Dpse\GA14321 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA14321
Entrez Gene ID	4814103
Full Name	GA14321 gene product from transcript GA14321-RB
Synonyms	GA14321,dpse_GLEANR_1469
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001354211.3	XP_001354247.3	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA14321 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA14321 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe25454	XM_001354211.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA14321), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA14321 ( XM_001354211.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001354247.3 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA14321), mRNA.

Price & Availability↑

CloneID	ODe25454
Clone ID Related Accession (Same CDS sequence)	XM_001354211.3
Accession Version	XM_001354211.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589957). On Dec 22, 2015 this sequence version replaced XM_001354211.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGAGCGT CCCACCAGCT GAGAGCGTCC GATTTCGTTG ACTACGCTGC CGTCGTGGGA 
GTGGGAGTGA GAGGAGGAGT GGGACTGGGT CTAGGACTGG AATTGGGCGA AGGATCGTAT 
TACAGCAGCG ATCTAACGGC GTTTGTCGTG GAGCAGGAGC AGCGTCAGCA GCAAAGGGAA 
GCAGAGGCAG CGGCAGAAGC AGCAGCAGCA GCAGCGGCAG CGGCAGCAGA AGCGGCAGCG 
ACAATCAAGC GAAAGCGTCA AACAGATTGC GATTGCGGCT GCGTGGACTC GTACAACATC 
AGCCACGGCC CGGACAGCAG TGCAACAGCA GCAGCAGCAG GATCAGCAGC AGCAGCAGCA 
GGTGCCACAC CACACAGCAA CAGCCTCAAG ACGGCGCACA CAAAGGTTGC CACATCGGGG 
GGCCATGCCA ATACGCAGCC CCCCAGCAAG AGGTCGAGCA GCGGTGCCGA CGGCGACTAT 
CAGTTGGTGC AGCACGAGGT CCTGTATTCG CTTTCGGCGG AGTATGAGGT CCTCGAGTTC 
TTGGGACGTG GCACCTTCGG TCAGGTGGTC AAATGCTGGA AGCGTGGCAC CTCCGAGATC 
GTCGCTATCA AGATCCTGAA GAACCATCCG TCGTACGCCC GCCAGGGACA GATCGAGGTC 
TCCATTCTGT CGCGTCTGAG CCAGGAGAAT GCCGACGAGT TCAACTTTGT GCGTGCCTTC 
GAGTGCTTCC AGCACAAGAA CCACACGTGT CTGGTGTTCG AGATGCTCGA GCAGAACCTG 
TACGACTTCC TCAAGCAGAA CAAGTTCTCG CCGCTGCCTT TGAAGTACAT AAGACCCATT 
TTGGAGCAGG TACTGACGGC CCTGTTGAAG CTCAAGCAAT TGGGTCTGAT TCATGCCGAT 
CTGAAGCCCG AGAACATAAT GCTGGTCGAT CCCGTGCGGC AGCCTTACCG CGTGAAGGTG 
ATCGACTTCG GGAGCGCCTC GCATGTGAGC AAAACTGTGT GCAACACGTA TCTGCAGTCG 
CGCTACTATC GCGCCCCGGA GATCATCCTG GGGCTGCCGT TCTGCGAGGC CATCGACATG 
TGGTCGCTGG GCTGTGTGGT GGCCGAGCTC TTCCTGGGCT GGCCCCTGTA CCCCGGCAGC 
TCGGAGTTCG ACCAGATCAG GTACATCTCG CAGACGCAGG GGCTGCCCAC GGAGCACATG 
CTGAACAGCG CCTCGAAGAC CTCGAAGTTC TTCTACCGCG ACGTGGACTC GACGTACCCG 
TTCTGGCGCC TCAAGACCAC CGAGGAGCAC GAGGCGGAGA CCAACACGAA GAGCAAGGAG 
GCGAGGAAGT ACATCTTCAA CTGCCTGGAC GACATCGGGC AGGTGAATGT TCCCACGGAC 
CTGGAGGGCG GCCAGCTGCT GGCCGAGAAA ACCGATCGGC GGGAGTTCAT AGATCTCCTC 
AAGCGCATGC TGACGATCGA CCAGGAGCGA CGCCTTACCC CCGCAGAGGC GCTCAACCAT 
TCGTTCACGC GCCTCACGCA CCTCGTCGAC TATGTGTATT GCAACAACGT GAAGGCCTCT 
GTCCAGATGA TGGAAGTGTG TCGGCGCGGC GATTTCCACA CCGTACAACC TGCTTCGACG 
TTGGTCACCA ATTTCGTGCC CAGCAGCACC GAGAACATGA CATTTACGAT CAACAACCAG 
CTGACCAGCC AAGTGCAGCG CCTCGTGCGG GACGGCCGGC CCCTGGCCTA CGAGGGCCTC 
TACCAGATCT ACAACGGCCG CAGTGTGGCG CGCCAGTATC CGCAGGCGCG CACCGACAGC 
TTCCAGCATC AGTTGGTGTC GAACATCCTC TGTCCGCCGT CGTACCAGGC GATGGCCAGT 
CCCACGAAGC ACGTGGTGGT GGGCAGTGCC ACCATGCAGC CGCCGCTGCA GGTGCCGCCG 
CAGCAGTACG TGAACGTGCC GGTGCCAGTG TCGATGGTGG AGCCCACCTC GGGCCAGAGG 
ATGCTGCTGA CGAACCGCGT GCAGGCGAGT GGTGTGGCCT GGCCCCAGAC CGGGCGACAG 
ATGGCCCTGG TGCCCTCGTG GCCGCAGCAG GCGCCCGCCC ACTCGCTGAT TGTCGACTCG 
ACGCCGCTCT TCAACGTGGA GGAGATCTAT CCCAAGCATC ATCTCAATTT GCCACGCAAC 
GATCTCAAAA AGGAATCGCC GGCTCATCAT TTAGCCAAGG GCAACTCTTA TCGCGTGCCG 
CGTCATGAGA AGAAGGAGCA CCAGCAGCTG TCGCCAGTGA AGAAGCGCGT GAAGGAGAGC 
TCGCCGCCGC ACCAGCAGCG CTTCCAGCGA ACGGCCCACG TGTCGCCGCA GTACCACACG 
CATCACAACT GCAATTACGG CGGCAACTAC GGCAGTGGCG GCAGCGGCGC GGGCGGCTCG 
GTGCTGGCCT CATCCGGTGG GGGAGCCTCG GCGAGCAATA TTCCCAGCAG CTCGGGGGGA 
TCGCACCACC ATCATGGCGC CCCAGTGGCG CATGTGCAGC AGCCGTATAG CGCCTCGTCG 
GGCGGCCATC ATATTGTGAG CAATTGTCAG GCGAATGGAG GCTACCAGCA GCCGCCGTCG 
CACGCCCCAC ATCCGCAGCA GCAGCAGCAC CAGCCGCAGC AGCAGCAACA GCATCCGCAT 
CCGCATCCAC ATCCGCATCC ACAGCAGCAG CAGCAGTCGC ATGTGAAGCA ACAGCCGACG 
ATCACCATCC ATGATACGCC CTCACCGACG GCAGTGATCA CCATTTCGGA CAGCGAGGAC 
GAGGGCGGGG ACAATGGGGC AACAACAGCG GCGCCAGTGA AGCAGCGGGC GCATGCCCAG 
TCCCAGACGA GCAGCAGTCT GATGCAGCAG CAGCAGCAGC AGCAATCCTC TGTGCCCCTG 
CATTGCCAGA GGAGCAGCAA CCAGAGCAAC GTGCAACAGC AGCAGCAACA GCAGCAGCAG 
CAGCAGCAGC AATCGATCAA TTATGGTGAA CACGATCCGG AGGACGCACG TCGACGCCAT 
CACGCAGCAG CCGCGGCAGC CATTGCCGCA GGGAGCCCCA AGCATCACCA TCAGCAGCAG 
CAACAACAAC AACAACAGCA ACAACACCAG CCGCCGCCAC AGCAGCAGCA GCCACACTAT 
CAGCCGCAGC CACAGCAGCA GCAACAGCAG CAGCAGCAGC AAGTGCAACA GCAGCCGCAG 
CCGAACATCA AGTACGAGCC AGGGCAATCG CAAAAGAAAC GCATCCTAGC GATGGCCCAA 
AGCGAGTGCA ACTACCAGCC GCAGCAGCAG GTGCAAGTGC AGCAGCCGCA GCCATCCGCC 
AGTCTGCCGC ACATTCCGCC AAAGCAGGAG CCGGCTGAGT TCTATCCGGA ATACGTCCCA 
CAGCAGCAGC AACAGCAGCA GTTGGACACG AAGCGCTCCT CGTGGGCGCC CACATCCTCC 
TCTGCTGCAC CGCCGCTGGC GCATCCAAAG CGAGAGGCGC CCTCGGCTGC GCCCATTAGC 
TATGTGGCAC CCGCAGTGGC GCCGCCCCTG GCCCACTCGA AGAGCAGTTC GTCGTCGTCT 
TCATCGTCGA TTAGCGCTGC GACTGCAGCG GCCGCAGCTG CAGCTGCCGC GGCGGCAGCC 
GCTGTGGGAC CGCCCTCCTG GGGGGCACCT CAGGTCTACC GCCAGCCATC GCAGCCGCCG 
CCAGGCAGTG TGGCCTCCCA GCCGCCGGCT GCGCCGCATC ACCACCACAG CAGCCACAGC 
CATCATCATC ATCAGGCTGG GACACCGCTG GGCGGCTCGC CCTCGTCCAC GGCAGCGGCG 
GCCATGCTGC AGACGGACAT CTATGCGCAG GGCGACATCT ATCGCCGTCC CACGGTGTTC 
GTGTCGCAGG CGGCGCCCAG CTACGCGTAC GCCAACAGAG CAGTGGTGGC CCCACCACCG 
GCGCACAACA GCAGCAGCCG TCAGGTGATA CCGTCGCATC CGTTGCCGGC CCACATCCAG 
ATACCCACGC AGTACAGCCA ATTCGGACCA CTCAGTCCTG CCCAGGTAGC TGCCGCCGGC 
AAGCATGCGG CGCACTTTGC GCCCACCAAC ATATGGTATG GGGCTGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001354247.3
CDS	414..4484
Translation	MRASHQLRASDFVDYAAVVGVGVRGGVGLGLGLELGEGSYYSSDLTAFVVEQEQRQQQREAEAAAEAAAAAAAAAAEAAATIKRKRQTDCDCGCVDSYNISHGPDSSATAAAAGSAAAAAGATPHSNSLKTAHTKVATSGGHANTQPPSKRSSSGADGDYQLVQHEVLYSLSAEYEVLEFLGRGTFGQVVKCWKRGTSEIVAIKILKNHPSYARQGQIEVSILSRLSQENADEFNFVRAFECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLPLKYIRPILEQVLTALLKLKQLGLIHADLKPENIMLVDPVRQPYRVKVIDFGSASHVSKTVCNTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVVAELFLGWPLYPGSSEFDQIRYISQTQGLPTEHMLNSASKTSKFFYRDVDSTYPFWRLKTTEEHEAETNTKSKEARKYIFNCLDDIGQVNVPTDLEGGQLLAEKTDRREFIDLLKRMLTIDQERRLTPAEALNHSFTRLTHLVDYVYCNNVKASVQMMEVCRRGDFHTVQPASTLVTNFVPSSTENMTFTINNQLTSQVQRLVRDGRPLAYEGLYQIYNGRSVARQYPQARTDSFQHQLVSNILCPPSYQAMASPTKHVVVGSATMQPPLQVPPQQYVNVPVPVSMVEPTSGQRMLLTNRVQASGVAWPQTGRQMALVPSWPQQAPAHSLIVDSTPLFNVEEIYPKHHLNLPRNDLKKESPAHHLAKGNSYRVPRHEKKEHQQLSPVKKRVKESSPPHQQRFQRTAHVSPQYHTHHNCNYGGNYGSGGSGAGGSVLASSGGGASASNIPSSSGGSHHHHGAPVAHVQQPYSASSGGHHIVSNCQANGGYQQPPSHAPHPQQQQHQPQQQQQHPHPHPHPHPQQQQQSHVKQQPTITIHDTPSPTAVITISDSEDEGGDNGATTAAPVKQRAHAQSQTSSSLMQQQQQQQSSVPLHCQRSSNQSNVQQQQQQQQQQQQQSINYGEHDPEDARRRHHAAAAAAIAAGSPKHHHQQQQQQQQQQQHQPPPQQQQPHYQPQPQQQQQQQQQQVQQQPQPNIKYEPGQSQKKRILAMAQSECNYQPQQQVQVQQPQPSASLPHIPPKQEPAEFYPEYVPQQQQQQQLDTKRSSWAPTSSSAAPPLAHPKREAPSAAPISYVAPAVAPPLAHSKSSSSSSSSSISAATAAAAAAAAAAAAAVGPPSWGAPQVYRQPSQPPPGSVASQPPAAPHHHHSSHSHHHHQAGTPLGGSPSSTAAAAMLQTDIYAQGDIYRRPTVFVSQAAPSYAYANRAVVAPPPAHNSSSRQVIPSHPLPAHIQIPTQYSQFGPLSPAQVAAAGKHAAHFAPTNIWYGAE

Target ORF information:

RefSeq Version	XM_001354211.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA14321), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001354211.3

ORF Insert Sequence:

ATGAGAGCGT CCCACCAGCT GAGAGCGTCC GATTTCGTTG ACTACGCTGC CGTCGTGGGA 
GTGGGAGTGA GAGGAGGAGT GGGACTGGGT CTAGGACTGG AATTGGGCGA AGGATCGTAT 
TACAGCAGCG ATCTAACGGC GTTTGTCGTG GAGCAGGAGC AGCGTCAGCA GCAAAGGGAA 
GCAGAGGCAG CGGCAGAAGC AGCAGCAGCA GCAGCGGCAG CGGCAGCAGA AGCGGCAGCG 
ACAATCAAGC GAAAGCGTCA AACAGATTGC GATTGCGGCT GCGTGGACTC GTACAACATC 
AGCCACGGCC CGGACAGCAG TGCAACAGCA GCAGCAGCAG GATCAGCAGC AGCAGCAGCA 
GGTGCCACAC CACACAGCAA CAGCCTCAAG ACGGCGCACA CAAAGGTTGC CACATCGGGG 
GGCCATGCCA ATACGCAGCC CCCCAGCAAG AGGTCGAGCA GCGGTGCCGA CGGCGACTAT 
CAGTTGGTGC AGCACGAGGT CCTGTATTCG CTTTCGGCGG AGTATGAGGT CCTCGAGTTC 
TTGGGACGTG GCACCTTCGG TCAGGTGGTC AAATGCTGGA AGCGTGGCAC CTCCGAGATC 
GTCGCTATCA AGATCCTGAA GAACCATCCG TCGTACGCCC GCCAGGGACA GATCGAGGTC 
TCCATTCTGT CGCGTCTGAG CCAGGAGAAT GCCGACGAGT TCAACTTTGT GCGTGCCTTC 
GAGTGCTTCC AGCACAAGAA CCACACGTGT CTGGTGTTCG AGATGCTCGA GCAGAACCTG 
TACGACTTCC TCAAGCAGAA CAAGTTCTCG CCGCTGCCTT TGAAGTACAT AAGACCCATT 
TTGGAGCAGG TACTGACGGC CCTGTTGAAG CTCAAGCAAT TGGGTCTGAT TCATGCCGAT 
CTGAAGCCCG AGAACATAAT GCTGGTCGAT CCCGTGCGGC AGCCTTACCG CGTGAAGGTG 
ATCGACTTCG GGAGCGCCTC GCATGTGAGC AAAACTGTGT GCAACACGTA TCTGCAGTCG 
CGCTACTATC GCGCCCCGGA GATCATCCTG GGGCTGCCGT TCTGCGAGGC CATCGACATG 
TGGTCGCTGG GCTGTGTGGT GGCCGAGCTC TTCCTGGGCT GGCCCCTGTA CCCCGGCAGC 
TCGGAGTTCG ACCAGATCAG GTACATCTCG CAGACGCAGG GGCTGCCCAC GGAGCACATG 
CTGAACAGCG CCTCGAAGAC CTCGAAGTTC TTCTACCGCG ACGTGGACTC GACGTACCCG 
TTCTGGCGCC TCAAGACCAC CGAGGAGCAC GAGGCGGAGA CCAACACGAA GAGCAAGGAG 
GCGAGGAAGT ACATCTTCAA CTGCCTGGAC GACATCGGGC AGGTGAATGT TCCCACGGAC 
CTGGAGGGCG GCCAGCTGCT GGCCGAGAAA ACCGATCGGC GGGAGTTCAT AGATCTCCTC 
AAGCGCATGC TGACGATCGA CCAGGAGCGA CGCCTTACCC CCGCAGAGGC GCTCAACCAT 
TCGTTCACGC GCCTCACGCA CCTCGTCGAC TATGTGTATT GCAACAACGT GAAGGCCTCT 
GTCCAGATGA TGGAAGTGTG TCGGCGCGGC GATTTCCACA CCGTACAACC TGCTTCGACG 
TTGGTCACCA ATTTCGTGCC CAGCAGCACC GAGAACATGA CATTTACGAT CAACAACCAG 
CTGACCAGCC AAGTGCAGCG CCTCGTGCGG GACGGCCGGC CCCTGGCCTA CGAGGGCCTC 
TACCAGATCT ACAACGGCCG CAGTGTGGCG CGCCAGTATC CGCAGGCGCG CACCGACAGC 
TTCCAGCATC AGTTGGTGTC GAACATCCTC TGTCCGCCGT CGTACCAGGC GATGGCCAGT 
CCCACGAAGC ACGTGGTGGT GGGCAGTGCC ACCATGCAGC CGCCGCTGCA GGTGCCGCCG 
CAGCAGTACG TGAACGTGCC GGTGCCAGTG TCGATGGTGG AGCCCACCTC GGGCCAGAGG 
ATGCTGCTGA CGAACCGCGT GCAGGCGAGT GGTGTGGCCT GGCCCCAGAC CGGGCGACAG 
ATGGCCCTGG TGCCCTCGTG GCCGCAGCAG GCGCCCGCCC ACTCGCTGAT TGTCGACTCG 
ACGCCGCTCT TCAACGTGGA GGAGATCTAT CCCAAGCATC ATCTCAATTT GCCACGCAAC 
GATCTCAAAA AGGAATCGCC GGCTCATCAT TTAGCCAAGG GCAACTCTTA TCGCGTGCCG 
CGTCATGAGA AGAAGGAGCA CCAGCAGCTG TCGCCAGTGA AGAAGCGCGT GAAGGAGAGC 
TCGCCGCCGC ACCAGCAGCG CTTCCAGCGA ACGGCCCACG TGTCGCCGCA GTACCACACG 
CATCACAACT GCAATTACGG CGGCAACTAC GGCAGTGGCG GCAGCGGCGC GGGCGGCTCG 
GTGCTGGCCT CATCCGGTGG GGGAGCCTCG GCGAGCAATA TTCCCAGCAG CTCGGGGGGA 
TCGCACCACC ATCATGGCGC CCCAGTGGCG CATGTGCAGC AGCCGTATAG CGCCTCGTCG 
GGCGGCCATC ATATTGTGAG CAATTGTCAG GCGAATGGAG GCTACCAGCA GCCGCCGTCG 
CACGCCCCAC ATCCGCAGCA GCAGCAGCAC CAGCCGCAGC AGCAGCAACA GCATCCGCAT 
CCGCATCCAC ATCCGCATCC ACAGCAGCAG CAGCAGTCGC ATGTGAAGCA ACAGCCGACG 
ATCACCATCC ATGATACGCC CTCACCGACG GCAGTGATCA CCATTTCGGA CAGCGAGGAC 
GAGGGCGGGG ACAATGGGGC AACAACAGCG GCGCCAGTGA AGCAGCGGGC GCATGCCCAG 
TCCCAGACGA GCAGCAGTCT GATGCAGCAG CAGCAGCAGC AGCAATCCTC TGTGCCCCTG 
CATTGCCAGA GGAGCAGCAA CCAGAGCAAC GTGCAACAGC AGCAGCAACA GCAGCAGCAG 
CAGCAGCAGC AATCGATCAA TTATGGTGAA CACGATCCGG AGGACGCACG TCGACGCCAT 
CACGCAGCAG CCGCGGCAGC CATTGCCGCA GGGAGCCCCA AGCATCACCA TCAGCAGCAG 
CAACAACAAC AACAACAGCA ACAACACCAG CCGCCGCCAC AGCAGCAGCA GCCACACTAT 
CAGCCGCAGC CACAGCAGCA GCAACAGCAG CAGCAGCAGC AAGTGCAACA GCAGCCGCAG 
CCGAACATCA AGTACGAGCC AGGGCAATCG CAAAAGAAAC GCATCCTAGC GATGGCCCAA 
AGCGAGTGCA ACTACCAGCC GCAGCAGCAG GTGCAAGTGC AGCAGCCGCA GCCATCCGCC 
AGTCTGCCGC ACATTCCGCC AAAGCAGGAG CCGGCTGAGT TCTATCCGGA ATACGTCCCA 
CAGCAGCAGC AACAGCAGCA GTTGGACACG AAGCGCTCCT CGTGGGCGCC CACATCCTCC 
TCTGCTGCAC CGCCGCTGGC GCATCCAAAG CGAGAGGCGC CCTCGGCTGC GCCCATTAGC 
TATGTGGCAC CCGCAGTGGC GCCGCCCCTG GCCCACTCGA AGAGCAGTTC GTCGTCGTCT 
TCATCGTCGA TTAGCGCTGC GACTGCAGCG GCCGCAGCTG CAGCTGCCGC GGCGGCAGCC 
GCTGTGGGAC CGCCCTCCTG GGGGGCACCT CAGGTCTACC GCCAGCCATC GCAGCCGCCG 
CCAGGCAGTG TGGCCTCCCA GCCGCCGGCT GCGCCGCATC ACCACCACAG CAGCCACAGC 
CATCATCATC ATCAGGCTGG GACACCGCTG GGCGGCTCGC CCTCGTCCAC GGCAGCGGCG 
GCCATGCTGC AGACGGACAT CTATGCGCAG GGCGACATCT ATCGCCGTCC CACGGTGTTC 
GTGTCGCAGG CGGCGCCCAG CTACGCGTAC GCCAACAGAG CAGTGGTGGC CCCACCACCG 
GCGCACAACA GCAGCAGCCG TCAGGTGATA CCGTCGCATC CGTTGCCGGC CCACATCCAG 
ATACCCACGC AGTACAGCCA ATTCGGACCA CTCAGTCCTG CCCAGGTAGC TGCCGCCGGC 
AAGCATGCGG CGCACTTTGC GCCCACCAAC ATATGGTATG GGGCTGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA14321 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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