LOC110495925 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC110495925
Entrez Gene ID	110495925
Full Name	von Willebrand factor D and EGF domain-containing protein-like
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021571449.1	XP_021427124.1	von Willebrand factor D and EGF domain-containing protein-like

Pathways from BioSystems
Gene Ontology

The following LOC110495925 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC110495925 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf19826	XM_021571449.1 Latest version!	Oncorhynchus mykiss von Willebrand factor D and EGF domain-containing protein-like (LOC110495925), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC110495925 ( XM_021571449.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021427124.1 Oncorhynchus mykiss von Willebrand factor D and EGF domain-containing protein-like (LOC110495925), mRNA.

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CloneID	OOf19826
Clone ID Related Accession (Same CDS sequence)	XM_021571449.1
Accession Version	XM_021571449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	von Willebrand factor D and EGF domain-containing protein-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035094.1) and transcript sequences (GBTD01043427.1 and GDKP01001268.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 556 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGATTGCG TAGTTTGCAG CCTCTCACAG GCGTCAGTCC CGGGATATCT GCGCATTGTG 
TTTCTCTGTT TGATGCAGTT GAGAGCGCTT GCCCAGTTAG CTCCAGACTG CTACCCGGGA 
GGTTACCGCA TGTTGAGAAA CCCGTATCGC AGCGTGGACT TTGACTCCAG TGACCTGCAG 
AACACTGCTA TACAGGACCT GATCTGCGAT CACTCCCTCT CCCCAGGATG GTACCGCTTC 
AGCATTAACA ACAAGCCTGC AGAGATGCCC ACCAGCTGTG TGGAAATGAA CAGATGTGGC 
ACCCAGGCGC CTGTGTGGCT GTCTCTCACT GATAGCTCAT TGCCCCGCCC AGGGGAGGTC 
CGCCAGCTCT CGGCCTGTGC CACCTGGCAG TTCTTCCACG GGAGCACCAA GGACTGCTGC 
CTCTTCCGCA TCCCCATCTC AGTACGCAAC TGTGGGGAGT TTCTCCTGTA CTACCTGCAG 
CCCACCCAGG GGTGTATGGG ATATTGTGCC AAAGTCATAT CAGAATTTGG ACCTAAATTA 
TGCCCCACGG GTGAGGTGGA AGTCAATGGG CAATGCAAAG CAAGTCTCCC TGCATTATCC 
TACAAGCCTG TGATCATCCC AGAGCTGGTG GGCAGCAGTG TCCACCTGCG ATGCACCTTC 
AGTGGGGTGA AGACCTCGGG ACTCCCCTTG GGCTATCTGG TGGTCTGGGC CCGACACCTG 
GCATCCAATA TGAAGGTGGA GATCAGGAAA GATTCTATCA TGGAGACCTT CTCATTGGTG 
GAGATGGACG GAGTCCACTT CAGACTGGGG GAGACGTTCT CCTGCAGTGT TTCCACGTTA 
CTGGGTAACT CCAGCCGGAC ACAATCTCTG GCCAAAGAGA GTGAGAGCTT CTACGCTGGA 
ATTAAGTTTG TTCCAGATGT GCTGCACATT GCGGAGGACG GAAAAGAGCA TGCTGTAACC 
ATTCGCAGTA CAGTGCCTAT CCCTTGTTAC GGGACAGAGC ATGGCCGACC ATGTGGAGTC 
ACTCTCACTC TGAGTGTCCG TGACTCAGAC AGCCTGGATC CCAGCGTGGA GGCCCCCAAC 
ATGGCCCTGT CTACCTGCCA GGTGGAGCTG CAGCCTACTG CAGCCTGCAG CCGGAGACAG 
GGGTGTGCCT GGGCCAGCCT CACCCTCACC GCCGTCACAG ACTTCACCCG CGACGGCAAC 
CGCCCCAGCC TACTCAGCGC CACGCCACGC TCTGACGCAC CGCGCCTCTG GAGGGGATAC 
AGCCCCACAC CTCTCAAGGT CACAGTTCAA GATGTTCCCA CTGCAAGCTG CTACTCTCTG 
ACTGATCCAC ACATCATTAC GTTGGATGGC AGACGCTATG AGAACCAACA GACGGGTACC 
TTCCTCCTCT ACAAGAGCCT CCACCGAGCG TTCGAGGTCC AGGCTCGTCA GTGGGACTGT 
GGCAGCCGCC ATTACGCTGT GTCCTGCAAC TGTGGAGTGG CGGCCAGGGA GGGAAACGAG 
GTGGTCATGC TGGACATGTG TAATGGTCAG CTTCAGGAGA CCAGGCCCCA GCTCTCTCTG 
AAGAACCTGG GAGGATATAA CAGAGTCTCC AGTCAGTATC GGATCAAGAT CCATGAATCT 
CACCAGGGGA AGAAAATTAC ATTGATCTTT CCATCTGGGG CCTTTGTAAG GGCGGACATC 
AGTGATTGGG GGATGAGCCT GTCAATCAGA GCTCCCAGTG TGGACTACAG CAGTACAACG 
GGACTCTGTG GGACGTTCGA CCGGAACGGT CACAACGACT TCCATGGACC TGATGGGACC 
ACCTATGGGA GCACTGAACA AGAACGCTTT GTGGAGGAAT GGAGGCTAGC CGCGGGGGAG 
AGCTTATTTG ACACCATGCC CCCTGTAATG GAGGTGGAGG AACTACGGCC TTTCTGTCGG 
TGTCAGGAGG GCTACAGCAT GTCTCTGCAT TTCACCACGG GCCACACCGG CGATAGCCAC 
TACAACTTTA ACCCCTCGTC ACCCCAGCAC CAGGCCCAGT GCCAGTCTCA TGACAACGTG 
GATTACACCT CTGTCTTCCC CTGGGTGGAC ACTACCACTC AGTACATTAG GAGCTCTGAG 
CCAGAACAAA GTGACCACCA GGGAGTGTCC ATCCTGAACG GCCCAGCTCT GCCTATGGAG 
ATACACAAAC AGCCGAGTTC CCAGATGGTG GACTATGGTG GGTTTGGGGA CCTCGGTGAT 
AAGAGTGAGA GAGAGGTTGA CAGGGAACTC AGAAGAGACT TCCTGGTTGC CGTAGACGAC 
AACAGGCCCA AGACAAAGAG ACAGGCGCTG TACGAGTTCC AGCCCATCTT CACCTCTCAG 
TCCCTGAGCC AAGCGGACCT GGAGAGCTTA GCCTATTTCT TCCCTGAGGA CCACCACCTG 
TCTGCAGAGC GACCAGTGGC CCAGCCTGTG TGGCCCACCC CGAGTGGGCT TACGTCGGTC 
AAGGCCCTGG AGGTGTGCCA GCTAGCCCTG GGGAACTCCA CGGTGGGAAC AGTGTGTAGA 
GGGCTGCTGG GCCGGAGGCT GGATGAGGCA GTGGACCTGT GCATCCTGGA TCTGCAGCTC 
AAGGATGACC TGGCCTGGGA GGAGGCTCTG GTGCACTACC TGGAGAACGA GTGTGAGAGG 
AGGCTATTGG AGAACCGGAC CCAGAGGGTC CTGGAGCTGG GGGAGTCCAG GGCTGGGATG 
GAGGGGGTTG AGGGGATCAT TGGGACGGTA CTCACCGCAC TGCGCTGCCC CAACTTCTGC 
AACGGGAACG GGCAGTGCAC TGAGTGGGGT TGTCAATGCT ATCCTGGTCA CAGCTTCTAC 
GACTGCAGCC TGGCTATCAG TCAGCCCGTG GAGTTGACAG ACCTGGAGAA CAGTGGTCTG 
TGTGACATCA GAGCGTTCGA CTGTCGCAGC GTCCGGGTCT TTGGCCTCGG CTTCATCGAC 
TCTCCCAACC TGGGCTGCCA CCTCACACGA CTGATGTACC TGAATGGTGA GCGGGTTCCG 
CGAGAGAAGC AGAGGACCAG AGCTACCTTC CTGAGCTCCA AGGCTTTAGA CTGTTCTGTC 
CCCTCACTGA ACAGCATGAT GACTATCAAC TCTGTAGACT TTATGGTGGA TGACAAACCC 
TATGCTCGCT GGGAAGTCAA GGTGACCAAT GACGGCTCTC AGTACAGTGA GCCTAAGTTG 
CTAACAATCT ACGATGGTGT GTGCCAGGTG TGTGAACCAA CCCACTCCGG ACTCTGTAAG 
TTAAAGGAGA GGACATGCAA TATAGATGGA ATGTGTTTTG CTAAAGGAGA CTCCAATCCT 
GCCAGCCCCT GCCTCCTCTG TAATCCAAAC ACATCCAAAT TCACATGGTC CGTAAATGAA 
GTCAACCAGC CCCCCAACCT GCACCAGCCT CAGGGGGGCC TCAGCACCTT CGCGGGGGAG 
AACTTTGTGT TCCAGTTCAC GGCGGCGGAC CCCGAGGGCT CGGCGCTCCT CTTCCTCCTG 
GAGCAGGGCC CTCCCGACGC CAGCCTCTCC CCCGCCGGCC TCCTCATCTG GAAAGTCCAT 
CCAGTGGAGA CACAGGGGAA GTCTCGGCAG AGCTTCGAGT TCACGCTGTC CGACGAGTGC 
AATGCCCAGA GCAGCTACAC CGTCGAGGTG GCTGTGAAAC CTTGTGGGTG TTTGAACGGC 
GGGACGTGCG TGACCAACGT GGCCTTCCCG GCGGGCAGCG GAGAATACCT GTGTGCGTGT 
CCCCAGGGGA TAGGTGGGGT CCTGTGTCAG GAGGACTTTG ACGCGTGTCT ATCGGCTCCA 
TGCGGTGCCG GGGTGTGTGT TGATACCGTT GATGGCTTCC TCTGTGAATG CCCTGCAGGA 
CTGAGAGGCG CTACCTGTCA GGAAGATGTG AATGAGTGTG AGAGGACCCC CTGCTTGCCT 
GGAGTCCAGT GCTTCAACAG CTTCGGCTCC TACAGGTGTG GAGGCTGTCC CCACGGCATG 
CTGAGAGATG GGACCACCTG CACAACAGCT GTACGGTCAA TCCCCATTGT CCCCAGTATT 
GGACCCACCA CTGTCCCTAC CACCACCACC ACCTCTAAGT CCTCACCCAA CAACAGACCA 
GACCAACACA ATCCACCCAG GATGGACACT GGCCGACCTG GAATCCCACC GACCAATCAG 
CCTGGACTCC CACAAACCAA CATGCCTGGA GTTCCTGAGA CCAACACGGC ACCTGTCAAG 
GCCCCAGCTG TACATACACC GTCGGAGAAT GGACAAAAAA CTGGCTTAGG GCTATCATCC 
CATGCAGGAA TCAACAACAA TGTGACAGGT ACGTGTGCCA GCCGACCGTG CTTCCTGGGG 
GTCCAGTGCA TCGACCGACG TCCACCCTAC ACGGGCTATG TCTGCGGGCG CTGCCCACCG 
CCCCTCAATG GCAATGGACG CACCTGCACC AAAACCGCTC GAGGTAACTC TCACCTGACC 
CAGCAGCAGC TGACAATAGC CAAGAGCAGC AAACTCCACC ACCACAGCAA CAGTAAAGTG 
TCCCAACTCC ACCTTCCCAC CTTGCCCCCC AGGCACGCAG TCAAACACCT CTCCTCCCTC 
TCCTCGCCAG TCTCCAGAGG AGCCTATCAG AGCCCCCCGC ACCTCCCCCT CTCGCCCGCC 
CGAGGGCCAA TCACTGGGTG GAGAGAGGCG GCCACACCCC TATTTCCTAG ACACACCCCT 
ATGTCACCTG AAGGTTCCAA CAGCCTCACC ATGGGCAGGC ATCATGCTGT CAGTGCCCTC 
ACTAGGACTC GTGCCAAAAC AGGTACCAAT GCCAGCGCTA CCTCACTCAA GGTGACAACC 
AGAGAGTCTG TAGCTGTCGT GCGGAGTGCC ACCTCTTCCA AGGTTACTCC TCCATACTCC 
ACCTACCCCA AGGTTACTCC ACCCCAATCC AAGGTGACTC CACCCCAATC CAAGGTGACT 
CCACCCCATT CCTCAGCCAG CACAGAGTCC GGGCAGACAA CCCAAGGGAA GCTCATAAAT 
GTGGTAGTGG TGCCCACCAT GACCAAGCCC TGGACCCTCC CTAAAGCCGT GGCCCCGCTG 
ACCGCAGCCC TCTCCTCCAT TACCTACTCC CTCTCTGAGT CCGAGTTCTC CGCCGACGGA 
GCACTGACAG GGCCTGGCTC GAACCCCTCT GACCTACCTG ACCACCCACA GGAACCCCTG 
GTCTCCCTCG CCCTCACCAC CCCTGCCCGG AAGCACCACT ACACTCCCCC CCTCCACAGG 
GCCACGTCTC CCCACCTCCA CCGTGGGACT AGGATCAGTA ATACTGTGGC AGTGGAGCGG 
GTGCGGCCTC TGACCTGCGC TGACATGCCG TGTTTCCCAG GGGTCCCCTG TGAACCCACC 
AGGGACGGGG GCTTCCACTG TGGAAGGTGT CCCCTGGGAT ACACCGGAGA CAGACAAACA 
TGCAGAGCTG TGTGCAGGCA TGCGTGTGGC AGGAACATGG AGTGTGCCGC TCCCAACACA 
TGCCGCTGTA AACCAGGCTA CACCGGGACC AACTGTCAAA CAGCGATCTG TAAACCTGAG 
TGTAGGAACG GAGGCCAGTG CGTCGCCCCG GGGGCGTGTG AGTGCCTGAA AGGCAACCAC 
GGGGAGACGT GTGAGACAGC TCTGTGCAGT CTGCCCTGTG AGCATGGAGG AACATGCGTG 
GGACCCAGAA CCTGCTCCTG TCCCTACGGC TTTGTGGGCC CCAGATGTGA GACCATGGTG 
TGTAACCGCC ACTGTCACAA CGGAGGAGAG TGTGTGTCTC CTGATGAGTG TTCCTGTCTG 
CCTGGCTGGA CCGGACCCTC CTGTGAGACA GCTGTGTGCT TCCCTATGTG TCGGAATGGA 
GGGACTTGTC TTCGTCCGAA CACATGTGGC TGTCCCCTGG GCTTCTACGG CGCCCAGTGT 
CAAAACGCTG TCTGCAGCCC TGCCTGTAAG AACGGGGGCG TATGCATGAG AAACAGCGTC 
TGCTCCTGCG CCGAGGGATA CACCGGATGG CGCTGTGAAA AAAGTGTGTG TGAGCCCATG 
TGTATGAACG GCGGGCGGTG CGTGGGTCCA GATGTGTGTG ACTGTCCATC TGGATGGAGA 
GGAAAGCGGT GCGATAAACC CACTTGTCTA CAGAAGTGTC TGAATGGAGG AGAGTGTGTT 
GGCCATAACA CTTGCCACTG TCCTCCAGGC TGGCAGGGCA TGCTATGCCA AGCCCCACTC 
TGTGAACAGA AGTGTCTTTA TGGGAGCCGA TGTATTCGCC CTAATGTATG TGCCTGCAGG 
AAAGGGTATT CAGGGGTCCT GTGTTCCCAT AAGCTCCCAA TTCCACGAGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021427124.1
CDS	277..6570
Translation	MDCVVCSLSQASVPGYLRIVFLCLMQLRALAQLAPDCYPGGYRMLRNPYRSVDFDSSDLQNTAIQDLICDHSLSPGWYRFSINNKPAEMPTSCVEMNRCGTQAPVWLSLTDSSLPRPGEVRQLSACATWQFFHGSTKDCCLFRIPISVRNCGEFLLYYLQPTQGCMGYCAKVISEFGPKLCPTGEVEVNGQCKASLPALSYKPVIIPELVGSSVHLRCTFSGVKTSGLPLGYLVVWARHLASNMKVEIRKDSIMETFSLVEMDGVHFRLGETFSCSVSTLLGNSSRTQSLAKESESFYAGIKFVPDVLHIAEDGKEHAVTIRSTVPIPCYGTEHGRPCGVTLTLSVRDSDSLDPSVEAPNMALSTCQVELQPTAACSRRQGCAWASLTLTAVTDFTRDGNRPSLLSATPRSDAPRLWRGYSPTPLKVTVQDVPTASCYSLTDPHIITLDGRRYENQQTGTFLLYKSLHRAFEVQARQWDCGSRHYAVSCNCGVAAREGNEVVMLDMCNGQLQETRPQLSLKNLGGYNRVSSQYRIKIHESHQGKKITLIFPSGAFVRADISDWGMSLSIRAPSVDYSSTTGLCGTFDRNGHNDFHGPDGTTYGSTEQERFVEEWRLAAGESLFDTMPPVMEVEELRPFCRCQEGYSMSLHFTTGHTGDSHYNFNPSSPQHQAQCQSHDNVDYTSVFPWVDTTTQYIRSSEPEQSDHQGVSILNGPALPMEIHKQPSSQMVDYGGFGDLGDKSEREVDRELRRDFLVAVDDNRPKTKRQALYEFQPIFTSQSLSQADLESLAYFFPEDHHLSAERPVAQPVWPTPSGLTSVKALEVCQLALGNSTVGTVCRGLLGRRLDEAVDLCILDLQLKDDLAWEEALVHYLENECERRLLENRTQRVLELGESRAGMEGVEGIIGTVLTALRCPNFCNGNGQCTEWGCQCYPGHSFYDCSLAISQPVELTDLENSGLCDIRAFDCRSVRVFGLGFIDSPNLGCHLTRLMYLNGERVPREKQRTRATFLSSKALDCSVPSLNSMMTINSVDFMVDDKPYARWEVKVTNDGSQYSEPKLLTIYDGVCQVCEPTHSGLCKLKERTCNIDGMCFAKGDSNPASPCLLCNPNTSKFTWSVNEVNQPPNLHQPQGGLSTFAGENFVFQFTAADPEGSALLFLLEQGPPDASLSPAGLLIWKVHPVETQGKSRQSFEFTLSDECNAQSSYTVEVAVKPCGCLNGGTCVTNVAFPAGSGEYLCACPQGIGGVLCQEDFDACLSAPCGAGVCVDTVDGFLCECPAGLRGATCQEDVNECERTPCLPGVQCFNSFGSYRCGGCPHGMLRDGTTCTTAVRSIPIVPSIGPTTVPTTTTTSKSSPNNRPDQHNPPRMDTGRPGIPPTNQPGLPQTNMPGVPETNTAPVKAPAVHTPSENGQKTGLGLSSHAGINNNVTGTCASRPCFLGVQCIDRRPPYTGYVCGRCPPPLNGNGRTCTKTARGNSHLTQQQLTIAKSSKLHHHSNSKVSQLHLPTLPPRHAVKHLSSLSSPVSRGAYQSPPHLPLSPARGPITGWREAATPLFPRHTPMSPEGSNSLTMGRHHAVSALTRTRAKTGTNASATSLKVTTRESVAVVRSATSSKVTPPYSTYPKVTPPQSKVTPPQSKVTPPHSSASTESGQTTQGKLINVVVVPTMTKPWTLPKAVAPLTAALSSITYSLSESEFSADGALTGPGSNPSDLPDHPQEPLVSLALTTPARKHHYTPPLHRATSPHLHRGTRISNTVAVERVRPLTCADMPCFPGVPCEPTRDGGFHCGRCPLGYTGDRQTCRAVCRHACGRNMECAAPNTCRCKPGYTGTNCQTAICKPECRNGGQCVAPGACECLKGNHGETCETALCSLPCEHGGTCVGPRTCSCPYGFVGPRCETMVCNRHCHNGGECVSPDECSCLPGWTGPSCETAVCFPMCRNGGTCLRPNTCGCPLGFYGAQCQNAVCSPACKNGGVCMRNSVCSCAEGYTGWRCEKSVCEPMCMNGGRCVGPDVCDCPSGWRGKRCDKPTCLQKCLNGGECVGHNTCHCPPGWQGMLCQAPLCEQKCLYGSRCIRPNVCACRKGYSGVLCSHKLPIPRG

Target ORF information:

RefSeq Version	XM_021571449.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss von Willebrand factor D and EGF domain-containing protein-like (LOC110495925), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021571449.1

ORF Insert Sequence:

ATGGATTGCG TAGTTTGCAG CCTCTCACAG GCGTCAGTCC CGGGATATCT GCGCATTGTG 
TTTCTCTGTT TGATGCAGTT GAGAGCGCTT GCCCAGTTAG CTCCAGACTG CTACCCGGGA 
GGTTACCGCA TGTTGAGAAA CCCGTATCGC AGCGTGGACT TTGACTCCAG TGACCTGCAG 
AACACTGCTA TACAGGACCT GATCTGCGAT CACTCCCTCT CCCCAGGATG GTACCGCTTC 
AGCATTAACA ACAAGCCTGC AGAGATGCCC ACCAGCTGTG TGGAAATGAA CAGATGTGGC 
ACCCAGGCGC CTGTGTGGCT GTCTCTCACT GATAGCTCAT TGCCCCGCCC AGGGGAGGTC 
CGCCAGCTCT CGGCCTGTGC CACCTGGCAG TTCTTCCACG GGAGCACCAA GGACTGCTGC 
CTCTTCCGCA TCCCCATCTC AGTACGCAAC TGTGGGGAGT TTCTCCTGTA CTACCTGCAG 
CCCACCCAGG GGTGTATGGG ATATTGTGCC AAAGTCATAT CAGAATTTGG ACCTAAATTA 
TGCCCCACGG GTGAGGTGGA AGTCAATGGG CAATGCAAAG CAAGTCTCCC TGCATTATCC 
TACAAGCCTG TGATCATCCC AGAGCTGGTG GGCAGCAGTG TCCACCTGCG ATGCACCTTC 
AGTGGGGTGA AGACCTCGGG ACTCCCCTTG GGCTATCTGG TGGTCTGGGC CCGACACCTG 
GCATCCAATA TGAAGGTGGA GATCAGGAAA GATTCTATCA TGGAGACCTT CTCATTGGTG 
GAGATGGACG GAGTCCACTT CAGACTGGGG GAGACGTTCT CCTGCAGTGT TTCCACGTTA 
CTGGGTAACT CCAGCCGGAC ACAATCTCTG GCCAAAGAGA GTGAGAGCTT CTACGCTGGA 
ATTAAGTTTG TTCCAGATGT GCTGCACATT GCGGAGGACG GAAAAGAGCA TGCTGTAACC 
ATTCGCAGTA CAGTGCCTAT CCCTTGTTAC GGGACAGAGC ATGGCCGACC ATGTGGAGTC 
ACTCTCACTC TGAGTGTCCG TGACTCAGAC AGCCTGGATC CCAGCGTGGA GGCCCCCAAC 
ATGGCCCTGT CTACCTGCCA GGTGGAGCTG CAGCCTACTG CAGCCTGCAG CCGGAGACAG 
GGGTGTGCCT GGGCCAGCCT CACCCTCACC GCCGTCACAG ACTTCACCCG CGACGGCAAC 
CGCCCCAGCC TACTCAGCGC CACGCCACGC TCTGACGCAC CGCGCCTCTG GAGGGGATAC 
AGCCCCACAC CTCTCAAGGT CACAGTTCAA GATGTTCCCA CTGCAAGCTG CTACTCTCTG 
ACTGATCCAC ACATCATTAC GTTGGATGGC AGACGCTATG AGAACCAACA GACGGGTACC 
TTCCTCCTCT ACAAGAGCCT CCACCGAGCG TTCGAGGTCC AGGCTCGTCA GTGGGACTGT 
GGCAGCCGCC ATTACGCTGT GTCCTGCAAC TGTGGAGTGG CGGCCAGGGA GGGAAACGAG 
GTGGTCATGC TGGACATGTG TAATGGTCAG CTTCAGGAGA CCAGGCCCCA GCTCTCTCTG 
AAGAACCTGG GAGGATATAA CAGAGTCTCC AGTCAGTATC GGATCAAGAT CCATGAATCT 
CACCAGGGGA AGAAAATTAC ATTGATCTTT CCATCTGGGG CCTTTGTAAG GGCGGACATC 
AGTGATTGGG GGATGAGCCT GTCAATCAGA GCTCCCAGTG TGGACTACAG CAGTACAACG 
GGACTCTGTG GGACGTTCGA CCGGAACGGT CACAACGACT TCCATGGACC TGATGGGACC 
ACCTATGGGA GCACTGAACA AGAACGCTTT GTGGAGGAAT GGAGGCTAGC CGCGGGGGAG 
AGCTTATTTG ACACCATGCC CCCTGTAATG GAGGTGGAGG AACTACGGCC TTTCTGTCGG 
TGTCAGGAGG GCTACAGCAT GTCTCTGCAT TTCACCACGG GCCACACCGG CGATAGCCAC 
TACAACTTTA ACCCCTCGTC ACCCCAGCAC CAGGCCCAGT GCCAGTCTCA TGACAACGTG 
GATTACACCT CTGTCTTCCC CTGGGTGGAC ACTACCACTC AGTACATTAG GAGCTCTGAG 
CCAGAACAAA GTGACCACCA GGGAGTGTCC ATCCTGAACG GCCCAGCTCT GCCTATGGAG 
ATACACAAAC AGCCGAGTTC CCAGATGGTG GACTATGGTG GGTTTGGGGA CCTCGGTGAT 
AAGAGTGAGA GAGAGGTTGA CAGGGAACTC AGAAGAGACT TCCTGGTTGC CGTAGACGAC 
AACAGGCCCA AGACAAAGAG ACAGGCGCTG TACGAGTTCC AGCCCATCTT CACCTCTCAG 
TCCCTGAGCC AAGCGGACCT GGAGAGCTTA GCCTATTTCT TCCCTGAGGA CCACCACCTG 
TCTGCAGAGC GACCAGTGGC CCAGCCTGTG TGGCCCACCC CGAGTGGGCT TACGTCGGTC 
AAGGCCCTGG AGGTGTGCCA GCTAGCCCTG GGGAACTCCA CGGTGGGAAC AGTGTGTAGA 
GGGCTGCTGG GCCGGAGGCT GGATGAGGCA GTGGACCTGT GCATCCTGGA TCTGCAGCTC 
AAGGATGACC TGGCCTGGGA GGAGGCTCTG GTGCACTACC TGGAGAACGA GTGTGAGAGG 
AGGCTATTGG AGAACCGGAC CCAGAGGGTC CTGGAGCTGG GGGAGTCCAG GGCTGGGATG 
GAGGGGGTTG AGGGGATCAT TGGGACGGTA CTCACCGCAC TGCGCTGCCC CAACTTCTGC 
AACGGGAACG GGCAGTGCAC TGAGTGGGGT TGTCAATGCT ATCCTGGTCA CAGCTTCTAC 
GACTGCAGCC TGGCTATCAG TCAGCCCGTG GAGTTGACAG ACCTGGAGAA CAGTGGTCTG 
TGTGACATCA GAGCGTTCGA CTGTCGCAGC GTCCGGGTCT TTGGCCTCGG CTTCATCGAC 
TCTCCCAACC TGGGCTGCCA CCTCACACGA CTGATGTACC TGAATGGTGA GCGGGTTCCG 
CGAGAGAAGC AGAGGACCAG AGCTACCTTC CTGAGCTCCA AGGCTTTAGA CTGTTCTGTC 
CCCTCACTGA ACAGCATGAT GACTATCAAC TCTGTAGACT TTATGGTGGA TGACAAACCC 
TATGCTCGCT GGGAAGTCAA GGTGACCAAT GACGGCTCTC AGTACAGTGA GCCTAAGTTG 
CTAACAATCT ACGATGGTGT GTGCCAGGTG TGTGAACCAA CCCACTCCGG ACTCTGTAAG 
TTAAAGGAGA GGACATGCAA TATAGATGGA ATGTGTTTTG CTAAAGGAGA CTCCAATCCT 
GCCAGCCCCT GCCTCCTCTG TAATCCAAAC ACATCCAAAT TCACATGGTC CGTAAATGAA 
GTCAACCAGC CCCCCAACCT GCACCAGCCT CAGGGGGGCC TCAGCACCTT CGCGGGGGAG 
AACTTTGTGT TCCAGTTCAC GGCGGCGGAC CCCGAGGGCT CGGCGCTCCT CTTCCTCCTG 
GAGCAGGGCC CTCCCGACGC CAGCCTCTCC CCCGCCGGCC TCCTCATCTG GAAAGTCCAT 
CCAGTGGAGA CACAGGGGAA GTCTCGGCAG AGCTTCGAGT TCACGCTGTC CGACGAGTGC 
AATGCCCAGA GCAGCTACAC CGTCGAGGTG GCTGTGAAAC CTTGTGGGTG TTTGAACGGC 
GGGACGTGCG TGACCAACGT GGCCTTCCCG GCGGGCAGCG GAGAATACCT GTGTGCGTGT 
CCCCAGGGGA TAGGTGGGGT CCTGTGTCAG GAGGACTTTG ACGCGTGTCT ATCGGCTCCA 
TGCGGTGCCG GGGTGTGTGT TGATACCGTT GATGGCTTCC TCTGTGAATG CCCTGCAGGA 
CTGAGAGGCG CTACCTGTCA GGAAGATGTG AATGAGTGTG AGAGGACCCC CTGCTTGCCT 
GGAGTCCAGT GCTTCAACAG CTTCGGCTCC TACAGGTGTG GAGGCTGTCC CCACGGCATG 
CTGAGAGATG GGACCACCTG CACAACAGCT GTACGGTCAA TCCCCATTGT CCCCAGTATT 
GGACCCACCA CTGTCCCTAC CACCACCACC ACCTCTAAGT CCTCACCCAA CAACAGACCA 
GACCAACACA ATCCACCCAG GATGGACACT GGCCGACCTG GAATCCCACC GACCAATCAG 
CCTGGACTCC CACAAACCAA CATGCCTGGA GTTCCTGAGA CCAACACGGC ACCTGTCAAG 
GCCCCAGCTG TACATACACC GTCGGAGAAT GGACAAAAAA CTGGCTTAGG GCTATCATCC 
CATGCAGGAA TCAACAACAA TGTGACAGGT ACGTGTGCCA GCCGACCGTG CTTCCTGGGG 
GTCCAGTGCA TCGACCGACG TCCACCCTAC ACGGGCTATG TCTGCGGGCG CTGCCCACCG 
CCCCTCAATG GCAATGGACG CACCTGCACC AAAACCGCTC GAGGTAACTC TCACCTGACC 
CAGCAGCAGC TGACAATAGC CAAGAGCAGC AAACTCCACC ACCACAGCAA CAGTAAAGTG 
TCCCAACTCC ACCTTCCCAC CTTGCCCCCC AGGCACGCAG TCAAACACCT CTCCTCCCTC 
TCCTCGCCAG TCTCCAGAGG AGCCTATCAG AGCCCCCCGC ACCTCCCCCT CTCGCCCGCC 
CGAGGGCCAA TCACTGGGTG GAGAGAGGCG GCCACACCCC TATTTCCTAG ACACACCCCT 
ATGTCACCTG AAGGTTCCAA CAGCCTCACC ATGGGCAGGC ATCATGCTGT CAGTGCCCTC 
ACTAGGACTC GTGCCAAAAC AGGTACCAAT GCCAGCGCTA CCTCACTCAA GGTGACAACC 
AGAGAGTCTG TAGCTGTCGT GCGGAGTGCC ACCTCTTCCA AGGTTACTCC TCCATACTCC 
ACCTACCCCA AGGTTACTCC ACCCCAATCC AAGGTGACTC CACCCCAATC CAAGGTGACT 
CCACCCCATT CCTCAGCCAG CACAGAGTCC GGGCAGACAA CCCAAGGGAA GCTCATAAAT 
GTGGTAGTGG TGCCCACCAT GACCAAGCCC TGGACCCTCC CTAAAGCCGT GGCCCCGCTG 
ACCGCAGCCC TCTCCTCCAT TACCTACTCC CTCTCTGAGT CCGAGTTCTC CGCCGACGGA 
GCACTGACAG GGCCTGGCTC GAACCCCTCT GACCTACCTG ACCACCCACA GGAACCCCTG 
GTCTCCCTCG CCCTCACCAC CCCTGCCCGG AAGCACCACT ACACTCCCCC CCTCCACAGG 
GCCACGTCTC CCCACCTCCA CCGTGGGACT AGGATCAGTA ATACTGTGGC AGTGGAGCGG 
GTGCGGCCTC TGACCTGCGC TGACATGCCG TGTTTCCCAG GGGTCCCCTG TGAACCCACC 
AGGGACGGGG GCTTCCACTG TGGAAGGTGT CCCCTGGGAT ACACCGGAGA CAGACAAACA 
TGCAGAGCTG TGTGCAGGCA TGCGTGTGGC AGGAACATGG AGTGTGCCGC TCCCAACACA 
TGCCGCTGTA AACCAGGCTA CACCGGGACC AACTGTCAAA CAGCGATCTG TAAACCTGAG 
TGTAGGAACG GAGGCCAGTG CGTCGCCCCG GGGGCGTGTG AGTGCCTGAA AGGCAACCAC 
GGGGAGACGT GTGAGACAGC TCTGTGCAGT CTGCCCTGTG AGCATGGAGG AACATGCGTG 
GGACCCAGAA CCTGCTCCTG TCCCTACGGC TTTGTGGGCC CCAGATGTGA GACCATGGTG 
TGTAACCGCC ACTGTCACAA CGGAGGAGAG TGTGTGTCTC CTGATGAGTG TTCCTGTCTG 
CCTGGCTGGA CCGGACCCTC CTGTGAGACA GCTGTGTGCT TCCCTATGTG TCGGAATGGA 
GGGACTTGTC TTCGTCCGAA CACATGTGGC TGTCCCCTGG GCTTCTACGG CGCCCAGTGT 
CAAAACGCTG TCTGCAGCCC TGCCTGTAAG AACGGGGGCG TATGCATGAG AAACAGCGTC 
TGCTCCTGCG CCGAGGGATA CACCGGATGG CGCTGTGAAA AAAGTGTGTG TGAGCCCATG 
TGTATGAACG GCGGGCGGTG CGTGGGTCCA GATGTGTGTG ACTGTCCATC TGGATGGAGA 
GGAAAGCGGT GCGATAAACC CACTTGTCTA CAGAAGTGTC TGAATGGAGG AGAGTGTGTT 
GGCCATAACA CTTGCCACTG TCCTCCAGGC TGGCAGGGCA TGCTATGCCA AGCCCCACTC 
TGTGAACAGA AGTGTCTTTA TGGGAGCCGA TGTATTCGCC CTAATGTATG TGCCTGCAGG 
AAAGGGTATT CAGGGGTCCT GTGTTCCCAT AAGCTCCCAA TTCCACGAGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC110495925 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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