Dpse\GA25428 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA25428
Entrez Gene ID	6903499
Full Name	GA25428 gene product from transcript GA25428-RB
Synonyms	GA25428,dpse_GLEANR_16988
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_002132342.2	XP_002132378.2	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA25428 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA25428 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe16932	XM_002132342.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA25428), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA25428 ( XM_002132342.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_002132378.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA25428), mRNA.

Price & Availability↑

CloneID	ODe16932
Clone ID Related Accession (Same CDS sequence)	XM_002132342.2
Accession Version	XM_002132342.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589966). On Dec 22, 2015 this sequence version replaced XM_002132342.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAAGAC TTCCGTTATT CATGATGGCA ACGATATCGA TAATGTGGTT CGTGTATCAT 
GGTAGTGCCC AAAATGAGGG AAGCCAATGG GACCTTGAAG GGGATAACAT TGAACCAACC 
TCCAATCAAT CGGCACACAG CTTCATGCAA ATACAAAAAA TACCCGAAAG ATATCGTAAC 
TTTTTGAATT GGAGTAACGA GGCCCAGAAT AACATGAAGA CATTTCCTGC AAAGCGAAAT 
CCATTTCATA ATGAAGAGCA AAGAATCAAA AGACTTTGGG ATCCTGCCAA CAATGAAAGC 
CTTGATTATC GGGAAAACCA AAAAGCATTC TCCAGGAGGA GGAAACGACA AGGGAAACGT 
AGAAATATAA CGTATCCCCT TGAACCTCAT ATGGTGTTCA ATCGTCGTCC GACTTATGGT 
AAACGCTATC ACATTATCGT CATGATGCCC AGCCTAGGCG ATGAAAAATT TATAATGACC 
TTGACGGCCG ATACGAATGA TTATCTGATC GATGAGATAG TGACTGTACC CAACGGTAAG 
CCAAATCCCT GGCGTTACAC CAAAAAATTG CCATCGATAT TGAGAAAGTA TGGCATTCGT 
GTTACCAGGC TGCCGTACAG AAGTGGCTAC TACTATCACA CGCGTGCCAC TTACTTCAAC 
TCGTATCAGC GTTACGTGTA TATCATCATT AAGGCTTTTA AGAATCGGGG AAAGATTTTA 
GCACACAAGG CACGACGTGG TATCAAAATA TTACTTCCAA AATCCATGAG CTGTAACCCG 
ATGTTGACTC TGCCCTTCTG CAAGAATCCC GATGAACCTA TAGATGTCTC ACCCCGGAGT 
CATTTGCAAT TCTTTGCTGA ATTAGGAGAC GAATGCGAAA ATTTAGAATA CGATCATGTG 
GATTGGAACT TTTATGATTT GAGCGAGACA TATCTGGTTG GTCATGCGGG TATATCCAAG 
GGCCTTGTGC TCAAGATACC ACCATATAAG GTGCAATTTA AATACAATCC ACGTTTGGTA 
CGTTTTTTAT TTCTCCTGCG TGTAAATGCG ATTATAAATG GCATAAATTG TGTTGCCAGG 
TGTTACGTAC GAATGGTCGC ACCTCAAGTG GAACCGAGAA TCCGGGGTAA CATGAATCGA 
AAGGCCAATT TTGCGCGTGA GATTGTGATG GATGCCAGTA GAAGTAGAGA CAGATCGAGG 
GCACGGACAG AAGATGATTC CAGGATATTC CTTTGGGGCT GTGTCAGCGT AGATGATCCG 
AAAAATAAGT ACTGTCGCCC AAAAATGAGT ACTAAGGAAA AGATAATAAT ACCACCATAT 
TCATTGAAAC GCGGCAGTTT CTATAACTAC AGTTTGTCAA TGGTTTCGCG AATGGATTAC 
CGCGTATCTG TCACGGTATA TCAAATGCTT CAAGCTGTCA AGCATCGTAC CATTACGGCA 
CATATACTTT GCCGTCGAAA TTGCTATATG AAAACGTATG CCCCCATCGA TAGTTTCCAC 
TTGTCGGGTG AATGTGTTGA TTGTGATTCA AAGATTTATC GCTATAGATG GTATGTGACA 
GTGCAAGGAG GACAGCCAAA GATAGAGTCC AAATTTAAAA ATCTTGTAAC GCAACATACT 
GAGGCGGAGC TGCTCATACG ACTGGAGATT AGACTAAAGG ATGGCCTCAC GGGTGCCGCG 
AATTTAATAA TGAAACGAAA CGATGGACCA CAGAATGGAG AATGCACGAT ATTTCCAGTT 
AAAGGAAGGG AGGCATATAC ATACTTTTAC GTGCCTTGTA AAGGCTTCGA AACGAAATAT 
CCACCATTAC AATTTAGGTA TACAGTCGCA CTGAACGTCA TCGACTCGAA TGTACCCTAT 
CCACTGTACA AATTGACACT ACCGTCCACC AACGAGTTAC ATGTATCGAT ATGCGATTAC 
CTTGACATGT GCATCGATGT AGGTCTTAAA GTCACAGTGC TTAAAACCAA AAAAATAGGT 
GAATACAACG AAAACAATAC GCTAAAGCAT CTTTCGGATG TGAAGAACTA TATAAGTGAG 
GTGCCGTACT TGCTGTCGCG TGGTAGATGG AACAAGGCCT ACGTCAAGAC ACTGGTGGCT 
ATTCAGAAGA TTGAAACATC TGCCGAGGGC ATCTACGTCT ACGAATACTT AGACAGGGGA 
ACCATAATAA CGGGTGCACA GCTCGAACAG CTCTCAGTGT TGGCCTGTCA AGTTGTTGAA 
CGTTTGATGC CCATGGACTA TAAAGAAGCA GCCATGGTTG CTCAAATATT TGCTCGAATG 
AGCGTGGTGT TTGAGCAGAT AATCCAAGAC ATCGAATGGC TACATCGGGC AGCGTACAAC 
GCGCTCACAG AGCACCACGT CTTTTTCGTG TCCACACTGG CCTTGCCAAC AGAAAAGCAT 
CCACCGGCAC AGTGCAAGAC CCATGATGAA ATTTGCAAAT TGGGTCAGAG TTTGGGTGCA 
GAGAGGAAAT TCGAGATGGA TGTGGATCCG GCCACCCTGA TCCGCATCAA TTGGTGGTTA 
TTGGAAACAT GGTATCTGTA TAAATGTGTA TACTATCTGG GTGTTCTCGG CACCAAGCGA 
CACCATCCCT ACGATAGAGC GTTATCAACG TTCCAGGGCG GCATTTCATA TCAAATGAAT 
GTAACAGAAG TAACAGAACC TATCGAAAAT ATTACTGTAA ATACCGTCGA TGATATGTTC 
CATGTTCACA TCTCGGCGGG GCTCCTTGGG GAGCTGCGGA ACCTACTGGC TCATCCCGCA 
GTGCTCTTTC AGATCATATC GCAGCAGAAC TTTCACAACA TGTACTGGTG GTATCCGGAG 
CCATTGCCGT CGGAGACAAA TGTACTCATT GTTCATGCGT ACAGTCGCAA AAAGAACTAC 
CCTATGGGGG CGCGTTTACC GCTTAAAAAT CCGTTGAATT ATTACTTGAA CATTTCCAAA 
TTCAGTGCGA ATCCAGAGTT TGTCAAATGG ATGACCAACA GCACCATCGA GAGCCACTCT 
CACATACACG TTTATAAGAT ACCCTTGAGA TCGAAAAGTG TGTTGGCTGT GCGCATCGGT 
CATGTCACAG AGGAAATACT TATTTTCATG AGTTTGAACC AAAAACCAAA AATGCACGAA 
ATCAGGGAAA AAGCTTGCCA CATAACGCCA AACATGAAAG GTAAACGCAT TTGGATGACG 
AACAACTGCC CTGGCACTTC CATTGCCTAT GTGGGTGTAC TCAGTAGAGA GCATAAGCTC 
GAAGATGATC AAAATGATAA TAGACATCGC AAGCCGAGTA AAGTAAAAAA CTTAATTGAA 
AATGGACTCA ATTACTCGAT TCTCCTTGAG TCATATCTGT GTACATTTTG GTTGAATCGA 
TCGTTGAACC CCGGTTGGAG TACAGAATAT TGCAAAACAA AATTGGAAAA CAGGGATGAC 
ATTTTTGTCG GATGCACCTG CACCATCTTG GGATCGCTAG CCGCTCGCAT ATTTCCCGTA 
GCCGCCGAAC GAATCATTAC GGTGTTCGTC TTTCCCATAC TGGAGACAAA TTGGTATATG 
TTTGCCTTGT TTGCGATGTT ACTCCTACTG CTTTTGGTGG TACTATTAAC GTATATCGGT 
CGGATGGTCG CCCATCAGCA TCGGCGTGTC CAGCAGTGCA GAAAAAAGCA TGTGCGGTTT 
AGGGAGGACA TGAAGTTCGA TTCGGAAAAG GCAATTCTTG TACTGATAAA AACTGGCGGT 
CATGAGTTTG CTGGAACCAC TTCCAACATT AAATTGTACT TCAAATCTAA CAAGTCCAGT 
CAGTCCTCGT ACATCGTATC ACAGGATCCA GCACGACCGC GTCTCCTACG GAATAGCATC 
AATAAAATAA ATCTGCCTGC CGGTAGCGTT CAAATACCCA CCCGCCTGGC CTTTGGCATC 
GATCGGAATG GACGGTATCC GAGATGGTAT TGCCGGACGG TGAGTGTTGT CGATTTGGAG 
AATGATATGG AGCAACTGTT TATAGTCGAG AGATGGATCG AGGAGGGGCA CACGCCCTTC 
ATTCATTCCC CCTACTTCAC AAACTCGAAA AATGCAAGGC CCGTGCAGTC CTGGACGCGA 
CGTTTCCGGA ACAGTTTCGA ACAGCGTTTC CTCAATTGGT TTCTTATCAA CCCCATGACG 
GGGCCCTGGC TAAGCCGCAA TACGCTCTAT ACGATGACAC GCTTTGAACG CTCCTGCGTT 
TGGATTTGTA ATGTCAGTCT AACGATTCTC TTGGTCAGCC TTTACTTTGG ACCCTCTCCG 
TATGAGCCGA TCACCGAGGA GTCCTTCAAA GATGATAAGA CTGGTCTAAA AATGAAGGAA 
GTTCTCATTC TAGGTGTATA CTCTGTTGTT ATTGGAATTA GTGTGCTTTT GTTCTTTGAA 
TTTGCGATCA TGCGTTGTCT CTGGCGTCGG CATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002132378.2
CDS	253..4668
Translation	MQRLPLFMMATISIMWFVYHGSAQNEGSQWDLEGDNIEPTSNQSAHSFMQIQKIPERYRNFLNWSNEAQNNMKTFPAKRNPFHNEEQRIKRLWDPANNESLDYRENQKAFSRRRKRQGKRRNITYPLEPHMVFNRRPTYGKRYHIIVMMPSLGDEKFIMTLTADTNDYLIDEIVTVPNGKPNPWRYTKKLPSILRKYGIRVTRLPYRSGYYYHTRATYFNSYQRYVYIIIKAFKNRGKILAHKARRGIKILLPKSMSCNPMLTLPFCKNPDEPIDVSPRSHLQFFAELGDECENLEYDHVDWNFYDLSETYLVGHAGISKGLVLKIPPYKVQFKYNPRLVRFLFLLRVNAIINGINCVARCYVRMVAPQVEPRIRGNMNRKANFAREIVMDASRSRDRSRARTEDDSRIFLWGCVSVDDPKNKYCRPKMSTKEKIIIPPYSLKRGSFYNYSLSMVSRMDYRVSVTVYQMLQAVKHRTITAHILCRRNCYMKTYAPIDSFHLSGECVDCDSKIYRYRWYVTVQGGQPKIESKFKNLVTQHTEAELLIRLEIRLKDGLTGAANLIMKRNDGPQNGECTIFPVKGREAYTYFYVPCKGFETKYPPLQFRYTVALNVIDSNVPYPLYKLTLPSTNELHVSICDYLDMCIDVGLKVTVLKTKKIGEYNENNTLKHLSDVKNYISEVPYLLSRGRWNKAYVKTLVAIQKIETSAEGIYVYEYLDRGTIITGAQLEQLSVLACQVVERLMPMDYKEAAMVAQIFARMSVVFEQIIQDIEWLHRAAYNALTEHHVFFVSTLALPTEKHPPAQCKTHDEICKLGQSLGAERKFEMDVDPATLIRINWWLLETWYLYKCVYYLGVLGTKRHHPYDRALSTFQGGISYQMNVTEVTEPIENITVNTVDDMFHVHISAGLLGELRNLLAHPAVLFQIISQQNFHNMYWWYPEPLPSETNVLIVHAYSRKKNYPMGARLPLKNPLNYYLNISKFSANPEFVKWMTNSTIESHSHIHVYKIPLRSKSVLAVRIGHVTEEILIFMSLNQKPKMHEIREKACHITPNMKGKRIWMTNNCPGTSIAYVGVLSREHKLEDDQNDNRHRKPSKVKNLIENGLNYSILLESYLCTFWLNRSLNPGWSTEYCKTKLENRDDIFVGCTCTILGSLAARIFPVAAERIITVFVFPILETNWYMFALFAMLLLLLLVVLLTYIGRMVAHQHRRVQQCRKKHVRFREDMKFDSEKAILVLIKTGGHEFAGTTSNIKLYFKSNKSSQSSYIVSQDPARPRLLRNSINKINLPAGSVQIPTRLAFGIDRNGRYPRWYCRTVSVVDLENDMEQLFIVERWIEEGHTPFIHSPYFTNSKNARPVQSWTRRFRNSFEQRFLNWFLINPMTGPWLSRNTLYTMTRFERSCVWICNVSLTILLVSLYFGPSPYEPITEESFKDDKTGLKMKEVLILGVYSVVIGISVLLFFEFAIMRCLWRRH

Target ORF information:

RefSeq Version	XM_002132342.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA25428), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002132342.2

ORF Insert Sequence:

ATGCAAAGAC TTCCGTTATT CATGATGGCA ACGATATCGA TAATGTGGTT CGTGTATCAT 
GGTAGTGCCC AAAATGAGGG AAGCCAATGG GACCTTGAAG GGGATAACAT TGAACCAACC 
TCCAATCAAT CGGCACACAG CTTCATGCAA ATACAAAAAA TACCCGAAAG ATATCGTAAC 
TTTTTGAATT GGAGTAACGA GGCCCAGAAT AACATGAAGA CATTTCCTGC AAAGCGAAAT 
CCATTTCATA ATGAAGAGCA AAGAATCAAA AGACTTTGGG ATCCTGCCAA CAATGAAAGC 
CTTGATTATC GGGAAAACCA AAAAGCATTC TCCAGGAGGA GGAAACGACA AGGGAAACGT 
AGAAATATAA CGTATCCCCT TGAACCTCAT ATGGTGTTCA ATCGTCGTCC GACTTATGGT 
AAACGCTATC ACATTATCGT CATGATGCCC AGCCTAGGCG ATGAAAAATT TATAATGACC 
TTGACGGCCG ATACGAATGA TTATCTGATC GATGAGATAG TGACTGTACC CAACGGTAAG 
CCAAATCCCT GGCGTTACAC CAAAAAATTG CCATCGATAT TGAGAAAGTA TGGCATTCGT 
GTTACCAGGC TGCCGTACAG AAGTGGCTAC TACTATCACA CGCGTGCCAC TTACTTCAAC 
TCGTATCAGC GTTACGTGTA TATCATCATT AAGGCTTTTA AGAATCGGGG AAAGATTTTA 
GCACACAAGG CACGACGTGG TATCAAAATA TTACTTCCAA AATCCATGAG CTGTAACCCG 
ATGTTGACTC TGCCCTTCTG CAAGAATCCC GATGAACCTA TAGATGTCTC ACCCCGGAGT 
CATTTGCAAT TCTTTGCTGA ATTAGGAGAC GAATGCGAAA ATTTAGAATA CGATCATGTG 
GATTGGAACT TTTATGATTT GAGCGAGACA TATCTGGTTG GTCATGCGGG TATATCCAAG 
GGCCTTGTGC TCAAGATACC ACCATATAAG GTGCAATTTA AATACAATCC ACGTTTGGTA 
CGTTTTTTAT TTCTCCTGCG TGTAAATGCG ATTATAAATG GCATAAATTG TGTTGCCAGG 
TGTTACGTAC GAATGGTCGC ACCTCAAGTG GAACCGAGAA TCCGGGGTAA CATGAATCGA 
AAGGCCAATT TTGCGCGTGA GATTGTGATG GATGCCAGTA GAAGTAGAGA CAGATCGAGG 
GCACGGACAG AAGATGATTC CAGGATATTC CTTTGGGGCT GTGTCAGCGT AGATGATCCG 
AAAAATAAGT ACTGTCGCCC AAAAATGAGT ACTAAGGAAA AGATAATAAT ACCACCATAT 
TCATTGAAAC GCGGCAGTTT CTATAACTAC AGTTTGTCAA TGGTTTCGCG AATGGATTAC 
CGCGTATCTG TCACGGTATA TCAAATGCTT CAAGCTGTCA AGCATCGTAC CATTACGGCA 
CATATACTTT GCCGTCGAAA TTGCTATATG AAAACGTATG CCCCCATCGA TAGTTTCCAC 
TTGTCGGGTG AATGTGTTGA TTGTGATTCA AAGATTTATC GCTATAGATG GTATGTGACA 
GTGCAAGGAG GACAGCCAAA GATAGAGTCC AAATTTAAAA ATCTTGTAAC GCAACATACT 
GAGGCGGAGC TGCTCATACG ACTGGAGATT AGACTAAAGG ATGGCCTCAC GGGTGCCGCG 
AATTTAATAA TGAAACGAAA CGATGGACCA CAGAATGGAG AATGCACGAT ATTTCCAGTT 
AAAGGAAGGG AGGCATATAC ATACTTTTAC GTGCCTTGTA AAGGCTTCGA AACGAAATAT 
CCACCATTAC AATTTAGGTA TACAGTCGCA CTGAACGTCA TCGACTCGAA TGTACCCTAT 
CCACTGTACA AATTGACACT ACCGTCCACC AACGAGTTAC ATGTATCGAT ATGCGATTAC 
CTTGACATGT GCATCGATGT AGGTCTTAAA GTCACAGTGC TTAAAACCAA AAAAATAGGT 
GAATACAACG AAAACAATAC GCTAAAGCAT CTTTCGGATG TGAAGAACTA TATAAGTGAG 
GTGCCGTACT TGCTGTCGCG TGGTAGATGG AACAAGGCCT ACGTCAAGAC ACTGGTGGCT 
ATTCAGAAGA TTGAAACATC TGCCGAGGGC ATCTACGTCT ACGAATACTT AGACAGGGGA 
ACCATAATAA CGGGTGCACA GCTCGAACAG CTCTCAGTGT TGGCCTGTCA AGTTGTTGAA 
CGTTTGATGC CCATGGACTA TAAAGAAGCA GCCATGGTTG CTCAAATATT TGCTCGAATG 
AGCGTGGTGT TTGAGCAGAT AATCCAAGAC ATCGAATGGC TACATCGGGC AGCGTACAAC 
GCGCTCACAG AGCACCACGT CTTTTTCGTG TCCACACTGG CCTTGCCAAC AGAAAAGCAT 
CCACCGGCAC AGTGCAAGAC CCATGATGAA ATTTGCAAAT TGGGTCAGAG TTTGGGTGCA 
GAGAGGAAAT TCGAGATGGA TGTGGATCCG GCCACCCTGA TCCGCATCAA TTGGTGGTTA 
TTGGAAACAT GGTATCTGTA TAAATGTGTA TACTATCTGG GTGTTCTCGG CACCAAGCGA 
CACCATCCCT ACGATAGAGC GTTATCAACG TTCCAGGGCG GCATTTCATA TCAAATGAAT 
GTAACAGAAG TAACAGAACC TATCGAAAAT ATTACTGTAA ATACCGTCGA TGATATGTTC 
CATGTTCACA TCTCGGCGGG GCTCCTTGGG GAGCTGCGGA ACCTACTGGC TCATCCCGCA 
GTGCTCTTTC AGATCATATC GCAGCAGAAC TTTCACAACA TGTACTGGTG GTATCCGGAG 
CCATTGCCGT CGGAGACAAA TGTACTCATT GTTCATGCGT ACAGTCGCAA AAAGAACTAC 
CCTATGGGGG CGCGTTTACC GCTTAAAAAT CCGTTGAATT ATTACTTGAA CATTTCCAAA 
TTCAGTGCGA ATCCAGAGTT TGTCAAATGG ATGACCAACA GCACCATCGA GAGCCACTCT 
CACATACACG TTTATAAGAT ACCCTTGAGA TCGAAAAGTG TGTTGGCTGT GCGCATCGGT 
CATGTCACAG AGGAAATACT TATTTTCATG AGTTTGAACC AAAAACCAAA AATGCACGAA 
ATCAGGGAAA AAGCTTGCCA CATAACGCCA AACATGAAAG GTAAACGCAT TTGGATGACG 
AACAACTGCC CTGGCACTTC CATTGCCTAT GTGGGTGTAC TCAGTAGAGA GCATAAGCTC 
GAAGATGATC AAAATGATAA TAGACATCGC AAGCCGAGTA AAGTAAAAAA CTTAATTGAA 
AATGGACTCA ATTACTCGAT TCTCCTTGAG TCATATCTGT GTACATTTTG GTTGAATCGA 
TCGTTGAACC CCGGTTGGAG TACAGAATAT TGCAAAACAA AATTGGAAAA CAGGGATGAC 
ATTTTTGTCG GATGCACCTG CACCATCTTG GGATCGCTAG CCGCTCGCAT ATTTCCCGTA 
GCCGCCGAAC GAATCATTAC GGTGTTCGTC TTTCCCATAC TGGAGACAAA TTGGTATATG 
TTTGCCTTGT TTGCGATGTT ACTCCTACTG CTTTTGGTGG TACTATTAAC GTATATCGGT 
CGGATGGTCG CCCATCAGCA TCGGCGTGTC CAGCAGTGCA GAAAAAAGCA TGTGCGGTTT 
AGGGAGGACA TGAAGTTCGA TTCGGAAAAG GCAATTCTTG TACTGATAAA AACTGGCGGT 
CATGAGTTTG CTGGAACCAC TTCCAACATT AAATTGTACT TCAAATCTAA CAAGTCCAGT 
CAGTCCTCGT ACATCGTATC ACAGGATCCA GCACGACCGC GTCTCCTACG GAATAGCATC 
AATAAAATAA ATCTGCCTGC CGGTAGCGTT CAAATACCCA CCCGCCTGGC CTTTGGCATC 
GATCGGAATG GACGGTATCC GAGATGGTAT TGCCGGACGG TGAGTGTTGT CGATTTGGAG 
AATGATATGG AGCAACTGTT TATAGTCGAG AGATGGATCG AGGAGGGGCA CACGCCCTTC 
ATTCATTCCC CCTACTTCAC AAACTCGAAA AATGCAAGGC CCGTGCAGTC CTGGACGCGA 
CGTTTCCGGA ACAGTTTCGA ACAGCGTTTC CTCAATTGGT TTCTTATCAA CCCCATGACG 
GGGCCCTGGC TAAGCCGCAA TACGCTCTAT ACGATGACAC GCTTTGAACG CTCCTGCGTT 
TGGATTTGTA ATGTCAGTCT AACGATTCTC TTGGTCAGCC TTTACTTTGG ACCCTCTCCG 
TATGAGCCGA TCACCGAGGA GTCCTTCAAA GATGATAAGA CTGGTCTAAA AATGAAGGAA 
GTTCTCATTC TAGGTGTATA CTCTGTTGTT ATTGGAATTA GTGTGCTTTT GTTCTTTGAA 
TTTGCGATCA TGCGTTGTCT CTGGCGTCGG CATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA25428 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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