Dpse\GA18727 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA18727
Entrez Gene ID	4811878
Full Name	GA18727 gene product from transcript GA18727-RB
Synonyms	GA18727,dpse_GLEANR_7725
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001352947.3	XP_001352983.2	uncharacterized protein, isoform A
XM_015188578.1	XP_015044064.1	uncharacterized protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA18727 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA18727 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe07260	XM_015188578.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA18727), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODe07260	XM_001352947.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA18727), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA18727 ( XM_001352947.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001352983.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA18727), mRNA.

Price & Availability↑

CloneID	ODe07260
Clone ID Related Accession (Same CDS sequence)	XM_001352947.3 , XM_015188578.1
Accession Version	XM_001352947.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589954). On Dec 22, 2015 this sequence version replaced XM_001352947.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGATAC TCCAGATCTT ACTGCTGCTC TGTGCCGGGA CACTCCTGAC CAGGGCCGAC 
AGCTGTCCGC CCTCTCAGGC CATCCTGCCC TGTCGCTGCT CGCTGCGTGG CAAGGAGATA 
CAGATATGGT GCTCGCACAG CAATCTGCCC CAAATCATGG ACGGGCTGAA GGCCGTGGAG 
CGGAATATCA AGAGCCAAAT CGATGAGCTG GTGCTGGAGA ACAATCAGCT GCCGGCGTTG 
CCGGGACGCT TCTTTGGTAA CCTGCAGATT GTGCGGCTGA TGCTGCGCTA CAACAGCATC 
GAACGGGTGT CCAACGGCTG GCTGAACGAG CTGGAGAACA GCCTGGTGGA GATCTTCATC 
GTGGAGCCGC AGCTGCGGAG CATTCCCGCG GAGAGTCTCA ATGGCATGAT CAATATGCTG 
GCCATAACCA TACAGAGCGA CGAGCTGAAG CATCTGCCGG ACTTCTCGGG CCTGCTCAGC 
CTCACCTACC TGAGTGTGCA GACTGGATCC CTGCTGGAGC TGGCGCCCCA CATCTTCCGG 
CACTTGCCCA AGCTGCAGCA CATCCACATC ACGGGCGGAT CGGGTCTGAC CCGACTGGAG 
GCGGGCCTGT TCGATGGCCT GATCTCACTG AAGAACCTGG ACCTCTCGCA CAACGGACTC 
AACTGGATCC ATCTGCGGGC CCTCACCCGA TTGCCCAATT TGGTCAGCCT GAAGCTGTCC 
CACAATCAGA TCAGCGATGT GGGCATGATC GGTCGCATTG TCAAGGACTT GGAGCACCTG 
AAGAAGCTCA GATTGGATCA CAACATCATC AATGTCATCG AAGACGGGTC GTTTGTAGAT 
CTGCCCAATC TGTCGGAGCT GCATCTGAAC GACAATCGCA TCACCGAGCT GCAGTACGGC 
GCCTTCCTGC GCACGCCCCA GCTGAAGACA ATCTATCTGC AGAACAACCT CATCCGGCGC 
ATACATCCGG AGTCGCTGCT CCAGGCCAGT GGCAGTGGCG TCGAGGCCGT CCACATCTAC 
AACAATGAGA TTGGCCATGT GGAGGCCTTG AGGGCGCTGC TCGACGCCCT GCCCACGCTG 
CGCTACCTCG ACATGAGCGG CAACCTGTTG AGTGATCTGC CCTATGGCGC GCTGCGGGGT 
CACGGCACCT TGGAGCAGCT GCATCTGAAC AACAACCAGC TGAGGCTGAT TGAGCGGGAC 
GCCCTGATGG CCATGCCCGC CCTGCGAGAG CTGCGCATGC GCAACAACAG TCTGTCCTCC 
GATCTGCCGC TGCCGTTCTG GAATCTGCCG GGCCTCAAGG GCCTGGACCT GGCTCAGAAT 
CAGTTTGTGC GAGTCGATTC CCAGCTGCTG GCGGGACTGC CTTCGCTGCG GCGGCTCGAT 
CTCAGCGAGA ACGGACTGAG GGAGCTGGCT CCGAACAGTT TCCGGCACAA TCCATTGCTG 
GAAACCCTCA ATATATCCTC CAATGGCCTG TCAAAGATTC ACTCCTCTAC GTTGCTGCAC 
CTGGAACGGC TGTTCGAGGT CGATGCCAGC TTTAACCAGC TGACAGCGGT GATCGCGGGC 
CTGCCACGCA TTGTGGAACG CATCTCCCTG AAGGGCAACG AGATCGGATC TCTGCCCGCT 
GCGGCCAGCA AGTCCCTGCA GCTGCCCAAC CTGCGCATGC TCGATCTCAG CCAGAACCGC 
ATCGAGCAGC TGCCCAGGCA CGGCTTCGAG GGCGCCGCCC AGTTGCGGGT CTTGAGCCTG 
GCCCAGAACC AGCTGCGCCA GCTGGAGGAC ACCTCCTTTA TAGGCATCCA GCGGCTGGAG 
TTGCTCCATC TGCAGGAGAA CCAGCTGGGG GAGGCCGACG AGCGGGCTCT GTTGCCCCTG 
GCGGAGCTGC GGAATCTCAA CCTCCAGTCG AACAAACTGG AGGCCATCAC CGACAACTTT 
TTCTCGAACA ACAGTCGCCT GGAGCAGCTG GATCTGTCCC GCAACCTGAT ACGCAGCATC 
TCCCCGACGG CCTTCGATAC GCAGAGATCT CTTGAGTATC TCGACCTGTC CGGCAACGGC 
CTGCTGGACA TATCTGTGGG CCTGGGTAAC CTGCACAGCC TTCGGGACAT CGACCTGAGC 
TACAATCAGA TCTCGCGTGT CCAGTCCGAT GTCATTGGGG GCTGGCGCAA CGTCGTCGAG 
ATTCGTTTGT CCAACAATCT GATCGTGGAA CTGCAGCAGG GCACCTTCCG GAACCTGCCG 
AAACTGCAGT ATCTGGATTT GAGCAGCAAT GAGATCAGGA ATGTCGAGCC AGGTGCCCTC 
AAGGGTCTCG ATGAACTGCA GGAGTTCGTC CTCGCAGACA ACAAGCTGGT GGAACTGAAG 
GATCATGTCT TCGAGGAGCT CCCCAGTCTA CTGGCCTCCC ATTTCCAGTA CAACAAGCTG 
CGCTACATCT CCCCGGAGAG CTTCCACAAT GCCAACTCCC TGGTGTTCCT GAACCTGTCC 
AACAATCACT TCAGGAATAT GGAGAACATT GGTCTGCGCA GCATGCGCAA TCTGGAGGTC 
CTGGACTTGT CCACCAATGG GGTCAAACTG GTCTCGACAA TGCCACTGAA GGCACTCAAT 
TGGTTGGTGG AGCTGAAAAT GGATAACAAT CAGATATGTC GGATACAGGG TGCTCCGTTT 
GAGACCATGC CCAGGCTGCG GGTCCTCTCG ATGCGAAACA ATCAGCTGAG AAGCATCAAG 
GAGCGCACCT TCCGGAATCT CAGAGGGAAC ATAGCCATCT TGGATGTGGA TGGCAATCCC 
ATTGACTGCA ACTGTGAGAT GCAGTGGCTT TCGGTTTGGC TGCAGGAAAC AAACTTCCCG 
TATCCCGGGC CCAAGTGCCA GGATGGACGT CTCCTGAGGG CCGCCCGCAT GGAGCGGAGC 
TTGTGTGCGG GCGTGGATGT ATTTAACAAT AATGAACGGA CAGAGGCCAA TCATCTGCCG 
CTGCTCAACG AACACGGCGA TGTCTTCCAG CGGGACCTGC CCGATGACTT CACCGACGAG 
TGTGAAGTGT TCGAGGCCAC GAGGCAGCCC GGCGACCGAC CCATGGTGGG CGAGAGCGAG 
TACTTCTACG ATCAGTATGT GGACTCCACA GATCCGCCGG ACGCGGCCAA CACAGTGCTG 
AGCACCTCGC AGCGCCCGAA GCCCGTTCCC ACGCTCAACA GCAACATCGA CCTGAACAAC 
ACACTGCTCC ACACCAAATA CTTCAACAGA CGTCCACAGC CGGGGGCTGG AGCCGCCGGA 
GGCGGTTCTC CCTTCACCTT CTTTGGCTAT CCCATTCCCA GTGTGAGTCT GGGCCGGTTC 
TTTGGCTTTG GGGATCGCGG CCGCAAGCAG CGAACGGATG GGAACGATGA GATGCCGGCC 
ACGCATCGCA TGGCCAGCAT TAACCTGCCC AGCGGTCGGG GCAAGACGCG GATGTATCAG 
CCCAACAGCG CAGAGTTCGA AAAGTACTTG AAGGAGCAGC AGCAGCAGCA GAATGTGGCC 
AGAAACCGCT ACATGGAAGT GGACACCACC ACCAACTCGG CCGAGGAGAC ACTGAGCAAT 
GAGAGCGGCG GGTCGGCCAC AACAGTCGGA GTTTTTCGCA CAACCTTCCG GGAACCGTCG 
AGCATCGAAC GCGGAGGCTT CCGTCCCATT GTGCCGGCCC ATGTGGGTGG CTTCATGCCC 
GTCCATGACC CGCAGCAGCG ACGCGGTCTG GTGGAGGCTG TTAACGTCAC AGGAAAGCTT 
CCGGAGCCGG CTCAGGCCAA GGCCGAGCGT AACTTTATGC CCATTTCCGT ATATCCTCGC 
CCCAGACCCA CCCCCAATAG TGTGGAGAGT GTCGAATCGG CCACATATGA TGTCACGGAG 
ATGACGCCCG ATGTGACCAC CATAGTGCCG CTTGTCCAAA GCACAAGAGG AGCGACCACG 
CGACCCACAA CCACAACGAG GGCCACAACA ACCACTGTTA GGACTACCAC CACCACCACC 
ACCACCACTC CTGAGCCTAC AATTGTAAAC GATTCCTCCT CGGGCAGCGA GCAGGAGCAA 
CTGGACTCGC ACTACGATGA TGATGATTTG GAGGCGCAGT CGGTGACCTT GCTGCGACCT 
CCGCCCCTGG TGCAGACAAC CACGGCGGAG ACAATTTTGC TGATTCCACC TGCAGAGGAG 
CATGTGGCAC AGATCAAGAG CCACTCCTGG GTGACTACGA CGACTCCACA GAGCCCCAGT 
GACTACCAGG CCACTCCAGT GCGCTCGACA AGCACGGTGC CACCGCCTGC CCCGCCACCT 
CCATTGCGGA GCGGTGGGCG ATCCACCATC ACCAAGGTCT TTACTCCGCA CCAACCACAA 
GGGGCTCAGC CCACAGCGGA GGAATACCAG CGCACCACGC CCAGTGCGGA CAACGAGGGT 
CTGGCCAGCG AGCATCAGCT AACCGCGCAG AAACGCACAG AGCTGGAGCT CTTGCAAGTG 
GATCGGCTAG ACCGCAAGGA TGGCATGGAC TGGTACTACG AGAGCTTCAA GAAGAAGCGC 
GACTTTAATG GCGGGGCTGC AGTGCGAAAA GCAGCCCACA AGGAGGTGTT CTATGGTGGC 
ATCCCCTCGG ATGGGGAAAG TACGGGCAGC TGGAACCGAT TCAACAAGAA GGAGATCATC 
TTTCTCAGCT TTTTATTGTT GTGGAGGCTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001352983.2
CDS	700..5352
Translation	MMILQILLLLCAGTLLTRADSCPPSQAILPCRCSLRGKEIQIWCSHSNLPQIMDGLKAVERNIKSQIDELVLENNQLPALPGRFFGNLQIVRLMLRYNSIERVSNGWLNELENSLVEIFIVEPQLRSIPAESLNGMINMLAITIQSDELKHLPDFSGLLSLTYLSVQTGSLLELAPHIFRHLPKLQHIHITGGSGLTRLEAGLFDGLISLKNLDLSHNGLNWIHLRALTRLPNLVSLKLSHNQISDVGMIGRIVKDLEHLKKLRLDHNIINVIEDGSFVDLPNLSELHLNDNRITELQYGAFLRTPQLKTIYLQNNLIRRIHPESLLQASGSGVEAVHIYNNEIGHVEALRALLDALPTLRYLDMSGNLLSDLPYGALRGHGTLEQLHLNNNQLRLIERDALMAMPALRELRMRNNSLSSDLPLPFWNLPGLKGLDLAQNQFVRVDSQLLAGLPSLRRLDLSENGLRELAPNSFRHNPLLETLNISSNGLSKIHSSTLLHLERLFEVDASFNQLTAVIAGLPRIVERISLKGNEIGSLPAAASKSLQLPNLRMLDLSQNRIEQLPRHGFEGAAQLRVLSLAQNQLRQLEDTSFIGIQRLELLHLQENQLGEADERALLPLAELRNLNLQSNKLEAITDNFFSNNSRLEQLDLSRNLIRSISPTAFDTQRSLEYLDLSGNGLLDISVGLGNLHSLRDIDLSYNQISRVQSDVIGGWRNVVEIRLSNNLIVELQQGTFRNLPKLQYLDLSSNEIRNVEPGALKGLDELQEFVLADNKLVELKDHVFEELPSLLASHFQYNKLRYISPESFHNANSLVFLNLSNNHFRNMENIGLRSMRNLEVLDLSTNGVKLVSTMPLKALNWLVELKMDNNQICRIQGAPFETMPRLRVLSMRNNQLRSIKERTFRNLRGNIAILDVDGNPIDCNCEMQWLSVWLQETNFPYPGPKCQDGRLLRAARMERSLCAGVDVFNNNERTEANHLPLLNEHGDVFQRDLPDDFTDECEVFEATRQPGDRPMVGESEYFYDQYVDSTDPPDAANTVLSTSQRPKPVPTLNSNIDLNNTLLHTKYFNRRPQPGAGAAGGGSPFTFFGYPIPSVSLGRFFGFGDRGRKQRTDGNDEMPATHRMASINLPSGRGKTRMYQPNSAEFEKYLKEQQQQQNVARNRYMEVDTTTNSAEETLSNESGGSATTVGVFRTTFREPSSIERGGFRPIVPAHVGGFMPVHDPQQRRGLVEAVNVTGKLPEPAQAKAERNFMPISVYPRPRPTPNSVESVESATYDVTEMTPDVTTIVPLVQSTRGATTRPTTTTRATTTTVRTTTTTTTTTPEPTIVNDSSSGSEQEQLDSHYDDDDLEAQSVTLLRPPPLVQTTTAETILLIPPAEEHVAQIKSHSWVTTTTPQSPSDYQATPVRSTSTVPPPAPPPPLRSGGRSTITKVFTPHQPQGAQPTAEEYQRTTPSADNEGLASEHQLTAQKRTELELLQVDRLDRKDGMDWYYESFKKKRDFNGGAAVRKAAHKEVFYGGIPSDGESTGSWNRFNKKEIIFLSFLLLWRL

Target ORF information:

RefSeq Version	XM_001352947.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA18727), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001352947.3

ORF Insert Sequence:

ATGATGATAC TCCAGATCTT ACTGCTGCTC TGTGCCGGGA CACTCCTGAC CAGGGCCGAC 
AGCTGTCCGC CCTCTCAGGC CATCCTGCCC TGTCGCTGCT CGCTGCGTGG CAAGGAGATA 
CAGATATGGT GCTCGCACAG CAATCTGCCC CAAATCATGG ACGGGCTGAA GGCCGTGGAG 
CGGAATATCA AGAGCCAAAT CGATGAGCTG GTGCTGGAGA ACAATCAGCT GCCGGCGTTG 
CCGGGACGCT TCTTTGGTAA CCTGCAGATT GTGCGGCTGA TGCTGCGCTA CAACAGCATC 
GAACGGGTGT CCAACGGCTG GCTGAACGAG CTGGAGAACA GCCTGGTGGA GATCTTCATC 
GTGGAGCCGC AGCTGCGGAG CATTCCCGCG GAGAGTCTCA ATGGCATGAT CAATATGCTG 
GCCATAACCA TACAGAGCGA CGAGCTGAAG CATCTGCCGG ACTTCTCGGG CCTGCTCAGC 
CTCACCTACC TGAGTGTGCA GACTGGATCC CTGCTGGAGC TGGCGCCCCA CATCTTCCGG 
CACTTGCCCA AGCTGCAGCA CATCCACATC ACGGGCGGAT CGGGTCTGAC CCGACTGGAG 
GCGGGCCTGT TCGATGGCCT GATCTCACTG AAGAACCTGG ACCTCTCGCA CAACGGACTC 
AACTGGATCC ATCTGCGGGC CCTCACCCGA TTGCCCAATT TGGTCAGCCT GAAGCTGTCC 
CACAATCAGA TCAGCGATGT GGGCATGATC GGTCGCATTG TCAAGGACTT GGAGCACCTG 
AAGAAGCTCA GATTGGATCA CAACATCATC AATGTCATCG AAGACGGGTC GTTTGTAGAT 
CTGCCCAATC TGTCGGAGCT GCATCTGAAC GACAATCGCA TCACCGAGCT GCAGTACGGC 
GCCTTCCTGC GCACGCCCCA GCTGAAGACA ATCTATCTGC AGAACAACCT CATCCGGCGC 
ATACATCCGG AGTCGCTGCT CCAGGCCAGT GGCAGTGGCG TCGAGGCCGT CCACATCTAC 
AACAATGAGA TTGGCCATGT GGAGGCCTTG AGGGCGCTGC TCGACGCCCT GCCCACGCTG 
CGCTACCTCG ACATGAGCGG CAACCTGTTG AGTGATCTGC CCTATGGCGC GCTGCGGGGT 
CACGGCACCT TGGAGCAGCT GCATCTGAAC AACAACCAGC TGAGGCTGAT TGAGCGGGAC 
GCCCTGATGG CCATGCCCGC CCTGCGAGAG CTGCGCATGC GCAACAACAG TCTGTCCTCC 
GATCTGCCGC TGCCGTTCTG GAATCTGCCG GGCCTCAAGG GCCTGGACCT GGCTCAGAAT 
CAGTTTGTGC GAGTCGATTC CCAGCTGCTG GCGGGACTGC CTTCGCTGCG GCGGCTCGAT 
CTCAGCGAGA ACGGACTGAG GGAGCTGGCT CCGAACAGTT TCCGGCACAA TCCATTGCTG 
GAAACCCTCA ATATATCCTC CAATGGCCTG TCAAAGATTC ACTCCTCTAC GTTGCTGCAC 
CTGGAACGGC TGTTCGAGGT CGATGCCAGC TTTAACCAGC TGACAGCGGT GATCGCGGGC 
CTGCCACGCA TTGTGGAACG CATCTCCCTG AAGGGCAACG AGATCGGATC TCTGCCCGCT 
GCGGCCAGCA AGTCCCTGCA GCTGCCCAAC CTGCGCATGC TCGATCTCAG CCAGAACCGC 
ATCGAGCAGC TGCCCAGGCA CGGCTTCGAG GGCGCCGCCC AGTTGCGGGT CTTGAGCCTG 
GCCCAGAACC AGCTGCGCCA GCTGGAGGAC ACCTCCTTTA TAGGCATCCA GCGGCTGGAG 
TTGCTCCATC TGCAGGAGAA CCAGCTGGGG GAGGCCGACG AGCGGGCTCT GTTGCCCCTG 
GCGGAGCTGC GGAATCTCAA CCTCCAGTCG AACAAACTGG AGGCCATCAC CGACAACTTT 
TTCTCGAACA ACAGTCGCCT GGAGCAGCTG GATCTGTCCC GCAACCTGAT ACGCAGCATC 
TCCCCGACGG CCTTCGATAC GCAGAGATCT CTTGAGTATC TCGACCTGTC CGGCAACGGC 
CTGCTGGACA TATCTGTGGG CCTGGGTAAC CTGCACAGCC TTCGGGACAT CGACCTGAGC 
TACAATCAGA TCTCGCGTGT CCAGTCCGAT GTCATTGGGG GCTGGCGCAA CGTCGTCGAG 
ATTCGTTTGT CCAACAATCT GATCGTGGAA CTGCAGCAGG GCACCTTCCG GAACCTGCCG 
AAACTGCAGT ATCTGGATTT GAGCAGCAAT GAGATCAGGA ATGTCGAGCC AGGTGCCCTC 
AAGGGTCTCG ATGAACTGCA GGAGTTCGTC CTCGCAGACA ACAAGCTGGT GGAACTGAAG 
GATCATGTCT TCGAGGAGCT CCCCAGTCTA CTGGCCTCCC ATTTCCAGTA CAACAAGCTG 
CGCTACATCT CCCCGGAGAG CTTCCACAAT GCCAACTCCC TGGTGTTCCT GAACCTGTCC 
AACAATCACT TCAGGAATAT GGAGAACATT GGTCTGCGCA GCATGCGCAA TCTGGAGGTC 
CTGGACTTGT CCACCAATGG GGTCAAACTG GTCTCGACAA TGCCACTGAA GGCACTCAAT 
TGGTTGGTGG AGCTGAAAAT GGATAACAAT CAGATATGTC GGATACAGGG TGCTCCGTTT 
GAGACCATGC CCAGGCTGCG GGTCCTCTCG ATGCGAAACA ATCAGCTGAG AAGCATCAAG 
GAGCGCACCT TCCGGAATCT CAGAGGGAAC ATAGCCATCT TGGATGTGGA TGGCAATCCC 
ATTGACTGCA ACTGTGAGAT GCAGTGGCTT TCGGTTTGGC TGCAGGAAAC AAACTTCCCG 
TATCCCGGGC CCAAGTGCCA GGATGGACGT CTCCTGAGGG CCGCCCGCAT GGAGCGGAGC 
TTGTGTGCGG GCGTGGATGT ATTTAACAAT AATGAACGGA CAGAGGCCAA TCATCTGCCG 
CTGCTCAACG AACACGGCGA TGTCTTCCAG CGGGACCTGC CCGATGACTT CACCGACGAG 
TGTGAAGTGT TCGAGGCCAC GAGGCAGCCC GGCGACCGAC CCATGGTGGG CGAGAGCGAG 
TACTTCTACG ATCAGTATGT GGACTCCACA GATCCGCCGG ACGCGGCCAA CACAGTGCTG 
AGCACCTCGC AGCGCCCGAA GCCCGTTCCC ACGCTCAACA GCAACATCGA CCTGAACAAC 
ACACTGCTCC ACACCAAATA CTTCAACAGA CGTCCACAGC CGGGGGCTGG AGCCGCCGGA 
GGCGGTTCTC CCTTCACCTT CTTTGGCTAT CCCATTCCCA GTGTGAGTCT GGGCCGGTTC 
TTTGGCTTTG GGGATCGCGG CCGCAAGCAG CGAACGGATG GGAACGATGA GATGCCGGCC 
ACGCATCGCA TGGCCAGCAT TAACCTGCCC AGCGGTCGGG GCAAGACGCG GATGTATCAG 
CCCAACAGCG CAGAGTTCGA AAAGTACTTG AAGGAGCAGC AGCAGCAGCA GAATGTGGCC 
AGAAACCGCT ACATGGAAGT GGACACCACC ACCAACTCGG CCGAGGAGAC ACTGAGCAAT 
GAGAGCGGCG GGTCGGCCAC AACAGTCGGA GTTTTTCGCA CAACCTTCCG GGAACCGTCG 
AGCATCGAAC GCGGAGGCTT CCGTCCCATT GTGCCGGCCC ATGTGGGTGG CTTCATGCCC 
GTCCATGACC CGCAGCAGCG ACGCGGTCTG GTGGAGGCTG TTAACGTCAC AGGAAAGCTT 
CCGGAGCCGG CTCAGGCCAA GGCCGAGCGT AACTTTATGC CCATTTCCGT ATATCCTCGC 
CCCAGACCCA CCCCCAATAG TGTGGAGAGT GTCGAATCGG CCACATATGA TGTCACGGAG 
ATGACGCCCG ATGTGACCAC CATAGTGCCG CTTGTCCAAA GCACAAGAGG AGCGACCACG 
CGACCCACAA CCACAACGAG GGCCACAACA ACCACTGTTA GGACTACCAC CACCACCACC 
ACCACCACTC CTGAGCCTAC AATTGTAAAC GATTCCTCCT CGGGCAGCGA GCAGGAGCAA 
CTGGACTCGC ACTACGATGA TGATGATTTG GAGGCGCAGT CGGTGACCTT GCTGCGACCT 
CCGCCCCTGG TGCAGACAAC CACGGCGGAG ACAATTTTGC TGATTCCACC TGCAGAGGAG 
CATGTGGCAC AGATCAAGAG CCACTCCTGG GTGACTACGA CGACTCCACA GAGCCCCAGT 
GACTACCAGG CCACTCCAGT GCGCTCGACA AGCACGGTGC CACCGCCTGC CCCGCCACCT 
CCATTGCGGA GCGGTGGGCG ATCCACCATC ACCAAGGTCT TTACTCCGCA CCAACCACAA 
GGGGCTCAGC CCACAGCGGA GGAATACCAG CGCACCACGC CCAGTGCGGA CAACGAGGGT 
CTGGCCAGCG AGCATCAGCT AACCGCGCAG AAACGCACAG AGCTGGAGCT CTTGCAAGTG 
GATCGGCTAG ACCGCAAGGA TGGCATGGAC TGGTACTACG AGAGCTTCAA GAAGAAGCGC 
GACTTTAATG GCGGGGCTGC AGTGCGAAAA GCAGCCCACA AGGAGGTGTT CTATGGTGGC 
ATCCCCTCGG ATGGGGAAAG TACGGGCAGC TGGAACCGAT TCAACAAGAA GGAGATCATC 
TTTCTCAGCT TTTTATTGTT GTGGAGGCTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA18727 ( XM_015188578.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015044064.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA18727), mRNA.

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CloneID	ODe07260
Clone ID Related Accession (Same CDS sequence)	XM_001352947.3 , XM_015188578.1
Accession Version	XM_015188578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589954). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGATAC TCCAGATCTT ACTGCTGCTC TGTGCCGGGA CACTCCTGAC CAGGGCCGAC 
AGCTGTCCGC CCTCTCAGGC CATCCTGCCC TGTCGCTGCT CGCTGCGTGG CAAGGAGATA 
CAGATATGGT GCTCGCACAG CAATCTGCCC CAAATCATGG ACGGGCTGAA GGCCGTGGAG 
CGGAATATCA AGAGCCAAAT CGATGAGCTG GTGCTGGAGA ACAATCAGCT GCCGGCGTTG 
CCGGGACGCT TCTTTGGTAA CCTGCAGATT GTGCGGCTGA TGCTGCGCTA CAACAGCATC 
GAACGGGTGT CCAACGGCTG GCTGAACGAG CTGGAGAACA GCCTGGTGGA GATCTTCATC 
GTGGAGCCGC AGCTGCGGAG CATTCCCGCG GAGAGTCTCA ATGGCATGAT CAATATGCTG 
GCCATAACCA TACAGAGCGA CGAGCTGAAG CATCTGCCGG ACTTCTCGGG CCTGCTCAGC 
CTCACCTACC TGAGTGTGCA GACTGGATCC CTGCTGGAGC TGGCGCCCCA CATCTTCCGG 
CACTTGCCCA AGCTGCAGCA CATCCACATC ACGGGCGGAT CGGGTCTGAC CCGACTGGAG 
GCGGGCCTGT TCGATGGCCT GATCTCACTG AAGAACCTGG ACCTCTCGCA CAACGGACTC 
AACTGGATCC ATCTGCGGGC CCTCACCCGA TTGCCCAATT TGGTCAGCCT GAAGCTGTCC 
CACAATCAGA TCAGCGATGT GGGCATGATC GGTCGCATTG TCAAGGACTT GGAGCACCTG 
AAGAAGCTCA GATTGGATCA CAACATCATC AATGTCATCG AAGACGGGTC GTTTGTAGAT 
CTGCCCAATC TGTCGGAGCT GCATCTGAAC GACAATCGCA TCACCGAGCT GCAGTACGGC 
GCCTTCCTGC GCACGCCCCA GCTGAAGACA ATCTATCTGC AGAACAACCT CATCCGGCGC 
ATACATCCGG AGTCGCTGCT CCAGGCCAGT GGCAGTGGCG TCGAGGCCGT CCACATCTAC 
AACAATGAGA TTGGCCATGT GGAGGCCTTG AGGGCGCTGC TCGACGCCCT GCCCACGCTG 
CGCTACCTCG ACATGAGCGG CAACCTGTTG AGTGATCTGC CCTATGGCGC GCTGCGGGGT 
CACGGCACCT TGGAGCAGCT GCATCTGAAC AACAACCAGC TGAGGCTGAT TGAGCGGGAC 
GCCCTGATGG CCATGCCCGC CCTGCGAGAG CTGCGCATGC GCAACAACAG TCTGTCCTCC 
GATCTGCCGC TGCCGTTCTG GAATCTGCCG GGCCTCAAGG GCCTGGACCT GGCTCAGAAT 
CAGTTTGTGC GAGTCGATTC CCAGCTGCTG GCGGGACTGC CTTCGCTGCG GCGGCTCGAT 
CTCAGCGAGA ACGGACTGAG GGAGCTGGCT CCGAACAGTT TCCGGCACAA TCCATTGCTG 
GAAACCCTCA ATATATCCTC CAATGGCCTG TCAAAGATTC ACTCCTCTAC GTTGCTGCAC 
CTGGAACGGC TGTTCGAGGT CGATGCCAGC TTTAACCAGC TGACAGCGGT GATCGCGGGC 
CTGCCACGCA TTGTGGAACG CATCTCCCTG AAGGGCAACG AGATCGGATC TCTGCCCGCT 
GCGGCCAGCA AGTCCCTGCA GCTGCCCAAC CTGCGCATGC TCGATCTCAG CCAGAACCGC 
ATCGAGCAGC TGCCCAGGCA CGGCTTCGAG GGCGCCGCCC AGTTGCGGGT CTTGAGCCTG 
GCCCAGAACC AGCTGCGCCA GCTGGAGGAC ACCTCCTTTA TAGGCATCCA GCGGCTGGAG 
TTGCTCCATC TGCAGGAGAA CCAGCTGGGG GAGGCCGACG AGCGGGCTCT GTTGCCCCTG 
GCGGAGCTGC GGAATCTCAA CCTCCAGTCG AACAAACTGG AGGCCATCAC CGACAACTTT 
TTCTCGAACA ACAGTCGCCT GGAGCAGCTG GATCTGTCCC GCAACCTGAT ACGCAGCATC 
TCCCCGACGG CCTTCGATAC GCAGAGATCT CTTGAGTATC TCGACCTGTC CGGCAACGGC 
CTGCTGGACA TATCTGTGGG CCTGGGTAAC CTGCACAGCC TTCGGGACAT CGACCTGAGC 
TACAATCAGA TCTCGCGTGT CCAGTCCGAT GTCATTGGGG GCTGGCGCAA CGTCGTCGAG 
ATTCGTTTGT CCAACAATCT GATCGTGGAA CTGCAGCAGG GCACCTTCCG GAACCTGCCG 
AAACTGCAGT ATCTGGATTT GAGCAGCAAT GAGATCAGGA ATGTCGAGCC AGGTGCCCTC 
AAGGGTCTCG ATGAACTGCA GGAGTTCGTC CTCGCAGACA ACAAGCTGGT GGAACTGAAG 
GATCATGTCT TCGAGGAGCT CCCCAGTCTA CTGGCCTCCC ATTTCCAGTA CAACAAGCTG 
CGCTACATCT CCCCGGAGAG CTTCCACAAT GCCAACTCCC TGGTGTTCCT GAACCTGTCC 
AACAATCACT TCAGGAATAT GGAGAACATT GGTCTGCGCA GCATGCGCAA TCTGGAGGTC 
CTGGACTTGT CCACCAATGG GGTCAAACTG GTCTCGACAA TGCCACTGAA GGCACTCAAT 
TGGTTGGTGG AGCTGAAAAT GGATAACAAT CAGATATGTC GGATACAGGG TGCTCCGTTT 
GAGACCATGC CCAGGCTGCG GGTCCTCTCG ATGCGAAACA ATCAGCTGAG AAGCATCAAG 
GAGCGCACCT TCCGGAATCT CAGAGGGAAC ATAGCCATCT TGGATGTGGA TGGCAATCCC 
ATTGACTGCA ACTGTGAGAT GCAGTGGCTT TCGGTTTGGC TGCAGGAAAC AAACTTCCCG 
TATCCCGGGC CCAAGTGCCA GGATGGACGT CTCCTGAGGG CCGCCCGCAT GGAGCGGAGC 
TTGTGTGCGG GCGTGGATGT ATTTAACAAT AATGAACGGA CAGAGGCCAA TCATCTGCCG 
CTGCTCAACG AACACGGCGA TGTCTTCCAG CGGGACCTGC CCGATGACTT CACCGACGAG 
TGTGAAGTGT TCGAGGCCAC GAGGCAGCCC GGCGACCGAC CCATGGTGGG CGAGAGCGAG 
TACTTCTACG ATCAGTATGT GGACTCCACA GATCCGCCGG ACGCGGCCAA CACAGTGCTG 
AGCACCTCGC AGCGCCCGAA GCCCGTTCCC ACGCTCAACA GCAACATCGA CCTGAACAAC 
ACACTGCTCC ACACCAAATA CTTCAACAGA CGTCCACAGC CGGGGGCTGG AGCCGCCGGA 
GGCGGTTCTC CCTTCACCTT CTTTGGCTAT CCCATTCCCA GTGTGAGTCT GGGCCGGTTC 
TTTGGCTTTG GGGATCGCGG CCGCAAGCAG CGAACGGATG GGAACGATGA GATGCCGGCC 
ACGCATCGCA TGGCCAGCAT TAACCTGCCC AGCGGTCGGG GCAAGACGCG GATGTATCAG 
CCCAACAGCG CAGAGTTCGA AAAGTACTTG AAGGAGCAGC AGCAGCAGCA GAATGTGGCC 
AGAAACCGCT ACATGGAAGT GGACACCACC ACCAACTCGG CCGAGGAGAC ACTGAGCAAT 
GAGAGCGGCG GGTCGGCCAC AACAGTCGGA GTTTTTCGCA CAACCTTCCG GGAACCGTCG 
AGCATCGAAC GCGGAGGCTT CCGTCCCATT GTGCCGGCCC ATGTGGGTGG CTTCATGCCC 
GTCCATGACC CGCAGCAGCG ACGCGGTCTG GTGGAGGCTG TTAACGTCAC AGGAAAGCTT 
CCGGAGCCGG CTCAGGCCAA GGCCGAGCGT AACTTTATGC CCATTTCCGT ATATCCTCGC 
CCCAGACCCA CCCCCAATAG TGTGGAGAGT GTCGAATCGG CCACATATGA TGTCACGGAG 
ATGACGCCCG ATGTGACCAC CATAGTGCCG CTTGTCCAAA GCACAAGAGG AGCGACCACG 
CGACCCACAA CCACAACGAG GGCCACAACA ACCACTGTTA GGACTACCAC CACCACCACC 
ACCACCACTC CTGAGCCTAC AATTGTAAAC GATTCCTCCT CGGGCAGCGA GCAGGAGCAA 
CTGGACTCGC ACTACGATGA TGATGATTTG GAGGCGCAGT CGGTGACCTT GCTGCGACCT 
CCGCCCCTGG TGCAGACAAC CACGGCGGAG ACAATTTTGC TGATTCCACC TGCAGAGGAG 
CATGTGGCAC AGATCAAGAG CCACTCCTGG GTGACTACGA CGACTCCACA GAGCCCCAGT 
GACTACCAGG CCACTCCAGT GCGCTCGACA AGCACGGTGC CACCGCCTGC CCCGCCACCT 
CCATTGCGGA GCGGTGGGCG ATCCACCATC ACCAAGGTCT TTACTCCGCA CCAACCACAA 
GGGGCTCAGC CCACAGCGGA GGAATACCAG CGCACCACGC CCAGTGCGGA CAACGAGGGT 
CTGGCCAGCG AGCATCAGCT AACCGCGCAG AAACGCACAG AGCTGGAGCT CTTGCAAGTG 
GATCGGCTAG ACCGCAAGGA TGGCATGGAC TGGTACTACG AGAGCTTCAA GAAGAAGCGC 
GACTTTAATG GCGGGGCTGC AGTGCGAAAA GCAGCCCACA AGGAGGTGTT CTATGGTGGC 
ATCCCCTCGG ATGGGGAAAG TACGGGCAGC TGGAACCGAT TCAACAAGAA GGAGATCATC 
TTTCTCAGCT TTTTATTGTT GTGGAGGCTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015044064.1
CDS	173..4825
Translation	MMILQILLLLCAGTLLTRADSCPPSQAILPCRCSLRGKEIQIWCSHSNLPQIMDGLKAVERNIKSQIDELVLENNQLPALPGRFFGNLQIVRLMLRYNSIERVSNGWLNELENSLVEIFIVEPQLRSIPAESLNGMINMLAITIQSDELKHLPDFSGLLSLTYLSVQTGSLLELAPHIFRHLPKLQHIHITGGSGLTRLEAGLFDGLISLKNLDLSHNGLNWIHLRALTRLPNLVSLKLSHNQISDVGMIGRIVKDLEHLKKLRLDHNIINVIEDGSFVDLPNLSELHLNDNRITELQYGAFLRTPQLKTIYLQNNLIRRIHPESLLQASGSGVEAVHIYNNEIGHVEALRALLDALPTLRYLDMSGNLLSDLPYGALRGHGTLEQLHLNNNQLRLIERDALMAMPALRELRMRNNSLSSDLPLPFWNLPGLKGLDLAQNQFVRVDSQLLAGLPSLRRLDLSENGLRELAPNSFRHNPLLETLNISSNGLSKIHSSTLLHLERLFEVDASFNQLTAVIAGLPRIVERISLKGNEIGSLPAAASKSLQLPNLRMLDLSQNRIEQLPRHGFEGAAQLRVLSLAQNQLRQLEDTSFIGIQRLELLHLQENQLGEADERALLPLAELRNLNLQSNKLEAITDNFFSNNSRLEQLDLSRNLIRSISPTAFDTQRSLEYLDLSGNGLLDISVGLGNLHSLRDIDLSYNQISRVQSDVIGGWRNVVEIRLSNNLIVELQQGTFRNLPKLQYLDLSSNEIRNVEPGALKGLDELQEFVLADNKLVELKDHVFEELPSLLASHFQYNKLRYISPESFHNANSLVFLNLSNNHFRNMENIGLRSMRNLEVLDLSTNGVKLVSTMPLKALNWLVELKMDNNQICRIQGAPFETMPRLRVLSMRNNQLRSIKERTFRNLRGNIAILDVDGNPIDCNCEMQWLSVWLQETNFPYPGPKCQDGRLLRAARMERSLCAGVDVFNNNERTEANHLPLLNEHGDVFQRDLPDDFTDECEVFEATRQPGDRPMVGESEYFYDQYVDSTDPPDAANTVLSTSQRPKPVPTLNSNIDLNNTLLHTKYFNRRPQPGAGAAGGGSPFTFFGYPIPSVSLGRFFGFGDRGRKQRTDGNDEMPATHRMASINLPSGRGKTRMYQPNSAEFEKYLKEQQQQQNVARNRYMEVDTTTNSAEETLSNESGGSATTVGVFRTTFREPSSIERGGFRPIVPAHVGGFMPVHDPQQRRGLVEAVNVTGKLPEPAQAKAERNFMPISVYPRPRPTPNSVESVESATYDVTEMTPDVTTIVPLVQSTRGATTRPTTTTRATTTTVRTTTTTTTTTPEPTIVNDSSSGSEQEQLDSHYDDDDLEAQSVTLLRPPPLVQTTTAETILLIPPAEEHVAQIKSHSWVTTTTPQSPSDYQATPVRSTSTVPPPAPPPPLRSGGRSTITKVFTPHQPQGAQPTAEEYQRTTPSADNEGLASEHQLTAQKRTELELLQVDRLDRKDGMDWYYESFKKKRDFNGGAAVRKAAHKEVFYGGIPSDGESTGSWNRFNKKEIIFLSFLLLWRL

Target ORF information:

RefSeq Version	XM_015188578.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA18727), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015188578.1

ORF Insert Sequence:

ATGATGATAC TCCAGATCTT ACTGCTGCTC TGTGCCGGGA CACTCCTGAC CAGGGCCGAC 
AGCTGTCCGC CCTCTCAGGC CATCCTGCCC TGTCGCTGCT CGCTGCGTGG CAAGGAGATA 
CAGATATGGT GCTCGCACAG CAATCTGCCC CAAATCATGG ACGGGCTGAA GGCCGTGGAG 
CGGAATATCA AGAGCCAAAT CGATGAGCTG GTGCTGGAGA ACAATCAGCT GCCGGCGTTG 
CCGGGACGCT TCTTTGGTAA CCTGCAGATT GTGCGGCTGA TGCTGCGCTA CAACAGCATC 
GAACGGGTGT CCAACGGCTG GCTGAACGAG CTGGAGAACA GCCTGGTGGA GATCTTCATC 
GTGGAGCCGC AGCTGCGGAG CATTCCCGCG GAGAGTCTCA ATGGCATGAT CAATATGCTG 
GCCATAACCA TACAGAGCGA CGAGCTGAAG CATCTGCCGG ACTTCTCGGG CCTGCTCAGC 
CTCACCTACC TGAGTGTGCA GACTGGATCC CTGCTGGAGC TGGCGCCCCA CATCTTCCGG 
CACTTGCCCA AGCTGCAGCA CATCCACATC ACGGGCGGAT CGGGTCTGAC CCGACTGGAG 
GCGGGCCTGT TCGATGGCCT GATCTCACTG AAGAACCTGG ACCTCTCGCA CAACGGACTC 
AACTGGATCC ATCTGCGGGC CCTCACCCGA TTGCCCAATT TGGTCAGCCT GAAGCTGTCC 
CACAATCAGA TCAGCGATGT GGGCATGATC GGTCGCATTG TCAAGGACTT GGAGCACCTG 
AAGAAGCTCA GATTGGATCA CAACATCATC AATGTCATCG AAGACGGGTC GTTTGTAGAT 
CTGCCCAATC TGTCGGAGCT GCATCTGAAC GACAATCGCA TCACCGAGCT GCAGTACGGC 
GCCTTCCTGC GCACGCCCCA GCTGAAGACA ATCTATCTGC AGAACAACCT CATCCGGCGC 
ATACATCCGG AGTCGCTGCT CCAGGCCAGT GGCAGTGGCG TCGAGGCCGT CCACATCTAC 
AACAATGAGA TTGGCCATGT GGAGGCCTTG AGGGCGCTGC TCGACGCCCT GCCCACGCTG 
CGCTACCTCG ACATGAGCGG CAACCTGTTG AGTGATCTGC CCTATGGCGC GCTGCGGGGT 
CACGGCACCT TGGAGCAGCT GCATCTGAAC AACAACCAGC TGAGGCTGAT TGAGCGGGAC 
GCCCTGATGG CCATGCCCGC CCTGCGAGAG CTGCGCATGC GCAACAACAG TCTGTCCTCC 
GATCTGCCGC TGCCGTTCTG GAATCTGCCG GGCCTCAAGG GCCTGGACCT GGCTCAGAAT 
CAGTTTGTGC GAGTCGATTC CCAGCTGCTG GCGGGACTGC CTTCGCTGCG GCGGCTCGAT 
CTCAGCGAGA ACGGACTGAG GGAGCTGGCT CCGAACAGTT TCCGGCACAA TCCATTGCTG 
GAAACCCTCA ATATATCCTC CAATGGCCTG TCAAAGATTC ACTCCTCTAC GTTGCTGCAC 
CTGGAACGGC TGTTCGAGGT CGATGCCAGC TTTAACCAGC TGACAGCGGT GATCGCGGGC 
CTGCCACGCA TTGTGGAACG CATCTCCCTG AAGGGCAACG AGATCGGATC TCTGCCCGCT 
GCGGCCAGCA AGTCCCTGCA GCTGCCCAAC CTGCGCATGC TCGATCTCAG CCAGAACCGC 
ATCGAGCAGC TGCCCAGGCA CGGCTTCGAG GGCGCCGCCC AGTTGCGGGT CTTGAGCCTG 
GCCCAGAACC AGCTGCGCCA GCTGGAGGAC ACCTCCTTTA TAGGCATCCA GCGGCTGGAG 
TTGCTCCATC TGCAGGAGAA CCAGCTGGGG GAGGCCGACG AGCGGGCTCT GTTGCCCCTG 
GCGGAGCTGC GGAATCTCAA CCTCCAGTCG AACAAACTGG AGGCCATCAC CGACAACTTT 
TTCTCGAACA ACAGTCGCCT GGAGCAGCTG GATCTGTCCC GCAACCTGAT ACGCAGCATC 
TCCCCGACGG CCTTCGATAC GCAGAGATCT CTTGAGTATC TCGACCTGTC CGGCAACGGC 
CTGCTGGACA TATCTGTGGG CCTGGGTAAC CTGCACAGCC TTCGGGACAT CGACCTGAGC 
TACAATCAGA TCTCGCGTGT CCAGTCCGAT GTCATTGGGG GCTGGCGCAA CGTCGTCGAG 
ATTCGTTTGT CCAACAATCT GATCGTGGAA CTGCAGCAGG GCACCTTCCG GAACCTGCCG 
AAACTGCAGT ATCTGGATTT GAGCAGCAAT GAGATCAGGA ATGTCGAGCC AGGTGCCCTC 
AAGGGTCTCG ATGAACTGCA GGAGTTCGTC CTCGCAGACA ACAAGCTGGT GGAACTGAAG 
GATCATGTCT TCGAGGAGCT CCCCAGTCTA CTGGCCTCCC ATTTCCAGTA CAACAAGCTG 
CGCTACATCT CCCCGGAGAG CTTCCACAAT GCCAACTCCC TGGTGTTCCT GAACCTGTCC 
AACAATCACT TCAGGAATAT GGAGAACATT GGTCTGCGCA GCATGCGCAA TCTGGAGGTC 
CTGGACTTGT CCACCAATGG GGTCAAACTG GTCTCGACAA TGCCACTGAA GGCACTCAAT 
TGGTTGGTGG AGCTGAAAAT GGATAACAAT CAGATATGTC GGATACAGGG TGCTCCGTTT 
GAGACCATGC CCAGGCTGCG GGTCCTCTCG ATGCGAAACA ATCAGCTGAG AAGCATCAAG 
GAGCGCACCT TCCGGAATCT CAGAGGGAAC ATAGCCATCT TGGATGTGGA TGGCAATCCC 
ATTGACTGCA ACTGTGAGAT GCAGTGGCTT TCGGTTTGGC TGCAGGAAAC AAACTTCCCG 
TATCCCGGGC CCAAGTGCCA GGATGGACGT CTCCTGAGGG CCGCCCGCAT GGAGCGGAGC 
TTGTGTGCGG GCGTGGATGT ATTTAACAAT AATGAACGGA CAGAGGCCAA TCATCTGCCG 
CTGCTCAACG AACACGGCGA TGTCTTCCAG CGGGACCTGC CCGATGACTT CACCGACGAG 
TGTGAAGTGT TCGAGGCCAC GAGGCAGCCC GGCGACCGAC CCATGGTGGG CGAGAGCGAG 
TACTTCTACG ATCAGTATGT GGACTCCACA GATCCGCCGG ACGCGGCCAA CACAGTGCTG 
AGCACCTCGC AGCGCCCGAA GCCCGTTCCC ACGCTCAACA GCAACATCGA CCTGAACAAC 
ACACTGCTCC ACACCAAATA CTTCAACAGA CGTCCACAGC CGGGGGCTGG AGCCGCCGGA 
GGCGGTTCTC CCTTCACCTT CTTTGGCTAT CCCATTCCCA GTGTGAGTCT GGGCCGGTTC 
TTTGGCTTTG GGGATCGCGG CCGCAAGCAG CGAACGGATG GGAACGATGA GATGCCGGCC 
ACGCATCGCA TGGCCAGCAT TAACCTGCCC AGCGGTCGGG GCAAGACGCG GATGTATCAG 
CCCAACAGCG CAGAGTTCGA AAAGTACTTG AAGGAGCAGC AGCAGCAGCA GAATGTGGCC 
AGAAACCGCT ACATGGAAGT GGACACCACC ACCAACTCGG CCGAGGAGAC ACTGAGCAAT 
GAGAGCGGCG GGTCGGCCAC AACAGTCGGA GTTTTTCGCA CAACCTTCCG GGAACCGTCG 
AGCATCGAAC GCGGAGGCTT CCGTCCCATT GTGCCGGCCC ATGTGGGTGG CTTCATGCCC 
GTCCATGACC CGCAGCAGCG ACGCGGTCTG GTGGAGGCTG TTAACGTCAC AGGAAAGCTT 
CCGGAGCCGG CTCAGGCCAA GGCCGAGCGT AACTTTATGC CCATTTCCGT ATATCCTCGC 
CCCAGACCCA CCCCCAATAG TGTGGAGAGT GTCGAATCGG CCACATATGA TGTCACGGAG 
ATGACGCCCG ATGTGACCAC CATAGTGCCG CTTGTCCAAA GCACAAGAGG AGCGACCACG 
CGACCCACAA CCACAACGAG GGCCACAACA ACCACTGTTA GGACTACCAC CACCACCACC 
ACCACCACTC CTGAGCCTAC AATTGTAAAC GATTCCTCCT CGGGCAGCGA GCAGGAGCAA 
CTGGACTCGC ACTACGATGA TGATGATTTG GAGGCGCAGT CGGTGACCTT GCTGCGACCT 
CCGCCCCTGG TGCAGACAAC CACGGCGGAG ACAATTTTGC TGATTCCACC TGCAGAGGAG 
CATGTGGCAC AGATCAAGAG CCACTCCTGG GTGACTACGA CGACTCCACA GAGCCCCAGT 
GACTACCAGG CCACTCCAGT GCGCTCGACA AGCACGGTGC CACCGCCTGC CCCGCCACCT 
CCATTGCGGA GCGGTGGGCG ATCCACCATC ACCAAGGTCT TTACTCCGCA CCAACCACAA 
GGGGCTCAGC CCACAGCGGA GGAATACCAG CGCACCACGC CCAGTGCGGA CAACGAGGGT 
CTGGCCAGCG AGCATCAGCT AACCGCGCAG AAACGCACAG AGCTGGAGCT CTTGCAAGTG 
GATCGGCTAG ACCGCAAGGA TGGCATGGAC TGGTACTACG AGAGCTTCAA GAAGAAGCGC 
GACTTTAATG GCGGGGCTGC AGTGCGAAAA GCAGCCCACA AGGAGGTGTT CTATGGTGGC 
ATCCCCTCGG ATGGGGAAAG TACGGGCAGC TGGAACCGAT TCAACAAGAA GGAGATCATC 
TTTCTCAGCT TTTTATTGTT GTGGAGGCTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA18727 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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