BRAFLDRAFT_70615 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAFLDRAFT_70615
Entrez Gene ID	7221853
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Branchiostoma floridae(Florida lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002604720.1	XP_002604766.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Putnam NH, Butts T, Ferrier DE, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu JK, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, Toyoda A, Bronner-Fraser M, Fujiyama A, Holland LZ, Holland PW, Satoh N, Rokhsar DS
Nature453(7198)1064-71(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRAFLDRAFT_70615 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAFLDRAFT_70615 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBb21253	XM_002604720.1 Latest version!	Branchiostoma floridae hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAFLDRAFT_70615 ( XM_002604720.1 ) cDNA ORF clone, Branchiostoma floridae(Florida lancelet) -> XP_002604766.1 Branchiostoma floridae hypothetical protein, mRNA.

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CloneID	OBb21253
Clone ID Related Accession (Same CDS sequence)	XM_002604720.1
Accession Version	XM_002604720.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1255708800000
Organism	Branchiostoma floridae(Florida lancelet)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101511). COMPLETENESS: incomplete on both ends.

ATGACCATAT ACGAGCCGGA AGCTAGCGTC ATCGTGGAAT TTGTGTCTTG TTCGTATATT 
TTTCCATCCT ACAGAATCAT GATGAAGTTT GAACTTTTGA TGCTTGCCTT ACTGATCGCT 
GCGGTTTGTG CCGAGCCGTT TGATGACGTG AAGCAACCGC CGAAAGGTCC TTTACAAGGC 
CCAGAAGAGC CAGCCGATCG GAACGTGCGT GATGTGAGTG CCAGCGAAGG TGCCTGGCTG 
GATCGAAACT CTTCGTGGGT TGTGGACTCC GCCGGCACAC CGTGGGTAAA CAACGGAGTG 
CCGTATGATG CTGCAAAAAC CTTGGATGGG GACACTGGGA CCTACTGGAA CCCACTGGAT 
ACAGTTCAGT ACTACAACAA CTGGTACATC GTACTGGACC TCACGAAGCC TCATACTCTG 
TCCCGCATCG CAGTCAACAA CTACGGAGAC AACTACCATG ACATTGCAGC CTTCAAGTTG 
CAAAAGTCAC AGGTAGGGAG TCCGTACTGC TGGGAAGACG CCATGTCCGT TACTAACGTA 
CAGGGAGGGA CCAACCAGCG CCAGGAGTTC GACGGTTTCC AGGGAACAGC GCGGTACTGG 
AGGTTCCTGA TCACTCAGAC CATGTCAGGC TGGCAGCCGA TACTTATAGA ACTGGAGCTC 
TACGGATACT CATCAGCTGC AGCAGTTGGT TTGTGGCCCT TGAACGCACA GCATGGGGCA 
TCAGACATCA CCGGAAACGG AAATGACGGG GCAGCAACAG GAACCCAGCT AGTCCCAGGT 
CCGTACGGAG ACGCCGACGG AGCTTTCCTG TTTTCGGGAA CTACAAATTC CTACATCGAG 
ATTCCCAACA ACGGCAGGTT GGATGTGCGC TACTCCTTCA CCATACTGGT CCACATCTAT 
CCTACCGGAG AGTATGGACC GATCTTCGAC TATGTGGGGA ACAACAACTG GGCATTACAC 
TTTTGGCAGG TGTCTGCACA GCAGCTTTAC ATGCGGCCGG TAGGCAGAGA TGGACACAAT 
CCACCTGAGA TCATTGCGGA CGCGCTGCAG CGGAACGCCT GGAACTATGT GGGTGCAACC 
TACGACAGAG CCACGGGGAA GGCAGCCCTC TGGAGCAACG GCGAGCTGGT CCGTGAAACA 
CACATTCCGG TTGCCGAACT CGCAAGTCAG TACTTTGTCC GGGTGGGGGT GCGGGACGGA 
GATAGTCGGT ACTTCTCGGG TCGTGTCGCC TGTCTGCAGC TGTACGACTT CGCCATGAAT 
AAAGAACAAG TTGCGGCGGT GCGGGATGCG TGTAAAAAAG GTGATTGGCT GGATCGGAAC 
TCCTCCTGGG TTGTGGACTC CGCCGGTACA CCTTGGGTAA ACAACGGAGT GCCGTATGAT 
GCCGCAAAAA CCTTGGATGG GGACACCGGG ACCTGGTGGA ACCCACTGGA TACGGTTCAG 
TACTACAACA ACTGGTACAT CGTACTGGAC CTCACGGAGC CTCATACTCT GTCCCGCATC 
GCAGTCAACA ACTACGGAGA CAACTACCAT GACATTGTAG CCTTCATTTT GCAAAAGTCA 
CAGGTTGGGA GTCCGTACGA CTGGAAGAAC GTAGTAACCG TTACTAACGT ACAGGGAGGG 
ACCAACCAGC GGCAGGAGTT CGGCGGGTTC CAGGGAACAG CGCGGTACTG GAGGTTCCTG 
ATCACTCAGA CCATGTCAGG CTGGCAGCCG ATACTTATAG AACTGGAGCT CTACGGATAC 
TCATCAGTGA CGCCCCCACC TGAAGATCTT AGGATTACAT ACATCACAGA AACGTCCGTT 
ACAGCCTCGT GGAAGCAGAG GATTGACTCT CTCGCTCTCC ACCATAAGAT GTGGATAAGG 
CGCAGTGATA CTTTTGAGAG CCTGTTCACA CAGCTAGTGC CCACAGGTCA GACTGACCTG 
ACGTTCATTG ACCTCAACCC TGGCACAGAG TATGTGATAT CAGCCACAAG CATCAACAGG 
CAAAACGAGG GTCCAGCCGT GAACCTTACT GTTGGAACAA AAACGGACAG TCCTACAGCT 
TTAGGTGTTG CACAGAAGAC AACCAGCGCC ATAACAATCT CATGGACACC ACCTCAAGCT 
GTCCTTATCG CTTACAACAT AACTTACACA AACGAAAATG GAAGGAGTAC TTCCGGGATG 
ACGTCAGGGG ATGTAGACAG TTATGAACTG ACAGGTCTGG TTCCTGGTAC TCTGTATGAC 
ATAGACTTGG TGGCCGTTAG CAAGGTGGGG AGGAGCATCG CCGTCAGTAT AAGTGACGTA 
ACAGACACAG ATCCGCCGTC GAGTCTGAGA GCCACGGAGT CCTCCACGGT ATGGCTGTTC 
CTGGAATGGG CGCCACCTGT GGCAAACATC CTGTACTACA GCCTAGACAT TTCAGACGAA 
CATGGCGGAG ACGGAACGTT GCTAAGGGTT GATGGGCACC AAACGTTCTA TAACGTCACT 
GGTCTGGTAC CGGAAACTAC ATACGTCATC AAGATGGCGT CCTTCGGCGA GCATGGTCGT 
AGTGTGGACA TCACTTATTC AAATAGTACA GTAACTTCAG CAGGAAAAAC AACAAAGCCT 
CCCCTTATCG AGGAAATATC GAGGACAGCC TTGCTGCAGG CGGCTTCAGC AGTTATCGAT 
GTGTTACCGG TACCCGATCA GGAAACACAG GTCGCAAGTA CCCCCGGCCA TATGTTAGAA 
AGTGATCTTA TTGACACAAG TGATACTGAG CTTTCTCCCA AGCAACAGAT AAAGATGTTA 
GAAGAGAAAA AGAAGGAAAA AGAAGATATG AAGCCTACTG TGCTGACCCT GGGCAAGGAC 
GACTACACAC TGTTGATATC AGCTGCAGGC TGCTCGTCTT GGAAGGACAG TAAGAAACAA 
TGGAAGCTGG AGGGCTGTGA TGCTGACATT AATCTGGACA ATGGAGCCAT CAGCTGCCGG 
TGTCACATGA CTGAGGGGAA GGTCTCTGTG GGGACCATGA CTCTTCCTCT TCCCAACTCC 
ATCAACTTCA TCAACGCTTT CAGGAACTTC CGCAATCTGA GCGAAAACCC CGTAGTGTTC 
TCCATCGTGG TGTCCGAGTA CACCCTGTAT ATAATACTCA TGGTTTTCTT GTGCGTGGAC 
TTTCATCGTC TATGGATTTC TATTCGATAT CGTGTCACCT CTCGGATAAG CCCAATTACT 
GAAGATAATG AGGGAGCAAG CGACCAAAGG AAGCTGCTGC CCAAAGTGAG TCTTGTCCCT 
CCTGAAAGGA TGCCTGCACC ACACGTCTAT CAGATCACGG TCACCACAGG GTCCATGTTC 
AGCGCAGGCA CCGCATCACG TGTCGGCTTC CAACTGTACG GGTCAGAGGG CACATCTCCA 
GTCAAAATGC TGAACCCGAA AGGAGAGGCT TTGGTGCGAG GAAGTACTTT GCACTTTGTG 
ATGCCCGTGC GAGAGTCACT TGGAGAAGTG ATGTTGTTGC ACATCTGGCA CGATAACTCT 
GGGGAAGGCG ACAATTCATC GTGGTTCCTC GGAAGTTTTG TCGTCAGAGA TGTAGAAAAG 
GATGTGGTGT CTTACTTCAA CTGCAACGAC TGGTTATCAG CAGAAAAAGG GGACGGTGAG 
GTGCAGAAGG TGGTTCACGC TAGCACCGAG GAGGAGCTGA CATCATTTAC CAATGTCTTT 
ATTGAGGCAA CCAAAAATGC GTTCTACGAT AAACAGCTGT GGGCGTCTGT CCTTGTAGCA 
GCACCGGGAT CCAGCTTCAC CAAAGCCCAG CGTCTGTCCT GTTGCTTTAC TCTCCTGAAC 
ACCATGATGC TGGCCAGTGC CATGTGGTAC AGGGAAGAAA ACACAACTGC TGACACAAGA 
GTGTTCAACC TGGGGTTTGT TCGTTTTACA ATAGAGATCT TTGTGCTTGC TGTGGCCCTG 
GCGTCCATCC TTAGCCTCCC GCTCCTCGCC AAGCCGCCGA CTATTCGGAA GGAAGACTTG 
CAGCTGAACC TGTGGAACAC AACAGCTCCG AAGAAAGTCC ACCCACCTGC CAAGCCTAAT 
AGGCAGTCTG CGAGGAAAAA TAAGGAGCTG AATGAGAAAT CAGCCTCAGT ACTGAAGGAG 
ATTCTTCTCC TGTTGGTCTT CGTATCCCTT CTCTTCTACA TCGCCCAGGC AGACAAAGAC 
CAACAAAAGA CTTTCTATGA AACCCAGTCT TTATCGAAGA ATATTCTGCA AAACTATGAT 
GTGATCAGAA CTCCGGACAA GTTTTACATA TGGGCTGAGG ACGTCCTGCT GCGGACTCTT 
TACCCGGCCA CCTGGTACAA CGGAAAGGAC ATGAAGTACT TAGACCGGCA GTTTGCACAC 
AACACGGGAT CCTTCCGCCT CGGTCCTCCT CGTCTTACTC AAGTTAGACA TCTTCCGGGT 
GAGCTGGGAT TTGAGCGCTT TATTGACCTG CAAACTGCTG CATATTGGGA TGCTTGTTTC 
AAGCACATGT TGGGTCTGGT AGTCTTCATC AACACCATTT CCTTACTTCG CGTCGTCCGC 
TTCAGTAAAC CAATCGGCAA ACTCCTGGCC CTTCCCGGTA TCATGAAGAA GGAGCTCCTG 
GCGTTCGTAG TTTCTGCTGT CGTGGCCTTC ATGGCCTTCA TCAGCTCAGG ATTCCTCATA 
TTTGGAAGCG AAATGGAATC TTACACAGAC CTGTACCAAA CGACGTTTGC ACTCTTCGAG 
ATGATGCTTG GAAGGTTCTT CGCCCAGGAA ATGTTGGATT CCAATCCTAT CACTGGACCA 
ATCTTCTTCT CCACCTTCAT GATCTGCATC TTCATCCTCC TGGTGAACTT CCTGATGACC 
ATCATCTGTG ACGCAATTTC CGCTGACGTT GACGTCGACC ATGACCGTGA ACTTGCCGAT 
CACATGTGGA GGAGCTTCTG GACTATGATG GGGTTTCACA GCACGCCAAA AAAGGAAGAT 
AAACCAGATG ATTTGAAGAT AGAAGAGCTG AAGGCGAACA TTTGCATCAT TCGGGAGGTA 
CTTGATGAAG GTCTGGACAT CTGCAACTCC ATCCTGCCTC CGCATCGACA GGAAAAACAC 
CCGAAGAACT TAGAAGAACC CTGCTTCTTG AAGCATGACC CCTGTAACGC TTCTTACCAA 
ATAATTCAGA AAGAACTTGA GTTAGAAACG ATTGTCGAAG AAATTCAAGG CAGCACTAGA 
GGCACGGAGT TTTCTCGACA AGCAGCTGAT ACAGACATCG AAAACGTGAT GCACGAATAT 
GAGAAAGAAG AGAAAACCCA CCAGGAGCAT AAACTTTTAG CCGAGGTAAG GATGCAGACC 
AAGAAAGTTG AGAACATCCT TTTGAGGGTA AATCGCAGTA CCACTAACAA AACTGTGCAG 
AAGGTGCATC AACGTATTCA GGTTGCAGCA GCTCAAAGAG AGAAGCAGAA TACGACTACT 
GCCCTGCATA ATGATGAACA GAGACATTTA CGAAACATGT GGATCCCCGA TCTAAAGCTC 
ACCGCTGAAG ACAAGGTCGT GCTCCTTCGT GATGAGATGC TGACAGACAA ACACATCCAT 
GCCGCACAGA TGTTACTCCG CCGTCAGTAT CCAGGGCTGG GAGGACTACA AGATACGGCA 
GTAGGAGCGT CCGTGTACGG ATATACACGG GTCTCCGGAG ACGGTCTACA GATTCACCAC 
ACCGGATCCC TTCACTGGGT GGTCTCTTCC TCCATAGGTG GACATGTCAG CGTTTATAAC 
AGCATGCCCA GTAAAATAAA TGCGTCACTG GAGTATCAGT TGTGTCAGTG TTACGCCCCA 
CCTCCCAACA GGAACACAGG CGTACTGGCT GTGAAAGTAC CACGTGTACA GCACCAGGGG 
AGCAGGTATA GCTGCGGACT GTTTGCCATA GCCTGGGCTG TAGACATCGC AATGGGGACG 
GATGTGACTC AGGTCAGGTA CAAGGAGAGC AGGATGAGAG CCCATCTCCG GGACTGCTTC 
GAGAGCGGTT ACCTGTCTCC CTTCCCAGGG GCCCGTTGTA TCACCAGTCA GCGACCCAGC 
ACTGAGCACC GGATCACTCT TGCTGCCGGC AAAGGCACCC ATTCAGTTGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002604766.1
CDS	1..6054
Translation	MTIYEPEASVIVEFVSCSYIFPSYRIMMKFELLMLALLIAAVCAEPFDDVKQPPKGPLQGPEEPADRNVRDVSASEGAWLDRNSSWVVDSAGTPWVNNGVPYDAAKTLDGDTGTYWNPLDTVQYYNNWYIVLDLTKPHTLSRIAVNNYGDNYHDIAAFKLQKSQVGSPYCWEDAMSVTNVQGGTNQRQEFDGFQGTARYWRFLITQTMSGWQPILIELELYGYSSAAAVGLWPLNAQHGASDITGNGNDGAATGTQLVPGPYGDADGAFLFSGTTNSYIEIPNNGRLDVRYSFTILVHIYPTGEYGPIFDYVGNNNWALHFWQVSAQQLYMRPVGRDGHNPPEIIADALQRNAWNYVGATYDRATGKAALWSNGELVRETHIPVAELASQYFVRVGVRDGDSRYFSGRVACLQLYDFAMNKEQVAAVRDACKKGDWLDRNSSWVVDSAGTPWVNNGVPYDAAKTLDGDTGTWWNPLDTVQYYNNWYIVLDLTEPHTLSRIAVNNYGDNYHDIVAFILQKSQVGSPYDWKNVVTVTNVQGGTNQRQEFGGFQGTARYWRFLITQTMSGWQPILIELELYGYSSVTPPPEDLRITYITETSVTASWKQRIDSLALHHKMWIRRSDTFESLFTQLVPTGQTDLTFIDLNPGTEYVISATSINRQNEGPAVNLTVGTKTDSPTALGVAQKTTSAITISWTPPQAVLIAYNITYTNENGRSTSGMTSGDVDSYELTGLVPGTLYDIDLVAVSKVGRSIAVSISDVTDTDPPSSLRATESSTVWLFLEWAPPVANILYYSLDISDEHGGDGTLLRVDGHQTFYNVTGLVPETTYVIKMASFGEHGRSVDITYSNSTVTSAGKTTKPPLIEEISRTALLQAASAVIDVLPVPDQETQVASTPGHMLESDLIDTSDTELSPKQQIKMLEEKKKEKEDMKPTVLTLGKDDYTLLISAAGCSSWKDSKKQWKLEGCDADINLDNGAISCRCHMTEGKVSVGTMTLPLPNSINFINAFRNFRNLSENPVVFSIVVSEYTLYIILMVFLCVDFHRLWISIRYRVTSRISPITEDNEGASDQRKLLPKVSLVPPERMPAPHVYQITVTTGSMFSAGTASRVGFQLYGSEGTSPVKMLNPKGEALVRGSTLHFVMPVRESLGEVMLLHIWHDNSGEGDNSSWFLGSFVVRDVEKDVVSYFNCNDWLSAEKGDGEVQKVVHASTEEELTSFTNVFIEATKNAFYDKQLWASVLVAAPGSSFTKAQRLSCCFTLLNTMMLASAMWYREENTTADTRVFNLGFVRFTIEIFVLAVALASILSLPLLAKPPTIRKEDLQLNLWNTTAPKKVHPPAKPNRQSARKNKELNEKSASVLKEILLLLVFVSLLFYIAQADKDQQKTFYETQSLSKNILQNYDVIRTPDKFYIWAEDVLLRTLYPATWYNGKDMKYLDRQFAHNTGSFRLGPPRLTQVRHLPGELGFERFIDLQTAAYWDACFKHMLGLVVFINTISLLRVVRFSKPIGKLLALPGIMKKELLAFVVSAVVAFMAFISSGFLIFGSEMESYTDLYQTTFALFEMMLGRFFAQEMLDSNPITGPIFFSTFMICIFILLVNFLMTIICDAISADVDVDHDRELADHMWRSFWTMMGFHSTPKKEDKPDDLKIEELKANICIIREVLDEGLDICNSILPPHRQEKHPKNLEEPCFLKHDPCNASYQIIQKELELETIVEEIQGSTRGTEFSRQAADTDIENVMHEYEKEEKTHQEHKLLAEVRMQTKKVENILLRVNRSTTNKTVQKVHQRIQVAAAQREKQNTTTALHNDEQRHLRNMWIPDLKLTAEDKVVLLRDEMLTDKHIHAAQMLLRRQYPGLGGLQDTAVGASVYGYTRVSGDGLQIHHTGSLHWVVSSSIGGHVSVYNSMPSKINASLEYQLCQCYAPPPNRNTGVLAVKVPRVQHQGSRYSCGLFAIAWAVDIAMGTDVTQVRYKESRMRAHLRDCFESGYLSPFPGARCITSQRPSTEHRITLAAGKGTHSVE

Target ORF information:

RefSeq Version	XM_002604720.1
Organism	Branchiostoma floridae(Florida lancelet)
Definition	Branchiostoma floridae hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002604720.1

ORF Insert Sequence:

ATGACCATAT ACGAGCCGGA AGCTAGCGTC ATCGTGGAAT TTGTGTCTTG TTCGTATATT 
TTTCCATCCT ACAGAATCAT GATGAAGTTT GAACTTTTGA TGCTTGCCTT ACTGATCGCT 
GCGGTTTGTG CCGAGCCGTT TGATGACGTG AAGCAACCGC CGAAAGGTCC TTTACAAGGC 
CCAGAAGAGC CAGCCGATCG GAACGTGCGT GATGTGAGTG CCAGCGAAGG TGCCTGGCTG 
GATCGAAACT CTTCGTGGGT TGTGGACTCC GCCGGCACAC CGTGGGTAAA CAACGGAGTG 
CCGTATGATG CTGCAAAAAC CTTGGATGGG GACACTGGGA CCTACTGGAA CCCACTGGAT 
ACAGTTCAGT ACTACAACAA CTGGTACATC GTACTGGACC TCACGAAGCC TCATACTCTG 
TCCCGCATCG CAGTCAACAA CTACGGAGAC AACTACCATG ACATTGCAGC CTTCAAGTTG 
CAAAAGTCAC AGGTAGGGAG TCCGTACTGC TGGGAAGACG CCATGTCCGT TACTAACGTA 
CAGGGAGGGA CCAACCAGCG CCAGGAGTTC GACGGTTTCC AGGGAACAGC GCGGTACTGG 
AGGTTCCTGA TCACTCAGAC CATGTCAGGC TGGCAGCCGA TACTTATAGA ACTGGAGCTC 
TACGGATACT CATCAGCTGC AGCAGTTGGT TTGTGGCCCT TGAACGCACA GCATGGGGCA 
TCAGACATCA CCGGAAACGG AAATGACGGG GCAGCAACAG GAACCCAGCT AGTCCCAGGT 
CCGTACGGAG ACGCCGACGG AGCTTTCCTG TTTTCGGGAA CTACAAATTC CTACATCGAG 
ATTCCCAACA ACGGCAGGTT GGATGTGCGC TACTCCTTCA CCATACTGGT CCACATCTAT 
CCTACCGGAG AGTATGGACC GATCTTCGAC TATGTGGGGA ACAACAACTG GGCATTACAC 
TTTTGGCAGG TGTCTGCACA GCAGCTTTAC ATGCGGCCGG TAGGCAGAGA TGGACACAAT 
CCACCTGAGA TCATTGCGGA CGCGCTGCAG CGGAACGCCT GGAACTATGT GGGTGCAACC 
TACGACAGAG CCACGGGGAA GGCAGCCCTC TGGAGCAACG GCGAGCTGGT CCGTGAAACA 
CACATTCCGG TTGCCGAACT CGCAAGTCAG TACTTTGTCC GGGTGGGGGT GCGGGACGGA 
GATAGTCGGT ACTTCTCGGG TCGTGTCGCC TGTCTGCAGC TGTACGACTT CGCCATGAAT 
AAAGAACAAG TTGCGGCGGT GCGGGATGCG TGTAAAAAAG GTGATTGGCT GGATCGGAAC 
TCCTCCTGGG TTGTGGACTC CGCCGGTACA CCTTGGGTAA ACAACGGAGT GCCGTATGAT 
GCCGCAAAAA CCTTGGATGG GGACACCGGG ACCTGGTGGA ACCCACTGGA TACGGTTCAG 
TACTACAACA ACTGGTACAT CGTACTGGAC CTCACGGAGC CTCATACTCT GTCCCGCATC 
GCAGTCAACA ACTACGGAGA CAACTACCAT GACATTGTAG CCTTCATTTT GCAAAAGTCA 
CAGGTTGGGA GTCCGTACGA CTGGAAGAAC GTAGTAACCG TTACTAACGT ACAGGGAGGG 
ACCAACCAGC GGCAGGAGTT CGGCGGGTTC CAGGGAACAG CGCGGTACTG GAGGTTCCTG 
ATCACTCAGA CCATGTCAGG CTGGCAGCCG ATACTTATAG AACTGGAGCT CTACGGATAC 
TCATCAGTGA CGCCCCCACC TGAAGATCTT AGGATTACAT ACATCACAGA AACGTCCGTT 
ACAGCCTCGT GGAAGCAGAG GATTGACTCT CTCGCTCTCC ACCATAAGAT GTGGATAAGG 
CGCAGTGATA CTTTTGAGAG CCTGTTCACA CAGCTAGTGC CCACAGGTCA GACTGACCTG 
ACGTTCATTG ACCTCAACCC TGGCACAGAG TATGTGATAT CAGCCACAAG CATCAACAGG 
CAAAACGAGG GTCCAGCCGT GAACCTTACT GTTGGAACAA AAACGGACAG TCCTACAGCT 
TTAGGTGTTG CACAGAAGAC AACCAGCGCC ATAACAATCT CATGGACACC ACCTCAAGCT 
GTCCTTATCG CTTACAACAT AACTTACACA AACGAAAATG GAAGGAGTAC TTCCGGGATG 
ACGTCAGGGG ATGTAGACAG TTATGAACTG ACAGGTCTGG TTCCTGGTAC TCTGTATGAC 
ATAGACTTGG TGGCCGTTAG CAAGGTGGGG AGGAGCATCG CCGTCAGTAT AAGTGACGTA 
ACAGACACAG ATCCGCCGTC GAGTCTGAGA GCCACGGAGT CCTCCACGGT ATGGCTGTTC 
CTGGAATGGG CGCCACCTGT GGCAAACATC CTGTACTACA GCCTAGACAT TTCAGACGAA 
CATGGCGGAG ACGGAACGTT GCTAAGGGTT GATGGGCACC AAACGTTCTA TAACGTCACT 
GGTCTGGTAC CGGAAACTAC ATACGTCATC AAGATGGCGT CCTTCGGCGA GCATGGTCGT 
AGTGTGGACA TCACTTATTC AAATAGTACA GTAACTTCAG CAGGAAAAAC AACAAAGCCT 
CCCCTTATCG AGGAAATATC GAGGACAGCC TTGCTGCAGG CGGCTTCAGC AGTTATCGAT 
GTGTTACCGG TACCCGATCA GGAAACACAG GTCGCAAGTA CCCCCGGCCA TATGTTAGAA 
AGTGATCTTA TTGACACAAG TGATACTGAG CTTTCTCCCA AGCAACAGAT AAAGATGTTA 
GAAGAGAAAA AGAAGGAAAA AGAAGATATG AAGCCTACTG TGCTGACCCT GGGCAAGGAC 
GACTACACAC TGTTGATATC AGCTGCAGGC TGCTCGTCTT GGAAGGACAG TAAGAAACAA 
TGGAAGCTGG AGGGCTGTGA TGCTGACATT AATCTGGACA ATGGAGCCAT CAGCTGCCGG 
TGTCACATGA CTGAGGGGAA GGTCTCTGTG GGGACCATGA CTCTTCCTCT TCCCAACTCC 
ATCAACTTCA TCAACGCTTT CAGGAACTTC CGCAATCTGA GCGAAAACCC CGTAGTGTTC 
TCCATCGTGG TGTCCGAGTA CACCCTGTAT ATAATACTCA TGGTTTTCTT GTGCGTGGAC 
TTTCATCGTC TATGGATTTC TATTCGATAT CGTGTCACCT CTCGGATAAG CCCAATTACT 
GAAGATAATG AGGGAGCAAG CGACCAAAGG AAGCTGCTGC CCAAAGTGAG TCTTGTCCCT 
CCTGAAAGGA TGCCTGCACC ACACGTCTAT CAGATCACGG TCACCACAGG GTCCATGTTC 
AGCGCAGGCA CCGCATCACG TGTCGGCTTC CAACTGTACG GGTCAGAGGG CACATCTCCA 
GTCAAAATGC TGAACCCGAA AGGAGAGGCT TTGGTGCGAG GAAGTACTTT GCACTTTGTG 
ATGCCCGTGC GAGAGTCACT TGGAGAAGTG ATGTTGTTGC ACATCTGGCA CGATAACTCT 
GGGGAAGGCG ACAATTCATC GTGGTTCCTC GGAAGTTTTG TCGTCAGAGA TGTAGAAAAG 
GATGTGGTGT CTTACTTCAA CTGCAACGAC TGGTTATCAG CAGAAAAAGG GGACGGTGAG 
GTGCAGAAGG TGGTTCACGC TAGCACCGAG GAGGAGCTGA CATCATTTAC CAATGTCTTT 
ATTGAGGCAA CCAAAAATGC GTTCTACGAT AAACAGCTGT GGGCGTCTGT CCTTGTAGCA 
GCACCGGGAT CCAGCTTCAC CAAAGCCCAG CGTCTGTCCT GTTGCTTTAC TCTCCTGAAC 
ACCATGATGC TGGCCAGTGC CATGTGGTAC AGGGAAGAAA ACACAACTGC TGACACAAGA 
GTGTTCAACC TGGGGTTTGT TCGTTTTACA ATAGAGATCT TTGTGCTTGC TGTGGCCCTG 
GCGTCCATCC TTAGCCTCCC GCTCCTCGCC AAGCCGCCGA CTATTCGGAA GGAAGACTTG 
CAGCTGAACC TGTGGAACAC AACAGCTCCG AAGAAAGTCC ACCCACCTGC CAAGCCTAAT 
AGGCAGTCTG CGAGGAAAAA TAAGGAGCTG AATGAGAAAT CAGCCTCAGT ACTGAAGGAG 
ATTCTTCTCC TGTTGGTCTT CGTATCCCTT CTCTTCTACA TCGCCCAGGC AGACAAAGAC 
CAACAAAAGA CTTTCTATGA AACCCAGTCT TTATCGAAGA ATATTCTGCA AAACTATGAT 
GTGATCAGAA CTCCGGACAA GTTTTACATA TGGGCTGAGG ACGTCCTGCT GCGGACTCTT 
TACCCGGCCA CCTGGTACAA CGGAAAGGAC ATGAAGTACT TAGACCGGCA GTTTGCACAC 
AACACGGGAT CCTTCCGCCT CGGTCCTCCT CGTCTTACTC AAGTTAGACA TCTTCCGGGT 
GAGCTGGGAT TTGAGCGCTT TATTGACCTG CAAACTGCTG CATATTGGGA TGCTTGTTTC 
AAGCACATGT TGGGTCTGGT AGTCTTCATC AACACCATTT CCTTACTTCG CGTCGTCCGC 
TTCAGTAAAC CAATCGGCAA ACTCCTGGCC CTTCCCGGTA TCATGAAGAA GGAGCTCCTG 
GCGTTCGTAG TTTCTGCTGT CGTGGCCTTC ATGGCCTTCA TCAGCTCAGG ATTCCTCATA 
TTTGGAAGCG AAATGGAATC TTACACAGAC CTGTACCAAA CGACGTTTGC ACTCTTCGAG 
ATGATGCTTG GAAGGTTCTT CGCCCAGGAA ATGTTGGATT CCAATCCTAT CACTGGACCA 
ATCTTCTTCT CCACCTTCAT GATCTGCATC TTCATCCTCC TGGTGAACTT CCTGATGACC 
ATCATCTGTG ACGCAATTTC CGCTGACGTT GACGTCGACC ATGACCGTGA ACTTGCCGAT 
CACATGTGGA GGAGCTTCTG GACTATGATG GGGTTTCACA GCACGCCAAA AAAGGAAGAT 
AAACCAGATG ATTTGAAGAT AGAAGAGCTG AAGGCGAACA TTTGCATCAT TCGGGAGGTA 
CTTGATGAAG GTCTGGACAT CTGCAACTCC ATCCTGCCTC CGCATCGACA GGAAAAACAC 
CCGAAGAACT TAGAAGAACC CTGCTTCTTG AAGCATGACC CCTGTAACGC TTCTTACCAA 
ATAATTCAGA AAGAACTTGA GTTAGAAACG ATTGTCGAAG AAATTCAAGG CAGCACTAGA 
GGCACGGAGT TTTCTCGACA AGCAGCTGAT ACAGACATCG AAAACGTGAT GCACGAATAT 
GAGAAAGAAG AGAAAACCCA CCAGGAGCAT AAACTTTTAG CCGAGGTAAG GATGCAGACC 
AAGAAAGTTG AGAACATCCT TTTGAGGGTA AATCGCAGTA CCACTAACAA AACTGTGCAG 
AAGGTGCATC AACGTATTCA GGTTGCAGCA GCTCAAAGAG AGAAGCAGAA TACGACTACT 
GCCCTGCATA ATGATGAACA GAGACATTTA CGAAACATGT GGATCCCCGA TCTAAAGCTC 
ACCGCTGAAG ACAAGGTCGT GCTCCTTCGT GATGAGATGC TGACAGACAA ACACATCCAT 
GCCGCACAGA TGTTACTCCG CCGTCAGTAT CCAGGGCTGG GAGGACTACA AGATACGGCA 
GTAGGAGCGT CCGTGTACGG ATATACACGG GTCTCCGGAG ACGGTCTACA GATTCACCAC 
ACCGGATCCC TTCACTGGGT GGTCTCTTCC TCCATAGGTG GACATGTCAG CGTTTATAAC 
AGCATGCCCA GTAAAATAAA TGCGTCACTG GAGTATCAGT TGTGTCAGTG TTACGCCCCA 
CCTCCCAACA GGAACACAGG CGTACTGGCT GTGAAAGTAC CACGTGTACA GCACCAGGGG 
AGCAGGTATA GCTGCGGACT GTTTGCCATA GCCTGGGCTG TAGACATCGC AATGGGGACG 
GATGTGACTC AGGTCAGGTA CAAGGAGAGC AGGATGAGAG CCCATCTCCG GGACTGCTTC 
GAGAGCGGTT ACCTGTCTCC CTTCCCAGGG GCCCGTTGTA TCACCAGTCA GCGACCCAGC 
ACTGAGCACC GGATCACTCT TGCTGCCGGC AAAGGCACCC ATTCAGTTGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Nature453(7198)1064-71(2008 Jun)
Putnam NH,Butts T,Ferrier DE,Furlong RF,Hellsten U,Kawashima T,Robinson-Rechavi M,Shoguchi E,Terry A,Yu JK,Benito-Gutiérrez EL,Dubchak I,Garcia-Fernàndez J,Gibson-Brown JJ,Grigoriev IV,Horton AC,de Jong PJ,Jurka J,Kapitonov VV,Kohara Y,Kuroki Y,Lindquist E,Lucas S,Osoegawa K,Pennacchio LA,Salamov AA,Satou Y,Sauka-Spengler T,Schmutz J,Shin-I T,Toyoda A,Bronner-Fraser M,Fujiyama A,Holland LZ,Holland PW,Satoh N,Rokhsar DS

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BRAFLDRAFT_70615 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

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