LOC411961 cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC411961
Entrez Gene ID	411961
Full Name	E3 ubiquitin-protein ligase HUWE1
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_026442186.1	XP_026297971.1	E3 ubiquitin-protein ligase HUWE1 isoform X1
XM_016913304.1	XP_016768793.1	LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase HUWE1
XM_026442187.1	XP_026297972.1	E3 ubiquitin-protein ligase HUWE1 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC411961 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC411961 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHd22517	XM_016913304.1 Latest version!	Apis mellifera E3 ubiquitin-protein ligase HUWE1 (LOC411961), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHd32885	XM_026442186.1 Latest version!	Apis mellifera E3 ubiquitin-protein ligase HUWE1 (LOC411961), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHd32886	XM_026442187.1 Latest version!	Apis mellifera E3 ubiquitin-protein ligase HUWE1 (LOC411961), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC411961 ( XM_016913304.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_016768793.1 Apis mellifera E3 ubiquitin-protein ligase HUWE1 (LOC411961), mRNA.

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CloneID	OHd22517
Clone ID Related Accession (Same CDS sequence)	XM_016913304.1
Accession Version	XM_016913304.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1463932800000
Organism	Apis mellifera(honey bee)
Product	LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase HUWE1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007077.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis mellifera Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 2 indels internal stop codons :: corrected 2 genomic stop codons ##RefSeq-Attributes-END##

ATGAAAATTG ATCGCAATAG ATTGAGAAAA TATACATCAG AAGCGCCTGC AAAATGTCAG 
GCGCTTATAA ACAAGTTGCG CTCATGCTCC CATGCAGAAT TGCTAGAGGA GCTAAAGCAA 
ATAGATGCAT GGACTTTTGG TAAATGTGAA TTATTTCATT GGGTCGATGT ATTGGACCTT 
AGTGACAGCA TTTTGGAAGA AGCTGCTGCT AAAAGCCCAG ATAATCCATG GGAATTGGCT 
TGTGATCTCC CTCAAAATAG AGAGGCAAAA GAACTTCTCC TTTGGGTTCT ACATCTTACA 
ACATTATTGA TTGAACATTC ATATTCGCGA CATTTATACA GCAGTATGGA ACATCTAGTG 
ACTTTATTAT CAAGCTGTGA CATGCATGTT GTCCTTGGTG TATTAAATAT TCTCTACATG 
TTCAGTAAAC GTAGCAATTT CATTGCACGT TTAAATAGTG ACAAGAGACA AGCTTTATTA 
AGTAGACTTA CTCTTTTAGC AGAGAGTTGG GGAGGTAAAG AAAATGGATT TGGTTTAGCA 
GAATGTTGTA AAGAATATCC AGATAAGCCA CTTCCAGCAA GTGCCACAAC ATTGCATTTC 
GAATTTTATG CAGAAAATTC AACGACAGAT TCAAATGCCA CATCAAACAT TGGCACAAAA 
AAATCTGGAC AGACGAATTT TGTAACATGT ATACATATAG AAAATGTAGA TAAACTGGGT 
AAAACACCAG CACAAATTAT GAATGATTTG TTAAAAGTCA ATAACGTACC CCAAGATCGA 
CAGATTGCGT TATTTACACA TATAAGATTA GCACACAGTT TCTCTGACTA TAGAAGACGA 
TTACAATGTG TGCACGCTCG GCTACAGGCA TTATCAATAC TTGTATATAA TAGTGAACTA 
GAAGAAAATG CACATAGTTT GTTGCATCCT GGACTTTTAG AAGAATTAGT TGAATTACTG 
GAACTTCCAC ATGCACATCT TGTTGAATTA CGTGCTGCTG CTTTAAGAAC TCTTACAAGT 
ATCATTCATT TAGATCGCAA TCCACATTTT CCAAAAAAAC CTAGTTCAAG ATTAAATATG 
ATAATCGATG TCACTGGAGC CAATTCATAT CATGGATTTT TTCCTGTATT AATTCGAACT 
TGCATTTCAA CATTAATTTC TCCACAACCT GAAGGACAAC CATTTCCTTT GTCACTTGCA 
ACAGCTTTAT TTAGCTTTCT TTACCATCTT TCAAGTTATG AAGCAGGATG TGAAGCACTT 
GTTAGTTGTG GGATGATGGA AAGTTTGTTA AAAATCATGA ATTGGCCTGG TAGCGAGTTT 
GAACATTTGA CGTTTGTAAC AAGGGCAGTT CGTGTTATAG ACTTAATAAC AAATATAGAT 
ATGCAAGGAT TTCAAGCTCA TGGTGGTTTA GCCAGTTTTA TTAATCGTCT TGATATGGAA 
GTAAATGTTT GTAGAAAAGA ACAACCATTT GAAATAGAAC CAATACATTA TCAAGATAAT 
TCTCAGGCCA CTCGTGAAAG ATCAATGGAT CGCGATGAAA GTTCTACGTC TTCACGTGCT 
TTTGATGAGC GTGAAGAATC ATCTAATCCT ATGGAATTTT CGGAAGATGA GAACATGAGT 
TCCAAAACCG ATGATAAAAA TGAACAAATA CCAGATTATT CAGCTGGAAA AACTGGAAAA 
ACTTGTCTAC CACAGCGAGT TACACTTTTA AAAGCGTTAT TAAATTTTAT TAAAAAAATT 
ATTCAAGATC CTACATTTTC TGATAGTATT AGACATGTTA TGGAGGGTAC TTTACCTTCT 
TCCTTGAGAC ATATTATCAG TAATTCAGAA TATTATGGTC CTTCTTTGTT CCTTATTGCA 
ACAGATGCTG TAACAGTATA TGTTTTCCAA GAGCCATCTT TATTATCTTC TTTACAGGAT 
AATGGATTAA CTGATCTTGT ATTGCATGCT TTACTAATTA AAGAAATTCC TGCTAGTAGA 
GAAGTTCTAG GATCATTGCC TAATGTATTT AGTGCTTTAT GTTTAAATCA GCGTGGACTA 
GAATCATTTG TAAAACGACG TCCTTTCGAA CGCTTATTTA AAGTATTATT ATCGCCACTT 
TATCTGCCTG CAATGAATCG ACGTCGTAGC ACAGATCCTT TAGGTGATAC TGCTTCGAAT 
TTGGGTAATG CAATGGATGA ATTAATGCGA CATCAGCCAA GTTTGAAGGT TGATGCAATC 
GCTGCTGTTA TAAAGTTATT AGAAGAACTT TGTGTACTGG GTTGTGATCC TCGTTATATA 
TGCTTGCAAG CTGAAGATAA ATTCGATAAT TCTCAATCAA ATACTACTTC TGGAAATCGT 
CAATCTTCTG GACAATCTGT TGGAGTTGGA GTTGGAGATT CTGGTGTAGG AGGAGGAGGT 
GGTAGTGGAA GTAGTAGCGA CGAAGAAGAA GAAGATGAAG AAGAAGCTAG TACAAGTTCT 
CATCCACAAC GTGAAGAACA ATGTTCATCA TCCACTGCTC AACCAGTTCC ACCACCTCAA 
CCTAGAGAAA AAACTCCAAT AGCTTTAATG GAATATATAC AAAATGTTAC AAAATTCGTT 
GATGCTATTC TCAGTAATAA TTCCACGGAT GATCATTGTA GAGAATTTGT CGCCCAAAAA 
GGTCTCGTAC CATTATTGTC AATTTTAGGA TTACCTAATC TTCCAGTGGA TCATTCAGTT 
GCCCAAACGG CACAAGCTGT AGCTACAGTT TGTAAAAGTA TTTTAAATCT CGCACATGAA 
CCATTAGTTC TTAAAACTGG TCTATCTCAG TTGAATGAAG TTTTGAATTT GCTGAAACCA 
TTATACAATT ATATGGAAGC TCCAGGAGGA TCTGTTTTAT TACATGAACT TGTCAATGCT 
CCAAATCTGG AAACAGCTTT TACCAGTGAT ACGGCAACAC CTTTACTTCA TGCTATGAAT 
GCTGCTTATG GATATATAAT AATGTTTGCA CAAGTGTGCC GTACCAGTCA AAGTGAAATC 
AGAAACTTGG CTATGAATCA TTGGGGTTCT GAACTTGGTT TGACTGTTCT GAAAGGTTTA 
TCAGAATTAT ATACAGCTCT AATCTGGGAA AGTACAGCCT TATTAGCACT TACCACGGAA 
GATTTTATTC CGGCTGGTTG TGATTTTGGA AAAGAAGATA TGGATAAACT TATACGTCCT 
GAATCAACAA ATGAAGGTGA AAGTACTAGT TGGTCTGGTG CTACATCTAC AGATATGGGA 
AGTAATGGGA TGACTACAGC TATGGAAGCT TTAAGCACTG ATCCGCCTCC TATACCTATG 
GAAGTAGACG AAAAACCAAG TGTTAGTACA GGAAGAATAT CTCCAAATTC TCATCGACTT 
ACATATCCTA AACATTTGCT GGGACTTACA TCTAAAATGG GCAGAGCTTT AGCAGAACTC 
TTTGCTATAC TTGTAAAGCT TTGCATGGGC TCTCCAATTA GACAACGCAG AGGACAACAG 
ATGCCTCCAA TACCAGCCTT ACCTTCACAA GCATCTAGAA GTGTAGCAAC TGCTTTAAAT 
TGTGTATTAA TTCGCGGATT ATCATGGGAT AAATTACCAC CATCGACAGT GCCAAAACTT 
CAAGTAACCT TTTTGATGTG CACTTTAAGT TTTGTATCTC CTGCATTATT TGATGAAAAG 
GGGTATCCAT TTCATTTGAT GCTTCAAAAG TTCGTTAAAC TCGGCGGACA AAATGCATTT 
TTCGCTGCTT TTCATTGGGT ATTATCTGGA GGAGGACAGT TTCCGTTATC TGAAGGATTA 
GAGCATCCAA ATTTACCTGA TTGTACTGGA GAATTCTTAG ATGGTTGGTT GATGATTTTA 
GAAAAAATGG TGAATCCTAA AACTCTTCTT GATTCTCCAT ATGTTGTTTC TTCAAAATGT 
ACTGGAATAT ATAAAGTATA TGAAACATTT GATCCTATAA AATATCTTAC GGATATTCAT 
AAGGAAGCTT TCGAGGCAGT GATGCTAATG TGGGGAAAGA AACCTTTAAA AAATTATGGA 
GTTCATATGA CAGAACTTAT TTTATCTATC TTAAGACATA TTTTACGAAG TGAGCAGATC 
ATAAAGGAAC ATGCTGAAAA AGAAGAAAAT AATGAAGGAG GTATTAGTGG TAGTACTAGT 
GGAACTAGTG GAATCGGAAG AGGTGGAACA ATATCCGATC GCAGAGAAGA ACCAGAAGCA 
GATGTAAATC CAGAACATCT AAGACAGTTG ATGGATATGG GTTTCAGTAG AGCTCATTGC 
ATAGAAGCTT TGCTTCATAC ATTGACAGTT GAACAAGCTA CTGATTATTT GTTAACTAAT 
CCTGCTACTC TACGTCGATC AGATGTGCCA GCAGGTGATG TAAGCGGACA AGATTTAATA 
ATGGATTTAG AATTGGTAGA TGATGATCAA ATAATGCATG CCATAGCGAT GTCGCTTGGA 
GAAACGGCGG AAACGCGAAA TGTTTTCAGC ACAGAAGAAT CCAGTTCACG TGAGGCTACT 
ACCATAACTG AAAGCATAGA TAAATTTACG CAAAATGCTT TAAGTCAATG TTTAAATTTA 
TTGGATCTCA TGCCTGATAC TGTATACAGA ATTTGCGATT TATTAGTTGC CATCGCTAAA 
AGAAACGGAG ATGAATGGCG TGATAATATG TTGCGACAAC TTATGAAAGA AATTGGAGGT 
TTGGTAGATT ATTTAATCGA AGTTGCTGAA AGTCAAGACC CGAATGCTGG AGTAAAATTA 
GTAGAATGTA AAGAAGCTAA TAAAGTTACT GGACGTATTT ATCTGTATAC AATGTTCTTT 
GAAGGAACAT TCCAAGAAAT GCGAGTGCCC TGTGCTCAAA TTGTAGAAGA ATATGCTATT 
TTAAATAAAC TTGTACGTTT ATTAGTCGTA AGTGAGGGTC CGTTAACTGC AAGTAAAAGT 
AAAATTGTTA AGGAAGGTAA TAAGGATACA TCATCTACGA AAACTAGAAA ATGGCTTGCA 
CCTTTGTTAT TGCTGATAGA ACGTTTAGAA AAAGTAGCGG TATTGACAAA GAGAAAAGAA 
TTAATGTATA AAGTAACAAC CAGGATGTGG AAATGGTTCG ATCTCAGCAC AGGAAAATGG 
AATTTATATT CTCCAGTAAA TAATCGAATA ATTAATGATG CGTATTGGGC TGGCGAGTCT 
AGTACGAGAG TAACATGTAA CAGGCGACGA TATGTAATTA ATTTTTCATG CATGACTCAA 
GTTAATGAAG AAACTGGTAA TAGAAGACCT ATTACGATGG GCTTTAAATT GCATACCGGA 
AATGAAGATG GTGGATCAGG TTCTGATACA AATATGGACA CTGACGAAAA TCGAATAGAA 
GATAAACGGA ATACAGTTTT GCAAGGGTTA GATCCTAGCA TGGCTCCAAG TATCATACGC 
GCTTGTGTAA GATTGATGGC TATTCCAGTA GATCGAGATG CTTTACATGC AGCTATGAAA 
GTTTGTTTGA GACTTACAAG AGATTATGAA AATGCCGAAA TATTTGTTCG TGAGGGTGGT 
GTGAAACTTC TTCTAGAAAT GTCTCAAGCA AGTAGTTTCA TTGGATGCAT AACTTTAAGT 
ACTCTTTTAA TAAGGCATGC TTTGGAAGAA CCATGTACTC TTCGATTAGC CATGGAAAAA 
GTAATTCGTA GTCGTACTCA AGGCAATATT CCTCCAGCTT GCAAAGAAAT CTTATTTATG 
ACACGTCAGA TTAATAGCGC TGTATGTCGC AATCCGGAAG TTTATAGAGA AGTTTGTGAA 
AACATTTTAA GAGTAGATAT TCGTCTATTA GGAAAAGAGG ATGATGATAA TAGATTAATT 
GTCAAGGCAC TTCCACCAAG TCACTCATCA GTAATGCCTA TGGTAGAGAA AGCTTCAATA 
TCTGTTGTAA AAGATCTTCT GAATGCTCTT ATAAAGCCTA TGCCAGTTGT CTCTGATGAA 
AAGTCTTCTA CACCAACAAG GAGTCCAGAA AAGAAGACAT CTTATTCTAA TGTTGGAAGT 
TCATCTTCTC GACGAGATGT TCTTAGAAAT AATGCAACAA CATCAAATGA TACTCTTGAT 
GATGAGGATC AAGTTTCTCA AATAATACCA ATGAAAATTT ATCCAGATAA TAGCAATAAC 
AGTAAAGTGG AACCAGAAGA AGCAGAAAAA CCTTTATTAA CAAAATCTGC TATTTTGAAG 
ATACTTGCAG AAGCCGTTCG CTCATATAGT GCAGTCGCAA GTTTAATTAC AGAGCATGCA 
TATCGAGCCG GACAAAGTGA AATGGTTCCT GAATACACAA CAGCTCTTGC TTTTCTTTTA 
GATAAGCTTT TACCAATGTC ACCAGAAAAT TCAAGTGACA AACAATGCAG CAGTATGGCG 
AGAATGTTAA TAGCTGCTAT TGCTTCCTGT AATCATTCTC CGGATGTTCA AACAACTTTG 
GTGACTGAAG TAAAAGCAGC TTTAACAAGA ACTTTAGCTC TTCCTGAAAG TTCTGAGAAA 
CATGCACAAA TTCAATTATT AGTTGGATTG ATCTCTACTA TGATTGATAG TTGTCCACCA 
ACTTCTTACA ATCAATTGAG GACTTCCTTG AAGATGCATC AATACAATAA TGTTAACTAT 
ATTGTAAGAA TCATGTTGAG AAAAGGAATA ATTACAGATT TAGCAAAAAT TCCACATAGT 
CTGGATTTAT CAAGTCCAAA TATGGCTGCT ACCATAAATG CTGCTTTAAA ACCATTGGAA 
ACGCTTTCGC GAATTATAAA TCAACCAATG CCAGGTGCTA TTAATAACAA ATTTACAAAG 
CCAAAAAGTA GAACAGTTCA AGAGGAACCT ATTGGAGAAC AAACAGGAAC TACAACATCA 
GAAGCGACAC ATGCTGTTGG CGAAGAAACG AACGAAGACG CAGAGAATAC AGAACACGAT 
ATATCTGTTA CTGCGGAAAG TCTTGAACCT ACTTCAGAAA GTCAAGTACA TGAAGATGAA 
GCTGCTTTAG AAGACATTAT GGATCAACTG CTTGAAAGGG ACGGGCCGGG CGTGACGGAA 
GATCAATCTT CTCGACCACA TAGACCAATG GACATTGATG AAGAAAGCCT TGCAATAAGA 
GATGGAGAAG CTGATTTTGA TCCAACACGA GATGCTACGG AGGATTTAAT GAGCTCTGAT 
TCGGATTCTG ATAGCAATCC ATCTGATGAT AATGAAGATG AAGTGCAAGA TGATGAAGAT 
GAAGAAGAAG AAGAAGAGGA TGATGGAGAA GAAAATGAAG AAGAAGAGGA AGAAGAAGAA 
GAAGGATCCT CAAATTACGA AGAAGACGGA ATTGAATTCT ATGAAGATGT AGGAGAAAGT 
GGTGTATTCA GAATGATTCC ATCAACAGAT CGTGATGGTA GTAGTGTCGT AATGATTCGA 
CATAATATAG ATAATCAAGA CAATGTGAAT TCTTCAACTA CAGTTACACC ACGATCTAGT 
CTTCCATGGA GTACAACATT TCCTCTTCCT TTGGGACTAT TTGAAAATCC TCCATCAATT 
TCTGAAAGTA GTGGTATTAA TTCCCATTCG TTGCTAATAC CACAGGGAGG TTTAGATGCT 
ACTAATACAA GAGGTCAGAG AGCTGTACGA CCGCGAAGAT ATCAATATCT TCAATTACCA 
AATCCACGAA ATCCAAATCC ACCTGTAATA TTGCAAAGAC TTTTAGGTCC AGCTGCACAA 
GCAATTCCAT TATCTGCCAG TCAGGTTTTG GCTTCCAGTT TTCGAGATAC ACGCGTTGTA 
GTAATGGATA ATGGTGTTGG AATATTAACA AATCAAGAAG AAGAACAAAT AGATTTTGTG 
GATCAATCTG GCTATCTTTT CGGATCTAGT CTTGCAACCA CAGTAAATAA TACTCCTACT 
GCTTTACATT GGTGGATTGA AGAAAGCAAA CTATTAGATG GAGATTCCCA AACGGATTGC 
TGTGTTGGTG TTGTACATAA ACTAATTCCT GGATTGGAAA AACGTAGAAA TGCAGAATTG 
GGAGAACGAC GTGAAAGACG TAAAATGCAA TACGCTTCTG AGAAAAAAAA TGAAAAAGAT 
AACAAAGATG GAAAAGATCA TTTAAATAAT ACAGAATCTG GTTCTTCTAC TATAATATCT 
ATGGATGAAC AAATATCAAC TGCATCAACT TCACAAAGAG AAGAAACGAA TAATTTTGTT 
CCTAGATTTG AAACTTGCGT GGAAACAACT CGATTAGAAC GCACACTTGA CGAAGATATA 
ATTGATGTAA CTGGTGAGAT GGATGCAACC ATTCAAGAAG ATGAAGAACG ACCACCACCT 
CCCCCACCAG AAGAACAACC AACAGAACCT AGAGATCCTA GAATAAGCAA TCGAAATCCA 
ACTTTGGAAC TTGCTGAAAG TATAGTGGAT TCTGTACTTG GACCTTGCGC GTCTGAAGCA 
AGCAGATTAA GACAATCTGA ACGTACCGTT GAAGGATCTA ATACTAATGA AACATCTAGT 
CGACCCAATA CGTCCATAGT TGTTGATTTC AGTCAAGAAC CTAATGAAGA TCTATTAAGA 
GAAAGTGATT CATCTGAATG GATAAGAATT CTTACGGATG ACAGTCAATC AAATGTTGAA 
CGAAATGCGA ATATTCTCAA TCAAGTACCT ACCACTTCCG CATCAGAAAA TATTAATCCA 
GAAATATCAG AACAACAACA GCAACAGCAG CAGCAACAAC AACCATCTAT ACAACAGCAG 
CAACAACAAG AGCCACAACA ATCACAAGAA CAGTCGCAAC GACAACAACA AAACCAACAA 
TCTCAAGAAC AACAATTACC GGATGGTGTA GATCCTTCTT TCTTAGCTGC ATTACCCGAA 
GATATGCGTG ACGAAGTTAT TGCTGAGCAA CTAAGACTTC AACGAATTCG GCAAAGAGCA 
CAGGCAACTG TAGTCGAAGA ATTAACAGGA CCGGTTGAAG TTAATCCTGA ATTTTTAGCT 
GCTTTACCTC CAGCAATACA AGAAGAAGTT CTTGCGCAAC AACGCTTGGA ACAACAACGT 
CATGCAGCCG CAAGTGCCAA TCCAGAAGAC CCAGTTGACG CAGCTGCATT CTTCCAAAAT 
TTACAACCTT CTCTTCGACA AGCGATTTTA ACTGATATGG AAGAATCGCA AATATCCGTT 
CTTCCACCAG ACTTAGCTCA AGAAGCTCAA AATCTTCGAA GAGAATGGGA AGCTCGAAAT 
AGACATATAA TGCAAGATCG ATTTCTTAAT CATGTTAGTC AAGGAAATGC CGCCTTATCT 
AGTATATTGC GAAATTCTGG AAGAGGTAGA GGTGGTGGAA CGCGTTATGC AATTCATACA 
GTAGCACAAA GAGGATGGCA ACCATGGTCA AATGGTGATC CTAGTATAAC GCATCATGGA 
GCGGGTCTAC GACTCAGAGG CAGACAATTA TTGGATCATG AGTCGATGGC TTGCGTATTG 
GTATTACTGT TTGTTGATGA ACCAAAAATT AATACATTAA GGCTTCATAG AGTTATCAGG 
AATCTTTGCT ATCACGGTAG CACAAGGGAA TGGGTAGTTA GAGCTTTACT AAGTATCATG 
GAAAGAAGTA ATGATGAAAA TAAAGATATA GCCATGGACT TTGGTGGAAA AATCAGAAGA 
AAAAGCTTAG TGCCCATGAT GCATCACGAT TATTGTGCTC CTAAAATTTT TGATCAAAGA 
AGCAATGCAC AGTCATGGTT AAACATCAGT ATTGATGCTG CGCTTGGATG TAGAGCAAAT 
GTTTTCCATA TTATTAGACA TGGAGGCAAA AAATCAGAAA GACAAGGCAG TAGTATTGCC 
ATCCATCCTC AAGCAGCTCC TACAGTATGC AGGCATACAT TAGAATTATT AATATCATTG 
GCTAAAGGCT TTCATAATAT CACAACTTTT GAGTCATCCG CTTTCGGTCA ATTAATTTCA 
ATGCTAAACT GGTCGGTTAT TAAACGTAGC AGTCAATTAA CGGACAAAGT GTTGAAATTA 
TTGTCTTTAA TTTCAATCGG ATTAACGGAA GTGAATCCGT ATCGTAGACA AGAAGGAAAT 
AAGAATAAAA GAACAGATGT GAATAAAGAA CAAAGTATTG CTGCACCAGA AGAACATCTT 
AAATTGGCTG TGCAAGTATT GACAAGTAAG AGTTGCTCTG AAGAAGGACT TGAAGATGCA 
ACAGCTTTGT TGCTTAATTT ATCTCATTGT CCTGATCCTA CTAGACAATT GATATTAAAA 
TTACTCTTAG AAGGTGCCAT GGAATTGGCT AATGTTGTTT GTGAACACAT TAAAGATTTA 
TTAATGGAGT TAAAAGTTTT GAATCGTGAA CTTAGACGAA ATTCTATCGA AGAGCAACCG 
TCAACTAGTA CTGGAGGTTC TCGTTGGGTT CTTTTAGATA GGTTTACCAA CGAGAGTGTA 
GTTATTACTG CGCCGAGTAA AGTTAAAGCT GGCAGCGATC TTCAATTACC ATCGATGGCT 
GCTCTTGTTA GCAAAACTTC TAGTCAGGCA TTTTTCTTAA GAATACTTAA AGTGATTGTA 
CAAATAAGGG AAGCTGTACG TTTAGCAAAT AATAAAAAAA CATCCACTCA AAGTTCCGAA 
AGTTCTGTAG ATACTGAAAA TACTAATCAA GCTTCAGAAT CAGGTAATTT GAAGCTTTAT 
ATTTTTATAA ACATAGCTTA ATTTTCACAA TTAAATAATA TTTGTATACA ATTTATAGGT 
GAAGATAAGC TTCCATTATT AAGTGAAAGT TTAGTATTAG ATCATCTATG GGAAACACTT 
AGTGCTTGTT TATTGGAATT GGAATATACT CCAGATCAAC ATGCAGTTTT AGTATTGCAA 
CCTGCAGTAG AAGCATTTTT CCTAGTACAT TCATCATCGA GTACTTCTCG TGATCGTCAT 
CTAAGTACAA GTACGGAAAA TCGCGAAGTA GTTCCTGATC TAACACCTGT TTCACCAATT 
TATTCAGATA ATGAAGGAAC GTCCCAATCA GAAGCTACTA TTAATACTTC TTGGGATATA 
CAACCTACGC CACAGAAGAC ATTACCACCG GACCAACTCA AGTTCCTTAA ATTTGCAGAA 
ACGCACAGAA CCGTTTTAAA TCAAATTCTT CGTCAAACAA CTACGCATTT AGCAGATGGA 
CCATTCTCTG TGTTAGTGGA TCACACAAGA GTTCTTGATT TTGACGTAAA AAGACGATAT 
TTCCGAACAG AATTAGAACG AATGGATGAA GGTATTCGAA GGGAAGAATT GGCAGTACAT 
GTTCGTAGGA ATAATGTATT TGAAGATTCA TTTCGAGAAC TTCATAGGCG TAATGCTGAT 
GAATGGAAAA ATCGTTTTTA TATTGTTTTT GAAGGTGAAG AAGGGCAAGA TGCTGGAGGA 
TTACTTAGAG AGTGGTACGT TATTATTTCC AGAGAAATTT TTAATCCTAT GTATGCATTG 
TTTACTGTAA GTCCTGGCGA TCGAGTTACA TACATGATAA ACTCCTCTTC ACATTGTAAT 
CCTAATCACT TATGTTATTA TAAATTTGTC GGTCGAGTTA TAGCTAAAGC AATTTATGAC 
AACAAATTAT TAGAATGTTA CTTCACGCGT AGTTTTTATA AACACATTTT GGGAATACTT 
GTGAAACATA CAGATATGGA AAGTGAGGAT TATAGTTTTT ACAAAGGACT TGTATATCTC 
ACAGAGCATA ATATTGCTGA TCTTGGATAC GAATTAACTT TCTCTACAGA GGTAAACGAA 
TTTGGTGTAA ATGATGTACG TGACTTAATA CCAAATGGAC GTAATATCAT TGTTACTGAG 
GAAACAAAAT TAGAATATAT TCGGCTCGTA TGTCAAATGA AAATGACTGG AGCAATTCGT 
AAACAATTAA ATGCGTTCCT AGAAGGTTTT TATGATATTA TTCCCAAACG ACTGATCTCT 
ATATTCAATG AACAAGAACT TGAATTGTTA ATTAGTGGAT TACCCAATGT AGATATTGAG 
GATCTTAAAG CTAATACTGA ATATCATAAA TATAATGNNN NNNATATTTA GATTCAATGG 
TTCTGGAGAG CATTACGAGG TTTCGATCAA GCAGATAGGG CTAAATTTTT GCAATTTGTT 
ACTGGAACTT CAAAAGTGCC ACTTCAAGGT TTCGCTGCTT TAGAAGGAAT GAATGGTATA 
CAAAAGTTCC AAATTCATCG TGAAGATAGA TCGACAGATA GACTTCCGTC AGCACATACA 
TGTTTCAATC AGCTAGATCT ACCAGTTTAT GAAACATACG ATAAGCTTCG CACGAATTTA 
TTAAAAGCGA TCCACGAATG CAGTGAAGGA TTCGGTTTCG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016768793.1
CDS	900..13184
Translation	MKIDRNRLRKYTSEAPAKCQALINKLRSCSHAELLEELKQIDAWTFGKCELFHWVDVLDLSDSILEEAAAKSPDNPWELACDLPQNREAKELLLWVLHLTTLLIEHSYSRHLYSSMEHLVTLLSSCDMHVVLGVLNILYMFSKRSNFIARLNSDKRQALLSRLTLLAESWGGKENGFGLAECCKEYPDKPLPASATTLHFEFYAENSTTDSNATSNIGTKKSGQTNFVTCIHIENVDKLGKTPAQIMNDLLKVNNVPQDRQIALFTHIRLAHSFSDYRRRLQCVHARLQALSILVYNSELEENAHSLLHPGLLEELVELLELPHAHLVELRAAALRTLTSIIHLDRNPHFPKKPSSRLNMIIDVTGANSYHGFFPVLIRTCISTLISPQPEGQPFPLSLATALFSFLYHLSSYEAGCEALVSCGMMESLLKIMNWPGSEFEHLTFVTRAVRVIDLITNIDMQGFQAHGGLASFINRLDMEVNVCRKEQPFEIEPIHYQDNSQATRERSMDRDESSTSSRAFDEREESSNPMEFSEDENMSSKTDDKNEQIPDYSAGKTGKTCLPQRVTLLKALLNFIKKIIQDPTFSDSIRHVMEGTLPSSLRHIISNSEYYGPSLFLIATDAVTVYVFQEPSLLSSLQDNGLTDLVLHALLIKEIPASREVLGSLPNVFSALCLNQRGLESFVKRRPFERLFKVLLSPLYLPAMNRRRSTDPLGDTASNLGNAMDELMRHQPSLKVDAIAAVIKLLEELCVLGCDPRYICLQAEDKFDNSQSNTTSGNRQSSGQSVGVGVGDSGVGGGGGSGSSSDEEEEDEEEASTSSHPQREEQCSSSTAQPVPPPQPREKTPIALMEYIQNVTKFVDAILSNNSTDDHCREFVAQKGLVPLLSILGLPNLPVDHSVAQTAQAVATVCKSILNLAHEPLVLKTGLSQLNEVLNLLKPLYNYMEAPGGSVLLHELVNAPNLETAFTSDTATPLLHAMNAAYGYIIMFAQVCRTSQSEIRNLAMNHWGSELGLTVLKGLSELYTALIWESTALLALTTEDFIPAGCDFGKEDMDKLIRPESTNEGESTSWSGATSTDMGSNGMTTAMEALSTDPPPIPMEVDEKPSVSTGRISPNSHRLTYPKHLLGLTSKMGRALAELFAILVKLCMGSPIRQRRGQQMPPIPALPSQASRSVATALNCVLIRGLSWDKLPPSTVPKLQVTFLMCTLSFVSPALFDEKGYPFHLMLQKFVKLGGQNAFFAAFHWVLSGGGQFPLSEGLEHPNLPDCTGEFLDGWLMILEKMVNPKTLLDSPYVVSSKCTGIYKVYETFDPIKYLTDIHKEAFEAVMLMWGKKPLKNYGVHMTELILSILRHILRSEQIIKEHAEKEENNEGGISGSTSGTSGIGRGGTISDRREEPEADVNPEHLRQLMDMGFSRAHCIEALLHTLTVEQATDYLLTNPATLRRSDVPAGDVSGQDLIMDLELVDDDQIMHAIAMSLGETAETRNVFSTEESSSREATTITESIDKFTQNALSQCLNLLDLMPDTVYRICDLLVAIAKRNGDEWRDNMLRQLMKEIGGLVDYLIEVAESQDPNAGVKLVECKEANKVTGRIYLYTMFFEGTFQEMRVPCAQIVEEYAILNKLVRLLVVSEGPLTASKSKIVKEGNKDTSSTKTRKWLAPLLLLIERLEKVAVLTKRKELMYKVTTRMWKWFDLSTGKWNLYSPVNNRIINDAYWAGESSTRVTCNRRRYVINFSCMTQVNEETGNRRPITMGFKLHTGNEDGGSGSDTNMDTDENRIEDKRNTVLQGLDPSMAPSIIRACVRLMAIPVDRDALHAAMKVCLRLTRDYENAEIFVREGGVKLLLEMSQASSFIGCITLSTLLIRHALEEPCTLRLAMEKVIRSRTQGNIPPACKEILFMTRQINSAVCRNPEVYREVCENILRVDIRLLGKEDDDNRLIVKALPPSHSSVMPMVEKASISVVKDLLNALIKPMPVVSDEKSSTPTRSPEKKTSYSNVGSSSSRRDVLRNNATTSNDTLDDEDQVSQIIPMKIYPDNSNNSKVEPEEAEKPLLTKSAILKILAEAVRSYSAVASLITEHAYRAGQSEMVPEYTTALAFLLDKLLPMSPENSSDKQCSSMARMLIAAIASCNHSPDVQTTLVTEVKAALTRTLALPESSEKHAQIQLLVGLISTMIDSCPPTSYNQLRTSLKMHQYNNVNYIVRIMLRKGIITDLAKIPHSLDLSSPNMAATINAALKPLETLSRIINQPMPGAINNKFTKPKSRTVQEEPIGEQTGTTTSEATHAVGEETNEDAENTEHDISVTAESLEPTSESQVHEDEAALEDIMDQLLERDGPGVTEDQSSRPHRPMDIDEESLAIRDGEADFDPTRDATEDLMSSDSDSDSNPSDDNEDEVQDDEDEEEEEEDDGEENEEEEEEEEEGSSNYEEDGIEFYEDVGESGVFRMIPSTDRDGSSVVMIRHNIDNQDNVNSSTTVTPRSSLPWSTTFPLPLGLFENPPSISESSGINSHSLLIPQGGLDATNTRGQRAVRPRRYQYLQLPNPRNPNPPVILQRLLGPAAQAIPLSASQVLASSFRDTRVVVMDNGVGILTNQEEEQIDFVDQSGYLFGSSLATTVNNTPTALHWWIEESKLLDGDSQTDCCVGVVHKLIPGLEKRRNAELGERRERRKMQYASEKKNEKDNKDGKDHLNNTESGSSTIISMDEQISTASTSQREETNNFVPRFETCVETTRLERTLDEDIIDVTGEMDATIQEDEERPPPPPPEEQPTEPRDPRISNRNPTLELAESIVDSVLGPCASEASRLRQSERTVEGSNTNETSSRPNTSIVVDFSQEPNEDLLRESDSSEWIRILTDDSQSNVERNANILNQVPTTSASENINPEISEQQQQQQQQQQPSIQQQQQQEPQQSQEQSQRQQQNQQSQEQQLPDGVDPSFLAALPEDMRDEVIAEQLRLQRIRQRAQATVVEELTGPVEVNPEFLAALPPAIQEEVLAQQRLEQQRHAAASANPEDPVDAAAFFQNLQPSLRQAILTDMEESQISVLPPDLAQEAQNLRREWEARNRHIMQDRFLNHVSQGNAALSSILRNSGRGRGGGTRYAIHTVAQRGWQPWSNGDPSITHHGAGLRLRGRQLLDHESMACVLVLLFVDEPKINTLRLHRVIRNLCYHGSTREWVVRALLSIMERSNDENKDIAMDFGGKIRRKSLVPMMHHDYCAPKIFDQRSNAQSWLNISIDAALGCRANVFHIIRHGGKKSERQGSSIAIHPQAAPTVCRHTLELLISLAKGFHNITTFESSAFGQLISMLNWSVIKRSSQLTDKVLKLLSLISIGLTEVNPYRRQEGNKNKRTDVNKEQSIAAPEEHLKLAVQVLTSKSCSEEGLEDATALLLNLSHCPDPTRQLILKLLLEGAMELANVVCEHIKDLLMELKVLNRELRRNSIEEQPSTSTGGSRWVLLDRFTNESVVITAPSKVKAGSDLQLPSMAALVSKTSSQAFFLRILKVIVQIREAVRLANNKKTSTQSSESSVDTENTNQASESGNLKLYIFINIAFSQLNNICIQFIGEDKLPLLSESLVLDHLWETLSACLLELEYTPDQHAVLVLQPAVEAFFLVHSSSSTSRDRHLSTSTENREVVPDLTPVSPIYSDNEGTSQSEATINTSWDIQPTPQKTLPPDQLKFLKFAETHRTVLNQILRQTTTHLADGPFSVLVDHTRVLDFDVKRRYFRTELERMDEGIRREELAVHVRRNNVFEDSFRELHRRNADEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFTVSPGDRVTYMINSSSHCNPNHLCYYKFVGRVIAKAIYDNKLLECYFTRSFYKHILGILVKHTDMESEDYSFYKGLVYLTEHNIADLGYELTFSTEVNEFGVNDVRDLIPNGRNIIVTEETKLEYIRLVCQMKMTGAIRKQLNAFLEGFYDIIPKRLISIFNEQELELLISGLPNVDIEDLKANTEYHKYNnullnullnullIIQWFWRALRGFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHREDRSTDRLPSAHTCFNQLDLPVYETYDKLRTNLLKAIHECSEGFGFA

Target ORF information:

RefSeq Version	XM_016913304.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera E3 ubiquitin-protein ligase HUWE1 (LOC411961), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016913304.1

ORF Insert Sequence:

ATGAAAATTG ATCGCAATAG ATTGAGAAAA TATACATCAG AAGCGCCTGC AAAATGTCAG 
GCGCTTATAA ACAAGTTGCG CTCATGCTCC CATGCAGAAT TGCTAGAGGA GCTAAAGCAA 
ATAGATGCAT GGACTTTTGG TAAATGTGAA TTATTTCATT GGGTCGATGT ATTGGACCTT 
AGTGACAGCA TTTTGGAAGA AGCTGCTGCT AAAAGCCCAG ATAATCCATG GGAATTGGCT 
TGTGATCTCC CTCAAAATAG AGAGGCAAAA GAACTTCTCC TTTGGGTTCT ACATCTTACA 
ACATTATTGA TTGAACATTC ATATTCGCGA CATTTATACA GCAGTATGGA ACATCTAGTG 
ACTTTATTAT CAAGCTGTGA CATGCATGTT GTCCTTGGTG TATTAAATAT TCTCTACATG 
TTCAGTAAAC GTAGCAATTT CATTGCACGT TTAAATAGTG ACAAGAGACA AGCTTTATTA 
AGTAGACTTA CTCTTTTAGC AGAGAGTTGG GGAGGTAAAG AAAATGGATT TGGTTTAGCA 
GAATGTTGTA AAGAATATCC AGATAAGCCA CTTCCAGCAA GTGCCACAAC ATTGCATTTC 
GAATTTTATG CAGAAAATTC AACGACAGAT TCAAATGCCA CATCAAACAT TGGCACAAAA 
AAATCTGGAC AGACGAATTT TGTAACATGT ATACATATAG AAAATGTAGA TAAACTGGGT 
AAAACACCAG CACAAATTAT GAATGATTTG TTAAAAGTCA ATAACGTACC CCAAGATCGA 
CAGATTGCGT TATTTACACA TATAAGATTA GCACACAGTT TCTCTGACTA TAGAAGACGA 
TTACAATGTG TGCACGCTCG GCTACAGGCA TTATCAATAC TTGTATATAA TAGTGAACTA 
GAAGAAAATG CACATAGTTT GTTGCATCCT GGACTTTTAG AAGAATTAGT TGAATTACTG 
GAACTTCCAC ATGCACATCT TGTTGAATTA CGTGCTGCTG CTTTAAGAAC TCTTACAAGT 
ATCATTCATT TAGATCGCAA TCCACATTTT CCAAAAAAAC CTAGTTCAAG ATTAAATATG 
ATAATCGATG TCACTGGAGC CAATTCATAT CATGGATTTT TTCCTGTATT AATTCGAACT 
TGCATTTCAA CATTAATTTC TCCACAACCT GAAGGACAAC CATTTCCTTT GTCACTTGCA 
ACAGCTTTAT TTAGCTTTCT TTACCATCTT TCAAGTTATG AAGCAGGATG TGAAGCACTT 
GTTAGTTGTG GGATGATGGA AAGTTTGTTA AAAATCATGA ATTGGCCTGG TAGCGAGTTT 
GAACATTTGA CGTTTGTAAC AAGGGCAGTT CGTGTTATAG ACTTAATAAC AAATATAGAT 
ATGCAAGGAT TTCAAGCTCA TGGTGGTTTA GCCAGTTTTA TTAATCGTCT TGATATGGAA 
GTAAATGTTT GTAGAAAAGA ACAACCATTT GAAATAGAAC CAATACATTA TCAAGATAAT 
TCTCAGGCCA CTCGTGAAAG ATCAATGGAT CGCGATGAAA GTTCTACGTC TTCACGTGCT 
TTTGATGAGC GTGAAGAATC ATCTAATCCT ATGGAATTTT CGGAAGATGA GAACATGAGT 
TCCAAAACCG ATGATAAAAA TGAACAAATA CCAGATTATT CAGCTGGAAA AACTGGAAAA 
ACTTGTCTAC CACAGCGAGT TACACTTTTA AAAGCGTTAT TAAATTTTAT TAAAAAAATT 
ATTCAAGATC CTACATTTTC TGATAGTATT AGACATGTTA TGGAGGGTAC TTTACCTTCT 
TCCTTGAGAC ATATTATCAG TAATTCAGAA TATTATGGTC CTTCTTTGTT CCTTATTGCA 
ACAGATGCTG TAACAGTATA TGTTTTCCAA GAGCCATCTT TATTATCTTC TTTACAGGAT 
AATGGATTAA CTGATCTTGT ATTGCATGCT TTACTAATTA AAGAAATTCC TGCTAGTAGA 
GAAGTTCTAG GATCATTGCC TAATGTATTT AGTGCTTTAT GTTTAAATCA GCGTGGACTA 
GAATCATTTG TAAAACGACG TCCTTTCGAA CGCTTATTTA AAGTATTATT ATCGCCACTT 
TATCTGCCTG CAATGAATCG ACGTCGTAGC ACAGATCCTT TAGGTGATAC TGCTTCGAAT 
TTGGGTAATG CAATGGATGA ATTAATGCGA CATCAGCCAA GTTTGAAGGT TGATGCAATC 
GCTGCTGTTA TAAAGTTATT AGAAGAACTT TGTGTACTGG GTTGTGATCC TCGTTATATA 
TGCTTGCAAG CTGAAGATAA ATTCGATAAT TCTCAATCAA ATACTACTTC TGGAAATCGT 
CAATCTTCTG GACAATCTGT TGGAGTTGGA GTTGGAGATT CTGGTGTAGG AGGAGGAGGT 
GGTAGTGGAA GTAGTAGCGA CGAAGAAGAA GAAGATGAAG AAGAAGCTAG TACAAGTTCT 
CATCCACAAC GTGAAGAACA ATGTTCATCA TCCACTGCTC AACCAGTTCC ACCACCTCAA 
CCTAGAGAAA AAACTCCAAT AGCTTTAATG GAATATATAC AAAATGTTAC AAAATTCGTT 
GATGCTATTC TCAGTAATAA TTCCACGGAT GATCATTGTA GAGAATTTGT CGCCCAAAAA 
GGTCTCGTAC CATTATTGTC AATTTTAGGA TTACCTAATC TTCCAGTGGA TCATTCAGTT 
GCCCAAACGG CACAAGCTGT AGCTACAGTT TGTAAAAGTA TTTTAAATCT CGCACATGAA 
CCATTAGTTC TTAAAACTGG TCTATCTCAG TTGAATGAAG TTTTGAATTT GCTGAAACCA 
TTATACAATT ATATGGAAGC TCCAGGAGGA TCTGTTTTAT TACATGAACT TGTCAATGCT 
CCAAATCTGG AAACAGCTTT TACCAGTGAT ACGGCAACAC CTTTACTTCA TGCTATGAAT 
GCTGCTTATG GATATATAAT AATGTTTGCA CAAGTGTGCC GTACCAGTCA AAGTGAAATC 
AGAAACTTGG CTATGAATCA TTGGGGTTCT GAACTTGGTT TGACTGTTCT GAAAGGTTTA 
TCAGAATTAT ATACAGCTCT AATCTGGGAA AGTACAGCCT TATTAGCACT TACCACGGAA 
GATTTTATTC CGGCTGGTTG TGATTTTGGA AAAGAAGATA TGGATAAACT TATACGTCCT 
GAATCAACAA ATGAAGGTGA AAGTACTAGT TGGTCTGGTG CTACATCTAC AGATATGGGA 
AGTAATGGGA TGACTACAGC TATGGAAGCT TTAAGCACTG ATCCGCCTCC TATACCTATG 
GAAGTAGACG AAAAACCAAG TGTTAGTACA GGAAGAATAT CTCCAAATTC TCATCGACTT 
ACATATCCTA AACATTTGCT GGGACTTACA TCTAAAATGG GCAGAGCTTT AGCAGAACTC 
TTTGCTATAC TTGTAAAGCT TTGCATGGGC TCTCCAATTA GACAACGCAG AGGACAACAG 
ATGCCTCCAA TACCAGCCTT ACCTTCACAA GCATCTAGAA GTGTAGCAAC TGCTTTAAAT 
TGTGTATTAA TTCGCGGATT ATCATGGGAT AAATTACCAC CATCGACAGT GCCAAAACTT 
CAAGTAACCT TTTTGATGTG CACTTTAAGT TTTGTATCTC CTGCATTATT TGATGAAAAG 
GGGTATCCAT TTCATTTGAT GCTTCAAAAG TTCGTTAAAC TCGGCGGACA AAATGCATTT 
TTCGCTGCTT TTCATTGGGT ATTATCTGGA GGAGGACAGT TTCCGTTATC TGAAGGATTA 
GAGCATCCAA ATTTACCTGA TTGTACTGGA GAATTCTTAG ATGGTTGGTT GATGATTTTA 
GAAAAAATGG TGAATCCTAA AACTCTTCTT GATTCTCCAT ATGTTGTTTC TTCAAAATGT 
ACTGGAATAT ATAAAGTATA TGAAACATTT GATCCTATAA AATATCTTAC GGATATTCAT 
AAGGAAGCTT TCGAGGCAGT GATGCTAATG TGGGGAAAGA AACCTTTAAA AAATTATGGA 
GTTCATATGA CAGAACTTAT TTTATCTATC TTAAGACATA TTTTACGAAG TGAGCAGATC 
ATAAAGGAAC ATGCTGAAAA AGAAGAAAAT AATGAAGGAG GTATTAGTGG TAGTACTAGT 
GGAACTAGTG GAATCGGAAG AGGTGGAACA ATATCCGATC GCAGAGAAGA ACCAGAAGCA 
GATGTAAATC CAGAACATCT AAGACAGTTG ATGGATATGG GTTTCAGTAG AGCTCATTGC 
ATAGAAGCTT TGCTTCATAC ATTGACAGTT GAACAAGCTA CTGATTATTT GTTAACTAAT 
CCTGCTACTC TACGTCGATC AGATGTGCCA GCAGGTGATG TAAGCGGACA AGATTTAATA 
ATGGATTTAG AATTGGTAGA TGATGATCAA ATAATGCATG CCATAGCGAT GTCGCTTGGA 
GAAACGGCGG AAACGCGAAA TGTTTTCAGC ACAGAAGAAT CCAGTTCACG TGAGGCTACT 
ACCATAACTG AAAGCATAGA TAAATTTACG CAAAATGCTT TAAGTCAATG TTTAAATTTA 
TTGGATCTCA TGCCTGATAC TGTATACAGA ATTTGCGATT TATTAGTTGC CATCGCTAAA 
AGAAACGGAG ATGAATGGCG TGATAATATG TTGCGACAAC TTATGAAAGA AATTGGAGGT 
TTGGTAGATT ATTTAATCGA AGTTGCTGAA AGTCAAGACC CGAATGCTGG AGTAAAATTA 
GTAGAATGTA AAGAAGCTAA TAAAGTTACT GGACGTATTT ATCTGTATAC AATGTTCTTT 
GAAGGAACAT TCCAAGAAAT GCGAGTGCCC TGTGCTCAAA TTGTAGAAGA ATATGCTATT 
TTAAATAAAC TTGTACGTTT ATTAGTCGTA AGTGAGGGTC CGTTAACTGC AAGTAAAAGT 
AAAATTGTTA AGGAAGGTAA TAAGGATACA TCATCTACGA AAACTAGAAA ATGGCTTGCA 
CCTTTGTTAT TGCTGATAGA ACGTTTAGAA AAAGTAGCGG TATTGACAAA GAGAAAAGAA 
TTAATGTATA AAGTAACAAC CAGGATGTGG AAATGGTTCG ATCTCAGCAC AGGAAAATGG 
AATTTATATT CTCCAGTAAA TAATCGAATA ATTAATGATG CGTATTGGGC TGGCGAGTCT 
AGTACGAGAG TAACATGTAA CAGGCGACGA TATGTAATTA ATTTTTCATG CATGACTCAA 
GTTAATGAAG AAACTGGTAA TAGAAGACCT ATTACGATGG GCTTTAAATT GCATACCGGA 
AATGAAGATG GTGGATCAGG TTCTGATACA AATATGGACA CTGACGAAAA TCGAATAGAA 
GATAAACGGA ATACAGTTTT GCAAGGGTTA GATCCTAGCA TGGCTCCAAG TATCATACGC 
GCTTGTGTAA GATTGATGGC TATTCCAGTA GATCGAGATG CTTTACATGC AGCTATGAAA 
GTTTGTTTGA GACTTACAAG AGATTATGAA AATGCCGAAA TATTTGTTCG TGAGGGTGGT 
GTGAAACTTC TTCTAGAAAT GTCTCAAGCA AGTAGTTTCA TTGGATGCAT AACTTTAAGT 
ACTCTTTTAA TAAGGCATGC TTTGGAAGAA CCATGTACTC TTCGATTAGC CATGGAAAAA 
GTAATTCGTA GTCGTACTCA AGGCAATATT CCTCCAGCTT GCAAAGAAAT CTTATTTATG 
ACACGTCAGA TTAATAGCGC TGTATGTCGC AATCCGGAAG TTTATAGAGA AGTTTGTGAA 
AACATTTTAA GAGTAGATAT TCGTCTATTA GGAAAAGAGG ATGATGATAA TAGATTAATT 
GTCAAGGCAC TTCCACCAAG TCACTCATCA GTAATGCCTA TGGTAGAGAA AGCTTCAATA 
TCTGTTGTAA AAGATCTTCT GAATGCTCTT ATAAAGCCTA TGCCAGTTGT CTCTGATGAA 
AAGTCTTCTA CACCAACAAG GAGTCCAGAA AAGAAGACAT CTTATTCTAA TGTTGGAAGT 
TCATCTTCTC GACGAGATGT TCTTAGAAAT AATGCAACAA CATCAAATGA TACTCTTGAT 
GATGAGGATC AAGTTTCTCA AATAATACCA ATGAAAATTT ATCCAGATAA TAGCAATAAC 
AGTAAAGTGG AACCAGAAGA AGCAGAAAAA CCTTTATTAA CAAAATCTGC TATTTTGAAG 
ATACTTGCAG AAGCCGTTCG CTCATATAGT GCAGTCGCAA GTTTAATTAC AGAGCATGCA 
TATCGAGCCG GACAAAGTGA AATGGTTCCT GAATACACAA CAGCTCTTGC TTTTCTTTTA 
GATAAGCTTT TACCAATGTC ACCAGAAAAT TCAAGTGACA AACAATGCAG CAGTATGGCG 
AGAATGTTAA TAGCTGCTAT TGCTTCCTGT AATCATTCTC CGGATGTTCA AACAACTTTG 
GTGACTGAAG TAAAAGCAGC TTTAACAAGA ACTTTAGCTC TTCCTGAAAG TTCTGAGAAA 
CATGCACAAA TTCAATTATT AGTTGGATTG ATCTCTACTA TGATTGATAG TTGTCCACCA 
ACTTCTTACA ATCAATTGAG GACTTCCTTG AAGATGCATC AATACAATAA TGTTAACTAT 
ATTGTAAGAA TCATGTTGAG AAAAGGAATA ATTACAGATT TAGCAAAAAT TCCACATAGT 
CTGGATTTAT CAAGTCCAAA TATGGCTGCT ACCATAAATG CTGCTTTAAA ACCATTGGAA 
ACGCTTTCGC GAATTATAAA TCAACCAATG CCAGGTGCTA TTAATAACAA ATTTACAAAG 
CCAAAAAGTA GAACAGTTCA AGAGGAACCT ATTGGAGAAC AAACAGGAAC TACAACATCA 
GAAGCGACAC ATGCTGTTGG CGAAGAAACG AACGAAGACG CAGAGAATAC AGAACACGAT 
ATATCTGTTA CTGCGGAAAG TCTTGAACCT ACTTCAGAAA GTCAAGTACA TGAAGATGAA 
GCTGCTTTAG AAGACATTAT GGATCAACTG CTTGAAAGGG ACGGGCCGGG CGTGACGGAA 
GATCAATCTT CTCGACCACA TAGACCAATG GACATTGATG AAGAAAGCCT TGCAATAAGA 
GATGGAGAAG CTGATTTTGA TCCAACACGA GATGCTACGG AGGATTTAAT GAGCTCTGAT 
TCGGATTCTG ATAGCAATCC ATCTGATGAT AATGAAGATG AAGTGCAAGA TGATGAAGAT 
GAAGAAGAAG AAGAAGAGGA TGATGGAGAA GAAAATGAAG AAGAAGAGGA AGAAGAAGAA 
GAAGGATCCT CAAATTACGA AGAAGACGGA ATTGAATTCT ATGAAGATGT AGGAGAAAGT 
GGTGTATTCA GAATGATTCC ATCAACAGAT CGTGATGGTA GTAGTGTCGT AATGATTCGA 
CATAATATAG ATAATCAAGA CAATGTGAAT TCTTCAACTA CAGTTACACC ACGATCTAGT 
CTTCCATGGA GTACAACATT TCCTCTTCCT TTGGGACTAT TTGAAAATCC TCCATCAATT 
TCTGAAAGTA GTGGTATTAA TTCCCATTCG TTGCTAATAC CACAGGGAGG TTTAGATGCT 
ACTAATACAA GAGGTCAGAG AGCTGTACGA CCGCGAAGAT ATCAATATCT TCAATTACCA 
AATCCACGAA ATCCAAATCC ACCTGTAATA TTGCAAAGAC TTTTAGGTCC AGCTGCACAA 
GCAATTCCAT TATCTGCCAG TCAGGTTTTG GCTTCCAGTT TTCGAGATAC ACGCGTTGTA 
GTAATGGATA ATGGTGTTGG AATATTAACA AATCAAGAAG AAGAACAAAT AGATTTTGTG 
GATCAATCTG GCTATCTTTT CGGATCTAGT CTTGCAACCA CAGTAAATAA TACTCCTACT 
GCTTTACATT GGTGGATTGA AGAAAGCAAA CTATTAGATG GAGATTCCCA AACGGATTGC 
TGTGTTGGTG TTGTACATAA ACTAATTCCT GGATTGGAAA AACGTAGAAA TGCAGAATTG 
GGAGAACGAC GTGAAAGACG TAAAATGCAA TACGCTTCTG AGAAAAAAAA TGAAAAAGAT 
AACAAAGATG GAAAAGATCA TTTAAATAAT ACAGAATCTG GTTCTTCTAC TATAATATCT 
ATGGATGAAC AAATATCAAC TGCATCAACT TCACAAAGAG AAGAAACGAA TAATTTTGTT 
CCTAGATTTG AAACTTGCGT GGAAACAACT CGATTAGAAC GCACACTTGA CGAAGATATA 
ATTGATGTAA CTGGTGAGAT GGATGCAACC ATTCAAGAAG ATGAAGAACG ACCACCACCT 
CCCCCACCAG AAGAACAACC AACAGAACCT AGAGATCCTA GAATAAGCAA TCGAAATCCA 
ACTTTGGAAC TTGCTGAAAG TATAGTGGAT TCTGTACTTG GACCTTGCGC GTCTGAAGCA 
AGCAGATTAA GACAATCTGA ACGTACCGTT GAAGGATCTA ATACTAATGA AACATCTAGT 
CGACCCAATA CGTCCATAGT TGTTGATTTC AGTCAAGAAC CTAATGAAGA TCTATTAAGA 
GAAAGTGATT CATCTGAATG GATAAGAATT CTTACGGATG ACAGTCAATC AAATGTTGAA 
CGAAATGCGA ATATTCTCAA TCAAGTACCT ACCACTTCCG CATCAGAAAA TATTAATCCA 
GAAATATCAG AACAACAACA GCAACAGCAG CAGCAACAAC AACCATCTAT ACAACAGCAG 
CAACAACAAG AGCCACAACA ATCACAAGAA CAGTCGCAAC GACAACAACA AAACCAACAA 
TCTCAAGAAC AACAATTACC GGATGGTGTA GATCCTTCTT TCTTAGCTGC ATTACCCGAA 
GATATGCGTG ACGAAGTTAT TGCTGAGCAA CTAAGACTTC AACGAATTCG GCAAAGAGCA 
CAGGCAACTG TAGTCGAAGA ATTAACAGGA CCGGTTGAAG TTAATCCTGA ATTTTTAGCT 
GCTTTACCTC CAGCAATACA AGAAGAAGTT CTTGCGCAAC AACGCTTGGA ACAACAACGT 
CATGCAGCCG CAAGTGCCAA TCCAGAAGAC CCAGTTGACG CAGCTGCATT CTTCCAAAAT 
TTACAACCTT CTCTTCGACA AGCGATTTTA ACTGATATGG AAGAATCGCA AATATCCGTT 
CTTCCACCAG ACTTAGCTCA AGAAGCTCAA AATCTTCGAA GAGAATGGGA AGCTCGAAAT 
AGACATATAA TGCAAGATCG ATTTCTTAAT CATGTTAGTC AAGGAAATGC CGCCTTATCT 
AGTATATTGC GAAATTCTGG AAGAGGTAGA GGTGGTGGAA CGCGTTATGC AATTCATACA 
GTAGCACAAA GAGGATGGCA ACCATGGTCA AATGGTGATC CTAGTATAAC GCATCATGGA 
GCGGGTCTAC GACTCAGAGG CAGACAATTA TTGGATCATG AGTCGATGGC TTGCGTATTG 
GTATTACTGT TTGTTGATGA ACCAAAAATT AATACATTAA GGCTTCATAG AGTTATCAGG 
AATCTTTGCT ATCACGGTAG CACAAGGGAA TGGGTAGTTA GAGCTTTACT AAGTATCATG 
GAAAGAAGTA ATGATGAAAA TAAAGATATA GCCATGGACT TTGGTGGAAA AATCAGAAGA 
AAAAGCTTAG TGCCCATGAT GCATCACGAT TATTGTGCTC CTAAAATTTT TGATCAAAGA 
AGCAATGCAC AGTCATGGTT AAACATCAGT ATTGATGCTG CGCTTGGATG TAGAGCAAAT 
GTTTTCCATA TTATTAGACA TGGAGGCAAA AAATCAGAAA GACAAGGCAG TAGTATTGCC 
ATCCATCCTC AAGCAGCTCC TACAGTATGC AGGCATACAT TAGAATTATT AATATCATTG 
GCTAAAGGCT TTCATAATAT CACAACTTTT GAGTCATCCG CTTTCGGTCA ATTAATTTCA 
ATGCTAAACT GGTCGGTTAT TAAACGTAGC AGTCAATTAA CGGACAAAGT GTTGAAATTA 
TTGTCTTTAA TTTCAATCGG ATTAACGGAA GTGAATCCGT ATCGTAGACA AGAAGGAAAT 
AAGAATAAAA GAACAGATGT GAATAAAGAA CAAAGTATTG CTGCACCAGA AGAACATCTT 
AAATTGGCTG TGCAAGTATT GACAAGTAAG AGTTGCTCTG AAGAAGGACT TGAAGATGCA 
ACAGCTTTGT TGCTTAATTT ATCTCATTGT CCTGATCCTA CTAGACAATT GATATTAAAA 
TTACTCTTAG AAGGTGCCAT GGAATTGGCT AATGTTGTTT GTGAACACAT TAAAGATTTA 
TTAATGGAGT TAAAAGTTTT GAATCGTGAA CTTAGACGAA ATTCTATCGA AGAGCAACCG 
TCAACTAGTA CTGGAGGTTC TCGTTGGGTT CTTTTAGATA GGTTTACCAA CGAGAGTGTA 
GTTATTACTG CGCCGAGTAA AGTTAAAGCT GGCAGCGATC TTCAATTACC ATCGATGGCT 
GCTCTTGTTA GCAAAACTTC TAGTCAGGCA TTTTTCTTAA GAATACTTAA AGTGATTGTA 
CAAATAAGGG AAGCTGTACG TTTAGCAAAT AATAAAAAAA CATCCACTCA AAGTTCCGAA 
AGTTCTGTAG ATACTGAAAA TACTAATCAA GCTTCAGAAT CAGGTAATTT GAAGCTTTAT 
ATTTTTATAA ACATAGCTTA ATTTTCACAA TTAAATAATA TTTGTATACA ATTTATAGGT 
GAAGATAAGC TTCCATTATT AAGTGAAAGT TTAGTATTAG ATCATCTATG GGAAACACTT 
AGTGCTTGTT TATTGGAATT GGAATATACT CCAGATCAAC ATGCAGTTTT AGTATTGCAA 
CCTGCAGTAG AAGCATTTTT CCTAGTACAT TCATCATCGA GTACTTCTCG TGATCGTCAT 
CTAAGTACAA GTACGGAAAA TCGCGAAGTA GTTCCTGATC TAACACCTGT TTCACCAATT 
TATTCAGATA ATGAAGGAAC GTCCCAATCA GAAGCTACTA TTAATACTTC TTGGGATATA 
CAACCTACGC CACAGAAGAC ATTACCACCG GACCAACTCA AGTTCCTTAA ATTTGCAGAA 
ACGCACAGAA CCGTTTTAAA TCAAATTCTT CGTCAAACAA CTACGCATTT AGCAGATGGA 
CCATTCTCTG TGTTAGTGGA TCACACAAGA GTTCTTGATT TTGACGTAAA AAGACGATAT 
TTCCGAACAG AATTAGAACG AATGGATGAA GGTATTCGAA GGGAAGAATT GGCAGTACAT 
GTTCGTAGGA ATAATGTATT TGAAGATTCA TTTCGAGAAC TTCATAGGCG TAATGCTGAT 
GAATGGAAAA ATCGTTTTTA TATTGTTTTT GAAGGTGAAG AAGGGCAAGA TGCTGGAGGA 
TTACTTAGAG AGTGGTACGT TATTATTTCC AGAGAAATTT TTAATCCTAT GTATGCATTG 
TTTACTGTAA GTCCTGGCGA TCGAGTTACA TACATGATAA ACTCCTCTTC ACATTGTAAT 
CCTAATCACT TATGTTATTA TAAATTTGTC GGTCGAGTTA TAGCTAAAGC AATTTATGAC 
AACAAATTAT TAGAATGTTA CTTCACGCGT AGTTTTTATA AACACATTTT GGGAATACTT 
GTGAAACATA CAGATATGGA AAGTGAGGAT TATAGTTTTT ACAAAGGACT TGTATATCTC 
ACAGAGCATA ATATTGCTGA TCTTGGATAC GAATTAACTT TCTCTACAGA GGTAAACGAA 
TTTGGTGTAA ATGATGTACG TGACTTAATA CCAAATGGAC GTAATATCAT TGTTACTGAG 
GAAACAAAAT TAGAATATAT TCGGCTCGTA TGTCAAATGA AAATGACTGG AGCAATTCGT 
AAACAATTAA ATGCGTTCCT AGAAGGTTTT TATGATATTA TTCCCAAACG ACTGATCTCT 
ATATTCAATG AACAAGAACT TGAATTGTTA ATTAGTGGAT TACCCAATGT AGATATTGAG 
GATCTTAAAG CTAATACTGA ATATCATAAA TATAATGNNN NNNATATTTA GATTCAATGG 
TTCTGGAGAG CATTACGAGG TTTCGATCAA GCAGATAGGG CTAAATTTTT GCAATTTGTT 
ACTGGAACTT CAAAAGTGCC ACTTCAAGGT TTCGCTGCTT TAGAAGGAAT GAATGGTATA 
CAAAAGTTCC AAATTCATCG TGAAGATAGA TCGACAGATA GACTTCCGTC AGCACATACA 
TGTTTCAATC AGCTAGATCT ACCAGTTTAT GAAACATACG ATAAGCTTCG CACGAATTTA 
TTAAAAGCGA TCCACGAATG CAGTGAAGGA TTCGGTTTCG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC411961 ( XM_026442186.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_026297971.1 Apis mellifera E3 ubiquitin-protein ligase HUWE1 (LOC411961), transcript variant X1, mRNA.

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CloneID	OHd32885
Clone ID Related Accession (Same CDS sequence)	XM_026442186.1
Accession Version	XM_026442186.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1537200000000
Organism	Apis mellifera(honey bee)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037645.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATTG ATCGCAATAG ATTGAGAAAA TATACATCAG AAGCGCCTGC AAAATGTCAG 
GCGCTTATAA ACAAGTTGCG CTCATGCTCC CATGCAGAAT TGCTAGAGGA GCTAAAGCAA 
ATAGATGCAT GGACTTTTGG TAAATGTGAA TTATTTCATT GGGTCGATGT ATTGGACCTT 
AGTGACAGCA TTTTGGAAGA AGCTGCTGCT AAAAGCCCAG ATAATCCATG GGAATTGGCT 
TGTGATCTCC CTCAAAATAG AGAGGCAAAA GAACTTCTCC TTTGGGTTCT ACATCTTACA 
ACATTATTGA TTGAACATTC ATATTCGCGA CATTTATACA GCAGTATGGA ACATCTAGTG 
ACTTTATTAT CAAGCTGTGA CATGCATGTT GTCCTTGGTG TATTAAATAT TCTCTACATG 
TTCAGTAAAC GTAGCAATTT CATTGCACGT TTAAATAGTG ACAAGAGACA AGCTTTATTA 
AGTAGACTTA CTCTTTTAGC AGAGAGTTGG GGAGGTAAAG AAAATGGATT TGGTTTAGCA 
GAATGTTGTA AAGAATATCC AGATAAGCCA CTTCCAGCAA GTGCCACAAC ATTGCATTTC 
GAATTTTATG CAGAAAATTC AACGACAGAT TCAAATGCCA CATCAAACAT TGGCACAAAA 
AAATCTGGAC AGACGAATTT TGTAACATGT ATACATATAG AAAATGTAGA TAAACTGGGT 
AAAACACCAG CACAAATTAT GAATGATTTG TTAAAAGTCA ATAACGTACC CCAAGATCGA 
CAGATTGCGT TATTTACACA TATAAGATTA GCACACAGTT TCTCTGACTA TAGAAGACGA 
TTACAATGTG TGCACGCTCG GCTACAGGCA TTATCAATAC TTGTATATAA TAGTGAACTA 
GAAGAAAATG CACATAGTTT GTTGCATCCT GGACTTTTAG AAGAATTAGT TGAATTACTG 
GAACTTCCAC ATGCACATCT TGTTGAATTA CGTGCTGCTG CTTTAAGAAC TCTTACAAGT 
ATCATTCATT TAGATCGCAA TCCACATTTT CCAAAAAAAC CTAGTTCAAG ATTAAATATG 
ATAATCGATG TCACTGGAGC CAATTCATAT CATGGATTTT TTCCTGTATT AATTCGAACT 
TGCATTTCAA CATTAATTTC TCCACAACCT GAAGGACAAC CATTTCCTTT GTCACTTGCA 
ACAGCTTTAT TTAGCTTTCT TTACCATCTT TCAAGTTATG AAGCAGGATG TGAAGCACTT 
GTTAGTTGTG GGATGATGGA AAGTTTGTTA AAAATCATGA ATTGGCCTGG TAGCGAGTTT 
GAACATTTGA CGTTTGTAAC AAGGGCAGTT CGTGTTATAG ACTTAATAAC AAATATAGAT 
ATGCAAGGAT TTCAAGCTCA TGGTGGTTTA GCCAGTTTTA TTAATCGTCT TGATATGGAA 
GTAAATGTTT GTAGAAAAGA ACAACCATTT GAAATAGAAC CAATACATTA TCAAGATAAT 
TCTCAGGCCA CTCGTGAAAG ATCAATGGAT CGCGATGAAA GTTCTACGTC TTCACGTGCT 
TTTGATGAGC GTGAAGAATC ATCTAATCCT ATGGAATTTT CGGAAGATGA GAACATGAGT 
TCCAAAACCG ATGATAAAAA TGAACAAATA CCAGATTATT CAGCTGGAAA AACTGGAAAA 
ACTTGTCTAC CACAGCGAGT TACACTTTTA AAAGCGTTAT TAAATTTTAT TAAAAAAATT 
ATTCAAGATC CTACATTTTC TGATAGTATT AGACATGTTA TGGAGGGTAC TTTACCTTCT 
TCCTTGAGAC ATATTATCAG TAATTCAGAA TATTATGGTC CTTCTTTGTT CCTTATTGCA 
ACAGATGCTG TAACAGTATA TGTTTTCCAA GAGCCATCTT TATTATCTTC TTTACAGGAT 
AATGGATTAA CTGATCTTGT ATTGCATGCT TTACTAATTA AAGAAATTCC TGCTAGTAGA 
GAAGTTCTAG GATCATTGCC TAATGTATTT AGTGCTTTAT GTTTAAATCA GCGTGGACTA 
GAATCATTTG TAAAACGACG TCCTTTCGAA CGCTTATTTA AAGTATTATT ATCGCCACTT 
TATCTGCCTG CAATGAATCG ACGTCGTAGC ACAGATCCTT TAGGTGATAC TGCTTCGAAT 
TTGGGTAATG CAATGGATGA ATTAATGCGA CATCAGCCAA GTTTGAAGGT TGATGCAATC 
GCTGCTGTTA TAAAGTTATT AGAAGAACTT TGTGTACTGG GTTGTGATCC TCGTTATATA 
TGCTTGCAAG CTGAAGATAA ATTCGATAAT TCTCAATCAA ATACTACTTC TGGAAATCGT 
CAATCTTCTG GACAATCTGT TGGAGTTGGA GTTGGAGATT CTGGTGTAGG AGGAGGAGGT 
GGTAGTGGAA GTAGTAGCGA CGAAGAAGAA GAAGATGAAG AAGAAGCTAG TACAAGTTCT 
CATCCACAAC GTGAAGAACA ATGTTCATCA TCCACTGCTC AACCAGTTCC ACCACCTCAA 
CCTAGAGAAA AAACTCCAAT AGCTTTAATG GAATATATAC AAAATGTTAC AAAATTCGTT 
GATGCTATTC TCAGTAATAA TTCCACGGAT GATCATTGTA GAGAATTTGT CGCCCAAAAA 
GGTCTCGTAC CATTATTGTC AATTTTAGGA TTACCTAATC TTCCAGTGGA TCATTCAGTT 
GCCCAAACGG CACAAGCTGT AGCTACAGTT TGTAAAAGTA TTTTAAATCT CGCACATGAA 
CCATTAGTTC TTAAAACTGG TCTATCTCAG TTGAATGAAG TTTTGAATTT GCTGAAACCA 
TTATACAATT ATATGGAAGC TCCAGGAGGA TCTGTTTTAT TACATGAACT TGTCAATGCT 
CCAAATCTGG AAACAGCTTT TACCAGTGAT ACGGCAACAC CTTTACTTCA TGCTATGAAT 
GCTGCTTATG GATATATAAT AATGTTTGCA CAAGTGTGCC GTACCAGTCA AAGTGAAATC 
AGAAACTTGG CTATGAATCA TTGGGGTTCT GAACTTGGTT TGACTGTTCT GAAAGGTTTA 
TCAGAATTAT ATACAGCTCT AATCTGGGAA AGTACAGCCT TATTAGCACT TACCACGGAA 
GATTTTATTC CGGCTGGTTG TGATTTTGGA AAAGAAGATA TGGATAAACT TATACGTCCT 
GAATCAACAA ATGAAGGTGA AAGTACTAGT TGGTCTGGTG CTACATCTAC AGATATGGGA 
AGTAATGGGA TGACTACAGC TATGGAAGCT TTAAGCACTG ATCCGCCTCC TATACCTATG 
GAAGTAGACG AAAAACCAAG TGTTAGTACA GGAAGAATAT CTCCAAATTC TCATCGACTT 
ACATATCCTA AACATTTGCT GGGACTTACA TCTAAAATGG GCAGAGCTTT AGCAGAACTC 
TTTGCTATAC TTGTAAAGCT TTGCATGGGC TCTCCAATTA GACAACGCAG AGGACAACAG 
ATGCCTCCAA TACCAGCCTT ACCTTCACAA GCATCTAGAA GTGTAGCAAC TGCTTTAAAT 
TGTGTATTAA TTCGCGGATT ATCATGGGAT AAATTACCAC CATCGACAGT GCCAAAACTT 
CAAGTAACCT TTTTGATGTG CACTTTAAGT TTTGTATCTC CTGCATTATT TGATGAAAAG 
GGGTATCCAT TTCATTTGAT GCTTCAAAAG TTCGTTAAAC TCGGCGGACA AAATGCATTT 
TTCGCTGCTT TTCATTGGGT ATTATCTGGA GGAGGACAGT TTCCGTTATC TGAAGGATTA 
GAGCATCCAA ATTTACCTGA TTGTACTGGA GAATTCTTAG ATGGTTGGTT GATGATTTTA 
GAAAAAATGG TGAATCCTAA AACTCTTCTT GATTCTCCAT ATGTTGTTTC TTCAAAATGT 
ACTGGAATAT ATAAAGTATA TGAAACATTT GATCCTATAA AATATCTTAC GGATATTCAT 
AAGGAAGCTT TCGAGGCAGT GATGCTAATG TGGGGAAAGA AACCTTTAAA AAATTATGGA 
GTTCATATGA CAGAACTTAT TTTATCTATC TTAAGACATA TTTTACGAAG TGAGCAGATC 
ATAAAGGAAC ATGCTGAAAA AGAAGAAAAT AATGAAGGAG GTATTAGTGG TAGTACTAGT 
GGAACTAGTG GAATCGGAAG AGGTGGAACA ATATCCGATC GCAGAGAAGA ACCAGAAGCA 
GATGTAAATC CAGAACATCT AAGACAGTTG ATGGATATGG GTTTCAGTAG AGCTCATTGC 
ATAGAAGCTT TGCTTCATAC ATTGACAGTT GAACAAGCTA CTGATTATTT GTTAACTAAT 
CCTGCTACTC TACGTCGATC AGATGTGCCA GCAGGTGATG TAAGCGGACA AGATTTAATA 
ATGGATTTAG AATTGGTAGA TGATGATCAA ATAATGCATG CCATAGCGAT GTCGCTTGGA 
GAAACGGCGG AAACGCGAAA TGTTTTCAGC ACAGAAGAAT CCAGTTCACG TGAGGCTACT 
ACCATAACTG AAAGCATAGA TAAATTTACA CAAAATGCTT TAAGTCAATG TTTAAATTTA 
TTGGATCTCA TGCCTGATAC TGTATACAGA ATTTGCGATT TATTAGTTGC CATCGCTAAA 
AGAAACGGAG ATGAATGGCG TGATAATATG TTGCGACAAC TTATGAAAGA AATTGGAGGT 
TTGGTAGATT ATTTAATCGA AGTTGCTGAA AGTCAAGACC CGAATGCTGG AGTAAAATTA 
GTAGAATGTA AAGAAGCTAA TAAAGTTACT GGACGTATTT ATCTGTATAC AATGTTCTTT 
GAAGGAACAT TCCAAGAAAT GCGAGTGCCC TGTGCTCAAA TTGTAGAAGA ATATGCTATT 
TTAAATAAAC TTGTACGTTT ATTAGTCGTA AGTGAGGGTC CGTTAACTGC AAGTAAAAGT 
AAAATTGTTA AGGAAGGTAA TAAGGATACA TCATCTACGA AAACTAGAAA ATGGCTTGCA 
CCTTTGTTAT TGCTGATAGA ACGTTTAGAA AAAGTAGCGG TATTGACAAA GAGAAAAGAA 
TTAATGTATA AAGTAACAAC CAGGATGTGG AAATGGTTCG ATCTCAGCAC AGGAAAATGG 
AATTTATATT CTCCAGTAAA TAATCGAATA ATTAATGATG CGTATTGGGC TGGCGAGTCT 
AGTACGAGAG TAACATGTAA CAGGCGACGA TATGTAATTA ATTTTTCATG CATGACTCAA 
GTTAATGAAG AAACTGGTAA TAGAAGACCT ATTACGATGG GCTTTAAATT GCATACCGGA 
AATGAAGATG GTGGATCAGG TTCTGATACA AATATGGACA CTGACGAAAA TCGAATAGAA 
GATAAACGGA ATACAGTTTT GCAAGGGTTA GATCCTAGCA TGGCTCCAAG TATCATACGC 
GCTTGTGTAA GATTGATGGC TATTCCAGTA GATCGAGATG CTTTACATGC AGCTATGAAA 
GTTTGTTTGA GACTTACAAG AGATTATGAA AATGCCGAAA TATTTGTTCG TGAGGGTGGT 
GTGAAACTTC TTCTAGAAAT GTCTCAAGCA AGTAGTTTCA TTGGATGCAT AACTTTAAGT 
ACTCTTTTAA TAAGGCATGC TTTGGAAGAA CCATGTACTC TTCGATTAGC CATGGAAAAA 
GTAATTCGTA GTCGTACTCA AGGCAATATT CCTCCAGCTT GCAAAGAAAT CTTATTTATG 
ACACGTCAGA TTAATAGCGC TGTATGTCGC AATCCGGAAG TTTATAGAGA AGTTTGTGAA 
AACATTTTAA GAGTAGATAT TCGTCTATTA GGAAAAGAGG ATGATGATAA TAGATTAATT 
GTCAAGGCAC TTCCACCAAG TCACTCATCA GTAATGCCTA TGGTAGAGAA AGCTTCAATA 
TCTGTTGTAA AAGATCTTCT GAATGCTCTT ATAAAGCCTA TGCCAGTTGT CTCTGATGAA 
AAGTCTTCTA CACCAACAAG GAGTCCAGAA AAGAAGACAT CTTATTCTAA TGTTGGAAGT 
TCATCTTCTC GACGAGATGT TCTTAGAAAT AATGCAACAA CATCAAATGA TACTCTTGAT 
GATGAGGATC AAGTTTCTCA AATAATACCA ATGAAAATTT ATCCAGATAA TAGCAATAAC 
AGTAAAGTGG AACCAGAAGA AGCAGAAAAA CCTTTATTAA CAAAATCTGC TATTTTGAAG 
ATACTTGCAG AAGCCGTTCG CTCATATAGT GCAGTCGCAA GTTTAATTAC AGAGCATGCA 
TATCGAGCCG GACAAAGTGA AATGGTTCCT GAATACACAA CAGCTCTTGC TTTTCTTTTA 
GATAAGCTTT TACCAATGTC ACCAGAAAAT TCAAGTGACA AACAATGCAG CAGTATGGCG 
AGAATGTTAA TAGCTGCTAT TGCTTCCTGT AATCATTCTC CGGATGTTCA AACAACTTTG 
GTGACTGAAG TAAAAGCAGC TTTAACAAGA ACTTTAGCTC TTCCTGAAAG TTCTGAGAAA 
CATGCACAAA TTCAATTATT AGTTGGATTG ATCTCTACTA TGATTGATAG TTGTCCACCA 
ACTTCTTACA ATCAATTGAG GACTTCCTTG AAGATGCATC AATACAATAA TGTTAACTAT 
ATTGTAAGAA TCATGTTGAG AAAAGGAATA ATTACAGATT TAGCAAAAAT TCCACATAGT 
CTGGATTTAT CAAGTCCAAA TATGGCTGCT ACCATAAATG CTGCTTTAAA ACCATTGGAA 
ACGCTTTCGC GAATTATAAA TCAACCAATG CCAGGTGCTA TTAATAACAA ATTTACAAAG 
CCAAAAAGTA GAACAGTTCA AGAGGAACCT ATTGGAGAAC AAACAGGAAC TACAACATCA 
GAAGCGACAC ATGCTGTTGG CGAAGAAACG AACGAAGACG CAGAGAATAC AGAACACGAT 
ATATCTGTTA CTGCGGAAAG TCTTGAACCT ACTTCAGAAA GTCAAGTACA TGAAGATGAA 
GCTGCTTTAG AAGACATTAT GGATCAACTG CTTGAAAGTG TACTATTTTC CAGGGACGGG 
CCGGGCGTGA CGGAAGATCA ATCTTCTCGA CCACATAGAC CAATGGACAT TGATGAAGAA 
AGCCTTGCAA TAAGAGATGG AGAAGCTGAT TTTGATCCAA CACGAGATGC TACGGAGGAT 
TTAATGAGCT CTGATTCGGA TTCTGATAGC AATCCATCTG ATGATAATGA AGATGAAGTG 
CAAGATGATG AAGATGAAGA AGAAGAAGAA GAGGATGATG GAGAAGAAAA TGAAGAAGAA 
GAGGAAGAAG AAGAAGAAGG ATCCTCAAAT TACGAAGAAG ACGGAATTGA ATTCTATGAA 
GATGTAGGAG AAAGTGGTGT ATTCAGAATG ATTCCATCAA CAGATCGTGA TGGTAGTAGT 
GTCGTAATGA TTCGACATAA TATAGATAAT CAAGACAATG TGAATTCTTC AACTACAGTT 
ACACCACGAT CTAGTCTTCC ATGGAGTACA ACATTTCCTC TTCCTTTGGG ACTATTTGAA 
AATCCTCCAT CAATTTCTGA AAGTAGTGGT ATTAATTCCC ATTCGTTGCT AATACCACAG 
GGAGGTTTAG ATGCTACTAA TACAAGAGGT CAGAGAGCTG TACGACCGCG AAGATATCAA 
TATCTTCAAT TACCAAATCC ACGAAATCCA AATCCACCTG TAATATTGCA AAGACTTTTA 
GGTCCAGCTG CACAAGCAAT TCCATTATCT GCCAGTCAGG TTTTGGCTTC CAGTTTTCGA 
GATACACGCG TTGTAGTAAT GGATAATGGT GTTGGAATAT TAACAAATCA AGAAGAAGAA 
CAAATAGATT TTGTGGATCA ATCTGGCTAT CTTTTCGGAT CTAGTCTTGC AACCACAGTA 
AATAATACTC CTACTGCTTT ACATTGGTGG ATTGAAGAAA GCAAACTATT AGATGGAGAT 
TCCCAAACGG ATTGCTGTGT TGGTGTTGTA CATAAACTAA TTCCTGGATT GGAAAAACGT 
AGAAATGCAG AATTGGGAGA ACGACGTGAA AGACGTAAAA TGCAATACGC TTCTGAGAAA 
AAAAATGAAA AAGATAACAA AGATGGAAAA GATCATTTAA ATAATACAGA ATCTGGTTCT 
TCTACTATAA TATCTATGGA TGAACAAATA TCAACTGCAT CAACTTCACA AAGAGAAGAA 
ACGAATAATT TTGTTCCTAG ATTTGAAACT TGCGTGGAAA CAACTCGATT AGAACGCACA 
CTTGACGAAG ATATAATTGA TGTAACTGGT GAGATGGATG CAACCATTCA AGAAGATGAA 
GAACGACCAC CACCTCCCCC ACCAGAAGAA CAACCAACAG AACCTAGAGA TCCTAGAATA 
AGCAATCGAA ATCCAACTTT GGAACTTGCT GAAAGTATAG TGGATTCTGT ACTTGGACCT 
TGCGCGTCTG AAGCAAGCAG ATTAAGACAA TCTGAACGTA CCGTTGAAGG ATCTAATACT 
AATGAAACAT CTAGTCGACC CAATACGTCC ATAGTTGTTG ATTTCAGTCA AGAACCTAAT 
GAAGATCTAT TAAGAGAAAG TGATTCATCT GAATGGATAA GAATTCTTAC GGATGACAGT 
CAATCAAATG TTGAACGAAA TGCGAATATT CTCAATCAAG TACCTACCAC TTCCGCATCA 
GAAAATATTA ATCCAGAAAT ATCAGAACAA CAACAGCAAC AGCAGCAGCA ACAACAACCA 
TCTATACAAC AGCAGCAACA ACAAGAGCCA CAACAATCAC AAGAACAGTC GCAACGACAA 
CAACAAAACC AACAATCTCA AGAACAACAA TTACCGGATG GTGTAGATCC TTCTTTCTTA 
GCTGCATTAC CCGAAGATAT GCGTGACGAA GTTATTGCTG AGCAACTAAG ACTTCAACGA 
ATTCGGCAAA GAGCACAGGC AACTGTAGTC GAAGAATTAA CAGGACCGGT TGAAGTTAAT 
CCTGAATTTT TAGCTGCTTT ACCTCCAGCA ATACAAGAAG AAGTTCTTGC GCAACAACGC 
TTGGAACAAC AACGTCATGC AGCCGCAAGT GCCAATCCAG AAGACCCAGT TGACGCAGCT 
GCATTCTTCC AAAATTTACA ACCTTCTCTT CGACAAGCGA TTTTAACTGA TATGGAAGAA 
TCGCAAATAT CCGTTCTTCC ACCAGACTTA GCTCAAGAAG CTCAAAATCT TCGAAGAGAA 
TGGGAAGCTC GAAATAGACA TATAATGCAA GATCGATTTC TTAATCATGT TAGTCAAGGA 
AATGCCGCCT TATCTAGTAT ATTGCGAAAT TCTGGAAGAG GTAGAGGTGG TGGAACGCGT 
TATGCAATTC ATACAGTAGC ACAAAGAGGA TGGCAACCAT GGTCAAATGG TGATCCTAGT 
ATAACGCATC ATGGAGCGGG TCTACGACTC AGAGGCAGAC AATTATTGGA TCATGAGTCG 
ATGGCTTGCG TATTGGTATT ACTGTTTGTT GATGAACCAA AAATTAATAC ATTAAGGCTT 
CATAGAGTTA TCAGGAATCT TTGCTATCAC GGTAGCACAA GGGAATGGGT AGTTAGAGCT 
TTACTAAGTA TCATGGAAAG AAGTAATGAT GAAAATAAAG ATATAGCCAT GGACTTTGGT 
GGAAAAATCA GAAGAAAAAG CTTAGTGCCC ATGATGCATC ACGATTATTG TGCTCCTAAA 
ATTTTTGATC AAAGAAGCAA TGCACAGTCA TGGTTAAACA TCAGTATTGA TGCTGCGCTT 
GGATGTAGAG CAAATGTTTT CCATATTATT AGACATGGAG GCAAAAAATC AGAAAGACAA 
GGCAGTAGTA TTGCCATCCA TCCTCAAGCA GCTCCTACAG TATGCAGGCA TACATTAGAA 
TTATTAATAT CATTGGCTAA AGGCTTTCCT GGTTACTTTG TACCAATCAA GTCTAAAGAA 
TCAGATGAAA AAGAAAGTAA CAAAGAAAAA GATTCAAATA ACAAAGAAGA AGTCGGTTCG 
AAAGGTAAAT CGACATCGAA ACCAGGAAAA AGTGATCAAC CTGATTTTTG GGATATGTTG 
TTGAAACTTG ATTCATGTGC TTCGAAAAAA GGAAAATCAG TTGCTAGATC GCACTCAAAT 
TCAAATTTAG GAAATGATAC GGAACATAAT ATCACAACTT TTGAGTCATC CGCTTTCGGT 
CAATTAATTT CAATGCTAAA CTGGTCGGTT ATTAAACGTA GCAGTCAATT AACGGACAAA 
GTGTTGAAAT TATTGTCTTT AATTTCAATC GGATTAACGG AAGTGAATCC GTATCGTAGA 
CAAGAAGGAA ATAAGAATAA AAGAACAGAT GTGAATAAAG AACAAAGTAT TGCTGCACCA 
GAAGAACATC TTAAATTGGC TGTGCAAGTA TTGACAAGTA AGAGTTGCTC TGAAGAAGGA 
CTTGAAGATG CAACAGCTTT GTTGCTTAAT TTATCTCATT GTCCTGATCC TACTAGACAA 
TTGATATTAA AATTACTCTT AGAAGGTGCC ATGGAATTGG CTAATGTTGT TTGTGAACAC 
ATTAAAGATT TATTAATGGA GTTAAAAGTT TTGAATCGTG AACTTAGACG AAATTCTATC 
GAAGAGCAAC CGTCAACTAG TACTGGAGGT TCTCGTTGGG TTCTTTTAGA TAGGTTTACC 
AACGAGAGTG TAGTTATTAC TGCGCCGAGT AAAGTTAAAG CTGGCAGCGA TCTTCAATTA 
CCATCGATGG CTGCTCTTGT TAGCAAAACT TCTAGTCAGG CATTTTTCTT AAGAATACTT 
AAAGTGATTG TACAAATAAG GGAAGCTGTA CGTTTAGCAA ATAATAAAAA AACATCCACT 
CAAAGTTCCG AAAGTTCTGT AGATACTGAA AATACTAATC AAGCTTCAGA ATCAGGTGAA 
GATAAGCTTC CATTATTAAG TGAAAGTTTA GTATTAGATC ATCTATGGGA AACACTTAGT 
GCTTGTTTAT TGGAATTGGA ATATACTCCA GATCAACATG CAGTTTTAGT ATTGCAACCT 
GCAGTAGAAG CATTTTTCCT AGTACATTCA TCATCGAGTA CTTCTCGTGA TCGTCATCTA 
AGTACAAGTA CGGAAAATCG CGAAGTAGTT CCTGATCTAA CACCTGTTTC ACCAATTTAT 
TCAGATAATG AAGGAACGTC CCAATCAGAA GCTACTATTA ATACTTCTTG GGATATACAA 
CCTACGCCAC AGAAGACATT ACCACCGGAC CAACTCAAGT TCCTTAAATT TGCAGAAACG 
CACAGAACCG TTTTAAATCA AATTCTTCGT CAAACAACTA CGCATTTAGC AGATGGACCA 
TTCTCTGTGT TAGTGGATCA CACAAGAGTT CTTGATTTTG ACGTAAAAAG ACGATATTTC 
CGAACAGAAT TAGAACGAAT GGATGAAGGT ATTCGAAGGG AAGAATTGGC AGTACATGTT 
CGTAGGAATA ATGTATTTGA AGATTCATTT CGAGAACTTC ATAGGCGTAA TGCTGATGAA 
TGGAAAAATC GTTTTTATAT TGTTTTTGAA GGTGAAGAAG GGCAAGATGC TGGAGGATTA 
CTTAGAGAGT GGTACGTTAT TATTTCCAGA GAAATTTTTA ATCCTATGTA TGCATTGTTT 
ACTGTAAGTC CTGGCGATCG AGTTACATAC ATGATAAACT CCTCTTCACA TTGTAATCCT 
AATCACTTAT GTTATTATAA ATTTGTCGGT CGAGTTATAG CTAAAGCAAT TTATGACAAC 
AAATTATTAG AATGTTACTT CACGCGTAGT TTTTATAAAC ACATTTTGGG AATACTTGTG 
AAACATACAG ATATGGAAAG TGAGGATTAT AGTTTTTACA AAGGACTTGT ATATCTCACA 
GAGCATAATA TTGCTGATCT TGGATACGAA TTAACTTTCT CTACAGAGGT AAACGAATTT 
GGTGTAAATG ATGTACGTGA CTTAATACCA AATGGACGTA ATATCATTGT TACTGAGGAA 
ACAAAATTAG AATATATTCG GCTCGTATGT CAAATGAAAA TGACTGGAGC AATTCGTAAA 
CAATTAAATG CGTTCCTAGA AGGTTTTTAT GATATTATTC CCAAACGACT GATCTCTATA 
TTCAATGAAC AAGAACTTGA ATTGTTAATT AGTGGATTAC CCAATGTAGA TATTGAGGAT 
CTTAAAGCTA ATACTGAATA TCATAAATAT AATGCAAATT CTTTACAAAT TCAATGGTTC 
TGGAGAGCAT TACGAGGTTT CGATCAAGCA GATAGGGCTA AATTTTTGCA ATTTGTTACT 
GGAACTTCAA AAGTGCCACT TCAAGGTTTC GCTGCTTTAG AAGGAATGAA TGGTATACAA 
AAGTTCCAAA TTCATCGTGA AGATAGATCG ACAGATAGAC TTCCGTCAGC ACATACATGT 
TTCAATCAGC TAGATCTACC AGTTTATGAA ACATACGATA AGCTTCGCAC GAATTTATTA 
AAAGCGATCC ACGAATGCAG TGAAGGATTC GGTTTCGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026297971.1
CDS	794..13255
Translation	MKIDRNRLRKYTSEAPAKCQALINKLRSCSHAELLEELKQIDAWTFGKCELFHWVDVLDLSDSILEEAAAKSPDNPWELACDLPQNREAKELLLWVLHLTTLLIEHSYSRHLYSSMEHLVTLLSSCDMHVVLGVLNILYMFSKRSNFIARLNSDKRQALLSRLTLLAESWGGKENGFGLAECCKEYPDKPLPASATTLHFEFYAENSTTDSNATSNIGTKKSGQTNFVTCIHIENVDKLGKTPAQIMNDLLKVNNVPQDRQIALFTHIRLAHSFSDYRRRLQCVHARLQALSILVYNSELEENAHSLLHPGLLEELVELLELPHAHLVELRAAALRTLTSIIHLDRNPHFPKKPSSRLNMIIDVTGANSYHGFFPVLIRTCISTLISPQPEGQPFPLSLATALFSFLYHLSSYEAGCEALVSCGMMESLLKIMNWPGSEFEHLTFVTRAVRVIDLITNIDMQGFQAHGGLASFINRLDMEVNVCRKEQPFEIEPIHYQDNSQATRERSMDRDESSTSSRAFDEREESSNPMEFSEDENMSSKTDDKNEQIPDYSAGKTGKTCLPQRVTLLKALLNFIKKIIQDPTFSDSIRHVMEGTLPSSLRHIISNSEYYGPSLFLIATDAVTVYVFQEPSLLSSLQDNGLTDLVLHALLIKEIPASREVLGSLPNVFSALCLNQRGLESFVKRRPFERLFKVLLSPLYLPAMNRRRSTDPLGDTASNLGNAMDELMRHQPSLKVDAIAAVIKLLEELCVLGCDPRYICLQAEDKFDNSQSNTTSGNRQSSGQSVGVGVGDSGVGGGGGSGSSSDEEEEDEEEASTSSHPQREEQCSSSTAQPVPPPQPREKTPIALMEYIQNVTKFVDAILSNNSTDDHCREFVAQKGLVPLLSILGLPNLPVDHSVAQTAQAVATVCKSILNLAHEPLVLKTGLSQLNEVLNLLKPLYNYMEAPGGSVLLHELVNAPNLETAFTSDTATPLLHAMNAAYGYIIMFAQVCRTSQSEIRNLAMNHWGSELGLTVLKGLSELYTALIWESTALLALTTEDFIPAGCDFGKEDMDKLIRPESTNEGESTSWSGATSTDMGSNGMTTAMEALSTDPPPIPMEVDEKPSVSTGRISPNSHRLTYPKHLLGLTSKMGRALAELFAILVKLCMGSPIRQRRGQQMPPIPALPSQASRSVATALNCVLIRGLSWDKLPPSTVPKLQVTFLMCTLSFVSPALFDEKGYPFHLMLQKFVKLGGQNAFFAAFHWVLSGGGQFPLSEGLEHPNLPDCTGEFLDGWLMILEKMVNPKTLLDSPYVVSSKCTGIYKVYETFDPIKYLTDIHKEAFEAVMLMWGKKPLKNYGVHMTELILSILRHILRSEQIIKEHAEKEENNEGGISGSTSGTSGIGRGGTISDRREEPEADVNPEHLRQLMDMGFSRAHCIEALLHTLTVEQATDYLLTNPATLRRSDVPAGDVSGQDLIMDLELVDDDQIMHAIAMSLGETAETRNVFSTEESSSREATTITESIDKFTQNALSQCLNLLDLMPDTVYRICDLLVAIAKRNGDEWRDNMLRQLMKEIGGLVDYLIEVAESQDPNAGVKLVECKEANKVTGRIYLYTMFFEGTFQEMRVPCAQIVEEYAILNKLVRLLVVSEGPLTASKSKIVKEGNKDTSSTKTRKWLAPLLLLIERLEKVAVLTKRKELMYKVTTRMWKWFDLSTGKWNLYSPVNNRIINDAYWAGESSTRVTCNRRRYVINFSCMTQVNEETGNRRPITMGFKLHTGNEDGGSGSDTNMDTDENRIEDKRNTVLQGLDPSMAPSIIRACVRLMAIPVDRDALHAAMKVCLRLTRDYENAEIFVREGGVKLLLEMSQASSFIGCITLSTLLIRHALEEPCTLRLAMEKVIRSRTQGNIPPACKEILFMTRQINSAVCRNPEVYREVCENILRVDIRLLGKEDDDNRLIVKALPPSHSSVMPMVEKASISVVKDLLNALIKPMPVVSDEKSSTPTRSPEKKTSYSNVGSSSSRRDVLRNNATTSNDTLDDEDQVSQIIPMKIYPDNSNNSKVEPEEAEKPLLTKSAILKILAEAVRSYSAVASLITEHAYRAGQSEMVPEYTTALAFLLDKLLPMSPENSSDKQCSSMARMLIAAIASCNHSPDVQTTLVTEVKAALTRTLALPESSEKHAQIQLLVGLISTMIDSCPPTSYNQLRTSLKMHQYNNVNYIVRIMLRKGIITDLAKIPHSLDLSSPNMAATINAALKPLETLSRIINQPMPGAINNKFTKPKSRTVQEEPIGEQTGTTTSEATHAVGEETNEDAENTEHDISVTAESLEPTSESQVHEDEAALEDIMDQLLESVLFSRDGPGVTEDQSSRPHRPMDIDEESLAIRDGEADFDPTRDATEDLMSSDSDSDSNPSDDNEDEVQDDEDEEEEEEDDGEENEEEEEEEEEGSSNYEEDGIEFYEDVGESGVFRMIPSTDRDGSSVVMIRHNIDNQDNVNSSTTVTPRSSLPWSTTFPLPLGLFENPPSISESSGINSHSLLIPQGGLDATNTRGQRAVRPRRYQYLQLPNPRNPNPPVILQRLLGPAAQAIPLSASQVLASSFRDTRVVVMDNGVGILTNQEEEQIDFVDQSGYLFGSSLATTVNNTPTALHWWIEESKLLDGDSQTDCCVGVVHKLIPGLEKRRNAELGERRERRKMQYASEKKNEKDNKDGKDHLNNTESGSSTIISMDEQISTASTSQREETNNFVPRFETCVETTRLERTLDEDIIDVTGEMDATIQEDEERPPPPPPEEQPTEPRDPRISNRNPTLELAESIVDSVLGPCASEASRLRQSERTVEGSNTNETSSRPNTSIVVDFSQEPNEDLLRESDSSEWIRILTDDSQSNVERNANILNQVPTTSASENINPEISEQQQQQQQQQQPSIQQQQQQEPQQSQEQSQRQQQNQQSQEQQLPDGVDPSFLAALPEDMRDEVIAEQLRLQRIRQRAQATVVEELTGPVEVNPEFLAALPPAIQEEVLAQQRLEQQRHAAASANPEDPVDAAAFFQNLQPSLRQAILTDMEESQISVLPPDLAQEAQNLRREWEARNRHIMQDRFLNHVSQGNAALSSILRNSGRGRGGGTRYAIHTVAQRGWQPWSNGDPSITHHGAGLRLRGRQLLDHESMACVLVLLFVDEPKINTLRLHRVIRNLCYHGSTREWVVRALLSIMERSNDENKDIAMDFGGKIRRKSLVPMMHHDYCAPKIFDQRSNAQSWLNISIDAALGCRANVFHIIRHGGKKSERQGSSIAIHPQAAPTVCRHTLELLISLAKGFPGYFVPIKSKESDEKESNKEKDSNNKEEVGSKGKSTSKPGKSDQPDFWDMLLKLDSCASKKGKSVARSHSNSNLGNDTEHNITTFESSAFGQLISMLNWSVIKRSSQLTDKVLKLLSLISIGLTEVNPYRRQEGNKNKRTDVNKEQSIAAPEEHLKLAVQVLTSKSCSEEGLEDATALLLNLSHCPDPTRQLILKLLLEGAMELANVVCEHIKDLLMELKVLNRELRRNSIEEQPSTSTGGSRWVLLDRFTNESVVITAPSKVKAGSDLQLPSMAALVSKTSSQAFFLRILKVIVQIREAVRLANNKKTSTQSSESSVDTENTNQASESGEDKLPLLSESLVLDHLWETLSACLLELEYTPDQHAVLVLQPAVEAFFLVHSSSSTSRDRHLSTSTENREVVPDLTPVSPIYSDNEGTSQSEATINTSWDIQPTPQKTLPPDQLKFLKFAETHRTVLNQILRQTTTHLADGPFSVLVDHTRVLDFDVKRRYFRTELERMDEGIRREELAVHVRRNNVFEDSFRELHRRNADEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFTVSPGDRVTYMINSSSHCNPNHLCYYKFVGRVIAKAIYDNKLLECYFTRSFYKHILGILVKHTDMESEDYSFYKGLVYLTEHNIADLGYELTFSTEVNEFGVNDVRDLIPNGRNIIVTEETKLEYIRLVCQMKMTGAIRKQLNAFLEGFYDIIPKRLISIFNEQELELLISGLPNVDIEDLKANTEYHKYNANSLQIQWFWRALRGFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHREDRSTDRLPSAHTCFNQLDLPVYETYDKLRTNLLKAIHECSEGFGFA

Target ORF information:

RefSeq Version	XM_026442186.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera E3 ubiquitin-protein ligase HUWE1 (LOC411961), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026442186.1

ORF Insert Sequence:

ATGAAAATTG ATCGCAATAG ATTGAGAAAA TATACATCAG AAGCGCCTGC AAAATGTCAG 
GCGCTTATAA ACAAGTTGCG CTCATGCTCC CATGCAGAAT TGCTAGAGGA GCTAAAGCAA 
ATAGATGCAT GGACTTTTGG TAAATGTGAA TTATTTCATT GGGTCGATGT ATTGGACCTT 
AGTGACAGCA TTTTGGAAGA AGCTGCTGCT AAAAGCCCAG ATAATCCATG GGAATTGGCT 
TGTGATCTCC CTCAAAATAG AGAGGCAAAA GAACTTCTCC TTTGGGTTCT ACATCTTACA 
ACATTATTGA TTGAACATTC ATATTCGCGA CATTTATACA GCAGTATGGA ACATCTAGTG 
ACTTTATTAT CAAGCTGTGA CATGCATGTT GTCCTTGGTG TATTAAATAT TCTCTACATG 
TTCAGTAAAC GTAGCAATTT CATTGCACGT TTAAATAGTG ACAAGAGACA AGCTTTATTA 
AGTAGACTTA CTCTTTTAGC AGAGAGTTGG GGAGGTAAAG AAAATGGATT TGGTTTAGCA 
GAATGTTGTA AAGAATATCC AGATAAGCCA CTTCCAGCAA GTGCCACAAC ATTGCATTTC 
GAATTTTATG CAGAAAATTC AACGACAGAT TCAAATGCCA CATCAAACAT TGGCACAAAA 
AAATCTGGAC AGACGAATTT TGTAACATGT ATACATATAG AAAATGTAGA TAAACTGGGT 
AAAACACCAG CACAAATTAT GAATGATTTG TTAAAAGTCA ATAACGTACC CCAAGATCGA 
CAGATTGCGT TATTTACACA TATAAGATTA GCACACAGTT TCTCTGACTA TAGAAGACGA 
TTACAATGTG TGCACGCTCG GCTACAGGCA TTATCAATAC TTGTATATAA TAGTGAACTA 
GAAGAAAATG CACATAGTTT GTTGCATCCT GGACTTTTAG AAGAATTAGT TGAATTACTG 
GAACTTCCAC ATGCACATCT TGTTGAATTA CGTGCTGCTG CTTTAAGAAC TCTTACAAGT 
ATCATTCATT TAGATCGCAA TCCACATTTT CCAAAAAAAC CTAGTTCAAG ATTAAATATG 
ATAATCGATG TCACTGGAGC CAATTCATAT CATGGATTTT TTCCTGTATT AATTCGAACT 
TGCATTTCAA CATTAATTTC TCCACAACCT GAAGGACAAC CATTTCCTTT GTCACTTGCA 
ACAGCTTTAT TTAGCTTTCT TTACCATCTT TCAAGTTATG AAGCAGGATG TGAAGCACTT 
GTTAGTTGTG GGATGATGGA AAGTTTGTTA AAAATCATGA ATTGGCCTGG TAGCGAGTTT 
GAACATTTGA CGTTTGTAAC AAGGGCAGTT CGTGTTATAG ACTTAATAAC AAATATAGAT 
ATGCAAGGAT TTCAAGCTCA TGGTGGTTTA GCCAGTTTTA TTAATCGTCT TGATATGGAA 
GTAAATGTTT GTAGAAAAGA ACAACCATTT GAAATAGAAC CAATACATTA TCAAGATAAT 
TCTCAGGCCA CTCGTGAAAG ATCAATGGAT CGCGATGAAA GTTCTACGTC TTCACGTGCT 
TTTGATGAGC GTGAAGAATC ATCTAATCCT ATGGAATTTT CGGAAGATGA GAACATGAGT 
TCCAAAACCG ATGATAAAAA TGAACAAATA CCAGATTATT CAGCTGGAAA AACTGGAAAA 
ACTTGTCTAC CACAGCGAGT TACACTTTTA AAAGCGTTAT TAAATTTTAT TAAAAAAATT 
ATTCAAGATC CTACATTTTC TGATAGTATT AGACATGTTA TGGAGGGTAC TTTACCTTCT 
TCCTTGAGAC ATATTATCAG TAATTCAGAA TATTATGGTC CTTCTTTGTT CCTTATTGCA 
ACAGATGCTG TAACAGTATA TGTTTTCCAA GAGCCATCTT TATTATCTTC TTTACAGGAT 
AATGGATTAA CTGATCTTGT ATTGCATGCT TTACTAATTA AAGAAATTCC TGCTAGTAGA 
GAAGTTCTAG GATCATTGCC TAATGTATTT AGTGCTTTAT GTTTAAATCA GCGTGGACTA 
GAATCATTTG TAAAACGACG TCCTTTCGAA CGCTTATTTA AAGTATTATT ATCGCCACTT 
TATCTGCCTG CAATGAATCG ACGTCGTAGC ACAGATCCTT TAGGTGATAC TGCTTCGAAT 
TTGGGTAATG CAATGGATGA ATTAATGCGA CATCAGCCAA GTTTGAAGGT TGATGCAATC 
GCTGCTGTTA TAAAGTTATT AGAAGAACTT TGTGTACTGG GTTGTGATCC TCGTTATATA 
TGCTTGCAAG CTGAAGATAA ATTCGATAAT TCTCAATCAA ATACTACTTC TGGAAATCGT 
CAATCTTCTG GACAATCTGT TGGAGTTGGA GTTGGAGATT CTGGTGTAGG AGGAGGAGGT 
GGTAGTGGAA GTAGTAGCGA CGAAGAAGAA GAAGATGAAG AAGAAGCTAG TACAAGTTCT 
CATCCACAAC GTGAAGAACA ATGTTCATCA TCCACTGCTC AACCAGTTCC ACCACCTCAA 
CCTAGAGAAA AAACTCCAAT AGCTTTAATG GAATATATAC AAAATGTTAC AAAATTCGTT 
GATGCTATTC TCAGTAATAA TTCCACGGAT GATCATTGTA GAGAATTTGT CGCCCAAAAA 
GGTCTCGTAC CATTATTGTC AATTTTAGGA TTACCTAATC TTCCAGTGGA TCATTCAGTT 
GCCCAAACGG CACAAGCTGT AGCTACAGTT TGTAAAAGTA TTTTAAATCT CGCACATGAA 
CCATTAGTTC TTAAAACTGG TCTATCTCAG TTGAATGAAG TTTTGAATTT GCTGAAACCA 
TTATACAATT ATATGGAAGC TCCAGGAGGA TCTGTTTTAT TACATGAACT TGTCAATGCT 
CCAAATCTGG AAACAGCTTT TACCAGTGAT ACGGCAACAC CTTTACTTCA TGCTATGAAT 
GCTGCTTATG GATATATAAT AATGTTTGCA CAAGTGTGCC GTACCAGTCA AAGTGAAATC 
AGAAACTTGG CTATGAATCA TTGGGGTTCT GAACTTGGTT TGACTGTTCT GAAAGGTTTA 
TCAGAATTAT ATACAGCTCT AATCTGGGAA AGTACAGCCT TATTAGCACT TACCACGGAA 
GATTTTATTC CGGCTGGTTG TGATTTTGGA AAAGAAGATA TGGATAAACT TATACGTCCT 
GAATCAACAA ATGAAGGTGA AAGTACTAGT TGGTCTGGTG CTACATCTAC AGATATGGGA 
AGTAATGGGA TGACTACAGC TATGGAAGCT TTAAGCACTG ATCCGCCTCC TATACCTATG 
GAAGTAGACG AAAAACCAAG TGTTAGTACA GGAAGAATAT CTCCAAATTC TCATCGACTT 
ACATATCCTA AACATTTGCT GGGACTTACA TCTAAAATGG GCAGAGCTTT AGCAGAACTC 
TTTGCTATAC TTGTAAAGCT TTGCATGGGC TCTCCAATTA GACAACGCAG AGGACAACAG 
ATGCCTCCAA TACCAGCCTT ACCTTCACAA GCATCTAGAA GTGTAGCAAC TGCTTTAAAT 
TGTGTATTAA TTCGCGGATT ATCATGGGAT AAATTACCAC CATCGACAGT GCCAAAACTT 
CAAGTAACCT TTTTGATGTG CACTTTAAGT TTTGTATCTC CTGCATTATT TGATGAAAAG 
GGGTATCCAT TTCATTTGAT GCTTCAAAAG TTCGTTAAAC TCGGCGGACA AAATGCATTT 
TTCGCTGCTT TTCATTGGGT ATTATCTGGA GGAGGACAGT TTCCGTTATC TGAAGGATTA 
GAGCATCCAA ATTTACCTGA TTGTACTGGA GAATTCTTAG ATGGTTGGTT GATGATTTTA 
GAAAAAATGG TGAATCCTAA AACTCTTCTT GATTCTCCAT ATGTTGTTTC TTCAAAATGT 
ACTGGAATAT ATAAAGTATA TGAAACATTT GATCCTATAA AATATCTTAC GGATATTCAT 
AAGGAAGCTT TCGAGGCAGT GATGCTAATG TGGGGAAAGA AACCTTTAAA AAATTATGGA 
GTTCATATGA CAGAACTTAT TTTATCTATC TTAAGACATA TTTTACGAAG TGAGCAGATC 
ATAAAGGAAC ATGCTGAAAA AGAAGAAAAT AATGAAGGAG GTATTAGTGG TAGTACTAGT 
GGAACTAGTG GAATCGGAAG AGGTGGAACA ATATCCGATC GCAGAGAAGA ACCAGAAGCA 
GATGTAAATC CAGAACATCT AAGACAGTTG ATGGATATGG GTTTCAGTAG AGCTCATTGC 
ATAGAAGCTT TGCTTCATAC ATTGACAGTT GAACAAGCTA CTGATTATTT GTTAACTAAT 
CCTGCTACTC TACGTCGATC AGATGTGCCA GCAGGTGATG TAAGCGGACA AGATTTAATA 
ATGGATTTAG AATTGGTAGA TGATGATCAA ATAATGCATG CCATAGCGAT GTCGCTTGGA 
GAAACGGCGG AAACGCGAAA TGTTTTCAGC ACAGAAGAAT CCAGTTCACG TGAGGCTACT 
ACCATAACTG AAAGCATAGA TAAATTTACA CAAAATGCTT TAAGTCAATG TTTAAATTTA 
TTGGATCTCA TGCCTGATAC TGTATACAGA ATTTGCGATT TATTAGTTGC CATCGCTAAA 
AGAAACGGAG ATGAATGGCG TGATAATATG TTGCGACAAC TTATGAAAGA AATTGGAGGT 
TTGGTAGATT ATTTAATCGA AGTTGCTGAA AGTCAAGACC CGAATGCTGG AGTAAAATTA 
GTAGAATGTA AAGAAGCTAA TAAAGTTACT GGACGTATTT ATCTGTATAC AATGTTCTTT 
GAAGGAACAT TCCAAGAAAT GCGAGTGCCC TGTGCTCAAA TTGTAGAAGA ATATGCTATT 
TTAAATAAAC TTGTACGTTT ATTAGTCGTA AGTGAGGGTC CGTTAACTGC AAGTAAAAGT 
AAAATTGTTA AGGAAGGTAA TAAGGATACA TCATCTACGA AAACTAGAAA ATGGCTTGCA 
CCTTTGTTAT TGCTGATAGA ACGTTTAGAA AAAGTAGCGG TATTGACAAA GAGAAAAGAA 
TTAATGTATA AAGTAACAAC CAGGATGTGG AAATGGTTCG ATCTCAGCAC AGGAAAATGG 
AATTTATATT CTCCAGTAAA TAATCGAATA ATTAATGATG CGTATTGGGC TGGCGAGTCT 
AGTACGAGAG TAACATGTAA CAGGCGACGA TATGTAATTA ATTTTTCATG CATGACTCAA 
GTTAATGAAG AAACTGGTAA TAGAAGACCT ATTACGATGG GCTTTAAATT GCATACCGGA 
AATGAAGATG GTGGATCAGG TTCTGATACA AATATGGACA CTGACGAAAA TCGAATAGAA 
GATAAACGGA ATACAGTTTT GCAAGGGTTA GATCCTAGCA TGGCTCCAAG TATCATACGC 
GCTTGTGTAA GATTGATGGC TATTCCAGTA GATCGAGATG CTTTACATGC AGCTATGAAA 
GTTTGTTTGA GACTTACAAG AGATTATGAA AATGCCGAAA TATTTGTTCG TGAGGGTGGT 
GTGAAACTTC TTCTAGAAAT GTCTCAAGCA AGTAGTTTCA TTGGATGCAT AACTTTAAGT 
ACTCTTTTAA TAAGGCATGC TTTGGAAGAA CCATGTACTC TTCGATTAGC CATGGAAAAA 
GTAATTCGTA GTCGTACTCA AGGCAATATT CCTCCAGCTT GCAAAGAAAT CTTATTTATG 
ACACGTCAGA TTAATAGCGC TGTATGTCGC AATCCGGAAG TTTATAGAGA AGTTTGTGAA 
AACATTTTAA GAGTAGATAT TCGTCTATTA GGAAAAGAGG ATGATGATAA TAGATTAATT 
GTCAAGGCAC TTCCACCAAG TCACTCATCA GTAATGCCTA TGGTAGAGAA AGCTTCAATA 
TCTGTTGTAA AAGATCTTCT GAATGCTCTT ATAAAGCCTA TGCCAGTTGT CTCTGATGAA 
AAGTCTTCTA CACCAACAAG GAGTCCAGAA AAGAAGACAT CTTATTCTAA TGTTGGAAGT 
TCATCTTCTC GACGAGATGT TCTTAGAAAT AATGCAACAA CATCAAATGA TACTCTTGAT 
GATGAGGATC AAGTTTCTCA AATAATACCA ATGAAAATTT ATCCAGATAA TAGCAATAAC 
AGTAAAGTGG AACCAGAAGA AGCAGAAAAA CCTTTATTAA CAAAATCTGC TATTTTGAAG 
ATACTTGCAG AAGCCGTTCG CTCATATAGT GCAGTCGCAA GTTTAATTAC AGAGCATGCA 
TATCGAGCCG GACAAAGTGA AATGGTTCCT GAATACACAA CAGCTCTTGC TTTTCTTTTA 
GATAAGCTTT TACCAATGTC ACCAGAAAAT TCAAGTGACA AACAATGCAG CAGTATGGCG 
AGAATGTTAA TAGCTGCTAT TGCTTCCTGT AATCATTCTC CGGATGTTCA AACAACTTTG 
GTGACTGAAG TAAAAGCAGC TTTAACAAGA ACTTTAGCTC TTCCTGAAAG TTCTGAGAAA 
CATGCACAAA TTCAATTATT AGTTGGATTG ATCTCTACTA TGATTGATAG TTGTCCACCA 
ACTTCTTACA ATCAATTGAG GACTTCCTTG AAGATGCATC AATACAATAA TGTTAACTAT 
ATTGTAAGAA TCATGTTGAG AAAAGGAATA ATTACAGATT TAGCAAAAAT TCCACATAGT 
CTGGATTTAT CAAGTCCAAA TATGGCTGCT ACCATAAATG CTGCTTTAAA ACCATTGGAA 
ACGCTTTCGC GAATTATAAA TCAACCAATG CCAGGTGCTA TTAATAACAA ATTTACAAAG 
CCAAAAAGTA GAACAGTTCA AGAGGAACCT ATTGGAGAAC AAACAGGAAC TACAACATCA 
GAAGCGACAC ATGCTGTTGG CGAAGAAACG AACGAAGACG CAGAGAATAC AGAACACGAT 
ATATCTGTTA CTGCGGAAAG TCTTGAACCT ACTTCAGAAA GTCAAGTACA TGAAGATGAA 
GCTGCTTTAG AAGACATTAT GGATCAACTG CTTGAAAGTG TACTATTTTC CAGGGACGGG 
CCGGGCGTGA CGGAAGATCA ATCTTCTCGA CCACATAGAC CAATGGACAT TGATGAAGAA 
AGCCTTGCAA TAAGAGATGG AGAAGCTGAT TTTGATCCAA CACGAGATGC TACGGAGGAT 
TTAATGAGCT CTGATTCGGA TTCTGATAGC AATCCATCTG ATGATAATGA AGATGAAGTG 
CAAGATGATG AAGATGAAGA AGAAGAAGAA GAGGATGATG GAGAAGAAAA TGAAGAAGAA 
GAGGAAGAAG AAGAAGAAGG ATCCTCAAAT TACGAAGAAG ACGGAATTGA ATTCTATGAA 
GATGTAGGAG AAAGTGGTGT ATTCAGAATG ATTCCATCAA CAGATCGTGA TGGTAGTAGT 
GTCGTAATGA TTCGACATAA TATAGATAAT CAAGACAATG TGAATTCTTC AACTACAGTT 
ACACCACGAT CTAGTCTTCC ATGGAGTACA ACATTTCCTC TTCCTTTGGG ACTATTTGAA 
AATCCTCCAT CAATTTCTGA AAGTAGTGGT ATTAATTCCC ATTCGTTGCT AATACCACAG 
GGAGGTTTAG ATGCTACTAA TACAAGAGGT CAGAGAGCTG TACGACCGCG AAGATATCAA 
TATCTTCAAT TACCAAATCC ACGAAATCCA AATCCACCTG TAATATTGCA AAGACTTTTA 
GGTCCAGCTG CACAAGCAAT TCCATTATCT GCCAGTCAGG TTTTGGCTTC CAGTTTTCGA 
GATACACGCG TTGTAGTAAT GGATAATGGT GTTGGAATAT TAACAAATCA AGAAGAAGAA 
CAAATAGATT TTGTGGATCA ATCTGGCTAT CTTTTCGGAT CTAGTCTTGC AACCACAGTA 
AATAATACTC CTACTGCTTT ACATTGGTGG ATTGAAGAAA GCAAACTATT AGATGGAGAT 
TCCCAAACGG ATTGCTGTGT TGGTGTTGTA CATAAACTAA TTCCTGGATT GGAAAAACGT 
AGAAATGCAG AATTGGGAGA ACGACGTGAA AGACGTAAAA TGCAATACGC TTCTGAGAAA 
AAAAATGAAA AAGATAACAA AGATGGAAAA GATCATTTAA ATAATACAGA ATCTGGTTCT 
TCTACTATAA TATCTATGGA TGAACAAATA TCAACTGCAT CAACTTCACA AAGAGAAGAA 
ACGAATAATT TTGTTCCTAG ATTTGAAACT TGCGTGGAAA CAACTCGATT AGAACGCACA 
CTTGACGAAG ATATAATTGA TGTAACTGGT GAGATGGATG CAACCATTCA AGAAGATGAA 
GAACGACCAC CACCTCCCCC ACCAGAAGAA CAACCAACAG AACCTAGAGA TCCTAGAATA 
AGCAATCGAA ATCCAACTTT GGAACTTGCT GAAAGTATAG TGGATTCTGT ACTTGGACCT 
TGCGCGTCTG AAGCAAGCAG ATTAAGACAA TCTGAACGTA CCGTTGAAGG ATCTAATACT 
AATGAAACAT CTAGTCGACC CAATACGTCC ATAGTTGTTG ATTTCAGTCA AGAACCTAAT 
GAAGATCTAT TAAGAGAAAG TGATTCATCT GAATGGATAA GAATTCTTAC GGATGACAGT 
CAATCAAATG TTGAACGAAA TGCGAATATT CTCAATCAAG TACCTACCAC TTCCGCATCA 
GAAAATATTA ATCCAGAAAT ATCAGAACAA CAACAGCAAC AGCAGCAGCA ACAACAACCA 
TCTATACAAC AGCAGCAACA ACAAGAGCCA CAACAATCAC AAGAACAGTC GCAACGACAA 
CAACAAAACC AACAATCTCA AGAACAACAA TTACCGGATG GTGTAGATCC TTCTTTCTTA 
GCTGCATTAC CCGAAGATAT GCGTGACGAA GTTATTGCTG AGCAACTAAG ACTTCAACGA 
ATTCGGCAAA GAGCACAGGC AACTGTAGTC GAAGAATTAA CAGGACCGGT TGAAGTTAAT 
CCTGAATTTT TAGCTGCTTT ACCTCCAGCA ATACAAGAAG AAGTTCTTGC GCAACAACGC 
TTGGAACAAC AACGTCATGC AGCCGCAAGT GCCAATCCAG AAGACCCAGT TGACGCAGCT 
GCATTCTTCC AAAATTTACA ACCTTCTCTT CGACAAGCGA TTTTAACTGA TATGGAAGAA 
TCGCAAATAT CCGTTCTTCC ACCAGACTTA GCTCAAGAAG CTCAAAATCT TCGAAGAGAA 
TGGGAAGCTC GAAATAGACA TATAATGCAA GATCGATTTC TTAATCATGT TAGTCAAGGA 
AATGCCGCCT TATCTAGTAT ATTGCGAAAT TCTGGAAGAG GTAGAGGTGG TGGAACGCGT 
TATGCAATTC ATACAGTAGC ACAAAGAGGA TGGCAACCAT GGTCAAATGG TGATCCTAGT 
ATAACGCATC ATGGAGCGGG TCTACGACTC AGAGGCAGAC AATTATTGGA TCATGAGTCG 
ATGGCTTGCG TATTGGTATT ACTGTTTGTT GATGAACCAA AAATTAATAC ATTAAGGCTT 
CATAGAGTTA TCAGGAATCT TTGCTATCAC GGTAGCACAA GGGAATGGGT AGTTAGAGCT 
TTACTAAGTA TCATGGAAAG AAGTAATGAT GAAAATAAAG ATATAGCCAT GGACTTTGGT 
GGAAAAATCA GAAGAAAAAG CTTAGTGCCC ATGATGCATC ACGATTATTG TGCTCCTAAA 
ATTTTTGATC AAAGAAGCAA TGCACAGTCA TGGTTAAACA TCAGTATTGA TGCTGCGCTT 
GGATGTAGAG CAAATGTTTT CCATATTATT AGACATGGAG GCAAAAAATC AGAAAGACAA 
GGCAGTAGTA TTGCCATCCA TCCTCAAGCA GCTCCTACAG TATGCAGGCA TACATTAGAA 
TTATTAATAT CATTGGCTAA AGGCTTTCCT GGTTACTTTG TACCAATCAA GTCTAAAGAA 
TCAGATGAAA AAGAAAGTAA CAAAGAAAAA GATTCAAATA ACAAAGAAGA AGTCGGTTCG 
AAAGGTAAAT CGACATCGAA ACCAGGAAAA AGTGATCAAC CTGATTTTTG GGATATGTTG 
TTGAAACTTG ATTCATGTGC TTCGAAAAAA GGAAAATCAG TTGCTAGATC GCACTCAAAT 
TCAAATTTAG GAAATGATAC GGAACATAAT ATCACAACTT TTGAGTCATC CGCTTTCGGT 
CAATTAATTT CAATGCTAAA CTGGTCGGTT ATTAAACGTA GCAGTCAATT AACGGACAAA 
GTGTTGAAAT TATTGTCTTT AATTTCAATC GGATTAACGG AAGTGAATCC GTATCGTAGA 
CAAGAAGGAA ATAAGAATAA AAGAACAGAT GTGAATAAAG AACAAAGTAT TGCTGCACCA 
GAAGAACATC TTAAATTGGC TGTGCAAGTA TTGACAAGTA AGAGTTGCTC TGAAGAAGGA 
CTTGAAGATG CAACAGCTTT GTTGCTTAAT TTATCTCATT GTCCTGATCC TACTAGACAA 
TTGATATTAA AATTACTCTT AGAAGGTGCC ATGGAATTGG CTAATGTTGT TTGTGAACAC 
ATTAAAGATT TATTAATGGA GTTAAAAGTT TTGAATCGTG AACTTAGACG AAATTCTATC 
GAAGAGCAAC CGTCAACTAG TACTGGAGGT TCTCGTTGGG TTCTTTTAGA TAGGTTTACC 
AACGAGAGTG TAGTTATTAC TGCGCCGAGT AAAGTTAAAG CTGGCAGCGA TCTTCAATTA 
CCATCGATGG CTGCTCTTGT TAGCAAAACT TCTAGTCAGG CATTTTTCTT AAGAATACTT 
AAAGTGATTG TACAAATAAG GGAAGCTGTA CGTTTAGCAA ATAATAAAAA AACATCCACT 
CAAAGTTCCG AAAGTTCTGT AGATACTGAA AATACTAATC AAGCTTCAGA ATCAGGTGAA 
GATAAGCTTC CATTATTAAG TGAAAGTTTA GTATTAGATC ATCTATGGGA AACACTTAGT 
GCTTGTTTAT TGGAATTGGA ATATACTCCA GATCAACATG CAGTTTTAGT ATTGCAACCT 
GCAGTAGAAG CATTTTTCCT AGTACATTCA TCATCGAGTA CTTCTCGTGA TCGTCATCTA 
AGTACAAGTA CGGAAAATCG CGAAGTAGTT CCTGATCTAA CACCTGTTTC ACCAATTTAT 
TCAGATAATG AAGGAACGTC CCAATCAGAA GCTACTATTA ATACTTCTTG GGATATACAA 
CCTACGCCAC AGAAGACATT ACCACCGGAC CAACTCAAGT TCCTTAAATT TGCAGAAACG 
CACAGAACCG TTTTAAATCA AATTCTTCGT CAAACAACTA CGCATTTAGC AGATGGACCA 
TTCTCTGTGT TAGTGGATCA CACAAGAGTT CTTGATTTTG ACGTAAAAAG ACGATATTTC 
CGAACAGAAT TAGAACGAAT GGATGAAGGT ATTCGAAGGG AAGAATTGGC AGTACATGTT 
CGTAGGAATA ATGTATTTGA AGATTCATTT CGAGAACTTC ATAGGCGTAA TGCTGATGAA 
TGGAAAAATC GTTTTTATAT TGTTTTTGAA GGTGAAGAAG GGCAAGATGC TGGAGGATTA 
CTTAGAGAGT GGTACGTTAT TATTTCCAGA GAAATTTTTA ATCCTATGTA TGCATTGTTT 
ACTGTAAGTC CTGGCGATCG AGTTACATAC ATGATAAACT CCTCTTCACA TTGTAATCCT 
AATCACTTAT GTTATTATAA ATTTGTCGGT CGAGTTATAG CTAAAGCAAT TTATGACAAC 
AAATTATTAG AATGTTACTT CACGCGTAGT TTTTATAAAC ACATTTTGGG AATACTTGTG 
AAACATACAG ATATGGAAAG TGAGGATTAT AGTTTTTACA AAGGACTTGT ATATCTCACA 
GAGCATAATA TTGCTGATCT TGGATACGAA TTAACTTTCT CTACAGAGGT AAACGAATTT 
GGTGTAAATG ATGTACGTGA CTTAATACCA AATGGACGTA ATATCATTGT TACTGAGGAA 
ACAAAATTAG AATATATTCG GCTCGTATGT CAAATGAAAA TGACTGGAGC AATTCGTAAA 
CAATTAAATG CGTTCCTAGA AGGTTTTTAT GATATTATTC CCAAACGACT GATCTCTATA 
TTCAATGAAC AAGAACTTGA ATTGTTAATT AGTGGATTAC CCAATGTAGA TATTGAGGAT 
CTTAAAGCTA ATACTGAATA TCATAAATAT AATGCAAATT CTTTACAAAT TCAATGGTTC 
TGGAGAGCAT TACGAGGTTT CGATCAAGCA GATAGGGCTA AATTTTTGCA ATTTGTTACT 
GGAACTTCAA AAGTGCCACT TCAAGGTTTC GCTGCTTTAG AAGGAATGAA TGGTATACAA 
AAGTTCCAAA TTCATCGTGA AGATAGATCG ACAGATAGAC TTCCGTCAGC ACATACATGT 
TTCAATCAGC TAGATCTACC AGTTTATGAA ACATACGATA AGCTTCGCAC GAATTTATTA 
AAAGCGATCC ACGAATGCAG TGAAGGATTC GGTTTCGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC411961 ( XM_026442187.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_026297972.1 Apis mellifera E3 ubiquitin-protein ligase HUWE1 (LOC411961), transcript variant X2, mRNA.

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CloneID	OHd32886
Clone ID Related Accession (Same CDS sequence)	XM_026442187.1
Accession Version	XM_026442187.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 12447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1537200000000
Organism	Apis mellifera(honey bee)
Product	E3 ubiquitin-protein ligase HUWE1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037645.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATTG ATCGCAATAG ATTGAGAAAA TATACATCAG AAGCGCCTGC AAAATGTCAG 
GCGCTTATAA ACAAGTTGCG CTCATGCTCC CATGCAGAAT TGCTAGAGGA GCTAAAGCAA 
ATAGATGCAT GGACTTTTGG TAAATGTGAA TTATTTCATT GGGTCGATGT ATTGGACCTT 
AGTGACAGCA TTTTGGAAGA AGCTGCTGCT AAAAGCCCAG ATAATCCATG GGAATTGGCT 
TGTGATCTCC CTCAAAATAG AGAGGCAAAA GAACTTCTCC TTTGGGTTCT ACATCTTACA 
ACATTATTGA TTGAACATTC ATATTCGCGA CATTTATACA GCAGTATGGA ACATCTAGTG 
ACTTTATTAT CAAGCTGTGA CATGCATGTT GTCCTTGGTG TATTAAATAT TCTCTACATG 
TTCAGTAAAC GTAGCAATTT CATTGCACGT TTAAATAGTG ACAAGAGACA AGCTTTATTA 
AGTAGACTTA CTCTTTTAGC AGAGAGTTGG GGAGGTAAAG AAAATGGATT TGGTTTAGCA 
GAATGTTGTA AAGAATATCC AGATAAGCCA CTTCCAGCAA GTGCCACAAC ATTGCATTTC 
GAATTTTATG CAGAAAATTC AACGACAGAT TCAAATGCCA CATCAAACAT TGGCACAAAA 
AAATCTGGAC AGACGAATTT TGTAACATGT ATACATATAG AAAATGTAGA TAAACTGGGT 
AAAACACCAG CACAAATTAT GAATGATTTG TTAAAAGTCA ATAACGTACC CCAAGATCGA 
CAGATTGCGT TATTTACACA TATAAGATTA GCACACAGTT TCTCTGACTA TAGAAGACGA 
TTACAATGTG TGCACGCTCG GCTACAGGCA TTATCAATAC TTGTATATAA TAGTGAACTA 
GAAGAAAATG CACATAGTTT GTTGCATCCT GGACTTTTAG AAGAATTAGT TGAATTACTG 
GAACTTCCAC ATGCACATCT TGTTGAATTA CGTGCTGCTG CTTTAAGAAC TCTTACAAGT 
ATCATTCATT TAGATCGCAA TCCACATTTT CCAAAAAAAC CTAGTTCAAG ATTAAATATG 
ATAATCGATG TCACTGGAGC CAATTCATAT CATGGATTTT TTCCTGTATT AATTCGAACT 
TGCATTTCAA CATTAATTTC TCCACAACCT GAAGGACAAC CATTTCCTTT GTCACTTGCA 
ACAGCTTTAT TTAGCTTTCT TTACCATCTT TCAAGTTATG AAGCAGGATG TGAAGCACTT 
GTTAGTTGTG GGATGATGGA AAGTTTGTTA AAAATCATGA ATTGGCCTGG TAGCGAGTTT 
GAACATTTGA CGTTTGTAAC AAGGGCAGTT CGTGTTATAG ACTTAATAAC AAATATAGAT 
ATGCAAGGAT TTCAAGCTCA TGGTGGTTTA GCCAGTTTTA TTAATCGTCT TGATATGGAA 
GTAAATGTTT GTAGAAAAGA ACAACCATTT GAAATAGAAC CAATACATTA TCAAGATAAT 
TCTCAGGCCA CTCGTGAAAG ATCAATGGAT CGCGATGAAA GTTCTACGTC TTCACGTGCT 
TTTGATGAGC GTGAAGAATC ATCTAATCCT ATGGAATTTT CGGAAGATGA GAACATGAGT 
TCCAAAACCG ATGATAAAAA TGAACAAATA CCAGATTATT CAGCTGGAAA AACTGGAAAA 
ACTTGTCTAC CACAGCGAGT TACACTTTTA AAAGCGTTAT TAAATTTTAT TAAAAAAATT 
ATTCAAGATC CTACATTTTC TGATAGTATT AGACATGTTA TGGAGGGTAC TTTACCTTCT 
TCCTTGAGAC ATATTATCAG TAATTCAGAA TATTATGGTC CTTCTTTGTT CCTTATTGCA 
ACAGATGCTG TAACAGTATA TGTTTTCCAA GAGCCATCTT TATTATCTTC TTTACAGGAT 
AATGGATTAA CTGATCTTGT ATTGCATGCT TTACTAATTA AAGAAATTCC TGCTAGTAGA 
GAAGTTCTAG GATCATTGCC TAATGTATTT AGTGCTTTAT GTTTAAATCA GCGTGGACTA 
GAATCATTTG TAAAACGACG TCCTTTCGAA CGCTTATTTA AAGTATTATT ATCGCCACTT 
TATCTGCCTG CAATGAATCG ACGTCGTAGC ACAGATCCTT TAGGTGATAC TGCTTCGAAT 
TTGGGTAATG CAATGGATGA ATTAATGCGA CATCAGCCAA GTTTGAAGGT TGATGCAATC 
GCTGCTGTTA TAAAGTTATT AGAAGAACTT TGTGTACTGG GTTGTGATCC TCGTTATATA 
TGCTTGCAAG CTGAAGATAA ATTCGATAAT TCTCAATCAA ATACTACTTC TGGAAATCGT 
CAATCTTCTG GACAATCTGT TGGAGTTGGA GTTGGAGATT CTGGTGTAGG AGGAGGAGGT 
GGTAGTGGAA GTAGTAGCGA CGAAGAAGAA GAAGATGAAG AAGAAGCTAG TACAAGTTCT 
CATCCACAAC GTGAAGAACA ATGTTCATCA TCCACTGCTC AACCAGTTCC ACCACCTCAA 
CCTAGAGAAA AAACTCCAAT AGCTTTAATG GAATATATAC AAAATGTTAC AAAATTCGTT 
GATGCTATTC TCAGTAATAA TTCCACGGAT GATCATTGTA GAGAATTTGT CGCCCAAAAA 
GGTCTCGTAC CATTATTGTC AATTTTAGGA TTACCTAATC TTCCAGTGGA TCATTCAGTT 
GCCCAAACGG CACAAGCTGT AGCTACAGTT TGTAAAAGTA TTTTAAATCT CGCACATGAA 
CCATTAGTTC TTAAAACTGG TCTATCTCAG TTGAATGAAG TTTTGAATTT GCTGAAACCA 
TTATACAATT ATATGGAAGC TCCAGGAGGA TCTGTTTTAT TACATGAACT TGTCAATGCT 
CCAAATCTGG AAACAGCTTT TACCAGTGAT ACGGCAACAC CTTTACTTCA TGCTATGAAT 
GCTGCTTATG GATATATAAT AATGTTTGCA CAAGTGTGCC GTACCAGTCA AAGTGAAATC 
AGAAACTTGG CTATGAATCA TTGGGGTTCT GAACTTGGTT TGACTGTTCT GAAAGGTTTA 
TCAGAATTAT ATACAGCTCT AATCTGGGAA AGTACAGCCT TATTAGCACT TACCACGGAA 
GATTTTATTC CGGCTGGTTG TGATTTTGGA AAAGAAGATA TGGATAAACT TATACGTCCT 
GAATCAACAA ATGAAGGTGA AAGTACTAGT TGGTCTGGTG CTACATCTAC AGATATGGGA 
AGTAATGGGA TGACTACAGC TATGGAAGCT TTAAGCACTG ATCCGCCTCC TATACCTATG 
GAAGTAGACG AAAAACCAAG TGTTAGTACA GGAAGAATAT CTCCAAATTC TCATCGACTT 
ACATATCCTA AACATTTGCT GGGACTTACA TCTAAAATGG GCAGAGCTTT AGCAGAACTC 
TTTGCTATAC TTGTAAAGCT TTGCATGGGC TCTCCAATTA GACAACGCAG AGGACAACAG 
ATGCCTCCAA TACCAGCCTT ACCTTCACAA GCATCTAGAA GTGTAGCAAC TGCTTTAAAT 
TGTGTATTAA TTCGCGGATT ATCATGGGAT AAATTACCAC CATCGACAGT GCCAAAACTT 
CAAGTAACCT TTTTGATGTG CACTTTAAGT TTTGTATCTC CTGCATTATT TGATGAAAAG 
GGGTATCCAT TTCATTTGAT GCTTCAAAAG TTCGTTAAAC TCGGCGGACA AAATGCATTT 
TTCGCTGCTT TTCATTGGGT ATTATCTGGA GGAGGACAGT TTCCGTTATC TGAAGGATTA 
GAGCATCCAA ATTTACCTGA TTGTACTGGA GAATTCTTAG ATGGTTGGTT GATGATTTTA 
GAAAAAATGG TGAATCCTAA AACTCTTCTT GATTCTCCAT ATGTTGTTTC TTCAAAATGT 
ACTGGAATAT ATAAAGTATA TGAAACATTT GATCCTATAA AATATCTTAC GGATATTCAT 
AAGGAAGCTT TCGAGGCAGT GATGCTAATG TGGGGAAAGA AACCTTTAAA AAATTATGGA 
GTTCATATGA CAGAACTTAT TTTATCTATC TTAAGACATA TTTTACGAAG TGAGCAGATC 
ATAAAGGAAC ATGCTGAAAA AGAAGAAAAT AATGAAGGAG GTATTAGTGG TAGTACTAGT 
GGAACTAGTG GAATCGGAAG AGGTGGAACA ATATCCGATC GCAGAGAAGA ACCAGAAGCA 
GATGTAAATC CAGAACATCT AAGACAGTTG ATGGATATGG GTTTCAGTAG AGCTCATTGC 
ATAGAAGCTT TGCTTCATAC ATTGACAGTT GAACAAGCTA CTGATTATTT GTTAACTAAT 
CCTGCTACTC TACGTCGATC AGATGTGCCA GCAGGTGATG TAAGCGGACA AGATTTAATA 
ATGGATTTAG AATTGGTAGA TGATGATCAA ATAATGCATG CCATAGCGAT GTCGCTTGGA 
GAAACGGCGG AAACGCGAAA TGTTTTCAGC ACAGAAGAAT CCAGTTCACG TGAGGCTACT 
ACCATAACTG AAAGCATAGA TAAATTTACA CAAAATGCTT TAAGTCAATG TTTAAATTTA 
TTGGATCTCA TGCCTGATAC TGTATACAGA ATTTGCGATT TATTAGTTGC CATCGCTAAA 
AGAAACGGAG ATGAATGGCG TGATAATATG TTGCGACAAC TTATGAAAGA AATTGGAGGT 
TTGGTAGATT ATTTAATCGA AGTTGCTGAA AGTCAAGACC CGAATGCTGG AGTAAAATTA 
GTAGAATGTA AAGAAGCTAA TAAAGTTACT GGACGTATTT ATCTGTATAC AATGTTCTTT 
GAAGGAACAT TCCAAGAAAT GCGAGTGCCC TGTGCTCAAA TTGTAGAAGA ATATGCTATT 
TTAAATAAAC TTGTACGTTT ATTAGTCGTA AGTGAGGGTC CGTTAACTGC AAGTAAAAGT 
AAAATTGTTA AGGAAGGTAA TAAGGATACA TCATCTACGA AAACTAGAAA ATGGCTTGCA 
CCTTTGTTAT TGCTGATAGA ACGTTTAGAA AAAGTAGCGG TATTGACAAA GAGAAAAGAA 
TTAATGTATA AAGTAACAAC CAGGATGTGG AAATGGTTCG ATCTCAGCAC AGGAAAATGG 
AATTTATATT CTCCAGTAAA TAATCGAATA ATTAATGATG CGTATTGGGC TGGCGAGTCT 
AGTACGAGAG TAACATGTAA CAGGCGACGA TATGTAATTA ATTTTTCATG CATGACTCAA 
GTTAATGAAG AAACTGGTAA TAGAAGACCT ATTACGATGG GCTTTAAATT GCATACCGGA 
AATGAAGATG GTGGATCAGG TTCTGATACA AATATGGACA CTGACGAAAA TCGAATAGAA 
GATAAACGGA ATACAGTTTT GCAAGGGTTA GATCCTAGCA TGGCTCCAAG TATCATACGC 
GCTTGTGTAA GATTGATGGC TATTCCAGTA GATCGAGATG CTTTACATGC AGCTATGAAA 
GTTTGTTTGA GACTTACAAG AGATTATGAA AATGCCGAAA TATTTGTTCG TGAGGGTGGT 
GTGAAACTTC TTCTAGAAAT GTCTCAAGCA AGTAGTTTCA TTGGATGCAT AACTTTAAGT 
ACTCTTTTAA TAAGGCATGC TTTGGAAGAA CCATGTACTC TTCGATTAGC CATGGAAAAA 
GTAATTCGTA GTCGTACTCA AGGCAATATT CCTCCAGCTT GCAAAGAAAT CTTATTTATG 
ACACGTCAGA TTAATAGCGC TGTATGTCGC AATCCGGAAG TTTATAGAGA AGTTTGTGAA 
AACATTTTAA GAGTAGATAT TCGTCTATTA GGAAAAGAGG ATGATGATAA TAGATTAATT 
GTCAAGGCAC TTCCACCAAG TCACTCATCA GTAATGCCTA TGGTAGAGAA AGCTTCAATA 
TCTGTTGTAA AAGATCTTCT GAATGCTCTT ATAAAGCCTA TGCCAGTTGT CTCTGATGAA 
AAGTCTTCTA CACCAACAAG GAGTCCAGAA AAGAAGACAT CTTATTCTAA TGTTGGAAGT 
TCATCTTCTC GACGAGATGT TCTTAGAAAT AATGCAACAA CATCAAATGA TACTCTTGAT 
GATGAGGATC AAGTTTCTCA AATAATACCA ATGAAAATTT ATCCAGATAA TAGCAATAAC 
AGTAAAGTGG AACCAGAAGA AGCAGAAAAA CCTTTATTAA CAAAATCTGC TATTTTGAAG 
ATACTTGCAG AAGCCGTTCG CTCATATAGT GCAGTCGCAA GTTTAATTAC AGAGCATGCA 
TATCGAGCCG GACAAAGTGA AATGGTTCCT GAATACACAA CAGCTCTTGC TTTTCTTTTA 
GATAAGCTTT TACCAATGTC ACCAGAAAAT TCAAGTGACA AACAATGCAG CAGTATGGCG 
AGAATGTTAA TAGCTGCTAT TGCTTCCTGT AATCATTCTC CGGATGTTCA AACAACTTTG 
GTGACTGAAG TAAAAGCAGC TTTAACAAGA ACTTTAGCTC TTCCTGAAAG TTCTGAGAAA 
CATGCACAAA TTCAATTATT AGTTGGATTG ATCTCTACTA TGATTGATAG TTGTCCACCA 
ACTTCTTACA ATCAATTGAG GACTTCCTTG AAGATGCATC AATACAATAA TGTTAACTAT 
ATTGTAAGAA TCATGTTGAG AAAAGGAATA ATTACAGATT TAGCAAAAAT TCCACATAGT 
CTGGATTTAT CAAGTCCAAA TATGGCTGCT ACCATAAATG CTGCTTTAAA ACCATTGGAA 
ACGCTTTCGC GAATTATAAA TCAACCAATG CCAGGTGCTA TTAATAACAA ATTTACAAAG 
CCAAAAAGTA GAACAGTTCA AGAGGAACCT ATTGGAGAAC AAACAGGAAC TACAACATCA 
GAAGCGACAC ATGCTGTTGG CGAAGAAACG AACGAAGACG CAGAGAATAC AGAACACGAT 
ATATCTGTTA CTGCGGAAAG TCTTGAACCT ACTTCAGAAA GTCAAGTACA TGAAGATGAA 
GCTGCTTTAG AAGACATTAT GGATCAACTG CTTGAAAGGG ACGGGCCGGG CGTGACGGAA 
GATCAATCTT CTCGACCACA TAGACCAATG GACATTGATG AAGAAAGCCT TGCAATAAGA 
GATGGAGAAG CTGATTTTGA TCCAACACGA GATGCTACGG AGGATTTAAT GAGCTCTGAT 
TCGGATTCTG ATAGCAATCC ATCTGATGAT AATGAAGATG AAGTGCAAGA TGATGAAGAT 
GAAGAAGAAG AAGAAGAGGA TGATGGAGAA GAAAATGAAG AAGAAGAGGA AGAAGAAGAA 
GAAGGATCCT CAAATTACGA AGAAGACGGA ATTGAATTCT ATGAAGATGT AGGAGAAAGT 
GGTGTATTCA GAATGATTCC ATCAACAGAT CGTGATGGTA GTAGTGTCGT AATGATTCGA 
CATAATATAG ATAATCAAGA CAATGTGAAT TCTTCAACTA CAGTTACACC ACGATCTAGT 
CTTCCATGGA GTACAACATT TCCTCTTCCT TTGGGACTAT TTGAAAATCC TCCATCAATT 
TCTGAAAGTA GTGGTATTAA TTCCCATTCG TTGCTAATAC CACAGGGAGG TTTAGATGCT 
ACTAATACAA GAGGTCAGAG AGCTGTACGA CCGCGAAGAT ATCAATATCT TCAATTACCA 
AATCCACGAA ATCCAAATCC ACCTGTAATA TTGCAAAGAC TTTTAGGTCC AGCTGCACAA 
GCAATTCCAT TATCTGCCAG TCAGGTTTTG GCTTCCAGTT TTCGAGATAC ACGCGTTGTA 
GTAATGGATA ATGGTGTTGG AATATTAACA AATCAAGAAG AAGAACAAAT AGATTTTGTG 
GATCAATCTG GCTATCTTTT CGGATCTAGT CTTGCAACCA CAGTAAATAA TACTCCTACT 
GCTTTACATT GGTGGATTGA AGAAAGCAAA CTATTAGATG GAGATTCCCA AACGGATTGC 
TGTGTTGGTG TTGTACATAA ACTAATTCCT GGATTGGAAA AACGTAGAAA TGCAGAATTG 
GGAGAACGAC GTGAAAGACG TAAAATGCAA TACGCTTCTG AGAAAAAAAA TGAAAAAGAT 
AACAAAGATG GAAAAGATCA TTTAAATAAT ACAGAATCTG GTTCTTCTAC TATAATATCT 
ATGGATGAAC AAATATCAAC TGCATCAACT TCACAAAGAG AAGAAACGAA TAATTTTGTT 
CCTAGATTTG AAACTTGCGT GGAAACAACT CGATTAGAAC GCACACTTGA CGAAGATATA 
ATTGATGTAA CTGGTGAGAT GGATGCAACC ATTCAAGAAG ATGAAGAACG ACCACCACCT 
CCCCCACCAG AAGAACAACC AACAGAACCT AGAGATCCTA GAATAAGCAA TCGAAATCCA 
ACTTTGGAAC TTGCTGAAAG TATAGTGGAT TCTGTACTTG GACCTTGCGC GTCTGAAGCA 
AGCAGATTAA GACAATCTGA ACGTACCGTT GAAGGATCTA ATACTAATGA AACATCTAGT 
CGACCCAATA CGTCCATAGT TGTTGATTTC AGTCAAGAAC CTAATGAAGA TCTATTAAGA 
GAAAGTGATT CATCTGAATG GATAAGAATT CTTACGGATG ACAGTCAATC AAATGTTGAA 
CGAAATGCGA ATATTCTCAA TCAAGTACCT ACCACTTCCG CATCAGAAAA TATTAATCCA 
GAAATATCAG AACAACAACA GCAACAGCAG CAGCAACAAC AACCATCTAT ACAACAGCAG 
CAACAACAAG AGCCACAACA ATCACAAGAA CAGTCGCAAC GACAACAACA AAACCAACAA 
TCTCAAGAAC AACAATTACC GGATGGTGTA GATCCTTCTT TCTTAGCTGC ATTACCCGAA 
GATATGCGTG ACGAAGTTAT TGCTGAGCAA CTAAGACTTC AACGAATTCG GCAAAGAGCA 
CAGGCAACTG TAGTCGAAGA ATTAACAGGA CCGGTTGAAG TTAATCCTGA ATTTTTAGCT 
GCTTTACCTC CAGCAATACA AGAAGAAGTT CTTGCGCAAC AACGCTTGGA ACAACAACGT 
CATGCAGCCG CAAGTGCCAA TCCAGAAGAC CCAGTTGACG CAGCTGCATT CTTCCAAAAT 
TTACAACCTT CTCTTCGACA AGCGATTTTA ACTGATATGG AAGAATCGCA AATATCCGTT 
CTTCCACCAG ACTTAGCTCA AGAAGCTCAA AATCTTCGAA GAGAATGGGA AGCTCGAAAT 
AGACATATAA TGCAAGATCG ATTTCTTAAT CATGTTAGTC AAGGAAATGC CGCCTTATCT 
AGTATATTGC GAAATTCTGG AAGAGGTAGA GGTGGTGGAA CGCGTTATGC AATTCATACA 
GTAGCACAAA GAGGATGGCA ACCATGGTCA AATGGTGATC CTAGTATAAC GCATCATGGA 
GCGGGTCTAC GACTCAGAGG CAGACAATTA TTGGATCATG AGTCGATGGC TTGCGTATTG 
GTATTACTGT TTGTTGATGA ACCAAAAATT AATACATTAA GGCTTCATAG AGTTATCAGG 
AATCTTTGCT ATCACGGTAG CACAAGGGAA TGGGTAGTTA GAGCTTTACT AAGTATCATG 
GAAAGAAGTA ATGATGAAAA TAAAGATATA GCCATGGACT TTGGTGGAAA AATCAGAAGA 
AAAAGCTTAG TGCCCATGAT GCATCACGAT TATTGTGCTC CTAAAATTTT TGATCAAAGA 
AGCAATGCAC AGTCATGGTT AAACATCAGT ATTGATGCTG CGCTTGGATG TAGAGCAAAT 
GTTTTCCATA TTATTAGACA TGGAGGCAAA AAATCAGAAA GACAAGGCAG TAGTATTGCC 
ATCCATCCTC AAGCAGCTCC TACAGTATGC AGGCATACAT TAGAATTATT AATATCATTG 
GCTAAAGGCT TTCCTGGTTA CTTTGTACCA ATCAAGTCTA AAGAATCAGA TGAAAAAGAA 
AGTAACAAAG AAAAAGATTC AAATAACAAA GAAGAAGTCG GTTCGAAAGG TAAATCGACA 
TCGAAACCAG GAAAAAGTGA TCAACCTGAT TTTTGGGATA TGTTGTTGAA ACTTGATTCA 
TGTGCTTCGA AAAAAGGAAA ATCAGTTGCT AGATCGCACT CAAATTCAAA TTTAGGAAAT 
GATACGGAAC ATAATATCAC AACTTTTGAG TCATCCGCTT TCGGTCAATT AATTTCAATG 
CTAAACTGGT CGGTTATTAA ACGTAGCAGT CAATTAACGG ACAAAGTGTT GAAATTATTG 
TCTTTAATTT CAATCGGATT AACGGAAGTG AATCCGTATC GTAGACAAGA AGGAAATAAG 
AATAAAAGAA CAGATGTGAA TAAAGAACAA AGTATTGCTG CACCAGAAGA ACATCTTAAA 
TTGGCTGTGC AAGTATTGAC AAGTAAGAGT TGCTCTGAAG AAGGACTTGA AGATGCAACA 
GCTTTGTTGC TTAATTTATC TCATTGTCCT GATCCTACTA GACAATTGAT ATTAAAATTA 
CTCTTAGAAG GTGCCATGGA ATTGGCTAAT GTTGTTTGTG AACACATTAA AGATTTATTA 
ATGGAGTTAA AAGTTTTGAA TCGTGAACTT AGACGAAATT CTATCGAAGA GCAACCGTCA 
ACTAGTACTG GAGGTTCTCG TTGGGTTCTT TTAGATAGGT TTACCAACGA GAGTGTAGTT 
ATTACTGCGC CGAGTAAAGT TAAAGCTGGC AGCGATCTTC AATTACCATC GATGGCTGCT 
CTTGTTAGCA AAACTTCTAG TCAGGCATTT TTCTTAAGAA TACTTAAAGT GATTGTACAA 
ATAAGGGAAG CTGTACGTTT AGCAAATAAT AAAAAAACAT CCACTCAAAG TTCCGAAAGT 
TCTGTAGATA CTGAAAATAC TAATCAAGCT TCAGAATCAG GTGAAGATAA GCTTCCATTA 
TTAAGTGAAA GTTTAGTATT AGATCATCTA TGGGAAACAC TTAGTGCTTG TTTATTGGAA 
TTGGAATATA CTCCAGATCA ACATGCAGTT TTAGTATTGC AACCTGCAGT AGAAGCATTT 
TTCCTAGTAC ATTCATCATC GAGTACTTCT CGTGATCGTC ATCTAAGTAC AAGTACGGAA 
AATCGCGAAG TAGTTCCTGA TCTAACACCT GTTTCACCAA TTTATTCAGA TAATGAAGGA 
ACGTCCCAAT CAGAAGCTAC TATTAATACT TCTTGGGATA TACAACCTAC GCCACAGAAG 
ACATTACCAC CGGACCAACT CAAGTTCCTT AAATTTGCAG AAACGCACAG AACCGTTTTA 
AATCAAATTC TTCGTCAAAC AACTACGCAT TTAGCAGATG GACCATTCTC TGTGTTAGTG 
GATCACACAA GAGTTCTTGA TTTTGACGTA AAAAGACGAT ATTTCCGAAC AGAATTAGAA 
CGAATGGATG AAGGTATTCG AAGGGAAGAA TTGGCAGTAC ATGTTCGTAG GAATAATGTA 
TTTGAAGATT CATTTCGAGA ACTTCATAGG CGTAATGCTG ATGAATGGAA AAATCGTTTT 
TATATTGTTT TTGAAGGTGA AGAAGGGCAA GATGCTGGAG GATTACTTAG AGAGTGGTAC 
GTTATTATTT CCAGAGAAAT TTTTAATCCT ATGTATGCAT TGTTTACTGT AAGTCCTGGC 
GATCGAGTTA CATACATGAT AAACTCCTCT TCACATTGTA ATCCTAATCA CTTATGTTAT 
TATAAATTTG TCGGTCGAGT TATAGCTAAA GCAATTTATG ACAACAAATT ATTAGAATGT 
TACTTCACGC GTAGTTTTTA TAAACACATT TTGGGAATAC TTGTGAAACA TACAGATATG 
GAAAGTGAGG ATTATAGTTT TTACAAAGGA CTTGTATATC TCACAGAGCA TAATATTGCT 
GATCTTGGAT ACGAATTAAC TTTCTCTACA GAGGTAAACG AATTTGGTGT AAATGATGTA 
CGTGACTTAA TACCAAATGG ACGTAATATC ATTGTTACTG AGGAAACAAA ATTAGAATAT 
ATTCGGCTCG TATGTCAAAT GAAAATGACT GGAGCAATTC GTAAACAATT AAATGCGTTC 
CTAGAAGGTT TTTATGATAT TATTCCCAAA CGACTGATCT CTATATTCAA TGAACAAGAA 
CTTGAATTGT TAATTAGTGG ATTACCCAAT GTAGATATTG AGGATCTTAA AGCTAATACT 
GAATATCATA AATATAATGC AAATTCTTTA CAAATTCAAT GGTTCTGGAG AGCATTACGA 
GGTTTCGATC AAGCAGATAG GGCTAAATTT TTGCAATTTG TTACTGGAAC TTCAAAAGTG 
CCACTTCAAG GTTTCGCTGC TTTAGAAGGA ATGAATGGTA TACAAAAGTT CCAAATTCAT 
CGTGAAGATA GATCGACAGA TAGACTTCCG TCAGCACATA CATGTTTCAA TCAGCTAGAT 
CTACCAGTTT ATGAAACATA CGATAAGCTT CGCACGAATT TATTAAAAGC GATCCACGAA 
TGCAGTGAAG GATTCGGTTT CGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026297972.1
CDS	794..13240
Translation	MKIDRNRLRKYTSEAPAKCQALINKLRSCSHAELLEELKQIDAWTFGKCELFHWVDVLDLSDSILEEAAAKSPDNPWELACDLPQNREAKELLLWVLHLTTLLIEHSYSRHLYSSMEHLVTLLSSCDMHVVLGVLNILYMFSKRSNFIARLNSDKRQALLSRLTLLAESWGGKENGFGLAECCKEYPDKPLPASATTLHFEFYAENSTTDSNATSNIGTKKSGQTNFVTCIHIENVDKLGKTPAQIMNDLLKVNNVPQDRQIALFTHIRLAHSFSDYRRRLQCVHARLQALSILVYNSELEENAHSLLHPGLLEELVELLELPHAHLVELRAAALRTLTSIIHLDRNPHFPKKPSSRLNMIIDVTGANSYHGFFPVLIRTCISTLISPQPEGQPFPLSLATALFSFLYHLSSYEAGCEALVSCGMMESLLKIMNWPGSEFEHLTFVTRAVRVIDLITNIDMQGFQAHGGLASFINRLDMEVNVCRKEQPFEIEPIHYQDNSQATRERSMDRDESSTSSRAFDEREESSNPMEFSEDENMSSKTDDKNEQIPDYSAGKTGKTCLPQRVTLLKALLNFIKKIIQDPTFSDSIRHVMEGTLPSSLRHIISNSEYYGPSLFLIATDAVTVYVFQEPSLLSSLQDNGLTDLVLHALLIKEIPASREVLGSLPNVFSALCLNQRGLESFVKRRPFERLFKVLLSPLYLPAMNRRRSTDPLGDTASNLGNAMDELMRHQPSLKVDAIAAVIKLLEELCVLGCDPRYICLQAEDKFDNSQSNTTSGNRQSSGQSVGVGVGDSGVGGGGGSGSSSDEEEEDEEEASTSSHPQREEQCSSSTAQPVPPPQPREKTPIALMEYIQNVTKFVDAILSNNSTDDHCREFVAQKGLVPLLSILGLPNLPVDHSVAQTAQAVATVCKSILNLAHEPLVLKTGLSQLNEVLNLLKPLYNYMEAPGGSVLLHELVNAPNLETAFTSDTATPLLHAMNAAYGYIIMFAQVCRTSQSEIRNLAMNHWGSELGLTVLKGLSELYTALIWESTALLALTTEDFIPAGCDFGKEDMDKLIRPESTNEGESTSWSGATSTDMGSNGMTTAMEALSTDPPPIPMEVDEKPSVSTGRISPNSHRLTYPKHLLGLTSKMGRALAELFAILVKLCMGSPIRQRRGQQMPPIPALPSQASRSVATALNCVLIRGLSWDKLPPSTVPKLQVTFLMCTLSFVSPALFDEKGYPFHLMLQKFVKLGGQNAFFAAFHWVLSGGGQFPLSEGLEHPNLPDCTGEFLDGWLMILEKMVNPKTLLDSPYVVSSKCTGIYKVYETFDPIKYLTDIHKEAFEAVMLMWGKKPLKNYGVHMTELILSILRHILRSEQIIKEHAEKEENNEGGISGSTSGTSGIGRGGTISDRREEPEADVNPEHLRQLMDMGFSRAHCIEALLHTLTVEQATDYLLTNPATLRRSDVPAGDVSGQDLIMDLELVDDDQIMHAIAMSLGETAETRNVFSTEESSSREATTITESIDKFTQNALSQCLNLLDLMPDTVYRICDLLVAIAKRNGDEWRDNMLRQLMKEIGGLVDYLIEVAESQDPNAGVKLVECKEANKVTGRIYLYTMFFEGTFQEMRVPCAQIVEEYAILNKLVRLLVVSEGPLTASKSKIVKEGNKDTSSTKTRKWLAPLLLLIERLEKVAVLTKRKELMYKVTTRMWKWFDLSTGKWNLYSPVNNRIINDAYWAGESSTRVTCNRRRYVINFSCMTQVNEETGNRRPITMGFKLHTGNEDGGSGSDTNMDTDENRIEDKRNTVLQGLDPSMAPSIIRACVRLMAIPVDRDALHAAMKVCLRLTRDYENAEIFVREGGVKLLLEMSQASSFIGCITLSTLLIRHALEEPCTLRLAMEKVIRSRTQGNIPPACKEILFMTRQINSAVCRNPEVYREVCENILRVDIRLLGKEDDDNRLIVKALPPSHSSVMPMVEKASISVVKDLLNALIKPMPVVSDEKSSTPTRSPEKKTSYSNVGSSSSRRDVLRNNATTSNDTLDDEDQVSQIIPMKIYPDNSNNSKVEPEEAEKPLLTKSAILKILAEAVRSYSAVASLITEHAYRAGQSEMVPEYTTALAFLLDKLLPMSPENSSDKQCSSMARMLIAAIASCNHSPDVQTTLVTEVKAALTRTLALPESSEKHAQIQLLVGLISTMIDSCPPTSYNQLRTSLKMHQYNNVNYIVRIMLRKGIITDLAKIPHSLDLSSPNMAATINAALKPLETLSRIINQPMPGAINNKFTKPKSRTVQEEPIGEQTGTTTSEATHAVGEETNEDAENTEHDISVTAESLEPTSESQVHEDEAALEDIMDQLLERDGPGVTEDQSSRPHRPMDIDEESLAIRDGEADFDPTRDATEDLMSSDSDSDSNPSDDNEDEVQDDEDEEEEEEDDGEENEEEEEEEEEGSSNYEEDGIEFYEDVGESGVFRMIPSTDRDGSSVVMIRHNIDNQDNVNSSTTVTPRSSLPWSTTFPLPLGLFENPPSISESSGINSHSLLIPQGGLDATNTRGQRAVRPRRYQYLQLPNPRNPNPPVILQRLLGPAAQAIPLSASQVLASSFRDTRVVVMDNGVGILTNQEEEQIDFVDQSGYLFGSSLATTVNNTPTALHWWIEESKLLDGDSQTDCCVGVVHKLIPGLEKRRNAELGERRERRKMQYASEKKNEKDNKDGKDHLNNTESGSSTIISMDEQISTASTSQREETNNFVPRFETCVETTRLERTLDEDIIDVTGEMDATIQEDEERPPPPPPEEQPTEPRDPRISNRNPTLELAESIVDSVLGPCASEASRLRQSERTVEGSNTNETSSRPNTSIVVDFSQEPNEDLLRESDSSEWIRILTDDSQSNVERNANILNQVPTTSASENINPEISEQQQQQQQQQQPSIQQQQQQEPQQSQEQSQRQQQNQQSQEQQLPDGVDPSFLAALPEDMRDEVIAEQLRLQRIRQRAQATVVEELTGPVEVNPEFLAALPPAIQEEVLAQQRLEQQRHAAASANPEDPVDAAAFFQNLQPSLRQAILTDMEESQISVLPPDLAQEAQNLRREWEARNRHIMQDRFLNHVSQGNAALSSILRNSGRGRGGGTRYAIHTVAQRGWQPWSNGDPSITHHGAGLRLRGRQLLDHESMACVLVLLFVDEPKINTLRLHRVIRNLCYHGSTREWVVRALLSIMERSNDENKDIAMDFGGKIRRKSLVPMMHHDYCAPKIFDQRSNAQSWLNISIDAALGCRANVFHIIRHGGKKSERQGSSIAIHPQAAPTVCRHTLELLISLAKGFPGYFVPIKSKESDEKESNKEKDSNNKEEVGSKGKSTSKPGKSDQPDFWDMLLKLDSCASKKGKSVARSHSNSNLGNDTEHNITTFESSAFGQLISMLNWSVIKRSSQLTDKVLKLLSLISIGLTEVNPYRRQEGNKNKRTDVNKEQSIAAPEEHLKLAVQVLTSKSCSEEGLEDATALLLNLSHCPDPTRQLILKLLLEGAMELANVVCEHIKDLLMELKVLNRELRRNSIEEQPSTSTGGSRWVLLDRFTNESVVITAPSKVKAGSDLQLPSMAALVSKTSSQAFFLRILKVIVQIREAVRLANNKKTSTQSSESSVDTENTNQASESGEDKLPLLSESLVLDHLWETLSACLLELEYTPDQHAVLVLQPAVEAFFLVHSSSSTSRDRHLSTSTENREVVPDLTPVSPIYSDNEGTSQSEATINTSWDIQPTPQKTLPPDQLKFLKFAETHRTVLNQILRQTTTHLADGPFSVLVDHTRVLDFDVKRRYFRTELERMDEGIRREELAVHVRRNNVFEDSFRELHRRNADEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFTVSPGDRVTYMINSSSHCNPNHLCYYKFVGRVIAKAIYDNKLLECYFTRSFYKHILGILVKHTDMESEDYSFYKGLVYLTEHNIADLGYELTFSTEVNEFGVNDVRDLIPNGRNIIVTEETKLEYIRLVCQMKMTGAIRKQLNAFLEGFYDIIPKRLISIFNEQELELLISGLPNVDIEDLKANTEYHKYNANSLQIQWFWRALRGFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHREDRSTDRLPSAHTCFNQLDLPVYETYDKLRTNLLKAIHECSEGFGFA

Target ORF information:

RefSeq Version	XM_026442187.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera E3 ubiquitin-protein ligase HUWE1 (LOC411961), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026442187.1

ORF Insert Sequence:

ATGAAAATTG ATCGCAATAG ATTGAGAAAA TATACATCAG AAGCGCCTGC AAAATGTCAG 
GCGCTTATAA ACAAGTTGCG CTCATGCTCC CATGCAGAAT TGCTAGAGGA GCTAAAGCAA 
ATAGATGCAT GGACTTTTGG TAAATGTGAA TTATTTCATT GGGTCGATGT ATTGGACCTT 
AGTGACAGCA TTTTGGAAGA AGCTGCTGCT AAAAGCCCAG ATAATCCATG GGAATTGGCT 
TGTGATCTCC CTCAAAATAG AGAGGCAAAA GAACTTCTCC TTTGGGTTCT ACATCTTACA 
ACATTATTGA TTGAACATTC ATATTCGCGA CATTTATACA GCAGTATGGA ACATCTAGTG 
ACTTTATTAT CAAGCTGTGA CATGCATGTT GTCCTTGGTG TATTAAATAT TCTCTACATG 
TTCAGTAAAC GTAGCAATTT CATTGCACGT TTAAATAGTG ACAAGAGACA AGCTTTATTA 
AGTAGACTTA CTCTTTTAGC AGAGAGTTGG GGAGGTAAAG AAAATGGATT TGGTTTAGCA 
GAATGTTGTA AAGAATATCC AGATAAGCCA CTTCCAGCAA GTGCCACAAC ATTGCATTTC 
GAATTTTATG CAGAAAATTC AACGACAGAT TCAAATGCCA CATCAAACAT TGGCACAAAA 
AAATCTGGAC AGACGAATTT TGTAACATGT ATACATATAG AAAATGTAGA TAAACTGGGT 
AAAACACCAG CACAAATTAT GAATGATTTG TTAAAAGTCA ATAACGTACC CCAAGATCGA 
CAGATTGCGT TATTTACACA TATAAGATTA GCACACAGTT TCTCTGACTA TAGAAGACGA 
TTACAATGTG TGCACGCTCG GCTACAGGCA TTATCAATAC TTGTATATAA TAGTGAACTA 
GAAGAAAATG CACATAGTTT GTTGCATCCT GGACTTTTAG AAGAATTAGT TGAATTACTG 
GAACTTCCAC ATGCACATCT TGTTGAATTA CGTGCTGCTG CTTTAAGAAC TCTTACAAGT 
ATCATTCATT TAGATCGCAA TCCACATTTT CCAAAAAAAC CTAGTTCAAG ATTAAATATG 
ATAATCGATG TCACTGGAGC CAATTCATAT CATGGATTTT TTCCTGTATT AATTCGAACT 
TGCATTTCAA CATTAATTTC TCCACAACCT GAAGGACAAC CATTTCCTTT GTCACTTGCA 
ACAGCTTTAT TTAGCTTTCT TTACCATCTT TCAAGTTATG AAGCAGGATG TGAAGCACTT 
GTTAGTTGTG GGATGATGGA AAGTTTGTTA AAAATCATGA ATTGGCCTGG TAGCGAGTTT 
GAACATTTGA CGTTTGTAAC AAGGGCAGTT CGTGTTATAG ACTTAATAAC AAATATAGAT 
ATGCAAGGAT TTCAAGCTCA TGGTGGTTTA GCCAGTTTTA TTAATCGTCT TGATATGGAA 
GTAAATGTTT GTAGAAAAGA ACAACCATTT GAAATAGAAC CAATACATTA TCAAGATAAT 
TCTCAGGCCA CTCGTGAAAG ATCAATGGAT CGCGATGAAA GTTCTACGTC TTCACGTGCT 
TTTGATGAGC GTGAAGAATC ATCTAATCCT ATGGAATTTT CGGAAGATGA GAACATGAGT 
TCCAAAACCG ATGATAAAAA TGAACAAATA CCAGATTATT CAGCTGGAAA AACTGGAAAA 
ACTTGTCTAC CACAGCGAGT TACACTTTTA AAAGCGTTAT TAAATTTTAT TAAAAAAATT 
ATTCAAGATC CTACATTTTC TGATAGTATT AGACATGTTA TGGAGGGTAC TTTACCTTCT 
TCCTTGAGAC ATATTATCAG TAATTCAGAA TATTATGGTC CTTCTTTGTT CCTTATTGCA 
ACAGATGCTG TAACAGTATA TGTTTTCCAA GAGCCATCTT TATTATCTTC TTTACAGGAT 
AATGGATTAA CTGATCTTGT ATTGCATGCT TTACTAATTA AAGAAATTCC TGCTAGTAGA 
GAAGTTCTAG GATCATTGCC TAATGTATTT AGTGCTTTAT GTTTAAATCA GCGTGGACTA 
GAATCATTTG TAAAACGACG TCCTTTCGAA CGCTTATTTA AAGTATTATT ATCGCCACTT 
TATCTGCCTG CAATGAATCG ACGTCGTAGC ACAGATCCTT TAGGTGATAC TGCTTCGAAT 
TTGGGTAATG CAATGGATGA ATTAATGCGA CATCAGCCAA GTTTGAAGGT TGATGCAATC 
GCTGCTGTTA TAAAGTTATT AGAAGAACTT TGTGTACTGG GTTGTGATCC TCGTTATATA 
TGCTTGCAAG CTGAAGATAA ATTCGATAAT TCTCAATCAA ATACTACTTC TGGAAATCGT 
CAATCTTCTG GACAATCTGT TGGAGTTGGA GTTGGAGATT CTGGTGTAGG AGGAGGAGGT 
GGTAGTGGAA GTAGTAGCGA CGAAGAAGAA GAAGATGAAG AAGAAGCTAG TACAAGTTCT 
CATCCACAAC GTGAAGAACA ATGTTCATCA TCCACTGCTC AACCAGTTCC ACCACCTCAA 
CCTAGAGAAA AAACTCCAAT AGCTTTAATG GAATATATAC AAAATGTTAC AAAATTCGTT 
GATGCTATTC TCAGTAATAA TTCCACGGAT GATCATTGTA GAGAATTTGT CGCCCAAAAA 
GGTCTCGTAC CATTATTGTC AATTTTAGGA TTACCTAATC TTCCAGTGGA TCATTCAGTT 
GCCCAAACGG CACAAGCTGT AGCTACAGTT TGTAAAAGTA TTTTAAATCT CGCACATGAA 
CCATTAGTTC TTAAAACTGG TCTATCTCAG TTGAATGAAG TTTTGAATTT GCTGAAACCA 
TTATACAATT ATATGGAAGC TCCAGGAGGA TCTGTTTTAT TACATGAACT TGTCAATGCT 
CCAAATCTGG AAACAGCTTT TACCAGTGAT ACGGCAACAC CTTTACTTCA TGCTATGAAT 
GCTGCTTATG GATATATAAT AATGTTTGCA CAAGTGTGCC GTACCAGTCA AAGTGAAATC 
AGAAACTTGG CTATGAATCA TTGGGGTTCT GAACTTGGTT TGACTGTTCT GAAAGGTTTA 
TCAGAATTAT ATACAGCTCT AATCTGGGAA AGTACAGCCT TATTAGCACT TACCACGGAA 
GATTTTATTC CGGCTGGTTG TGATTTTGGA AAAGAAGATA TGGATAAACT TATACGTCCT 
GAATCAACAA ATGAAGGTGA AAGTACTAGT TGGTCTGGTG CTACATCTAC AGATATGGGA 
AGTAATGGGA TGACTACAGC TATGGAAGCT TTAAGCACTG ATCCGCCTCC TATACCTATG 
GAAGTAGACG AAAAACCAAG TGTTAGTACA GGAAGAATAT CTCCAAATTC TCATCGACTT 
ACATATCCTA AACATTTGCT GGGACTTACA TCTAAAATGG GCAGAGCTTT AGCAGAACTC 
TTTGCTATAC TTGTAAAGCT TTGCATGGGC TCTCCAATTA GACAACGCAG AGGACAACAG 
ATGCCTCCAA TACCAGCCTT ACCTTCACAA GCATCTAGAA GTGTAGCAAC TGCTTTAAAT 
TGTGTATTAA TTCGCGGATT ATCATGGGAT AAATTACCAC CATCGACAGT GCCAAAACTT 
CAAGTAACCT TTTTGATGTG CACTTTAAGT TTTGTATCTC CTGCATTATT TGATGAAAAG 
GGGTATCCAT TTCATTTGAT GCTTCAAAAG TTCGTTAAAC TCGGCGGACA AAATGCATTT 
TTCGCTGCTT TTCATTGGGT ATTATCTGGA GGAGGACAGT TTCCGTTATC TGAAGGATTA 
GAGCATCCAA ATTTACCTGA TTGTACTGGA GAATTCTTAG ATGGTTGGTT GATGATTTTA 
GAAAAAATGG TGAATCCTAA AACTCTTCTT GATTCTCCAT ATGTTGTTTC TTCAAAATGT 
ACTGGAATAT ATAAAGTATA TGAAACATTT GATCCTATAA AATATCTTAC GGATATTCAT 
AAGGAAGCTT TCGAGGCAGT GATGCTAATG TGGGGAAAGA AACCTTTAAA AAATTATGGA 
GTTCATATGA CAGAACTTAT TTTATCTATC TTAAGACATA TTTTACGAAG TGAGCAGATC 
ATAAAGGAAC ATGCTGAAAA AGAAGAAAAT AATGAAGGAG GTATTAGTGG TAGTACTAGT 
GGAACTAGTG GAATCGGAAG AGGTGGAACA ATATCCGATC GCAGAGAAGA ACCAGAAGCA 
GATGTAAATC CAGAACATCT AAGACAGTTG ATGGATATGG GTTTCAGTAG AGCTCATTGC 
ATAGAAGCTT TGCTTCATAC ATTGACAGTT GAACAAGCTA CTGATTATTT GTTAACTAAT 
CCTGCTACTC TACGTCGATC AGATGTGCCA GCAGGTGATG TAAGCGGACA AGATTTAATA 
ATGGATTTAG AATTGGTAGA TGATGATCAA ATAATGCATG CCATAGCGAT GTCGCTTGGA 
GAAACGGCGG AAACGCGAAA TGTTTTCAGC ACAGAAGAAT CCAGTTCACG TGAGGCTACT 
ACCATAACTG AAAGCATAGA TAAATTTACA CAAAATGCTT TAAGTCAATG TTTAAATTTA 
TTGGATCTCA TGCCTGATAC TGTATACAGA ATTTGCGATT TATTAGTTGC CATCGCTAAA 
AGAAACGGAG ATGAATGGCG TGATAATATG TTGCGACAAC TTATGAAAGA AATTGGAGGT 
TTGGTAGATT ATTTAATCGA AGTTGCTGAA AGTCAAGACC CGAATGCTGG AGTAAAATTA 
GTAGAATGTA AAGAAGCTAA TAAAGTTACT GGACGTATTT ATCTGTATAC AATGTTCTTT 
GAAGGAACAT TCCAAGAAAT GCGAGTGCCC TGTGCTCAAA TTGTAGAAGA ATATGCTATT 
TTAAATAAAC TTGTACGTTT ATTAGTCGTA AGTGAGGGTC CGTTAACTGC AAGTAAAAGT 
AAAATTGTTA AGGAAGGTAA TAAGGATACA TCATCTACGA AAACTAGAAA ATGGCTTGCA 
CCTTTGTTAT TGCTGATAGA ACGTTTAGAA AAAGTAGCGG TATTGACAAA GAGAAAAGAA 
TTAATGTATA AAGTAACAAC CAGGATGTGG AAATGGTTCG ATCTCAGCAC AGGAAAATGG 
AATTTATATT CTCCAGTAAA TAATCGAATA ATTAATGATG CGTATTGGGC TGGCGAGTCT 
AGTACGAGAG TAACATGTAA CAGGCGACGA TATGTAATTA ATTTTTCATG CATGACTCAA 
GTTAATGAAG AAACTGGTAA TAGAAGACCT ATTACGATGG GCTTTAAATT GCATACCGGA 
AATGAAGATG GTGGATCAGG TTCTGATACA AATATGGACA CTGACGAAAA TCGAATAGAA 
GATAAACGGA ATACAGTTTT GCAAGGGTTA GATCCTAGCA TGGCTCCAAG TATCATACGC 
GCTTGTGTAA GATTGATGGC TATTCCAGTA GATCGAGATG CTTTACATGC AGCTATGAAA 
GTTTGTTTGA GACTTACAAG AGATTATGAA AATGCCGAAA TATTTGTTCG TGAGGGTGGT 
GTGAAACTTC TTCTAGAAAT GTCTCAAGCA AGTAGTTTCA TTGGATGCAT AACTTTAAGT 
ACTCTTTTAA TAAGGCATGC TTTGGAAGAA CCATGTACTC TTCGATTAGC CATGGAAAAA 
GTAATTCGTA GTCGTACTCA AGGCAATATT CCTCCAGCTT GCAAAGAAAT CTTATTTATG 
ACACGTCAGA TTAATAGCGC TGTATGTCGC AATCCGGAAG TTTATAGAGA AGTTTGTGAA 
AACATTTTAA GAGTAGATAT TCGTCTATTA GGAAAAGAGG ATGATGATAA TAGATTAATT 
GTCAAGGCAC TTCCACCAAG TCACTCATCA GTAATGCCTA TGGTAGAGAA AGCTTCAATA 
TCTGTTGTAA AAGATCTTCT GAATGCTCTT ATAAAGCCTA TGCCAGTTGT CTCTGATGAA 
AAGTCTTCTA CACCAACAAG GAGTCCAGAA AAGAAGACAT CTTATTCTAA TGTTGGAAGT 
TCATCTTCTC GACGAGATGT TCTTAGAAAT AATGCAACAA CATCAAATGA TACTCTTGAT 
GATGAGGATC AAGTTTCTCA AATAATACCA ATGAAAATTT ATCCAGATAA TAGCAATAAC 
AGTAAAGTGG AACCAGAAGA AGCAGAAAAA CCTTTATTAA CAAAATCTGC TATTTTGAAG 
ATACTTGCAG AAGCCGTTCG CTCATATAGT GCAGTCGCAA GTTTAATTAC AGAGCATGCA 
TATCGAGCCG GACAAAGTGA AATGGTTCCT GAATACACAA CAGCTCTTGC TTTTCTTTTA 
GATAAGCTTT TACCAATGTC ACCAGAAAAT TCAAGTGACA AACAATGCAG CAGTATGGCG 
AGAATGTTAA TAGCTGCTAT TGCTTCCTGT AATCATTCTC CGGATGTTCA AACAACTTTG 
GTGACTGAAG TAAAAGCAGC TTTAACAAGA ACTTTAGCTC TTCCTGAAAG TTCTGAGAAA 
CATGCACAAA TTCAATTATT AGTTGGATTG ATCTCTACTA TGATTGATAG TTGTCCACCA 
ACTTCTTACA ATCAATTGAG GACTTCCTTG AAGATGCATC AATACAATAA TGTTAACTAT 
ATTGTAAGAA TCATGTTGAG AAAAGGAATA ATTACAGATT TAGCAAAAAT TCCACATAGT 
CTGGATTTAT CAAGTCCAAA TATGGCTGCT ACCATAAATG CTGCTTTAAA ACCATTGGAA 
ACGCTTTCGC GAATTATAAA TCAACCAATG CCAGGTGCTA TTAATAACAA ATTTACAAAG 
CCAAAAAGTA GAACAGTTCA AGAGGAACCT ATTGGAGAAC AAACAGGAAC TACAACATCA 
GAAGCGACAC ATGCTGTTGG CGAAGAAACG AACGAAGACG CAGAGAATAC AGAACACGAT 
ATATCTGTTA CTGCGGAAAG TCTTGAACCT ACTTCAGAAA GTCAAGTACA TGAAGATGAA 
GCTGCTTTAG AAGACATTAT GGATCAACTG CTTGAAAGGG ACGGGCCGGG CGTGACGGAA 
GATCAATCTT CTCGACCACA TAGACCAATG GACATTGATG AAGAAAGCCT TGCAATAAGA 
GATGGAGAAG CTGATTTTGA TCCAACACGA GATGCTACGG AGGATTTAAT GAGCTCTGAT 
TCGGATTCTG ATAGCAATCC ATCTGATGAT AATGAAGATG AAGTGCAAGA TGATGAAGAT 
GAAGAAGAAG AAGAAGAGGA TGATGGAGAA GAAAATGAAG AAGAAGAGGA AGAAGAAGAA 
GAAGGATCCT CAAATTACGA AGAAGACGGA ATTGAATTCT ATGAAGATGT AGGAGAAAGT 
GGTGTATTCA GAATGATTCC ATCAACAGAT CGTGATGGTA GTAGTGTCGT AATGATTCGA 
CATAATATAG ATAATCAAGA CAATGTGAAT TCTTCAACTA CAGTTACACC ACGATCTAGT 
CTTCCATGGA GTACAACATT TCCTCTTCCT TTGGGACTAT TTGAAAATCC TCCATCAATT 
TCTGAAAGTA GTGGTATTAA TTCCCATTCG TTGCTAATAC CACAGGGAGG TTTAGATGCT 
ACTAATACAA GAGGTCAGAG AGCTGTACGA CCGCGAAGAT ATCAATATCT TCAATTACCA 
AATCCACGAA ATCCAAATCC ACCTGTAATA TTGCAAAGAC TTTTAGGTCC AGCTGCACAA 
GCAATTCCAT TATCTGCCAG TCAGGTTTTG GCTTCCAGTT TTCGAGATAC ACGCGTTGTA 
GTAATGGATA ATGGTGTTGG AATATTAACA AATCAAGAAG AAGAACAAAT AGATTTTGTG 
GATCAATCTG GCTATCTTTT CGGATCTAGT CTTGCAACCA CAGTAAATAA TACTCCTACT 
GCTTTACATT GGTGGATTGA AGAAAGCAAA CTATTAGATG GAGATTCCCA AACGGATTGC 
TGTGTTGGTG TTGTACATAA ACTAATTCCT GGATTGGAAA AACGTAGAAA TGCAGAATTG 
GGAGAACGAC GTGAAAGACG TAAAATGCAA TACGCTTCTG AGAAAAAAAA TGAAAAAGAT 
AACAAAGATG GAAAAGATCA TTTAAATAAT ACAGAATCTG GTTCTTCTAC TATAATATCT 
ATGGATGAAC AAATATCAAC TGCATCAACT TCACAAAGAG AAGAAACGAA TAATTTTGTT 
CCTAGATTTG AAACTTGCGT GGAAACAACT CGATTAGAAC GCACACTTGA CGAAGATATA 
ATTGATGTAA CTGGTGAGAT GGATGCAACC ATTCAAGAAG ATGAAGAACG ACCACCACCT 
CCCCCACCAG AAGAACAACC AACAGAACCT AGAGATCCTA GAATAAGCAA TCGAAATCCA 
ACTTTGGAAC TTGCTGAAAG TATAGTGGAT TCTGTACTTG GACCTTGCGC GTCTGAAGCA 
AGCAGATTAA GACAATCTGA ACGTACCGTT GAAGGATCTA ATACTAATGA AACATCTAGT 
CGACCCAATA CGTCCATAGT TGTTGATTTC AGTCAAGAAC CTAATGAAGA TCTATTAAGA 
GAAAGTGATT CATCTGAATG GATAAGAATT CTTACGGATG ACAGTCAATC AAATGTTGAA 
CGAAATGCGA ATATTCTCAA TCAAGTACCT ACCACTTCCG CATCAGAAAA TATTAATCCA 
GAAATATCAG AACAACAACA GCAACAGCAG CAGCAACAAC AACCATCTAT ACAACAGCAG 
CAACAACAAG AGCCACAACA ATCACAAGAA CAGTCGCAAC GACAACAACA AAACCAACAA 
TCTCAAGAAC AACAATTACC GGATGGTGTA GATCCTTCTT TCTTAGCTGC ATTACCCGAA 
GATATGCGTG ACGAAGTTAT TGCTGAGCAA CTAAGACTTC AACGAATTCG GCAAAGAGCA 
CAGGCAACTG TAGTCGAAGA ATTAACAGGA CCGGTTGAAG TTAATCCTGA ATTTTTAGCT 
GCTTTACCTC CAGCAATACA AGAAGAAGTT CTTGCGCAAC AACGCTTGGA ACAACAACGT 
CATGCAGCCG CAAGTGCCAA TCCAGAAGAC CCAGTTGACG CAGCTGCATT CTTCCAAAAT 
TTACAACCTT CTCTTCGACA AGCGATTTTA ACTGATATGG AAGAATCGCA AATATCCGTT 
CTTCCACCAG ACTTAGCTCA AGAAGCTCAA AATCTTCGAA GAGAATGGGA AGCTCGAAAT 
AGACATATAA TGCAAGATCG ATTTCTTAAT CATGTTAGTC AAGGAAATGC CGCCTTATCT 
AGTATATTGC GAAATTCTGG AAGAGGTAGA GGTGGTGGAA CGCGTTATGC AATTCATACA 
GTAGCACAAA GAGGATGGCA ACCATGGTCA AATGGTGATC CTAGTATAAC GCATCATGGA 
GCGGGTCTAC GACTCAGAGG CAGACAATTA TTGGATCATG AGTCGATGGC TTGCGTATTG 
GTATTACTGT TTGTTGATGA ACCAAAAATT AATACATTAA GGCTTCATAG AGTTATCAGG 
AATCTTTGCT ATCACGGTAG CACAAGGGAA TGGGTAGTTA GAGCTTTACT AAGTATCATG 
GAAAGAAGTA ATGATGAAAA TAAAGATATA GCCATGGACT TTGGTGGAAA AATCAGAAGA 
AAAAGCTTAG TGCCCATGAT GCATCACGAT TATTGTGCTC CTAAAATTTT TGATCAAAGA 
AGCAATGCAC AGTCATGGTT AAACATCAGT ATTGATGCTG CGCTTGGATG TAGAGCAAAT 
GTTTTCCATA TTATTAGACA TGGAGGCAAA AAATCAGAAA GACAAGGCAG TAGTATTGCC 
ATCCATCCTC AAGCAGCTCC TACAGTATGC AGGCATACAT TAGAATTATT AATATCATTG 
GCTAAAGGCT TTCCTGGTTA CTTTGTACCA ATCAAGTCTA AAGAATCAGA TGAAAAAGAA 
AGTAACAAAG AAAAAGATTC AAATAACAAA GAAGAAGTCG GTTCGAAAGG TAAATCGACA 
TCGAAACCAG GAAAAAGTGA TCAACCTGAT TTTTGGGATA TGTTGTTGAA ACTTGATTCA 
TGTGCTTCGA AAAAAGGAAA ATCAGTTGCT AGATCGCACT CAAATTCAAA TTTAGGAAAT 
GATACGGAAC ATAATATCAC AACTTTTGAG TCATCCGCTT TCGGTCAATT AATTTCAATG 
CTAAACTGGT CGGTTATTAA ACGTAGCAGT CAATTAACGG ACAAAGTGTT GAAATTATTG 
TCTTTAATTT CAATCGGATT AACGGAAGTG AATCCGTATC GTAGACAAGA AGGAAATAAG 
AATAAAAGAA CAGATGTGAA TAAAGAACAA AGTATTGCTG CACCAGAAGA ACATCTTAAA 
TTGGCTGTGC AAGTATTGAC AAGTAAGAGT TGCTCTGAAG AAGGACTTGA AGATGCAACA 
GCTTTGTTGC TTAATTTATC TCATTGTCCT GATCCTACTA GACAATTGAT ATTAAAATTA 
CTCTTAGAAG GTGCCATGGA ATTGGCTAAT GTTGTTTGTG AACACATTAA AGATTTATTA 
ATGGAGTTAA AAGTTTTGAA TCGTGAACTT AGACGAAATT CTATCGAAGA GCAACCGTCA 
ACTAGTACTG GAGGTTCTCG TTGGGTTCTT TTAGATAGGT TTACCAACGA GAGTGTAGTT 
ATTACTGCGC CGAGTAAAGT TAAAGCTGGC AGCGATCTTC AATTACCATC GATGGCTGCT 
CTTGTTAGCA AAACTTCTAG TCAGGCATTT TTCTTAAGAA TACTTAAAGT GATTGTACAA 
ATAAGGGAAG CTGTACGTTT AGCAAATAAT AAAAAAACAT CCACTCAAAG TTCCGAAAGT 
TCTGTAGATA CTGAAAATAC TAATCAAGCT TCAGAATCAG GTGAAGATAA GCTTCCATTA 
TTAAGTGAAA GTTTAGTATT AGATCATCTA TGGGAAACAC TTAGTGCTTG TTTATTGGAA 
TTGGAATATA CTCCAGATCA ACATGCAGTT TTAGTATTGC AACCTGCAGT AGAAGCATTT 
TTCCTAGTAC ATTCATCATC GAGTACTTCT CGTGATCGTC ATCTAAGTAC AAGTACGGAA 
AATCGCGAAG TAGTTCCTGA TCTAACACCT GTTTCACCAA TTTATTCAGA TAATGAAGGA 
ACGTCCCAAT CAGAAGCTAC TATTAATACT TCTTGGGATA TACAACCTAC GCCACAGAAG 
ACATTACCAC CGGACCAACT CAAGTTCCTT AAATTTGCAG AAACGCACAG AACCGTTTTA 
AATCAAATTC TTCGTCAAAC AACTACGCAT TTAGCAGATG GACCATTCTC TGTGTTAGTG 
GATCACACAA GAGTTCTTGA TTTTGACGTA AAAAGACGAT ATTTCCGAAC AGAATTAGAA 
CGAATGGATG AAGGTATTCG AAGGGAAGAA TTGGCAGTAC ATGTTCGTAG GAATAATGTA 
TTTGAAGATT CATTTCGAGA ACTTCATAGG CGTAATGCTG ATGAATGGAA AAATCGTTTT 
TATATTGTTT TTGAAGGTGA AGAAGGGCAA GATGCTGGAG GATTACTTAG AGAGTGGTAC 
GTTATTATTT CCAGAGAAAT TTTTAATCCT ATGTATGCAT TGTTTACTGT AAGTCCTGGC 
GATCGAGTTA CATACATGAT AAACTCCTCT TCACATTGTA ATCCTAATCA CTTATGTTAT 
TATAAATTTG TCGGTCGAGT TATAGCTAAA GCAATTTATG ACAACAAATT ATTAGAATGT 
TACTTCACGC GTAGTTTTTA TAAACACATT TTGGGAATAC TTGTGAAACA TACAGATATG 
GAAAGTGAGG ATTATAGTTT TTACAAAGGA CTTGTATATC TCACAGAGCA TAATATTGCT 
GATCTTGGAT ACGAATTAAC TTTCTCTACA GAGGTAAACG AATTTGGTGT AAATGATGTA 
CGTGACTTAA TACCAAATGG ACGTAATATC ATTGTTACTG AGGAAACAAA ATTAGAATAT 
ATTCGGCTCG TATGTCAAAT GAAAATGACT GGAGCAATTC GTAAACAATT AAATGCGTTC 
CTAGAAGGTT TTTATGATAT TATTCCCAAA CGACTGATCT CTATATTCAA TGAACAAGAA 
CTTGAATTGT TAATTAGTGG ATTACCCAAT GTAGATATTG AGGATCTTAA AGCTAATACT 
GAATATCATA AATATAATGC AAATTCTTTA CAAATTCAAT GGTTCTGGAG AGCATTACGA 
GGTTTCGATC AAGCAGATAG GGCTAAATTT TTGCAATTTG TTACTGGAAC TTCAAAAGTG 
CCACTTCAAG GTTTCGCTGC TTTAGAAGGA ATGAATGGTA TACAAAAGTT CCAAATTCAT 
CGTGAAGATA GATCGACAGA TAGACTTCCG TCAGCACATA CATGTTTCAA TCAGCTAGAT 
CTACCAGTTT ATGAAACATA CGATAAGCTT CGCACGAATT TATTAAAAGC GATCCACGAA 
TGCAGTGAAG GATTCGGTTT CGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC411961 cDNA ORF clone, Apis mellifera(honey bee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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