAG GAGGAGGAGG AGGAGGAAGA GGAAGAGGAG 
GAGGCAGGGA CGTCAGATTT AAGGAACAAA TGGCACCTGG TGATTGACCG CCTTACTGTG 
CTCTTCTTAA AATTCCTGGA GTATTTTCAC AAGCTGCAGG TGTTCATGTG GTGGATTTTG 
GAGTTGCATA TCATCAAAAT CGTTTCATCA TACATTATCT GGGTTTCTGT GAAAGAGGTG 
TCTCTGTTCA ACTATGTATT TTTGATTTCT TGGGCTTTTG CTCTGCCATA TGCCAAGCTA 
CGCCGTCTGG CTTCAAGTGT CTGCACTGTC TGGACGTGTG TGATCATCGT CTGCAAAATG 
TTGTACCAGC TGCAAACCAT TAAGCCTGAG AACTTTTCTG TTAACTGTTC CTTGCCAAAT 
GAAAACCAAA CGAATGTCCC CCTCCAGCAG TTGAACAAGT CACTGCTCTA CAACGCTCCT 
ATTGACCCGA CAGAGTGGGT CGGCCTGAGG AAGTCCTCCC CTTTGCTGGT CTACCTGAGG 
AATAACCTCC TGATGCTGGC CATCCTGGCC TTCGAGGTTA CAATTTACCG CCATCAGGAA 
TACTATCGAG GTCGGAACAA TCTCACGGCC CCCGTGTCTA AAACCATCTT TCACGACATT 
ACGAGGCTCC ATCTAGATGA TGGACTTATT AATTGTGCCA AGTATTTCAT AAATTACTTC 
TTCTACAAGT TTGGCCTAGA GACCTGTTTC CTAATGTCAG TGAACGTCAT TGGGCAGCGA 
ATGGACTTCT ATGCCATGAT TCACGCCTGC TGGTTGATCG CTGTCTTATA TCGACGCAGA 
AGAAAAGCCA TTGCGGAGAT CTGGCCCAAG TACTGCTGCT TCCTGGCCTG CATCATCACG 
TTCCAGTACT TCATCTGCAT CGGCATTCCT CCTGCTCCTT GCAGAGATTA CCCGTGGAGA 
TTCAAGGGTG CCAGCTTCAA CGACAACATC ATTAAGTGGC TGTACTTCCC AGACTTCATT 
GTGCGGCCCA ACCCCGTGTT TCTCGTCTAT GACTTCATGC TGCTTCTGTG TGCCTCCTTA 
CAAAGGCAAA TTTTTGAGGA TGAAAATAAG GCTGCAGTGC GAATCATGGC GGGAGACAAT 
GTCGAGATTT GCATGAACCT TGATGCAGCC TCTTTCAGCC AGCATAACCC TGTACCAGAC 
TTCATTCACT GCAGGTCGTA CTTAGACATG TCCAAAGTAA TCATCTTCAG CTACCTCTTC 
TGGTTCGTCC TCACGATCAT CTTCATCACT GGGACCACCA GGATCAGCAT CTTTTGCATG 
GGTTACTTGG TGGCCTGCTT CTATTTCCTG CTTTTTGGGG GCAATTTGCT GTTGAAACCC 
ATCAAGAGCA TCCTGCGCTA TTGGGACTGG CTGATTGCAT ACAACGTTTT TGTGATTACA 
ATGAAAAACA TACTGTCAAT AGGAGCATGT GGATACATTG AAAAATTGGT TCAAAATAGT 
TGCTGGTTGA TCCAAGCTTT CAGCCTGGCC TGCACCGTCA AAGGCTATAA AATGCCTGAT 
GACGATTCCT CATGTAAACT GCCCAGTGGT GAAGCAGGAA TTATCTGGGA CAGCATCTGT 
TTTGCCTTCC TCCTGCTGCA GAGGAGAGTT TTCATGAGTT ACTATTTTCT ACATGTCGTG 
GCAGATATAA AAGCTTCACA GATCCTGGCA TCAAGAGGGG CAGAACTTTT CCAGGCCACA 
ATTGTGAAGG CTGTAAAGGC CAGAATTGAG GAGGAGAAGA AGTCAATGGA TCAGTTGAAG 
AGGCAGATGG ATCGCATCAA GGCCAGACAA CAGAAATACA AAAAGGGTAA GGAGAGGATG 
CTGAGCTTGA CTCAGGAGTC AGGGGAAGGC CAAGACATCC AAAAGCTCTC TGAAGAGGAT 
GATGAAAGAG AAGCAGACAA ACAGAAAGCC AAAGGCAAAA AAAAGCAGTG GTGGCGGCCT 
TGGGTCGATC ATGCTTCCAT GGTCAGGAGT GGAGATTATT ATTTGTTTGA AACGGATAGT 
GAAGAAGAGG AAGAGGAAGA ATTTAAAAAG GAAGAAGAAG AGCCTCCAAG AAGGTCGGCC 
TTCCAGTTTG TTTATCAAGC CTGGATTACT GACCCTAAAA CAGCACTCCG TCAGAGGCGC 
AAAGAGAAAA AGAGATCTTC AAGAGAAGGC CAGAAACGAA GGAGAAAGGG ATCTGGGGAG 
GGTCCTGTGG AGTGGGAAGA TCGAGAGGAT GAACCAGTCA AAAAGAAATC TGATGGCCCA 
GATAATATCA TCAAGAGGAT ATTTAATATT TTGAAATTTA CCTGGGTCCT GTTTCTGGCA 
ACGGTGGATA GTTTCACAAC TTGGCTTAAT TCCATTTCAA GGGAGCATAT TGATATATCC 
ACAGTTCTGA GAATTGAAAG ATGCATGCTG ACCAGAGAAA TTAAAAAGGG CAACGTTCCC 
ACTCGGGAGA GCATCCACAT GTACTATCAG AACCACATCA TGAACCTTTC CCGAGAGTCT 
GGACTGGACA CCATCGACGA CCGTCCTGGT GCTGCTTCAG GTGCACAGAC AGCCCACAGG 
ATGGATAGCT TAGATTCTCA TGACAGTATC TCCAGCTGCT ACACCGAAGC AACCATGCTG 
TTCTCAAGGC AGTCTACCCT TGATGATTTA GATGGACCAG ACACTGTTCC TAAAACAAGT 
GAGCGTGCTC GACCTAGGCT CCGTAAAATG TTCAGCATGG ATGTGTCCTC CTCATCAGCT 
GACAGTGGCA GTTTAGCGTC CAGCGAGCCC ACCCAGTGCA CCATTCTGTC CTCGCGCCAG 
GGGACCACGG AAACCATCGA GGAGGTGGAG ACTGAGCAGG ACGAGGAGGC CGAGGGGGAC 
GAGGAGGCCG GGGAGGCTGA GGAGGCGGGG GTCGGCGACC CTGACGAGGC GGGTTTGGAG 
GCCCCAGAGG ATGGCGAGGA TGGCGTCGAG GAGGCCCCGC CGTCGTACAG CAAGGCCGTC 
AGCCTGGAGC GCCTGTCCTT GGACTCGCCG GACGGCTCCG CAGGCCGGAA CCACATGGCG 
GTCAGCCCCG ACGACAGCCG CTCCGACAGG CTGGAGTCCA GCATCTTACC CCCGCTGACT 
CACGAGCTGA CGGCCAGCGA GCTGCTGCTG AACAAGATGT TCCATGACGA TGAGCTGGAG 
GAGTCGGAGA AATTCTACGT TGGCCAGCCG CGGTTCTTGC TGCTCTTCTA CGCCATGTAC 
AACACCCTGG TGGCCCGCTC GGAAATGGTG TGCTATTTCG TCATCATCCT CAACCACATG 
GTCTCTGCCT CCATGATCAC CCTCGTGCTT CCCATCCTGA TCTTCCTCTG GGCCATGCTG 
TCTGTCCCCA GGCCCAGCAA GCGGTTCTGG ATGATGGCCA TTGTCTACAC AGAGGTGGCA 
ATTGTGGTCA AGTATTTCTT CCAGTTTGGG TTCTTTCCCT GGAACAAGCA CGTGGAATTG 
AACAAAGACA AACCTTACCA CCCGCCTAAT ATCATAGGAG TGGAAAAGAA AGAAGGTTAT 
GTCCTTTACG ACCTCATTCA GCTCCTGGCT CTGTTCTTCC ATCGATCGAT TCTGAAGTGC 
CACGGTTTAT GGGATGAAGA TGACGTCACC GACAGTGGCT TAGACAAGGA AGAGTCAGAT 
GACGAGCTCT CCCTCAGTCA CCGCAGAAGA GCTTCCTCCG ATTCTCTGAA ATCCATCAAC 
CTGGCCGCAT CGGCGGAGTC CGTGCACGTG TCCTTCCCCG AGCAGCCGGC AGCCGTCCGC 
AGGAAGCGCT CCGCCAGCAG CTCCCAGATA TCCCAGAGAT CCAGCTTTTC TTCAAACAGG 
TCCAAAAGAG GCAGCACAAG CACCCGAAAC AGCAGTCAAA AAGGAAGCAG TGTTTTGAGC 
ATTAAGCAAA AAAGCAAAAG GGAGCTTTAT ATGGAAAAGC TTCAAGAACA TTTAATCAAA 
GCAAAAGCAT TTACTATAAA GAAGACTCTG CAAATATATG TGCCCATCAG ACAGTTCTTC 
TACAACCTCA TCCACCCCGA CTACAGCGCC GTGACCGATG TGTATGTGCT CATGTTCCTG 
GCCGACACTG TCGACTTTGT CATCATTGTC TTTGGCTTCT GGGCCTTTGG GAAACACTCA 
GCAGCTGCAG ACATCACCTC TTCGCTGTCC GAGGACCAGG TCCCTGGGCC GTTTTTGGTG 
ATGGTCCTCA TCCAGTTTGG AACCATGGTG GTGGACCGAG CACTGTACCT CAGGAAGACT 
GTATTGGGAA AGGTCATTTT CCAGGTCATT CTTGTGTTTG GGATTCACTT CTGGATGTTC 
TTCATCTTGC CTGGCGTGAC GGAGAGGAAA TTCAGCCAGA ACCTGGTTGC TCAGCTTTGG 
TACTTTGTGA AGTGTGTTTA CTTCGGGCTG TCTGCCTACC AAATCCGTTG TGGCTACCCA 
ACTCGTGTCC TTGGAAACTT CCTCACAAAG AGCTACAATT ATGTCAACCT CTTCTTGTTT 
CAAGGGTTCC GCCTTGTTCC GTTTTTGACT GAGCTGAGGG CCGTGATGGA CTGGGTGTGG 
ACAGACACGA CCTTGAGCCT CTCCAGCTGG ATCTGTGTAG AGGACATCTA TGCTCACATA 
TTCATCCTGA AGTGTTGGCG GGAGTCAGAA AAAAGATACC CTCAGCCTCG GGGGCAGAAA 
AAGAAGAAGG TGGTAAAGTA TGGCATGGGG GGCATGATCA TCGTGCTGCT CATCTGCATC 
GTCTGGTTTC CTCTTCTCTT CATGTCCCTG ATCAAGTCTG TCGCTGGAGT GATCAACCAG 
CCCCTGGATG TCTCCGTCAC AATCACCCTG GGAGGGTACC AGCCTATTTT CACGATGAGT 
GCCCAGCAAA GCCAGTTGAA AGTTATGGAC CAGCCAAAGT TTAATAAATT TATAAAAGCT 
TTTTCTAGGG ACACTGGTGC TATGCAATTT CTGGAAAATT ATGAAAAAGA AGACATAACA 
GTAGCAGAAC TGGAAGGAAA CTCAAATTCT TTGTGGACCA TCAGCCCTCC CAGTAAGCAG 
AAAATGATAC ACGAACTCAC GGACCCCAAT AGTAGCTTCT CTGTTGTTTT TTCCTGGAGT 
ATTCAGAGAA ACATGAGTCT GGGTGCAAAA GCAGAAATAG CAACAGATAA GCTTTCCTTT 
CCTCTTAAAA ATACTACCCG AGAGAATATA GCTAAAATGA TAGCTGGCAA CAACACAGAA 
AGTTCCAAAA CACCTGTGAC TATAGAAAAG ATCTACCCGT ATTACGTGAA AGCACCCAGT 
GATTCCAACT CAAAACCTAT AAAGCAACTG TTATCTGAAA ATAATTTCAT GAACATCACC 
ATCATTTTGT CAAGAGATAC TTCAACTAAA TCTAACAGTG AGTGGTGGGT TCTCAACCTG 
ACTGGAAACA GAATATACAA CCAGCATGCT CAGGCCTTGG AGCTGGTGGT CTTCAATGAC 
AAAGTCAGCC CCCCTAGCCT GGGGTTCCTG GCTGGCTACG GTATCATGGG ACTATATGCT 
TCGGTTGTCC TCGTGATTGG GAAGTTTGTC CGTGAATTCT TCAGCGGGAT TTCTCACTCC 
ATCATGTTTG AGGAGCTTCC AAATGTCGAT CGAATTTTGA AGTTATGCAC GGATATTTTT 
TTGGTTCGAG AGACAGGGGA ACTGGAACTA GAGGAAGATC TCTATGCCAA ATTAATATTC 
CTGTATCGTT CACCAGAAAC CATGATCAAA TGGACCAGGG AAAAAACAAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014583101.1
CDS	1..8334
Translation	MGTWRACAFRYNGLSFVYLIYLLLIPLFSEPTKATMQGHTGRLLKSLCFTSLSFLLLHVIFHITLASLEAQHRIAPGYNCSTWEKTLRQIGFESLKGADAGNGIRVFVPDIGMFIASLTIWLVCRNIVQKPVTEEAAQCNPEFQNEEVAGGEKIDPEEALIYEEDLDEGDGVEGELEDSTKLKMFRRLASVASKLKEFIGNMITTAGKVVVTILLGSSGMMLPSLTSSVYFFVFLGLCTWWSWCRTFDPLLFSCLCVLLAIFTAGHLIGLYLYQFQFFQEAVPPNDYYARLFGIKSVIQTDCSSTWKIIANPDLSWYHHANPILLLVMYYTLATLIRIWLQEPLVQDERTKEEDRALACSPIQITAERRRSLWYATHYPTDERKLLSMTQDDYKPSDGLLVTVNGNPVDYHTIHPSLPMENGPAKADLYSTPQYRWEPSDDSSEKKEEEEDEKEEFEEERSHEEKRSVKVHAMVSVFQFIMKQSYICALIAMMAWSITYHSWLTFVLLIWSCTLWMIRNRRKYAMISSPFMVVYGNLLLILQYIWSFELPEIKKVPGFLEKKEPGELASKILFTITFWLLLRQHLTEQKALREKEALLSEVKIGSQENEEKADEQDIQVEGEAEEKEEGEEAKREKQEIKKEEEEEEEVEEGDDQDIMTVLGNLVVAMFIKYWIYVCGGMFFFVSFEGKIVMYKIIYMVLFLFCVALYQVHYEWWRRILKYFWMSVVIYTMLVLIFIYTYQFENFPGLWQNMTGLKREKLEDLGLKQFTVAELFTRIFIPTSFLLVCILHLHYFHDRFLELTDLKSIPCKEDSAIYRLAHPEGSLPDLTMMNLTASLQAPEMGKPAEPGAEKLEGCPEKAEKAELGKDSEEEEEEEEEEEEAGTSDLRNKWHLVIDRLTVLFLKFLEYFHKLQVFMWWILELHIIKIVSSYIIWVSVKEVSLFNYVFLISWAFALPYAKLRRLASSVCTVWTCVIIVCKMLYQLQTIKPENFSVNCSLPNENQTNVPLQQLNKSLLYNAPIDPTEWVGLRKSSPLLVYLRNNLLMLAILAFEVTIYRHQEYYRGRNNLTAPVSKTIFHDITRLHLDDGLINCAKYFINYFFYKFGLETCFLMSVNVIGQRMDFYAMIHACWLIAVLYRRRRKAIAEIWPKYCCFLACIITFQYFICIGIPPAPCRDYPWRFKGASFNDNIIKWLYFPDFIVRPNPVFLVYDFMLLLCASLQRQIFEDENKAAVRIMAGDNVEICMNLDAASFSQHNPVPDFIHCRSYLDMSKVIIFSYLFWFVLTIIFITGTTRISIFCMGYLVACFYFLLFGGNLLLKPIKSILRYWDWLIAYNVFVITMKNILSIGACGYIEKLVQNSCWLIQAFSLACTVKGYKMPDDDSSCKLPSGEAGIIWDSICFAFLLLQRRVFMSYYFLHVVADIKASQILASRGAELFQATIVKAVKARIEEEKKSMDQLKRQMDRIKARQQKYKKGKERMLSLTQESGEGQDIQKLSEEDDEREADKQKAKGKKKQWWRPWVDHASMVRSGDYYLFETDSEEEEEEEFKKEEEEPPRRSAFQFVYQAWITDPKTALRQRRKEKKRSSREGQKRRRKGSGEGPVEWEDREDEPVKKKSDGPDNIIKRIFNILKFTWVLFLATVDSFTTWLNSISREHIDISTVLRIERCMLTREIKKGNVPTRESIHMYYQNHIMNLSRESGLDTIDDRPGAASGAQTAHRMDSLDSHDSISSCYTEATMLFSRQSTLDDLDGPDTVPKTSERARPRLRKMFSMDVSSSSADSGSLASSEPTQCTILSSRQGTTETIEEVETEQDEEAEGDEEAGEAEEAGVGDPDEAGLEAPEDGEDGVEEAPPSYSKAVSLERLSLDSPDGSAGRNHMAVSPDDSRSDRLESSILPPLTHELTASELLLNKMFHDDELEESEKFYVGQPRFLLLFYAMYNTLVARSEMVCYFVIILNHMVSASMITLVLPILIFLWAMLSVPRPSKRFWMMAIVYTEVAIVVKYFFQFGFFPWNKHVELNKDKPYHPPNIIGVEKKEGYVLYDLIQLLALFFHRSILKCHGLWDEDDVTDSGLDKEESDDELSLSHRRRASSDSLKSINLAASAESVHVSFPEQPAAVRRKRSASSSQISQRSSFSSNRSKRGSTSTRNSSQKGSSVLSIKQKSKRELYMEKLQEHLIKAKAFTIKKTLQIYVPIRQFFYNLIHPDYSAVTDVYVLMFLADTVDFVIIVFGFWAFGKHSAAADITSSLSEDQVPGPFLVMVLIQFGTMVVDRALYLRKTVLGKVIFQVILVFGIHFWMFFILPGVTERKFSQNLVAQLWYFVKCVYFGLSAYQIRCGYPTRVLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFILKCWRESEKRYPQPRGQKKKKVVKYGMGGMIIVLLICIVWFPLLFMSLIKSVAGVINQPLDVSVTITLGGYQPIFTMSAQQSQLKVMDQPKFNKFIKAFSRDTGAMQFLENYEKEDITVAELEGNSNSLWTISPPSKQKMIHELTDPNSSFSVVFSWSIQRNMSLGAKAEIATDKLSFPLKNTTRENIAKMIAGNNTESSKTPVTIEKIYPYYVKAPSDSNSKPIKQLLSENNFMNITIILSRDTSTKSNSEWWVLNLTGNRIYNQHAQALELVVFNDKVSPPSLGFLAGYGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCTDIFLVRETGELELEEDLYAKLIFLYRSPETMIKWTREKTN

Target ORF information:

RefSeq Version	XM_014727615.1
Organism	Equus caballus(horse)
Definition	Equus caballus piezo-type mechanosensitive ion channel component 2 (PIEZO2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014727615.1

ORF Insert Sequence:

ATGGGAACCT GGAGAGCGTG TGCATTCCGA TACAATGGGC TCTCCTTTGT CTACCTCATC 
TACCTCTTGC TCATCCCTCT GTTCTCAGAA CCAACAAAAG CGACGATGCA AGGACATACG 
GGACGGTTGT TGAAGTCTCT GTGCTTCACC AGTCTGTCCT TCCTGTTGCT GCACGTCATC 
TTTCACATCA CATTGGCCAG CCTGGAAGCT CAGCACCGTA TCGCGCCTGG TTACAACTGC 
TCGACGTGGG AAAAGACGCT TCGGCAGATT GGCTTTGAAA GCTTAAAGGG AGCCGACGCT 
GGCAATGGGA TCAGAGTGTT TGTACCTGAC ATCGGGATGT TCATTGCTAG TCTGACCATC 
TGGCTCGTCT GTAGGAACAT TGTTCAGAAA CCGGTGACAG AAGAAGCCGC ACAGTGTAAC 
CCGGAGTTTC AAAATGAAGA AGTGGCTGGA GGAGAAAAAA TAGATCCAGA AGAGGCACTG 
ATCTACGAAG AGGATTTAGA TGAAGGAGAT GGTGTTGAAG GAGAGTTGGA AGACAGCACA 
AAATTAAAAA TGTTCCGCAG GCTTGCCTCT GTGGCTTCCA AGCTTAAGGA GTTTATTGGC 
AACATGATAA CCACCGCTGG AAAAGTTGTT GTGACAATTT TACTGGGGTC ATCAGGTATG 
ATGCTGCCGT CTCTGACCTC GTCTGTGTAC TTCTTTGTAT TTTTGGGTCT GTGCACCTGG 
TGGTCCTGGT GCCGGACGTT CGACCCATTG CTGTTCAGCT GTCTCTGTGT TCTGCTGGCT 
ATTTTCACAG CAGGGCACTT AATTGGACTT TATTTATATC AGTTCCAATT CTTTCAAGAA 
GCGGTTCCAC CCAATGACTA CTATGCAAGG TTGTTTGGTA TCAAATCAGT CATTCAAACA 
GACTGTTCCA GCACGTGGAA GATCATAGCG AACCCAGACC TGTCCTGGTA TCACCACGCC 
AACCCCATCC TCCTGCTGGT GATGTACTAC ACGCTGGCCA CTCTCATCCG CATCTGGCTA 
CAGGAGCCGC TTGTGCAGGA TGAGAGGACC AAGGAAGAGG ACAGAGCCCT GGCTTGTAGC 
CCCATCCAAA TAACGGCCGA GAGGAGGCGG AGCCTGTGGT ACGCGACCCA TTACCCAACT 
GACGAGAGAA AACTTCTATC CATGACTCAA GATGACTACA AACCATCTGA TGGTCTGCTG 
GTGACTGTGA ACGGCAATCC TGTGGATTAC CACACCATCC ACCCCAGTCT TCCCATGGAG 
AACGGCCCCG CCAAAGCCGA CCTGTACTCC ACCCCGCAGT ACCGGTGGGA GCCCTCCGAC 
GACTCCTCGG AAAAGAAAGA GGAAGAAGAA GATGAGAAAG AAGAATTTGA AGAGGAAAGG 
AGCCATGAGG AGAAAAGAAG TGTTAAAGTT CACGCCATGG TCTCTGTGTT CCAGTTTATT 
ATGAAACAAA GTTACATCTG TGCCCTTATT GCTATGATGG CTTGGAGCAT CACCTATCAC 
AGCTGGTTGA CCTTCGTGCT GTTGATTTGG TCGTGCACTC TTTGGATGAT TCGCAACAGG 
AGAAAATATG CCATGATCAG CTCTCCTTTC ATGGTTGTTT ATGGAAATCT ACTGCTGATA 
TTACAGTATA TATGGAGTTT TGAACTTCCT GAAATTAAAA AGGTCCCAGG ATTTTTAGAA 
AAGAAAGAGC CAGGAGAACT TGCCTCAAAG ATACTTTTCA CCATTACTTT TTGGCTACTG 
CTGAGGCAGC ACCTCACAGA GCAGAAAGCT CTTCGGGAAA AGGAAGCTCT TTTATCTGAG 
GTCAAAATTG GGAGTCAGGA AAATGAAGAA AAAGCTGATG AGCAAGATAT ACAGGTGGAA 
GGAGAGGCGG AAGAGAAGGA GGAAGGAGAG GAAGCAAAGC GAGAGAAGCA GGAGATAAAG 
AAGGAAGAGG AAGAGGAAGA GGAAGTGGAG GAAGGTGACG ATCAGGACAT CATGACGGTG 
CTGGGCAATC TGGTGGTGGC CATGTTCATT AAGTACTGGA TCTACGTCTG TGGAGGGATG 
TTCTTCTTCG TCAGCTTCGA GGGTAAAATT GTGATGTACA AAATCATCTA CATGGTGCTG 
TTTCTATTCT GTGTGGCCTT GTATCAGGTG CACTATGAAT GGTGGAGGCG AATTCTAAAA 
TATTTTTGGA TGTCAGTGGT TATTTATACT ATGCTGGTGC TCATCTTTAT ATACACATAT 
CAGTTTGAAA ACTTCCCAGG CCTATGGCAA AATATGACTG GATTAAAAAG AGAAAAGCTT 
GAGGATCTTG GCTTAAAGCA GTTTACCGTG GCTGAACTAT TCACTCGCAT ATTCATCCCA 
ACCTCCTTTC TCCTGGTGTG CATTTTGCAC CTTCACTATT TCCATGACCG GTTCCTGGAG 
CTCACAGACC TGAAGTCCAT TCCCTGCAAG GAAGACAGCG CCATCTACAG ACTGGCGCAC 
CCTGAGGGAA GCCTGCCGGA CCTCACCATG ATGAACCTGA CCGCCAGCCT GCAGGCGCCG 
GAGATGGGGA AGCCGGCAGA GCCTGGTGCG GAGAAGCTGG AGGGGTGCCC TGAGAAAGCC 
GAGAAGGCTG AGCTTGGGAA AGACAGTGAG GAGGAGGAGG AGGAGGAAGA GGAAGAGGAG 
GAGGCAGGGA CGTCAGATTT AAGGAACAAA TGGCACCTGG TGATTGACCG CCTTACTGTG 
CTCTTCTTAA AATTCCTGGA GTATTTTCAC AAGCTGCAGG TGTTCATGTG GTGGATTTTG 
GAGTTGCATA TCATCAAAAT CGTTTCATCA TACATTATCT GGGTTTCTGT GAAAGAGGTG 
TCTCTGTTCA ACTATGTATT TTTGATTTCT TGGGCTTTTG CTCTGCCATA TGCCAAGCTA 
CGCCGTCTGG CTTCAAGTGT CTGCACTGTC TGGACGTGTG TGATCATCGT CTGCAAAATG 
TTGTACCAGC TGCAAACCAT TAAGCCTGAG AACTTTTCTG TTAACTGTTC CTTGCCAAAT 
GAAAACCAAA CGAATGTCCC CCTCCAGCAG TTGAACAAGT CACTGCTCTA CAACGCTCCT 
ATTGACCCGA CAGAGTGGGT CGGCCTGAGG AAGTCCTCCC CTTTGCTGGT CTACCTGAGG 
AATAACCTCC TGATGCTGGC CATCCTGGCC TTCGAGGTTA CAATTTACCG CCATCAGGAA 
TACTATCGAG GTCGGAACAA TCTCACGGCC CCCGTGTCTA AAACCATCTT TCACGACATT 
ACGAGGCTCC ATCTAGATGA TGGACTTATT AATTGTGCCA AGTATTTCAT AAATTACTTC 
TTCTACAAGT TTGGCCTAGA GACCTGTTTC CTAATGTCAG TGAACGTCAT TGGGCAGCGA 
ATGGACTTCT ATGCCATGAT TCACGCCTGC TGGTTGATCG CTGTCTTATA TCGACGCAGA 
AGAAAAGCCA TTGCGGAGAT CTGGCCCAAG TACTGCTGCT TCCTGGCCTG CATCATCACG 
TTCCAGTACT TCATCTGCAT CGGCATTCCT CCTGCTCCTT GCAGAGATTA CCCGTGGAGA 
TTCAAGGGTG CCAGCTTCAA CGACAACATC ATTAAGTGGC TGTACTTCCC AGACTTCATT 
GTGCGGCCCA ACCCCGTGTT TCTCGTCTAT GACTTCATGC TGCTTCTGTG TGCCTCCTTA 
CAAAGGCAAA TTTTTGAGGA TGAAAATAAG GCTGCAGTGC GAATCATGGC GGGAGACAAT 
GTCGAGATTT GCATGAACCT TGATGCAGCC TCTTTCAGCC AGCATAACCC TGTACCAGAC 
TTCATTCACT GCAGGTCGTA CTTAGACATG TCCAAAGTAA TCATCTTCAG CTACCTCTTC 
TGGTTCGTCC TCACGATCAT CTTCATCACT GGGACCACCA GGATCAGCAT CTTTTGCATG 
GGTTACTTGG TGGCCTGCTT CTATTTCCTG CTTTTTGGGG GCAATTTGCT GTTGAAACCC 
ATCAAGAGCA TCCTGCGCTA TTGGGACTGG CTGATTGCAT ACAACGTTTT TGTGATTACA 
ATGAAAAACA TACTGTCAAT AGGAGCATGT GGATACATTG AAAAATTGGT TCAAAATAGT 
TGCTGGTTGA TCCAAGCTTT CAGCCTGGCC TGCACCGTCA AAGGCTATAA AATGCCTGAT 
GACGATTCCT CATGTAAACT GCCCAGTGGT GAAGCAGGAA TTATCTGGGA CAGCATCTGT 
TTTGCCTTCC TCCTGCTGCA GAGGAGAGTT TTCATGAGTT ACTATTTTCT ACATGTCGTG 
GCAGATATAA AAGCTTCACA GATCCTGGCA TCAAGAGGGG CAGAACTTTT CCAGGCCACA 
ATTGTGAAGG CTGTAAAGGC CAGAATTGAG GAGGAGAAGA AGTCAATGGA TCAGTTGAAG 
AGGCAGATGG ATCGCATCAA GGCCAGACAA CAGAAATACA AAAAGGGTAA GGAGAGGATG 
CTGAGCTTGA CTCAGGAGTC AGGGGAAGGC CAAGACATCC AAAAGCTCTC TGAAGAGGAT 
GATGAAAGAG AAGCAGACAA ACAGAAAGCC AAAGGCAAAA AAAAGCAGTG GTGGCGGCCT 
TGGGTCGATC ATGCTTCCAT GGTCAGGAGT GGAGATTATT ATTTGTTTGA AACGGATAGT 
GAAGAAGAGG AAGAGGAAGA ATTTAAAAAG GAAGAAGAAG AGCCTCCAAG AAGGTCGGCC 
TTCCAGTTTG TTTATCAAGC CTGGATTACT GACCCTAAAA CAGCACTCCG TCAGAGGCGC 
AAAGAGAAAA AGAGATCTTC AAGAGAAGGC CAGAAACGAA GGAGAAAGGG ATCTGGGGAG 
GGTCCTGTGG AGTGGGAAGA TCGAGAGGAT GAACCAGTCA AAAAGAAATC TGATGGCCCA 
GATAATATCA TCAAGAGGAT ATTTAATATT TTGAAATTTA CCTGGGTCCT GTTTCTGGCA 
ACGGTGGATA GTTTCACAAC TTGGCTTAAT TCCATTTCAA GGGAGCATAT TGATATATCC 
ACAGTTCTGA GAATTGAAAG ATGCATGCTG ACCAGAGAAA TTAAAAAGGG CAACGTTCCC 
ACTCGGGAGA GCATCCACAT GTACTATCAG AACCACATCA TGAACCTTTC CCGAGAGTCT 
GGACTGGACA CCATCGACGA CCGTCCTGGT GCTGCTTCAG GTGCACAGAC AGCCCACAGG 
ATGGATAGCT TAGATTCTCA TGACAGTATC TCCAGCTGCT ACACCGAAGC AACCATGCTG 
TTCTCAAGGC AGTCTACCCT TGATGATTTA GATGGACCAG ACACTGTTCC TAAAACAAGT 
GAGCGTGCTC GACCTAGGCT CCGTAAAATG TTCAGCATGG ATGTGTCCTC CTCATCAGCT 
GACAGTGGCA GTTTAGCGTC CAGCGAGCCC ACCCAGTGCA CCATTCTGTC CTCGCGCCAG 
GGGACCACGG AAACCATCGA GGAGGTGGAG ACTGAGCAGG ACGAGGAGGC CGAGGGGGAC 
GAGGAGGCCG GGGAGGCTGA GGAGGCGGGG GTCGGCGACC CTGACGAGGC GGGTTTGGAG 
GCCCCAGAGG ATGGCGAGGA TGGCGTCGAG GAGGCCCCGC CGTCGTACAG CAAGGCCGTC 
AGCCTGGAGC GCCTGTCCTT GGACTCGCCG GACGGCTCCG CAGGCCGGAA CCACATGGCG 
GTCAGCCCCG ACGACAGCCG CTCCGACAGG CTGGAGTCCA GCATCTTACC CCCGCTGACT 
CACGAGCTGA CGGCCAGCGA GCTGCTGCTG AACAAGATGT TCCATGACGA TGAGCTGGAG 
GAGTCGGAGA AATTCTACGT TGGCCAGCCG CGGTTCTTGC TGCTCTTCTA CGCCATGTAC 
AACACCCTGG TGGCCCGCTC GGAAATGGTG TGCTATTTCG TCATCATCCT CAACCACATG 
GTCTCTGCCT CCATGATCAC CCTCGTGCTT CCCATCCTGA TCTTCCTCTG GGCCATGCTG 
TCTGTCCCCA GGCCCAGCAA GCGGTTCTGG ATGATGGCCA TTGTCTACAC AGAGGTGGCA 
ATTGTGGTCA AGTATTTCTT CCAGTTTGGG TTCTTTCCCT GGAACAAGCA CGTGGAATTG 
AACAAAGACA AACCTTACCA CCCGCCTAAT ATCATAGGAG TGGAAAAGAA AGAAGGTTAT 
GTCCTTTACG ACCTCATTCA GCTCCTGGCT CTGTTCTTCC ATCGATCGAT TCTGAAGTGC 
CACGGTTTAT GGGATGAAGA TGACGTCACC GACAGTGGCT TAGACAAGGA AGAGTCAGAT 
GACGAGCTCT CCCTCAGTCA CCGCAGAAGA GCTTCCTCCG ATTCTCTGAA ATCCATCAAC 
CTGGCCGCAT CGGCGGAGTC CGTGCACGTG TCCTTCCCCG AGCAGCCGGC AGCCGTCCGC 
AGGAAGCGCT CCGCCAGCAG CTCCCAGATA TCCCAGAGAT CCAGCTTTTC TTCAAACAGG 
TCCAAAAGAG GCAGCACAAG CACCCGAAAC AGCAGTCAAA AAGGAAGCAG TGTTTTGAGC 
ATTAAGCAAA AAAGCAAAAG GGAGCTTTAT ATGGAAAAGC TTCAAGAACA TTTAATCAAA 
GCAAAAGCAT TTACTATAAA GAAGACTCTG CAAATATATG TGCCCATCAG ACAGTTCTTC 
TACAACCTCA TCCACCCCGA CTACAGCGCC GTGACCGATG TGTATGTGCT CATGTTCCTG 
GCCGACACTG TCGACTTTGT CATCATTGTC TTTGGCTTCT GGGCCTTTGG GAAACACTCA 
GCAGCTGCAG ACATCACCTC TTCGCTGTCC GAGGACCAGG TCCCTGGGCC GTTTTTGGTG 
ATGGTCCTCA TCCAGTTTGG AACCATGGTG GTGGACCGAG CACTGTACCT CAGGAAGACT 
GTATTGGGAA AGGTCATTTT CCAGGTCATT CTTGTGTTTG GGATTCACTT CTGGATGTTC 
TTCATCTTGC CTGGCGTGAC GGAGAGGAAA TTCAGCCAGA ACCTGGTTGC TCAGCTTTGG 
TACTTTGTGA AGTGTGTTTA CTTCGGGCTG TCTGCCTACC AAATCCGTTG TGGCTACCCA 
ACTCGTGTCC TTGGAAACTT CCTCACAAAG AGCTACAATT ATGTCAACCT CTTCTTGTTT 
CAAGGGTTCC GCCTTGTTCC GTTTTTGACT GAGCTGAGGG CCGTGATGGA CTGGGTGTGG 
ACAGACACGA CCTTGAGCCT CTCCAGCTGG ATCTGTGTAG AGGACATCTA TGCTCACATA 
TTCATCCTGA AGTGTTGGCG GGAGTCAGAA AAAAGATACC CTCAGCCTCG GGGGCAGAAA 
AAGAAGAAGG TGGTAAAGTA TGGCATGGGG GGCATGATCA TCGTGCTGCT CATCTGCATC 
GTCTGGTTTC CTCTTCTCTT CATGTCCCTG ATCAAGTCTG TCGCTGGAGT GATCAACCAG 
CCCCTGGATG TCTCCGTCAC AATCACCCTG GGAGGGTACC AGCCTATTTT CACGATGAGT 
GCCCAGCAAA GCCAGTTGAA AGTTATGGAC CAGCCAAAGT TTAATAAATT TATAAAAGCT 
TTTTCTAGGG ACACTGGTGC TATGCAATTT CTGGAAAATT ATGAAAAAGA AGACATAACA 
GTAGCAGAAC TGGAAGGAAA CTCAAATTCT TTGTGGACCA TCAGCCCTCC CAGTAAGCAG 
AAAATGATAC ACGAACTCAC GGACCCCAAT AGTAGCTTCT CTGTTGTTTT TTCCTGGAGT 
ATTCAGAGAA ACATGAGTCT GGGTGCAAAA GCAGAAATAG CAACAGATAA GCTTTCCTTT 
CCTCTTAAAA ATACTACCCG AGAGAATATA GCTAAAATGA TAGCTGGCAA CAACACAGAA 
AGTTCCAAAA CACCTGTGAC TATAGAAAAG ATCTACCCGT ATTACGTGAA AGCACCCAGT 
GATTCCAACT CAAAACCTAT AAAGCAACTG TTATCTGAAA ATAATTTCAT GAACATCACC 
ATCATTTTGT CAAGAGATAC TTCAACTAAA TCTAACAGTG AGTGGTGGGT TCTCAACCTG 
ACTGGAAACA GAATATACAA CCAGCATGCT CAGGCCTTGG AGCTGGTGGT CTTCAATGAC 
AAAGTCAGCC CCCCTAGCCT GGGGTTCCTG GCTGGCTACG GTATCATGGG ACTATATGCT 
TCGGTTGTCC TCGTGATTGG GAAGTTTGTC CGTGAATTCT TCAGCGGGAT TTCTCACTCC 
ATCATGTTTG AGGAGCTTCC AAATGTCGAT CGAATTTTGA AGTTATGCAC GGATATTTTT 
TTGGTTCGAG AGACAGGGGA ACTGGAACTA GAGGAAGATC TCTATGCCAA ATTAATATTC 
CTGTATCGTT CACCAGAAAC CATGATCAAA TGGACCAGGG AAAAAACAAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIEZO2 ( XM_023647471.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023503239.1 Equus caballus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X1, mRNA.

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CloneID	OHb70014
Clone ID Related Accession (Same CDS sequence)	XM_023647471.1
Accession Version	XM_023647471.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	piezo-type mechanosensitive ion channel component 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009151.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGG AAGTGGTGTG CGGACTCATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
GTAGCGTGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTCATCTA CCTCTTGCTC 
ATCCCTCTGT TCTCAGAACC AACAAAAGCG ACGATGCAAG GACATACGGG ACGGTTGTTG 
AAGTCTCTGT GCTTCACCAG TCTGTCCTTC CTGTTGCTGC ACGTCATCTT TCACATCACA 
TTGGCCAGCC TGGAAGCTCA GCACCGTATC GCGCCTGGTT ACAACTGCTC GACGTGGGAA 
AAGACGCTTC GGCAGATTGG CTTTGAAAGC TTAAAGGGAG CCGACGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCGTCTGT 
AGGAACATTG TTCAGAAACC GGTGACAGAA GAAGCCGCAC AGTGTAACCC GGAGTTTCAA 
AATGAAGAAG TGGCTGGAGG AGAAAAAATA GATCCAGAAG AGGCACTGAT CTACGAAGAG 
GATTTAGATG AAGGAGATGG TGTTGAAGGA GAGTTGGAAG ACAGCACAAA ATTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCTTCCAAG CTTAAGGAGT TTATTGGCAA CATGATAACC 
ACCGCTGGAA AAGTTGTTGT GACAATTTTA CTGGGGTCAT CAGGTATGAT GCTGCCGTCT 
CTGACCTCGT CTGTGTACTT CTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACAGCA 
GGGCACTTAA TTGGACTTTA TTTATATCAG TTCCAATTCT TTCAAGAAGC GGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAATCAGTCA TTCAAACAGA CTGTTCCAGC 
ACGTGGAAGA TCATAGCGAA CCCAGACCTG TCCTGGTATC ACCACGCCAA CCCCATCCTC 
CTGCTGGTGA TGTACTACAC GCTGGCCACT CTCATCCGCA TCTGGCTACA GGAGCCGCTT 
GTGCAGGATG AGAGGACCAA GGAAGAGGAC AGAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACGGCCGAGA GGAGGCGGAG CCTGTGGTAC GCGACCCATT ACCCAACTGA CGAGAGAAAA 
CTTCTATCCA TGACTCAAGA TGACTACAAA CCATCTGATG GTCTGCTGGT GACTGTGAAC 
GGCAATCCTG TGGATTACCA CACCATCCAC CCCAGTCTTC CCATGGAGAA CGGCCCCGCC 
AAAGCCGACC TGTACTCCAC CCCGCAGTAC CGGTGGGAGC CCTCCGACGA CTCCTCGGAA 
AAGAAAGAGG AAGAAGAAGA TGAGAAAGAA GAATTTGAAG AGGAAAGGAG CCATGAGGAG 
AAAAGAAGTG TTAAAGTTCA CGCCATGGTC TCTGTGTTCC AGTTTATTAT GAAACAAAGT 
TACATCTGTG CCCTTATTGC TATGATGGCT TGGAGCATCA CCTATCACAG CTGGTTGACC 
TTCGTGCTGT TGATTTGGTC GTGCACTCTT TGGATGATTC GCAACAGGAG AAAATATGCC 
ATGATCAGCT CTCCTTTCAT GGTTGTTTAT GGAAATCTAC TGCTGATATT ACAGTATATA 
TGGAGTTTTG AACTTCCTGA AATTAAAAAG GTCCCAGGAT TTTTAGAAAA GAAAGAGCCA 
GGAGAACTTG CCTCAAAGAT ACTTTTCACC ATTACTTTTT GGCTACTGCT GAGGCAGCAC 
CTCACAGAGC AGAAAGCTCT TCGGGAAAAG GAAGCTCTTT TATCTGAGGT CAAAATTGGG 
AGTCAGGAAA ATGAAGAAAA AGCTGATGAG CAAGATATAC AGGTGGAAGG AGAGGCGGAA 
GAGAAGGAGG AAGGAGAGGA AGCAAAGCGA GAGAAGCAGG AGATAAAGAA GGAAGAGGAA 
GAGGAAGAGG AAGTGGAGGA AGGTGACGAT CAGGACATCA TGACGGTGCT GGGCAATCTG 
GTGGTGGCCA TGTTCATTAA GTACTGGATC TACGTCTGTG GAGGGATGTT CTTCTTCGTC 
AGCTTCGAGG GTAAAATTGT GATGTACAAA ATCATCTACA TGGTGCTGTT TCTATTCTGT 
GTGGCCTTGT ATCAGGTGCA CTATGAATGG TGGAGGCGAA TTCTAAAATA TTTTTGGATG 
TCAGTGGTTA TTTATACTAT GCTGGTGCTC ATCTTTATAT ACACATATCA GTTTGAAAAC 
TTCCCAGGCC TATGGCAAAA TATGACTGGA TTAAAAAGAG AAAAGCTTGA GGATCTTGGC 
TTAAAGCAGT TTACCGTGGC TGAACTATTC ACTCGCATAT TCATCCCAAC CTCCTTTCTC 
CTGGTGTGCA TTTTGCACCT TCACTATTTC CATGACCGGT TCCTGGAGCT CACAGACCTG 
AAGTCCATTC CCTGCAAGGA AGACAGCGCC ATCTACAGAC TGGCGCACCC TGAGGGAAGC 
CTGCCGGACC TCACCATGAT GAACCTGACC GCCAGCCTGC AGGCGCCGGA GATGGGGAAG 
CCGGCAGAGC CTGGTGCGGA GAAGCTGGAG GGGTGCCCTG AGAAAGCCGA GAAGGCTGAG 
CTTGGGAAAG ACAGTGAGGA GGAGGAGGAG GAGGAAGAGG AAGAGGAGGA GGCAGGGACG 
TCAGATTTAA GGAACAAATG GCACCTGGTG ATTGACCGCC TTACTGTGCT CTTCTTAAAA 
TTCCTGGAGT ATTTTCACAA GCTGCAGGTG TTCATGTGGT GGATTTTGGA GTTGCATATC 
ATCAAAATCG TTTCATCATA CATTATCTGG GTTTCTGTGA AAGAGGTGTC TCTGTTCAAC 
TATGTATTTT TGATTTCTTG GGCTTTTGCT CTGCCATATG CCAAGCTACG CCGTCTGGCT 
TCAAGTGTCT GCACTGTCTG GACGTGTGTG ATCATCGTCT GCAAAATGTT GTACCAGCTG 
CAAACCATTA AGCCTGAGAA CTTTTCTGTT AACTGTTCCT TGCCAAATGA AAACCAAACG 
AATGTCCCCC TCCAGCAGTT GAACAAGTCA CTGCTCTACA ACGCTCCTAT TGACCCGACA 
GAGTGGGTCG GCCTGAGGAA GTCCTCCCCT TTGCTGGTCT ACCTGAGGAA TAACCTCCTG 
ATGCTGGCCA TCCTGGCCTT CGAGGTTACA ATTTACCGCC ATCAGGAATA CTATCGAGGT 
CGGAACAATC TCACGGCCCC CGTGTCTAAA ACCATCTTTC ACGACATTAC GAGGCTCCAT 
CTAGATGATG GACTTATTAA TTGTGCCAAG TATTTCATAA ATTACTTCTT CTACAAGTTT 
GGCCTAGAGA CCTGTTTCCT AATGTCAGTG AACGTCATTG GGCAGCGAAT GGACTTCTAT 
GCCATGATTC ACGCCTGCTG GTTGATCGCT GTCTTATATC GACGCAGAAG AAAAGCCATT 
GCGGAGATCT GGCCCAAGTA CTGCTGCTTC CTGGCCTGCA TCATCACGTT CCAGTACTTC 
ATCTGCATCG GCATTCCTCC TGCTCCTTGC AGAGATTACC CGTGGAGATT CAAGGGTGCC 
AGCTTCAACG ACAACATCAT TAAGTGGCTG TACTTCCCAG ACTTCATTGT GCGGCCCAAC 
CCCGTGTTTC TCGTCTATGA CTTCATGCTG CTTCTGTGTG CCTCCTTACA AAGGCAAATT 
TTTGAGGATG AAAATAAGGC TGCAGTGCGA ATCATGGCGG GAGACAATGT CGAGATTTGC 
ATGAACCTTG ATGCAGCCTC TTTCAGCCAG CATAACCCTG TACCAGACTT CATTCACTGC 
AGGTCGTACT TAGACATGTC CAAAGTAATC ATCTTCAGCT ACCTCTTCTG GTTCGTCCTC 
ACGATCATCT TCATCACTGG GACCACCAGG ATCAGCATCT TTTGCATGGG TTACTTGGTG 
GCCTGCTTCT ATTTCCTGCT TTTTGGGGGC AATTTGCTGT TGAAACCCAT CAAGAGCATC 
CTGCGCTATT GGGACTGGCT GATTGCATAC AACGTTTTTG TGATTACAAT GAAAAACATA 
CTGTCAATAG GAGCATGTGG ATACATTGAA AAATTGGTTC AAAATAGTTG CTGGTTGATC 
CAAGCTTTCA GCCTGGCCTG CACCGTCAAA GGCTATAAAA TGCCTGATAA CGATTCCTCA 
TGTAAACTGC CCAGTGGTGA AGCAGGAATT ATCTGGGACA GCATCTGTTT TGCCTTCCTC 
CTGCTGCAGA GGAGAGTTTT CATGAGTTAC TATTTTCTAC ATGTCGTGGC AGATATAAAA 
GCTTCACAGA TCCTGGCATC AAGAGGGGCA GAACTTTTCC AGGCCACAAT TGTGAAGGCT 
GTAAAGGCCA GAATTGAGGA GGAGAAGAAG TCAATGGATC AGTTGAAGAG GCAGATGGAT 
CGCATCAAGG CCAGACAACA GAAATACAAA AAGGGTAAGG AGAGGATGCT GAGCTTGACT 
CAGGAGTCAG GGGAAGGCCA AGACATCCAA AAGCTCTCTG AAGAGGATGA TGAAAGAGAA 
GCAGACAAAC AGAAAGCCAA AGGCAAAAAA AAGCAGTGGT GGCGGCCTTG GGTCGATCAT 
GCTTCCATGG TCAGGAGTGG AGATTATTAT TTGTTTGAAA CGGATAGTGA AGAAGAGGAA 
GAGGAAGAAT TTAAAAAGGA AGAAGAAGAG CCTCCAAGAA GGTCGGCCTT CCAGTTTGTT 
TATCAAGCCT GGATTACTGA CCCTAAAACA GCACTCCGTC AGAGGCGCAA AGAGAAAAAG 
AGATCTTCAA GAGAAGGCCA GAAACGAAGG AGAAAGGGAT CTGGGGAGGG TCCTGTGGAG 
TGGGAAGATC GAGAGGATGA ACCAGTCAAA AAGAAATCTG ATGGCCCAGA TAATATCATC 
AAGAGGATAT TTAATATTTT GAAATTTACC TGGGTCCTGT TTCTGGCAAC GGTGGATAGT 
TTCACAACTT GGCTTAATTC CATTTCAAGG GAGCATATTG ATATATCCAC AGTTCTGAGA 
ATTGAAAGAT GCATGCTGAC CAGAGAAATT AAAAAGGGCA ACGTTCCCAC TCGGGAGAGC 
ATCCACATGT ACTATCAGAA CCACATCATG AACCTTTCCC GAGAGTCTGG ACTGGACACC 
ATCGACGACC GTCCTGGTGC TGCTTCAGGT GCACAGACAG CCCACAGGAT GGATAGCTTA 
GATTCTCATG ACAGTATCTC CAGCGAGCCC ACCCAGTGCA CCATTCTGTC CTCGCGCCAG 
GGGACCACGG AAACCATCGA GGAGGTGGAG ACTGAGCAGG ACGAGGAGGC CGAGGGGGAC 
GAGGAGGCCG GGGAGGCTGA GGAGGCGGGG GTCGGCGACC CTGACGAGGC GGGTTTGGAG 
GCCCCAGAGG ATGGCGAGGA TGGCGTCGAG GAGGCCCCGC CGTCGTACAG CAAGGCCGTC 
AGCCTGGAGC GCCTGTCCTT GGACTCGCCG GACGGCTCCG CAGGCCGGAA CCACATGGCG 
GTCAGCCCCG ACGACAGCCG CTCCGACAGG CTGGAGTCCA GCATCTTACC CCCGCTGACT 
CACGAGCTGA CGGCCAGCGA GCTGCTGCTG AACAAGATGT TCCATGACGA TGAGCTGGAG 
GAGTCGGAGA AATTCTACGT TGGCCAGCCG CGGTTCTTGC TGCTCTTCTA CGCCATGTAC 
AACACCCTGG TGGCCCGCTC GGAAATGGTG TGCTATTTCG TCATCATCCT CAACCACATG 
GTCTCTGCCT CCATGATCAC CCTCGTGCTT CCCATCCTGA TCTTCCTCTG GGCCATGCTG 
TCTGTCCCCA GGCCCAGCAA GCGGTTCTGG ATGATGGCCA TTGTCTACAC AGAGGTGGCA 
ATTGTGGTCA AGTATTTCTT CCAGTTTGGG TTCTTTCCCT GGAACAAGCA CGTGGAATTG 
AACAAAGACA AACCTTACCA CCCGCCTAAT ATCATAGGAG TGGAAAAGAA AGAAGGTTAT 
GTCCTTTACG ACCTCATTCA GCTCCTGGCT CTGTTCTTCC ATCGATCGAT TCTGAAGTGC 
CACGGTTTAT GGGATGAAGA TGACGTCACC GACAGTGGCT TAGACAAGGA AGAGTCAGAT 
GACGAGCTCT CCCTCAGTCA CCGCAGAAGA GCTTCCTCCG ATTCTCTGAA ATCCATCAAC 
CTGGCCGCAT CGGCGGAGTC CGTGCACGTG TCCTTCCCCG AGCAGCCGGC AGCCGTCCGC 
AGGAAGCGCT CCGCCAGCAG CTCCCAGATA TCCCAGAGAT CCAGCTTTTC TTCAAACAGG 
TCCAAAAGAG GCAGCACAAG CACCCGAAAC AGCAGTCAAA AAGGAAGCAG TGTTTTGAGC 
ATTAAGCAAA AAAGCAAAAG GGAGCTTTAT ATGGAAAAGC TTCAAGAACA TTTAATCAAA 
GCAAAAGCAT TTACTATAAA GAAGACTCTG CAAATATATG TGCCCATCAG ACAGTTCTTC 
TACAACCTCA TCCACCCCGA CTACAGCGCC GTGACCGATG TGTATGTGCT CATGTTCCTG 
GCCGACACTG TCGACTTTGT CATCATTGTC TTTGGCTTCT GGGCCTTTGG GAAACACTCA 
GCAGCTGCAG ACATCACCTC TTCGCTGTCC GAGGACCAGG TCCCTGGGCC GTTTTTGGTG 
ATGGTCCTCA TCCAGTTTGG AACCATGGTG GTGGACCGAG CACTGTACCT CAGGAAGACT 
GTATTGGGAA AGGTCATTTT CCAGGTCATT CTTGTGTTTG GGATTCACTT CTGGATGTTC 
TTCATCTTGC CTGGCGTGAC GGAGAGGAAA TTCAGCCAGA ACCTGGTTGC TCAGCTTTGG 
TACTTTGTGA AGTGTGTTTA CTTCGGGCTG TCTGCCTACC AAATCCGTTG TGGCTACCCA 
ACTCGTGTCC TTGGAAACTT CCTCACAAAG AGCTACAATT ATGTCAACCT CTTCTTGTTT 
CAAGGGTTCC GCCTTGTTCC GTTTTTGACT GAGCTGAGGG CCGTGATGGA CTGGGTGTGG 
ACAGACACGA CCTTGAGCCT CTCCAGCTGG ATCTGTGTAG AGGACATCTA TGCTCACATA 
TTCATCCTGA AGTGTTGGCG GGAGTCAGAA AAAAGATACC CTCAGCCTCG GGGGCAGAAA 
AAGAAGAAGG TGGTAAAGTA TGGCATGGGG GGCATGATCA TCGTGCTGCT CATCTGCATC 
GTCTGGTTTC CTCTTCTCTT CATGTCCCTG ATCAAGTCTG TCGCTGGAGT GATCAACCAG 
CCCCTGGATG TCTCCGTCAC AATCACCCTG GGAGGGTACC AGCCTATTTT CACGATGAGT 
GCCCAGCAAA GCCAGTTGAA AGTTATGGAC CAGCCAAAGT TTAATAAATT TATAAAAGCT 
TTTTCTAGGG ACACTGGTGC TATGCAATTT CTGGAAAATT ATGAAAAAGA AGACATAACA 
GTAGCAGAAC TGGAAGGAAA CTCAAATTCT TTGTGGACCA TCAGCCCTCC CAGTAAGCAG 
AAAATGATAC ACGAACTCAC GGACCCCAAT AGTAGCTTCT CTGTTGTTTT TTCCTGGAGT 
ATTCAGAGAA ACATGAGTCT GGGTGCAAAA GCAGAAATAG CAACAGATAA GCTTTCCTTT 
CCTCTTAAAA ATACTACCCG AGAGAATATA GCTAAAATGA TAGCTGGCAA CAACACAGAA 
AGTTCCAAAA CACCTGTGAC TATAGAAAAG ATCTACCCGT ATTACGTGAA AGCACCCAGT 
GATTCCAACT CAAAACCTAT AAAGCAACTG TTATCTGAAA ATAATTTCAT GAACATCACC 
ATCATTTTGT CAAGAGATAC TTCAACTAAA TCTAACAGTG AGTGGTGGGT TCTCAACCTG 
ACTGGAAACA GAATATACAA CCAGCATGCT CAGGCCTTGG AGCTGGTGGT CTTCAATGAC 
AAAGTCAGCC CCCCTAGCCT GGGGTTCCTG GCTGGCTACG GTATCATGGG ACTATATGCT 
TCGGTTGTCC TCGTGATTGG GAAGTTTGTC CGTGAATTCT TCAGCGGGAT TTCTCACTCC 
ATCATGTTTG AGGAGCTTCC AAATGTCGAT CGAATTTTGA AGTTATGCAC GGATATTTTT 
TTGGTTCGAG AGACAGGGGA ACTGGAACTA GAGGAAGATC TCTATGCCAA ATTAATATTC 
CTGTATCGTT CACCAGAAAC CATGATCAAA TGGACCAGGG AAAAAACAAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023503239.1
CDS	40..8253
Translation	MASEVVCGLIFRLLLPICLAVACAFRYNGLSFVYLIYLLLIPLFSEPTKATMQGHTGRLLKSLCFTSLSFLLLHVIFHITLASLEAQHRIAPGYNCSTWEKTLRQIGFESLKGADAGNGIRVFVPDIGMFIASLTIWLVCRNIVQKPVTEEAAQCNPEFQNEEVAGGEKIDPEEALIYEEDLDEGDGVEGELEDSTKLKMFRRLASVASKLKEFIGNMITTAGKVVVTILLGSSGMMLPSLTSSVYFFVFLGLCTWWSWCRTFDPLLFSCLCVLLAIFTAGHLIGLYLYQFQFFQEAVPPNDYYARLFGIKSVIQTDCSSTWKIIANPDLSWYHHANPILLLVMYYTLATLIRIWLQEPLVQDERTKEEDRALACSPIQITAERRRSLWYATHYPTDERKLLSMTQDDYKPSDGLLVTVNGNPVDYHTIHPSLPMENGPAKADLYSTPQYRWEPSDDSSEKKEEEEDEKEEFEEERSHEEKRSVKVHAMVSVFQFIMKQSYICALIAMMAWSITYHSWLTFVLLIWSCTLWMIRNRRKYAMISSPFMVVYGNLLLILQYIWSFELPEIKKVPGFLEKKEPGELASKILFTITFWLLLRQHLTEQKALREKEALLSEVKIGSQENEEKADEQDIQVEGEAEEKEEGEEAKREKQEIKKEEEEEEEVEEGDDQDIMTVLGNLVVAMFIKYWIYVCGGMFFFVSFEGKIVMYKIIYMVLFLFCVALYQVHYEWWRRILKYFWMSVVIYTMLVLIFIYTYQFENFPGLWQNMTGLKREKLEDLGLKQFTVAELFTRIFIPTSFLLVCILHLHYFHDRFLELTDLKSIPCKEDSAIYRLAHPEGSLPDLTMMNLTASLQAPEMGKPAEPGAEKLEGCPEKAEKAELGKDSEEEEEEEEEEEEAGTSDLRNKWHLVIDRLTVLFLKFLEYFHKLQVFMWWILELHIIKIVSSYIIWVSVKEVSLFNYVFLISWAFALPYAKLRRLASSVCTVWTCVIIVCKMLYQLQTIKPENFSVNCSLPNENQTNVPLQQLNKSLLYNAPIDPTEWVGLRKSSPLLVYLRNNLLMLAILAFEVTIYRHQEYYRGRNNLTAPVSKTIFHDITRLHLDDGLINCAKYFINYFFYKFGLETCFLMSVNVIGQRMDFYAMIHACWLIAVLYRRRRKAIAEIWPKYCCFLACIITFQYFICIGIPPAPCRDYPWRFKGASFNDNIIKWLYFPDFIVRPNPVFLVYDFMLLLCASLQRQIFEDENKAAVRIMAGDNVEICMNLDAASFSQHNPVPDFIHCRSYLDMSKVIIFSYLFWFVLTIIFITGTTRISIFCMGYLVACFYFLLFGGNLLLKPIKSILRYWDWLIAYNVFVITMKNILSIGACGYIEKLVQNSCWLIQAFSLACTVKGYKMPDNDSSCKLPSGEAGIIWDSICFAFLLLQRRVFMSYYFLHVVADIKASQILASRGAELFQATIVKAVKARIEEEKKSMDQLKRQMDRIKARQQKYKKGKERMLSLTQESGEGQDIQKLSEEDDEREADKQKAKGKKKQWWRPWVDHASMVRSGDYYLFETDSEEEEEEEFKKEEEEPPRRSAFQFVYQAWITDPKTALRQRRKEKKRSSREGQKRRRKGSGEGPVEWEDREDEPVKKKSDGPDNIIKRIFNILKFTWVLFLATVDSFTTWLNSISREHIDISTVLRIERCMLTREIKKGNVPTRESIHMYYQNHIMNLSRESGLDTIDDRPGAASGAQTAHRMDSLDSHDSISSEPTQCTILSSRQGTTETIEEVETEQDEEAEGDEEAGEAEEAGVGDPDEAGLEAPEDGEDGVEEAPPSYSKAVSLERLSLDSPDGSAGRNHMAVSPDDSRSDRLESSILPPLTHELTASELLLNKMFHDDELEESEKFYVGQPRFLLLFYAMYNTLVARSEMVCYFVIILNHMVSASMITLVLPILIFLWAMLSVPRPSKRFWMMAIVYTEVAIVVKYFFQFGFFPWNKHVELNKDKPYHPPNIIGVEKKEGYVLYDLIQLLALFFHRSILKCHGLWDEDDVTDSGLDKEESDDELSLSHRRRASSDSLKSINLAASAESVHVSFPEQPAAVRRKRSASSSQISQRSSFSSNRSKRGSTSTRNSSQKGSSVLSIKQKSKRELYMEKLQEHLIKAKAFTIKKTLQIYVPIRQFFYNLIHPDYSAVTDVYVLMFLADTVDFVIIVFGFWAFGKHSAAADITSSLSEDQVPGPFLVMVLIQFGTMVVDRALYLRKTVLGKVIFQVILVFGIHFWMFFILPGVTERKFSQNLVAQLWYFVKCVYFGLSAYQIRCGYPTRVLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFILKCWRESEKRYPQPRGQKKKKVVKYGMGGMIIVLLICIVWFPLLFMSLIKSVAGVINQPLDVSVTITLGGYQPIFTMSAQQSQLKVMDQPKFNKFIKAFSRDTGAMQFLENYEKEDITVAELEGNSNSLWTISPPSKQKMIHELTDPNSSFSVVFSWSIQRNMSLGAKAEIATDKLSFPLKNTTRENIAKMIAGNNTESSKTPVTIEKIYPYYVKAPSDSNSKPIKQLLSENNFMNITIILSRDTSTKSNSEWWVLNLTGNRIYNQHAQALELVVFNDKVSPPSLGFLAGYGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCTDIFLVRETGELELEEDLYAKLIFLYRSPETMIKWTREKTN

Target ORF information:

RefSeq Version	XM_023647471.1
Organism	Equus caballus(horse)
Definition	Equus caballus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023647471.1

ORF Insert Sequence:

ATGGCCTCGG AAGTGGTGTG CGGACTCATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
GTAGCGTGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTCATCTA CCTCTTGCTC 
ATCCCTCTGT TCTCAGAACC AACAAAAGCG ACGATGCAAG GACATACGGG ACGGTTGTTG 
AAGTCTCTGT GCTTCACCAG TCTGTCCTTC CTGTTGCTGC ACGTCATCTT TCACATCACA 
TTGGCCAGCC TGGAAGCTCA GCACCGTATC GCGCCTGGTT ACAACTGCTC GACGTGGGAA 
AAGACGCTTC GGCAGATTGG CTTTGAAAGC TTAAAGGGAG CCGACGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCGTCTGT 
AGGAACATTG TTCAGAAACC GGTGACAGAA GAAGCCGCAC AGTGTAACCC GGAGTTTCAA 
AATGAAGAAG TGGCTGGAGG AGAAAAAATA GATCCAGAAG AGGCACTGAT CTACGAAGAG 
GATTTAGATG AAGGAGATGG TGTTGAAGGA GAGTTGGAAG ACAGCACAAA ATTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCTTCCAAG CTTAAGGAGT TTATTGGCAA CATGATAACC 
ACCGCTGGAA AAGTTGTTGT GACAATTTTA CTGGGGTCAT CAGGTATGAT GCTGCCGTCT 
CTGACCTCGT CTGTGTACTT CTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACAGCA 
GGGCACTTAA TTGGACTTTA TTTATATCAG TTCCAATTCT TTCAAGAAGC GGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAATCAGTCA TTCAAACAGA CTGTTCCAGC 
ACGTGGAAGA TCATAGCGAA CCCAGACCTG TCCTGGTATC ACCACGCCAA CCCCATCCTC 
CTGCTGGTGA TGTACTACAC GCTGGCCACT CTCATCCGCA TCTGGCTACA GGAGCCGCTT 
GTGCAGGATG AGAGGACCAA GGAAGAGGAC AGAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACGGCCGAGA GGAGGCGGAG CCTGTGGTAC GCGACCCATT ACCCAACTGA CGAGAGAAAA 
CTTCTATCCA TGACTCAAGA TGACTACAAA CCATCTGATG GTCTGCTGGT GACTGTGAAC 
GGCAATCCTG TGGATTACCA CACCATCCAC CCCAGTCTTC CCATGGAGAA CGGCCCCGCC 
AAAGCCGACC TGTACTCCAC CCCGCAGTAC CGGTGGGAGC CCTCCGACGA CTCCTCGGAA 
AAGAAAGAGG AAGAAGAAGA TGAGAAAGAA GAATTTGAAG AGGAAAGGAG CCATGAGGAG 
AAAAGAAGTG TTAAAGTTCA CGCCATGGTC TCTGTGTTCC AGTTTATTAT GAAACAAAGT 
TACATCTGTG CCCTTATTGC TATGATGGCT TGGAGCATCA CCTATCACAG CTGGTTGACC 
TTCGTGCTGT TGATTTGGTC GTGCACTCTT TGGATGATTC GCAACAGGAG AAAATATGCC 
ATGATCAGCT CTCCTTTCAT GGTTGTTTAT GGAAATCTAC TGCTGATATT ACAGTATATA 
TGGAGTTTTG AACTTCCTGA AATTAAAAAG GTCCCAGGAT TTTTAGAAAA GAAAGAGCCA 
GGAGAACTTG CCTCAAAGAT ACTTTTCACC ATTACTTTTT GGCTACTGCT GAGGCAGCAC 
CTCACAGAGC AGAAAGCTCT TCGGGAAAAG GAAGCTCTTT TATCTGAGGT CAAAATTGGG 
AGTCAGGAAA ATGAAGAAAA AGCTGATGAG CAAGATATAC AGGTGGAAGG AGAGGCGGAA 
GAGAAGGAGG AAGGAGAGGA AGCAAAGCGA GAGAAGCAGG AGATAAAGAA GGAAGAGGAA 
GAGGAAGAGG AAGTGGAGGA AGGTGACGAT CAGGACATCA TGACGGTGCT GGGCAATCTG 
GTGGTGGCCA TGTTCATTAA GTACTGGATC TACGTCTGTG GAGGGATGTT CTTCTTCGTC 
AGCTTCGAGG GTAAAATTGT GATGTACAAA ATCATCTACA TGGTGCTGTT TCTATTCTGT 
GTGGCCTTGT ATCAGGTGCA CTATGAATGG TGGAGGCGAA TTCTAAAATA TTTTTGGATG 
TCAGTGGTTA TTTATACTAT GCTGGTGCTC ATCTTTATAT ACACATATCA GTTTGAAAAC 
TTCCCAGGCC TATGGCAAAA TATGACTGGA TTAAAAAGAG AAAAGCTTGA GGATCTTGGC 
TTAAAGCAGT TTACCGTGGC TGAACTATTC ACTCGCATAT TCATCCCAAC CTCCTTTCTC 
CTGGTGTGCA TTTTGCACCT TCACTATTTC CATGACCGGT TCCTGGAGCT CACAGACCTG 
AAGTCCATTC CCTGCAAGGA AGACAGCGCC ATCTACAGAC TGGCGCACCC TGAGGGAAGC 
CTGCCGGACC TCACCATGAT GAACCTGACC GCCAGCCTGC AGGCGCCGGA GATGGGGAAG 
CCGGCAGAGC CTGGTGCGGA GAAGCTGGAG GGGTGCCCTG AGAAAGCCGA GAAGGCTGAG 
CTTGGGAAAG ACAGTGAGGA GGAGGAGGAG GAGGAAGAGG AAGAGGAGGA GGCAGGGACG 
TCAGATTTAA GGAACAAATG GCACCTGGTG ATTGACCGCC TTACTGTGCT CTTCTTAAAA 
TTCCTGGAGT ATTTTCACAA GCTGCAGGTG TTCATGTGGT GGATTTTGGA GTTGCATATC 
ATCAAAATCG TTTCATCATA CATTATCTGG GTTTCTGTGA AAGAGGTGTC TCTGTTCAAC 
TATGTATTTT TGATTTCTTG GGCTTTTGCT CTGCCATATG CCAAGCTACG CCGTCTGGCT 
TCAAGTGTCT GCACTGTCTG GACGTGTGTG ATCATCGTCT GCAAAATGTT GTACCAGCTG 
CAAACCATTA AGCCTGAGAA CTTTTCTGTT AACTGTTCCT TGCCAAATGA AAACCAAACG 
AATGTCCCCC TCCAGCAGTT GAACAAGTCA CTGCTCTACA ACGCTCCTAT TGACCCGACA 
GAGTGGGTCG GCCTGAGGAA GTCCTCCCCT TTGCTGGTCT ACCTGAGGAA TAACCTCCTG 
ATGCTGGCCA TCCTGGCCTT CGAGGTTACA ATTTACCGCC ATCAGGAATA CTATCGAGGT 
CGGAACAATC TCACGGCCCC CGTGTCTAAA ACCATCTTTC ACGACATTAC GAGGCTCCAT 
CTAGATGATG GACTTATTAA TTGTGCCAAG TATTTCATAA ATTACTTCTT CTACAAGTTT 
GGCCTAGAGA CCTGTTTCCT AATGTCAGTG AACGTCATTG GGCAGCGAAT GGACTTCTAT 
GCCATGATTC ACGCCTGCTG GTTGATCGCT GTCTTATATC GACGCAGAAG AAAAGCCATT 
GCGGAGATCT GGCCCAAGTA CTGCTGCTTC CTGGCCTGCA TCATCACGTT CCAGTACTTC 
ATCTGCATCG GCATTCCTCC TGCTCCTTGC AGAGATTACC CGTGGAGATT CAAGGGTGCC 
AGCTTCAACG ACAACATCAT TAAGTGGCTG TACTTCCCAG ACTTCATTGT GCGGCCCAAC 
CCCGTGTTTC TCGTCTATGA CTTCATGCTG CTTCTGTGTG CCTCCTTACA AAGGCAAATT 
TTTGAGGATG AAAATAAGGC TGCAGTGCGA ATCATGGCGG GAGACAATGT CGAGATTTGC 
ATGAACCTTG ATGCAGCCTC TTTCAGCCAG CATAACCCTG TACCAGACTT CATTCACTGC 
AGGTCGTACT TAGACATGTC CAAAGTAATC ATCTTCAGCT ACCTCTTCTG GTTCGTCCTC 
ACGATCATCT TCATCACTGG GACCACCAGG ATCAGCATCT TTTGCATGGG TTACTTGGTG 
GCCTGCTTCT ATTTCCTGCT TTTTGGGGGC AATTTGCTGT TGAAACCCAT CAAGAGCATC 
CTGCGCTATT GGGACTGGCT GATTGCATAC AACGTTTTTG TGATTACAAT GAAAAACATA 
CTGTCAATAG GAGCATGTGG ATACATTGAA AAATTGGTTC AAAATAGTTG CTGGTTGATC 
CAAGCTTTCA GCCTGGCCTG CACCGTCAAA GGCTATAAAA TGCCTGATAA CGATTCCTCA 
TGTAAACTGC CCAGTGGTGA AGCAGGAATT ATCTGGGACA GCATCTGTTT TGCCTTCCTC 
CTGCTGCAGA GGAGAGTTTT CATGAGTTAC TATTTTCTAC ATGTCGTGGC AGATATAAAA 
GCTTCACAGA TCCTGGCATC AAGAGGGGCA GAACTTTTCC AGGCCACAAT TGTGAAGGCT 
GTAAAGGCCA GAATTGAGGA GGAGAAGAAG TCAATGGATC AGTTGAAGAG GCAGATGGAT 
CGCATCAAGG CCAGACAACA GAAATACAAA AAGGGTAAGG AGAGGATGCT GAGCTTGACT 
CAGGAGTCAG GGGAAGGCCA AGACATCCAA AAGCTCTCTG AAGAGGATGA TGAAAGAGAA 
GCAGACAAAC AGAAAGCCAA AGGCAAAAAA AAGCAGTGGT GGCGGCCTTG GGTCGATCAT 
GCTTCCATGG TCAGGAGTGG AGATTATTAT TTGTTTGAAA CGGATAGTGA AGAAGAGGAA 
GAGGAAGAAT TTAAAAAGGA AGAAGAAGAG CCTCCAAGAA GGTCGGCCTT CCAGTTTGTT 
TATCAAGCCT GGATTACTGA CCCTAAAACA GCACTCCGTC AGAGGCGCAA AGAGAAAAAG 
AGATCTTCAA GAGAAGGCCA GAAACGAAGG AGAAAGGGAT CTGGGGAGGG TCCTGTGGAG 
TGGGAAGATC GAGAGGATGA ACCAGTCAAA AAGAAATCTG ATGGCCCAGA TAATATCATC 
AAGAGGATAT TTAATATTTT GAAATTTACC TGGGTCCTGT TTCTGGCAAC GGTGGATAGT 
TTCACAACTT GGCTTAATTC CATTTCAAGG GAGCATATTG ATATATCCAC AGTTCTGAGA 
ATTGAAAGAT GCATGCTGAC CAGAGAAATT AAAAAGGGCA ACGTTCCCAC TCGGGAGAGC 
ATCCACATGT ACTATCAGAA CCACATCATG AACCTTTCCC GAGAGTCTGG ACTGGACACC 
ATCGACGACC GTCCTGGTGC TGCTTCAGGT GCACAGACAG CCCACAGGAT GGATAGCTTA 
GATTCTCATG ACAGTATCTC CAGCGAGCCC ACCCAGTGCA CCATTCTGTC CTCGCGCCAG 
GGGACCACGG AAACCATCGA GGAGGTGGAG ACTGAGCAGG ACGAGGAGGC CGAGGGGGAC 
GAGGAGGCCG GGGAGGCTGA GGAGGCGGGG GTCGGCGACC CTGACGAGGC GGGTTTGGAG 
GCCCCAGAGG ATGGCGAGGA TGGCGTCGAG GAGGCCCCGC CGTCGTACAG CAAGGCCGTC 
AGCCTGGAGC GCCTGTCCTT GGACTCGCCG GACGGCTCCG CAGGCCGGAA CCACATGGCG 
GTCAGCCCCG ACGACAGCCG CTCCGACAGG CTGGAGTCCA GCATCTTACC CCCGCTGACT 
CACGAGCTGA CGGCCAGCGA GCTGCTGCTG AACAAGATGT TCCATGACGA TGAGCTGGAG 
GAGTCGGAGA AATTCTACGT TGGCCAGCCG CGGTTCTTGC TGCTCTTCTA CGCCATGTAC 
AACACCCTGG TGGCCCGCTC GGAAATGGTG TGCTATTTCG TCATCATCCT CAACCACATG 
GTCTCTGCCT CCATGATCAC CCTCGTGCTT CCCATCCTGA TCTTCCTCTG GGCCATGCTG 
TCTGTCCCCA GGCCCAGCAA GCGGTTCTGG ATGATGGCCA TTGTCTACAC AGAGGTGGCA 
ATTGTGGTCA AGTATTTCTT CCAGTTTGGG TTCTTTCCCT GGAACAAGCA CGTGGAATTG 
AACAAAGACA AACCTTACCA CCCGCCTAAT ATCATAGGAG TGGAAAAGAA AGAAGGTTAT 
GTCCTTTACG ACCTCATTCA GCTCCTGGCT CTGTTCTTCC ATCGATCGAT TCTGAAGTGC 
CACGGTTTAT GGGATGAAGA TGACGTCACC GACAGTGGCT TAGACAAGGA AGAGTCAGAT 
GACGAGCTCT CCCTCAGTCA CCGCAGAAGA GCTTCCTCCG ATTCTCTGAA ATCCATCAAC 
CTGGCCGCAT CGGCGGAGTC CGTGCACGTG TCCTTCCCCG AGCAGCCGGC AGCCGTCCGC 
AGGAAGCGCT CCGCCAGCAG CTCCCAGATA TCCCAGAGAT CCAGCTTTTC TTCAAACAGG 
TCCAAAAGAG GCAGCACAAG CACCCGAAAC AGCAGTCAAA AAGGAAGCAG TGTTTTGAGC 
ATTAAGCAAA AAAGCAAAAG GGAGCTTTAT ATGGAAAAGC TTCAAGAACA TTTAATCAAA 
GCAAAAGCAT TTACTATAAA GAAGACTCTG CAAATATATG TGCCCATCAG ACAGTTCTTC 
TACAACCTCA TCCACCCCGA CTACAGCGCC GTGACCGATG TGTATGTGCT CATGTTCCTG 
GCCGACACTG TCGACTTTGT CATCATTGTC TTTGGCTTCT GGGCCTTTGG GAAACACTCA 
GCAGCTGCAG ACATCACCTC TTCGCTGTCC GAGGACCAGG TCCCTGGGCC GTTTTTGGTG 
ATGGTCCTCA TCCAGTTTGG AACCATGGTG GTGGACCGAG CACTGTACCT CAGGAAGACT 
GTATTGGGAA AGGTCATTTT CCAGGTCATT CTTGTGTTTG GGATTCACTT CTGGATGTTC 
TTCATCTTGC CTGGCGTGAC GGAGAGGAAA TTCAGCCAGA ACCTGGTTGC TCAGCTTTGG 
TACTTTGTGA AGTGTGTTTA CTTCGGGCTG TCTGCCTACC AAATCCGTTG TGGCTACCCA 
ACTCGTGTCC TTGGAAACTT CCTCACAAAG AGCTACAATT ATGTCAACCT CTTCTTGTTT 
CAAGGGTTCC GCCTTGTTCC GTTTTTGACT GAGCTGAGGG CCGTGATGGA CTGGGTGTGG 
ACAGACACGA CCTTGAGCCT CTCCAGCTGG ATCTGTGTAG AGGACATCTA TGCTCACATA 
TTCATCCTGA AGTGTTGGCG GGAGTCAGAA AAAAGATACC CTCAGCCTCG GGGGCAGAAA 
AAGAAGAAGG TGGTAAAGTA TGGCATGGGG GGCATGATCA TCGTGCTGCT CATCTGCATC 
GTCTGGTTTC CTCTTCTCTT CATGTCCCTG ATCAAGTCTG TCGCTGGAGT GATCAACCAG 
CCCCTGGATG TCTCCGTCAC AATCACCCTG GGAGGGTACC AGCCTATTTT CACGATGAGT 
GCCCAGCAAA GCCAGTTGAA AGTTATGGAC CAGCCAAAGT TTAATAAATT TATAAAAGCT 
TTTTCTAGGG ACACTGGTGC TATGCAATTT CTGGAAAATT ATGAAAAAGA AGACATAACA 
GTAGCAGAAC TGGAAGGAAA CTCAAATTCT TTGTGGACCA TCAGCCCTCC CAGTAAGCAG 
AAAATGATAC ACGAACTCAC GGACCCCAAT AGTAGCTTCT CTGTTGTTTT TTCCTGGAGT 
ATTCAGAGAA ACATGAGTCT GGGTGCAAAA GCAGAAATAG CAACAGATAA GCTTTCCTTT 
CCTCTTAAAA ATACTACCCG AGAGAATATA GCTAAAATGA TAGCTGGCAA CAACACAGAA 
AGTTCCAAAA CACCTGTGAC TATAGAAAAG ATCTACCCGT ATTACGTGAA AGCACCCAGT 
GATTCCAACT CAAAACCTAT AAAGCAACTG TTATCTGAAA ATAATTTCAT GAACATCACC 
ATCATTTTGT CAAGAGATAC TTCAACTAAA TCTAACAGTG AGTGGTGGGT TCTCAACCTG 
ACTGGAAACA GAATATACAA CCAGCATGCT CAGGCCTTGG AGCTGGTGGT CTTCAATGAC 
AAAGTCAGCC CCCCTAGCCT GGGGTTCCTG GCTGGCTACG GTATCATGGG ACTATATGCT 
TCGGTTGTCC TCGTGATTGG GAAGTTTGTC CGTGAATTCT TCAGCGGGAT TTCTCACTCC 
ATCATGTTTG AGGAGCTTCC AAATGTCGAT CGAATTTTGA AGTTATGCAC GGATATTTTT 
TTGGTTCGAG AGACAGGGGA ACTGGAACTA GAGGAAGATC TCTATGCCAA ATTAATATTC 
CTGTATCGTT CACCAGAAAC CATGATCAAA TGGACCAGGG AAAAAACAAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIEZO2 ( XM_023647472.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023503240.1 Equus caballus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X2, mRNA.

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CloneID	OHb70015
Clone ID Related Accession (Same CDS sequence)	XM_023647472.1
Accession Version	XM_023647472.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1516550400000
Organism	Equus caballus(horse)
Product	piezo-type mechanosensitive ion channel component 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009151.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGG AAGTGGTGTG CGGACTCATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
GTAGCGTGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTCATCTA CCTCTTGCTC 
ATCCCTCTGT TCTCAGAACC AACAAAAGCG ACGATGCAAG GACATACGGG ACGGTTGTTG 
AAGTCTCTGT GCTTCACCAG TCTGTCCTTC CTGTTGCTGC ACGTCATCTT TCACATCACA 
TTGGCCAGCC TGGAAGCTCA GCACCGTATC GCGCCTGGTT ACAACTGCTC GACGTGGGAA 
AAGACGCTTC GGCAGATTGG CTTTGAAAGC TTAAAGGGAG CCGACGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCGTCTGT 
AGGAACATTG TTCAGAAACC GGTGACAGAA GAAGCCGCAC AGTGTAACCC GGAGTTTCAA 
AATGAAGAAG TGGCTGGAGG AGAAAAAATA GATCCAGAAG AGGCACTGAT CTACGAAGAG 
GATTTAGATG AAGGAGATGG TGTTGAAGGA GAGTTGGAAG ACAGCACAAA ATTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCTTCCAAG CTTAAGGAGT TTATTGGCAA CATGATAACC 
ACCGCTGGAA AAGTTGTTGT GACAATTTTA CTGGGGTCAT CAGGTATGAT GCTGCCGTCT 
CTGACCTCGT CTGTGTACTT CTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACAGCA 
GGGCACTTAA TTGGACTTTA TTTATATCAG TTCCAATTCT TTCAAGAAGC GGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAATCAGTCA TTCAAACAGA CTGTTCCAGC 
ACGTGGAAGA TCATAGCGAA CCCAGACCTG TCCTGGTATC ACCACGCCAA CCCCATCCTC 
CTGCTGGTGA TGTACTACAC GCTGGCCACT CTCATCCGCA TCTGGCTACA GGAGCCGCTT 
GTGCAGGATG AGAGGACCAA GGAAGAGGAC AGAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACGGCCGAGA GGAGGCGGAG CCTGTGGTAC GCGACCCATT ACCCAACTGA CGAGAGAAAA 
GGTCTGCTGG TGACTGTGAA CGGCAATCCT GTGGATTACC ACACCATCCA CCCCAGTCTT 
CCCATGGAGA ACGGCCCCGC CAAAGCCGAC CTGTACTCCA CCCCGCAGTA CCGGTGGGAG 
CCCTCCGACG ACTCCTCGGA AAAGAAAGAG GAAGAAGAAG ATGAGAAAGA AGAATTTGAA 
GAGGAAAGGA GCCATGAGGA GAAAAGAAGT GTTAAAGTTC ACGCCATGGT CTCTGTGTTC 
CAGTTTATTA TGAAACAAAG TTACATCTGT GCCCTTATTG CTATGATGGC TTGGAGCATC 
ACCTATCACA GCTGGTTGAC CTTCGTGCTG TTGATTTGGT CGTGCACTCT TTGGATGATT 
CGCAACAGGA GAAAATATGC CATGATCAGC TCTCCTTTCA TGGTTGTTTA TGGAAATCTA 
CTGCTGATAT TACAGTATAT ATGGAGTTTT GAACTTCCTG AAATTAAAAA GGTCCCAGGA 
TTTTTAGAAA AGAAAGAGCC AGGAGAACTT GCCTCAAAGA TACTTTTCAC CATTACTTTT 
TGGCTACTGC TGAGGCAGCA CCTCACAGAG CAGAAAGCTC TTCGGGAAAA GGAAGCTCTT 
TTATCTGAGG TCAAAATTGG GAGTCAGGAA AATGAAGAAA AAGCTGATGA GCAAGATATA 
CAGGTGGAAG GAGAGGCGGA AGAGAAGGAG GAAGGAGAGG AAGCAAAGCG AGAGAAGCAG 
GAGATAAAGA AGGAAGAGGA AGAGGAAGAG GAAGTGGAGG AAGGTGACGA TCAGGACATC 
ATGACGGTGC TGGGCAATCT GGTGGTGGCC ATGTTCATTA AGTACTGGAT CTACGTCTGT 
GGAGGGATGT TCTTCTTCGT CAGCTTCGAG GGTAAAATTG TGATGTACAA AATCATCTAC 
ATGGTGCTGT TTCTATTCTG TGTGGCCTTG TATCAGGTGC ACTATGAATG GTGGAGGCGA 
ATTCTAAAAT ATTTTTGGAT GTCAGTGGTT ATTTATACTA TGCTGGTGCT CATCTTTATA 
TACACATATC AGTTTGAAAA CTTCCCAGGC CTATGGCAAA ATATGACTGG ATTAAAAAGA 
GAAAAGCTTG AGGATCTTGG CTTAAAGCAG TTTACCGTGG CTGAACTATT CACTCGCATA 
TTCATCCCAA CCTCCTTTCT CCTGGTGTGC ATTTTGCACC TTCACTATTT CCATGACCGG 
TTCCTGGAGC TCACAGACCT GAAGTCCATT CCCTGCAAGG AAGACAGCGC CATCTACAGA 
CTGGCGCACC CTGAGGGAAG CCTGCCGGAC CTCACCATGA TGAACCTGAC CGCCAGCCTG 
CAGGCGCCGG AGATGGGGAA GCCGGCAGAG CCTGGTGCGG AGAAGCTGGA GGGGTGCCCT 
GAGAAAGCCG AGAAGGCTGA GCTTGGGAAA GACAGTGAGG AGGAGGAGGA GGAGGAAGAG 
GAAGAGGAGG AGGCAGGGAC GTCAGATTTA AGGAACAAAT GGCACCTGGT GATTGACCGC 
CTTACTGTGC TCTTCTTAAA ATTCCTGGAG TATTTTCACA AGCTGCAGGT GTTCATGTGG 
TGGATTTTGG AGTTGCATAT CATCAAAATC GTTTCATCAT ACATTATCTG GGTTTCTGTG 
AAAGAGGTGT CTCTGTTCAA CTATGTATTT TTGATTTCTT GGGCTTTTGC TCTGCCATAT 
GCCAAGCTAC GCCGTCTGGC TTCAAGTGTC TGCACTGTCT GGACGTGTGT GATCATCGTC 
TGCAAAATGT TGTACCAGCT GCAAACCATT AAGCCTGAGA ACTTTTCTGT TAACTGTTCC 
TTGCCAAATG AAAACCAAAC GAATGTCCCC CTCCAGCAGT TGAACAAGTC ACTGCTCTAC 
AACGCTCCTA TTGACCCGAC AGAGTGGGTC GGCCTGAGGA AGTCCTCCCC TTTGCTGGTC 
TACCTGAGGA ATAACCTCCT GATGCTGGCC ATCCTGGCCT TCGAGGTTAC AATTTACCGC 
CATCAGGAAT ACTATCGAGG TCGGAACAAT CTCACGGCCC CCGTGTCTAA AACCATCTTT 
CACGACATTA CGAGGCTCCA TCTAGATGAT GGACTTATTA ATTGTGCCAA GTATTTCATA 
AATTACTTCT TCTACAAGTT TGGCCTAGAG ACCTGTTTCC TAATGTCAGT GAACGTCATT 
GGGCAGCGAA TGGACTTCTA TGCCATGATT CACGCCTGCT GGTTGATCGC TGTCTTATAT 
CGACGCAGAA GAAAAGCCAT TGCGGAGATC TGGCCCAAGT ACTGCTGCTT CCTGGCCTGC 
ATCATCACGT TCCAGTACTT CATCTGCATC GGCATTCCTC CTGCTCCTTG CAGAGATTAC 
CCGTGGAGAT TCAAGGGTGC CAGCTTCAAC GACAACATCA TTAAGTGGCT GTACTTCCCA 
GACTTCATTG TGCGGCCCAA CCCCGTGTTT CTCGTCTATG ACTTCATGCT GCTTCTGTGT 
GCCTCCTTAC AAAGGCAAAT TTTTGAGGAT GAAAATAAGG CTGCAGTGCG AATCATGGCG 
GGAGACAATG TCGAGATTTG CATGAACCTT GATGCAGCCT CTTTCAGCCA GCATAACCCT 
GTACCAGACT TCATTCACTG CAGGTCGTAC TTAGACATGT CCAAAGTAAT CATCTTCAGC 
TACCTCTTCT GGTTCGTCCT CACGATCATC TTCATCACTG GGACCACCAG GATCAGCATC 
TTTTGCATGG GTTACTTGGT GGCCTGCTTC TATTTCCTGC TTTTTGGGGG CAATTTGCTG 
TTGAAACCCA TCAAGAGCAT CCTGCGCTAT TGGGACTGGC TGATTGCATA CAACGTTTTT 
GTGATTACAA TGAAAAACAT ACTGTCAATA GGAGCATGTG GATACATTGA AAAATTGGTT 
CAAAATAGTT GCTGGTTGAT CCAAGCTTTC AGCCTGGCCT GCACCGTCAA AGGCTATAAA 
ATGCCTGATA ACGATTCCTC ATGTAAACTG CCCAGTGGTG AAGCAGGAAT TATCTGGGAC 
AGCATCTGTT TTGCCTTCCT CCTGCTGCAG AGGAGAGTTT TCATGAGTTA CTATTTTCTA 
CATGTCGTGG CAGATATAAA AGCTTCACAG ATCCTGGCAT CAAGAGGGGC AGAACTTTTC 
CAGGCCACAA TTGTGAAGGC TGTAAAGGCC AGAATTGAGG AGGAGAAGAA GTCAATGGAT 
CAGTTGAAGA GGCAGATGGA TCGCATCAAG GCCAGACAAC AGAAATACAA AAAGGGTAAG 
GAGAGGATGC TGAGCTTGAC TCAGGAGTCA GGGGAAGGCC AAGACATCCA AAAGCTCTCT 
GAAGAGGATG ATGAAAGAGA AGCAGACAAA CAGAAAGCCA AAGGCAAAAA AAAGCAGTGG 
TGGCGGCCTT GGGTCGATCA TGCTTCCATG GTCAGGAGTG GAGATTATTA TTTGTTTGAA 
ACGGATAGTG AAGAAGAGGA AGAGGAAGAA TTTAAAAAGG AAGAAGAAGA GCCTCCAAGA 
AGGTCGGCCT TCCAGTTTGT TTATCAAGCC TGGATTACTG ACCCTAAAAC AGCACTCCGT 
CAGAGGCGCA AAGAGAAAAA GAGATCTTCA AGAGAAGGCC AGAAACGAAG GAGAAAGGGA 
TCTGGGGAGG GTCCTGTGGA GTGGGAAGAT CGAGAGGATG AACCAGTCAA AAAGAAATCT 
GATGGCCCAG ATAATATCAT CAAGAGGATA TTTAATATTT TGAAATTTAC CTGGGTCCTG 
TTTCTGGCAA CGGTGGATAG TTTCACAACT TGGCTTAATT CCATTTCAAG GGAGCATATT 
GATATATCCA CAGTTCTGAG AATTGAAAGA TGCATGCTGA CCAGAGAAAT TAAAAAGGGC 
AACGTTCCCA CTCGGGAGAG CATCCACATG TACTATCAGA ACCACATCAT GAACCTTTCC 
CGAGAGTCTG GACTGGACAC CATCGACGAC CGTCCTGGTG CTGCTTCAGG TGCACAGACA 
GCCCACAGGA TGGATAGCTT AGATTCTCAT GACAGTATCT CCAGCGAGCC CACCCAGTGC 
ACCATTCTGT CCTCGCGCCA GGGGACCACG GAAACCATCG AGGAGGTGGA GACTGAGCAG 
GACGAGGAGG CCGAGGGGGA CGAGGAGGCC GGGGAGGCTG AGGAGGCGGG GGTCGGCGAC 
CCTGACGAGG CGGGTTTGGA GGCCCCAGAG GATGGCGAGG ATGGCGTCGA GGAGGCCCCG 
CCGTCGTACA GCAAGGCCGT CAGCCTGGAG CGCCTGTCCT TGGACTCGCC GGACGGCTCC 
GCAGGCCGGA ACCACATGGC GGTCAGCCCC GACGACAGCC GCTCCGACAG GCTGGAGTCC 
AGCATCTTAC CCCCGCTGAC TCACGAGCTG ACGGCCAGCG AGCTGCTGCT GAACAAGATG 
TTCCATGACG ATGAGCTGGA GGAGTCGGAG AAATTCTACG TTGGCCAGCC GCGGTTCTTG 
CTGCTCTTCT ACGCCATGTA CAACACCCTG GTGGCCCGCT CGGAAATGGT GTGCTATTTC 
GTCATCATCC TCAACCACAT GGTCTCTGCC TCCATGATCA CCCTCGTGCT TCCCATCCTG 
ATCTTCCTCT GGGCCATGCT GTCTGTCCCC AGGCCCAGCA AGCGGTTCTG GATGATGGCC 
ATTGTCTACA CAGAGGTGGC AATTGTGGTC AAGTATTTCT TCCAGTTTGG GTTCTTTCCC 
TGGAACAAGC ACGTGGAATT GAACAAAGAC AAACCTTACC ACCCGCCTAA TATCATAGGA 
GTGGAAAAGA AAGAAGGTTA TGTCCTTTAC GACCTCATTC AGCTCCTGGC TCTGTTCTTC 
CATCGATCGA TTCTGAAGTG CCACGGTTTA TGGGATGAAG ATGACGTCAC CGACAGTGGC 
TTAGACAAGG AAGAGTCAGA TGACGAGCTC TCCCTCAGTC ACCGCAGAAG AGCTTCCTCC 
GATTCTCTGA AATCCATCAA CCTGGCCGCA TCGGCGGAGT CCGTGCACGT GTCCTTCCCC 
GAGCAGCCGG CAGCCGTCCG CAGGAAGCGC TCCGCCAGCA GCTCCCAGAT ATCCCAGAGA 
TCCAGCTTTT CTTCAAACAG GTCCAAAAGA GGCAGCACAA GCACCCGAAA CAGCAGTCAA 
AAAGGAAGCA GTGTTTTGAG CATTAAGCAA AAAAGCAAAA GGGAGCTTTA TATGGAAAAG 
CTTCAAGAAC ATTTAATCAA AGCAAAAGCA TTTACTATAA AGAAGACTCT GCAAATATAT 
GTGCCCATCA GACAGTTCTT CTACAACCTC ATCCACCCCG ACTACAGCGC CGTGACCGAT 
GTGTATGTGC TCATGTTCCT GGCCGACACT GTCGACTTTG TCATCATTGT CTTTGGCTTC 
TGGGCCTTTG GGAAACACTC AGCAGCTGCA GACATCACCT CTTCGCTGTC CGAGGACCAG 
GTCCCTGGGC CGTTTTTGGT GATGGTCCTC ATCCAGTTTG GAACCATGGT GGTGGACCGA 
GCACTGTACC TCAGGAAGAC TGTATTGGGA AAGGTCATTT TCCAGGTCAT TCTTGTGTTT 
GGGATTCACT TCTGGATGTT CTTCATCTTG CCTGGCGTGA CGGAGAGGAA ATTCAGCCAG 
AACCTGGTTG CTCAGCTTTG GTACTTTGTG AAGTGTGTTT ACTTCGGGCT GTCTGCCTAC 
CAAATCCGTT GTGGCTACCC AACTCGTGTC CTTGGAAACT TCCTCACAAA GAGCTACAAT 
TATGTCAACC TCTTCTTGTT TCAAGGGTTC CGCCTTGTTC CGTTTTTGAC TGAGCTGAGG 
GCCGTGATGG ACTGGGTGTG GACAGACACG ACCTTGAGCC TCTCCAGCTG GATCTGTGTA 
GAGGACATCT ATGCTCACAT ATTCATCCTG AAGTGTTGGC GGGAGTCAGA AAAAAGATAC 
CCTCAGCCTC GGGGGCAGAA AAAGAAGAAG GTGGTAAAGT ATGGCATGGG GGGCATGATC 
ATCGTGCTGC TCATCTGCAT CGTCTGGTTT CCTCTTCTCT TCATGTCCCT GATCAAGTCT 
GTCGCTGGAG TGATCAACCA GCCCCTGGAT GTCTCCGTCA CAATCACCCT GGGAGGGTAC 
CAGCCTATTT TCACGATGAG TGCCCAGCAA AGCCAGTTGA AAGTTATGGA CCAGCCAAAG 
TTTAATAAAT TTATAAAAGC TTTTTCTAGG GACACTGGTG CTATGCAATT TCTGGAAAAT 
TATGAAAAAG AAGACATAAC AGTAGCAGAA CTGGAAGGAA ACTCAAATTC TTTGTGGACC 
ATCAGCCCTC CCAGTAAGCA GAAAATGATA CACGAACTCA CGGACCCCAA TAGTAGCTTC 
TCTGTTGTTT TTTCCTGGAG TATTCAGAGA AACATGAGTC TGGGTGCAAA AGCAGAAATA 
GCAACAGATA AGCTTTCCTT TCCTCTTAAA AATACTACCC GAGAGAATAT AGCTAAAATG 
ATAGCTGGCA ACAACACAGA AAGTTCCAAA ACACCTGTGA CTATAGAAAA GATCTACCCG 
TATTACGTGA AAGCACCCAG TGATTCCAAC TCAAAACCTA TAAAGCAACT GTTATCTGAA 
AATAATTTCA TGAACATCAC CATCATTTTG TCAAGAGATA CTTCAACTAA ATCTAACAGT 
GAGTGGTGGG TTCTCAACCT GACTGGAAAC AGAATATACA ACCAGCATGC TCAGGCCTTG 
GAGCTGGTGG TCTTCAATGA CAAAGTCAGC CCCCCTAGCC TGGGGTTCCT GGCTGGCTAC 
GGTATCATGG GACTATATGC TTCGGTTGTC CTCGTGATTG GGAAGTTTGT CCGTGAATTC 
TTCAGCGGGA TTTCTCACTC CATCATGTTT GAGGAGCTTC CAAATGTCGA TCGAATTTTG 
AAGTTATGCA CGGATATTTT TTTGGTTCGA GAGACAGGGG AACTGGAACT AGAGGAAGAT 
CTCTATGCCA AATTAATATT CCTGTATCGT TCACCAGAAA CCATGATCAA ATGGACCAGG 
GAAAAAACAA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023503240.1
CDS	40..8214
Translation	MASEVVCGLIFRLLLPICLAVACAFRYNGLSFVYLIYLLLIPLFSEPTKATMQGHTGRLLKSLCFTSLSFLLLHVIFHITLASLEAQHRIAPGYNCSTWEKTLRQIGFESLKGADAGNGIRVFVPDIGMFIASLTIWLVCRNIVQKPVTEEAAQCNPEFQNEEVAGGEKIDPEEALIYEEDLDEGDGVEGELEDSTKLKMFRRLASVASKLKEFIGNMITTAGKVVVTILLGSSGMMLPSLTSSVYFFVFLGLCTWWSWCRTFDPLLFSCLCVLLAIFTAGHLIGLYLYQFQFFQEAVPPNDYYARLFGIKSVIQTDCSSTWKIIANPDLSWYHHANPILLLVMYYTLATLIRIWLQEPLVQDERTKEEDRALACSPIQITAERRRSLWYATHYPTDERKGLLVTVNGNPVDYHTIHPSLPMENGPAKADLYSTPQYRWEPSDDSSEKKEEEEDEKEEFEEERSHEEKRSVKVHAMVSVFQFIMKQSYICALIAMMAWSITYHSWLTFVLLIWSCTLWMIRNRRKYAMISSPFMVVYGNLLLILQYIWSFELPEIKKVPGFLEKKEPGELASKILFTITFWLLLRQHLTEQKALREKEALLSEVKIGSQENEEKADEQDIQVEGEAEEKEEGEEAKREKQEIKKEEEEEEEVEEGDDQDIMTVLGNLVVAMFIKYWIYVCGGMFFFVSFEGKIVMYKIIYMVLFLFCVALYQVHYEWWRRILKYFWMSVVIYTMLVLIFIYTYQFENFPGLWQNMTGLKREKLEDLGLKQFTVAELFTRIFIPTSFLLVCILHLHYFHDRFLELTDLKSIPCKEDSAIYRLAHPEGSLPDLTMMNLTASLQAPEMGKPAEPGAEKLEGCPEKAEKAELGKDSEEEEEEEEEEEEAGTSDLRNKWHLVIDRLTVLFLKFLEYFHKLQVFMWWILELHIIKIVSSYIIWVSVKEVSLFNYVFLISWAFALPYAKLRRLASSVCTVWTCVIIVCKMLYQLQTIKPENFSVNCSLPNENQTNVPLQQLNKSLLYNAPIDPTEWVGLRKSSPLLVYLRNNLLMLAILAFEVTIYRHQEYYRGRNNLTAPVSKTIFHDITRLHLDDGLINCAKYFINYFFYKFGLETCFLMSVNVIGQRMDFYAMIHACWLIAVLYRRRRKAIAEIWPKYCCFLACIITFQYFICIGIPPAPCRDYPWRFKGASFNDNIIKWLYFPDFIVRPNPVFLVYDFMLLLCASLQRQIFEDENKAAVRIMAGDNVEICMNLDAASFSQHNPVPDFIHCRSYLDMSKVIIFSYLFWFVLTIIFITGTTRISIFCMGYLVACFYFLLFGGNLLLKPIKSILRYWDWLIAYNVFVITMKNILSIGACGYIEKLVQNSCWLIQAFSLACTVKGYKMPDNDSSCKLPSGEAGIIWDSICFAFLLLQRRVFMSYYFLHVVADIKASQILASRGAELFQATIVKAVKARIEEEKKSMDQLKRQMDRIKARQQKYKKGKERMLSLTQESGEGQDIQKLSEEDDEREADKQKAKGKKKQWWRPWVDHASMVRSGDYYLFETDSEEEEEEEFKKEEEEPPRRSAFQFVYQAWITDPKTALRQRRKEKKRSSREGQKRRRKGSGEGPVEWEDREDEPVKKKSDGPDNIIKRIFNILKFTWVLFLATVDSFTTWLNSISREHIDISTVLRIERCMLTREIKKGNVPTRESIHMYYQNHIMNLSRESGLDTIDDRPGAASGAQTAHRMDSLDSHDSISSEPTQCTILSSRQGTTETIEEVETEQDEEAEGDEEAGEAEEAGVGDPDEAGLEAPEDGEDGVEEAPPSYSKAVSLERLSLDSPDGSAGRNHMAVSPDDSRSDRLESSILPPLTHELTASELLLNKMFHDDELEESEKFYVGQPRFLLLFYAMYNTLVARSEMVCYFVIILNHMVSASMITLVLPILIFLWAMLSVPRPSKRFWMMAIVYTEVAIVVKYFFQFGFFPWNKHVELNKDKPYHPPNIIGVEKKEGYVLYDLIQLLALFFHRSILKCHGLWDEDDVTDSGLDKEESDDELSLSHRRRASSDSLKSINLAASAESVHVSFPEQPAAVRRKRSASSSQISQRSSFSSNRSKRGSTSTRNSSQKGSSVLSIKQKSKRELYMEKLQEHLIKAKAFTIKKTLQIYVPIRQFFYNLIHPDYSAVTDVYVLMFLADTVDFVIIVFGFWAFGKHSAAADITSSLSEDQVPGPFLVMVLIQFGTMVVDRALYLRKTVLGKVIFQVILVFGIHFWMFFILPGVTERKFSQNLVAQLWYFVKCVYFGLSAYQIRCGYPTRVLGNFLTKSYNYVNLFLFQGFRLVPFLTELRAVMDWVWTDTTLSLSSWICVEDIYAHIFILKCWRESEKRYPQPRGQKKKKVVKYGMGGMIIVLLICIVWFPLLFMSLIKSVAGVINQPLDVSVTITLGGYQPIFTMSAQQSQLKVMDQPKFNKFIKAFSRDTGAMQFLENYEKEDITVAELEGNSNSLWTISPPSKQKMIHELTDPNSSFSVVFSWSIQRNMSLGAKAEIATDKLSFPLKNTTRENIAKMIAGNNTESSKTPVTIEKIYPYYVKAPSDSNSKPIKQLLSENNFMNITIILSRDTSTKSNSEWWVLNLTGNRIYNQHAQALELVVFNDKVSPPSLGFLAGYGIMGLYASVVLVIGKFVREFFSGISHSIMFEELPNVDRILKLCTDIFLVRETGELELEEDLYAKLIFLYRSPETMIKWTREKTN

Target ORF information:

RefSeq Version	XM_023647472.1
Organism	Equus caballus(horse)
Definition	Equus caballus piezo type mechanosensitive ion channel component 2 (PIEZO2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023647472.1

ORF Insert Sequence:

ATGGCCTCGG AAGTGGTGTG CGGACTCATC TTCAGGCTGC TGCTCCCCAT CTGCCTGGCA 
GTAGCGTGTG CATTCCGATA CAATGGGCTC TCCTTTGTCT ACCTCATCTA CCTCTTGCTC 
ATCCCTCTGT TCTCAGAACC AACAAAAGCG ACGATGCAAG GACATACGGG ACGGTTGTTG 
AAGTCTCTGT GCTTCACCAG TCTGTCCTTC CTGTTGCTGC ACGTCATCTT TCACATCACA 
TTGGCCAGCC TGGAAGCTCA GCACCGTATC GCGCCTGGTT ACAACTGCTC GACGTGGGAA 
AAGACGCTTC GGCAGATTGG CTTTGAAAGC TTAAAGGGAG CCGACGCTGG CAATGGGATC 
AGAGTGTTTG TACCTGACAT CGGGATGTTC ATTGCTAGTC TGACCATCTG GCTCGTCTGT 
AGGAACATTG TTCAGAAACC GGTGACAGAA GAAGCCGCAC AGTGTAACCC GGAGTTTCAA 
AATGAAGAAG TGGCTGGAGG AGAAAAAATA GATCCAGAAG AGGCACTGAT CTACGAAGAG 
GATTTAGATG AAGGAGATGG TGTTGAAGGA GAGTTGGAAG ACAGCACAAA ATTAAAAATG 
TTCCGCAGGC TTGCCTCTGT GGCTTCCAAG CTTAAGGAGT TTATTGGCAA CATGATAACC 
ACCGCTGGAA AAGTTGTTGT GACAATTTTA CTGGGGTCAT CAGGTATGAT GCTGCCGTCT 
CTGACCTCGT CTGTGTACTT CTTTGTATTT TTGGGTCTGT GCACCTGGTG GTCCTGGTGC 
CGGACGTTCG ACCCATTGCT GTTCAGCTGT CTCTGTGTTC TGCTGGCTAT TTTCACAGCA 
GGGCACTTAA TTGGACTTTA TTTATATCAG TTCCAATTCT TTCAAGAAGC GGTTCCACCC 
AATGACTACT ATGCAAGGTT GTTTGGTATC AAATCAGTCA TTCAAACAGA CTGTTCCAGC 
ACGTGGAAGA TCATAGCGAA CCCAGACCTG TCCTGGTATC ACCACGCCAA CCCCATCCTC 
CTGCTGGTGA TGTACTACAC GCTGGCCACT CTCATCCGCA TCTGGCTACA GGAGCCGCTT 
GTGCAGGATG AGAGGACCAA GGAAGAGGAC AGAGCCCTGG CTTGTAGCCC CATCCAAATA 
ACGGCCGAGA GGAGGCGGAG CCTGTGGTAC GCGACCCATT ACCCAACTGA CGAGAGAAAA 
GGTCTGCTGG TGACTGTGAA CGGCAATCCT GTGGATTACC ACACCATCCA CCCCAGTCTT 
CCCATGGAGA ACGGCCCCGC CAAAGCCGAC CTGTACTCCA CCCCGCAGTA CCGGTGGGAG 
CCCTCCGACG ACTCCTCGGA AAAGAAAGAG GAAGAAGAAG ATGAGAAAGA AGAATTTGAA 
GAGGAAAGGA GCCATGAGGA GAAAAGAAGT GTTAAAGTTC ACGCCATGGT CTCTGTGTTC 
CAGTTTATTA TGAAACAAAG TTACATCTGT GCCCTTATTG CTATGATGGC TTGGAGCATC 
ACCTATCACA GCTGGTTGAC CTTCGTGCTG TTGATTTGGT CGTGCACTCT TTGGATGATT 
CGCAACAGGA GAAAATATGC CATGATCAGC TCTCCTTTCA TGGTTGTTTA TGGAAATCTA 
CTGCTGATAT TACAGTATAT ATGGAGTTTT GAACTTCCTG AAATTAAAAA GGTCCCAGGA 
TTTTTAGAAA AGAAAGAGCC AGGAGAACTT GCCTCAAAGA TACTTTTCAC CATTACTTTT 
TGGCTACTGC TGAGGCAGCA CCTCACAGAG CAGAAAGCTC TTCGGGAAAA GGAAGCTCTT 
TTATCTGAGG TCAAAATTGG GAGTCAGGAA AATGAAGAAA AAGCTGATGA GCAAGATATA 
CAGGTGGAAG GAGAGGCGGA AGAGAAGGAG GAAGGAGAGG AAGCAAAGCG AGAGAAGCAG 
GAGATAAAGA AGGAAGAGGA AGAGGAAGAG GAAGTGGAGG AAGGTGACGA TCAGGACATC 
ATGACGGTGC TGGGCAATCT GGTGGTGGCC ATGTTCATTA AGTACTGGAT CTACGTCTGT 
GGAGGGATGT TCTTCTTCGT CAGCTTCGAG GGTAAAATTG TGATGTACAA AATCATCTAC 
ATGGTGCTGT TTCTATTCTG TGTGGCCTTG TATCAGGTGC ACTATGAATG GTGGAGGCGA 
ATTCTAAAAT ATTTTTGGAT GTCAGTGGTT ATTTATACTA TGCTGGTGCT CATCTTTATA 
TACACATATC AGTTTGAAAA CTTCCCAGGC CTATGGCAAA ATATGACTGG ATTAAAAAGA 
GAAAAGCTTG AGGATCTTGG CTTAAAGCAG TTTACCGTGG CTGAACTATT CACTCGCATA 
TTCATCCCAA CCTCCTTTCT CCTGGTGTGC ATTTTGCACC TTCACTATTT CCATGACCGG 
TTCCTGGAGC TCACAGACCT GAAGTCCATT CCCTGCAAGG AAGACAGCGC CATCTACAGA 
CTGGCGCACC CTGAGGGAAG CCTGCCGGAC CTCACCATGA TGAACCTGAC CGCCAGCCTG 
CAGGCGCCGG AGATGGGGAA GCCGGCAGAG CCTGGTGCGG AGAAGCTGGA GGGGTGCCCT 
GAGAAAGCCG AGAAGGCTGA GCTTGGGAAA GACAGTGAGG AGGAGGAGGA GGAGGAAGAG 
GAAGAGGAGG AGGCAGGGAC GTCAGATTTA AGGAACAAAT GGCACCTGGT GATTGACCGC 
CTTACTGTGC TCTTCTTAAA ATTCCTGGAG TATTTTCACA AGCTGCAGGT GTTCATGTGG 
TGGATTTTGG AGTTGCATAT CATCAAAATC GTTTCATCAT ACATTATCTG GGTTTCTGTG 
AAAGAGGTGT CTCTGTTCAA CTATGTATTT TTGATTTCTT GGGCTTTTGC TCTGCCATAT 
GCCAAGCTAC GCCGTCTGGC TTCAAGTGTC TGCACTGTCT GGACGTGTGT GATCATCGTC 
TGCAAAATGT TGTACCAGCT GCAAACCATT AAGCCTGAGA ACTTTTCTGT TAACTGTTCC 
TTGCCAAATG AAAACCAAAC GAATGTCCCC CTCCAGCAGT TGAACAAGTC ACTGCTCTAC 
AACGCTCCTA TTGACCCGAC AGAGTGGGTC GGCCTGAGGA AGTCCTCCCC TTTGCTGGTC 
TACCTGAGGA ATAACCTCCT GATGCTGGCC ATCCTGGCCT TCGAGGTTAC AATTTACCGC 
CATCAGGAAT ACTATCGAGG TCGGAACAAT CTCACGGCCC CCGTGTCTAA AACCATCTTT 
CACGACATTA CGAGGCTCCA TCTAGATGAT GGACTTATTA ATTGTGCCAA GTATTTCATA 
AATTACTTCT TCTACAAGTT TGGCCTAGAG ACCTGTTTCC TAATGTCAGT GAACGTCATT 
GGGCAGCGAA TGGACTTCTA TGCCATGATT CACGCCTGCT GGTTGATCGC TGTCTTATAT 
CGACGCAGAA GAAAAGCCAT TGCGGAGATC TGGCCCAAGT ACTGCTGCTT CCTGGCCTGC 
ATCATCACGT TCCAGTACTT CATCTGCATC GGCATTCCTC CTGCTCCTTG CAGAGATTAC 
CCGTGGAGAT TCAAGGGTGC CAGCTTCAAC GACAACATCA TTAAGTGGCT GTACTTCCCA 
GACTTCATTG TGCGGCCCAA CCCCGTGTTT CTCGTCTATG ACTTCATGCT GCTTCTGTGT 
GCCTCCTTAC AAAGGCAAAT TTTTGAGGAT GAAAATAAGG CTGCAGTGCG AATCATGGCG 
GGAGACAATG TCGAGATTTG CATGAACCTT GATGCAGCCT CTTTCAGCCA GCATAACCCT 
GTACCAGACT TCATTCACTG CAGGTCGTAC TTAGACATGT CCAAAGTAAT CATCTTCAGC 
TACCTCTTCT GGTTCGTCCT CACGATCATC TTCATCACTG GGACCACCAG GATCAGCATC 
TTTTGCATGG GTTACTTGGT GGCCTGCTTC TATTTCCTGC TTTTTGGGGG CAATTTGCTG 
TTGAAACCCA TCAAGAGCAT CCTGCGCTAT TGGGACTGGC TGATTGCATA CAACGTTTTT 
GTGATTACAA TGAAAAACAT ACTGTCAATA GGAGCATGTG GATACATTGA AAAATTGGTT 
CAAAATAGTT GCTGGTTGAT CCAAGCTTTC AGCCTGGCCT GCACCGTCAA AGGCTATAAA 
ATGCCTGATA ACGATTCCTC ATGTAAACTG CCCAGTGGTG AAGCAGGAAT TATCTGGGAC 
AGCATCTGTT TTGCCTTCCT CCTGCTGCAG AGGAGAGTTT TCATGAGTTA CTATTTTCTA 
CATGTCGTGG CAGATATAAA AGCTTCACAG ATCCTGGCAT CAAGAGGGGC AGAACTTTTC 
CAGGCCACAA TTGTGAAGGC TGTAAAGGCC AGAATTGAGG AGGAGAAGAA GTCAATGGAT 
CAGTTGAAGA GGCAGATGGA TCGCATCAAG GCCAGACAAC AGAAATACAA AAAGGGTAAG 
GAGAGGATGC TGAGCTTGAC TCAGGAGTCA GGGGAAGGCC AAGACATCCA AAAGCTCTCT 
GAAGAGGATG ATGAAAGAGA AGCAGACAAA CAGAAAGCCA AAGGCAAAAA AAAGCAGTGG 
TGGCGGCCTT GGGTCGATCA TGCTTCCATG GTCAGGAGTG GAGATTATTA TTTGTTTGAA 
ACGGATAGTG AAGAAGAGGA AGAGGAAGAA TTTAAAAAGG AAGAAGAAGA GCCTCCAAGA 
AGGTCGGCCT TCCAGTTTGT TTATCAAGCC TGGATTACTG ACCCTAAAAC AGCACTCCGT 
CAGAGGCGCA AAGAGAAAAA GAGATCTTCA AGAGAAGGCC AGAAACGAAG GAGAAAGGGA 
TCTGGGGAGG GTCCTGTGGA GTGGGAAGAT CGAGAGGATG AACCAGTCAA AAAGAAATCT 
GATGGCCCAG ATAATATCAT CAAGAGGATA TTTAATATTT TGAAATTTAC CTGGGTCCTG 
TTTCTGGCAA CGGTGGATAG TTTCACAACT TGGCTTAATT CCATTTCAAG GGAGCATATT 
GATATATCCA CAGTTCTGAG AATTGAAAGA TGCATGCTGA CCAGAGAAAT TAAAAAGGGC 
AACGTTCCCA CTCGGGAGAG CATCCACATG TACTATCAGA ACCACATCAT GAACCTTTCC 
CGAGAGTCTG GACTGGACAC CATCGACGAC CGTCCTGGTG CTGCTTCAGG TGCACAGACA 
GCCCACAGGA TGGATAGCTT AGATTCTCAT GACAGTATCT CCAGCGAGCC CACCCAGTGC 
ACCATTCTGT CCTCGCGCCA GGGGACCACG GAAACCATCG AGGAGGTGGA GACTGAGCAG 
GACGAGGAGG CCGAGGGGGA CGAGGAGGCC GGGGAGGCTG AGGAGGCGGG GGTCGGCGAC 
CCTGACGAGG CGGGTTTGGA GGCCCCAGAG GATGGCGAGG ATGGCGTCGA GGAGGCCCCG 
CCGTCGTACA GCAAGGCCGT CAGCCTGGAG CGCCTGTCCT TGGACTCGCC GGACGGCTCC 
GCAGGCCGGA ACCACATGGC GGTCAGCCCC GACGACAGCC GCTCCGACAG GCTGGAGTCC 
AGCATCTTAC CCCCGCTGAC TCACGAGCTG ACGGCCAGCG AGCTGCTGCT GAACAAGATG 
TTCCATGACG ATGAGCTGGA GGAGTCGGAG AAATTCTACG TTGGCCAGCC GCGGTTCTTG 
CTGCTCTTCT ACGCCATGTA CAACACCCTG GTGGCCCGCT CGGAAATGGT GTGCTATTTC 
GTCATCATCC TCAACCACAT GGTCTCTGCC TCCATGATCA CCCTCGTGCT TCCCATCCTG 
ATCTTCCTCT GGGCCATGCT GTCTGTCCCC AGGCCCAGCA AGCGGTTCTG GATGATGGCC 
ATTGTCTACA CAGAGGTGGC AATTGTGGTC AAGTATTTCT TCCAGTTTGG GTTCTTTCCC 
TGGAACAAGC ACGTGGAATT GAACAAAGAC AAACCTTACC ACCCGCCTAA TATCATAGGA 
GTGGAAAAGA AAGAAGGTTA TGTCCTTTAC GACCTCATTC AGCTCCTGGC TCTGTTCTTC 
CATCGATCGA TTCTGAAGTG CCACGGTTTA TGGGATGAAG ATGACGTCAC CGACAGTGGC 
TTAGACAAGG AAGAGTCAGA TGACGAGCTC TCCCTCAGTC ACCGCAGAAG AGCTTCCTCC 
GATTCTCTGA AATCCATCAA CCTGGCCGCA TCGGCGGAGT CCGTGCACGT GTCCTTCCCC 
GAGCAGCCGG CAGCCGTCCG CAGGAAGCGC TCCGCCAGCA GCTCCCAGAT ATCCCAGAGA 
TCCAGCTTTT CTTCAAACAG GTCCAAAAGA GGCAGCACAA GCACCCGAAA CAGCAGTCAA 
AAAGGAAGCA GTGTTTTGAG CATTAAGCAA AAAAGCAAAA GGGAGCTTTA TATGGAAAAG 
CTTCAAGAAC ATTTAATCAA AGCAAAAGCA TTTACTATAA AGAAGACTCT GCAAATATAT 
GTGCCCATCA GACAGTTCTT CTACAACCTC ATCCACCCCG ACTACAGCGC CGTGACCGAT 
GTGTATGTGC TCATGTTCCT GGCCGACACT GTCGACTTTG TCATCATTGT CTTTGGCTTC 
TGGGCCTTTG GGAAACACTC AGCAGCTGCA GACATCACCT CTTCGCTGTC CGAGGACCAG 
GTCCCTGGGC CGTTTTTGGT GATGGTCCTC ATCCAGTTTG GAACCATGGT GGTGGACCGA 
GCACTGTACC TCAGGAAGAC TGTATTGGGA AAGGTCATTT TCCAGGTCAT TCTTGTGTTT 
GGGATTCACT TCTGGATGTT CTTCATCTTG CCTGGCGTGA CGGAGAGGAA ATTCAGCCAG 
AACCTGGTTG CTCAGCTTTG GTACTTTGTG AAGTGTGTTT ACTTCGGGCT GTCTGCCTAC 
CAAATCCGTT GTGGCTACCC AACTCGTGTC CTTGGAAACT TCCTCACAAA GAGCTACAAT 
TATGTCAACC TCTTCTTGTT TCAAGGGTTC CGCCTTGTTC CGTTTTTGAC TGAGCTGAGG 
GCCGTGATGG ACTGGGTGTG GACAGACACG ACCTTGAGCC TCTCCAGCTG GATCTGTGTA 
GAGGACATCT ATGCTCACAT ATTCATCCTG AAGTGTTGGC GGGAGTCAGA AAAAAGATAC 
CCTCAGCCTC GGGGGCAGAA AAAGAAGAAG GTGGTAAAGT ATGGCATGGG GGGCATGATC 
ATCGTGCTGC TCATCTGCAT CGTCTGGTTT CCTCTTCTCT TCATGTCCCT GATCAAGTCT 
GTCGCTGGAG TGATCAACCA GCCCCTGGAT GTCTCCGTCA CAATCACCCT GGGAGGGTAC 
CAGCCTATTT TCACGATGAG TGCCCAGCAA AGCCAGTTGA AAGTTATGGA CCAGCCAAAG 
TTTAATAAAT TTATAAAAGC TTTTTCTAGG GACACTGGTG CTATGCAATT TCTGGAAAAT 
TATGAAAAAG AAGACATAAC AGTAGCAGAA CTGGAAGGAA ACTCAAATTC TTTGTGGACC 
ATCAGCCCTC CCAGTAAGCA GAAAATGATA CACGAACTCA CGGACCCCAA TAGTAGCTTC 
TCTGTTGTTT TTTCCTGGAG TATTCAGAGA AACATGAGTC TGGGTGCAAA AGCAGAAATA 
GCAACAGATA AGCTTTCCTT TCCTCTTAAA AATACTACCC GAGAGAATAT AGCTAAAATG 
ATAGCTGGCA ACAACACAGA AAGTTCCAAA ACACCTGTGA CTATAGAAAA GATCTACCCG 
TATTACGTGA AAGCACCCAG TGATTCCAAC TCAAAACCTA TAAAGCAACT GTTATCTGAA 
AATAATTTCA TGAACATCAC CATCATTTTG TCAAGAGATA CTTCAACTAA ATCTAACAGT 
GAGTGGTGGG TTCTCAACCT GACTGGAAAC AGAATATACA ACCAGCATGC TCAGGCCTTG 
GAGCTGGTGG TCTTCAATGA CAAAGTCAGC CCCCCTAGCC TGGGGTTCCT GGCTGGCTAC 
GGTATCATGG GACTATATGC TTCGGTTGTC CTCGTGATTG GGAAGTTTGT CCGTGAATTC 
TTCAGCGGGA TTTCTCACTC CATCATGTTT GAGGAGCTTC CAAATGTCGA TCGAATTTTG 
AAGTTATGCA CGGATATTTT TTTGGTTCGA GAGACAGGGG AACTGGAACT AGAGGAAGAT 
CTCTATGCCA AATTAATATT CCTGTATCGT TCACCAGAAA CCATGATCAA ATGGACCAGG 
GAAAAAACAA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PIEZO2 cDNA ORF clone, Equus caballus(horse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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