piezo1 cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

Gene
Clones
gRNAs

Summary

Gene Symbol	piezo1
Entrez Gene ID	103035442
Full Name	piezo type mechanosensitive ion channel component 1
Gene Type	protein-coding
Organism	Astyanax mexicanus(Mexican tetra)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015606176.1	XP_015461662.1	piezo-type mechanosensitive ion channel component 1
XM_022665129.1	XP_022520850.1	LOW QUALITY PROTEIN: piezo-type mechanosensitive ion channel component 1

Pathways from BioSystems
Gene Ontology

The following piezo1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the piezo1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAs15094	XM_015606176.1 Latest version!	Astyanax mexicanus piezo type mechanosensitive ion channel component 1 (piezo1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OAs99999	XM_022665129.1 Latest version!	Astyanax mexicanus piezo type mechanosensitive ion channel component 1 (piezo1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

piezo1 ( XM_015606176.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_015461662.1 Astyanax mexicanus piezo type mechanosensitive ion channel component 1 (piezo1), mRNA.

Price & Availability↑

CloneID	OAs15094
Clone ID Related Accession (Same CDS sequence)	XM_015606176.1
Accession Version	XM_015606176.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1455033600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	piezo-type mechanosensitive ion channel component 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006738815.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

ATGGAGCTCC AGCTGGGATG CGGATTGCTC TACTGCTTTC TGCTGCCAGC TTTCCTGCTA 
GCAGCCTGCC TGTTCCGCTA CAACGCCCTG TCTTTAGTCT ACCTTCTCTA CCTGCTGCTG 
CTGCCATGGT TCCTCTGGCC CAACAAACAC ACACTGCGAG GACACACAGG CCGGTTCATC 
AAGGCTTTGT TCAGCACCAG TCTGCTCTTT GTGTTGGCTC ACGTCTGCTT CCAGATCTGC 
CTCTACACTG TCCCGGGCCT GGACAATGAG CTGGGACACA ACTGTGAGTG TCTCCAGATC 
ACTGACCCGT CACTGACCCG TCACTTACAC TGTCTCCAGA CTGACTATAA GCTTCCTCTG 
GATGAGCCCT GGACAGTGGT GCGTCTCCTG GCCCCTGATC TGGGTGTCTT CATCATCTCT 
CTGATCACTA TGGTCTTGTG CAACCGCTTG GTGAAGAGTC GAGAGGAAGG ATCCACAGCC 
CAGAACTCCA CAGCCAATCA GGAGGAGTCT GGTACAGATG AGGAGGAAGA GGAGGAGGAT 
GATGAAGATG GGGAGGAAGA TGAAGATGAT GAGGAGGATG GGAGGCAGTC ATCCAGTGCT 
CTTGAGGAAG AGTCCGGCAG AGCCGCTCTG CTGGCCGCCC GTCTGAGAGC CACGGCACAG 
CGCTTCCTCA GGAACATCGG CCGGCTGCTG GCCGTCACCC TGCTGGCACT GGCTGGAATC 
ACATTACCCT CAGCCTTCTC CTCCGTCTAC TTCCTGCTCT TCATTGGAGT GTGTACCTGG 
TGGGCCTGCC ACTTTCCCAT CAGCCACCTG GGCTTCAACG CGCTGTGTGT GATGGTCAGC 
TTCTTCACCG CGGGTCATCT AGTATGTCTG TATCTGTACC AGAGCGACCT CGCCCAGACC 
ATCTTTACCC CAGACAGCCT GTGGGCCAGA CTCTTCGGCC TCAAGGATAT CATCATCCCT 
GCCAACTGCT CCTCCTCCGA TGCCCTCATC CTCAACACAG AACACGATTG GCCCGTCTAC 
GTCAACCCCG GAATCCTGCT CCTCCTCTAC ATCACAGTGG CAACCATCCT GAAGATCAGC 
ACCCACAGTT ACTCAGAGGT GTGGAGTATC ACCTACCACA GCTGGCTGAC CTTCGTGCTG 
CTGTTGTGGG CGTGTCTGAT CTGGATGCTG CGAGCACGCC GACACTTTGC CATGCTGTGC 
TCTCCGTTCA TCCTGCTGTA CGGCCTGGGG CTCTGCTGCC TACAGTACGT CTGGGCCATG 
CACCTGGAGC CCGAGCTGCC CCAGCACGTG GGCTACATGA GCCTGAGGCA GATGGGACTG 
GACCGAGCGA CGTACCCCTG CCTGCGCCTC GGAGCCATGC TGCTTTTCAC TCTGACGTTT 
TGGCTGCTTT TAAGACAATC AGTAAAGGAT ATTTTCTGGA GGAAGAAGAA ACAGACGGCT 
CCACTGCAGG AGGTCACCAC TGGAGAGAAC ACTGGGCGAG ACGACTCGAT TCTGAAGCTG 
CTGGGCCGAG TGGTGATGAG TCTGTACGCT AAGTACTGGA TCTACGTGTG CGGGGGCATG 
TTCATCATGG TCAGCTTCGC CGGGAAACTC GTCGGCTACA AGATTGTCTA CATGTTGCTG 
TTTCTGCTCT GCATGTGTCT CTACCAGGTG TATTACTCCC TGTGGAGGAG ACTGCTGAAA 
CCCTTCTGGT GGCTGGTGGT GGCGTACACC ATGCTAGTGC TCATCGCTAT CTACACCTAC 
CAGTTTGAGG ATTTCCCAGA CTACTGGAGA AACTTCACCG GCTTCACAGA GGAACAGCTG 
TCAGATATTG GTCTGGAGAC GTTTAAGCTG TCGGAGCTTT TCACCAGCAT CATGATCCCC 
GGCTTCTTCC TGCTGGCCTG CATCCTGCAG CTGCACTACT TCCACAAGCC CTTCATGAAG 
ATCACCGACC TGGAACACGT CACACCCCTG CACAGGAGGA GAACAGCCCC GAAGCCGGAG 
GTGCTGCAGA GTTCTGAGGG AGGGGGGTTT GAGGAGGAGG ATCTGCAGAC GGATCTGGGA 
GATGATGAAT CAGAGGATGG TGTGATGGTG CCCAGTAAGT GGGGGCTGGT GATGGACAGG 
CTGATGGTGC TCTTCAGGAA GTTCTCGGAT ATCCTGACGC TGATCCAGGC TTTCCTCTGG 
AGGATCCTGG AGCTCCACAT CCTCAAAATC GTGGCCTTCT TCGTGGTGTG GGTGGCGCTG 
CTGGAGCCGT CTGTGATGAA TCTGGTGTTG GTGGTGTTGT GGTCCTTTGC GATGCCGTAC 
GCTCGATTCA GACCGATGGC CTCCTGTCTG TCCACCGTCT GGGTCTGCGT CATCATCGTC 
TGCAAAATGC TCTACCAGCT CAGCATCGTC AACCCGAACG AGTACTCCAA TAACTGCACT 
GTGCCTCTTT CTAATGAAAC CAGTCTAGAG ATGAATGAGA TTGTGAACTC CACGCTTTAT 
AAAGACCCGG TGGATCCAGC AAACTGGTTT GGGTTCAGGA AGGCTGCCAC TGCTCTGGGA 
TACAGTAAGA ATCACCTGCT GGTTCTGCTG TTGCTGGTGT TCGAGGCGAC GGTGTACCGG 
CACCAGGTTC ATCACTACAG GCAGCTCCAG CGCTCTCCAC CCACCATCCC CGCCATCTTC 
CCCCAGGCCA CACGAGACAA ACTGGACCAG GGTCTGCTGC CCTGCATCAA ATACCTGCTC 
AACTACAGCT TCTACAAGTT CGGCCTGGAG GTCCTTCAGA ATCCTTTTAT ATATTTATAT 
ATCATACAAA TACATAAATT GGACATGATG AAGGTTCTGG TGTTCCGCTA CATGTTCTGG 
TTTGTGTTGG CGGTGGTGTT TGTCACGGGT TCTACTCGGA TCAGTGTGTT TGGGTTGGGG 
TATCTGCTGG CCTCTTTCTT CTTCCTGCTG TTCGGAACCC GACTGCTCAT CAAACGCTCC 
TGCACCCGCC TGCTGATGTG GGACTGTCTC ATCATCTACA ACGTCACTGT CATCATCTCC 
AAGAACATCC TGTCTATTCT GGCGTGTGTG TTTGTAGCTG ATATGCAGGC TCGTTTCTGC 
TGGGTGATTC AGCTCTTCAG TCTGGTCTGC ACAGTGAAAG ACTACTACAA TCCTGCGTCT 
CTGAATGATA AGGAGTGCAG TCTGCCGGTA GAGGAAGCTG GTATTATCTG GGACAGTATC 
TGCTTCTTCT TCCTCCTGCT GCAGAGGAGA GTCTTCCTCA GCTTCTACTT CCTGCACGTG 
ACCGCGGACC TGCAGGCTTC AGCACGCCAG GCCTCCAGGG GTTTCGAGCT GTTCAGAGCC 
AGCATCATAA AGAACATGCG TTTCCACAAG CAGGCAGAGA AGAAATCCAT CCAGCAGCTG 
AAGAACTCGA TGCAGCGAAT CCGTGATAAA CAGCAGAAGT ATAAAGAAGG TCGGAAACCA 
TCTGAAAGAG AAGAACAGCC GACAGAGTCT CAGGAGGAGC TGGAGAGGAA ACAGAGGAAG 
GAGAGGAAGA CCTCCAAACA CAAAGAGTGG CACCGACCCT GGATAGACCA TGCTACTGTT 
CTTCATTCTG GAGATTATTA CCTGTTTGAG TCGGACAGCG GCGAGGAGGA GGACTTCTCA 
ATGGGAGAGC AGAAACAACC CAAACAGACT GCCTTTCAGC TGGCGTATCA GGCGTGGGTG 
AGCAGTGCTA AACATGCTCT CCGTGAACGA CAGAGGAGAC AGAGAGAGCA GAGACTGGAG 
AGAGAGAGAC AACAAGCCGG ATCACAGGCC TCAGGAGGTG ATGGAGAGGA GGCGGGGGCT 
GTTTTTGAGG AGCACGGGTC TCAAATAGAA GAGGAGGAGC TGGGAGAGGA AGGGGAGGAG 
TCAGGTACCC GCAGCGATAC GGTGCAGAGA ATCCTGGACA TCCTAAAGTT CCTCTGGGTT 
CTGTTTCTGG CCATGGTGGA CGGGTTCACG CTGTGGCTCA GCATGCTGAC CAAGCAGTAC 
GTCGACACGT CCACGGTTCT GTCTGAGGAG CGCTACATGC TCATCCATAA CATTGACCAG 
ACGTCCACCA GAGAGCCCAT GGATGACCAG CTCTCTCAGG GCAGTGAGTC CCCCACTCTG 
GAGACGTGTT TAGACGAGAC TGATACTGAT GCTAATATCA GCGGTGCTGT CAGTTCTGCT 
GAGCTGGAGG AAATCAGTGA GTTGGATGGG GCACAGGGCC ACAGCAGCAC CACTCTGAGC 
TCCCATCCCA CCCTGCAGCT GGAGCCTGAC TCACGGACCC TCCGGCGCCA GCGCCGCTCC 
AGAACCGCCA GCGAACTGCT CTCAGACAAG TACCAACTTT TTCAGACTTC TCACCTAAAC 
CTACATGATA GAACCTTAAA ACCTATGTAT GGTCTGCACA GATCTGATTG GCGGAGGAGA 
GCATTTGGTG ATCTTACACC ACACGTGATT GGTCTGTGCA TTTACTGCGC GAAACATATT 
CTGAAACACT CTGCGGCGGC TGATATCGCC TCCACGCTGT CGGAGGATCA GGTGCCCGAG 
GCCTTCCTGG TGATGCTGCT GATCCAGTTC AGCACCATGG TGATCGACCG CGCCCTCTAC 
CTGCGCAAAG CCGTGCTCGG AAAACTCATC TTCCAGATCA TCCTGGTGTT CGGAATCCAT 
CTGTGGATGT TCTTCATCCT ACCTGCAGTC ACTGAGAGGA TGTTTAATCA GAACTTCGTG 
GCTCAGCTCT GGTACTTCGT GAAGTGTATC TACTTCACCC TGTCAGCGTA TCAGGTCCGC 
TGCGGATATC CCACACGCAT CCTCGGAAAC TTCCTCACCA AGAAATACAA CCACCTCAAC 
CTCTTCCTTT TCCAGGGGTT CCGCTTGGTG CCGTTCCTGG TGGAGCTTCG GGCCGTGATG 
GACTGGGTCT GGACCGATAC CACTCTGTCC CTCTCTAACT GGATGTGTGT GGAGGACATT 
TATGCCAACA TCTTCATCAT AAAGTGCAGC CGAGAGACGG AGAAGAAATA CCCTCAGCCT 
AAAGGCCAGA AGAAGAAGAA GATAGTGAAG TACGGAATGG GTGGTCTCAT CGTCTTTTTC 
CTCATCTGCA TCATCTGGTT CCCCCTGCTC TTCATCTCAT TGGTCCGATC AGTTGTGGGT 
GTGGTCAACC ATCCGGTGGA TGTCACTGTA ACAGTCAAGC TGGGAGGTTA TGAGCCCTTG 
TTTACGATGA GTGTGCAGCA GCAGTCCATC CAGCCGTTCA CTGAAGCCAA CTACAACCAT 
CTCACCGAGC TGTTCGGCAG CAATGCGGTG GCGATGCAGT TCATTACACT GTACAGTTAT 
GAGGACATTG TAACGGCCAA CATTGAGGGC AGCTCGGGGT CAGTCTGGAG GATCAGCCCA 
CCCAGCAGAC AGGAGGTCAT AAAGGAGCTG CTGGGCAGCC CTGCGGATAT GACCCTGCGG 
CTGGCCTGGA ACTTCCAGAG GGACCTGAAT AAAGGCGGAA CGGTGGAGAA CACTTTTGAT 
AAGCACTCTA TTAATTTAGA GCCTGGAAAC CCAGTGAGAG CAGACCTGGC CTCTCTGCTG 
GTGGGGAACC GCACTGAGCC AGTTCGAGTG CCGCACATGT TCCCCAACTA CATCAGAGCC 
CCCAACGGAG CCGAGGCCAA GCCGGTCAGC CAGCTGTACG AGGGAGATGA AGAGGGCTAT 
CAGAACGTGA CGCTGTCTCT GATGAGGGAC GGCTCTCTGA ACGGCAGTGG AGCTCAGGAG 
TGGTGGGACA TCAGTGTTGC GGACTGTGAT CAGTCTTCAG GGGCCTGTGG GGTCTTACCC 
ATGATCATCT TCAACGACAA AGTGAGCCCA CCCAGCCTGG GCTTCCTAGC GGGATATGGG 
ATCATGGGGC TGTACGTATC CGTGGTTCTG GTGATCGGTA AGTTCGTGCG AGGCTTCTTC 
AGTGAGATTT CTCACTCCAT CATGTTCGAG GAGCTTCCCT GCGTAGACCG CATCCTCAAA 
CTCTGCACGG ACATCTTCCT GGTGCGGGAG ACGGGCGAGC TGGAGCTGGA GGAGGAGCTT 
TACTCCAAAC TCATCTTCCT CTACCGATCT CCAGAGACCA TGATCAAATG GACCCGGGAG 
AAGAACAAAG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015461662.1
CDS	590..6664
Translation	MELQLGCGLLYCFLLPAFLLAACLFRYNALSLVYLLYLLLLPWFLWPNKHTLRGHTGRFIKALFSTSLLFVLAHVCFQICLYTVPGLDNELGHNCECLQITDPSLTRHLHCLQTDYKLPLDEPWTVVRLLAPDLGVFIISLITMVLCNRLVKSREEGSTAQNSTANQEESGTDEEEEEEDDEDGEEDEDDEEDGRQSSSALEEESGRAALLAARLRATAQRFLRNIGRLLAVTLLALAGITLPSAFSSVYFLLFIGVCTWWACHFPISHLGFNALCVMVSFFTAGHLVCLYLYQSDLAQTIFTPDSLWARLFGLKDIIIPANCSSSDALILNTEHDWPVYVNPGILLLLYITVATILKISTHSYSEVWSITYHSWLTFVLLLWACLIWMLRARRHFAMLCSPFILLYGLGLCCLQYVWAMHLEPELPQHVGYMSLRQMGLDRATYPCLRLGAMLLFTLTFWLLLRQSVKDIFWRKKKQTAPLQEVTTGENTGRDDSILKLLGRVVMSLYAKYWIYVCGGMFIMVSFAGKLVGYKIVYMLLFLLCMCLYQVYYSLWRRLLKPFWWLVVAYTMLVLIAIYTYQFEDFPDYWRNFTGFTEEQLSDIGLETFKLSELFTSIMIPGFFLLACILQLHYFHKPFMKITDLEHVTPLHRRRTAPKPEVLQSSEGGGFEEEDLQTDLGDDESEDGVMVPSKWGLVMDRLMVLFRKFSDILTLIQAFLWRILELHILKIVAFFVVWVALLEPSVMNLVLVVLWSFAMPYARFRPMASCLSTVWVCVIIVCKMLYQLSIVNPNEYSNNCTVPLSNETSLEMNEIVNSTLYKDPVDPANWFGFRKAATALGYSKNHLLVLLLLVFEATVYRHQVHHYRQLQRSPPTIPAIFPQATRDKLDQGLLPCIKYLLNYSFYKFGLEVLQNPFIYLYIIQIHKLDMMKVLVFRYMFWFVLAVVFVTGSTRISVFGLGYLLASFFFLLFGTRLLIKRSCTRLLMWDCLIIYNVTVIISKNILSILACVFVADMQARFCWVIQLFSLVCTVKDYYNPASLNDKECSLPVEEAGIIWDSICFFFLLLQRRVFLSFYFLHVTADLQASARQASRGFELFRASIIKNMRFHKQAEKKSIQQLKNSMQRIRDKQQKYKEGRKPSEREEQPTESQEELERKQRKERKTSKHKEWHRPWIDHATVLHSGDYYLFESDSGEEEDFSMGEQKQPKQTAFQLAYQAWVSSAKHALRERQRRQREQRLERERQQAGSQASGGDGEEAGAVFEEHGSQIEEEELGEEGEESGTRSDTVQRILDILKFLWVLFLAMVDGFTLWLSMLTKQYVDTSTVLSEERYMLIHNIDQTSTREPMDDQLSQGSESPTLETCLDETDTDANISGAVSSAELEEISELDGAQGHSSTTLSSHPTLQLEPDSRTLRRQRRSRTASELLSDKYQLFQTSHLNLHDRTLKPMYGLHRSDWRRRAFGDLTPHVIGLCIYCAKHILKHSAAADIASTLSEDQVPEAFLVMLLIQFSTMVIDRALYLRKAVLGKLIFQIILVFGIHLWMFFILPAVTERMFNQNFVAQLWYFVKCIYFTLSAYQVRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSNWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIVFFLICIIWFPLLFISLVRSVVGVVNHPVDVTVTVKLGGYEPLFTMSVQQQSIQPFTEANYNHLTELFGSNAVAMQFITLYSYEDIVTANIEGSSGSVWRISPPSRQEVIKELLGSPADMTLRLAWNFQRDLNKGGTVENTFDKHSINLEPGNPVRADLASLLVGNRTEPVRVPHMFPNYIRAPNGAEAKPVSQLYEGDEEGYQNVTLSLMRDGSLNGSGAQEWWDISVADCDQSSGACGVLPMIIFNDKVSPPSLGFLAGYGIMGLYVSVVLVIGKFVRGFFSEISHSIMFEELPCVDRILKLCTDIFLVRETGELELEEELYSKLIFLYRSPETMIKWTREKNKD

Target ORF information:

RefSeq Version	XM_015606176.1
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus piezo type mechanosensitive ion channel component 1 (piezo1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015606176.1

ORF Insert Sequence:

ATGGAGCTCC AGCTGGGATG CGGATTGCTC TACTGCTTTC TGCTGCCAGC TTTCCTGCTA 
GCAGCCTGCC TGTTCCGCTA CAACGCCCTG TCTTTAGTCT ACCTTCTCTA CCTGCTGCTG 
CTGCCATGGT TCCTCTGGCC CAACAAACAC ACACTGCGAG GACACACAGG CCGGTTCATC 
AAGGCTTTGT TCAGCACCAG TCTGCTCTTT GTGTTGGCTC ACGTCTGCTT CCAGATCTGC 
CTCTACACTG TCCCGGGCCT GGACAATGAG CTGGGACACA ACTGTGAGTG TCTCCAGATC 
ACTGACCCGT CACTGACCCG TCACTTACAC TGTCTCCAGA CTGACTATAA GCTTCCTCTG 
GATGAGCCCT GGACAGTGGT GCGTCTCCTG GCCCCTGATC TGGGTGTCTT CATCATCTCT 
CTGATCACTA TGGTCTTGTG CAACCGCTTG GTGAAGAGTC GAGAGGAAGG ATCCACAGCC 
CAGAACTCCA CAGCCAATCA GGAGGAGTCT GGTACAGATG AGGAGGAAGA GGAGGAGGAT 
GATGAAGATG GGGAGGAAGA TGAAGATGAT GAGGAGGATG GGAGGCAGTC ATCCAGTGCT 
CTTGAGGAAG AGTCCGGCAG AGCCGCTCTG CTGGCCGCCC GTCTGAGAGC CACGGCACAG 
CGCTTCCTCA GGAACATCGG CCGGCTGCTG GCCGTCACCC TGCTGGCACT GGCTGGAATC 
ACATTACCCT CAGCCTTCTC CTCCGTCTAC TTCCTGCTCT TCATTGGAGT GTGTACCTGG 
TGGGCCTGCC ACTTTCCCAT CAGCCACCTG GGCTTCAACG CGCTGTGTGT GATGGTCAGC 
TTCTTCACCG CGGGTCATCT AGTATGTCTG TATCTGTACC AGAGCGACCT CGCCCAGACC 
ATCTTTACCC CAGACAGCCT GTGGGCCAGA CTCTTCGGCC TCAAGGATAT CATCATCCCT 
GCCAACTGCT CCTCCTCCGA TGCCCTCATC CTCAACACAG AACACGATTG GCCCGTCTAC 
GTCAACCCCG GAATCCTGCT CCTCCTCTAC ATCACAGTGG CAACCATCCT GAAGATCAGC 
ACCCACAGTT ACTCAGAGGT GTGGAGTATC ACCTACCACA GCTGGCTGAC CTTCGTGCTG 
CTGTTGTGGG CGTGTCTGAT CTGGATGCTG CGAGCACGCC GACACTTTGC CATGCTGTGC 
TCTCCGTTCA TCCTGCTGTA CGGCCTGGGG CTCTGCTGCC TACAGTACGT CTGGGCCATG 
CACCTGGAGC CCGAGCTGCC CCAGCACGTG GGCTACATGA GCCTGAGGCA GATGGGACTG 
GACCGAGCGA CGTACCCCTG CCTGCGCCTC GGAGCCATGC TGCTTTTCAC TCTGACGTTT 
TGGCTGCTTT TAAGACAATC AGTAAAGGAT ATTTTCTGGA GGAAGAAGAA ACAGACGGCT 
CCACTGCAGG AGGTCACCAC TGGAGAGAAC ACTGGGCGAG ACGACTCGAT TCTGAAGCTG 
CTGGGCCGAG TGGTGATGAG TCTGTACGCT AAGTACTGGA TCTACGTGTG CGGGGGCATG 
TTCATCATGG TCAGCTTCGC CGGGAAACTC GTCGGCTACA AGATTGTCTA CATGTTGCTG 
TTTCTGCTCT GCATGTGTCT CTACCAGGTG TATTACTCCC TGTGGAGGAG ACTGCTGAAA 
CCCTTCTGGT GGCTGGTGGT GGCGTACACC ATGCTAGTGC TCATCGCTAT CTACACCTAC 
CAGTTTGAGG ATTTCCCAGA CTACTGGAGA AACTTCACCG GCTTCACAGA GGAACAGCTG 
TCAGATATTG GTCTGGAGAC GTTTAAGCTG TCGGAGCTTT TCACCAGCAT CATGATCCCC 
GGCTTCTTCC TGCTGGCCTG CATCCTGCAG CTGCACTACT TCCACAAGCC CTTCATGAAG 
ATCACCGACC TGGAACACGT CACACCCCTG CACAGGAGGA GAACAGCCCC GAAGCCGGAG 
GTGCTGCAGA GTTCTGAGGG AGGGGGGTTT GAGGAGGAGG ATCTGCAGAC GGATCTGGGA 
GATGATGAAT CAGAGGATGG TGTGATGGTG CCCAGTAAGT GGGGGCTGGT GATGGACAGG 
CTGATGGTGC TCTTCAGGAA GTTCTCGGAT ATCCTGACGC TGATCCAGGC TTTCCTCTGG 
AGGATCCTGG AGCTCCACAT CCTCAAAATC GTGGCCTTCT TCGTGGTGTG GGTGGCGCTG 
CTGGAGCCGT CTGTGATGAA TCTGGTGTTG GTGGTGTTGT GGTCCTTTGC GATGCCGTAC 
GCTCGATTCA GACCGATGGC CTCCTGTCTG TCCACCGTCT GGGTCTGCGT CATCATCGTC 
TGCAAAATGC TCTACCAGCT CAGCATCGTC AACCCGAACG AGTACTCCAA TAACTGCACT 
GTGCCTCTTT CTAATGAAAC CAGTCTAGAG ATGAATGAGA TTGTGAACTC CACGCTTTAT 
AAAGACCCGG TGGATCCAGC AAACTGGTTT GGGTTCAGGA AGGCTGCCAC TGCTCTGGGA 
TACAGTAAGA ATCACCTGCT GGTTCTGCTG TTGCTGGTGT TCGAGGCGAC GGTGTACCGG 
CACCAGGTTC ATCACTACAG GCAGCTCCAG CGCTCTCCAC CCACCATCCC CGCCATCTTC 
CCCCAGGCCA CACGAGACAA ACTGGACCAG GGTCTGCTGC CCTGCATCAA ATACCTGCTC 
AACTACAGCT TCTACAAGTT CGGCCTGGAG GTCCTTCAGA ATCCTTTTAT ATATTTATAT 
ATCATACAAA TACATAAATT GGACATGATG AAGGTTCTGG TGTTCCGCTA CATGTTCTGG 
TTTGTGTTGG CGGTGGTGTT TGTCACGGGT TCTACTCGGA TCAGTGTGTT TGGGTTGGGG 
TATCTGCTGG CCTCTTTCTT CTTCCTGCTG TTCGGAACCC GACTGCTCAT CAAACGCTCC 
TGCACCCGCC TGCTGATGTG GGACTGTCTC ATCATCTACA ACGTCACTGT CATCATCTCC 
AAGAACATCC TGTCTATTCT GGCGTGTGTG TTTGTAGCTG ATATGCAGGC TCGTTTCTGC 
TGGGTGATTC AGCTCTTCAG TCTGGTCTGC ACAGTGAAAG ACTACTACAA TCCTGCGTCT 
CTGAATGATA AGGAGTGCAG TCTGCCGGTA GAGGAAGCTG GTATTATCTG GGACAGTATC 
TGCTTCTTCT TCCTCCTGCT GCAGAGGAGA GTCTTCCTCA GCTTCTACTT CCTGCACGTG 
ACCGCGGACC TGCAGGCTTC AGCACGCCAG GCCTCCAGGG GTTTCGAGCT GTTCAGAGCC 
AGCATCATAA AGAACATGCG TTTCCACAAG CAGGCAGAGA AGAAATCCAT CCAGCAGCTG 
AAGAACTCGA TGCAGCGAAT CCGTGATAAA CAGCAGAAGT ATAAAGAAGG TCGGAAACCA 
TCTGAAAGAG AAGAACAGCC GACAGAGTCT CAGGAGGAGC TGGAGAGGAA ACAGAGGAAG 
GAGAGGAAGA CCTCCAAACA CAAAGAGTGG CACCGACCCT GGATAGACCA TGCTACTGTT 
CTTCATTCTG GAGATTATTA CCTGTTTGAG TCGGACAGCG GCGAGGAGGA GGACTTCTCA 
ATGGGAGAGC AGAAACAACC CAAACAGACT GCCTTTCAGC TGGCGTATCA GGCGTGGGTG 
AGCAGTGCTA AACATGCTCT CCGTGAACGA CAGAGGAGAC AGAGAGAGCA GAGACTGGAG 
AGAGAGAGAC AACAAGCCGG ATCACAGGCC TCAGGAGGTG ATGGAGAGGA GGCGGGGGCT 
GTTTTTGAGG AGCACGGGTC TCAAATAGAA GAGGAGGAGC TGGGAGAGGA AGGGGAGGAG 
TCAGGTACCC GCAGCGATAC GGTGCAGAGA ATCCTGGACA TCCTAAAGTT CCTCTGGGTT 
CTGTTTCTGG CCATGGTGGA CGGGTTCACG CTGTGGCTCA GCATGCTGAC CAAGCAGTAC 
GTCGACACGT CCACGGTTCT GTCTGAGGAG CGCTACATGC TCATCCATAA CATTGACCAG 
ACGTCCACCA GAGAGCCCAT GGATGACCAG CTCTCTCAGG GCAGTGAGTC CCCCACTCTG 
GAGACGTGTT TAGACGAGAC TGATACTGAT GCTAATATCA GCGGTGCTGT CAGTTCTGCT 
GAGCTGGAGG AAATCAGTGA GTTGGATGGG GCACAGGGCC ACAGCAGCAC CACTCTGAGC 
TCCCATCCCA CCCTGCAGCT GGAGCCTGAC TCACGGACCC TCCGGCGCCA GCGCCGCTCC 
AGAACCGCCA GCGAACTGCT CTCAGACAAG TACCAACTTT TTCAGACTTC TCACCTAAAC 
CTACATGATA GAACCTTAAA ACCTATGTAT GGTCTGCACA GATCTGATTG GCGGAGGAGA 
GCATTTGGTG ATCTTACACC ACACGTGATT GGTCTGTGCA TTTACTGCGC GAAACATATT 
CTGAAACACT CTGCGGCGGC TGATATCGCC TCCACGCTGT CGGAGGATCA GGTGCCCGAG 
GCCTTCCTGG TGATGCTGCT GATCCAGTTC AGCACCATGG TGATCGACCG CGCCCTCTAC 
CTGCGCAAAG CCGTGCTCGG AAAACTCATC TTCCAGATCA TCCTGGTGTT CGGAATCCAT 
CTGTGGATGT TCTTCATCCT ACCTGCAGTC ACTGAGAGGA TGTTTAATCA GAACTTCGTG 
GCTCAGCTCT GGTACTTCGT GAAGTGTATC TACTTCACCC TGTCAGCGTA TCAGGTCCGC 
TGCGGATATC CCACACGCAT CCTCGGAAAC TTCCTCACCA AGAAATACAA CCACCTCAAC 
CTCTTCCTTT TCCAGGGGTT CCGCTTGGTG CCGTTCCTGG TGGAGCTTCG GGCCGTGATG 
GACTGGGTCT GGACCGATAC CACTCTGTCC CTCTCTAACT GGATGTGTGT GGAGGACATT 
TATGCCAACA TCTTCATCAT AAAGTGCAGC CGAGAGACGG AGAAGAAATA CCCTCAGCCT 
AAAGGCCAGA AGAAGAAGAA GATAGTGAAG TACGGAATGG GTGGTCTCAT CGTCTTTTTC 
CTCATCTGCA TCATCTGGTT CCCCCTGCTC TTCATCTCAT TGGTCCGATC AGTTGTGGGT 
GTGGTCAACC ATCCGGTGGA TGTCACTGTA ACAGTCAAGC TGGGAGGTTA TGAGCCCTTG 
TTTACGATGA GTGTGCAGCA GCAGTCCATC CAGCCGTTCA CTGAAGCCAA CTACAACCAT 
CTCACCGAGC TGTTCGGCAG CAATGCGGTG GCGATGCAGT TCATTACACT GTACAGTTAT 
GAGGACATTG TAACGGCCAA CATTGAGGGC AGCTCGGGGT CAGTCTGGAG GATCAGCCCA 
CCCAGCAGAC AGGAGGTCAT AAAGGAGCTG CTGGGCAGCC CTGCGGATAT GACCCTGCGG 
CTGGCCTGGA ACTTCCAGAG GGACCTGAAT AAAGGCGGAA CGGTGGAGAA CACTTTTGAT 
AAGCACTCTA TTAATTTAGA GCCTGGAAAC CCAGTGAGAG CAGACCTGGC CTCTCTGCTG 
GTGGGGAACC GCACTGAGCC AGTTCGAGTG CCGCACATGT TCCCCAACTA CATCAGAGCC 
CCCAACGGAG CCGAGGCCAA GCCGGTCAGC CAGCTGTACG AGGGAGATGA AGAGGGCTAT 
CAGAACGTGA CGCTGTCTCT GATGAGGGAC GGCTCTCTGA ACGGCAGTGG AGCTCAGGAG 
TGGTGGGACA TCAGTGTTGC GGACTGTGAT CAGTCTTCAG GGGCCTGTGG GGTCTTACCC 
ATGATCATCT TCAACGACAA AGTGAGCCCA CCCAGCCTGG GCTTCCTAGC GGGATATGGG 
ATCATGGGGC TGTACGTATC CGTGGTTCTG GTGATCGGTA AGTTCGTGCG AGGCTTCTTC 
AGTGAGATTT CTCACTCCAT CATGTTCGAG GAGCTTCCCT GCGTAGACCG CATCCTCAAA 
CTCTGCACGG ACATCTTCCT GGTGCGGGAG ACGGGCGAGC TGGAGCTGGA GGAGGAGCTT 
TACTCCAAAC TCATCTTCCT CTACCGATCT CCAGAGACCA TGATCAAATG GACCCGGGAG 
AAGAACAAAG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

piezo1 ( XM_022665129.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_022520850.1 Astyanax mexicanus piezo type mechanosensitive ion channel component 1 (piezo1), mRNA.

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CloneID	OAs99999
Clone ID Related Accession (Same CDS sequence)	XM_022665129.1
Accession Version	XM_022665129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	LOW QUALITY PROTEIN: piezo-type mechanosensitive ion channel component 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019172829.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGAGCTCC AGCTGGGATG CGGATTGCTC TACTGCTTTC TGCTGCCAGC TTTCCTGCTA 
GCAGCCTGCC TGTTGCGCTA CAACGCCCTG TCTTTAGTCT ACCTTCTCTA CCTGCTGCTG 
CTGCCATGGT TCCTCTGGCC CAACAAACAC ACACTGCGAG GACACACAGG CCGGTTCATC 
AAGGCTTTGT TCAGCACCAG TCTGCTCTTT GTGTTGGCTC ACGTCTGCTT CCAGATCTGC 
CTCTACACTG TCCCGGGCCT GGACAATGAG CTGGGACACA ACTGTAGCTC ATGGGAGAAT 
TTGGCAAGGC ACATTGGCGT GTCCAGGCTT CCTCTGGATG AGCCCTGGAC AGTGGTGCGT 
CTCCTGGCTC CTGATCTGGG TGTCTTCATC ATCTCTCTGA TCACTATGGT CTTGTGCAAC 
CGCTTGGTGA AGAGTCGAGA GGAAGGACCC ACAGCCCAGA ACTCCACAGC CAATCAGGAG 
GAGTCTGGTA CAGATGTGGA GGAAGAGGAG GAGGATGATG AAGATGGGGA GGAAGATGAA 
GATGATGAGG AGGATGGGAG GCAGTCATCC AGTGCTCTTG AGGAAGAGTC CGGCAGAGCC 
GCTCTGCTGG CCGCCCGTCT GAGAGCCACG GCACAGCGCT TCCTCAGGAA CATCGGCCGG 
CTGCTGGCCG TCACCCTGCT GGCACTGGCT GGAATCACAT TACCCTCAGC CTTCTCCTCC 
GTCTACTTCC TGCTCTTCAT TGGAGTGTGT ACCTGGTGGG CCTGCCACTT TCCCATCAGC 
CACCTGGGCT TCAACGCGCT GTGTGTGATG GTCAGCTTCT TCACCGCGGG TCATCTAGTA 
TGTCTGTATC TGTACCAGAG CGACCTCGCC CAGACCATCT TTACCCCAGA CAGCCTGTGG 
GCCAGACTCT TCGGCCTCAA GGATATCATC ATCCCTGCCA ACTGCTCCTC CTCCGATGCC 
CTCATCCTCA ACACAGAACA CGATTGGCCC GTCTACGTCA ACCCCGGAAT CCTGCTCCTC 
CTCTACATCA CAGTGGCAAC CATCCTGAAG ATCAGCACCC ACAGTTACTC AGAGACGATT 
GTGGGCGAAG CCACCGTGAC AGAAAGTGGC AGTGGAGAGG AGGTGGAGCT AAAGCAGTGG 
GGGGCAGGAC GAAAAAGCCC TGATGATGAC ACTAAGCAAA TGCTGCTGGC CTCGGGGGAG 
AGCCCTGGGC AGGAATCCAA CCCAACTAAC ACTAAAGAAC ACGTCACTAA CGGTTCCGGT 
CAGCATGACC TCGGGCTCAG TACGTCAGCG GTGCAGCAGG AGGAAGAGGA GGACAGTGCT 
CTGCTCTTAG TGGGCAGGAT GATCATGCAG CAGAGCTACA TCTGTGCTCT CATTGCCATG 
ATGGTGTGGA GTATCACCTA CCACAGCTGG CTGACCTTCG TGCTGCTGTT GTGGGCGTGT 
CTGATCTGGA TGCTGCGAGC GCGCCGACAC TTTGCCATGC TGTGCTCTCC GTTCATCCTG 
CTGTACGGCC TGGGGCTCTG CTGCCTACAG TACGTCTGGG CCATGCACCT GGAGCCCGAG 
CTGCCCCAGC ACGTGGGCTA CATGAGCCTG AGGCAGATGG GACTGGACCG AGCGACGTAC 
CCCTGCCTGC GCCTCGGAGC CATGCTGCTT TTCACTCTGA CGTTTTGGCT GCTTTTAAGA 
CAATCAGTAA AGGATATTTT CTGGAGGAAG AAGAAACAGA CGGCTCCACT GCAGGAGGTC 
ACCACTGGAG AGAACACTGG GCGAGACGAC TCGATTCTGA AGCTGCTGGG CCGAGTGGTG 
ATGAGTCTGT ACGCTAAGTA CTGGATCTAC GTGTGCGGGG GCATGTTCAT CATGGTCAGC 
TTCGCCGGGA AACTTGTCGG CTACAAGATT GTCTACATGT TGCTGTTTCT GCTCTGCATG 
TGTCTCTACC AGGTGTATTA CTCCCTGTGG AGGAGACTGC TGAAACCCTT CTGGTGGCTG 
GTGGTGGCGT ACACCATGCT AGTGCTCATC GCTATCTACA CCTACCAGTT TGAGGATTTC 
CCAGACTACT GGAGAAACTT CACCGGCTTC ACAGAGGAAC AGCTGTCAGA TATTGGTCTG 
GAGACGTTTA AGCTGTCGGA GCTTTTCACC AGCATCATGA TCCCCGGCTT CTTCCTGCTG 
GCCTGCATCC TGCAGCTGCA CTACTTCCAC AAGCCCTTCA TGAAGATCAC CGACCTGGAA 
CACGTCACAC CCCTGCACAG GAGGAGAACA GCCCCGAGGC CGGAGGTGCT GCAGAGTTCT 
GAGGGAAGGG GGTTTGAGGA GGAGGATCTG CAGACGGATC TGGGAGATGA TGAATCAGAG 
GATGGTGTGA TGGTGCCCAG TAAGTGGGGG CTGGTGATGG ACAGGCTGAT GGTGCTCTTC 
AGGAAGTTCT CGGATATCCT GACGCTGATC CAGGCTTTCC TCTGGAGGAT CCTGGAGCTC 
CACATCCTCA AAATCGTGGC CTTCTTCGTG GTGTGGGTGG CGCTGCTGGA GCCGTCTGTG 
ATGAATCTGG TGTTGGTGGT GTTGTGGTCC TTTGCGATGC CGTACGCTCG ATTCAGACCG 
ATGGCCTCCT GTCTGTCCAC CGTCTGGGTC TGCGTCATCA TCGTCTGCAA AATGCTCTAC 
CAGCTCAGCA TCGTCAACCC GAACGAGTAC TCCAATAACT GCACTGTGCC TCTTTCTAAT 
GAAACCAGTC TAGAGATGAA TGAGATTGTG AACTCCACGC TTTATAAAGA CCCGGTGGAT 
CCAGCAAACT GGTTTGGGTT CAGGAAGGCT GCCACTGCTC TGGGATACAG TAAGAATCAC 
CTGCTGGTTC TGCTGTTGCT GGTGTTCGAG GCGACGGTGT ACCGGCACCA GGTTCATCAC 
TACAGGCAGC TCCAGCGCTC TCCACCCACC ATCCCCGCCA TCTTCCCCCA GGCCACACGA 
GACAAACTGG ACCAGGGTCT GCTGCCCTGC ATCAAATACC TGCTCAACTA CAGCTTCTAC 
AAGTTCGGCC TGGAGATCTG CTTTTTGATG ACGGTGAATG TGATTGGCCA GCGTATGAAC 
TTCCTGGTCA TTTTCCACGG CTGCTGGTTG GTAGCGATCA TGGTGAGGCG GCGGCGAGTG 
GCCATCGCCA AAATCTGGTC CAAATACTGT CTATTCCTCG TCATCTTCAT GATCTACCAG 
TACATCCTGT GTGTGGGCAT CCCTCCGGCT CTCTGCATTG ATTATCCATG GAGATGGAAA 
ACCTCAGTCA CCATCAACTC AGCTCTGATC AAATGGATCT ACCTGCCCGA CTTCTACACC 
TCCCCCAACT CCAGAAACCT CATCGCGGAC TTCCTGCTGC TGATGTGTGC GTCTCAGCAG 
TGGAAGGTGT TTGAGAACGA GAAGAAGGAG GAGTGGATGG TGTTGGGCGG AGAGAACAAC 
GATAACCCCG ACCCCATGGA CAGACTGCCC TTTAACCCCG CCCCCAACTT CATCAACTGC 
AGGTCGTATT TGGACATGAT GAAGGTTCTG GTGTTCCGCT ACATGTTCTG GTTTGTGTTG 
GCGGTGGTGT TTGTCACGGG TTCTACTCGG ATCAGTGTGT TTGGGTTGGG GTATCTGCTG 
GCCTCTTTCT TCTTCCTGCT GTTCGGAACC CGACTGCTCA TCAAACGCTC CTGCACCCGC 
CTGCTGATGT GGGACTGTCT CATCATCTAC AACGTCACTG TCATCATCTC CAAGAACATC 
CTGTCTATTC TGGCGTGTGT GTTTGTAGCT GATATGCAGG CTCGTTTCTG CTGGGTGATT 
CAGCTCTTCA GTCTGGTCTG CACAGTGAAA GACTACTACG ATCCTGCGTC TCTGAATGAT 
AAGGAGTGCA GTCTGCCGGT AGAGGAAGCT GGTATTATCT GGGACAGTAT CTGCTTCTTC 
TTCCTCCTGC TGCAGAGGAG AGTCTTCCTC AGCTTCTACT TCCTGCACGT GACCGCGGAC 
CTGCAGGCTT CAGCACGCCA GGCCTCCAGG GGTTTCGAGC TGTTCAGAGC CAGCATCATA 
AAGAACATGC GTTTCCACAA GCAGGCAGAG AAGAAATCCA TCCAGCAGCT GAAGAACTCG 
ATGCAGCGAA TCCGTGATAA ACAGCAGAAG TATAAAGAAG GTCGGAAACC ATCTGAAAGA 
GAAGAACAGC CGACAGAGTC TCAGGAGGAG CTGGAGAGGA AACAGAGGAA GGAGAGGAAG 
ACCTCCAAAC ACAAAGAGTG GCACCGACCC TGGATAGACC ATGCTACTGT TCTTCATTCT 
GGAGATTATT ACCTGTTTGA GTCGGACAGC GGCGAGGAGG AGGACTTCTC AATGGGAGAG 
CAGAAACAAC CCAAACAGAC TGCCTTTCAG CTGGCGTATC AGGCGTGGGT GAGCAGTGCT 
AAACATGCTC TCCGTGAACG ACAGAGGAGA CAGAGAGAGC AGAGACTGGA GAGAGAGAGA 
CAACAAGCCG GATCACAGGC CTCAGGAGGT GATGGAGAGG AGGCGGGGGC TGTTTTTGAG 
GAGCACGGGT CTCAAATAGA AGAGGAGGAG CTGGGAGAGG AAGGGGAGGA GTCAGGTACC 
CGCAGCGATA CGGTGCAGAG AATCCTGGAC ATCCTAAAGT TCCTCTGGGT TCTGTTTCTG 
GCCATGGTGG ACGGGTTCAC GCTGTGGCTC AGCATGCTGA CCAAGCAGTA CGTCGACACG 
TCCACGGTTC TGTCTGAGGA GCGCTACATG CTCATCCATA ACATTGACCA GACGTCCACC 
AGAGAGCCCA TGGATGACCA GCTCTCTCAG GGCAGTGAGT CCCCCACTCT GGAGACGTGT 
TTAGACGAGA CTGATACTGA TGCTAATATC AGCGGTGCTG TCAGTTCTGC TGAGCTGGAG 
GAAATCAGTG AGTTGGATGG GGCACAGGGC CACAGCAGCA CCACTCTGAG CTCCCAGCCC 
ACCCTGCAGC TGGAGCCTGA CTCACGGACC CTCCGGCGCC AGCGCCGCTC CAGAACCGCC 
AGCGAACTGC TCTCAGACAA ACCTTACTGT ATAGAGGAGC TCGTCCACAG TCGAGATTTC 
TACTCGTCTC AGAACCGTAT GCTGAAGCTC CTCTTCGCTC TCTATAACGT TCTGGCGTGT 
CACTCTGAGC TGGCCTGCTA CTTCATCATC GTCCTCAACA ACGCGGTGAC GGCCTCCGTC 
ATCTCCCTGG TCCTGCCCAT CCTCATCTTC CTCTGGGCCA TGCTGGCCGT GCCTCGACCC 
TCCAAGAGCT TCTGGATGAC CGCCATCGTC TACACAGAGG TTATGGTTGT GGTGAAGTAC 
TTGTTCCAGT TTGGCTTCTT CCCCTGGAAC AGTGAATACG AGACCCGGCT GAACGAGAAC 
AAGCCTTTCT TCCCCCCGCG GATCCTCGGC CTGGAGAAAA CCGATAACTA CATCCGCTAC 
GACCTGCTGC AGCTGCTGGC GCTGTTCTTC CACCGCTCTC TGCTGCAGCG CTACGGGCTG 
TGGGATCATG AGGGTCCTCT GGAGGAGAAG GTCAGCCCGT CCTCAGATCA GCAGCAGGAG 
AAGAAGCAGG AGGAAGTGGA GGAGACGGAT ACTGGTTTAA CTGAGCTGAA GGAGAAAACC 
ACAGAGCCTG ACCGAGAGTC CCTAACACAC ACTGATACAC ACACTGATAC ACACACCGAT 
ACACACACTG ATACACACAC TGATACACTC CCGCATACAC ACCCGGACCC TGAGCAGAGC 
GCAGATATAA CCCCTGACCC GGACAGTTTA CCCAGGGAGG AGGGCAAAAG GAAGGGAGGT 
CTGCTGCGCT TCCGCAGGAA GAAATCATCC CGCAAAGACT CTGGAGTAAA GGAGGTGAAG 
GAGGTGAAGA AGAAGAAGGT GAAGGGGAAG CGTGGAGAGG AAGCCAGTAA AAAGATAAAG 
GCTTTCGGGC TGAAACTTAA GAGCGCCTTC ATCTCTGGTG TGAGGAGTGT ATACAGGCCG 
ACTCACAGGT TCTTCCATAA CATCCTGCAT GCTGAGTACC GCGCTGCTAC TGATGTTTAC 
GCTCTGATGT TCCTCACTGA TGTGGTGGAT TTCATCATCA TCGTGTTTGG GTTCTGGGCG 
TTTGGAAAAC ACTCTGCGGC GGCTGATATC GCCTCCACGC TGTCGGAGGA TCAGGTGCCC 
GAGGCCTTCC TGGTGATGCT GCTGATCCAG TTCAGCACCA TGGTGATCGA CCGCGCCCTC 
TACCTGCGCA AAGCCGTGCT CGGAAAACTC ATCTTCCAGA TCATCCTGGT GTTCGGAATC 
CATCTGTGGA TGTTCTTCAT CCTACCTGCA GTCACTGAGA GGATGTTTAA TCAGAACTTC 
GTGGCTCAGC TCTGGTACTT CGTGAAGTGT ATCTACTTCA CCCTGTCAGC GTATCAGGTC 
CGCTGCGGAT ATCCCACACG CATCCTCGGA AACTTCCTCA CCAAGAAATA CAACCACCTC 
AACCTCTTCC TTTTCCAGGG CTTCCGCTTG GTGCCGTTCC TGGTGGAGCT TCGGGCCGTG 
ATGGACTGGG TCTGGACCGA TACCACTCTG TCCCTCTCTA ACTGGATGTG TGTGGAGGAC 
ATTTATGCCA ACATCTTCAT CATAAAGTGC AGCCGAGAGA CGGAGAAGAA ATACCCTCAG 
CCTAAAGGCC AGAAGAAGAA GAAGATAGTG AAGTACGGAA TGGGTGGTCT CATCGTCTTT 
TTCCTCATCT GCATCATCTG GTTCCCCCTG CTCTTCATCT CATTGGTCCG ATCAGTTGTG 
GGTGTGGTCA ACCATCCGGT GGATGTCACT GTAACAGTCA AGCTGGGAGG TTATGAGCCC 
TTGTTTACGA TGAGTGTGCA GCAGCAGTCC ATCCAGCCGT TCACTGAAGC CGACTACAAC 
CATCTCACCG AGCTGTTCGG CAGCAATGCG GTGGCGATGC AGTTCATTAC ACTGTACAGT 
TATGAGGACA TTGTAACGGC CAACATTGAG GGCAGCTCGG GGTCAGTCTG GAGGATCAGC 
CCACCCAGCA GACAGGAGGT CATAAAGGAG CTGCTGGGCA GCCCTGCGGA TATGACCCTG 
CGGCTGGCCT GGAACTTCCA GAGGGACCTG AATAAAGGCG GAACGGTGGA GAACACTTTT 
GATAAGCACT CTATTAATTT AGAGCCTGGA AACCCAGTGA GAGCAGACCT GGCCTCTCTG 
CTGGTGGGGA ACCGCACTGA GCCAGTTCGA GTGCCGCACA TGTTCCCCAA CTACATCAGA 
GCCCCCAACG GAGCCGAGGC CAAGCCGGTC AGCCAGCTGT ACGAGGGAGA TGAAGAGGGC 
TATCAGAACG TGACGCTGTC TCTGATGAGG GACGGCTCTC TGAACGGCAG TGGAGCTCAG 
GAGTGGTGGG ACATCAGTGT TGCGGACTGT GATCAGTCTT CAGGGGCCTG TGGGGTCTTA 
CCCATGATCA TCTTCAACGA CAAAGTGAGC CCACCCAGCC TGGGCTTCCT AGCGGGATAT 
GGGATCATGG GGCTGTACGT ATCCGTGGTT CTGGTGATCG GTAAGTTCGT GCGAGGCTTC 
TTCAGTGAGA TTTCTCACTC CATCATGTTC GAGGAGCTTC CCTGCGTAGA CCGCATCCTC 
AAACTCTGCA CGGACATCTT CCTGGTGCGG GAGACGGGCG AGCTGGAGCT GGAGGAGGAG 
CTTTACTCCA AACTCATCTT CCTCTACCGA TCTCCAGAGA CCATGATCAA ATGGACCCGG 
GAGAAGAACA AAGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022520850.1
CDS	654..8351
Translation	MELQLGCGLLYCFLLPAFLLAACLLRYNALSLVYLLYLLLLPWFLWPNKHTLRGHTGRFIKALFSTSLLFVLAHVCFQICLYTVPGLDNELGHNCSSWENLARHIGVSRLPLDEPWTVVRLLAPDLGVFIISLITMVLCNRLVKSREEGPTAQNSTANQEESGTDVEEEEEDDEDGEEDEDDEEDGRQSSSALEEESGRAALLAARLRATAQRFLRNIGRLLAVTLLALAGITLPSAFSSVYFLLFIGVCTWWACHFPISHLGFNALCVMVSFFTAGHLVCLYLYQSDLAQTIFTPDSLWARLFGLKDIIIPANCSSSDALILNTEHDWPVYVNPGILLLLYITVATILKISTHSYSETIVGEATVTESGSGEEVELKQWGAGRKSPDDDTKQMLLASGESPGQESNPTNTKEHVTNGSGQHDLGLSTSAVQQEEEEDSALLLVGRMIMQQSYICALIAMMVWSITYHSWLTFVLLLWACLIWMLRARRHFAMLCSPFILLYGLGLCCLQYVWAMHLEPELPQHVGYMSLRQMGLDRATYPCLRLGAMLLFTLTFWLLLRQSVKDIFWRKKKQTAPLQEVTTGENTGRDDSILKLLGRVVMSLYAKYWIYVCGGMFIMVSFAGKLVGYKIVYMLLFLLCMCLYQVYYSLWRRLLKPFWWLVVAYTMLVLIAIYTYQFEDFPDYWRNFTGFTEEQLSDIGLETFKLSELFTSIMIPGFFLLACILQLHYFHKPFMKITDLEHVTPLHRRRTAPRPEVLQSSEGRGFEEEDLQTDLGDDESEDGVMVPSKWGLVMDRLMVLFRKFSDILTLIQAFLWRILELHILKIVAFFVVWVALLEPSVMNLVLVVLWSFAMPYARFRPMASCLSTVWVCVIIVCKMLYQLSIVNPNEYSNNCTVPLSNETSLEMNEIVNSTLYKDPVDPANWFGFRKAATALGYSKNHLLVLLLLVFEATVYRHQVHHYRQLQRSPPTIPAIFPQATRDKLDQGLLPCIKYLLNYSFYKFGLEICFLMTVNVIGQRMNFLVIFHGCWLVAIMVRRRRVAIAKIWSKYCLFLVIFMIYQYILCVGIPPALCIDYPWRWKTSVTINSALIKWIYLPDFYTSPNSRNLIADFLLLMCASQQWKVFENEKKEEWMVLGGENNDNPDPMDRLPFNPAPNFINCRSYLDMMKVLVFRYMFWFVLAVVFVTGSTRISVFGLGYLLASFFFLLFGTRLLIKRSCTRLLMWDCLIIYNVTVIISKNILSILACVFVADMQARFCWVIQLFSLVCTVKDYYDPASLNDKECSLPVEEAGIIWDSICFFFLLLQRRVFLSFYFLHVTADLQASARQASRGFELFRASIIKNMRFHKQAEKKSIQQLKNSMQRIRDKQQKYKEGRKPSEREEQPTESQEELERKQRKERKTSKHKEWHRPWIDHATVLHSGDYYLFESDSGEEEDFSMGEQKQPKQTAFQLAYQAWVSSAKHALRERQRRQREQRLERERQQAGSQASGGDGEEAGAVFEEHGSQIEEEELGEEGEESGTRSDTVQRILDILKFLWVLFLAMVDGFTLWLSMLTKQYVDTSTVLSEERYMLIHNIDQTSTREPMDDQLSQGSESPTLETCLDETDTDANISGAVSSAELEEISELDGAQGHSSTTLSSQPTLQLEPDSRTLRRQRRSRTASELLSDKPYCIEELVHSRDFYSSQNRMLKLLFALYNVLACHSELACYFIIVLNNAVTASVISLVLPILIFLWAMLAVPRPSKSFWMTAIVYTEVMVVVKYLFQFGFFPWNSEYETRLNENKPFFPPRILGLEKTDNYIRYDLLQLLALFFHRSLLQRYGLWDHEGPLEEKVSPSSDQQQEKKQEEVEETDTGLTELKEKTTEPDRESLTHTDTHTDTHTDTHTDTHTDTLPHTHPDPEQSADITPDPDSLPREEGKRKGGLLRFRRKKSSRKDSGVKEVKEVKKKKVKGKRGEEASKKIKAFGLKLKSAFISGVRSVYRPTHRFFHNILHAEYRAATDVYALMFLTDVVDFIIIVFGFWAFGKHSAAADIASTLSEDQVPEAFLVMLLIQFSTMVIDRALYLRKAVLGKLIFQIILVFGIHLWMFFILPAVTERMFNQNFVAQLWYFVKCIYFTLSAYQVRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSNWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIVFFLICIIWFPLLFISLVRSVVGVVNHPVDVTVTVKLGGYEPLFTMSVQQQSIQPFTEADYNHLTELFGSNAVAMQFITLYSYEDIVTANIEGSSGSVWRISPPSRQEVIKELLGSPADMTLRLAWNFQRDLNKGGTVENTFDKHSINLEPGNPVRADLASLLVGNRTEPVRVPHMFPNYIRAPNGAEAKPVSQLYEGDEEGYQNVTLSLMRDGSLNGSGAQEWWDISVADCDQSSGACGVLPMIIFNDKVSPPSLGFLAGYGIMGLYVSVVLVIGKFVRGFFSEISHSIMFEELPCVDRILKLCTDIFLVRETGELELEEELYSKLIFLYRSPETMIKWTREKNKD

Target ORF information:

RefSeq Version	XM_022665129.1
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus piezo type mechanosensitive ion channel component 1 (piezo1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022665129.1

ORF Insert Sequence:

ATGGAGCTCC AGCTGGGATG CGGATTGCTC TACTGCTTTC TGCTGCCAGC TTTCCTGCTA 
GCAGCCTGCC TGTTGCGCTA CAACGCCCTG TCTTTAGTCT ACCTTCTCTA CCTGCTGCTG 
CTGCCATGGT TCCTCTGGCC CAACAAACAC ACACTGCGAG GACACACAGG CCGGTTCATC 
AAGGCTTTGT TCAGCACCAG TCTGCTCTTT GTGTTGGCTC ACGTCTGCTT CCAGATCTGC 
CTCTACACTG TCCCGGGCCT GGACAATGAG CTGGGACACA ACTGTAGCTC ATGGGAGAAT 
TTGGCAAGGC ACATTGGCGT GTCCAGGCTT CCTCTGGATG AGCCCTGGAC AGTGGTGCGT 
CTCCTGGCTC CTGATCTGGG TGTCTTCATC ATCTCTCTGA TCACTATGGT CTTGTGCAAC 
CGCTTGGTGA AGAGTCGAGA GGAAGGACCC ACAGCCCAGA ACTCCACAGC CAATCAGGAG 
GAGTCTGGTA CAGATGTGGA GGAAGAGGAG GAGGATGATG AAGATGGGGA GGAAGATGAA 
GATGATGAGG AGGATGGGAG GCAGTCATCC AGTGCTCTTG AGGAAGAGTC CGGCAGAGCC 
GCTCTGCTGG CCGCCCGTCT GAGAGCCACG GCACAGCGCT TCCTCAGGAA CATCGGCCGG 
CTGCTGGCCG TCACCCTGCT GGCACTGGCT GGAATCACAT TACCCTCAGC CTTCTCCTCC 
GTCTACTTCC TGCTCTTCAT TGGAGTGTGT ACCTGGTGGG CCTGCCACTT TCCCATCAGC 
CACCTGGGCT TCAACGCGCT GTGTGTGATG GTCAGCTTCT TCACCGCGGG TCATCTAGTA 
TGTCTGTATC TGTACCAGAG CGACCTCGCC CAGACCATCT TTACCCCAGA CAGCCTGTGG 
GCCAGACTCT TCGGCCTCAA GGATATCATC ATCCCTGCCA ACTGCTCCTC CTCCGATGCC 
CTCATCCTCA ACACAGAACA CGATTGGCCC GTCTACGTCA ACCCCGGAAT CCTGCTCCTC 
CTCTACATCA CAGTGGCAAC CATCCTGAAG ATCAGCACCC ACAGTTACTC AGAGACGATT 
GTGGGCGAAG CCACCGTGAC AGAAAGTGGC AGTGGAGAGG AGGTGGAGCT AAAGCAGTGG 
GGGGCAGGAC GAAAAAGCCC TGATGATGAC ACTAAGCAAA TGCTGCTGGC CTCGGGGGAG 
AGCCCTGGGC AGGAATCCAA CCCAACTAAC ACTAAAGAAC ACGTCACTAA CGGTTCCGGT 
CAGCATGACC TCGGGCTCAG TACGTCAGCG GTGCAGCAGG AGGAAGAGGA GGACAGTGCT 
CTGCTCTTAG TGGGCAGGAT GATCATGCAG CAGAGCTACA TCTGTGCTCT CATTGCCATG 
ATGGTGTGGA GTATCACCTA CCACAGCTGG CTGACCTTCG TGCTGCTGTT GTGGGCGTGT 
CTGATCTGGA TGCTGCGAGC GCGCCGACAC TTTGCCATGC TGTGCTCTCC GTTCATCCTG 
CTGTACGGCC TGGGGCTCTG CTGCCTACAG TACGTCTGGG CCATGCACCT GGAGCCCGAG 
CTGCCCCAGC ACGTGGGCTA CATGAGCCTG AGGCAGATGG GACTGGACCG AGCGACGTAC 
CCCTGCCTGC GCCTCGGAGC CATGCTGCTT TTCACTCTGA CGTTTTGGCT GCTTTTAAGA 
CAATCAGTAA AGGATATTTT CTGGAGGAAG AAGAAACAGA CGGCTCCACT GCAGGAGGTC 
ACCACTGGAG AGAACACTGG GCGAGACGAC TCGATTCTGA AGCTGCTGGG CCGAGTGGTG 
ATGAGTCTGT ACGCTAAGTA CTGGATCTAC GTGTGCGGGG GCATGTTCAT CATGGTCAGC 
TTCGCCGGGA AACTTGTCGG CTACAAGATT GTCTACATGT TGCTGTTTCT GCTCTGCATG 
TGTCTCTACC AGGTGTATTA CTCCCTGTGG AGGAGACTGC TGAAACCCTT CTGGTGGCTG 
GTGGTGGCGT ACACCATGCT AGTGCTCATC GCTATCTACA CCTACCAGTT TGAGGATTTC 
CCAGACTACT GGAGAAACTT CACCGGCTTC ACAGAGGAAC AGCTGTCAGA TATTGGTCTG 
GAGACGTTTA AGCTGTCGGA GCTTTTCACC AGCATCATGA TCCCCGGCTT CTTCCTGCTG 
GCCTGCATCC TGCAGCTGCA CTACTTCCAC AAGCCCTTCA TGAAGATCAC CGACCTGGAA 
CACGTCACAC CCCTGCACAG GAGGAGAACA GCCCCGAGGC CGGAGGTGCT GCAGAGTTCT 
GAGGGAAGGG GGTTTGAGGA GGAGGATCTG CAGACGGATC TGGGAGATGA TGAATCAGAG 
GATGGTGTGA TGGTGCCCAG TAAGTGGGGG CTGGTGATGG ACAGGCTGAT GGTGCTCTTC 
AGGAAGTTCT CGGATATCCT GACGCTGATC CAGGCTTTCC TCTGGAGGAT CCTGGAGCTC 
CACATCCTCA AAATCGTGGC CTTCTTCGTG GTGTGGGTGG CGCTGCTGGA GCCGTCTGTG 
ATGAATCTGG TGTTGGTGGT GTTGTGGTCC TTTGCGATGC CGTACGCTCG ATTCAGACCG 
ATGGCCTCCT GTCTGTCCAC CGTCTGGGTC TGCGTCATCA TCGTCTGCAA AATGCTCTAC 
CAGCTCAGCA TCGTCAACCC GAACGAGTAC TCCAATAACT GCACTGTGCC TCTTTCTAAT 
GAAACCAGTC TAGAGATGAA TGAGATTGTG AACTCCACGC TTTATAAAGA CCCGGTGGAT 
CCAGCAAACT GGTTTGGGTT CAGGAAGGCT GCCACTGCTC TGGGATACAG TAAGAATCAC 
CTGCTGGTTC TGCTGTTGCT GGTGTTCGAG GCGACGGTGT ACCGGCACCA GGTTCATCAC 
TACAGGCAGC TCCAGCGCTC TCCACCCACC ATCCCCGCCA TCTTCCCCCA GGCCACACGA 
GACAAACTGG ACCAGGGTCT GCTGCCCTGC ATCAAATACC TGCTCAACTA CAGCTTCTAC 
AAGTTCGGCC TGGAGATCTG CTTTTTGATG ACGGTGAATG TGATTGGCCA GCGTATGAAC 
TTCCTGGTCA TTTTCCACGG CTGCTGGTTG GTAGCGATCA TGGTGAGGCG GCGGCGAGTG 
GCCATCGCCA AAATCTGGTC CAAATACTGT CTATTCCTCG TCATCTTCAT GATCTACCAG 
TACATCCTGT GTGTGGGCAT CCCTCCGGCT CTCTGCATTG ATTATCCATG GAGATGGAAA 
ACCTCAGTCA CCATCAACTC AGCTCTGATC AAATGGATCT ACCTGCCCGA CTTCTACACC 
TCCCCCAACT CCAGAAACCT CATCGCGGAC TTCCTGCTGC TGATGTGTGC GTCTCAGCAG 
TGGAAGGTGT TTGAGAACGA GAAGAAGGAG GAGTGGATGG TGTTGGGCGG AGAGAACAAC 
GATAACCCCG ACCCCATGGA CAGACTGCCC TTTAACCCCG CCCCCAACTT CATCAACTGC 
AGGTCGTATT TGGACATGAT GAAGGTTCTG GTGTTCCGCT ACATGTTCTG GTTTGTGTTG 
GCGGTGGTGT TTGTCACGGG TTCTACTCGG ATCAGTGTGT TTGGGTTGGG GTATCTGCTG 
GCCTCTTTCT TCTTCCTGCT GTTCGGAACC CGACTGCTCA TCAAACGCTC CTGCACCCGC 
CTGCTGATGT GGGACTGTCT CATCATCTAC AACGTCACTG TCATCATCTC CAAGAACATC 
CTGTCTATTC TGGCGTGTGT GTTTGTAGCT GATATGCAGG CTCGTTTCTG CTGGGTGATT 
CAGCTCTTCA GTCTGGTCTG CACAGTGAAA GACTACTACG ATCCTGCGTC TCTGAATGAT 
AAGGAGTGCA GTCTGCCGGT AGAGGAAGCT GGTATTATCT GGGACAGTAT CTGCTTCTTC 
TTCCTCCTGC TGCAGAGGAG AGTCTTCCTC AGCTTCTACT TCCTGCACGT GACCGCGGAC 
CTGCAGGCTT CAGCACGCCA GGCCTCCAGG GGTTTCGAGC TGTTCAGAGC CAGCATCATA 
AAGAACATGC GTTTCCACAA GCAGGCAGAG AAGAAATCCA TCCAGCAGCT GAAGAACTCG 
ATGCAGCGAA TCCGTGATAA ACAGCAGAAG TATAAAGAAG GTCGGAAACC ATCTGAAAGA 
GAAGAACAGC CGACAGAGTC TCAGGAGGAG CTGGAGAGGA AACAGAGGAA GGAGAGGAAG 
ACCTCCAAAC ACAAAGAGTG GCACCGACCC TGGATAGACC ATGCTACTGT TCTTCATTCT 
GGAGATTATT ACCTGTTTGA GTCGGACAGC GGCGAGGAGG AGGACTTCTC AATGGGAGAG 
CAGAAACAAC CCAAACAGAC TGCCTTTCAG CTGGCGTATC AGGCGTGGGT GAGCAGTGCT 
AAACATGCTC TCCGTGAACG ACAGAGGAGA CAGAGAGAGC AGAGACTGGA GAGAGAGAGA 
CAACAAGCCG GATCACAGGC CTCAGGAGGT GATGGAGAGG AGGCGGGGGC TGTTTTTGAG 
GAGCACGGGT CTCAAATAGA AGAGGAGGAG CTGGGAGAGG AAGGGGAGGA GTCAGGTACC 
CGCAGCGATA CGGTGCAGAG AATCCTGGAC ATCCTAAAGT TCCTCTGGGT TCTGTTTCTG 
GCCATGGTGG ACGGGTTCAC GCTGTGGCTC AGCATGCTGA CCAAGCAGTA CGTCGACACG 
TCCACGGTTC TGTCTGAGGA GCGCTACATG CTCATCCATA ACATTGACCA GACGTCCACC 
AGAGAGCCCA TGGATGACCA GCTCTCTCAG GGCAGTGAGT CCCCCACTCT GGAGACGTGT 
TTAGACGAGA CTGATACTGA TGCTAATATC AGCGGTGCTG TCAGTTCTGC TGAGCTGGAG 
GAAATCAGTG AGTTGGATGG GGCACAGGGC CACAGCAGCA CCACTCTGAG CTCCCAGCCC 
ACCCTGCAGC TGGAGCCTGA CTCACGGACC CTCCGGCGCC AGCGCCGCTC CAGAACCGCC 
AGCGAACTGC TCTCAGACAA ACCTTACTGT ATAGAGGAGC TCGTCCACAG TCGAGATTTC 
TACTCGTCTC AGAACCGTAT GCTGAAGCTC CTCTTCGCTC TCTATAACGT TCTGGCGTGT 
CACTCTGAGC TGGCCTGCTA CTTCATCATC GTCCTCAACA ACGCGGTGAC GGCCTCCGTC 
ATCTCCCTGG TCCTGCCCAT CCTCATCTTC CTCTGGGCCA TGCTGGCCGT GCCTCGACCC 
TCCAAGAGCT TCTGGATGAC CGCCATCGTC TACACAGAGG TTATGGTTGT GGTGAAGTAC 
TTGTTCCAGT TTGGCTTCTT CCCCTGGAAC AGTGAATACG AGACCCGGCT GAACGAGAAC 
AAGCCTTTCT TCCCCCCGCG GATCCTCGGC CTGGAGAAAA CCGATAACTA CATCCGCTAC 
GACCTGCTGC AGCTGCTGGC GCTGTTCTTC CACCGCTCTC TGCTGCAGCG CTACGGGCTG 
TGGGATCATG AGGGTCCTCT GGAGGAGAAG GTCAGCCCGT CCTCAGATCA GCAGCAGGAG 
AAGAAGCAGG AGGAAGTGGA GGAGACGGAT ACTGGTTTAA CTGAGCTGAA GGAGAAAACC 
ACAGAGCCTG ACCGAGAGTC CCTAACACAC ACTGATACAC ACACTGATAC ACACACCGAT 
ACACACACTG ATACACACAC TGATACACTC CCGCATACAC ACCCGGACCC TGAGCAGAGC 
GCAGATATAA CCCCTGACCC GGACAGTTTA CCCAGGGAGG AGGGCAAAAG GAAGGGAGGT 
CTGCTGCGCT TCCGCAGGAA GAAATCATCC CGCAAAGACT CTGGAGTAAA GGAGGTGAAG 
GAGGTGAAGA AGAAGAAGGT GAAGGGGAAG CGTGGAGAGG AAGCCAGTAA AAAGATAAAG 
GCTTTCGGGC TGAAACTTAA GAGCGCCTTC ATCTCTGGTG TGAGGAGTGT ATACAGGCCG 
ACTCACAGGT TCTTCCATAA CATCCTGCAT GCTGAGTACC GCGCTGCTAC TGATGTTTAC 
GCTCTGATGT TCCTCACTGA TGTGGTGGAT TTCATCATCA TCGTGTTTGG GTTCTGGGCG 
TTTGGAAAAC ACTCTGCGGC GGCTGATATC GCCTCCACGC TGTCGGAGGA TCAGGTGCCC 
GAGGCCTTCC TGGTGATGCT GCTGATCCAG TTCAGCACCA TGGTGATCGA CCGCGCCCTC 
TACCTGCGCA AAGCCGTGCT CGGAAAACTC ATCTTCCAGA TCATCCTGGT GTTCGGAATC 
CATCTGTGGA TGTTCTTCAT CCTACCTGCA GTCACTGAGA GGATGTTTAA TCAGAACTTC 
GTGGCTCAGC TCTGGTACTT CGTGAAGTGT ATCTACTTCA CCCTGTCAGC GTATCAGGTC 
CGCTGCGGAT ATCCCACACG CATCCTCGGA AACTTCCTCA CCAAGAAATA CAACCACCTC 
AACCTCTTCC TTTTCCAGGG CTTCCGCTTG GTGCCGTTCC TGGTGGAGCT TCGGGCCGTG 
ATGGACTGGG TCTGGACCGA TACCACTCTG TCCCTCTCTA ACTGGATGTG TGTGGAGGAC 
ATTTATGCCA ACATCTTCAT CATAAAGTGC AGCCGAGAGA CGGAGAAGAA ATACCCTCAG 
CCTAAAGGCC AGAAGAAGAA GAAGATAGTG AAGTACGGAA TGGGTGGTCT CATCGTCTTT 
TTCCTCATCT GCATCATCTG GTTCCCCCTG CTCTTCATCT CATTGGTCCG ATCAGTTGTG 
GGTGTGGTCA ACCATCCGGT GGATGTCACT GTAACAGTCA AGCTGGGAGG TTATGAGCCC 
TTGTTTACGA TGAGTGTGCA GCAGCAGTCC ATCCAGCCGT TCACTGAAGC CGACTACAAC 
CATCTCACCG AGCTGTTCGG CAGCAATGCG GTGGCGATGC AGTTCATTAC ACTGTACAGT 
TATGAGGACA TTGTAACGGC CAACATTGAG GGCAGCTCGG GGTCAGTCTG GAGGATCAGC 
CCACCCAGCA GACAGGAGGT CATAAAGGAG CTGCTGGGCA GCCCTGCGGA TATGACCCTG 
CGGCTGGCCT GGAACTTCCA GAGGGACCTG AATAAAGGCG GAACGGTGGA GAACACTTTT 
GATAAGCACT CTATTAATTT AGAGCCTGGA AACCCAGTGA GAGCAGACCT GGCCTCTCTG 
CTGGTGGGGA ACCGCACTGA GCCAGTTCGA GTGCCGCACA TGTTCCCCAA CTACATCAGA 
GCCCCCAACG GAGCCGAGGC CAAGCCGGTC AGCCAGCTGT ACGAGGGAGA TGAAGAGGGC 
TATCAGAACG TGACGCTGTC TCTGATGAGG GACGGCTCTC TGAACGGCAG TGGAGCTCAG 
GAGTGGTGGG ACATCAGTGT TGCGGACTGT GATCAGTCTT CAGGGGCCTG TGGGGTCTTA 
CCCATGATCA TCTTCAACGA CAAAGTGAGC CCACCCAGCC TGGGCTTCCT AGCGGGATAT 
GGGATCATGG GGCTGTACGT ATCCGTGGTT CTGGTGATCG GTAAGTTCGT GCGAGGCTTC 
TTCAGTGAGA TTTCTCACTC CATCATGTTC GAGGAGCTTC CCTGCGTAGA CCGCATCCTC 
AAACTCTGCA CGGACATCTT CCTGGTGCGG GAGACGGGCG AGCTGGAGCT GGAGGAGGAG 
CTTTACTCCA AACTCATCTT CCTCTACCGA TCTCCAGAGA CCATGATCAA ATGGACCCGG 
GAGAAGAACA AAGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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piezo1 cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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