Dpse\GA27534 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA27534
Entrez Gene ID	6901179
Full Name	GA27534 gene product from transcript GA27534-RA
Synonyms	GA27534,dpse_GLEANR_636
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_015185957.1	XP_015041443.1	uncharacterized protein, isoform B
XM_002133844.2	XP_002133880.2	uncharacterized protein, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA27534 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA27534 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe24298	XM_002133844.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA27534), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODe24649	XM_015185957.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA27534), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA27534 ( XM_002133844.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_002133880.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA27534), mRNA.

Price & Availability↑

CloneID	ODe24298
Clone ID Related Accession (Same CDS sequence)	XM_002133844.2
Accession Version	XM_002133844.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589960). On Dec 22, 2015 this sequence version replaced XM_002133844.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGAAAA ACTTCGGCCT TTTGCTGGGC CTGTTCGTCT TACTATTTGG CAGCCACGCA 
ACGAGTACCC ACGCAACAGC CGGCAATGAG AGCAACTTTC TGGAGGCATC CCTGAGATAC 
TTTGATGAGC AGCTGCTCTA CGACATACTG CCACGTGCCT CGTGCCCGGG CGACGATGAG 
ACCTCACCGA TGGATGAGTT TTCTGACCTC TTTACAGTGG AACAGCTTCG ACAGGGCTGG 
GTGGTGCTTC ATGTCTTTGC TGCGGTATAC TTCTTTATAC TGCTGGCCAT AATATGCAAC 
GACTACTTCC TGCCCACGGT GGAGTGCATC TGCGAGGACC TGCACCTGTC CAAGGACGTG 
GCGGCGGCCA CCTTTATGGC CACGGCCACA TCGATGCCGG AGTTCTTCAC CAACACTATC 
AGCACCCTGA TCCTGGAGTC GGACATGGGT CTGGGTACGA TCATCGGCTC GCTCATGTTC 
AACACCCTGG GCGTGGCGGG CCTGGCGGCG CTGTCCATTG ACAAGCCGGT TCAGCTGGAC 
TGGTGGCCAA TAGCGAGGGA CTGCTTCATC TACATATTCA ACACGATTAT CCTGCTGGTA 
CTGGCCTGGG GCGGCAGCAT TAGCTTCGTC GAGTCCTGCA TCATGATGGG CTTCCTAGTG 
CTCTACTATC TGATCACCTT CAACAACAAC AAGTTCATGC CAGCCATTCG CGTATTCATC 
GAGGATCGCA TGAACTGCTG CTTCTCCACG CGTTACGATC TAACGGAACC TCCGGAGAAC 
AGCGCCAAGG CACAGCTTCC GCTGAAGAAG GATCCCCTGA CGGGCGATGG CCTGTTCGTG 
GTAAACTTGC CAGAGAACAC CTCGTCGATG TCATCCACCG CCAATCTGAC TCACTCCAAG 
CATTGGAACG GCGATGACAG CGATGAAGAC GATATGCCAA AAAGCATCTA CGCCTATCCT 
CACGACGCCT CGCGCTTCAT GAAGAGCTGG TGGATCTTCG TTTTCCCGAT TAAGTTCACC 
CTCTCTTGGC TGATCCCTCA TCCGATGAAG CACCGCCGCC TCTACCCACT GTCCTTCATC 
ATGTGCATCC TCTGCATCGG GGGCAATGCC TACCTGATTG TCTGGATGCT GACGGCTTTC 
GGAGTGGCCA TCCATGTGCC AACAATAGTA ATGGGTCTCA CTTTCCTGGC TGCCGGCTCC 
ACCATGCCCG AGGCCGTCTC CAGTCTGATA TCCCTGAGGA ACGGTGAAAG CGGAATCGGA 
GTCTCGAACT CGCTGGGCGC CAACTCGCTG GCTATCCTGC TTTCTCTTGG CGTGCCCTGG 
TTCATCAAGA ACTGCCTAAA CTATGGAAGT GGGGAGCCGC AACAGGTCGG CACCCAGGGC 
ATCGAGTACA ATATCCTGAT ACTAATCATT TCAACGGTGG CCCTCTTCAT AATACTCAGC 
TTCAGTGGCT ACCGCTTGAC CAAGCGGGTG GGCATCGCTC TCTTCACCGT CTACGGAGTG 
TTTATCGTTC TTCAGATTCT TATTGAGATG AATGTGTTCT TCCCCAGGGA CTGCAGCAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002133880.2
CDS	291..1853
Translation	MVKNFGLLLGLFVLLFGSHATSTHATAGNESNFLEASLRYFDEQLLYDILPRASCPGDDETSPMDEFSDLFTVEQLRQGWVVLHVFAAVYFFILLAIICNDYFLPTVECICEDLHLSKDVAAATFMATATSMPEFFTNTISTLILESDMGLGTIIGSLMFNTLGVAGLAALSIDKPVQLDWWPIARDCFIYIFNTIILLVLAWGGSISFVESCIMMGFLVLYYLITFNNNKFMPAIRVFIEDRMNCCFSTRYDLTEPPENSAKAQLPLKKDPLTGDGLFVVNLPENTSSMSSTANLTHSKHWNGDDSDEDDMPKSIYAYPHDASRFMKSWWIFVFPIKFTLSWLIPHPMKHRRLYPLSFIMCILCIGGNAYLIVWMLTAFGVAIHVPTIVMGLTFLAAGSTMPEAVSSLISLRNGESGIGVSNSLGANSLAILLSLGVPWFIKNCLNYGSGEPQQVGTQGIEYNILILIISTVALFIILSFSGYRLTKRVGIALFTVYGVFIVLQILIEMNVFFPRDCSS

Target ORF information:

RefSeq Version	XM_002133844.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA27534), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002133844.2

ORF Insert Sequence:

ATGGTGAAAA ACTTCGGCCT TTTGCTGGGC CTGTTCGTCT TACTATTTGG CAGCCACGCA 
ACGAGTACCC ACGCAACAGC CGGCAATGAG AGCAACTTTC TGGAGGCATC CCTGAGATAC 
TTTGATGAGC AGCTGCTCTA CGACATACTG CCACGTGCCT CGTGCCCGGG CGACGATGAG 
ACCTCACCGA TGGATGAGTT TTCTGACCTC TTTACAGTGG AACAGCTTCG ACAGGGCTGG 
GTGGTGCTTC ATGTCTTTGC TGCGGTATAC TTCTTTATAC TGCTGGCCAT AATATGCAAC 
GACTACTTCC TGCCCACGGT GGAGTGCATC TGCGAGGACC TGCACCTGTC CAAGGACGTG 
GCGGCGGCCA CCTTTATGGC CACGGCCACA TCGATGCCGG AGTTCTTCAC CAACACTATC 
AGCACCCTGA TCCTGGAGTC GGACATGGGT CTGGGTACGA TCATCGGCTC GCTCATGTTC 
AACACCCTGG GCGTGGCGGG CCTGGCGGCG CTGTCCATTG ACAAGCCGGT TCAGCTGGAC 
TGGTGGCCAA TAGCGAGGGA CTGCTTCATC TACATATTCA ACACGATTAT CCTGCTGGTA 
CTGGCCTGGG GCGGCAGCAT TAGCTTCGTC GAGTCCTGCA TCATGATGGG CTTCCTAGTG 
CTCTACTATC TGATCACCTT CAACAACAAC AAGTTCATGC CAGCCATTCG CGTATTCATC 
GAGGATCGCA TGAACTGCTG CTTCTCCACG CGTTACGATC TAACGGAACC TCCGGAGAAC 
AGCGCCAAGG CACAGCTTCC GCTGAAGAAG GATCCCCTGA CGGGCGATGG CCTGTTCGTG 
GTAAACTTGC CAGAGAACAC CTCGTCGATG TCATCCACCG CCAATCTGAC TCACTCCAAG 
CATTGGAACG GCGATGACAG CGATGAAGAC GATATGCCAA AAAGCATCTA CGCCTATCCT 
CACGACGCCT CGCGCTTCAT GAAGAGCTGG TGGATCTTCG TTTTCCCGAT TAAGTTCACC 
CTCTCTTGGC TGATCCCTCA TCCGATGAAG CACCGCCGCC TCTACCCACT GTCCTTCATC 
ATGTGCATCC TCTGCATCGG GGGCAATGCC TACCTGATTG TCTGGATGCT GACGGCTTTC 
GGAGTGGCCA TCCATGTGCC AACAATAGTA ATGGGTCTCA CTTTCCTGGC TGCCGGCTCC 
ACCATGCCCG AGGCCGTCTC CAGTCTGATA TCCCTGAGGA ACGGTGAAAG CGGAATCGGA 
GTCTCGAACT CGCTGGGCGC CAACTCGCTG GCTATCCTGC TTTCTCTTGG CGTGCCCTGG 
TTCATCAAGA ACTGCCTAAA CTATGGAAGT GGGGAGCCGC AACAGGTCGG CACCCAGGGC 
ATCGAGTACA ATATCCTGAT ACTAATCATT TCAACGGTGG CCCTCTTCAT AATACTCAGC 
TTCAGTGGCT ACCGCTTGAC CAAGCGGGTG GGCATCGCTC TCTTCACCGT CTACGGAGTG 
TTTATCGTTC TTCAGATTCT TATTGAGATG AATGTGTTCT TCCCCAGGGA CTGCAGCAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA27534 ( XM_015185957.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015041443.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA27534), mRNA.

Price & Availability↑

CloneID	ODe24649
Clone ID Related Accession (Same CDS sequence)	XM_015185957.1
Accession Version	XM_015185957.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589960). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATTTCG TTTGCACAGC CAGCGCGACT CCAACCGGCA ATGAGAGCAA CTTTCTGGAG 
GCATCCCTGA GATACTTTGA TGAGCAGCTG CTCTACGACA TACTGCCACG TGCCTCGTGC 
CCGGGCGACG ATGAGACCTC ACCGATGGAT GAGTTTTCTG ACCTCTTTAC AGTGGAACAG 
CTTCGACAGG GCTGGGTGGT GCTTCATGTC TTTGCTGCGG TATACTTCTT TATACTGCTG 
GCCATAATAT GCAACGACTA CTTCCTGCCC ACGGTGGAGT GCATCTGCGA GGACCTGCAC 
CTGTCCAAGG ACGTGGCGGC GGCCACCTTT ATGGCCACGG CCACATCGAT GCCGGAGTTC 
TTCACCAACA CTATCAGCAC CCTGATCCTG GAGTCGGACA TGGGTCTGGG TACGATCATC 
GGCTCGCTCA TGTTCAACAC CCTGGGCGTG GCGGGCCTGG CGGCGCTGTC CATTGACAAG 
CCGGTTCAGC TGGACTGGTG GCCAATAGCG AGGGACTGCT TCATCTACAT ATTCAACACG 
ATTATCCTGC TGGTACTGGC CTGGGGCGGC AGCATTAGCT TCGTCGAGTC CTGCATCATG 
ATGGGCTTCC TAGTGCTCTA CTATCTGATC ACCTTCAACA ACAACAAGTT CATGCCAGCC 
ATTCGCGTAT TCATCGAGGA TCGCATGAAC TGCTGCTTCT CCACGCGTTA CGATCTAACG 
GAACCTCCGG AGAACAGCGC CAAGGCACAG CTTCCGCTGA AGAAGGATCC CCTGACGGGC 
GATGGCCTGT TCGTGGTAAA CTTGCCAGAG AACACCTCGT CGATGTCATC CACCGCCAAT 
CTGACTCACT CCAAGCATTG GAACGGCGAT GACAGCGATG AAGACGATAT GCCAAAAAGC 
ATCTACGCCT ATCCTCACGA CGCCTCGCGC TTCATGAAGA GCTGGTGGAT CTTCGTTTTC 
CCGATTAAGT TCACCCTCTC TTGGCTGATC CCTCATCCGA TGAAGCACCG CCGCCTCTAC 
CCACTGTCCT TCATCATGTG CATCCTCTGC ATCGGGGGCA ATGCCTACCT GATTGTCTGG 
ATGCTGACGG CTTTCGGAGT GGCCATCCAT GTGCCAACAA TAGTAATGGG TCTCACTTTC 
CTGGCTGCCG GCTCCACCAT GCCCGAGGCC GTCTCCAGTC TGATATCCCT GAGGAACGGT 
GAAAGCGGAA TCGGAGTCTC GAACTCGCTG GGCGCCAACT CGCTGGCTAT CCTGCTTTCT 
CTTGGCGTGC CCTGGTTCAT CAAGAACTGC CTAAACTATG GAAGTGGGGA GCCGCAACAG 
GTCGGCACCC AGGGCATCGA GTACAATATC CTGATACTAA TCATTTCAAC GGTGGCCCTC 
TTCATAATAC TCAGCTTCAG TGGCTACCGC TTGACCAAGC GGGTGGGCAT CGCTCTCTTC 
ACCGTCTACG GAGTGTTTAT CGTTCTTCAG ATTCTTATTG AGATGAATGT GTTCTTCCCC 
AGGGACTGCA GCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015041443.1
CDS	26..1543
Translation	MHFVCTASATPTGNESNFLEASLRYFDEQLLYDILPRASCPGDDETSPMDEFSDLFTVEQLRQGWVVLHVFAAVYFFILLAIICNDYFLPTVECICEDLHLSKDVAAATFMATATSMPEFFTNTISTLILESDMGLGTIIGSLMFNTLGVAGLAALSIDKPVQLDWWPIARDCFIYIFNTIILLVLAWGGSISFVESCIMMGFLVLYYLITFNNNKFMPAIRVFIEDRMNCCFSTRYDLTEPPENSAKAQLPLKKDPLTGDGLFVVNLPENTSSMSSTANLTHSKHWNGDDSDEDDMPKSIYAYPHDASRFMKSWWIFVFPIKFTLSWLIPHPMKHRRLYPLSFIMCILCIGGNAYLIVWMLTAFGVAIHVPTIVMGLTFLAAGSTMPEAVSSLISLRNGESGIGVSNSLGANSLAILLSLGVPWFIKNCLNYGSGEPQQVGTQGIEYNILILIISTVALFIILSFSGYRLTKRVGIALFTVYGVFIVLQILIEMNVFFPRDCSS

Target ORF information:

RefSeq Version	XM_015185957.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA27534), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015185957.1

ORF Insert Sequence:

ATGCATTTCG TTTGCACAGC CAGCGCGACT CCAACCGGCA ATGAGAGCAA CTTTCTGGAG 
GCATCCCTGA GATACTTTGA TGAGCAGCTG CTCTACGACA TACTGCCACG TGCCTCGTGC 
CCGGGCGACG ATGAGACCTC ACCGATGGAT GAGTTTTCTG ACCTCTTTAC AGTGGAACAG 
CTTCGACAGG GCTGGGTGGT GCTTCATGTC TTTGCTGCGG TATACTTCTT TATACTGCTG 
GCCATAATAT GCAACGACTA CTTCCTGCCC ACGGTGGAGT GCATCTGCGA GGACCTGCAC 
CTGTCCAAGG ACGTGGCGGC GGCCACCTTT ATGGCCACGG CCACATCGAT GCCGGAGTTC 
TTCACCAACA CTATCAGCAC CCTGATCCTG GAGTCGGACA TGGGTCTGGG TACGATCATC 
GGCTCGCTCA TGTTCAACAC CCTGGGCGTG GCGGGCCTGG CGGCGCTGTC CATTGACAAG 
CCGGTTCAGC TGGACTGGTG GCCAATAGCG AGGGACTGCT TCATCTACAT ATTCAACACG 
ATTATCCTGC TGGTACTGGC CTGGGGCGGC AGCATTAGCT TCGTCGAGTC CTGCATCATG 
ATGGGCTTCC TAGTGCTCTA CTATCTGATC ACCTTCAACA ACAACAAGTT CATGCCAGCC 
ATTCGCGTAT TCATCGAGGA TCGCATGAAC TGCTGCTTCT CCACGCGTTA CGATCTAACG 
GAACCTCCGG AGAACAGCGC CAAGGCACAG CTTCCGCTGA AGAAGGATCC CCTGACGGGC 
GATGGCCTGT TCGTGGTAAA CTTGCCAGAG AACACCTCGT CGATGTCATC CACCGCCAAT 
CTGACTCACT CCAAGCATTG GAACGGCGAT GACAGCGATG AAGACGATAT GCCAAAAAGC 
ATCTACGCCT ATCCTCACGA CGCCTCGCGC TTCATGAAGA GCTGGTGGAT CTTCGTTTTC 
CCGATTAAGT TCACCCTCTC TTGGCTGATC CCTCATCCGA TGAAGCACCG CCGCCTCTAC 
CCACTGTCCT TCATCATGTG CATCCTCTGC ATCGGGGGCA ATGCCTACCT GATTGTCTGG 
ATGCTGACGG CTTTCGGAGT GGCCATCCAT GTGCCAACAA TAGTAATGGG TCTCACTTTC 
CTGGCTGCCG GCTCCACCAT GCCCGAGGCC GTCTCCAGTC TGATATCCCT GAGGAACGGT 
GAAAGCGGAA TCGGAGTCTC GAACTCGCTG GGCGCCAACT CGCTGGCTAT CCTGCTTTCT 
CTTGGCGTGC CCTGGTTCAT CAAGAACTGC CTAAACTATG GAAGTGGGGA GCCGCAACAG 
GTCGGCACCC AGGGCATCGA GTACAATATC CTGATACTAA TCATTTCAAC GGTGGCCCTC 
TTCATAATAC TCAGCTTCAG TGGCTACCGC TTGACCAAGC GGGTGGGCAT CGCTCTCTTC 
ACCGTCTACG GAGTGTTTAT CGTTCTTCAG ATTCTTATTG AGATGAATGT GTTCTTCCCC 
AGGGACTGCA GCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA27534 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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