Dpse\GA17396 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA17396
Entrez Gene ID	4812941
Full Name	GA17396 gene product from transcript GA17396-RB
Synonyms	GA17396,dpse_GLEANR_12722
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_015187121.1	XP_015042607.1	uncharacterized protein, isoform B
XM_001353154.3	XP_001353190.3	uncharacterized protein, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA17396 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA17396 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe25621	XM_001353154.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA17396), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODe26037	XM_015187121.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA17396), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA17396 ( XM_001353154.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001353190.3 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA17396), mRNA.

Price & Availability↑

CloneID	ODe25621
Clone ID Related Accession (Same CDS sequence)	XM_001353154.3
Accession Version	XM_001353154.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589955). On Dec 22, 2015 this sequence version replaced XM_001353154.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGGTTC GAAAAGGCTA TTTGGTGCTC CTCTTGGTTT TGGTAGCCAA AGAAACCAAA 
GTTTCGAGCA AAAAGGTTTC CAGTCTGGAG CCCAGAAGCT TGAACCAATC ACAGAAATTT 
GAGTACTGCG TCAAGATTCT TATGTTGTGC CACCTCATCT GGGATTCGCC GCCCGACTAC 
GAGGATTTTG ACAACGTGAA CGTGGATTCA ATGAAGTATG CTCACGATAA CAGTCACTTC 
AAGGATGATT ACAAGAAGGC CAAATGCATA CCTCCGGGCC TCAGTAAAAA AGATCTATTA 
GAGTTCTGGG TAGCTGTGGC CGAGTGGGAG ATGGAGGTGA GGAACCTTTT CCTCGTCGAG 
AAATTCCTGC CCATCATCAA GGAGTCCGAC AACGCCATTA CGATCAATCT GTGGGGGTTG 
CTCCACCTCC GAAAGTCAGT AATGTGCTAT CGAAAATTGG ACCCCAAAGC GACACCGACA 
CCGACACCGA CTCCAACATC AACAAGACAA CCCACGACAC AACTGACGAC GACATCCAAT 
GAAATTTCAT CAACATTGGC TTACCCTTCG ACTTCCAGCT GCAATGGGAA TACCGAAATC 
GGAATATGGA TTAATCAGCA GATCAATGGA AGTCTAGCTC TGGATAAACT AATCAAGAAA 
ATGTTGATAA TTGTGGGTAA GATCGAGCGT CACATACACA GAAAATGCTG TCCCTACGAG 
CCCATCCGCG ATAGGGTGGA AGTAGTCCAC GATGGCATCG ATTTCTTGCT GCAATTAAAC 
GGTATAGAGC CCTACAAGAG AGCGCAGTCC ACGGGGTATA TCGGCTCCGA CTTTGATAAT 
CTAAAGACGA GATTGGAGTA CCTGAGCCTT GCGCTAGCCA AGGGAGCAGC AAAGGGAAAT 
CGTTCCGTAG GAAAGTGCTG CCCCGCCTGG TCTGACCAAT ATAATACTTT CCAAGTTGAG 
ATGGAAGAAA ATTTGGAAAA GCTTGAAGCA AATAGCTCCG ATCTGACGGC ACATTTGACA 
AACTTGATCG ACAAGCAAGA GGCCTTGGAG ATGGATAGGT CAGATCAAGT GAAGCTTTTG 
CAAAGTCTAG ACGCTTTGCA TGGCAGTCCA GCTTCTGAGA GCACAAAAAG GTGTTGTCCG 
AATCAGAAAA AAGATATGGA AAATATAGCA AAGATATTGA AAGACTTGAA ATTAAGTGAC 
GGCCTGCCAG AGTCAATCGA GACAGCTGAC TCTCTTCAGG ATAGACTGAC TAAAGCGAAC 
GCAAATCTAA TAAGTGCGAA AGGCTTGCTG AACGATGAGG AGGCAAATAT CCGAAAAATG 
GCTTCTTTGT GCGGAGAGTT TTGTCCAAGT CAGAAGAAAG GAAATATCGA CGATCAATCG 
GCAAAAGTAA GCCAACTAGA AAAACAAATG AAGGAAATTA ATGATAAAGC TCCAGAGATA 
ATGGAACTTT TAAAGTCACC AAATTGGCCA GAACATCTGA AAGCTATAGA AACGGTAAAG 
CAAAAAGTCA AAGATTTTAT GACCAAGCAG AGACAGGATG ATGTGAACCA GCAATTGGCC 
ATGGTTCCTG AAGAGCCATC GGAGTCATTA AAAAATATGA CCCACAGCTT GGAAGCGCTG 
GAGGCAAAGC TGAGTGAACT CCGTCCCAAG AACCAGACCT ACGAACAGCT CCTGTTAGAC 
AGCATCGGAG GACTGGAGCA ACGTCTGCGA GACCGTCTGA GTGGCCTAAA CGCAATGATC 
ACTAAGACCG AAGCGGATGC AGATAAATGT AACTCTAAAT GCGACTGGGG GAATCTGCCG 
ACCATGGAGG AACTCCAGGA AAGGGTCAAG GAAATGGAGG CATTGGTTGT TACAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001353190.3
CDS	54..1913
Translation	MQVRKGYLVLLLVLVAKETKVSSKKVSSLEPRSLNQSQKFEYCVKILMLCHLIWDSPPDYEDFDNVNVDSMKYAHDNSHFKDDYKKAKCIPPGLSKKDLLEFWVAVAEWEMEVRNLFLVEKFLPIIKESDNAITINLWGLLHLRKSVMCYRKLDPKATPTPTPTPTSTRQPTTQLTTTSNEISSTLAYPSTSSCNGNTEIGIWINQQINGSLALDKLIKKMLIIVGKIERHIHRKCCPYEPIRDRVEVVHDGIDFLLQLNGIEPYKRAQSTGYIGSDFDNLKTRLEYLSLALAKGAAKGNRSVGKCCPAWSDQYNTFQVEMEENLEKLEANSSDLTAHLTNLIDKQEALEMDRSDQVKLLQSLDALHGSPASESTKRCCPNQKKDMENIAKILKDLKLSDGLPESIETADSLQDRLTKANANLISAKGLLNDEEANIRKMASLCGEFCPSQKKGNIDDQSAKVSQLEKQMKEINDKAPEIMELLKSPNWPEHLKAIETVKQKVKDFMTKQRQDDVNQQLAMVPEEPSESLKNMTHSLEALEAKLSELRPKNQTYEQLLLDSIGGLEQRLRDRLSGLNAMITKTEADADKCNSKCDWGNLPTMEELQERVKEMEALVVTK

Target ORF information:

RefSeq Version	XM_001353154.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA17396), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001353154.3

ORF Insert Sequence:

ATGCAGGTTC GAAAAGGCTA TTTGGTGCTC CTCTTGGTTT TGGTAGCCAA AGAAACCAAA 
GTTTCGAGCA AAAAGGTTTC CAGTCTGGAG CCCAGAAGCT TGAACCAATC ACAGAAATTT 
GAGTACTGCG TCAAGATTCT TATGTTGTGC CACCTCATCT GGGATTCGCC GCCCGACTAC 
GAGGATTTTG ACAACGTGAA CGTGGATTCA ATGAAGTATG CTCACGATAA CAGTCACTTC 
AAGGATGATT ACAAGAAGGC CAAATGCATA CCTCCGGGCC TCAGTAAAAA AGATCTATTA 
GAGTTCTGGG TAGCTGTGGC CGAGTGGGAG ATGGAGGTGA GGAACCTTTT CCTCGTCGAG 
AAATTCCTGC CCATCATCAA GGAGTCCGAC AACGCCATTA CGATCAATCT GTGGGGGTTG 
CTCCACCTCC GAAAGTCAGT AATGTGCTAT CGAAAATTGG ACCCCAAAGC GACACCGACA 
CCGACACCGA CTCCAACATC AACAAGACAA CCCACGACAC AACTGACGAC GACATCCAAT 
GAAATTTCAT CAACATTGGC TTACCCTTCG ACTTCCAGCT GCAATGGGAA TACCGAAATC 
GGAATATGGA TTAATCAGCA GATCAATGGA AGTCTAGCTC TGGATAAACT AATCAAGAAA 
ATGTTGATAA TTGTGGGTAA GATCGAGCGT CACATACACA GAAAATGCTG TCCCTACGAG 
CCCATCCGCG ATAGGGTGGA AGTAGTCCAC GATGGCATCG ATTTCTTGCT GCAATTAAAC 
GGTATAGAGC CCTACAAGAG AGCGCAGTCC ACGGGGTATA TCGGCTCCGA CTTTGATAAT 
CTAAAGACGA GATTGGAGTA CCTGAGCCTT GCGCTAGCCA AGGGAGCAGC AAAGGGAAAT 
CGTTCCGTAG GAAAGTGCTG CCCCGCCTGG TCTGACCAAT ATAATACTTT CCAAGTTGAG 
ATGGAAGAAA ATTTGGAAAA GCTTGAAGCA AATAGCTCCG ATCTGACGGC ACATTTGACA 
AACTTGATCG ACAAGCAAGA GGCCTTGGAG ATGGATAGGT CAGATCAAGT GAAGCTTTTG 
CAAAGTCTAG ACGCTTTGCA TGGCAGTCCA GCTTCTGAGA GCACAAAAAG GTGTTGTCCG 
AATCAGAAAA AAGATATGGA AAATATAGCA AAGATATTGA AAGACTTGAA ATTAAGTGAC 
GGCCTGCCAG AGTCAATCGA GACAGCTGAC TCTCTTCAGG ATAGACTGAC TAAAGCGAAC 
GCAAATCTAA TAAGTGCGAA AGGCTTGCTG AACGATGAGG AGGCAAATAT CCGAAAAATG 
GCTTCTTTGT GCGGAGAGTT TTGTCCAAGT CAGAAGAAAG GAAATATCGA CGATCAATCG 
GCAAAAGTAA GCCAACTAGA AAAACAAATG AAGGAAATTA ATGATAAAGC TCCAGAGATA 
ATGGAACTTT TAAAGTCACC AAATTGGCCA GAACATCTGA AAGCTATAGA AACGGTAAAG 
CAAAAAGTCA AAGATTTTAT GACCAAGCAG AGACAGGATG ATGTGAACCA GCAATTGGCC 
ATGGTTCCTG AAGAGCCATC GGAGTCATTA AAAAATATGA CCCACAGCTT GGAAGCGCTG 
GAGGCAAAGC TGAGTGAACT CCGTCCCAAG AACCAGACCT ACGAACAGCT CCTGTTAGAC 
AGCATCGGAG GACTGGAGCA ACGTCTGCGA GACCGTCTGA GTGGCCTAAA CGCAATGATC 
ACTAAGACCG AAGCGGATGC AGATAAATGT AACTCTAAAT GCGACTGGGG GAATCTGCCG 
ACCATGGAGG AACTCCAGGA AAGGGTCAAG GAAATGGAGG CATTGGTTGT TACAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA17396 ( XM_015187121.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015042607.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA17396), partial mRNA.

Price & Availability↑

CloneID	ODe26037
Clone ID Related Accession (Same CDS sequence)	XM_015187121.1
Accession Version	XM_015187121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589955). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGAAAGCAA TCAGGTGTAA ATCAAAGAAA CAGGCCCAGG CATGCAGTCA CGTCATGGTT 
CGAAAAGGCT ATTTGGTGCT CCTCTTGGTT TTGGTAGCCA AAGAAACCAA AGTTTCGAGC 
AAAAAGGTTT CCAGTCTGGA GCCCAGAAGC TTGAACCAAT CACAGAAATT TGAGTACTGC 
GTCAAGATTC TTATGTTGTG CCACCTCATC TGGGATTCGC CGCCCGACTA CGAGGATTTT 
GACAACGTGA ACGTGGATTC AATGAAGTAT GCTCACGATA ACAGTCACTT CAAGGATGAT 
TACAAGAAGG CCAAATGCAT ACCTCCGGGC CTCAGTAAAA AAGATCTATT AGAGTTCTGG 
GTAGCTGTGG CCGAGTGGGA GATGGAGGTG AGGAACCTTT TCCTCGTCGA GAAATTCCTG 
CCCATCATCA AGGAGTCCGA CAACGCCATT ACGATCAATC TGTGGGGGTT GCTCCACCTC 
CGAAAGTCAG TAATGTGCTA TCGAAAATTG GACCCCAAAG CGACACCGAC ACCGACACCG 
ACTCCAACAT CAACAAGACA ACCCACGACA CAACTGACGA CGACATCCAA TGAAATTTCA 
TCAACATTGG CTTACCCTTC GACTTCCAGC TGCAATGGGA ATACCGAAAT CGGAATATGG 
ATTAATCAGC AGATCAATGG AAGTCTAGCT CTGGATAAAC TAATCAAGAA AATGTTGATA 
ATTGTGGGTA AGATCGAGCG TCACATACAC AGAAAATGCT GTCCCTACGA GCCCATCCGC 
GATAGGGTGG AAGTAGTCCA CGATGGCATC GATTTCTTGC TGCAATTAAA CGGTATAGAG 
CCCTACAAGA GAGCGCAGTC CACGGGGTAT ATCGGCTCCG ACTTTGATAA TCTAAAGACG 
AGATTGGAGT ACCTGAGCCT TGCGCTAGCC AAGGGAGCAG CAAAGGGAAA TCGTTCCGTA 
GGAAAGTGCT GCCCCGCCTG GTCTGACCAA TATAATACTT TCCAAGTTGA GATGGAAGAA 
AATTTGGAAA AGCTTGAAGC AAATAGCTCC GATCTGACGG CACATTTGAC AAACTTGATC 
GACAAGCAAG AGGCCTTGGA GATGGATAGG TCAGATCAAG TGAAGCTTTT GCAAAGTCTA 
GACGCTTTGC ATGGCAGTCC AGCTTCTGAG AGCACAAAAA GGTGTTGTCC GAATCAGAAA 
AAAGATATGG AAAATATAGC AAAGATATTG AAAGACTTGA AATTAAGTGA CGGCCTGCCA 
GAGTCAATCG AGACAGCTGA CTCTCTTCAG GATAGACTGA CTAAAGCGAA CGCAAATCTA 
ATAAGTGCGA AAGGCTTGCT GAACGATGAG GAGGCAAATA TCCGAAAAAT GGCTTCTTTG 
TGCGGAGAGT TTTGTCCAAG TCAGAAGAAA GGAAATATCG ACGATCAATC GGCAAAAGTA 
AGCCAACTAG AAAAACAAAT GAAGGAAATT AATGATAAAG CTCCAGAGAT AATGGAACTT 
TTAAAGTCAC CAAATTGGCC AGAACATCTG AAAGCTATAG AAACGGTAAA GCAAAAAGTC 
AAAGATTTTA TGACCAAGCA GAGACAGGAT GATGTGAACC AGCAATTGGC CATGGTTCCT 
GAAGAGCCAT CGGAGTCATT AAAAAATATG ACCCACAGCT TGGAAGCGCT GGAGGCAAAG 
CTGAGTGAAC TCCGTCCCAA GAACCAGACC TACGAACAGC TCCTGTTAGA CAGCATCGGA 
GGACTGGAGC AACGTCTGCG AGACCGTCTG AGTGGCCTAA ACGCAATGAT CACTAAGACC 
GAAGCGGATG CAGATAAATG TAACTCTAAA TGCGACTGGG GGAATCTGCC GACCATGGAG 
GAACTCCAGG AAAGGGTCAA GGAAATGGAG GCATTGGTTG TTACAAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015042607.1
CDS	1..1911
Translation	MKAIRCKSKKQAQACSHVMVRKGYLVLLLVLVAKETKVSSKKVSSLEPRSLNQSQKFEYCVKILMLCHLIWDSPPDYEDFDNVNVDSMKYAHDNSHFKDDYKKAKCIPPGLSKKDLLEFWVAVAEWEMEVRNLFLVEKFLPIIKESDNAITINLWGLLHLRKSVMCYRKLDPKATPTPTPTPTSTRQPTTQLTTTSNEISSTLAYPSTSSCNGNTEIGIWINQQINGSLALDKLIKKMLIIVGKIERHIHRKCCPYEPIRDRVEVVHDGIDFLLQLNGIEPYKRAQSTGYIGSDFDNLKTRLEYLSLALAKGAAKGNRSVGKCCPAWSDQYNTFQVEMEENLEKLEANSSDLTAHLTNLIDKQEALEMDRSDQVKLLQSLDALHGSPASESTKRCCPNQKKDMENIAKILKDLKLSDGLPESIETADSLQDRLTKANANLISAKGLLNDEEANIRKMASLCGEFCPSQKKGNIDDQSAKVSQLEKQMKEINDKAPEIMELLKSPNWPEHLKAIETVKQKVKDFMTKQRQDDVNQQLAMVPEEPSESLKNMTHSLEALEAKLSELRPKNQTYEQLLLDSIGGLEQRLRDRLSGLNAMITKTEADADKCNSKCDWGNLPTMEELQERVKEMEALVVTK

Target ORF information:

RefSeq Version	XM_015187121.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA17396), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015187121.1

ORF Insert Sequence:

ATGAAAGCAA TCAGGTGTAA ATCAAAGAAA CAGGCCCAGG CATGCAGTCA CGTCATGGTT 
CGAAAAGGCT ATTTGGTGCT CCTCTTGGTT TTGGTAGCCA AAGAAACCAA AGTTTCGAGC 
AAAAAGGTTT CCAGTCTGGA GCCCAGAAGC TTGAACCAAT CACAGAAATT TGAGTACTGC 
GTCAAGATTC TTATGTTGTG CCACCTCATC TGGGATTCGC CGCCCGACTA CGAGGATTTT 
GACAACGTGA ACGTGGATTC AATGAAGTAT GCTCACGATA ACAGTCACTT CAAGGATGAT 
TACAAGAAGG CCAAATGCAT ACCTCCGGGC CTCAGTAAAA AAGATCTATT AGAGTTCTGG 
GTAGCTGTGG CCGAGTGGGA GATGGAGGTG AGGAACCTTT TCCTCGTCGA GAAATTCCTG 
CCCATCATCA AGGAGTCCGA CAACGCCATT ACGATCAATC TGTGGGGGTT GCTCCACCTC 
CGAAAGTCAG TAATGTGCTA TCGAAAATTG GACCCCAAAG CGACACCGAC ACCGACACCG 
ACTCCAACAT CAACAAGACA ACCCACGACA CAACTGACGA CGACATCCAA TGAAATTTCA 
TCAACATTGG CTTACCCTTC GACTTCCAGC TGCAATGGGA ATACCGAAAT CGGAATATGG 
ATTAATCAGC AGATCAATGG AAGTCTAGCT CTGGATAAAC TAATCAAGAA AATGTTGATA 
ATTGTGGGTA AGATCGAGCG TCACATACAC AGAAAATGCT GTCCCTACGA GCCCATCCGC 
GATAGGGTGG AAGTAGTCCA CGATGGCATC GATTTCTTGC TGCAATTAAA CGGTATAGAG 
CCCTACAAGA GAGCGCAGTC CACGGGGTAT ATCGGCTCCG ACTTTGATAA TCTAAAGACG 
AGATTGGAGT ACCTGAGCCT TGCGCTAGCC AAGGGAGCAG CAAAGGGAAA TCGTTCCGTA 
GGAAAGTGCT GCCCCGCCTG GTCTGACCAA TATAATACTT TCCAAGTTGA GATGGAAGAA 
AATTTGGAAA AGCTTGAAGC AAATAGCTCC GATCTGACGG CACATTTGAC AAACTTGATC 
GACAAGCAAG AGGCCTTGGA GATGGATAGG TCAGATCAAG TGAAGCTTTT GCAAAGTCTA 
GACGCTTTGC ATGGCAGTCC AGCTTCTGAG AGCACAAAAA GGTGTTGTCC GAATCAGAAA 
AAAGATATGG AAAATATAGC AAAGATATTG AAAGACTTGA AATTAAGTGA CGGCCTGCCA 
GAGTCAATCG AGACAGCTGA CTCTCTTCAG GATAGACTGA CTAAAGCGAA CGCAAATCTA 
ATAAGTGCGA AAGGCTTGCT GAACGATGAG GAGGCAAATA TCCGAAAAAT GGCTTCTTTG 
TGCGGAGAGT TTTGTCCAAG TCAGAAGAAA GGAAATATCG ACGATCAATC GGCAAAAGTA 
AGCCAACTAG AAAAACAAAT GAAGGAAATT AATGATAAAG CTCCAGAGAT AATGGAACTT 
TTAAAGTCAC CAAATTGGCC AGAACATCTG AAAGCTATAG AAACGGTAAA GCAAAAAGTC 
AAAGATTTTA TGACCAAGCA GAGACAGGAT GATGTGAACC AGCAATTGGC CATGGTTCCT 
GAAGAGCCAT CGGAGTCATT AAAAAATATG ACCCACAGCT TGGAAGCGCT GGAGGCAAAG 
CTGAGTGAAC TCCGTCCCAA GAACCAGACC TACGAACAGC TCCTGTTAGA CAGCATCGGA 
GGACTGGAGC AACGTCTGCG AGACCGTCTG AGTGGCCTAA ACGCAATGAT CACTAAGACC 
GAAGCGGATG CAGATAAATG TAACTCTAAA TGCGACTGGG GGAATCTGCC GACCATGGAG 
GAACTCCAGG AAAGGGTCAA GGAAATGGAG GCATTGGTTG TTACAAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA17396 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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