Fem cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fem
Entrez Gene ID	724970
Full Name	feminizer
Synonyms	GB16868
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001134828.1	NP_001128300.1	feminizer

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Origin of a function by tandem gene duplication limits the evolutionary capability of its sister copy.
Hasselmann M, Lechner S, Schulte C, Beye M
Proceedings of the National Academy of Sciences of the United States of America107(30)13378-83(2010 Jul)

Sex determination in honeybees: two separate mechanisms induce and maintain the female pathway.
Gempe T, Hasselmann M, Schiøtt M, Hause G, Otte M, Beye M
PLoS biology7(10)e1000222(2009 Oct)

Evidence for the evolutionary nascence of a novel sex determination pathway in honeybees.
Hasselmann M, Gempe T, Schiøtt M, Nunes-Silva CG, Otte M, Beye M
Nature454(7203)519-22(2008 Jul)

Evidence for convergent nucleotide evolution and high allelic turnover rates at the complementary sex determiner gene of Western and Asian honeybees.
Hasselmann M, Vekemans X, Pflugfelder J, Koeniger N, Koeniger G, Tingek S, Beye M
Molecular biology and evolution25(4)696-708(2008 Apr)

Insights into social insects from the genome of the honeybee Apis mellifera.

Nature443(7114)931-49(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Fem gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fem cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHd00481	NM_001134828.1 Latest version!	Apis mellifera feminizer (Fem), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fem ( NM_001134828.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> NP_001128300.1 Apis mellifera feminizer (Fem), mRNA.

Price & Availability↑

CloneID	OHd00481
Clone ID Related Accession (Same CDS sequence)	NM_001134828.1
Accession Version	NM_001134828.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556812800000
Organism	Apis mellifera(honey bee)
Product	feminizer
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU101388.1. On Sep 10, 2008 this sequence version replaced XR_014904.1. ##Evidence-Data-START## Transcript exon combination :: EU101388.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00008880, SAMN00113341 [ECO:0000350] ##Evidence-Data-END##

ATGAAACGGA ATACAACAAA TCATTCGCAT CATGATGAGA GATTTAGACA ATCACGCAGT 
GAAGATAGCG AAACTGGTCT GCGTTCAAGA ACACAAGAAG AACGATTACG ACGTAGACGC 
GAATGGATGA TACAACAAGA ACGGGAACGA GAACACGAAA GATTGAAGAA AAAAATGATT 
TTAGAATACG AATTACGACG TGCTCGTGAG AAAAAATTAT CGAAAAGAAG TAAAAGTAGA 
TCCCCAGAAA GCCGAGGTAG AAGTAATGCA TCAAACACGT CTAAAACATT TATATTATCC 
GAAAAATTAG AATCTTCAGA TGGTACATCT TTATTTAGAG GACCAGAAGG TACTCAAGTT 
AGTGCAACAG AACTACGAAA AATTAAGGTA GATATTCATA GAGTTTTGCC AGGAAAACCA 
ACAACAACAT CTGATGAACT TAAACGGGAT ATTATCAATC CTGAAGATGT GATGCTCAAA 
AGAAGAACGG GAGAAGGATC TAAGCCTATA TTTGAAAGAG AAGAAATAAA AAATATATTA 
AATAAAACAA ATGAAATTAC AGAACATCGT ACAGTTTTAG CTGTAAATAT TGAAAAATCA 
GAAAACGAAA CTAAAACATG TAAAAAATAT GCTATTTCGT CAAATTCTTT AAGAAGTCGA 
AGTCGTAGTT TTCAACGTAC ATCATCCTGT CATTCCAGAT ATGAAGATTC AAGACATGAA 
GACAGAAATA GTTATAGAAA TGATGGGGAA AGAAGTTGCA GTAGAGATAG AAGTAGAGAA 
TACAAAAAAA AGGATAGACG ATATGACCAA TTGCACAATG TAGAAGAAAA GCATTTACGA 
GAAAGAACAA GTCGTAGACG TTATTCCCGT TCAAGAGAAC GAGAGCAAAA GTCATATAAA 
AATGAAAGAG AATATCGAGA ATATCGAGAA ACATCGAGAG AAAGATCTCG AGATAGAAGA 
GAACGAGGAA GATCTAGAGA ACATAGAATA ATTCCATCTC ATTATATTGA ACAAATTCCT 
GTTCCTGTTT ACTATGGGAA TTTTCCACCA AGACCAATAA TGGTTAGACC TTGGGTTCCA 
ATGCGGGGTC AAGTACCAGG ATCTAGACAT ATTGGTCCAC TAACACCATT TCCACCACGA 
TTTATTCCTC CAGATATGTA TAGACTTCGT CCACCTCCAA ATCCTAGATT TGGACCTATG 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128300.1
CDS	97..1302
Translation	MKRNTTNHSHHDERFRQSRSEDSETGLRSRTQEERLRRRREWMIQQEREREHERLKKKMILEYELRRAREKKLSKRSKSRSPESRGRSNASNTSKTFILSEKLESSDGTSLFRGPEGTQVSATELRKIKVDIHRVLPGKPTTTSDELKRDIINPEDVMLKRRTGEGSKPIFEREEIKNILNKTNEITEHRTVLAVNIEKSENETKTCKKYAISSNSLRSRSRSFQRTSSCHSRYEDSRHEDRNSYRNDGERSCSRDRSREYKKKDRRYDQLHNVEEKHLRERTSRRRYSRSREREQKSYKNEREYREYRETSRERSRDRRERGRSREHRIIPSHYIEQIPVPVYYGNFPPRPIMVRPWVPMRGQVPGSRHIGPLTPFPPRFIPPDMYRLRPPPNPRFGPMY

Target ORF information:

RefSeq Version	NM_001134828.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera feminizer (Fem), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134828.1

ORF Insert Sequence:

ATGAAACGGA ATACAACAAA TCATTCGCAT CATGATGAGA GATTTAGACA ATCACGCAGT 
GAAGATAGCG AAACTGGTCT GCGTTCAAGA ACACAAGAAG AACGATTACG ACGTAGACGC 
GAATGGATGA TACAACAAGA ACGGGAACGA GAACACGAAA GATTGAAGAA AAAAATGATT 
TTAGAATACG AATTACGACG TGCTCGTGAG AAAAAATTAT CGAAAAGAAG TAAAAGTAGA 
TCCCCAGAAA GCCGAGGTAG AAGTAATGCA TCAAACACGT CTAAAACATT TATATTATCC 
GAAAAATTAG AATCTTCAGA TGGTACATCT TTATTTAGAG GACCAGAAGG TACTCAAGTT 
AGTGCAACAG AACTACGAAA AATTAAGGTA GATATTCATA GAGTTTTGCC AGGAAAACCA 
ACAACAACAT CTGATGAACT TAAACGGGAT ATTATCAATC CTGAAGATGT GATGCTCAAA 
AGAAGAACGG GAGAAGGATC TAAGCCTATA TTTGAAAGAG AAGAAATAAA AAATATATTA 
AATAAAACAA ATGAAATTAC AGAACATCGT ACAGTTTTAG CTGTAAATAT TGAAAAATCA 
GAAAACGAAA CTAAAACATG TAAAAAATAT GCTATTTCGT CAAATTCTTT AAGAAGTCGA 
AGTCGTAGTT TTCAACGTAC ATCATCCTGT CATTCCAGAT ATGAAGATTC AAGACATGAA 
GACAGAAATA GTTATAGAAA TGATGGGGAA AGAAGTTGCA GTAGAGATAG AAGTAGAGAA 
TACAAAAAAA AGGATAGACG ATATGACCAA TTGCACAATG TAGAAGAAAA GCATTTACGA 
GAAAGAACAA GTCGTAGACG TTATTCCCGT TCAAGAGAAC GAGAGCAAAA GTCATATAAA 
AATGAAAGAG AATATCGAGA ATATCGAGAA ACATCGAGAG AAAGATCTCG AGATAGAAGA 
GAACGAGGAA GATCTAGAGA ACATAGAATA ATTCCATCTC ATTATATTGA ACAAATTCCT 
GTTCCTGTTT ACTATGGGAA TTTTCCACCA AGACCAATAA TGGTTAGACC TTGGGTTCCA 
ATGCGGGGTC AAGTACCAGG ATCTAGACAT ATTGGTCCAC TAACACCATT TCCACCACGA 
TTTATTCCTC CAGATATGTA TAGACTTCGT CCACCTCCAA ATCCTAGATT TGGACCTATG 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Origin of a function by tandem gene duplication limits the evolutionary capability of its sister copy.
Proceedings of the National Academy of Sciences of the United States of America107(30)13378-83(2010 Jul)
Hasselmann M,Lechner S,Schulte C,Beye M

Sex determination in honeybees: two separate mechanisms induce and maintain the female pathway.
PLoS biology7(10)e1000222(2009 Oct)
Gempe T,Hasselmann M,Schiøtt M,Hause G,Otte M,Beye M

Evidence for the evolutionary nascence of a novel sex determination pathway in honeybees.
Nature454(7203)519-22(2008 Jul)
Hasselmann M,Gempe T,Schiøtt M,Nunes-Silva CG,Otte M,Beye M

Evidence for convergent nucleotide evolution and high allelic turnover rates at the complementary sex determiner gene of Western and Asian honeybees.
Molecular biology and evolution25(4)696-708(2008 Apr)
Hasselmann M,Vekemans X,Pflugfelder J,Koeniger N,Koeniger G,Tingek S,Beye M

Insights into social insects from the genome of the honeybee Apis mellifera.
Nature443(7114)931-49(2006 Oct)

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Fem cDNA ORF clone, Apis mellifera(honey bee)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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