Dpse\GA17709 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA17709
Entrez Gene ID	4805560
Full Name	GA17709 gene product from transcript GA17709-RB
Synonyms	GA17709,dpse_GLEANR_16360
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_015185236.1	XP_015040722.1	uncharacterized protein, isoform B
XM_001361908.4	XP_001361945.2	uncharacterized protein, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA17709 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA17709 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe04656	XM_001361908.4 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA17709), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODe23557	XM_015185236.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA17709), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA17709 ( XM_001361908.4 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001361945.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA17709), mRNA.

Price & Availability↑

CloneID	ODe04656
Clone ID Related Accession (Same CDS sequence)	XM_001361908.4
Accession Version	XM_001361908.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). On Dec 22, 2015 this sequence version replaced XM_001361908.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCTAA GTGCTCCTGC CTGTAAGGAT ACACAGGACT TTCAATTTAA AGTGCATAAC 
AAGTATGCGG CGTTCAAAAA ATGCGGCGAG CCCCGCAGCA ATGTAAATCT TCTGGCGATT 
TCCTGCAGCC GCGGCTTGCT CTTTGCCGGC AACCCGACGC AGCCGGAACT TAAGGTGGTC 
ATCCTCAAGG ATTTGGCCAA TTCCAACAAG TCGCTCGGCG AGCAGCCGCA GGCGAGGAGT 
GTACCCCTGC CCAGCGTGCC CAACTATATC TCCTGCAGTG CCGATGGCAC TCTTCTGGCG 
GTTAACCTCA CACAGAATGG CACTGGCCTG CTCAACATTT ACTCGGTGCC GACATTCTTG 
GCCGCAGATG TGAAGCTCGT CTACAGCATT CGCCTGGCCA GCGAGGACTA TATCCATGCC 
GTGCAGCTGC TGTGGAATCC GGTCTTGCCC GGAAGTCTGG CCGTAGTGCT GAGCAACGGT 
GCTCTCGGCA TGTACACCCT GAAGGAGGGG GACACATTCG AGATGCATTC GCTGGACAAG 
AGTCAGCAAG TGAAGTGTGG CTGTTGGTCA CCCAAGGGAA AGCAAATTGT CCTAGGTTTT 
CCCGGTGGCA AGATACAGCA GTTCAAGCCG GATCTTACGC CAGCAAAGAC ACTGGTGTGT 
CCACCCAACA TCCACGATGC TCCGTTCGAC ACCATCGCCA TTCAGTGGCT CTCGACGTAC 
CAGTTCGCAG TGGTCTTCCT GCAGCATGGC GAGGACTGCA GTCCCGCCCT GTACATATTG 
AATGCCCCCA AGGGGGGATC GCCCAGCTAT ACCAACTACT ACGACATCTG CTACAGTCTC 
AACGGGCCAC GGAATCATCA GTTTTTCTTC AACCATGTGG CCCAGTGGAA TTTGCTTCTG 
GTGACATCGG CCAATGGCGT GGAAGTGGGT ATAATGGGTA CCTTCGAGTC TGGAGACACC 
CCCGTCTGGC AGCAGTTTAC CCTTCTGGAT GAGGCGAGGA TCGAGCTGCC TCTGAGCGAG 
GGCACCCAAG AGGAAACCTT CCCCCTAGGG TCTGTCTACG ACACATCCTC CACGCACCAA 
CTGACAATCA ACGAGCAGAA GCTCCCCACT ATGCCCATGG TGCATGTCTT AGGCACCGAC 
GGCACCCTTC TATCCTTCAA CTTCCTGAAT CTACTCCAAG GTGCCCCCTC TGTGTGCTCT 
CCGCCTCCGC CGGTGTTTGA CAGTTCGGGA CAGTTCCGCC CCCTGGACAC GTTACTGCCA 
GCGGAGGAAA AGGAGGCGGC CACAACACCG CCAGAAAAGG AGTCGACTCA GTCCGTTCCC 
ACGGACATGT CCTTTGCCTT CTCCCCGAAC ACGGTGACAT CGACTCCAGC TCCTGCCAAG 
GACAAGCCTT CCTCGCTGTT CTCCGGCTTC GCTGCACCCG CAGCAAGTAC TCCATTTGGA 
GTTGCTCCAA CTGCTGCTCC CACCGCGACA GTAGCGAGTA CCGCCTCGGC ATTCGGTGGG 
TTTGGTGCGG CCACGACGAC TCCAGCCTTA GGGTCCATAT TTTCCGCGCC AGGAGCGACC 
GCTTTTGGTG GTCTGGCTTT GGGTAAATCC AACGCAATGC CAGTGAGTGT GGCCAGTGCT 
CCTCTCGCCG TGCCACCGTC TGCAGCTGCT GCGCCCTTAA ACAAGCCCCT CTACACAGTT 
CCGCACACAT TTACGCCAGT GGCAACAAAG CAGCAACCGG CAGCGGCTCC CGTGTCCGTT 
CCATCAAGAC AGTCGCTGGA TAACACGGAG TCCATCGTTA GAGACATGAT AGCGCTACAG 
ATCGAAGCCT TCAACCAGGA CATACAGCAG CAGAAGGGAC TCACCACGGA GCTGCTCAGC 
ACCATTGCAG CTCCCTCGGC TCTCAAAAAG TATGCCAAGC GCTTGGACGA CCTCGAGGAG 
CTGAACGAGC AGGCCAAGGA CGTGGAGTTC GAGTTGGATG TGCAAGGACT GCGCCAGGGT 
CTAAATGAGG CCTACGCCAT AGTGGCCGAG TGCCGTGGCA AGCTGGAGGT CTACGAGAAG 
CCCAACATCA CGCGGCTGAT GAACTCGAGT TCCTATGATC CTGCTGGACG GCGCATGCTG 
GCTCGTCTCC GGAGCTACGT GGCGGCCAAC GAGGCGCAAC TTCGTCTGGC CGAGCAGCAA 
TTGGATCTGC AGTGGGACCA GCTTCAAGAT GTGTTGCGGC GCAAGACCAA GTCGAGCATG 
CATATGCCGT GCCTGGAGGG GATCTACCAG CAACTGACGC GGCTCCAGAA CCTTGCCTCT 
GACCAGAGGA TCATGCAGAA CAGCATCAAG GCCAAGCTCA AGGAGCGTGG CCTGCTCCAG 
GCCGCACTTC TCGACCAGGA CAAGAGTCGC ACTCGCACCA ATGAAACTGT GGAAACCTTG 
GCTGACTCGA TTCTCTCGAT GAGTCTTAGT CAGACTGTCG ATACGAATGC CGCCAAGCTC 
ACACAGCACA AGCTCCGCCA GATCCGAAAC GTGGTGCAGC TGCAAAAGGT CAGCATCATT 
AGTCCCCAGC GTCCAGATCG TGTGGGGCTC AAGTCCGAAG TTATACTCGA GACCAAGCGC 
AAGGCAGAGA TATTGAAAAA GGCCGCCGCA AAGGCGACTA CTGCCAACAA GGCCACCCAG 
GTGGCGGCAC CGGCACCGGC ACCTGCACCT GCACCAACTC CAGCTCCAGC CGCTGCTCCT 
GTTGCGCCCC AACTACCAAA ACCAACAGCA TCCAAGCCCG CGGCCAGCAT CCCGGCCCTC 
AGTGCTGCAC CCATCGCAAC GCCATTCGCC TTCAGCCAGA GCAGTCCGTT TGTGAAGACT 
TCGAGTGTGA CCACGACAAC CACCACGGTA AGCTCTGGAG AGGCTCCAAA GCCGGCTGTC 
TCTTTGTTCG GAGGGCCAAG TAGGTCCGGC TTGAGCTTTG GACCGGTAGG AGGTGGTCTT 
TCGTTTGGGG GACTTTCTGC AGCTGATAAT TCAACTGGGG CCAAGCCGTC CCTGTTTGGA 
GGATTTGGGA TTAGTAAGGA GGCCACAGCA AAGCCGGCTC CTGAGCCAGC AAAGGCACAG 
GATCCCTTGG CGAAGGATAC TACACCTTTT ACTGCAGAAG TGGAGCCAGC GAAGGCACCG 
GAAACCCCAA AAGCAGAGAC TGCTGGCAAA TCCTTTTCCT TCACAGGATT CGGAGGAACT 
GGGGCTCCCA GTTCCACGTT TGGCTTCGGA GGACTGGGCT CTTCCCTTGG TTTTGGCGGC 
GTAGCAAAGA CCGATTCCAA CGCCGCAGTG ACGTCATCAG CTTCTGGCGT TCCCCGTGGC 
TTGTTCGCCT CAGCTGCAGC TGCACCGAAG CCTAACGAAG GTGATACAGC GGTGACCAGC 
ATCAGCACCA CCACCAAGCC AACGGTCGCA CCTGCGTCCA CCTTGTCTGC CGCTGCCGCT 
ACACCGTTGC CAACAAATAA CAGCGGAGTG ACCAGTACCG CCCCCAGCAG CTCTGATTCC 
CTTGGAGGGC TTTTCGGATC TGTCAATATA TGCAAGCCAC AAATCAAGGA ATCCTCCGGC 
GCTTCTGGAC AGCCTGCAAA TATCTTTGGC AGTGGATTCT CATTTGGCGG ACGAGGTGGA 
GATGAACCGA AGGGATTATT TGCGACCATC ACTCCAGCCA CAACAGCAAC AGCAACGGCA 
ACAGAAACAC CTGCAACCGC AGCACCAACT ACGATAAAAA TGGAGCCAGT GAGCACCACA 
GCCGCAACAA CTGCAGCACC TGCACCAGCT GCATCGGCTG CTCCGGCAAC AACCACTTCA 
ACTGAAACTA AGGGGGCTGC AACTCCACCC ACATCCACAC CGGCCGCTGC TCCGCCAGTA 
AAGGTTGCGG TTCCTCCGAA CACGACTTCA GCTGCAGCGC CAGCGGTCAC GCCTGCGACA 
GCAGTCTCTT CGACTGTGAC CTCGAGCACA GCGGTGCCAG GCAGTACATT CTCCTTCTCC 
AATGCATTCA GCAGCCTCAG CACCGCTCCG GCCACCCCCG TCACAGCCAC CAGCTCCAGT 
GCCTCTACAG TGAGCAATTC ATCGTCTTCG GTGTTTGGAG GATCCTTTAC AGCAGCACCA 
GCAACAACAG CGGCACCTGT AGCCAGCAAT CCATTCCAGG CATCGACAAC GGCTTCTGCA 
GCAACCTCCA CGAACCTTTT TGGAAATGTG GCCAAGTCCG AGACTAGCGT ATTTGGAGGT 
TCACCTGGAA CCAATCTCCC TCCTGCTGCA GCTGCTGCCC CACCCAGTGG TCTCTTTGCT 
GCAGCAGCTA TCAGTAATCC GGCGCCTTTT GGCAGCCCTC CACCGGGAAC GGCCGCCTCC 
GGGGGCAACA TCTTTAGCCA GGCCAACAAA CCAAGTGTTT TCGGTCAGCC GTCTGCAGGA 
GCGAGCGGCG GTACGTCTAT TTTTGGAGGG TCATCCGGAT CTCCATTTGG ATCGACCTCC 
ATTTTTGGCG GAAGCAATGC GCAAAGCCCT GCCAATGCAC CCACTGCCGG AAGTACATCC 
ATATTCGGTC AGAACGTCTT TGGGCAACCG GCGGCACAAA CAGCGACCCC AGTGGCAGGC 
GGTAATATAT TTGCAAGTCC TGTGAGCGCC AGCCAACAAC CGCAACCCTC TGCTTTTGGT 
GGAGGCGGAT CTATTTTTGG CGGACCGGCG ACGACTAGTT CTTCGCCTGC TTCTGGTGGA 
TCAATCTTTG GCGGTGGCAG CAGCTCTGGA TTTGGTTCAT TCTCCCAGAC TGCCCCTTCT 
CCTGGAGGCT TTGGCAGTGG CTTTGCTCAG GGCGCCGGAT CGTCAGTGGC CCAATCCGGC 
TTTGGATCAC CCCAAACGCC ACAGCAGGCT GCTCCGGGTG GATTTGGAGC CAAGCCCGTA 
TTCGGTGGTT CTCCGGCCTT CGGGGCCAGT CCCACCTTTG GGGGAGGCGC AACGTTTGGC 
AGTCCCAAGG GATTTGGCGG CTTTGGAGCC AGCCCTAACG TGGCCGCTCC ACCAGCCTTC 
GGAGCCCCTG CGAAACCGGC CGGCGGCAAC ATCTTCGACA CCTTGGGTGG TCAGGACTCC 
GGCTTGTCCT TTGGCAATCT GGCCCAGACC GGCAACCCCA ATGCCCAGAA GCCAGCTTTT 
GGCGGATCGA GTTTTATGAA TTATCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001361945.2
CDS	231..5360
Translation	MALSAPACKDTQDFQFKVHNKYAAFKKCGEPRSNVNLLAISCSRGLLFAGNPTQPELKVVILKDLANSNKSLGEQPQARSVPLPSVPNYISCSADGTLLAVNLTQNGTGLLNIYSVPTFLAADVKLVYSIRLASEDYIHAVQLLWNPVLPGSLAVVLSNGALGMYTLKEGDTFEMHSLDKSQQVKCGCWSPKGKQIVLGFPGGKIQQFKPDLTPAKTLVCPPNIHDAPFDTIAIQWLSTYQFAVVFLQHGEDCSPALYILNAPKGGSPSYTNYYDICYSLNGPRNHQFFFNHVAQWNLLLVTSANGVEVGIMGTFESGDTPVWQQFTLLDEARIELPLSEGTQEETFPLGSVYDTSSTHQLTINEQKLPTMPMVHVLGTDGTLLSFNFLNLLQGAPSVCSPPPPVFDSSGQFRPLDTLLPAEEKEAATTPPEKESTQSVPTDMSFAFSPNTVTSTPAPAKDKPSSLFSGFAAPAASTPFGVAPTAAPTATVASTASAFGGFGAATTTPALGSIFSAPGATAFGGLALGKSNAMPVSVASAPLAVPPSAAAAPLNKPLYTVPHTFTPVATKQQPAAAPVSVPSRQSLDNTESIVRDMIALQIEAFNQDIQQQKGLTTELLSTIAAPSALKKYAKRLDDLEELNEQAKDVEFELDVQGLRQGLNEAYAIVAECRGKLEVYEKPNITRLMNSSSYDPAGRRMLARLRSYVAANEAQLRLAEQQLDLQWDQLQDVLRRKTKSSMHMPCLEGIYQQLTRLQNLASDQRIMQNSIKAKLKERGLLQAALLDQDKSRTRTNETVETLADSILSMSLSQTVDTNAAKLTQHKLRQIRNVVQLQKVSIISPQRPDRVGLKSEVILETKRKAEILKKAAAKATTANKATQVAAPAPAPAPAPTPAPAAAPVAPQLPKPTASKPAASIPALSAAPIATPFAFSQSSPFVKTSSVTTTTTTVSSGEAPKPAVSLFGGPSRSGLSFGPVGGGLSFGGLSAADNSTGAKPSLFGGFGISKEATAKPAPEPAKAQDPLAKDTTPFTAEVEPAKAPETPKAETAGKSFSFTGFGGTGAPSSTFGFGGLGSSLGFGGVAKTDSNAAVTSSASGVPRGLFASAAAAPKPNEGDTAVTSISTTTKPTVAPASTLSAAAATPLPTNNSGVTSTAPSSSDSLGGLFGSVNICKPQIKESSGASGQPANIFGSGFSFGGRGGDEPKGLFATITPATTATATATETPATAAPTTIKMEPVSTTAATTAAPAPAASAAPATTTSTETKGAATPPTSTPAAAPPVKVAVPPNTTSAAAPAVTPATAVSSTVTSSTAVPGSTFSFSNAFSSLSTAPATPVTATSSSASTVSNSSSSVFGGSFTAAPATTAAPVASNPFQASTTASAATSTNLFGNVAKSETSVFGGSPGTNLPPAAAAAPPSGLFAAAAISNPAPFGSPPPGTAASGGNIFSQANKPSVFGQPSAGASGGTSIFGGSSGSPFGSTSIFGGSNAQSPANAPTAGSTSIFGQNVFGQPAAQTATPVAGGNIFASPVSASQQPQPSAFGGGGSIFGGPATTSSSPASGGSIFGGGSSSGFGSFSQTAPSPGGFGSGFAQGAGSSVAQSGFGSPQTPQQAAPGGFGAKPVFGGSPAFGASPTFGGGATFGSPKGFGGFGASPNVAAPPAFGAPAKPAGGNIFDTLGGQDSGLSFGNLAQTGNPNAQKPAFGGSSFMNYR

Target ORF information:

RefSeq Version	XM_001361908.4
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA17709), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001361908.4

ORF Insert Sequence:

ATGGCGCTAA GTGCTCCTGC CTGTAAGGAT ACACAGGACT TTCAATTTAA AGTGCATAAC 
AAGTATGCGG CGTTCAAAAA ATGCGGCGAG CCCCGCAGCA ATGTAAATCT TCTGGCGATT 
TCCTGCAGCC GCGGCTTGCT CTTTGCCGGC AACCCGACGC AGCCGGAACT TAAGGTGGTC 
ATCCTCAAGG ATTTGGCCAA TTCCAACAAG TCGCTCGGCG AGCAGCCGCA GGCGAGGAGT 
GTACCCCTGC CCAGCGTGCC CAACTATATC TCCTGCAGTG CCGATGGCAC TCTTCTGGCG 
GTTAACCTCA CACAGAATGG CACTGGCCTG CTCAACATTT ACTCGGTGCC GACATTCTTG 
GCCGCAGATG TGAAGCTCGT CTACAGCATT CGCCTGGCCA GCGAGGACTA TATCCATGCC 
GTGCAGCTGC TGTGGAATCC GGTCTTGCCC GGAAGTCTGG CCGTAGTGCT GAGCAACGGT 
GCTCTCGGCA TGTACACCCT GAAGGAGGGG GACACATTCG AGATGCATTC GCTGGACAAG 
AGTCAGCAAG TGAAGTGTGG CTGTTGGTCA CCCAAGGGAA AGCAAATTGT CCTAGGTTTT 
CCCGGTGGCA AGATACAGCA GTTCAAGCCG GATCTTACGC CAGCAAAGAC ACTGGTGTGT 
CCACCCAACA TCCACGATGC TCCGTTCGAC ACCATCGCCA TTCAGTGGCT CTCGACGTAC 
CAGTTCGCAG TGGTCTTCCT GCAGCATGGC GAGGACTGCA GTCCCGCCCT GTACATATTG 
AATGCCCCCA AGGGGGGATC GCCCAGCTAT ACCAACTACT ACGACATCTG CTACAGTCTC 
AACGGGCCAC GGAATCATCA GTTTTTCTTC AACCATGTGG CCCAGTGGAA TTTGCTTCTG 
GTGACATCGG CCAATGGCGT GGAAGTGGGT ATAATGGGTA CCTTCGAGTC TGGAGACACC 
CCCGTCTGGC AGCAGTTTAC CCTTCTGGAT GAGGCGAGGA TCGAGCTGCC TCTGAGCGAG 
GGCACCCAAG AGGAAACCTT CCCCCTAGGG TCTGTCTACG ACACATCCTC CACGCACCAA 
CTGACAATCA ACGAGCAGAA GCTCCCCACT ATGCCCATGG TGCATGTCTT AGGCACCGAC 
GGCACCCTTC TATCCTTCAA CTTCCTGAAT CTACTCCAAG GTGCCCCCTC TGTGTGCTCT 
CCGCCTCCGC CGGTGTTTGA CAGTTCGGGA CAGTTCCGCC CCCTGGACAC GTTACTGCCA 
GCGGAGGAAA AGGAGGCGGC CACAACACCG CCAGAAAAGG AGTCGACTCA GTCCGTTCCC 
ACGGACATGT CCTTTGCCTT CTCCCCGAAC ACGGTGACAT CGACTCCAGC TCCTGCCAAG 
GACAAGCCTT CCTCGCTGTT CTCCGGCTTC GCTGCACCCG CAGCAAGTAC TCCATTTGGA 
GTTGCTCCAA CTGCTGCTCC CACCGCGACA GTAGCGAGTA CCGCCTCGGC ATTCGGTGGG 
TTTGGTGCGG CCACGACGAC TCCAGCCTTA GGGTCCATAT TTTCCGCGCC AGGAGCGACC 
GCTTTTGGTG GTCTGGCTTT GGGTAAATCC AACGCAATGC CAGTGAGTGT GGCCAGTGCT 
CCTCTCGCCG TGCCACCGTC TGCAGCTGCT GCGCCCTTAA ACAAGCCCCT CTACACAGTT 
CCGCACACAT TTACGCCAGT GGCAACAAAG CAGCAACCGG CAGCGGCTCC CGTGTCCGTT 
CCATCAAGAC AGTCGCTGGA TAACACGGAG TCCATCGTTA GAGACATGAT AGCGCTACAG 
ATCGAAGCCT TCAACCAGGA CATACAGCAG CAGAAGGGAC TCACCACGGA GCTGCTCAGC 
ACCATTGCAG CTCCCTCGGC TCTCAAAAAG TATGCCAAGC GCTTGGACGA CCTCGAGGAG 
CTGAACGAGC AGGCCAAGGA CGTGGAGTTC GAGTTGGATG TGCAAGGACT GCGCCAGGGT 
CTAAATGAGG CCTACGCCAT AGTGGCCGAG TGCCGTGGCA AGCTGGAGGT CTACGAGAAG 
CCCAACATCA CGCGGCTGAT GAACTCGAGT TCCTATGATC CTGCTGGACG GCGCATGCTG 
GCTCGTCTCC GGAGCTACGT GGCGGCCAAC GAGGCGCAAC TTCGTCTGGC CGAGCAGCAA 
TTGGATCTGC AGTGGGACCA GCTTCAAGAT GTGTTGCGGC GCAAGACCAA GTCGAGCATG 
CATATGCCGT GCCTGGAGGG GATCTACCAG CAACTGACGC GGCTCCAGAA CCTTGCCTCT 
GACCAGAGGA TCATGCAGAA CAGCATCAAG GCCAAGCTCA AGGAGCGTGG CCTGCTCCAG 
GCCGCACTTC TCGACCAGGA CAAGAGTCGC ACTCGCACCA ATGAAACTGT GGAAACCTTG 
GCTGACTCGA TTCTCTCGAT GAGTCTTAGT CAGACTGTCG ATACGAATGC CGCCAAGCTC 
ACACAGCACA AGCTCCGCCA GATCCGAAAC GTGGTGCAGC TGCAAAAGGT CAGCATCATT 
AGTCCCCAGC GTCCAGATCG TGTGGGGCTC AAGTCCGAAG TTATACTCGA GACCAAGCGC 
AAGGCAGAGA TATTGAAAAA GGCCGCCGCA AAGGCGACTA CTGCCAACAA GGCCACCCAG 
GTGGCGGCAC CGGCACCGGC ACCTGCACCT GCACCAACTC CAGCTCCAGC CGCTGCTCCT 
GTTGCGCCCC AACTACCAAA ACCAACAGCA TCCAAGCCCG CGGCCAGCAT CCCGGCCCTC 
AGTGCTGCAC CCATCGCAAC GCCATTCGCC TTCAGCCAGA GCAGTCCGTT TGTGAAGACT 
TCGAGTGTGA CCACGACAAC CACCACGGTA AGCTCTGGAG AGGCTCCAAA GCCGGCTGTC 
TCTTTGTTCG GAGGGCCAAG TAGGTCCGGC TTGAGCTTTG GACCGGTAGG AGGTGGTCTT 
TCGTTTGGGG GACTTTCTGC AGCTGATAAT TCAACTGGGG CCAAGCCGTC CCTGTTTGGA 
GGATTTGGGA TTAGTAAGGA GGCCACAGCA AAGCCGGCTC CTGAGCCAGC AAAGGCACAG 
GATCCCTTGG CGAAGGATAC TACACCTTTT ACTGCAGAAG TGGAGCCAGC GAAGGCACCG 
GAAACCCCAA AAGCAGAGAC TGCTGGCAAA TCCTTTTCCT TCACAGGATT CGGAGGAACT 
GGGGCTCCCA GTTCCACGTT TGGCTTCGGA GGACTGGGCT CTTCCCTTGG TTTTGGCGGC 
GTAGCAAAGA CCGATTCCAA CGCCGCAGTG ACGTCATCAG CTTCTGGCGT TCCCCGTGGC 
TTGTTCGCCT CAGCTGCAGC TGCACCGAAG CCTAACGAAG GTGATACAGC GGTGACCAGC 
ATCAGCACCA CCACCAAGCC AACGGTCGCA CCTGCGTCCA CCTTGTCTGC CGCTGCCGCT 
ACACCGTTGC CAACAAATAA CAGCGGAGTG ACCAGTACCG CCCCCAGCAG CTCTGATTCC 
CTTGGAGGGC TTTTCGGATC TGTCAATATA TGCAAGCCAC AAATCAAGGA ATCCTCCGGC 
GCTTCTGGAC AGCCTGCAAA TATCTTTGGC AGTGGATTCT CATTTGGCGG ACGAGGTGGA 
GATGAACCGA AGGGATTATT TGCGACCATC ACTCCAGCCA CAACAGCAAC AGCAACGGCA 
ACAGAAACAC CTGCAACCGC AGCACCAACT ACGATAAAAA TGGAGCCAGT GAGCACCACA 
GCCGCAACAA CTGCAGCACC TGCACCAGCT GCATCGGCTG CTCCGGCAAC AACCACTTCA 
ACTGAAACTA AGGGGGCTGC AACTCCACCC ACATCCACAC CGGCCGCTGC TCCGCCAGTA 
AAGGTTGCGG TTCCTCCGAA CACGACTTCA GCTGCAGCGC CAGCGGTCAC GCCTGCGACA 
GCAGTCTCTT CGACTGTGAC CTCGAGCACA GCGGTGCCAG GCAGTACATT CTCCTTCTCC 
AATGCATTCA GCAGCCTCAG CACCGCTCCG GCCACCCCCG TCACAGCCAC CAGCTCCAGT 
GCCTCTACAG TGAGCAATTC ATCGTCTTCG GTGTTTGGAG GATCCTTTAC AGCAGCACCA 
GCAACAACAG CGGCACCTGT AGCCAGCAAT CCATTCCAGG CATCGACAAC GGCTTCTGCA 
GCAACCTCCA CGAACCTTTT TGGAAATGTG GCCAAGTCCG AGACTAGCGT ATTTGGAGGT 
TCACCTGGAA CCAATCTCCC TCCTGCTGCA GCTGCTGCCC CACCCAGTGG TCTCTTTGCT 
GCAGCAGCTA TCAGTAATCC GGCGCCTTTT GGCAGCCCTC CACCGGGAAC GGCCGCCTCC 
GGGGGCAACA TCTTTAGCCA GGCCAACAAA CCAAGTGTTT TCGGTCAGCC GTCTGCAGGA 
GCGAGCGGCG GTACGTCTAT TTTTGGAGGG TCATCCGGAT CTCCATTTGG ATCGACCTCC 
ATTTTTGGCG GAAGCAATGC GCAAAGCCCT GCCAATGCAC CCACTGCCGG AAGTACATCC 
ATATTCGGTC AGAACGTCTT TGGGCAACCG GCGGCACAAA CAGCGACCCC AGTGGCAGGC 
GGTAATATAT TTGCAAGTCC TGTGAGCGCC AGCCAACAAC CGCAACCCTC TGCTTTTGGT 
GGAGGCGGAT CTATTTTTGG CGGACCGGCG ACGACTAGTT CTTCGCCTGC TTCTGGTGGA 
TCAATCTTTG GCGGTGGCAG CAGCTCTGGA TTTGGTTCAT TCTCCCAGAC TGCCCCTTCT 
CCTGGAGGCT TTGGCAGTGG CTTTGCTCAG GGCGCCGGAT CGTCAGTGGC CCAATCCGGC 
TTTGGATCAC CCCAAACGCC ACAGCAGGCT GCTCCGGGTG GATTTGGAGC CAAGCCCGTA 
TTCGGTGGTT CTCCGGCCTT CGGGGCCAGT CCCACCTTTG GGGGAGGCGC AACGTTTGGC 
AGTCCCAAGG GATTTGGCGG CTTTGGAGCC AGCCCTAACG TGGCCGCTCC ACCAGCCTTC 
GGAGCCCCTG CGAAACCGGC CGGCGGCAAC ATCTTCGACA CCTTGGGTGG TCAGGACTCC 
GGCTTGTCCT TTGGCAATCT GGCCCAGACC GGCAACCCCA ATGCCCAGAA GCCAGCTTTT 
GGCGGATCGA GTTTTATGAA TTATCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA17709 ( XM_015185236.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015040722.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA17709), mRNA.

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CloneID	ODe23557
Clone ID Related Accession (Same CDS sequence)	XM_015185236.1
Accession Version	XM_015185236.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCTAA GTGCTCCTGC CTGTAAGGAT ACACAGGACT TTCAATTTAA AGTGCATAAC 
AAGTATGCGG CGTTCAAAAA ATGCGGCGAG CCCCGCAGCA ATGTAAATCT TCTGGCGATT 
TCCTGCAGCC GCGGCTTGCT CTTTGCCGGC AACCCGACGC AGCCGGAACT TAAGGTGGTC 
ATCCTCAAGG ATTTGGCCAA TTCCAACAAG TCGCTCGGCG AGCAGCCGCA GGCGAGGAGT 
GTACCCCTGC CCAGCGTGCC CAACTATATC TCCTGCAGTG CCGATGGCAC TCTTCTGGCG 
GTTAACCTCA CACAGAATGG CACTGGCCTG CTCAACATTT ACTCGGTGCC GACATTCTTG 
GCCGCAGATG TGAAGCTCGT CTACAGCATT CGCCTGGCCA GCGAGGACTA TATCCATGCC 
GTGCAGCTGC TGTGGAATCC GGTCTTGCCC GGAAGTCTGG CCGTAGTGCT GAGCAACGGT 
GCTCTCGGCA TGTACACCCT GAAGGAGGGG GACACATTCG AGATGCATTC GCTGGACAAG 
AGTCAGCAAG TGAAGTGTGG CTGTTGGTCA CCCAAGGGAA AGCAAATTGT CCTAGGTTTT 
CCCGGTGGCA AGATACAGCA GTTCAAGCCG GATCTTACGC CAGCAAAGAC ACTGGTGTGT 
CCACCCAACA TCCACGATGC TCCGTTCGAC ACCATCGCCA TTCAGTGGCT CTCGACGTAC 
CAGTTCGCAG TGGTCTTCCT GCAGCATGGC GAGGACTGCA GTCCCGCCCT GTACATATTG 
AATGCCCCCA AGGGGGGATC GCCCAGCTAT ACCAACTACT ACGACATCTG CTACAGTCTC 
AACGGGCCAC GGAATCATCA GTTTTTCTTC AACCATGTGG CCCAGTGGAA TTTGCTTCTG 
GTGACATCGG CCAATGGCGT GGAAGTGGGT ATAATGGGTA CCTTCGAGTC TGGAGACACC 
CCCGTCTGGC AGCAGTTTAC CCTTCTGGAT GAGGCGAGGA TCGAGCTGCC TCTGAGCGAG 
GGCACCCAAG AGGAAACCTT CCCCCTAGGG TCTGTCTACG ACACATCCTC CACGCACCAA 
CTGACAATCA ACGAGCAGAA GCTCCCCACT ATGCCCATGG TGCATGTCTT AGGCACCGAC 
GGCACCCTTC TATCCTTCAA CTTCCTGAAT CTACTCCAAG GTGCCCCCTC TGTGTGCTCT 
CCGCCTCCGC CGGTGTTTGA CAGTTCGGGA CAGTTCCGCC CCCTGGACAC GTTACTGCCA 
GCGGAGGAAA AGGAGGCGGC CACAACACCG CCAGAAAAGG AGTCGACTCA GTCCGTTCCC 
ACGGACATGT CCTTTGCCTT CTCCCCGAAC ACGGTGACAT CGACTCCAGC TCCTGCCAAG 
GACAAGCCTT CCTCGCTGTT CTCCGGCTTC GCTGCACCCG CAGCAAGTAC TCCATTTGGA 
GTTGCTCCAA CTGCTGCTCC CACCGCGACA GTAGCGAGTA CCGCCTCGGC ATTCGGTGGG 
TTTGGTGCGG CCACGACGAC TCCAGCCTTA GGGTCCATAT TTTCCGCGCC AGGAGCGACC 
GCTTTTGGTG GTCTGGCTTT GGGTAAATCC AACGCAATGC CAGTGAGTGT GGCCAGTGCT 
CCTCTCGCCG TGCCACCGTC TGCAGCTGCT GCGCCCTTAA ACAAGCCCCT CTACACAGTT 
CCGCACACAT TTACGCCAGT GGCAACAAAG CAGCAACCGG CAGCGGCTCC CGTGTCCGTT 
CCATCAAGAC AGTCGCTGGA TAACACGGAG TCCATCGTTA GAGACATGAT AGCGCTACAG 
ATCGAAGCCT TCAACCAGGA CATACAGCAG CAGAAGGGAC TCACCACGGA GCTGCTCAGC 
ACCATTGCAG CTCCCTCGGC TCTCAAAAAG TATGCCAAGC GCTTGGACGA CCTCGAGGAG 
CTGAACGAGC AGGCCAAGGA CGTGGAGTTC GAGTTGGATG TGCAAGGACT GCGCCAGGGT 
CTAAATGAGG CCTACGCCAT AGTGGCCGAG TGCCGTGGCA AGCTGGAGGT CTACGAGAAG 
CCCAACATCA CGCGGCTGAT GAACTCGAGT TCCTATGATC CTGCTGGACG GCGCATGCTG 
GCTCGTCTCC GGAGCTACGT GGCGGCCAAC GAGGCGCAAC TTCGTCTGGC CGAGCAGCAA 
TTGGATCTGC AGTGGGACCA GCTTCAAGAT GTGTTGCGGC GCAAGACCAA GTCGAGCATG 
CATATGCCGT GCCTGGAGGG GATCTACCAG CAACTGACGC GGCTCCAGAA CCTTGCCTCT 
GACCAGAGGA TCATGCAGAA CAGCATCAAG GCCAAGCTCA AGGAGCGTGG CCTGCTCCAG 
GCCGCACTTC TCGACCAGGA CAAGAGTCGC ACTCGCACCA ATGAAACTGT GGAAACCTTG 
GCTGACTCGA TTCTCTCGAT GAGTCTTAGT CAGACTGTCG ATACGAATGC CGCCAAGCTC 
ACACAGCACA AGCTCCGCCA GATCCGAAAC GTGGTGCAGC TGCAAAAGGT CAGCATCATT 
AGTCCCCAGC GTCCAGATCG TGTGGGGCTC AAGTCCGAAG TTATACTCGA GACCAAGCGC 
AAGGCAGAGA TATTGAAAAA GGCCGCCGCA AAGGCGACTA CTGCCAACAA GGCCACCCAG 
GTGGCGGCAC CGGCACCGGC ACCTGCACCT GCACCAACTC CAGCTCCAGC CGCTGCTCCT 
GTTGCGCCCC AACTACCAAA ACCAACAGCA TCCAAGCCCG CGGCCAGCAT CCCGGCCCTC 
AGTGCTGCAC CCATCGCAAC GCCATTCGCC TTCAGCCAGA GCAGTCCGTT TGTGAAGACT 
TCGAGTGTGA CCACGACAAC CACCACGGTA AGCTCTGGAG AGGCTCCAAA GCCGGCTGTC 
TCTTTGTTCG GAGGGCCAAG TAGGTCCGGC TTGAGCTTTG GACCGGTAGG AGGTGGTCTT 
TCGTTTGGGG GACTTTCTGC AGCTGATAAT TCAACTGGGG CCAAGCCGTC CCTGTTTGGA 
GGATTTGGGA TTAGTAAGGA GGCCACAGGA TTCGGAGGAA CTGGGGCTCC CAGTTCCACG 
TTTGGCTTCG GAGGACTGGG CTCTTCCCTT GGTTTTGGCG GCGTAGCAAA GACCGATTCC 
AACGCCGCAG TGACGTCATC AGCTTCTGGC GTTCCCCGTG GCTTGTTCGC CTCAGCTGCA 
GCTGCACCGA AGCCTAACGA AGGTGATACA GCGGTGACCA GCATCAGCAC CACCACCAAG 
CCAACGGTCG CACCTGCGTC CACCTTGTCT GCCGCTGCCG CTACACCGTT GCCAACAAAT 
AACAGCGGAG TGACCAGTAC CGCCCCCAGC AGCTCTGATT CCCTTGGAGG GCTTTTCGGA 
TCTGTCAATA TATGCAAGCC ACAAATCAAG GAATCCTCCG GCGCTTCTGG ACAGCCTGCA 
AATATCTTTG GCAGTGGATT CTCATTTGGC GGACGAGGTG GAGATGAACC GAAGGGATTA 
TTTGCGACCA TCACTCCAGC CACAACAGCA ACAGCAACGG CAACAGAAAC ACCTGCAACC 
GCAGCACCAA CTACGATAAA AATGGAGCCA GTGAGCACCA CAGCCGCAAC AACTGCAGCA 
CCTGCACCAG CTGCATCGGC TGCTCCGGCA ACAACCACTT CAACTGAAAC TAAGGGGGCT 
GCAACTCCAC CCACATCCAC ACCGGCCGCT GCTCCGCCAG TAAAGGTTGC GGTTCCTCCG 
AACACGACTT CAGCTGCAGC GCCAGCGGTC ACGCCTGCGA CAGCAGTCTC TTCGACTGTG 
ACCTCGAGCA CAGCGGTGCC AGGCAGTACA TTCTCCTTCT CCAATGCATT CAGCAGCCTC 
AGCACCGCTC CGGCCACCCC CGTCACAGCC ACCAGCTCCA GTGCCTCTAC AGTGAGCAAT 
TCATCGTCTT CGGTGTTTGG AGGATCCTTT ACAGCAGCAC CAGCAACAAC AGCGGCACCT 
GTAGCCAGCA ATCCATTCCA GGCATCGACA ACGGCTTCTG CAGCAACCTC CACGAACCTT 
TTTGGAAATG TGGCCAAGTC CGAGACTAGC GTATTTGGAG GTTCACCTGG AACCAATCTC 
CCTCCTGCTG CAGCTGCTGC CCCACCCAGT GGTCTCTTTG CTGCAGCAGC TATCAGTAAT 
CCGGCGCCTT TTGGCAGCCC TCCACCGGGA ACGGCCGCCT CCGGGGGCAA CATCTTTAGC 
CAGGCCAACA AACCAAGTGT TTTCGGTCAG CCGTCTGCAG GAGCGAGCGG CGGTACGTCT 
ATTTTTGGAG GGTCATCCGG ATCTCCATTT GGATCGACCT CCATTTTTGG CGGAAGCAAT 
GCGCAAAGCC CTGCCAATGC ACCCACTGCC GGAAGTACAT CCATATTCGG TCAGAACGTC 
TTTGGGCAAC CGGCGGCACA AACAGCGACC CCAGTGGCAG GCGGTAATAT ATTTGCAAGT 
CCTGTGAGCG CCAGCCAACA ACCGCAACCC TCTGCTTTTG GTGGAGGCGG ATCTATTTTT 
GGCGGACCGG CGACGACTAG TTCTTCGCCT GCTTCTGGTG GATCAATCTT TGGCGGTGGC 
AGCAGCTCTG GATTTGGTTC ATTCTCCCAG ACTGCCCCTT CTCCTGGAGG CTTTGGCAGT 
GGCTTTGCTC AGGGCGCCGG ATCGTCAGTG GCCCAATCCG GCTTTGGATC ACCCCAAACG 
CCACAGCAGG CTGCTCCGGG TGGATTTGGA GCCAAGCCCG TATTCGGTGG TTCTCCGGCC 
TTCGGGGCCA GTCCCACCTT TGGGGGAGGC GCAACGTTTG GCAGTCCCAA GGGATTTGGC 
GGCTTTGGAG CCAGCCCTAA CGTGGCCGCT CCACCAGCCT TCGGAGCCCC TGCGAAACCG 
GCCGGCGGCA ACATCTTCGA CACCTTGGGT GGTCAGGACT CCGGCTTGTC CTTTGGCAAT 
CTGGCCCAGA CCGGCAACCC CAATGCCCAG AAGCCAGCTT TTGGCGGATC GAGTTTTATG 
AATTATCGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015040722.1
CDS	425..5416
Translation	MALSAPACKDTQDFQFKVHNKYAAFKKCGEPRSNVNLLAISCSRGLLFAGNPTQPELKVVILKDLANSNKSLGEQPQARSVPLPSVPNYISCSADGTLLAVNLTQNGTGLLNIYSVPTFLAADVKLVYSIRLASEDYIHAVQLLWNPVLPGSLAVVLSNGALGMYTLKEGDTFEMHSLDKSQQVKCGCWSPKGKQIVLGFPGGKIQQFKPDLTPAKTLVCPPNIHDAPFDTIAIQWLSTYQFAVVFLQHGEDCSPALYILNAPKGGSPSYTNYYDICYSLNGPRNHQFFFNHVAQWNLLLVTSANGVEVGIMGTFESGDTPVWQQFTLLDEARIELPLSEGTQEETFPLGSVYDTSSTHQLTINEQKLPTMPMVHVLGTDGTLLSFNFLNLLQGAPSVCSPPPPVFDSSGQFRPLDTLLPAEEKEAATTPPEKESTQSVPTDMSFAFSPNTVTSTPAPAKDKPSSLFSGFAAPAASTPFGVAPTAAPTATVASTASAFGGFGAATTTPALGSIFSAPGATAFGGLALGKSNAMPVSVASAPLAVPPSAAAAPLNKPLYTVPHTFTPVATKQQPAAAPVSVPSRQSLDNTESIVRDMIALQIEAFNQDIQQQKGLTTELLSTIAAPSALKKYAKRLDDLEELNEQAKDVEFELDVQGLRQGLNEAYAIVAECRGKLEVYEKPNITRLMNSSSYDPAGRRMLARLRSYVAANEAQLRLAEQQLDLQWDQLQDVLRRKTKSSMHMPCLEGIYQQLTRLQNLASDQRIMQNSIKAKLKERGLLQAALLDQDKSRTRTNETVETLADSILSMSLSQTVDTNAAKLTQHKLRQIRNVVQLQKVSIISPQRPDRVGLKSEVILETKRKAEILKKAAAKATTANKATQVAAPAPAPAPAPTPAPAAAPVAPQLPKPTASKPAASIPALSAAPIATPFAFSQSSPFVKTSSVTTTTTTVSSGEAPKPAVSLFGGPSRSGLSFGPVGGGLSFGGLSAADNSTGAKPSLFGGFGISKEATGFGGTGAPSSTFGFGGLGSSLGFGGVAKTDSNAAVTSSASGVPRGLFASAAAAPKPNEGDTAVTSISTTTKPTVAPASTLSAAAATPLPTNNSGVTSTAPSSSDSLGGLFGSVNICKPQIKESSGASGQPANIFGSGFSFGGRGGDEPKGLFATITPATTATATATETPATAAPTTIKMEPVSTTAATTAAPAPAASAAPATTTSTETKGAATPPTSTPAAAPPVKVAVPPNTTSAAAPAVTPATAVSSTVTSSTAVPGSTFSFSNAFSSLSTAPATPVTATSSSASTVSNSSSSVFGGSFTAAPATTAAPVASNPFQASTTASAATSTNLFGNVAKSETSVFGGSPGTNLPPAAAAAPPSGLFAAAAISNPAPFGSPPPGTAASGGNIFSQANKPSVFGQPSAGASGGTSIFGGSSGSPFGSTSIFGGSNAQSPANAPTAGSTSIFGQNVFGQPAAQTATPVAGGNIFASPVSASQQPQPSAFGGGGSIFGGPATTSSSPASGGSIFGGGSSSGFGSFSQTAPSPGGFGSGFAQGAGSSVAQSGFGSPQTPQQAAPGGFGAKPVFGGSPAFGASPTFGGGATFGSPKGFGGFGASPNVAAPPAFGAPAKPAGGNIFDTLGGQDSGLSFGNLAQTGNPNAQKPAFGGSSFMNYR

Target ORF information:

RefSeq Version	XM_015185236.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA17709), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015185236.1

ORF Insert Sequence:

ATGGCGCTAA GTGCTCCTGC CTGTAAGGAT ACACAGGACT TTCAATTTAA AGTGCATAAC 
AAGTATGCGG CGTTCAAAAA ATGCGGCGAG CCCCGCAGCA ATGTAAATCT TCTGGCGATT 
TCCTGCAGCC GCGGCTTGCT CTTTGCCGGC AACCCGACGC AGCCGGAACT TAAGGTGGTC 
ATCCTCAAGG ATTTGGCCAA TTCCAACAAG TCGCTCGGCG AGCAGCCGCA GGCGAGGAGT 
GTACCCCTGC CCAGCGTGCC CAACTATATC TCCTGCAGTG CCGATGGCAC TCTTCTGGCG 
GTTAACCTCA CACAGAATGG CACTGGCCTG CTCAACATTT ACTCGGTGCC GACATTCTTG 
GCCGCAGATG TGAAGCTCGT CTACAGCATT CGCCTGGCCA GCGAGGACTA TATCCATGCC 
GTGCAGCTGC TGTGGAATCC GGTCTTGCCC GGAAGTCTGG CCGTAGTGCT GAGCAACGGT 
GCTCTCGGCA TGTACACCCT GAAGGAGGGG GACACATTCG AGATGCATTC GCTGGACAAG 
AGTCAGCAAG TGAAGTGTGG CTGTTGGTCA CCCAAGGGAA AGCAAATTGT CCTAGGTTTT 
CCCGGTGGCA AGATACAGCA GTTCAAGCCG GATCTTACGC CAGCAAAGAC ACTGGTGTGT 
CCACCCAACA TCCACGATGC TCCGTTCGAC ACCATCGCCA TTCAGTGGCT CTCGACGTAC 
CAGTTCGCAG TGGTCTTCCT GCAGCATGGC GAGGACTGCA GTCCCGCCCT GTACATATTG 
AATGCCCCCA AGGGGGGATC GCCCAGCTAT ACCAACTACT ACGACATCTG CTACAGTCTC 
AACGGGCCAC GGAATCATCA GTTTTTCTTC AACCATGTGG CCCAGTGGAA TTTGCTTCTG 
GTGACATCGG CCAATGGCGT GGAAGTGGGT ATAATGGGTA CCTTCGAGTC TGGAGACACC 
CCCGTCTGGC AGCAGTTTAC CCTTCTGGAT GAGGCGAGGA TCGAGCTGCC TCTGAGCGAG 
GGCACCCAAG AGGAAACCTT CCCCCTAGGG TCTGTCTACG ACACATCCTC CACGCACCAA 
CTGACAATCA ACGAGCAGAA GCTCCCCACT ATGCCCATGG TGCATGTCTT AGGCACCGAC 
GGCACCCTTC TATCCTTCAA CTTCCTGAAT CTACTCCAAG GTGCCCCCTC TGTGTGCTCT 
CCGCCTCCGC CGGTGTTTGA CAGTTCGGGA CAGTTCCGCC CCCTGGACAC GTTACTGCCA 
GCGGAGGAAA AGGAGGCGGC CACAACACCG CCAGAAAAGG AGTCGACTCA GTCCGTTCCC 
ACGGACATGT CCTTTGCCTT CTCCCCGAAC ACGGTGACAT CGACTCCAGC TCCTGCCAAG 
GACAAGCCTT CCTCGCTGTT CTCCGGCTTC GCTGCACCCG CAGCAAGTAC TCCATTTGGA 
GTTGCTCCAA CTGCTGCTCC CACCGCGACA GTAGCGAGTA CCGCCTCGGC ATTCGGTGGG 
TTTGGTGCGG CCACGACGAC TCCAGCCTTA GGGTCCATAT TTTCCGCGCC AGGAGCGACC 
GCTTTTGGTG GTCTGGCTTT GGGTAAATCC AACGCAATGC CAGTGAGTGT GGCCAGTGCT 
CCTCTCGCCG TGCCACCGTC TGCAGCTGCT GCGCCCTTAA ACAAGCCCCT CTACACAGTT 
CCGCACACAT TTACGCCAGT GGCAACAAAG CAGCAACCGG CAGCGGCTCC CGTGTCCGTT 
CCATCAAGAC AGTCGCTGGA TAACACGGAG TCCATCGTTA GAGACATGAT AGCGCTACAG 
ATCGAAGCCT TCAACCAGGA CATACAGCAG CAGAAGGGAC TCACCACGGA GCTGCTCAGC 
ACCATTGCAG CTCCCTCGGC TCTCAAAAAG TATGCCAAGC GCTTGGACGA CCTCGAGGAG 
CTGAACGAGC AGGCCAAGGA CGTGGAGTTC GAGTTGGATG TGCAAGGACT GCGCCAGGGT 
CTAAATGAGG CCTACGCCAT AGTGGCCGAG TGCCGTGGCA AGCTGGAGGT CTACGAGAAG 
CCCAACATCA CGCGGCTGAT GAACTCGAGT TCCTATGATC CTGCTGGACG GCGCATGCTG 
GCTCGTCTCC GGAGCTACGT GGCGGCCAAC GAGGCGCAAC TTCGTCTGGC CGAGCAGCAA 
TTGGATCTGC AGTGGGACCA GCTTCAAGAT GTGTTGCGGC GCAAGACCAA GTCGAGCATG 
CATATGCCGT GCCTGGAGGG GATCTACCAG CAACTGACGC GGCTCCAGAA CCTTGCCTCT 
GACCAGAGGA TCATGCAGAA CAGCATCAAG GCCAAGCTCA AGGAGCGTGG CCTGCTCCAG 
GCCGCACTTC TCGACCAGGA CAAGAGTCGC ACTCGCACCA ATGAAACTGT GGAAACCTTG 
GCTGACTCGA TTCTCTCGAT GAGTCTTAGT CAGACTGTCG ATACGAATGC CGCCAAGCTC 
ACACAGCACA AGCTCCGCCA GATCCGAAAC GTGGTGCAGC TGCAAAAGGT CAGCATCATT 
AGTCCCCAGC GTCCAGATCG TGTGGGGCTC AAGTCCGAAG TTATACTCGA GACCAAGCGC 
AAGGCAGAGA TATTGAAAAA GGCCGCCGCA AAGGCGACTA CTGCCAACAA GGCCACCCAG 
GTGGCGGCAC CGGCACCGGC ACCTGCACCT GCACCAACTC CAGCTCCAGC CGCTGCTCCT 
GTTGCGCCCC AACTACCAAA ACCAACAGCA TCCAAGCCCG CGGCCAGCAT CCCGGCCCTC 
AGTGCTGCAC CCATCGCAAC GCCATTCGCC TTCAGCCAGA GCAGTCCGTT TGTGAAGACT 
TCGAGTGTGA CCACGACAAC CACCACGGTA AGCTCTGGAG AGGCTCCAAA GCCGGCTGTC 
TCTTTGTTCG GAGGGCCAAG TAGGTCCGGC TTGAGCTTTG GACCGGTAGG AGGTGGTCTT 
TCGTTTGGGG GACTTTCTGC AGCTGATAAT TCAACTGGGG CCAAGCCGTC CCTGTTTGGA 
GGATTTGGGA TTAGTAAGGA GGCCACAGGA TTCGGAGGAA CTGGGGCTCC CAGTTCCACG 
TTTGGCTTCG GAGGACTGGG CTCTTCCCTT GGTTTTGGCG GCGTAGCAAA GACCGATTCC 
AACGCCGCAG TGACGTCATC AGCTTCTGGC GTTCCCCGTG GCTTGTTCGC CTCAGCTGCA 
GCTGCACCGA AGCCTAACGA AGGTGATACA GCGGTGACCA GCATCAGCAC CACCACCAAG 
CCAACGGTCG CACCTGCGTC CACCTTGTCT GCCGCTGCCG CTACACCGTT GCCAACAAAT 
AACAGCGGAG TGACCAGTAC CGCCCCCAGC AGCTCTGATT CCCTTGGAGG GCTTTTCGGA 
TCTGTCAATA TATGCAAGCC ACAAATCAAG GAATCCTCCG GCGCTTCTGG ACAGCCTGCA 
AATATCTTTG GCAGTGGATT CTCATTTGGC GGACGAGGTG GAGATGAACC GAAGGGATTA 
TTTGCGACCA TCACTCCAGC CACAACAGCA ACAGCAACGG CAACAGAAAC ACCTGCAACC 
GCAGCACCAA CTACGATAAA AATGGAGCCA GTGAGCACCA CAGCCGCAAC AACTGCAGCA 
CCTGCACCAG CTGCATCGGC TGCTCCGGCA ACAACCACTT CAACTGAAAC TAAGGGGGCT 
GCAACTCCAC CCACATCCAC ACCGGCCGCT GCTCCGCCAG TAAAGGTTGC GGTTCCTCCG 
AACACGACTT CAGCTGCAGC GCCAGCGGTC ACGCCTGCGA CAGCAGTCTC TTCGACTGTG 
ACCTCGAGCA CAGCGGTGCC AGGCAGTACA TTCTCCTTCT CCAATGCATT CAGCAGCCTC 
AGCACCGCTC CGGCCACCCC CGTCACAGCC ACCAGCTCCA GTGCCTCTAC AGTGAGCAAT 
TCATCGTCTT CGGTGTTTGG AGGATCCTTT ACAGCAGCAC CAGCAACAAC AGCGGCACCT 
GTAGCCAGCA ATCCATTCCA GGCATCGACA ACGGCTTCTG CAGCAACCTC CACGAACCTT 
TTTGGAAATG TGGCCAAGTC CGAGACTAGC GTATTTGGAG GTTCACCTGG AACCAATCTC 
CCTCCTGCTG CAGCTGCTGC CCCACCCAGT GGTCTCTTTG CTGCAGCAGC TATCAGTAAT 
CCGGCGCCTT TTGGCAGCCC TCCACCGGGA ACGGCCGCCT CCGGGGGCAA CATCTTTAGC 
CAGGCCAACA AACCAAGTGT TTTCGGTCAG CCGTCTGCAG GAGCGAGCGG CGGTACGTCT 
ATTTTTGGAG GGTCATCCGG ATCTCCATTT GGATCGACCT CCATTTTTGG CGGAAGCAAT 
GCGCAAAGCC CTGCCAATGC ACCCACTGCC GGAAGTACAT CCATATTCGG TCAGAACGTC 
TTTGGGCAAC CGGCGGCACA AACAGCGACC CCAGTGGCAG GCGGTAATAT ATTTGCAAGT 
CCTGTGAGCG CCAGCCAACA ACCGCAACCC TCTGCTTTTG GTGGAGGCGG ATCTATTTTT 
GGCGGACCGG CGACGACTAG TTCTTCGCCT GCTTCTGGTG GATCAATCTT TGGCGGTGGC 
AGCAGCTCTG GATTTGGTTC ATTCTCCCAG ACTGCCCCTT CTCCTGGAGG CTTTGGCAGT 
GGCTTTGCTC AGGGCGCCGG ATCGTCAGTG GCCCAATCCG GCTTTGGATC ACCCCAAACG 
CCACAGCAGG CTGCTCCGGG TGGATTTGGA GCCAAGCCCG TATTCGGTGG TTCTCCGGCC 
TTCGGGGCCA GTCCCACCTT TGGGGGAGGC GCAACGTTTG GCAGTCCCAA GGGATTTGGC 
GGCTTTGGAG CCAGCCCTAA CGTGGCCGCT CCACCAGCCT TCGGAGCCCC TGCGAAACCG 
GCCGGCGGCA ACATCTTCGA CACCTTGGGT GGTCAGGACT CCGGCTTGTC CTTTGGCAAT 
CTGGCCCAGA CCGGCAACCC CAATGCCCAG AAGCCAGCTT TTGGCGGATC GAGTTTTATG 
AATTATCGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA17709 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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