Dpse\GA19007 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA19007
Entrez Gene ID	4815161
Full Name	GA19007 gene product from transcript GA19007-RB
Synonyms	GA19007,dpse_GLEANR_793
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001354584.3	XP_001354620.3	uncharacterized protein, isoform C
XM_015186031.1	XP_015041517.1	uncharacterized protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA19007 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA19007 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe24335	XM_001354584.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA19007), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODe24706	XM_015186031.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA19007), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA19007 ( XM_001354584.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001354620.3 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA19007), mRNA.

Price & Availability↑

CloneID	ODe24335
Clone ID Related Accession (Same CDS sequence)	XM_001354584.3
Accession Version	XM_001354584.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589960). On Dec 22, 2015 this sequence version replaced XM_001354584.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAATCCG CACTTGCCGC ATTTGAAAGA GCACACTCGA GCAATTCACA TCCGGGAACT 
GCAATTCAGA AGATGTTCAG GCACACTCAT ATAACACCGT CACTTGCAGC TCAACTCGAG 
GCGGAAGCGG TGCTGGACAA CCGCCAGCTT CTGGCATTCC AAGTGCGTAG TCGGGACATT 
GACTGGCGCC AATATGTCCA GCCTCTGGAC ACGCGCATCC GCTCTGGCGG AGTGTACCTG 
GACAAGCAGG CAGACTTTGT CAGCAATCAT CGACGTGCTG TCGACGACCA GAACAGGCGC 
GAGCTGCTCG TCTGCCACGA CATGATGGGC AACTACCTGG AGGATCGCCA CTTCCACAGC 
TCGGAGAAGT ACGATGACTA TCGCTTTCTC CACTGGTCAG CAGTGGACTA CTTCTGCTAC 
TTCAGCCATA AGTACGTGAC CATTCCGCCC AGCGGCTGGC TGAACGCCGC CCATCGACAT 
GGCGTTCCCG TTTTGGGTAC CTACATTGTG GAGGCAGATA GCCTTCTCGT CGAGGTCCTG 
GCCAGCGAGG AGAGCGTGGA CCGAACCGTG GCCGCCTTGA CCCGTCTCTG TCTCCACTTC 
GGCTTTGAGG GCTGGCTGGT GAACGTCGAA GTGACTGTAC CGCAGACAAG CATGCCCAAT 
CTGCATCGAT TTGTCCGAGA GCTGCGCTCT GCCACTGAGT CGCAGGTGCC GCATGGTCGT 
GTCTTTTGGT ACGACAGCGT GATCGAAACC GGAGCGTTGC TTTGGCAGAA CGAGCTGAAC 
GCACGGAACG TGGACTTCTT CCGGATCAGC GATGCCATGC TGATCAACTA TGCCTGGAAC 
GATGGCCACC TGGAGCGCAG TGCCAGCCTG GCCAGGCAGG AGGGTTCGCC CCAGCATCGA 
GTCTTCATGG GGCTGGATGT TTTCGGGCGG AGTCGGAAGG GCGGCTTCCA GAGCCTGGAC 
ACGATGGAGC GGATTGCCCA GAACGGTTTC TCGGCGGGTA TCTTTGCGCC CGCCTGGTCC 
TTCGAGACGC TGCGAAACTG GCAAGGCTAC GACATTAGCA AGGCGAATGG CGACGAGGAG 
GTGAACGCCG CCTTTCTGAG CAGAAACGAG TCCTGGTGGG CCCGCCTCTG GCCCCTGCTG 
GCCACCCACC CGTACCAGGC GCTGCCATTC TACACGGACT TCTGCGTGGG GTCAGGCCAG 
GCCAGCTTTG TGCGCGGGTC GCGTGATGCC TCGACGACGG GCCGACCCTT CTTTAACCTC 
TCCCGCCAAT CGCTGCAGCC GTCGGTGCCC CTGGACAGGA ATGCCCGGCA CTGTTTCCAC 
ACGGCCTTCA CGGGCGGCAG TTCTCTGGAG GTGATCAACT ACGAGCGTGC CTTCCGGCTC 
TTCGTCACAG ACTTCGTCCT GCCACGGGGT GTGCTGCTGC TGGGCTATGC CTACCGCATG 
GATGATGCCA GCAGAGATCA CACCTTTGAT GTGGTCGTGA GGCTCTCGCC CTTCCAACGG 
CATAGCCAGG ATTTGTACTT GTTCTGCGGC TCCTACAACA CGAACATTGT GGCTCCGCAT 
CTTTGCTACA TATCCCCAAT GGACGATCCA CTGCCCTCCG GCCTGGCCCC CGTACAACTG 
CGTCAGAGTT CCGGTCTGCT GGGTGACGGC TGGCGAGTGC GCTACTATAT GGCTCGGTTC 
GATGGACCCG TCGTGGTGAA GGACATTGGA CTGAAGTGCC GGCGACCGTT GGCGTCCACA 
TCGGACGCCT ATCTGGGGGC CATCTATGTG CAGGGCCTGT TCCAGGAGGA TTGGATGGCC 
ACGCAGTCCA GCCAGAAGGC TAGCATCGAT GTTTATGGCC GCCAGTTATG GGAGAACCTA 
ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001354620.3
CDS	1714..3579
Translation	MQSALAAFERAHSSNSHPGTAIQKMFRHTHITPSLAAQLEAEAVLDNRQLLAFQVRSRDIDWRQYVQPLDTRIRSGGVYLDKQADFVSNHRRAVDDQNRRELLVCHDMMGNYLEDRHFHSSEKYDDYRFLHWSAVDYFCYFSHKYVTIPPSGWLNAAHRHGVPVLGTYIVEADSLLVEVLASEESVDRTVAALTRLCLHFGFEGWLVNVEVTVPQTSMPNLHRFVRELRSATESQVPHGRVFWYDSVIETGALLWQNELNARNVDFFRISDAMLINYAWNDGHLERSASLARQEGSPQHRVFMGLDVFGRSRKGGFQSLDTMERIAQNGFSAGIFAPAWSFETLRNWQGYDISKANGDEEVNAAFLSRNESWWARLWPLLATHPYQALPFYTDFCVGSGQASFVRGSRDASTTGRPFFNLSRQSLQPSVPLDRNARHCFHTAFTGGSSLEVINYERAFRLFVTDFVLPRGVLLLGYAYRMDDASRDHTFDVVVRLSPFQRHSQDLYLFCGSYNTNIVAPHLCYISPMDDPLPSGLAPVQLRQSSGLLGDGWRVRYYMARFDGPVVVKDIGLKCRRPLASTSDAYLGAIYVQGLFQEDWMATQSSQKASIDVYGRQLWENLT

Target ORF information:

RefSeq Version	XM_001354584.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA19007), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001354584.3

ORF Insert Sequence:

ATGCAATCCG CACTTGCCGC ATTTGAAAGA GCACACTCGA GCAATTCACA TCCGGGAACT 
GCAATTCAGA AGATGTTCAG GCACACTCAT ATAACACCGT CACTTGCAGC TCAACTCGAG 
GCGGAAGCGG TGCTGGACAA CCGCCAGCTT CTGGCATTCC AAGTGCGTAG TCGGGACATT 
GACTGGCGCC AATATGTCCA GCCTCTGGAC ACGCGCATCC GCTCTGGCGG AGTGTACCTG 
GACAAGCAGG CAGACTTTGT CAGCAATCAT CGACGTGCTG TCGACGACCA GAACAGGCGC 
GAGCTGCTCG TCTGCCACGA CATGATGGGC AACTACCTGG AGGATCGCCA CTTCCACAGC 
TCGGAGAAGT ACGATGACTA TCGCTTTCTC CACTGGTCAG CAGTGGACTA CTTCTGCTAC 
TTCAGCCATA AGTACGTGAC CATTCCGCCC AGCGGCTGGC TGAACGCCGC CCATCGACAT 
GGCGTTCCCG TTTTGGGTAC CTACATTGTG GAGGCAGATA GCCTTCTCGT CGAGGTCCTG 
GCCAGCGAGG AGAGCGTGGA CCGAACCGTG GCCGCCTTGA CCCGTCTCTG TCTCCACTTC 
GGCTTTGAGG GCTGGCTGGT GAACGTCGAA GTGACTGTAC CGCAGACAAG CATGCCCAAT 
CTGCATCGAT TTGTCCGAGA GCTGCGCTCT GCCACTGAGT CGCAGGTGCC GCATGGTCGT 
GTCTTTTGGT ACGACAGCGT GATCGAAACC GGAGCGTTGC TTTGGCAGAA CGAGCTGAAC 
GCACGGAACG TGGACTTCTT CCGGATCAGC GATGCCATGC TGATCAACTA TGCCTGGAAC 
GATGGCCACC TGGAGCGCAG TGCCAGCCTG GCCAGGCAGG AGGGTTCGCC CCAGCATCGA 
GTCTTCATGG GGCTGGATGT TTTCGGGCGG AGTCGGAAGG GCGGCTTCCA GAGCCTGGAC 
ACGATGGAGC GGATTGCCCA GAACGGTTTC TCGGCGGGTA TCTTTGCGCC CGCCTGGTCC 
TTCGAGACGC TGCGAAACTG GCAAGGCTAC GACATTAGCA AGGCGAATGG CGACGAGGAG 
GTGAACGCCG CCTTTCTGAG CAGAAACGAG TCCTGGTGGG CCCGCCTCTG GCCCCTGCTG 
GCCACCCACC CGTACCAGGC GCTGCCATTC TACACGGACT TCTGCGTGGG GTCAGGCCAG 
GCCAGCTTTG TGCGCGGGTC GCGTGATGCC TCGACGACGG GCCGACCCTT CTTTAACCTC 
TCCCGCCAAT CGCTGCAGCC GTCGGTGCCC CTGGACAGGA ATGCCCGGCA CTGTTTCCAC 
ACGGCCTTCA CGGGCGGCAG TTCTCTGGAG GTGATCAACT ACGAGCGTGC CTTCCGGCTC 
TTCGTCACAG ACTTCGTCCT GCCACGGGGT GTGCTGCTGC TGGGCTATGC CTACCGCATG 
GATGATGCCA GCAGAGATCA CACCTTTGAT GTGGTCGTGA GGCTCTCGCC CTTCCAACGG 
CATAGCCAGG ATTTGTACTT GTTCTGCGGC TCCTACAACA CGAACATTGT GGCTCCGCAT 
CTTTGCTACA TATCCCCAAT GGACGATCCA CTGCCCTCCG GCCTGGCCCC CGTACAACTG 
CGTCAGAGTT CCGGTCTGCT GGGTGACGGC TGGCGAGTGC GCTACTATAT GGCTCGGTTC 
GATGGACCCG TCGTGGTGAA GGACATTGGA CTGAAGTGCC GGCGACCGTT GGCGTCCACA 
TCGGACGCCT ATCTGGGGGC CATCTATGTG CAGGGCCTGT TCCAGGAGGA TTGGATGGCC 
ACGCAGTCCA GCCAGAAGGC TAGCATCGAT GTTTATGGCC GCCAGTTATG GGAGAACCTA 
ACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA19007 ( XM_015186031.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015041517.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA19007), mRNA.

Price & Availability↑

CloneID	ODe24706
Clone ID Related Accession (Same CDS sequence)	XM_015186031.1
Accession Version	XM_015186031.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589960). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAGCAC CTGTGGATGC CGTACGTTCT ACTACATACA TTTGTACTTA TAAGCATACT 
ACATACATAC ATATGTATGT ATGTACATGC ATGTACCTTC TAAGGTGCGT TTCGAAGCTA 
TACGACACCA AAGAAATTGT AAAACAAAGA TTATTGTGCC ATTTGCGAGT TGGATTACAT 
TTCCTTCATT ACATATCTCA ACTCGAGGCG GAAGCGGTGC TGGACAACCG CCAGCTTCTG 
GCATTCCAAG TGCGTAGTCG GGACATTGAC TGGCGCCAAT ATGTCCAGCC TCTGGACACG 
CGCATCCGCT CTGGCGGAGT GTACCTGGAC AAGCAGGCAG ACTTTGTCAG CAATCATCGA 
CGTGCTGTCG ACGACCAGAA CAGGCGCGAG CTGCTCGTCT GCCACGACAT GATGGGCAAC 
TACCTGGAGG ATCGCCACTT CCACAGCTCG GAGAAGTACG ATGACTATCG CTTTCTCCAC 
TGGTCAGCAG TGGACTACTT CTGCTACTTC AGCCATAAGT ACGTGACCAT TCCGCCCAGC 
GGCTGGCTGA ACGCCGCCCA TCGACATGGC GTTCCCGTTT TGGGTACCTA CATTGTGGAG 
GCAGATAGCC TTCTCGTCGA GGTCCTGGCC AGCGAGGAGA GCGTGGACCG AACCGTGGCC 
GCCTTGACCC GTCTCTGTCT CCACTTCGGC TTTGAGGGCT GGCTGGTGAA CGTCGAAGTG 
ACTGTACCGC AGACAAGCAT GCCCAATCTG CATCGATTTG TCCGAGAGCT GCGCTCTGCC 
ACTGAGTCGC AGGTGCCGCA TGGTCGTGTC TTTTGGTACG ACAGCGTGAT CGAAACCGGA 
GCGTTGCTTT GGCAGAACGA GCTGAACGCA CGGAACGTGG ACTTCTTCCG GATCAGCGAT 
GCCATGCTGA TCAACTATGC CTGGAACGAT GGCCACCTGG AGCGCAGTGC CAGCCTGGCC 
AGGCAGGAGG GTTCGCCCCA GCATCGAGTC TTCATGGGGC TGGATGTTTT CGGGCGGAGT 
CGGAAGGGCG GCTTCCAGAG CCTGGACACG ATGGAGCGGA TTGCCCAGAA CGGTTTCTCG 
GCGGGTATCT TTGCGCCCGC CTGGTCCTTC GAGACGCTGC GAAACTGGCA AGGCTACGAC 
ATTAGCAAGG CGAATGGCGA CGAGGAGGTG AACGCCGCCT TTCTGAGCAG AAACGAGTCC 
TGGTGGGCCC GCCTCTGGCC CCTGCTGGCC ACCCACCCGT ACCAGGCGCT GCCATTCTAC 
ACGGACTTCT GCGTGGGGTC AGGCCAGGCC AGCTTTGTGC GCGGGTCGCG TGATGCCTCG 
ACGACGGGCC GACCCTTCTT TAACCTCTCC CGCCAATCGC TGCAGCCGTC GGTGCCCCTG 
GACAGGAATG CCCGGCACTG TTTCCACACG GCCTTCACGG GCGGCAGTTC TCTGGAGGTG 
ATCAACTACG AGCGTGCCTT CCGGCTCTTC GTCACAGACT TCGTCCTGCC ACGGGGTGTG 
CTGCTGCTGG GCTATGCCTA CCGCATGGAT GATGCCAGCA GAGATCACAC CTTTGATGTG 
GTCGTGAGGC TCTCGCCCTT CCAACGGCAT AGCCAGGATT TGTACTTGTT CTGCGGCTCC 
TACAACACGA ACATTGTGGC TCCGCATCTT TGCTACATAT CCCCAATGGA CGATCCACTG 
CCCTCCGGCC TGGCCCCCGT ACAACTGCGT CAGAGTTCCG GTCTGCTGGG TGACGGCTGG 
CGAGTGCGCT ACTATATGGC TCGGTTCGAT GGACCCGTCG TGGTGAAGGA CATTGGACTG 
AAGTGCCGGC GACCGTTGGC GTCCACATCG GACGCCTATC TGGGGGCCAT CTATGTGCAG 
GGCCTGTTCC AGGAGGATTG GATGGCCACG CAGTCCAGCC AGAAGGCTAG CATCGATGTT 
TATGGCCGCC AGTTATGGGA GAACCTAACC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015041517.1
CDS	96..2048
Translation	MPAPVDAVRSTTYICTYKHTTYIHMYVCTCMYLLRCVSKLYDTKEIVKQRLLCHLRVGLHFLHYISQLEAEAVLDNRQLLAFQVRSRDIDWRQYVQPLDTRIRSGGVYLDKQADFVSNHRRAVDDQNRRELLVCHDMMGNYLEDRHFHSSEKYDDYRFLHWSAVDYFCYFSHKYVTIPPSGWLNAAHRHGVPVLGTYIVEADSLLVEVLASEESVDRTVAALTRLCLHFGFEGWLVNVEVTVPQTSMPNLHRFVRELRSATESQVPHGRVFWYDSVIETGALLWQNELNARNVDFFRISDAMLINYAWNDGHLERSASLARQEGSPQHRVFMGLDVFGRSRKGGFQSLDTMERIAQNGFSAGIFAPAWSFETLRNWQGYDISKANGDEEVNAAFLSRNESWWARLWPLLATHPYQALPFYTDFCVGSGQASFVRGSRDASTTGRPFFNLSRQSLQPSVPLDRNARHCFHTAFTGGSSLEVINYERAFRLFVTDFVLPRGVLLLGYAYRMDDASRDHTFDVVVRLSPFQRHSQDLYLFCGSYNTNIVAPHLCYISPMDDPLPSGLAPVQLRQSSGLLGDGWRVRYYMARFDGPVVVKDIGLKCRRPLASTSDAYLGAIYVQGLFQEDWMATQSSQKASIDVYGRQLWENLT

Target ORF information:

RefSeq Version	XM_015186031.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA19007), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015186031.1

ORF Insert Sequence:

ATGCCAGCAC CTGTGGATGC CGTACGTTCT ACTACATACA TTTGTACTTA TAAGCATACT 
ACATACATAC ATATGTATGT ATGTACATGC ATGTACCTTC TAAGGTGCGT TTCGAAGCTA 
TACGACACCA AAGAAATTGT AAAACAAAGA TTATTGTGCC ATTTGCGAGT TGGATTACAT 
TTCCTTCATT ACATATCTCA ACTCGAGGCG GAAGCGGTGC TGGACAACCG CCAGCTTCTG 
GCATTCCAAG TGCGTAGTCG GGACATTGAC TGGCGCCAAT ATGTCCAGCC TCTGGACACG 
CGCATCCGCT CTGGCGGAGT GTACCTGGAC AAGCAGGCAG ACTTTGTCAG CAATCATCGA 
CGTGCTGTCG ACGACCAGAA CAGGCGCGAG CTGCTCGTCT GCCACGACAT GATGGGCAAC 
TACCTGGAGG ATCGCCACTT CCACAGCTCG GAGAAGTACG ATGACTATCG CTTTCTCCAC 
TGGTCAGCAG TGGACTACTT CTGCTACTTC AGCCATAAGT ACGTGACCAT TCCGCCCAGC 
GGCTGGCTGA ACGCCGCCCA TCGACATGGC GTTCCCGTTT TGGGTACCTA CATTGTGGAG 
GCAGATAGCC TTCTCGTCGA GGTCCTGGCC AGCGAGGAGA GCGTGGACCG AACCGTGGCC 
GCCTTGACCC GTCTCTGTCT CCACTTCGGC TTTGAGGGCT GGCTGGTGAA CGTCGAAGTG 
ACTGTACCGC AGACAAGCAT GCCCAATCTG CATCGATTTG TCCGAGAGCT GCGCTCTGCC 
ACTGAGTCGC AGGTGCCGCA TGGTCGTGTC TTTTGGTACG ACAGCGTGAT CGAAACCGGA 
GCGTTGCTTT GGCAGAACGA GCTGAACGCA CGGAACGTGG ACTTCTTCCG GATCAGCGAT 
GCCATGCTGA TCAACTATGC CTGGAACGAT GGCCACCTGG AGCGCAGTGC CAGCCTGGCC 
AGGCAGGAGG GTTCGCCCCA GCATCGAGTC TTCATGGGGC TGGATGTTTT CGGGCGGAGT 
CGGAAGGGCG GCTTCCAGAG CCTGGACACG ATGGAGCGGA TTGCCCAGAA CGGTTTCTCG 
GCGGGTATCT TTGCGCCCGC CTGGTCCTTC GAGACGCTGC GAAACTGGCA AGGCTACGAC 
ATTAGCAAGG CGAATGGCGA CGAGGAGGTG AACGCCGCCT TTCTGAGCAG AAACGAGTCC 
TGGTGGGCCC GCCTCTGGCC CCTGCTGGCC ACCCACCCGT ACCAGGCGCT GCCATTCTAC 
ACGGACTTCT GCGTGGGGTC AGGCCAGGCC AGCTTTGTGC GCGGGTCGCG TGATGCCTCG 
ACGACGGGCC GACCCTTCTT TAACCTCTCC CGCCAATCGC TGCAGCCGTC GGTGCCCCTG 
GACAGGAATG CCCGGCACTG TTTCCACACG GCCTTCACGG GCGGCAGTTC TCTGGAGGTG 
ATCAACTACG AGCGTGCCTT CCGGCTCTTC GTCACAGACT TCGTCCTGCC ACGGGGTGTG 
CTGCTGCTGG GCTATGCCTA CCGCATGGAT GATGCCAGCA GAGATCACAC CTTTGATGTG 
GTCGTGAGGC TCTCGCCCTT CCAACGGCAT AGCCAGGATT TGTACTTGTT CTGCGGCTCC 
TACAACACGA ACATTGTGGC TCCGCATCTT TGCTACATAT CCCCAATGGA CGATCCACTG 
CCCTCCGGCC TGGCCCCCGT ACAACTGCGT CAGAGTTCCG GTCTGCTGGG TGACGGCTGG 
CGAGTGCGCT ACTATATGGC TCGGTTCGAT GGACCCGTCG TGGTGAAGGA CATTGGACTG 
AAGTGCCGGC GACCGTTGGC GTCCACATCG GACGCCTATC TGGGGGCCAT CTATGTGCAG 
GGCCTGTTCC AGGAGGATTG GATGGCCACG CAGTCCAGCC AGAAGGCTAG CATCGATGTT 
TATGGCCGCC AGTTATGGGA GAACCTAACC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA19007 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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