LOC411488 cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC411488
Entrez Gene ID	411488
Full Name	glycogen debranching enzyme
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006561204.2	XP_006561267.1	glycogen debranching enzyme isoform X2
XM_016915601.1	XP_016771090.1	glycogen debranching enzyme isoform X2
XM_006561201.2	XP_006561264.1	glycogen debranching enzyme isoform X1
XM_016915600.1	XP_016771089.1	glycogen debranching enzyme isoform X1
XM_006561203.2	XP_006561266.1	glycogen debranching enzyme isoform X2
XM_016915600.2	XP_016771089.2	LOW QUALITY PROTEIN: glycogen debranching enzyme

Pathways from BioSystems
Gene Ontology

The following LOC411488 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC411488 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHd13284	XM_006561201.2 Latest version!	Apis mellifera glycogen debranching enzyme (LOC411488), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHd13284	XM_016915600.1 Latest version!	Apis mellifera glycogen debranching enzyme (LOC411488), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHd13286	XM_006561204.2 Latest version!	Apis mellifera glycogen debranching enzyme (LOC411488), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHd13286	XM_006561203.2 Latest version!	Apis mellifera glycogen debranching enzyme (LOC411488), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHd13286	XM_016915601.1 Latest version!	Apis mellifera glycogen debranching enzyme (LOC411488), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHd26024	XM_016915600.2 Latest version!	Apis mellifera glycogen debranching enzyme (LOC411488), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC411488 ( XM_006561201.2 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_006561264.1 Apis mellifera glycogen debranching enzyme (LOC411488), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHd13284
Clone ID Related Accession (Same CDS sequence)	XM_006561201.2 , XM_016915600.1
Accession Version	XM_006561201.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1463932800000
Organism	Apis mellifera(honey bee)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007081.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 24, 2016 this sequence version replaced XM_006561201.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis mellifera Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA TACTTACAAT AAGCTATTTC TTTTCTGATG CAACAAGGAA ATTGATAAGG 
AAACTTAGGA CTATTTTCCA ATGCCTTTAC AAATATCATT ATTTAAAGTG CCTTTATTAT 
TATGCAGTGG TTAAAAATAT GTCAGAACAC ATGTCATCAA CTGAAAGTCA CGAATTTGAA 
CAAATAAGAG TTTTAACTCT AAATAATGGA GAACATCTTG ATGGTATATT ATATAGATTA 
AAGAAAGGAT GGAGAGTAGA ATTTCGTTTA GGCGCTTCTC TTCTTGGAAG AACTGTTGAT 
CTTTTTATAA ATTATCCTTT AGGAGAGAAT AAAAAATTTG AAAGACATAC ATATTATCTC 
TTAGAATGGA TTAATGAAAC AGCTAATATA TGTTTATCTC TAGCTGGTTC ATTTCATTAT 
TATCTTATAG ATCCTAATTC TGAAGGTATG AAGCCTATTG CTTCTGGATA TCTCCTTGTA 
GATCCAGAAC TTAAAATAGG GGAACATGGG AATGATTTAC CATTAGATTG TATTCAATGT 
CAAACTGTTC TTGCAAAATG TTTAGGATCA TTTTCTACCT GGGAAGATAA ATTACTAGTC 
GCTAAAAATT CTGGATATAA CATGATTCAT TTTACACCAA TTCAGGAATT GGGATATTCT 
AAATCTTCTT ATAGTCTTAG TGATCAATTA AAATTAAATC CTTCTTTTAA TGATGATAAT 
AAATCAATTA CATATGATGA TATTGAAAAA TTAATTAATA AAATGCGCAA TGAATGGAAT 
ATGTTAAGCA TCTGTGATAT TGTTTTGAAT CATACAGCAA ATGAAAGTCC TTTTTTGGTA 
TCTCATCCAG AATGTACATA TAATTGTTTG AATAGTCCTC ATTTACGTCC TGCATATATT 
TTGGATGCTG CATTATTTGA ATTAACAGTG CAAGTGGCTG CTGGAGAATG GGAATTTAAA 
GGCATCCCTT TGATAGTTGA AACTGAAGAA CATTTAAATT CAATTCGTCA TGCACTTCAC 
ACATATTTTT TGCCGCTTAT AAAAATACAC GAATTATATA TAATAGATAT TAATGAAACT 
ATTGCTGAGT TTTTGAATTT AGCACGAAAT CAAATGCCTC AAGACATCAT TGAATCATCA 
AAAACATCAG AAGATATTAA AATAATACAA GATTCAAAAT TTCGCAGATT AAAAGCAACT 
ATAAATATGC AACTTGCTTT ACAAAAATAT AACATATATA GAACTGATTG TTTTGATGAA 
GAGACTCGTT TGAAACGTTG TGCTGAAGAT TTAAGAAAAA AATTGCAGGA ACTTAATAAT 
ATAATCATTA ATGAAATACA AAATCATTTA AATGCTGCAG TTGAAAATAC AATTGCTGGT 
ATAAGATATT TTAGAGTGCA AGTTGATGGA CCTAAACTTA AAGAAATAAG TGAAAGAAAT 
CCACTAGTTC CTAGATATTT TACTGATTAT GGAACACCTA AAACATTAAC TGAAAGAGAA 
GCAACAATGT ATTCAGATGC AGGATGTTAT CTTATGGCTC ATAATGGATG GGTAATGAAT 
GGCGATCCTT TAAAAAATTT TGCAGATTCT GATTCTAATG TTTATATAAG AAGAGAACTT 
ATTGCTTGGG GTGATAGTGT AAAATTGAGA TATGGAGATA AACCAGAAGA TTGTCCTTTT 
CTATGGCAGC ATATTAGTAC ATATGTTGAA CAAACAGCTA AAATTTTTGA TGGTATTCGA 
TTAGACAATT GTCATTCAAC TCCTATTCCA GTTGCAGAGT ATATGCTTGA TGTTGCTCGT 
CGTATCCGTC CAGATTTGTA CGTTGTTGCA GAATTATTTA CAAATTCAGA TCAAAAAGAT 
AATATATTTG TTAATCGTCT TGGTATCACT TCTTTAATTC GAGAAGCTAT GTCTGCATGG 
GATAGCCACG AAGAAGGTCG ATTGGTATAT CGATATGGAG GTGAACCAGT CGGTGCATTT 
TTGCAATCGC GTAAACGACC TTTAGTACCA AGCATAGCAC ATGCCCTTTT TTTAGATTTA 
ACTCATGATA ATCCTAGCAC TGTGGAAAAA CGCAGCGTAT TCGATTTACT TCCAAGTGCT 
GCTCTTGTAT CAATGGCAGC ATGTGCTAGT GGTAGTAATC GTGGATATGA TGAATTAGTT 
CCTCATCATA TACATGTTGT AGATGAAACA AGACAATATT CATTTTGGAC AGATGATGAA 
TGTTTGATAG ATGGTATCAA ATTTGTAGGT TTTAAAACTG GTATAATTGC TGCAAAGAAA 
GCATTAAATG ATTTGCATTA TATTCTTGGA CAACAAAAAT TTTCTCAAGT ATTTGTTGAT 
CAAATGGATA ATGATATAGT AGCTGTAACA AGACACTCTC CAACAACTCA TGATAGTGTA 
ATNTTAATTG CTTTTACTGC TTTTAAACAT CCTGATCCTA ATGCTAATGA TTTGAGAAGA 
CATGTGAAAC CATTACGAGT GGAAGGTGTA ATGGAAGAAA TTATTTTGGA AGCATCCTTA 
TGTCATGTAG AAGCAAAAAA TGATATCTCA CCTTTCCATT ATTCTGAAAA ATATGTACGA 
GATGAAAGTT ATATAAATGG TCTCTCAGAA TATATTGTAA ATCTTAAAGA ACATATACAA 
ATTTGTGATT CAGAAATTTT GGAAAAAGTT GATTCTGGTG ATCCTAAAAT AACTCAACTT 
AATTTTGTAA ATTTTCAACC AGGTTCTGTT ATTGCTATTC GTGTATCTCT TCATGCTAAT 
ATAAAACCAG CTCTTGCAAA ACTTCATAAT ACTATTTCAA TAATCACATC CCAAAAAAGT 
TCAGACATAC GCGTTATTGC TTCACGTATG GATTTGATAG ATTTAAATAA AGCTTTATAT 
CGATGTGATC AGGAAGAACG CGATGAAACT TCAAATAGAT TTGATGTATA TAATATTCCA 
GGTTATGGAC CACTTGTTTA TGCAGGATTG CAAGGTATTA TTTCTTTATT AGCGGATATT 
CGTCCTAATA ATGATTTAGG TCATCCAATG TGTATTAATC TCAGACAAGG TAATTGGTTA 
ATAGATTATA CTTGGCAACG GTTAAAAGAA GACGATGGAA CAAAAGCACT TGGAGAATGG 
CTTGAAGAAG CTACTGAACC ATTTAAATTA ATACCTCGTT ATTTAGTTCC TAGTTATTTT 
GATGTTATAG TTGTAAACGT TTATATGATA CTTCTCGAAC AATGCTATAA TCTAATGTCA 
AGCTTTGTGA AAGATGGTTC TACTTTTGTC AAGATCTTAT CTTTGGTTTC AATACAAATG 
GGAGGAGTAA TAAAATCAGC TCCATTACCA GATTTGTCAC CAAATATAGA TTCTCCTAAA 
CCAAAGATTA AACAATATAA TGACGAAAGT GAACAAATAT GTATGACATT ATCTGCTGGT 
TTACCGCATT TCACAACAGG ATATATGAGA AATTGGGGAA GAGATACATT TATTGCTTTA 
AGAGGACTAT TGATATTAAC TGGCAGACAT ACAGAAGCAC GATTTATTAT TCTTGGTTAT 
GCTGGAACTT TACGACATGG TCTTATACCA AATTTACTTG ATAAAGGAAA GAATGCTAGA 
TATAACTGTC GAGATGCTTT ATGGTGGTGG TTGTATACAA TTCAATGTTA TATACAAGAA 
GTGCCAAATG GTTTAAAAAT TTTAACAGAT AAAGTTTCAA GACTGTTTCC AACTGATGAT 
TCTCCTCCAT TACCCGCGGG ACAAGTAGAT CAATCATTAC ATGATGTTAT TCAAGAAGCT 
CTTTTAGTAC ATTTTCAAGG ACTTTGTTTT AGAGAAAGAA ATGCTGGAAA ACAGATTGAT 
GAACATATGA CAGATCGTGG TTTTAATAAT CAAATTGGAG TACATCCAGA AACAGGATTT 
ATATTTGGTG GAAATGATGC AAATTGTGGT ACGTGGATGG ATAAAATGGG ATCTTCTGAA 
AAAGCTGGTA ACAAAGGAAA ACCAGCTACT CCAAGGGATG GTTCAGCTGT AGAAATAGTG 
GGTCTCAGTA AAAGTATTCT TAGTTTTTTA GCTGAATTAT ATAAACAAAA TCTTTTTCCT 
TATGGTAGTG TGCAAAGAAA AAATCGTGAT GGTTCTGTTG TAACATGGAG TTATAAACAA 
TGGGCAGATA AAATTTCATT AAATTTTGAA AAATATTTTT ATATTAATGA AATAGCAAAA 
GAAGGAGAAT TAAATTTAGA ACTTATTCAT CGGCGTGGAA TTTTTAAAGA TAGTCATGGA 
GCAACACAAG CATGGGCTGA TTATCAACTA CGACCTAATT TTCCAGTTGC AATGGTTGTT 
GCTCCAGAGT TATTTAATCC AGAACATGCA TGGACAGCAT TAAAAAAAGT AGAAGAAATT 
TTATTAGGGC CACTTGGAAT GAAAACATTA GATCCTGCAG ATTGGGCATA TAATGGATAT 
TATGATAATT CTAATGATAG TGGAGACACT AAATTAGCTC ATGGTTGGAA TTATCATCAA 
GGACCCGAAT GGATTTGGCC TATAGGTTAT TTTCTTCGAG CTCGATTGTA TTTTGCTCCA 
TTAATTGGTG GAAAAGAAGA ATTATGCCGT ACAATTGAAT CAACTGAAAC TATTATTTCT 
CGACATTTTA TAGAAGCATC TACAAATCAT TGGAGGGGTC TTCCAGAACT TACTAACAAA 
GATGGGGAAT ATTGCAAAGA TAGTTGTAGA TCGCAAGCTT GGAGTGCTGC TTCTATCCTA 
GAAGTTCTTT ATGATTTACA TAAAATCAAA CGGGAATTGC ATTCTGAGGA GAATGAAAGA 
ACGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006561264.1
CDS	227..5035
Translation	MGSILTISYFFSDATRKLIRKLRTIFQCLYKYHYLKCLYYYAVVKNMSEHMSSTESHEFEQIRVLTLNNGEHLDGILYRLKKGWRVEFRLGASLLGRTVDLFINYPLGENKKFERHTYYLLEWINETANICLSLAGSFHYYLIDPNSEGMKPIASGYLLVDPELKIGEHGNDLPLDCIQCQTVLAKCLGSFSTWEDKLLVAKNSGYNMIHFTPIQELGYSKSSYSLSDQLKLNPSFNDDNKSITYDDIEKLINKMRNEWNMLSICDIVLNHTANESPFLVSHPECTYNCLNSPHLRPAYILDAALFELTVQVAAGEWEFKGIPLIVETEEHLNSIRHALHTYFLPLIKIHELYIIDINETIAEFLNLARNQMPQDIIESSKTSEDIKIIQDSKFRRLKATINMQLALQKYNIYRTDCFDEETRLKRCAEDLRKKLQELNNIIINEIQNHLNAAVENTIAGIRYFRVQVDGPKLKEISERNPLVPRYFTDYGTPKTLTEREATMYSDAGCYLMAHNGWVMNGDPLKNFADSDSNVYIRRELIAWGDSVKLRYGDKPEDCPFLWQHISTYVEQTAKIFDGIRLDNCHSTPIPVAEYMLDVARRIRPDLYVVAELFTNSDQKDNIFVNRLGITSLIREAMSAWDSHEEGRLVYRYGGEPVGAFLQSRKRPLVPSIAHALFLDLTHDNPSTVEKRSVFDLLPSAALVSMAACASGSNRGYDELVPHHIHVVDETRQYSFWTDDECLIDGIKFVGFKTGIIAAKKALNDLHYILGQQKFSQVFVDQMDNDIVAVTRHSPTTHDSVnullLIAFTAFKHPDPNANDLRRHVKPLRVEGVMEEIILEASLCHVEAKNDISPFHYSEKYVRDESYINGLSEYIVNLKEHIQICDSEILEKVDSGDPKITQLNFVNFQPGSVIAIRVSLHANIKPALAKLHNTISIITSQKSSDIRVIASRMDLIDLNKALYRCDQEERDETSNRFDVYNIPGYGPLVYAGLQGIISLLADIRPNNDLGHPMCINLRQGNWLIDYTWQRLKEDDGTKALGEWLEEATEPFKLIPRYLVPSYFDVIVVNVYMILLEQCYNLMSSFVKDGSTFVKILSLVSIQMGGVIKSAPLPDLSPNIDSPKPKIKQYNDESEQICMTLSAGLPHFTTGYMRNWGRDTFIALRGLLILTGRHTEARFIILGYAGTLRHGLIPNLLDKGKNARYNCRDALWWWLYTIQCYIQEVPNGLKILTDKVSRLFPTDDSPPLPAGQVDQSLHDVIQEALLVHFQGLCFRERNAGKQIDEHMTDRGFNNQIGVHPETGFIFGGNDANCGTWMDKMGSSEKAGNKGKPATPRDGSAVEIVGLSKSILSFLAELYKQNLFPYGSVQRKNRDGSVVTWSYKQWADKISLNFEKYFYINEIAKEGELNLELIHRRGIFKDSHGATQAWADYQLRPNFPVAMVVAPELFNPEHAWTALKKVEEILLGPLGMKTLDPADWAYNGYYDNSNDSGDTKLAHGWNYHQGPEWIWPIGYFLRARLYFAPLIGGKEELCRTIESTETIISRHFIEASTNHWRGLPELTNKDGEYCKDSCRSQAWSAASILEVLYDLHKIKRELHSEENERTN

Target ORF information:

RefSeq Version	XM_006561201.2
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera glycogen debranching enzyme (LOC411488), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006561201.2

ORF Insert Sequence:

ATGGGTTCCA TACTTACAAT AAGCTATTTC TTTTCTGATG CAACAAGGAA ATTGATAAGG 
AAACTTAGGA CTATTTTCCA ATGCCTTTAC AAATATCATT ATTTAAAGTG CCTTTATTAT 
TATGCAGTGG TTAAAAATAT GTCAGAACAC ATGTCATCAA CTGAAAGTCA CGAATTTGAA 
CAAATAAGAG TTTTAACTCT AAATAATGGA GAACATCTTG ATGGTATATT ATATAGATTA 
AAGAAAGGAT GGAGAGTAGA ATTTCGTTTA GGCGCTTCTC TTCTTGGAAG AACTGTTGAT 
CTTTTTATAA ATTATCCTTT AGGAGAGAAT AAAAAATTTG AAAGACATAC ATATTATCTC 
TTAGAATGGA TTAATGAAAC AGCTAATATA TGTTTATCTC TAGCTGGTTC ATTTCATTAT 
TATCTTATAG ATCCTAATTC TGAAGGTATG AAGCCTATTG CTTCTGGATA TCTCCTTGTA 
GATCCAGAAC TTAAAATAGG GGAACATGGG AATGATTTAC CATTAGATTG TATTCAATGT 
CAAACTGTTC TTGCAAAATG TTTAGGATCA TTTTCTACCT GGGAAGATAA ATTACTAGTC 
GCTAAAAATT CTGGATATAA CATGATTCAT TTTACACCAA TTCAGGAATT GGGATATTCT 
AAATCTTCTT ATAGTCTTAG TGATCAATTA AAATTAAATC CTTCTTTTAA TGATGATAAT 
AAATCAATTA CATATGATGA TATTGAAAAA TTAATTAATA AAATGCGCAA TGAATGGAAT 
ATGTTAAGCA TCTGTGATAT TGTTTTGAAT CATACAGCAA ATGAAAGTCC TTTTTTGGTA 
TCTCATCCAG AATGTACATA TAATTGTTTG AATAGTCCTC ATTTACGTCC TGCATATATT 
TTGGATGCTG CATTATTTGA ATTAACAGTG CAAGTGGCTG CTGGAGAATG GGAATTTAAA 
GGCATCCCTT TGATAGTTGA AACTGAAGAA CATTTAAATT CAATTCGTCA TGCACTTCAC 
ACATATTTTT TGCCGCTTAT AAAAATACAC GAATTATATA TAATAGATAT TAATGAAACT 
ATTGCTGAGT TTTTGAATTT AGCACGAAAT CAAATGCCTC AAGACATCAT TGAATCATCA 
AAAACATCAG AAGATATTAA AATAATACAA GATTCAAAAT TTCGCAGATT AAAAGCAACT 
ATAAATATGC AACTTGCTTT ACAAAAATAT AACATATATA GAACTGATTG TTTTGATGAA 
GAGACTCGTT TGAAACGTTG TGCTGAAGAT TTAAGAAAAA AATTGCAGGA ACTTAATAAT 
ATAATCATTA ATGAAATACA AAATCATTTA AATGCTGCAG TTGAAAATAC AATTGCTGGT 
ATAAGATATT TTAGAGTGCA AGTTGATGGA CCTAAACTTA AAGAAATAAG TGAAAGAAAT 
CCACTAGTTC CTAGATATTT TACTGATTAT GGAACACCTA AAACATTAAC TGAAAGAGAA 
GCAACAATGT ATTCAGATGC AGGATGTTAT CTTATGGCTC ATAATGGATG GGTAATGAAT 
GGCGATCCTT TAAAAAATTT TGCAGATTCT GATTCTAATG TTTATATAAG AAGAGAACTT 
ATTGCTTGGG GTGATAGTGT AAAATTGAGA TATGGAGATA AACCAGAAGA TTGTCCTTTT 
CTATGGCAGC ATATTAGTAC ATATGTTGAA CAAACAGCTA AAATTTTTGA TGGTATTCGA 
TTAGACAATT GTCATTCAAC TCCTATTCCA GTTGCAGAGT ATATGCTTGA TGTTGCTCGT 
CGTATCCGTC CAGATTTGTA CGTTGTTGCA GAATTATTTA CAAATTCAGA TCAAAAAGAT 
AATATATTTG TTAATCGTCT TGGTATCACT TCTTTAATTC GAGAAGCTAT GTCTGCATGG 
GATAGCCACG AAGAAGGTCG ATTGGTATAT CGATATGGAG GTGAACCAGT CGGTGCATTT 
TTGCAATCGC GTAAACGACC TTTAGTACCA AGCATAGCAC ATGCCCTTTT TTTAGATTTA 
ACTCATGATA ATCCTAGCAC TGTGGAAAAA CGCAGCGTAT TCGATTTACT TCCAAGTGCT 
GCTCTTGTAT CAATGGCAGC ATGTGCTAGT GGTAGTAATC GTGGATATGA TGAATTAGTT 
CCTCATCATA TACATGTTGT AGATGAAACA AGACAATATT CATTTTGGAC AGATGATGAA 
TGTTTGATAG ATGGTATCAA ATTTGTAGGT TTTAAAACTG GTATAATTGC TGCAAAGAAA 
GCATTAAATG ATTTGCATTA TATTCTTGGA CAACAAAAAT TTTCTCAAGT ATTTGTTGAT 
CAAATGGATA ATGATATAGT AGCTGTAACA AGACACTCTC CAACAACTCA TGATAGTGTA 
ATNTTAATTG CTTTTACTGC TTTTAAACAT CCTGATCCTA ATGCTAATGA TTTGAGAAGA 
CATGTGAAAC CATTACGAGT GGAAGGTGTA ATGGAAGAAA TTATTTTGGA AGCATCCTTA 
TGTCATGTAG AAGCAAAAAA TGATATCTCA CCTTTCCATT ATTCTGAAAA ATATGTACGA 
GATGAAAGTT ATATAAATGG TCTCTCAGAA TATATTGTAA ATCTTAAAGA ACATATACAA 
ATTTGTGATT CAGAAATTTT GGAAAAAGTT GATTCTGGTG ATCCTAAAAT AACTCAACTT 
AATTTTGTAA ATTTTCAACC AGGTTCTGTT ATTGCTATTC GTGTATCTCT TCATGCTAAT 
ATAAAACCAG CTCTTGCAAA ACTTCATAAT ACTATTTCAA TAATCACATC CCAAAAAAGT 
TCAGACATAC GCGTTATTGC TTCACGTATG GATTTGATAG ATTTAAATAA AGCTTTATAT 
CGATGTGATC AGGAAGAACG CGATGAAACT TCAAATAGAT TTGATGTATA TAATATTCCA 
GGTTATGGAC CACTTGTTTA TGCAGGATTG CAAGGTATTA TTTCTTTATT AGCGGATATT 
CGTCCTAATA ATGATTTAGG TCATCCAATG TGTATTAATC TCAGACAAGG TAATTGGTTA 
ATAGATTATA CTTGGCAACG GTTAAAAGAA GACGATGGAA CAAAAGCACT TGGAGAATGG 
CTTGAAGAAG CTACTGAACC ATTTAAATTA ATACCTCGTT ATTTAGTTCC TAGTTATTTT 
GATGTTATAG TTGTAAACGT TTATATGATA CTTCTCGAAC AATGCTATAA TCTAATGTCA 
AGCTTTGTGA AAGATGGTTC TACTTTTGTC AAGATCTTAT CTTTGGTTTC AATACAAATG 
GGAGGAGTAA TAAAATCAGC TCCATTACCA GATTTGTCAC CAAATATAGA TTCTCCTAAA 
CCAAAGATTA AACAATATAA TGACGAAAGT GAACAAATAT GTATGACATT ATCTGCTGGT 
TTACCGCATT TCACAACAGG ATATATGAGA AATTGGGGAA GAGATACATT TATTGCTTTA 
AGAGGACTAT TGATATTAAC TGGCAGACAT ACAGAAGCAC GATTTATTAT TCTTGGTTAT 
GCTGGAACTT TACGACATGG TCTTATACCA AATTTACTTG ATAAAGGAAA GAATGCTAGA 
TATAACTGTC GAGATGCTTT ATGGTGGTGG TTGTATACAA TTCAATGTTA TATACAAGAA 
GTGCCAAATG GTTTAAAAAT TTTAACAGAT AAAGTTTCAA GACTGTTTCC AACTGATGAT 
TCTCCTCCAT TACCCGCGGG ACAAGTAGAT CAATCATTAC ATGATGTTAT TCAAGAAGCT 
CTTTTAGTAC ATTTTCAAGG ACTTTGTTTT AGAGAAAGAA ATGCTGGAAA ACAGATTGAT 
GAACATATGA CAGATCGTGG TTTTAATAAT CAAATTGGAG TACATCCAGA AACAGGATTT 
ATATTTGGTG GAAATGATGC AAATTGTGGT ACGTGGATGG ATAAAATGGG ATCTTCTGAA 
AAAGCTGGTA ACAAAGGAAA ACCAGCTACT CCAAGGGATG GTTCAGCTGT AGAAATAGTG 
GGTCTCAGTA AAAGTATTCT TAGTTTTTTA GCTGAATTAT ATAAACAAAA TCTTTTTCCT 
TATGGTAGTG TGCAAAGAAA AAATCGTGAT GGTTCTGTTG TAACATGGAG TTATAAACAA 
TGGGCAGATA AAATTTCATT AAATTTTGAA AAATATTTTT ATATTAATGA AATAGCAAAA 
GAAGGAGAAT TAAATTTAGA ACTTATTCAT CGGCGTGGAA TTTTTAAAGA TAGTCATGGA 
GCAACACAAG CATGGGCTGA TTATCAACTA CGACCTAATT TTCCAGTTGC AATGGTTGTT 
GCTCCAGAGT TATTTAATCC AGAACATGCA TGGACAGCAT TAAAAAAAGT AGAAGAAATT 
TTATTAGGGC CACTTGGAAT GAAAACATTA GATCCTGCAG ATTGGGCATA TAATGGATAT 
TATGATAATT CTAATGATAG TGGAGACACT AAATTAGCTC ATGGTTGGAA TTATCATCAA 
GGACCCGAAT GGATTTGGCC TATAGGTTAT TTTCTTCGAG CTCGATTGTA TTTTGCTCCA 
TTAATTGGTG GAAAAGAAGA ATTATGCCGT ACAATTGAAT CAACTGAAAC TATTATTTCT 
CGACATTTTA TAGAAGCATC TACAAATCAT TGGAGGGGTC TTCCAGAACT TACTAACAAA 
GATGGGGAAT ATTGCAAAGA TAGTTGTAGA TCGCAAGCTT GGAGTGCTGC TTCTATCCTA 
GAAGTTCTTT ATGATTTACA TAAAATCAAA CGGGAATTGC ATTCTGAGGA GAATGAAAGA 
ACGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC411488 ( XM_016915600.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_016771089.1 Apis mellifera glycogen debranching enzyme (LOC411488), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHd13284
Clone ID Related Accession (Same CDS sequence)	XM_006561201.2 , XM_016915600.1
Accession Version	XM_016915600.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1463932800000
Organism	Apis mellifera(honey bee)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007081.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis mellifera Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA TACTTACAAT AAGCTATTTC TTTTCTGATG CAACAAGGAA ATTGATAAGG 
AAACTTAGGA CTATTTTCCA ATGCCTTTAC AAATATCATT ATTTAAAGTG CCTTTATTAT 
TATGCAGTGG TTAAAAATAT GTCAGAACAC ATGTCATCAA CTGAAAGTCA CGAATTTGAA 
CAAATAAGAG TTTTAACTCT AAATAATGGA GAACATCTTG ATGGTATATT ATATAGATTA 
AAGAAAGGAT GGAGAGTAGA ATTTCGTTTA GGCGCTTCTC TTCTTGGAAG AACTGTTGAT 
CTTTTTATAA ATTATCCTTT AGGAGAGAAT AAAAAATTTG AAAGACATAC ATATTATCTC 
TTAGAATGGA TTAATGAAAC AGCTAATATA TGTTTATCTC TAGCTGGTTC ATTTCATTAT 
TATCTTATAG ATCCTAATTC TGAAGGTATG AAGCCTATTG CTTCTGGATA TCTCCTTGTA 
GATCCAGAAC TTAAAATAGG GGAACATGGG AATGATTTAC CATTAGATTG TATTCAATGT 
CAAACTGTTC TTGCAAAATG TTTAGGATCA TTTTCTACCT GGGAAGATAA ATTACTAGTC 
GCTAAAAATT CTGGATATAA CATGATTCAT TTTACACCAA TTCAGGAATT GGGATATTCT 
AAATCTTCTT ATAGTCTTAG TGATCAATTA AAATTAAATC CTTCTTTTAA TGATGATAAT 
AAATCAATTA CATATGATGA TATTGAAAAA TTAATTAATA AAATGCGCAA TGAATGGAAT 
ATGTTAAGCA TCTGTGATAT TGTTTTGAAT CATACAGCAA ATGAAAGTCC TTTTTTGGTA 
TCTCATCCAG AATGTACATA TAATTGTTTG AATAGTCCTC ATTTACGTCC TGCATATATT 
TTGGATGCTG CATTATTTGA ATTAACAGTG CAAGTGGCTG CTGGAGAATG GGAATTTAAA 
GGCATCCCTT TGATAGTTGA AACTGAAGAA CATTTAAATT CAATTCGTCA TGCACTTCAC 
ACATATTTTT TGCCGCTTAT AAAAATACAC GAATTATATA TAATAGATAT TAATGAAACT 
ATTGCTGAGT TTTTGAATTT AGCACGAAAT CAAATGCCTC AAGACATCAT TGAATCATCA 
AAAACATCAG AAGATATTAA AATAATACAA GATTCAAAAT TTCGCAGATT AAAAGCAACT 
ATAAATATGC AACTTGCTTT ACAAAAATAT AACATATATA GAACTGATTG TTTTGATGAA 
GAGACTCGTT TGAAACGTTG TGCTGAAGAT TTAAGAAAAA AATTGCAGGA ACTTAATAAT 
ATAATCATTA ATGAAATACA AAATCATTTA AATGCTGCAG TTGAAAATAC AATTGCTGGT 
ATAAGATATT TTAGAGTGCA AGTTGATGGA CCTAAACTTA AAGAAATAAG TGAAAGAAAT 
CCACTAGTTC CTAGATATTT TACTGATTAT GGAACACCTA AAACATTAAC TGAAAGAGAA 
GCAACAATGT ATTCAGATGC AGGATGTTAT CTTATGGCTC ATAATGGATG GGTAATGAAT 
GGCGATCCTT TAAAAAATTT TGCAGATTCT GATTCTAATG TTTATATAAG AAGAGAACTT 
ATTGCTTGGG GTGATAGTGT AAAATTGAGA TATGGAGATA AACCAGAAGA TTGTCCTTTT 
CTATGGCAGC ATATTAGTAC ATATGTTGAA CAAACAGCTA AAATTTTTGA TGGTATTCGA 
TTAGACAATT GTCATTCAAC TCCTATTCCA GTTGCAGAGT ATATGCTTGA TGTTGCTCGT 
CGTATCCGTC CAGATTTGTA CGTTGTTGCA GAATTATTTA CAAATTCAGA TCAAAAAGAT 
AATATATTTG TTAATCGTCT TGGTATCACT TCTTTAATTC GAGAAGCTAT GTCTGCATGG 
GATAGCCACG AAGAAGGTCG ATTGGTATAT CGATATGGAG GTGAACCAGT CGGTGCATTT 
TTGCAATCGC GTAAACGACC TTTAGTACCA AGCATAGCAC ATGCCCTTTT TTTAGATTTA 
ACTCATGATA ATCCTAGCAC TGTGGAAAAA CGCAGCGTAT TCGATTTACT TCCAAGTGCT 
GCTCTTGTAT CAATGGCAGC ATGTGCTAGT GGTAGTAATC GTGGATATGA TGAATTAGTT 
CCTCATCATA TACATGTTGT AGATGAAACA AGACAATATT CATTTTGGAC AGATGATGAA 
TGTTTGATAG ATGGTATCAA ATTTGTAGGT TTTAAAACTG GTATAATTGC TGCAAAGAAA 
GCATTAAATG ATTTGCATTA TATTCTTGGA CAACAAAAAT TTTCTCAAGT ATTTGTTGAT 
CAAATGGATA ATGATATAGT AGCTGTAACA AGACACTCTC CAACAACTCA TGATAGTGTA 
ATNTTAATTG CTTTTACTGC TTTTAAACAT CCTGATCCTA ATGCTAATGA TTTGAGAAGA 
CATGTGAAAC CATTACGAGT GGAAGGTGTA ATGGAAGAAA TTATTTTGGA AGCATCCTTA 
TGTCATGTAG AAGCAAAAAA TGATATCTCA CCTTTCCATT ATTCTGAAAA ATATGTACGA 
GATGAAAGTT ATATAAATGG TCTCTCAGAA TATATTGTAA ATCTTAAAGA ACATATACAA 
ATTTGTGATT CAGAAATTTT GGAAAAAGTT GATTCTGGTG ATCCTAAAAT AACTCAACTT 
AATTTTGTAA ATTTTCAACC AGGTTCTGTT ATTGCTATTC GTGTATCTCT TCATGCTAAT 
ATAAAACCAG CTCTTGCAAA ACTTCATAAT ACTATTTCAA TAATCACATC CCAAAAAAGT 
TCAGACATAC GCGTTATTGC TTCACGTATG GATTTGATAG ATTTAAATAA AGCTTTATAT 
CGATGTGATC AGGAAGAACG CGATGAAACT TCAAATAGAT TTGATGTATA TAATATTCCA 
GGTTATGGAC CACTTGTTTA TGCAGGATTG CAAGGTATTA TTTCTTTATT AGCGGATATT 
CGTCCTAATA ATGATTTAGG TCATCCAATG TGTATTAATC TCAGACAAGG TAATTGGTTA 
ATAGATTATA CTTGGCAACG GTTAAAAGAA GACGATGGAA CAAAAGCACT TGGAGAATGG 
CTTGAAGAAG CTACTGAACC ATTTAAATTA ATACCTCGTT ATTTAGTTCC TAGTTATTTT 
GATGTTATAG TTGTAAACGT TTATATGATA CTTCTCGAAC AATGCTATAA TCTAATGTCA 
AGCTTTGTGA AAGATGGTTC TACTTTTGTC AAGATCTTAT CTTTGGTTTC AATACAAATG 
GGAGGAGTAA TAAAATCAGC TCCATTACCA GATTTGTCAC CAAATATAGA TTCTCCTAAA 
CCAAAGATTA AACAATATAA TGACGAAAGT GAACAAATAT GTATGACATT ATCTGCTGGT 
TTACCGCATT TCACAACAGG ATATATGAGA AATTGGGGAA GAGATACATT TATTGCTTTA 
AGAGGACTAT TGATATTAAC TGGCAGACAT ACAGAAGCAC GATTTATTAT TCTTGGTTAT 
GCTGGAACTT TACGACATGG TCTTATACCA AATTTACTTG ATAAAGGAAA GAATGCTAGA 
TATAACTGTC GAGATGCTTT ATGGTGGTGG TTGTATACAA TTCAATGTTA TATACAAGAA 
GTGCCAAATG GTTTAAAAAT TTTAACAGAT AAAGTTTCAA GACTGTTTCC AACTGATGAT 
TCTCCTCCAT TACCCGCGGG ACAAGTAGAT CAATCATTAC ATGATGTTAT TCAAGAAGCT 
CTTTTAGTAC ATTTTCAAGG ACTTTGTTTT AGAGAAAGAA ATGCTGGAAA ACAGATTGAT 
GAACATATGA CAGATCGTGG TTTTAATAAT CAAATTGGAG TACATCCAGA AACAGGATTT 
ATATTTGGTG GAAATGATGC AAATTGTGGT ACGTGGATGG ATAAAATGGG ATCTTCTGAA 
AAAGCTGGTA ACAAAGGAAA ACCAGCTACT CCAAGGGATG GTTCAGCTGT AGAAATAGTG 
GGTCTCAGTA AAAGTATTCT TAGTTTTTTA GCTGAATTAT ATAAACAAAA TCTTTTTCCT 
TATGGTAGTG TGCAAAGAAA AAATCGTGAT GGTTCTGTTG TAACATGGAG TTATAAACAA 
TGGGCAGATA AAATTTCATT AAATTTTGAA AAATATTTTT ATATTAATGA AATAGCAAAA 
GAAGGAGAAT TAAATTTAGA ACTTATTCAT CGGCGTGGAA TTTTTAAAGA TAGTCATGGA 
GCAACACAAG CATGGGCTGA TTATCAACTA CGACCTAATT TTCCAGTTGC AATGGTTGTT 
GCTCCAGAGT TATTTAATCC AGAACATGCA TGGACAGCAT TAAAAAAAGT AGAAGAAATT 
TTATTAGGGC CACTTGGAAT GAAAACATTA GATCCTGCAG ATTGGGCATA TAATGGATAT 
TATGATAATT CTAATGATAG TGGAGACACT AAATTAGCTC ATGGTTGGAA TTATCATCAA 
GGACCCGAAT GGATTTGGCC TATAGGTTAT TTTCTTCGAG CTCGATTGTA TTTTGCTCCA 
TTAATTGGTG GAAAAGAAGA ATTATGCCGT ACAATTGAAT CAACTGAAAC TATTATTTCT 
CGACATTTTA TAGAAGCATC TACAAATCAT TGGAGGGGTC TTCCAGAACT TACTAACAAA 
GATGGGGAAT ATTGCAAAGA TAGTTGTAGA TCGCAAGCTT GGAGTGCTGC TTCTATCCTA 
GAAGTTCTTT ATGATTTACA TAAAATCAAA CGGGAATTGC ATTCTGAGGA GAATGAAAGA 
ACGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016771089.1
CDS	140..4948
Translation	MGSILTISYFFSDATRKLIRKLRTIFQCLYKYHYLKCLYYYAVVKNMSEHMSSTESHEFEQIRVLTLNNGEHLDGILYRLKKGWRVEFRLGASLLGRTVDLFINYPLGENKKFERHTYYLLEWINETANICLSLAGSFHYYLIDPNSEGMKPIASGYLLVDPELKIGEHGNDLPLDCIQCQTVLAKCLGSFSTWEDKLLVAKNSGYNMIHFTPIQELGYSKSSYSLSDQLKLNPSFNDDNKSITYDDIEKLINKMRNEWNMLSICDIVLNHTANESPFLVSHPECTYNCLNSPHLRPAYILDAALFELTVQVAAGEWEFKGIPLIVETEEHLNSIRHALHTYFLPLIKIHELYIIDINETIAEFLNLARNQMPQDIIESSKTSEDIKIIQDSKFRRLKATINMQLALQKYNIYRTDCFDEETRLKRCAEDLRKKLQELNNIIINEIQNHLNAAVENTIAGIRYFRVQVDGPKLKEISERNPLVPRYFTDYGTPKTLTEREATMYSDAGCYLMAHNGWVMNGDPLKNFADSDSNVYIRRELIAWGDSVKLRYGDKPEDCPFLWQHISTYVEQTAKIFDGIRLDNCHSTPIPVAEYMLDVARRIRPDLYVVAELFTNSDQKDNIFVNRLGITSLIREAMSAWDSHEEGRLVYRYGGEPVGAFLQSRKRPLVPSIAHALFLDLTHDNPSTVEKRSVFDLLPSAALVSMAACASGSNRGYDELVPHHIHVVDETRQYSFWTDDECLIDGIKFVGFKTGIIAAKKALNDLHYILGQQKFSQVFVDQMDNDIVAVTRHSPTTHDSVnullLIAFTAFKHPDPNANDLRRHVKPLRVEGVMEEIILEASLCHVEAKNDISPFHYSEKYVRDESYINGLSEYIVNLKEHIQICDSEILEKVDSGDPKITQLNFVNFQPGSVIAIRVSLHANIKPALAKLHNTISIITSQKSSDIRVIASRMDLIDLNKALYRCDQEERDETSNRFDVYNIPGYGPLVYAGLQGIISLLADIRPNNDLGHPMCINLRQGNWLIDYTWQRLKEDDGTKALGEWLEEATEPFKLIPRYLVPSYFDVIVVNVYMILLEQCYNLMSSFVKDGSTFVKILSLVSIQMGGVIKSAPLPDLSPNIDSPKPKIKQYNDESEQICMTLSAGLPHFTTGYMRNWGRDTFIALRGLLILTGRHTEARFIILGYAGTLRHGLIPNLLDKGKNARYNCRDALWWWLYTIQCYIQEVPNGLKILTDKVSRLFPTDDSPPLPAGQVDQSLHDVIQEALLVHFQGLCFRERNAGKQIDEHMTDRGFNNQIGVHPETGFIFGGNDANCGTWMDKMGSSEKAGNKGKPATPRDGSAVEIVGLSKSILSFLAELYKQNLFPYGSVQRKNRDGSVVTWSYKQWADKISLNFEKYFYINEIAKEGELNLELIHRRGIFKDSHGATQAWADYQLRPNFPVAMVVAPELFNPEHAWTALKKVEEILLGPLGMKTLDPADWAYNGYYDNSNDSGDTKLAHGWNYHQGPEWIWPIGYFLRARLYFAPLIGGKEELCRTIESTETIISRHFIEASTNHWRGLPELTNKDGEYCKDSCRSQAWSAASILEVLYDLHKIKRELHSEENERTN

Target ORF information:

RefSeq Version	XM_016915600.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera glycogen debranching enzyme (LOC411488), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016915600.1

ORF Insert Sequence:

ATGGGTTCCA TACTTACAAT AAGCTATTTC TTTTCTGATG CAACAAGGAA ATTGATAAGG 
AAACTTAGGA CTATTTTCCA ATGCCTTTAC AAATATCATT ATTTAAAGTG CCTTTATTAT 
TATGCAGTGG TTAAAAATAT GTCAGAACAC ATGTCATCAA CTGAAAGTCA CGAATTTGAA 
CAAATAAGAG TTTTAACTCT AAATAATGGA GAACATCTTG ATGGTATATT ATATAGATTA 
AAGAAAGGAT GGAGAGTAGA ATTTCGTTTA GGCGCTTCTC TTCTTGGAAG AACTGTTGAT 
CTTTTTATAA ATTATCCTTT AGGAGAGAAT AAAAAATTTG AAAGACATAC ATATTATCTC 
TTAGAATGGA TTAATGAAAC AGCTAATATA TGTTTATCTC TAGCTGGTTC ATTTCATTAT 
TATCTTATAG ATCCTAATTC TGAAGGTATG AAGCCTATTG CTTCTGGATA TCTCCTTGTA 
GATCCAGAAC TTAAAATAGG GGAACATGGG AATGATTTAC CATTAGATTG TATTCAATGT 
CAAACTGTTC TTGCAAAATG TTTAGGATCA TTTTCTACCT GGGAAGATAA ATTACTAGTC 
GCTAAAAATT CTGGATATAA CATGATTCAT TTTACACCAA TTCAGGAATT GGGATATTCT 
AAATCTTCTT ATAGTCTTAG TGATCAATTA AAATTAAATC CTTCTTTTAA TGATGATAAT 
AAATCAATTA CATATGATGA TATTGAAAAA TTAATTAATA AAATGCGCAA TGAATGGAAT 
ATGTTAAGCA TCTGTGATAT TGTTTTGAAT CATACAGCAA ATGAAAGTCC TTTTTTGGTA 
TCTCATCCAG AATGTACATA TAATTGTTTG AATAGTCCTC ATTTACGTCC TGCATATATT 
TTGGATGCTG CATTATTTGA ATTAACAGTG CAAGTGGCTG CTGGAGAATG GGAATTTAAA 
GGCATCCCTT TGATAGTTGA AACTGAAGAA CATTTAAATT CAATTCGTCA TGCACTTCAC 
ACATATTTTT TGCCGCTTAT AAAAATACAC GAATTATATA TAATAGATAT TAATGAAACT 
ATTGCTGAGT TTTTGAATTT AGCACGAAAT CAAATGCCTC AAGACATCAT TGAATCATCA 
AAAACATCAG AAGATATTAA AATAATACAA GATTCAAAAT TTCGCAGATT AAAAGCAACT 
ATAAATATGC AACTTGCTTT ACAAAAATAT AACATATATA GAACTGATTG TTTTGATGAA 
GAGACTCGTT TGAAACGTTG TGCTGAAGAT TTAAGAAAAA AATTGCAGGA ACTTAATAAT 
ATAATCATTA ATGAAATACA AAATCATTTA AATGCTGCAG TTGAAAATAC AATTGCTGGT 
ATAAGATATT TTAGAGTGCA AGTTGATGGA CCTAAACTTA AAGAAATAAG TGAAAGAAAT 
CCACTAGTTC CTAGATATTT TACTGATTAT GGAACACCTA AAACATTAAC TGAAAGAGAA 
GCAACAATGT ATTCAGATGC AGGATGTTAT CTTATGGCTC ATAATGGATG GGTAATGAAT 
GGCGATCCTT TAAAAAATTT TGCAGATTCT GATTCTAATG TTTATATAAG AAGAGAACTT 
ATTGCTTGGG GTGATAGTGT AAAATTGAGA TATGGAGATA AACCAGAAGA TTGTCCTTTT 
CTATGGCAGC ATATTAGTAC ATATGTTGAA CAAACAGCTA AAATTTTTGA TGGTATTCGA 
TTAGACAATT GTCATTCAAC TCCTATTCCA GTTGCAGAGT ATATGCTTGA TGTTGCTCGT 
CGTATCCGTC CAGATTTGTA CGTTGTTGCA GAATTATTTA CAAATTCAGA TCAAAAAGAT 
AATATATTTG TTAATCGTCT TGGTATCACT TCTTTAATTC GAGAAGCTAT GTCTGCATGG 
GATAGCCACG AAGAAGGTCG ATTGGTATAT CGATATGGAG GTGAACCAGT CGGTGCATTT 
TTGCAATCGC GTAAACGACC TTTAGTACCA AGCATAGCAC ATGCCCTTTT TTTAGATTTA 
ACTCATGATA ATCCTAGCAC TGTGGAAAAA CGCAGCGTAT TCGATTTACT TCCAAGTGCT 
GCTCTTGTAT CAATGGCAGC ATGTGCTAGT GGTAGTAATC GTGGATATGA TGAATTAGTT 
CCTCATCATA TACATGTTGT AGATGAAACA AGACAATATT CATTTTGGAC AGATGATGAA 
TGTTTGATAG ATGGTATCAA ATTTGTAGGT TTTAAAACTG GTATAATTGC TGCAAAGAAA 
GCATTAAATG ATTTGCATTA TATTCTTGGA CAACAAAAAT TTTCTCAAGT ATTTGTTGAT 
CAAATGGATA ATGATATAGT AGCTGTAACA AGACACTCTC CAACAACTCA TGATAGTGTA 
ATNTTAATTG CTTTTACTGC TTTTAAACAT CCTGATCCTA ATGCTAATGA TTTGAGAAGA 
CATGTGAAAC CATTACGAGT GGAAGGTGTA ATGGAAGAAA TTATTTTGGA AGCATCCTTA 
TGTCATGTAG AAGCAAAAAA TGATATCTCA CCTTTCCATT ATTCTGAAAA ATATGTACGA 
GATGAAAGTT ATATAAATGG TCTCTCAGAA TATATTGTAA ATCTTAAAGA ACATATACAA 
ATTTGTGATT CAGAAATTTT GGAAAAAGTT GATTCTGGTG ATCCTAAAAT AACTCAACTT 
AATTTTGTAA ATTTTCAACC AGGTTCTGTT ATTGCTATTC GTGTATCTCT TCATGCTAAT 
ATAAAACCAG CTCTTGCAAA ACTTCATAAT ACTATTTCAA TAATCACATC CCAAAAAAGT 
TCAGACATAC GCGTTATTGC TTCACGTATG GATTTGATAG ATTTAAATAA AGCTTTATAT 
CGATGTGATC AGGAAGAACG CGATGAAACT TCAAATAGAT TTGATGTATA TAATATTCCA 
GGTTATGGAC CACTTGTTTA TGCAGGATTG CAAGGTATTA TTTCTTTATT AGCGGATATT 
CGTCCTAATA ATGATTTAGG TCATCCAATG TGTATTAATC TCAGACAAGG TAATTGGTTA 
ATAGATTATA CTTGGCAACG GTTAAAAGAA GACGATGGAA CAAAAGCACT TGGAGAATGG 
CTTGAAGAAG CTACTGAACC ATTTAAATTA ATACCTCGTT ATTTAGTTCC TAGTTATTTT 
GATGTTATAG TTGTAAACGT TTATATGATA CTTCTCGAAC AATGCTATAA TCTAATGTCA 
AGCTTTGTGA AAGATGGTTC TACTTTTGTC AAGATCTTAT CTTTGGTTTC AATACAAATG 
GGAGGAGTAA TAAAATCAGC TCCATTACCA GATTTGTCAC CAAATATAGA TTCTCCTAAA 
CCAAAGATTA AACAATATAA TGACGAAAGT GAACAAATAT GTATGACATT ATCTGCTGGT 
TTACCGCATT TCACAACAGG ATATATGAGA AATTGGGGAA GAGATACATT TATTGCTTTA 
AGAGGACTAT TGATATTAAC TGGCAGACAT ACAGAAGCAC GATTTATTAT TCTTGGTTAT 
GCTGGAACTT TACGACATGG TCTTATACCA AATTTACTTG ATAAAGGAAA GAATGCTAGA 
TATAACTGTC GAGATGCTTT ATGGTGGTGG TTGTATACAA TTCAATGTTA TATACAAGAA 
GTGCCAAATG GTTTAAAAAT TTTAACAGAT AAAGTTTCAA GACTGTTTCC AACTGATGAT 
TCTCCTCCAT TACCCGCGGG ACAAGTAGAT CAATCATTAC ATGATGTTAT TCAAGAAGCT 
CTTTTAGTAC ATTTTCAAGG ACTTTGTTTT AGAGAAAGAA ATGCTGGAAA ACAGATTGAT 
GAACATATGA CAGATCGTGG TTTTAATAAT CAAATTGGAG TACATCCAGA AACAGGATTT 
ATATTTGGTG GAAATGATGC AAATTGTGGT ACGTGGATGG ATAAAATGGG ATCTTCTGAA 
AAAGCTGGTA ACAAAGGAAA ACCAGCTACT CCAAGGGATG GTTCAGCTGT AGAAATAGTG 
GGTCTCAGTA AAAGTATTCT TAGTTTTTTA GCTGAATTAT ATAAACAAAA TCTTTTTCCT 
TATGGTAGTG TGCAAAGAAA AAATCGTGAT GGTTCTGTTG TAACATGGAG TTATAAACAA 
TGGGCAGATA AAATTTCATT AAATTTTGAA AAATATTTTT ATATTAATGA AATAGCAAAA 
GAAGGAGAAT TAAATTTAGA ACTTATTCAT CGGCGTGGAA TTTTTAAAGA TAGTCATGGA 
GCAACACAAG CATGGGCTGA TTATCAACTA CGACCTAATT TTCCAGTTGC AATGGTTGTT 
GCTCCAGAGT TATTTAATCC AGAACATGCA TGGACAGCAT TAAAAAAAGT AGAAGAAATT 
TTATTAGGGC CACTTGGAAT GAAAACATTA GATCCTGCAG ATTGGGCATA TAATGGATAT 
TATGATAATT CTAATGATAG TGGAGACACT AAATTAGCTC ATGGTTGGAA TTATCATCAA 
GGACCCGAAT GGATTTGGCC TATAGGTTAT TTTCTTCGAG CTCGATTGTA TTTTGCTCCA 
TTAATTGGTG GAAAAGAAGA ATTATGCCGT ACAATTGAAT CAACTGAAAC TATTATTTCT 
CGACATTTTA TAGAAGCATC TACAAATCAT TGGAGGGGTC TTCCAGAACT TACTAACAAA 
GATGGGGAAT ATTGCAAAGA TAGTTGTAGA TCGCAAGCTT GGAGTGCTGC TTCTATCCTA 
GAAGTTCTTT ATGATTTACA TAAAATCAAA CGGGAATTGC ATTCTGAGGA GAATGAAAGA 
ACGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC411488 ( XM_006561204.2 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_006561267.1 Apis mellifera glycogen debranching enzyme (LOC411488), transcript variant X5, mRNA.

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CloneID	OHd13286
Clone ID Related Accession (Same CDS sequence)	XM_006561204.2 , XM_016915601.1 , XM_006561203.2
Accession Version	XM_006561204.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1463932800000
Organism	Apis mellifera(honey bee)
Product	glycogen debranching enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007081.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 24, 2016 this sequence version replaced XM_006561204.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis mellifera Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAC ACATGTCATC AACTGAAAGT CACGAATTTG AACAAATAAG AGTTTTAACT 
CTAAATAATG GAGAACATCT TGATGGTATA TTATATAGAT TAAAGAAAGG ATGGAGAGTA 
GAATTTCGTT TAGGCGCTTC TCTTCTTGGA AGAACTGTTG ATCTTTTTAT AAATTATCCT 
TTAGGAGAGA ATAAAAAATT TGAAAGACAT ACATATTATC TCTTAGAATG GATTAATGAA 
ACAGCTAATA TATGTTTATC TCTAGCTGGT TCATTTCATT ATTATCTTAT AGATCCTAAT 
TCTGAAGGTA TGAAGCCTAT TGCTTCTGGA TATCTCCTTG TAGATCCAGA ACTTAAAATA 
GGGGAACATG GGAATGATTT ACCATTAGAT TGTATTCAAT GTCAAACTGT TCTTGCAAAA 
TGTTTAGGAT CATTTTCTAC CTGGGAAGAT AAATTACTAG TCGCTAAAAA TTCTGGATAT 
AACATGATTC ATTTTACACC AATTCAGGAA TTGGGATATT CTAAATCTTC TTATAGTCTT 
AGTGATCAAT TAAAATTAAA TCCTTCTTTT AATGATGATA ATAAATCAAT TACATATGAT 
GATATTGAAA AATTAATTAA TAAAATGCGC AATGAATGGA ATATGTTAAG CATCTGTGAT 
ATTGTTTTGA ATCATACAGC AAATGAAAGT CCTTTTTTGG TATCTCATCC AGAATGTACA 
TATAATTGTT TGAATAGTCC TCATTTACGT CCTGCATATA TTTTGGATGC TGCATTATTT 
GAATTAACAG TGCAAGTGGC TGCTGGAGAA TGGGAATTTA AAGGCATCCC TTTGATAGTT 
GAAACTGAAG AACATTTAAA TTCAATTCGT CATGCACTTC ACACATATTT TTTGCCGCTT 
ATAAAAATAC ACGAATTATA TATAATAGAT ATTAATGAAA CTATTGCTGA GTTTTTGAAT 
TTAGCACGAA ATCAAATGCC TCAAGACATC ATTGAATCAT CAAAAACATC AGAAGATATT 
AAAATAATAC AAGATTCAAA ATTTCGCAGA TTAAAAGCAA CTATAAATAT GCAACTTGCT 
TTACAAAAAT ATAACATATA TAGAACTGAT TGTTTTGATG AAGAGACTCG TTTGAAACGT 
TGTGCTGAAG ATTTAAGAAA AAAATTGCAG GAACTTAATA ATATAATCAT TAATGAAATA 
CAAAATCATT TAAATGCTGC AGTTGAAAAT ACAATTGCTG GTATAAGATA TTTTAGAGTG 
CAAGTTGATG GACCTAAACT TAAAGAAATA AGTGAAAGAA ATCCACTAGT TCCTAGATAT 
TTTACTGATT ATGGAACACC TAAAACATTA ACTGAAAGAG AAGCAACAAT GTATTCAGAT 
GCAGGATGTT ATCTTATGGC TCATAATGGA TGGGTAATGA ATGGCGATCC TTTAAAAAAT 
TTTGCAGATT CTGATTCTAA TGTTTATATA AGAAGAGAAC TTATTGCTTG GGGTGATAGT 
GTAAAATTGA GATATGGAGA TAAACCAGAA GATTGTCCTT TTCTATGGCA GCATATTAGT 
ACATATGTTG AACAAACAGC TAAAATTTTT GATGGTATTC GATTAGACAA TTGTCATTCA 
ACTCCTATTC CAGTTGCAGA GTATATGCTT GATGTTGCTC GTCGTATCCG TCCAGATTTG 
TACGTTGTTG CAGAATTATT TACAAATTCA GATCAAAAAG ATAATATATT TGTTAATCGT 
CTTGGTATCA CTTCTTTAAT TCGAGAAGCT ATGTCTGCAT GGGATAGCCA CGAAGAAGGT 
CGATTGGTAT ATCGATATGG AGGTGAACCA GTCGGTGCAT TTTTGCAATC GCGTAAACGA 
CCTTTAGTAC CAAGCATAGC ACATGCCCTT TTTTTAGATT TAACTCATGA TAATCCTAGC 
ACTGTGGAAA AACGCAGCGT ATTCGATTTA CTTCCAAGTG CTGCTCTTGT ATCAATGGCA 
GCATGTGCTA GTGGTAGTAA TCGTGGATAT GATGAATTAG TTCCTCATCA TATACATGTT 
GTAGATGAAA CAAGACAATA TTCATTTTGG ACAGATGATG AATGTTTGAT AGATGGTATC 
AAATTTGTAG GTTTTAAAAC TGGTATAATT GCTGCAAAGA AAGCATTAAA TGATTTGCAT 
TATATTCTTG GACAACAAAA ATTTTCTCAA GTATTTGTTG ATCAAATGGA TAATGATATA 
GTAGCTGTAA CAAGACACTC TCCAACAACT CATGATAGTG TAATNTTAAT TGCTTTTACT 
GCTTTTAAAC ATCCTGATCC TAATGCTAAT GATTTGAGAA GACATGTGAA ACCATTACGA 
GTGGAAGGTG TAATGGAAGA AATTATTTTG GAAGCATCCT TATGTCATGT AGAAGCAAAA 
AATGATATCT CACCTTTCCA TTATTCTGAA AAATATGTAC GAGATGAAAG TTATATAAAT 
GGTCTCTCAG AATATATTGT AAATCTTAAA GAACATATAC AAATTTGTGA TTCAGAAATT 
TTGGAAAAAG TTGATTCTGG TGATCCTAAA ATAACTCAAC TTAATTTTGT AAATTTTCAA 
CCAGGTTCTG TTATTGCTAT TCGTGTATCT CTTCATGCTA ATATAAAACC AGCTCTTGCA 
AAACTTCATA ATACTATTTC AATAATCACA TCCCAAAAAA GTTCAGACAT ACGCGTTATT 
GCTTCACGTA TGGATTTGAT AGATTTAAAT AAAGCTTTAT ATCGATGTGA TCAGGAAGAA 
CGCGATGAAA CTTCAAATAG ATTTGATGTA TATAATATTC CAGGTTATGG ACCACTTGTT 
TATGCAGGAT TGCAAGGTAT TATTTCTTTA TTAGCGGATA TTCGTCCTAA TAATGATTTA 
GGTCATCCAA TGTGTATTAA TCTCAGACAA GGTAATTGGT TAATAGATTA TACTTGGCAA 
CGGTTAAAAG AAGACGATGG AACAAAAGCA CTTGGAGAAT GGCTTGAAGA AGCTACTGAA 
CCATTTAAAT TAATACCTCG TTATTTAGTT CCTAGTTATT TTGATGTTAT AGTTGTAAAC 
GTTTATATGA TACTTCTCGA ACAATGCTAT AATCTAATGT CAAGCTTTGT GAAAGATGGT 
TCTACTTTTG TCAAGATCTT ATCTTTGGTT TCAATACAAA TGGGAGGAGT AATAAAATCA 
GCTCCATTAC CAGATTTGTC ACCAAATATA GATTCTCCTA AACCAAAGAT TAAACAATAT 
AATGACGAAA GTGAACAAAT ATGTATGACA TTATCTGCTG GTTTACCGCA TTTCACAACA 
GGATATATGA GAAATTGGGG AAGAGATACA TTTATTGCTT TAAGAGGACT ATTGATATTA 
ACTGGCAGAC ATACAGAAGC ACGATTTATT ATTCTTGGTT ATGCTGGAAC TTTACGACAT 
GGTCTTATAC CAAATTTACT TGATAAAGGA AAGAATGCTA GATATAACTG TCGAGATGCT 
TTATGGTGGT GGTTGTATAC AATTCAATGT TATATACAAG AAGTGCCAAA TGGTTTAAAA 
ATTTTAACAG ATAAAGTTTC AAGACTGTTT CCAACTGATG ATTCTCCTCC ATTACCCGCG 
GGACAAGTAG ATCAATCATT ACATGATGTT ATTCAAGAAG CTCTTTTAGT ACATTTTCAA 
GGACTTTGTT TTAGAGAAAG AAATGCTGGA AAACAGATTG ATGAACATAT GACAGATCGT 
GGTTTTAATA ATCAAATTGG AGTACATCCA GAAACAGGAT TTATATTTGG TGGAAATGAT 
GCAAATTGTG GTACGTGGAT GGATAAAATG GGATCTTCTG AAAAAGCTGG TAACAAAGGA 
AAACCAGCTA CTCCAAGGGA TGGTTCAGCT GTAGAAATAG TGGGTCTCAG TAAAAGTATT 
CTTAGTTTTT TAGCTGAATT ATATAAACAA AATCTTTTTC CTTATGGTAG TGTGCAAAGA 
AAAAATCGTG ATGGTTCTGT TGTAACATGG AGTTATAAAC AATGGGCAGA TAAAATTTCA 
TTAAATTTTG AAAAATATTT TTATATTAAT GAAATAGCAA AAGAAGGAGA ATTAAATTTA 
GAACTTATTC ATCGGCGTGG AATTTTTAAA GATAGTCATG GAGCAACACA AGCATGGGCT 
GATTATCAAC TACGACCTAA TTTTCCAGTT GCAATGGTTG TTGCTCCAGA GTTATTTAAT 
CCAGAACATG CATGGACAGC ATTAAAAAAA GTAGAAGAAA TTTTATTAGG GCCACTTGGA 
ATGAAAACAT TAGATCCTGC AGATTGGGCA TATAATGGAT ATTATGATAA TTCTAATGAT 
AGTGGAGACA CTAAATTAGC TCATGGTTGG AATTATCATC AAGGACCCGA ATGGATTTGG 
CCTATAGGTT ATTTTCTTCG AGCTCGATTG TATTTTGCTC CATTAATTGG TGGAAAAGAA 
GAATTATGCC GTACAATTGA ATCAACTGAA ACTATTATTT CTCGACATTT TATAGAAGCA 
TCTACAAATC ATTGGAGGGG TCTTCCAGAA CTTACTAACA AAGATGGGGA ATATTGCAAA 
GATAGTTGTA GATCGCAAGC TTGGAGTGCT GCTTCTATCC TAGAAGTTCT TTATGATTTA 
CATAAAATCA AACGGGAATT GCATTCTGAG GAGAATGAAA GAACGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006561267.1
CDS	281..4951
Translation	MSEHMSSTESHEFEQIRVLTLNNGEHLDGILYRLKKGWRVEFRLGASLLGRTVDLFINYPLGENKKFERHTYYLLEWINETANICLSLAGSFHYYLIDPNSEGMKPIASGYLLVDPELKIGEHGNDLPLDCIQCQTVLAKCLGSFSTWEDKLLVAKNSGYNMIHFTPIQELGYSKSSYSLSDQLKLNPSFNDDNKSITYDDIEKLINKMRNEWNMLSICDIVLNHTANESPFLVSHPECTYNCLNSPHLRPAYILDAALFELTVQVAAGEWEFKGIPLIVETEEHLNSIRHALHTYFLPLIKIHELYIIDINETIAEFLNLARNQMPQDIIESSKTSEDIKIIQDSKFRRLKATINMQLALQKYNIYRTDCFDEETRLKRCAEDLRKKLQELNNIIINEIQNHLNAAVENTIAGIRYFRVQVDGPKLKEISERNPLVPRYFTDYGTPKTLTEREATMYSDAGCYLMAHNGWVMNGDPLKNFADSDSNVYIRRELIAWGDSVKLRYGDKPEDCPFLWQHISTYVEQTAKIFDGIRLDNCHSTPIPVAEYMLDVARRIRPDLYVVAELFTNSDQKDNIFVNRLGITSLIREAMSAWDSHEEGRLVYRYGGEPVGAFLQSRKRPLVPSIAHALFLDLTHDNPSTVEKRSVFDLLPSAALVSMAACASGSNRGYDELVPHHIHVVDETRQYSFWTDDECLIDGIKFVGFKTGIIAAKKALNDLHYILGQQKFSQVFVDQMDNDIVAVTRHSPTTHDSVnullLIAFTAFKHPDPNANDLRRHVKPLRVEGVMEEIILEASLCHVEAKNDISPFHYSEKYVRDESYINGLSEYIVNLKEHIQICDSEILEKVDSGDPKITQLNFVNFQPGSVIAIRVSLHANIKPALAKLHNTISIITSQKSSDIRVIASRMDLIDLNKALYRCDQEERDETSNRFDVYNIPGYGPLVYAGLQGIISLLADIRPNNDLGHPMCINLRQGNWLIDYTWQRLKEDDGTKALGEWLEEATEPFKLIPRYLVPSYFDVIVVNVYMILLEQCYNLMSSFVKDGSTFVKILSLVSIQMGGVIKSAPLPDLSPNIDSPKPKIKQYNDESEQICMTLSAGLPHFTTGYMRNWGRDTFIALRGLLILTGRHTEARFIILGYAGTLRHGLIPNLLDKGKNARYNCRDALWWWLYTIQCYIQEVPNGLKILTDKVSRLFPTDDSPPLPAGQVDQSLHDVIQEALLVHFQGLCFRERNAGKQIDEHMTDRGFNNQIGVHPETGFIFGGNDANCGTWMDKMGSSEKAGNKGKPATPRDGSAVEIVGLSKSILSFLAELYKQNLFPYGSVQRKNRDGSVVTWSYKQWADKISLNFEKYFYINEIAKEGELNLELIHRRGIFKDSHGATQAWADYQLRPNFPVAMVVAPELFNPEHAWTALKKVEEILLGPLGMKTLDPADWAYNGYYDNSNDSGDTKLAHGWNYHQGPEWIWPIGYFLRARLYFAPLIGGKEELCRTIESTETIISRHFIEASTNHWRGLPELTNKDGEYCKDSCRSQAWSAASILEVLYDLHKIKRELHSEENERTN

Target ORF information:

RefSeq Version	XM_006561204.2
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera glycogen debranching enzyme (LOC411488), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006561204.2

ORF Insert Sequence:

ATGTCAGAAC ACATGTCATC AACTGAAAGT CACGAATTTG AACAAATAAG AGTTTTAACT 
CTAAATAATG GAGAACATCT TGATGGTATA TTATATAGAT TAAAGAAAGG ATGGAGAGTA 
GAATTTCGTT TAGGCGCTTC TCTTCTTGGA AGAACTGTTG ATCTTTTTAT AAATTATCCT 
TTAGGAGAGA ATAAAAAATT TGAAAGACAT ACATATTATC TCTTAGAATG GATTAATGAA 
ACAGCTAATA TATGTTTATC TCTAGCTGGT TCATTTCATT ATTATCTTAT AGATCCTAAT 
TCTGAAGGTA TGAAGCCTAT TGCTTCTGGA TATCTCCTTG TAGATCCAGA ACTTAAAATA 
GGGGAACATG GGAATGATTT ACCATTAGAT TGTATTCAAT GTCAAACTGT TCTTGCAAAA 
TGTTTAGGAT CATTTTCTAC CTGGGAAGAT AAATTACTAG TCGCTAAAAA TTCTGGATAT 
AACATGATTC ATTTTACACC AATTCAGGAA TTGGGATATT CTAAATCTTC TTATAGTCTT 
AGTGATCAAT TAAAATTAAA TCCTTCTTTT AATGATGATA ATAAATCAAT TACATATGAT 
GATATTGAAA AATTAATTAA TAAAATGCGC AATGAATGGA ATATGTTAAG CATCTGTGAT 
ATTGTTTTGA ATCATACAGC AAATGAAAGT CCTTTTTTGG TATCTCATCC AGAATGTACA 
TATAATTGTT TGAATAGTCC TCATTTACGT CCTGCATATA TTTTGGATGC TGCATTATTT 
GAATTAACAG TGCAAGTGGC TGCTGGAGAA TGGGAATTTA AAGGCATCCC TTTGATAGTT 
GAAACTGAAG AACATTTAAA TTCAATTCGT CATGCACTTC ACACATATTT TTTGCCGCTT 
ATAAAAATAC ACGAATTATA TATAATAGAT ATTAATGAAA CTATTGCTGA GTTTTTGAAT 
TTAGCACGAA ATCAAATGCC TCAAGACATC ATTGAATCAT CAAAAACATC AGAAGATATT 
AAAATAATAC AAGATTCAAA ATTTCGCAGA TTAAAAGCAA CTATAAATAT GCAACTTGCT 
TTACAAAAAT ATAACATATA TAGAACTGAT TGTTTTGATG AAGAGACTCG TTTGAAACGT 
TGTGCTGAAG ATTTAAGAAA AAAATTGCAG GAACTTAATA ATATAATCAT TAATGAAATA 
CAAAATCATT TAAATGCTGC AGTTGAAAAT ACAATTGCTG GTATAAGATA TTTTAGAGTG 
CAAGTTGATG GACCTAAACT TAAAGAAATA AGTGAAAGAA ATCCACTAGT TCCTAGATAT 
TTTACTGATT ATGGAACACC TAAAACATTA ACTGAAAGAG AAGCAACAAT GTATTCAGAT 
GCAGGATGTT ATCTTATGGC TCATAATGGA TGGGTAATGA ATGGCGATCC TTTAAAAAAT 
TTTGCAGATT CTGATTCTAA TGTTTATATA AGAAGAGAAC TTATTGCTTG GGGTGATAGT 
GTAAAATTGA GATATGGAGA TAAACCAGAA GATTGTCCTT TTCTATGGCA GCATATTAGT 
ACATATGTTG AACAAACAGC TAAAATTTTT GATGGTATTC GATTAGACAA TTGTCATTCA 
ACTCCTATTC CAGTTGCAGA GTATATGCTT GATGTTGCTC GTCGTATCCG TCCAGATTTG 
TACGTTGTTG CAGAATTATT TACAAATTCA GATCAAAAAG ATAATATATT TGTTAATCGT 
CTTGGTATCA CTTCTTTAAT TCGAGAAGCT ATGTCTGCAT GGGATAGCCA CGAAGAAGGT 
CGATTGGTAT ATCGATATGG AGGTGAACCA GTCGGTGCAT TTTTGCAATC GCGTAAACGA 
CCTTTAGTAC CAAGCATAGC ACATGCCCTT TTTTTAGATT TAACTCATGA TAATCCTAGC 
ACTGTGGAAA AACGCAGCGT ATTCGATTTA CTTCCAAGTG CTGCTCTTGT ATCAATGGCA 
GCATGTGCTA GTGGTAGTAA TCGTGGATAT GATGAATTAG TTCCTCATCA TATACATGTT 
GTAGATGAAA CAAGACAATA TTCATTTTGG ACAGATGATG AATGTTTGAT AGATGGTATC 
AAATTTGTAG GTTTTAAAAC TGGTATAATT GCTGCAAAGA AAGCATTAAA TGATTTGCAT 
TATATTCTTG GACAACAAAA ATTTTCTCAA GTATTTGTTG ATCAAATGGA TAATGATATA 
GTAGCTGTAA CAAGACACTC TCCAACAACT CATGATAGTG TAATNTTAAT TGCTTTTACT 
GCTTTTAAAC ATCCTGATCC TAATGCTAAT GATTTGAGAA GACATGTGAA ACCATTACGA 
GTGGAAGGTG TAATGGAAGA AATTATTTTG GAAGCATCCT TATGTCATGT AGAAGCAAAA 
AATGATATCT CACCTTTCCA TTATTCTGAA AAATATGTAC GAGATGAAAG TTATATAAAT 
GGTCTCTCAG AATATATTGT AAATCTTAAA GAACATATAC AAATTTGTGA TTCAGAAATT 
TTGGAAAAAG TTGATTCTGG TGATCCTAAA ATAACTCAAC TTAATTTTGT AAATTTTCAA 
CCAGGTTCTG TTATTGCTAT TCGTGTATCT CTTCATGCTA ATATAAAACC AGCTCTTGCA 
AAACTTCATA ATACTATTTC AATAATCACA TCCCAAAAAA GTTCAGACAT ACGCGTTATT 
GCTTCACGTA TGGATTTGAT AGATTTAAAT AAAGCTTTAT ATCGATGTGA TCAGGAAGAA 
CGCGATGAAA CTTCAAATAG ATTTGATGTA TATAATATTC CAGGTTATGG ACCACTTGTT 
TATGCAGGAT TGCAAGGTAT TATTTCTTTA TTAGCGGATA TTCGTCCTAA TAATGATTTA 
GGTCATCCAA TGTGTATTAA TCTCAGACAA GGTAATTGGT TAATAGATTA TACTTGGCAA 
CGGTTAAAAG AAGACGATGG AACAAAAGCA CTTGGAGAAT GGCTTGAAGA AGCTACTGAA 
CCATTTAAAT TAATACCTCG TTATTTAGTT CCTAGTTATT TTGATGTTAT AGTTGTAAAC 
GTTTATATGA TACTTCTCGA ACAATGCTAT AATCTAATGT CAAGCTTTGT GAAAGATGGT 
TCTACTTTTG TCAAGATCTT ATCTTTGGTT TCAATACAAA TGGGAGGAGT AATAAAATCA 
GCTCCATTAC CAGATTTGTC ACCAAATATA GATTCTCCTA AACCAAAGAT TAAACAATAT 
AATGACGAAA GTGAACAAAT ATGTATGACA TTATCTGCTG GTTTACCGCA TTTCACAACA 
GGATATATGA GAAATTGGGG AAGAGATACA TTTATTGCTT TAAGAGGACT ATTGATATTA 
ACTGGCAGAC ATACAGAAGC ACGATTTATT ATTCTTGGTT ATGCTGGAAC TTTACGACAT 
GGTCTTATAC CAAATTTACT TGATAAAGGA AAGAATGCTA GATATAACTG TCGAGATGCT 
TTATGGTGGT GGTTGTATAC AATTCAATGT TATATACAAG AAGTGCCAAA TGGTTTAAAA 
ATTTTAACAG ATAAAGTTTC AAGACTGTTT CCAACTGATG ATTCTCCTCC ATTACCCGCG 
GGACAAGTAG ATCAATCATT ACATGATGTT ATTCAAGAAG CTCTTTTAGT ACATTTTCAA 
GGACTTTGTT TTAGAGAAAG AAATGCTGGA AAACAGATTG ATGAACATAT GACAGATCGT 
GGTTTTAATA ATCAAATTGG AGTACATCCA GAAACAGGAT TTATATTTGG TGGAAATGAT 
GCAAATTGTG GTACGTGGAT GGATAAAATG GGATCTTCTG AAAAAGCTGG TAACAAAGGA 
AAACCAGCTA CTCCAAGGGA TGGTTCAGCT GTAGAAATAG TGGGTCTCAG TAAAAGTATT 
CTTAGTTTTT TAGCTGAATT ATATAAACAA AATCTTTTTC CTTATGGTAG TGTGCAAAGA 
AAAAATCGTG ATGGTTCTGT TGTAACATGG AGTTATAAAC AATGGGCAGA TAAAATTTCA 
TTAAATTTTG AAAAATATTT TTATATTAAT GAAATAGCAA AAGAAGGAGA ATTAAATTTA 
GAACTTATTC ATCGGCGTGG AATTTTTAAA GATAGTCATG GAGCAACACA AGCATGGGCT 
GATTATCAAC TACGACCTAA TTTTCCAGTT GCAATGGTTG TTGCTCCAGA GTTATTTAAT 
CCAGAACATG CATGGACAGC ATTAAAAAAA GTAGAAGAAA TTTTATTAGG GCCACTTGGA 
ATGAAAACAT TAGATCCTGC AGATTGGGCA TATAATGGAT ATTATGATAA TTCTAATGAT 
AGTGGAGACA CTAAATTAGC TCATGGTTGG AATTATCATC AAGGACCCGA ATGGATTTGG 
CCTATAGGTT ATTTTCTTCG AGCTCGATTG TATTTTGCTC CATTAATTGG TGGAAAAGAA 
GAATTATGCC GTACAATTGA ATCAACTGAA ACTATTATTT CTCGACATTT TATAGAAGCA 
TCTACAAATC ATTGGAGGGG TCTTCCAGAA CTTACTAACA AAGATGGGGA ATATTGCAAA 
GATAGTTGTA GATCGCAAGC TTGGAGTGCT GCTTCTATCC TAGAAGTTCT TTATGATTTA 
CATAAAATCA AACGGGAATT GCATTCTGAG GAGAATGAAA GAACGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC411488 ( XM_016915601.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_016771090.1 Apis mellifera glycogen debranching enzyme (LOC411488), transcript variant X4, mRNA.

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CloneID	OHd13286
Clone ID Related Accession (Same CDS sequence)	XM_006561204.2 , XM_016915601.1 , XM_006561203.2
Accession Version	XM_016915601.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1463932800000
Organism	Apis mellifera(honey bee)
Product	glycogen debranching enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007081.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis mellifera Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAC ACATGTCATC AACTGAAAGT CACGAATTTG AACAAATAAG AGTTTTAACT 
CTAAATAATG GAGAACATCT TGATGGTATA TTATATAGAT TAAAGAAAGG ATGGAGAGTA 
GAATTTCGTT TAGGCGCTTC TCTTCTTGGA AGAACTGTTG ATCTTTTTAT AAATTATCCT 
TTAGGAGAGA ATAAAAAATT TGAAAGACAT ACATATTATC TCTTAGAATG GATTAATGAA 
ACAGCTAATA TATGTTTATC TCTAGCTGGT TCATTTCATT ATTATCTTAT AGATCCTAAT 
TCTGAAGGTA TGAAGCCTAT TGCTTCTGGA TATCTCCTTG TAGATCCAGA ACTTAAAATA 
GGGGAACATG GGAATGATTT ACCATTAGAT TGTATTCAAT GTCAAACTGT TCTTGCAAAA 
TGTTTAGGAT CATTTTCTAC CTGGGAAGAT AAATTACTAG TCGCTAAAAA TTCTGGATAT 
AACATGATTC ATTTTACACC AATTCAGGAA TTGGGATATT CTAAATCTTC TTATAGTCTT 
AGTGATCAAT TAAAATTAAA TCCTTCTTTT AATGATGATA ATAAATCAAT TACATATGAT 
GATATTGAAA AATTAATTAA TAAAATGCGC AATGAATGGA ATATGTTAAG CATCTGTGAT 
ATTGTTTTGA ATCATACAGC AAATGAAAGT CCTTTTTTGG TATCTCATCC AGAATGTACA 
TATAATTGTT TGAATAGTCC TCATTTACGT CCTGCATATA TTTTGGATGC TGCATTATTT 
GAATTAACAG TGCAAGTGGC TGCTGGAGAA TGGGAATTTA AAGGCATCCC TTTGATAGTT 
GAAACTGAAG AACATTTAAA TTCAATTCGT CATGCACTTC ACACATATTT TTTGCCGCTT 
ATAAAAATAC ACGAATTATA TATAATAGAT ATTAATGAAA CTATTGCTGA GTTTTTGAAT 
TTAGCACGAA ATCAAATGCC TCAAGACATC ATTGAATCAT CAAAAACATC AGAAGATATT 
AAAATAATAC AAGATTCAAA ATTTCGCAGA TTAAAAGCAA CTATAAATAT GCAACTTGCT 
TTACAAAAAT ATAACATATA TAGAACTGAT TGTTTTGATG AAGAGACTCG TTTGAAACGT 
TGTGCTGAAG ATTTAAGAAA AAAATTGCAG GAACTTAATA ATATAATCAT TAATGAAATA 
CAAAATCATT TAAATGCTGC AGTTGAAAAT ACAATTGCTG GTATAAGATA TTTTAGAGTG 
CAAGTTGATG GACCTAAACT TAAAGAAATA AGTGAAAGAA ATCCACTAGT TCCTAGATAT 
TTTACTGATT ATGGAACACC TAAAACATTA ACTGAAAGAG AAGCAACAAT GTATTCAGAT 
GCAGGATGTT ATCTTATGGC TCATAATGGA TGGGTAATGA ATGGCGATCC TTTAAAAAAT 
TTTGCAGATT CTGATTCTAA TGTTTATATA AGAAGAGAAC TTATTGCTTG GGGTGATAGT 
GTAAAATTGA GATATGGAGA TAAACCAGAA GATTGTCCTT TTCTATGGCA GCATATTAGT 
ACATATGTTG AACAAACAGC TAAAATTTTT GATGGTATTC GATTAGACAA TTGTCATTCA 
ACTCCTATTC CAGTTGCAGA GTATATGCTT GATGTTGCTC GTCGTATCCG TCCAGATTTG 
TACGTTGTTG CAGAATTATT TACAAATTCA GATCAAAAAG ATAATATATT TGTTAATCGT 
CTTGGTATCA CTTCTTTAAT TCGAGAAGCT ATGTCTGCAT GGGATAGCCA CGAAGAAGGT 
CGATTGGTAT ATCGATATGG AGGTGAACCA GTCGGTGCAT TTTTGCAATC GCGTAAACGA 
CCTTTAGTAC CAAGCATAGC ACATGCCCTT TTTTTAGATT TAACTCATGA TAATCCTAGC 
ACTGTGGAAA AACGCAGCGT ATTCGATTTA CTTCCAAGTG CTGCTCTTGT ATCAATGGCA 
GCATGTGCTA GTGGTAGTAA TCGTGGATAT GATGAATTAG TTCCTCATCA TATACATGTT 
GTAGATGAAA CAAGACAATA TTCATTTTGG ACAGATGATG AATGTTTGAT AGATGGTATC 
AAATTTGTAG GTTTTAAAAC TGGTATAATT GCTGCAAAGA AAGCATTAAA TGATTTGCAT 
TATATTCTTG GACAACAAAA ATTTTCTCAA GTATTTGTTG ATCAAATGGA TAATGATATA 
GTAGCTGTAA CAAGACACTC TCCAACAACT CATGATAGTG TAATNTTAAT TGCTTTTACT 
GCTTTTAAAC ATCCTGATCC TAATGCTAAT GATTTGAGAA GACATGTGAA ACCATTACGA 
GTGGAAGGTG TAATGGAAGA AATTATTTTG GAAGCATCCT TATGTCATGT AGAAGCAAAA 
AATGATATCT CACCTTTCCA TTATTCTGAA AAATATGTAC GAGATGAAAG TTATATAAAT 
GGTCTCTCAG AATATATTGT AAATCTTAAA GAACATATAC AAATTTGTGA TTCAGAAATT 
TTGGAAAAAG TTGATTCTGG TGATCCTAAA ATAACTCAAC TTAATTTTGT AAATTTTCAA 
CCAGGTTCTG TTATTGCTAT TCGTGTATCT CTTCATGCTA ATATAAAACC AGCTCTTGCA 
AAACTTCATA ATACTATTTC AATAATCACA TCCCAAAAAA GTTCAGACAT ACGCGTTATT 
GCTTCACGTA TGGATTTGAT AGATTTAAAT AAAGCTTTAT ATCGATGTGA TCAGGAAGAA 
CGCGATGAAA CTTCAAATAG ATTTGATGTA TATAATATTC CAGGTTATGG ACCACTTGTT 
TATGCAGGAT TGCAAGGTAT TATTTCTTTA TTAGCGGATA TTCGTCCTAA TAATGATTTA 
GGTCATCCAA TGTGTATTAA TCTCAGACAA GGTAATTGGT TAATAGATTA TACTTGGCAA 
CGGTTAAAAG AAGACGATGG AACAAAAGCA CTTGGAGAAT GGCTTGAAGA AGCTACTGAA 
CCATTTAAAT TAATACCTCG TTATTTAGTT CCTAGTTATT TTGATGTTAT AGTTGTAAAC 
GTTTATATGA TACTTCTCGA ACAATGCTAT AATCTAATGT CAAGCTTTGT GAAAGATGGT 
TCTACTTTTG TCAAGATCTT ATCTTTGGTT TCAATACAAA TGGGAGGAGT AATAAAATCA 
GCTCCATTAC CAGATTTGTC ACCAAATATA GATTCTCCTA AACCAAAGAT TAAACAATAT 
AATGACGAAA GTGAACAAAT ATGTATGACA TTATCTGCTG GTTTACCGCA TTTCACAACA 
GGATATATGA GAAATTGGGG AAGAGATACA TTTATTGCTT TAAGAGGACT ATTGATATTA 
ACTGGCAGAC ATACAGAAGC ACGATTTATT ATTCTTGGTT ATGCTGGAAC TTTACGACAT 
GGTCTTATAC CAAATTTACT TGATAAAGGA AAGAATGCTA GATATAACTG TCGAGATGCT 
TTATGGTGGT GGTTGTATAC AATTCAATGT TATATACAAG AAGTGCCAAA TGGTTTAAAA 
ATTTTAACAG ATAAAGTTTC AAGACTGTTT CCAACTGATG ATTCTCCTCC ATTACCCGCG 
GGACAAGTAG ATCAATCATT ACATGATGTT ATTCAAGAAG CTCTTTTAGT ACATTTTCAA 
GGACTTTGTT TTAGAGAAAG AAATGCTGGA AAACAGATTG ATGAACATAT GACAGATCGT 
GGTTTTAATA ATCAAATTGG AGTACATCCA GAAACAGGAT TTATATTTGG TGGAAATGAT 
GCAAATTGTG GTACGTGGAT GGATAAAATG GGATCTTCTG AAAAAGCTGG TAACAAAGGA 
AAACCAGCTA CTCCAAGGGA TGGTTCAGCT GTAGAAATAG TGGGTCTCAG TAAAAGTATT 
CTTAGTTTTT TAGCTGAATT ATATAAACAA AATCTTTTTC CTTATGGTAG TGTGCAAAGA 
AAAAATCGTG ATGGTTCTGT TGTAACATGG AGTTATAAAC AATGGGCAGA TAAAATTTCA 
TTAAATTTTG AAAAATATTT TTATATTAAT GAAATAGCAA AAGAAGGAGA ATTAAATTTA 
GAACTTATTC ATCGGCGTGG AATTTTTAAA GATAGTCATG GAGCAACACA AGCATGGGCT 
GATTATCAAC TACGACCTAA TTTTCCAGTT GCAATGGTTG TTGCTCCAGA GTTATTTAAT 
CCAGAACATG CATGGACAGC ATTAAAAAAA GTAGAAGAAA TTTTATTAGG GCCACTTGGA 
ATGAAAACAT TAGATCCTGC AGATTGGGCA TATAATGGAT ATTATGATAA TTCTAATGAT 
AGTGGAGACA CTAAATTAGC TCATGGTTGG AATTATCATC AAGGACCCGA ATGGATTTGG 
CCTATAGGTT ATTTTCTTCG AGCTCGATTG TATTTTGCTC CATTAATTGG TGGAAAAGAA 
GAATTATGCC GTACAATTGA ATCAACTGAA ACTATTATTT CTCGACATTT TATAGAAGCA 
TCTACAAATC ATTGGAGGGG TCTTCCAGAA CTTACTAACA AAGATGGGGA ATATTGCAAA 
GATAGTTGTA GATCGCAAGC TTGGAGTGCT GCTTCTATCC TAGAAGTTCT TTATGATTTA 
CATAAAATCA AACGGGAATT GCATTCTGAG GAGAATGAAA GAACGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016771090.1
CDS	76..4746
Translation	MSEHMSSTESHEFEQIRVLTLNNGEHLDGILYRLKKGWRVEFRLGASLLGRTVDLFINYPLGENKKFERHTYYLLEWINETANICLSLAGSFHYYLIDPNSEGMKPIASGYLLVDPELKIGEHGNDLPLDCIQCQTVLAKCLGSFSTWEDKLLVAKNSGYNMIHFTPIQELGYSKSSYSLSDQLKLNPSFNDDNKSITYDDIEKLINKMRNEWNMLSICDIVLNHTANESPFLVSHPECTYNCLNSPHLRPAYILDAALFELTVQVAAGEWEFKGIPLIVETEEHLNSIRHALHTYFLPLIKIHELYIIDINETIAEFLNLARNQMPQDIIESSKTSEDIKIIQDSKFRRLKATINMQLALQKYNIYRTDCFDEETRLKRCAEDLRKKLQELNNIIINEIQNHLNAAVENTIAGIRYFRVQVDGPKLKEISERNPLVPRYFTDYGTPKTLTEREATMYSDAGCYLMAHNGWVMNGDPLKNFADSDSNVYIRRELIAWGDSVKLRYGDKPEDCPFLWQHISTYVEQTAKIFDGIRLDNCHSTPIPVAEYMLDVARRIRPDLYVVAELFTNSDQKDNIFVNRLGITSLIREAMSAWDSHEEGRLVYRYGGEPVGAFLQSRKRPLVPSIAHALFLDLTHDNPSTVEKRSVFDLLPSAALVSMAACASGSNRGYDELVPHHIHVVDETRQYSFWTDDECLIDGIKFVGFKTGIIAAKKALNDLHYILGQQKFSQVFVDQMDNDIVAVTRHSPTTHDSVnullLIAFTAFKHPDPNANDLRRHVKPLRVEGVMEEIILEASLCHVEAKNDISPFHYSEKYVRDESYINGLSEYIVNLKEHIQICDSEILEKVDSGDPKITQLNFVNFQPGSVIAIRVSLHANIKPALAKLHNTISIITSQKSSDIRVIASRMDLIDLNKALYRCDQEERDETSNRFDVYNIPGYGPLVYAGLQGIISLLADIRPNNDLGHPMCINLRQGNWLIDYTWQRLKEDDGTKALGEWLEEATEPFKLIPRYLVPSYFDVIVVNVYMILLEQCYNLMSSFVKDGSTFVKILSLVSIQMGGVIKSAPLPDLSPNIDSPKPKIKQYNDESEQICMTLSAGLPHFTTGYMRNWGRDTFIALRGLLILTGRHTEARFIILGYAGTLRHGLIPNLLDKGKNARYNCRDALWWWLYTIQCYIQEVPNGLKILTDKVSRLFPTDDSPPLPAGQVDQSLHDVIQEALLVHFQGLCFRERNAGKQIDEHMTDRGFNNQIGVHPETGFIFGGNDANCGTWMDKMGSSEKAGNKGKPATPRDGSAVEIVGLSKSILSFLAELYKQNLFPYGSVQRKNRDGSVVTWSYKQWADKISLNFEKYFYINEIAKEGELNLELIHRRGIFKDSHGATQAWADYQLRPNFPVAMVVAPELFNPEHAWTALKKVEEILLGPLGMKTLDPADWAYNGYYDNSNDSGDTKLAHGWNYHQGPEWIWPIGYFLRARLYFAPLIGGKEELCRTIESTETIISRHFIEASTNHWRGLPELTNKDGEYCKDSCRSQAWSAASILEVLYDLHKIKRELHSEENERTN

Target ORF information:

RefSeq Version	XM_016915601.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera glycogen debranching enzyme (LOC411488), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016915601.1

ORF Insert Sequence:

ATGTCAGAAC ACATGTCATC AACTGAAAGT CACGAATTTG AACAAATAAG AGTTTTAACT 
CTAAATAATG GAGAACATCT TGATGGTATA TTATATAGAT TAAAGAAAGG ATGGAGAGTA 
GAATTTCGTT TAGGCGCTTC TCTTCTTGGA AGAACTGTTG ATCTTTTTAT AAATTATCCT 
TTAGGAGAGA ATAAAAAATT TGAAAGACAT ACATATTATC TCTTAGAATG GATTAATGAA 
ACAGCTAATA TATGTTTATC TCTAGCTGGT TCATTTCATT ATTATCTTAT AGATCCTAAT 
TCTGAAGGTA TGAAGCCTAT TGCTTCTGGA TATCTCCTTG TAGATCCAGA ACTTAAAATA 
GGGGAACATG GGAATGATTT ACCATTAGAT TGTATTCAAT GTCAAACTGT TCTTGCAAAA 
TGTTTAGGAT CATTTTCTAC CTGGGAAGAT AAATTACTAG TCGCTAAAAA TTCTGGATAT 
AACATGATTC ATTTTACACC AATTCAGGAA TTGGGATATT CTAAATCTTC TTATAGTCTT 
AGTGATCAAT TAAAATTAAA TCCTTCTTTT AATGATGATA ATAAATCAAT TACATATGAT 
GATATTGAAA AATTAATTAA TAAAATGCGC AATGAATGGA ATATGTTAAG CATCTGTGAT 
ATTGTTTTGA ATCATACAGC AAATGAAAGT CCTTTTTTGG TATCTCATCC AGAATGTACA 
TATAATTGTT TGAATAGTCC TCATTTACGT CCTGCATATA TTTTGGATGC TGCATTATTT 
GAATTAACAG TGCAAGTGGC TGCTGGAGAA TGGGAATTTA AAGGCATCCC TTTGATAGTT 
GAAACTGAAG AACATTTAAA TTCAATTCGT CATGCACTTC ACACATATTT TTTGCCGCTT 
ATAAAAATAC ACGAATTATA TATAATAGAT ATTAATGAAA CTATTGCTGA GTTTTTGAAT 
TTAGCACGAA ATCAAATGCC TCAAGACATC ATTGAATCAT CAAAAACATC AGAAGATATT 
AAAATAATAC AAGATTCAAA ATTTCGCAGA TTAAAAGCAA CTATAAATAT GCAACTTGCT 
TTACAAAAAT ATAACATATA TAGAACTGAT TGTTTTGATG AAGAGACTCG TTTGAAACGT 
TGTGCTGAAG ATTTAAGAAA AAAATTGCAG GAACTTAATA ATATAATCAT TAATGAAATA 
CAAAATCATT TAAATGCTGC AGTTGAAAAT ACAATTGCTG GTATAAGATA TTTTAGAGTG 
CAAGTTGATG GACCTAAACT TAAAGAAATA AGTGAAAGAA ATCCACTAGT TCCTAGATAT 
TTTACTGATT ATGGAACACC TAAAACATTA ACTGAAAGAG AAGCAACAAT GTATTCAGAT 
GCAGGATGTT ATCTTATGGC TCATAATGGA TGGGTAATGA ATGGCGATCC TTTAAAAAAT 
TTTGCAGATT CTGATTCTAA TGTTTATATA AGAAGAGAAC TTATTGCTTG GGGTGATAGT 
GTAAAATTGA GATATGGAGA TAAACCAGAA GATTGTCCTT TTCTATGGCA GCATATTAGT 
ACATATGTTG AACAAACAGC TAAAATTTTT GATGGTATTC GATTAGACAA TTGTCATTCA 
ACTCCTATTC CAGTTGCAGA GTATATGCTT GATGTTGCTC GTCGTATCCG TCCAGATTTG 
TACGTTGTTG CAGAATTATT TACAAATTCA GATCAAAAAG ATAATATATT TGTTAATCGT 
CTTGGTATCA CTTCTTTAAT TCGAGAAGCT ATGTCTGCAT GGGATAGCCA CGAAGAAGGT 
CGATTGGTAT ATCGATATGG AGGTGAACCA GTCGGTGCAT TTTTGCAATC GCGTAAACGA 
CCTTTAGTAC CAAGCATAGC ACATGCCCTT TTTTTAGATT TAACTCATGA TAATCCTAGC 
ACTGTGGAAA AACGCAGCGT ATTCGATTTA CTTCCAAGTG CTGCTCTTGT ATCAATGGCA 
GCATGTGCTA GTGGTAGTAA TCGTGGATAT GATGAATTAG TTCCTCATCA TATACATGTT 
GTAGATGAAA CAAGACAATA TTCATTTTGG ACAGATGATG AATGTTTGAT AGATGGTATC 
AAATTTGTAG GTTTTAAAAC TGGTATAATT GCTGCAAAGA AAGCATTAAA TGATTTGCAT 
TATATTCTTG GACAACAAAA ATTTTCTCAA GTATTTGTTG ATCAAATGGA TAATGATATA 
GTAGCTGTAA CAAGACACTC TCCAACAACT CATGATAGTG TAATNTTAAT TGCTTTTACT 
GCTTTTAAAC ATCCTGATCC TAATGCTAAT GATTTGAGAA GACATGTGAA ACCATTACGA 
GTGGAAGGTG TAATGGAAGA AATTATTTTG GAAGCATCCT TATGTCATGT AGAAGCAAAA 
AATGATATCT CACCTTTCCA TTATTCTGAA AAATATGTAC GAGATGAAAG TTATATAAAT 
GGTCTCTCAG AATATATTGT AAATCTTAAA GAACATATAC AAATTTGTGA TTCAGAAATT 
TTGGAAAAAG TTGATTCTGG TGATCCTAAA ATAACTCAAC TTAATTTTGT AAATTTTCAA 
CCAGGTTCTG TTATTGCTAT TCGTGTATCT CTTCATGCTA ATATAAAACC AGCTCTTGCA 
AAACTTCATA ATACTATTTC AATAATCACA TCCCAAAAAA GTTCAGACAT ACGCGTTATT 
GCTTCACGTA TGGATTTGAT AGATTTAAAT AAAGCTTTAT ATCGATGTGA TCAGGAAGAA 
CGCGATGAAA CTTCAAATAG ATTTGATGTA TATAATATTC CAGGTTATGG ACCACTTGTT 
TATGCAGGAT TGCAAGGTAT TATTTCTTTA TTAGCGGATA TTCGTCCTAA TAATGATTTA 
GGTCATCCAA TGTGTATTAA TCTCAGACAA GGTAATTGGT TAATAGATTA TACTTGGCAA 
CGGTTAAAAG AAGACGATGG AACAAAAGCA CTTGGAGAAT GGCTTGAAGA AGCTACTGAA 
CCATTTAAAT TAATACCTCG TTATTTAGTT CCTAGTTATT TTGATGTTAT AGTTGTAAAC 
GTTTATATGA TACTTCTCGA ACAATGCTAT AATCTAATGT CAAGCTTTGT GAAAGATGGT 
TCTACTTTTG TCAAGATCTT ATCTTTGGTT TCAATACAAA TGGGAGGAGT AATAAAATCA 
GCTCCATTAC CAGATTTGTC ACCAAATATA GATTCTCCTA AACCAAAGAT TAAACAATAT 
AATGACGAAA GTGAACAAAT ATGTATGACA TTATCTGCTG GTTTACCGCA TTTCACAACA 
GGATATATGA GAAATTGGGG AAGAGATACA TTTATTGCTT TAAGAGGACT ATTGATATTA 
ACTGGCAGAC ATACAGAAGC ACGATTTATT ATTCTTGGTT ATGCTGGAAC TTTACGACAT 
GGTCTTATAC CAAATTTACT TGATAAAGGA AAGAATGCTA GATATAACTG TCGAGATGCT 
TTATGGTGGT GGTTGTATAC AATTCAATGT TATATACAAG AAGTGCCAAA TGGTTTAAAA 
ATTTTAACAG ATAAAGTTTC AAGACTGTTT CCAACTGATG ATTCTCCTCC ATTACCCGCG 
GGACAAGTAG ATCAATCATT ACATGATGTT ATTCAAGAAG CTCTTTTAGT ACATTTTCAA 
GGACTTTGTT TTAGAGAAAG AAATGCTGGA AAACAGATTG ATGAACATAT GACAGATCGT 
GGTTTTAATA ATCAAATTGG AGTACATCCA GAAACAGGAT TTATATTTGG TGGAAATGAT 
GCAAATTGTG GTACGTGGAT GGATAAAATG GGATCTTCTG AAAAAGCTGG TAACAAAGGA 
AAACCAGCTA CTCCAAGGGA TGGTTCAGCT GTAGAAATAG TGGGTCTCAG TAAAAGTATT 
CTTAGTTTTT TAGCTGAATT ATATAAACAA AATCTTTTTC CTTATGGTAG TGTGCAAAGA 
AAAAATCGTG ATGGTTCTGT TGTAACATGG AGTTATAAAC AATGGGCAGA TAAAATTTCA 
TTAAATTTTG AAAAATATTT TTATATTAAT GAAATAGCAA AAGAAGGAGA ATTAAATTTA 
GAACTTATTC ATCGGCGTGG AATTTTTAAA GATAGTCATG GAGCAACACA AGCATGGGCT 
GATTATCAAC TACGACCTAA TTTTCCAGTT GCAATGGTTG TTGCTCCAGA GTTATTTAAT 
CCAGAACATG CATGGACAGC ATTAAAAAAA GTAGAAGAAA TTTTATTAGG GCCACTTGGA 
ATGAAAACAT TAGATCCTGC AGATTGGGCA TATAATGGAT ATTATGATAA TTCTAATGAT 
AGTGGAGACA CTAAATTAGC TCATGGTTGG AATTATCATC AAGGACCCGA ATGGATTTGG 
CCTATAGGTT ATTTTCTTCG AGCTCGATTG TATTTTGCTC CATTAATTGG TGGAAAAGAA 
GAATTATGCC GTACAATTGA ATCAACTGAA ACTATTATTT CTCGACATTT TATAGAAGCA 
TCTACAAATC ATTGGAGGGG TCTTCCAGAA CTTACTAACA AAGATGGGGA ATATTGCAAA 
GATAGTTGTA GATCGCAAGC TTGGAGTGCT GCTTCTATCC TAGAAGTTCT TTATGATTTA 
CATAAAATCA AACGGGAATT GCATTCTGAG GAGAATGAAA GAACGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC411488 ( XM_006561203.2 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_006561266.1 Apis mellifera glycogen debranching enzyme (LOC411488), transcript variant X3, mRNA.

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CloneID	OHd13286
Clone ID Related Accession (Same CDS sequence)	XM_006561204.2 , XM_016915601.1 , XM_006561203.2
Accession Version	XM_006561203.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1463932800000
Organism	Apis mellifera(honey bee)
Product	glycogen debranching enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007081.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 24, 2016 this sequence version replaced XM_006561203.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis mellifera Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAC ACATGTCATC AACTGAAAGT CACGAATTTG AACAAATAAG AGTTTTAACT 
CTAAATAATG GAGAACATCT TGATGGTATA TTATATAGAT TAAAGAAAGG ATGGAGAGTA 
GAATTTCGTT TAGGCGCTTC TCTTCTTGGA AGAACTGTTG ATCTTTTTAT AAATTATCCT 
TTAGGAGAGA ATAAAAAATT TGAAAGACAT ACATATTATC TCTTAGAATG GATTAATGAA 
ACAGCTAATA TATGTTTATC TCTAGCTGGT TCATTTCATT ATTATCTTAT AGATCCTAAT 
TCTGAAGGTA TGAAGCCTAT TGCTTCTGGA TATCTCCTTG TAGATCCAGA ACTTAAAATA 
GGGGAACATG GGAATGATTT ACCATTAGAT TGTATTCAAT GTCAAACTGT TCTTGCAAAA 
TGTTTAGGAT CATTTTCTAC CTGGGAAGAT AAATTACTAG TCGCTAAAAA TTCTGGATAT 
AACATGATTC ATTTTACACC AATTCAGGAA TTGGGATATT CTAAATCTTC TTATAGTCTT 
AGTGATCAAT TAAAATTAAA TCCTTCTTTT AATGATGATA ATAAATCAAT TACATATGAT 
GATATTGAAA AATTAATTAA TAAAATGCGC AATGAATGGA ATATGTTAAG CATCTGTGAT 
ATTGTTTTGA ATCATACAGC AAATGAAAGT CCTTTTTTGG TATCTCATCC AGAATGTACA 
TATAATTGTT TGAATAGTCC TCATTTACGT CCTGCATATA TTTTGGATGC TGCATTATTT 
GAATTAACAG TGCAAGTGGC TGCTGGAGAA TGGGAATTTA AAGGCATCCC TTTGATAGTT 
GAAACTGAAG AACATTTAAA TTCAATTCGT CATGCACTTC ACACATATTT TTTGCCGCTT 
ATAAAAATAC ACGAATTATA TATAATAGAT ATTAATGAAA CTATTGCTGA GTTTTTGAAT 
TTAGCACGAA ATCAAATGCC TCAAGACATC ATTGAATCAT CAAAAACATC AGAAGATATT 
AAAATAATAC AAGATTCAAA ATTTCGCAGA TTAAAAGCAA CTATAAATAT GCAACTTGCT 
TTACAAAAAT ATAACATATA TAGAACTGAT TGTTTTGATG AAGAGACTCG TTTGAAACGT 
TGTGCTGAAG ATTTAAGAAA AAAATTGCAG GAACTTAATA ATATAATCAT TAATGAAATA 
CAAAATCATT TAAATGCTGC AGTTGAAAAT ACAATTGCTG GTATAAGATA TTTTAGAGTG 
CAAGTTGATG GACCTAAACT TAAAGAAATA AGTGAAAGAA ATCCACTAGT TCCTAGATAT 
TTTACTGATT ATGGAACACC TAAAACATTA ACTGAAAGAG AAGCAACAAT GTATTCAGAT 
GCAGGATGTT ATCTTATGGC TCATAATGGA TGGGTAATGA ATGGCGATCC TTTAAAAAAT 
TTTGCAGATT CTGATTCTAA TGTTTATATA AGAAGAGAAC TTATTGCTTG GGGTGATAGT 
GTAAAATTGA GATATGGAGA TAAACCAGAA GATTGTCCTT TTCTATGGCA GCATATTAGT 
ACATATGTTG AACAAACAGC TAAAATTTTT GATGGTATTC GATTAGACAA TTGTCATTCA 
ACTCCTATTC CAGTTGCAGA GTATATGCTT GATGTTGCTC GTCGTATCCG TCCAGATTTG 
TACGTTGTTG CAGAATTATT TACAAATTCA GATCAAAAAG ATAATATATT TGTTAATCGT 
CTTGGTATCA CTTCTTTAAT TCGAGAAGCT ATGTCTGCAT GGGATAGCCA CGAAGAAGGT 
CGATTGGTAT ATCGATATGG AGGTGAACCA GTCGGTGCAT TTTTGCAATC GCGTAAACGA 
CCTTTAGTAC CAAGCATAGC ACATGCCCTT TTTTTAGATT TAACTCATGA TAATCCTAGC 
ACTGTGGAAA AACGCAGCGT ATTCGATTTA CTTCCAAGTG CTGCTCTTGT ATCAATGGCA 
GCATGTGCTA GTGGTAGTAA TCGTGGATAT GATGAATTAG TTCCTCATCA TATACATGTT 
GTAGATGAAA CAAGACAATA TTCATTTTGG ACAGATGATG AATGTTTGAT AGATGGTATC 
AAATTTGTAG GTTTTAAAAC TGGTATAATT GCTGCAAAGA AAGCATTAAA TGATTTGCAT 
TATATTCTTG GACAACAAAA ATTTTCTCAA GTATTTGTTG ATCAAATGGA TAATGATATA 
GTAGCTGTAA CAAGACACTC TCCAACAACT CATGATAGTG TAATNTTAAT TGCTTTTACT 
GCTTTTAAAC ATCCTGATCC TAATGCTAAT GATTTGAGAA GACATGTGAA ACCATTACGA 
GTGGAAGGTG TAATGGAAGA AATTATTTTG GAAGCATCCT TATGTCATGT AGAAGCAAAA 
AATGATATCT CACCTTTCCA TTATTCTGAA AAATATGTAC GAGATGAAAG TTATATAAAT 
GGTCTCTCAG AATATATTGT AAATCTTAAA GAACATATAC AAATTTGTGA TTCAGAAATT 
TTGGAAAAAG TTGATTCTGG TGATCCTAAA ATAACTCAAC TTAATTTTGT AAATTTTCAA 
CCAGGTTCTG TTATTGCTAT TCGTGTATCT CTTCATGCTA ATATAAAACC AGCTCTTGCA 
AAACTTCATA ATACTATTTC AATAATCACA TCCCAAAAAA GTTCAGACAT ACGCGTTATT 
GCTTCACGTA TGGATTTGAT AGATTTAAAT AAAGCTTTAT ATCGATGTGA TCAGGAAGAA 
CGCGATGAAA CTTCAAATAG ATTTGATGTA TATAATATTC CAGGTTATGG ACCACTTGTT 
TATGCAGGAT TGCAAGGTAT TATTTCTTTA TTAGCGGATA TTCGTCCTAA TAATGATTTA 
GGTCATCCAA TGTGTATTAA TCTCAGACAA GGTAATTGGT TAATAGATTA TACTTGGCAA 
CGGTTAAAAG AAGACGATGG AACAAAAGCA CTTGGAGAAT GGCTTGAAGA AGCTACTGAA 
CCATTTAAAT TAATACCTCG TTATTTAGTT CCTAGTTATT TTGATGTTAT AGTTGTAAAC 
GTTTATATGA TACTTCTCGA ACAATGCTAT AATCTAATGT CAAGCTTTGT GAAAGATGGT 
TCTACTTTTG TCAAGATCTT ATCTTTGGTT TCAATACAAA TGGGAGGAGT AATAAAATCA 
GCTCCATTAC CAGATTTGTC ACCAAATATA GATTCTCCTA AACCAAAGAT TAAACAATAT 
AATGACGAAA GTGAACAAAT ATGTATGACA TTATCTGCTG GTTTACCGCA TTTCACAACA 
GGATATATGA GAAATTGGGG AAGAGATACA TTTATTGCTT TAAGAGGACT ATTGATATTA 
ACTGGCAGAC ATACAGAAGC ACGATTTATT ATTCTTGGTT ATGCTGGAAC TTTACGACAT 
GGTCTTATAC CAAATTTACT TGATAAAGGA AAGAATGCTA GATATAACTG TCGAGATGCT 
TTATGGTGGT GGTTGTATAC AATTCAATGT TATATACAAG AAGTGCCAAA TGGTTTAAAA 
ATTTTAACAG ATAAAGTTTC AAGACTGTTT CCAACTGATG ATTCTCCTCC ATTACCCGCG 
GGACAAGTAG ATCAATCATT ACATGATGTT ATTCAAGAAG CTCTTTTAGT ACATTTTCAA 
GGACTTTGTT TTAGAGAAAG AAATGCTGGA AAACAGATTG ATGAACATAT GACAGATCGT 
GGTTTTAATA ATCAAATTGG AGTACATCCA GAAACAGGAT TTATATTTGG TGGAAATGAT 
GCAAATTGTG GTACGTGGAT GGATAAAATG GGATCTTCTG AAAAAGCTGG TAACAAAGGA 
AAACCAGCTA CTCCAAGGGA TGGTTCAGCT GTAGAAATAG TGGGTCTCAG TAAAAGTATT 
CTTAGTTTTT TAGCTGAATT ATATAAACAA AATCTTTTTC CTTATGGTAG TGTGCAAAGA 
AAAAATCGTG ATGGTTCTGT TGTAACATGG AGTTATAAAC AATGGGCAGA TAAAATTTCA 
TTAAATTTTG AAAAATATTT TTATATTAAT GAAATAGCAA AAGAAGGAGA ATTAAATTTA 
GAACTTATTC ATCGGCGTGG AATTTTTAAA GATAGTCATG GAGCAACACA AGCATGGGCT 
GATTATCAAC TACGACCTAA TTTTCCAGTT GCAATGGTTG TTGCTCCAGA GTTATTTAAT 
CCAGAACATG CATGGACAGC ATTAAAAAAA GTAGAAGAAA TTTTATTAGG GCCACTTGGA 
ATGAAAACAT TAGATCCTGC AGATTGGGCA TATAATGGAT ATTATGATAA TTCTAATGAT 
AGTGGAGACA CTAAATTAGC TCATGGTTGG AATTATCATC AAGGACCCGA ATGGATTTGG 
CCTATAGGTT ATTTTCTTCG AGCTCGATTG TATTTTGCTC CATTAATTGG TGGAAAAGAA 
GAATTATGCC GTACAATTGA ATCAACTGAA ACTATTATTT CTCGACATTT TATAGAAGCA 
TCTACAAATC ATTGGAGGGG TCTTCCAGAA CTTACTAACA AAGATGGGGA ATATTGCAAA 
GATAGTTGTA GATCGCAAGC TTGGAGTGCT GCTTCTATCC TAGAAGTTCT TTATGATTTA 
CATAAAATCA AACGGGAATT GCATTCTGAG GAGAATGAAA GAACGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006561266.1
CDS	202..4872
Translation	MSEHMSSTESHEFEQIRVLTLNNGEHLDGILYRLKKGWRVEFRLGASLLGRTVDLFINYPLGENKKFERHTYYLLEWINETANICLSLAGSFHYYLIDPNSEGMKPIASGYLLVDPELKIGEHGNDLPLDCIQCQTVLAKCLGSFSTWEDKLLVAKNSGYNMIHFTPIQELGYSKSSYSLSDQLKLNPSFNDDNKSITYDDIEKLINKMRNEWNMLSICDIVLNHTANESPFLVSHPECTYNCLNSPHLRPAYILDAALFELTVQVAAGEWEFKGIPLIVETEEHLNSIRHALHTYFLPLIKIHELYIIDINETIAEFLNLARNQMPQDIIESSKTSEDIKIIQDSKFRRLKATINMQLALQKYNIYRTDCFDEETRLKRCAEDLRKKLQELNNIIINEIQNHLNAAVENTIAGIRYFRVQVDGPKLKEISERNPLVPRYFTDYGTPKTLTEREATMYSDAGCYLMAHNGWVMNGDPLKNFADSDSNVYIRRELIAWGDSVKLRYGDKPEDCPFLWQHISTYVEQTAKIFDGIRLDNCHSTPIPVAEYMLDVARRIRPDLYVVAELFTNSDQKDNIFVNRLGITSLIREAMSAWDSHEEGRLVYRYGGEPVGAFLQSRKRPLVPSIAHALFLDLTHDNPSTVEKRSVFDLLPSAALVSMAACASGSNRGYDELVPHHIHVVDETRQYSFWTDDECLIDGIKFVGFKTGIIAAKKALNDLHYILGQQKFSQVFVDQMDNDIVAVTRHSPTTHDSVnullLIAFTAFKHPDPNANDLRRHVKPLRVEGVMEEIILEASLCHVEAKNDISPFHYSEKYVRDESYINGLSEYIVNLKEHIQICDSEILEKVDSGDPKITQLNFVNFQPGSVIAIRVSLHANIKPALAKLHNTISIITSQKSSDIRVIASRMDLIDLNKALYRCDQEERDETSNRFDVYNIPGYGPLVYAGLQGIISLLADIRPNNDLGHPMCINLRQGNWLIDYTWQRLKEDDGTKALGEWLEEATEPFKLIPRYLVPSYFDVIVVNVYMILLEQCYNLMSSFVKDGSTFVKILSLVSIQMGGVIKSAPLPDLSPNIDSPKPKIKQYNDESEQICMTLSAGLPHFTTGYMRNWGRDTFIALRGLLILTGRHTEARFIILGYAGTLRHGLIPNLLDKGKNARYNCRDALWWWLYTIQCYIQEVPNGLKILTDKVSRLFPTDDSPPLPAGQVDQSLHDVIQEALLVHFQGLCFRERNAGKQIDEHMTDRGFNNQIGVHPETGFIFGGNDANCGTWMDKMGSSEKAGNKGKPATPRDGSAVEIVGLSKSILSFLAELYKQNLFPYGSVQRKNRDGSVVTWSYKQWADKISLNFEKYFYINEIAKEGELNLELIHRRGIFKDSHGATQAWADYQLRPNFPVAMVVAPELFNPEHAWTALKKVEEILLGPLGMKTLDPADWAYNGYYDNSNDSGDTKLAHGWNYHQGPEWIWPIGYFLRARLYFAPLIGGKEELCRTIESTETIISRHFIEASTNHWRGLPELTNKDGEYCKDSCRSQAWSAASILEVLYDLHKIKRELHSEENERTN

Target ORF information:

RefSeq Version	XM_006561203.2
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera glycogen debranching enzyme (LOC411488), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006561203.2

ORF Insert Sequence:

ATGTCAGAAC ACATGTCATC AACTGAAAGT CACGAATTTG AACAAATAAG AGTTTTAACT 
CTAAATAATG GAGAACATCT TGATGGTATA TTATATAGAT TAAAGAAAGG ATGGAGAGTA 
GAATTTCGTT TAGGCGCTTC TCTTCTTGGA AGAACTGTTG ATCTTTTTAT AAATTATCCT 
TTAGGAGAGA ATAAAAAATT TGAAAGACAT ACATATTATC TCTTAGAATG GATTAATGAA 
ACAGCTAATA TATGTTTATC TCTAGCTGGT TCATTTCATT ATTATCTTAT AGATCCTAAT 
TCTGAAGGTA TGAAGCCTAT TGCTTCTGGA TATCTCCTTG TAGATCCAGA ACTTAAAATA 
GGGGAACATG GGAATGATTT ACCATTAGAT TGTATTCAAT GTCAAACTGT TCTTGCAAAA 
TGTTTAGGAT CATTTTCTAC CTGGGAAGAT AAATTACTAG TCGCTAAAAA TTCTGGATAT 
AACATGATTC ATTTTACACC AATTCAGGAA TTGGGATATT CTAAATCTTC TTATAGTCTT 
AGTGATCAAT TAAAATTAAA TCCTTCTTTT AATGATGATA ATAAATCAAT TACATATGAT 
GATATTGAAA AATTAATTAA TAAAATGCGC AATGAATGGA ATATGTTAAG CATCTGTGAT 
ATTGTTTTGA ATCATACAGC AAATGAAAGT CCTTTTTTGG TATCTCATCC AGAATGTACA 
TATAATTGTT TGAATAGTCC TCATTTACGT CCTGCATATA TTTTGGATGC TGCATTATTT 
GAATTAACAG TGCAAGTGGC TGCTGGAGAA TGGGAATTTA AAGGCATCCC TTTGATAGTT 
GAAACTGAAG AACATTTAAA TTCAATTCGT CATGCACTTC ACACATATTT TTTGCCGCTT 
ATAAAAATAC ACGAATTATA TATAATAGAT ATTAATGAAA CTATTGCTGA GTTTTTGAAT 
TTAGCACGAA ATCAAATGCC TCAAGACATC ATTGAATCAT CAAAAACATC AGAAGATATT 
AAAATAATAC AAGATTCAAA ATTTCGCAGA TTAAAAGCAA CTATAAATAT GCAACTTGCT 
TTACAAAAAT ATAACATATA TAGAACTGAT TGTTTTGATG AAGAGACTCG TTTGAAACGT 
TGTGCTGAAG ATTTAAGAAA AAAATTGCAG GAACTTAATA ATATAATCAT TAATGAAATA 
CAAAATCATT TAAATGCTGC AGTTGAAAAT ACAATTGCTG GTATAAGATA TTTTAGAGTG 
CAAGTTGATG GACCTAAACT TAAAGAAATA AGTGAAAGAA ATCCACTAGT TCCTAGATAT 
TTTACTGATT ATGGAACACC TAAAACATTA ACTGAAAGAG AAGCAACAAT GTATTCAGAT 
GCAGGATGTT ATCTTATGGC TCATAATGGA TGGGTAATGA ATGGCGATCC TTTAAAAAAT 
TTTGCAGATT CTGATTCTAA TGTTTATATA AGAAGAGAAC TTATTGCTTG GGGTGATAGT 
GTAAAATTGA GATATGGAGA TAAACCAGAA GATTGTCCTT TTCTATGGCA GCATATTAGT 
ACATATGTTG AACAAACAGC TAAAATTTTT GATGGTATTC GATTAGACAA TTGTCATTCA 
ACTCCTATTC CAGTTGCAGA GTATATGCTT GATGTTGCTC GTCGTATCCG TCCAGATTTG 
TACGTTGTTG CAGAATTATT TACAAATTCA GATCAAAAAG ATAATATATT TGTTAATCGT 
CTTGGTATCA CTTCTTTAAT TCGAGAAGCT ATGTCTGCAT GGGATAGCCA CGAAGAAGGT 
CGATTGGTAT ATCGATATGG AGGTGAACCA GTCGGTGCAT TTTTGCAATC GCGTAAACGA 
CCTTTAGTAC CAAGCATAGC ACATGCCCTT TTTTTAGATT TAACTCATGA TAATCCTAGC 
ACTGTGGAAA AACGCAGCGT ATTCGATTTA CTTCCAAGTG CTGCTCTTGT ATCAATGGCA 
GCATGTGCTA GTGGTAGTAA TCGTGGATAT GATGAATTAG TTCCTCATCA TATACATGTT 
GTAGATGAAA CAAGACAATA TTCATTTTGG ACAGATGATG AATGTTTGAT AGATGGTATC 
AAATTTGTAG GTTTTAAAAC TGGTATAATT GCTGCAAAGA AAGCATTAAA TGATTTGCAT 
TATATTCTTG GACAACAAAA ATTTTCTCAA GTATTTGTTG ATCAAATGGA TAATGATATA 
GTAGCTGTAA CAAGACACTC TCCAACAACT CATGATAGTG TAATNTTAAT TGCTTTTACT 
GCTTTTAAAC ATCCTGATCC TAATGCTAAT GATTTGAGAA GACATGTGAA ACCATTACGA 
GTGGAAGGTG TAATGGAAGA AATTATTTTG GAAGCATCCT TATGTCATGT AGAAGCAAAA 
AATGATATCT CACCTTTCCA TTATTCTGAA AAATATGTAC GAGATGAAAG TTATATAAAT 
GGTCTCTCAG AATATATTGT AAATCTTAAA GAACATATAC AAATTTGTGA TTCAGAAATT 
TTGGAAAAAG TTGATTCTGG TGATCCTAAA ATAACTCAAC TTAATTTTGT AAATTTTCAA 
CCAGGTTCTG TTATTGCTAT TCGTGTATCT CTTCATGCTA ATATAAAACC AGCTCTTGCA 
AAACTTCATA ATACTATTTC AATAATCACA TCCCAAAAAA GTTCAGACAT ACGCGTTATT 
GCTTCACGTA TGGATTTGAT AGATTTAAAT AAAGCTTTAT ATCGATGTGA TCAGGAAGAA 
CGCGATGAAA CTTCAAATAG ATTTGATGTA TATAATATTC CAGGTTATGG ACCACTTGTT 
TATGCAGGAT TGCAAGGTAT TATTTCTTTA TTAGCGGATA TTCGTCCTAA TAATGATTTA 
GGTCATCCAA TGTGTATTAA TCTCAGACAA GGTAATTGGT TAATAGATTA TACTTGGCAA 
CGGTTAAAAG AAGACGATGG AACAAAAGCA CTTGGAGAAT GGCTTGAAGA AGCTACTGAA 
CCATTTAAAT TAATACCTCG TTATTTAGTT CCTAGTTATT TTGATGTTAT AGTTGTAAAC 
GTTTATATGA TACTTCTCGA ACAATGCTAT AATCTAATGT CAAGCTTTGT GAAAGATGGT 
TCTACTTTTG TCAAGATCTT ATCTTTGGTT TCAATACAAA TGGGAGGAGT AATAAAATCA 
GCTCCATTAC CAGATTTGTC ACCAAATATA GATTCTCCTA AACCAAAGAT TAAACAATAT 
AATGACGAAA GTGAACAAAT ATGTATGACA TTATCTGCTG GTTTACCGCA TTTCACAACA 
GGATATATGA GAAATTGGGG AAGAGATACA TTTATTGCTT TAAGAGGACT ATTGATATTA 
ACTGGCAGAC ATACAGAAGC ACGATTTATT ATTCTTGGTT ATGCTGGAAC TTTACGACAT 
GGTCTTATAC CAAATTTACT TGATAAAGGA AAGAATGCTA GATATAACTG TCGAGATGCT 
TTATGGTGGT GGTTGTATAC AATTCAATGT TATATACAAG AAGTGCCAAA TGGTTTAAAA 
ATTTTAACAG ATAAAGTTTC AAGACTGTTT CCAACTGATG ATTCTCCTCC ATTACCCGCG 
GGACAAGTAG ATCAATCATT ACATGATGTT ATTCAAGAAG CTCTTTTAGT ACATTTTCAA 
GGACTTTGTT TTAGAGAAAG AAATGCTGGA AAACAGATTG ATGAACATAT GACAGATCGT 
GGTTTTAATA ATCAAATTGG AGTACATCCA GAAACAGGAT TTATATTTGG TGGAAATGAT 
GCAAATTGTG GTACGTGGAT GGATAAAATG GGATCTTCTG AAAAAGCTGG TAACAAAGGA 
AAACCAGCTA CTCCAAGGGA TGGTTCAGCT GTAGAAATAG TGGGTCTCAG TAAAAGTATT 
CTTAGTTTTT TAGCTGAATT ATATAAACAA AATCTTTTTC CTTATGGTAG TGTGCAAAGA 
AAAAATCGTG ATGGTTCTGT TGTAACATGG AGTTATAAAC AATGGGCAGA TAAAATTTCA 
TTAAATTTTG AAAAATATTT TTATATTAAT GAAATAGCAA AAGAAGGAGA ATTAAATTTA 
GAACTTATTC ATCGGCGTGG AATTTTTAAA GATAGTCATG GAGCAACACA AGCATGGGCT 
GATTATCAAC TACGACCTAA TTTTCCAGTT GCAATGGTTG TTGCTCCAGA GTTATTTAAT 
CCAGAACATG CATGGACAGC ATTAAAAAAA GTAGAAGAAA TTTTATTAGG GCCACTTGGA 
ATGAAAACAT TAGATCCTGC AGATTGGGCA TATAATGGAT ATTATGATAA TTCTAATGAT 
AGTGGAGACA CTAAATTAGC TCATGGTTGG AATTATCATC AAGGACCCGA ATGGATTTGG 
CCTATAGGTT ATTTTCTTCG AGCTCGATTG TATTTTGCTC CATTAATTGG TGGAAAAGAA 
GAATTATGCC GTACAATTGA ATCAACTGAA ACTATTATTT CTCGACATTT TATAGAAGCA 
TCTACAAATC ATTGGAGGGG TCTTCCAGAA CTTACTAACA AAGATGGGGA ATATTGCAAA 
GATAGTTGTA GATCGCAAGC TTGGAGTGCT GCTTCTATCC TAGAAGTTCT TTATGATTTA 
CATAAAATCA AACGGGAATT GCATTCTGAG GAGAATGAAA GAACGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC411488 ( XM_016915600.2 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_016771089.2 Apis mellifera glycogen debranching enzyme (LOC411488), mRNA.

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CloneID	OHd26024
Clone ID Related Accession (Same CDS sequence)	XM_016915600.2
Accession Version	XM_016915600.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1537200000000
Organism	Apis mellifera(honey bee)
Product	LOW QUALITY PROTEIN: glycogen debranching enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037649.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 19, 2018 this sequence version replaced XM_016915600.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

ATGGGTTCCA TACTTACAAT AAGCTATTTC TTTTCTGATG CAACAAGGAA ATTGATAAGG 
AAACTTAGGA CTATTTTCCA ATGCCTTTAC AAATATCATT ATTTAAAGTG CCTTTATTAT 
TATGCAGTGG TTAAAAATAT GTCAGAACAC ATGTCATCAA CTGAAAGTCA CGAATTTGAA 
CAAATAAGAG TTTTAACTCT AAATAATGGA GAACATCTTG ATGGTATATT ATATAGATTA 
AAGAAAGGAT GGAGAGTAGA ATTTCGTTTA GGCGCTTCTC TTCTTGGAAG AACTGTTGAT 
CTTTTTATAA ATTATCCTTT AGGAGAGAAT AAAAAATTTG AAAGACATAC ATATTATCTC 
TTAGAATGGA TTAATGAAAC AGCTAATATA TGTTTATCTC TAGCTGGTTC ATTTCATTAT 
TATCTTATAG ATCCTAATTC TGAAGGTATG AAGCCTATTG CTTCTGGATA TCTCCTTGTA 
GATCCAGAAC TTAAAATAGG GGAACATGGG AATGATTTAC CATTAGATTG TATTCAATGT 
CAAACTGTTC TTGCAAAATG TTTAGGATCA TTTTCTACCT GGGAAGATAA ATTACTAGTC 
GCTAAAAATT CTGGATATAA CATGATTCAT TTTACACCAA TTCAGGAATT GGGATATTCT 
AAATCTTCTT ATAGTCTTAG TGATCAATTA AAATTAAATC CTTCTTTTAA TGATGATAAT 
AAATCAATTA CATATGATGA TATTGAAAAA TTAATTAATA AAATGCGCAA TGAATGGAAT 
ATGTTAAGCA TCTGTGATAT TGTTTTGAAT CATACAGCAA ATGAAAGTCC TTTTTTGGTA 
TCTCATCCAG AATGTACATA TAATTGTTTG AATAGTCCTC ATTTACGTCC TGCATATATT 
TTGGATGCTG CATTATTTGA ATTAACAGTG CAAGTGGCTG CTGGAGAATG GGAATTTAAA 
GGCATCCCTT TGATAGTTGA AACTGAAGAA CATTTAAATT CAATTCGTCA TGCACTTCAC 
ACATATTTTT TGCCGCTTAT AAAAATACAC GAATTATATA TAATAGATAT TAATGAAACT 
ATTGCTGAGT TTTTGAATTT AGCACGAAAT CAAATGCCTC AAGACATCAT TGAATCATCA 
AAAACATCAG AAGATATTAA AATAATACAA GATTCAAAAT TTCGCAGATT AAAAGCAACT 
ATAAATATGC AACTTGCTTT ACAAAAATAT AACATATATA GAACTGATTG TTTTGATGAA 
GAGACTCGTT TGAAACGTTG TGCTGAAGAT TTAAGAAAAA AATTGCAGGA ACTTAATAAT 
ATAATCATTA ATGAAATACA AAATCATTTA AATGCTGCAG TTGAAAATAC AATTGCTGGT 
ATAAGATATT TTAGAGTGCA AGTTGATGGA CCTAAACTTA AAGAAATAAG TGAAAGAAAT 
CCACTAGTTC CTAGATATTT TACTGATTAT GGAACACCTA AAACATTAAC TGAAAGAGAA 
GCAACAATGT ATTCAGATGC AGGATGTTAT CTTATGGCTC ATAATGGATG GGTAATGAAT 
GGCGATCCTT TAAAAAATTT TGCAGATTCT GATTCTAATG TTTATATAAG AAGAGAACTT 
ATTGCTTGGG GTGATAGTGT AAAATTGAGA TATGGAGATA AACCAGAAGA TTGTCCTTTT 
CTATGGCAGC ATATTAGTAC ATATGTTGAA CAAACAGCTA AAATTTTTGA TGGTATTCGA 
TTAGACAATT GTCATTCAAC TCCTATTCCA GTTGCAGAGT ATATGCTTGA TGTTGCTCGT 
CGTATCCGTC CAGATTTGTA CGTTGTTGCA GAATTATTTA CAAATTCAGA TCAAAAAGAT 
AATATATTTG TTAATCGTCT TGGTATCACT TCTTTAATTC GAGAAGCTAT GTCTGCATGG 
GATAGCCACG AAGAAGGTCG ATTGGTATAT CGATATGGAG GTGAACCAGT CGGTGCATTT 
TTGCAATCGC GTAAACGACC TTTAGTACCA AGCATAGCAC ATGCCCTTTT TTTAGATTTA 
ACTCATGATA ATCCTAGCAC TGTGGAAAAA CGCAGCGTAT TCGATTTACT TCCAAGTGCT 
GCTCTTGTAT CAATGGCAGC ATGTGCTAGT GGTAGTAATC GTGGATATGA TGAATTAGTT 
CCTCATCATA TACATGTTGT AGATGAAACA AGACAATATT CATTTTGGAC AGATGATGAA 
TGTTTGATAG ATGGTATCAA ATTTGTAGGT TTTAAAACTG GTATAATTGC TGCAAAGAAA 
GCATTAAATG ATTTGCATTA TATTCTTGGA CAACAAAAAT TTTCTCAAGT ATTTGTTGAT 
CAAATGGATA ATGATATAGT AGCTGTAACA AGACACTCTC CAACAACTCA TGATAGTGTA 
ATTTTAATTG CTTTTACTGC TTTTAAACAT CCTGATCCTA ATGCTAATGA TTTGAGAAGA 
CATGTGAAAC CATTACGAGT GGAAGGTGTA ATGGAAGAAA TTATTTTGGA AGCATCCTTA 
TGTCATGTAG AAGCAAAAAA TGATATCTCA CCTTTCCATT ATTCTGAAAA ATATGTACGA 
GATGAAAGTT ATATAAATGG TCTCTCAGAA TATATTGTAA ATCTTAAAGA ACATATACAA 
ATTTGTGATT CAGAAATTTT GGAAAAAGTT GATTCTGGTG ATCCTAAAAT AACTCAACTT 
AATTTTGTAA ATTTTCAACC AGGTTCTGTT ATTGCTATTC GTGTATCTCT TCATGCTAAT 
ATAAAACCAG CTCTTGCAAA ACTTCATAAT ACTATTTCAA TAATCACATC CCAAAAAAGT 
TCAGACATAC GCGTTATTGC TTCACGTATG GATTTGATAG ATTTAAATAA AGCTTTATAT 
CGATGTGATC AGGAAGAACG CGATGAAACT TCAAATAGAT TTGATGTATA TAATATTCCA 
GGTTATGGAC CACTTGTTTA TGCAGGATTG CAAGGTATTA TTTCTTTATT AGCGGATATT 
CGTCCTAATA ATGATTTAGG TCATCCAATG TGTATTAATC TCAGACAAGG TAATTGGTTA 
ATAGATTATA CTTGGCAACG GTTAAAAGAA GACGATGGAA CAAAAGCACT TGGAGAATGG 
CTTGAAGAAG CTACTGAACC ATTTAAATTA ATACCTCGTT ATTTAGTTCC TAGTTATTTT 
GATGTTATAG TTGTAAACGT TTATATGATA CTTCTCGAAC AATGCTATAA TCTAATGTCA 
AGCTTTGTGA AAGATGGTTC TACTTTTGTC AAGATCTTAT CTTTGGTTTC AATACAAATG 
GGAGGAGTAA TAAAATCAGC TCCATTACCA GATTTGTCAC CAAATATAGA TTCTCCTAAA 
CCAAAGATTA AACAATATAA TGACGAAAGT GAACAAATAT GTATGACATT ATCTGCTGGT 
TTACCGCATT TCACAACAGG ATATATGAGA AATTGGGGAA GAGATACATT TATTGCTTTA 
AGAGGACTAT TGATATTAAC TGGCAGACAT ACAGAAGCAC GATTTATTAT TCTTGGTTAT 
GCTGGAACTT TACGACATGG TCTTATACCA AATTTACTTG ATAAAGGAAA GAATGCTAGA 
TATAACTGTC GAGATGCTTT ATGGTGGTGG TTGTATACAA TTCAATGTTA TATACAAGAA 
GTGCCAAATG GTTTAAAAAT TTTAACAGAT AAAGTTTCAA GACTGTTTCC AACTGATGAT 
TCTCCTCCAT TACCCGCGGG ACAAGTAGAT CAATCATTAC ATGATGTTAT TCAAGAAGCT 
CTTTTAGTAC ATTTTCAAGG ACTTTGTTTT AGAGAAAGAA ATGCTGGAAA ACAGATTGAT 
GAACATATGA CAGATCGTGG TTTTAATAAT CAAATTGGAG TACATCCAGA AACAGGATTT 
ATATTTGGTG GAAATGATGC AAATTGTGGT ACGTGGATGG ATAAAATGGG ATCTTCTGAA 
AAAGCTGGTA ACAAAGGAAA ACCAGCTACT CCAAGGGATG GTTCAGCTGT AGAAATAGTG 
GGTCTCAGTA AAAGTATTCT TAGTTTTTTA GCTGAATTAT ATAAACAAAA TCTTTTTCCT 
TATGGTAGTG TGCAAAGAAA AAATCGTGAT GGTTCTGTTG TAACATGGAG TTATAAACAA 
TGGGCAGATA AAATTTCATT AAATTTTGAA AAATATTTTT ATATTAATGA AATAGCAAAA 
GAAGGAGAAT TAAATTTAGA ACTTATTCAT CGGCGTGGAA TTTTTAAAGA TAGTCATGGA 
GCAACACAAG CATGGGCTGA TTATCAACTA CGACCTAATT TTCCAGTTGC AATGGTTGTT 
GCTCCAGAGT TATTTAATCC AGAACATGCA TGGACAGCAT TAAAAAAAGT AGAAGAAATT 
TTATTAGGGC CACTTGGAAT GAAAACATTA GATCCTGCAG ATTGGGCATA TAATGGATAT 
TATGATAATT CTAATGATAG TGGAGACACT AAATTAGCTC ATGGTTGGAA TTATCATCAA 
GGACCCGAAT GGATTTGGCC TATAGGTTAT TTTCTTCGAG CTCGATTGTA TTTTGCTCCA 
TTAATTGGTG GAAAAGAAGA ATTATGCCGT ACAATTGAAT CAACTGAAAC TATTATTTCT 
CGACATTTTA TAGAAGCATC TACAAATCAT TGGAGGGGTC TTCCAGAACT TACTAACAAA 
GATGGGGAAT ATTGCAAAGA TAGTTGTAGA TCGCAAGCTT GGAGTGCTGC TTCTATCCTA 
GAAGTTCTTT ATGATTTACA TAAAATCAAA CGGGAATTGC ATTCTGAGGA GAATGAAAGA 
ACGAATTGAT ATAATTTTGC GTGTGTTCAC CACCGCCAAT TACACTTAGA ACATTTACGA 
TTAGAAAAAG AACGTAGTAT TAGAAAGAAA TGTCACGATA ACAAATGGCA TATATTTAAC 
GCAGCTATAG AAACACCGAG CACGGAAACA CACGCATTTG ATATTTCGCA CGTAGATAAA 
ACTAACATGC CACCGTGCAA ATTTATAAAG GCTGATATGA CACCTACAAG TGATGATGTT 
TTTTTAATTC AAGGACTTAA AGAAAACGAA AATCAAATAG ATATCAAACA TGATTATATA 
TCACCTAGTG TTATTAATAA TTTAGAAATA ATTGAAAGTA TATCAAATAC TACTGAGTTA 
TCAATGATAA CGGAAAATAA ATGTCATAAG TCAGAACAAT TCATATATGG AAATGAACCT 
TTAAAATATG AAAGTAAATC TTTAACCAAA CTTAAAGATA ATATAAATGA TCATACTTGT 
ACAATATTAA TGACACCAAA TACAGGGGAA AGTAATGCAA TGACAAAATA CTCATCAAAA 
ATAGATCACG TTTATGAAGA AGTCAATGCT CTTGAATGTG ATATTATAAG TTATTACGAA 
AAATATAGTC CTACGACAGC AAAAACATTA ATAAATACAC TTTTTCTTCC TCGATATAAA 
ATTTGTAAAC AATGTGCCAA TTTACAAGGC AGTCCAGTAT ATTCTGATCG TTTGATATAT 
GCAAAAAAGC CATTATATGC AAAAGAAGTA TATTTATTTC AACAGAATGA AACTTTATAT 
TTCAGATATT CTTCAGAAGA TAATAAATGC GACAATACAT TATTTTTACA ATACGATGAT 
ATTCAACATT GTTTATTAGT TTATAATTAT TTAAAAACTA ATGCTTCCAG TATATTTTAT 
TTTTACGGCC CATCACGAGA TGTATTTGAT TTGAAAACAA CATTAAAACC AAATATTTTT 
ATTATAAATA GTAATACCAG AGGAGTAGTA AATACAGAGT TATATCATCG TTGCGCAAGT 
AATTTTTATA CAAGAATATC AAATAATGGC ATTATACCTA AAGTGTTTCA TATGAATCAT 
AATAATATTT TAAGCTCTAT AAAACATATT GGATACGATA CTGATTTGCA AAACTGGATG 
AATTATCGTA ATGATATTTT AAAATTGCAT AATCTCAAAC AAGATGATGT AATTGATCAT 
CGTGCAAATA GATCCAGTTT ATTACTATTA GAACCTCTAT TATTAATGCC AGAATTAGGA 
AACTCTAGGT ATAGGCACGG AAAAAATCGT ATTTCACAAT CAGAGCACAT AAAAATAGTA 
AAGATAATAA TTGCCTATAC TCTTTCTTTT TTTATTTTAG CAACAATCAC TTTTTATATA 
GTTTATTTTA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016771089.2
CDS	164..6358
Translation	MGSILTISYFFSDATRKLIRKLRTIFQCLYKYHYLKCLYYYAVVKNMSEHMSSTESHEFEQIRVLTLNNGEHLDGILYRLKKGWRVEFRLGASLLGRTVDLFINYPLGENKKFERHTYYLLEWINETANICLSLAGSFHYYLIDPNSEGMKPIASGYLLVDPELKIGEHGNDLPLDCIQCQTVLAKCLGSFSTWEDKLLVAKNSGYNMIHFTPIQELGYSKSSYSLSDQLKLNPSFNDDNKSITYDDIEKLINKMRNEWNMLSICDIVLNHTANESPFLVSHPECTYNCLNSPHLRPAYILDAALFELTVQVAAGEWEFKGIPLIVETEEHLNSIRHALHTYFLPLIKIHELYIIDINETIAEFLNLARNQMPQDIIESSKTSEDIKIIQDSKFRRLKATINMQLALQKYNIYRTDCFDEETRLKRCAEDLRKKLQELNNIIINEIQNHLNAAVENTIAGIRYFRVQVDGPKLKEISERNPLVPRYFTDYGTPKTLTEREATMYSDAGCYLMAHNGWVMNGDPLKNFADSDSNVYIRRELIAWGDSVKLRYGDKPEDCPFLWQHISTYVEQTAKIFDGIRLDNCHSTPIPVAEYMLDVARRIRPDLYVVAELFTNSDQKDNIFVNRLGITSLIREAMSAWDSHEEGRLVYRYGGEPVGAFLQSRKRPLVPSIAHALFLDLTHDNPSTVEKRSVFDLLPSAALVSMAACASGSNRGYDELVPHHIHVVDETRQYSFWTDDECLIDGIKFVGFKTGIIAAKKALNDLHYILGQQKFSQVFVDQMDNDIVAVTRHSPTTHDSVILIAFTAFKHPDPNANDLRRHVKPLRVEGVMEEIILEASLCHVEAKNDISPFHYSEKYVRDESYINGLSEYIVNLKEHIQICDSEILEKVDSGDPKITQLNFVNFQPGSVIAIRVSLHANIKPALAKLHNTISIITSQKSSDIRVIASRMDLIDLNKALYRCDQEERDETSNRFDVYNIPGYGPLVYAGLQGIISLLADIRPNNDLGHPMCINLRQGNWLIDYTWQRLKEDDGTKALGEWLEEATEPFKLIPRYLVPSYFDVIVVNVYMILLEQCYNLMSSFVKDGSTFVKILSLVSIQMGGVIKSAPLPDLSPNIDSPKPKIKQYNDESEQICMTLSAGLPHFTTGYMRNWGRDTFIALRGLLILTGRHTEARFIILGYAGTLRHGLIPNLLDKGKNARYNCRDALWWWLYTIQCYIQEVPNGLKILTDKVSRLFPTDDSPPLPAGQVDQSLHDVIQEALLVHFQGLCFRERNAGKQIDEHMTDRGFNNQIGVHPETGFIFGGNDANCGTWMDKMGSSEKAGNKGKPATPRDGSAVEIVGLSKSILSFLAELYKQNLFPYGSVQRKNRDGSVVTWSYKQWADKISLNFEKYFYINEIAKEGELNLELIHRRGIFKDSHGATQAWADYQLRPNFPVAMVVAPELFNPEHAWTALKKVEEILLGPLGMKTLDPADWAYNGYYDNSNDSGDTKLAHGWNYHQGPEWIWPIGYFLRARLYFAPLIGGKEELCRTIESTETIISRHFIEASTNHWRGLPELTNKDGEYCKDSCRSQAWSAASILEVLYDLHKIKRELHSEENERTN*YNFACVHHRQLHLEHLRLEKERSIRKKCHDNKWHIFNAAIETPSTETHAFDISHVDKTNMPPCKFIKADMTPTSDDVFLIQGLKENENQIDIKHDYISPSVINNLEIIESISNTTELSMITENKCHKSEQFIYGNEPLKYESKSLTKLKDNINDHTCTILMTPNTGESNAMTKYSSKIDHVYEEVNALECDIISYYEKYSPTTAKTLINTLFLPRYKICKQCANLQGSPVYSDRLIYAKKPLYAKEVYLFQQNETLYFRYSSEDNKCDNTLFLQYDDIQHCLLVYNYLKTNASSIFYFYGPSRDVFDLKTTLKPNIFIINSNTRGVVNTELYHRCASNFYTRISNNGIIPKVFHMNHNNILSSIKHIGYDTDLQNWMNYRNDILKLHNLKQDDVIDHRANRSSLLLLEPLLLMPELGNSRYRHGKNRISQSEHIKIVKIIIAYTLSFFILATITFYIVYFT

Target ORF information:

RefSeq Version	XM_016915600.2
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera glycogen debranching enzyme (LOC411488), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016915600.2

ORF Insert Sequence:

ATGGGTTCCA TACTTACAAT AAGCTATTTC TTTTCTGATG CAACAAGGAA ATTGATAAGG 
AAACTTAGGA CTATTTTCCA ATGCCTTTAC AAATATCATT ATTTAAAGTG CCTTTATTAT 
TATGCAGTGG TTAAAAATAT GTCAGAACAC ATGTCATCAA CTGAAAGTCA CGAATTTGAA 
CAAATAAGAG TTTTAACTCT AAATAATGGA GAACATCTTG ATGGTATATT ATATAGATTA 
AAGAAAGGAT GGAGAGTAGA ATTTCGTTTA GGCGCTTCTC TTCTTGGAAG AACTGTTGAT 
CTTTTTATAA ATTATCCTTT AGGAGAGAAT AAAAAATTTG AAAGACATAC ATATTATCTC 
TTAGAATGGA TTAATGAAAC AGCTAATATA TGTTTATCTC TAGCTGGTTC ATTTCATTAT 
TATCTTATAG ATCCTAATTC TGAAGGTATG AAGCCTATTG CTTCTGGATA TCTCCTTGTA 
GATCCAGAAC TTAAAATAGG GGAACATGGG AATGATTTAC CATTAGATTG TATTCAATGT 
CAAACTGTTC TTGCAAAATG TTTAGGATCA TTTTCTACCT GGGAAGATAA ATTACTAGTC 
GCTAAAAATT CTGGATATAA CATGATTCAT TTTACACCAA TTCAGGAATT GGGATATTCT 
AAATCTTCTT ATAGTCTTAG TGATCAATTA AAATTAAATC CTTCTTTTAA TGATGATAAT 
AAATCAATTA CATATGATGA TATTGAAAAA TTAATTAATA AAATGCGCAA TGAATGGAAT 
ATGTTAAGCA TCTGTGATAT TGTTTTGAAT CATACAGCAA ATGAAAGTCC TTTTTTGGTA 
TCTCATCCAG AATGTACATA TAATTGTTTG AATAGTCCTC ATTTACGTCC TGCATATATT 
TTGGATGCTG CATTATTTGA ATTAACAGTG CAAGTGGCTG CTGGAGAATG GGAATTTAAA 
GGCATCCCTT TGATAGTTGA AACTGAAGAA CATTTAAATT CAATTCGTCA TGCACTTCAC 
ACATATTTTT TGCCGCTTAT AAAAATACAC GAATTATATA TAATAGATAT TAATGAAACT 
ATTGCTGAGT TTTTGAATTT AGCACGAAAT CAAATGCCTC AAGACATCAT TGAATCATCA 
AAAACATCAG AAGATATTAA AATAATACAA GATTCAAAAT TTCGCAGATT AAAAGCAACT 
ATAAATATGC AACTTGCTTT ACAAAAATAT AACATATATA GAACTGATTG TTTTGATGAA 
GAGACTCGTT TGAAACGTTG TGCTGAAGAT TTAAGAAAAA AATTGCAGGA ACTTAATAAT 
ATAATCATTA ATGAAATACA AAATCATTTA AATGCTGCAG TTGAAAATAC AATTGCTGGT 
ATAAGATATT TTAGAGTGCA AGTTGATGGA CCTAAACTTA AAGAAATAAG TGAAAGAAAT 
CCACTAGTTC CTAGATATTT TACTGATTAT GGAACACCTA AAACATTAAC TGAAAGAGAA 
GCAACAATGT ATTCAGATGC AGGATGTTAT CTTATGGCTC ATAATGGATG GGTAATGAAT 
GGCGATCCTT TAAAAAATTT TGCAGATTCT GATTCTAATG TTTATATAAG AAGAGAACTT 
ATTGCTTGGG GTGATAGTGT AAAATTGAGA TATGGAGATA AACCAGAAGA TTGTCCTTTT 
CTATGGCAGC ATATTAGTAC ATATGTTGAA CAAACAGCTA AAATTTTTGA TGGTATTCGA 
TTAGACAATT GTCATTCAAC TCCTATTCCA GTTGCAGAGT ATATGCTTGA TGTTGCTCGT 
CGTATCCGTC CAGATTTGTA CGTTGTTGCA GAATTATTTA CAAATTCAGA TCAAAAAGAT 
AATATATTTG TTAATCGTCT TGGTATCACT TCTTTAATTC GAGAAGCTAT GTCTGCATGG 
GATAGCCACG AAGAAGGTCG ATTGGTATAT CGATATGGAG GTGAACCAGT CGGTGCATTT 
TTGCAATCGC GTAAACGACC TTTAGTACCA AGCATAGCAC ATGCCCTTTT TTTAGATTTA 
ACTCATGATA ATCCTAGCAC TGTGGAAAAA CGCAGCGTAT TCGATTTACT TCCAAGTGCT 
GCTCTTGTAT CAATGGCAGC ATGTGCTAGT GGTAGTAATC GTGGATATGA TGAATTAGTT 
CCTCATCATA TACATGTTGT AGATGAAACA AGACAATATT CATTTTGGAC AGATGATGAA 
TGTTTGATAG ATGGTATCAA ATTTGTAGGT TTTAAAACTG GTATAATTGC TGCAAAGAAA 
GCATTAAATG ATTTGCATTA TATTCTTGGA CAACAAAAAT TTTCTCAAGT ATTTGTTGAT 
CAAATGGATA ATGATATAGT AGCTGTAACA AGACACTCTC CAACAACTCA TGATAGTGTA 
ATTTTAATTG CTTTTACTGC TTTTAAACAT CCTGATCCTA ATGCTAATGA TTTGAGAAGA 
CATGTGAAAC CATTACGAGT GGAAGGTGTA ATGGAAGAAA TTATTTTGGA AGCATCCTTA 
TGTCATGTAG AAGCAAAAAA TGATATCTCA CCTTTCCATT ATTCTGAAAA ATATGTACGA 
GATGAAAGTT ATATAAATGG TCTCTCAGAA TATATTGTAA ATCTTAAAGA ACATATACAA 
ATTTGTGATT CAGAAATTTT GGAAAAAGTT GATTCTGGTG ATCCTAAAAT AACTCAACTT 
AATTTTGTAA ATTTTCAACC AGGTTCTGTT ATTGCTATTC GTGTATCTCT TCATGCTAAT 
ATAAAACCAG CTCTTGCAAA ACTTCATAAT ACTATTTCAA TAATCACATC CCAAAAAAGT 
TCAGACATAC GCGTTATTGC TTCACGTATG GATTTGATAG ATTTAAATAA AGCTTTATAT 
CGATGTGATC AGGAAGAACG CGATGAAACT TCAAATAGAT TTGATGTATA TAATATTCCA 
GGTTATGGAC CACTTGTTTA TGCAGGATTG CAAGGTATTA TTTCTTTATT AGCGGATATT 
CGTCCTAATA ATGATTTAGG TCATCCAATG TGTATTAATC TCAGACAAGG TAATTGGTTA 
ATAGATTATA CTTGGCAACG GTTAAAAGAA GACGATGGAA CAAAAGCACT TGGAGAATGG 
CTTGAAGAAG CTACTGAACC ATTTAAATTA ATACCTCGTT ATTTAGTTCC TAGTTATTTT 
GATGTTATAG TTGTAAACGT TTATATGATA CTTCTCGAAC AATGCTATAA TCTAATGTCA 
AGCTTTGTGA AAGATGGTTC TACTTTTGTC AAGATCTTAT CTTTGGTTTC AATACAAATG 
GGAGGAGTAA TAAAATCAGC TCCATTACCA GATTTGTCAC CAAATATAGA TTCTCCTAAA 
CCAAAGATTA AACAATATAA TGACGAAAGT GAACAAATAT GTATGACATT ATCTGCTGGT 
TTACCGCATT TCACAACAGG ATATATGAGA AATTGGGGAA GAGATACATT TATTGCTTTA 
AGAGGACTAT TGATATTAAC TGGCAGACAT ACAGAAGCAC GATTTATTAT TCTTGGTTAT 
GCTGGAACTT TACGACATGG TCTTATACCA AATTTACTTG ATAAAGGAAA GAATGCTAGA 
TATAACTGTC GAGATGCTTT ATGGTGGTGG TTGTATACAA TTCAATGTTA TATACAAGAA 
GTGCCAAATG GTTTAAAAAT TTTAACAGAT AAAGTTTCAA GACTGTTTCC AACTGATGAT 
TCTCCTCCAT TACCCGCGGG ACAAGTAGAT CAATCATTAC ATGATGTTAT TCAAGAAGCT 
CTTTTAGTAC ATTTTCAAGG ACTTTGTTTT AGAGAAAGAA ATGCTGGAAA ACAGATTGAT 
GAACATATGA CAGATCGTGG TTTTAATAAT CAAATTGGAG TACATCCAGA AACAGGATTT 
ATATTTGGTG GAAATGATGC AAATTGTGGT ACGTGGATGG ATAAAATGGG ATCTTCTGAA 
AAAGCTGGTA ACAAAGGAAA ACCAGCTACT CCAAGGGATG GTTCAGCTGT AGAAATAGTG 
GGTCTCAGTA AAAGTATTCT TAGTTTTTTA GCTGAATTAT ATAAACAAAA TCTTTTTCCT 
TATGGTAGTG TGCAAAGAAA AAATCGTGAT GGTTCTGTTG TAACATGGAG TTATAAACAA 
TGGGCAGATA AAATTTCATT AAATTTTGAA AAATATTTTT ATATTAATGA AATAGCAAAA 
GAAGGAGAAT TAAATTTAGA ACTTATTCAT CGGCGTGGAA TTTTTAAAGA TAGTCATGGA 
GCAACACAAG CATGGGCTGA TTATCAACTA CGACCTAATT TTCCAGTTGC AATGGTTGTT 
GCTCCAGAGT TATTTAATCC AGAACATGCA TGGACAGCAT TAAAAAAAGT AGAAGAAATT 
TTATTAGGGC CACTTGGAAT GAAAACATTA GATCCTGCAG ATTGGGCATA TAATGGATAT 
TATGATAATT CTAATGATAG TGGAGACACT AAATTAGCTC ATGGTTGGAA TTATCATCAA 
GGACCCGAAT GGATTTGGCC TATAGGTTAT TTTCTTCGAG CTCGATTGTA TTTTGCTCCA 
TTAATTGGTG GAAAAGAAGA ATTATGCCGT ACAATTGAAT CAACTGAAAC TATTATTTCT 
CGACATTTTA TAGAAGCATC TACAAATCAT TGGAGGGGTC TTCCAGAACT TACTAACAAA 
GATGGGGAAT ATTGCAAAGA TAGTTGTAGA TCGCAAGCTT GGAGTGCTGC TTCTATCCTA 
GAAGTTCTTT ATGATTTACA TAAAATCAAA CGGGAATTGC ATTCTGAGGA GAATGAAAGA 
ACGAATTGAT ATAATTTTGC GTGTGTTCAC CACCGCCAAT TACACTTAGA ACATTTACGA 
TTAGAAAAAG AACGTAGTAT TAGAAAGAAA TGTCACGATA ACAAATGGCA TATATTTAAC 
GCAGCTATAG AAACACCGAG CACGGAAACA CACGCATTTG ATATTTCGCA CGTAGATAAA 
ACTAACATGC CACCGTGCAA ATTTATAAAG GCTGATATGA CACCTACAAG TGATGATGTT 
TTTTTAATTC AAGGACTTAA AGAAAACGAA AATCAAATAG ATATCAAACA TGATTATATA 
TCACCTAGTG TTATTAATAA TTTAGAAATA ATTGAAAGTA TATCAAATAC TACTGAGTTA 
TCAATGATAA CGGAAAATAA ATGTCATAAG TCAGAACAAT TCATATATGG AAATGAACCT 
TTAAAATATG AAAGTAAATC TTTAACCAAA CTTAAAGATA ATATAAATGA TCATACTTGT 
ACAATATTAA TGACACCAAA TACAGGGGAA AGTAATGCAA TGACAAAATA CTCATCAAAA 
ATAGATCACG TTTATGAAGA AGTCAATGCT CTTGAATGTG ATATTATAAG TTATTACGAA 
AAATATAGTC CTACGACAGC AAAAACATTA ATAAATACAC TTTTTCTTCC TCGATATAAA 
ATTTGTAAAC AATGTGCCAA TTTACAAGGC AGTCCAGTAT ATTCTGATCG TTTGATATAT 
GCAAAAAAGC CATTATATGC AAAAGAAGTA TATTTATTTC AACAGAATGA AACTTTATAT 
TTCAGATATT CTTCAGAAGA TAATAAATGC GACAATACAT TATTTTTACA ATACGATGAT 
ATTCAACATT GTTTATTAGT TTATAATTAT TTAAAAACTA ATGCTTCCAG TATATTTTAT 
TTTTACGGCC CATCACGAGA TGTATTTGAT TTGAAAACAA CATTAAAACC AAATATTTTT 
ATTATAAATA GTAATACCAG AGGAGTAGTA AATACAGAGT TATATCATCG TTGCGCAAGT 
AATTTTTATA CAAGAATATC AAATAATGGC ATTATACCTA AAGTGTTTCA TATGAATCAT 
AATAATATTT TAAGCTCTAT AAAACATATT GGATACGATA CTGATTTGCA AAACTGGATG 
AATTATCGTA ATGATATTTT AAAATTGCAT AATCTCAAAC AAGATGATGT AATTGATCAT 
CGTGCAAATA GATCCAGTTT ATTACTATTA GAACCTCTAT TATTAATGCC AGAATTAGGA 
AACTCTAGGT ATAGGCACGG AAAAAATCGT ATTTCACAAT CAGAGCACAT AAAAATAGTA 
AAGATAATAA TTGCCTATAC TCTTTCTTTT TTTATTTTAG CAACAATCAC TTTTTATATA 
GTTTATTTTA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC411488 cDNA ORF clone, Apis mellifera(honey bee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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