MARCH6 cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	MARCH6
Entrez Gene ID	552378
Full Name	membrane-associated ring finger (C3HC4) 6
Synonyms	GB15058
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001242458.1	NP_001229387.1	E3 ubiquitin-protein ligase MARCH6
NM_001242458.2	NP_001229387.2	E3 ubiquitin-protein ligase MARCH6

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
Elsik CG, Worley KC, Bennett AK, Beye M, Camara F, Childers CP, de Graaf DC, Debyser G, Deng J, Devreese B, Elhaik E, Evans JD, Foster LJ, Graur D, Guigo R, , Hoff KJ, Holder ME, Hudson ME, Hunt GJ, Jiang H, Joshi V, Khetani RS, Kosarev P, Kovar CL, Ma J, Maleszka R, Moritz RF, Munoz-Torres MC, Murphy TD, Muzny DM, Newsham IF, Reese JT, Robertson HM, Robinson GE, Rueppell O, Solovyev V, Stanke M, Stolle E, Tsuruda JM, Vaerenbergh MV, Waterhouse RM, Weaver DB, Whitfield CW, Wu Y, Zdobnov EM, Zhang L, Zhu D, Gibbs RA,
BMC genomics1586(2014 Jan)

Insights into social insects from the genome of the honeybee Apis mellifera.

Nature443(7114)931-49(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MARCH6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MARCH6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHd00214	NM_001242458.1 Latest version!	Apis mellifera membrane-associated ring finger (C3HC4) 6 (MARCH6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHd34025	NM_001242458.2 Latest version!	Apis mellifera membrane-associated ring finger (C3HC4) 6 (MARCH6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MARCH6 ( NM_001242458.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> NP_001229387.1 Apis mellifera membrane-associated ring finger (C3HC4) 6 (MARCH6), mRNA.

Price & Availability↑

CloneID	OHd00214
Clone ID Related Accession (Same CDS sequence)	NM_001242458.1
Accession Version	NM_001242458.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498320000000
Organism	Apis mellifera(honey bee)
Product	E3 ubiquitin-protein ligase MARCH6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HP545212.1, HP574405.1 and AADG06002975.1. On Jun 8, 2011 this sequence version replaced XR_015037.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00008880, SAMN00113341 [ECO:0000350] ##Evidence-Data-END##

ATGACGGAGG ACATGTCAGG AGCAGACATC TGTAGGGTAT GCAGGTCTGA AGGGCTTGCA 
GACAGGCCTC TTTTTCATCC TTGCATTTGC ACAGGCAGTA TTAAATGGAT ACATCAAGAA 
TGTTTAGTAC AATGGATGCG ATATTCTCGT AAAGAATATT GTGAACTTTG TGGATATCGT 
TTTTCTTTTA CACCAATTTA TAGTCCAGAT ATGCCACGTC GTTTACCATT AAGGGATGTT 
ATTGGAGGAT TATTTTCCAA TATTGTAACA GCAGTAAAAT ATTGGTTGCA CTATACTCTG 
GTTGCAATAG CATGGCTAGG AGTTGTTCCA TTAACAGCTT GTCGAACTTA TAGGGCATTG 
TTTAGTGGAC CTCTTGATCT AGTTCGAATA ATGTCATTAC CAATGGATAT GTTGTCTGCT 
GAAAATATAT CATCAGATGT ATTTCACGGA TGCTTTGTAG TGACATGTAC TTTGTTTGCA 
TTTATTGGTC TAGTGTGGTT AAGGGAACAA ATTTTACATG CTGGTGGACC AGATTGGCTC 
GACCGAGATA ATGTACAATT ACCTCCCGTT GATAATCCAC CACCCAATGC AGAAGTACCA 
ATACAACAAT TCCAAGAGCA AAGAGCTCTT CAACAACAAG AAATTCAAGA TAATAATAAT 
GTACCACCCT TTATTGATGA ACCACCAATC TTACCAGAAC ATTCAAATAA CGATAATTTG 
GAAAATGCAG AAGAACATTG GGCAGAGGAG AATAATTTGG CAAATAATCA GAGAATGGAT 
GAAGTTGAAG GAATTGGTAG AGATGTTGAG CAACATGTTG CTCCCAGAAA TGTAGATCAA 
GAAATAGAAC AACTTGTTAG AAATTTAGAA CCACCTGTGG TAATTCCTCG AAATATAGAA 
CCATTAATAC CACCTAGAGA TGCAGAACAA CCTGCAGTGC CTAGAGACGA ATTGGGTGTA 
AATGGTGAAC AAGCAAATGC AAGAGGTGGT GAAGGATGGA GAGATCAGGC TCAAGGTCAA 
GCACAAGGAG AAGACGAAGA AGCAAACTGG AATCCATTGG AATGGGATAG ACCTGCAGAA 
GAATTAACTT GGGAACGTCT TTTAGGCTTA GATGGATCCC TTGTTTTTCT TGAACATGTA 
TTCTGGGTCG TTTCGTTAAA TACGCTGTTT ATTATGGTTT TTGCCTTCTA TCCATATTAT 
GTCGGTAGTT TTATAATAGC ATTGTTAGAT TTACAAGAAT ATGCAGCTGC TTCACATTTT 
GAGGGATTAG TAACTACATT ATGTGGTTAT TGCGTTACTG GAGTAGGATT AGTAGTCCTT 
CATACATTAG CGGCACCTTT AGGTTTTCAA AGATCTCAAC GGCTATTAGG TTTATGTTAT 
GTTATAGTAA AGGTTTCTTT ACTATCAGTC GTTGAGATAG GAGTACTTCC TTTGGTTTGT 
GGTTGGTGGT TAGATATTTG TTCATTAGCA ATGTTTGACG CTACACTTCG AGATAGAGAA 
TCTTCGTTTA AACTAGCTCC TGGTACTTCA ATGTTTCTAC ATTGGTTGGT GGGAATGATT 
TATATATATT ATTTTGCTTC GTTCATTTTG TTACTACGAG AAGTATTACG GCCAGGAGTA 
CTTTGGTTCT TAAGAAATTT AAATGATCCT GATTTTTCTC CTATCCAAGA AATGATACAT 
CTTCCAATCT TAAGACACGT TAGAAGACTT GTAGCATCTG CAGTAATATT TGGAACTGCT 
ATTTTATTAA TGCTATGGTT ACCTGTGAAA CTTTTAAGAT GGGCATGGCC GGGATTTTTA 
CCATACACCG TGACTATTCA AAGCGAAGCT CAGATTAGCG AATTATCTTT GGAACTGTTA 
TTATTGCAAG TCATTCTTCC TGCATTATTG GAGCAATCTC ATACGCGTAC ATGGTTAAAA 
GCTCTAATAA GAGGCTGGTG TCGAGTTGTA GCTTGGATGC TAGATCTTCA ATCATATCTT 
TTAAGAGAAC AAACTGAAGA TCCTGGACCG GCAGTAATCG AAGAACCGCA ACATCCTGAT 
TTAGGAGCGG CTCATCAAGC ACTGTTACAA AGAGGAGGAC CGACAGGTTT TCAACCATAT 
ATCAGGCCAC GCTGGTTTCC CGCTCGTCTA GTTGGTCTTT TATTTTGTGT TTGCGTTTCT 
CTTGTTATCG CTAGTTTAAT TGCCATGATT CTTCCTGTTT GGCTTGGACG CAGAGTAATG 
GCATTGTGGA TGGTCGGAGC TCCGGCTCCG TCTCCACCTG TATTACCACC TACCTTAAGT 
GGATCTGATA ATAACGAAGC TGCAGTTGCT TTATCTGGAC GAGTGCATGA ACTATATACC 
ATAGCTTGTG GAATGTACGT ATGTTGGGCA GCAGCAAGAG GACTAGCTTT AGCATTTTCT 
TGGTTACCTC GTGGTCGAAG GGCTCTTATA GATAGAATAA AACATTGGGC AATTATAGGT 
GTAAAAGCAT CAGTAGCATT TTTCCTACTT ATTGGCCTAA TACCACTTTT ATTTGGTCTT 
CTTCTTGAAC TAGTCGTTGT AGTACCATTA CGTGTACCAT TAGAACAAAA TGCTATTTTA 
TTCATCTGGC AAGATTGGGC CCTCGGTGTG TTATATACGA AAATAGCAAC CGCTATTATA 
ATGATGGGAC CCGATTGGAG AATTCGTCTT GCAATTGAAC GGGCATACAA CGATGGTATT 
AGAGAAATAG ATTTAAAATT CATCGTTACG GAATTAGCAG CACCAGTGAT ATGTTGTTTC 
GGTCTTGCTT TGGCTGTTCC TTATGCCGTA GCTTATGGAA TAGTCCCACT TTTGATCACG 
AATTTACAAA CGCAAATTCT TATCGCTAGA CATTTATATC CTTTCCTTTT ATTAGTACAC 
GTGGTATGTA TTGTTATATA CTTTCAAATT CGACAGTTTA AAAAGCTTTA CGAACATATA 
AAAAATGACA AATATCTTGT GGGACAAAGA CTCGTAAATT ACGAACATCA AAATAAATCT 
CAACCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229387.1
CDS	146..3154
Translation	MTEDMSGADICRVCRSEGLADRPLFHPCICTGSIKWIHQECLVQWMRYSRKEYCELCGYRFSFTPIYSPDMPRRLPLRDVIGGLFSNIVTAVKYWLHYTLVAIAWLGVVPLTACRTYRALFSGPLDLVRIMSLPMDMLSAENISSDVFHGCFVVTCTLFAFIGLVWLREQILHAGGPDWLDRDNVQLPPVDNPPPNAEVPIQQFQEQRALQQQEIQDNNNVPPFIDEPPILPEHSNNDNLENAEEHWAEENNLANNQRMDEVEGIGRDVEQHVAPRNVDQEIEQLVRNLEPPVVIPRNIEPLIPPRDAEQPAVPRDELGVNGEQANARGGEGWRDQAQGQAQGEDEEANWNPLEWDRPAEELTWERLLGLDGSLVFLEHVFWVVSLNTLFIMVFAFYPYYVGSFIIALLDLQEYAAASHFEGLVTTLCGYCVTGVGLVVLHTLAAPLGFQRSQRLLGLCYVIVKVSLLSVVEIGVLPLVCGWWLDICSLAMFDATLRDRESSFKLAPGTSMFLHWLVGMIYIYYFASFILLLREVLRPGVLWFLRNLNDPDFSPIQEMIHLPILRHVRRLVASAVIFGTAILLMLWLPVKLLRWAWPGFLPYTVTIQSEAQISELSLELLLLQVILPALLEQSHTRTWLKALIRGWCRVVAWMLDLQSYLLREQTEDPGPAVIEEPQHPDLGAAHQALLQRGGPTGFQPYIRPRWFPARLVGLLFCVCVSLVIASLIAMILPVWLGRRVMALWMVGAPAPSPPVLPPTLSGSDNNEAAVALSGRVHELYTIACGMYVCWAAARGLALAFSWLPRGRRALIDRIKHWAIIGVKASVAFFLLIGLIPLLFGLLLELVVVVPLRVPLEQNAILFIWQDWALGVLYTKIATAIIMMGPDWRIRLAIERAYNDGIREIDLKFIVTELAAPVICCFGLALAVPYAVAYGIVPLLITNLQTQILIARHLYPFLLLVHVVCIVIYFQIRQFKKLYEHIKNDKYLVGQRLVNYEHQNKSQP

Target ORF information:

RefSeq Version	NM_001242458.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera membrane-associated ring finger (C3HC4) 6 (MARCH6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242458.1

ORF Insert Sequence:

ATGACGGAGG ACATGTCAGG AGCAGACATC TGTAGGGTAT GCAGGTCTGA AGGGCTTGCA 
GACAGGCCTC TTTTTCATCC TTGCATTTGC ACAGGCAGTA TTAAATGGAT ACATCAAGAA 
TGTTTAGTAC AATGGATGCG ATATTCTCGT AAAGAATATT GTGAACTTTG TGGATATCGT 
TTTTCTTTTA CACCAATTTA TAGTCCAGAT ATGCCACGTC GTTTACCATT AAGGGATGTT 
ATTGGAGGAT TATTTTCCAA TATTGTAACA GCAGTAAAAT ATTGGTTGCA CTATACTCTG 
GTTGCAATAG CATGGCTAGG AGTTGTTCCA TTAACAGCTT GTCGAACTTA TAGGGCATTG 
TTTAGTGGAC CTCTTGATCT AGTTCGAATA ATGTCATTAC CAATGGATAT GTTGTCTGCT 
GAAAATATAT CATCAGATGT ATTTCACGGA TGCTTTGTAG TGACATGTAC TTTGTTTGCA 
TTTATTGGTC TAGTGTGGTT AAGGGAACAA ATTTTACATG CTGGTGGACC AGATTGGCTC 
GACCGAGATA ATGTACAATT ACCTCCCGTT GATAATCCAC CACCCAATGC AGAAGTACCA 
ATACAACAAT TCCAAGAGCA AAGAGCTCTT CAACAACAAG AAATTCAAGA TAATAATAAT 
GTACCACCCT TTATTGATGA ACCACCAATC TTACCAGAAC ATTCAAATAA CGATAATTTG 
GAAAATGCAG AAGAACATTG GGCAGAGGAG AATAATTTGG CAAATAATCA GAGAATGGAT 
GAAGTTGAAG GAATTGGTAG AGATGTTGAG CAACATGTTG CTCCCAGAAA TGTAGATCAA 
GAAATAGAAC AACTTGTTAG AAATTTAGAA CCACCTGTGG TAATTCCTCG AAATATAGAA 
CCATTAATAC CACCTAGAGA TGCAGAACAA CCTGCAGTGC CTAGAGACGA ATTGGGTGTA 
AATGGTGAAC AAGCAAATGC AAGAGGTGGT GAAGGATGGA GAGATCAGGC TCAAGGTCAA 
GCACAAGGAG AAGACGAAGA AGCAAACTGG AATCCATTGG AATGGGATAG ACCTGCAGAA 
GAATTAACTT GGGAACGTCT TTTAGGCTTA GATGGATCCC TTGTTTTTCT TGAACATGTA 
TTCTGGGTCG TTTCGTTAAA TACGCTGTTT ATTATGGTTT TTGCCTTCTA TCCATATTAT 
GTCGGTAGTT TTATAATAGC ATTGTTAGAT TTACAAGAAT ATGCAGCTGC TTCACATTTT 
GAGGGATTAG TAACTACATT ATGTGGTTAT TGCGTTACTG GAGTAGGATT AGTAGTCCTT 
CATACATTAG CGGCACCTTT AGGTTTTCAA AGATCTCAAC GGCTATTAGG TTTATGTTAT 
GTTATAGTAA AGGTTTCTTT ACTATCAGTC GTTGAGATAG GAGTACTTCC TTTGGTTTGT 
GGTTGGTGGT TAGATATTTG TTCATTAGCA ATGTTTGACG CTACACTTCG AGATAGAGAA 
TCTTCGTTTA AACTAGCTCC TGGTACTTCA ATGTTTCTAC ATTGGTTGGT GGGAATGATT 
TATATATATT ATTTTGCTTC GTTCATTTTG TTACTACGAG AAGTATTACG GCCAGGAGTA 
CTTTGGTTCT TAAGAAATTT AAATGATCCT GATTTTTCTC CTATCCAAGA AATGATACAT 
CTTCCAATCT TAAGACACGT TAGAAGACTT GTAGCATCTG CAGTAATATT TGGAACTGCT 
ATTTTATTAA TGCTATGGTT ACCTGTGAAA CTTTTAAGAT GGGCATGGCC GGGATTTTTA 
CCATACACCG TGACTATTCA AAGCGAAGCT CAGATTAGCG AATTATCTTT GGAACTGTTA 
TTATTGCAAG TCATTCTTCC TGCATTATTG GAGCAATCTC ATACGCGTAC ATGGTTAAAA 
GCTCTAATAA GAGGCTGGTG TCGAGTTGTA GCTTGGATGC TAGATCTTCA ATCATATCTT 
TTAAGAGAAC AAACTGAAGA TCCTGGACCG GCAGTAATCG AAGAACCGCA ACATCCTGAT 
TTAGGAGCGG CTCATCAAGC ACTGTTACAA AGAGGAGGAC CGACAGGTTT TCAACCATAT 
ATCAGGCCAC GCTGGTTTCC CGCTCGTCTA GTTGGTCTTT TATTTTGTGT TTGCGTTTCT 
CTTGTTATCG CTAGTTTAAT TGCCATGATT CTTCCTGTTT GGCTTGGACG CAGAGTAATG 
GCATTGTGGA TGGTCGGAGC TCCGGCTCCG TCTCCACCTG TATTACCACC TACCTTAAGT 
GGATCTGATA ATAACGAAGC TGCAGTTGCT TTATCTGGAC GAGTGCATGA ACTATATACC 
ATAGCTTGTG GAATGTACGT ATGTTGGGCA GCAGCAAGAG GACTAGCTTT AGCATTTTCT 
TGGTTACCTC GTGGTCGAAG GGCTCTTATA GATAGAATAA AACATTGGGC AATTATAGGT 
GTAAAAGCAT CAGTAGCATT TTTCCTACTT ATTGGCCTAA TACCACTTTT ATTTGGTCTT 
CTTCTTGAAC TAGTCGTTGT AGTACCATTA CGTGTACCAT TAGAACAAAA TGCTATTTTA 
TTCATCTGGC AAGATTGGGC CCTCGGTGTG TTATATACGA AAATAGCAAC CGCTATTATA 
ATGATGGGAC CCGATTGGAG AATTCGTCTT GCAATTGAAC GGGCATACAA CGATGGTATT 
AGAGAAATAG ATTTAAAATT CATCGTTACG GAATTAGCAG CACCAGTGAT ATGTTGTTTC 
GGTCTTGCTT TGGCTGTTCC TTATGCCGTA GCTTATGGAA TAGTCCCACT TTTGATCACG 
AATTTACAAA CGCAAATTCT TATCGCTAGA CATTTATATC CTTTCCTTTT ATTAGTACAC 
GTGGTATGTA TTGTTATATA CTTTCAAATT CGACAGTTTA AAAAGCTTTA CGAACATATA 
AAAAATGACA AATATCTTGT GGGACAAAGA CTCGTAAATT ACGAACATCA AAATAAATCT 
CAACCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MARCH6 ( NM_001242458.2 ) cDNA ORF clone, Apis mellifera(honey bee) -> NP_001229387.2 Apis mellifera membrane-associated ring finger (C3HC4) 6 (MARCH6), mRNA.

Price & Availability↑

CloneID	OHd34025
Clone ID Related Accession (Same CDS sequence)	NM_001242458.2
Accession Version	NM_001242458.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1545148800000
Organism	Apis mellifera(honey bee)
Product	E3 ubiquitin-protein ligase MARCH6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from QIUM02000063.1. On Dec 20, 2018 this sequence version replaced NM_001242458.1. ##Evidence-Data-START## Transcript exon combination :: GAZV01025967.1, GAZV01025978.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00008880, SAMN00113341 [ECO:0000348] ##Evidence-Data-END##

ATGACGGAGG ACATGTCAGG AGCAGACATC TGTAGGGTAT GCAGGTCTGA AGGCCTTGCA 
GACAGGCCTC TTTTTCATCC TTGCATTTGC ACAGGCAGTA TTAAATGGAT ACATCAAGAA 
TGTTTAGTAC AATGGATGCG ATATTCTCGT AAAGAATATT GTGAACTTTG TGGATATCGT 
TTTTCTTTTA CACCAATTTA TAGTCCAGAT ATGCCACGTC GTTTACCATT AAGGGATGTT 
ATTGGAGGAT TATTTTCCAA TATTGTAACA GCAGTAAAAT ATTGGTTGCA CTATACTCTG 
GTTGCAATAG CATGGCTAGG AGTTGTTCCA TTAACAGCTT GTCGAACTTA TAGGGCATTG 
TTTAGTGGAC CTCTTGATCT AGTTCGAATA ATGTCATTAC CAATGGATAT GTTGTCTGCT 
GAAAATATAT CATCAGATGT ATTTCACGGA TGCTTTGTAG TGACGTGTAC TTTGTTTGCA 
TTTATTGGTC TAGTGTGGTT AAGGGAACAA ATTTTACATG CTGGTGGACC AGATTGGCTC 
GACCGAGATA ATGTACAATT ACCTCCCGTT GATAATCCAC CACCCAATGC AGAAATACCA 
ATACAACAAT TCCAAGAGCA AAGAGCTCTT CAACAACAAG AAATTCAAGA TAATAATAAT 
GTACCACCCT TTATTGATGA ACCACCAATC TTACCAGAAC ATTCAAATAA CGATAATTTG 
GAAAATGCAG AAGAACATTG GGCAGAGGAG AATAATTTGG CAAATAATCA GAGAATGGAT 
GAAGTTGAAG GAATTGGTAG AGATGTTGAG CAACATGTTG CTCCCAGAAA TGTAGATCAA 
GAAATAGAAC AACTTGTTAG AAATTTAGAA CCACCTGTGG TAATTCCTCG AAATATAGAA 
CCATTAATAC CACCTAGAGA TGCAGAACAA CCTGCAGTGC CTAGAGACGA ATTGGGTGTA 
AATGGTGAAC AAGCAAATGC AAGAGGTGGT GAAGGATGGA GAGATCAGGC TCAAGGTCAA 
GCACAAGGAG AAGACGAAGA AGCAAACTGG AATCCATTGG AATGGGATAG ACCTGCAGAA 
GAATTAACTT GGGAACGTCT TTTAGGCTTA GATGGATCCC TTGTTTTTCT TGAACATGTA 
TTCTGGGTCG TTTCGTTAAA TACGCTGTTT ATTATGGTTT TTGCCTTCTA TCCATATTAT 
GTCGGTAGTT TTATAATAGC ATTGTTAGAT TTACAAGAAT ATGCAGCTGC TTCACATTTT 
GAGGGATTAG TAACTACATT GTGTGGTTAT TGCGTTACTG GAGTAGGATT AGTAGTCCTT 
CATACATTAG CGGCACCTTT AGGTTTTCAA AGATCTCAAC GGCTATTAGG TTTATGTTAT 
GTTATAGTAA AGGTTTCTTT ACTATCAGTC GTTGAGATAG GAGTACTTCC TTTGGTTTGT 
GGTTGGTGGT TAGATATTTG TTCATTAGCA ATGTTTGACG CTACACTTCG AGATAGAGAA 
TCTTCGTTTA AACTAGCTCC TGGTACTTCA ATGTTTCTAC ATTGGTTGGT GGGAATGATT 
TATATATATT ATTTTGCTTC GTTCATTTTG TTACTACGAG AAGTATTACG GCCAGGAGTA 
CTTTGGTTCT TAAGAAATTT AAATGATCCT GATTTTTCTC CTATCCAAGA AATGATACAT 
CTTCCAATCT TAAGACACGT TAGAAGACTT GTAGCATCTG CAGTAATATT TGGAACTGCT 
ATTTTATTAA TGCTATGGTT ACCTGTGAAA CTTTTAAGAT GGGCATGGCC GGGATTTTTA 
CCATACACCG TGACTATTCA AAGCGAAGCT CAGATTAGCG AATTATCTTT GGAACTGTTA 
TTATTGCAAG TCATTCTTCC TGCATTATTG GAGCAATCTC ATACGCGTAC ATGGTTAAAA 
GCTCTAATAA GAGGCTGGTG TCGAGTTGTA GCTTGGATGC TAGATCTTCA ATCATATCTT 
TTAAGAGAAC AAACTGAAGA TCCTGGACCG GCAGTAATCG AAGAACCGCA ACATCCTGAT 
TTAGGAGCGG CTCATCAAGC ACTGTTACAA AGAGGAGGAC CGACAGGTTT TCAACCATAT 
ATCAGGCCAC GCTGGTTTCC CGCTCGTCTA GTTGGTCTTT TATTTTGTGT TTGCGTTTCT 
CTTGTTATCG CTAGTTTAAT TGCCATGATT CTTCCTGTTT GGCTTGGACG CAGAGTAATG 
GCATTGTGGA TGGTCGGAGC TCCGGCTCCG TCTCCACCTG TATTACCACC TACCTTAAGT 
GGATCTGATA ATAACGAAGC TGCAGTTGCT TTATCTGGAC GAGTGCATGA ACTATATACC 
ATAGCTTGTG GAATGTACGT ATGTTGGGCA GCAGCAAGAG GACTAGCTTT AGCATTTTCT 
TGGTTACCTC GTGGTCGAAG GGCTCTTATA GATAGAATAA AACATTGGGC AATTATAGGT 
GTAAAAGCAT CAGTAGCATT TTTCCTACTT ATTGGCCTAA TACCACTTTT ATTTGGTCTT 
CTTCTTGAAC TAGTCGTTGT AGTACCATTA CGTGTACCAT TAGAACAAAA TGCTATTTTA 
TTCATCTGGC AAGATTGGGC CCTCGGTGTG TTATATACGA AAATAGCAAC CGCTATTATA 
ATGATGGGAC CCGATTGGAG AATTCGTCTT GCAATTGAAC GGGCATACAA CGATGGTATT 
AGAGAAATAG ATTTAAAATT CATCGTTACG GAATTAGCAG CACCAGTGAT ATGTTGTTTC 
GGTCTTGCTT TGGCTGTTCC TTATGCCGTA GCTTATGGAA TAGTCCCACT TTTGATCACG 
AATTTACAAA CGCAAATTCT TATCGCTAGA CATTTATATC CTTTCCTTTT ATTAGTACAC 
GTGGTATGTA TTGTTATATA CTTTCAAATT CGACAGTTTA AAAAGCTTTA CGAACATATA 
AAAAATGACA AATATCTTGT GGGACAAAGA CTCGTAAATT ACGAACATCA AAATAAATCT 
CAACCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229387.2
CDS	146..3154
Translation	MTEDMSGADICRVCRSEGLADRPLFHPCICTGSIKWIHQECLVQWMRYSRKEYCELCGYRFSFTPIYSPDMPRRLPLRDVIGGLFSNIVTAVKYWLHYTLVAIAWLGVVPLTACRTYRALFSGPLDLVRIMSLPMDMLSAENISSDVFHGCFVVTCTLFAFIGLVWLREQILHAGGPDWLDRDNVQLPPVDNPPPNAEIPIQQFQEQRALQQQEIQDNNNVPPFIDEPPILPEHSNNDNLENAEEHWAEENNLANNQRMDEVEGIGRDVEQHVAPRNVDQEIEQLVRNLEPPVVIPRNIEPLIPPRDAEQPAVPRDELGVNGEQANARGGEGWRDQAQGQAQGEDEEANWNPLEWDRPAEELTWERLLGLDGSLVFLEHVFWVVSLNTLFIMVFAFYPYYVGSFIIALLDLQEYAAASHFEGLVTTLCGYCVTGVGLVVLHTLAAPLGFQRSQRLLGLCYVIVKVSLLSVVEIGVLPLVCGWWLDICSLAMFDATLRDRESSFKLAPGTSMFLHWLVGMIYIYYFASFILLLREVLRPGVLWFLRNLNDPDFSPIQEMIHLPILRHVRRLVASAVIFGTAILLMLWLPVKLLRWAWPGFLPYTVTIQSEAQISELSLELLLLQVILPALLEQSHTRTWLKALIRGWCRVVAWMLDLQSYLLREQTEDPGPAVIEEPQHPDLGAAHQALLQRGGPTGFQPYIRPRWFPARLVGLLFCVCVSLVIASLIAMILPVWLGRRVMALWMVGAPAPSPPVLPPTLSGSDNNEAAVALSGRVHELYTIACGMYVCWAAARGLALAFSWLPRGRRALIDRIKHWAIIGVKASVAFFLLIGLIPLLFGLLLELVVVVPLRVPLEQNAILFIWQDWALGVLYTKIATAIIMMGPDWRIRLAIERAYNDGIREIDLKFIVTELAAPVICCFGLALAVPYAVAYGIVPLLITNLQTQILIARHLYPFLLLVHVVCIVIYFQIRQFKKLYEHIKNDKYLVGQRLVNYEHQNKSQP

Target ORF information:

RefSeq Version	NM_001242458.2
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera membrane-associated ring finger (C3HC4) 6 (MARCH6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242458.2

ORF Insert Sequence:

ATGACGGAGG ACATGTCAGG AGCAGACATC TGTAGGGTAT GCAGGTCTGA AGGCCTTGCA 
GACAGGCCTC TTTTTCATCC TTGCATTTGC ACAGGCAGTA TTAAATGGAT ACATCAAGAA 
TGTTTAGTAC AATGGATGCG ATATTCTCGT AAAGAATATT GTGAACTTTG TGGATATCGT 
TTTTCTTTTA CACCAATTTA TAGTCCAGAT ATGCCACGTC GTTTACCATT AAGGGATGTT 
ATTGGAGGAT TATTTTCCAA TATTGTAACA GCAGTAAAAT ATTGGTTGCA CTATACTCTG 
GTTGCAATAG CATGGCTAGG AGTTGTTCCA TTAACAGCTT GTCGAACTTA TAGGGCATTG 
TTTAGTGGAC CTCTTGATCT AGTTCGAATA ATGTCATTAC CAATGGATAT GTTGTCTGCT 
GAAAATATAT CATCAGATGT ATTTCACGGA TGCTTTGTAG TGACGTGTAC TTTGTTTGCA 
TTTATTGGTC TAGTGTGGTT AAGGGAACAA ATTTTACATG CTGGTGGACC AGATTGGCTC 
GACCGAGATA ATGTACAATT ACCTCCCGTT GATAATCCAC CACCCAATGC AGAAATACCA 
ATACAACAAT TCCAAGAGCA AAGAGCTCTT CAACAACAAG AAATTCAAGA TAATAATAAT 
GTACCACCCT TTATTGATGA ACCACCAATC TTACCAGAAC ATTCAAATAA CGATAATTTG 
GAAAATGCAG AAGAACATTG GGCAGAGGAG AATAATTTGG CAAATAATCA GAGAATGGAT 
GAAGTTGAAG GAATTGGTAG AGATGTTGAG CAACATGTTG CTCCCAGAAA TGTAGATCAA 
GAAATAGAAC AACTTGTTAG AAATTTAGAA CCACCTGTGG TAATTCCTCG AAATATAGAA 
CCATTAATAC CACCTAGAGA TGCAGAACAA CCTGCAGTGC CTAGAGACGA ATTGGGTGTA 
AATGGTGAAC AAGCAAATGC AAGAGGTGGT GAAGGATGGA GAGATCAGGC TCAAGGTCAA 
GCACAAGGAG AAGACGAAGA AGCAAACTGG AATCCATTGG AATGGGATAG ACCTGCAGAA 
GAATTAACTT GGGAACGTCT TTTAGGCTTA GATGGATCCC TTGTTTTTCT TGAACATGTA 
TTCTGGGTCG TTTCGTTAAA TACGCTGTTT ATTATGGTTT TTGCCTTCTA TCCATATTAT 
GTCGGTAGTT TTATAATAGC ATTGTTAGAT TTACAAGAAT ATGCAGCTGC TTCACATTTT 
GAGGGATTAG TAACTACATT GTGTGGTTAT TGCGTTACTG GAGTAGGATT AGTAGTCCTT 
CATACATTAG CGGCACCTTT AGGTTTTCAA AGATCTCAAC GGCTATTAGG TTTATGTTAT 
GTTATAGTAA AGGTTTCTTT ACTATCAGTC GTTGAGATAG GAGTACTTCC TTTGGTTTGT 
GGTTGGTGGT TAGATATTTG TTCATTAGCA ATGTTTGACG CTACACTTCG AGATAGAGAA 
TCTTCGTTTA AACTAGCTCC TGGTACTTCA ATGTTTCTAC ATTGGTTGGT GGGAATGATT 
TATATATATT ATTTTGCTTC GTTCATTTTG TTACTACGAG AAGTATTACG GCCAGGAGTA 
CTTTGGTTCT TAAGAAATTT AAATGATCCT GATTTTTCTC CTATCCAAGA AATGATACAT 
CTTCCAATCT TAAGACACGT TAGAAGACTT GTAGCATCTG CAGTAATATT TGGAACTGCT 
ATTTTATTAA TGCTATGGTT ACCTGTGAAA CTTTTAAGAT GGGCATGGCC GGGATTTTTA 
CCATACACCG TGACTATTCA AAGCGAAGCT CAGATTAGCG AATTATCTTT GGAACTGTTA 
TTATTGCAAG TCATTCTTCC TGCATTATTG GAGCAATCTC ATACGCGTAC ATGGTTAAAA 
GCTCTAATAA GAGGCTGGTG TCGAGTTGTA GCTTGGATGC TAGATCTTCA ATCATATCTT 
TTAAGAGAAC AAACTGAAGA TCCTGGACCG GCAGTAATCG AAGAACCGCA ACATCCTGAT 
TTAGGAGCGG CTCATCAAGC ACTGTTACAA AGAGGAGGAC CGACAGGTTT TCAACCATAT 
ATCAGGCCAC GCTGGTTTCC CGCTCGTCTA GTTGGTCTTT TATTTTGTGT TTGCGTTTCT 
CTTGTTATCG CTAGTTTAAT TGCCATGATT CTTCCTGTTT GGCTTGGACG CAGAGTAATG 
GCATTGTGGA TGGTCGGAGC TCCGGCTCCG TCTCCACCTG TATTACCACC TACCTTAAGT 
GGATCTGATA ATAACGAAGC TGCAGTTGCT TTATCTGGAC GAGTGCATGA ACTATATACC 
ATAGCTTGTG GAATGTACGT ATGTTGGGCA GCAGCAAGAG GACTAGCTTT AGCATTTTCT 
TGGTTACCTC GTGGTCGAAG GGCTCTTATA GATAGAATAA AACATTGGGC AATTATAGGT 
GTAAAAGCAT CAGTAGCATT TTTCCTACTT ATTGGCCTAA TACCACTTTT ATTTGGTCTT 
CTTCTTGAAC TAGTCGTTGT AGTACCATTA CGTGTACCAT TAGAACAAAA TGCTATTTTA 
TTCATCTGGC AAGATTGGGC CCTCGGTGTG TTATATACGA AAATAGCAAC CGCTATTATA 
ATGATGGGAC CCGATTGGAG AATTCGTCTT GCAATTGAAC GGGCATACAA CGATGGTATT 
AGAGAAATAG ATTTAAAATT CATCGTTACG GAATTAGCAG CACCAGTGAT ATGTTGTTTC 
GGTCTTGCTT TGGCTGTTCC TTATGCCGTA GCTTATGGAA TAGTCCCACT TTTGATCACG 
AATTTACAAA CGCAAATTCT TATCGCTAGA CATTTATATC CTTTCCTTTT ATTAGTACAC 
GTGGTATGTA TTGTTATATA CTTTCAAATT CGACAGTTTA AAAAGCTTTA CGAACATATA 
AAAAATGACA AATATCTTGT GGGACAAAGA CTCGTAAATT ACGAACATCA AAATAAATCT 
CAACCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
BMC genomics1586(2014 Jan)
Elsik CG,Worley KC,Bennett AK,Beye M,Camara F,Childers CP,de Graaf DC,Debyser G,Deng J,Devreese B,Elhaik E,Evans JD,Foster LJ,Graur D,Guigo R,,Hoff KJ,Holder ME,Hudson ME,Hunt GJ,Jiang H,Joshi V,Khetani RS,Kosarev P,Kovar CL,Ma J,Maleszka R,Moritz RF,Munoz-Torres MC,Murphy TD,Muzny DM,Newsham IF,Reese JT,Robertson HM,Robinson GE,Rueppell O,Solovyev V,Stanke M,Stolle E,Tsuruda JM,Vaerenbergh MV,Waterhouse RM,Weaver DB,Whitfield CW,Wu Y,Zdobnov EM,Zhang L,Zhu D,Gibbs RA,

Insights into social insects from the genome of the honeybee Apis mellifera.
Nature443(7114)931-49(2006 Oct)

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MARCH6 cDNA ORF clone, Apis mellifera(honey bee)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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