LOC100185798 cDNA ORF clone, Ciona intestinalis(vase tunicate)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100185798
Entrez Gene ID	100185798
Full Name	uncharacterized LOC100185798
Gene Type	protein-coding
Organism	Ciona intestinalis(vase tunicate)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018813181.1	XP_018668726.1	uncharacterized protein LOC100185798
XM_026835808.1	XP_026691609.1	coiled-coil domain-containing protein CG32809 isoform X3
XM_026835809.1	XP_026691610.1	coiled-coil domain-containing protein CG32809 isoform X4
XM_018813181.2	XP_018668726.1	uncharacterized protein LOC100185798 isoform X1
XM_026835807.1	XP_026691608.1	uncharacterized protein LOC100185798 isoform X2
XM_026835810.1	XP_026691611.1	uncharacterized protein LOC100185798 isoform X5

Pathways from BioSystems
Gene Ontology

The following LOC100185798 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100185798 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCi156734	XM_026835807.1 Latest version!	Ciona intestinalis coiled-coil domain-containing protein CG32809 (LOC100185798), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCi156735	XM_026835808.1 Latest version!	Ciona intestinalis coiled-coil domain-containing protein CG32809 (LOC100185798), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCi156736	XM_026835809.1 Latest version!	Ciona intestinalis coiled-coil domain-containing protein CG32809 (LOC100185798), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCi156737	XM_026835810.1 Latest version!	Ciona intestinalis coiled-coil domain-containing protein CG32809 (LOC100185798), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCi74452	XM_018813181.2 Latest version!	Ciona intestinalis coiled-coil domain-containing protein CG32809 (LOC100185798), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCi74452	XM_018813181.1	Ciona intestinalis uncharacterized LOC100185798 (LOC100185798), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100185798 ( XM_026835807.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_026691608.1 Ciona intestinalis coiled-coil domain-containing protein CG32809 (LOC100185798), transcript variant X2, mRNA.

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CloneID	OCi156734
Clone ID Related Accession (Same CDS sequence)	XM_026835807.1
Accession Version	XM_026835807.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1540051200000
Organism	Ciona intestinalis(vase tunicate)
Product	uncharacterized protein LOC100185798 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020174.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ciona intestinalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAA ATACACAAGA AGCTGGCGAC TCAGAAGTCG AAAATGACAA GGCAAGTTAC 
GCGATGTTGA GACCCTTGCA GAAAGTGATC GCTCACTTTG AGATGAAACT TGCCGCCAAC 
AAAGGGAACA TTCCAACACC CAGACGAGAA ATACCAAACC TGAAACCGCA AGCTGAGCGT 
CTTCGTCAGA GCAGGAAAAC GTCCAAACAA CGAAGTTTTA TTAAAAGCAG TGGGAAGCTA 
TTGTCAAGCG TGCAAGAGAA GGTTACTGTT GGATCTAAAT CGTCAACACC TTGTCATAGC 
AGATCAAGTT CGGTGTGTTC AAGTAATGTG GAAAGTCATG TACCTACGTC AGAAGGCCCT 
CCCATGACAT CATCGCAACA AGATTTTGAC ACCACTTCTG TTTCGTCTAC GTCCAGTATG 
ACGTCAGAAA CTGTAGATTC TCAAGTTAAA TATTCCTCGT TACAAAGAAA CTACCAACAT 
GGGCACAAGA GTTTTCAGTT CAACACTCTG GATGGAAGGA GAACTCGATC TAAGAGTGGA 
GGCCACCATA ATCGAAGTTT CTATCCGCAA GATGGCAGTA TATCCCCAGC CGAAGCAGAT 
CGAAAGCGCT CAGCCTTTTT TCAATATTTA GTCCAAAAAT ACCCGCAACA CTCCGATGTT 
ATTGTTGGGA AATCATTTCA GCAAACCCAG ACTCCAGCAC CTTCCTTCGG TGGGATTCGA 
ACCCGTGCAA GCTACGCTGC CGATTGCTCT TATAACTCTT CATCTCCTAA CGACAGCGAG 
GTCACGTGGC ATAACAGTTT GTCACGTGGT AGTAGACTCA GGTCCAGCCT CCCAATCGTA 
AGAAGTTTAA GTCGAAGCCG AGAACGACCA TTAGGCACCA TTTACTTGGA GTATAGCGGA 
GAGTGTAGAA CTGCCAGACT GCCGAACGAA GTGACAAGCC TTGATACAAT ACGAGCTCTC 
TTCGTACAAG CATACCCAGG ATTGCTAACG ATGCCAATGC TTGACCCACA ACGTCACTCC 
ATTTTATTAA AAAACAGGAA GACGGGAAAC TACGATGAGA TCGATAACGT GAGTCGCATC 
AAGGATCGTT CATTCATTCG AACTCGTCCG ATCGGGGGTT CAGGTATTCC ATTGCCTGGG 
ACCAAGAAGC CTACAATCAA CCACCCGAAG CCTGCTGCTA AACCAGTTAA GCCAATACCG 
GGCAACAACC GATCAAAACC ACGGGTTACT GATGATGTAA AAATTGCAGA ACGCAACCAA 
AAGAACTTGA ACAAAAGTGG GATAAAAGCA CCGGTTAAAT CCCTGATCCG TCAATACCAG 
TCTGCACCTA GTTTAATAAA GCAAGCAGTG ACCGGTTCAA ACGAGATAAA ACCTGTTCAA 
AGTGGCGAGG AGACTCAGGG TAAAGTGAAA CCGCCTCCCC CACCGCGGTC CGCAAAGCCA 
ACAGTCGTAG CACAAACAGA AGCAGCCCAA GATCAAGGCA ATTATTCGCC TAACGGGACT 
CTATTATCAG GCAATGGGAT TGGGGGTGGT AGTATACCAC AGAAAACTTT CTCGTACGTT 
AAAAGCGGCA ATGAGTCTCC AGTAAACCAC CAACAACAGA CAATCACATC CCCACCGCAG 
CATAGGGGCC TGAGGCAACC TACAACTTCC AGGCTTTCCC CTCCATCTGT TGAGAAGAAG 
TTTTCCTCCA TACCAACGCC AGGGTCAAAG CCGAAGATCC AACTCCCTCC GAATCCAACA 
AAACCAACCG ATGACGAACA CCACACTAAG GAGAACGTGC CACGCCCAAA GACTCCCAAC 
ATACTCCACC AATCACCGCC CACCGTCACC AAACTTCCGC CTACGTCATT CGCAATGACG 
TCACCGAAAA ATGGCGCTCA ACGCCTTCCC CAACCAAAAC CTTTCGGCAT GAAACAACAT 
ACAACGCCAG CGAACCGGTT CGGTGCAAAA CGAACGTCCC CTCCAAACGT CCCAAACAAT 
AATCAATTGG TGCCGGAAAC TGCGCCGGTC TATTCAAATA TAAAGCGGTC AGCCTCACCG 
GAAAGAAGAG CTACAATAAA CGGTACCGGT GGATCAGCAG GTTACTGCAG GGGTGGTAGG 
TCCTTTAAAG GCCACCAGCT GAAACCTGTA AATATTCCAT CCGTTCCCGA CACTGAAATA 
GCTGCAGCTT CTAACCTGGA AGCCAAACCT GATCTCAACG TGCCGGAGGT AGCTCAACCT 
GTAGAACCGC GGCAACCCAT TTCATTACCC AGTGATACCA AATACTCTGC CATGCTACCG 
AACCCTTGTA CCACAGATCA AGTGACGTCA TGCAGCTCCT CTACGTCATC CGATGACGTC 
ACCGACCCTG GAAAATCAAA GATGTCAACG AAGAAGGGAT TGTTCGGGCT TCGGAGATTG 
GTTTCGAACC GAAACAAGAA CCCACAAAAT ATTCCGAAAC GAAGTCAAAG CGAGGAGCGA 
CCGAAGACAT CGGATCCTTC GGTAAATAAA TCCGACCTTC ATTCTCCATC TTTCAACGAG 
TCCTTGGCTT CGTTTAGGTC ATCGTCCCCG GCTCTTCAAG TCAGCACGCC AGTGGTTCGA 
TCTTCACCCA TTATGAGCAA GTCTCCTGCC ATACCGAAAA GTGATCTTCT AAATAGTTTG 
CTTACAACCA ACAAGGAGAC ACCTGAGCCG AACACAGATG TTATGTCCAC TTCCAGCTCG 
GATGACGACA AGGACAAACC TGCTCCTCAC CCCATACTGG CTGGAGTGCT TAAAAACAAC 
AACTTGGTCA AAAGCACTTC TTCCAGTTCG ATTTTGTCCG ACTCCGGCGC AAGTAACGTG 
TCTATGACGT CATCCATACG TACAAAGAAG GTTTCATTTC AAGACGACGT AACAGTTTGT 
GATGGTAATG GGATCAGTAC GTTTAGTAAC GGATTGCGAG AACCTGACGC TTCTGTAGCT 
GATGTATCGT TCGATAGGTC GATGGAACAA TCGTTATCTG TCGACACGCA TGAGGGTGCG 
GAGAATGAGC CAAAGCAGTG GCGACCGCCC TCTAACGGTC CGCCTGCTCG AAATGATTTC 
CAACAGGTGG GCTACAGAGC AAGACCCGCA ACACCACACA GGTATGAACC ACCACCATAC 
CAACCCTCCC CTCTTGCATA CCAAACCAAC TCTCCTCGAT CGCACCAATC AGCTGTGGCC 
GCTGCTATAC AAAGCATAAG TGGGAATGCG GAAACAACAA GCAGTGCTGC AAGGTACATT 
CCAAGCCAAC CTGAACATTT CTCGGTATCT TGCGCATCAC CTGTGCCAGT AGTAACCAGG 
CAGGGGCACG AGGCTCCACA CAAGGAGGAA TGGAATCCTC TCCGACGACA ATCCGCAGAC 
GCAAGCTCTA ACTCACCTTC AATTACACAG CGGACATGTC CTACCCCTGT GCCAACCGCC 
CCGCATAAGT TAGCTGAGGT ACAAGTAGAA AACGAGCCTT CATATTTCCA AACAGAGCGA 
GCGCCATCTC GTTCTGAAAC ATTTAACTCC GACACACCCC CCTACTACGA ATGCTGCGAA 
ACTACGACGC CCGCCTCTGG CTCAATTACA CCAAGTCAAT TAAGAGATGC GTTAAAGACA 
CAGGACGAAC AGTATCAACA TCCAGTCGTT GAATCTGCGG GCCCTGAGAA TCAAAGTTCT 
GTCACATTTT CCTCTCTCCC TTTATCTGTC CCACCATCAG GGAACGTTTT GCCACAACCC 
GAAACCGATG TTACGAACAA GAAGGTCATG GCTATGGAGC GCCAACTAGC GAGTTTAACC 
GGCATGGTTG AATCCATTAT AACCAGTACC GACGGTGTTC AAACCAACAA GCCTGAACCC 
CCATACCAGA ATCAACCAGT CGGTGGGAAT CTCCCTAAAA CGTCACACAC CGTCCAACAA 
GTGATGACGT CATCAGAGAT GGGACAACAA CTCTTGAAGT TGAGGGAAAC AGCTCATAAT 
CTGAGAGATC AAATGACAGA TCTGAAACGA CAGCAGAGGA TAAACATGGA ACACGTGAAC 
GACATTGTGA GCGAGAAATC CCGGGAACTG GCTCAGTTTA TCGCTGTCAC AGCAGGCGCC 
CAGCACCGAC CGTTGCGTAT ACGAAGATTC CGACTTGACC AGCAGAGACA CGCTTACTCC 
GAGGATGCTA CGACCACGGA ACGAATGCTG TCGGAGGTGG AAGCCGCCGT AGAAGAACTA 
AGGAATGAAG TCGTGGAAAG AAGGTGCAGA GTTAACGCTC GTGAAGTCGA AGCTCTTGTT 
TTGCAACTCA GTCAAGCTGG AAGGACGGTA GCAAAGCTGA AAGGTTCATA TCCTGCTCTA 
TGTGAAGAGA TACGAACCGT AGGAGCAGGG GAAACGGAAG TCATTTACGC TGAAGAGAAG 
TTCGTTGAAG CGGAACCGAC ACGGTTGGAT GAACAGCTGA AACGATGTCG GCAACTTACT 
GCAACACTTT TCACTCTGAA AAAGTTGGCT ACAGTTCAAG AAGCGCGCAG TAAAACTCCG 
ACTTCATTCG GGGCAATTCG TCCGAACACA CCGAGTCAAC TCGGGGCTCG AAGAGGTGAA 
GATCCGAATG CGAGCCGAGG AAGAATGCTC GAGGAAGTTC GGAGGACTCG GGTCGATCCT 
GTAAGAAGAA TGGAGAGCAT TCGGGCACTG GAGTTGGCTG AGAAGGCACT TAATCGAACC 
AACCATCTTT CACTGATCGA CCAACGTCGT CATAACCACT TAATGGACCT GTACAAGAAT 
CGTGACGTCA TGAACCACAC ACCACGTAGC AATCTCACAA CTTCGGATTC GAGTTCCATT 
GAATCGTTAC CCCCTCCTCC GCCCCCTATG GATCATATGG GTCATCAATT CCACCCTAAT 
CAACAACCGA GCTATCCATT ACACCTCCCC CCTCCCCCAG CAGACCTTAA CCTTCCCGGC 
CCACCACCAC ACGAGTATTC TGTCATCTCC CCCGACATCG GGTATATGAG CTCGGATCTC 
TCCCCGGAGC ATCGTGGCCA AACACAGACC GGCTCACTAC CGAGGTATTC AAACGCCACG 
CGGTTTCATG GGTATCAGCA GCTTGAACGA AGCCATGGCA CCAGCCACCC TGCTTATGTG 
GATTACCACA ATCATGGCCG CCAGACGCCA CAGATGGTTG AAAGGTCACG ATCGGTGACG 
CCACACCACT GGAACCAGCG TCCCATCACG CCACAGATGT TTACAAGCGA GATACCAGTC 
GGACGCGAGT TGACAGAAGA TCAGCGACGT CATATATTGG AGCAACAACA TCTAATGAAC 
GAAGAGAAGA ATTGCAAGTT AAAAGAACAA TATTCAAAGC TGCAACAACT ACAACGGAAG 
CAGAGAATGG CAAGCAAGAA ATATGTACCG AAGCCGAACT ATCAGTCTAT GTCGATGGCG 
GCTTCAGCAA AATCGTCCGA TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026691608.1
CDS	16..5442
Translation	MDENTQEAGDSEVENDKASYAMLRPLQKVIAHFEMKLAANKGNIPTPRREIPNLKPQAERLRQSRKTSKQRSFIKSSGKLLSSVQEKVTVGSKSSTPCHSRSSSVCSSNVESHVPTSEGPPMTSSQQDFDTTSVSSTSSMTSETVDSQVKYSSLQRNYQHGHKSFQFNTLDGRRTRSKSGGHHNRSFYPQDGSISPAEADRKRSAFFQYLVQKYPQHSDVIVGKSFQQTQTPAPSFGGIRTRASYAADCSYNSSSPNDSEVTWHNSLSRGSRLRSSLPIVRSLSRSRERPLGTIYLEYSGECRTARLPNEVTSLDTIRALFVQAYPGLLTMPMLDPQRHSILLKNRKTGNYDEIDNVSRIKDRSFIRTRPIGGSGIPLPGTKKPTINHPKPAAKPVKPIPGNNRSKPRVTDDVKIAERNQKNLNKSGIKAPVKSLIRQYQSAPSLIKQAVTGSNEIKPVQSGEETQGKVKPPPPPRSAKPTVVAQTEAAQDQGNYSPNGTLLSGNGIGGGSIPQKTFSYVKSGNESPVNHQQQTITSPPQHRGLRQPTTSRLSPPSVEKKFSSIPTPGSKPKIQLPPNPTKPTDDEHHTKENVPRPKTPNILHQSPPTVTKLPPTSFAMTSPKNGAQRLPQPKPFGMKQHTTPANRFGAKRTSPPNVPNNNQLVPETAPVYSNIKRSASPERRATINGTGGSAGYCRGGRSFKGHQLKPVNIPSVPDTEIAAASNLEAKPDLNVPEVAQPVEPRQPISLPSDTKYSAMLPNPCTTDQVTSCSSSTSSDDVTDPGKSKMSTKKGLFGLRRLVSNRNKNPQNIPKRSQSEERPKTSDPSVNKSDLHSPSFNESLASFRSSSPALQVSTPVVRSSPIMSKSPAIPKSDLLNSLLTTNKETPEPNTDVMSTSSSDDDKDKPAPHPILAGVLKNNNLVKSTSSSSILSDSGASNVSMTSSIRTKKVSFQDDVTVCDGNGISTFSNGLREPDASVADVSFDRSMEQSLSVDTHEGAENEPKQWRPPSNGPPARNDFQQVGYRARPATPHRYEPPPYQPSPLAYQTNSPRSHQSAVAAAIQSISGNAETTSSAARYIPSQPEHFSVSCASPVPVVTRQGHEAPHKEEWNPLRRQSADASSNSPSITQRTCPTPVPTAPHKLAEVQVENEPSYFQTERAPSRSETFNSDTPPYYECCETTTPASGSITPSQLRDALKTQDEQYQHPVVESAGPENQSSVTFSSLPLSVPPSGNVLPQPETDVTNKKVMAMERQLASLTGMVESIITSTDGVQTNKPEPPYQNQPVGGNLPKTSHTVQQVMTSSEMGQQLLKLRETAHNLRDQMTDLKRQQRINMEHVNDIVSEKSRELAQFIAVTAGAQHRPLRIRRFRLDQQRHAYSEDATTTERMLSEVEAAVEELRNEVVERRCRVNAREVEALVLQLSQAGRTVAKLKGSYPALCEEIRTVGAGETEVIYAEEKFVEAEPTRLDEQLKRCRQLTATLFTLKKLATVQEARSKTPTSFGAIRPNTPSQLGARRGEDPNASRGRMLEEVRRTRVDPVRRMESIRALELAEKALNRTNHLSLIDQRRHNHLMDLYKNRDVMNHTPRSNLTTSDSSSIESLPPPPPPMDHMGHQFHPNQQPSYPLHLPPPPADLNLPGPPPHEYSVISPDIGYMSSDLSPEHRGQTQTGSLPRYSNATRFHGYQQLERSHGTSHPAYVDYHNHGRQTPQMVERSRSVTPHHWNQRPITPQMFTSEIPVGRELTEDQRRHILEQQHLMNEEKNCKLKEQYSKLQQLQRKQRMASKKYVPKPNYQSMSMAASAKSSDV

Target ORF information:

RefSeq Version	XM_026835807.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis coiled-coil domain-containing protein CG32809 (LOC100185798), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026835807.1

ORF Insert Sequence:

ATGGATGAAA ATACACAAGA AGCTGGCGAC TCAGAAGTCG AAAATGACAA GGCAAGTTAC 
GCGATGTTGA GACCCTTGCA GAAAGTGATC GCTCACTTTG AGATGAAACT TGCCGCCAAC 
AAAGGGAACA TTCCAACACC CAGACGAGAA ATACCAAACC TGAAACCGCA AGCTGAGCGT 
CTTCGTCAGA GCAGGAAAAC GTCCAAACAA CGAAGTTTTA TTAAAAGCAG TGGGAAGCTA 
TTGTCAAGCG TGCAAGAGAA GGTTACTGTT GGATCTAAAT CGTCAACACC TTGTCATAGC 
AGATCAAGTT CGGTGTGTTC AAGTAATGTG GAAAGTCATG TACCTACGTC AGAAGGCCCT 
CCCATGACAT CATCGCAACA AGATTTTGAC ACCACTTCTG TTTCGTCTAC GTCCAGTATG 
ACGTCAGAAA CTGTAGATTC TCAAGTTAAA TATTCCTCGT TACAAAGAAA CTACCAACAT 
GGGCACAAGA GTTTTCAGTT CAACACTCTG GATGGAAGGA GAACTCGATC TAAGAGTGGA 
GGCCACCATA ATCGAAGTTT CTATCCGCAA GATGGCAGTA TATCCCCAGC CGAAGCAGAT 
CGAAAGCGCT CAGCCTTTTT TCAATATTTA GTCCAAAAAT ACCCGCAACA CTCCGATGTT 
ATTGTTGGGA AATCATTTCA GCAAACCCAG ACTCCAGCAC CTTCCTTCGG TGGGATTCGA 
ACCCGTGCAA GCTACGCTGC CGATTGCTCT TATAACTCTT CATCTCCTAA CGACAGCGAG 
GTCACGTGGC ATAACAGTTT GTCACGTGGT AGTAGACTCA GGTCCAGCCT CCCAATCGTA 
AGAAGTTTAA GTCGAAGCCG AGAACGACCA TTAGGCACCA TTTACTTGGA GTATAGCGGA 
GAGTGTAGAA CTGCCAGACT GCCGAACGAA GTGACAAGCC TTGATACAAT ACGAGCTCTC 
TTCGTACAAG CATACCCAGG ATTGCTAACG ATGCCAATGC TTGACCCACA ACGTCACTCC 
ATTTTATTAA AAAACAGGAA GACGGGAAAC TACGATGAGA TCGATAACGT GAGTCGCATC 
AAGGATCGTT CATTCATTCG AACTCGTCCG ATCGGGGGTT CAGGTATTCC ATTGCCTGGG 
ACCAAGAAGC CTACAATCAA CCACCCGAAG CCTGCTGCTA AACCAGTTAA GCCAATACCG 
GGCAACAACC GATCAAAACC ACGGGTTACT GATGATGTAA AAATTGCAGA ACGCAACCAA 
AAGAACTTGA ACAAAAGTGG GATAAAAGCA CCGGTTAAAT CCCTGATCCG TCAATACCAG 
TCTGCACCTA GTTTAATAAA GCAAGCAGTG ACCGGTTCAA ACGAGATAAA ACCTGTTCAA 
AGTGGCGAGG AGACTCAGGG TAAAGTGAAA CCGCCTCCCC CACCGCGGTC CGCAAAGCCA 
ACAGTCGTAG CACAAACAGA AGCAGCCCAA GATCAAGGCA ATTATTCGCC TAACGGGACT 
CTATTATCAG GCAATGGGAT TGGGGGTGGT AGTATACCAC AGAAAACTTT CTCGTACGTT 
AAAAGCGGCA ATGAGTCTCC AGTAAACCAC CAACAACAGA CAATCACATC CCCACCGCAG 
CATAGGGGCC TGAGGCAACC TACAACTTCC AGGCTTTCCC CTCCATCTGT TGAGAAGAAG 
TTTTCCTCCA TACCAACGCC AGGGTCAAAG CCGAAGATCC AACTCCCTCC GAATCCAACA 
AAACCAACCG ATGACGAACA CCACACTAAG GAGAACGTGC CACGCCCAAA GACTCCCAAC 
ATACTCCACC AATCACCGCC CACCGTCACC AAACTTCCGC CTACGTCATT CGCAATGACG 
TCACCGAAAA ATGGCGCTCA ACGCCTTCCC CAACCAAAAC CTTTCGGCAT GAAACAACAT 
ACAACGCCAG CGAACCGGTT CGGTGCAAAA CGAACGTCCC CTCCAAACGT CCCAAACAAT 
AATCAATTGG TGCCGGAAAC TGCGCCGGTC TATTCAAATA TAAAGCGGTC AGCCTCACCG 
GAAAGAAGAG CTACAATAAA CGGTACCGGT GGATCAGCAG GTTACTGCAG GGGTGGTAGG 
TCCTTTAAAG GCCACCAGCT GAAACCTGTA AATATTCCAT CCGTTCCCGA CACTGAAATA 
GCTGCAGCTT CTAACCTGGA AGCCAAACCT GATCTCAACG TGCCGGAGGT AGCTCAACCT 
GTAGAACCGC GGCAACCCAT TTCATTACCC AGTGATACCA AATACTCTGC CATGCTACCG 
AACCCTTGTA CCACAGATCA AGTGACGTCA TGCAGCTCCT CTACGTCATC CGATGACGTC 
ACCGACCCTG GAAAATCAAA GATGTCAACG AAGAAGGGAT TGTTCGGGCT TCGGAGATTG 
GTTTCGAACC GAAACAAGAA CCCACAAAAT ATTCCGAAAC GAAGTCAAAG CGAGGAGCGA 
CCGAAGACAT CGGATCCTTC GGTAAATAAA TCCGACCTTC ATTCTCCATC TTTCAACGAG 
TCCTTGGCTT CGTTTAGGTC ATCGTCCCCG GCTCTTCAAG TCAGCACGCC AGTGGTTCGA 
TCTTCACCCA TTATGAGCAA GTCTCCTGCC ATACCGAAAA GTGATCTTCT AAATAGTTTG 
CTTACAACCA ACAAGGAGAC ACCTGAGCCG AACACAGATG TTATGTCCAC TTCCAGCTCG 
GATGACGACA AGGACAAACC TGCTCCTCAC CCCATACTGG CTGGAGTGCT TAAAAACAAC 
AACTTGGTCA AAAGCACTTC TTCCAGTTCG ATTTTGTCCG ACTCCGGCGC AAGTAACGTG 
TCTATGACGT CATCCATACG TACAAAGAAG GTTTCATTTC AAGACGACGT AACAGTTTGT 
GATGGTAATG GGATCAGTAC GTTTAGTAAC GGATTGCGAG AACCTGACGC TTCTGTAGCT 
GATGTATCGT TCGATAGGTC GATGGAACAA TCGTTATCTG TCGACACGCA TGAGGGTGCG 
GAGAATGAGC CAAAGCAGTG GCGACCGCCC TCTAACGGTC CGCCTGCTCG AAATGATTTC 
CAACAGGTGG GCTACAGAGC AAGACCCGCA ACACCACACA GGTATGAACC ACCACCATAC 
CAACCCTCCC CTCTTGCATA CCAAACCAAC TCTCCTCGAT CGCACCAATC AGCTGTGGCC 
GCTGCTATAC AAAGCATAAG TGGGAATGCG GAAACAACAA GCAGTGCTGC AAGGTACATT 
CCAAGCCAAC CTGAACATTT CTCGGTATCT TGCGCATCAC CTGTGCCAGT AGTAACCAGG 
CAGGGGCACG AGGCTCCACA CAAGGAGGAA TGGAATCCTC TCCGACGACA ATCCGCAGAC 
GCAAGCTCTA ACTCACCTTC AATTACACAG CGGACATGTC CTACCCCTGT GCCAACCGCC 
CCGCATAAGT TAGCTGAGGT ACAAGTAGAA AACGAGCCTT CATATTTCCA AACAGAGCGA 
GCGCCATCTC GTTCTGAAAC ATTTAACTCC GACACACCCC CCTACTACGA ATGCTGCGAA 
ACTACGACGC CCGCCTCTGG CTCAATTACA CCAAGTCAAT TAAGAGATGC GTTAAAGACA 
CAGGACGAAC AGTATCAACA TCCAGTCGTT GAATCTGCGG GCCCTGAGAA TCAAAGTTCT 
GTCACATTTT CCTCTCTCCC TTTATCTGTC CCACCATCAG GGAACGTTTT GCCACAACCC 
GAAACCGATG TTACGAACAA GAAGGTCATG GCTATGGAGC GCCAACTAGC GAGTTTAACC 
GGCATGGTTG AATCCATTAT AACCAGTACC GACGGTGTTC AAACCAACAA GCCTGAACCC 
CCATACCAGA ATCAACCAGT CGGTGGGAAT CTCCCTAAAA CGTCACACAC CGTCCAACAA 
GTGATGACGT CATCAGAGAT GGGACAACAA CTCTTGAAGT TGAGGGAAAC AGCTCATAAT 
CTGAGAGATC AAATGACAGA TCTGAAACGA CAGCAGAGGA TAAACATGGA ACACGTGAAC 
GACATTGTGA GCGAGAAATC CCGGGAACTG GCTCAGTTTA TCGCTGTCAC AGCAGGCGCC 
CAGCACCGAC CGTTGCGTAT ACGAAGATTC CGACTTGACC AGCAGAGACA CGCTTACTCC 
GAGGATGCTA CGACCACGGA ACGAATGCTG TCGGAGGTGG AAGCCGCCGT AGAAGAACTA 
AGGAATGAAG TCGTGGAAAG AAGGTGCAGA GTTAACGCTC GTGAAGTCGA AGCTCTTGTT 
TTGCAACTCA GTCAAGCTGG AAGGACGGTA GCAAAGCTGA AAGGTTCATA TCCTGCTCTA 
TGTGAAGAGA TACGAACCGT AGGAGCAGGG GAAACGGAAG TCATTTACGC TGAAGAGAAG 
TTCGTTGAAG CGGAACCGAC ACGGTTGGAT GAACAGCTGA AACGATGTCG GCAACTTACT 
GCAACACTTT TCACTCTGAA AAAGTTGGCT ACAGTTCAAG AAGCGCGCAG TAAAACTCCG 
ACTTCATTCG GGGCAATTCG TCCGAACACA CCGAGTCAAC TCGGGGCTCG AAGAGGTGAA 
GATCCGAATG CGAGCCGAGG AAGAATGCTC GAGGAAGTTC GGAGGACTCG GGTCGATCCT 
GTAAGAAGAA TGGAGAGCAT TCGGGCACTG GAGTTGGCTG AGAAGGCACT TAATCGAACC 
AACCATCTTT CACTGATCGA CCAACGTCGT CATAACCACT TAATGGACCT GTACAAGAAT 
CGTGACGTCA TGAACCACAC ACCACGTAGC AATCTCACAA CTTCGGATTC GAGTTCCATT 
GAATCGTTAC CCCCTCCTCC GCCCCCTATG GATCATATGG GTCATCAATT CCACCCTAAT 
CAACAACCGA GCTATCCATT ACACCTCCCC CCTCCCCCAG CAGACCTTAA CCTTCCCGGC 
CCACCACCAC ACGAGTATTC TGTCATCTCC CCCGACATCG GGTATATGAG CTCGGATCTC 
TCCCCGGAGC ATCGTGGCCA AACACAGACC GGCTCACTAC CGAGGTATTC AAACGCCACG 
CGGTTTCATG GGTATCAGCA GCTTGAACGA AGCCATGGCA CCAGCCACCC TGCTTATGTG 
GATTACCACA ATCATGGCCG CCAGACGCCA CAGATGGTTG AAAGGTCACG ATCGGTGACG 
CCACACCACT GGAACCAGCG TCCCATCACG CCACAGATGT TTACAAGCGA GATACCAGTC 
GGACGCGAGT TGACAGAAGA TCAGCGACGT CATATATTGG AGCAACAACA TCTAATGAAC 
GAAGAGAAGA ATTGCAAGTT AAAAGAACAA TATTCAAAGC TGCAACAACT ACAACGGAAG 
CAGAGAATGG CAAGCAAGAA ATATGTACCG AAGCCGAACT ATCAGTCTAT GTCGATGGCG 
GCTTCAGCAA AATCGTCCGA TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100185798 ( XM_026835808.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_026691609.1 Ciona intestinalis coiled-coil domain-containing protein CG32809 (LOC100185798), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCi156735
Clone ID Related Accession (Same CDS sequence)	XM_026835808.1
Accession Version	XM_026835808.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1540051200000
Organism	Ciona intestinalis(vase tunicate)
Product	coiled-coil domain-containing protein CG32809 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020174.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ciona intestinalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTTG ACACCACTTC TGTTTCGTCT ACGTCCAGTA TGACGTCAGA AACTGTAGAT 
TCTCAAGTTA AATATTCCTC GTTACAAAGA AACTACCAAC ATGGGCACAA GAGTTTTCAG 
TTCAACACTC TGGATGGAAG GAGAACTCGA TCTAAGAGTG GAGGCCACCA TAATCGAAGT 
TTCTATCCGC AAGATGGCAG TATATCCCCA GCCGAAGCAG ATCGAAAGCG CTCAGCCTTT 
TTTCAATATT TAGTCCAAAA ATACCCGCAA CACTCCGATG TTATTGTTGG GAAATCATTT 
CAGCAAACCC AGACTCCAGC ACCTTCCTTC GGTGGGATTC GAACCCGTGC AAGCTACGCT 
GCCGATTGCT CTTATAACTC TTCATCTCCT AACGACAGCG AGGTCACGTG GCATAACAGT 
TTGTCACGTG GTAGTAGACT CAGGTCCAGC CTCCCAATCG TAAGAAGTTT AAGTCGAAGC 
CGAGAACGAC CATTAGGCAC CATTTACTTG GAGTATAGCG GAGAGTGTAG AACTGCCAGA 
CTGCCGAACG AAGTGACAAG CCTTGATACA ATACGAGCTC TCTTCGTACA AGCATACCCA 
GGATTGCTAA CGATGCCAAT GCTTGACCCA CAACGTCACT CCATTTTATT AAAAAACAGG 
AAGACGGGAA ACTACGATGA GATCGATAAC GTGAGTCGCA TCAAGGATCG TTCATTCATT 
CGAACTCGTC CGATCGGGGG TTCAGGTATT CCATTGCCTG GGACCAAGAA GCCTACAATC 
AACCACCCGA AGCCTGCTGC TAAACCAGTT AAGCCAATAC CGGGCAACAA CCGATCAAAA 
CCACGGGTTA CTGATGATGT AAAAATTGCA GAACGCAACC AAAAGAACTT GAACAAAAGT 
GGGATAAAAG CACCGGTTAA ATCCCTGATC CGTCAATACC AGTCTGCACC TAGTTTAATA 
AAGCAAGCAG TGACCGGTTC AAACGAGATA AAACCTGTTC AAAGTGGCGA GGAGACTCAG 
GGTAAAGTGA AACCGCCTCC CCCACCGCGG TCCGCAAAGC CAACAGTCGT AGCACAAACA 
GAAGCAGCCC AAGATCAAGG CAATTATTCG CCTAACGGGA CTCTATTATC AGGCAATGGG 
ATTGGGGGTG GTAGTATACC ACAGAAAACT TTCTCGTACG TTAAAAGCGG CAATGAGTCT 
CCAGTAAACC ACCAACAACA GACAATCACA TCCCCACCGC AGCATAGGGG CCTGAGGCAA 
CCTACAACTT CCAGGCTTTC CCCTCCATCT GTTGAGAAGA AGTTTTCCTC CATACCAACG 
CCAGGGTCAA AGCCGAAGAT CCAACTCCCT CCGAATCCAA CAAAACCAAC CGATGACGAA 
CACCACACTA AGGAGAACGT GCCACGCCCA AAGACTCCCA ACATACTCCA CCAATCACCG 
CCCACCGTCA CCAAACTTCC GCCTACGTCA TTCGCAATGA CGTCACCGAA AAATGGCGCT 
CAACGCCTTC CCCAACCAAA ACCTTTCGGC ATGAAACAAC ATACAACGCC AGCGAACCGG 
TTCGGTGCAA AACGAACGTC CCCTCCAAAC GTCCCAAACA ATAATCAATT GGTGCCGGAA 
ACTGCGCCGG TCTATTCAAA TATAAAGCGG TCAGCCTCAC CGGAAAGAAG AGCTACAATA 
AACGGTACCG GTGGATCAGC AGGTTACTGC AGGGGTGGTA GGTCCTTTAA AGGCCACCAG 
CTGAAACCTG TAAATATTCC ATCCGTTCCC GACACTGAAA TAGCTGCAGC TTCTAACCTG 
GAAGCCAAAC CTGATCTCAA CGTGCCGGAG GTAGCTCAAC CTGTAGAACC GCGGCAACCC 
ATTTCATTAC CCAGTGATAC CAAATACTCT GCCATGCTAC CGAACCCTTG TACCACAGAT 
CAAGTGACGT CATGCAGCTC CTCTACGTCA TCCGATGACG TCACCGACCC TGGAAAATCA 
AAGATGTCAA CGAAGAAGGG ATTGTTCGGG CTTCGGAGAT TGGTTTCGAA CCGAAACAAG 
AACCCACAAA ATATTCCGAA ACGAAGTCAA AGCGAGGAGC GACCGAAGAC ATCGGATCCT 
TCGGTAAATA AATCCGACCT TCATTCTCCA TCTTTCAACG AGTCCTTGGC TTCGTTTAGG 
TCATCGTCCC CGGCTCTTCA AGTCAGCACG CCAGTGGTTC GATCTTCACC CATTATGAGC 
AAGTCTCCTG CCATACCGAA AAGTGATCTT CTAAATAGTT TGCTTACAAC CAACAAGGAG 
ACACCTGAGC CGAACACAGA TGTTATGTCC ACTTCCAGCT CGGATGACGA CAAGGACAAA 
CCTGCTCCTC ACCCCATACT GGCTGGAGTG CTTAAAAACA ACAACTTGGT CAAAAGCACT 
TCTTCCAGTT CGATTTTGTC CGACTCCGGC GCAAGTAACG TGTCTATGAC GTCATCCATA 
CGTACAAAGA AGGTTTCATT TCAAGACGAC GTAACAGTTT GTGATGGTAA TGGGATCAGT 
ACGTTTAGTA ACGGATTGCG AGAACCTGAC GCTTCTGTAG CTGATGTATC GTTCGATAGG 
TCGATGGAAC AATCGTTATC TGTCGACACG CATGAGGGTG CGGAGAATGA GCCAAAGCAG 
TGGCGACCGC CCTCTAACGG TCCGCCTGCT CGAAATGATT TCCAACAGGT GGGCTACAGA 
GCAAGACCCG CAACACCACA CAGGTATGAA CCACCACCAT ACCAACCCTC CCCTCTTGCA 
TACCAAACCA ACTCTCCTCG ATCGCACCAA TCAGCTGTGG CCGCTGCTAT ACAAAGCATA 
AGTGGGAATG CGGAAACAAC AAGCAGTGCT GCAAGGTACA TTCCAAGCCA ACCTGAACAT 
TTCTCGGTAT CTTGCGCATC ACCTGTGCCA GTAGTAACCA GGCAGGGGCA CGAGGCTCCA 
CACAAGGAGG AATGGAATCC TCTCCGACGA CAATCCGCAG ACGCAAGCTC TAACTCACCT 
TCAATTACAC AGCGGACATG TCCTACCCCT GTGCCAACCG CCCCGCATAA GTTAGCTGAG 
GTACAAGTAG AAAACGAGCC TTCATATTTC CAAACAGAGC GAGCGCCATC TCGTTCTGAA 
ACATTTAACT CCGACACACC CCCCTACTAC GAATGCTGCG AAACTACGAC GCCCGCCTCT 
GGCTCAATTA CACCAAGTCA ATTAAGAGAT GCGTTAAAGA CACAGGACGA ACAGTATCAA 
CATCCAGTCG TTGAATCTGC GGGCCCTGAG AATCAAAGTT CTGTCACATT TTCCTCTCTC 
CCTTTATCTG TCCCACCATC AGGGAACGTT TTGCCACAAC CCGAAACCGA TGTTACGAAC 
AAGAAGGTCA TGGCTATGGA GCGCCAACTA GCGAGTTTAA CCGGCATGGT TGAATCCATT 
ATAACCAGTA CCGACGGTGT TCAAACCAAC AAGCCTGAAC CCCCATACCA GAATCAACCA 
GTCGGTGGGA ATCTCCCTAA AACGTCACAC ACCGTCCAAC AAGTGATGAC GTCATCAGAG 
ATGGGACAAC AACTCTTGAA GTTGAGGGAA ACAGCTCATA ATCTGAGAGA TCAAATGACA 
GATCTGAAAC GACAGCAGAG GATAAACATG GAACACGTGA ACGACATTGT GAGCGAGAAA 
TCCCGGGAAC TGGCTCAGTT TATCGCTGTC ACAGCAGGCG CCCAGCACCG ACCGTTGCGT 
ATACGAAGAT TCCGACTTGA CCAGCAGAGA CACGCTTACT CCGAGGATGC TACGACCACG 
GAACGAATGC TGTCGGAGGT GGAAGCCGCC GTAGAAGAAC TAAGGAATGA AGTCGTGGAA 
AGAAGGTGCA GAGTTAACGC TCGTGAAGTC GAAGCTCTTG TTTTGCAACT CAGTCAAGCT 
GGAAGGACGG TAGCAAAGCT GAAAGGTTCA TATCCTGCTC TATGTGAAGA GATACGAACC 
GTAGGAGCAG GGGAAACGGA AGTCATTTAC GCTGAAGAGA AGTTCGTTGA AGCGGAACCG 
ACACGGTTGG ATGAACAGCT GAAACGATGT CGGCAACTTA CTGCAACACT TTTCACTCTG 
AAAAAGTTGG CTACAGTTCA AGAAGCGCGC AGTAAAACTC CGACTTCATT CGGGGCAATT 
CGTCCGAACA CACCGAGTCA ACTCGGGGCT CGAAGAGGTG AAGATCCGAA TGCGAGCCGA 
GGAAGAATGC TCGAGGAAGT TCGGAGGACT CGGGTCGATC CTGTAAGAAG AATGGAGAGC 
ATTCGGGATG CTGAGGTTAG TTGGATACGA AGGCGAAAGT ACAACAGTGA ATACGACTCG 
AAAAAATTCG AGAAGGCACT GGAGTTGGCT GAGAAGGCAC TTAATCGAAC CAACCATCTT 
TCACTGATCG ACCAACGTCG TCATAACCAC TTAATGGACC TGTACAAGAA TCGTGACGTC 
ATGAACCACA CACCACGTAG CAATCTCACA ACTTCGGATT CGAGTTCCAT TGAATCGTTA 
CCCCCTCCTC CGCCCCCTAT GGATCATATG GGTCATCAAT TCCACCCTAA TCAACAACCG 
AGCTATCCAT TACACCTCCC CCCTCCCCCA GCAGACCTTA ACCTTCCCGG CCCACCACCA 
CACGAGTATT CTGTCATCTC CCCCGACATC GGGTATATGA GCTCGGATCT CTCCCCGGAG 
CATCGTGGCC AAACACAGAC CGGCTCACTA CCGAGGTATT CAAACGCCAC GCGGTTTCAT 
GGGTATCAGC AGCTTGAACG AAGCCATGGC ACCAGCCACC CTGCTTATGT GGATTACCAC 
AATCATGGCC GCCAGACGCC ACAGATGGTT GAAAGGTCAC GATCGGTGAC GCCACACCAC 
TGGAACCAGC GTCCCATCAC GCCACAGATG TTTACAAGCG AGATACCAGT CGGACGCGAG 
TTGACAGAAG ATCAGCGACG TCATATATTG GAGCAACAAC ATCTAATGAA CGAAGAGAAG 
AATTGCAAGT TAAAAGAACA ATATTCAAAG CTGCAACAAC TACAACGGAA GCAGAGAATG 
GCAAGCAAGA AATATGTACC GAAGCCGAAC TATCAGTCTA TGTCGATGGC GGCTTCAGCA 
AAATCGTCCG ATGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026691609.1
CDS	140..5257
Translation	MDFDTTSVSSTSSMTSETVDSQVKYSSLQRNYQHGHKSFQFNTLDGRRTRSKSGGHHNRSFYPQDGSISPAEADRKRSAFFQYLVQKYPQHSDVIVGKSFQQTQTPAPSFGGIRTRASYAADCSYNSSSPNDSEVTWHNSLSRGSRLRSSLPIVRSLSRSRERPLGTIYLEYSGECRTARLPNEVTSLDTIRALFVQAYPGLLTMPMLDPQRHSILLKNRKTGNYDEIDNVSRIKDRSFIRTRPIGGSGIPLPGTKKPTINHPKPAAKPVKPIPGNNRSKPRVTDDVKIAERNQKNLNKSGIKAPVKSLIRQYQSAPSLIKQAVTGSNEIKPVQSGEETQGKVKPPPPPRSAKPTVVAQTEAAQDQGNYSPNGTLLSGNGIGGGSIPQKTFSYVKSGNESPVNHQQQTITSPPQHRGLRQPTTSRLSPPSVEKKFSSIPTPGSKPKIQLPPNPTKPTDDEHHTKENVPRPKTPNILHQSPPTVTKLPPTSFAMTSPKNGAQRLPQPKPFGMKQHTTPANRFGAKRTSPPNVPNNNQLVPETAPVYSNIKRSASPERRATINGTGGSAGYCRGGRSFKGHQLKPVNIPSVPDTEIAAASNLEAKPDLNVPEVAQPVEPRQPISLPSDTKYSAMLPNPCTTDQVTSCSSSTSSDDVTDPGKSKMSTKKGLFGLRRLVSNRNKNPQNIPKRSQSEERPKTSDPSVNKSDLHSPSFNESLASFRSSSPALQVSTPVVRSSPIMSKSPAIPKSDLLNSLLTTNKETPEPNTDVMSTSSSDDDKDKPAPHPILAGVLKNNNLVKSTSSSSILSDSGASNVSMTSSIRTKKVSFQDDVTVCDGNGISTFSNGLREPDASVADVSFDRSMEQSLSVDTHEGAENEPKQWRPPSNGPPARNDFQQVGYRARPATPHRYEPPPYQPSPLAYQTNSPRSHQSAVAAAIQSISGNAETTSSAARYIPSQPEHFSVSCASPVPVVTRQGHEAPHKEEWNPLRRQSADASSNSPSITQRTCPTPVPTAPHKLAEVQVENEPSYFQTERAPSRSETFNSDTPPYYECCETTTPASGSITPSQLRDALKTQDEQYQHPVVESAGPENQSSVTFSSLPLSVPPSGNVLPQPETDVTNKKVMAMERQLASLTGMVESIITSTDGVQTNKPEPPYQNQPVGGNLPKTSHTVQQVMTSSEMGQQLLKLRETAHNLRDQMTDLKRQQRINMEHVNDIVSEKSRELAQFIAVTAGAQHRPLRIRRFRLDQQRHAYSEDATTTERMLSEVEAAVEELRNEVVERRCRVNAREVEALVLQLSQAGRTVAKLKGSYPALCEEIRTVGAGETEVIYAEEKFVEAEPTRLDEQLKRCRQLTATLFTLKKLATVQEARSKTPTSFGAIRPNTPSQLGARRGEDPNASRGRMLEEVRRTRVDPVRRMESIRDAEVSWIRRRKYNSEYDSKKFEKALELAEKALNRTNHLSLIDQRRHNHLMDLYKNRDVMNHTPRSNLTTSDSSSIESLPPPPPPMDHMGHQFHPNQQPSYPLHLPPPPADLNLPGPPPHEYSVISPDIGYMSSDLSPEHRGQTQTGSLPRYSNATRFHGYQQLERSHGTSHPAYVDYHNHGRQTPQMVERSRSVTPHHWNQRPITPQMFTSEIPVGRELTEDQRRHILEQQHLMNEEKNCKLKEQYSKLQQLQRKQRMASKKYVPKPNYQSMSMAASAKSSDV

Target ORF information:

RefSeq Version	XM_026835808.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis coiled-coil domain-containing protein CG32809 (LOC100185798), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026835808.1

ORF Insert Sequence:

ATGGATTTTG ACACCACTTC TGTTTCGTCT ACGTCCAGTA TGACGTCAGA AACTGTAGAT 
TCTCAAGTTA AATATTCCTC GTTACAAAGA AACTACCAAC ATGGGCACAA GAGTTTTCAG 
TTCAACACTC TGGATGGAAG GAGAACTCGA TCTAAGAGTG GAGGCCACCA TAATCGAAGT 
TTCTATCCGC AAGATGGCAG TATATCCCCA GCCGAAGCAG ATCGAAAGCG CTCAGCCTTT 
TTTCAATATT TAGTCCAAAA ATACCCGCAA CACTCCGATG TTATTGTTGG GAAATCATTT 
CAGCAAACCC AGACTCCAGC ACCTTCCTTC GGTGGGATTC GAACCCGTGC AAGCTACGCT 
GCCGATTGCT CTTATAACTC TTCATCTCCT AACGACAGCG AGGTCACGTG GCATAACAGT 
TTGTCACGTG GTAGTAGACT CAGGTCCAGC CTCCCAATCG TAAGAAGTTT AAGTCGAAGC 
CGAGAACGAC CATTAGGCAC CATTTACTTG GAGTATAGCG GAGAGTGTAG AACTGCCAGA 
CTGCCGAACG AAGTGACAAG CCTTGATACA ATACGAGCTC TCTTCGTACA AGCATACCCA 
GGATTGCTAA CGATGCCAAT GCTTGACCCA CAACGTCACT CCATTTTATT AAAAAACAGG 
AAGACGGGAA ACTACGATGA GATCGATAAC GTGAGTCGCA TCAAGGATCG TTCATTCATT 
CGAACTCGTC CGATCGGGGG TTCAGGTATT CCATTGCCTG GGACCAAGAA GCCTACAATC 
AACCACCCGA AGCCTGCTGC TAAACCAGTT AAGCCAATAC CGGGCAACAA CCGATCAAAA 
CCACGGGTTA CTGATGATGT AAAAATTGCA GAACGCAACC AAAAGAACTT GAACAAAAGT 
GGGATAAAAG CACCGGTTAA ATCCCTGATC CGTCAATACC AGTCTGCACC TAGTTTAATA 
AAGCAAGCAG TGACCGGTTC AAACGAGATA AAACCTGTTC AAAGTGGCGA GGAGACTCAG 
GGTAAAGTGA AACCGCCTCC CCCACCGCGG TCCGCAAAGC CAACAGTCGT AGCACAAACA 
GAAGCAGCCC AAGATCAAGG CAATTATTCG CCTAACGGGA CTCTATTATC AGGCAATGGG 
ATTGGGGGTG GTAGTATACC ACAGAAAACT TTCTCGTACG TTAAAAGCGG CAATGAGTCT 
CCAGTAAACC ACCAACAACA GACAATCACA TCCCCACCGC AGCATAGGGG CCTGAGGCAA 
CCTACAACTT CCAGGCTTTC CCCTCCATCT GTTGAGAAGA AGTTTTCCTC CATACCAACG 
CCAGGGTCAA AGCCGAAGAT CCAACTCCCT CCGAATCCAA CAAAACCAAC CGATGACGAA 
CACCACACTA AGGAGAACGT GCCACGCCCA AAGACTCCCA ACATACTCCA CCAATCACCG 
CCCACCGTCA CCAAACTTCC GCCTACGTCA TTCGCAATGA CGTCACCGAA AAATGGCGCT 
CAACGCCTTC CCCAACCAAA ACCTTTCGGC ATGAAACAAC ATACAACGCC AGCGAACCGG 
TTCGGTGCAA AACGAACGTC CCCTCCAAAC GTCCCAAACA ATAATCAATT GGTGCCGGAA 
ACTGCGCCGG TCTATTCAAA TATAAAGCGG TCAGCCTCAC CGGAAAGAAG AGCTACAATA 
AACGGTACCG GTGGATCAGC AGGTTACTGC AGGGGTGGTA GGTCCTTTAA AGGCCACCAG 
CTGAAACCTG TAAATATTCC ATCCGTTCCC GACACTGAAA TAGCTGCAGC TTCTAACCTG 
GAAGCCAAAC CTGATCTCAA CGTGCCGGAG GTAGCTCAAC CTGTAGAACC GCGGCAACCC 
ATTTCATTAC CCAGTGATAC CAAATACTCT GCCATGCTAC CGAACCCTTG TACCACAGAT 
CAAGTGACGT CATGCAGCTC CTCTACGTCA TCCGATGACG TCACCGACCC TGGAAAATCA 
AAGATGTCAA CGAAGAAGGG ATTGTTCGGG CTTCGGAGAT TGGTTTCGAA CCGAAACAAG 
AACCCACAAA ATATTCCGAA ACGAAGTCAA AGCGAGGAGC GACCGAAGAC ATCGGATCCT 
TCGGTAAATA AATCCGACCT TCATTCTCCA TCTTTCAACG AGTCCTTGGC TTCGTTTAGG 
TCATCGTCCC CGGCTCTTCA AGTCAGCACG CCAGTGGTTC GATCTTCACC CATTATGAGC 
AAGTCTCCTG CCATACCGAA AAGTGATCTT CTAAATAGTT TGCTTACAAC CAACAAGGAG 
ACACCTGAGC CGAACACAGA TGTTATGTCC ACTTCCAGCT CGGATGACGA CAAGGACAAA 
CCTGCTCCTC ACCCCATACT GGCTGGAGTG CTTAAAAACA ACAACTTGGT CAAAAGCACT 
TCTTCCAGTT CGATTTTGTC CGACTCCGGC GCAAGTAACG TGTCTATGAC GTCATCCATA 
CGTACAAAGA AGGTTTCATT TCAAGACGAC GTAACAGTTT GTGATGGTAA TGGGATCAGT 
ACGTTTAGTA ACGGATTGCG AGAACCTGAC GCTTCTGTAG CTGATGTATC GTTCGATAGG 
TCGATGGAAC AATCGTTATC TGTCGACACG CATGAGGGTG CGGAGAATGA GCCAAAGCAG 
TGGCGACCGC CCTCTAACGG TCCGCCTGCT CGAAATGATT TCCAACAGGT GGGCTACAGA 
GCAAGACCCG CAACACCACA CAGGTATGAA CCACCACCAT ACCAACCCTC CCCTCTTGCA 
TACCAAACCA ACTCTCCTCG ATCGCACCAA TCAGCTGTGG CCGCTGCTAT ACAAAGCATA 
AGTGGGAATG CGGAAACAAC AAGCAGTGCT GCAAGGTACA TTCCAAGCCA ACCTGAACAT 
TTCTCGGTAT CTTGCGCATC ACCTGTGCCA GTAGTAACCA GGCAGGGGCA CGAGGCTCCA 
CACAAGGAGG AATGGAATCC TCTCCGACGA CAATCCGCAG ACGCAAGCTC TAACTCACCT 
TCAATTACAC AGCGGACATG TCCTACCCCT GTGCCAACCG CCCCGCATAA GTTAGCTGAG 
GTACAAGTAG AAAACGAGCC TTCATATTTC CAAACAGAGC GAGCGCCATC TCGTTCTGAA 
ACATTTAACT CCGACACACC CCCCTACTAC GAATGCTGCG AAACTACGAC GCCCGCCTCT 
GGCTCAATTA CACCAAGTCA ATTAAGAGAT GCGTTAAAGA CACAGGACGA ACAGTATCAA 
CATCCAGTCG TTGAATCTGC GGGCCCTGAG AATCAAAGTT CTGTCACATT TTCCTCTCTC 
CCTTTATCTG TCCCACCATC AGGGAACGTT TTGCCACAAC CCGAAACCGA TGTTACGAAC 
AAGAAGGTCA TGGCTATGGA GCGCCAACTA GCGAGTTTAA CCGGCATGGT TGAATCCATT 
ATAACCAGTA CCGACGGTGT TCAAACCAAC AAGCCTGAAC CCCCATACCA GAATCAACCA 
GTCGGTGGGA ATCTCCCTAA AACGTCACAC ACCGTCCAAC AAGTGATGAC GTCATCAGAG 
ATGGGACAAC AACTCTTGAA GTTGAGGGAA ACAGCTCATA ATCTGAGAGA TCAAATGACA 
GATCTGAAAC GACAGCAGAG GATAAACATG GAACACGTGA ACGACATTGT GAGCGAGAAA 
TCCCGGGAAC TGGCTCAGTT TATCGCTGTC ACAGCAGGCG CCCAGCACCG ACCGTTGCGT 
ATACGAAGAT TCCGACTTGA CCAGCAGAGA CACGCTTACT CCGAGGATGC TACGACCACG 
GAACGAATGC TGTCGGAGGT GGAAGCCGCC GTAGAAGAAC TAAGGAATGA AGTCGTGGAA 
AGAAGGTGCA GAGTTAACGC TCGTGAAGTC GAAGCTCTTG TTTTGCAACT CAGTCAAGCT 
GGAAGGACGG TAGCAAAGCT GAAAGGTTCA TATCCTGCTC TATGTGAAGA GATACGAACC 
GTAGGAGCAG GGGAAACGGA AGTCATTTAC GCTGAAGAGA AGTTCGTTGA AGCGGAACCG 
ACACGGTTGG ATGAACAGCT GAAACGATGT CGGCAACTTA CTGCAACACT TTTCACTCTG 
AAAAAGTTGG CTACAGTTCA AGAAGCGCGC AGTAAAACTC CGACTTCATT CGGGGCAATT 
CGTCCGAACA CACCGAGTCA ACTCGGGGCT CGAAGAGGTG AAGATCCGAA TGCGAGCCGA 
GGAAGAATGC TCGAGGAAGT TCGGAGGACT CGGGTCGATC CTGTAAGAAG AATGGAGAGC 
ATTCGGGATG CTGAGGTTAG TTGGATACGA AGGCGAAAGT ACAACAGTGA ATACGACTCG 
AAAAAATTCG AGAAGGCACT GGAGTTGGCT GAGAAGGCAC TTAATCGAAC CAACCATCTT 
TCACTGATCG ACCAACGTCG TCATAACCAC TTAATGGACC TGTACAAGAA TCGTGACGTC 
ATGAACCACA CACCACGTAG CAATCTCACA ACTTCGGATT CGAGTTCCAT TGAATCGTTA 
CCCCCTCCTC CGCCCCCTAT GGATCATATG GGTCATCAAT TCCACCCTAA TCAACAACCG 
AGCTATCCAT TACACCTCCC CCCTCCCCCA GCAGACCTTA ACCTTCCCGG CCCACCACCA 
CACGAGTATT CTGTCATCTC CCCCGACATC GGGTATATGA GCTCGGATCT CTCCCCGGAG 
CATCGTGGCC AAACACAGAC CGGCTCACTA CCGAGGTATT CAAACGCCAC GCGGTTTCAT 
GGGTATCAGC AGCTTGAACG AAGCCATGGC ACCAGCCACC CTGCTTATGT GGATTACCAC 
AATCATGGCC GCCAGACGCC ACAGATGGTT GAAAGGTCAC GATCGGTGAC GCCACACCAC 
TGGAACCAGC GTCCCATCAC GCCACAGATG TTTACAAGCG AGATACCAGT CGGACGCGAG 
TTGACAGAAG ATCAGCGACG TCATATATTG GAGCAACAAC ATCTAATGAA CGAAGAGAAG 
AATTGCAAGT TAAAAGAACA ATATTCAAAG CTGCAACAAC TACAACGGAA GCAGAGAATG 
GCAAGCAAGA AATATGTACC GAAGCCGAAC TATCAGTCTA TGTCGATGGC GGCTTCAGCA 
AAATCGTCCG ATGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100185798 ( XM_026835809.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_026691610.1 Ciona intestinalis coiled-coil domain-containing protein CG32809 (LOC100185798), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCi156736
Clone ID Related Accession (Same CDS sequence)	XM_026835809.1
Accession Version	XM_026835809.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1540051200000
Organism	Ciona intestinalis(vase tunicate)
Product	coiled-coil domain-containing protein CG32809 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020174.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ciona intestinalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAACAA AGAGCGAAAC TCTCCTCCTG CAGCCAGAGA GGGTCTGTTT AAATGGTGAT 
GATGTTAAAA AAGTTGATTG TAGCACCATT TACTTGGAGT ATAGCGGAGA GTGTAGAACT 
GCCAGACTGC CGAACGAAGT GACAAGCCTT GATACAATAC GAGCTCTCTT CGTACAAGCA 
TACCCAGGAT TGCTAACGAT GCCAATGCTT GACCCACAAC GTCACTCCAT TTTATTAAAA 
AACAGGAAGA CGGGAAACTA CGATGAGATC GATAACGTGA GTCGCATCAA GGATCGTTCA 
TTCATTCGAA CTCGTCCGAT CGGGGGTTCA GGTATTCCAT TGCCTGGGAC CAAGAAGCCT 
ACAATCAACC ACCCGAAGCC TGCTGCTAAA CCAGTTAAGC CAATACCGGG CAACAACCGA 
TCAAAACCAC GGGTTACTGA TGATGTAAAA ATTGCAGAAC GCAACCAAAA GAACTTGAAC 
AAAAGTGGGA TAAAAGCACC GGTTAAATCC CTGATCCGTC AATACCAGTC TGCACCTAGT 
TTAATAAAGC AAGCAGTGAC CGGTTCAAAC GAGATAAAAC CTGTTCAAAG TGGCGAGGAG 
ACTCAGGGTA AAGTGAAACC GCCTCCCCCA CCGCGGTCCG CAAAGCCAAC AGTCGTAGCA 
CAAACAGAAG CAGCCCAAGA TCAAGGCAAT TATTCGCCTA ACGGGACTCT ATTATCAGGC 
AATGGGATTG GGGGTGGTAG TATACCACAG AAAACTTTCT CGTACGTTAA AAGCGGCAAT 
GAGTCTCCAG TAAACCACCA ACAACAGACA ATCACATCCC CACCGCAGCA TAGGGGCCTG 
AGGCAACCTA CAACTTCCAG GCTTTCCCCT CCATCTGTTG AGAAGAAGTT TTCCTCCATA 
CCAACGCCAG GGTCAAAGCC GAAGATCCAA CTCCCTCCGA ATCCAACAAA ACCAACCGAT 
GACGAACACC ACACTAAGGA GAACGTGCCA CGCCCAAAGA CTCCCAACAT ACTCCACCAA 
TCACCGCCCA CCGTCACCAA ACTTCCGCCT ACGTCATTCG CAATGACGTC ACCGAAAAAT 
GGCGCTCAAC GCCTTCCCCA ACCAAAACCT TTCGGCATGA AACAACATAC AACGCCAGCG 
AACCGGTTCG GTGCAAAACG AACGTCCCCT CCAAACGTCC CAAACAATAA TCAATTGGTG 
CCGGAAACTG CGCCGGTCTA TTCAAATATA AAGCGGTCAG CCTCACCGGA AAGAAGAGCT 
ACAATAAACG GTACCGGTGG ATCAGCAGGT TACTGCAGGG GTGGTAGGTC CTTTAAAGGC 
CACCAGCTGA AACCTGTAAA TATTCCATCC GTTCCCGACA CTGAAATAGC TGCAGCTTCT 
AACCTGGAAG CCAAACCTGA TCTCAACGTG CCGGAGGTAG CTCAACCTGT AGAACCGCGG 
CAACCCATTT CATTACCCAG TGATACCAAA TACTCTGCCA TGCTACCGAA CCCTTGTACC 
ACAGATCAAG TGACGTCATG CAGCTCCTCT ACGTCATCCG ATGACGTCAC CGACCCTGGA 
AAATCAAAGA TGTCAACGAA GAAGGGATTG TTCGGGCTTC GGAGATTGGT TTCGAACCGA 
AACAAGAACC CACAAAATAT TCCGAAACGA AGTCAAAGCG AGGAGCGACC GAAGACATCG 
GATCCTTCGG TAAATAAATC CGACCTTCAT TCTCCATCTT TCAACGAGTC CTTGGCTTCG 
TTTAGGTCAT CGTCCCCGGC TCTTCAAGTC AGCACGCCAG TGGTTCGATC TTCACCCATT 
ATGAGCAAGT CTCCTGCCAT ACCGAAAAGT GATCTTCTAA ATAGTTTGCT TACAACCAAC 
AAGGAGACAC CTGAGCCGAA CACAGATGTT ATGTCCACTT CCAGCTCGGA TGACGACAAG 
GACAAACCTG CTCCTCACCC CATACTGGCT GGAGTGCTTA AAAACAACAA CTTGGTCAAA 
AGCACTTCTT CCAGTTCGAT TTTGTCCGAC TCCGGCGCAA GTAACGTGTC TATGACGTCA 
TCCATACGTA CAAAGAAGGT TTCATTTCAA GACGACGTAA CAGTTTGTGA TGGTAATGGG 
ATCAGTACGT TTAGTAACGG ATTGCGAGAA CCTGACGCTT CTGTAGCTGA TGTATCGTTC 
GATAGGTCGA TGGAACAATC GTTATCTGTC GACACGCATG AGGGTGCGGA GAATGAGCCA 
AAGCAGTGGC GACCGCCCTC TAACGGTCCG CCTGCTCGAA ATGATTTCCA ACAGGTGGGC 
TACAGAGCAA GACCCGCAAC ACCACACAGG TATGAACCAC CACCATACCA ACCCTCCCCT 
CTTGCATACC AAACCAACTC TCCTCGATCG CACCAATCAG CTGTGGCCGC TGCTATACAA 
AGCATAAGTG GGAATGCGGA AACAACAAGC AGTGCTGCAA GGTACATTCC AAGCCAACCT 
GAACATTTCT CGGTATCTTG CGCATCACCT GTGCCAGTAG TAACCAGGCA GGGGCACGAG 
GCTCCACACA AGGAGGAATG GAATCCTCTC CGACGACAAT CCGCAGACGC AAGCTCTAAC 
TCACCTTCAA TTACACAGCG GACATGTCCT ACCCCTGTGC CAACCGCCCC GCATAAGTTA 
GCTGAGGTAC AAGTAGAAAA CGAGCCTTCA TATTTCCAAA CAGAGCGAGC GCCATCTCGT 
TCTGAAACAT TTAACTCCGA CACACCCCCC TACTACGAAT GCTGCGAAAC TACGACGCCC 
GCCTCTGGCT CAATTACACC AAGTCAATTA AGAGATGCGT TAAAGACACA GGACGAACAG 
TATCAACATC CAGTCGTTGA ATCTGCGGGC CCTGAGAATC AAAGTTCTGT CACATTTTCC 
TCTCTCCCTT TATCTGTCCC ACCATCAGGG AACGTTTTGC CACAACCCGA AACCGATGTT 
ACGAACAAGA AGGTCATGGC TATGGAGCGC CAACTAGCGA GTTTAACCGG CATGGTTGAA 
TCCATTATAA CCAGTACCGA CGGTGTTCAA ACCAACAAGC CTGAACCCCC ATACCAGAAT 
CAACCAGTCG GTGGGAATCT CCCTAAAACG TCACACACCG TCCAACAAGT GATGACGTCA 
TCAGAGATGG GACAACAACT CTTGAAGTTG AGGGAAACAG CTCATAATCT GAGAGATCAA 
ATGACAGATC TGAAACGACA GCAGAGGATA AACATGGAAC ACGTGAACGA CATTGTGAGC 
GAGAAATCCC GGGAACTGGC TCAGTTTATC GCTGTCACAG CAGGCGCCCA GCACCGACCG 
TTGCGTATAC GAAGATTCCG ACTTGACCAG CAGAGACACG CTTACTCCGA GGATGCTACG 
ACCACGGAAC GAATGCTGTC GGAGGTGGAA GCCGCCGTAG AAGAACTAAG GAATGAAGTC 
GTGGAAAGAA GGTGCAGAGT TAACGCTCGT GAAGTCGAAG CTCTTGTTTT GCAACTCAGT 
CAAGCTGGAA GGACGGTAGC AAAGCTGAAA GGTTCATATC CTGCTCTATG TGAAGAGATA 
CGAACCGTAG GAGCAGGGGA AACGGAAGTC ATTTACGCTG AAGAGAAGTT CGTTGAAGCG 
GAACCGACAC GGTTGGATGA ACAGCTGAAA CGATGTCGGC AACTTACTGC AACACTTTTC 
ACTCTGAAAA AGTTGGCTAC AGTTCAAGAA GCGCGCAGTA AAACTCCGAC TTCATTCGGG 
GCAATTCGTC CGAACACACC GAGTCAACTC GGGGCTCGAA GAGGTGAAGA TCCGAATGCG 
AGCCGAGGAA GAATGCTCGA GGAAGTTCGG AGGACTCGGG TCGATCCTGT AAGAAGAATG 
GAGAGCATTC GGGATGCTGA GGTTAGTTGG ATACGAAGGC GAAAGTACAA CAGTGAATAC 
GACTCGAAAA AATTCGAGAA GGCACTGGAG TTGGCTGAGA AGGCACTTAA TCGAACCAAC 
CATCTTTCAC TGATCGACCA ACGTCGTCAT AACCACTTAA TGGACCTGTA CAAGAATCGT 
GACGTCATGA ACCACACACC ACGTAGCAAT CTCACAACTT CGGATTCGAG TTCCATTGAA 
TCGTTACCCC CTCCTCCGCC CCCTATGGAT CATATGGGTC ATCAATTCCA CCCTAATCAA 
CAACCGAGCT ATCCATTACA CCTCCCCCCT CCCCCAGCAG ACCTTAACCT TCCCGGCCCA 
CCACCACACG AGTATTCTGT CATCTCCCCC GACATCGGGT ATATGAGCTC GGATCTCTCC 
CCGGAGCATC GTGGCCAAAC ACAGACCGGC TCACTACCGA GGTATTCAAA CGCCACGCGG 
TTTCATGGGT ATCAGCAGCT TGAACGAAGC CATGGCACCA GCCACCCTGC TTATGTGGAT 
TACCACAATC ATGGCCGCCA GACGCCACAG ATGGTTGAAA GGTCACGATC GGTGACGCCA 
CACCACTGGA ACCAGCGTCC CATCACGCCA CAGATGTTTA CAAGCGAGAT ACCAGTCGGA 
CGCGAGTTGA CAGAAGATCA GCGACGTCAT ATATTGGAGC AACAACATCT AATGAACGAA 
GAGAAGAATT GCAAGTTAAA AGAACAATAT TCAAAGCTGC AACAACTACA ACGGAAGCAG 
AGAATGGCAA GCAAGAAATA TGTACCGAAG CCGAACTATC AGTCTATGTC GATGGCGGCT 
TCAGCAAAAT CGTCCGATGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026691610.1
CDS	2533..7236
Translation	MRTKSETLLLQPERVCLNGDDVKKVDCSTIYLEYSGECRTARLPNEVTSLDTIRALFVQAYPGLLTMPMLDPQRHSILLKNRKTGNYDEIDNVSRIKDRSFIRTRPIGGSGIPLPGTKKPTINHPKPAAKPVKPIPGNNRSKPRVTDDVKIAERNQKNLNKSGIKAPVKSLIRQYQSAPSLIKQAVTGSNEIKPVQSGEETQGKVKPPPPPRSAKPTVVAQTEAAQDQGNYSPNGTLLSGNGIGGGSIPQKTFSYVKSGNESPVNHQQQTITSPPQHRGLRQPTTSRLSPPSVEKKFSSIPTPGSKPKIQLPPNPTKPTDDEHHTKENVPRPKTPNILHQSPPTVTKLPPTSFAMTSPKNGAQRLPQPKPFGMKQHTTPANRFGAKRTSPPNVPNNNQLVPETAPVYSNIKRSASPERRATINGTGGSAGYCRGGRSFKGHQLKPVNIPSVPDTEIAAASNLEAKPDLNVPEVAQPVEPRQPISLPSDTKYSAMLPNPCTTDQVTSCSSSTSSDDVTDPGKSKMSTKKGLFGLRRLVSNRNKNPQNIPKRSQSEERPKTSDPSVNKSDLHSPSFNESLASFRSSSPALQVSTPVVRSSPIMSKSPAIPKSDLLNSLLTTNKETPEPNTDVMSTSSSDDDKDKPAPHPILAGVLKNNNLVKSTSSSSILSDSGASNVSMTSSIRTKKVSFQDDVTVCDGNGISTFSNGLREPDASVADVSFDRSMEQSLSVDTHEGAENEPKQWRPPSNGPPARNDFQQVGYRARPATPHRYEPPPYQPSPLAYQTNSPRSHQSAVAAAIQSISGNAETTSSAARYIPSQPEHFSVSCASPVPVVTRQGHEAPHKEEWNPLRRQSADASSNSPSITQRTCPTPVPTAPHKLAEVQVENEPSYFQTERAPSRSETFNSDTPPYYECCETTTPASGSITPSQLRDALKTQDEQYQHPVVESAGPENQSSVTFSSLPLSVPPSGNVLPQPETDVTNKKVMAMERQLASLTGMVESIITSTDGVQTNKPEPPYQNQPVGGNLPKTSHTVQQVMTSSEMGQQLLKLRETAHNLRDQMTDLKRQQRINMEHVNDIVSEKSRELAQFIAVTAGAQHRPLRIRRFRLDQQRHAYSEDATTTERMLSEVEAAVEELRNEVVERRCRVNAREVEALVLQLSQAGRTVAKLKGSYPALCEEIRTVGAGETEVIYAEEKFVEAEPTRLDEQLKRCRQLTATLFTLKKLATVQEARSKTPTSFGAIRPNTPSQLGARRGEDPNASRGRMLEEVRRTRVDPVRRMESIRDAEVSWIRRRKYNSEYDSKKFEKALELAEKALNRTNHLSLIDQRRHNHLMDLYKNRDVMNHTPRSNLTTSDSSSIESLPPPPPPMDHMGHQFHPNQQPSYPLHLPPPPADLNLPGPPPHEYSVISPDIGYMSSDLSPEHRGQTQTGSLPRYSNATRFHGYQQLERSHGTSHPAYVDYHNHGRQTPQMVERSRSVTPHHWNQRPITPQMFTSEIPVGRELTEDQRRHILEQQHLMNEEKNCKLKEQYSKLQQLQRKQRMASKKYVPKPNYQSMSMAASAKSSDV

Target ORF information:

RefSeq Version	XM_026835809.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis coiled-coil domain-containing protein CG32809 (LOC100185798), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026835809.1

ORF Insert Sequence:

ATGCGAACAA AGAGCGAAAC TCTCCTCCTG CAGCCAGAGA GGGTCTGTTT AAATGGTGAT 
GATGTTAAAA AAGTTGATTG TAGCACCATT TACTTGGAGT ATAGCGGAGA GTGTAGAACT 
GCCAGACTGC CGAACGAAGT GACAAGCCTT GATACAATAC GAGCTCTCTT CGTACAAGCA 
TACCCAGGAT TGCTAACGAT GCCAATGCTT GACCCACAAC GTCACTCCAT TTTATTAAAA 
AACAGGAAGA CGGGAAACTA CGATGAGATC GATAACGTGA GTCGCATCAA GGATCGTTCA 
TTCATTCGAA CTCGTCCGAT CGGGGGTTCA GGTATTCCAT TGCCTGGGAC CAAGAAGCCT 
ACAATCAACC ACCCGAAGCC TGCTGCTAAA CCAGTTAAGC CAATACCGGG CAACAACCGA 
TCAAAACCAC GGGTTACTGA TGATGTAAAA ATTGCAGAAC GCAACCAAAA GAACTTGAAC 
AAAAGTGGGA TAAAAGCACC GGTTAAATCC CTGATCCGTC AATACCAGTC TGCACCTAGT 
TTAATAAAGC AAGCAGTGAC CGGTTCAAAC GAGATAAAAC CTGTTCAAAG TGGCGAGGAG 
ACTCAGGGTA AAGTGAAACC GCCTCCCCCA CCGCGGTCCG CAAAGCCAAC AGTCGTAGCA 
CAAACAGAAG CAGCCCAAGA TCAAGGCAAT TATTCGCCTA ACGGGACTCT ATTATCAGGC 
AATGGGATTG GGGGTGGTAG TATACCACAG AAAACTTTCT CGTACGTTAA AAGCGGCAAT 
GAGTCTCCAG TAAACCACCA ACAACAGACA ATCACATCCC CACCGCAGCA TAGGGGCCTG 
AGGCAACCTA CAACTTCCAG GCTTTCCCCT CCATCTGTTG AGAAGAAGTT TTCCTCCATA 
CCAACGCCAG GGTCAAAGCC GAAGATCCAA CTCCCTCCGA ATCCAACAAA ACCAACCGAT 
GACGAACACC ACACTAAGGA GAACGTGCCA CGCCCAAAGA CTCCCAACAT ACTCCACCAA 
TCACCGCCCA CCGTCACCAA ACTTCCGCCT ACGTCATTCG CAATGACGTC ACCGAAAAAT 
GGCGCTCAAC GCCTTCCCCA ACCAAAACCT TTCGGCATGA AACAACATAC AACGCCAGCG 
AACCGGTTCG GTGCAAAACG AACGTCCCCT CCAAACGTCC CAAACAATAA TCAATTGGTG 
CCGGAAACTG CGCCGGTCTA TTCAAATATA AAGCGGTCAG CCTCACCGGA AAGAAGAGCT 
ACAATAAACG GTACCGGTGG ATCAGCAGGT TACTGCAGGG GTGGTAGGTC CTTTAAAGGC 
CACCAGCTGA AACCTGTAAA TATTCCATCC GTTCCCGACA CTGAAATAGC TGCAGCTTCT 
AACCTGGAAG CCAAACCTGA TCTCAACGTG CCGGAGGTAG CTCAACCTGT AGAACCGCGG 
CAACCCATTT CATTACCCAG TGATACCAAA TACTCTGCCA TGCTACCGAA CCCTTGTACC 
ACAGATCAAG TGACGTCATG CAGCTCCTCT ACGTCATCCG ATGACGTCAC CGACCCTGGA 
AAATCAAAGA TGTCAACGAA GAAGGGATTG TTCGGGCTTC GGAGATTGGT TTCGAACCGA 
AACAAGAACC CACAAAATAT TCCGAAACGA AGTCAAAGCG AGGAGCGACC GAAGACATCG 
GATCCTTCGG TAAATAAATC CGACCTTCAT TCTCCATCTT TCAACGAGTC CTTGGCTTCG 
TTTAGGTCAT CGTCCCCGGC TCTTCAAGTC AGCACGCCAG TGGTTCGATC TTCACCCATT 
ATGAGCAAGT CTCCTGCCAT ACCGAAAAGT GATCTTCTAA ATAGTTTGCT TACAACCAAC 
AAGGAGACAC CTGAGCCGAA CACAGATGTT ATGTCCACTT CCAGCTCGGA TGACGACAAG 
GACAAACCTG CTCCTCACCC CATACTGGCT GGAGTGCTTA AAAACAACAA CTTGGTCAAA 
AGCACTTCTT CCAGTTCGAT TTTGTCCGAC TCCGGCGCAA GTAACGTGTC TATGACGTCA 
TCCATACGTA CAAAGAAGGT TTCATTTCAA GACGACGTAA CAGTTTGTGA TGGTAATGGG 
ATCAGTACGT TTAGTAACGG ATTGCGAGAA CCTGACGCTT CTGTAGCTGA TGTATCGTTC 
GATAGGTCGA TGGAACAATC GTTATCTGTC GACACGCATG AGGGTGCGGA GAATGAGCCA 
AAGCAGTGGC GACCGCCCTC TAACGGTCCG CCTGCTCGAA ATGATTTCCA ACAGGTGGGC 
TACAGAGCAA GACCCGCAAC ACCACACAGG TATGAACCAC CACCATACCA ACCCTCCCCT 
CTTGCATACC AAACCAACTC TCCTCGATCG CACCAATCAG CTGTGGCCGC TGCTATACAA 
AGCATAAGTG GGAATGCGGA AACAACAAGC AGTGCTGCAA GGTACATTCC AAGCCAACCT 
GAACATTTCT CGGTATCTTG CGCATCACCT GTGCCAGTAG TAACCAGGCA GGGGCACGAG 
GCTCCACACA AGGAGGAATG GAATCCTCTC CGACGACAAT CCGCAGACGC AAGCTCTAAC 
TCACCTTCAA TTACACAGCG GACATGTCCT ACCCCTGTGC CAACCGCCCC GCATAAGTTA 
GCTGAGGTAC AAGTAGAAAA CGAGCCTTCA TATTTCCAAA CAGAGCGAGC GCCATCTCGT 
TCTGAAACAT TTAACTCCGA CACACCCCCC TACTACGAAT GCTGCGAAAC TACGACGCCC 
GCCTCTGGCT CAATTACACC AAGTCAATTA AGAGATGCGT TAAAGACACA GGACGAACAG 
TATCAACATC CAGTCGTTGA ATCTGCGGGC CCTGAGAATC AAAGTTCTGT CACATTTTCC 
TCTCTCCCTT TATCTGTCCC ACCATCAGGG AACGTTTTGC CACAACCCGA AACCGATGTT 
ACGAACAAGA AGGTCATGGC TATGGAGCGC CAACTAGCGA GTTTAACCGG CATGGTTGAA 
TCCATTATAA CCAGTACCGA CGGTGTTCAA ACCAACAAGC CTGAACCCCC ATACCAGAAT 
CAACCAGTCG GTGGGAATCT CCCTAAAACG TCACACACCG TCCAACAAGT GATGACGTCA 
TCAGAGATGG GACAACAACT CTTGAAGTTG AGGGAAACAG CTCATAATCT GAGAGATCAA 
ATGACAGATC TGAAACGACA GCAGAGGATA AACATGGAAC ACGTGAACGA CATTGTGAGC 
GAGAAATCCC GGGAACTGGC TCAGTTTATC GCTGTCACAG CAGGCGCCCA GCACCGACCG 
TTGCGTATAC GAAGATTCCG ACTTGACCAG CAGAGACACG CTTACTCCGA GGATGCTACG 
ACCACGGAAC GAATGCTGTC GGAGGTGGAA GCCGCCGTAG AAGAACTAAG GAATGAAGTC 
GTGGAAAGAA GGTGCAGAGT TAACGCTCGT GAAGTCGAAG CTCTTGTTTT GCAACTCAGT 
CAAGCTGGAA GGACGGTAGC AAAGCTGAAA GGTTCATATC CTGCTCTATG TGAAGAGATA 
CGAACCGTAG GAGCAGGGGA AACGGAAGTC ATTTACGCTG AAGAGAAGTT CGTTGAAGCG 
GAACCGACAC GGTTGGATGA ACAGCTGAAA CGATGTCGGC AACTTACTGC AACACTTTTC 
ACTCTGAAAA AGTTGGCTAC AGTTCAAGAA GCGCGCAGTA AAACTCCGAC TTCATTCGGG 
GCAATTCGTC CGAACACACC GAGTCAACTC GGGGCTCGAA GAGGTGAAGA TCCGAATGCG 
AGCCGAGGAA GAATGCTCGA GGAAGTTCGG AGGACTCGGG TCGATCCTGT AAGAAGAATG 
GAGAGCATTC GGGATGCTGA GGTTAGTTGG ATACGAAGGC GAAAGTACAA CAGTGAATAC 
GACTCGAAAA AATTCGAGAA GGCACTGGAG TTGGCTGAGA AGGCACTTAA TCGAACCAAC 
CATCTTTCAC TGATCGACCA ACGTCGTCAT AACCACTTAA TGGACCTGTA CAAGAATCGT 
GACGTCATGA ACCACACACC ACGTAGCAAT CTCACAACTT CGGATTCGAG TTCCATTGAA 
TCGTTACCCC CTCCTCCGCC CCCTATGGAT CATATGGGTC ATCAATTCCA CCCTAATCAA 
CAACCGAGCT ATCCATTACA CCTCCCCCCT CCCCCAGCAG ACCTTAACCT TCCCGGCCCA 
CCACCACACG AGTATTCTGT CATCTCCCCC GACATCGGGT ATATGAGCTC GGATCTCTCC 
CCGGAGCATC GTGGCCAAAC ACAGACCGGC TCACTACCGA GGTATTCAAA CGCCACGCGG 
TTTCATGGGT ATCAGCAGCT TGAACGAAGC CATGGCACCA GCCACCCTGC TTATGTGGAT 
TACCACAATC ATGGCCGCCA GACGCCACAG ATGGTTGAAA GGTCACGATC GGTGACGCCA 
CACCACTGGA ACCAGCGTCC CATCACGCCA CAGATGTTTA CAAGCGAGAT ACCAGTCGGA 
CGCGAGTTGA CAGAAGATCA GCGACGTCAT ATATTGGAGC AACAACATCT AATGAACGAA 
GAGAAGAATT GCAAGTTAAA AGAACAATAT TCAAAGCTGC AACAACTACA ACGGAAGCAG 
AGAATGGCAA GCAAGAAATA TGTACCGAAG CCGAACTATC AGTCTATGTC GATGGCGGCT 
TCAGCAAAAT CGTCCGATGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100185798 ( XM_026835810.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_026691611.1 Ciona intestinalis coiled-coil domain-containing protein CG32809 (LOC100185798), transcript variant X5, mRNA.

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CloneID	OCi156737
Clone ID Related Accession (Same CDS sequence)	XM_026835810.1
Accession Version	XM_026835810.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1540051200000
Organism	Ciona intestinalis(vase tunicate)
Product	uncharacterized protein LOC100185798 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020174.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ciona intestinalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAATGC TTGACCCACA ACGTCACTCC ATTTTATTAA AAAACAGGAA GACGGGAAAC 
TACGATGAGA TCGATAACGT GAGTCGCATC AAGGATCGTT CATTCATTCG AACTCGTCCG 
ATCGGGGGTT CAGGTATTCC ATTGCCTGGG ACCAAGAAGC CTACAATCAA CCACCCGAAG 
CCTGCTGCTA AACCAGTTAA GCCAATACCG GGCAACAACC GATCAAAACC ACGGGTTACT 
GATGATGTAA AAATTGCAGA ACGCAACCAA AAGAACTTGA ACAAAAGTGG GATAAAAGCA 
CCGGTTAAAT CCCTGATCCG TCAATACCAG TCTGCACCTA GTTTAATAAA GCAAGCAGTG 
ACCGGTTCAA ACGAGATAAA ACCTGTTCAA AGTGGCGAGG AGACTCAGGG TAAAGTGAAA 
CCGCCTCCCC CACCGCGGTC CGCAAAGCCA ACAGTCGTAG CACAAACAGA AGCAGCCCAA 
GATCAAGGCA ATTATTCGCC TAACGGGACT CTATTATCAG GCAATGGGAT TGGGGGTGGT 
AGTATACCAC AGAAAACTTT CTCGTACGTT AAAAGCGGCA ATGAGTCTCC AGTAAACCAC 
CAACAACAGA CAATCACATC CCCACCGCAG CATAGGGGCC TGAGGCAACC TACAACTTCC 
AGGCTTTCCC CTCCATCTGT TGAGAAGAAG TTTTCCTCCA TACCAACGCC AGGGTCAAAG 
CCGAAGATCC AACTCCCTCC GAATCCAACA AAACCAACCG ATGACGAACA CCACACTAAG 
GAGAACGTGC CACGCCCAAA GACTCCCAAC ATACTCCACC AATCACCGCC CACCGTCACC 
AAACTTCCGC CTACGTCATT CGCAATGACG TCACCGAAAA ATGGCGCTCA ACGCCTTCCC 
CAACCAAAAC CTTTCGGCAT GAAACAACAT ACAACGCCAG CGAACCGGTT CGGTGCAAAA 
CGAACGTCCC CTCCAAACGT CCCAAACAAT AATCAATTGG TGCCGGAAAC TGCGCCGGTC 
TATTCAAATA TAAAGCGGTC AGCCTCACCG GAAAGAAGAG CTACAATAAA CGGTACCGGT 
GGATCAGCAG GTTACTGCAG GGGTGGTAGG TCCTTTAAAG GCCACCAGCT GAAACCTGTA 
AATATTCCAT CCGTTCCCGA CACTGAAATA GCTGCAGCTT CTAACCTGGA AGCCAAACCT 
GATCTCAACG TGCCGGAGGT AGCTCAACCT GTAGAACCGC GGCAACCCAT TTCATTACCC 
AGTGATACCA AATACTCTGC CATGCTACCG AACCCTTGTA CCACAGATCA AGTGACGTCA 
TGCAGCTCCT CTACGTCATC CGATGACGTC ACCGACCCTG GAAAATCAAA GATGTCAACG 
AAGAAGGGAT TGTTCGGGCT TCGGAGATTG GTTTCGAACC GAAACAAGAA CCCACAAAAT 
ATTCCGAAAC GAAGTCAAAG CGAGGAGCGA CCGAAGACAT CGGATCCTTC GGTAAATAAA 
TCCGACCTTC ATTCTCCATC TTTCAACGAG TCCTTGGCTT CGTTTAGGTC ATCGTCCCCG 
GCTCTTCAAG TCAGCACGCC AGTGGTTCGA TCTTCACCCA TTATGAGCAA GTCTCCTGCC 
ATACCGAAAA GTGATCTTCT AAATAGTTTG CTTACAACCA ACAAGGAGAC ACCTGAGCCG 
AACACAGATG TTATGTCCAC TTCCAGCTCG GATGACGACA AGGACAAACC TGCTCCTCAC 
CCCATACTGG CTGGAGTGCT TAAAAACAAC AACTTGGTCA AAAGCACTTC TTCCAGTTCG 
ATTTTGTCCG ACTCCGGCGC AAGTAACGTG TCTATGACGT CATCCATACG TACAAAGAAG 
GTTTCATTTC AAGACGACGT AACAGTTTGT GATGGTAATG GGATCAGTAC GTTTAGTAAC 
GGATTGCGAG AACCTGACGC TTCTGTAGCT GATGTATCGT TCGATAGGTC GATGGAACAA 
TCGTTATCTG TCGACACGCA TGAGGGTGCG GAGAATGAGC CAAAGCAGTG GCGACCGCCC 
TCTAACGGTC CGCCTGCTCG AAATGATTTC CAACAGGTGG GCTACAGAGC AAGACCCGCA 
ACACCACACA GGTATGAACC ACCACCATAC CAACCCTCCC CTCTTGCATA CCAAACCAAC 
TCTCCTCGAT CGCACCAATC AGCTGTGGCC GCTGCTATAC AAAGCATAAG TGGGAATGCG 
GAAACAACAA GCAGTGCTGC AAGGTACATT CCAAGCCAAC CTGAACATTT CTCGGTATCT 
TGCGCATCAC CTGTGCCAGT AGTAACCAGG CAGGGGCACG AGGCTCCACA CAAGGAGGAA 
TGGAATCCTC TCCGACGACA ATCCGCAGAC GCAAGCTCTA ACTCACCTTC AATTACACAG 
CGGACATGTC CTACCCCTGT GCCAACCGCC CCGCATAAGT TAGCTGAGGT ACAAGTAGAA 
AACGAGCCTT CATATTTCCA AACAGAGCGA GCGCCATCTC GTTCTGAAAC ATTTAACTCC 
GACACACCCC CCTACTACGA ATGCTGCGAA ACTACGACGC CCGCCTCTGG CTCAATTACA 
CCAAGTCAAT TAAGAGATGC GTTAAAGACA CAGGACGAAC AGTATCAACA TCCAGTCGTT 
GAATCTGCGG GCCCTGAGAA TCAAAGTTCT GTCACATTTT CCTCTCTCCC TTTATCTGTC 
CCACCATCAG GGAACGTTTT GCCACAACCC GAAACCGATG TTACGAACAA GAAGGTCATG 
GCTATGGAGC GCCAACTAGC GAGTTTAACC GGCATGGTTG AATCCATTAT AACCAGTACC 
GACGGTGTTC AAACCAACAA GCCTGAACCC CCATACCAGA ATCAACCAGT CGGTGGGAAT 
CTCCCTAAAA CGTCACACAC CGTCCAACAA GTGATGACGT CATCAGAGAT GGGACAACAA 
CTCTTGAAGT TGAGGGAAAC AGCTCATAAT CTGAGAGATC AAATGACAGA TCTGAAACGA 
CAGCAGAGGA TAAACATGGA ACACGTGAAC GACATTGTGA GCGAGAAATC CCGGGAACTG 
GCTCAGTTTA TCGCTGTCAC AGCAGGCGCC CAGCACCGAC CGTTGCGTAT ACGAAGATTC 
CGACTTGACC AGCAGAGACA CGCTTACTCC GAGGATGCTA CGACCACGGA ACGAATGCTG 
TCGGAGGTGG AAGCCGCCGT AGAAGAACTA AGGAATGAAG TCGTGGAAAG AAGGTGCAGA 
GTTAACGCTC GTGAAGTCGA AGCTCTTGTT TTGCAACTCA GTCAAGCTGG AAGGACGGTA 
GCAAAGCTGA AAGGTTCATA TCCTGCTCTA TGTGAAGAGA TACGAACCGT AGGAGCAGGG 
GAAACGGAAG TCATTTACGC TGAAGAGAAG TTCGTTGAAG CGGAACCGAC ACGGTTGGAT 
GAACAGCTGA AACGATGTCG GCAACTTACT GCAACACTTT TCACTCTGAA AAAGTTGGCT 
ACAGTTCAAG AAGCGCGCAG TAAAACTCCG ACTTCATTCG GGGCAATTCG TCCGAACACA 
CCGAGTCAAC TCGGGGCTCG AAGAGGTGAA GATCCGAATG CGAGCCGAGG AAGAATGCTC 
GAGGAAGTTC GGAGGACTCG GGTCGATCCT GTAAGAAGAA TGGAGAGCAT TCGGGATGCT 
GAGGTTAGTT GGATACGAAG GCGAAAGTAC AACAGTGAAT ACGACTCGAA AAAATTCGAG 
AAGGCACTGG AGTTGGCTGA GAAGGCACTT AATCGAACCA ACCATCTTTC ACTGATCGAC 
CAACGTCGTC ATAACCACTT AATGGACCTG TACAAGAATC GTGACGTCAT GAACCACACA 
CCACGTAGCA ATCTCACAAC TTCGGATTCG AGTTCCATTG AATCGTTACC CCCTCCTCCG 
CCCCCTATGG ATCATATGGG TCATCAATTC CACCCTAATC AACAACCGAG CTATCCATTA 
CACCTCCCCC CTCCCCCAGC AGACCTTAAC CTTCCCGGCC CACCACCACA CGAGTATTCT 
GTCATCTCCC CCGACATCGG GTATATGAGC TCGGATCTCT CCCCGGAGCA TCGTGGCCAA 
ACACAGACCG GCTCACTACC GAGGTATTCA AACGCCACGC GGTTTCATGG GTATCAGCAG 
CTTGAACGAA GCCATGGCAC CAGCCACCCT GCTTATGTGG ATTACCACAA TCATGGCCGC 
CAGACGCCAC AGATGGTTGA AAGGTCACGA TCGGTGACGC CACACCACTG GAACCAGCGT 
CCCATCACGC CACAGATGTT TACAAGCGAG ATACCAGTCG GACGCGAGTT GACAGAAGAT 
CAGCGACGTC ATATATTGGA GCAACAACAT CTAATGAACG AAGAGAAGAA TTGCAAGTTA 
AAAGAACAAT ATTCAAAGCT GCAACAACTA CAACGGAAGC AGAGAATGGC AAGCAAGAAA 
TATGTACCGA AGCCGAACTA TCAGTCTATG TCGATGGCGG CTTCAGCAAA ATCGTCCGAT 
GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026691611.1
CDS	409..4914
Translation	MPMLDPQRHSILLKNRKTGNYDEIDNVSRIKDRSFIRTRPIGGSGIPLPGTKKPTINHPKPAAKPVKPIPGNNRSKPRVTDDVKIAERNQKNLNKSGIKAPVKSLIRQYQSAPSLIKQAVTGSNEIKPVQSGEETQGKVKPPPPPRSAKPTVVAQTEAAQDQGNYSPNGTLLSGNGIGGGSIPQKTFSYVKSGNESPVNHQQQTITSPPQHRGLRQPTTSRLSPPSVEKKFSSIPTPGSKPKIQLPPNPTKPTDDEHHTKENVPRPKTPNILHQSPPTVTKLPPTSFAMTSPKNGAQRLPQPKPFGMKQHTTPANRFGAKRTSPPNVPNNNQLVPETAPVYSNIKRSASPERRATINGTGGSAGYCRGGRSFKGHQLKPVNIPSVPDTEIAAASNLEAKPDLNVPEVAQPVEPRQPISLPSDTKYSAMLPNPCTTDQVTSCSSSTSSDDVTDPGKSKMSTKKGLFGLRRLVSNRNKNPQNIPKRSQSEERPKTSDPSVNKSDLHSPSFNESLASFRSSSPALQVSTPVVRSSPIMSKSPAIPKSDLLNSLLTTNKETPEPNTDVMSTSSSDDDKDKPAPHPILAGVLKNNNLVKSTSSSSILSDSGASNVSMTSSIRTKKVSFQDDVTVCDGNGISTFSNGLREPDASVADVSFDRSMEQSLSVDTHEGAENEPKQWRPPSNGPPARNDFQQVGYRARPATPHRYEPPPYQPSPLAYQTNSPRSHQSAVAAAIQSISGNAETTSSAARYIPSQPEHFSVSCASPVPVVTRQGHEAPHKEEWNPLRRQSADASSNSPSITQRTCPTPVPTAPHKLAEVQVENEPSYFQTERAPSRSETFNSDTPPYYECCETTTPASGSITPSQLRDALKTQDEQYQHPVVESAGPENQSSVTFSSLPLSVPPSGNVLPQPETDVTNKKVMAMERQLASLTGMVESIITSTDGVQTNKPEPPYQNQPVGGNLPKTSHTVQQVMTSSEMGQQLLKLRETAHNLRDQMTDLKRQQRINMEHVNDIVSEKSRELAQFIAVTAGAQHRPLRIRRFRLDQQRHAYSEDATTTERMLSEVEAAVEELRNEVVERRCRVNAREVEALVLQLSQAGRTVAKLKGSYPALCEEIRTVGAGETEVIYAEEKFVEAEPTRLDEQLKRCRQLTATLFTLKKLATVQEARSKTPTSFGAIRPNTPSQLGARRGEDPNASRGRMLEEVRRTRVDPVRRMESIRDAEVSWIRRRKYNSEYDSKKFEKALELAEKALNRTNHLSLIDQRRHNHLMDLYKNRDVMNHTPRSNLTTSDSSSIESLPPPPPPMDHMGHQFHPNQQPSYPLHLPPPPADLNLPGPPPHEYSVISPDIGYMSSDLSPEHRGQTQTGSLPRYSNATRFHGYQQLERSHGTSHPAYVDYHNHGRQTPQMVERSRSVTPHHWNQRPITPQMFTSEIPVGRELTEDQRRHILEQQHLMNEEKNCKLKEQYSKLQQLQRKQRMASKKYVPKPNYQSMSMAASAKSSDV

Target ORF information:

RefSeq Version	XM_026835810.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis coiled-coil domain-containing protein CG32809 (LOC100185798), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026835810.1

ORF Insert Sequence:

ATGCCAATGC TTGACCCACA ACGTCACTCC ATTTTATTAA AAAACAGGAA GACGGGAAAC 
TACGATGAGA TCGATAACGT GAGTCGCATC AAGGATCGTT CATTCATTCG AACTCGTCCG 
ATCGGGGGTT CAGGTATTCC ATTGCCTGGG ACCAAGAAGC CTACAATCAA CCACCCGAAG 
CCTGCTGCTA AACCAGTTAA GCCAATACCG GGCAACAACC GATCAAAACC ACGGGTTACT 
GATGATGTAA AAATTGCAGA ACGCAACCAA AAGAACTTGA ACAAAAGTGG GATAAAAGCA 
CCGGTTAAAT CCCTGATCCG TCAATACCAG TCTGCACCTA GTTTAATAAA GCAAGCAGTG 
ACCGGTTCAA ACGAGATAAA ACCTGTTCAA AGTGGCGAGG AGACTCAGGG TAAAGTGAAA 
CCGCCTCCCC CACCGCGGTC CGCAAAGCCA ACAGTCGTAG CACAAACAGA AGCAGCCCAA 
GATCAAGGCA ATTATTCGCC TAACGGGACT CTATTATCAG GCAATGGGAT TGGGGGTGGT 
AGTATACCAC AGAAAACTTT CTCGTACGTT AAAAGCGGCA ATGAGTCTCC AGTAAACCAC 
CAACAACAGA CAATCACATC CCCACCGCAG CATAGGGGCC TGAGGCAACC TACAACTTCC 
AGGCTTTCCC CTCCATCTGT TGAGAAGAAG TTTTCCTCCA TACCAACGCC AGGGTCAAAG 
CCGAAGATCC AACTCCCTCC GAATCCAACA AAACCAACCG ATGACGAACA CCACACTAAG 
GAGAACGTGC CACGCCCAAA GACTCCCAAC ATACTCCACC AATCACCGCC CACCGTCACC 
AAACTTCCGC CTACGTCATT CGCAATGACG TCACCGAAAA ATGGCGCTCA ACGCCTTCCC 
CAACCAAAAC CTTTCGGCAT GAAACAACAT ACAACGCCAG CGAACCGGTT CGGTGCAAAA 
CGAACGTCCC CTCCAAACGT CCCAAACAAT AATCAATTGG TGCCGGAAAC TGCGCCGGTC 
TATTCAAATA TAAAGCGGTC AGCCTCACCG GAAAGAAGAG CTACAATAAA CGGTACCGGT 
GGATCAGCAG GTTACTGCAG GGGTGGTAGG TCCTTTAAAG GCCACCAGCT GAAACCTGTA 
AATATTCCAT CCGTTCCCGA CACTGAAATA GCTGCAGCTT CTAACCTGGA AGCCAAACCT 
GATCTCAACG TGCCGGAGGT AGCTCAACCT GTAGAACCGC GGCAACCCAT TTCATTACCC 
AGTGATACCA AATACTCTGC CATGCTACCG AACCCTTGTA CCACAGATCA AGTGACGTCA 
TGCAGCTCCT CTACGTCATC CGATGACGTC ACCGACCCTG GAAAATCAAA GATGTCAACG 
AAGAAGGGAT TGTTCGGGCT TCGGAGATTG GTTTCGAACC GAAACAAGAA CCCACAAAAT 
ATTCCGAAAC GAAGTCAAAG CGAGGAGCGA CCGAAGACAT CGGATCCTTC GGTAAATAAA 
TCCGACCTTC ATTCTCCATC TTTCAACGAG TCCTTGGCTT CGTTTAGGTC ATCGTCCCCG 
GCTCTTCAAG TCAGCACGCC AGTGGTTCGA TCTTCACCCA TTATGAGCAA GTCTCCTGCC 
ATACCGAAAA GTGATCTTCT AAATAGTTTG CTTACAACCA ACAAGGAGAC ACCTGAGCCG 
AACACAGATG TTATGTCCAC TTCCAGCTCG GATGACGACA AGGACAAACC TGCTCCTCAC 
CCCATACTGG CTGGAGTGCT TAAAAACAAC AACTTGGTCA AAAGCACTTC TTCCAGTTCG 
ATTTTGTCCG ACTCCGGCGC AAGTAACGTG TCTATGACGT CATCCATACG TACAAAGAAG 
GTTTCATTTC AAGACGACGT AACAGTTTGT GATGGTAATG GGATCAGTAC GTTTAGTAAC 
GGATTGCGAG AACCTGACGC TTCTGTAGCT GATGTATCGT TCGATAGGTC GATGGAACAA 
TCGTTATCTG TCGACACGCA TGAGGGTGCG GAGAATGAGC CAAAGCAGTG GCGACCGCCC 
TCTAACGGTC CGCCTGCTCG AAATGATTTC CAACAGGTGG GCTACAGAGC AAGACCCGCA 
ACACCACACA GGTATGAACC ACCACCATAC CAACCCTCCC CTCTTGCATA CCAAACCAAC 
TCTCCTCGAT CGCACCAATC AGCTGTGGCC GCTGCTATAC AAAGCATAAG TGGGAATGCG 
GAAACAACAA GCAGTGCTGC AAGGTACATT CCAAGCCAAC CTGAACATTT CTCGGTATCT 
TGCGCATCAC CTGTGCCAGT AGTAACCAGG CAGGGGCACG AGGCTCCACA CAAGGAGGAA 
TGGAATCCTC TCCGACGACA ATCCGCAGAC GCAAGCTCTA ACTCACCTTC AATTACACAG 
CGGACATGTC CTACCCCTGT GCCAACCGCC CCGCATAAGT TAGCTGAGGT ACAAGTAGAA 
AACGAGCCTT CATATTTCCA AACAGAGCGA GCGCCATCTC GTTCTGAAAC ATTTAACTCC 
GACACACCCC CCTACTACGA ATGCTGCGAA ACTACGACGC CCGCCTCTGG CTCAATTACA 
CCAAGTCAAT TAAGAGATGC GTTAAAGACA CAGGACGAAC AGTATCAACA TCCAGTCGTT 
GAATCTGCGG GCCCTGAGAA TCAAAGTTCT GTCACATTTT CCTCTCTCCC TTTATCTGTC 
CCACCATCAG GGAACGTTTT GCCACAACCC GAAACCGATG TTACGAACAA GAAGGTCATG 
GCTATGGAGC GCCAACTAGC GAGTTTAACC GGCATGGTTG AATCCATTAT AACCAGTACC 
GACGGTGTTC AAACCAACAA GCCTGAACCC CCATACCAGA ATCAACCAGT CGGTGGGAAT 
CTCCCTAAAA CGTCACACAC CGTCCAACAA GTGATGACGT CATCAGAGAT GGGACAACAA 
CTCTTGAAGT TGAGGGAAAC AGCTCATAAT CTGAGAGATC AAATGACAGA TCTGAAACGA 
CAGCAGAGGA TAAACATGGA ACACGTGAAC GACATTGTGA GCGAGAAATC CCGGGAACTG 
GCTCAGTTTA TCGCTGTCAC AGCAGGCGCC CAGCACCGAC CGTTGCGTAT ACGAAGATTC 
CGACTTGACC AGCAGAGACA CGCTTACTCC GAGGATGCTA CGACCACGGA ACGAATGCTG 
TCGGAGGTGG AAGCCGCCGT AGAAGAACTA AGGAATGAAG TCGTGGAAAG AAGGTGCAGA 
GTTAACGCTC GTGAAGTCGA AGCTCTTGTT TTGCAACTCA GTCAAGCTGG AAGGACGGTA 
GCAAAGCTGA AAGGTTCATA TCCTGCTCTA TGTGAAGAGA TACGAACCGT AGGAGCAGGG 
GAAACGGAAG TCATTTACGC TGAAGAGAAG TTCGTTGAAG CGGAACCGAC ACGGTTGGAT 
GAACAGCTGA AACGATGTCG GCAACTTACT GCAACACTTT TCACTCTGAA AAAGTTGGCT 
ACAGTTCAAG AAGCGCGCAG TAAAACTCCG ACTTCATTCG GGGCAATTCG TCCGAACACA 
CCGAGTCAAC TCGGGGCTCG AAGAGGTGAA GATCCGAATG CGAGCCGAGG AAGAATGCTC 
GAGGAAGTTC GGAGGACTCG GGTCGATCCT GTAAGAAGAA TGGAGAGCAT TCGGGATGCT 
GAGGTTAGTT GGATACGAAG GCGAAAGTAC AACAGTGAAT ACGACTCGAA AAAATTCGAG 
AAGGCACTGG AGTTGGCTGA GAAGGCACTT AATCGAACCA ACCATCTTTC ACTGATCGAC 
CAACGTCGTC ATAACCACTT AATGGACCTG TACAAGAATC GTGACGTCAT GAACCACACA 
CCACGTAGCA ATCTCACAAC TTCGGATTCG AGTTCCATTG AATCGTTACC CCCTCCTCCG 
CCCCCTATGG ATCATATGGG TCATCAATTC CACCCTAATC AACAACCGAG CTATCCATTA 
CACCTCCCCC CTCCCCCAGC AGACCTTAAC CTTCCCGGCC CACCACCACA CGAGTATTCT 
GTCATCTCCC CCGACATCGG GTATATGAGC TCGGATCTCT CCCCGGAGCA TCGTGGCCAA 
ACACAGACCG GCTCACTACC GAGGTATTCA AACGCCACGC GGTTTCATGG GTATCAGCAG 
CTTGAACGAA GCCATGGCAC CAGCCACCCT GCTTATGTGG ATTACCACAA TCATGGCCGC 
CAGACGCCAC AGATGGTTGA AAGGTCACGA TCGGTGACGC CACACCACTG GAACCAGCGT 
CCCATCACGC CACAGATGTT TACAAGCGAG ATACCAGTCG GACGCGAGTT GACAGAAGAT 
CAGCGACGTC ATATATTGGA GCAACAACAT CTAATGAACG AAGAGAAGAA TTGCAAGTTA 
AAAGAACAAT ATTCAAAGCT GCAACAACTA CAACGGAAGC AGAGAATGGC AAGCAAGAAA 
TATGTACCGA AGCCGAACTA TCAGTCTATG TCGATGGCGG CTTCAGCAAA ATCGTCCGAT 
GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100185798 ( XM_018813181.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_018668726.1 Ciona intestinalis uncharacterized LOC100185798 (LOC100185798), mRNA.

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CloneID	OCi74452
Clone ID Related Accession (Same CDS sequence)	XM_018813181.1 , XM_018813181.2
Accession Version	XM_018813181.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1476892800000
Organism	Ciona intestinalis(vase tunicate)
Product	uncharacterized protein LOC100185798
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020174.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ciona intestinalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGATGAAA ATACACAAGA AGCTGGCGAC TCAGAAGTCG AAAATGACAA GGCAAGTTAC 
GCGATGTTGA GACCCTTGCA GAAAGTGATC GCTCACTTTG AGATGAAACT TGCCGCCAAC 
AAAGGGAACA TTCCAACACC CAGACGAGAA ATACCAAACC TGAAACCGCA AGCTGAGCGT 
CTTCGTCAGA GCAGGAAAAC GTCCAAACAA CGAAGTTTTA TTAAAAGCAG TGGGAAGCTA 
TTGTCAAGCG TGCAAGAGAA GGTTACTGTT GGATCTAAAT CGTCAACACC TTGTCATAGC 
AGATCAAGTT CGGTGTGTTC AAGTAATGTG GAAAGTCATG TACCTACGTC AGAAGGCCCT 
CCCATGACAT CATCGCAACA AGATTTTGAC ACCACTTCTG TTTCGTCTAC GTCCAGTATG 
ACGTCAGAAA CTGTAGATTC TCAAGTTAAA TATTCCTCGT TACAAAGAAA CTACCAACAT 
GGGCACAAGA GTTTTCAGTT CAACACTCTG GATGGAAGGA GAACTCGATC TAAGAGTGGA 
GGCCACCATA ATCGAAGTTT CTATCCGCAA GATGGCAGTA TATCCCCAGC CGAAGCAGAT 
CGAAAGCGCT CAGCCTTTTT TCAATATTTA GTCCAAAAAT ACCCGCAACA CTCCGATGTT 
ATTGTTGGGA AATCATTTCA GCAAACCCAG ACTCCAGCAC CTTCCTTCGG TGGGATTCGA 
ACCCGTGCAA GCTACGCTGC CGATTGCTCT TATAACTCTT CATCTCCTAA CGACAGCGAG 
GTCACGTGGC ATAACAGTTT GTCACGTGGT AGTAGACTCA GGTCCAGCCT CCCAATCGTA 
AGAAGTTTAA GTCGAAGCCG AGAACGACCA TTAGGCACCA TTTACTTGGA GTATAGCGGA 
GAGTGTAGAA CTGCCAGACT GCCGAACGAA GTGACAAGCC TTGATACAAT ACGAGCTCTC 
TTCGTACAAG CATACCCAGG ATTGCTAACG ATGCCAATGC TTGACCCACA ACGTCACTCC 
ATTTTATTAA AAAACAGGAA GACGGGAAAC TACGATGAGA TCGATAACGT GAGTCGCATC 
AAGGATCGTT CATTCATTCG AACTCGTCCG ATCGGGGGTT CAGGTATTCC ATTGCCTGGG 
ACCAAGAAGC CTACAATCAA CCACCCGAAG CCTGCTGCTA AACCAGTTAA GCCAATACCG 
GGCAACAACC GATCAAAACC ACGGGTTACT GATGATGTAA AAATTGCAGA ACGCAACCAA 
AAGAACTTGA ACAAAAGTGG GATAAAAGCA CCGGTTAAAT CCCTGATCCG TCAATACCAG 
TCTGCACCTA GTTTAATAAA GCAAGCAGTG ACCGGTTCAA ACGAGATAAA ACCTGTTCAA 
AGTGGCGAGG AGACTCAGGG TAAAGTGAAA CCGCCTCCCC CACCGCGGTC CGCAAAGCCA 
ACAGTCGTAG CACAAACAGA AGCAGCCCAA GATCAAGGCA ATTATTCGCC TAACGGGACT 
CTATTATCAG GCAATGGGAT TGGGGGTGGT AGTATACCAC AGAAAACTTT CTCGTACGTT 
AAAAGCGGCA ATGAGTCTCC AGTAAACCAC CAACAACAGA CAATCACATC CCCACCGCAG 
CATAGGGGCC TGAGGCAACC TACAACTTCC AGGCTTTCCC CTCCATCTGT TGAGAAGAAG 
TTTTCCTCCA TACCAACGCC AGGGTCAAAG CCGAAGATCC AACTCCCTCC GAATCCAACA 
AAACCAACCG ATGACGAACA CCACACTAAG GAGAACGTGC CACGCCCAAA GACTCCCAAC 
ATACTCCACC AATCACCGCC CACCGTCACC AAACTTCCGC CTACGTCATT CGCAATGACG 
TCACCGAAAA ATGGCGCTCA ACGCCTTCCC CAACCAAAAC CTTTCGGCAT GAAACAACAT 
ACAACGCCAG CGAACCGGTT CGGTGCAAAA CGAACGTCCC CTCCAAACGT CCCAAACAAT 
AATCAATTGG TGCCGGAAAC TGCGCCGGTC TATTCAAATA TAAAGCGGTC AGCCTCACCG 
GAAAGAAGAG CTACAATAAA CGGTACCGGT GGATCAGCAG GTTACTGCAG GGGTGGTAGG 
TCCTTTAAAG GCCACCAGCT GAAACCTGTA AATATTCCAT CCGTTCCCGA CACTGAAATA 
GCTGCAGCTT CTAACCTGGA AGCCAAACCT GATCTCAACG TGCCGGAGGT AGCTCAACCT 
GTAGAACCGC GGCAACCCAT TTCATTACCC AGTGATACCA AATACTCTGC CATGCTACCG 
AACCCTTGTA CCACAGATCA AGTGACGTCA TGCAGCTCCT CTACGTCATC CGATGACGTC 
ACCGACCCTG GAAAATCAAA GATGTCAACG AAGAAGGGAT TGTTCGGGCT TCGGAGATTG 
GTTTCGAACC GAAACAAGAA CCCACAAAAT ATTCCGAAAC GAAGTCAAAG CGAGGAGCGA 
CCGAAGACAT CGGATCCTTC GGTAAATAAA TCCGACCTTC ATTCTCCATC TTTCAACGAG 
TCCTTGGCTT CGTTTAGGTC ATCGTCCCCG GCTCTTCAAG TCAGCACGCC AGTGGTTCGA 
TCTTCACCCA TTATGAGCAA GTCTCCTGCC ATACCGAAAA GTGATCTTCT AAATAGTTTG 
CTTACAACCA ACAAGGAGAC ACCTGAGCCG AACACAGATG TTATGTCCAC TTCCAGCTCG 
GATGACGACA AGGACAAACC TGCTCCTCAC CCCATACTGG CTGGAGTGCT TAAAAACAAC 
AACTTGGTCA AAAGCACTTC TTCCAGTTCG ATTTTGTCCG ACTCCGGCGC AAGTAACGTG 
TCTATGACGT CATCCATACG TACAAAGAAG GTTTCATTTC AAGACGACGT AACAGTTTGT 
GATGGTAATG GGATCAGTAC GTTTAGTAAC GGATTGCGAG AACCTGACGC TTCTGTAGCT 
GATGTATCGT TCGATAGGTC GATGGAACAA TCGTTATCTG TCGACACGCA TGAGGGTGCG 
GAGAATGAGC CAAAGCAGTG GCGACCGCCC TCTAACGGTC CGCCTGCTCG AAATGATTTC 
CAACAGGTGG GCTACAGAGC AAGACCCGCA ACACCACACA GGTATGAACC ACCACCATAC 
CAACCCTCCC CTCTTGCATA CCAAACCAAC TCTCCTCGAT CGCACCAATC AGCTGTGGCC 
GCTGCTATAC AAAGCATAAG TGGGAATGCG GAAACAACAA GCAGTGCTGC AAGGTACATT 
CCAAGCCAAC CTGAACATTT CTCGGTATCT TGCGCATCAC CTGTGCCAGT AGTAACCAGG 
CAGGGGCACG AGGCTCCACA CAAGGAGGAA TGGAATCCTC TCCGACGACA ATCCGCAGAC 
GCAAGCTCTA ACTCACCTTC AATTACACAG CGGACATGTC CTACCCCTGT GCCAACCGCC 
CCGCATAAGT TAGCTGAGGT ACAAGTAGAA AACGAGCCTT CATATTTCCA AACAGAGCGA 
GCGCCATCTC GTTCTGAAAC ATTTAACTCC GACACACCCC CCTACTACGA ATGCTGCGAA 
ACTACGACGC CCGCCTCTGG CTCAATTACA CCAAGTCAAT TAAGAGATGC GTTAAAGACA 
CAGGACGAAC AGTATCAACA TCCAGTCGTT GAATCTGCGG GCCCTGAGAA TCAAAGTTCT 
GTCACATTTT CCTCTCTCCC TTTATCTGTC CCACCATCAG GGAACGTTTT GCCACAACCC 
GAAACCGATG TTACGAACAA GAAGGTCATG GCTATGGAGC GCCAACTAGC GAGTTTAACC 
GGCATGGTTG AATCCATTAT AACCAGTACC GACGGTGTTC AAACCAACAA GCCTGAACCC 
CCATACCAGA ATCAACCAGT CGGTGGGAAT CTCCCTAAAA CGTCACACAC CGTCCAACAA 
GTGATGACGT CATCAGAGAT GGGACAACAA CTCTTGAAGT TGAGGGAAAC AGCTCATAAT 
CTGAGAGATC AAATGACAGA TCTGAAACGA CAGCAGAGGA TAAACATGGA ACACGTGAAC 
GACATTGTGA GCGAGAAATC CCGGGAACTG GCTCAGTTTA TCGCTGTCAC AGCAGGCGCC 
CAGCACCGAC CGTTGCGTAT ACGAAGATTC CGACTTGACC AGCAGAGACA CGCTTACTCC 
GAGGATGCTA CGACCACGGA ACGAATGCTG TCGGAGGTGG AAGCCGCCGT AGAAGAACTA 
AGGAATGAAG TCGTGGAAAG AAGGTGCAGA GTTAACGCTC GTGAAGTCGA AGCTCTTGTT 
TTGCAACTCA GTCAAGCTGG AAGGACGGTA GCAAAGCTGA AAGGTTCATA TCCTGCTCTA 
TGTGAAGAGA TACGAACCGT AGGAGCAGGG GAAACGGAAG TCATTTACGC TGAAGAGAAG 
TTCGTTGAAG CGGAACCGAC ACGGTTGGAT GAACAGCTGA AACGATGTCG GCAACTTACT 
GCAACACTTT TCACTCTGAA AAAGTTGGCT ACAGTTCAAG AAGCGCGCAG TAAAACTCCG 
ACTTCATTCG GGGCAATTCG TCCGAACACA CCGAGTCAAC TCGGGGCTCG AAGAGGTGAA 
GATCCGAATG CGAGCCGAGG AAGAATGCTC GAGGAAGTTC GGAGGACTCG GGTCGATCCT 
GTAAGAAGAA TGGAGAGCAT TCGGGATGCT GAGGTTAGTT GGATACGAAG GCGAAAGTAC 
AACAGTGAAT ACGACTCGAA AAAATTCGAG AAGGCACTGG AGTTGGCTGA GAAGGCACTT 
AATCGAACCA ACCATCTTTC ACTGATCGAC CAACGTCGTC ATAACCACTT AATGGACCTG 
TACAAGAATC GTGACGTCAT GAACCACACA CCACGTAGCA ATCTCACAAC TTCGGATTCG 
AGTTCCATTG AATCGTTACC CCCTCCTCCG CCCCCTATGG ATCATATGGG TCATCAATTC 
CACCCTAATC AACAACCGAG CTATCCATTA CACCTCCCCC CTCCCCCAGC AGACCTTAAC 
CTTCCCGGCC CACCACCACA CGAGTATTCT GTCATCTCCC CCGACATCGG GTATATGAGC 
TCGGATCTCT CCCCGGAGCA TCGTGGCCAA ACACAGACCG GCTCACTACC GAGGTATTCA 
AACGCCACGC GGTTTCATGG GTATCAGCAG CTTGAACGAA GCCATGGCAC CAGCCACCCT 
GCTTATGTGG ATTACCACAA TCATGGCCGC CAGACGCCAC AGATGGTTGA AAGGTCACGA 
TCGGTGACGC CACACCACTG GAACCAGCGT CCCATCACGC CACAGATGTT TACAAGCGAG 
ATACCAGTCG GACGCGAGTT GACAGAAGAT CAGCGACGTC ATATATTGGA GCAACAACAT 
CTAATGAACG AAGAGAAGAA TTGCAAGTTA AAAGAACAAT ATTCAAAGCT GCAACAACTA 
CAACGGAAGC AGAGAATGGC AAGCAAGAAA TATGTACCGA AGCCGAACTA TCAGTCTATG 
TCGATGGCGG CTTCAGCAAA ATCGTCCGAT GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018668726.1
CDS	1..5496
Translation	MDENTQEAGDSEVENDKASYAMLRPLQKVIAHFEMKLAANKGNIPTPRREIPNLKPQAERLRQSRKTSKQRSFIKSSGKLLSSVQEKVTVGSKSSTPCHSRSSSVCSSNVESHVPTSEGPPMTSSQQDFDTTSVSSTSSMTSETVDSQVKYSSLQRNYQHGHKSFQFNTLDGRRTRSKSGGHHNRSFYPQDGSISPAEADRKRSAFFQYLVQKYPQHSDVIVGKSFQQTQTPAPSFGGIRTRASYAADCSYNSSSPNDSEVTWHNSLSRGSRLRSSLPIVRSLSRSRERPLGTIYLEYSGECRTARLPNEVTSLDTIRALFVQAYPGLLTMPMLDPQRHSILLKNRKTGNYDEIDNVSRIKDRSFIRTRPIGGSGIPLPGTKKPTINHPKPAAKPVKPIPGNNRSKPRVTDDVKIAERNQKNLNKSGIKAPVKSLIRQYQSAPSLIKQAVTGSNEIKPVQSGEETQGKVKPPPPPRSAKPTVVAQTEAAQDQGNYSPNGTLLSGNGIGGGSIPQKTFSYVKSGNESPVNHQQQTITSPPQHRGLRQPTTSRLSPPSVEKKFSSIPTPGSKPKIQLPPNPTKPTDDEHHTKENVPRPKTPNILHQSPPTVTKLPPTSFAMTSPKNGAQRLPQPKPFGMKQHTTPANRFGAKRTSPPNVPNNNQLVPETAPVYSNIKRSASPERRATINGTGGSAGYCRGGRSFKGHQLKPVNIPSVPDTEIAAASNLEAKPDLNVPEVAQPVEPRQPISLPSDTKYSAMLPNPCTTDQVTSCSSSTSSDDVTDPGKSKMSTKKGLFGLRRLVSNRNKNPQNIPKRSQSEERPKTSDPSVNKSDLHSPSFNESLASFRSSSPALQVSTPVVRSSPIMSKSPAIPKSDLLNSLLTTNKETPEPNTDVMSTSSSDDDKDKPAPHPILAGVLKNNNLVKSTSSSSILSDSGASNVSMTSSIRTKKVSFQDDVTVCDGNGISTFSNGLREPDASVADVSFDRSMEQSLSVDTHEGAENEPKQWRPPSNGPPARNDFQQVGYRARPATPHRYEPPPYQPSPLAYQTNSPRSHQSAVAAAIQSISGNAETTSSAARYIPSQPEHFSVSCASPVPVVTRQGHEAPHKEEWNPLRRQSADASSNSPSITQRTCPTPVPTAPHKLAEVQVENEPSYFQTERAPSRSETFNSDTPPYYECCETTTPASGSITPSQLRDALKTQDEQYQHPVVESAGPENQSSVTFSSLPLSVPPSGNVLPQPETDVTNKKVMAMERQLASLTGMVESIITSTDGVQTNKPEPPYQNQPVGGNLPKTSHTVQQVMTSSEMGQQLLKLRETAHNLRDQMTDLKRQQRINMEHVNDIVSEKSRELAQFIAVTAGAQHRPLRIRRFRLDQQRHAYSEDATTTERMLSEVEAAVEELRNEVVERRCRVNAREVEALVLQLSQAGRTVAKLKGSYPALCEEIRTVGAGETEVIYAEEKFVEAEPTRLDEQLKRCRQLTATLFTLKKLATVQEARSKTPTSFGAIRPNTPSQLGARRGEDPNASRGRMLEEVRRTRVDPVRRMESIRDAEVSWIRRRKYNSEYDSKKFEKALELAEKALNRTNHLSLIDQRRHNHLMDLYKNRDVMNHTPRSNLTTSDSSSIESLPPPPPPMDHMGHQFHPNQQPSYPLHLPPPPADLNLPGPPPHEYSVISPDIGYMSSDLSPEHRGQTQTGSLPRYSNATRFHGYQQLERSHGTSHPAYVDYHNHGRQTPQMVERSRSVTPHHWNQRPITPQMFTSEIPVGRELTEDQRRHILEQQHLMNEEKNCKLKEQYSKLQQLQRKQRMASKKYVPKPNYQSMSMAASAKSSDV

Target ORF information:

RefSeq Version	XM_018813181.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis uncharacterized LOC100185798 (LOC100185798), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018813181.1

ORF Insert Sequence:

ATGGATGAAA ATACACAAGA AGCTGGCGAC TCAGAAGTCG AAAATGACAA GGCAAGTTAC 
GCGATGTTGA GACCCTTGCA GAAAGTGATC GCTCACTTTG AGATGAAACT TGCCGCCAAC 
AAAGGGAACA TTCCAACACC CAGACGAGAA ATACCAAACC TGAAACCGCA AGCTGAGCGT 
CTTCGTCAGA GCAGGAAAAC GTCCAAACAA CGAAGTTTTA TTAAAAGCAG TGGGAAGCTA 
TTGTCAAGCG TGCAAGAGAA GGTTACTGTT GGATCTAAAT CGTCAACACC TTGTCATAGC 
AGATCAAGTT CGGTGTGTTC AAGTAATGTG GAAAGTCATG TACCTACGTC AGAAGGCCCT 
CCCATGACAT CATCGCAACA AGATTTTGAC ACCACTTCTG TTTCGTCTAC GTCCAGTATG 
ACGTCAGAAA CTGTAGATTC TCAAGTTAAA TATTCCTCGT TACAAAGAAA CTACCAACAT 
GGGCACAAGA GTTTTCAGTT CAACACTCTG GATGGAAGGA GAACTCGATC TAAGAGTGGA 
GGCCACCATA ATCGAAGTTT CTATCCGCAA GATGGCAGTA TATCCCCAGC CGAAGCAGAT 
CGAAAGCGCT CAGCCTTTTT TCAATATTTA GTCCAAAAAT ACCCGCAACA CTCCGATGTT 
ATTGTTGGGA AATCATTTCA GCAAACCCAG ACTCCAGCAC CTTCCTTCGG TGGGATTCGA 
ACCCGTGCAA GCTACGCTGC CGATTGCTCT TATAACTCTT CATCTCCTAA CGACAGCGAG 
GTCACGTGGC ATAACAGTTT GTCACGTGGT AGTAGACTCA GGTCCAGCCT CCCAATCGTA 
AGAAGTTTAA GTCGAAGCCG AGAACGACCA TTAGGCACCA TTTACTTGGA GTATAGCGGA 
GAGTGTAGAA CTGCCAGACT GCCGAACGAA GTGACAAGCC TTGATACAAT ACGAGCTCTC 
TTCGTACAAG CATACCCAGG ATTGCTAACG ATGCCAATGC TTGACCCACA ACGTCACTCC 
ATTTTATTAA AAAACAGGAA GACGGGAAAC TACGATGAGA TCGATAACGT GAGTCGCATC 
AAGGATCGTT CATTCATTCG AACTCGTCCG ATCGGGGGTT CAGGTATTCC ATTGCCTGGG 
ACCAAGAAGC CTACAATCAA CCACCCGAAG CCTGCTGCTA AACCAGTTAA GCCAATACCG 
GGCAACAACC GATCAAAACC ACGGGTTACT GATGATGTAA AAATTGCAGA ACGCAACCAA 
AAGAACTTGA ACAAAAGTGG GATAAAAGCA CCGGTTAAAT CCCTGATCCG TCAATACCAG 
TCTGCACCTA GTTTAATAAA GCAAGCAGTG ACCGGTTCAA ACGAGATAAA ACCTGTTCAA 
AGTGGCGAGG AGACTCAGGG TAAAGTGAAA CCGCCTCCCC CACCGCGGTC CGCAAAGCCA 
ACAGTCGTAG CACAAACAGA AGCAGCCCAA GATCAAGGCA ATTATTCGCC TAACGGGACT 
CTATTATCAG GCAATGGGAT TGGGGGTGGT AGTATACCAC AGAAAACTTT CTCGTACGTT 
AAAAGCGGCA ATGAGTCTCC AGTAAACCAC CAACAACAGA CAATCACATC CCCACCGCAG 
CATAGGGGCC TGAGGCAACC TACAACTTCC AGGCTTTCCC CTCCATCTGT TGAGAAGAAG 
TTTTCCTCCA TACCAACGCC AGGGTCAAAG CCGAAGATCC AACTCCCTCC GAATCCAACA 
AAACCAACCG ATGACGAACA CCACACTAAG GAGAACGTGC CACGCCCAAA GACTCCCAAC 
ATACTCCACC AATCACCGCC CACCGTCACC AAACTTCCGC CTACGTCATT CGCAATGACG 
TCACCGAAAA ATGGCGCTCA ACGCCTTCCC CAACCAAAAC CTTTCGGCAT GAAACAACAT 
ACAACGCCAG CGAACCGGTT CGGTGCAAAA CGAACGTCCC CTCCAAACGT CCCAAACAAT 
AATCAATTGG TGCCGGAAAC TGCGCCGGTC TATTCAAATA TAAAGCGGTC AGCCTCACCG 
GAAAGAAGAG CTACAATAAA CGGTACCGGT GGATCAGCAG GTTACTGCAG GGGTGGTAGG 
TCCTTTAAAG GCCACCAGCT GAAACCTGTA AATATTCCAT CCGTTCCCGA CACTGAAATA 
GCTGCAGCTT CTAACCTGGA AGCCAAACCT GATCTCAACG TGCCGGAGGT AGCTCAACCT 
GTAGAACCGC GGCAACCCAT TTCATTACCC AGTGATACCA AATACTCTGC CATGCTACCG 
AACCCTTGTA CCACAGATCA AGTGACGTCA TGCAGCTCCT CTACGTCATC CGATGACGTC 
ACCGACCCTG GAAAATCAAA GATGTCAACG AAGAAGGGAT TGTTCGGGCT TCGGAGATTG 
GTTTCGAACC GAAACAAGAA CCCACAAAAT ATTCCGAAAC GAAGTCAAAG CGAGGAGCGA 
CCGAAGACAT CGGATCCTTC GGTAAATAAA TCCGACCTTC ATTCTCCATC TTTCAACGAG 
TCCTTGGCTT CGTTTAGGTC ATCGTCCCCG GCTCTTCAAG TCAGCACGCC AGTGGTTCGA 
TCTTCACCCA TTATGAGCAA GTCTCCTGCC ATACCGAAAA GTGATCTTCT AAATAGTTTG 
CTTACAACCA ACAAGGAGAC ACCTGAGCCG AACACAGATG TTATGTCCAC TTCCAGCTCG 
GATGACGACA AGGACAAACC TGCTCCTCAC CCCATACTGG CTGGAGTGCT TAAAAACAAC 
AACTTGGTCA AAAGCACTTC TTCCAGTTCG ATTTTGTCCG ACTCCGGCGC AAGTAACGTG 
TCTATGACGT CATCCATACG TACAAAGAAG GTTTCATTTC AAGACGACGT AACAGTTTGT 
GATGGTAATG GGATCAGTAC GTTTAGTAAC GGATTGCGAG AACCTGACGC TTCTGTAGCT 
GATGTATCGT TCGATAGGTC GATGGAACAA TCGTTATCTG TCGACACGCA TGAGGGTGCG 
GAGAATGAGC CAAAGCAGTG GCGACCGCCC TCTAACGGTC CGCCTGCTCG AAATGATTTC 
CAACAGGTGG GCTACAGAGC AAGACCCGCA ACACCACACA GGTATGAACC ACCACCATAC 
CAACCCTCCC CTCTTGCATA CCAAACCAAC TCTCCTCGAT CGCACCAATC AGCTGTGGCC 
GCTGCTATAC AAAGCATAAG TGGGAATGCG GAAACAACAA GCAGTGCTGC AAGGTACATT 
CCAAGCCAAC CTGAACATTT CTCGGTATCT TGCGCATCAC CTGTGCCAGT AGTAACCAGG 
CAGGGGCACG AGGCTCCACA CAAGGAGGAA TGGAATCCTC TCCGACGACA ATCCGCAGAC 
GCAAGCTCTA ACTCACCTTC AATTACACAG CGGACATGTC CTACCCCTGT GCCAACCGCC 
CCGCATAAGT TAGCTGAGGT ACAAGTAGAA AACGAGCCTT CATATTTCCA AACAGAGCGA 
GCGCCATCTC GTTCTGAAAC ATTTAACTCC GACACACCCC CCTACTACGA ATGCTGCGAA 
ACTACGACGC CCGCCTCTGG CTCAATTACA CCAAGTCAAT TAAGAGATGC GTTAAAGACA 
CAGGACGAAC AGTATCAACA TCCAGTCGTT GAATCTGCGG GCCCTGAGAA TCAAAGTTCT 
GTCACATTTT CCTCTCTCCC TTTATCTGTC CCACCATCAG GGAACGTTTT GCCACAACCC 
GAAACCGATG TTACGAACAA GAAGGTCATG GCTATGGAGC GCCAACTAGC GAGTTTAACC 
GGCATGGTTG AATCCATTAT AACCAGTACC GACGGTGTTC AAACCAACAA GCCTGAACCC 
CCATACCAGA ATCAACCAGT CGGTGGGAAT CTCCCTAAAA CGTCACACAC CGTCCAACAA 
GTGATGACGT CATCAGAGAT GGGACAACAA CTCTTGAAGT TGAGGGAAAC AGCTCATAAT 
CTGAGAGATC AAATGACAGA TCTGAAACGA CAGCAGAGGA TAAACATGGA ACACGTGAAC 
GACATTGTGA GCGAGAAATC CCGGGAACTG GCTCAGTTTA TCGCTGTCAC AGCAGGCGCC 
CAGCACCGAC CGTTGCGTAT ACGAAGATTC CGACTTGACC AGCAGAGACA CGCTTACTCC 
GAGGATGCTA CGACCACGGA ACGAATGCTG TCGGAGGTGG AAGCCGCCGT AGAAGAACTA 
AGGAATGAAG TCGTGGAAAG AAGGTGCAGA GTTAACGCTC GTGAAGTCGA AGCTCTTGTT 
TTGCAACTCA GTCAAGCTGG AAGGACGGTA GCAAAGCTGA AAGGTTCATA TCCTGCTCTA 
TGTGAAGAGA TACGAACCGT AGGAGCAGGG GAAACGGAAG TCATTTACGC TGAAGAGAAG 
TTCGTTGAAG CGGAACCGAC ACGGTTGGAT GAACAGCTGA AACGATGTCG GCAACTTACT 
GCAACACTTT TCACTCTGAA AAAGTTGGCT ACAGTTCAAG AAGCGCGCAG TAAAACTCCG 
ACTTCATTCG GGGCAATTCG TCCGAACACA CCGAGTCAAC TCGGGGCTCG AAGAGGTGAA 
GATCCGAATG CGAGCCGAGG AAGAATGCTC GAGGAAGTTC GGAGGACTCG GGTCGATCCT 
GTAAGAAGAA TGGAGAGCAT TCGGGATGCT GAGGTTAGTT GGATACGAAG GCGAAAGTAC 
AACAGTGAAT ACGACTCGAA AAAATTCGAG AAGGCACTGG AGTTGGCTGA GAAGGCACTT 
AATCGAACCA ACCATCTTTC ACTGATCGAC CAACGTCGTC ATAACCACTT AATGGACCTG 
TACAAGAATC GTGACGTCAT GAACCACACA CCACGTAGCA ATCTCACAAC TTCGGATTCG 
AGTTCCATTG AATCGTTACC CCCTCCTCCG CCCCCTATGG ATCATATGGG TCATCAATTC 
CACCCTAATC AACAACCGAG CTATCCATTA CACCTCCCCC CTCCCCCAGC AGACCTTAAC 
CTTCCCGGCC CACCACCACA CGAGTATTCT GTCATCTCCC CCGACATCGG GTATATGAGC 
TCGGATCTCT CCCCGGAGCA TCGTGGCCAA ACACAGACCG GCTCACTACC GAGGTATTCA 
AACGCCACGC GGTTTCATGG GTATCAGCAG CTTGAACGAA GCCATGGCAC CAGCCACCCT 
GCTTATGTGG ATTACCACAA TCATGGCCGC CAGACGCCAC AGATGGTTGA AAGGTCACGA 
TCGGTGACGC CACACCACTG GAACCAGCGT CCCATCACGC CACAGATGTT TACAAGCGAG 
ATACCAGTCG GACGCGAGTT GACAGAAGAT CAGCGACGTC ATATATTGGA GCAACAACAT 
CTAATGAACG AAGAGAAGAA TTGCAAGTTA AAAGAACAAT ATTCAAAGCT GCAACAACTA 
CAACGGAAGC AGAGAATGGC AAGCAAGAAA TATGTACCGA AGCCGAACTA TCAGTCTATG 
TCGATGGCGG CTTCAGCAAA ATCGTCCGAT GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100185798 ( XM_018813181.2 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_018668726.1 Ciona intestinalis coiled-coil domain-containing protein CG32809 (LOC100185798), transcript variant X1, mRNA.

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CloneID	OCi74452
Clone ID Related Accession (Same CDS sequence)	XM_018813181.1 , XM_018813181.2
Accession Version	XM_018813181.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1540051200000
Organism	Ciona intestinalis(vase tunicate)
Product	uncharacterized protein LOC100185798 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020174.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 22, 2018 this sequence version replaced XM_018813181.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ciona intestinalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAA ATACACAAGA AGCTGGCGAC TCAGAAGTCG AAAATGACAA GGCAAGTTAC 
GCGATGTTGA GACCCTTGCA GAAAGTGATC GCTCACTTTG AGATGAAACT TGCCGCCAAC 
AAAGGGAACA TTCCAACACC CAGACGAGAA ATACCAAACC TGAAACCGCA AGCTGAGCGT 
CTTCGTCAGA GCAGGAAAAC GTCCAAACAA CGAAGTTTTA TTAAAAGCAG TGGGAAGCTA 
TTGTCAAGCG TGCAAGAGAA GGTTACTGTT GGATCTAAAT CGTCAACACC TTGTCATAGC 
AGATCAAGTT CGGTGTGTTC AAGTAATGTG GAAAGTCATG TACCTACGTC AGAAGGCCCT 
CCCATGACAT CATCGCAACA AGATTTTGAC ACCACTTCTG TTTCGTCTAC GTCCAGTATG 
ACGTCAGAAA CTGTAGATTC TCAAGTTAAA TATTCCTCGT TACAAAGAAA CTACCAACAT 
GGGCACAAGA GTTTTCAGTT CAACACTCTG GATGGAAGGA GAACTCGATC TAAGAGTGGA 
GGCCACCATA ATCGAAGTTT CTATCCGCAA GATGGCAGTA TATCCCCAGC CGAAGCAGAT 
CGAAAGCGCT CAGCCTTTTT TCAATATTTA GTCCAAAAAT ACCCGCAACA CTCCGATGTT 
ATTGTTGGGA AATCATTTCA GCAAACCCAG ACTCCAGCAC CTTCCTTCGG TGGGATTCGA 
ACCCGTGCAA GCTACGCTGC CGATTGCTCT TATAACTCTT CATCTCCTAA CGACAGCGAG 
GTCACGTGGC ATAACAGTTT GTCACGTGGT AGTAGACTCA GGTCCAGCCT CCCAATCGTA 
AGAAGTTTAA GTCGAAGCCG AGAACGACCA TTAGGCACCA TTTACTTGGA GTATAGCGGA 
GAGTGTAGAA CTGCCAGACT GCCGAACGAA GTGACAAGCC TTGATACAAT ACGAGCTCTC 
TTCGTACAAG CATACCCAGG ATTGCTAACG ATGCCAATGC TTGACCCACA ACGTCACTCC 
ATTTTATTAA AAAACAGGAA GACGGGAAAC TACGATGAGA TCGATAACGT GAGTCGCATC 
AAGGATCGTT CATTCATTCG AACTCGTCCG ATCGGGGGTT CAGGTATTCC ATTGCCTGGG 
ACCAAGAAGC CTACAATCAA CCACCCGAAG CCTGCTGCTA AACCAGTTAA GCCAATACCG 
GGCAACAACC GATCAAAACC ACGGGTTACT GATGATGTAA AAATTGCAGA ACGCAACCAA 
AAGAACTTGA ACAAAAGTGG GATAAAAGCA CCGGTTAAAT CCCTGATCCG TCAATACCAG 
TCTGCACCTA GTTTAATAAA GCAAGCAGTG ACCGGTTCAA ACGAGATAAA ACCTGTTCAA 
AGTGGCGAGG AGACTCAGGG TAAAGTGAAA CCGCCTCCCC CACCGCGGTC CGCAAAGCCA 
ACAGTCGTAG CACAAACAGA AGCAGCCCAA GATCAAGGCA ATTATTCGCC TAACGGGACT 
CTATTATCAG GCAATGGGAT TGGGGGTGGT AGTATACCAC AGAAAACTTT CTCGTACGTT 
AAAAGCGGCA ATGAGTCTCC AGTAAACCAC CAACAACAGA CAATCACATC CCCACCGCAG 
CATAGGGGCC TGAGGCAACC TACAACTTCC AGGCTTTCCC CTCCATCTGT TGAGAAGAAG 
TTTTCCTCCA TACCAACGCC AGGGTCAAAG CCGAAGATCC AACTCCCTCC GAATCCAACA 
AAACCAACCG ATGACGAACA CCACACTAAG GAGAACGTGC CACGCCCAAA GACTCCCAAC 
ATACTCCACC AATCACCGCC CACCGTCACC AAACTTCCGC CTACGTCATT CGCAATGACG 
TCACCGAAAA ATGGCGCTCA ACGCCTTCCC CAACCAAAAC CTTTCGGCAT GAAACAACAT 
ACAACGCCAG CGAACCGGTT CGGTGCAAAA CGAACGTCCC CTCCAAACGT CCCAAACAAT 
AATCAATTGG TGCCGGAAAC TGCGCCGGTC TATTCAAATA TAAAGCGGTC AGCCTCACCG 
GAAAGAAGAG CTACAATAAA CGGTACCGGT GGATCAGCAG GTTACTGCAG GGGTGGTAGG 
TCCTTTAAAG GCCACCAGCT GAAACCTGTA AATATTCCAT CCGTTCCCGA CACTGAAATA 
GCTGCAGCTT CTAACCTGGA AGCCAAACCT GATCTCAACG TGCCGGAGGT AGCTCAACCT 
GTAGAACCGC GGCAACCCAT TTCATTACCC AGTGATACCA AATACTCTGC CATGCTACCG 
AACCCTTGTA CCACAGATCA AGTGACGTCA TGCAGCTCCT CTACGTCATC CGATGACGTC 
ACCGACCCTG GAAAATCAAA GATGTCAACG AAGAAGGGAT TGTTCGGGCT TCGGAGATTG 
GTTTCGAACC GAAACAAGAA CCCACAAAAT ATTCCGAAAC GAAGTCAAAG CGAGGAGCGA 
CCGAAGACAT CGGATCCTTC GGTAAATAAA TCCGACCTTC ATTCTCCATC TTTCAACGAG 
TCCTTGGCTT CGTTTAGGTC ATCGTCCCCG GCTCTTCAAG TCAGCACGCC AGTGGTTCGA 
TCTTCACCCA TTATGAGCAA GTCTCCTGCC ATACCGAAAA GTGATCTTCT AAATAGTTTG 
CTTACAACCA ACAAGGAGAC ACCTGAGCCG AACACAGATG TTATGTCCAC TTCCAGCTCG 
GATGACGACA AGGACAAACC TGCTCCTCAC CCCATACTGG CTGGAGTGCT TAAAAACAAC 
AACTTGGTCA AAAGCACTTC TTCCAGTTCG ATTTTGTCCG ACTCCGGCGC AAGTAACGTG 
TCTATGACGT CATCCATACG TACAAAGAAG GTTTCATTTC AAGACGACGT AACAGTTTGT 
GATGGTAATG GGATCAGTAC GTTTAGTAAC GGATTGCGAG AACCTGACGC TTCTGTAGCT 
GATGTATCGT TCGATAGGTC GATGGAACAA TCGTTATCTG TCGACACGCA TGAGGGTGCG 
GAGAATGAGC CAAAGCAGTG GCGACCGCCC TCTAACGGTC CGCCTGCTCG AAATGATTTC 
CAACAGGTGG GCTACAGAGC AAGACCCGCA ACACCACACA GGTATGAACC ACCACCATAC 
CAACCCTCCC CTCTTGCATA CCAAACCAAC TCTCCTCGAT CGCACCAATC AGCTGTGGCC 
GCTGCTATAC AAAGCATAAG TGGGAATGCG GAAACAACAA GCAGTGCTGC AAGGTACATT 
CCAAGCCAAC CTGAACATTT CTCGGTATCT TGCGCATCAC CTGTGCCAGT AGTAACCAGG 
CAGGGGCACG AGGCTCCACA CAAGGAGGAA TGGAATCCTC TCCGACGACA ATCCGCAGAC 
GCAAGCTCTA ACTCACCTTC AATTACACAG CGGACATGTC CTACCCCTGT GCCAACCGCC 
CCGCATAAGT TAGCTGAGGT ACAAGTAGAA AACGAGCCTT CATATTTCCA AACAGAGCGA 
GCGCCATCTC GTTCTGAAAC ATTTAACTCC GACACACCCC CCTACTACGA ATGCTGCGAA 
ACTACGACGC CCGCCTCTGG CTCAATTACA CCAAGTCAAT TAAGAGATGC GTTAAAGACA 
CAGGACGAAC AGTATCAACA TCCAGTCGTT GAATCTGCGG GCCCTGAGAA TCAAAGTTCT 
GTCACATTTT CCTCTCTCCC TTTATCTGTC CCACCATCAG GGAACGTTTT GCCACAACCC 
GAAACCGATG TTACGAACAA GAAGGTCATG GCTATGGAGC GCCAACTAGC GAGTTTAACC 
GGCATGGTTG AATCCATTAT AACCAGTACC GACGGTGTTC AAACCAACAA GCCTGAACCC 
CCATACCAGA ATCAACCAGT CGGTGGGAAT CTCCCTAAAA CGTCACACAC CGTCCAACAA 
GTGATGACGT CATCAGAGAT GGGACAACAA CTCTTGAAGT TGAGGGAAAC AGCTCATAAT 
CTGAGAGATC AAATGACAGA TCTGAAACGA CAGCAGAGGA TAAACATGGA ACACGTGAAC 
GACATTGTGA GCGAGAAATC CCGGGAACTG GCTCAGTTTA TCGCTGTCAC AGCAGGCGCC 
CAGCACCGAC CGTTGCGTAT ACGAAGATTC CGACTTGACC AGCAGAGACA CGCTTACTCC 
GAGGATGCTA CGACCACGGA ACGAATGCTG TCGGAGGTGG AAGCCGCCGT AGAAGAACTA 
AGGAATGAAG TCGTGGAAAG AAGGTGCAGA GTTAACGCTC GTGAAGTCGA AGCTCTTGTT 
TTGCAACTCA GTCAAGCTGG AAGGACGGTA GCAAAGCTGA AAGGTTCATA TCCTGCTCTA 
TGTGAAGAGA TACGAACCGT AGGAGCAGGG GAAACGGAAG TCATTTACGC TGAAGAGAAG 
TTCGTTGAAG CGGAACCGAC ACGGTTGGAT GAACAGCTGA AACGATGTCG GCAACTTACT 
GCAACACTTT TCACTCTGAA AAAGTTGGCT ACAGTTCAAG AAGCGCGCAG TAAAACTCCG 
ACTTCATTCG GGGCAATTCG TCCGAACACA CCGAGTCAAC TCGGGGCTCG AAGAGGTGAA 
GATCCGAATG CGAGCCGAGG AAGAATGCTC GAGGAAGTTC GGAGGACTCG GGTCGATCCT 
GTAAGAAGAA TGGAGAGCAT TCGGGATGCT GAGGTTAGTT GGATACGAAG GCGAAAGTAC 
AACAGTGAAT ACGACTCGAA AAAATTCGAG AAGGCACTGG AGTTGGCTGA GAAGGCACTT 
AATCGAACCA ACCATCTTTC ACTGATCGAC CAACGTCGTC ATAACCACTT AATGGACCTG 
TACAAGAATC GTGACGTCAT GAACCACACA CCACGTAGCA ATCTCACAAC TTCGGATTCG 
AGTTCCATTG AATCGTTACC CCCTCCTCCG CCCCCTATGG ATCATATGGG TCATCAATTC 
CACCCTAATC AACAACCGAG CTATCCATTA CACCTCCCCC CTCCCCCAGC AGACCTTAAC 
CTTCCCGGCC CACCACCACA CGAGTATTCT GTCATCTCCC CCGACATCGG GTATATGAGC 
TCGGATCTCT CCCCGGAGCA TCGTGGCCAA ACACAGACCG GCTCACTACC GAGGTATTCA 
AACGCCACGC GGTTTCATGG GTATCAGCAG CTTGAACGAA GCCATGGCAC CAGCCACCCT 
GCTTATGTGG ATTACCACAA TCATGGCCGC CAGACGCCAC AGATGGTTGA AAGGTCACGA 
TCGGTGACGC CACACCACTG GAACCAGCGT CCCATCACGC CACAGATGTT TACAAGCGAG 
ATACCAGTCG GACGCGAGTT GACAGAAGAT CAGCGACGTC ATATATTGGA GCAACAACAT 
CTAATGAACG AAGAGAAGAA TTGCAAGTTA AAAGAACAAT ATTCAAAGCT GCAACAACTA 
CAACGGAAGC AGAGAATGGC AAGCAAGAAA TATGTACCGA AGCCGAACTA TCAGTCTATG 
TCGATGGCGG CTTCAGCAAA ATCGTCCGAT GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018668726.1
CDS	16..5511
Translation	MDENTQEAGDSEVENDKASYAMLRPLQKVIAHFEMKLAANKGNIPTPRREIPNLKPQAERLRQSRKTSKQRSFIKSSGKLLSSVQEKVTVGSKSSTPCHSRSSSVCSSNVESHVPTSEGPPMTSSQQDFDTTSVSSTSSMTSETVDSQVKYSSLQRNYQHGHKSFQFNTLDGRRTRSKSGGHHNRSFYPQDGSISPAEADRKRSAFFQYLVQKYPQHSDVIVGKSFQQTQTPAPSFGGIRTRASYAADCSYNSSSPNDSEVTWHNSLSRGSRLRSSLPIVRSLSRSRERPLGTIYLEYSGECRTARLPNEVTSLDTIRALFVQAYPGLLTMPMLDPQRHSILLKNRKTGNYDEIDNVSRIKDRSFIRTRPIGGSGIPLPGTKKPTINHPKPAAKPVKPIPGNNRSKPRVTDDVKIAERNQKNLNKSGIKAPVKSLIRQYQSAPSLIKQAVTGSNEIKPVQSGEETQGKVKPPPPPRSAKPTVVAQTEAAQDQGNYSPNGTLLSGNGIGGGSIPQKTFSYVKSGNESPVNHQQQTITSPPQHRGLRQPTTSRLSPPSVEKKFSSIPTPGSKPKIQLPPNPTKPTDDEHHTKENVPRPKTPNILHQSPPTVTKLPPTSFAMTSPKNGAQRLPQPKPFGMKQHTTPANRFGAKRTSPPNVPNNNQLVPETAPVYSNIKRSASPERRATINGTGGSAGYCRGGRSFKGHQLKPVNIPSVPDTEIAAASNLEAKPDLNVPEVAQPVEPRQPISLPSDTKYSAMLPNPCTTDQVTSCSSSTSSDDVTDPGKSKMSTKKGLFGLRRLVSNRNKNPQNIPKRSQSEERPKTSDPSVNKSDLHSPSFNESLASFRSSSPALQVSTPVVRSSPIMSKSPAIPKSDLLNSLLTTNKETPEPNTDVMSTSSSDDDKDKPAPHPILAGVLKNNNLVKSTSSSSILSDSGASNVSMTSSIRTKKVSFQDDVTVCDGNGISTFSNGLREPDASVADVSFDRSMEQSLSVDTHEGAENEPKQWRPPSNGPPARNDFQQVGYRARPATPHRYEPPPYQPSPLAYQTNSPRSHQSAVAAAIQSISGNAETTSSAARYIPSQPEHFSVSCASPVPVVTRQGHEAPHKEEWNPLRRQSADASSNSPSITQRTCPTPVPTAPHKLAEVQVENEPSYFQTERAPSRSETFNSDTPPYYECCETTTPASGSITPSQLRDALKTQDEQYQHPVVESAGPENQSSVTFSSLPLSVPPSGNVLPQPETDVTNKKVMAMERQLASLTGMVESIITSTDGVQTNKPEPPYQNQPVGGNLPKTSHTVQQVMTSSEMGQQLLKLRETAHNLRDQMTDLKRQQRINMEHVNDIVSEKSRELAQFIAVTAGAQHRPLRIRRFRLDQQRHAYSEDATTTERMLSEVEAAVEELRNEVVERRCRVNAREVEALVLQLSQAGRTVAKLKGSYPALCEEIRTVGAGETEVIYAEEKFVEAEPTRLDEQLKRCRQLTATLFTLKKLATVQEARSKTPTSFGAIRPNTPSQLGARRGEDPNASRGRMLEEVRRTRVDPVRRMESIRDAEVSWIRRRKYNSEYDSKKFEKALELAEKALNRTNHLSLIDQRRHNHLMDLYKNRDVMNHTPRSNLTTSDSSSIESLPPPPPPMDHMGHQFHPNQQPSYPLHLPPPPADLNLPGPPPHEYSVISPDIGYMSSDLSPEHRGQTQTGSLPRYSNATRFHGYQQLERSHGTSHPAYVDYHNHGRQTPQMVERSRSVTPHHWNQRPITPQMFTSEIPVGRELTEDQRRHILEQQHLMNEEKNCKLKEQYSKLQQLQRKQRMASKKYVPKPNYQSMSMAASAKSSDV

Target ORF information:

RefSeq Version	XM_018813181.2
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis coiled-coil domain-containing protein CG32809 (LOC100185798), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018813181.2

ORF Insert Sequence:

ATGGATGAAA ATACACAAGA AGCTGGCGAC TCAGAAGTCG AAAATGACAA GGCAAGTTAC 
GCGATGTTGA GACCCTTGCA GAAAGTGATC GCTCACTTTG AGATGAAACT TGCCGCCAAC 
AAAGGGAACA TTCCAACACC CAGACGAGAA ATACCAAACC TGAAACCGCA AGCTGAGCGT 
CTTCGTCAGA GCAGGAAAAC GTCCAAACAA CGAAGTTTTA TTAAAAGCAG TGGGAAGCTA 
TTGTCAAGCG TGCAAGAGAA GGTTACTGTT GGATCTAAAT CGTCAACACC TTGTCATAGC 
AGATCAAGTT CGGTGTGTTC AAGTAATGTG GAAAGTCATG TACCTACGTC AGAAGGCCCT 
CCCATGACAT CATCGCAACA AGATTTTGAC ACCACTTCTG TTTCGTCTAC GTCCAGTATG 
ACGTCAGAAA CTGTAGATTC TCAAGTTAAA TATTCCTCGT TACAAAGAAA CTACCAACAT 
GGGCACAAGA GTTTTCAGTT CAACACTCTG GATGGAAGGA GAACTCGATC TAAGAGTGGA 
GGCCACCATA ATCGAAGTTT CTATCCGCAA GATGGCAGTA TATCCCCAGC CGAAGCAGAT 
CGAAAGCGCT CAGCCTTTTT TCAATATTTA GTCCAAAAAT ACCCGCAACA CTCCGATGTT 
ATTGTTGGGA AATCATTTCA GCAAACCCAG ACTCCAGCAC CTTCCTTCGG TGGGATTCGA 
ACCCGTGCAA GCTACGCTGC CGATTGCTCT TATAACTCTT CATCTCCTAA CGACAGCGAG 
GTCACGTGGC ATAACAGTTT GTCACGTGGT AGTAGACTCA GGTCCAGCCT CCCAATCGTA 
AGAAGTTTAA GTCGAAGCCG AGAACGACCA TTAGGCACCA TTTACTTGGA GTATAGCGGA 
GAGTGTAGAA CTGCCAGACT GCCGAACGAA GTGACAAGCC TTGATACAAT ACGAGCTCTC 
TTCGTACAAG CATACCCAGG ATTGCTAACG ATGCCAATGC TTGACCCACA ACGTCACTCC 
ATTTTATTAA AAAACAGGAA GACGGGAAAC TACGATGAGA TCGATAACGT GAGTCGCATC 
AAGGATCGTT CATTCATTCG AACTCGTCCG ATCGGGGGTT CAGGTATTCC ATTGCCTGGG 
ACCAAGAAGC CTACAATCAA CCACCCGAAG CCTGCTGCTA AACCAGTTAA GCCAATACCG 
GGCAACAACC GATCAAAACC ACGGGTTACT GATGATGTAA AAATTGCAGA ACGCAACCAA 
AAGAACTTGA ACAAAAGTGG GATAAAAGCA CCGGTTAAAT CCCTGATCCG TCAATACCAG 
TCTGCACCTA GTTTAATAAA GCAAGCAGTG ACCGGTTCAA ACGAGATAAA ACCTGTTCAA 
AGTGGCGAGG AGACTCAGGG TAAAGTGAAA CCGCCTCCCC CACCGCGGTC CGCAAAGCCA 
ACAGTCGTAG CACAAACAGA AGCAGCCCAA GATCAAGGCA ATTATTCGCC TAACGGGACT 
CTATTATCAG GCAATGGGAT TGGGGGTGGT AGTATACCAC AGAAAACTTT CTCGTACGTT 
AAAAGCGGCA ATGAGTCTCC AGTAAACCAC CAACAACAGA CAATCACATC CCCACCGCAG 
CATAGGGGCC TGAGGCAACC TACAACTTCC AGGCTTTCCC CTCCATCTGT TGAGAAGAAG 
TTTTCCTCCA TACCAACGCC AGGGTCAAAG CCGAAGATCC AACTCCCTCC GAATCCAACA 
AAACCAACCG ATGACGAACA CCACACTAAG GAGAACGTGC CACGCCCAAA GACTCCCAAC 
ATACTCCACC AATCACCGCC CACCGTCACC AAACTTCCGC CTACGTCATT CGCAATGACG 
TCACCGAAAA ATGGCGCTCA ACGCCTTCCC CAACCAAAAC CTTTCGGCAT GAAACAACAT 
ACAACGCCAG CGAACCGGTT CGGTGCAAAA CGAACGTCCC CTCCAAACGT CCCAAACAAT 
AATCAATTGG TGCCGGAAAC TGCGCCGGTC TATTCAAATA TAAAGCGGTC AGCCTCACCG 
GAAAGAAGAG CTACAATAAA CGGTACCGGT GGATCAGCAG GTTACTGCAG GGGTGGTAGG 
TCCTTTAAAG GCCACCAGCT GAAACCTGTA AATATTCCAT CCGTTCCCGA CACTGAAATA 
GCTGCAGCTT CTAACCTGGA AGCCAAACCT GATCTCAACG TGCCGGAGGT AGCTCAACCT 
GTAGAACCGC GGCAACCCAT TTCATTACCC AGTGATACCA AATACTCTGC CATGCTACCG 
AACCCTTGTA CCACAGATCA AGTGACGTCA TGCAGCTCCT CTACGTCATC CGATGACGTC 
ACCGACCCTG GAAAATCAAA GATGTCAACG AAGAAGGGAT TGTTCGGGCT TCGGAGATTG 
GTTTCGAACC GAAACAAGAA CCCACAAAAT ATTCCGAAAC GAAGTCAAAG CGAGGAGCGA 
CCGAAGACAT CGGATCCTTC GGTAAATAAA TCCGACCTTC ATTCTCCATC TTTCAACGAG 
TCCTTGGCTT CGTTTAGGTC ATCGTCCCCG GCTCTTCAAG TCAGCACGCC AGTGGTTCGA 
TCTTCACCCA TTATGAGCAA GTCTCCTGCC ATACCGAAAA GTGATCTTCT AAATAGTTTG 
CTTACAACCA ACAAGGAGAC ACCTGAGCCG AACACAGATG TTATGTCCAC TTCCAGCTCG 
GATGACGACA AGGACAAACC TGCTCCTCAC CCCATACTGG CTGGAGTGCT TAAAAACAAC 
AACTTGGTCA AAAGCACTTC TTCCAGTTCG ATTTTGTCCG ACTCCGGCGC AAGTAACGTG 
TCTATGACGT CATCCATACG TACAAAGAAG GTTTCATTTC AAGACGACGT AACAGTTTGT 
GATGGTAATG GGATCAGTAC GTTTAGTAAC GGATTGCGAG AACCTGACGC TTCTGTAGCT 
GATGTATCGT TCGATAGGTC GATGGAACAA TCGTTATCTG TCGACACGCA TGAGGGTGCG 
GAGAATGAGC CAAAGCAGTG GCGACCGCCC TCTAACGGTC CGCCTGCTCG AAATGATTTC 
CAACAGGTGG GCTACAGAGC AAGACCCGCA ACACCACACA GGTATGAACC ACCACCATAC 
CAACCCTCCC CTCTTGCATA CCAAACCAAC TCTCCTCGAT CGCACCAATC AGCTGTGGCC 
GCTGCTATAC AAAGCATAAG TGGGAATGCG GAAACAACAA GCAGTGCTGC AAGGTACATT 
CCAAGCCAAC CTGAACATTT CTCGGTATCT TGCGCATCAC CTGTGCCAGT AGTAACCAGG 
CAGGGGCACG AGGCTCCACA CAAGGAGGAA TGGAATCCTC TCCGACGACA ATCCGCAGAC 
GCAAGCTCTA ACTCACCTTC AATTACACAG CGGACATGTC CTACCCCTGT GCCAACCGCC 
CCGCATAAGT TAGCTGAGGT ACAAGTAGAA AACGAGCCTT CATATTTCCA AACAGAGCGA 
GCGCCATCTC GTTCTGAAAC ATTTAACTCC GACACACCCC CCTACTACGA ATGCTGCGAA 
ACTACGACGC CCGCCTCTGG CTCAATTACA CCAAGTCAAT TAAGAGATGC GTTAAAGACA 
CAGGACGAAC AGTATCAACA TCCAGTCGTT GAATCTGCGG GCCCTGAGAA TCAAAGTTCT 
GTCACATTTT CCTCTCTCCC TTTATCTGTC CCACCATCAG GGAACGTTTT GCCACAACCC 
GAAACCGATG TTACGAACAA GAAGGTCATG GCTATGGAGC GCCAACTAGC GAGTTTAACC 
GGCATGGTTG AATCCATTAT AACCAGTACC GACGGTGTTC AAACCAACAA GCCTGAACCC 
CCATACCAGA ATCAACCAGT CGGTGGGAAT CTCCCTAAAA CGTCACACAC CGTCCAACAA 
GTGATGACGT CATCAGAGAT GGGACAACAA CTCTTGAAGT TGAGGGAAAC AGCTCATAAT 
CTGAGAGATC AAATGACAGA TCTGAAACGA CAGCAGAGGA TAAACATGGA ACACGTGAAC 
GACATTGTGA GCGAGAAATC CCGGGAACTG GCTCAGTTTA TCGCTGTCAC AGCAGGCGCC 
CAGCACCGAC CGTTGCGTAT ACGAAGATTC CGACTTGACC AGCAGAGACA CGCTTACTCC 
GAGGATGCTA CGACCACGGA ACGAATGCTG TCGGAGGTGG AAGCCGCCGT AGAAGAACTA 
AGGAATGAAG TCGTGGAAAG AAGGTGCAGA GTTAACGCTC GTGAAGTCGA AGCTCTTGTT 
TTGCAACTCA GTCAAGCTGG AAGGACGGTA GCAAAGCTGA AAGGTTCATA TCCTGCTCTA 
TGTGAAGAGA TACGAACCGT AGGAGCAGGG GAAACGGAAG TCATTTACGC TGAAGAGAAG 
TTCGTTGAAG CGGAACCGAC ACGGTTGGAT GAACAGCTGA AACGATGTCG GCAACTTACT 
GCAACACTTT TCACTCTGAA AAAGTTGGCT ACAGTTCAAG AAGCGCGCAG TAAAACTCCG 
ACTTCATTCG GGGCAATTCG TCCGAACACA CCGAGTCAAC TCGGGGCTCG AAGAGGTGAA 
GATCCGAATG CGAGCCGAGG AAGAATGCTC GAGGAAGTTC GGAGGACTCG GGTCGATCCT 
GTAAGAAGAA TGGAGAGCAT TCGGGATGCT GAGGTTAGTT GGATACGAAG GCGAAAGTAC 
AACAGTGAAT ACGACTCGAA AAAATTCGAG AAGGCACTGG AGTTGGCTGA GAAGGCACTT 
AATCGAACCA ACCATCTTTC ACTGATCGAC CAACGTCGTC ATAACCACTT AATGGACCTG 
TACAAGAATC GTGACGTCAT GAACCACACA CCACGTAGCA ATCTCACAAC TTCGGATTCG 
AGTTCCATTG AATCGTTACC CCCTCCTCCG CCCCCTATGG ATCATATGGG TCATCAATTC 
CACCCTAATC AACAACCGAG CTATCCATTA CACCTCCCCC CTCCCCCAGC AGACCTTAAC 
CTTCCCGGCC CACCACCACA CGAGTATTCT GTCATCTCCC CCGACATCGG GTATATGAGC 
TCGGATCTCT CCCCGGAGCA TCGTGGCCAA ACACAGACCG GCTCACTACC GAGGTATTCA 
AACGCCACGC GGTTTCATGG GTATCAGCAG CTTGAACGAA GCCATGGCAC CAGCCACCCT 
GCTTATGTGG ATTACCACAA TCATGGCCGC CAGACGCCAC AGATGGTTGA AAGGTCACGA 
TCGGTGACGC CACACCACTG GAACCAGCGT CCCATCACGC CACAGATGTT TACAAGCGAG 
ATACCAGTCG GACGCGAGTT GACAGAAGAT CAGCGACGTC ATATATTGGA GCAACAACAT 
CTAATGAACG AAGAGAAGAA TTGCAAGTTA AAAGAACAAT ATTCAAAGCT GCAACAACTA 
CAACGGAAGC AGAGAATGGC AAGCAAGAAA TATGTACCGA AGCCGAACTA TCAGTCTATG 
TCGATGGCGG CTTCAGCAAA ATCGTCCGAT GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100185798 cDNA ORF clone, Ciona intestinalis(vase tunicate)

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Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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