SPEF2 gene cDNA ORF clone, Chlorocebus sabaeus(green monkey)

mRNA	Protein	Name
XM_007961357.1	XP_007959548.1	sperm flagellar protein 2 isoform X11
XM_007961358.1	XP_007959549.1	sperm flagellar protein 2 isoform X12
XM_007961360.1	XP_007959551.1	sperm flagellar protein 2 isoform X14
XM_007961347.1	XP_007959538.1	sperm flagellar protein 2 isoform X2
XM_007961362.1	XP_007959553.1	sperm flagellar protein 2 isoform X16
XM_007961353.1	XP_007959544.1	sperm flagellar protein 2 isoform X7
XM_007961354.1	XP_007959545.1	sperm flagellar protein 2 isoform X8
XM_007961359.1	XP_007959550.1	sperm flagellar protein 2 isoform X13
XM_007961348.1	XP_007959539.1	sperm flagellar protein 2 isoform X3
XM_007961352.1	XP_007959543.1	sperm flagellar protein 2 isoform X6
XM_007961346.1	XP_007959537.1	sperm flagellar protein 2 isoform X1
XM_007961350.1	XP_007959541.1	sperm flagellar protein 2 isoform X5
XM_007961355.1	XP_007959546.1	sperm flagellar protein 2 isoform X9
XM_007961356.1	XP_007959547.1	sperm flagellar protein 2 isoform X10
XM_007961361.1	XP_007959552.1	sperm flagellar protein 2 isoform X15
XM_007961349.1	XP_007959540.1	sperm flagellar protein 2 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCh83470	XM_007961360.1 Latest version!	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCh83471	XM_007961361.1 Latest version!	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCh83463	XM_007961353.1 Latest version!	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCh83464	XM_007961354.1 Latest version!	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCh83465	XM_007961355.1 Latest version!	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCh83466	XM_007961356.1 Latest version!	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCh83467	XM_007961357.1 Latest version!	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCh83468	XM_007961358.1 Latest version!	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCh83469	XM_007961359.1 Latest version!	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCh83472	XM_007961362.1 Latest version!	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCh83457	XM_007961346.1 Latest version!	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCh83458	XM_007961347.1 Latest version!	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCh83459	XM_007961348.1 Latest version!	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCh83460	XM_007961349.1 Latest version!	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCh83461	XM_007961350.1 Latest version!	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCh83462	XM_007961352.1 Latest version!	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

SPEF2 ( XM_007961360.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007959551.1 Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OCh83470
Clone ID Related Accession (Same CDS sequence)	XM_007961360.1
Accession Version	XM_007961360.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	sperm flagellar protein 2 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735239.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG CAAGTGATCC ACAAAATGTA 
TTTTCAGCTG GTCCAGTTTC AAATGAAGGA TTACCAGAAA CAGAAGGTGA AACGATACTA 
AGTGCTAATG CTGATAAAAC ACCAAAAGCT GAAGAAATCA AATCCAGTGA CAGTTTCTTA 
AAACTCACTA CGCGTGCTCA ACTTGGTGCA AAATCAGAAC AGTTGCTGAA GAAAGGAAAG 
AGCATTCCTG ACGTGCTGCT TGTTGACATC ATGGTAAATG CTATTAACGA GATACCTGTG 
AATCAGCACT GGATCCTAGA TGGTTTTCCA ATGACTTTAA ATCAAGCACA GCTTCTGGAA 
GAAGCTCTTA CAGGCTACAA TAGAAACCTC ACAGAACTGG AGAGAAAAAA AGCACAAAAA 
TCCACATTGG CTATTGATCC TGCGACTTCC AAAGAAGTAC CTCTTCCCTC TCCTGCATTT 
GATTTTGTCG TATTATTAGA CGTTTCAGAT ACTTCCTCAA TGAGTCGCAT GAAGGATATA 
ATAGCTGAAG AATTGTCCTA TAAAACTGCT CACGAAGACA TCAGTCAACG TGTAGCTGCT 
GAAAATCAAG ATAAGGGTGG AGACCAAAAT TTAAGAGACC AGATACAACA TAGAATTATA 
GGCTTCTTGG ACAACTGGCC TTTATTGAAG CAATGGTTTT CAGAGCCAGA AAATATTTTG 
ATAAAAATCA ATGCTGAAAT AGATAAGGAA TCTTTATGCG AAAAAGTAAA AGAAATTCTT 
ACGACTGAAA TAGTGAAAAA AAAGAATAAA GTTGAGAAGA AATTAGAAGA AAAGGAAGCT 
GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG CTTCCACTTC CTACACCTCC TCCTGCTCCT 
CCTCCTGAAC CAGAAAAAGA GAAGGAAATT CATCTTCATC ATGTGCCTTC AAAAACTCCT 
ACTGCAAAAG GAAAACCTCA ATCAGAAGCC CCGCATGGTA AGCAAGAATC TCTTCAGGAA 
GGAAAAGGGA AGAAAGGTGA AACGGCACTC AAAAGAAAAG GTTCTCCTAA AGGAAAATCA 
TCAGGAGGAA AAGTACCAGT AAAGAAATCA CCTGCTGACT CTACAGACAC ATCACCTGTT 
GCAATAGTGC CGCAGCCACC TAAGCCAGGA TCAGAAGAAT GGGTCTATGT GAATGAACCA 
GTTCCTGAGG AAATGCCTTC GTTTTTAGTA CCTTACTGGG AACTAATAGA AAATTCCTAT 
ATAAACACCA TCAAAACAGT ACTCAGGCAT CTGAGGGAAG ACCAGCATAC TGTGCTTGCT 
TACTTATATG AGATTAGAAC AAGTTTCCAG GAGTTTCTAA AGCGTCCAGA TCACAAGCAA 
GATTTTGTAG CTCAATGGCA GGCTGATTTC AATTCCCTTC CTGATGACCT GTGGGATGAT 
GAGGAAACAA AGGCTGAACT ACACCAACGA GTGAATGATC TGCGAGACCG CCTGTGGGAC 
ATTTGTGACG CACGGAAGGA AGAGGCAGAG CAGGAGCGGC TTGACATCAT TAATGAGAAC 
TGGTTACAGG ACTCTCTTGG AATGACAATG AACCATTTCT TTTCCCTGAT GCAGGCAGAA 
CTGAACCGTT TCCAAGATAC GAAGAGACTC CTTCAAGATT ATTACCGGGG AATGGAAAGT 
AAAATCCCAA TAGAGGACAA TAAGAGATTT ACTCAAATCC CTTTGGTCCA GCTGGATAAT 
AAAGACAATT CTGAAAGCCA GCTTAGAATT CCTCTATTTC CTCGAATATC CATTTCTCTG 
GAAACAGTTA CGCCCAAACC AAAAACAAAA TCAATACTGA AGGGCAAGAT GGATAACTCC 
CTAGAAAACG TTGAGTCCAA CTTTGAGGCT GATGAAAAGT TGGTCATGGA CACCTGGCAA 
CAGGCTTCTT TAGCAGTATC TCACATGGTG GCTGCAGAAA TTCATCAGAG GCTCATGGAA 
GAAGAAAAAG AAAACCAGCC AGCAGACCCC AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT 
AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA AAACAGGAAG ACAAAAAACT CAAAGGTAAA 
TCACCACCTA TTGCAGAAGC AACTCCTGTC ATAGTAACAA CAGAGGAAAT TGCTGAAATC 
GAAAGGAAAA ATGAACTGAG GCTCAAAATA AAAGAAGAAC ACCTTGCTGC CTTGCAATTT 
GAAGAAACAG CCACACAGTT TCGACTTGAA CTGATAAAGA CAAAAGCATT GGCTCTTCTT 
GAAGATTTAG TAACAAAGGT GGTTGATGTA TATAAACTCA TGGAAAAATG GCTTGGTGAG 
AGATATTTGA ATGAAATGGC CAGTGTAGAA AAATTAACTG ACATAGCTCG CTATCACATT 
GAAACATCTA CAAAAATTCA GAATGAACTT TATTTAAACC AAGAAGACTT CTTCATTAAT 
GGCAATGTAA AAGTCTTTCC AGATCCTCCC CCACCGATAC ATCCTCCACC TGTAGAAAAG 
GAAGAAGATG GCACCCTGAC CATCGAACAG CTTGACAATC TTCGGGATCA GTTCTTAGAT 
ATGGCACCTA AAGTTACAGG AATTAACATC TTTATTAACA GTCAACTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007959551.1
CDS	107..4597
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGFLLGEVLYKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQPLTNLRLQHMKSETFRERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIKKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVANEIKKFEALIKKDLQAKESASKTTLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYKKHYSICAEILDQIVDLSTKVADYRTLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLIELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPMRILSIDTLVQEAIQAFHDNEKVSEILPIQKKDEEDALPVLQEEIKESQASDPQNVFSAGPVSNEGLPETEGETILSANADKTPKAEEIKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINEIPVNQHWILDGFPMTLNQAQLLEEALTGYNRNLTELERKKAQKSTLAIDPATSKEVPLPSPAFDFVVLLDVSDTSSMSRMKDIIAEELSYKTAHEDISQRVAAENQDKGGDQNLRDQIQHRIIGFLDNWPLLKQWFSEPENILIKINAEIDKESLCEKVKEILTTEIVKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHLHHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINENWLQDSLGMTMNHFFSLMQAELNRFQDTKRLLQDYYRGMESKIPIEDNKRFTQIPLVQLDNKDNSESQLRIPLFPRISISLETVTPKPKTKSILKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKLKGKSPPIAEATPVIVTTEEIAEIERKNELRLKIKEEHLAALQFEETATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASVEKLTDIARYHIETSTKIQNELYLNQEDFFINGNVKVFPDPPPPIHPPPVEKEEDGTLTIEQLDNLRDQFLDMAPKVTGINIFINSQL

Target ORF information:

RefSeq Version	XM_007961360.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007961360.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG CAAGTGATCC ACAAAATGTA 
TTTTCAGCTG GTCCAGTTTC AAATGAAGGA TTACCAGAAA CAGAAGGTGA AACGATACTA 
AGTGCTAATG CTGATAAAAC ACCAAAAGCT GAAGAAATCA AATCCAGTGA CAGTTTCTTA 
AAACTCACTA CGCGTGCTCA ACTTGGTGCA AAATCAGAAC AGTTGCTGAA GAAAGGAAAG 
AGCATTCCTG ACGTGCTGCT TGTTGACATC ATGGTAAATG CTATTAACGA GATACCTGTG 
AATCAGCACT GGATCCTAGA TGGTTTTCCA ATGACTTTAA ATCAAGCACA GCTTCTGGAA 
GAAGCTCTTA CAGGCTACAA TAGAAACCTC ACAGAACTGG AGAGAAAAAA AGCACAAAAA 
TCCACATTGG CTATTGATCC TGCGACTTCC AAAGAAGTAC CTCTTCCCTC TCCTGCATTT 
GATTTTGTCG TATTATTAGA CGTTTCAGAT ACTTCCTCAA TGAGTCGCAT GAAGGATATA 
ATAGCTGAAG AATTGTCCTA TAAAACTGCT CACGAAGACA TCAGTCAACG TGTAGCTGCT 
GAAAATCAAG ATAAGGGTGG AGACCAAAAT TTAAGAGACC AGATACAACA TAGAATTATA 
GGCTTCTTGG ACAACTGGCC TTTATTGAAG CAATGGTTTT CAGAGCCAGA AAATATTTTG 
ATAAAAATCA ATGCTGAAAT AGATAAGGAA TCTTTATGCG AAAAAGTAAA AGAAATTCTT 
ACGACTGAAA TAGTGAAAAA AAAGAATAAA GTTGAGAAGA AATTAGAAGA AAAGGAAGCT 
GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG CTTCCACTTC CTACACCTCC TCCTGCTCCT 
CCTCCTGAAC CAGAAAAAGA GAAGGAAATT CATCTTCATC ATGTGCCTTC AAAAACTCCT 
ACTGCAAAAG GAAAACCTCA ATCAGAAGCC CCGCATGGTA AGCAAGAATC TCTTCAGGAA 
GGAAAAGGGA AGAAAGGTGA AACGGCACTC AAAAGAAAAG GTTCTCCTAA AGGAAAATCA 
TCAGGAGGAA AAGTACCAGT AAAGAAATCA CCTGCTGACT CTACAGACAC ATCACCTGTT 
GCAATAGTGC CGCAGCCACC TAAGCCAGGA TCAGAAGAAT GGGTCTATGT GAATGAACCA 
GTTCCTGAGG AAATGCCTTC GTTTTTAGTA CCTTACTGGG AACTAATAGA AAATTCCTAT 
ATAAACACCA TCAAAACAGT ACTCAGGCAT CTGAGGGAAG ACCAGCATAC TGTGCTTGCT 
TACTTATATG AGATTAGAAC AAGTTTCCAG GAGTTTCTAA AGCGTCCAGA TCACAAGCAA 
GATTTTGTAG CTCAATGGCA GGCTGATTTC AATTCCCTTC CTGATGACCT GTGGGATGAT 
GAGGAAACAA AGGCTGAACT ACACCAACGA GTGAATGATC TGCGAGACCG CCTGTGGGAC 
ATTTGTGACG CACGGAAGGA AGAGGCAGAG CAGGAGCGGC TTGACATCAT TAATGAGAAC 
TGGTTACAGG ACTCTCTTGG AATGACAATG AACCATTTCT TTTCCCTGAT GCAGGCAGAA 
CTGAACCGTT TCCAAGATAC GAAGAGACTC CTTCAAGATT ATTACCGGGG AATGGAAAGT 
AAAATCCCAA TAGAGGACAA TAAGAGATTT ACTCAAATCC CTTTGGTCCA GCTGGATAAT 
AAAGACAATT CTGAAAGCCA GCTTAGAATT CCTCTATTTC CTCGAATATC CATTTCTCTG 
GAAACAGTTA CGCCCAAACC AAAAACAAAA TCAATACTGA AGGGCAAGAT GGATAACTCC 
CTAGAAAACG TTGAGTCCAA CTTTGAGGCT GATGAAAAGT TGGTCATGGA CACCTGGCAA 
CAGGCTTCTT TAGCAGTATC TCACATGGTG GCTGCAGAAA TTCATCAGAG GCTCATGGAA 
GAAGAAAAAG AAAACCAGCC AGCAGACCCC AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT 
AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA AAACAGGAAG ACAAAAAACT CAAAGGTAAA 
TCACCACCTA TTGCAGAAGC AACTCCTGTC ATAGTAACAA CAGAGGAAAT TGCTGAAATC 
GAAAGGAAAA ATGAACTGAG GCTCAAAATA AAAGAAGAAC ACCTTGCTGC CTTGCAATTT 
GAAGAAACAG CCACACAGTT TCGACTTGAA CTGATAAAGA CAAAAGCATT GGCTCTTCTT 
GAAGATTTAG TAACAAAGGT GGTTGATGTA TATAAACTCA TGGAAAAATG GCTTGGTGAG 
AGATATTTGA ATGAAATGGC CAGTGTAGAA AAATTAACTG ACATAGCTCG CTATCACATT 
GAAACATCTA CAAAAATTCA GAATGAACTT TATTTAAACC AAGAAGACTT CTTCATTAAT 
GGCAATGTAA AAGTCTTTCC AGATCCTCCC CCACCGATAC ATCCTCCACC TGTAGAAAAG 
GAAGAAGATG GCACCCTGAC CATCGAACAG CTTGACAATC TTCGGGATCA GTTCTTAGAT 
ATGGCACCTA AAGTTACAGG AATTAACATC TTTATTAACA GTCAACTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_007961361.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007959552.1 Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OCh83471
Clone ID Related Accession (Same CDS sequence)	XM_007961361.1
Accession Version	XM_007961361.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	sperm flagellar protein 2 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735239.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG CAAGTGATCC ACAAAATGTA 
TTTTCAGCTG GTCCAGTTTC AAATGAAGGA TTACCAGAAA CAGAAGGTGA AACGATACTA 
AGTGCTAATG CTGATAAAAC ACCAAAAGCT GAAGAAATCA AATCCAGTGA CAGTTTCTTA 
AAACTCACTA CGCGTGCTCA ACTTGGTGCA AAATCAGAAC AGTTGCTGAA GAAAGGAAAG 
AGCATTCCTG ACGTGCTGCT TGTTGACATC ATGGTAAATG CTATTAACGA GATACCTGTG 
AATCAGCACT GGATCCTAGA TGGTTTTCCA ATGACTTTAA ATCAAGCACA GCTTCTGGAA 
GAAGCTCTTA CAGGCTACAA TAGAAACCTC ACAGAACTGG AGAGAAAAAA AGCACAAAAA 
TCCACATTGG CTATTGATCC TGCGACTTCC AAAGAAGTAC CTCTTCCCTC TCCTGCATTT 
GATTTTGTCG TATTATTAGA CGTTTCAGAT ACTTCCTCAA TGAGTCGCAT GAAGGATATA 
ATAGCTGAAG AATTGTCCTA TAAAACTGCT CACGAAGACA TCAGTCAACG TGTAGCTGCT 
GAAAATCAAG ATAAGGGTGG AGACCAAAAT TTAAGAGACC AGATACAACA TAGAATTATA 
GGCTTCTTGG ACAACTGGCC TTTATTGAAG CAATGGTTTT CAGAGCCAGA AAATATTTTG 
ATAAAAATCA ATGCTGAAAT AGATAAGGAA TCTTTATGCG AAAAAGTAAA AGAAATTCTT 
ACGACTGAAA TAGTGAAAAA AAAGAATAAA GTTGAGAAGA AATTAGAAGA AAAGGAAGCT 
GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG CTTCCACTTC CTACACCTCC TCCTGCTCCT 
CCTCCTGAAC CAGAAAAAGA GAAGGAAATT CATCTTCATC ATGTGCCTTC AAAAACTCCT 
ACTGCAAAAG GAAAACCTCA ATCAGAAGCC CCGCATGGTA AGCAAGAATC TCTTCAGGAA 
GGAAAAGGGA AGAAAGGTGA AACGGCACTC AAAAGAAAAG GTTCTCCTAA AGGAAAATCA 
TCAGGAGGAA AAGTACCAGT AAAGAAATCA CCTGCTGACT CTACAGACAC ATCACCTGTT 
GCAATAGTGC CGCAGCCACC TAAGCCAGGA TCAGAAGAAT GGGTCTATGT GAATGAACCA 
GTTCCTGAGG AAATGCCTTC GTTTTTAGTA CCTTACTGGG AACTAATAGA AAATTCCTAT 
ATAAACACCA TCAAAACAGT ACTCAGGCAT CTGAGGGAAG ACCAGCATAC TGTGCTTGCT 
TACTTATATG AGATTAGAAC AAGTTTCCAG GAGTTTCTAA AGCGTCCAGA TCACAAGCAA 
GATTTTGTAG CTCAATGGCA GGCTGATTTC AATTCCCTTC CTGATGACCT GTGGGATGAT 
GAGGAAACAA AGGCTGAACT ACACCAACGA GTGAATGATC TGCGAGACCG CCTGTGGGAC 
ATTTGTGACG CACGGAAGGA AGAGGCAGAG CAGGAGCGGC TTGACATCAT TAATGAGAAC 
TGGTTACAGG ACTCTCTTGG AATGACAATG AACCATTTCT TTTCCCTGAT GCAGGCAGAA 
CTGAACCGTT TCCAAGATAC GAAGAGACTC CTTCAAGATT ATTACCGGGG AATGGAAAGT 
AAAATCCCAA TAGAGGACAA TAAGAGATTT ACTCAAATCC CTTTGGTCCA GCTGGATAAT 
AAAGACAATT CTGAAAGCCA GCTTAGAATT CCTCTATTTC CTCGAATATC CATTTCTCTG 
GAAACAGTTA CGCCCAAACC AAAAACAAAA TCAATACTGA AGGGCAAGAT GGATAACTCC 
CTAGAAAACG TTGAGTCCAA CTTTGAGGCT GATGAAAAGT TGGTCATGGA CACCTGGCAA 
CAGGCTTCTT TAGCAGTATC TCACATGGTG GCTGCAGAAA TTCATCAGAG GCTCATGGAA 
GAAGAAAAAG AAAACCAGCC AGCAGACCCC AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT 
AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA AAACAGGAAG ACAAAAAACT CAAAGGTAAA 
TCACCACCTA TTGCAGAAGC AACTCCTGTC ATAGTAACAA CAGAGGAAAT TGCTGAAATC 
GAAAGGAAAA ATGAACTGAG GCTCAAAATA AAAGAAGAAC ACCTTGCTGC CTTGCAATTT 
GAAGAAACAG CCACACAGTT TCGACTTGAA CTGATAAAGA CAAAAGCATT GGCTCTTCTT 
GAAGATTTAG TAACAAAGGT GGTTGATGTA TATAAACTCA TGGAAAAATG GCTTGGTGAG 
AGATATTTGA ATGAAATGGC CAGTGTAGAA AAATTAACTG ACATAGCTCG CTATCACATT 
GAAACATCTA CAAAAATTCA GAATGAACTT TATTTAAACC AAGAAGACTT CTTCATTAAT 
GGCAATGTAA AAGTCTTTCC AGATCCTCCC CCACCGATAC ATCCTCCACC TGTAGAAAAG 
GAAGAAGATG GCACCCTGAC CATCGAACAG CTTGACAATC TTCGGGATCA GTTCTTAGAT 
ATGGCACCTA AAGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007959552.1
CDS	107..4564
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGFLLGEVLYKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQPLTNLRLQHMKSETFRERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIKKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVANEIKKFEALIKKDLQAKESASKTTLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYKKHYSICAEILDQIVDLSTKVADYRTLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLIELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPMRILSIDTLVQEAIQAFHDNEKVSEILPIQKKDEEDALPVLQEEIKESQASDPQNVFSAGPVSNEGLPETEGETILSANADKTPKAEEIKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINEIPVNQHWILDGFPMTLNQAQLLEEALTGYNRNLTELERKKAQKSTLAIDPATSKEVPLPSPAFDFVVLLDVSDTSSMSRMKDIIAEELSYKTAHEDISQRVAAENQDKGGDQNLRDQIQHRIIGFLDNWPLLKQWFSEPENILIKINAEIDKESLCEKVKEILTTEIVKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHLHHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINENWLQDSLGMTMNHFFSLMQAELNRFQDTKRLLQDYYRGMESKIPIEDNKRFTQIPLVQLDNKDNSESQLRIPLFPRISISLETVTPKPKTKSILKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKLKGKSPPIAEATPVIVTTEEIAEIERKNELRLKIKEEHLAALQFEETATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASVEKLTDIARYHIETSTKIQNELYLNQEDFFINGNVKVFPDPPPPIHPPPVEKEEDGTLTIEQLDNLRDQFLDMAPKD

Target ORF information:

RefSeq Version	XM_007961361.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007961361.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG CAAGTGATCC ACAAAATGTA 
TTTTCAGCTG GTCCAGTTTC AAATGAAGGA TTACCAGAAA CAGAAGGTGA AACGATACTA 
AGTGCTAATG CTGATAAAAC ACCAAAAGCT GAAGAAATCA AATCCAGTGA CAGTTTCTTA 
AAACTCACTA CGCGTGCTCA ACTTGGTGCA AAATCAGAAC AGTTGCTGAA GAAAGGAAAG 
AGCATTCCTG ACGTGCTGCT TGTTGACATC ATGGTAAATG CTATTAACGA GATACCTGTG 
AATCAGCACT GGATCCTAGA TGGTTTTCCA ATGACTTTAA ATCAAGCACA GCTTCTGGAA 
GAAGCTCTTA CAGGCTACAA TAGAAACCTC ACAGAACTGG AGAGAAAAAA AGCACAAAAA 
TCCACATTGG CTATTGATCC TGCGACTTCC AAAGAAGTAC CTCTTCCCTC TCCTGCATTT 
GATTTTGTCG TATTATTAGA CGTTTCAGAT ACTTCCTCAA TGAGTCGCAT GAAGGATATA 
ATAGCTGAAG AATTGTCCTA TAAAACTGCT CACGAAGACA TCAGTCAACG TGTAGCTGCT 
GAAAATCAAG ATAAGGGTGG AGACCAAAAT TTAAGAGACC AGATACAACA TAGAATTATA 
GGCTTCTTGG ACAACTGGCC TTTATTGAAG CAATGGTTTT CAGAGCCAGA AAATATTTTG 
ATAAAAATCA ATGCTGAAAT AGATAAGGAA TCTTTATGCG AAAAAGTAAA AGAAATTCTT 
ACGACTGAAA TAGTGAAAAA AAAGAATAAA GTTGAGAAGA AATTAGAAGA AAAGGAAGCT 
GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG CTTCCACTTC CTACACCTCC TCCTGCTCCT 
CCTCCTGAAC CAGAAAAAGA GAAGGAAATT CATCTTCATC ATGTGCCTTC AAAAACTCCT 
ACTGCAAAAG GAAAACCTCA ATCAGAAGCC CCGCATGGTA AGCAAGAATC TCTTCAGGAA 
GGAAAAGGGA AGAAAGGTGA AACGGCACTC AAAAGAAAAG GTTCTCCTAA AGGAAAATCA 
TCAGGAGGAA AAGTACCAGT AAAGAAATCA CCTGCTGACT CTACAGACAC ATCACCTGTT 
GCAATAGTGC CGCAGCCACC TAAGCCAGGA TCAGAAGAAT GGGTCTATGT GAATGAACCA 
GTTCCTGAGG AAATGCCTTC GTTTTTAGTA CCTTACTGGG AACTAATAGA AAATTCCTAT 
ATAAACACCA TCAAAACAGT ACTCAGGCAT CTGAGGGAAG ACCAGCATAC TGTGCTTGCT 
TACTTATATG AGATTAGAAC AAGTTTCCAG GAGTTTCTAA AGCGTCCAGA TCACAAGCAA 
GATTTTGTAG CTCAATGGCA GGCTGATTTC AATTCCCTTC CTGATGACCT GTGGGATGAT 
GAGGAAACAA AGGCTGAACT ACACCAACGA GTGAATGATC TGCGAGACCG CCTGTGGGAC 
ATTTGTGACG CACGGAAGGA AGAGGCAGAG CAGGAGCGGC TTGACATCAT TAATGAGAAC 
TGGTTACAGG ACTCTCTTGG AATGACAATG AACCATTTCT TTTCCCTGAT GCAGGCAGAA 
CTGAACCGTT TCCAAGATAC GAAGAGACTC CTTCAAGATT ATTACCGGGG AATGGAAAGT 
AAAATCCCAA TAGAGGACAA TAAGAGATTT ACTCAAATCC CTTTGGTCCA GCTGGATAAT 
AAAGACAATT CTGAAAGCCA GCTTAGAATT CCTCTATTTC CTCGAATATC CATTTCTCTG 
GAAACAGTTA CGCCCAAACC AAAAACAAAA TCAATACTGA AGGGCAAGAT GGATAACTCC 
CTAGAAAACG TTGAGTCCAA CTTTGAGGCT GATGAAAAGT TGGTCATGGA CACCTGGCAA 
CAGGCTTCTT TAGCAGTATC TCACATGGTG GCTGCAGAAA TTCATCAGAG GCTCATGGAA 
GAAGAAAAAG AAAACCAGCC AGCAGACCCC AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT 
AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA AAACAGGAAG ACAAAAAACT CAAAGGTAAA 
TCACCACCTA TTGCAGAAGC AACTCCTGTC ATAGTAACAA CAGAGGAAAT TGCTGAAATC 
GAAAGGAAAA ATGAACTGAG GCTCAAAATA AAAGAAGAAC ACCTTGCTGC CTTGCAATTT 
GAAGAAACAG CCACACAGTT TCGACTTGAA CTGATAAAGA CAAAAGCATT GGCTCTTCTT 
GAAGATTTAG TAACAAAGGT GGTTGATGTA TATAAACTCA TGGAAAAATG GCTTGGTGAG 
AGATATTTGA ATGAAATGGC CAGTGTAGAA AAATTAACTG ACATAGCTCG CTATCACATT 
GAAACATCTA CAAAAATTCA GAATGAACTT TATTTAAACC AAGAAGACTT CTTCATTAAT 
GGCAATGTAA AAGTCTTTCC AGATCCTCCC CCACCGATAC ATCCTCCACC TGTAGAAAAG 
GAAGAAGATG GCACCCTGAC CATCGAACAG CTTGACAATC TTCGGGATCA GTTCTTAGAT 
ATGGCACCTA AAGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_007961353.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007959544.1 Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X7, mRNA.

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CloneID	OCh83463
Clone ID Related Accession (Same CDS sequence)	XM_007961353.1
Accession Version	XM_007961353.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	sperm flagellar protein 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735239.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG CAAGTGATCC ACAAAATGTA 
TTTTCAGCTG GTCCAGTTTC AAATGAAGGA TTACCAGAAA CAGAAGGTGA AACGATACTA 
AGTGCTAATG CTGATAAAAC ACCAAAAGCT GAAGAAATCA AATCCAGTGA CAGTTTCTTA 
AAACTCACTA CGCGTGCTCA ACTTGGTGCA AAATCAGAAC AGTTGCTGAA GAAAGGAAAG 
AGCATTCCTG ACGTGCTGCT TGTTGACATC ATGGTAAATG CTATTAACGA GATACCTGTG 
AATCAGCACT GGATCCTAGA TGGTTTTCCA ATGACTTTAA ATCAAGCACA GCTTCTGGAA 
GAAGCTCTTA CAGGCTACAA TAGAAACCTC ACAGAACTGG AGAGAAAAAA AGCACAAAAA 
TCCACATTGG CTATTGATCC TGCGACTTCC AAAGAAGTAC CTCTTCCCTC TCCTGCATTT 
GATTTTGTCG TATTATTAGA CGTTTCAGAT ACTTCCTCAA TGAGTCGCAT GAAGGATATA 
ATAGCTGAAG AATTGTCCTA TAAAACTGCT CACGAAGACA TCAGTCAACG TGTAGCTGCT 
GAAAATCAAG ATAAGGGTGG AGACCAAAAT TTAAGAGACC AGATACAACA TAGAATTATA 
GGCTTCTTGG ACAACTGGCC TTTATTGAAG CAATGGTTTT CAGAGCCAGA AAATATTTTG 
ATAAAAATCA ATGCTGAAAT AGATAAGGAA TCTTTATGCG AAAAAGTAAA AGAAATTCTT 
ACGACTGAAA TAGTGAAAAA AAAGAATAAA GTTGAGAAGA AATTAGAAGA AAAGGAAGCT 
GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG CTTCCACTTC CTACACCTCC TCCTGCTCCT 
CCTCCTGAAC CAGAAAAAGA GAAGGAAATT CATCTTCATC ATGTGCCTTC AAAAACTCCT 
ACTGCAAAAG GAAAACCTCA ATCAGAAGCC CCGCATGGTA AGCAAGAATC TCTTCAGGAA 
GGAAAAGGGA AGAAAGGTGA AACGGCACTC AAAAGAAAAG GTTCTCCTAA AGGAAAATCA 
TCAGGAGGAA AAGTACCAGT AAAGAAATCA CCTGCTGACT CTACAGACAC ATCACCTGTT 
GCAATAGTGC CGCAGCCACC TAAGCCAGGA TCAGAAGAAT GGGTCTATGT GAATGAACCA 
GTTCCTGAGG AAATGCCTTC GTTTTTAGTA CCTTACTGGG AACTAATAGA AAATTCCTAT 
ATAAACACCA TCAAAACAGT ACTCAGGCAT CTGAGGGAAG ACCAGCATAC TGTGCTTGCT 
TACTTATATG AGATTAGAAC AAGTTTCCAG GAGTTTCTAA AGCGTCCAGA TCACAAGCAA 
GATTTTGTAG CTCAATGGCA GGCTGATTTC AATTCCCTTC CTGATGACCT GTGGGATGAT 
GAGGAAACAA AGGCTGAACT ACACCAACGA GTGAATGATC TGCGAGACCG CCTGTGGGAC 
ATTTGTGACG CACGGAAGGA AGAGGCAGAG CAGGAGCGGC TTGACATCAT TAATGAGAAC 
TGGTTACAGG ACTCTCTTGG AATGACAATG AACCATTTCT TTTCCCTGAT GCAGGCAGAA 
CTGAACCGTT TCCAAGATAC GAAGAGACTC CTTCAAGATT ATTACCGGGG AATGGAAAGT 
AAAATCCCAA TAGAGGACAA TAAGAGATTT ACTCAAATCC CTTTGGTCCA GCTGGATAAT 
AAAGACAATT CTGAAAGCCA GCTTAGAATT CCTCTATTTC CTCGAATATC CATTTCTCTG 
GAAACAGTTA CGCCCAAACC AAAAACAAAA TCAATACTGA AGGGCAAGAT GGATAACTCC 
CTAGAAAACG TTGAGTCCAA CTTTGAGGCT GATGAAAAGT TGGTCATGGA CACCTGGCAA 
CAGGCTTCTT TAGCAGTATC TCACATGGTG GCTGCAGAAA TTCATCAGAG GCTCATGGAA 
GAAGAAAAAG AAAACCAGCC AGCAGACCCC AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT 
AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA AAACAGGAAG ACAAAAAACT CAAAGGTAAA 
TCACCACCTA TTGCAGAAGC AACTCCTGTC ATAGTAACAA CAGAGGAAAT TGCTGAAATC 
GAAAGGAAAA ATGAACTGAG GCTCAAAATA AAAGAAGAAC ACCTTGCTGC CTTGCAATTT 
GAAGAAACAG CCACACAGTT TCGACTTGAA CTGATAAAGA CAAAAGCATT GGCTCTTCTT 
GAAGATTTAG TAACAAAGGT GGTTGATGTA TATAAACTCA TGGAAAAATG GCTTGGTGAG 
AGATATTTGA ATGAAATGGC CAGTGTAGAA AAATTAACTG ACATAGCTCG CTATCACATT 
GAAACATCTA CAAAAATTCA GAATGAACTT TATTTAAACC AAGAAGACTT CTTCATTAAT 
GGCAATGTAA AAGTCTTTCC AGATCCTCCC CCACCGATAC ATCCTCCACC TGTAGAAAAG 
GAAGAAGATG GCACCCTGAC CATCGAACAG CTTGACAATC TTCGGGATCA GTTCTTAGAT 
ATGGCACCTA AAGGCATAAT AGGAAATAAA GCATTTACTG ACATTCTGAT CGATTTGGTG 
ACCCTGAACC TTGGCACAAA CAACTTTCCT AGTAGTTGGA TGCACCTTAC CCAACCTGAA 
TTACAGGAAT TAACATCTTT ATTAACAGTC AACTCTGAGT TTGTGGACTG GCGGAAGTTC 
CTGTTAGTAA CCTCAATGCC TTGGCCCATT CCCTTGGAGG AGGAACTCCT TGAGACCCTT 
CAGAAATTCA AGGCTGTGGA TAAGGCGCAG TTGGGCACCA TCACTTTCGA GCAGTATATG 
CAGGCTGGTC TGTGGTTTAC AGGAGATGAA GATATAAAAA TTCCAGACAA TCCTCTTGAA 
CCCCTGCCAT TTAATAGGCA GGAGCATCTT ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC 
TATGAGAAGG ATCCACCCCA ACTTGACTAC ACACAGATGC TGCTTTACTT TGCTTGCCAC 
CCAGACACCG TGGAAGGAGT CTACAGGGCC CTCAGTGTGG CTGTTGGGAC TCATGTCTTC 
CAACAAGTTG AAGCTTCCAC TCCAAATGCA GAAAAGACCT CCTCAACTGA TGCAAGTCCA 
GCTGAGGAAT TCCCTGAACC TGAGGAAAAT GCTGCAAAAG AAGAAAGGAA ATTAAAAGAC 
AACAGGGAGG AAAGGGAACA GAAGGATGAA GAAATCCCTG AAAATGCAAA CAATGAAAAG 
ATATCCATGG AAACATTACT CAAAGTGTTC AAAGGGGGAA GTGAAGCACA GGACTCTAAC 
AGATTTGCCA GCCACCTAAA GATAGAGAAC ATTTATGCAG AGGGCTTCAT AAAAACATTT 
CAAGACCTAG GTGCCAAGAA CCTGGAGCCA ATTGAAGTTG CTGTTCTCTT GAAGCATCCT 
TTTATTCAAG ACCTGATTTC AAATTATTCA GACTATAAGT TTCCTGTGAG TATTACCCCA 
AAGTGCTCTA ATGTGGTTGG GCTCCGGTTC AGGACATGCT ATGATGTTTC TAAATGTGAG 
CTGGCATGGA AGAGAAAGAA ACTGGACTAT GCCAGGCCAG GCCAGGCCAG AATCTGGAGA 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007959544.1
CDS	107..5632
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGFLLGEVLYKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQPLTNLRLQHMKSETFRERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIKKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVANEIKKFEALIKKDLQAKESASKTTLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYKKHYSICAEILDQIVDLSTKVADYRTLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLIELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPMRILSIDTLVQEAIQAFHDNEKVSEILPIQKKDEEDALPVLQEEIKESQASDPQNVFSAGPVSNEGLPETEGETILSANADKTPKAEEIKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINEIPVNQHWILDGFPMTLNQAQLLEEALTGYNRNLTELERKKAQKSTLAIDPATSKEVPLPSPAFDFVVLLDVSDTSSMSRMKDIIAEELSYKTAHEDISQRVAAENQDKGGDQNLRDQIQHRIIGFLDNWPLLKQWFSEPENILIKINAEIDKESLCEKVKEILTTEIVKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHLHHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINENWLQDSLGMTMNHFFSLMQAELNRFQDTKRLLQDYYRGMESKIPIEDNKRFTQIPLVQLDNKDNSESQLRIPLFPRISISLETVTPKPKTKSILKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKLKGKSPPIAEATPVIVTTEEIAEIERKNELRLKIKEEHLAALQFEETATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASVEKLTDIARYHIETSTKIQNELYLNQEDFFINGNVKVFPDPPPPIHPPPVEKEEDGTLTIEQLDNLRDQFLDMAPKGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKAQLGTITFEQYMQAGLWFTGDEDIKIPDNPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVEASTPNAEKTSSTDASPAEEFPEPEENAAKEERKLKDNREEREQKDEEIPENANNEKISMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVSITPKCSNVVGLRFRTCYDVSKCELAWKRKKLDYARPGQARIWRS

Target ORF information:

RefSeq Version	XM_007961353.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007961353.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG CAAGTGATCC ACAAAATGTA 
TTTTCAGCTG GTCCAGTTTC AAATGAAGGA TTACCAGAAA CAGAAGGTGA AACGATACTA 
AGTGCTAATG CTGATAAAAC ACCAAAAGCT GAAGAAATCA AATCCAGTGA CAGTTTCTTA 
AAACTCACTA CGCGTGCTCA ACTTGGTGCA AAATCAGAAC AGTTGCTGAA GAAAGGAAAG 
AGCATTCCTG ACGTGCTGCT TGTTGACATC ATGGTAAATG CTATTAACGA GATACCTGTG 
AATCAGCACT GGATCCTAGA TGGTTTTCCA ATGACTTTAA ATCAAGCACA GCTTCTGGAA 
GAAGCTCTTA CAGGCTACAA TAGAAACCTC ACAGAACTGG AGAGAAAAAA AGCACAAAAA 
TCCACATTGG CTATTGATCC TGCGACTTCC AAAGAAGTAC CTCTTCCCTC TCCTGCATTT 
GATTTTGTCG TATTATTAGA CGTTTCAGAT ACTTCCTCAA TGAGTCGCAT GAAGGATATA 
ATAGCTGAAG AATTGTCCTA TAAAACTGCT CACGAAGACA TCAGTCAACG TGTAGCTGCT 
GAAAATCAAG ATAAGGGTGG AGACCAAAAT TTAAGAGACC AGATACAACA TAGAATTATA 
GGCTTCTTGG ACAACTGGCC TTTATTGAAG CAATGGTTTT CAGAGCCAGA AAATATTTTG 
ATAAAAATCA ATGCTGAAAT AGATAAGGAA TCTTTATGCG AAAAAGTAAA AGAAATTCTT 
ACGACTGAAA TAGTGAAAAA AAAGAATAAA GTTGAGAAGA AATTAGAAGA AAAGGAAGCT 
GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG CTTCCACTTC CTACACCTCC TCCTGCTCCT 
CCTCCTGAAC CAGAAAAAGA GAAGGAAATT CATCTTCATC ATGTGCCTTC AAAAACTCCT 
ACTGCAAAAG GAAAACCTCA ATCAGAAGCC CCGCATGGTA AGCAAGAATC TCTTCAGGAA 
GGAAAAGGGA AGAAAGGTGA AACGGCACTC AAAAGAAAAG GTTCTCCTAA AGGAAAATCA 
TCAGGAGGAA AAGTACCAGT AAAGAAATCA CCTGCTGACT CTACAGACAC ATCACCTGTT 
GCAATAGTGC CGCAGCCACC TAAGCCAGGA TCAGAAGAAT GGGTCTATGT GAATGAACCA 
GTTCCTGAGG AAATGCCTTC GTTTTTAGTA CCTTACTGGG AACTAATAGA AAATTCCTAT 
ATAAACACCA TCAAAACAGT ACTCAGGCAT CTGAGGGAAG ACCAGCATAC TGTGCTTGCT 
TACTTATATG AGATTAGAAC AAGTTTCCAG GAGTTTCTAA AGCGTCCAGA TCACAAGCAA 
GATTTTGTAG CTCAATGGCA GGCTGATTTC AATTCCCTTC CTGATGACCT GTGGGATGAT 
GAGGAAACAA AGGCTGAACT ACACCAACGA GTGAATGATC TGCGAGACCG CCTGTGGGAC 
ATTTGTGACG CACGGAAGGA AGAGGCAGAG CAGGAGCGGC TTGACATCAT TAATGAGAAC 
TGGTTACAGG ACTCTCTTGG AATGACAATG AACCATTTCT TTTCCCTGAT GCAGGCAGAA 
CTGAACCGTT TCCAAGATAC GAAGAGACTC CTTCAAGATT ATTACCGGGG AATGGAAAGT 
AAAATCCCAA TAGAGGACAA TAAGAGATTT ACTCAAATCC CTTTGGTCCA GCTGGATAAT 
AAAGACAATT CTGAAAGCCA GCTTAGAATT CCTCTATTTC CTCGAATATC CATTTCTCTG 
GAAACAGTTA CGCCCAAACC AAAAACAAAA TCAATACTGA AGGGCAAGAT GGATAACTCC 
CTAGAAAACG TTGAGTCCAA CTTTGAGGCT GATGAAAAGT TGGTCATGGA CACCTGGCAA 
CAGGCTTCTT TAGCAGTATC TCACATGGTG GCTGCAGAAA TTCATCAGAG GCTCATGGAA 
GAAGAAAAAG AAAACCAGCC AGCAGACCCC AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT 
AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA AAACAGGAAG ACAAAAAACT CAAAGGTAAA 
TCACCACCTA TTGCAGAAGC AACTCCTGTC ATAGTAACAA CAGAGGAAAT TGCTGAAATC 
GAAAGGAAAA ATGAACTGAG GCTCAAAATA AAAGAAGAAC ACCTTGCTGC CTTGCAATTT 
GAAGAAACAG CCACACAGTT TCGACTTGAA CTGATAAAGA CAAAAGCATT GGCTCTTCTT 
GAAGATTTAG TAACAAAGGT GGTTGATGTA TATAAACTCA TGGAAAAATG GCTTGGTGAG 
AGATATTTGA ATGAAATGGC CAGTGTAGAA AAATTAACTG ACATAGCTCG CTATCACATT 
GAAACATCTA CAAAAATTCA GAATGAACTT TATTTAAACC AAGAAGACTT CTTCATTAAT 
GGCAATGTAA AAGTCTTTCC AGATCCTCCC CCACCGATAC ATCCTCCACC TGTAGAAAAG 
GAAGAAGATG GCACCCTGAC CATCGAACAG CTTGACAATC TTCGGGATCA GTTCTTAGAT 
ATGGCACCTA AAGGCATAAT AGGAAATAAA GCATTTACTG ACATTCTGAT CGATTTGGTG 
ACCCTGAACC TTGGCACAAA CAACTTTCCT AGTAGTTGGA TGCACCTTAC CCAACCTGAA 
TTACAGGAAT TAACATCTTT ATTAACAGTC AACTCTGAGT TTGTGGACTG GCGGAAGTTC 
CTGTTAGTAA CCTCAATGCC TTGGCCCATT CCCTTGGAGG AGGAACTCCT TGAGACCCTT 
CAGAAATTCA AGGCTGTGGA TAAGGCGCAG TTGGGCACCA TCACTTTCGA GCAGTATATG 
CAGGCTGGTC TGTGGTTTAC AGGAGATGAA GATATAAAAA TTCCAGACAA TCCTCTTGAA 
CCCCTGCCAT TTAATAGGCA GGAGCATCTT ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC 
TATGAGAAGG ATCCACCCCA ACTTGACTAC ACACAGATGC TGCTTTACTT TGCTTGCCAC 
CCAGACACCG TGGAAGGAGT CTACAGGGCC CTCAGTGTGG CTGTTGGGAC TCATGTCTTC 
CAACAAGTTG AAGCTTCCAC TCCAAATGCA GAAAAGACCT CCTCAACTGA TGCAAGTCCA 
GCTGAGGAAT TCCCTGAACC TGAGGAAAAT GCTGCAAAAG AAGAAAGGAA ATTAAAAGAC 
AACAGGGAGG AAAGGGAACA GAAGGATGAA GAAATCCCTG AAAATGCAAA CAATGAAAAG 
ATATCCATGG AAACATTACT CAAAGTGTTC AAAGGGGGAA GTGAAGCACA GGACTCTAAC 
AGATTTGCCA GCCACCTAAA GATAGAGAAC ATTTATGCAG AGGGCTTCAT AAAAACATTT 
CAAGACCTAG GTGCCAAGAA CCTGGAGCCA ATTGAAGTTG CTGTTCTCTT GAAGCATCCT 
TTTATTCAAG ACCTGATTTC AAATTATTCA GACTATAAGT TTCCTGTGAG TATTACCCCA 
AAGTGCTCTA ATGTGGTTGG GCTCCGGTTC AGGACATGCT ATGATGTTTC TAAATGTGAG 
CTGGCATGGA AGAGAAAGAA ACTGGACTAT GCCAGGCCAG GCCAGGCCAG AATCTGGAGA 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_007961354.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007959545.1 Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X8, mRNA.

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CloneID	OCh83464
Clone ID Related Accession (Same CDS sequence)	XM_007961354.1
Accession Version	XM_007961354.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	sperm flagellar protein 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735239.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAAAA TGTATTTTCA 
GCTGGTCCAG TTTCAAATGA AGGATTACCA GAAACAGAAG GTGCTAATGC TGATAAAACA 
CCAAAAGCTG AAGAAATCAA ATCCAGTGAC AGTTTCTTAA AACTCACTAC GCGTGCTCAA 
CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA CGTGCTGCTT 
GTTGACATCA TGGTAAATGC TATTAACGAG ATACCTGTGA ATCAGCACTG GATCCTAGAT 
GGTTTTCCAA TGACTTTAAA TCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTACAAT 
AGAAACCTCA CAGAACTGGA GAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT 
GCGACTTCCA AAGAAGTACC TCTTCCCTCT CCTGCATTTG ATTTTGTCGT ATTATTAGAC 
GTTTCAGATA CTTCCTCAAT GAGTCGCATG AAGGATATAA TAGCTGAAGA ATTGTCCTAT 
AAAACTGCTC ACGAAGACAT CAGTCAACGT GTAGCTGCTG AAAATCAAGA TAAGGGTGGA 
GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT 
TTATTGAAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA 
GATAAGGAAT CTTTATGCGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGTGAAAAAA 
AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC 
CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG 
AAGGAAATTC ATCTTCATCA TGTGCCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA 
TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAA 
ACGGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA 
AAGAAATCAC CTGCTGACTC TACAGACACA TCACCTGTTG CAATAGTGCC GCAGCCACCT 
AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTCG 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA 
CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA 
AGTTTCCAGG AGTTTCTAAA GCGTCCAGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ATTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA 
CACCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGACGC ACGGAAGGAA 
GAGGCAGAGC AGGAGCGGCT TGACATCATT AATGAGAACT GGTTACAGGA CTCTCTTGGA 
ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAAC TGAACCGTTT CCAAGATACG 
AAGAGACTCC TTCAAGATTA TTACCGGGGA ATGGAAAGTA AAATCCCAAT AGAGGACAAT 
AAGAGATTTA CTCAAATCCC TTTGGTCCAG CTGGATAATA AAGACAATTC TGAAAGCCAG 
CTTAGAATTC CTCTATTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC GCCCAAACCA 
AAAACAAAAT CAATACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC 
TTTGAGGCTG ATGAAAAGTT GGTCATGGAC ACCTGGCAAC AGGCTTCTTT AGCAGTATCT 
CACATGGTGG CTGCAGAAAT TCATCAGAGG CTCATGGAAG AAGAAAAAGA AAACCAGCCA 
GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA 
CCCAAGAAAA AACAGGAAGA CAAAAAACTC AAAGGTAAAT CACCACCTAT TGCAGAAGCA 
ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCG AAAGGAAAAA TGAACTGAGG 
CTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAACAGC CACACAGTTT 
CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG 
GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GATATTTGAA TGAAATGGCC 
AGTGTAGAAA AATTAACTGA CATAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG 
AATGAACTTT ATTTAAACCA AGAAGACTTC TTCATTAATG GCAATGTAAA AGTCTTTCCA 
GATCCTCCCC CACCGATACA TCCTCCACCT GTAGAAAAGG AAGAAGATGG CACCCTGACC 
ATCGAACAGC TTGACAATCT TCGGGATCAG TTCTTAGATA TGGCACCTAA AGGCATAATA 
GGAAATAAAG CATTTACTGA CATTCTGATC GATTTGGTGA CCCTGAACCT TGGCACAAAC 
AACTTTCCTA GTAGTTGGAT GCACCTTACC CAACCTGAAT TACAGGAATT AACATCTTTA 
TTAACAGTCA ACTCTGAGTT TGTGGACTGG CGGAAGTTCC TGTTAGTAAC CTCAATGCCT 
TGGCCCATTC CCTTGGAGGA GGAACTCCTT GAGACCCTTC AGAAATTCAA GGCTGTGGAT 
AAGGCGCAGT TGGGCACCAT CACTTTCGAG CAGTATATGC AGGCTGGTCT GTGGTTTACA 
GGAGATGAAG ATATAAAAAT TCCAGACAAT CCTCTTGAAC CCCTGCCATT TAATAGGCAG 
GAGCATCTTA TAGAGTTTTT CTTTAGGCTA TTTGCTGACT ATGAGAAGGA TCCACCCCAA 
CTTGACTACA CACAGATGCT GCTTTACTTT GCTTGCCACC CAGACACCGT GGAAGGAGTC 
TACAGGGCCC TCAGTGTGGC TGTTGGGACT CATGTCTTCC AACAAGTTGA AGCTTCCACT 
CCAAATGCAG AAAAGACCTC CTCAACTGAT GCAAGTCCAG CTGAGGAATT CCCTGAACCT 
GAGGAAAATG CTGCAAAAGA AGAAAGGAAA TTAAAAGACA ACAGGGAGGA AAGGGAACAG 
AAGGATGAAG AAATCCCTGA AAATGCAAAC AATGAAAAGA TATCCATGGA AACATTACTC 
AAAGTGTTCA AAGGGGGAAG TGAAGCACAG GACTCTAACA GATTTGCCAG CCACCTAAAG 
ATAGAGAACA TTTATGCAGA GGGCTTCATA AAAACATTTC AAGACCTAGG TGCCAAGAAC 
CTGGAGCCAA TTGAAGTTGC TGTTCTCTTG AAGCATCCTT TTATTCAAGA CCTGATTTCA 
AATTATTCAG ACTATAAGTT TCCTGTGAGT ATTACCCCAA AGTGCTCTAA TGTGGTTGGG 
CTCCGGTTCA GGACATGCTA TGATGTTTCT AAATGTGAGC TGGCATGGAA GAGAAAGAAA 
CTGGACTATG CCAGGCCAGG CCAGGCCAGA ATCTGGAGAA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007959545.1
CDS	107..5611
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGFLLGEVLYKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQPLTNLRLQHMKSETFRERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIKKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVANEIKKFEALIKKDLQAKESASKTTLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYKKHYSICAEILDQIVDLSTKVADYRTLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLIELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPMRILSIDTLVQEAIQAFHDNEKVSEILPIQKKDEEDALPVLQEEIKESQDPQNVFSAGPVSNEGLPETEGANADKTPKAEEIKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINEIPVNQHWILDGFPMTLNQAQLLEEALTGYNRNLTELERKKAQKSTLAIDPATSKEVPLPSPAFDFVVLLDVSDTSSMSRMKDIIAEELSYKTAHEDISQRVAAENQDKGGDQNLRDQIQHRIIGFLDNWPLLKQWFSEPENILIKINAEIDKESLCEKVKEILTTEIVKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHLHHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINENWLQDSLGMTMNHFFSLMQAELNRFQDTKRLLQDYYRGMESKIPIEDNKRFTQIPLVQLDNKDNSESQLRIPLFPRISISLETVTPKPKTKSILKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKLKGKSPPIAEATPVIVTTEEIAEIERKNELRLKIKEEHLAALQFEETATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASVEKLTDIARYHIETSTKIQNELYLNQEDFFINGNVKVFPDPPPPIHPPPVEKEEDGTLTIEQLDNLRDQFLDMAPKGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKAQLGTITFEQYMQAGLWFTGDEDIKIPDNPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVEASTPNAEKTSSTDASPAEEFPEPEENAAKEERKLKDNREEREQKDEEIPENANNEKISMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVSITPKCSNVVGLRFRTCYDVSKCELAWKRKKLDYARPGQARIWRS

Target ORF information:

RefSeq Version	XM_007961354.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007961354.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAAAA TGTATTTTCA 
GCTGGTCCAG TTTCAAATGA AGGATTACCA GAAACAGAAG GTGCTAATGC TGATAAAACA 
CCAAAAGCTG AAGAAATCAA ATCCAGTGAC AGTTTCTTAA AACTCACTAC GCGTGCTCAA 
CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA CGTGCTGCTT 
GTTGACATCA TGGTAAATGC TATTAACGAG ATACCTGTGA ATCAGCACTG GATCCTAGAT 
GGTTTTCCAA TGACTTTAAA TCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTACAAT 
AGAAACCTCA CAGAACTGGA GAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT 
GCGACTTCCA AAGAAGTACC TCTTCCCTCT CCTGCATTTG ATTTTGTCGT ATTATTAGAC 
GTTTCAGATA CTTCCTCAAT GAGTCGCATG AAGGATATAA TAGCTGAAGA ATTGTCCTAT 
AAAACTGCTC ACGAAGACAT CAGTCAACGT GTAGCTGCTG AAAATCAAGA TAAGGGTGGA 
GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT 
TTATTGAAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA 
GATAAGGAAT CTTTATGCGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGTGAAAAAA 
AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC 
CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG 
AAGGAAATTC ATCTTCATCA TGTGCCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA 
TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAA 
ACGGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA 
AAGAAATCAC CTGCTGACTC TACAGACACA TCACCTGTTG CAATAGTGCC GCAGCCACCT 
AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTCG 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA 
CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA 
AGTTTCCAGG AGTTTCTAAA GCGTCCAGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ATTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA 
CACCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGACGC ACGGAAGGAA 
GAGGCAGAGC AGGAGCGGCT TGACATCATT AATGAGAACT GGTTACAGGA CTCTCTTGGA 
ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAAC TGAACCGTTT CCAAGATACG 
AAGAGACTCC TTCAAGATTA TTACCGGGGA ATGGAAAGTA AAATCCCAAT AGAGGACAAT 
AAGAGATTTA CTCAAATCCC TTTGGTCCAG CTGGATAATA AAGACAATTC TGAAAGCCAG 
CTTAGAATTC CTCTATTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC GCCCAAACCA 
AAAACAAAAT CAATACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC 
TTTGAGGCTG ATGAAAAGTT GGTCATGGAC ACCTGGCAAC AGGCTTCTTT AGCAGTATCT 
CACATGGTGG CTGCAGAAAT TCATCAGAGG CTCATGGAAG AAGAAAAAGA AAACCAGCCA 
GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA 
CCCAAGAAAA AACAGGAAGA CAAAAAACTC AAAGGTAAAT CACCACCTAT TGCAGAAGCA 
ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCG AAAGGAAAAA TGAACTGAGG 
CTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAACAGC CACACAGTTT 
CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG 
GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GATATTTGAA TGAAATGGCC 
AGTGTAGAAA AATTAACTGA CATAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG 
AATGAACTTT ATTTAAACCA AGAAGACTTC TTCATTAATG GCAATGTAAA AGTCTTTCCA 
GATCCTCCCC CACCGATACA TCCTCCACCT GTAGAAAAGG AAGAAGATGG CACCCTGACC 
ATCGAACAGC TTGACAATCT TCGGGATCAG TTCTTAGATA TGGCACCTAA AGGCATAATA 
GGAAATAAAG CATTTACTGA CATTCTGATC GATTTGGTGA CCCTGAACCT TGGCACAAAC 
AACTTTCCTA GTAGTTGGAT GCACCTTACC CAACCTGAAT TACAGGAATT AACATCTTTA 
TTAACAGTCA ACTCTGAGTT TGTGGACTGG CGGAAGTTCC TGTTAGTAAC CTCAATGCCT 
TGGCCCATTC CCTTGGAGGA GGAACTCCTT GAGACCCTTC AGAAATTCAA GGCTGTGGAT 
AAGGCGCAGT TGGGCACCAT CACTTTCGAG CAGTATATGC AGGCTGGTCT GTGGTTTACA 
GGAGATGAAG ATATAAAAAT TCCAGACAAT CCTCTTGAAC CCCTGCCATT TAATAGGCAG 
GAGCATCTTA TAGAGTTTTT CTTTAGGCTA TTTGCTGACT ATGAGAAGGA TCCACCCCAA 
CTTGACTACA CACAGATGCT GCTTTACTTT GCTTGCCACC CAGACACCGT GGAAGGAGTC 
TACAGGGCCC TCAGTGTGGC TGTTGGGACT CATGTCTTCC AACAAGTTGA AGCTTCCACT 
CCAAATGCAG AAAAGACCTC CTCAACTGAT GCAAGTCCAG CTGAGGAATT CCCTGAACCT 
GAGGAAAATG CTGCAAAAGA AGAAAGGAAA TTAAAAGACA ACAGGGAGGA AAGGGAACAG 
AAGGATGAAG AAATCCCTGA AAATGCAAAC AATGAAAAGA TATCCATGGA AACATTACTC 
AAAGTGTTCA AAGGGGGAAG TGAAGCACAG GACTCTAACA GATTTGCCAG CCACCTAAAG 
ATAGAGAACA TTTATGCAGA GGGCTTCATA AAAACATTTC AAGACCTAGG TGCCAAGAAC 
CTGGAGCCAA TTGAAGTTGC TGTTCTCTTG AAGCATCCTT TTATTCAAGA CCTGATTTCA 
AATTATTCAG ACTATAAGTT TCCTGTGAGT ATTACCCCAA AGTGCTCTAA TGTGGTTGGG 
CTCCGGTTCA GGACATGCTA TGATGTTTCT AAATGTGAGC TGGCATGGAA GAGAAAGAAA 
CTGGACTATG CCAGGCCAGG CCAGGCCAGA ATCTGGAGAA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_007961355.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007959546.1 Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X9, mRNA.

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CloneID	OCh83465
Clone ID Related Accession (Same CDS sequence)	XM_007961355.1
Accession Version	XM_007961355.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	sperm flagellar protein 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735239.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG CAAGTGATCC ACAAAATGTA 
TTTTCAGCTG GTCCAGTTTC AAATGAAGGA TTACCAGAAA CAGAAGGTGA AACGATACTA 
AGTGCTAATG CTGATAAAAC ACCAAAAGCT GAAGAAATCA AATCCAGTGA CAGTTTCTTA 
AAACTCACTA CGCGTGCTCA ACTTGGTGCA AAATCAGAAC AGTTGCTGAA GAAAGGAAAG 
AGCATTCCTG ACGTGCTGCT TGTTGACATC ATGGTAAATG CTATTAACGA GATACCTGTG 
AATCAGCACT GGATCCTAGA TGGTTTTCCA ATGACTTTAA ATCAAGCACA GCTTCTGGAA 
GAAGCTCTTA CAGGCTACAA TAGAAACCTC ACAGAACTGG AGAGAAAAAA AGCACAAAAA 
TCCACATTGG CTATTGATCC TGCGACTTCC AAAGAAGTAC CTCTTCCCTC TCCTGCATTT 
GATTTTGTCG TATTATTAGA CGTTTCAGAT ACTTCCTCAA TGAGTCGCAT GAAGGATATA 
ATAGCTGAAG AATTGTCCTA TAAAACTGCT CACGAAGACA TCAGTCAACG TGTAGCTGCT 
GAAAATCAAG ATAAGGGTGG AGACCAAAAT TTAAGAGACC AGATACAACA TAGAATTATA 
GGCTTCTTGG ACAACTGGCC TTTATTGAAG CAATGGTTTT CAGAGCCAGA AAATATTTTG 
ATAAAAATCA ATGCTGAAAT AGATAAGGAA TCTTTATGCG AAAAAGTAAA AGAAATTCTT 
ACGACTGAAA TAGTGAAAAA AAAGAATAAA GTTGAGAAGA AATTAGAAGA AAAGGAAGCT 
GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG CTTCCACTTC CTACACCTCC TCCTGCTCCT 
CCTCCTGAAC CAGAAAAAGA GAAGGAAATT CATCTTCATC ATGTGCCTTC AAAAACTCCT 
ACTGCAAAAG GAAAACCTCA ATCAGAAGCC CCGCATGGTA AGCAAGAATC TCTTCAGGAA 
GGAAAAGGGA AGAAAGGTGA AACGGCACTC AAAAGAAAAG GTTCTCCTAA AGGAAAATCA 
TCAGGAGGAA AAGTACCAGT AAAGAAATCA CCTGCTGACT CTACAGACAC ATCACCTGTT 
GCAATAGTGC CGCAGCCACC TAAGCCAGGA TCAGAAGAAT GGGTCTATGT GAATGAACCA 
GTTCCTGAGG AAATGCCTTC GTTTTTAGTA CCTTACTGGG AACTAATAGA AAATTCCTAT 
ATAAACACCA TCAAAACAGT ACTCAGGCAT CTGAGGGAAG ACCAGCATAC TGTGCTTGCT 
TACTTATATG AGATTAGAAC AAGTTTCCAG GAGTTTCTAA AGCGTCCAGA TCACAAGCAA 
GATTTTGTAG CTCAATGGCA GGCTGATTTC AATTCCCTTC CTGATGACCT GTGGGATGAT 
GAGGAAACAA AGGCTGAACT ACACCAACGA GTGAATGATC TGCGAGACCG CCTGTGGGAC 
ATTTGTGACG CACGGAAGGA AGAGGCAGAG CAGGAGCGGC TTGACATCAT TAATGAGAAC 
TGGTTACAGG ACTCTCTTGG AATGACAATG AACCATTTCT TTTCCCTGAT GCAGGCAGAA 
CTGAACCGTT TCCAAGATAC GAAGAGACTC CTTCAAGATT ATTACCGGGG AATGGAAAGT 
AAAATCCCAA TAGAGGACAA TAAGAGATTT ACTCAAATCC CTTTGGTCCA GCTGGATAAT 
AAAGACAATT CTGAAAGCCA GCTTAGAATT CCTCTATTTC CTCGAATATC CATTTCTCTG 
GAAACAGTTA CGCCCAAACC AAAAACAAAA TCAATACTGA AGGGCAAGAT GGATAACTCC 
CTAGAAAACG TTGAGTCCAA CTTTGAGGCT GATGAAAAGT TGGTCATGGA CACCTGGCAA 
CAGGCTTCTT TAGCAGTATC TCACATGGTG GCTGCAGAAA TTCATCAGAG GCTCATGGAA 
GAAGAAAAAG AAAACCAGCC AGCAGACCCC AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT 
AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA AAACAGGAAG ACAAAAAACT CAAAGGTAAA 
TCACCACCTA TTGCAGAAGC AACTCCTGTC ATAGTAACAA CAGAGGAAAT TGCTGAAATC 
GAAAGGAAAA ATGAACTGAG GCTCAAAATA AAAGAAGAAC ACCTTGCTGC CTTGCAATTT 
GAAGAAACAG CCACACAGTT TCGACTTGAA CTGATAAAGA CAAAAGCATT GGCTCTTCTT 
GAAGATTTAG TAACAAAGGT GGTTGATGTA TATAAACTCA TGGAAAAATG GCTTGGTGAG 
AGATATTTGA ATGAAATGGC CAGTGTAGAA AAATTAACTG ACATAGCTCG CTATCACATT 
GAAACATCTA CAAAAATTCA GAATGAACTT TATTTAAACC AAGAAGACTT CTTCATTAAT 
GGCAATGTAA AAGTCTTTCC AGATCCTCCC CCACCGATAC ATCCTCCACC TGTAGAAAAG 
GAAGAAGATG GCACCCTGAC CATCGAACAG CTTGACAATC TTCGGGATCA GTTCTTAGAT 
ATGGCACCTA AAGGCATAAT AGGAAATAAA GCATTTACTG ACATTCTGAT CGATTTGGTG 
ACCCTGAACC TTGGCACAAA CAACTTTCCT AGTAGTTGGA TGCACCTTAC CCAACCTGAA 
TTACAGGAAT TAACATCTTT ATTAACAGTC AACTCTGAGT TTGTGGACTG GCGGAAGTTC 
CTGTTAGTAA CCTCAATGCC TTGGCCCATT CCCTTGGAGG AGGAACTCCT TGAGACCCTT 
CAGAAATTCA AGGCTGTGGA TAAGGCGCAG TTGGGCACCA TCACTTTCGA GCAGTATATG 
CAGGCTGGTC TGTGGTTTAC AGGAGATGAA GATATAAAAA TTCCAGACAA TCCTCTTGAA 
CCCCTGCCAT TTAATAGGCA GGAGCATCTT ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC 
TATGAGAAGG ATCCACCCCA ACTTGACTAC ACACAGATGC TGCTTTACTT TGCTTGCCAC 
CCAGACACCG TGGAAGGAGT CTACAGGGCC CTCAGTGTGG CTGTTGGGAC TCATGTCTTC 
CAACAAGTTG AAGCTTCCAC TCCAAATGCA GAAAAGACCT CCTCAACTGA TGCAAGTCCA 
GCTGAGGAAT TCCCTGAACC TGAGGAAAAT GCTGCAAAAG AAGAAAGGAA ATTAAAAGAC 
AACAGGGAGG AAAGGGAACA GAAGGATGAA GAAATCCCTG AAAATGCAAA CAATGAAAAG 
ATATCCATGG AAACATTACT CAAAGTGTTC AAAGGGGGAA GTGAAGCACA GGACTCTAAC 
AGATTTGCCA GCCACCTAAA GATAGAGAAC ATTTATGCAG AGGGCTTCAT AAAAACATTT 
CAAGACCTAG GTGCCAAGAA CCTGGAGCCA ATTGAAGTTG CTGTTCTCTT GAAGCATCCT 
TTTATTCAAG ACCTGATTTC AAATTATTCA GACTATAAGT TTCCTGTGGA TGGACCAGTG 
AGCTGGCATC AAATCTCTTC AATGCACAAG TCTTATCTTT GGAAAAAAAG AAGGACTTTC 
ACTGGTGCAG CACTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007959546.1
CDS	107..5584
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGFLLGEVLYKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQPLTNLRLQHMKSETFRERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIKKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVANEIKKFEALIKKDLQAKESASKTTLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYKKHYSICAEILDQIVDLSTKVADYRTLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLIELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPMRILSIDTLVQEAIQAFHDNEKVSEILPIQKKDEEDALPVLQEEIKESQASDPQNVFSAGPVSNEGLPETEGETILSANADKTPKAEEIKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINEIPVNQHWILDGFPMTLNQAQLLEEALTGYNRNLTELERKKAQKSTLAIDPATSKEVPLPSPAFDFVVLLDVSDTSSMSRMKDIIAEELSYKTAHEDISQRVAAENQDKGGDQNLRDQIQHRIIGFLDNWPLLKQWFSEPENILIKINAEIDKESLCEKVKEILTTEIVKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHLHHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINENWLQDSLGMTMNHFFSLMQAELNRFQDTKRLLQDYYRGMESKIPIEDNKRFTQIPLVQLDNKDNSESQLRIPLFPRISISLETVTPKPKTKSILKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKLKGKSPPIAEATPVIVTTEEIAEIERKNELRLKIKEEHLAALQFEETATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASVEKLTDIARYHIETSTKIQNELYLNQEDFFINGNVKVFPDPPPPIHPPPVEKEEDGTLTIEQLDNLRDQFLDMAPKGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKAQLGTITFEQYMQAGLWFTGDEDIKIPDNPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVEASTPNAEKTSSTDASPAEEFPEPEENAAKEERKLKDNREEREQKDEEIPENANNEKISMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVDGPVSWHQISSMHKSYLWKKRRTFTGAAL

Target ORF information:

RefSeq Version	XM_007961355.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007961355.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG CAAGTGATCC ACAAAATGTA 
TTTTCAGCTG GTCCAGTTTC AAATGAAGGA TTACCAGAAA CAGAAGGTGA AACGATACTA 
AGTGCTAATG CTGATAAAAC ACCAAAAGCT GAAGAAATCA AATCCAGTGA CAGTTTCTTA 
AAACTCACTA CGCGTGCTCA ACTTGGTGCA AAATCAGAAC AGTTGCTGAA GAAAGGAAAG 
AGCATTCCTG ACGTGCTGCT TGTTGACATC ATGGTAAATG CTATTAACGA GATACCTGTG 
AATCAGCACT GGATCCTAGA TGGTTTTCCA ATGACTTTAA ATCAAGCACA GCTTCTGGAA 
GAAGCTCTTA CAGGCTACAA TAGAAACCTC ACAGAACTGG AGAGAAAAAA AGCACAAAAA 
TCCACATTGG CTATTGATCC TGCGACTTCC AAAGAAGTAC CTCTTCCCTC TCCTGCATTT 
GATTTTGTCG TATTATTAGA CGTTTCAGAT ACTTCCTCAA TGAGTCGCAT GAAGGATATA 
ATAGCTGAAG AATTGTCCTA TAAAACTGCT CACGAAGACA TCAGTCAACG TGTAGCTGCT 
GAAAATCAAG ATAAGGGTGG AGACCAAAAT TTAAGAGACC AGATACAACA TAGAATTATA 
GGCTTCTTGG ACAACTGGCC TTTATTGAAG CAATGGTTTT CAGAGCCAGA AAATATTTTG 
ATAAAAATCA ATGCTGAAAT AGATAAGGAA TCTTTATGCG AAAAAGTAAA AGAAATTCTT 
ACGACTGAAA TAGTGAAAAA AAAGAATAAA GTTGAGAAGA AATTAGAAGA AAAGGAAGCT 
GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG CTTCCACTTC CTACACCTCC TCCTGCTCCT 
CCTCCTGAAC CAGAAAAAGA GAAGGAAATT CATCTTCATC ATGTGCCTTC AAAAACTCCT 
ACTGCAAAAG GAAAACCTCA ATCAGAAGCC CCGCATGGTA AGCAAGAATC TCTTCAGGAA 
GGAAAAGGGA AGAAAGGTGA AACGGCACTC AAAAGAAAAG GTTCTCCTAA AGGAAAATCA 
TCAGGAGGAA AAGTACCAGT AAAGAAATCA CCTGCTGACT CTACAGACAC ATCACCTGTT 
GCAATAGTGC CGCAGCCACC TAAGCCAGGA TCAGAAGAAT GGGTCTATGT GAATGAACCA 
GTTCCTGAGG AAATGCCTTC GTTTTTAGTA CCTTACTGGG AACTAATAGA AAATTCCTAT 
ATAAACACCA TCAAAACAGT ACTCAGGCAT CTGAGGGAAG ACCAGCATAC TGTGCTTGCT 
TACTTATATG AGATTAGAAC AAGTTTCCAG GAGTTTCTAA AGCGTCCAGA TCACAAGCAA 
GATTTTGTAG CTCAATGGCA GGCTGATTTC AATTCCCTTC CTGATGACCT GTGGGATGAT 
GAGGAAACAA AGGCTGAACT ACACCAACGA GTGAATGATC TGCGAGACCG CCTGTGGGAC 
ATTTGTGACG CACGGAAGGA AGAGGCAGAG CAGGAGCGGC TTGACATCAT TAATGAGAAC 
TGGTTACAGG ACTCTCTTGG AATGACAATG AACCATTTCT TTTCCCTGAT GCAGGCAGAA 
CTGAACCGTT TCCAAGATAC GAAGAGACTC CTTCAAGATT ATTACCGGGG AATGGAAAGT 
AAAATCCCAA TAGAGGACAA TAAGAGATTT ACTCAAATCC CTTTGGTCCA GCTGGATAAT 
AAAGACAATT CTGAAAGCCA GCTTAGAATT CCTCTATTTC CTCGAATATC CATTTCTCTG 
GAAACAGTTA CGCCCAAACC AAAAACAAAA TCAATACTGA AGGGCAAGAT GGATAACTCC 
CTAGAAAACG TTGAGTCCAA CTTTGAGGCT GATGAAAAGT TGGTCATGGA CACCTGGCAA 
CAGGCTTCTT TAGCAGTATC TCACATGGTG GCTGCAGAAA TTCATCAGAG GCTCATGGAA 
GAAGAAAAAG AAAACCAGCC AGCAGACCCC AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT 
AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA AAACAGGAAG ACAAAAAACT CAAAGGTAAA 
TCACCACCTA TTGCAGAAGC AACTCCTGTC ATAGTAACAA CAGAGGAAAT TGCTGAAATC 
GAAAGGAAAA ATGAACTGAG GCTCAAAATA AAAGAAGAAC ACCTTGCTGC CTTGCAATTT 
GAAGAAACAG CCACACAGTT TCGACTTGAA CTGATAAAGA CAAAAGCATT GGCTCTTCTT 
GAAGATTTAG TAACAAAGGT GGTTGATGTA TATAAACTCA TGGAAAAATG GCTTGGTGAG 
AGATATTTGA ATGAAATGGC CAGTGTAGAA AAATTAACTG ACATAGCTCG CTATCACATT 
GAAACATCTA CAAAAATTCA GAATGAACTT TATTTAAACC AAGAAGACTT CTTCATTAAT 
GGCAATGTAA AAGTCTTTCC AGATCCTCCC CCACCGATAC ATCCTCCACC TGTAGAAAAG 
GAAGAAGATG GCACCCTGAC CATCGAACAG CTTGACAATC TTCGGGATCA GTTCTTAGAT 
ATGGCACCTA AAGGCATAAT AGGAAATAAA GCATTTACTG ACATTCTGAT CGATTTGGTG 
ACCCTGAACC TTGGCACAAA CAACTTTCCT AGTAGTTGGA TGCACCTTAC CCAACCTGAA 
TTACAGGAAT TAACATCTTT ATTAACAGTC AACTCTGAGT TTGTGGACTG GCGGAAGTTC 
CTGTTAGTAA CCTCAATGCC TTGGCCCATT CCCTTGGAGG AGGAACTCCT TGAGACCCTT 
CAGAAATTCA AGGCTGTGGA TAAGGCGCAG TTGGGCACCA TCACTTTCGA GCAGTATATG 
CAGGCTGGTC TGTGGTTTAC AGGAGATGAA GATATAAAAA TTCCAGACAA TCCTCTTGAA 
CCCCTGCCAT TTAATAGGCA GGAGCATCTT ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC 
TATGAGAAGG ATCCACCCCA ACTTGACTAC ACACAGATGC TGCTTTACTT TGCTTGCCAC 
CCAGACACCG TGGAAGGAGT CTACAGGGCC CTCAGTGTGG CTGTTGGGAC TCATGTCTTC 
CAACAAGTTG AAGCTTCCAC TCCAAATGCA GAAAAGACCT CCTCAACTGA TGCAAGTCCA 
GCTGAGGAAT TCCCTGAACC TGAGGAAAAT GCTGCAAAAG AAGAAAGGAA ATTAAAAGAC 
AACAGGGAGG AAAGGGAACA GAAGGATGAA GAAATCCCTG AAAATGCAAA CAATGAAAAG 
ATATCCATGG AAACATTACT CAAAGTGTTC AAAGGGGGAA GTGAAGCACA GGACTCTAAC 
AGATTTGCCA GCCACCTAAA GATAGAGAAC ATTTATGCAG AGGGCTTCAT AAAAACATTT 
CAAGACCTAG GTGCCAAGAA CCTGGAGCCA ATTGAAGTTG CTGTTCTCTT GAAGCATCCT 
TTTATTCAAG ACCTGATTTC AAATTATTCA GACTATAAGT TTCCTGTGGA TGGACCAGTG 
AGCTGGCATC AAATCTCTTC AATGCACAAG TCTTATCTTT GGAAAAAAAG AAGGACTTTC 
ACTGGTGCAG CACTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_007961356.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007959547.1 Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X10, mRNA.

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CloneID	OCh83466
Clone ID Related Accession (Same CDS sequence)	XM_007961356.1
Accession Version	XM_007961356.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	sperm flagellar protein 2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735239.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG CAAGTGATCC ACAAAATGTA 
TTTTCAGCTG GTCCAGTTTC AAATGAAGGA TTACCAGAAA CAGAAGGTGA AACGATACTA 
AGTGCTAATG CTGATAAAAC ACCAAAAGCT GAAGAAATCA AATCCAGTGA CAGTTTCTTA 
AAACTCACTA CGCGTGCTCA ACTTGGTGCA AAATCAGAAC AGTTGCTGAA GAAAGGAAAG 
AGCATTCCTG ACGTGCTGCT TGTTGACATC ATGGTAAATG CTATTAACGA GATACCTGTG 
AATCAGCACT GGATCCTAGA TGGTTTTCCA ATGACTTTAA ATCAAGCACA GCTTCTGGAA 
GAAGCTCTTA CAGGCTACAA TAGAAACCTC ACAGAACTGG AGAGAAAAAA AGCACAAAAA 
TCCACATTGG CTATTGATCC TGCGACTTCC AAAGAAGTAC CTCTTCCCTC TCCTGCATTT 
GATTTTGTCG TATTATTAGA CGTTTCAGAT ACTTCCTCAA TGAGTCGCAT GAAGGATATA 
ATAGCTGAAG AATTGTCCTA TAAAACTGCT CACGAAGACA TCAGTCAACG TGTAGCTGCT 
GAAAATCAAG ATAAGGGTGG AGACCAAAAT TTAAGAGACC AGATACAACA TAGAATTATA 
GGCTTCTTGG ACAACTGGCC TTTATTGAAG CAATGGTTTT CAGAGCCAGA AAATATTTTG 
ATAAAAATCA ATGCTGAAAT AGATAAGGAA TCTTTATGCG AAAAAGTAAA AGAAATTCTT 
ACGACTGAAA TAGTGAAAAA AAAGAATAAA GTTGAGAAGA AATTAGAAGA AAAGGAAGCT 
GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG CTTCCACTTC CTACACCTCC TCCTGCTCCT 
CCTCCTGAAC CAGAAAAAGA GAAGGAAATT CATCTTCATC ATGTGCCTTC AAAAACTCCT 
ACTGCAAAAG GAAAACCTCA ATCAGAAGCC CCGCATGGTA AGCAAGAATC TCTTCAGGAA 
GGAAAAGGGA AGAAAGGTGA AACGGCACTC AAAAGAAAAG GTTCTCCTAA AGGAAAATCA 
TCAGGAGGAA AAGTACCAGT AAAGAAATCA CCTGCTGACT CTACAGACAC ATCACCTGTT 
GCAATAGTGC CGCAGCCACC TAAGCCAGGA TCAGAAGAAT GGGTCTATGT GAATGAACCA 
GTTCCTGAGG AAATGCCTTC GTTTTTAGTA CCTTACTGGG AACTAATAGA AAATTCCTAT 
ATAAACACCA TCAAAACAGT ACTCAGGCAT CTGAGGGAAG ACCAGCATAC TGTGCTTGCT 
TACTTATATG AGATTAGAAC AAGTTTCCAG GAGTTTCTAA AGCGTCCAGA TCACAAGCAA 
GATTTTGTAG CTCAATGGCA GGCTGATTTC AATTCCCTTC CTGATGACCT GTGGGATGAT 
GAGGAAACAA AGGCTGAACT ACACCAACGA GTGAATGATC TGCGAGACCG CCTGTGGGAC 
ATTTGTGACG CACGGAAGGA AGAGGCAGAG CAGGAGCGGC TTGACATCAT TAATGAGAAC 
TGGTTACAGG ACTCTCTTGG AATGACAATG AACCATTTCT TTTCCCTGAT GCAGGCAGAA 
CTGAACCGTT TCCAAGATAC GAAGAGACTC CTTCAAGATT ATTACCGGGG AATGGAAAGT 
AAAATCCCAA TAGAGGACAA TAAGAGATTT ACTCAAATCC CTTTGGTCCA GCTGGATAAT 
AAAGACAATT CTGAAAGCCA GCTTAGAATT CCTCTATTTC CTCGAATATC CATTTCTCTG 
GAAACAGTTA CGCCCAAACC AAAAACAAAA TCAATACTGA AGGGCAAGAT GGATAACTCC 
CTAGAAAACG TTGAGTCCAA CTTTGAGGCT GATGAAAAGT TGGTCATGGA CACCTGGCAA 
CAGGCTTCTT TAGCAGTATC TCACATGGTG GCTGCAGAAA TTCATCAGAG GCTCATGGAA 
GAAGAAAAAG AAAACCAGCC AGCAGACCCC AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT 
AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA AAACAGGAAG ACAAAAAACT CAAAGGTAAA 
TCACCACCTA TTGCAGAAGC AACTCCTGTC ATAGTAACAA CAGAGGAAAT TGCTGAAATC 
GAAAGGAAAA ATGAACTGAG GCTCAAAATA AAAGAAGAAC ACCTTGCTGC CTTGCAATTT 
GAAGAAACAG CCACACAGTT TCGACTTGAA CTGATAAAGA CAAAAGCATT GGCTCTTCTT 
GAAGATTTAG TAACAAAGGT GGTTGATGTA TATAAACTCA TGGAAAAATG GCTTGGTGAG 
AGATATTTGA ATGAAATGGC CAGTGTAGAA AAATTAACTG ACATAGCTCG CTATCACATT 
GAAACATCTA CAAAAATTCA GAATGAACTT TATTTAAACC AAGAAGACTT CTTCATTAAT 
GGCAATGTAA AAGTCTTTCC AGATCCTCCC CCACCGATAC ATCCTCCACC TGTAGAAAAG 
GAAGAAGATG GCACCCTGAC CATCGAACAG CTTGACAATC TTCGGGATCA GTTCTTAGAT 
ATGGCACCTA AAGGCATAAT AGGAAATAAA GCATTTACTG ACATTCTGAT CGATTTGGTG 
ACCCTGAACC TTGGCACAAA CAACTTTCCT AGTAGTTGGA TGCACCTTAC CCAACCTGAA 
TTACAGGAAT TAACATCTTT ATTAACAGTC AACTCTGAGT TTGTGGACTG GCGGAAGTTC 
CTGTTAGTAA CCTCAATGCC TTGGCCCATT CCCTTGGAGG AGGAACTCCT TGAGACCCTT 
CAGAAATTCA AGGCTGTGGA TAAGGCGCAG TTGGGCACCA TCACTTTCGA GCAGTATATG 
CAGGCTGGTC TGTGGTTTAC AGGAGATGAA GATATAAAAA TTCCAGACAA TCCTCTTGAA 
CCCCTGCCAT TTAATAGGCA GGAGCATCTT ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC 
TATGAGAAGG ATCCACCCCA ACTTGACTAC ACACAGATGC TGCTTTACTT TGCTTGCCAC 
CCAGACACCG TGGAAGGAGT CTACAGGGCC CTCAGTGTGG CTGTTGGGAC TCATGTCTTC 
CAACAAGTTG AAGCTTCCAC TCCAAATGCA GAAAAGACCT CCTCAACTGA TGCAAGTCCA 
GCTGAGGAAT TCCCTGAACC TGAGGAAAAT GCTGCAAAAG AAGAAAGGAA ATTAAAAGAC 
AACAGGGAGG AAAGGGAACA GAAGGATGAA GAAATCCCTG AAAATGCAAA CAATGAAAAG 
ATATCCATGG AAACATTACT CAAAGTGTTC AAAGGGGGAA GTGAAGCACA GGACTCTAAC 
AGATTTGCCA GCCACCTAAA GATAGAGAAC ATTTATGCAG AGGGCTTCAT AAAAACATTT 
CAAGACCTAG GTGCCAAGAA CCTGGAGCCA ATTGAAGTTG CTGTTCTCTT GAAGCATCCT 
TTTATTCAAG ACCTGATTTC AAATTATTCA GACTATAAGT TTCCTGATAT TAAAATAATT 
CTCCAAAGGA GTGAACATGT ACCAGGAAGT GATGGAGAGA GATCACCTTT AAGACATACA 
GAGGAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007959547.1
CDS	107..5581
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGFLLGEVLYKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQPLTNLRLQHMKSETFRERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIKKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVANEIKKFEALIKKDLQAKESASKTTLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYKKHYSICAEILDQIVDLSTKVADYRTLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLIELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPMRILSIDTLVQEAIQAFHDNEKVSEILPIQKKDEEDALPVLQEEIKESQASDPQNVFSAGPVSNEGLPETEGETILSANADKTPKAEEIKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINEIPVNQHWILDGFPMTLNQAQLLEEALTGYNRNLTELERKKAQKSTLAIDPATSKEVPLPSPAFDFVVLLDVSDTSSMSRMKDIIAEELSYKTAHEDISQRVAAENQDKGGDQNLRDQIQHRIIGFLDNWPLLKQWFSEPENILIKINAEIDKESLCEKVKEILTTEIVKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHLHHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINENWLQDSLGMTMNHFFSLMQAELNRFQDTKRLLQDYYRGMESKIPIEDNKRFTQIPLVQLDNKDNSESQLRIPLFPRISISLETVTPKPKTKSILKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKLKGKSPPIAEATPVIVTTEEIAEIERKNELRLKIKEEHLAALQFEETATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASVEKLTDIARYHIETSTKIQNELYLNQEDFFINGNVKVFPDPPPPIHPPPVEKEEDGTLTIEQLDNLRDQFLDMAPKGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKAQLGTITFEQYMQAGLWFTGDEDIKIPDNPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVEASTPNAEKTSSTDASPAEEFPEPEENAAKEERKLKDNREEREQKDEEIPENANNEKISMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPDIKIILQRSEHVPGSDGERSPLRHTEEKK

Target ORF information:

RefSeq Version	XM_007961356.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007961356.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG CAAGTGATCC ACAAAATGTA 
TTTTCAGCTG GTCCAGTTTC AAATGAAGGA TTACCAGAAA CAGAAGGTGA AACGATACTA 
AGTGCTAATG CTGATAAAAC ACCAAAAGCT GAAGAAATCA AATCCAGTGA CAGTTTCTTA 
AAACTCACTA CGCGTGCTCA ACTTGGTGCA AAATCAGAAC AGTTGCTGAA GAAAGGAAAG 
AGCATTCCTG ACGTGCTGCT TGTTGACATC ATGGTAAATG CTATTAACGA GATACCTGTG 
AATCAGCACT GGATCCTAGA TGGTTTTCCA ATGACTTTAA ATCAAGCACA GCTTCTGGAA 
GAAGCTCTTA CAGGCTACAA TAGAAACCTC ACAGAACTGG AGAGAAAAAA AGCACAAAAA 
TCCACATTGG CTATTGATCC TGCGACTTCC AAAGAAGTAC CTCTTCCCTC TCCTGCATTT 
GATTTTGTCG TATTATTAGA CGTTTCAGAT ACTTCCTCAA TGAGTCGCAT GAAGGATATA 
ATAGCTGAAG AATTGTCCTA TAAAACTGCT CACGAAGACA TCAGTCAACG TGTAGCTGCT 
GAAAATCAAG ATAAGGGTGG AGACCAAAAT TTAAGAGACC AGATACAACA TAGAATTATA 
GGCTTCTTGG ACAACTGGCC TTTATTGAAG CAATGGTTTT CAGAGCCAGA AAATATTTTG 
ATAAAAATCA ATGCTGAAAT AGATAAGGAA TCTTTATGCG AAAAAGTAAA AGAAATTCTT 
ACGACTGAAA TAGTGAAAAA AAAGAATAAA GTTGAGAAGA AATTAGAAGA AAAGGAAGCT 
GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG CTTCCACTTC CTACACCTCC TCCTGCTCCT 
CCTCCTGAAC CAGAAAAAGA GAAGGAAATT CATCTTCATC ATGTGCCTTC AAAAACTCCT 
ACTGCAAAAG GAAAACCTCA ATCAGAAGCC CCGCATGGTA AGCAAGAATC TCTTCAGGAA 
GGAAAAGGGA AGAAAGGTGA AACGGCACTC AAAAGAAAAG GTTCTCCTAA AGGAAAATCA 
TCAGGAGGAA AAGTACCAGT AAAGAAATCA CCTGCTGACT CTACAGACAC ATCACCTGTT 
GCAATAGTGC CGCAGCCACC TAAGCCAGGA TCAGAAGAAT GGGTCTATGT GAATGAACCA 
GTTCCTGAGG AAATGCCTTC GTTTTTAGTA CCTTACTGGG AACTAATAGA AAATTCCTAT 
ATAAACACCA TCAAAACAGT ACTCAGGCAT CTGAGGGAAG ACCAGCATAC TGTGCTTGCT 
TACTTATATG AGATTAGAAC AAGTTTCCAG GAGTTTCTAA AGCGTCCAGA TCACAAGCAA 
GATTTTGTAG CTCAATGGCA GGCTGATTTC AATTCCCTTC CTGATGACCT GTGGGATGAT 
GAGGAAACAA AGGCTGAACT ACACCAACGA GTGAATGATC TGCGAGACCG CCTGTGGGAC 
ATTTGTGACG CACGGAAGGA AGAGGCAGAG CAGGAGCGGC TTGACATCAT TAATGAGAAC 
TGGTTACAGG ACTCTCTTGG AATGACAATG AACCATTTCT TTTCCCTGAT GCAGGCAGAA 
CTGAACCGTT TCCAAGATAC GAAGAGACTC CTTCAAGATT ATTACCGGGG AATGGAAAGT 
AAAATCCCAA TAGAGGACAA TAAGAGATTT ACTCAAATCC CTTTGGTCCA GCTGGATAAT 
AAAGACAATT CTGAAAGCCA GCTTAGAATT CCTCTATTTC CTCGAATATC CATTTCTCTG 
GAAACAGTTA CGCCCAAACC AAAAACAAAA TCAATACTGA AGGGCAAGAT GGATAACTCC 
CTAGAAAACG TTGAGTCCAA CTTTGAGGCT GATGAAAAGT TGGTCATGGA CACCTGGCAA 
CAGGCTTCTT TAGCAGTATC TCACATGGTG GCTGCAGAAA TTCATCAGAG GCTCATGGAA 
GAAGAAAAAG AAAACCAGCC AGCAGACCCC AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT 
AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA AAACAGGAAG ACAAAAAACT CAAAGGTAAA 
TCACCACCTA TTGCAGAAGC AACTCCTGTC ATAGTAACAA CAGAGGAAAT TGCTGAAATC 
GAAAGGAAAA ATGAACTGAG GCTCAAAATA AAAGAAGAAC ACCTTGCTGC CTTGCAATTT 
GAAGAAACAG CCACACAGTT TCGACTTGAA CTGATAAAGA CAAAAGCATT GGCTCTTCTT 
GAAGATTTAG TAACAAAGGT GGTTGATGTA TATAAACTCA TGGAAAAATG GCTTGGTGAG 
AGATATTTGA ATGAAATGGC CAGTGTAGAA AAATTAACTG ACATAGCTCG CTATCACATT 
GAAACATCTA CAAAAATTCA GAATGAACTT TATTTAAACC AAGAAGACTT CTTCATTAAT 
GGCAATGTAA AAGTCTTTCC AGATCCTCCC CCACCGATAC ATCCTCCACC TGTAGAAAAG 
GAAGAAGATG GCACCCTGAC CATCGAACAG CTTGACAATC TTCGGGATCA GTTCTTAGAT 
ATGGCACCTA AAGGCATAAT AGGAAATAAA GCATTTACTG ACATTCTGAT CGATTTGGTG 
ACCCTGAACC TTGGCACAAA CAACTTTCCT AGTAGTTGGA TGCACCTTAC CCAACCTGAA 
TTACAGGAAT TAACATCTTT ATTAACAGTC AACTCTGAGT TTGTGGACTG GCGGAAGTTC 
CTGTTAGTAA CCTCAATGCC TTGGCCCATT CCCTTGGAGG AGGAACTCCT TGAGACCCTT 
CAGAAATTCA AGGCTGTGGA TAAGGCGCAG TTGGGCACCA TCACTTTCGA GCAGTATATG 
CAGGCTGGTC TGTGGTTTAC AGGAGATGAA GATATAAAAA TTCCAGACAA TCCTCTTGAA 
CCCCTGCCAT TTAATAGGCA GGAGCATCTT ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC 
TATGAGAAGG ATCCACCCCA ACTTGACTAC ACACAGATGC TGCTTTACTT TGCTTGCCAC 
CCAGACACCG TGGAAGGAGT CTACAGGGCC CTCAGTGTGG CTGTTGGGAC TCATGTCTTC 
CAACAAGTTG AAGCTTCCAC TCCAAATGCA GAAAAGACCT CCTCAACTGA TGCAAGTCCA 
GCTGAGGAAT TCCCTGAACC TGAGGAAAAT GCTGCAAAAG AAGAAAGGAA ATTAAAAGAC 
AACAGGGAGG AAAGGGAACA GAAGGATGAA GAAATCCCTG AAAATGCAAA CAATGAAAAG 
ATATCCATGG AAACATTACT CAAAGTGTTC AAAGGGGGAA GTGAAGCACA GGACTCTAAC 
AGATTTGCCA GCCACCTAAA GATAGAGAAC ATTTATGCAG AGGGCTTCAT AAAAACATTT 
CAAGACCTAG GTGCCAAGAA CCTGGAGCCA ATTGAAGTTG CTGTTCTCTT GAAGCATCCT 
TTTATTCAAG ACCTGATTTC AAATTATTCA GACTATAAGT TTCCTGATAT TAAAATAATT 
CTCCAAAGGA GTGAACATGT ACCAGGAAGT GATGGAGAGA GATCACCTTT AAGACATACA 
GAGGAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_007961357.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007959548.1 Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X11, mRNA.

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CloneID	OCh83467
Clone ID Related Accession (Same CDS sequence)	XM_007961357.1
Accession Version	XM_007961357.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	sperm flagellar protein 2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735239.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG CAAGTGATCC ACAAAATGTA 
TTTTCAGCTG GTCCAGTTTC AAATGAAGGA TTACCAGAAA CAGAAGGTGA AACGATACTA 
AGTGCTAATG CTGATAAAAC ACCAAAAGCT GAAGAAATCA AATCCAGTGA CAGTTTCTTA 
AAACTCACTA CGCGTGCTCA ACTTGGTGCA AAATCAGAAC AGTTGCTGAA GAAAGGAAAG 
AGCATTCCTG ACGTGCTGCT TGTTGACATC ATGGTAAATG CTATTAACGA GATACCTGTG 
AATCAGCACT GGATCCTAGA TGGTTTTCCA ATGACTTTAA ATCAAGCACA GCTTCTGGAA 
GAAGCTCTTA CAGGCTACAA TAGAAACCTC ACAGAACTGG AGAGAAAAAA AGCACAAAAA 
TCCACATTGG CTATTGATCC TGCGACTTCC AAAGAAGTAC CTCTTCCCTC TCCTGCATTT 
GATTTTGTCG TATTATTAGA CGTTTCAGAT ACTTCCTCAA TGAGTCGCAT GAAGGATATA 
ATAGCTGAAG AATTGTCCTA TAAAACTGCT CACGAAGACA TCAGTCAACG TGTAGCTGCT 
GAAAATCAAG ATAAGGGTGG AGACCAAAAT TTAAGAGACC AGATACAACA TAGAATTATA 
GGCTTCTTGG ACAACTGGCC TTTATTGAAG CAATGGTTTT CAGAGCCAGA AAATATTTTG 
ATAAAAATCA ATGCTGAAAT AGATAAGGAA TCTTTATGCG AAAAAGTAAA AGAAATTCTT 
ACGACTGAAA TAGTGAAAAA AAAGAATAAA GTTGAGAAGA AATTAGAAGA AAAGGAAGCT 
GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG CTTCCACTTC CTACACCTCC TCCTGCTCCT 
CCTCCTGAAC CAGAAAAAGA GAAGGAAATT CATCTTCATC ATGTGCCTTC AAAAACTCCT 
ACTGCAAAAG GAAAACCTCA ATCAGAAGCC CCGCATGGTA AGCAAGAATC TCTTCAGGAA 
GGAAAAGGGA AGAAAGGTGA AACGGCACTC AAAAGAAAAG GTTCTCCTAA AGGAAAATCA 
TCAGGAGGAA AAGTACCAGT AAAGAAATCA CCTGCTGACT CTACAGACAC ATCACCTGTT 
GCAATAGTGC CGCAGCCACC TAAGCCAGGA TCAGAAGAAT GGGTCTATGT GAATGAACCA 
GTTCCTGAGG AAATGCCTTC GTTTTTAGTA CCTTACTGGG AACTAATAGA AAATTCCTAT 
ATAAACACCA TCAAAACAGT ACTCAGGCAT CTGAGGGAAG ACCAGCATAC TGTGCTTGCT 
TACTTATATG AGATTAGAAC AAGTTTCCAG GAGTTTCTAA AGCGTCCAGA TCACAAGCAA 
GATTTTGTAG CTCAATGGCA GGCTGATTTC AATTCCCTTC CTGATGACCT GTGGGATGAT 
GAGGAAACAA AGGCTGAACT ACACCAACGA GTGAATGATC TGCGAGACCG CCTGTGGGAC 
ATTTGTGACG CACGGAAGGA AGAGGCAGAG CAGGAGCGGC TTGACATCAT TAATGAGAAC 
TGGTTACAGG ACTCTCTTGG AATGACAATG AACCATTTCT TTTCCCTGAT GCAGGCAGAA 
CTGAACCGTT TCCAAGATAC GAAGAGACTC CTTCAAGATT ATTACCGGGG AATGGAAAGT 
AAAATCCCAA TAGAGGACAA TAAGAGATTT ACTCAAATCC CTTTGGTCCA GCTGGATAAT 
AAAGACAATT CTGAAAGCCA GCTTAGAATT CCTCTATTTC CTCGAATATC CATTTCTCTG 
GAAACAGTTA CGCCCAAACC AAAAACAAAA TCAATACTGA AGGGCAAGAT GGATAACTCC 
CTAGAAAACG TTGAGTCCAA CTTTGAGGCT GATGAAAAGT TGGTCATGGA CACCTGGCAA 
CAGGCTTCTT TAGCAGTATC TCACATGGTG GCTGCAGAAA TTCATCAGAG GCTCATGGAA 
GAAGAAAAAG AAAACCAGCC AGCAGACCCC AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT 
AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA AAACAGGAAG ACAAAAAACT CAAAGGTAAA 
TCACCACCTA TTGCAGAAGC AACTCCTGTC ATAGTAACAA CAGAGGAAAT TGCTGAAATC 
GAAAGGAAAA ATGAACTGAG GCTCAAAATA AAAGAAGAAC ACCTTGCTGC CTTGCAATTT 
GAAGAAACAG CCACACAGTT TCGACTTGAA CTGATAAAGA CAAAAGCATT GGCTCTTCTT 
GAAGATTTAG TAACAAAGGT GGTTGATGTA TATAAACTCA TGGAAAAATG GCTTGGTGAG 
AGATATTTGA ATGAAATGGC CAGTGTAGAA AAATTAACTG ACATAGCTCG CTATCACATT 
GAAACATCTA CAAAAATTCA GAATGAACTT TATTTAAACC AAGAAGACTT CTTCATTAAT 
GGCAATGTAA AAGTCTTTCC AGATCCTCCC CCACCGATAC ATCCTCCACC TGTAGAAAAG 
GAAGAAGATG GCACCCTGAC CATCGAACAG CTTGACAATC TTCGGGATCA GTTCTTAGAT 
ATGGCACCTA AAGGCATAAT AGGAAATAAA GCATTTACTG ACATTCTGAT CGATTTGGTG 
ACCCTGAACC TTGGCACAAA CAACTTTCCT AGTAGTTGGA TGCACCTTAC CCAACCTGAA 
TTACAGGAAT TAACATCTTT ATTAACAGTC AACTCTGAGT TTGTGGACTG GCGGAAGTTC 
CTGTTAGTAA CCTCAATGCC TTGGCCCATT CCCTTGGAGG AGGAACTCCT TGAGACCCTT 
CAGAAATTCA AGGCTGTGGA TAAGGCGCAG TTGGGCACCA TCACTTTCGA GCAGTATATG 
CAGGCTGGTC TGTGGTTTAC AGGAGATGAA GATATAAAAA TTCCAGACAA TCCTCTTGAA 
CCCCTGCCAT TTAATAGGCA GGAGCATCTT ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC 
TATGAGAAGG ATCCACCCCA ACTTGACTAC ACACAGATGC TGCTTTACTT TGCTTGCCAC 
CCAGACACCG TGGAAGGAGT CTACAGGGCC CTCAGTGTGG CTGTTGGGAC TCATGTCTTC 
CAACAAGTTG AAGCTTCCAC TCCAAATGCA GAAAAGACCT CCTCAACTGA TGCAAGTCCA 
GCTGAGGAAT TCCCTGAACC TGAGGAAAAT GCTGCAAAAG AAGAAAGGAA ATTAAAAGAC 
AACAGGGAGG AAAGGGAACA GAAGGATGAA GAAATCCCTG AAAATGCAAA CAATGAAAAG 
ATATCCATGG AAACATTACT CAAAGTGTTC AAAGGGGGAA GTGAAGCACA GGACTCTAAC 
AGATTTGCCA GCCACCTAAA GATAGAGAAC ATTTATGCAG AGGGCTTCAT AAAAACATTT 
CAAGACCTAG GTGCCAAGAA CCTGGAGCCA ATTGAAGTTG CTGTTCTCTT GAAGCATCCT 
TTTATTCAAG ACCTGATTTC AAATTATTCA GACTATAAGT TTCCTTGGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007959548.1
CDS	107..5500
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGFLLGEVLYKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQPLTNLRLQHMKSETFRERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIKKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVANEIKKFEALIKKDLQAKESASKTTLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYKKHYSICAEILDQIVDLSTKVADYRTLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLIELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPMRILSIDTLVQEAIQAFHDNEKVSEILPIQKKDEEDALPVLQEEIKESQASDPQNVFSAGPVSNEGLPETEGETILSANADKTPKAEEIKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINEIPVNQHWILDGFPMTLNQAQLLEEALTGYNRNLTELERKKAQKSTLAIDPATSKEVPLPSPAFDFVVLLDVSDTSSMSRMKDIIAEELSYKTAHEDISQRVAAENQDKGGDQNLRDQIQHRIIGFLDNWPLLKQWFSEPENILIKINAEIDKESLCEKVKEILTTEIVKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHLHHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINENWLQDSLGMTMNHFFSLMQAELNRFQDTKRLLQDYYRGMESKIPIEDNKRFTQIPLVQLDNKDNSESQLRIPLFPRISISLETVTPKPKTKSILKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKLKGKSPPIAEATPVIVTTEEIAEIERKNELRLKIKEEHLAALQFEETATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASVEKLTDIARYHIETSTKIQNELYLNQEDFFINGNVKVFPDPPPPIHPPPVEKEEDGTLTIEQLDNLRDQFLDMAPKGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKAQLGTITFEQYMQAGLWFTGDEDIKIPDNPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVEASTPNAEKTSSTDASPAEEFPEPEENAAKEERKLKDNREEREQKDEEIPENANNEKISMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPWK

Target ORF information:

RefSeq Version	XM_007961357.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007961357.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG CAAGTGATCC ACAAAATGTA 
TTTTCAGCTG GTCCAGTTTC AAATGAAGGA TTACCAGAAA CAGAAGGTGA AACGATACTA 
AGTGCTAATG CTGATAAAAC ACCAAAAGCT GAAGAAATCA AATCCAGTGA CAGTTTCTTA 
AAACTCACTA CGCGTGCTCA ACTTGGTGCA AAATCAGAAC AGTTGCTGAA GAAAGGAAAG 
AGCATTCCTG ACGTGCTGCT TGTTGACATC ATGGTAAATG CTATTAACGA GATACCTGTG 
AATCAGCACT GGATCCTAGA TGGTTTTCCA ATGACTTTAA ATCAAGCACA GCTTCTGGAA 
GAAGCTCTTA CAGGCTACAA TAGAAACCTC ACAGAACTGG AGAGAAAAAA AGCACAAAAA 
TCCACATTGG CTATTGATCC TGCGACTTCC AAAGAAGTAC CTCTTCCCTC TCCTGCATTT 
GATTTTGTCG TATTATTAGA CGTTTCAGAT ACTTCCTCAA TGAGTCGCAT GAAGGATATA 
ATAGCTGAAG AATTGTCCTA TAAAACTGCT CACGAAGACA TCAGTCAACG TGTAGCTGCT 
GAAAATCAAG ATAAGGGTGG AGACCAAAAT TTAAGAGACC AGATACAACA TAGAATTATA 
GGCTTCTTGG ACAACTGGCC TTTATTGAAG CAATGGTTTT CAGAGCCAGA AAATATTTTG 
ATAAAAATCA ATGCTGAAAT AGATAAGGAA TCTTTATGCG AAAAAGTAAA AGAAATTCTT 
ACGACTGAAA TAGTGAAAAA AAAGAATAAA GTTGAGAAGA AATTAGAAGA AAAGGAAGCT 
GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG CTTCCACTTC CTACACCTCC TCCTGCTCCT 
CCTCCTGAAC CAGAAAAAGA GAAGGAAATT CATCTTCATC ATGTGCCTTC AAAAACTCCT 
ACTGCAAAAG GAAAACCTCA ATCAGAAGCC CCGCATGGTA AGCAAGAATC TCTTCAGGAA 
GGAAAAGGGA AGAAAGGTGA AACGGCACTC AAAAGAAAAG GTTCTCCTAA AGGAAAATCA 
TCAGGAGGAA AAGTACCAGT AAAGAAATCA CCTGCTGACT CTACAGACAC ATCACCTGTT 
GCAATAGTGC CGCAGCCACC TAAGCCAGGA TCAGAAGAAT GGGTCTATGT GAATGAACCA 
GTTCCTGAGG AAATGCCTTC GTTTTTAGTA CCTTACTGGG AACTAATAGA AAATTCCTAT 
ATAAACACCA TCAAAACAGT ACTCAGGCAT CTGAGGGAAG ACCAGCATAC TGTGCTTGCT 
TACTTATATG AGATTAGAAC AAGTTTCCAG GAGTTTCTAA AGCGTCCAGA TCACAAGCAA 
GATTTTGTAG CTCAATGGCA GGCTGATTTC AATTCCCTTC CTGATGACCT GTGGGATGAT 
GAGGAAACAA AGGCTGAACT ACACCAACGA GTGAATGATC TGCGAGACCG CCTGTGGGAC 
ATTTGTGACG CACGGAAGGA AGAGGCAGAG CAGGAGCGGC TTGACATCAT TAATGAGAAC 
TGGTTACAGG ACTCTCTTGG AATGACAATG AACCATTTCT TTTCCCTGAT GCAGGCAGAA 
CTGAACCGTT TCCAAGATAC GAAGAGACTC CTTCAAGATT ATTACCGGGG AATGGAAAGT 
AAAATCCCAA TAGAGGACAA TAAGAGATTT ACTCAAATCC CTTTGGTCCA GCTGGATAAT 
AAAGACAATT CTGAAAGCCA GCTTAGAATT CCTCTATTTC CTCGAATATC CATTTCTCTG 
GAAACAGTTA CGCCCAAACC AAAAACAAAA TCAATACTGA AGGGCAAGAT GGATAACTCC 
CTAGAAAACG TTGAGTCCAA CTTTGAGGCT GATGAAAAGT TGGTCATGGA CACCTGGCAA 
CAGGCTTCTT TAGCAGTATC TCACATGGTG GCTGCAGAAA TTCATCAGAG GCTCATGGAA 
GAAGAAAAAG AAAACCAGCC AGCAGACCCC AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT 
AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA AAACAGGAAG ACAAAAAACT CAAAGGTAAA 
TCACCACCTA TTGCAGAAGC AACTCCTGTC ATAGTAACAA CAGAGGAAAT TGCTGAAATC 
GAAAGGAAAA ATGAACTGAG GCTCAAAATA AAAGAAGAAC ACCTTGCTGC CTTGCAATTT 
GAAGAAACAG CCACACAGTT TCGACTTGAA CTGATAAAGA CAAAAGCATT GGCTCTTCTT 
GAAGATTTAG TAACAAAGGT GGTTGATGTA TATAAACTCA TGGAAAAATG GCTTGGTGAG 
AGATATTTGA ATGAAATGGC CAGTGTAGAA AAATTAACTG ACATAGCTCG CTATCACATT 
GAAACATCTA CAAAAATTCA GAATGAACTT TATTTAAACC AAGAAGACTT CTTCATTAAT 
GGCAATGTAA AAGTCTTTCC AGATCCTCCC CCACCGATAC ATCCTCCACC TGTAGAAAAG 
GAAGAAGATG GCACCCTGAC CATCGAACAG CTTGACAATC TTCGGGATCA GTTCTTAGAT 
ATGGCACCTA AAGGCATAAT AGGAAATAAA GCATTTACTG ACATTCTGAT CGATTTGGTG 
ACCCTGAACC TTGGCACAAA CAACTTTCCT AGTAGTTGGA TGCACCTTAC CCAACCTGAA 
TTACAGGAAT TAACATCTTT ATTAACAGTC AACTCTGAGT TTGTGGACTG GCGGAAGTTC 
CTGTTAGTAA CCTCAATGCC TTGGCCCATT CCCTTGGAGG AGGAACTCCT TGAGACCCTT 
CAGAAATTCA AGGCTGTGGA TAAGGCGCAG TTGGGCACCA TCACTTTCGA GCAGTATATG 
CAGGCTGGTC TGTGGTTTAC AGGAGATGAA GATATAAAAA TTCCAGACAA TCCTCTTGAA 
CCCCTGCCAT TTAATAGGCA GGAGCATCTT ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC 
TATGAGAAGG ATCCACCCCA ACTTGACTAC ACACAGATGC TGCTTTACTT TGCTTGCCAC 
CCAGACACCG TGGAAGGAGT CTACAGGGCC CTCAGTGTGG CTGTTGGGAC TCATGTCTTC 
CAACAAGTTG AAGCTTCCAC TCCAAATGCA GAAAAGACCT CCTCAACTGA TGCAAGTCCA 
GCTGAGGAAT TCCCTGAACC TGAGGAAAAT GCTGCAAAAG AAGAAAGGAA ATTAAAAGAC 
AACAGGGAGG AAAGGGAACA GAAGGATGAA GAAATCCCTG AAAATGCAAA CAATGAAAAG 
ATATCCATGG AAACATTACT CAAAGTGTTC AAAGGGGGAA GTGAAGCACA GGACTCTAAC 
AGATTTGCCA GCCACCTAAA GATAGAGAAC ATTTATGCAG AGGGCTTCAT AAAAACATTT 
CAAGACCTAG GTGCCAAGAA CCTGGAGCCA ATTGAAGTTG CTGTTCTCTT GAAGCATCCT 
TTTATTCAAG ACCTGATTTC AAATTATTCA GACTATAAGT TTCCTTGGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_007961358.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007959549.1 Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X12, mRNA.

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CloneID	OCh83468
Clone ID Related Accession (Same CDS sequence)	XM_007961358.1
Accession Version	XM_007961358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	sperm flagellar protein 2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735239.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG CAAGTGATCC ACAAAATGTA 
TTTTCAGCTG GTCCAGTTTC AAATGAAGGA TTACCAGAAA CAGAAGGTGA AACGATACTA 
AGTGCTAATG CTGATAAAAC ACCAAAAGCT GAAGAAATCA AATCCAGTGA CAGTTTCTTA 
AAACTCACTA CGCGTGCTCA ACTTGGTGCA AAATCAGAAC AGTTGCTGAA GAAAGGAAAG 
AGCATTCCTG ACGTGCTGCT TGTTGACATC ATGGTAAATG CTATTAACGA GATACCTGTG 
AATCAGCACT GGATCCTAGA TGGTTTTCCA ATGACTTTAA ATCAAGCACA GCTTCTGGAA 
GAAGCTCTTA CAGGCTACAA TAGAAACCTC ACAGAACTGG AGAGAAAAAA AGCACAAAAA 
TCCACATTGG CTATTGATCC TGCGACTTCC AAAGAAGTAC CTCTTCCCTC TCCTGCATTT 
GATTTTGTCG TATTATTAGA CGTTTCAGAT ACTTCCTCAA TGAGTCGCAT GAAGGATATA 
ATAGCTGAAG AATTGTCCTA TAAAACTGCT CACGAAGACA TCAGTCAACG TGTAGCTGCT 
GAAAATCAAG ATAAGGGTGG AGACCAAAAT TTAAGAGACC AGATACAACA TAGAATTATA 
GGCTTCTTGG ACAACTGGCC TTTATTGAAG CAATGGTTTT CAGAGCCAGA AAATATTTTG 
ATAAAAATCA ATGCTGAAAT AGATAAGGAA TCTTTATGCG AAAAAGTAAA AGAAATTCTT 
ACGACTGAAA TAGTGAAAAA AAAGAATAAA GTTGAGAAGA AATTAGAAGA AAAGGAAGCT 
GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG CTTCCACTTC CTACACCTCC TCCTGCTCCT 
CCTCCTGAAC CAGAAAAAGA GAAGGAAATT CATCTTCATC ATGTGCCTTC AAAAACTCCT 
ACTGCAAAAG GAAAACCTCA ATCAGAAGCC CCGCATGGTA AGCAAGAATC TCTTCAGGAA 
GGAAAAGGGA AGAAAGGTGA AACGGCACTC AAAAGAAAAG GTTCTCCTAA AGGAAAATCA 
TCAGGAGGAA AAGTACCAGT AAAGAAATCA CCTGCTGACT CTACAGACAC ATCACCTGTT 
GCAATAGTGC CGCAGCCACC TAAGCCAGGA TCAGAAGAAT GGGTCTATGT GAATGAACCA 
GTTCCTGAGG AAATGCCTTC GTTTTTAGTA CCTTACTGGG AACTAATAGA AAATTCCTAT 
ATAAACACCA TCAAAACAGT ACTCAGGCAT CTGAGGGAAG ACCAGCATAC TGTGCTTGCT 
TACTTATATG AGATTAGAAC AAGTTTCCAG GAGTTTCTAA AGCGTCCAGA TCACAAGCAA 
GATTTTGTAG CTCAATGGCA GGCTGATTTC AATTCCCTTC CTGATGACCT GTGGGATGAT 
GAGGAAACAA AGGCTGAACT ACACCAACGA GTGAATGATC TGCGAGACCG CCTGTGGGAC 
ATTTGTGACG CACGGAAGGA AGAGGCAGAG CAGGAGCGGC TTGACATCAT TAATGAGAAC 
TGGTTACAGG ACTCTCTTGG AATGACAATG AACCATTTCT TTTCCCTGAT GCAGGCAGAA 
CTGAACCGTT TCCAAGATAC GAAGAGACTC CTTCAAGATT ATTACCGGGG AATGGAAAGT 
AAAATCCCAA TAGAGGACAA TAAGAGATTT ACTCAAATCC CTTTGGTCCA GCTGGATAAT 
AAAGACAATT CTGAAAGCCA GCTTAGAATT CCTCTATTTC CTCGAATATC CATTTCTCTG 
GAAACAGTTA CGCCCAAACC AAAAACAAAA TCAATACTGA AGGGCAAGAT GGATAACTCC 
CTAGAAAACG TTGAGTCCAA CTTTGAGGCT GATGAAAAGT TGGTCATGGA CACCTGGCAA 
CAGGCTTCTT TAGCAGTATC TCACATGGTG GCTGCAGAAA TTCATCAGAG GCTCATGGAA 
GAAGAAAAAG AAAACCAGCC AGCAGACCCC AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT 
AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA AAACAGGAAG ACAAAAAACT CAAAGGTAAA 
TCACCACCTA TTGCAGAAGC AACTCCTGTC ATAGTAACAA CAGAGGAAAT TGCTGAAATC 
GAAAGGAAAA ATGAACTGAG GCTCAAAATA AAAGAAGAAC ACCTTGCTGC CTTGCAATTT 
GAAGAAACAG CCACACAGTT TCGACTTGAA CTGATAAAGA CAAAAGCATT GGCTCTTCTT 
GAAGATTTAG TAACAAAGGT GGTTGATGTA TATAAACTCA TGGAAAAATG GCTTGGTGAG 
AGATATTTGA ATGAAATGGC CAGTGTAGAA AAATTAACTG ACATAGCTCG CTATCACATT 
GAAACATCTA CAAAAATTCA GAATGAACTT TATTTAAACC AAGAAGACTT CTTCATTAAT 
GGCAATGTAA AAGTCTTTCC AGATCCTCCC CCACCGATAC ATCCTCCACC TGTAGAAAAG 
GAAGAAGATG GCACCCTGAC CATCGAACAG CTTGACAATC TTCGGGATCA GTTCTTAGAT 
ATGGCACCTA AAGGCATAAT AGGAAATAAA GCATTTACTG ACATTCTGAT CGATTTGGTG 
ACCCTGAACC TTGGCACAAA CAACTTTCCT AGTAGTTGGA TGCACCTTAC CCAACCTGAA 
TTACAGGAAT TAACATCTTT ATTAACAGTC AACTCTGAGT TTGTGGACTG GCGGAAGTTC 
CTGTTAGTAA CCTCAATGCC TTGGCCCATT CCCTTGGAGG AGGAACTCCT TGAGACCCTT 
CAGAAATTCA AGGCTGTGGA TAAGGCGCAG TTGGGCACCA TCACTTTCGA GCAGTATATG 
CAGGCTGGTC TGTGGTTTAC AGGAGATGAA GATATAAAAA TTCCAGACAA TCCTCTTGAA 
CCCCTGCCAT TTAATAGGCA GGAGCATCTT ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC 
TATGAGAAGG ATCCACCCCA ACTTGACTAC ACACAGATGC TGCTTTACTT TGCTTGCCAC 
CCAGACACCG TGGAAGGAGT CTACAGGGCC CTCAGTGTGG CTGTTGGGAC TCATGTCTTC 
CAACAAGTTG AAGCTTCCAC TCCAAATGCA GAAAAGACCT CCTCAACTGA TGCAAGTCCA 
GCTGAGGAAT TCCCTGAACC TGAGGAAAAT GCTGCAAAAG AAGAAAGGAA ATTAAAAGAC 
AACAGGGAGG AAAGGGAACA GAAGGATGAA GAAATCCCTG AAAATGCAAA CAATGAAAAG 
ATATCCATGG AAACATTACT CAAAGTGTTC AAAGGGGGAA GTGAAGCACA GGACTCTAAC 
AGATTTGCCA GCCACCTAAA GATAGAGAAC ATTTATGCAG AGGGCTTCAT AAAAACATTT 
CAAGACCTAG GTGCCAAGAA CCTGGAGCCA ATTGAAGTTG CTGTTCTCTT GAAGCATCCT 
TTTATTCAAG ACCTGATTTC AAATTATTCA GACTATAAGT TTCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007959549.1
CDS	107..5494
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGFLLGEVLYKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQPLTNLRLQHMKSETFRERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIKKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVANEIKKFEALIKKDLQAKESASKTTLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYKKHYSICAEILDQIVDLSTKVADYRTLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLIELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPMRILSIDTLVQEAIQAFHDNEKVSEILPIQKKDEEDALPVLQEEIKESQASDPQNVFSAGPVSNEGLPETEGETILSANADKTPKAEEIKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINEIPVNQHWILDGFPMTLNQAQLLEEALTGYNRNLTELERKKAQKSTLAIDPATSKEVPLPSPAFDFVVLLDVSDTSSMSRMKDIIAEELSYKTAHEDISQRVAAENQDKGGDQNLRDQIQHRIIGFLDNWPLLKQWFSEPENILIKINAEIDKESLCEKVKEILTTEIVKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHLHHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINENWLQDSLGMTMNHFFSLMQAELNRFQDTKRLLQDYYRGMESKIPIEDNKRFTQIPLVQLDNKDNSESQLRIPLFPRISISLETVTPKPKTKSILKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKLKGKSPPIAEATPVIVTTEEIAEIERKNELRLKIKEEHLAALQFEETATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASVEKLTDIARYHIETSTKIQNELYLNQEDFFINGNVKVFPDPPPPIHPPPVEKEEDGTLTIEQLDNLRDQFLDMAPKGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKAQLGTITFEQYMQAGLWFTGDEDIKIPDNPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVEASTPNAEKTSSTDASPAEEFPEPEENAAKEERKLKDNREEREQKDEEIPENANNEKISMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFP

Target ORF information:

RefSeq Version	XM_007961358.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007961358.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG CAAGTGATCC ACAAAATGTA 
TTTTCAGCTG GTCCAGTTTC AAATGAAGGA TTACCAGAAA CAGAAGGTGA AACGATACTA 
AGTGCTAATG CTGATAAAAC ACCAAAAGCT GAAGAAATCA AATCCAGTGA CAGTTTCTTA 
AAACTCACTA CGCGTGCTCA ACTTGGTGCA AAATCAGAAC AGTTGCTGAA GAAAGGAAAG 
AGCATTCCTG ACGTGCTGCT TGTTGACATC ATGGTAAATG CTATTAACGA GATACCTGTG 
AATCAGCACT GGATCCTAGA TGGTTTTCCA ATGACTTTAA ATCAAGCACA GCTTCTGGAA 
GAAGCTCTTA CAGGCTACAA TAGAAACCTC ACAGAACTGG AGAGAAAAAA AGCACAAAAA 
TCCACATTGG CTATTGATCC TGCGACTTCC AAAGAAGTAC CTCTTCCCTC TCCTGCATTT 
GATTTTGTCG TATTATTAGA CGTTTCAGAT ACTTCCTCAA TGAGTCGCAT GAAGGATATA 
ATAGCTGAAG AATTGTCCTA TAAAACTGCT CACGAAGACA TCAGTCAACG TGTAGCTGCT 
GAAAATCAAG ATAAGGGTGG AGACCAAAAT TTAAGAGACC AGATACAACA TAGAATTATA 
GGCTTCTTGG ACAACTGGCC TTTATTGAAG CAATGGTTTT CAGAGCCAGA AAATATTTTG 
ATAAAAATCA ATGCTGAAAT AGATAAGGAA TCTTTATGCG AAAAAGTAAA AGAAATTCTT 
ACGACTGAAA TAGTGAAAAA AAAGAATAAA GTTGAGAAGA AATTAGAAGA AAAGGAAGCT 
GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG CTTCCACTTC CTACACCTCC TCCTGCTCCT 
CCTCCTGAAC CAGAAAAAGA GAAGGAAATT CATCTTCATC ATGTGCCTTC AAAAACTCCT 
ACTGCAAAAG GAAAACCTCA ATCAGAAGCC CCGCATGGTA AGCAAGAATC TCTTCAGGAA 
GGAAAAGGGA AGAAAGGTGA AACGGCACTC AAAAGAAAAG GTTCTCCTAA AGGAAAATCA 
TCAGGAGGAA AAGTACCAGT AAAGAAATCA CCTGCTGACT CTACAGACAC ATCACCTGTT 
GCAATAGTGC CGCAGCCACC TAAGCCAGGA TCAGAAGAAT GGGTCTATGT GAATGAACCA 
GTTCCTGAGG AAATGCCTTC GTTTTTAGTA CCTTACTGGG AACTAATAGA AAATTCCTAT 
ATAAACACCA TCAAAACAGT ACTCAGGCAT CTGAGGGAAG ACCAGCATAC TGTGCTTGCT 
TACTTATATG AGATTAGAAC AAGTTTCCAG GAGTTTCTAA AGCGTCCAGA TCACAAGCAA 
GATTTTGTAG CTCAATGGCA GGCTGATTTC AATTCCCTTC CTGATGACCT GTGGGATGAT 
GAGGAAACAA AGGCTGAACT ACACCAACGA GTGAATGATC TGCGAGACCG CCTGTGGGAC 
ATTTGTGACG CACGGAAGGA AGAGGCAGAG CAGGAGCGGC TTGACATCAT TAATGAGAAC 
TGGTTACAGG ACTCTCTTGG AATGACAATG AACCATTTCT TTTCCCTGAT GCAGGCAGAA 
CTGAACCGTT TCCAAGATAC GAAGAGACTC CTTCAAGATT ATTACCGGGG AATGGAAAGT 
AAAATCCCAA TAGAGGACAA TAAGAGATTT ACTCAAATCC CTTTGGTCCA GCTGGATAAT 
AAAGACAATT CTGAAAGCCA GCTTAGAATT CCTCTATTTC CTCGAATATC CATTTCTCTG 
GAAACAGTTA CGCCCAAACC AAAAACAAAA TCAATACTGA AGGGCAAGAT GGATAACTCC 
CTAGAAAACG TTGAGTCCAA CTTTGAGGCT GATGAAAAGT TGGTCATGGA CACCTGGCAA 
CAGGCTTCTT TAGCAGTATC TCACATGGTG GCTGCAGAAA TTCATCAGAG GCTCATGGAA 
GAAGAAAAAG AAAACCAGCC AGCAGACCCC AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT 
AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA AAACAGGAAG ACAAAAAACT CAAAGGTAAA 
TCACCACCTA TTGCAGAAGC AACTCCTGTC ATAGTAACAA CAGAGGAAAT TGCTGAAATC 
GAAAGGAAAA ATGAACTGAG GCTCAAAATA AAAGAAGAAC ACCTTGCTGC CTTGCAATTT 
GAAGAAACAG CCACACAGTT TCGACTTGAA CTGATAAAGA CAAAAGCATT GGCTCTTCTT 
GAAGATTTAG TAACAAAGGT GGTTGATGTA TATAAACTCA TGGAAAAATG GCTTGGTGAG 
AGATATTTGA ATGAAATGGC CAGTGTAGAA AAATTAACTG ACATAGCTCG CTATCACATT 
GAAACATCTA CAAAAATTCA GAATGAACTT TATTTAAACC AAGAAGACTT CTTCATTAAT 
GGCAATGTAA AAGTCTTTCC AGATCCTCCC CCACCGATAC ATCCTCCACC TGTAGAAAAG 
GAAGAAGATG GCACCCTGAC CATCGAACAG CTTGACAATC TTCGGGATCA GTTCTTAGAT 
ATGGCACCTA AAGGCATAAT AGGAAATAAA GCATTTACTG ACATTCTGAT CGATTTGGTG 
ACCCTGAACC TTGGCACAAA CAACTTTCCT AGTAGTTGGA TGCACCTTAC CCAACCTGAA 
TTACAGGAAT TAACATCTTT ATTAACAGTC AACTCTGAGT TTGTGGACTG GCGGAAGTTC 
CTGTTAGTAA CCTCAATGCC TTGGCCCATT CCCTTGGAGG AGGAACTCCT TGAGACCCTT 
CAGAAATTCA AGGCTGTGGA TAAGGCGCAG TTGGGCACCA TCACTTTCGA GCAGTATATG 
CAGGCTGGTC TGTGGTTTAC AGGAGATGAA GATATAAAAA TTCCAGACAA TCCTCTTGAA 
CCCCTGCCAT TTAATAGGCA GGAGCATCTT ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC 
TATGAGAAGG ATCCACCCCA ACTTGACTAC ACACAGATGC TGCTTTACTT TGCTTGCCAC 
CCAGACACCG TGGAAGGAGT CTACAGGGCC CTCAGTGTGG CTGTTGGGAC TCATGTCTTC 
CAACAAGTTG AAGCTTCCAC TCCAAATGCA GAAAAGACCT CCTCAACTGA TGCAAGTCCA 
GCTGAGGAAT TCCCTGAACC TGAGGAAAAT GCTGCAAAAG AAGAAAGGAA ATTAAAAGAC 
AACAGGGAGG AAAGGGAACA GAAGGATGAA GAAATCCCTG AAAATGCAAA CAATGAAAAG 
ATATCCATGG AAACATTACT CAAAGTGTTC AAAGGGGGAA GTGAAGCACA GGACTCTAAC 
AGATTTGCCA GCCACCTAAA GATAGAGAAC ATTTATGCAG AGGGCTTCAT AAAAACATTT 
CAAGACCTAG GTGCCAAGAA CCTGGAGCCA ATTGAAGTTG CTGTTCTCTT GAAGCATCCT 
TTTATTCAAG ACCTGATTTC AAATTATTCA GACTATAAGT TTCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_007961359.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007959550.1 Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X13, mRNA.

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CloneID	OCh83469
Clone ID Related Accession (Same CDS sequence)	XM_007961359.1
Accession Version	XM_007961359.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	sperm flagellar protein 2 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735239.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG CAAGTGATCC ACAAAATGTA 
TTTTCAGCTG GTCCAGTTTC AAATGAAGGA TTACCAGAAA CAGAAGGTGA AACGATACTA 
AGTGCTAATG CTGATAAAAC ACCAAAAGCT GAAGAAATCA AATCCAGTGA CAGTTTCTTA 
AAACTCACTA CGCGTGCTCA ACTTGGTGCA AAATCAGAAC AGTTGCTGAA GAAAGGAAAG 
AGCATTCCTG ACGTGCTGCT TGTTGACATC ATGGTAAATG CTATTAACGA GATACCTGTG 
AATCAGCACT GGATCCTAGA TGGTTTTCCA ATGACTTTAA ATCAAGCACA GCTTCTGGAA 
GAAGCTCTTA CAGGCTACAA TAGAAACCTC ACAGAACTGG AGAGAAAAAA AGCACAAAAA 
TCCACATTGG CTATTGATCC TGCGACTTCC AAAGAAGTAC CTCTTCCCTC TCCTGCATTT 
GATTTTGTCG TATTATTAGA CGTTTCAGAT ACTTCCTCAA TGAGTCGCAT GAAGGATATA 
ATAGCTGAAG AATTGTCCTA TAAAACTGCT CACGAAGACA TCAGTCAACG TGTAGCTGCT 
GAAAATCAAG ATAAGGGTGG AGACCAAAAT TTAAGAGACC AGATACAACA TAGAATTATA 
GGCTTCTTGG ACAACTGGCC TTTATTGAAG CAATGGTTTT CAGAGCCAGA AAATATTTTG 
ATAAAAATCA ATGCTGAAAT AGATAAGGAA TCTTTATGCG AAAAAGTAAA AGAAATTCTT 
ACGACTGAAA TAGTGAAAAA AAAGAATAAA GTTGAGAAGA AATTAGAAGA AAAGGAAGCT 
GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG CTTCCACTTC CTACACCTCC TCCTGCTCCT 
CCTCCTGAAC CAGAAAAAGA GAAGGAAATT CATCTTCATC ATGTGCCTTC AAAAACTCCT 
ACTGCAAAAG GAAAACCTCA ATCAGAAGCC CCGCATGGTA AGCAAGAATC TCTTCAGGAA 
GGAAAAGGGA AGAAAGGTGA AACGGCACTC AAAAGAAAAG GTTCTCCTAA AGGAAAATCA 
TCAGGAGGAA AAGTACCAGT AAAGAAATCA CCTGCTGACT CTACAGACAC ATCACCTGTT 
GCAATAGTGC CGCAGCCACC TAAGCCAGGA TCAGAAGAAT GGGTCTATGT GAATGAACCA 
GTTCCTGAGG AAATGCCTTC GTTTTTAGTA CCTTACTGGG AACTAATAGA AAATTCCTAT 
ATAAACACCA TCAAAACAGT ACTCAGGCAT CTGAGGGAAG ACCAGCATAC TGTGCTTGCT 
TACTTATATG AGATTAGAAC AAGTTTCCAG GAGTTTCTAA AGCGTCCAGA TCACAAGCAA 
GATTTTGTAG CTCAATGGCA GGCTGATTTC AATTCCCTTC CTGATGACCT GTGGGATGAT 
GAGGAAACAA AGGCTGAACT ACACCAACGA GTGAATGATC TGCGAGACCG CCTGTGGGAC 
ATTTGTGACG CACGGAAGGA AGAGGCAGAG CAGGAGCGGC TTGACATCAT TAATGAGAAC 
TGGTTACAGG ACTCTCTTGG AATGACAATG AACCATTTCT TTTCCCTGAT GCAGGCAGAA 
CTGAACCGTT TCCAAGATAC GAAGAGACTC CTTCAAGATT ATTACCGGGG AATGGAAAGT 
AAAATCCCAA TAGAGGACAA TAAGAGATTT ACTCAAATCC CTTTGGTCCA GCTGGATAAT 
AAAGACAATT CTGAAAGCCA GCTTAGAATT CCTCTATTTC CTCGAATATC CATTTCTCTG 
GAAACAGTTA CGCCCAAACC AAAAACAAAA TCAATACTGA AGGGCAAGAT GGATAACTCC 
CTAGAAAACG TTGAGTCCAA CTTTGAGGCT GATGAAAAGT TGGTCATGGA CACCTGGCAA 
CAGGCTTCTT TAGCAGTATC TCACATGGTG GCTGCAGAAA TTCATCAGAG GCTCATGGAA 
GAAGAAAAAG AAAACCAGCC AGCAGACCCC AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT 
AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA AAACAGGAAG ACAAAAAACT CAAAGGTAAA 
TCACCACCTA TTGCAGAAGC AACTCCTGTC ATAGTAACAA CAGAGGAAAT TGCTGAAATC 
GAAAGGAAAA ATGAACTGAG GCTCAAAATA AAAGAAGAAC ACCTTGCTGC CTTGCAATTT 
GAAGAAACAG CCACACAGTT TCGACTTGAA CTGATAAAGA CAAAAGCATT GGCTCTTCTT 
GAAGATTTAG TAACAAAGGT GGTTGATGTA TATAAACTCA TGGAAAAATG GCTTGGTGAG 
AGATATTTGA ATGAAATGGC CAGTGTAGAA AAATTAACTG ACATAGCTCG CTATCACATT 
GAAACATCTA CAAAAATTCA GAATGAACTT TATTTAAACC AAGAAGACTT CTTCATTAAT 
GGCAATGTAA AAGTCTTTCC AGATCCTCCC CCACCGATAC ATCCTCCACC TGTAGAAAAG 
GAAGAAGATG GCACCCTGAC CATCGAACAG CTTGACAATC TTCGGGATCA GTTCTTAGAT 
ATGGCACCTA AAGGACTCAA TTTCTCGATG CACATCAACT CAGCGAGGCT CATGCCAAGG 
CTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007959550.1
CDS	107..4615
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGFLLGEVLYKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQPLTNLRLQHMKSETFRERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIKKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVANEIKKFEALIKKDLQAKESASKTTLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYKKHYSICAEILDQIVDLSTKVADYRTLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLIELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPMRILSIDTLVQEAIQAFHDNEKVSEILPIQKKDEEDALPVLQEEIKESQASDPQNVFSAGPVSNEGLPETEGETILSANADKTPKAEEIKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINEIPVNQHWILDGFPMTLNQAQLLEEALTGYNRNLTELERKKAQKSTLAIDPATSKEVPLPSPAFDFVVLLDVSDTSSMSRMKDIIAEELSYKTAHEDISQRVAAENQDKGGDQNLRDQIQHRIIGFLDNWPLLKQWFSEPENILIKINAEIDKESLCEKVKEILTTEIVKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHLHHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINENWLQDSLGMTMNHFFSLMQAELNRFQDTKRLLQDYYRGMESKIPIEDNKRFTQIPLVQLDNKDNSESQLRIPLFPRISISLETVTPKPKTKSILKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKLKGKSPPIAEATPVIVTTEEIAEIERKNELRLKIKEEHLAALQFEETATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASVEKLTDIARYHIETSTKIQNELYLNQEDFFINGNVKVFPDPPPPIHPPPVEKEEDGTLTIEQLDNLRDQFLDMAPKGLNFSMHINSARLMPRLH

Target ORF information:

RefSeq Version	XM_007961359.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007961359.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG CAAGTGATCC ACAAAATGTA 
TTTTCAGCTG GTCCAGTTTC AAATGAAGGA TTACCAGAAA CAGAAGGTGA AACGATACTA 
AGTGCTAATG CTGATAAAAC ACCAAAAGCT GAAGAAATCA AATCCAGTGA CAGTTTCTTA 
AAACTCACTA CGCGTGCTCA ACTTGGTGCA AAATCAGAAC AGTTGCTGAA GAAAGGAAAG 
AGCATTCCTG ACGTGCTGCT TGTTGACATC ATGGTAAATG CTATTAACGA GATACCTGTG 
AATCAGCACT GGATCCTAGA TGGTTTTCCA ATGACTTTAA ATCAAGCACA GCTTCTGGAA 
GAAGCTCTTA CAGGCTACAA TAGAAACCTC ACAGAACTGG AGAGAAAAAA AGCACAAAAA 
TCCACATTGG CTATTGATCC TGCGACTTCC AAAGAAGTAC CTCTTCCCTC TCCTGCATTT 
GATTTTGTCG TATTATTAGA CGTTTCAGAT ACTTCCTCAA TGAGTCGCAT GAAGGATATA 
ATAGCTGAAG AATTGTCCTA TAAAACTGCT CACGAAGACA TCAGTCAACG TGTAGCTGCT 
GAAAATCAAG ATAAGGGTGG AGACCAAAAT TTAAGAGACC AGATACAACA TAGAATTATA 
GGCTTCTTGG ACAACTGGCC TTTATTGAAG CAATGGTTTT CAGAGCCAGA AAATATTTTG 
ATAAAAATCA ATGCTGAAAT AGATAAGGAA TCTTTATGCG AAAAAGTAAA AGAAATTCTT 
ACGACTGAAA TAGTGAAAAA AAAGAATAAA GTTGAGAAGA AATTAGAAGA AAAGGAAGCT 
GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG CTTCCACTTC CTACACCTCC TCCTGCTCCT 
CCTCCTGAAC CAGAAAAAGA GAAGGAAATT CATCTTCATC ATGTGCCTTC AAAAACTCCT 
ACTGCAAAAG GAAAACCTCA ATCAGAAGCC CCGCATGGTA AGCAAGAATC TCTTCAGGAA 
GGAAAAGGGA AGAAAGGTGA AACGGCACTC AAAAGAAAAG GTTCTCCTAA AGGAAAATCA 
TCAGGAGGAA AAGTACCAGT AAAGAAATCA CCTGCTGACT CTACAGACAC ATCACCTGTT 
GCAATAGTGC CGCAGCCACC TAAGCCAGGA TCAGAAGAAT GGGTCTATGT GAATGAACCA 
GTTCCTGAGG AAATGCCTTC GTTTTTAGTA CCTTACTGGG AACTAATAGA AAATTCCTAT 
ATAAACACCA TCAAAACAGT ACTCAGGCAT CTGAGGGAAG ACCAGCATAC TGTGCTTGCT 
TACTTATATG AGATTAGAAC AAGTTTCCAG GAGTTTCTAA AGCGTCCAGA TCACAAGCAA 
GATTTTGTAG CTCAATGGCA GGCTGATTTC AATTCCCTTC CTGATGACCT GTGGGATGAT 
GAGGAAACAA AGGCTGAACT ACACCAACGA GTGAATGATC TGCGAGACCG CCTGTGGGAC 
ATTTGTGACG CACGGAAGGA AGAGGCAGAG CAGGAGCGGC TTGACATCAT TAATGAGAAC 
TGGTTACAGG ACTCTCTTGG AATGACAATG AACCATTTCT TTTCCCTGAT GCAGGCAGAA 
CTGAACCGTT TCCAAGATAC GAAGAGACTC CTTCAAGATT ATTACCGGGG AATGGAAAGT 
AAAATCCCAA TAGAGGACAA TAAGAGATTT ACTCAAATCC CTTTGGTCCA GCTGGATAAT 
AAAGACAATT CTGAAAGCCA GCTTAGAATT CCTCTATTTC CTCGAATATC CATTTCTCTG 
GAAACAGTTA CGCCCAAACC AAAAACAAAA TCAATACTGA AGGGCAAGAT GGATAACTCC 
CTAGAAAACG TTGAGTCCAA CTTTGAGGCT GATGAAAAGT TGGTCATGGA CACCTGGCAA 
CAGGCTTCTT TAGCAGTATC TCACATGGTG GCTGCAGAAA TTCATCAGAG GCTCATGGAA 
GAAGAAAAAG AAAACCAGCC AGCAGACCCC AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT 
AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA AAACAGGAAG ACAAAAAACT CAAAGGTAAA 
TCACCACCTA TTGCAGAAGC AACTCCTGTC ATAGTAACAA CAGAGGAAAT TGCTGAAATC 
GAAAGGAAAA ATGAACTGAG GCTCAAAATA AAAGAAGAAC ACCTTGCTGC CTTGCAATTT 
GAAGAAACAG CCACACAGTT TCGACTTGAA CTGATAAAGA CAAAAGCATT GGCTCTTCTT 
GAAGATTTAG TAACAAAGGT GGTTGATGTA TATAAACTCA TGGAAAAATG GCTTGGTGAG 
AGATATTTGA ATGAAATGGC CAGTGTAGAA AAATTAACTG ACATAGCTCG CTATCACATT 
GAAACATCTA CAAAAATTCA GAATGAACTT TATTTAAACC AAGAAGACTT CTTCATTAAT 
GGCAATGTAA AAGTCTTTCC AGATCCTCCC CCACCGATAC ATCCTCCACC TGTAGAAAAG 
GAAGAAGATG GCACCCTGAC CATCGAACAG CTTGACAATC TTCGGGATCA GTTCTTAGAT 
ATGGCACCTA AAGGACTCAA TTTCTCGATG CACATCAACT CAGCGAGGCT CATGCCAAGG 
CTTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_007961362.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007959553.1 Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X17, mRNA.

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CloneID	OCh83472
Clone ID Related Accession (Same CDS sequence)	XM_007961362.1
Accession Version	XM_007961362.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	sperm flagellar protein 2 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735239.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAAAA TGTATTTTCA 
GCTGGTCCAG TTTCAAATGA AGGATTACCA GAAACAGAAG GTGAAACGAT ACTAAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA ATCAAATCCA GTGACAGTTT CTTAAAACTC 
ACTACGCGTG CTCAACTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGACGTGC TGCTTGTTGA CATCATGGTA AATGCTATTA ACGAGATACC TGTGAATCAG 
CACTGGATCC TAGATGGTTT TCCAATGACT TTAAATCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT ACAATAGAAA CCTCACAGAA CTGGAGAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA GTACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCGTATTAT TAGACGTTTC AGATACTTCC TCAATGAGTC GCATGAAGGA TATAATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GACATCAGTC AACGTGTAGC TGCTGAAAAT 
CAAGATAAGG GTGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GAAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGCGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGTGA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCTT CATCATGTGC CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAAACGGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ACACATCACC TGTTGCAATA 
GTGCCGCAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTCGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CAGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAATTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACACCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GACGCACGGA AGGAAGAGGC AGAGCAGGAG CGGCTTGACA TCATTAATGA GAACTGGTTA 
CAGGACTCTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAACTGAAC 
CGTTTCCAAG ATACGAAGAG ACTCCTTCAA GATTATTACC GGGGAATGGA AAGTAAAATC 
CCAATAGAGG ACAATAAGAG ATTTACTCAA ATCCCTTTGG TCCAGCTGGA TAATAAAGAC 
AATTCTGAAA GCCAGCTTAG AATTCCTCTA TTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACGCCCA AACCAAAAAC AAAATCAATA CTGAAGGGCA AGATGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCTGATGAA AAGTTGGTCA TGGACACCTG GCAACAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCA GAAATTCATC AGAGGCTCAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACTCAAAGG TAAATCACCA 
CCTATTGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCGAAAGG 
AAAAATGAAC TGAGGCTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ACAGCCACAC AGTTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGATAT 
TTGAATGAAA TGGCCAGTGT AGAAAAATTA ACTGACATAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AACCAAGAAG ACTTCTTCAT TAATGGCAAT 
GTAAAAGTCT TTCCAGATCC TCCCCCACCG ATACATCCTC CACCTGTAGA AAAGGAAGAA 
GATGGCACCC TGACCATCGA ACAGCTTGAC AATCTTCGGG ATCAGTTCTT AGATATGGCA 
CCTAAAGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG 
AACCTTGGCA CAAACAACTT TCCTAGTAGT TGGATGCACC TTACCCAACC TGAATTACAG 
GAATTAACAT CTTTATTAAC AGTCAACTCT GAGTTTGTGG ACTGGCGGAA GTTCCTGTTA 
GTAACCTCAA TGCCTTGGCC CATTCCCTTG GAGGAGGAAC TCCTTGAGAC CCTTCAGAAA 
TTCAAGGCTG TGGATAAGGC GCAGTTGGGC ACCATCACTT TCGAGCAGTA TATGCAGGCT 
GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG ACAATCCTCT TGAACCCCTG 
CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATGAG 
AAGGATCCAC CCCAACTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC 
ACCGTGGAAG GAGTCTACAG GGCCCTCAGT GTGGCTGTTG GGACTCATGT CTTCCAACAA 
GTTGAAGCTT CCACTCCAAA TGCAGAAAAG ACCTCCTCAA CTGATGCAAG TCCAGCTGAG 
GAATTCCCTG AACCTGAGGA AAATGCTGCA AAAGAAGAAA GGAAATTAAA AGACAACAGG 
GAGGAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATATCC 
ATGGAAACAT TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC TAACAGATTT 
GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC 
CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTTGCTGTTC TCTTGAAGCA TCCTTTTATT 
CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG ATATTAAAAT AATTCTCCAA 
AGGAGTGAAC ATGTACCAGG AAGTGATGGA GAGAGATCAC CTTTAAGACA TACAGAGGAA 
AAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007959553.1
CDS	107..5575
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGFLLGEVLYKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQPLTNLRLQHMKSETFRERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIKKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVANEIKKFEALIKKDLQAKESASKTTLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYKKHYSICAEILDQIVDLSTKVADYRTLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLIELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPMRILSIDTLVQEAIQAFHDNEKVSEILPIQKKDEEDALPVLQEEIKESQDPQNVFSAGPVSNEGLPETEGETILSANADKTPKAEEIKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINEIPVNQHWILDGFPMTLNQAQLLEEALTGYNRNLTELERKKAQKSTLAIDPATSKEVPLPSPAFDFVVLLDVSDTSSMSRMKDIIAEELSYKTAHEDISQRVAAENQDKGGDQNLRDQIQHRIIGFLDNWPLLKQWFSEPENILIKINAEIDKESLCEKVKEILTTEIVKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHLHHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINENWLQDSLGMTMNHFFSLMQAELNRFQDTKRLLQDYYRGMESKIPIEDNKRFTQIPLVQLDNKDNSESQLRIPLFPRISISLETVTPKPKTKSILKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKLKGKSPPIAEATPVIVTTEEIAEIERKNELRLKIKEEHLAALQFEETATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASVEKLTDIARYHIETSTKIQNELYLNQEDFFINGNVKVFPDPPPPIHPPPVEKEEDGTLTIEQLDNLRDQFLDMAPKGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKAQLGTITFEQYMQAGLWFTGDEDIKIPDNPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVEASTPNAEKTSSTDASPAEEFPEPEENAAKEERKLKDNREEREQKDEEIPENANNEKISMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPDIKIILQRSEHVPGSDGERSPLRHTEEKK

Target ORF information:

RefSeq Version	XM_007961362.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007961362.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAAAA TGTATTTTCA 
GCTGGTCCAG TTTCAAATGA AGGATTACCA GAAACAGAAG GTGAAACGAT ACTAAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA ATCAAATCCA GTGACAGTTT CTTAAAACTC 
ACTACGCGTG CTCAACTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGACGTGC TGCTTGTTGA CATCATGGTA AATGCTATTA ACGAGATACC TGTGAATCAG 
CACTGGATCC TAGATGGTTT TCCAATGACT TTAAATCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT ACAATAGAAA CCTCACAGAA CTGGAGAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA GTACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCGTATTAT TAGACGTTTC AGATACTTCC TCAATGAGTC GCATGAAGGA TATAATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GACATCAGTC AACGTGTAGC TGCTGAAAAT 
CAAGATAAGG GTGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GAAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGCGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGTGA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCTT CATCATGTGC CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAAACGGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ACACATCACC TGTTGCAATA 
GTGCCGCAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTCGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CAGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAATTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACACCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GACGCACGGA AGGAAGAGGC AGAGCAGGAG CGGCTTGACA TCATTAATGA GAACTGGTTA 
CAGGACTCTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAACTGAAC 
CGTTTCCAAG ATACGAAGAG ACTCCTTCAA GATTATTACC GGGGAATGGA AAGTAAAATC 
CCAATAGAGG ACAATAAGAG ATTTACTCAA ATCCCTTTGG TCCAGCTGGA TAATAAAGAC 
AATTCTGAAA GCCAGCTTAG AATTCCTCTA TTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACGCCCA AACCAAAAAC AAAATCAATA CTGAAGGGCA AGATGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCTGATGAA AAGTTGGTCA TGGACACCTG GCAACAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCA GAAATTCATC AGAGGCTCAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACTCAAAGG TAAATCACCA 
CCTATTGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCGAAAGG 
AAAAATGAAC TGAGGCTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ACAGCCACAC AGTTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGATAT 
TTGAATGAAA TGGCCAGTGT AGAAAAATTA ACTGACATAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AACCAAGAAG ACTTCTTCAT TAATGGCAAT 
GTAAAAGTCT TTCCAGATCC TCCCCCACCG ATACATCCTC CACCTGTAGA AAAGGAAGAA 
GATGGCACCC TGACCATCGA ACAGCTTGAC AATCTTCGGG ATCAGTTCTT AGATATGGCA 
CCTAAAGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG 
AACCTTGGCA CAAACAACTT TCCTAGTAGT TGGATGCACC TTACCCAACC TGAATTACAG 
GAATTAACAT CTTTATTAAC AGTCAACTCT GAGTTTGTGG ACTGGCGGAA GTTCCTGTTA 
GTAACCTCAA TGCCTTGGCC CATTCCCTTG GAGGAGGAAC TCCTTGAGAC CCTTCAGAAA 
TTCAAGGCTG TGGATAAGGC GCAGTTGGGC ACCATCACTT TCGAGCAGTA TATGCAGGCT 
GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG ACAATCCTCT TGAACCCCTG 
CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATGAG 
AAGGATCCAC CCCAACTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC 
ACCGTGGAAG GAGTCTACAG GGCCCTCAGT GTGGCTGTTG GGACTCATGT CTTCCAACAA 
GTTGAAGCTT CCACTCCAAA TGCAGAAAAG ACCTCCTCAA CTGATGCAAG TCCAGCTGAG 
GAATTCCCTG AACCTGAGGA AAATGCTGCA AAAGAAGAAA GGAAATTAAA AGACAACAGG 
GAGGAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATATCC 
ATGGAAACAT TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC TAACAGATTT 
GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC 
CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTTGCTGTTC TCTTGAAGCA TCCTTTTATT 
CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG ATATTAAAAT AATTCTCCAA 
AGGAGTGAAC ATGTACCAGG AAGTGATGGA GAGAGATCAC CTTTAAGACA TACAGAGGAA 
AAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_007961346.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007959537.1 Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.

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CloneID	OCh83457
Clone ID Related Accession (Same CDS sequence)	XM_007961346.1
Accession Version	XM_007961346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	sperm flagellar protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735239.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG CAAGTGATCC ACAAAATGTA 
TTTTCAGCTG GTCCAGTTTC AAATGAAGGA TTACCAGAAA CAGAAGGTGA AACGATACTA 
AGTGCTAATG CTGATAAAAC ACCAAAAGCT GAAGAAATCA AATCCAGTGA CAGTTTCTTA 
AAACTCACTA CGCGTGCTCA ACTTGGTGCA AAATCAGAAC AGTTGCTGAA GAAAGGAAAG 
AGCATTCCTG ACGTGCTGCT TGTTGACATC ATGGTAAATG CTATTAACGA GATACCTGTG 
AATCAGCACT GGATCCTAGA TGGTTTTCCA ATGACTTTAA ATCAAGCACA GCTTCTGGAA 
GAAGCTCTTA CAGGCTACAA TAGAAACCTC ACAGAACTGG AGAGAAAAAA AGCACAAAAA 
TCCACATTGG CTATTGATCC TGCGACTTCC AAAGAAGTAC CTCTTCCCTC TCCTGCATTT 
GATTTTGTCG TATTATTAGA CGTTTCAGAT ACTTCCTCAA TGAGTCGCAT GAAGGATATA 
ATAGCTGAAG AATTGTCCTA TAAAACTGCT CACGAAGACA TCAGTCAACG TGTAGCTGCT 
GAAAATCAAG ATAAGGGTGG AGACCAAAAT TTAAGAGACC AGATACAACA TAGAATTATA 
GGCTTCTTGG ACAACTGGCC TTTATTGAAG CAATGGTTTT CAGAGCCAGA AAATATTTTG 
ATAAAAATCA ATGCTGAAAT AGATAAGGAA TCTTTATGCG AAAAAGTAAA AGAAATTCTT 
ACGACTGAAA TAGTGAAAAA AAAGAATAAA GTTGAGAAGA AATTAGAAGA AAAGGAAGCT 
GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG CTTCCACTTC CTACACCTCC TCCTGCTCCT 
CCTCCTGAAC CAGAAAAAGA GAAGGAAATT CATCTTCATC ATGTGCCTTC AAAAACTCCT 
ACTGCAAAAG GAAAACCTCA ATCAGAAGCC CCGCATGGTA AGCAAGAATC TCTTCAGGAA 
GGAAAAGGGA AGAAAGGTGA AACGGCACTC AAAAGAAAAG GTTCTCCTAA AGGAAAATCA 
TCAGGAGGAA AAGTACCAGT AAAGAAATCA CCTGCTGACT CTACAGACAC ATCACCTGTT 
GCAATAGTGC CGCAGCCACC TAAGCCAGGA TCAGAAGAAT GGGTCTATGT GAATGAACCA 
GTTCCTGAGG AAATGCCTTC GTTTTTAGTA CCTTACTGGG AACTAATAGA AAATTCCTAT 
ATAAACACCA TCAAAACAGT ACTCAGGCAT CTGAGGGAAG ACCAGCATAC TGTGCTTGCT 
TACTTATATG AGATTAGAAC AAGTTTCCAG GAGTTTCTAA AGCGTCCAGA TCACAAGCAA 
GATTTTGTAG CTCAATGGCA GGCTGATTTC AATTCCCTTC CTGATGACCT GTGGGATGAT 
GAGGAAACAA AGGCTGAACT ACACCAACGA GTGAATGATC TGCGAGACCG CCTGTGGGAC 
ATTTGTGACG CACGGAAGGA AGAGGCAGAG CAGGAGCGGC TTGACATCAT TAATGAGAAC 
TGGTTACAGG ACTCTCTTGG AATGACAATG AACCATTTCT TTTCCCTGAT GCAGGCAGAA 
CTGAACCGTT TCCAAGATAC GAAGAGACTC CTTCAAGATT ATTACCGGGG AATGGAAAGT 
AAAATCCCAA TAGAGGACAA TAAGAGATTT ACTCAAATCC CTTTGGTCCA GCTGGATAAT 
AAAGACAATT CTGAAAGCCA GCTTAGAATT CCTCTATTTC CTCGAATATC CATTTCTCTG 
GAAACAGTTA CGCCCAAACC AAAAACAAAA TCAATACTGA AGGGCAAGAT GGATAACTCC 
CTAGAAAACG TTGAGTCCAA CTTTGAGGCT GATGAAAAGT TGGTCATGGA CACCTGGCAA 
CAGGCTTCTT TAGCAGTATC TCACATGGTG GCTGCAGAAA TTCATCAGAG GCTCATGGAA 
GAAGAAAAAG AAAACCAGCC AGCAGACCCC AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT 
AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA AAACAGGAAG ACAAAAAACT CAAAGGTAAA 
TCACCACCTA TTGCAGAAGC AACTCCTGTC ATAGTAACAA CAGAGGAAAT TGCTGAAATC 
GAAAGGAAAA ATGAACTGAG GCTCAAAATA AAAGAAGAAC ACCTTGCTGC CTTGCAATTT 
GAAGAAACAG CCACACAGTT TCGACTTGAA CTGATAAAGA CAAAAGCATT GGCTCTTCTT 
GAAGATTTAG TAACAAAGGT GGTTGATGTA TATAAACTCA TGGAAAAATG GCTTGGTGAG 
AGATATTTGA ATGAAATGGC CAGTGTAGAA AAATTAACTG ACATAGCTCG CTATCACATT 
GAAACATCTA CAAAAATTCA GAATGAACTT TATTTAAACC AAGAAGACTT CTTCATTAAT 
GGCAATGTAA AAGTCTTTCC AGATCCTCCC CCACCGATAC ATCCTCCACC TGTAGAAAAG 
GAAGAAGATG GCACCCTGAC CATCGAACAG CTTGACAATC TTCGGGATCA GTTCTTAGAT 
ATGGCACCTA AAGGCAGGAA TCCTTCAGAT GATGTGAATA GAGTCACCGA AAAGCAGAAA 
AGGCCACAGA AGCTTGACCA TTACCTTCCA AAAAGAAGCC ATCAATTTTT GAAATTTTGG 
CTTAAAAAAT ATTCATGGCT TATATATGAT GAGCTATTAA ATCTTATGTT CTGCGCCCTG 
TGTCGTAAGC ATGGAGTGAA GTCAGGGGGA AGTCAAGTGA ATTTCTTTTA TGGTACTGAC 
AACTTTAGGA CTCAATTTCT CGATGCACAT CAACTCAGCG AGGCTCATGC CAAGGCTTCA 
TTAATGGAAG CAACAAGTAG CTCTCCAGTG AACAGAGCCA CCACCAAACT AATGATGAGG 
ACCATGAGCA AAGTAACCCT CGGGAGAGTA GAGAACTTAT TCAGATCATG CCATGCTATT 
GCCAAGACCG GACGTACCCT CAAAGATTTT ATTTGGATGT GCAAATTGGA TGATATGAAA 
GGTGTTGATA TTGGCCCAGT ATTCAGAACT AAAAACTCAG CAAGAATGTT TACATACTTT 
ATTGCTGAAG TAGAACGAAA AAATCTAAGA GAGAAACTAG AAAAAAGTAA ATTTTTTTCT 
GTCATCAGTG ATGGAATCAC AGACAGTTTA ATCAAAGAAG TCGAACTTGT TTATGTGCAG 
TTTGCACATG CAGGAAAAGT GCACTGTCAA TTTGTTGGGG TACAGACAGT AGAAAGAAAT 
GATCCTCTGG CAATAAAAAA TGCCACTGAG AAAACTCTAG AGATAAATCT TCAACTTAGA 
CTGTCGAGCC AAGACTGGGC AAAGAAGCTG GTTGGTTTTG GAAGTGATGG TATCCATGGC 
ATAGAGGGAG AGAACAATGA GGTGGCCCTG CTACTACGAG AAATACAACC ATGTGTACAG 
ACTGTATATT GCTTTGCACA TCACCTTGAA CTATCTTACA AAGTGCTGTT CCAGAACATT 
CCTCTGTACA ATGATGTCAA AGATCTCCTT CACAGCATTT ATCACTTTTA TCACAATTCT 
CCTCTTCATA AGAGTGCTCT GAGAACTGCT TTCAAAGGCC TTCACCTTCG ACCTGTGATG 
CCTTCTCAAA TAGGAGACAG GAAGTGGCTT CATGGATTAC AGGCAGCCCT CCAGAACTTT 
CTCAAGGGAT ATCCTGCAAT TGTTCGGCAA CTGTACTCAG CAGGCGAAGG CAAAAGTGAC 
ACCAGTCAAA AGAAATCTAA AGAACTTCTG GAGTCCCTTC TCCAAGCTGA CATTGTTAAA 
TTTGCCCACT TCTTGTTGGA TGTCATTAAT GTCCTTAGCA TTCTGTCTGG TGTCAGTAAG 
AATAGAAATT CCTCAATTGC TGACATATTT GCCACTTTAG AGTCAATGCT GGAAATGCTC 
CAAAGGTATC AAACAAGACT AGGGCCAAAA GAACGCATGA TGGATTCAGC CACACACTTT 
CATGGTAACT GCCTCAGAGG GAAAGAAAAC ATTTCTGCTG TGAGAAACAT TGTTTTAACA 
CATCTTATCA AGAGGCTTCA AGGCTGTTTC AGAGATGCCA GCCAAAATGT GGTGAGGGCT 
ACCATGATTG GAAGTTTTAA ATTATGGCCC ACAAAGATAA ATCAAGAATT TGGAGAAAAA 
GAGGTATCCA TCCTGACTGC ACATTATGAA CCAGTATTAG AAGCTGCCAA TGTGAAACTT 
AGTGAAGTGG ATGCTGAATG GAGCATGTTG AAATTAGAGA TTTATGCCAG ATTTCCGAAC 
ATTCAAAAAT TGACCTGGGA TTTTGTGAAT TCCATTTACT TTCACAAGTA TCCAAATATC 
CTGACACTGG TCGACCTAGT GTTGACCCTT CCTGCAAGTT CAGCTGAAGC AGAACGTGGC 
TTTAGTCAGA TGAAATGGAC CAAGTCACAC ATGCATGCAA AAATCAAGAC TGAAATTATG 
ACGGATGTCC TAATTATTCA GTTGAATTCT CCAGACATTA ATAATTTTGA CCCTAGGAAA 
GCTATCCATT TATGGAATAC AAGAACACTG TCTTCAACTG GTGACACAAG ACCTAATTCA 
GATTGCTCAT CAAACTCAGA AAGCCATTAT GAAAGTGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007959537.1
CDS	107..6628
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGFLLGEVLYKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQPLTNLRLQHMKSETFRERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIKKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVANEIKKFEALIKKDLQAKESASKTTLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYKKHYSICAEILDQIVDLSTKVADYRTLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLIELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPMRILSIDTLVQEAIQAFHDNEKVSEILPIQKKDEEDALPVLQEEIKESQASDPQNVFSAGPVSNEGLPETEGETILSANADKTPKAEEIKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINEIPVNQHWILDGFPMTLNQAQLLEEALTGYNRNLTELERKKAQKSTLAIDPATSKEVPLPSPAFDFVVLLDVSDTSSMSRMKDIIAEELSYKTAHEDISQRVAAENQDKGGDQNLRDQIQHRIIGFLDNWPLLKQWFSEPENILIKINAEIDKESLCEKVKEILTTEIVKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHLHHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINENWLQDSLGMTMNHFFSLMQAELNRFQDTKRLLQDYYRGMESKIPIEDNKRFTQIPLVQLDNKDNSESQLRIPLFPRISISLETVTPKPKTKSILKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKLKGKSPPIAEATPVIVTTEEIAEIERKNELRLKIKEEHLAALQFEETATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASVEKLTDIARYHIETSTKIQNELYLNQEDFFINGNVKVFPDPPPPIHPPPVEKEEDGTLTIEQLDNLRDQFLDMAPKGRNPSDDVNRVTEKQKRPQKLDHYLPKRSHQFLKFWLKKYSWLIYDELLNLMFCALCRKHGVKSGGSQVNFFYGTDNFRTQFLDAHQLSEAHAKASLMEATSSSPVNRATTKLMMRTMSKVTLGRVENLFRSCHAIAKTGRTLKDFIWMCKLDDMKGVDIGPVFRTKNSARMFTYFIAEVERKNLREKLEKSKFFSVISDGITDSLIKEVELVYVQFAHAGKVHCQFVGVQTVERNDPLAIKNATEKTLEINLQLRLSSQDWAKKLVGFGSDGIHGIEGENNEVALLLREIQPCVQTVYCFAHHLELSYKVLFQNIPLYNDVKDLLHSIYHFYHNSPLHKSALRTAFKGLHLRPVMPSQIGDRKWLHGLQAALQNFLKGYPAIVRQLYSAGEGKSDTSQKKSKELLESLLQADIVKFAHFLLDVINVLSILSGVSKNRNSSIADIFATLESMLEMLQRYQTRLGPKERMMDSATHFHGNCLRGKENISAVRNIVLTHLIKRLQGCFRDASQNVVRATMIGSFKLWPTKINQEFGEKEVSILTAHYEPVLEAANVKLSEVDAEWSMLKLEIYARFPNIQKLTWDFVNSIYFHKYPNILTLVDLVLTLPASSAEAERGFSQMKWTKSHMHAKIKTEIMTDVLIIQLNSPDINNFDPRKAIHLWNTRTLSSTGDTRPNSDCSSNSESHYESD

Target ORF information:

RefSeq Version	XM_007961346.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007961346.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG CAAGTGATCC ACAAAATGTA 
TTTTCAGCTG GTCCAGTTTC AAATGAAGGA TTACCAGAAA CAGAAGGTGA AACGATACTA 
AGTGCTAATG CTGATAAAAC ACCAAAAGCT GAAGAAATCA AATCCAGTGA CAGTTTCTTA 
AAACTCACTA CGCGTGCTCA ACTTGGTGCA AAATCAGAAC AGTTGCTGAA GAAAGGAAAG 
AGCATTCCTG ACGTGCTGCT TGTTGACATC ATGGTAAATG CTATTAACGA GATACCTGTG 
AATCAGCACT GGATCCTAGA TGGTTTTCCA ATGACTTTAA ATCAAGCACA GCTTCTGGAA 
GAAGCTCTTA CAGGCTACAA TAGAAACCTC ACAGAACTGG AGAGAAAAAA AGCACAAAAA 
TCCACATTGG CTATTGATCC TGCGACTTCC AAAGAAGTAC CTCTTCCCTC TCCTGCATTT 
GATTTTGTCG TATTATTAGA CGTTTCAGAT ACTTCCTCAA TGAGTCGCAT GAAGGATATA 
ATAGCTGAAG AATTGTCCTA TAAAACTGCT CACGAAGACA TCAGTCAACG TGTAGCTGCT 
GAAAATCAAG ATAAGGGTGG AGACCAAAAT TTAAGAGACC AGATACAACA TAGAATTATA 
GGCTTCTTGG ACAACTGGCC TTTATTGAAG CAATGGTTTT CAGAGCCAGA AAATATTTTG 
ATAAAAATCA ATGCTGAAAT AGATAAGGAA TCTTTATGCG AAAAAGTAAA AGAAATTCTT 
ACGACTGAAA TAGTGAAAAA AAAGAATAAA GTTGAGAAGA AATTAGAAGA AAAGGAAGCT 
GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG CTTCCACTTC CTACACCTCC TCCTGCTCCT 
CCTCCTGAAC CAGAAAAAGA GAAGGAAATT CATCTTCATC ATGTGCCTTC AAAAACTCCT 
ACTGCAAAAG GAAAACCTCA ATCAGAAGCC CCGCATGGTA AGCAAGAATC TCTTCAGGAA 
GGAAAAGGGA AGAAAGGTGA AACGGCACTC AAAAGAAAAG GTTCTCCTAA AGGAAAATCA 
TCAGGAGGAA AAGTACCAGT AAAGAAATCA CCTGCTGACT CTACAGACAC ATCACCTGTT 
GCAATAGTGC CGCAGCCACC TAAGCCAGGA TCAGAAGAAT GGGTCTATGT GAATGAACCA 
GTTCCTGAGG AAATGCCTTC GTTTTTAGTA CCTTACTGGG AACTAATAGA AAATTCCTAT 
ATAAACACCA TCAAAACAGT ACTCAGGCAT CTGAGGGAAG ACCAGCATAC TGTGCTTGCT 
TACTTATATG AGATTAGAAC AAGTTTCCAG GAGTTTCTAA AGCGTCCAGA TCACAAGCAA 
GATTTTGTAG CTCAATGGCA GGCTGATTTC AATTCCCTTC CTGATGACCT GTGGGATGAT 
GAGGAAACAA AGGCTGAACT ACACCAACGA GTGAATGATC TGCGAGACCG CCTGTGGGAC 
ATTTGTGACG CACGGAAGGA AGAGGCAGAG CAGGAGCGGC TTGACATCAT TAATGAGAAC 
TGGTTACAGG ACTCTCTTGG AATGACAATG AACCATTTCT TTTCCCTGAT GCAGGCAGAA 
CTGAACCGTT TCCAAGATAC GAAGAGACTC CTTCAAGATT ATTACCGGGG AATGGAAAGT 
AAAATCCCAA TAGAGGACAA TAAGAGATTT ACTCAAATCC CTTTGGTCCA GCTGGATAAT 
AAAGACAATT CTGAAAGCCA GCTTAGAATT CCTCTATTTC CTCGAATATC CATTTCTCTG 
GAAACAGTTA CGCCCAAACC AAAAACAAAA TCAATACTGA AGGGCAAGAT GGATAACTCC 
CTAGAAAACG TTGAGTCCAA CTTTGAGGCT GATGAAAAGT TGGTCATGGA CACCTGGCAA 
CAGGCTTCTT TAGCAGTATC TCACATGGTG GCTGCAGAAA TTCATCAGAG GCTCATGGAA 
GAAGAAAAAG AAAACCAGCC AGCAGACCCC AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT 
AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA AAACAGGAAG ACAAAAAACT CAAAGGTAAA 
TCACCACCTA TTGCAGAAGC AACTCCTGTC ATAGTAACAA CAGAGGAAAT TGCTGAAATC 
GAAAGGAAAA ATGAACTGAG GCTCAAAATA AAAGAAGAAC ACCTTGCTGC CTTGCAATTT 
GAAGAAACAG CCACACAGTT TCGACTTGAA CTGATAAAGA CAAAAGCATT GGCTCTTCTT 
GAAGATTTAG TAACAAAGGT GGTTGATGTA TATAAACTCA TGGAAAAATG GCTTGGTGAG 
AGATATTTGA ATGAAATGGC CAGTGTAGAA AAATTAACTG ACATAGCTCG CTATCACATT 
GAAACATCTA CAAAAATTCA GAATGAACTT TATTTAAACC AAGAAGACTT CTTCATTAAT 
GGCAATGTAA AAGTCTTTCC AGATCCTCCC CCACCGATAC ATCCTCCACC TGTAGAAAAG 
GAAGAAGATG GCACCCTGAC CATCGAACAG CTTGACAATC TTCGGGATCA GTTCTTAGAT 
ATGGCACCTA AAGGCAGGAA TCCTTCAGAT GATGTGAATA GAGTCACCGA AAAGCAGAAA 
AGGCCACAGA AGCTTGACCA TTACCTTCCA AAAAGAAGCC ATCAATTTTT GAAATTTTGG 
CTTAAAAAAT ATTCATGGCT TATATATGAT GAGCTATTAA ATCTTATGTT CTGCGCCCTG 
TGTCGTAAGC ATGGAGTGAA GTCAGGGGGA AGTCAAGTGA ATTTCTTTTA TGGTACTGAC 
AACTTTAGGA CTCAATTTCT CGATGCACAT CAACTCAGCG AGGCTCATGC CAAGGCTTCA 
TTAATGGAAG CAACAAGTAG CTCTCCAGTG AACAGAGCCA CCACCAAACT AATGATGAGG 
ACCATGAGCA AAGTAACCCT CGGGAGAGTA GAGAACTTAT TCAGATCATG CCATGCTATT 
GCCAAGACCG GACGTACCCT CAAAGATTTT ATTTGGATGT GCAAATTGGA TGATATGAAA 
GGTGTTGATA TTGGCCCAGT ATTCAGAACT AAAAACTCAG CAAGAATGTT TACATACTTT 
ATTGCTGAAG TAGAACGAAA AAATCTAAGA GAGAAACTAG AAAAAAGTAA ATTTTTTTCT 
GTCATCAGTG ATGGAATCAC AGACAGTTTA ATCAAAGAAG TCGAACTTGT TTATGTGCAG 
TTTGCACATG CAGGAAAAGT GCACTGTCAA TTTGTTGGGG TACAGACAGT AGAAAGAAAT 
GATCCTCTGG CAATAAAAAA TGCCACTGAG AAAACTCTAG AGATAAATCT TCAACTTAGA 
CTGTCGAGCC AAGACTGGGC AAAGAAGCTG GTTGGTTTTG GAAGTGATGG TATCCATGGC 
ATAGAGGGAG AGAACAATGA GGTGGCCCTG CTACTACGAG AAATACAACC ATGTGTACAG 
ACTGTATATT GCTTTGCACA TCACCTTGAA CTATCTTACA AAGTGCTGTT CCAGAACATT 
CCTCTGTACA ATGATGTCAA AGATCTCCTT CACAGCATTT ATCACTTTTA TCACAATTCT 
CCTCTTCATA AGAGTGCTCT GAGAACTGCT TTCAAAGGCC TTCACCTTCG ACCTGTGATG 
CCTTCTCAAA TAGGAGACAG GAAGTGGCTT CATGGATTAC AGGCAGCCCT CCAGAACTTT 
CTCAAGGGAT ATCCTGCAAT TGTTCGGCAA CTGTACTCAG CAGGCGAAGG CAAAAGTGAC 
ACCAGTCAAA AGAAATCTAA AGAACTTCTG GAGTCCCTTC TCCAAGCTGA CATTGTTAAA 
TTTGCCCACT TCTTGTTGGA TGTCATTAAT GTCCTTAGCA TTCTGTCTGG TGTCAGTAAG 
AATAGAAATT CCTCAATTGC TGACATATTT GCCACTTTAG AGTCAATGCT GGAAATGCTC 
CAAAGGTATC AAACAAGACT AGGGCCAAAA GAACGCATGA TGGATTCAGC CACACACTTT 
CATGGTAACT GCCTCAGAGG GAAAGAAAAC ATTTCTGCTG TGAGAAACAT TGTTTTAACA 
CATCTTATCA AGAGGCTTCA AGGCTGTTTC AGAGATGCCA GCCAAAATGT GGTGAGGGCT 
ACCATGATTG GAAGTTTTAA ATTATGGCCC ACAAAGATAA ATCAAGAATT TGGAGAAAAA 
GAGGTATCCA TCCTGACTGC ACATTATGAA CCAGTATTAG AAGCTGCCAA TGTGAAACTT 
AGTGAAGTGG ATGCTGAATG GAGCATGTTG AAATTAGAGA TTTATGCCAG ATTTCCGAAC 
ATTCAAAAAT TGACCTGGGA TTTTGTGAAT TCCATTTACT TTCACAAGTA TCCAAATATC 
CTGACACTGG TCGACCTAGT GTTGACCCTT CCTGCAAGTT CAGCTGAAGC AGAACGTGGC 
TTTAGTCAGA TGAAATGGAC CAAGTCACAC ATGCATGCAA AAATCAAGAC TGAAATTATG 
ACGGATGTCC TAATTATTCA GTTGAATTCT CCAGACATTA ATAATTTTGA CCCTAGGAAA 
GCTATCCATT TATGGAATAC AAGAACACTG TCTTCAACTG GTGACACAAG ACCTAATTCA 
GATTGCTCAT CAAACTCAGA AAGCCATTAT GAAAGTGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_007961347.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007959538.1 Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.

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CloneID	OCh83458
Clone ID Related Accession (Same CDS sequence)	XM_007961347.1
Accession Version	XM_007961347.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	sperm flagellar protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735239.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAAAA TGTATTTTCA 
GCTGGTCCAG TTTCAAATGA AGGATTACCA GAAACAGAAG GTGAAACGAT ACTAAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA ATCAAATCCA GTGACAGTTT CTTAAAACTC 
ACTACGCGTG CTCAACTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGACGTGC TGCTTGTTGA CATCATGGTA AATGCTATTA ACGAGATACC TGTGAATCAG 
CACTGGATCC TAGATGGTTT TCCAATGACT TTAAATCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT ACAATAGAAA CCTCACAGAA CTGGAGAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA GTACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCGTATTAT TAGACGTTTC AGATACTTCC TCAATGAGTC GCATGAAGGA TATAATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GACATCAGTC AACGTGTAGC TGCTGAAAAT 
CAAGATAAGG GTGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GAAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGCGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGTGA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCTT CATCATGTGC CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAAACGGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ACACATCACC TGTTGCAATA 
GTGCCGCAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTCGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CAGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAATTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACACCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GACGCACGGA AGGAAGAGGC AGAGCAGGAG CGGCTTGACA TCATTAATGA GAACTGGTTA 
CAGGACTCTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAACTGAAC 
CGTTTCCAAG ATACGAAGAG ACTCCTTCAA GATTATTACC GGGGAATGGA AAGTAAAATC 
CCAATAGAGG ACAATAAGAG ATTTACTCAA ATCCCTTTGG TCCAGCTGGA TAATAAAGAC 
AATTCTGAAA GCCAGCTTAG AATTCCTCTA TTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACGCCCA AACCAAAAAC AAAATCAATA CTGAAGGGCA AGATGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCTGATGAA AAGTTGGTCA TGGACACCTG GCAACAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCA GAAATTCATC AGAGGCTCAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACTCAAAGG TAAATCACCA 
CCTATTGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCGAAAGG 
AAAAATGAAC TGAGGCTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ACAGCCACAC AGTTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGATAT 
TTGAATGAAA TGGCCAGTGT AGAAAAATTA ACTGACATAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AACCAAGAAG ACTTCTTCAT TAATGGCAAT 
GTAAAAGTCT TTCCAGATCC TCCCCCACCG ATACATCCTC CACCTGTAGA AAAGGAAGAA 
GATGGCACCC TGACCATCGA ACAGCTTGAC AATCTTCGGG ATCAGTTCTT AGATATGGCA 
CCTAAAGGCA GGAATCCTTC AGATGATGTG AATAGAGTCA CCGAAAAGCA GAAAAGGCCA 
CAGAAGCTTG ACCATTACCT TCCAAAAAGA AGCCATCAAT TTTTGAAATT TTGGCTTAAA 
AAATATTCAT GGCTTATATA TGATGAGCTA TTAAATCTTA TGTTCTGCGC CCTGTGTCGT 
AAGCATGGAG TGAAGTCAGG GGGAAGTCAA GTGAATTTCT TTTATGGTAC TGACAACTTT 
AGGACTCAAT TTCTCGATGC ACATCAACTC AGCGAGGCTC ATGCCAAGGC TTCATTAATG 
GAAGCAACAA GTAGCTCTCC AGTGAACAGA GCCACCACCA AACTAATGAT GAGGACCATG 
AGCAAAGTAA CCCTCGGGAG AGTAGAGAAC TTATTCAGAT CATGCCATGC TATTGCCAAG 
ACCGGACGTA CCCTCAAAGA TTTTATTTGG ATGTGCAAAT TGGATGATAT GAAAGGTGTT 
GATATTGGCC CAGTATTCAG AACTAAAAAC TCAGCAAGAA TGTTTACATA CTTTATTGCT 
GAAGTAGAAC GAAAAAATCT AAGAGAGAAA CTAGAAAAAA GTAAATTTTT TTCTGTCATC 
AGTGATGGAA TCACAGACAG TTTAATCAAA GAAGTCGAAC TTGTTTATGT GCAGTTTGCA 
CATGCAGGAA AAGTGCACTG TCAATTTGTT GGGGTACAGA CAGTAGAAAG AAATGATCCT 
CTGGCAATAA AAAATGCCAC TGAGAAAACT CTAGAGATAA ATCTTCAACT TAGACTGTCG 
AGCCAAGACT GGGCAAAGAA GCTGGTTGGT TTTGGAAGTG ATGGTATCCA TGGCATAGAG 
GGAGAGAACA ATGAGGTGGC CCTGCTACTA CGAGAAATAC AACCATGTGT ACAGACTGTA 
TATTGCTTTG CACATCACCT TGAACTATCT TACAAAGTGC TGTTCCAGAA CATTCCTCTG 
TACAATGATG TCAAAGATCT CCTTCACAGC ATTTATCACT TTTATCACAA TTCTCCTCTT 
CATAAGAGTG CTCTGAGAAC TGCTTTCAAA GGCCTTCACC TTCGACCTGT GATGCCTTCT 
CAAATAGGAG ACAGGAAGTG GCTTCATGGA TTACAGGCAG CCCTCCAGAA CTTTCTCAAG 
GGATATCCTG CAATTGTTCG GCAACTGTAC TCAGCAGGCG AAGGCAAAAG TGACACCAGT 
CAAAAGAAAT CTAAAGAACT TCTGGAGTCC CTTCTCCAAG CTGACATTGT TAAATTTGCC 
CACTTCTTGT TGGATGTCAT TAATGTCCTT AGCATTCTGT CTGGTGTCAG TAAGAATAGA 
AATTCCTCAA TTGCTGACAT ATTTGCCACT TTAGAGTCAA TGCTGGAAAT GCTCCAAAGG 
TATCAAACAA GACTAGGGCC AAAAGAACGC ATGATGGATT CAGCCACACA CTTTCATGGT 
AACTGCCTCA GAGGGAAAGA AAACATTTCT GCTGTGAGAA ACATTGTTTT AACACATCTT 
ATCAAGAGGC TTCAAGGCTG TTTCAGAGAT GCCAGCCAAA ATGTGGTGAG GGCTACCATG 
ATTGGAAGTT TTAAATTATG GCCCACAAAG ATAAATCAAG AATTTGGAGA AAAAGAGGTA 
TCCATCCTGA CTGCACATTA TGAACCAGTA TTAGAAGCTG CCAATGTGAA ACTTAGTGAA 
GTGGATGCTG AATGGAGCAT GTTGAAATTA GAGATTTATG CCAGATTTCC GAACATTCAA 
AAATTGACCT GGGATTTTGT GAATTCCATT TACTTTCACA AGTATCCAAA TATCCTGACA 
CTGGTCGACC TAGTGTTGAC CCTTCCTGCA AGTTCAGCTG AAGCAGAACG TGGCTTTAGT 
CAGATGAAAT GGACCAAGTC ACACATGCAT GCAAAAATCA AGACTGAAAT TATGACGGAT 
GTCCTAATTA TTCAGTTGAA TTCTCCAGAC ATTAATAATT TTGACCCTAG GAAAGCTATC 
CATTTATGGA ATACAAGAAC ACTGTCTTCA ACTGGTGACA CAAGACCTAA TTCAGATTGC 
TCATCAAACT CAGAAAGCCA TTATGAAAGT GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007959538.1
CDS	107..6622
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGFLLGEVLYKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQPLTNLRLQHMKSETFRERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIKKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVANEIKKFEALIKKDLQAKESASKTTLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYKKHYSICAEILDQIVDLSTKVADYRTLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLIELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPMRILSIDTLVQEAIQAFHDNEKVSEILPIQKKDEEDALPVLQEEIKESQDPQNVFSAGPVSNEGLPETEGETILSANADKTPKAEEIKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINEIPVNQHWILDGFPMTLNQAQLLEEALTGYNRNLTELERKKAQKSTLAIDPATSKEVPLPSPAFDFVVLLDVSDTSSMSRMKDIIAEELSYKTAHEDISQRVAAENQDKGGDQNLRDQIQHRIIGFLDNWPLLKQWFSEPENILIKINAEIDKESLCEKVKEILTTEIVKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHLHHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINENWLQDSLGMTMNHFFSLMQAELNRFQDTKRLLQDYYRGMESKIPIEDNKRFTQIPLVQLDNKDNSESQLRIPLFPRISISLETVTPKPKTKSILKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKLKGKSPPIAEATPVIVTTEEIAEIERKNELRLKIKEEHLAALQFEETATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASVEKLTDIARYHIETSTKIQNELYLNQEDFFINGNVKVFPDPPPPIHPPPVEKEEDGTLTIEQLDNLRDQFLDMAPKGRNPSDDVNRVTEKQKRPQKLDHYLPKRSHQFLKFWLKKYSWLIYDELLNLMFCALCRKHGVKSGGSQVNFFYGTDNFRTQFLDAHQLSEAHAKASLMEATSSSPVNRATTKLMMRTMSKVTLGRVENLFRSCHAIAKTGRTLKDFIWMCKLDDMKGVDIGPVFRTKNSARMFTYFIAEVERKNLREKLEKSKFFSVISDGITDSLIKEVELVYVQFAHAGKVHCQFVGVQTVERNDPLAIKNATEKTLEINLQLRLSSQDWAKKLVGFGSDGIHGIEGENNEVALLLREIQPCVQTVYCFAHHLELSYKVLFQNIPLYNDVKDLLHSIYHFYHNSPLHKSALRTAFKGLHLRPVMPSQIGDRKWLHGLQAALQNFLKGYPAIVRQLYSAGEGKSDTSQKKSKELLESLLQADIVKFAHFLLDVINVLSILSGVSKNRNSSIADIFATLESMLEMLQRYQTRLGPKERMMDSATHFHGNCLRGKENISAVRNIVLTHLIKRLQGCFRDASQNVVRATMIGSFKLWPTKINQEFGEKEVSILTAHYEPVLEAANVKLSEVDAEWSMLKLEIYARFPNIQKLTWDFVNSIYFHKYPNILTLVDLVLTLPASSAEAERGFSQMKWTKSHMHAKIKTEIMTDVLIIQLNSPDINNFDPRKAIHLWNTRTLSSTGDTRPNSDCSSNSESHYESD

Target ORF information:

RefSeq Version	XM_007961347.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007961347.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAAAA TGTATTTTCA 
GCTGGTCCAG TTTCAAATGA AGGATTACCA GAAACAGAAG GTGAAACGAT ACTAAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA ATCAAATCCA GTGACAGTTT CTTAAAACTC 
ACTACGCGTG CTCAACTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGACGTGC TGCTTGTTGA CATCATGGTA AATGCTATTA ACGAGATACC TGTGAATCAG 
CACTGGATCC TAGATGGTTT TCCAATGACT TTAAATCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT ACAATAGAAA CCTCACAGAA CTGGAGAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA GTACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCGTATTAT TAGACGTTTC AGATACTTCC TCAATGAGTC GCATGAAGGA TATAATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GACATCAGTC AACGTGTAGC TGCTGAAAAT 
CAAGATAAGG GTGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GAAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGCGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGTGA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCTT CATCATGTGC CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAAACGGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ACACATCACC TGTTGCAATA 
GTGCCGCAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTCGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CAGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAATTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACACCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GACGCACGGA AGGAAGAGGC AGAGCAGGAG CGGCTTGACA TCATTAATGA GAACTGGTTA 
CAGGACTCTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAACTGAAC 
CGTTTCCAAG ATACGAAGAG ACTCCTTCAA GATTATTACC GGGGAATGGA AAGTAAAATC 
CCAATAGAGG ACAATAAGAG ATTTACTCAA ATCCCTTTGG TCCAGCTGGA TAATAAAGAC 
AATTCTGAAA GCCAGCTTAG AATTCCTCTA TTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACGCCCA AACCAAAAAC AAAATCAATA CTGAAGGGCA AGATGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCTGATGAA AAGTTGGTCA TGGACACCTG GCAACAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCA GAAATTCATC AGAGGCTCAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACTCAAAGG TAAATCACCA 
CCTATTGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCGAAAGG 
AAAAATGAAC TGAGGCTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ACAGCCACAC AGTTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGATAT 
TTGAATGAAA TGGCCAGTGT AGAAAAATTA ACTGACATAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AACCAAGAAG ACTTCTTCAT TAATGGCAAT 
GTAAAAGTCT TTCCAGATCC TCCCCCACCG ATACATCCTC CACCTGTAGA AAAGGAAGAA 
GATGGCACCC TGACCATCGA ACAGCTTGAC AATCTTCGGG ATCAGTTCTT AGATATGGCA 
CCTAAAGGCA GGAATCCTTC AGATGATGTG AATAGAGTCA CCGAAAAGCA GAAAAGGCCA 
CAGAAGCTTG ACCATTACCT TCCAAAAAGA AGCCATCAAT TTTTGAAATT TTGGCTTAAA 
AAATATTCAT GGCTTATATA TGATGAGCTA TTAAATCTTA TGTTCTGCGC CCTGTGTCGT 
AAGCATGGAG TGAAGTCAGG GGGAAGTCAA GTGAATTTCT TTTATGGTAC TGACAACTTT 
AGGACTCAAT TTCTCGATGC ACATCAACTC AGCGAGGCTC ATGCCAAGGC TTCATTAATG 
GAAGCAACAA GTAGCTCTCC AGTGAACAGA GCCACCACCA AACTAATGAT GAGGACCATG 
AGCAAAGTAA CCCTCGGGAG AGTAGAGAAC TTATTCAGAT CATGCCATGC TATTGCCAAG 
ACCGGACGTA CCCTCAAAGA TTTTATTTGG ATGTGCAAAT TGGATGATAT GAAAGGTGTT 
GATATTGGCC CAGTATTCAG AACTAAAAAC TCAGCAAGAA TGTTTACATA CTTTATTGCT 
GAAGTAGAAC GAAAAAATCT AAGAGAGAAA CTAGAAAAAA GTAAATTTTT TTCTGTCATC 
AGTGATGGAA TCACAGACAG TTTAATCAAA GAAGTCGAAC TTGTTTATGT GCAGTTTGCA 
CATGCAGGAA AAGTGCACTG TCAATTTGTT GGGGTACAGA CAGTAGAAAG AAATGATCCT 
CTGGCAATAA AAAATGCCAC TGAGAAAACT CTAGAGATAA ATCTTCAACT TAGACTGTCG 
AGCCAAGACT GGGCAAAGAA GCTGGTTGGT TTTGGAAGTG ATGGTATCCA TGGCATAGAG 
GGAGAGAACA ATGAGGTGGC CCTGCTACTA CGAGAAATAC AACCATGTGT ACAGACTGTA 
TATTGCTTTG CACATCACCT TGAACTATCT TACAAAGTGC TGTTCCAGAA CATTCCTCTG 
TACAATGATG TCAAAGATCT CCTTCACAGC ATTTATCACT TTTATCACAA TTCTCCTCTT 
CATAAGAGTG CTCTGAGAAC TGCTTTCAAA GGCCTTCACC TTCGACCTGT GATGCCTTCT 
CAAATAGGAG ACAGGAAGTG GCTTCATGGA TTACAGGCAG CCCTCCAGAA CTTTCTCAAG 
GGATATCCTG CAATTGTTCG GCAACTGTAC TCAGCAGGCG AAGGCAAAAG TGACACCAGT 
CAAAAGAAAT CTAAAGAACT TCTGGAGTCC CTTCTCCAAG CTGACATTGT TAAATTTGCC 
CACTTCTTGT TGGATGTCAT TAATGTCCTT AGCATTCTGT CTGGTGTCAG TAAGAATAGA 
AATTCCTCAA TTGCTGACAT ATTTGCCACT TTAGAGTCAA TGCTGGAAAT GCTCCAAAGG 
TATCAAACAA GACTAGGGCC AAAAGAACGC ATGATGGATT CAGCCACACA CTTTCATGGT 
AACTGCCTCA GAGGGAAAGA AAACATTTCT GCTGTGAGAA ACATTGTTTT AACACATCTT 
ATCAAGAGGC TTCAAGGCTG TTTCAGAGAT GCCAGCCAAA ATGTGGTGAG GGCTACCATG 
ATTGGAAGTT TTAAATTATG GCCCACAAAG ATAAATCAAG AATTTGGAGA AAAAGAGGTA 
TCCATCCTGA CTGCACATTA TGAACCAGTA TTAGAAGCTG CCAATGTGAA ACTTAGTGAA 
GTGGATGCTG AATGGAGCAT GTTGAAATTA GAGATTTATG CCAGATTTCC GAACATTCAA 
AAATTGACCT GGGATTTTGT GAATTCCATT TACTTTCACA AGTATCCAAA TATCCTGACA 
CTGGTCGACC TAGTGTTGAC CCTTCCTGCA AGTTCAGCTG AAGCAGAACG TGGCTTTAGT 
CAGATGAAAT GGACCAAGTC ACACATGCAT GCAAAAATCA AGACTGAAAT TATGACGGAT 
GTCCTAATTA TTCAGTTGAA TTCTCCAGAC ATTAATAATT TTGACCCTAG GAAAGCTATC 
CATTTATGGA ATACAAGAAC ACTGTCTTCA ACTGGTGACA CAAGACCTAA TTCAGATTGC 
TCATCAAACT CAGAAAGCCA TTATGAAAGT GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_007961348.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007959539.1 Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.

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CloneID	OCh83459
Clone ID Related Accession (Same CDS sequence)	XM_007961348.1
Accession Version	XM_007961348.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	sperm flagellar protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735239.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG CAAGTGATCC ACAAAATGTA 
TTTTCAGCTG GTCCAGTTTC AAATGAAGGA TTACCAGAAA CAGAAGGTGC TAATGCTGAT 
AAAACACCAA AAGCTGAAGA AATCAAATCC AGTGACAGTT TCTTAAAACT CACTACGCGT 
GCTCAACTTG GTGCAAAATC AGAACAGTTG CTGAAGAAAG GAAAGAGCAT TCCTGACGTG 
CTGCTTGTTG ACATCATGGT AAATGCTATT AACGAGATAC CTGTGAATCA GCACTGGATC 
CTAGATGGTT TTCCAATGAC TTTAAATCAA GCACAGCTTC TGGAAGAAGC TCTTACAGGC 
TACAATAGAA ACCTCACAGA ACTGGAGAGA AAAAAAGCAC AAAAATCCAC ATTGGCTATT 
GATCCTGCGA CTTCCAAAGA AGTACCTCTT CCCTCTCCTG CATTTGATTT TGTCGTATTA 
TTAGACGTTT CAGATACTTC CTCAATGAGT CGCATGAAGG ATATAATAGC TGAAGAATTG 
TCCTATAAAA CTGCTCACGA AGACATCAGT CAACGTGTAG CTGCTGAAAA TCAAGATAAG 
GGTGGAGACC AAAATTTAAG AGACCAGATA CAACATAGAA TTATAGGCTT CTTGGACAAC 
TGGCCTTTAT TGAAGCAATG GTTTTCAGAG CCAGAAAATA TTTTGATAAA AATCAATGCT 
GAAATAGATA AGGAATCTTT ATGCGAAAAA GTAAAAGAAA TTCTTACGAC TGAAATAGTG 
AAAAAAAAGA ATAAAGTTGA GAAGAAATTA GAAGAAAAGG AAGCTGAGAA AAAAGCAGCA 
GCTTCCCTGG CTGAGCTTCC ACTTCCTACA CCTCCTCCTG CTCCTCCTCC TGAACCAGAA 
AAAGAGAAGG AAATTCATCT TCATCATGTG CCTTCAAAAA CTCCTACTGC AAAAGGAAAA 
CCTCAATCAG AAGCCCCGCA TGGTAAGCAA GAATCTCTTC AGGAAGGAAA AGGGAAGAAA 
GGTGAAACGG CACTCAAAAG AAAAGGTTCT CCTAAAGGAA AATCATCAGG AGGAAAAGTA 
CCAGTAAAGA AATCACCTGC TGACTCTACA GACACATCAC CTGTTGCAAT AGTGCCGCAG 
CCACCTAAGC CAGGATCAGA AGAATGGGTC TATGTGAATG AACCAGTTCC TGAGGAAATG 
CCTTCGTTTT TAGTACCTTA CTGGGAACTA ATAGAAAATT CCTATATAAA CACCATCAAA 
ACAGTACTCA GGCATCTGAG GGAAGACCAG CATACTGTGC TTGCTTACTT ATATGAGATT 
AGAACAAGTT TCCAGGAGTT TCTAAAGCGT CCAGATCACA AGCAAGATTT TGTAGCTCAA 
TGGCAGGCTG ATTTCAATTC CCTTCCTGAT GACCTGTGGG ATGATGAGGA AACAAAGGCT 
GAACTACACC AACGAGTGAA TGATCTGCGA GACCGCCTGT GGGACATTTG TGACGCACGG 
AAGGAAGAGG CAGAGCAGGA GCGGCTTGAC ATCATTAATG AGAACTGGTT ACAGGACTCT 
CTTGGAATGA CAATGAACCA TTTCTTTTCC CTGATGCAGG CAGAACTGAA CCGTTTCCAA 
GATACGAAGA GACTCCTTCA AGATTATTAC CGGGGAATGG AAAGTAAAAT CCCAATAGAG 
GACAATAAGA GATTTACTCA AATCCCTTTG GTCCAGCTGG ATAATAAAGA CAATTCTGAA 
AGCCAGCTTA GAATTCCTCT ATTTCCTCGA ATATCCATTT CTCTGGAAAC AGTTACGCCC 
AAACCAAAAA CAAAATCAAT ACTGAAGGGC AAGATGGATA ACTCCCTAGA AAACGTTGAG 
TCCAACTTTG AGGCTGATGA AAAGTTGGTC ATGGACACCT GGCAACAGGC TTCTTTAGCA 
GTATCTCACA TGGTGGCTGC AGAAATTCAT CAGAGGCTCA TGGAAGAAGA AAAAGAAAAC 
CAGCCAGCAG ACCCCAAAGA AAAATCTCCT CAGATGGGTG CAAATAAAAA AGTCAAAAAG 
GAGCCACCCA AGAAAAAACA GGAAGACAAA AAACTCAAAG GTAAATCACC ACCTATTGCA 
GAAGCAACTC CTGTCATAGT AACAACAGAG GAAATTGCTG AAATCGAAAG GAAAAATGAA 
CTGAGGCTCA AAATAAAAGA AGAACACCTT GCTGCCTTGC AATTTGAAGA AACAGCCACA 
CAGTTTCGAC TTGAACTGAT AAAGACAAAA GCATTGGCTC TTCTTGAAGA TTTAGTAACA 
AAGGTGGTTG ATGTATATAA ACTCATGGAA AAATGGCTTG GTGAGAGATA TTTGAATGAA 
ATGGCCAGTG TAGAAAAATT AACTGACATA GCTCGCTATC ACATTGAAAC ATCTACAAAA 
ATTCAGAATG AACTTTATTT AAACCAAGAA GACTTCTTCA TTAATGGCAA TGTAAAAGTC 
TTTCCAGATC CTCCCCCACC GATACATCCT CCACCTGTAG AAAAGGAAGA AGATGGCACC 
CTGACCATCG AACAGCTTGA CAATCTTCGG GATCAGTTCT TAGATATGGC ACCTAAAGGC 
AGGAATCCTT CAGATGATGT GAATAGAGTC ACCGAAAAGC AGAAAAGGCC ACAGAAGCTT 
GACCATTACC TTCCAAAAAG AAGCCATCAA TTTTTGAAAT TTTGGCTTAA AAAATATTCA 
TGGCTTATAT ATGATGAGCT ATTAAATCTT ATGTTCTGCG CCCTGTGTCG TAAGCATGGA 
GTGAAGTCAG GGGGAAGTCA AGTGAATTTC TTTTATGGTA CTGACAACTT TAGGACTCAA 
TTTCTCGATG CACATCAACT CAGCGAGGCT CATGCCAAGG CTTCATTAAT GGAAGCAACA 
AGTAGCTCTC CAGTGAACAG AGCCACCACC AAACTAATGA TGAGGACCAT GAGCAAAGTA 
ACCCTCGGGA GAGTAGAGAA CTTATTCAGA TCATGCCATG CTATTGCCAA GACCGGACGT 
ACCCTCAAAG ATTTTATTTG GATGTGCAAA TTGGATGATA TGAAAGGTGT TGATATTGGC 
CCAGTATTCA GAACTAAAAA CTCAGCAAGA ATGTTTACAT ACTTTATTGC TGAAGTAGAA 
CGAAAAAATC TAAGAGAGAA ACTAGAAAAA AGTAAATTTT TTTCTGTCAT CAGTGATGGA 
ATCACAGACA GTTTAATCAA AGAAGTCGAA CTTGTTTATG TGCAGTTTGC ACATGCAGGA 
AAAGTGCACT GTCAATTTGT TGGGGTACAG ACAGTAGAAA GAAATGATCC TCTGGCAATA 
AAAAATGCCA CTGAGAAAAC TCTAGAGATA AATCTTCAAC TTAGACTGTC GAGCCAAGAC 
TGGGCAAAGA AGCTGGTTGG TTTTGGAAGT GATGGTATCC ATGGCATAGA GGGAGAGAAC 
AATGAGGTGG CCCTGCTACT ACGAGAAATA CAACCATGTG TACAGACTGT ATATTGCTTT 
GCACATCACC TTGAACTATC TTACAAAGTG CTGTTCCAGA ACATTCCTCT GTACAATGAT 
GTCAAAGATC TCCTTCACAG CATTTATCAC TTTTATCACA ATTCTCCTCT TCATAAGAGT 
GCTCTGAGAA CTGCTTTCAA AGGCCTTCAC CTTCGACCTG TGATGCCTTC TCAAATAGGA 
GACAGGAAGT GGCTTCATGG ATTACAGGCA GCCCTCCAGA ACTTTCTCAA GGGATATCCT 
GCAATTGTTC GGCAACTGTA CTCAGCAGGC GAAGGCAAAA GTGACACCAG TCAAAAGAAA 
TCTAAAGAAC TTCTGGAGTC CCTTCTCCAA GCTGACATTG TTAAATTTGC CCACTTCTTG 
TTGGATGTCA TTAATGTCCT TAGCATTCTG TCTGGTGTCA GTAAGAATAG AAATTCCTCA 
ATTGCTGACA TATTTGCCAC TTTAGAGTCA ATGCTGGAAA TGCTCCAAAG GTATCAAACA 
AGACTAGGGC CAAAAGAACG CATGATGGAT TCAGCCACAC ACTTTCATGG TAACTGCCTC 
AGAGGGAAAG AAAACATTTC TGCTGTGAGA AACATTGTTT TAACACATCT TATCAAGAGG 
CTTCAAGGCT GTTTCAGAGA TGCCAGCCAA AATGTGGTGA GGGCTACCAT GATTGGAAGT 
TTTAAATTAT GGCCCACAAA GATAAATCAA GAATTTGGAG AAAAAGAGGT ATCCATCCTG 
ACTGCACATT ATGAACCAGT ATTAGAAGCT GCCAATGTGA AACTTAGTGA AGTGGATGCT 
GAATGGAGCA TGTTGAAATT AGAGATTTAT GCCAGATTTC CGAACATTCA AAAATTGACC 
TGGGATTTTG TGAATTCCAT TTACTTTCAC AAGTATCCAA ATATCCTGAC ACTGGTCGAC 
CTAGTGTTGA CCCTTCCTGC AAGTTCAGCT GAAGCAGAAC GTGGCTTTAG TCAGATGAAA 
TGGACCAAGT CACACATGCA TGCAAAAATC AAGACTGAAA TTATGACGGA TGTCCTAATT 
ATTCAGTTGA ATTCTCCAGA CATTAATAAT TTTGACCCTA GGAAAGCTAT CCATTTATGG 
AATACAAGAA CACTGTCTTC AACTGGTGAC ACAAGACCTA ATTCAGATTG CTCATCAAAC 
TCAGAAAGCC ATTATGAAAG TGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007959539.1
CDS	107..6613
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGFLLGEVLYKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQPLTNLRLQHMKSETFRERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIKKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVANEIKKFEALIKKDLQAKESASKTTLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYKKHYSICAEILDQIVDLSTKVADYRTLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLIELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPMRILSIDTLVQEAIQAFHDNEKVSEILPIQKKDEEDALPVLQEEIKESQASDPQNVFSAGPVSNEGLPETEGANADKTPKAEEIKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINEIPVNQHWILDGFPMTLNQAQLLEEALTGYNRNLTELERKKAQKSTLAIDPATSKEVPLPSPAFDFVVLLDVSDTSSMSRMKDIIAEELSYKTAHEDISQRVAAENQDKGGDQNLRDQIQHRIIGFLDNWPLLKQWFSEPENILIKINAEIDKESLCEKVKEILTTEIVKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHLHHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINENWLQDSLGMTMNHFFSLMQAELNRFQDTKRLLQDYYRGMESKIPIEDNKRFTQIPLVQLDNKDNSESQLRIPLFPRISISLETVTPKPKTKSILKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKLKGKSPPIAEATPVIVTTEEIAEIERKNELRLKIKEEHLAALQFEETATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASVEKLTDIARYHIETSTKIQNELYLNQEDFFINGNVKVFPDPPPPIHPPPVEKEEDGTLTIEQLDNLRDQFLDMAPKGRNPSDDVNRVTEKQKRPQKLDHYLPKRSHQFLKFWLKKYSWLIYDELLNLMFCALCRKHGVKSGGSQVNFFYGTDNFRTQFLDAHQLSEAHAKASLMEATSSSPVNRATTKLMMRTMSKVTLGRVENLFRSCHAIAKTGRTLKDFIWMCKLDDMKGVDIGPVFRTKNSARMFTYFIAEVERKNLREKLEKSKFFSVISDGITDSLIKEVELVYVQFAHAGKVHCQFVGVQTVERNDPLAIKNATEKTLEINLQLRLSSQDWAKKLVGFGSDGIHGIEGENNEVALLLREIQPCVQTVYCFAHHLELSYKVLFQNIPLYNDVKDLLHSIYHFYHNSPLHKSALRTAFKGLHLRPVMPSQIGDRKWLHGLQAALQNFLKGYPAIVRQLYSAGEGKSDTSQKKSKELLESLLQADIVKFAHFLLDVINVLSILSGVSKNRNSSIADIFATLESMLEMLQRYQTRLGPKERMMDSATHFHGNCLRGKENISAVRNIVLTHLIKRLQGCFRDASQNVVRATMIGSFKLWPTKINQEFGEKEVSILTAHYEPVLEAANVKLSEVDAEWSMLKLEIYARFPNIQKLTWDFVNSIYFHKYPNILTLVDLVLTLPASSAEAERGFSQMKWTKSHMHAKIKTEIMTDVLIIQLNSPDINNFDPRKAIHLWNTRTLSSTGDTRPNSDCSSNSESHYESD

Target ORF information:

RefSeq Version	XM_007961348.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007961348.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG CAAGTGATCC ACAAAATGTA 
TTTTCAGCTG GTCCAGTTTC AAATGAAGGA TTACCAGAAA CAGAAGGTGC TAATGCTGAT 
AAAACACCAA AAGCTGAAGA AATCAAATCC AGTGACAGTT TCTTAAAACT CACTACGCGT 
GCTCAACTTG GTGCAAAATC AGAACAGTTG CTGAAGAAAG GAAAGAGCAT TCCTGACGTG 
CTGCTTGTTG ACATCATGGT AAATGCTATT AACGAGATAC CTGTGAATCA GCACTGGATC 
CTAGATGGTT TTCCAATGAC TTTAAATCAA GCACAGCTTC TGGAAGAAGC TCTTACAGGC 
TACAATAGAA ACCTCACAGA ACTGGAGAGA AAAAAAGCAC AAAAATCCAC ATTGGCTATT 
GATCCTGCGA CTTCCAAAGA AGTACCTCTT CCCTCTCCTG CATTTGATTT TGTCGTATTA 
TTAGACGTTT CAGATACTTC CTCAATGAGT CGCATGAAGG ATATAATAGC TGAAGAATTG 
TCCTATAAAA CTGCTCACGA AGACATCAGT CAACGTGTAG CTGCTGAAAA TCAAGATAAG 
GGTGGAGACC AAAATTTAAG AGACCAGATA CAACATAGAA TTATAGGCTT CTTGGACAAC 
TGGCCTTTAT TGAAGCAATG GTTTTCAGAG CCAGAAAATA TTTTGATAAA AATCAATGCT 
GAAATAGATA AGGAATCTTT ATGCGAAAAA GTAAAAGAAA TTCTTACGAC TGAAATAGTG 
AAAAAAAAGA ATAAAGTTGA GAAGAAATTA GAAGAAAAGG AAGCTGAGAA AAAAGCAGCA 
GCTTCCCTGG CTGAGCTTCC ACTTCCTACA CCTCCTCCTG CTCCTCCTCC TGAACCAGAA 
AAAGAGAAGG AAATTCATCT TCATCATGTG CCTTCAAAAA CTCCTACTGC AAAAGGAAAA 
CCTCAATCAG AAGCCCCGCA TGGTAAGCAA GAATCTCTTC AGGAAGGAAA AGGGAAGAAA 
GGTGAAACGG CACTCAAAAG AAAAGGTTCT CCTAAAGGAA AATCATCAGG AGGAAAAGTA 
CCAGTAAAGA AATCACCTGC TGACTCTACA GACACATCAC CTGTTGCAAT AGTGCCGCAG 
CCACCTAAGC CAGGATCAGA AGAATGGGTC TATGTGAATG AACCAGTTCC TGAGGAAATG 
CCTTCGTTTT TAGTACCTTA CTGGGAACTA ATAGAAAATT CCTATATAAA CACCATCAAA 
ACAGTACTCA GGCATCTGAG GGAAGACCAG CATACTGTGC TTGCTTACTT ATATGAGATT 
AGAACAAGTT TCCAGGAGTT TCTAAAGCGT CCAGATCACA AGCAAGATTT TGTAGCTCAA 
TGGCAGGCTG ATTTCAATTC CCTTCCTGAT GACCTGTGGG ATGATGAGGA AACAAAGGCT 
GAACTACACC AACGAGTGAA TGATCTGCGA GACCGCCTGT GGGACATTTG TGACGCACGG 
AAGGAAGAGG CAGAGCAGGA GCGGCTTGAC ATCATTAATG AGAACTGGTT ACAGGACTCT 
CTTGGAATGA CAATGAACCA TTTCTTTTCC CTGATGCAGG CAGAACTGAA CCGTTTCCAA 
GATACGAAGA GACTCCTTCA AGATTATTAC CGGGGAATGG AAAGTAAAAT CCCAATAGAG 
GACAATAAGA GATTTACTCA AATCCCTTTG GTCCAGCTGG ATAATAAAGA CAATTCTGAA 
AGCCAGCTTA GAATTCCTCT ATTTCCTCGA ATATCCATTT CTCTGGAAAC AGTTACGCCC 
AAACCAAAAA CAAAATCAAT ACTGAAGGGC AAGATGGATA ACTCCCTAGA AAACGTTGAG 
TCCAACTTTG AGGCTGATGA AAAGTTGGTC ATGGACACCT GGCAACAGGC TTCTTTAGCA 
GTATCTCACA TGGTGGCTGC AGAAATTCAT CAGAGGCTCA TGGAAGAAGA AAAAGAAAAC 
CAGCCAGCAG ACCCCAAAGA AAAATCTCCT CAGATGGGTG CAAATAAAAA AGTCAAAAAG 
GAGCCACCCA AGAAAAAACA GGAAGACAAA AAACTCAAAG GTAAATCACC ACCTATTGCA 
GAAGCAACTC CTGTCATAGT AACAACAGAG GAAATTGCTG AAATCGAAAG GAAAAATGAA 
CTGAGGCTCA AAATAAAAGA AGAACACCTT GCTGCCTTGC AATTTGAAGA AACAGCCACA 
CAGTTTCGAC TTGAACTGAT AAAGACAAAA GCATTGGCTC TTCTTGAAGA TTTAGTAACA 
AAGGTGGTTG ATGTATATAA ACTCATGGAA AAATGGCTTG GTGAGAGATA TTTGAATGAA 
ATGGCCAGTG TAGAAAAATT AACTGACATA GCTCGCTATC ACATTGAAAC ATCTACAAAA 
ATTCAGAATG AACTTTATTT AAACCAAGAA GACTTCTTCA TTAATGGCAA TGTAAAAGTC 
TTTCCAGATC CTCCCCCACC GATACATCCT CCACCTGTAG AAAAGGAAGA AGATGGCACC 
CTGACCATCG AACAGCTTGA CAATCTTCGG GATCAGTTCT TAGATATGGC ACCTAAAGGC 
AGGAATCCTT CAGATGATGT GAATAGAGTC ACCGAAAAGC AGAAAAGGCC ACAGAAGCTT 
GACCATTACC TTCCAAAAAG AAGCCATCAA TTTTTGAAAT TTTGGCTTAA AAAATATTCA 
TGGCTTATAT ATGATGAGCT ATTAAATCTT ATGTTCTGCG CCCTGTGTCG TAAGCATGGA 
GTGAAGTCAG GGGGAAGTCA AGTGAATTTC TTTTATGGTA CTGACAACTT TAGGACTCAA 
TTTCTCGATG CACATCAACT CAGCGAGGCT CATGCCAAGG CTTCATTAAT GGAAGCAACA 
AGTAGCTCTC CAGTGAACAG AGCCACCACC AAACTAATGA TGAGGACCAT GAGCAAAGTA 
ACCCTCGGGA GAGTAGAGAA CTTATTCAGA TCATGCCATG CTATTGCCAA GACCGGACGT 
ACCCTCAAAG ATTTTATTTG GATGTGCAAA TTGGATGATA TGAAAGGTGT TGATATTGGC 
CCAGTATTCA GAACTAAAAA CTCAGCAAGA ATGTTTACAT ACTTTATTGC TGAAGTAGAA 
CGAAAAAATC TAAGAGAGAA ACTAGAAAAA AGTAAATTTT TTTCTGTCAT CAGTGATGGA 
ATCACAGACA GTTTAATCAA AGAAGTCGAA CTTGTTTATG TGCAGTTTGC ACATGCAGGA 
AAAGTGCACT GTCAATTTGT TGGGGTACAG ACAGTAGAAA GAAATGATCC TCTGGCAATA 
AAAAATGCCA CTGAGAAAAC TCTAGAGATA AATCTTCAAC TTAGACTGTC GAGCCAAGAC 
TGGGCAAAGA AGCTGGTTGG TTTTGGAAGT GATGGTATCC ATGGCATAGA GGGAGAGAAC 
AATGAGGTGG CCCTGCTACT ACGAGAAATA CAACCATGTG TACAGACTGT ATATTGCTTT 
GCACATCACC TTGAACTATC TTACAAAGTG CTGTTCCAGA ACATTCCTCT GTACAATGAT 
GTCAAAGATC TCCTTCACAG CATTTATCAC TTTTATCACA ATTCTCCTCT TCATAAGAGT 
GCTCTGAGAA CTGCTTTCAA AGGCCTTCAC CTTCGACCTG TGATGCCTTC TCAAATAGGA 
GACAGGAAGT GGCTTCATGG ATTACAGGCA GCCCTCCAGA ACTTTCTCAA GGGATATCCT 
GCAATTGTTC GGCAACTGTA CTCAGCAGGC GAAGGCAAAA GTGACACCAG TCAAAAGAAA 
TCTAAAGAAC TTCTGGAGTC CCTTCTCCAA GCTGACATTG TTAAATTTGC CCACTTCTTG 
TTGGATGTCA TTAATGTCCT TAGCATTCTG TCTGGTGTCA GTAAGAATAG AAATTCCTCA 
ATTGCTGACA TATTTGCCAC TTTAGAGTCA ATGCTGGAAA TGCTCCAAAG GTATCAAACA 
AGACTAGGGC CAAAAGAACG CATGATGGAT TCAGCCACAC ACTTTCATGG TAACTGCCTC 
AGAGGGAAAG AAAACATTTC TGCTGTGAGA AACATTGTTT TAACACATCT TATCAAGAGG 
CTTCAAGGCT GTTTCAGAGA TGCCAGCCAA AATGTGGTGA GGGCTACCAT GATTGGAAGT 
TTTAAATTAT GGCCCACAAA GATAAATCAA GAATTTGGAG AAAAAGAGGT ATCCATCCTG 
ACTGCACATT ATGAACCAGT ATTAGAAGCT GCCAATGTGA AACTTAGTGA AGTGGATGCT 
GAATGGAGCA TGTTGAAATT AGAGATTTAT GCCAGATTTC CGAACATTCA AAAATTGACC 
TGGGATTTTG TGAATTCCAT TTACTTTCAC AAGTATCCAA ATATCCTGAC ACTGGTCGAC 
CTAGTGTTGA CCCTTCCTGC AAGTTCAGCT GAAGCAGAAC GTGGCTTTAG TCAGATGAAA 
TGGACCAAGT CACACATGCA TGCAAAAATC AAGACTGAAA TTATGACGGA TGTCCTAATT 
ATTCAGTTGA ATTCTCCAGA CATTAATAAT TTTGACCCTA GGAAAGCTAT CCATTTATGG 
AATACAAGAA CACTGTCTTC AACTGGTGAC ACAAGACCTA ATTCAGATTG CTCATCAAAC 
TCAGAAAGCC ATTATGAAAG TGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_007961349.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007959540.1 Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.

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CloneID	OCh83460
Clone ID Related Accession (Same CDS sequence)	XM_007961349.1
Accession Version	XM_007961349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	sperm flagellar protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735239.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAAAA TGTATTTTCA 
GCTGGTCCAG TTTCAAATGA AGGATTACCA GAAACAGAAG GTGCTAATGC TGATAAAACA 
CCAAAAGCTG AAGAAATCAA ATCCAGTGAC AGTTTCTTAA AACTCACTAC GCGTGCTCAA 
CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA CGTGCTGCTT 
GTTGACATCA TGGTAAATGC TATTAACGAG ATACCTGTGA ATCAGCACTG GATCCTAGAT 
GGTTTTCCAA TGACTTTAAA TCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTACAAT 
AGAAACCTCA CAGAACTGGA GAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT 
GCGACTTCCA AAGAAGTACC TCTTCCCTCT CCTGCATTTG ATTTTGTCGT ATTATTAGAC 
GTTTCAGATA CTTCCTCAAT GAGTCGCATG AAGGATATAA TAGCTGAAGA ATTGTCCTAT 
AAAACTGCTC ACGAAGACAT CAGTCAACGT GTAGCTGCTG AAAATCAAGA TAAGGGTGGA 
GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT 
TTATTGAAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA 
GATAAGGAAT CTTTATGCGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGTGAAAAAA 
AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC 
CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG 
AAGGAAATTC ATCTTCATCA TGTGCCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA 
TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAA 
ACGGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA 
AAGAAATCAC CTGCTGACTC TACAGACACA TCACCTGTTG CAATAGTGCC GCAGCCACCT 
AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTCG 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA 
CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA 
AGTTTCCAGG AGTTTCTAAA GCGTCCAGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ATTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA 
CACCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGACGC ACGGAAGGAA 
GAGGCAGAGC AGGAGCGGCT TGACATCATT AATGAGAACT GGTTACAGGA CTCTCTTGGA 
ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAAC TGAACCGTTT CCAAGATACG 
AAGAGACTCC TTCAAGATTA TTACCGGGGA ATGGAAAGTA AAATCCCAAT AGAGGACAAT 
AAGAGATTTA CTCAAATCCC TTTGGTCCAG CTGGATAATA AAGACAATTC TGAAAGCCAG 
CTTAGAATTC CTCTATTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC GCCCAAACCA 
AAAACAAAAT CAATACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC 
TTTGAGGCTG ATGAAAAGTT GGTCATGGAC ACCTGGCAAC AGGCTTCTTT AGCAGTATCT 
CACATGGTGG CTGCAGAAAT TCATCAGAGG CTCATGGAAG AAGAAAAAGA AAACCAGCCA 
GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA 
CCCAAGAAAA AACAGGAAGA CAAAAAACTC AAAGGTAAAT CACCACCTAT TGCAGAAGCA 
ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCG AAAGGAAAAA TGAACTGAGG 
CTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAACAGC CACACAGTTT 
CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG 
GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GATATTTGAA TGAAATGGCC 
AGTGTAGAAA AATTAACTGA CATAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG 
AATGAACTTT ATTTAAACCA AGAAGACTTC TTCATTAATG GCAATGTAAA AGTCTTTCCA 
GATCCTCCCC CACCGATACA TCCTCCACCT GTAGAAAAGG AAGAAGATGG CACCCTGACC 
ATCGAACAGC TTGACAATCT TCGGGATCAG TTCTTAGATA TGGCACCTAA AGGCAGGAAT 
CCTTCAGATG ATGTGAATAG AGTCACCGAA AAGCAGAAAA GGCCACAGAA GCTTGACCAT 
TACCTTCCAA AAAGAAGCCA TCAATTTTTG AAATTTTGGC TTAAAAAATA TTCATGGCTT 
ATATATGATG AGCTATTAAA TCTTATGTTC TGCGCCCTGT GTCGTAAGCA TGGAGTGAAG 
TCAGGGGGAA GTCAAGTGAA TTTCTTTTAT GGTACTGACA ACTTTAGGAC TCAATTTCTC 
GATGCACATC AACTCAGCGA GGCTCATGCC AAGGCTTCAT TAATGGAAGC AACAAGTAGC 
TCTCCAGTGA ACAGAGCCAC CACCAAACTA ATGATGAGGA CCATGAGCAA AGTAACCCTC 
GGGAGAGTAG AGAACTTATT CAGATCATGC CATGCTATTG CCAAGACCGG ACGTACCCTC 
AAAGATTTTA TTTGGATGTG CAAATTGGAT GATATGAAAG GTGTTGATAT TGGCCCAGTA 
TTCAGAACTA AAAACTCAGC AAGAATGTTT ACATACTTTA TTGCTGAAGT AGAACGAAAA 
AATCTAAGAG AGAAACTAGA AAAAAGTAAA TTTTTTTCTG TCATCAGTGA TGGAATCACA 
GACAGTTTAA TCAAAGAAGT CGAACTTGTT TATGTGCAGT TTGCACATGC AGGAAAAGTG 
CACTGTCAAT TTGTTGGGGT ACAGACAGTA GAAAGAAATG ATCCTCTGGC AATAAAAAAT 
GCCACTGAGA AAACTCTAGA GATAAATCTT CAACTTAGAC TGTCGAGCCA AGACTGGGCA 
AAGAAGCTGG TTGGTTTTGG AAGTGATGGT ATCCATGGCA TAGAGGGAGA GAACAATGAG 
GTGGCCCTGC TACTACGAGA AATACAACCA TGTGTACAGA CTGTATATTG CTTTGCACAT 
CACCTTGAAC TATCTTACAA AGTGCTGTTC CAGAACATTC CTCTGTACAA TGATGTCAAA 
GATCTCCTTC ACAGCATTTA TCACTTTTAT CACAATTCTC CTCTTCATAA GAGTGCTCTG 
AGAACTGCTT TCAAAGGCCT TCACCTTCGA CCTGTGATGC CTTCTCAAAT AGGAGACAGG 
AAGTGGCTTC ATGGATTACA GGCAGCCCTC CAGAACTTTC TCAAGGGATA TCCTGCAATT 
GTTCGGCAAC TGTACTCAGC AGGCGAAGGC AAAAGTGACA CCAGTCAAAA GAAATCTAAA 
GAACTTCTGG AGTCCCTTCT CCAAGCTGAC ATTGTTAAAT TTGCCCACTT CTTGTTGGAT 
GTCATTAATG TCCTTAGCAT TCTGTCTGGT GTCAGTAAGA ATAGAAATTC CTCAATTGCT 
GACATATTTG CCACTTTAGA GTCAATGCTG GAAATGCTCC AAAGGTATCA AACAAGACTA 
GGGCCAAAAG AACGCATGAT GGATTCAGCC ACACACTTTC ATGGTAACTG CCTCAGAGGG 
AAAGAAAACA TTTCTGCTGT GAGAAACATT GTTTTAACAC ATCTTATCAA GAGGCTTCAA 
GGCTGTTTCA GAGATGCCAG CCAAAATGTG GTGAGGGCTA CCATGATTGG AAGTTTTAAA 
TTATGGCCCA CAAAGATAAA TCAAGAATTT GGAGAAAAAG AGGTATCCAT CCTGACTGCA 
CATTATGAAC CAGTATTAGA AGCTGCCAAT GTGAAACTTA GTGAAGTGGA TGCTGAATGG 
AGCATGTTGA AATTAGAGAT TTATGCCAGA TTTCCGAACA TTCAAAAATT GACCTGGGAT 
TTTGTGAATT CCATTTACTT TCACAAGTAT CCAAATATCC TGACACTGGT CGACCTAGTG 
TTGACCCTTC CTGCAAGTTC AGCTGAAGCA GAACGTGGCT TTAGTCAGAT GAAATGGACC 
AAGTCACACA TGCATGCAAA AATCAAGACT GAAATTATGA CGGATGTCCT AATTATTCAG 
TTGAATTCTC CAGACATTAA TAATTTTGAC CCTAGGAAAG CTATCCATTT ATGGAATACA 
AGAACACTGT CTTCAACTGG TGACACAAGA CCTAATTCAG ATTGCTCATC AAACTCAGAA 
AGCCATTATG AAAGTGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007959540.1
CDS	107..6607
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGFLLGEVLYKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQPLTNLRLQHMKSETFRERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIKKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVANEIKKFEALIKKDLQAKESASKTTLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYKKHYSICAEILDQIVDLSTKVADYRTLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLIELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPMRILSIDTLVQEAIQAFHDNEKVSEILPIQKKDEEDALPVLQEEIKESQDPQNVFSAGPVSNEGLPETEGANADKTPKAEEIKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINEIPVNQHWILDGFPMTLNQAQLLEEALTGYNRNLTELERKKAQKSTLAIDPATSKEVPLPSPAFDFVVLLDVSDTSSMSRMKDIIAEELSYKTAHEDISQRVAAENQDKGGDQNLRDQIQHRIIGFLDNWPLLKQWFSEPENILIKINAEIDKESLCEKVKEILTTEIVKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHLHHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINENWLQDSLGMTMNHFFSLMQAELNRFQDTKRLLQDYYRGMESKIPIEDNKRFTQIPLVQLDNKDNSESQLRIPLFPRISISLETVTPKPKTKSILKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKLKGKSPPIAEATPVIVTTEEIAEIERKNELRLKIKEEHLAALQFEETATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASVEKLTDIARYHIETSTKIQNELYLNQEDFFINGNVKVFPDPPPPIHPPPVEKEEDGTLTIEQLDNLRDQFLDMAPKGRNPSDDVNRVTEKQKRPQKLDHYLPKRSHQFLKFWLKKYSWLIYDELLNLMFCALCRKHGVKSGGSQVNFFYGTDNFRTQFLDAHQLSEAHAKASLMEATSSSPVNRATTKLMMRTMSKVTLGRVENLFRSCHAIAKTGRTLKDFIWMCKLDDMKGVDIGPVFRTKNSARMFTYFIAEVERKNLREKLEKSKFFSVISDGITDSLIKEVELVYVQFAHAGKVHCQFVGVQTVERNDPLAIKNATEKTLEINLQLRLSSQDWAKKLVGFGSDGIHGIEGENNEVALLLREIQPCVQTVYCFAHHLELSYKVLFQNIPLYNDVKDLLHSIYHFYHNSPLHKSALRTAFKGLHLRPVMPSQIGDRKWLHGLQAALQNFLKGYPAIVRQLYSAGEGKSDTSQKKSKELLESLLQADIVKFAHFLLDVINVLSILSGVSKNRNSSIADIFATLESMLEMLQRYQTRLGPKERMMDSATHFHGNCLRGKENISAVRNIVLTHLIKRLQGCFRDASQNVVRATMIGSFKLWPTKINQEFGEKEVSILTAHYEPVLEAANVKLSEVDAEWSMLKLEIYARFPNIQKLTWDFVNSIYFHKYPNILTLVDLVLTLPASSAEAERGFSQMKWTKSHMHAKIKTEIMTDVLIIQLNSPDINNFDPRKAIHLWNTRTLSSTGDTRPNSDCSSNSESHYESD

Target ORF information:

RefSeq Version	XM_007961349.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007961349.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAAAA TGTATTTTCA 
GCTGGTCCAG TTTCAAATGA AGGATTACCA GAAACAGAAG GTGCTAATGC TGATAAAACA 
CCAAAAGCTG AAGAAATCAA ATCCAGTGAC AGTTTCTTAA AACTCACTAC GCGTGCTCAA 
CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA CGTGCTGCTT 
GTTGACATCA TGGTAAATGC TATTAACGAG ATACCTGTGA ATCAGCACTG GATCCTAGAT 
GGTTTTCCAA TGACTTTAAA TCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTACAAT 
AGAAACCTCA CAGAACTGGA GAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT 
GCGACTTCCA AAGAAGTACC TCTTCCCTCT CCTGCATTTG ATTTTGTCGT ATTATTAGAC 
GTTTCAGATA CTTCCTCAAT GAGTCGCATG AAGGATATAA TAGCTGAAGA ATTGTCCTAT 
AAAACTGCTC ACGAAGACAT CAGTCAACGT GTAGCTGCTG AAAATCAAGA TAAGGGTGGA 
GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT 
TTATTGAAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA 
GATAAGGAAT CTTTATGCGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGTGAAAAAA 
AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC 
CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG 
AAGGAAATTC ATCTTCATCA TGTGCCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA 
TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAA 
ACGGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA 
AAGAAATCAC CTGCTGACTC TACAGACACA TCACCTGTTG CAATAGTGCC GCAGCCACCT 
AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTCG 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA 
CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA 
AGTTTCCAGG AGTTTCTAAA GCGTCCAGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ATTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA 
CACCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGACGC ACGGAAGGAA 
GAGGCAGAGC AGGAGCGGCT TGACATCATT AATGAGAACT GGTTACAGGA CTCTCTTGGA 
ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAAC TGAACCGTTT CCAAGATACG 
AAGAGACTCC TTCAAGATTA TTACCGGGGA ATGGAAAGTA AAATCCCAAT AGAGGACAAT 
AAGAGATTTA CTCAAATCCC TTTGGTCCAG CTGGATAATA AAGACAATTC TGAAAGCCAG 
CTTAGAATTC CTCTATTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC GCCCAAACCA 
AAAACAAAAT CAATACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC 
TTTGAGGCTG ATGAAAAGTT GGTCATGGAC ACCTGGCAAC AGGCTTCTTT AGCAGTATCT 
CACATGGTGG CTGCAGAAAT TCATCAGAGG CTCATGGAAG AAGAAAAAGA AAACCAGCCA 
GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA 
CCCAAGAAAA AACAGGAAGA CAAAAAACTC AAAGGTAAAT CACCACCTAT TGCAGAAGCA 
ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCG AAAGGAAAAA TGAACTGAGG 
CTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAACAGC CACACAGTTT 
CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG 
GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GATATTTGAA TGAAATGGCC 
AGTGTAGAAA AATTAACTGA CATAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG 
AATGAACTTT ATTTAAACCA AGAAGACTTC TTCATTAATG GCAATGTAAA AGTCTTTCCA 
GATCCTCCCC CACCGATACA TCCTCCACCT GTAGAAAAGG AAGAAGATGG CACCCTGACC 
ATCGAACAGC TTGACAATCT TCGGGATCAG TTCTTAGATA TGGCACCTAA AGGCAGGAAT 
CCTTCAGATG ATGTGAATAG AGTCACCGAA AAGCAGAAAA GGCCACAGAA GCTTGACCAT 
TACCTTCCAA AAAGAAGCCA TCAATTTTTG AAATTTTGGC TTAAAAAATA TTCATGGCTT 
ATATATGATG AGCTATTAAA TCTTATGTTC TGCGCCCTGT GTCGTAAGCA TGGAGTGAAG 
TCAGGGGGAA GTCAAGTGAA TTTCTTTTAT GGTACTGACA ACTTTAGGAC TCAATTTCTC 
GATGCACATC AACTCAGCGA GGCTCATGCC AAGGCTTCAT TAATGGAAGC AACAAGTAGC 
TCTCCAGTGA ACAGAGCCAC CACCAAACTA ATGATGAGGA CCATGAGCAA AGTAACCCTC 
GGGAGAGTAG AGAACTTATT CAGATCATGC CATGCTATTG CCAAGACCGG ACGTACCCTC 
AAAGATTTTA TTTGGATGTG CAAATTGGAT GATATGAAAG GTGTTGATAT TGGCCCAGTA 
TTCAGAACTA AAAACTCAGC AAGAATGTTT ACATACTTTA TTGCTGAAGT AGAACGAAAA 
AATCTAAGAG AGAAACTAGA AAAAAGTAAA TTTTTTTCTG TCATCAGTGA TGGAATCACA 
GACAGTTTAA TCAAAGAAGT CGAACTTGTT TATGTGCAGT TTGCACATGC AGGAAAAGTG 
CACTGTCAAT TTGTTGGGGT ACAGACAGTA GAAAGAAATG ATCCTCTGGC AATAAAAAAT 
GCCACTGAGA AAACTCTAGA GATAAATCTT CAACTTAGAC TGTCGAGCCA AGACTGGGCA 
AAGAAGCTGG TTGGTTTTGG AAGTGATGGT ATCCATGGCA TAGAGGGAGA GAACAATGAG 
GTGGCCCTGC TACTACGAGA AATACAACCA TGTGTACAGA CTGTATATTG CTTTGCACAT 
CACCTTGAAC TATCTTACAA AGTGCTGTTC CAGAACATTC CTCTGTACAA TGATGTCAAA 
GATCTCCTTC ACAGCATTTA TCACTTTTAT CACAATTCTC CTCTTCATAA GAGTGCTCTG 
AGAACTGCTT TCAAAGGCCT TCACCTTCGA CCTGTGATGC CTTCTCAAAT AGGAGACAGG 
AAGTGGCTTC ATGGATTACA GGCAGCCCTC CAGAACTTTC TCAAGGGATA TCCTGCAATT 
GTTCGGCAAC TGTACTCAGC AGGCGAAGGC AAAAGTGACA CCAGTCAAAA GAAATCTAAA 
GAACTTCTGG AGTCCCTTCT CCAAGCTGAC ATTGTTAAAT TTGCCCACTT CTTGTTGGAT 
GTCATTAATG TCCTTAGCAT TCTGTCTGGT GTCAGTAAGA ATAGAAATTC CTCAATTGCT 
GACATATTTG CCACTTTAGA GTCAATGCTG GAAATGCTCC AAAGGTATCA AACAAGACTA 
GGGCCAAAAG AACGCATGAT GGATTCAGCC ACACACTTTC ATGGTAACTG CCTCAGAGGG 
AAAGAAAACA TTTCTGCTGT GAGAAACATT GTTTTAACAC ATCTTATCAA GAGGCTTCAA 
GGCTGTTTCA GAGATGCCAG CCAAAATGTG GTGAGGGCTA CCATGATTGG AAGTTTTAAA 
TTATGGCCCA CAAAGATAAA TCAAGAATTT GGAGAAAAAG AGGTATCCAT CCTGACTGCA 
CATTATGAAC CAGTATTAGA AGCTGCCAAT GTGAAACTTA GTGAAGTGGA TGCTGAATGG 
AGCATGTTGA AATTAGAGAT TTATGCCAGA TTTCCGAACA TTCAAAAATT GACCTGGGAT 
TTTGTGAATT CCATTTACTT TCACAAGTAT CCAAATATCC TGACACTGGT CGACCTAGTG 
TTGACCCTTC CTGCAAGTTC AGCTGAAGCA GAACGTGGCT TTAGTCAGAT GAAATGGACC 
AAGTCACACA TGCATGCAAA AATCAAGACT GAAATTATGA CGGATGTCCT AATTATTCAG 
TTGAATTCTC CAGACATTAA TAATTTTGAC CCTAGGAAAG CTATCCATTT ATGGAATACA 
AGAACACTGT CTTCAACTGG TGACACAAGA CCTAATTCAG ATTGCTCATC AAACTCAGAA 
AGCCATTATG AAAGTGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_007961350.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007959541.1 Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.

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CloneID	OCh83461
Clone ID Related Accession (Same CDS sequence)	XM_007961350.1
Accession Version	XM_007961350.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	sperm flagellar protein 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735239.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG CAAGTGATCC ACAAAATGTA 
TTTTCAGCTG GTCCAGTTTC AAATGAAGGA TTACCAGAAA CAGAAGGTGA AACGATACTA 
AGTGCTAATG CTGATAAAAC ACCAAAAGCT GAAGAAATCA AATCCAGTGA CAGTTTCTTA 
AAACTCACTA CGCGTGCTCA ACTTGGTGCA AAATCAGAAC AGTTGCTGAA GAAAGGAAAG 
AGCATTCCTG ACGTGCTGCT TGTTGACATC ATGGTAAATG CTATTAACGA GATACCTGTG 
AATCAGCACT GGATCCTAGA TGGTTTTCCA ATGACTTTAA ATCAAGCACA GCTTCTGGAA 
GAAGCTCTTA CAGGCTACAA TAGAAACCTC ACAGAACTGG AGAGAAAAAA AGCACAAAAA 
TCCACATTGG CTATTGATCC TGCGACTTCC AAAGAAGTAC CTCTTCCCTC TCCTGCATTT 
GATTTTGTCG TATTATTAGA CGTTTCAGAT ACTTCCTCAA TGAGTCGCAT GAAGGATATA 
ATAGCTGAAG AATTGTCCTA TAAAACTGCT CACGAAGACA TCAGTCAACG TGTAGCTGCT 
GAAAATCAAG ATAAGGGTGG AGACCAAAAT TTAAGAGACC AGATACAACA TAGAATTATA 
GGCTTCTTGG ACAACTGGCC TTTATTGAAG CAATGGTTTT CAGAGCCAGA AAATATTTTG 
ATAAAAATCA ATGCTGAAAT AGATAAGGAA TCTTTATGCG AAAAAGTAAA AGAAATTCTT 
ACGACTGAAA TAGTGAAAAA AAAGAATAAA GTTGAGAAGA AATTAGAAGA AAAGGAAGCT 
GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG CTTCCACTTC CTACACCTCC TCCTGCTCCT 
CCTCCTGAAC CAGAAAAAGA GAAGGAAATT CATCTTCATC ATGTGCCTTC AAAAACTCCT 
ACTGCAAAAG GAAAACCTCA ATCAGGTTCT CCTAAAGGAA AATCATCAGG AGGAAAAGTA 
CCAGTAAAGA AATCACCTGC TGACTCTACA GACACATCAC CTGTTGCAAT AGTGCCGCAG 
CCACCTAAGC CAGGATCAGA AGAATGGGTC TATGTGAATG AACCAGTTCC TGAGGAAATG 
CCTTCGTTTT TAGTACCTTA CTGGGAACTA ATAGAAAATT CCTATATAAA CACCATCAAA 
ACAGTACTCA GGCATCTGAG GGAAGACCAG CATACTGTGC TTGCTTACTT ATATGAGATT 
AGAACAAGTT TCCAGGAGTT TCTAAAGCGT CCAGATCACA AGCAAGATTT TGTAGCTCAA 
TGGCAGGCTG ATTTCAATTC CCTTCCTGAT GACCTGTGGG ATGATGAGGA AACAAAGGCT 
GAACTACACC AACGAGTGAA TGATCTGCGA GACCGCCTGT GGGACATTTG TGACGCACGG 
AAGGAAGAGG CAGAGCAGGA GCGGCTTGAC ATCATTAATG AGAACTGGTT ACAGGACTCT 
CTTGGAATGA CAATGAACCA TTTCTTTTCC CTGATGCAGG CAGAACTGAA CCGTTTCCAA 
GATACGAAGA GACTCCTTCA AGATTATTAC CGGGGAATGG AAAGTAAAAT CCCAATAGAG 
GACAATAAGA GATTTACTCA AATCCCTTTG GTCCAGCTGG ATAATAAAGA CAATTCTGAA 
AGCCAGCTTA GAATTCCTCT ATTTCCTCGA ATATCCATTT CTCTGGAAAC AGTTACGCCC 
AAACCAAAAA CAAAATCAAT ACTGAAGGGC AAGATGGATA ACTCCCTAGA AAACGTTGAG 
TCCAACTTTG AGGCTGATGA AAAGTTGGTC ATGGACACCT GGCAACAGGC TTCTTTAGCA 
GTATCTCACA TGGTGGCTGC AGAAATTCAT CAGAGGCTCA TGGAAGAAGA AAAAGAAAAC 
CAGCCAGCAG ACCCCAAAGA AAAATCTCCT CAGATGGGTG CAAATAAAAA AGTCAAAAAG 
GAGCCACCCA AGAAAAAACA GGAAGACAAA AAACTCAAAG GTAAATCACC ACCTATTGCA 
GAAGCAACTC CTGTCATAGT AACAACAGAG GAAATTGCTG AAATCGAAAG GAAAAATGAA 
CTGAGGCTCA AAATAAAAGA AGAACACCTT GCTGCCTTGC AATTTGAAGA AACAGCCACA 
CAGTTTCGAC TTGAACTGAT AAAGACAAAA GCATTGGCTC TTCTTGAAGA TTTAGTAACA 
AAGGTGGTTG ATGTATATAA ACTCATGGAA AAATGGCTTG GTGAGAGATA TTTGAATGAA 
ATGGCCAGTG TAGAAAAATT AACTGACATA GCTCGCTATC ACATTGAAAC ATCTACAAAA 
ATTCAGAATG AACTTTATTT AAACCAAGAA GACTTCTTCA TTAATGGCAA TGTAAAAGTC 
TTTCCAGATC CTCCCCCACC GATACATCCT CCACCTGTAG AAAAGGAAGA AGATGGCACC 
CTGACCATCG AACAGCTTGA CAATCTTCGG GATCAGTTCT TAGATATGGC ACCTAAAGGC 
AGGAATCCTT CAGATGATGT GAATAGAGTC ACCGAAAAGC AGAAAAGGCC ACAGAAGCTT 
GACCATTACC TTCCAAAAAG AAGCCATCAA TTTTTGAAAT TTTGGCTTAA AAAATATTCA 
TGGCTTATAT ATGATGAGCT ATTAAATCTT ATGTTCTGCG CCCTGTGTCG TAAGCATGGA 
GTGAAGTCAG GGGGAAGTCA AGTGAATTTC TTTTATGGTA CTGACAACTT TAGGACTCAA 
TTTCTCGATG CACATCAACT CAGCGAGGCT CATGCCAAGG CTTCATTAAT GGAAGCAACA 
AGTAGCTCTC CAGTGAACAG AGCCACCACC AAACTAATGA TGAGGACCAT GAGCAAAGTA 
ACCCTCGGGA GAGTAGAGAA CTTATTCAGA TCATGCCATG CTATTGCCAA GACCGGACGT 
ACCCTCAAAG ATTTTATTTG GATGTGCAAA TTGGATGATA TGAAAGGTGT TGATATTGGC 
CCAGTATTCA GAACTAAAAA CTCAGCAAGA ATGTTTACAT ACTTTATTGC TGAAGTAGAA 
CGAAAAAATC TAAGAGAGAA ACTAGAAAAA AGTAAATTTT TTTCTGTCAT CAGTGATGGA 
ATCACAGACA GTTTAATCAA AGAAGTCGAA CTTGTTTATG TGCAGTTTGC ACATGCAGGA 
AAAGTGCACT GTCAATTTGT TGGGGTACAG ACAGTAGAAA GAAATGATCC TCTGGCAATA 
AAAAATGCCA CTGAGAAAAC TCTAGAGATA AATCTTCAAC TTAGACTGTC GAGCCAAGAC 
TGGGCAAAGA AGCTGGTTGG TTTTGGAAGT GATGGTATCC ATGGCATAGA GGGAGAGAAC 
AATGAGGTGG CCCTGCTACT ACGAGAAATA CAACCATGTG TACAGACTGT ATATTGCTTT 
GCACATCACC TTGAACTATC TTACAAAGTG CTGTTCCAGA ACATTCCTCT GTACAATGAT 
GTCAAAGATC TCCTTCACAG CATTTATCAC TTTTATCACA ATTCTCCTCT TCATAAGAGT 
GCTCTGAGAA CTGCTTTCAA AGGCCTTCAC CTTCGACCTG TGATGCCTTC TCAAATAGGA 
GACAGGAAGT GGCTTCATGG ATTACAGGCA GCCCTCCAGA ACTTTCTCAA GGGATATCCT 
GCAATTGTTC GGCAACTGTA CTCAGCAGGC GAAGGCAAAA GTGACACCAG TCAAAAGAAA 
TCTAAAGAAC TTCTGGAGTC CCTTCTCCAA GCTGACATTG TTAAATTTGC CCACTTCTTG 
TTGGATGTCA TTAATGTCCT TAGCATTCTG TCTGGTGTCA GTAAGAATAG AAATTCCTCA 
ATTGCTGACA TATTTGCCAC TTTAGAGTCA ATGCTGGAAA TGCTCCAAAG GTATCAAACA 
AGACTAGGGC CAAAAGAACG CATGATGGAT TCAGCCACAC ACTTTCATGG TAACTGCCTC 
AGAGGGAAAG AAAACATTTC TGCTGTGAGA AACATTGTTT TAACACATCT TATCAAGAGG 
CTTCAAGGCT GTTTCAGAGA TGCCAGCCAA AATGTGGTGA GGGCTACCAT GATTGGAAGT 
TTTAAATTAT GGCCCACAAA GATAAATCAA GAATTTGGAG AAAAAGAGGT ATCCATCCTG 
ACTGCACATT ATGAACCAGT ATTAGAAGCT GCCAATGTGA AACTTAGTGA AGTGGATGCT 
GAATGGAGCA TGTTGAAATT AGAGATTTAT GCCAGATTTC CGAACATTCA AAAATTGACC 
TGGGATTTTG TGAATTCCAT TTACTTTCAC AAGTATCCAA ATATCCTGAC ACTGGTCGAC 
CTAGTGTTGA CCCTTCCTGC AAGTTCAGCT GAAGCAGAAC GTGGCTTTAG TCAGATGAAA 
TGGACCAAGT CACACATGCA TGCAAAAATC AAGACTGAAA TTATGACGGA TGTCCTAATT 
ATTCAGTTGA ATTCTCCAGA CATTAATAAT TTTGACCCTA GGAAAGCTAT CCATTTATGG 
AATACAAGAA CACTGTCTTC AACTGGTGAC ACAAGACCTA ATTCAGATTG CTCATCAAAC 
TCAGAAAGCC ATTATGAAAG TGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007959541.1
CDS	107..6553
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGFLLGEVLYKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQPLTNLRLQHMKSETFRERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIKKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVANEIKKFEALIKKDLQAKESASKTTLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYKKHYSICAEILDQIVDLSTKVADYRTLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLIELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPMRILSIDTLVQEAIQAFHDNEKVSEILPIQKKDEEDALPVLQEEIKESQASDPQNVFSAGPVSNEGLPETEGETILSANADKTPKAEEIKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINEIPVNQHWILDGFPMTLNQAQLLEEALTGYNRNLTELERKKAQKSTLAIDPATSKEVPLPSPAFDFVVLLDVSDTSSMSRMKDIIAEELSYKTAHEDISQRVAAENQDKGGDQNLRDQIQHRIIGFLDNWPLLKQWFSEPENILIKINAEIDKESLCEKVKEILTTEIVKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHLHHVPSKTPTAKGKPQSGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINENWLQDSLGMTMNHFFSLMQAELNRFQDTKRLLQDYYRGMESKIPIEDNKRFTQIPLVQLDNKDNSESQLRIPLFPRISISLETVTPKPKTKSILKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKLKGKSPPIAEATPVIVTTEEIAEIERKNELRLKIKEEHLAALQFEETATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASVEKLTDIARYHIETSTKIQNELYLNQEDFFINGNVKVFPDPPPPIHPPPVEKEEDGTLTIEQLDNLRDQFLDMAPKGRNPSDDVNRVTEKQKRPQKLDHYLPKRSHQFLKFWLKKYSWLIYDELLNLMFCALCRKHGVKSGGSQVNFFYGTDNFRTQFLDAHQLSEAHAKASLMEATSSSPVNRATTKLMMRTMSKVTLGRVENLFRSCHAIAKTGRTLKDFIWMCKLDDMKGVDIGPVFRTKNSARMFTYFIAEVERKNLREKLEKSKFFSVISDGITDSLIKEVELVYVQFAHAGKVHCQFVGVQTVERNDPLAIKNATEKTLEINLQLRLSSQDWAKKLVGFGSDGIHGIEGENNEVALLLREIQPCVQTVYCFAHHLELSYKVLFQNIPLYNDVKDLLHSIYHFYHNSPLHKSALRTAFKGLHLRPVMPSQIGDRKWLHGLQAALQNFLKGYPAIVRQLYSAGEGKSDTSQKKSKELLESLLQADIVKFAHFLLDVINVLSILSGVSKNRNSSIADIFATLESMLEMLQRYQTRLGPKERMMDSATHFHGNCLRGKENISAVRNIVLTHLIKRLQGCFRDASQNVVRATMIGSFKLWPTKINQEFGEKEVSILTAHYEPVLEAANVKLSEVDAEWSMLKLEIYARFPNIQKLTWDFVNSIYFHKYPNILTLVDLVLTLPASSAEAERGFSQMKWTKSHMHAKIKTEIMTDVLIIQLNSPDINNFDPRKAIHLWNTRTLSSTGDTRPNSDCSSNSESHYESD

Target ORF information:

RefSeq Version	XM_007961350.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007961350.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGAGACTT 
AGACACATGA TCCCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATACA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCA AAAAGCAATA CTTAAACAGA 
AGACGACAAA ACGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAGGATG TGGCCAATGA AATTAAGAAG TTCGAAGCAT TAATAAAGAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTACTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCGGATGA TGAATACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGATTTTGAA 
GAACAGTTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATAAAA AGCATTATTC AATATGTGCA GAAATTTTGG ACCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AACGTTGACA AATAACCTGA TTCCATATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ATAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGTC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCAGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TACTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATGCGGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGATT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG CAAGTGATCC ACAAAATGTA 
TTTTCAGCTG GTCCAGTTTC AAATGAAGGA TTACCAGAAA CAGAAGGTGA AACGATACTA 
AGTGCTAATG CTGATAAAAC ACCAAAAGCT GAAGAAATCA AATCCAGTGA CAGTTTCTTA 
AAACTCACTA CGCGTGCTCA ACTTGGTGCA AAATCAGAAC AGTTGCTGAA GAAAGGAAAG 
AGCATTCCTG ACGTGCTGCT TGTTGACATC ATGGTAAATG CTATTAACGA GATACCTGTG 
AATCAGCACT GGATCCTAGA TGGTTTTCCA ATGACTTTAA ATCAAGCACA GCTTCTGGAA 
GAAGCTCTTA CAGGCTACAA TAGAAACCTC ACAGAACTGG AGAGAAAAAA AGCACAAAAA 
TCCACATTGG CTATTGATCC TGCGACTTCC AAAGAAGTAC CTCTTCCCTC TCCTGCATTT 
GATTTTGTCG TATTATTAGA CGTTTCAGAT ACTTCCTCAA TGAGTCGCAT GAAGGATATA 
ATAGCTGAAG AATTGTCCTA TAAAACTGCT CACGAAGACA TCAGTCAACG TGTAGCTGCT 
GAAAATCAAG ATAAGGGTGG AGACCAAAAT TTAAGAGACC AGATACAACA TAGAATTATA 
GGCTTCTTGG ACAACTGGCC TTTATTGAAG CAATGGTTTT CAGAGCCAGA AAATATTTTG 
ATAAAAATCA ATGCTGAAAT AGATAAGGAA TCTTTATGCG AAAAAGTAAA AGAAATTCTT 
ACGACTGAAA TAGTGAAAAA AAAGAATAAA GTTGAGAAGA AATTAGAAGA AAAGGAAGCT 
GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG CTTCCACTTC CTACACCTCC TCCTGCTCCT 
CCTCCTGAAC CAGAAAAAGA GAAGGAAATT CATCTTCATC ATGTGCCTTC AAAAACTCCT 
ACTGCAAAAG GAAAACCTCA ATCAGGTTCT CCTAAAGGAA AATCATCAGG AGGAAAAGTA 
CCAGTAAAGA AATCACCTGC TGACTCTACA GACACATCAC CTGTTGCAAT AGTGCCGCAG 
CCACCTAAGC CAGGATCAGA AGAATGGGTC TATGTGAATG AACCAGTTCC TGAGGAAATG 
CCTTCGTTTT TAGTACCTTA CTGGGAACTA ATAGAAAATT CCTATATAAA CACCATCAAA 
ACAGTACTCA GGCATCTGAG GGAAGACCAG CATACTGTGC TTGCTTACTT ATATGAGATT 
AGAACAAGTT TCCAGGAGTT TCTAAAGCGT CCAGATCACA AGCAAGATTT TGTAGCTCAA 
TGGCAGGCTG ATTTCAATTC CCTTCCTGAT GACCTGTGGG ATGATGAGGA AACAAAGGCT 
GAACTACACC AACGAGTGAA TGATCTGCGA GACCGCCTGT GGGACATTTG TGACGCACGG 
AAGGAAGAGG CAGAGCAGGA GCGGCTTGAC ATCATTAATG AGAACTGGTT ACAGGACTCT 
CTTGGAATGA CAATGAACCA TTTCTTTTCC CTGATGCAGG CAGAACTGAA CCGTTTCCAA 
GATACGAAGA GACTCCTTCA AGATTATTAC CGGGGAATGG AAAGTAAAAT CCCAATAGAG 
GACAATAAGA GATTTACTCA AATCCCTTTG GTCCAGCTGG ATAATAAAGA CAATTCTGAA 
AGCCAGCTTA GAATTCCTCT ATTTCCTCGA ATATCCATTT CTCTGGAAAC AGTTACGCCC 
AAACCAAAAA CAAAATCAAT ACTGAAGGGC AAGATGGATA ACTCCCTAGA AAACGTTGAG 
TCCAACTTTG AGGCTGATGA AAAGTTGGTC ATGGACACCT GGCAACAGGC TTCTTTAGCA 
GTATCTCACA TGGTGGCTGC AGAAATTCAT CAGAGGCTCA TGGAAGAAGA AAAAGAAAAC 
CAGCCAGCAG ACCCCAAAGA AAAATCTCCT CAGATGGGTG CAAATAAAAA AGTCAAAAAG 
GAGCCACCCA AGAAAAAACA GGAAGACAAA AAACTCAAAG GTAAATCACC ACCTATTGCA 
GAAGCAACTC CTGTCATAGT AACAACAGAG GAAATTGCTG AAATCGAAAG GAAAAATGAA 
CTGAGGCTCA AAATAAAAGA AGAACACCTT GCTGCCTTGC AATTTGAAGA AACAGCCACA 
CAGTTTCGAC TTGAACTGAT AAAGACAAAA GCATTGGCTC TTCTTGAAGA TTTAGTAACA 
AAGGTGGTTG ATGTATATAA ACTCATGGAA AAATGGCTTG GTGAGAGATA TTTGAATGAA 
ATGGCCAGTG TAGAAAAATT AACTGACATA GCTCGCTATC ACATTGAAAC ATCTACAAAA 
ATTCAGAATG AACTTTATTT AAACCAAGAA GACTTCTTCA TTAATGGCAA TGTAAAAGTC 
TTTCCAGATC CTCCCCCACC GATACATCCT CCACCTGTAG AAAAGGAAGA AGATGGCACC 
CTGACCATCG AACAGCTTGA CAATCTTCGG GATCAGTTCT TAGATATGGC ACCTAAAGGC 
AGGAATCCTT CAGATGATGT GAATAGAGTC ACCGAAAAGC AGAAAAGGCC ACAGAAGCTT 
GACCATTACC TTCCAAAAAG AAGCCATCAA TTTTTGAAAT TTTGGCTTAA AAAATATTCA 
TGGCTTATAT ATGATGAGCT ATTAAATCTT ATGTTCTGCG CCCTGTGTCG TAAGCATGGA 
GTGAAGTCAG GGGGAAGTCA AGTGAATTTC TTTTATGGTA CTGACAACTT TAGGACTCAA 
TTTCTCGATG CACATCAACT CAGCGAGGCT CATGCCAAGG CTTCATTAAT GGAAGCAACA 
AGTAGCTCTC CAGTGAACAG AGCCACCACC AAACTAATGA TGAGGACCAT GAGCAAAGTA 
ACCCTCGGGA GAGTAGAGAA CTTATTCAGA TCATGCCATG CTATTGCCAA GACCGGACGT 
ACCCTCAAAG ATTTTATTTG GATGTGCAAA TTGGATGATA TGAAAGGTGT TGATATTGGC 
CCAGTATTCA GAACTAAAAA CTCAGCAAGA ATGTTTACAT ACTTTATTGC TGAAGTAGAA 
CGAAAAAATC TAAGAGAGAA ACTAGAAAAA AGTAAATTTT TTTCTGTCAT CAGTGATGGA 
ATCACAGACA GTTTAATCAA AGAAGTCGAA CTTGTTTATG TGCAGTTTGC ACATGCAGGA 
AAAGTGCACT GTCAATTTGT TGGGGTACAG ACAGTAGAAA GAAATGATCC TCTGGCAATA 
AAAAATGCCA CTGAGAAAAC TCTAGAGATA AATCTTCAAC TTAGACTGTC GAGCCAAGAC 
TGGGCAAAGA AGCTGGTTGG TTTTGGAAGT GATGGTATCC ATGGCATAGA GGGAGAGAAC 
AATGAGGTGG CCCTGCTACT ACGAGAAATA CAACCATGTG TACAGACTGT ATATTGCTTT 
GCACATCACC TTGAACTATC TTACAAAGTG CTGTTCCAGA ACATTCCTCT GTACAATGAT 
GTCAAAGATC TCCTTCACAG CATTTATCAC TTTTATCACA ATTCTCCTCT TCATAAGAGT 
GCTCTGAGAA CTGCTTTCAA AGGCCTTCAC CTTCGACCTG TGATGCCTTC TCAAATAGGA 
GACAGGAAGT GGCTTCATGG ATTACAGGCA GCCCTCCAGA ACTTTCTCAA GGGATATCCT 
GCAATTGTTC GGCAACTGTA CTCAGCAGGC GAAGGCAAAA GTGACACCAG TCAAAAGAAA 
TCTAAAGAAC TTCTGGAGTC CCTTCTCCAA GCTGACATTG TTAAATTTGC CCACTTCTTG 
TTGGATGTCA TTAATGTCCT TAGCATTCTG TCTGGTGTCA GTAAGAATAG AAATTCCTCA 
ATTGCTGACA TATTTGCCAC TTTAGAGTCA ATGCTGGAAA TGCTCCAAAG GTATCAAACA 
AGACTAGGGC CAAAAGAACG CATGATGGAT TCAGCCACAC ACTTTCATGG TAACTGCCTC 
AGAGGGAAAG AAAACATTTC TGCTGTGAGA AACATTGTTT TAACACATCT TATCAAGAGG 
CTTCAAGGCT GTTTCAGAGA TGCCAGCCAA AATGTGGTGA GGGCTACCAT GATTGGAAGT 
TTTAAATTAT GGCCCACAAA GATAAATCAA GAATTTGGAG AAAAAGAGGT ATCCATCCTG 
ACTGCACATT ATGAACCAGT ATTAGAAGCT GCCAATGTGA AACTTAGTGA AGTGGATGCT 
GAATGGAGCA TGTTGAAATT AGAGATTTAT GCCAGATTTC CGAACATTCA AAAATTGACC 
TGGGATTTTG TGAATTCCAT TTACTTTCAC AAGTATCCAA ATATCCTGAC ACTGGTCGAC 
CTAGTGTTGA CCCTTCCTGC AAGTTCAGCT GAAGCAGAAC GTGGCTTTAG TCAGATGAAA 
TGGACCAAGT CACACATGCA TGCAAAAATC AAGACTGAAA TTATGACGGA TGTCCTAATT 
ATTCAGTTGA ATTCTCCAGA CATTAATAAT TTTGACCCTA GGAAAGCTAT CCATTTATGG 
AATACAAGAA CACTGTCTTC AACTGGTGAC ACAAGACCTA ATTCAGATTG CTCATCAAAC 
TCAGAAAGCC ATTATGAAAG TGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_007961352.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007959543.1 Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X6, mRNA.

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CloneID	OCh83462
Clone ID Related Accession (Same CDS sequence)	XM_007961352.1
Accession Version	XM_007961352.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	sperm flagellar protein 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735239.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGCAATAC 
TTAAACAGAA GACGACAAAA CGAAATAATG GCCAAAATCC AAGCAGCTAT TATACAGATT 
CCTAAACCTG CATCAAATCG TACTTTGAAA GCACTCGAGG CCCAAAAAAT GATGAAAAAG 
AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TCGAAGCATT AATAAAGAAG 
GATCTCCAAG CAAAAGAAAG TGCATCCAAG ACTACTTTAG ATACAGCAGG CCAGACAACC 
ACCGATTTGT TAAATACTTA CTCGGATGAT GAATACATTA AAAAAATCCA GAAGCGCCTT 
GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAAATT GTTAATGGAC 
CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCTTATCGGG AGGAACAGCT GATTAACCGG 
CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATTGCCGTGC AGCTCATGCA TGTTCGGCAT 
GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAACATGA GGAAAGACGA 
CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT 
GATTTTGAAG AACAGTTCCT TAAAGAAAAG AGATTTCATG ATCAGATTGC TGTGGAAAGA 
GCTCAAGCTC GTTATAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA CCAAATAGTT 
GATTTGTCCA CTAAAGTGGC AGACTATCGA ACGTTGACAA ATAACCTGAT TCCATATAAG 
TTGATGCATG ATTGGAAGGA ACTATTTTTT AATGCAAAAC CCATATATGA ACAAGCCTCT 
GTTAAGACAC TACCTGCTAA CCCCTCAAGA GAACAACTTA TAGAACTGGA GAAAAGGGAC 
TTGCTAGATA CCAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAGTG GGCCTTACCA 
GAAGAAATGG TTGACAATTT ACCACCCTCC AACAATTGCA TACTGGGTCA TATTCTTCAC 
AGGCTAGTTG AAAAATCTCT TCCTCCTCGA GCAGAATCAA CAACACCTGA ATTACCTTCA 
TTTGCTGTTA AAGGATGCTT ACTGGGGAAA ACATTAAGTG GAAAAACTAC CATTTTAAGG 
TCTCTACAAA AAGACTTTCC TATGCGGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT 
ATCCAAGCAT TTCATGACAA TGAAAAAGTC AGTGAGATTC TACCAATTCA GAAAAAAGAT 
GAAGAAGATG CTCTACCAGT TCTGCAAGAG GAGATTAAAG AAAGCCAGGC AAGTGATCCA 
CAAAATGTAT TTTCAGCTGG TCCAGTTTCA AATGAAGGAT TACCAGAAAC AGAAGGTGAA 
ACGATACTAA GTGCTAATGC TGATAAAACA CCAAAAGCTG AAGAAATCAA ATCCAGTGAC 
AGTTTCTTAA AACTCACTAC GCGTGCTCAA CTTGGTGCAA AATCAGAACA GTTGCTGAAG 
AAAGGAAAGA GCATTCCTGA CGTGCTGCTT GTTGACATCA TGGTAAATGC TATTAACGAG 
ATACCTGTGA ATCAGCACTG GATCCTAGAT GGTTTTCCAA TGACTTTAAA TCAAGCACAG 
CTTCTGGAAG AAGCTCTTAC AGGCTACAAT AGAAACCTCA CAGAACTGGA GAGAAAAAAA 
GCACAAAAAT CCACATTGGC TATTGATCCT GCGACTTCCA AAGAAGTACC TCTTCCCTCT 
CCTGCATTTG ATTTTGTCGT ATTATTAGAC GTTTCAGATA CTTCCTCAAT GAGTCGCATG 
AAGGATATAA TAGCTGAAGA ATTGTCCTAT AAAACTGCTC ACGAAGACAT CAGTCAACGT 
GTAGCTGCTG AAAATCAAGA TAAGGGTGGA GACCAAAATT TAAGAGACCA GATACAACAT 
AGAATTATAG GCTTCTTGGA CAACTGGCCT TTATTGAAGC AATGGTTTTC AGAGCCAGAA 
AATATTTTGA TAAAAATCAA TGCTGAAATA GATAAGGAAT CTTTATGCGA AAAAGTAAAA 
GAAATTCTTA CGACTGAAAT AGTGAAAAAA AAGAATAAAG TTGAGAAGAA ATTAGAAGAA 
AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC CTGGCTGAGC TTCCACTTCC TACACCTCCT 
CCTGCTCCTC CTCCTGAACC AGAAAAAGAG AAGGAAATTC ATCTTCATCA TGTGCCTTCA 
AAAACTCCTA CTGCAAAAGG AAAACCTCAA TCAGAAGCCC CGCATGGTAA GCAAGAATCT 
CTTCAGGAAG GAAAAGGGAA GAAAGGTGAA ACGGCACTCA AAAGAAAAGG TTCTCCTAAA 
GGAAAATCAT CAGGAGGAAA AGTACCAGTA AAGAAATCAC CTGCTGACTC TACAGACACA 
TCACCTGTTG CAATAGTGCC GCAGCCACCT AAGCCAGGAT CAGAAGAATG GGTCTATGTG 
AATGAACCAG TTCCTGAGGA AATGCCTTCG TTTTTAGTAC CTTACTGGGA ACTAATAGAA 
AATTCCTATA TAAACACCAT CAAAACAGTA CTCAGGCATC TGAGGGAAGA CCAGCATACT 
GTGCTTGCTT ACTTATATGA GATTAGAACA AGTTTCCAGG AGTTTCTAAA GCGTCCAGAT 
CACAAGCAAG ATTTTGTAGC TCAATGGCAG GCTGATTTCA ATTCCCTTCC TGATGACCTG 
TGGGATGATG AGGAAACAAA GGCTGAACTA CACCAACGAG TGAATGATCT GCGAGACCGC 
CTGTGGGACA TTTGTGACGC ACGGAAGGAA GAGGCAGAGC AGGAGCGGCT TGACATCATT 
AATGAGAACT GGTTACAGGA CTCTCTTGGA ATGACAATGA ACCATTTCTT TTCCCTGATG 
CAGGCAGAAC TGAACCGTTT CCAAGATACG AAGAGACTCC TTCAAGATTA TTACCGGGGA 
ATGGAAAGTA AAATCCCAAT AGAGGACAAT AAGAGATTTA CTCAAATCCC TTTGGTCCAG 
CTGGATAATA AAGACAATTC TGAAAGCCAG CTTAGAATTC CTCTATTTCC TCGAATATCC 
ATTTCTCTGG AAACAGTTAC GCCCAAACCA AAAACAAAAT CAATACTGAA GGGCAAGATG 
GATAACTCCC TAGAAAACGT TGAGTCCAAC TTTGAGGCTG ATGAAAAGTT GGTCATGGAC 
ACCTGGCAAC AGGCTTCTTT AGCAGTATCT CACATGGTGG CTGCAGAAAT TCATCAGAGG 
CTCATGGAAG AAGAAAAAGA AAACCAGCCA GCAGACCCCA AAGAAAAATC TCCTCAGATG 
GGTGCAAATA AAAAAGTCAA AAAGGAGCCA CCCAAGAAAA AACAGGAAGA CAAAAAACTC 
AAAGGTAAAT CACCACCTAT TGCAGAAGCA ACTCCTGTCA TAGTAACAAC AGAGGAAATT 
GCTGAAATCG AAAGGAAAAA TGAACTGAGG CTCAAAATAA AAGAAGAACA CCTTGCTGCC 
TTGCAATTTG AAGAAACAGC CACACAGTTT CGACTTGAAC TGATAAAGAC AAAAGCATTG 
GCTCTTCTTG AAGATTTAGT AACAAAGGTG GTTGATGTAT ATAAACTCAT GGAAAAATGG 
CTTGGTGAGA GATATTTGAA TGAAATGGCC AGTGTAGAAA AATTAACTGA CATAGCTCGC 
TATCACATTG AAACATCTAC AAAAATTCAG AATGAACTTT ATTTAAACCA AGAAGACTTC 
TTCATTAATG GCAATGTAAA AGTCTTTCCA GATCCTCCCC CACCGATACA TCCTCCACCT 
GTAGAAAAGG AAGAAGATGG CACCCTGACC ATCGAACAGC TTGACAATCT TCGGGATCAG 
TTCTTAGATA TGGCACCTAA AGGCAGGAAT CCTTCAGATG ATGTGAATAG AGTCACCGAA 
AAGCAGAAAA GGCCACAGAA GCTTGACCAT TACCTTCCAA AAAGAAGCCA TCAATTTTTG 
AAATTTTGGC TTAAAAAATA TTCATGGCTT ATATATGATG AGCTATTAAA TCTTATGTTC 
TGCGCCCTGT GTCGTAAGCA TGGAGTGAAG TCAGGGGGAA GTCAAGTGAA TTTCTTTTAT 
GGTACTGACA ACTTTAGGAC TCAATTTCTC GATGCACATC AACTCAGCGA GGCTCATGCC 
AAGGCTTCAT TAATGGAAGC AACAAGTAGC TCTCCAGTGA ACAGAGCCAC CACCAAACTA 
ATGATGAGGA CCATGAGCAA AGTAACCCTC GGGAGAGTAG AGAACTTATT CAGATCATGC 
CATGCTATTG CCAAGACCGG ACGTACCCTC AAAGATTTTA TTTGGATGTG CAAATTGGAT 
GATATGAAAG GTGTTGATAT TGGCCCAGTA TTCAGAACTA AAAACTCAGC AAGAATGTTT 
ACATACTTTA TTGCTGAAGT AGAACGAAAA AATCTAAGAG AGAAACTAGA AAAAAGTAAA 
TTTTTTTCTG TCATCAGTGA TGGAATCACA GACAGTTTAA TCAAAGAAGT CGAACTTGTT 
TATGTGCAGT TTGCACATGC AGGAAAAGTG CACTGTCAAT TTGTTGGGGT ACAGACAGTA 
GAAAGAAATG ATCCTCTGGC AATAAAAAAT GCCACTGAGA AAACTCTAGA GATAAATCTT 
CAACTTAGAC TGTCGAGCCA AGACTGGGCA AAGAAGCTGG TTGGTTTTGG AAGTGATGGT 
ATCCATGGCA TAGAGGGAGA GAACAATGAG GTGGCCCTGC TACTACGAGA AATACAACCA 
TGTGTACAGA CTGTATATTG CTTTGCACAT CACCTTGAAC TATCTTACAA AGTGCTGTTC 
CAGAACATTC CTCTGTACAA TGATGTCAAA GATCTCCTTC ACAGCATTTA TCACTTTTAT 
CACAATTCTC CTCTTCATAA GAGTGCTCTG AGAACTGCTT TCAAAGGCCT TCACCTTCGA 
CCTGTGATGC CTTCTCAAAT AGGAGACAGG AAGTGGCTTC ATGGATTACA GGCAGCCCTC 
CAGAACTTTC TCAAGGGATA TCCTGCAATT GTTCGGCAAC TGTACTCAGC AGGCGAAGGC 
AAAAGTGACA CCAGTCAAAA GAAATCTAAA GAACTTCTGG AGTCCCTTCT CCAAGCTGAC 
ATTGTTAAAT TTGCCCACTT CTTGTTGGAT GTCATTAATG TCCTTAGCAT TCTGTCTGGT 
GTCAGTAAGA ATAGAAATTC CTCAATTGCT GACATATTTG CCACTTTAGA GTCAATGCTG 
GAAATGCTCC AAAGGTATCA AACAAGACTA GGGCCAAAAG AACGCATGAT GGATTCAGCC 
ACACACTTTC ATGGTAACTG CCTCAGAGGG AAAGAAAACA TTTCTGCTGT GAGAAACATT 
GTTTTAACAC ATCTTATCAA GAGGCTTCAA GGCTGTTTCA GAGATGCCAG CCAAAATGTG 
GTGAGGGCTA CCATGATTGG AAGTTTTAAA TTATGGCCCA CAAAGATAAA TCAAGAATTT 
GGAGAAAAAG AGGTATCCAT CCTGACTGCA CATTATGAAC CAGTATTAGA AGCTGCCAAT 
GTGAAACTTA GTGAAGTGGA TGCTGAATGG AGCATGTTGA AATTAGAGAT TTATGCCAGA 
TTTCCGAACA TTCAAAAATT GACCTGGGAT TTTGTGAATT CCATTTACTT TCACAAGTAT 
CCAAATATCC TGACACTGGT CGACCTAGTG TTGACCCTTC CTGCAAGTTC AGCTGAAGCA 
GAACGTGGCT TTAGTCAGAT GAAATGGACC AAGTCACACA TGCATGCAAA AATCAAGACT 
GAAATTATGA CGGATGTCCT AATTATTCAG TTGAATTCTC CAGACATTAA TAATTTTGAC 
CCTAGGAAAG CTATCCATTT ATGGAATACA AGAACACTGT CTTCAACTGG TGACACAAGA 
CCTAATTCAG ATTGCTCATC AAACTCAGAA AGCCATTATG AAAGTGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007959543.1
CDS	99..6449
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGFLLGEVLYKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQPLTNLRLQHMKSETFREQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVANEIKKFEALIKKDLQAKESASKTTLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYKKHYSICAEILDQIVDLSTKVADYRTLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLIELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPMRILSIDTLVQEAIQAFHDNEKVSEILPIQKKDEEDALPVLQEEIKESQASDPQNVFSAGPVSNEGLPETEGETILSANADKTPKAEEIKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINEIPVNQHWILDGFPMTLNQAQLLEEALTGYNRNLTELERKKAQKSTLAIDPATSKEVPLPSPAFDFVVLLDVSDTSSMSRMKDIIAEELSYKTAHEDISQRVAAENQDKGGDQNLRDQIQHRIIGFLDNWPLLKQWFSEPENILIKINAEIDKESLCEKVKEILTTEIVKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHLHHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINENWLQDSLGMTMNHFFSLMQAELNRFQDTKRLLQDYYRGMESKIPIEDNKRFTQIPLVQLDNKDNSESQLRIPLFPRISISLETVTPKPKTKSILKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKLKGKSPPIAEATPVIVTTEEIAEIERKNELRLKIKEEHLAALQFEETATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASVEKLTDIARYHIETSTKIQNELYLNQEDFFINGNVKVFPDPPPPIHPPPVEKEEDGTLTIEQLDNLRDQFLDMAPKGRNPSDDVNRVTEKQKRPQKLDHYLPKRSHQFLKFWLKKYSWLIYDELLNLMFCALCRKHGVKSGGSQVNFFYGTDNFRTQFLDAHQLSEAHAKASLMEATSSSPVNRATTKLMMRTMSKVTLGRVENLFRSCHAIAKTGRTLKDFIWMCKLDDMKGVDIGPVFRTKNSARMFTYFIAEVERKNLREKLEKSKFFSVISDGITDSLIKEVELVYVQFAHAGKVHCQFVGVQTVERNDPLAIKNATEKTLEINLQLRLSSQDWAKKLVGFGSDGIHGIEGENNEVALLLREIQPCVQTVYCFAHHLELSYKVLFQNIPLYNDVKDLLHSIYHFYHNSPLHKSALRTAFKGLHLRPVMPSQIGDRKWLHGLQAALQNFLKGYPAIVRQLYSAGEGKSDTSQKKSKELLESLLQADIVKFAHFLLDVINVLSILSGVSKNRNSSIADIFATLESMLEMLQRYQTRLGPKERMMDSATHFHGNCLRGKENISAVRNIVLTHLIKRLQGCFRDASQNVVRATMIGSFKLWPTKINQEFGEKEVSILTAHYEPVLEAANVKLSEVDAEWSMLKLEIYARFPNIQKLTWDFVNSIYFHKYPNILTLVDLVLTLPASSAEAERGFSQMKWTKSHMHAKIKTEIMTDVLIIQLNSPDINNFDPRKAIHLWNTRTLSSTGDTRPNSDCSSNSESHYESD

Target ORF information:

RefSeq Version	XM_007961352.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus sperm flagellar 2 (SPEF2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007961352.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTTTCTAC TTGGAGAAGT TCTATACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGTTTGGA GCCAACACTT CACCTTCTGG GTGTACAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACCTCTGA CAAATTTAAG ACTTCAACAC ATGAAAAGTG AAACTTTTCG AGAGCAATAC 
TTAAACAGAA GACGACAAAA CGAAATAATG GCCAAAATCC AAGCAGCTAT TATACAGATT 
CCTAAACCTG CATCAAATCG TACTTTGAAA GCACTCGAGG CCCAAAAAAT GATGAAAAAG 
AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TCGAAGCATT AATAAAGAAG 
GATCTCCAAG CAAAAGAAAG TGCATCCAAG ACTACTTTAG ATACAGCAGG CCAGACAACC 
ACCGATTTGT TAAATACTTA CTCGGATGAT GAATACATTA AAAAAATCCA GAAGCGCCTT 
GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAAATT GTTAATGGAC 
CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCTTATCGGG AGGAACAGCT GATTAACCGG 
CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATTGCCGTGC AGCTCATGCA TGTTCGGCAT 
GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAACATGA GGAAAGACGA 
CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT 
GATTTTGAAG AACAGTTCCT TAAAGAAAAG AGATTTCATG ATCAGATTGC TGTGGAAAGA 
GCTCAAGCTC GTTATAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA CCAAATAGTT 
GATTTGTCCA CTAAAGTGGC AGACTATCGA ACGTTGACAA ATAACCTGAT TCCATATAAG 
TTGATGCATG ATTGGAAGGA ACTATTTTTT AATGCAAAAC CCATATATGA ACAAGCCTCT 
GTTAAGACAC TACCTGCTAA CCCCTCAAGA GAACAACTTA TAGAACTGGA GAAAAGGGAC 
TTGCTAGATA CCAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAGTG GGCCTTACCA 
GAAGAAATGG TTGACAATTT ACCACCCTCC AACAATTGCA TACTGGGTCA TATTCTTCAC 
AGGCTAGTTG AAAAATCTCT TCCTCCTCGA GCAGAATCAA CAACACCTGA ATTACCTTCA 
TTTGCTGTTA AAGGATGCTT ACTGGGGAAA ACATTAAGTG GAAAAACTAC CATTTTAAGG 
TCTCTACAAA AAGACTTTCC TATGCGGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT 
ATCCAAGCAT TTCATGACAA TGAAAAAGTC AGTGAGATTC TACCAATTCA GAAAAAAGAT 
GAAGAAGATG CTCTACCAGT TCTGCAAGAG GAGATTAAAG AAAGCCAGGC AAGTGATCCA 
CAAAATGTAT TTTCAGCTGG TCCAGTTTCA AATGAAGGAT TACCAGAAAC AGAAGGTGAA 
ACGATACTAA GTGCTAATGC TGATAAAACA CCAAAAGCTG AAGAAATCAA ATCCAGTGAC 
AGTTTCTTAA AACTCACTAC GCGTGCTCAA CTTGGTGCAA AATCAGAACA GTTGCTGAAG 
AAAGGAAAGA GCATTCCTGA CGTGCTGCTT GTTGACATCA TGGTAAATGC TATTAACGAG 
ATACCTGTGA ATCAGCACTG GATCCTAGAT GGTTTTCCAA TGACTTTAAA TCAAGCACAG 
CTTCTGGAAG AAGCTCTTAC AGGCTACAAT AGAAACCTCA CAGAACTGGA GAGAAAAAAA 
GCACAAAAAT CCACATTGGC TATTGATCCT GCGACTTCCA AAGAAGTACC TCTTCCCTCT 
CCTGCATTTG ATTTTGTCGT ATTATTAGAC GTTTCAGATA CTTCCTCAAT GAGTCGCATG 
AAGGATATAA TAGCTGAAGA ATTGTCCTAT AAAACTGCTC ACGAAGACAT CAGTCAACGT 
GTAGCTGCTG AAAATCAAGA TAAGGGTGGA GACCAAAATT TAAGAGACCA GATACAACAT 
AGAATTATAG GCTTCTTGGA CAACTGGCCT TTATTGAAGC AATGGTTTTC AGAGCCAGAA 
AATATTTTGA TAAAAATCAA TGCTGAAATA GATAAGGAAT CTTTATGCGA AAAAGTAAAA 
GAAATTCTTA CGACTGAAAT AGTGAAAAAA AAGAATAAAG TTGAGAAGAA ATTAGAAGAA 
AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC CTGGCTGAGC TTCCACTTCC TACACCTCCT 
CCTGCTCCTC CTCCTGAACC AGAAAAAGAG AAGGAAATTC ATCTTCATCA TGTGCCTTCA 
AAAACTCCTA CTGCAAAAGG AAAACCTCAA TCAGAAGCCC CGCATGGTAA GCAAGAATCT 
CTTCAGGAAG GAAAAGGGAA GAAAGGTGAA ACGGCACTCA AAAGAAAAGG TTCTCCTAAA 
GGAAAATCAT CAGGAGGAAA AGTACCAGTA AAGAAATCAC CTGCTGACTC TACAGACACA 
TCACCTGTTG CAATAGTGCC GCAGCCACCT AAGCCAGGAT CAGAAGAATG GGTCTATGTG 
AATGAACCAG TTCCTGAGGA AATGCCTTCG TTTTTAGTAC CTTACTGGGA ACTAATAGAA 
AATTCCTATA TAAACACCAT CAAAACAGTA CTCAGGCATC TGAGGGAAGA CCAGCATACT 
GTGCTTGCTT ACTTATATGA GATTAGAACA AGTTTCCAGG AGTTTCTAAA GCGTCCAGAT 
CACAAGCAAG ATTTTGTAGC TCAATGGCAG GCTGATTTCA ATTCCCTTCC TGATGACCTG 
TGGGATGATG AGGAAACAAA GGCTGAACTA CACCAACGAG TGAATGATCT GCGAGACCGC 
CTGTGGGACA TTTGTGACGC ACGGAAGGAA GAGGCAGAGC AGGAGCGGCT TGACATCATT 
AATGAGAACT GGTTACAGGA CTCTCTTGGA ATGACAATGA ACCATTTCTT TTCCCTGATG 
CAGGCAGAAC TGAACCGTTT CCAAGATACG AAGAGACTCC TTCAAGATTA TTACCGGGGA 
ATGGAAAGTA AAATCCCAAT AGAGGACAAT AAGAGATTTA CTCAAATCCC TTTGGTCCAG 
CTGGATAATA AAGACAATTC TGAAAGCCAG CTTAGAATTC CTCTATTTCC TCGAATATCC 
ATTTCTCTGG AAACAGTTAC GCCCAAACCA AAAACAAAAT CAATACTGAA GGGCAAGATG 
GATAACTCCC TAGAAAACGT TGAGTCCAAC TTTGAGGCTG ATGAAAAGTT GGTCATGGAC 
ACCTGGCAAC AGGCTTCTTT AGCAGTATCT CACATGGTGG CTGCAGAAAT TCATCAGAGG 
CTCATGGAAG AAGAAAAAGA AAACCAGCCA GCAGACCCCA AAGAAAAATC TCCTCAGATG 
GGTGCAAATA AAAAAGTCAA AAAGGAGCCA CCCAAGAAAA AACAGGAAGA CAAAAAACTC 
AAAGGTAAAT CACCACCTAT TGCAGAAGCA ACTCCTGTCA TAGTAACAAC AGAGGAAATT 
GCTGAAATCG AAAGGAAAAA TGAACTGAGG CTCAAAATAA AAGAAGAACA CCTTGCTGCC 
TTGCAATTTG AAGAAACAGC CACACAGTTT CGACTTGAAC TGATAAAGAC AAAAGCATTG 
GCTCTTCTTG AAGATTTAGT AACAAAGGTG GTTGATGTAT ATAAACTCAT GGAAAAATGG 
CTTGGTGAGA GATATTTGAA TGAAATGGCC AGTGTAGAAA AATTAACTGA CATAGCTCGC 
TATCACATTG AAACATCTAC AAAAATTCAG AATGAACTTT ATTTAAACCA AGAAGACTTC 
TTCATTAATG GCAATGTAAA AGTCTTTCCA GATCCTCCCC CACCGATACA TCCTCCACCT 
GTAGAAAAGG AAGAAGATGG CACCCTGACC ATCGAACAGC TTGACAATCT TCGGGATCAG 
TTCTTAGATA TGGCACCTAA AGGCAGGAAT CCTTCAGATG ATGTGAATAG AGTCACCGAA 
AAGCAGAAAA GGCCACAGAA GCTTGACCAT TACCTTCCAA AAAGAAGCCA TCAATTTTTG 
AAATTTTGGC TTAAAAAATA TTCATGGCTT ATATATGATG AGCTATTAAA TCTTATGTTC 
TGCGCCCTGT GTCGTAAGCA TGGAGTGAAG TCAGGGGGAA GTCAAGTGAA TTTCTTTTAT 
GGTACTGACA ACTTTAGGAC TCAATTTCTC GATGCACATC AACTCAGCGA GGCTCATGCC 
AAGGCTTCAT TAATGGAAGC AACAAGTAGC TCTCCAGTGA ACAGAGCCAC CACCAAACTA 
ATGATGAGGA CCATGAGCAA AGTAACCCTC GGGAGAGTAG AGAACTTATT CAGATCATGC 
CATGCTATTG CCAAGACCGG ACGTACCCTC AAAGATTTTA TTTGGATGTG CAAATTGGAT 
GATATGAAAG GTGTTGATAT TGGCCCAGTA TTCAGAACTA AAAACTCAGC AAGAATGTTT 
ACATACTTTA TTGCTGAAGT AGAACGAAAA AATCTAAGAG AGAAACTAGA AAAAAGTAAA 
TTTTTTTCTG TCATCAGTGA TGGAATCACA GACAGTTTAA TCAAAGAAGT CGAACTTGTT 
TATGTGCAGT TTGCACATGC AGGAAAAGTG CACTGTCAAT TTGTTGGGGT ACAGACAGTA 
GAAAGAAATG ATCCTCTGGC AATAAAAAAT GCCACTGAGA AAACTCTAGA GATAAATCTT 
CAACTTAGAC TGTCGAGCCA AGACTGGGCA AAGAAGCTGG TTGGTTTTGG AAGTGATGGT 
ATCCATGGCA TAGAGGGAGA GAACAATGAG GTGGCCCTGC TACTACGAGA AATACAACCA 
TGTGTACAGA CTGTATATTG CTTTGCACAT CACCTTGAAC TATCTTACAA AGTGCTGTTC 
CAGAACATTC CTCTGTACAA TGATGTCAAA GATCTCCTTC ACAGCATTTA TCACTTTTAT 
CACAATTCTC CTCTTCATAA GAGTGCTCTG AGAACTGCTT TCAAAGGCCT TCACCTTCGA 
CCTGTGATGC CTTCTCAAAT AGGAGACAGG AAGTGGCTTC ATGGATTACA GGCAGCCCTC 
CAGAACTTTC TCAAGGGATA TCCTGCAATT GTTCGGCAAC TGTACTCAGC AGGCGAAGGC 
AAAAGTGACA CCAGTCAAAA GAAATCTAAA GAACTTCTGG AGTCCCTTCT CCAAGCTGAC 
ATTGTTAAAT TTGCCCACTT CTTGTTGGAT GTCATTAATG TCCTTAGCAT TCTGTCTGGT 
GTCAGTAAGA ATAGAAATTC CTCAATTGCT GACATATTTG CCACTTTAGA GTCAATGCTG 
GAAATGCTCC AAAGGTATCA AACAAGACTA GGGCCAAAAG AACGCATGAT GGATTCAGCC 
ACACACTTTC ATGGTAACTG CCTCAGAGGG AAAGAAAACA TTTCTGCTGT GAGAAACATT 
GTTTTAACAC ATCTTATCAA GAGGCTTCAA GGCTGTTTCA GAGATGCCAG CCAAAATGTG 
GTGAGGGCTA CCATGATTGG AAGTTTTAAA TTATGGCCCA CAAAGATAAA TCAAGAATTT 
GGAGAAAAAG AGGTATCCAT CCTGACTGCA CATTATGAAC CAGTATTAGA AGCTGCCAAT 
GTGAAACTTA GTGAAGTGGA TGCTGAATGG AGCATGTTGA AATTAGAGAT TTATGCCAGA 
TTTCCGAACA TTCAAAAATT GACCTGGGAT TTTGTGAATT CCATTTACTT TCACAAGTAT 
CCAAATATCC TGACACTGGT CGACCTAGTG TTGACCCTTC CTGCAAGTTC AGCTGAAGCA 
GAACGTGGCT TTAGTCAGAT GAAATGGACC AAGTCACACA TGCATGCAAA AATCAAGACT 
GAAATTATGA CGGATGTCCT AATTATTCAG TTGAATTCTC CAGACATTAA TAATTTTGAC 
CCTAGGAAAG CTATCCATTT ATGGAATACA AGAACACTGT CTTCAACTGG TGACACAAGA 
CCTAATTCAG ATTGCTCATC AAACTCAGAA AGCCATTATG AAAGTGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SPEF2
Entrez Gene ID	103215120
Full Name	sperm flagellar 2
Gene Type	protein-coding
Organism	Chlorocebus sabaeus(green monkey)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SPEF2 cDNA ORF clone, Chlorocebus sabaeus(green monkey)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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