Grp cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	Grp
Entrez Gene ID	552281
Full Name	glycine-rich cuticle protein
Synonyms	GB19234
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001142637.1	NP_001136109.1	glycine-rich cuticle protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
Elsik CG, Worley KC, Bennett AK, Beye M, Camara F, Childers CP, de Graaf DC, Debyser G, Deng J, Devreese B, Elhaik E, Evans JD, Foster LJ, Graur D, Guigo R, , Hoff KJ, Holder ME, Hudson ME, Hunt GJ, Jiang H, Joshi V, Khetani RS, Kosarev P, Kovar CL, Ma J, Maleszka R, Moritz RF, Munoz-Torres MC, Murphy TD, Muzny DM, Newsham IF, Reese JT, Robertson HM, Robinson GE, Rueppell O, Solovyev V, Stanke M, Stolle E, Tsuruda JM, Vaerenbergh MV, Waterhouse RM, Weaver DB, Whitfield CW, Wu Y, Zdobnov EM, Zhang L, Zhu D, Gibbs RA,
BMC genomics1586(2014 Jan)

Ecdysteroid-dependent expression of the tweedle and peroxidase genes during adult cuticle formation in the honey bee, Apis mellifera.
Soares MP, Silva-Torres FA, Elias-Neto M, Nunes FM, Simões ZL, Bitondi MM
PloS one6(5)e20513(2011)

Insights into social insects from the genome of the honeybee Apis mellifera.

Nature443(7114)931-49(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Grp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Grp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHd00247	NM_001142637.1 Latest version!	Apis mellifera glycine-rich cuticle protein (Grp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Grp ( NM_001142637.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> NP_001136109.1 Apis mellifera glycine-rich cuticle protein (Grp), mRNA.

Price & Availability↑

CloneID	OHd00247
Clone ID Related Accession (Same CDS sequence)	NM_001142637.1
Accession Version	NM_001142637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1545062400000
Organism	Apis mellifera(honey bee)
Product	glycine-rich cuticle protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from QIUM02000034.1. On Dec 12, 2008 this sequence version replaced XM_624657.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: FJ380949.1, HP477877.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01985568, SAMN01985569 [ECO:0000348] ##Evidence-Data-END##

ATGGGTCGAG ATGCGAGTGG GGCCAGAGCT AGTATAAAAG AGGGTCCGTG GCATCCGTGG 
AGCATCAGTC CTCAAATCCA AGGAACAGCA GCTATCATGA GGGCATTCAT GATCGTGGTG 
CTCGCCGCTT CAGCAATGGC GCGGCCAGAA GCCGGTTACT CTTACTCTCA ACCCAGCTCC 
TCTTATGGAG CACCAGGAGG TGGAACTACC GGAATTGGTG GAGGATTGGG TGGTGGATTA 
GGTGGTGGAC TTGGAGGTGG ACATGGCGGT GGAATCGGCG GTGGATTGGG TAGTGGATTA 
GGCGGTGGAC TTGGCAGTGG ACTCGGTGGT GGATTCGGTG GAGGTTTCGG TGGTGGCTTT 
GGAGGGGGCA TCGGTGGCGG TGGCGGTGGT GGTTTTGGTG GAGGCGTTGG AGGTGGCTTC 
GGAGGTGGTT CTCTGATTCA AAAACACATT TACGTGCATG TGCCACCACC AGAAGCTCCA 
GAAGACAGAC CAGCTAGACC TATTGCACCA CCACCACCAC CTCAGAAACA TTACAAGATT 
ATATTCATCA AGGCGCCTAC ACCACCAACT CCCACTGCTC CTGTCATTCC TGCTCTTCCT 
CAACAAGATG AACAGAAAAC ATTGATTTAC GTGTTGGTTA AAAAGCCAGA AGAAGCGCCA 
GAAATCACTT TGCCCACCAT TGCTCCTACC CAACCGAGCA AACCAGAAGT ATACTTCATC 
AAATATAAGA CTCAGAAGGA AGTTACTTCT GGTGGTGGAG GCGGTGGCGG CGGTGGTGGA 
ATCGGCGGTG GAATCGGTGG TGGAATTGGA GGCGGAATTG GCGGAGGAAT TGGTGGCGGA 
ATCGGCGGAG GAATCGGTGG AGGAATCGGT GGAGGAATCG GCGGAGGAAT CGGTGGTGGA 
TCCGGTGGAG GCATCGGAGG ACTGGACGGT CATGGCGGCA GCGGACCAAG CGGACCCAGC 
ACATCTTACG GGACACCCGG CGCTTCAGGG CCTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136109.1
CDS	1..999
Translation	MGRDASGARASIKEGPWHPWSISPQIQGTAAIMRAFMIVVLAASAMARPEAGYSYSQPSSSYGAPGGGTTGIGGGLGGGLGGGLGGGHGGGIGGGLGSGLGGGLGSGLGGGFGGGFGGGFGGGIGGGGGGGFGGGVGGGFGGGSLIQKHIYVHVPPPEAPEDRPARPIAPPPPPQKHYKIIFIKAPTPPTPTAPVIPALPQQDEQKTLIYVLVKKPEEAPEITLPTIAPTQPSKPEVYFIKYKTQKEVTSGGGGGGGGGGIGGGIGGGIGGGIGGGIGGGIGGGIGGGIGGGIGGGIGGGSGGGIGGLDGHGGSGPSGPSTSYGTPGASGPY

Target ORF information:

RefSeq Version	NM_001142637.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera glycine-rich cuticle protein (Grp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142637.1

ORF Insert Sequence:

ATGGGTCGAG ATGCGAGTGG GGCCAGAGCT AGTATAAAAG AGGGTCCGTG GCATCCGTGG 
AGCATCAGTC CTCAAATCCA AGGAACAGCA GCTATCATGA GGGCATTCAT GATCGTGGTG 
CTCGCCGCTT CAGCAATGGC GCGGCCAGAA GCCGGTTACT CTTACTCTCA ACCCAGCTCC 
TCTTATGGAG CACCAGGAGG TGGAACTACC GGAATTGGTG GAGGATTGGG TGGTGGATTA 
GGTGGTGGAC TTGGAGGTGG ACATGGCGGT GGAATCGGCG GTGGATTGGG TAGTGGATTA 
GGCGGTGGAC TTGGCAGTGG ACTCGGTGGT GGATTCGGTG GAGGTTTCGG TGGTGGCTTT 
GGAGGGGGCA TCGGTGGCGG TGGCGGTGGT GGTTTTGGTG GAGGCGTTGG AGGTGGCTTC 
GGAGGTGGTT CTCTGATTCA AAAACACATT TACGTGCATG TGCCACCACC AGAAGCTCCA 
GAAGACAGAC CAGCTAGACC TATTGCACCA CCACCACCAC CTCAGAAACA TTACAAGATT 
ATATTCATCA AGGCGCCTAC ACCACCAACT CCCACTGCTC CTGTCATTCC TGCTCTTCCT 
CAACAAGATG AACAGAAAAC ATTGATTTAC GTGTTGGTTA AAAAGCCAGA AGAAGCGCCA 
GAAATCACTT TGCCCACCAT TGCTCCTACC CAACCGAGCA AACCAGAAGT ATACTTCATC 
AAATATAAGA CTCAGAAGGA AGTTACTTCT GGTGGTGGAG GCGGTGGCGG CGGTGGTGGA 
ATCGGCGGTG GAATCGGTGG TGGAATTGGA GGCGGAATTG GCGGAGGAAT TGGTGGCGGA 
ATCGGCGGAG GAATCGGTGG AGGAATCGGT GGAGGAATCG GCGGAGGAAT CGGTGGTGGA 
TCCGGTGGAG GCATCGGAGG ACTGGACGGT CATGGCGGCA GCGGACCAAG CGGACCCAGC 
ACATCTTACG GGACACCCGG CGCTTCAGGG CCTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
BMC genomics1586(2014 Jan)
Elsik CG,Worley KC,Bennett AK,Beye M,Camara F,Childers CP,de Graaf DC,Debyser G,Deng J,Devreese B,Elhaik E,Evans JD,Foster LJ,Graur D,Guigo R,,Hoff KJ,Holder ME,Hudson ME,Hunt GJ,Jiang H,Joshi V,Khetani RS,Kosarev P,Kovar CL,Ma J,Maleszka R,Moritz RF,Munoz-Torres MC,Murphy TD,Muzny DM,Newsham IF,Reese JT,Robertson HM,Robinson GE,Rueppell O,Solovyev V,Stanke M,Stolle E,Tsuruda JM,Vaerenbergh MV,Waterhouse RM,Weaver DB,Whitfield CW,Wu Y,Zdobnov EM,Zhang L,Zhu D,Gibbs RA,

Ecdysteroid-dependent expression of the tweedle and peroxidase genes during adult cuticle formation in the honey bee, Apis mellifera.
PloS one6(5)e20513(2011)
Soares MP,Silva-Torres FA,Elias-Neto M,Nunes FM,Simões ZL,Bitondi MM

Insights into social insects from the genome of the honeybee Apis mellifera.
Nature443(7114)931-49(2006 Oct)

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Grp cDNA ORF clone, Apis mellifera(honey bee)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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