Dpse\GA23460 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA23460
Entrez Gene ID	6900677
Full Name	GA23460 gene product from transcript GA23460-RB
Synonyms	Dpse_GA19670,GA19670,GA23460,dpse_GLEANR_12289
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_002135005.2	XP_002135041.2	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA23460 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA23460 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe25842	XM_002135005.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA23460), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA23460 ( XM_002135005.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_002135041.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA23460), mRNA.

Price & Availability↑

CloneID	ODe25842
Clone ID Related Accession (Same CDS sequence)	XM_002135005.2
Accession Version	XM_002135005.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589955). On Dec 22, 2015 this sequence version replaced XM_002135005.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGGCG CAATTGCGCA TAACTTAGCG AATGGGCAAA ATCGCACCAG CGACGTGGAG 
GCCGGCCAGA TGAAACACTT CTCGGCCCTG CACCTGCGCC GCCAGGTTAT GAGGGGTTTG 
GCTGCTGAAA ACTTTAGGAC GCCAACGAAA ATTCAGGCCG CAGCCATACC CATTGCTCTG 
ACTGGAATGG ATTTGTTGGT GCAGTCCAAG AGCGGCACAG GCAAGACGCT GATCTATGTG 
GTGACCGCGC TGCAGATGTG CAGCCTTTCG ACTCAGCATC CAGAGGTTTT GGTAATTTTG 
CCCACACGGG AGCTGGCACT TCAGGTGCAC GACATATTCC GTTTTTTGGG TGAGAAACTG 
CGGTCCTTCA AGGTCAGTTC CTTTATGGGC GGCACCGATG TGACCAGGGA TCGAGAGAAA 
CTACGCAACT GCCATGTGGC CATTGGTACA CCGGGTCGCC TGCTGCAACT GCACGAAAAG 
GGCGTTCTTA ATATGTCCAT GGTGAAATTG CTGGTGCTCG ACGAGGCCGA TCAGCTCTAC 
GTGACGGCGA GCCTTCAGAA GACGGTGAAC GCCCTGATTG CGGTGCTGCC GCTGCAGCGC 
CAGGTCATTG CCTGCAGTGC CACCTTCGAT CAGAATCTGG ACGAGAAGAT AGCCAAAATG 
ATGGAAAAGC CCGTCCTGAT ATCGAATAGC GAGCGCGCCA CCGTACTCCT GGGCATCCGA 
CAGTTTGTCT ACGAGTTGCC CGAACAGGTC AACAACCTAC TCGAGATGCG TCTGAAGCTG 
GAGGCCCTGA AGAAGATCTT CGCCCAGCTG AACTACGAGC AGGCAGTGCT CTTTTCCAAC 
TCGAAAATGC GTGCGGACTC GTACTGCAGT TACCTAAACG CCGGCAAAAT ACCGTGCGCG 
CTACTCTCCG GCGACCTCGC TCAGGAGGAT CGCTCCAAAG TCTTCGAGGG CTATCGCAAC 
TTTTCGGTGC GCACCATAGT GGCCACGGAT CTGATCGCCC GAGGCGTGGA CTCGCATCAC 
GCCAACTTAG TGATCAATCT GGACCCGCCG ACTGATCACA TAACCTATCT GCACCGCATC 
GGGCGTGCCG GACGCTTCGG CTCCAAGGCC ATAGCCATCA CATTCATCTC GTCGGCGAAG 
CAAAGCGAGA ACTTTAAGAA GATTCTGGCC AAGGCCGGCA CCGGCATGTC CGTCCTGCAG 
TTCCCCACAG AAGGGCCGCC GGCAAAGGAC TTTAACTACT TTGAGTTTGA TGCCTATCAA 
TTTCCATATT ATTTTAAGAC CGAAGCCTGT GAGCAGGACA ATAACGAGCT GAATCGAATC 
ATAAAACCGA AGTTTTATGC ACAGCTGGCC ATCAAAATAG CCGAAGAAAA TGCTGAACAA 
CCCACGGATG GGACTCCTGT TGAGGAAAAG ACACAGGATC CTAGGGAGCA AATCAATGAG 
CCCAAAGTAG AAGAGACTGC TCCTCAGGCT AAAGAGGAGC TCGTCAAACC AGAAGTTCTC 
TCTCCCGCTC AAGTGGCGGA GGAAAAGCCG ACCAATCTAC AATTTGAGAT TCTCACATAT 
CCCCCCATGG ACGAGGCCAA CAACAATCCT CTGGAAGACA CCAGCCGAGC ATTTGAGGTG 
CTCTCGTTGC CGGAACCCGT TGAGGAGATC CCCGCTTTGA ATGAGGAAAA GCCCAGCACC 
AGCTTTGCAG CCAAAAACAA AGAGAAACAA AGGCGCACAT CCGAAACGCA AAAGGCTGCG 
AAAGCCCCCG TAGAGCCGCA ACCATGCTTC GCAGACAACA AGGAGAATCG ACCATTGAAT 
GGAGTAGAAA CCGCCACAGA AAGCCGGTCA GGGTCCAACC AGACAGTGAT GGCAGTGGAT 
GGCTCTCCTC AAACATCAAA AACCATATCG GATGGCACAG CAAAGCGTCC AGAGTCCCAG 
CAGATGGCCA TGGGAGAGGA CTCAACGCAG ACATCACAGA CACCAACGGA GGCGTCACAA 
GAACCCACTC AGTCCACGGG CTGTTCGGTT GAAACACACC CGGGACCGCC AGTGAATTCG 
ATCAATACGA AGACCTACTG CCTGGTGGTG CCTGCCACGG AGAACAGCTC GACCACACTG 
CAGCCGCATG TCCTGTCGAA CACGGTGGAC GATGCCAGCA GCATTGTCTC GGACAGCCTG 
GAGTGCGGCT ATACGAGTGA TGCCTCATAT GTCAGCCACT ACACGGAGAG CGATGGCCAG 
ATTATTTGGC AGCGCTTCAG GGCCCGTCAG CGACTGCTCA AGAGGCGCCG TCGATCCCCC 
AAGGCTCATC GCTGGCATCA AAAATTCTCA ACATTTTTAC GAGTGCAGCC AGCATTCAGG 
AGTGGCAGGA AACCCGGCAT CGATTCTCTG CTGCCAGTTC TGGCCCACCA TCAGTTACTG 
ACGAAGTTTG AGAACAGGGA GAGCATAATC AAACGCTTGA ATGCCTACAA AACGATATTC 
AAGGTCAAGA ATGAGTCGCG CTGGCTGGAC GCACTCTACA GGACGGCAAT GGAAGTTTAC 
TATACGAATT ATGGCCCTGC CGCAGAGCAG CTGCCGCCGC CAGGTCTGGC AAAGCTGAGC 
GTTCATGTTG CCCATCAGAT GGACATTCCA GCCGTGGCTG CTCCGCCGGC AGCTGTGGTT 
GTCGTTGAAG TCGAAAACGA GGCAGACGAT GAGGCGGACT CATCCGAATC GGCCTTGTCA 
TCAGGTGACG ATGAGATTTT TGAAGAAACG AGCTCGCCAT CGAGCGGCTT TGGGGAGAGT 
ACAGAGGAGA GTGCCTCTTC GGGCATTGAG ACGTCTGTCT ACGATCCTTC TGGTTCCTCA 
AATGGCCACG ACAATGATGA TGATGAGGAA TACTATGACA ATGAGGAAGA CTATGACGAT 
GAAGAGGACG AGGAGGAGGA GGAAGAGGAG GAGGAGGATG ATGCATTTAG CAATGCTGGG 
AGCACTGACA ATAGCTTTGT GATGAGAAAC GTACGCCATC GACCTACTAA ACGGGCAGCA 
AGTGACAGAA CTGCCAGCAG TTCTAGCAAC GAAAGCAATG CTGGTAGAGG CCACATTTCT 
CCAGATCGAA CCAATGCCAA CGGTGCCTGC AGTGCTCCAA AGACTGATGA AAGCAGTGCC 
GCAGAGACTG ATGAAAGCAG TGCCGCAGAG ACTGATGAAA GCAGTGACCC CGAGGATCGA 
GAGTCAGTAC AACCGGATGT TGCCCAAATA AACCGGGCCA AAAAACTCTG GGAGGAGACC 
TTCAACAGGC AGTATCTGAT AATTGCCAAC CACGTGGCCA ATCACATGGC CCAGTTCCAG 
GACGAAGAGG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002135041.2
CDS	97..3411
Translation	MEGAIAHNLANGQNRTSDVEAGQMKHFSALHLRRQVMRGLAAENFRTPTKIQAAAIPIALTGMDLLVQSKSGTGKTLIYVVTALQMCSLSTQHPEVLVILPTRELALQVHDIFRFLGEKLRSFKVSSFMGGTDVTRDREKLRNCHVAIGTPGRLLQLHEKGVLNMSMVKLLVLDEADQLYVTASLQKTVNALIAVLPLQRQVIACSATFDQNLDEKIAKMMEKPVLISNSERATVLLGIRQFVYELPEQVNNLLEMRLKLEALKKIFAQLNYEQAVLFSNSKMRADSYCSYLNAGKIPCALLSGDLAQEDRSKVFEGYRNFSVRTIVATDLIARGVDSHHANLVINLDPPTDHITYLHRIGRAGRFGSKAIAITFISSAKQSENFKKILAKAGTGMSVLQFPTEGPPAKDFNYFEFDAYQFPYYFKTEACEQDNNELNRIIKPKFYAQLAIKIAEENAEQPTDGTPVEEKTQDPREQINEPKVEETAPQAKEELVKPEVLSPAQVAEEKPTNLQFEILTYPPMDEANNNPLEDTSRAFEVLSLPEPVEEIPALNEEKPSTSFAAKNKEKQRRTSETQKAAKAPVEPQPCFADNKENRPLNGVETATESRSGSNQTVMAVDGSPQTSKTISDGTAKRPESQQMAMGEDSTQTSQTPTEASQEPTQSTGCSVETHPGPPVNSINTKTYCLVVPATENSSTTLQPHVLSNTVDDASSIVSDSLECGYTSDASYVSHYTESDGQIIWQRFRARQRLLKRRRRSPKAHRWHQKFSTFLRVQPAFRSGRKPGIDSLLPVLAHHQLLTKFENRESIIKRLNAYKTIFKVKNESRWLDALYRTAMEVYYTNYGPAAEQLPPPGLAKLSVHVAHQMDIPAVAAPPAAVVVVEVENEADDEADSSESALSSGDDEIFEETSSPSSGFGESTEESASSGIETSVYDPSGSSNGHDNDDDEEYYDNEEDYDDEEDEEEEEEEEEDDAFSNAGSTDNSFVMRNVRHRPTKRAASDRTASSSSNESNAGRGHISPDRTNANGACSAPKTDESSAAETDESSAAETDESSDPEDRESVQPDVAQINRAKKLWEETFNRQYLIIANHVANHMAQFQDEED

Target ORF information:

RefSeq Version	XM_002135005.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA23460), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002135005.2

ORF Insert Sequence:

ATGGAGGGCG CAATTGCGCA TAACTTAGCG AATGGGCAAA ATCGCACCAG CGACGTGGAG 
GCCGGCCAGA TGAAACACTT CTCGGCCCTG CACCTGCGCC GCCAGGTTAT GAGGGGTTTG 
GCTGCTGAAA ACTTTAGGAC GCCAACGAAA ATTCAGGCCG CAGCCATACC CATTGCTCTG 
ACTGGAATGG ATTTGTTGGT GCAGTCCAAG AGCGGCACAG GCAAGACGCT GATCTATGTG 
GTGACCGCGC TGCAGATGTG CAGCCTTTCG ACTCAGCATC CAGAGGTTTT GGTAATTTTG 
CCCACACGGG AGCTGGCACT TCAGGTGCAC GACATATTCC GTTTTTTGGG TGAGAAACTG 
CGGTCCTTCA AGGTCAGTTC CTTTATGGGC GGCACCGATG TGACCAGGGA TCGAGAGAAA 
CTACGCAACT GCCATGTGGC CATTGGTACA CCGGGTCGCC TGCTGCAACT GCACGAAAAG 
GGCGTTCTTA ATATGTCCAT GGTGAAATTG CTGGTGCTCG ACGAGGCCGA TCAGCTCTAC 
GTGACGGCGA GCCTTCAGAA GACGGTGAAC GCCCTGATTG CGGTGCTGCC GCTGCAGCGC 
CAGGTCATTG CCTGCAGTGC CACCTTCGAT CAGAATCTGG ACGAGAAGAT AGCCAAAATG 
ATGGAAAAGC CCGTCCTGAT ATCGAATAGC GAGCGCGCCA CCGTACTCCT GGGCATCCGA 
CAGTTTGTCT ACGAGTTGCC CGAACAGGTC AACAACCTAC TCGAGATGCG TCTGAAGCTG 
GAGGCCCTGA AGAAGATCTT CGCCCAGCTG AACTACGAGC AGGCAGTGCT CTTTTCCAAC 
TCGAAAATGC GTGCGGACTC GTACTGCAGT TACCTAAACG CCGGCAAAAT ACCGTGCGCG 
CTACTCTCCG GCGACCTCGC TCAGGAGGAT CGCTCCAAAG TCTTCGAGGG CTATCGCAAC 
TTTTCGGTGC GCACCATAGT GGCCACGGAT CTGATCGCCC GAGGCGTGGA CTCGCATCAC 
GCCAACTTAG TGATCAATCT GGACCCGCCG ACTGATCACA TAACCTATCT GCACCGCATC 
GGGCGTGCCG GACGCTTCGG CTCCAAGGCC ATAGCCATCA CATTCATCTC GTCGGCGAAG 
CAAAGCGAGA ACTTTAAGAA GATTCTGGCC AAGGCCGGCA CCGGCATGTC CGTCCTGCAG 
TTCCCCACAG AAGGGCCGCC GGCAAAGGAC TTTAACTACT TTGAGTTTGA TGCCTATCAA 
TTTCCATATT ATTTTAAGAC CGAAGCCTGT GAGCAGGACA ATAACGAGCT GAATCGAATC 
ATAAAACCGA AGTTTTATGC ACAGCTGGCC ATCAAAATAG CCGAAGAAAA TGCTGAACAA 
CCCACGGATG GGACTCCTGT TGAGGAAAAG ACACAGGATC CTAGGGAGCA AATCAATGAG 
CCCAAAGTAG AAGAGACTGC TCCTCAGGCT AAAGAGGAGC TCGTCAAACC AGAAGTTCTC 
TCTCCCGCTC AAGTGGCGGA GGAAAAGCCG ACCAATCTAC AATTTGAGAT TCTCACATAT 
CCCCCCATGG ACGAGGCCAA CAACAATCCT CTGGAAGACA CCAGCCGAGC ATTTGAGGTG 
CTCTCGTTGC CGGAACCCGT TGAGGAGATC CCCGCTTTGA ATGAGGAAAA GCCCAGCACC 
AGCTTTGCAG CCAAAAACAA AGAGAAACAA AGGCGCACAT CCGAAACGCA AAAGGCTGCG 
AAAGCCCCCG TAGAGCCGCA ACCATGCTTC GCAGACAACA AGGAGAATCG ACCATTGAAT 
GGAGTAGAAA CCGCCACAGA AAGCCGGTCA GGGTCCAACC AGACAGTGAT GGCAGTGGAT 
GGCTCTCCTC AAACATCAAA AACCATATCG GATGGCACAG CAAAGCGTCC AGAGTCCCAG 
CAGATGGCCA TGGGAGAGGA CTCAACGCAG ACATCACAGA CACCAACGGA GGCGTCACAA 
GAACCCACTC AGTCCACGGG CTGTTCGGTT GAAACACACC CGGGACCGCC AGTGAATTCG 
ATCAATACGA AGACCTACTG CCTGGTGGTG CCTGCCACGG AGAACAGCTC GACCACACTG 
CAGCCGCATG TCCTGTCGAA CACGGTGGAC GATGCCAGCA GCATTGTCTC GGACAGCCTG 
GAGTGCGGCT ATACGAGTGA TGCCTCATAT GTCAGCCACT ACACGGAGAG CGATGGCCAG 
ATTATTTGGC AGCGCTTCAG GGCCCGTCAG CGACTGCTCA AGAGGCGCCG TCGATCCCCC 
AAGGCTCATC GCTGGCATCA AAAATTCTCA ACATTTTTAC GAGTGCAGCC AGCATTCAGG 
AGTGGCAGGA AACCCGGCAT CGATTCTCTG CTGCCAGTTC TGGCCCACCA TCAGTTACTG 
ACGAAGTTTG AGAACAGGGA GAGCATAATC AAACGCTTGA ATGCCTACAA AACGATATTC 
AAGGTCAAGA ATGAGTCGCG CTGGCTGGAC GCACTCTACA GGACGGCAAT GGAAGTTTAC 
TATACGAATT ATGGCCCTGC CGCAGAGCAG CTGCCGCCGC CAGGTCTGGC AAAGCTGAGC 
GTTCATGTTG CCCATCAGAT GGACATTCCA GCCGTGGCTG CTCCGCCGGC AGCTGTGGTT 
GTCGTTGAAG TCGAAAACGA GGCAGACGAT GAGGCGGACT CATCCGAATC GGCCTTGTCA 
TCAGGTGACG ATGAGATTTT TGAAGAAACG AGCTCGCCAT CGAGCGGCTT TGGGGAGAGT 
ACAGAGGAGA GTGCCTCTTC GGGCATTGAG ACGTCTGTCT ACGATCCTTC TGGTTCCTCA 
AATGGCCACG ACAATGATGA TGATGAGGAA TACTATGACA ATGAGGAAGA CTATGACGAT 
GAAGAGGACG AGGAGGAGGA GGAAGAGGAG GAGGAGGATG ATGCATTTAG CAATGCTGGG 
AGCACTGACA ATAGCTTTGT GATGAGAAAC GTACGCCATC GACCTACTAA ACGGGCAGCA 
AGTGACAGAA CTGCCAGCAG TTCTAGCAAC GAAAGCAATG CTGGTAGAGG CCACATTTCT 
CCAGATCGAA CCAATGCCAA CGGTGCCTGC AGTGCTCCAA AGACTGATGA AAGCAGTGCC 
GCAGAGACTG ATGAAAGCAG TGCCGCAGAG ACTGATGAAA GCAGTGACCC CGAGGATCGA 
GAGTCAGTAC AACCGGATGT TGCCCAAATA AACCGGGCCA AAAAACTCTG GGAGGAGACC 
TTCAACAGGC AGTATCTGAT AATTGCCAAC CACGTGGCCA ATCACATGGC CCAGTTCCAG 
GACGAAGAGG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA23460 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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