Dpse\GA13122 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA13122
Entrez Gene ID	4816009
Full Name	GA13122 gene product from transcript GA13122-RB
Synonyms	GA13122,dpse_GLEANR_11415
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001355420.3	XP_001355456.3	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA13122 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA13122 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe23786	XM_001355420.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA13122), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA13122 ( XM_001355420.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001355456.3 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA13122), mRNA.

Price & Availability↑

CloneID	ODe23786
Clone ID Related Accession (Same CDS sequence)	XM_001355420.3
Accession Version	XM_001355420.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589961). On Dec 22, 2015 this sequence version replaced XM_001355420.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGCCA AAAACCAAAT GGGCGTCTTC ATGGAGAAGA TCAACACGAC GCTACAGTCG 
TGTCCTTCGG TTGTGTGCAT AAATGAGCCG GACACGCACA GTGCCAACAG CACAGAGATA 
CTGCAGCAGC CAACGCCAGA GAGTACTCTA GAGAAGAAGC AGGTGCAGGG AGGAGCAGCA 
GCTAGCCTAA GTGGCAACTG TATTACAATT AATGCGGCAA CAGCAGCTGG AGGCACCTCT 
GCGGGCGTGA CAAAGATAAC GATAACAGGC GACATCGGCA ACAACAGCAG CAGCAACAAC 
AGCTCATACG AGAGTCAGCC TCCCACCATT CTGAAGACGA ACCACAACAG CAGTTTGGTG 
TCCATCACGA GCTTAAACAG CTGCGGCGAC CTCAGCTACA GTCGGCAGGG CGTCAGCCCA 
GAGGGGGGTA GCACAGCCAC ACTGCCCCTG GGAGGAGGCG GCAGCAACAG CAGCCACAGC 
AGCCAAAGCA GCCCGCACGG CAGCAGCGAC ACCTCATCGG GAAATTTCCA AGAACGCTTC 
GACTTTGAGC ACTGGAAGGG ATTACTCAGC GACTACAACT GTTTCCACAG TCTCTATCGG 
TATTGGAATC AGTGCAGCAG CAGCCACAGC AGCAGTGGCA CTGGCAGTGG CAGTGCCACT 
AAAAGGCAAT CGTGCCACCC ACTGGATGCT CAGTCACGCA ACGATCATGC GCACGAAGGG 
AACAACAACG AAGCAGGATT GGGCAGCGGT CTGGGCAGTG GTTCATTCTA CACGAATAAT 
ATACTAGGAT ACACCTTTGG ATCCTTACCC TTCGGCTTCA AGCAGCAGCA CTCGCAGCAC 
TCGCAGCACT CGCAGCAGCA GCAGCAGCAG GACCTCGACA GCCAGAGCGG CAGCAGCAGC 
ACTCTAGGAT ACAAAAAGTC GTCTTCCTTT TACGGCAGCG CCAACGGCAA CGAAACGCCA 
CTCCAGAAAC ACTACAGACA ACAGCAGAAG CGAAAGATGC CCGTCTTCAG GGGCAGGAGA 
CCCTGGTGCG GGTGCTTCAA GGACGATGAA CCCCCCGAAA TCTGTGTGGT GGAGGGGGCC 
TTCACCCTGC AGACCCTCAC GCCCACACAG CCAATGCCGT CGGTGGATGA GCTGGACACC 
AAGTTCGCGG AACTGGTCGA AGAGCTCGAT CTGACAGCGC CCAACAAGGA GGCGATGCTC 
AGTCTGCCAG CCCAGAAGAA ATGGCAAATC TACTGCTCCC GCAAGCAGCC GCTGGACGCG 
ACAGATGGCC CCGATGGGCC CTCGGCGCTC GCCGGGCATC AGCCCCCGAC GGCCGAGCAC 
TATATCGAGC GGCTGAAGGA GCTGGTGGTG CACGTCTCCC TCTCGCCGGA GGACTCGCCC 
AGCCATGAGG GGGCCAGCAA TCGGCTGGAC AACCACGCGG CCCTCGTGGA CGCCCTGAAG 
ACGGCGCTGC GCACCTCCAC GCACAGCTTT GTGCTCCGCT TCGTGGAGCT GGACGGACTG 
CCGGCGCTGC TCAATCTCCT GCTGCAGCTG GACATACGTG TGGCCAACAG CTCGTTGCAC 
ACGAGTCTCA TTGGCTGCAT CAAGGCGCTG ATGAACAACT CGATGGGACG CGCCCATGTG 
CTGGCCCACC CCACGGCCAT AGACACGATT GCCCGCTCCC TGGCGGCGGA CAACATACGC 
ACGAAGATAG CGGCCCTGGA GATCCTGGGA GCCGTGTGCC TGGTGCCGGG AGGACATCGC 
AAGGTGCTGC AGGCGATGCT GCACTTCCAG GAGTTTGCCA CAGAGCGCAC GCGCTTCCAG 
AGCATTGTCA ATGACCTGGA CCGTTCGACC TATGCGTATA TGGACAATGT CAATCTGAAG 
ACCGCTCTGA TGTCTTTTGT GAATGCTGTC CTGAACTACG GCCCCGGGCA GGAGAATCTC 
GAGTTCAGGC TCCATTTGCG CTACGAATTT CTGATGCTGG GCATCCAGCC GGTGATCGAC 
AAGCTGCGGA CGCACGAGAA TGAGACGCTG GACAGGCATT TGGACTTCTT TGAGATGGTG 
AGGGCGGAGG ATGAGAAGGA GTTTGCCCGT CGCTTCAACG AGGAGCATGT GGACACGAAG 
AGCGCTGGGT CCATGTTTGA GCTGCTGCGC CGCAAGCTCA GCCATTCACC AGCGTATCCC 
CACATGCTGT CGCTGCTGCA GCATATGCTG TTGCTACCCT ATACGGGCCA CTGCACTGAA 
CATTGGCTGC TGATTGATCG CGTGGTCCAG CAGATTGTGC TGCAAGTGGA GCAGCGACCC 
AGCAGCGACC TTATACCCGA TCCCTTGGAG GATCCTGGCA AGCAGCTGAA GCTGGCCAGC 
GAGTCCCCTG TTCATGACCC GGATGTGGCA CCCCTGCAGA TCGATGTGGG CAAGCTGGTG 
CGTCTCCTGG TGAAGGAAGA GCAGCTCACG CAGGCGCGCA AGCGGGCGGA TGAATTGGAG 
CGCGAGAACT TTGAAGTACA GTCGCGTCTG GCCAAGAAGG AACAGGAACT CGATCTGCGA 
ATGCAGGAGA AGGAGGACCT GGAGACGGGC CTGGCACGCA TGCGCGAACG CCTCGAGAAG 
GAGTCCGCGC AGCACTCGCA GGCCGTGCAG CGGGCCCAGA GTGCCGAAAT GAAGGCCGAG 
GATCTGCAAC ATCGCCTGCA CAGCGAGCAG CAGGAGCGGG CGCGTCTCGA GCGGCTCGTG 
ACCGAAGGCA GCATACCCGA TGACCAAAAG GTGGCTGGCC TGACAGGTTG CAACGGTGCT 
GTTTCGCCGC CACCACCGCC ACCGCCGATG CTGAATGCCA TTCCACCGCC GCCGGCACCG 
CCAATGGCGC CAGCAATGCT GCCGCCCCCA CCGCCGCCAT GTCCGGGCGC CCCACCACCG 
CCGCCCAGCA TGACGCCAGC GATGACTCCT GTGGCCCCCA AGGTGGAGCT GCCCAAAAAG 
AATGTGCCAC AGCCCACAAA TCCCTTGAAG AGCTTCAACT GGTCCAAGCT GCCGGATGCC 
AAGCTTCAGG GCACAGTCTG GAGCGAACTG GACGAGAGCA AGCTGTACAA CAACATGGAG 
CTGGAGTCGA TTGACAAGCT CTTTTCGGCC TACCAGAAGA ATGGAGTGCC AGCACATGAT 
GGCTCATACG AGGATCTGCG ACCCACGGGA CAGAAGAACA AACAAAAGGT TCTCTCAGTG 
ATCGATGGAC GCCGAGCCCA AAACTGCACG ATTCTGTTGA GCAAACTGAA GATGAGCGAT 
GTGGAGATAT CCAAGGCCAT CCTCTCCATG GACTGCAATG AGCAGCTGCA ATTGGACATG 
GTCGAGCAGC TGTTGAAATT CACGCCCTCT GCCGAAGAGC GGGCTTTGCT GGACGAGCAC 
AGCGAGGACA TTGAATCGCT GGCACGAGCC GATCGTTTTC TCTATGAGAT ATCCAAGATA 
CCGCACTACG AGCAGCGGCT GAAGAGTCTG CACTACAAGA AGCGCTTTAT GCTGACCGTC 
AACGATCTGA CGCCGCGCAT CAAGAGCGTG ATGGAGGCCT CACGCGAGGT GGCACGCTCT 
CGGCGTCTGC GCAAGTTATT GGAGCTAGTC CTGGCATTGG GCAACTACAT GAATCGGGGG 
GCACGCGGTA ATGCCTCTGG CTTCCGTTTG GCGTCGCTCA ATCGTCTGGC CGATACCAAA 
TCGAGTGCCG CCAAGGGCAC AACGCTGCTC CATTATCTCG TGCAGGTTAT CGAAAAGAAG 
TTCAAGGACC TGCTGAAGCT GGAGGATGAT ATACCGCATG TGCGCGGTGC CTCGAAGGTG 
TCGCTGGGCG AGATGGACAA GGACATACAG ATGCTGCGCA CCGGGCTGGC GGATGTGGCC 
CGTGAGATTG AGTTCCATCG GAGTTCGGGT CCCGCACAGC AGGGTGATCG CTTTCTGCCC 
GTGATGCGGG AGTTCCATGC CCAGGCATCG GTGCGCTTTG CCGAGCTGGA GGACAAGTTC 
CAGGACATGA AGACGCGCTT CGATCGTGCC GTCCGTCTGT TCGGTGAAGA TGGATCCGTG 
TTGCAGCCGG ACGAGTTTTT TGGCATCTTT GATTCGTTCT TGGGAGCCTT TGCCGAGGCG 
CGTCACGACA ACGAAAGCTT CCGCCGACGG CAGGAGGAGG AGGAGAAGCG GGCCAAGCAG 
GAGGCCGAGC TCAAGAAGCG GACCATCGAT CGCAAGAACA AGACCGGCCT CATGAGCAGT 
GTTGCACGCA ACCTCGGCCT AAAGTCCTCA TCCCCCACGA ATGGAGGCGA CTCCCCGGGC 
AAGGGTGGCG GCGACAACAA GGGCGAGTTC GATGACCTTA TCTCGGCCCT GCGCACGGGC 
GATGTCTTTG GCGAGGACAT GGCCAAGTTT AAGCGATCGC GCAAGGCGCG TGTACTAAAT 
GGTGGATCAG GAGCGGGAGG TGCCACCGGC AACACCTCAC CGCCAAGGCA TGGCAGCCTT 
CAGCGCGAGG AGAGCGGACG CGAACGGGAG CGCACTGTGA GGCGTCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001355456.3
CDS	313..4803
Translation	MSAKNQMGVFMEKINTTLQSCPSVVCINEPDTHSANSTEILQQPTPESTLEKKQVQGGAAASLSGNCITINAATAAGGTSAGVTKITITGDIGNNSSSNNSSYESQPPTILKTNHNSSLVSITSLNSCGDLSYSRQGVSPEGGSTATLPLGGGGSNSSHSSQSSPHGSSDTSSGNFQERFDFEHWKGLLSDYNCFHSLYRYWNQCSSSHSSSGTGSGSATKRQSCHPLDAQSRNDHAHEGNNNEAGLGSGLGSGSFYTNNILGYTFGSLPFGFKQQHSQHSQHSQQQQQQDLDSQSGSSSTLGYKKSSSFYGSANGNETPLQKHYRQQQKRKMPVFRGRRPWCGCFKDDEPPEICVVEGAFTLQTLTPTQPMPSVDELDTKFAELVEELDLTAPNKEAMLSLPAQKKWQIYCSRKQPLDATDGPDGPSALAGHQPPTAEHYIERLKELVVHVSLSPEDSPSHEGASNRLDNHAALVDALKTALRTSTHSFVLRFVELDGLPALLNLLLQLDIRVANSSLHTSLIGCIKALMNNSMGRAHVLAHPTAIDTIARSLAADNIRTKIAALEILGAVCLVPGGHRKVLQAMLHFQEFATERTRFQSIVNDLDRSTYAYMDNVNLKTALMSFVNAVLNYGPGQENLEFRLHLRYEFLMLGIQPVIDKLRTHENETLDRHLDFFEMVRAEDEKEFARRFNEEHVDTKSAGSMFELLRRKLSHSPAYPHMLSLLQHMLLLPYTGHCTEHWLLIDRVVQQIVLQVEQRPSSDLIPDPLEDPGKQLKLASESPVHDPDVAPLQIDVGKLVRLLVKEEQLTQARKRADELERENFEVQSRLAKKEQELDLRMQEKEDLETGLARMRERLEKESAQHSQAVQRAQSAEMKAEDLQHRLHSEQQERARLERLVTEGSIPDDQKVAGLTGCNGAVSPPPPPPPMLNAIPPPPAPPMAPAMLPPPPPPCPGAPPPPPSMTPAMTPVAPKVELPKKNVPQPTNPLKSFNWSKLPDAKLQGTVWSELDESKLYNNMELESIDKLFSAYQKNGVPAHDGSYEDLRPTGQKNKQKVLSVIDGRRAQNCTILLSKLKMSDVEISKAILSMDCNEQLQLDMVEQLLKFTPSAEERALLDEHSEDIESLARADRFLYEISKIPHYEQRLKSLHYKKRFMLTVNDLTPRIKSVMEASREVARSRRLRKLLELVLALGNYMNRGARGNASGFRLASLNRLADTKSSAAKGTTLLHYLVQVIEKKFKDLLKLEDDIPHVRGASKVSLGEMDKDIQMLRTGLADVAREIEFHRSSGPAQQGDRFLPVMREFHAQASVRFAELEDKFQDMKTRFDRAVRLFGEDGSVLQPDEFFGIFDSFLGAFAEARHDNESFRRRQEEEEKRAKQEAELKKRTIDRKNKTGLMSSVARNLGLKSSSPTNGGDSPGKGGGDNKGEFDDLISALRTGDVFGEDMAKFKRSRKARVLNGGSGAGGATGNTSPPRHGSLQREESGRERERTVRRQ

Target ORF information:

RefSeq Version	XM_001355420.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA13122), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001355420.3

ORF Insert Sequence:

ATGTCTGCCA AAAACCAAAT GGGCGTCTTC ATGGAGAAGA TCAACACGAC GCTACAGTCG 
TGTCCTTCGG TTGTGTGCAT AAATGAGCCG GACACGCACA GTGCCAACAG CACAGAGATA 
CTGCAGCAGC CAACGCCAGA GAGTACTCTA GAGAAGAAGC AGGTGCAGGG AGGAGCAGCA 
GCTAGCCTAA GTGGCAACTG TATTACAATT AATGCGGCAA CAGCAGCTGG AGGCACCTCT 
GCGGGCGTGA CAAAGATAAC GATAACAGGC GACATCGGCA ACAACAGCAG CAGCAACAAC 
AGCTCATACG AGAGTCAGCC TCCCACCATT CTGAAGACGA ACCACAACAG CAGTTTGGTG 
TCCATCACGA GCTTAAACAG CTGCGGCGAC CTCAGCTACA GTCGGCAGGG CGTCAGCCCA 
GAGGGGGGTA GCACAGCCAC ACTGCCCCTG GGAGGAGGCG GCAGCAACAG CAGCCACAGC 
AGCCAAAGCA GCCCGCACGG CAGCAGCGAC ACCTCATCGG GAAATTTCCA AGAACGCTTC 
GACTTTGAGC ACTGGAAGGG ATTACTCAGC GACTACAACT GTTTCCACAG TCTCTATCGG 
TATTGGAATC AGTGCAGCAG CAGCCACAGC AGCAGTGGCA CTGGCAGTGG CAGTGCCACT 
AAAAGGCAAT CGTGCCACCC ACTGGATGCT CAGTCACGCA ACGATCATGC GCACGAAGGG 
AACAACAACG AAGCAGGATT GGGCAGCGGT CTGGGCAGTG GTTCATTCTA CACGAATAAT 
ATACTAGGAT ACACCTTTGG ATCCTTACCC TTCGGCTTCA AGCAGCAGCA CTCGCAGCAC 
TCGCAGCACT CGCAGCAGCA GCAGCAGCAG GACCTCGACA GCCAGAGCGG CAGCAGCAGC 
ACTCTAGGAT ACAAAAAGTC GTCTTCCTTT TACGGCAGCG CCAACGGCAA CGAAACGCCA 
CTCCAGAAAC ACTACAGACA ACAGCAGAAG CGAAAGATGC CCGTCTTCAG GGGCAGGAGA 
CCCTGGTGCG GGTGCTTCAA GGACGATGAA CCCCCCGAAA TCTGTGTGGT GGAGGGGGCC 
TTCACCCTGC AGACCCTCAC GCCCACACAG CCAATGCCGT CGGTGGATGA GCTGGACACC 
AAGTTCGCGG AACTGGTCGA AGAGCTCGAT CTGACAGCGC CCAACAAGGA GGCGATGCTC 
AGTCTGCCAG CCCAGAAGAA ATGGCAAATC TACTGCTCCC GCAAGCAGCC GCTGGACGCG 
ACAGATGGCC CCGATGGGCC CTCGGCGCTC GCCGGGCATC AGCCCCCGAC GGCCGAGCAC 
TATATCGAGC GGCTGAAGGA GCTGGTGGTG CACGTCTCCC TCTCGCCGGA GGACTCGCCC 
AGCCATGAGG GGGCCAGCAA TCGGCTGGAC AACCACGCGG CCCTCGTGGA CGCCCTGAAG 
ACGGCGCTGC GCACCTCCAC GCACAGCTTT GTGCTCCGCT TCGTGGAGCT GGACGGACTG 
CCGGCGCTGC TCAATCTCCT GCTGCAGCTG GACATACGTG TGGCCAACAG CTCGTTGCAC 
ACGAGTCTCA TTGGCTGCAT CAAGGCGCTG ATGAACAACT CGATGGGACG CGCCCATGTG 
CTGGCCCACC CCACGGCCAT AGACACGATT GCCCGCTCCC TGGCGGCGGA CAACATACGC 
ACGAAGATAG CGGCCCTGGA GATCCTGGGA GCCGTGTGCC TGGTGCCGGG AGGACATCGC 
AAGGTGCTGC AGGCGATGCT GCACTTCCAG GAGTTTGCCA CAGAGCGCAC GCGCTTCCAG 
AGCATTGTCA ATGACCTGGA CCGTTCGACC TATGCGTATA TGGACAATGT CAATCTGAAG 
ACCGCTCTGA TGTCTTTTGT GAATGCTGTC CTGAACTACG GCCCCGGGCA GGAGAATCTC 
GAGTTCAGGC TCCATTTGCG CTACGAATTT CTGATGCTGG GCATCCAGCC GGTGATCGAC 
AAGCTGCGGA CGCACGAGAA TGAGACGCTG GACAGGCATT TGGACTTCTT TGAGATGGTG 
AGGGCGGAGG ATGAGAAGGA GTTTGCCCGT CGCTTCAACG AGGAGCATGT GGACACGAAG 
AGCGCTGGGT CCATGTTTGA GCTGCTGCGC CGCAAGCTCA GCCATTCACC AGCGTATCCC 
CACATGCTGT CGCTGCTGCA GCATATGCTG TTGCTACCCT ATACGGGCCA CTGCACTGAA 
CATTGGCTGC TGATTGATCG CGTGGTCCAG CAGATTGTGC TGCAAGTGGA GCAGCGACCC 
AGCAGCGACC TTATACCCGA TCCCTTGGAG GATCCTGGCA AGCAGCTGAA GCTGGCCAGC 
GAGTCCCCTG TTCATGACCC GGATGTGGCA CCCCTGCAGA TCGATGTGGG CAAGCTGGTG 
CGTCTCCTGG TGAAGGAAGA GCAGCTCACG CAGGCGCGCA AGCGGGCGGA TGAATTGGAG 
CGCGAGAACT TTGAAGTACA GTCGCGTCTG GCCAAGAAGG AACAGGAACT CGATCTGCGA 
ATGCAGGAGA AGGAGGACCT GGAGACGGGC CTGGCACGCA TGCGCGAACG CCTCGAGAAG 
GAGTCCGCGC AGCACTCGCA GGCCGTGCAG CGGGCCCAGA GTGCCGAAAT GAAGGCCGAG 
GATCTGCAAC ATCGCCTGCA CAGCGAGCAG CAGGAGCGGG CGCGTCTCGA GCGGCTCGTG 
ACCGAAGGCA GCATACCCGA TGACCAAAAG GTGGCTGGCC TGACAGGTTG CAACGGTGCT 
GTTTCGCCGC CACCACCGCC ACCGCCGATG CTGAATGCCA TTCCACCGCC GCCGGCACCG 
CCAATGGCGC CAGCAATGCT GCCGCCCCCA CCGCCGCCAT GTCCGGGCGC CCCACCACCG 
CCGCCCAGCA TGACGCCAGC GATGACTCCT GTGGCCCCCA AGGTGGAGCT GCCCAAAAAG 
AATGTGCCAC AGCCCACAAA TCCCTTGAAG AGCTTCAACT GGTCCAAGCT GCCGGATGCC 
AAGCTTCAGG GCACAGTCTG GAGCGAACTG GACGAGAGCA AGCTGTACAA CAACATGGAG 
CTGGAGTCGA TTGACAAGCT CTTTTCGGCC TACCAGAAGA ATGGAGTGCC AGCACATGAT 
GGCTCATACG AGGATCTGCG ACCCACGGGA CAGAAGAACA AACAAAAGGT TCTCTCAGTG 
ATCGATGGAC GCCGAGCCCA AAACTGCACG ATTCTGTTGA GCAAACTGAA GATGAGCGAT 
GTGGAGATAT CCAAGGCCAT CCTCTCCATG GACTGCAATG AGCAGCTGCA ATTGGACATG 
GTCGAGCAGC TGTTGAAATT CACGCCCTCT GCCGAAGAGC GGGCTTTGCT GGACGAGCAC 
AGCGAGGACA TTGAATCGCT GGCACGAGCC GATCGTTTTC TCTATGAGAT ATCCAAGATA 
CCGCACTACG AGCAGCGGCT GAAGAGTCTG CACTACAAGA AGCGCTTTAT GCTGACCGTC 
AACGATCTGA CGCCGCGCAT CAAGAGCGTG ATGGAGGCCT CACGCGAGGT GGCACGCTCT 
CGGCGTCTGC GCAAGTTATT GGAGCTAGTC CTGGCATTGG GCAACTACAT GAATCGGGGG 
GCACGCGGTA ATGCCTCTGG CTTCCGTTTG GCGTCGCTCA ATCGTCTGGC CGATACCAAA 
TCGAGTGCCG CCAAGGGCAC AACGCTGCTC CATTATCTCG TGCAGGTTAT CGAAAAGAAG 
TTCAAGGACC TGCTGAAGCT GGAGGATGAT ATACCGCATG TGCGCGGTGC CTCGAAGGTG 
TCGCTGGGCG AGATGGACAA GGACATACAG ATGCTGCGCA CCGGGCTGGC GGATGTGGCC 
CGTGAGATTG AGTTCCATCG GAGTTCGGGT CCCGCACAGC AGGGTGATCG CTTTCTGCCC 
GTGATGCGGG AGTTCCATGC CCAGGCATCG GTGCGCTTTG CCGAGCTGGA GGACAAGTTC 
CAGGACATGA AGACGCGCTT CGATCGTGCC GTCCGTCTGT TCGGTGAAGA TGGATCCGTG 
TTGCAGCCGG ACGAGTTTTT TGGCATCTTT GATTCGTTCT TGGGAGCCTT TGCCGAGGCG 
CGTCACGACA ACGAAAGCTT CCGCCGACGG CAGGAGGAGG AGGAGAAGCG GGCCAAGCAG 
GAGGCCGAGC TCAAGAAGCG GACCATCGAT CGCAAGAACA AGACCGGCCT CATGAGCAGT 
GTTGCACGCA ACCTCGGCCT AAAGTCCTCA TCCCCCACGA ATGGAGGCGA CTCCCCGGGC 
AAGGGTGGCG GCGACAACAA GGGCGAGTTC GATGACCTTA TCTCGGCCCT GCGCACGGGC 
GATGTCTTTG GCGAGGACAT GGCCAAGTTT AAGCGATCGC GCAAGGCGCG TGTACTAAAT 
GGTGGATCAG GAGCGGGAGG TGCCACCGGC AACACCTCAC CGCCAAGGCA TGGCAGCCTT 
CAGCGCGAGG AGAGCGGACG CGAACGGGAG CGCACTGTGA GGCGTCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA13122 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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