Dpse\GA12265 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA12265
Entrez Gene ID	4801959
Full Name	GA12265 gene product from transcript GA12265-RB
Synonyms	GA12265,dpse_GLEANR_3700
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001358937.3	XP_001358974.1	uncharacterized protein, isoform A
XM_015182015.1	XP_015037501.1	uncharacterized protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA12265 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA12265 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe01527	XM_001358937.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA12265), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ODe20357	XM_015182015.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA12265), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA12265 ( XM_001358937.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001358974.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA12265), mRNA.

Price & Availability↑

CloneID	ODe01527
Clone ID Related Accession (Same CDS sequence)	XM_001358937.3
Accession Version	XM_001358937.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). On Dec 22, 2015 this sequence version replaced XM_001358937.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAAGC CACACTCGGA CGGAGATGCC TCTGGTCTGG GCTATGCCTA CCAGCTGCCG 
TCCGGAGGCC TCCTGCTGCG CCTCAAGTCC TCTCTGTCGC AGTTCAGCGA TCTGTTCAGC 
GACTTTAGCA AGTACATCTC GCCCGCCTAC CATCACGACC GTTGCGAGCG ATCCGTGCAT 
ATGCGTCTCT ACTCGATGCA CAAGCGGGAG TTCGTCATGG TATTTCTGGG CTTCTTCACC 
TGCTTCGGAC TGGGCATCAT TATTGGCCTC ACAGGGCCGC CCATCACCAG CACTTCGACG 
CTGACGGCCC AACAAGTGCT GGCCAACACA ACACTGGCCC ACAGTCCCAC GGTTCTGGCC 
ACAGGGCCCT TTGCCATGAA GTCCCCGCTG ACCACCACGT ACTCACAGCA GCTGTGGCTG 
ATAGCCAAAC TGGTGACGGA CAACAACGAT GATGAACGCT ATGACAAGAG TTTCCAGGTG 
AGCGTATCCA TCGATGGCAT CAATGAACAG CACAAGCCGC TCAGTGTTCT GGGCTCTGGA 
ATGGCCCACA ATCGGACGCG GCATTTGGTC TGTGCGCGTA ACGATTGCGA GGAGTTCACG 
GTGATGCACT TGGGCTTCCT CGATTATGCC CACTACATCA TCACGGTGCG GTTCTTTGGC 
CTAGAGTCCT TTCATCAAAA GTACAACATC CGCAGCATTA CCTTCTATTT CAAGACGTAC 
AGCCCTGAGT TTACACAGAT TGAGATCTGG TTTAGGTGCA TCTTTCTGTT GTTTACCTTC 
ATTGTTATAT GCTGGTATGC CCACACCCTG CGCAAATATC CCGTCTACGA TTGGTCCATA 
GAGCAGCGGT GGCTATCGGT TCTACTGCCC CTGCTCCTTC TCTACGATAA TCCCTTCTTT 
CCGCTGATCT TTCTAATGAA CTCTTGGCTG CCTGGAATGC TGGATGCCAT TCTACAGGCC 
ACTTTCCTGT GTGCCCTGCT CATGTTTTGG CTGTGCATCT ACCATGGCCT GCGCCAGAAC 
GAGCGCAGCT TTGTGCGTTT CTATTTGGTT AAGGTGATTG TCGTGCTGCC CATTTGGCTG 
TGCGCCATTG TGCTGGCCAC CTGGGAGAAA TGCAACGAGC TGAGGGACCC CACCTACAGT 
CACTTCGTGG ACACCAGGAA CTACAATGGC TTCAAGCTGT TCTTCTACAT TGCCTGCTGC 
ATGTATGTGC TGTACTTGGT CCTGCTGCTA CTCCGTGCCT ACACCGAGCT GCGCTCCATG 
CCCTACTTTG ACATGCGCCT CAAGTTCCTG ACGCTGCTGA TGCTCTTCGT GCTGGCCATT 
TCCATCACGG TGACCACCAG CCGTTTCGGT TTTGGGATAC TGGAGGACAA CTTTGTGGCC 
TCACTGAACA CCACCTACCG CAGCTCCGCC CAGTTCATGT GCTTCTACGG CCTCCTTAAC 
TTCTATCTGT ACACCATGGC GTATGTGTAT TCCCCCGATG GACGCTTCTC CCAGGCCGAG 
CTGGCCGTCA CCAAGGACAA TCCCGCCATT TCCATGATCG ATGACTCCGA CGAGGATGTG 
GTCTATGGCT CCGATGAGGA GTCACGACGC CCGCTCACCT CGGTGGGTGG AGGGGGTGGT 
AAAAACGAGT ACGACAGCGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001358974.1
CDS	137..1780
Translation	MAKPHSDGDASGLGYAYQLPSGGLLLRLKSSLSQFSDLFSDFSKYISPAYHHDRCERSVHMRLYSMHKREFVMVFLGFFTCFGLGIIIGLTGPPITSTSTLTAQQVLANTTLAHSPTVLATGPFAMKSPLTTTYSQQLWLIAKLVTDNNDDERYDKSFQVSVSIDGINEQHKPLSVLGSGMAHNRTRHLVCARNDCEEFTVMHLGFLDYAHYIITVRFFGLESFHQKYNIRSITFYFKTYSPEFTQIEIWFRCIFLLFTFIVICWYAHTLRKYPVYDWSIEQRWLSVLLPLLLLYDNPFFPLIFLMNSWLPGMLDAILQATFLCALLMFWLCIYHGLRQNERSFVRFYLVKVIVVLPIWLCAIVLATWEKCNELRDPTYSHFVDTRNYNGFKLFFYIACCMYVLYLVLLLLRAYTELRSMPYFDMRLKFLTLLMLFVLAISITVTTSRFGFGILEDNFVASLNTTYRSSAQFMCFYGLLNFYLYTMAYVYSPDGRFSQAELAVTKDNPAISMIDDSDEDVVYGSDEESRRPLTSVGGGGGKNEYDSD

Target ORF information:

RefSeq Version	XM_001358937.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA12265), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001358937.3

ORF Insert Sequence:

ATGGCCAAGC CACACTCGGA CGGAGATGCC TCTGGTCTGG GCTATGCCTA CCAGCTGCCG 
TCCGGAGGCC TCCTGCTGCG CCTCAAGTCC TCTCTGTCGC AGTTCAGCGA TCTGTTCAGC 
GACTTTAGCA AGTACATCTC GCCCGCCTAC CATCACGACC GTTGCGAGCG ATCCGTGCAT 
ATGCGTCTCT ACTCGATGCA CAAGCGGGAG TTCGTCATGG TATTTCTGGG CTTCTTCACC 
TGCTTCGGAC TGGGCATCAT TATTGGCCTC ACAGGGCCGC CCATCACCAG CACTTCGACG 
CTGACGGCCC AACAAGTGCT GGCCAACACA ACACTGGCCC ACAGTCCCAC GGTTCTGGCC 
ACAGGGCCCT TTGCCATGAA GTCCCCGCTG ACCACCACGT ACTCACAGCA GCTGTGGCTG 
ATAGCCAAAC TGGTGACGGA CAACAACGAT GATGAACGCT ATGACAAGAG TTTCCAGGTG 
AGCGTATCCA TCGATGGCAT CAATGAACAG CACAAGCCGC TCAGTGTTCT GGGCTCTGGA 
ATGGCCCACA ATCGGACGCG GCATTTGGTC TGTGCGCGTA ACGATTGCGA GGAGTTCACG 
GTGATGCACT TGGGCTTCCT CGATTATGCC CACTACATCA TCACGGTGCG GTTCTTTGGC 
CTAGAGTCCT TTCATCAAAA GTACAACATC CGCAGCATTA CCTTCTATTT CAAGACGTAC 
AGCCCTGAGT TTACACAGAT TGAGATCTGG TTTAGGTGCA TCTTTCTGTT GTTTACCTTC 
ATTGTTATAT GCTGGTATGC CCACACCCTG CGCAAATATC CCGTCTACGA TTGGTCCATA 
GAGCAGCGGT GGCTATCGGT TCTACTGCCC CTGCTCCTTC TCTACGATAA TCCCTTCTTT 
CCGCTGATCT TTCTAATGAA CTCTTGGCTG CCTGGAATGC TGGATGCCAT TCTACAGGCC 
ACTTTCCTGT GTGCCCTGCT CATGTTTTGG CTGTGCATCT ACCATGGCCT GCGCCAGAAC 
GAGCGCAGCT TTGTGCGTTT CTATTTGGTT AAGGTGATTG TCGTGCTGCC CATTTGGCTG 
TGCGCCATTG TGCTGGCCAC CTGGGAGAAA TGCAACGAGC TGAGGGACCC CACCTACAGT 
CACTTCGTGG ACACCAGGAA CTACAATGGC TTCAAGCTGT TCTTCTACAT TGCCTGCTGC 
ATGTATGTGC TGTACTTGGT CCTGCTGCTA CTCCGTGCCT ACACCGAGCT GCGCTCCATG 
CCCTACTTTG ACATGCGCCT CAAGTTCCTG ACGCTGCTGA TGCTCTTCGT GCTGGCCATT 
TCCATCACGG TGACCACCAG CCGTTTCGGT TTTGGGATAC TGGAGGACAA CTTTGTGGCC 
TCACTGAACA CCACCTACCG CAGCTCCGCC CAGTTCATGT GCTTCTACGG CCTCCTTAAC 
TTCTATCTGT ACACCATGGC GTATGTGTAT TCCCCCGATG GACGCTTCTC CCAGGCCGAG 
CTGGCCGTCA CCAAGGACAA TCCCGCCATT TCCATGATCG ATGACTCCGA CGAGGATGTG 
GTCTATGGCT CCGATGAGGA GTCACGACGC CCGCTCACCT CGGTGGGTGG AGGGGGTGGT 
AAAAACGAGT ACGACAGCGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA12265 ( XM_015182015.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015037501.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA12265), mRNA.

Price & Availability↑

CloneID	ODe20357
Clone ID Related Accession (Same CDS sequence)	XM_015182015.1
Accession Version	XM_015182015.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAAGC CACACTCGGA CGGAGATGCC TCTGGTCTGG GCTATGCCTA CCAGCTGCCG 
TCCGGAGGCC TCCTGCTGCG CCTCAAGTCC TCTCTGTCGC AGTTCAGCGA TCTGTTCAGC 
GACTTTAGCA AGTACATCTC GCCCGCCTAC CATCACGACC GTTGCGAGCG ATCCGTGCAT 
ATGCGTCTCT ACTCGATGCA CAAGCGGGAG TTCGTCATGG TATTTCTGGG CTTCTTCACC 
TGCTTCGGAC TGGGCATCAT TATTGGCCTC ACAGGGCCGC CCATCACCAG CACTTCGACG 
CTGACGGCCC AACAAGTGCT GGCCAACACA ACACTGGCCC ACAGTCCCAC GGTTCTGGCC 
ACAGGGCCCT TTGCCATGAA GTCCCCGCTG ACCACCACGT ACTCACAGCA GCTGTGGCTG 
ATAGCCAAAC TGGTGACGGA CAACAACGAT GATGAACGCT ATGACAAGAG TTTCCAGGTG 
AGCGTATCCA TCGATGGCAT CAATGAACAG CACAAGCCGC TCAGTGTTCT GGGCTCTGGA 
ATGGCCCACA ATCGGACGCG GCATTTGGTC TGTGCGCGTA ACGATTGCGA GGAGTTCACG 
GTGATGCACT TGGGCTTCCT CGATTATGCC CACTACATCA TCACGGTGCG GTTCTTTGGC 
CTAGAGTCCT TTCATCAAAA GTACAACATC CGCAGCATTA CCTTCTATTG CTGGTATGCC 
CACACCCTGC GCAAATATCC CGTCTACGAT TGGTCCATAG AGCAGCGGTG GCTATCGGTT 
CTACTGCCCC TGCTCCTTCT CTACGATAAT CCCTTCTTTC CGCTGATCTT TCTAATGAAC 
TCTTGGCTGC CTGGAATGCT GGATGCCATT CTACAGGCCA CTTTCCTGTG TGCCCTGCTC 
ATGTTTTGGC TGTGCATCTA CCATGGCCTG CGCCAGAACG AGCGCAGCTT TGTGCGTTTC 
TATTTGGTTA AGGTGATTGT CGTGCTGCCC ATTTGGCTGT GCGCCATTGT GCTGGCCACC 
TGGGAGAAAT GCAACGAGCT GAGGGACCCC ACCTACAGTC ACTTCGTGGA CACCAGGAAC 
TACAATGGCT TCAAGCTGTT CTTCTACATT GCCTGCTGCA TGTATGTGCT GTACTTGGTC 
CTGCTGCTAC TCCGTGCCTA CACCGAGCTG CGCTCCATGC CCTACTTTGA CATGCGCCTC 
AAGTTCCTGA CGCTGCTGAT GCTCTTCGTG CTGGCCATTT CCATCACGGT GACCACCAGC 
CGTTTCGGTT TTGGGATACT GGAGGACAAC TTTGTGGCCT CACTGAACAC CACCTACCGC 
AGCTCCGCCC AGTTCATGTG CTTCTACGGC CTCCTTAACT TCTATCTGTA CACCATGGCG 
TATGTGTATT CCCCCGATGG ACGCTTCTCC CAGGCCGAGC TGGCCGTCAC CAAGGACAAT 
CCCGCCATTT CCATGATCGA TGACTCCGAC GAGGATGTGG TCTATGGCTC CGATGAGGAG 
TCACGACGCC CGCTCACCTC GGTGGGTGGA GGGGGTGGTA AAAACGAGTA CGACAGCGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015037501.1
CDS	138..1700
Translation	MAKPHSDGDASGLGYAYQLPSGGLLLRLKSSLSQFSDLFSDFSKYISPAYHHDRCERSVHMRLYSMHKREFVMVFLGFFTCFGLGIIIGLTGPPITSTSTLTAQQVLANTTLAHSPTVLATGPFAMKSPLTTTYSQQLWLIAKLVTDNNDDERYDKSFQVSVSIDGINEQHKPLSVLGSGMAHNRTRHLVCARNDCEEFTVMHLGFLDYAHYIITVRFFGLESFHQKYNIRSITFYCWYAHTLRKYPVYDWSIEQRWLSVLLPLLLLYDNPFFPLIFLMNSWLPGMLDAILQATFLCALLMFWLCIYHGLRQNERSFVRFYLVKVIVVLPIWLCAIVLATWEKCNELRDPTYSHFVDTRNYNGFKLFFYIACCMYVLYLVLLLLRAYTELRSMPYFDMRLKFLTLLMLFVLAISITVTTSRFGFGILEDNFVASLNTTYRSSAQFMCFYGLLNFYLYTMAYVYSPDGRFSQAELAVTKDNPAISMIDDSDEDVVYGSDEESRRPLTSVGGGGGKNEYDSD

Target ORF information:

RefSeq Version	XM_015182015.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA12265), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015182015.1

ORF Insert Sequence:

ATGGCCAAGC CACACTCGGA CGGAGATGCC TCTGGTCTGG GCTATGCCTA CCAGCTGCCG 
TCCGGAGGCC TCCTGCTGCG CCTCAAGTCC TCTCTGTCGC AGTTCAGCGA TCTGTTCAGC 
GACTTTAGCA AGTACATCTC GCCCGCCTAC CATCACGACC GTTGCGAGCG ATCCGTGCAT 
ATGCGTCTCT ACTCGATGCA CAAGCGGGAG TTCGTCATGG TATTTCTGGG CTTCTTCACC 
TGCTTCGGAC TGGGCATCAT TATTGGCCTC ACAGGGCCGC CCATCACCAG CACTTCGACG 
CTGACGGCCC AACAAGTGCT GGCCAACACA ACACTGGCCC ACAGTCCCAC GGTTCTGGCC 
ACAGGGCCCT TTGCCATGAA GTCCCCGCTG ACCACCACGT ACTCACAGCA GCTGTGGCTG 
ATAGCCAAAC TGGTGACGGA CAACAACGAT GATGAACGCT ATGACAAGAG TTTCCAGGTG 
AGCGTATCCA TCGATGGCAT CAATGAACAG CACAAGCCGC TCAGTGTTCT GGGCTCTGGA 
ATGGCCCACA ATCGGACGCG GCATTTGGTC TGTGCGCGTA ACGATTGCGA GGAGTTCACG 
GTGATGCACT TGGGCTTCCT CGATTATGCC CACTACATCA TCACGGTGCG GTTCTTTGGC 
CTAGAGTCCT TTCATCAAAA GTACAACATC CGCAGCATTA CCTTCTATTG CTGGTATGCC 
CACACCCTGC GCAAATATCC CGTCTACGAT TGGTCCATAG AGCAGCGGTG GCTATCGGTT 
CTACTGCCCC TGCTCCTTCT CTACGATAAT CCCTTCTTTC CGCTGATCTT TCTAATGAAC 
TCTTGGCTGC CTGGAATGCT GGATGCCATT CTACAGGCCA CTTTCCTGTG TGCCCTGCTC 
ATGTTTTGGC TGTGCATCTA CCATGGCCTG CGCCAGAACG AGCGCAGCTT TGTGCGTTTC 
TATTTGGTTA AGGTGATTGT CGTGCTGCCC ATTTGGCTGT GCGCCATTGT GCTGGCCACC 
TGGGAGAAAT GCAACGAGCT GAGGGACCCC ACCTACAGTC ACTTCGTGGA CACCAGGAAC 
TACAATGGCT TCAAGCTGTT CTTCTACATT GCCTGCTGCA TGTATGTGCT GTACTTGGTC 
CTGCTGCTAC TCCGTGCCTA CACCGAGCTG CGCTCCATGC CCTACTTTGA CATGCGCCTC 
AAGTTCCTGA CGCTGCTGAT GCTCTTCGTG CTGGCCATTT CCATCACGGT GACCACCAGC 
CGTTTCGGTT TTGGGATACT GGAGGACAAC TTTGTGGCCT CACTGAACAC CACCTACCGC 
AGCTCCGCCC AGTTCATGTG CTTCTACGGC CTCCTTAACT TCTATCTGTA CACCATGGCG 
TATGTGTATT CCCCCGATGG ACGCTTCTCC CAGGCCGAGC TGGCCGTCAC CAAGGACAAT 
CCCGCCATTT CCATGATCGA TGACTCCGAC GAGGATGTGG TCTATGGCTC CGATGAGGAG 
TCACGACGCC CGCTCACCTC GGTGGGTGGA GGGGGTGGTA AAAACGAGTA CGACAGCGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA12265 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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