Dpse\GA15199 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA15199
Entrez Gene ID	4811846
Full Name	GA15199 gene product from transcript GA15199-RB
Synonyms	GA15199,dpse_GLEANR_10547
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001382187.3	XP_001382224.1	uncharacterized protein, isoform A
XM_015188794.1	XP_015044280.1	uncharacterized protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA15199 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA15199 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe07236	XM_001382187.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA15199), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODe27608	XM_015188794.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA15199), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA15199 ( XM_001382187.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001382224.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA15199), mRNA.

Price & Availability↑

CloneID	ODe07236
Clone ID Related Accession (Same CDS sequence)	XM_001382187.3
Accession Version	XM_001382187.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589953). On Dec 22, 2015 this sequence version replaced XM_001382187.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGTCAA CAACAATGCC AGTTAGCCCT GCTCAAGCAC AGACACAAGC AAACGTTGAT 
ACTTCAACAA GTGCTTCGTC GGTTGGGATT GGGATCGGGA TTGGATCTCC CAAAAGTGAT 
CCTTTTTCCA CAGTATCCCC TGACCAAACG AAAGCAATGT CAGACGATGC GAGTGAGAAA 
AACGGAGTAC CAAAGAATGG GAAAGATGTC ATCGCCGCAA GTGGAAGTGC GAGTGGAGTT 
GGTAACGGTA GCTCTGGAAC AGGCACAGCC GATGGACAAC CATCGTCTTC TGTAGGTTCC 
AGCAGCATCA ACTCGTGTCT GATCGCATCT CCAAACAATA CCAGTGAGCA CTCCAATAGC 
AGCAATGTAT CTGCGGTGGC TGCCCTTGCA CAAATGGGTG ATAGCGATCT TGGCCAGTCG 
CAGGAAGAGC ATTCGAAGAA AAAAAAGATG ATTGTCAAGG ATGAAGATGC GGAAAAAGCT 
TCCAATAAAG CTAAGGGCTT AGGTTCTGGA GCTGGCTCAG GATCGGGACC GGGATCGGGA 
TTGAATGCTA CCGTTGCGAT CAAAGAGGAA CCCGCCGACG TTTTAACTAG CTTGGTGAAT 
GTAAAGAAAG AAGAGAATCA CTCACCAAAT ATGTCACCTG TCGGGTTTGG TTCAATTGGA 
GGCCGATCCG GGAACACACA GGAGAGTGTG GTAACGACTG TTAAAATGGA ACTCAATGTC 
GGAAGCAGTA GCTCCGAAAA AACGACCACG GCGTTGAACA CTGACGAAAT GGGAGGAATG 
GGAGGCATTG TCTGCAATCA TTTAAGCTCT GATTTAATGA GCGAAACTTC AAACAGTACG 
CCCGGATCTA GCGGAGTGGC TTTAAACATG AACGCTCCAC ATGCCACTCC AGGAGGAGCA 
GGATGTGTAG TTGGAGTGGG AAATTTAATG GCAGGCAATG GAGGTACATC TATCGGCGTG 
GGAGTGGGAA ATTGCTTGGA TTACATGCAA CAACAGAATC ACATTTTTGT ATTTTCCACC 
CAGCTTGCAA ACAAAGGAGC TGAATCAGTT TTAAGCGGGC AATTCCAAAC TATAATTGCA 
TACCACTGCA CACAGCCAGC GACCAAAAGT TTTCTGGAGG ACTTTTTCAT GAAAAATCCG 
ATGAAAATGA ACAAATTGCA ACGGCAAAGC TCGTTAGGTA TACCCTGGAT GGGTATTGTA 
CCCGGAATCG CTACAACAGG TCCCAATCTT GTATCAAAGT TATCCCAGCA GCCAACGCCA 
CAATCGAAGT CGTCAGGCAG TCTCCAAAAG TCACAGGCGC AGTTCAATCA ATCTGATAAC 
AGCAAACGTA ATGCTATGAA TGCTCCTAGT AGCAATTATG CTGATCAAAC CGATACCCTC 
AATGAGAACG ATCTAATGTG CTGGGAGTCA GGAGCGGGAG CGGGTGGCAC CGGAAGCGGC 
TCTGGACCCA ACTCTCGAAA TAACAACGAA AGCTGCAACG GACCTAATGA GTCGCAAGCA 
ATAAAGCTCT TGGAAGCTGC GGGCGTTGAC TTAGGGCAGG GCAAGTCCAA CGATTTGAAC 
CTCAGCCACG AGAACAACAT CATATCGCTG CAAGGGGTTA AAGTGCCAGA CGAGAACCTC 
ACCCCCCAGC AACGGCAGCA TCGGGAAGAG CAGCTTGCCA AGATCAAGAA AATGAATCAA 
TTTCTCTTTC CGGAGAACGA TACTGGTAGT GGAGCAAATC AAATAATGGG CAATCAGCTG 
TCTAAGTTGC CAACCGACAT GATGATGAGC ATGGCTGGTG GAGGCAGTGC GGGTGCCGGT 
GCCACTATTG TAAATCCGCA AATGCGTCAT ATGCACTTGC CAGGAAACAT GAAGCCTGAA 
CACATAACAG GCCCTGGACT ATCCGAAGAA GTACTGCTCC CTGGAGATGT TATGACCGAC 
ATGGGAACTG CGATGTGCAA TAGCGGTCAA AAAAATAGCC TGCAGTGCGG GCCAGCACCG 
GGAGTGACAC CTGTAACTGG AGCAGGATCG ATAAACGTTA ACATGCAAGG CCCAAACTCT 
GGAGCCCCAA ATGGCAATTT GATAGGTAAT TCATCAGATT TGCTGGGTTC GTTTGGAAAC 
ACGAACTGCG GCATAGGCAT CATAGGAACT GGGCCAGATA TGTCTAAAGG AATGCCAAAT 
CAACTGCAGG ATGGGTTATC TCAGGCGCCC CAAGCGGGAA TGGCCCAAAT GGAGTGGTCC 
AAAATACAAC AGCAATTTTT CGAAGAGCGC TTGAAAGGGG GTAAAGCCAG ACCAGGGACA 
GGACCTTCAG GGCCTCTAAT AGCACAAACA CATCAGCAGT CTACCGGCAG CAATCAGGTG 
CGAAACTCCC TCCAAGGACC TCCGCCGCCA TATCATTCTA CTCAGAGATC GGCTTCGGTT 
CCGATTGCCA CTCAATCTCC AAATCCATCG AGTCCAAACA ACTTATCCCT GCCTTCTCCA 
CGAACTACTG GTGCTGCTAT GGGTTTACCG GCCAATTCAC CCATCATGGA GGGTCCGGGG 
TCAGTGCCGG TGTCGACCAC AACAGCAGCA GCGGTGGCAG CTGCGGCGGT AGCCACGTCG 
CAAGCAAACA CAATGGCGGT GCACTCAGGG TCGAAGAACT GCTTTCAGGC GGACGCGGCC 
TCGCCATCAA ATCAAAACTC GAATCCCAAT CGAAATCGAA GTAGCGGCAC ATCAGGAGTG 
CTAAATCATC TAAACAGTAA TCCGAGCACG CCACTATCTC ATCTTTCCCC GAAGGAATTG 
GACTCGTTTA ACCAATCAAA TTCTAATTCT GGAGATAATA TGAAAAATAG ACGACCTAGC 
CCACATCGTC CACGTTCTCC AGGAAATTGT CTTATAGATG CAAGTATTGA AACCCGATTT 
GCAGCTTCTA GTCCTGGCGT GAATTTTAAT CCACATCCAC ATATGCAAAG CAATACAAAT 
ACAGCATTGA ATGCCTACAA AGTATCCTCT TCAAATATTG CCATGGAGCG ACAAAATTCT 
GCGCCTGGTG GTCAGGTGCA ATTTAATCGG CGTTCTGATA ATATTCCACT GAATCCGAAC 
AGTGGTAATC GCCCGCCACC AAATAAAATG GCTCAGAACT TTGATCCAAT ATCGTCATTA 
GCACAGATGT CCCAGCAGCT TACTAGCTGT GTTTCTAGCA TGGGAAGTCC TGCCGGTGCT 
GGTGGGATGA ATATGATGGG CGGAGCCGGA CCAGGACCAG GACCGGTTGA TATAAATATT 
GATCATAGTA TGATGTCCGG CATAGATTCA ACTGGCATGG ATGCCATGAA TGCTCAAAAC 
AGTTGTCATC CCATGAATCC CTTGATGAAT CCGATGGGTC AACGTATGCT GAATCCGAAA 
ATGTGCATTC CAGGTGCCCC TCCTGGATTT AACCCGAATT CTCCCAATGG TGTTATCCGG 
GAGGGTTTAG GGCCTGGCCC TGCCACTGGA CCAGTCCCGT CACAAGGTAA TGCTCCGAAT 
TTCCATGGCA TTCTACCACC TGGTGCGCGC TTAATGGGTC GAATGCCAGT CAATTTTGGA 
TCAAACTTTA ATCCAAATAT ACAGGTAAAG GCTAGTACAC CAAATACCAT ACAGTACATG 
CCAGTCAGAC CACAGAACGC AAACAATAAC AATAATAATG GAGTAGGTAA TGTTCGAATG 
CCACCTAGCC TGGAGTTCTT GCAGAGATAC GCAAACCCGC AAATGGCTGC AGGTGGTAAT 
GTTCCACCAT CAATCTGTCC TCCACTGGGA AGTGATGGTG GAATGATTGT TGGACCAGGA 
GCAGGACCCA TGCTTATGAA CACCTCCACC GAGCAGCAGC AGCAGCAAAA CAAGATGGCA 
AACAATCCAG GTAATCCTGG AAACGGCATG GGCTTTTTCC AAAATTGCAA TCAATTAAGC 
GTCATGGAGG AAGATGCAAC GCTGGCAGGT CATGATATGG GAATGGGAAT TGGGCAACAG 
TCGATGATAC GCGGCATGCG CCCACATGGA ATGCGCCAAC ACGGAATGGG CCCCCGTTTA 
CAAGCCCCCG TCGGCAACTT AATTAATAGA CAGCAGATTC AGTTCACACA CACAGCAGAT 
GGGCAGCTTG ATTGCGGAGG CGACCCCACT GCTATTTTTA ATAACGCACC CTGTAATAGT 
TCTGGACCCG GTCCCGGTAT GTTTTCATCA TCACAACAAT CCAATCAGAC AAAACCTCTG 
CACCTGAAGT CGATGACTAG TGGGATGTGC CAAAATCCAA CGGGTGTAGG TGTCGGTGTT 
GGCTCTACAC CGGTACAAGC TCAAATACAG GCACAGGGTC AGGGCCAGTC TATAATCGGG 
CCCAGCAACA ATAACATAAT GTCAGGTGCC AGTAATAGCA CATCCAATGG CGCGACTGGC 
GTTAATTTTG TTGGTCCCTC TTCAAACGAT CTAAAGTATG CACAGCAATA TCACAGCTTC 
CAGCAACAGC TTTACGCAAC CAACACAAGG AGTCAGCAAC AACAGCAGAT GCAGCAGCAG 
CAAGGCAACA TGATAGCGAT GCCACCTAAC TTGTCACCCA ATCCGGCATT TTTTGTGAAT 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001382224.1
CDS	377..4942
Translation	MLSTTMPVSPAQAQTQANVDTSTSASSVGIGIGIGSPKSDPFSTVSPDQTKAMSDDASEKNGVPKNGKDVIAASGSASGVGNGSSGTGTADGQPSSSVGSSSINSCLIASPNNTSEHSNSSNVSAVAALAQMGDSDLGQSQEEHSKKKKMIVKDEDAEKASNKAKGLGSGAGSGSGPGSGLNATVAIKEEPADVLTSLVNVKKEENHSPNMSPVGFGSIGGRSGNTQESVVTTVKMELNVGSSSSEKTTTALNTDEMGGMGGIVCNHLSSDLMSETSNSTPGSSGVALNMNAPHATPGGAGCVVGVGNLMAGNGGTSIGVGVGNCLDYMQQQNHIFVFSTQLANKGAESVLSGQFQTIIAYHCTQPATKSFLEDFFMKNPMKMNKLQRQSSLGIPWMGIVPGIATTGPNLVSKLSQQPTPQSKSSGSLQKSQAQFNQSDNSKRNAMNAPSSNYADQTDTLNENDLMCWESGAGAGGTGSGSGPNSRNNNESCNGPNESQAIKLLEAAGVDLGQGKSNDLNLSHENNIISLQGVKVPDENLTPQQRQHREEQLAKIKKMNQFLFPENDTGSGANQIMGNQLSKLPTDMMMSMAGGGSAGAGATIVNPQMRHMHLPGNMKPEHITGPGLSEEVLLPGDVMTDMGTAMCNSGQKNSLQCGPAPGVTPVTGAGSINVNMQGPNSGAPNGNLIGNSSDLLGSFGNTNCGIGIIGTGPDMSKGMPNQLQDGLSQAPQAGMAQMEWSKIQQQFFEERLKGGKARPGTGPSGPLIAQTHQQSTGSNQVRNSLQGPPPPYHSTQRSASVPIATQSPNPSSPNNLSLPSPRTTGAAMGLPANSPIMEGPGSVPVSTTTAAAVAAAAVATSQANTMAVHSGSKNCFQADAASPSNQNSNPNRNRSSGTSGVLNHLNSNPSTPLSHLSPKELDSFNQSNSNSGDNMKNRRPSPHRPRSPGNCLIDASIETRFAASSPGVNFNPHPHMQSNTNTALNAYKVSSSNIAMERQNSAPGGQVQFNRRSDNIPLNPNSGNRPPPNKMAQNFDPISSLAQMSQQLTSCVSSMGSPAGAGGMNMMGGAGPGPGPVDINIDHSMMSGIDSTGMDAMNAQNSCHPMNPLMNPMGQRMLNPKMCIPGAPPGFNPNSPNGVIREGLGPGPATGPVPSQGNAPNFHGILPPGARLMGRMPVNFGSNFNPNIQVKASTPNTIQYMPVRPQNANNNNNNGVGNVRMPPSLEFLQRYANPQMAAGGNVPPSICPPLGSDGGMIVGPGAGPMLMNTSTEQQQQQNKMANNPGNPGNGMGFFQNCNQLSVMEEDATLAGHDMGMGIGQQSMIRGMRPHGMRQHGMGPRLQAPVGNLINRQQIQFTHTADGQLDCGGDPTAIFNNAPCNSSGPGPGMFSSSQQSNQTKPLHLKSMTSGMCQNPTGVGVGVGSTPVQAQIQAQGQGQSIIGPSNNNIMSGASNSTSNGATGVNFVGPSSNDLKYAQQYHSFQQQLYATNTRSQQQQQMQQQQGNMIAMPPNLSPNPAFFVNK

Target ORF information:

RefSeq Version	XM_001382187.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA15199), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001382187.3

ORF Insert Sequence:

ATGCTGTCAA CAACAATGCC AGTTAGCCCT GCTCAAGCAC AGACACAAGC AAACGTTGAT 
ACTTCAACAA GTGCTTCGTC GGTTGGGATT GGGATCGGGA TTGGATCTCC CAAAAGTGAT 
CCTTTTTCCA CAGTATCCCC TGACCAAACG AAAGCAATGT CAGACGATGC GAGTGAGAAA 
AACGGAGTAC CAAAGAATGG GAAAGATGTC ATCGCCGCAA GTGGAAGTGC GAGTGGAGTT 
GGTAACGGTA GCTCTGGAAC AGGCACAGCC GATGGACAAC CATCGTCTTC TGTAGGTTCC 
AGCAGCATCA ACTCGTGTCT GATCGCATCT CCAAACAATA CCAGTGAGCA CTCCAATAGC 
AGCAATGTAT CTGCGGTGGC TGCCCTTGCA CAAATGGGTG ATAGCGATCT TGGCCAGTCG 
CAGGAAGAGC ATTCGAAGAA AAAAAAGATG ATTGTCAAGG ATGAAGATGC GGAAAAAGCT 
TCCAATAAAG CTAAGGGCTT AGGTTCTGGA GCTGGCTCAG GATCGGGACC GGGATCGGGA 
TTGAATGCTA CCGTTGCGAT CAAAGAGGAA CCCGCCGACG TTTTAACTAG CTTGGTGAAT 
GTAAAGAAAG AAGAGAATCA CTCACCAAAT ATGTCACCTG TCGGGTTTGG TTCAATTGGA 
GGCCGATCCG GGAACACACA GGAGAGTGTG GTAACGACTG TTAAAATGGA ACTCAATGTC 
GGAAGCAGTA GCTCCGAAAA AACGACCACG GCGTTGAACA CTGACGAAAT GGGAGGAATG 
GGAGGCATTG TCTGCAATCA TTTAAGCTCT GATTTAATGA GCGAAACTTC AAACAGTACG 
CCCGGATCTA GCGGAGTGGC TTTAAACATG AACGCTCCAC ATGCCACTCC AGGAGGAGCA 
GGATGTGTAG TTGGAGTGGG AAATTTAATG GCAGGCAATG GAGGTACATC TATCGGCGTG 
GGAGTGGGAA ATTGCTTGGA TTACATGCAA CAACAGAATC ACATTTTTGT ATTTTCCACC 
CAGCTTGCAA ACAAAGGAGC TGAATCAGTT TTAAGCGGGC AATTCCAAAC TATAATTGCA 
TACCACTGCA CACAGCCAGC GACCAAAAGT TTTCTGGAGG ACTTTTTCAT GAAAAATCCG 
ATGAAAATGA ACAAATTGCA ACGGCAAAGC TCGTTAGGTA TACCCTGGAT GGGTATTGTA 
CCCGGAATCG CTACAACAGG TCCCAATCTT GTATCAAAGT TATCCCAGCA GCCAACGCCA 
CAATCGAAGT CGTCAGGCAG TCTCCAAAAG TCACAGGCGC AGTTCAATCA ATCTGATAAC 
AGCAAACGTA ATGCTATGAA TGCTCCTAGT AGCAATTATG CTGATCAAAC CGATACCCTC 
AATGAGAACG ATCTAATGTG CTGGGAGTCA GGAGCGGGAG CGGGTGGCAC CGGAAGCGGC 
TCTGGACCCA ACTCTCGAAA TAACAACGAA AGCTGCAACG GACCTAATGA GTCGCAAGCA 
ATAAAGCTCT TGGAAGCTGC GGGCGTTGAC TTAGGGCAGG GCAAGTCCAA CGATTTGAAC 
CTCAGCCACG AGAACAACAT CATATCGCTG CAAGGGGTTA AAGTGCCAGA CGAGAACCTC 
ACCCCCCAGC AACGGCAGCA TCGGGAAGAG CAGCTTGCCA AGATCAAGAA AATGAATCAA 
TTTCTCTTTC CGGAGAACGA TACTGGTAGT GGAGCAAATC AAATAATGGG CAATCAGCTG 
TCTAAGTTGC CAACCGACAT GATGATGAGC ATGGCTGGTG GAGGCAGTGC GGGTGCCGGT 
GCCACTATTG TAAATCCGCA AATGCGTCAT ATGCACTTGC CAGGAAACAT GAAGCCTGAA 
CACATAACAG GCCCTGGACT ATCCGAAGAA GTACTGCTCC CTGGAGATGT TATGACCGAC 
ATGGGAACTG CGATGTGCAA TAGCGGTCAA AAAAATAGCC TGCAGTGCGG GCCAGCACCG 
GGAGTGACAC CTGTAACTGG AGCAGGATCG ATAAACGTTA ACATGCAAGG CCCAAACTCT 
GGAGCCCCAA ATGGCAATTT GATAGGTAAT TCATCAGATT TGCTGGGTTC GTTTGGAAAC 
ACGAACTGCG GCATAGGCAT CATAGGAACT GGGCCAGATA TGTCTAAAGG AATGCCAAAT 
CAACTGCAGG ATGGGTTATC TCAGGCGCCC CAAGCGGGAA TGGCCCAAAT GGAGTGGTCC 
AAAATACAAC AGCAATTTTT CGAAGAGCGC TTGAAAGGGG GTAAAGCCAG ACCAGGGACA 
GGACCTTCAG GGCCTCTAAT AGCACAAACA CATCAGCAGT CTACCGGCAG CAATCAGGTG 
CGAAACTCCC TCCAAGGACC TCCGCCGCCA TATCATTCTA CTCAGAGATC GGCTTCGGTT 
CCGATTGCCA CTCAATCTCC AAATCCATCG AGTCCAAACA ACTTATCCCT GCCTTCTCCA 
CGAACTACTG GTGCTGCTAT GGGTTTACCG GCCAATTCAC CCATCATGGA GGGTCCGGGG 
TCAGTGCCGG TGTCGACCAC AACAGCAGCA GCGGTGGCAG CTGCGGCGGT AGCCACGTCG 
CAAGCAAACA CAATGGCGGT GCACTCAGGG TCGAAGAACT GCTTTCAGGC GGACGCGGCC 
TCGCCATCAA ATCAAAACTC GAATCCCAAT CGAAATCGAA GTAGCGGCAC ATCAGGAGTG 
CTAAATCATC TAAACAGTAA TCCGAGCACG CCACTATCTC ATCTTTCCCC GAAGGAATTG 
GACTCGTTTA ACCAATCAAA TTCTAATTCT GGAGATAATA TGAAAAATAG ACGACCTAGC 
CCACATCGTC CACGTTCTCC AGGAAATTGT CTTATAGATG CAAGTATTGA AACCCGATTT 
GCAGCTTCTA GTCCTGGCGT GAATTTTAAT CCACATCCAC ATATGCAAAG CAATACAAAT 
ACAGCATTGA ATGCCTACAA AGTATCCTCT TCAAATATTG CCATGGAGCG ACAAAATTCT 
GCGCCTGGTG GTCAGGTGCA ATTTAATCGG CGTTCTGATA ATATTCCACT GAATCCGAAC 
AGTGGTAATC GCCCGCCACC AAATAAAATG GCTCAGAACT TTGATCCAAT ATCGTCATTA 
GCACAGATGT CCCAGCAGCT TACTAGCTGT GTTTCTAGCA TGGGAAGTCC TGCCGGTGCT 
GGTGGGATGA ATATGATGGG CGGAGCCGGA CCAGGACCAG GACCGGTTGA TATAAATATT 
GATCATAGTA TGATGTCCGG CATAGATTCA ACTGGCATGG ATGCCATGAA TGCTCAAAAC 
AGTTGTCATC CCATGAATCC CTTGATGAAT CCGATGGGTC AACGTATGCT GAATCCGAAA 
ATGTGCATTC CAGGTGCCCC TCCTGGATTT AACCCGAATT CTCCCAATGG TGTTATCCGG 
GAGGGTTTAG GGCCTGGCCC TGCCACTGGA CCAGTCCCGT CACAAGGTAA TGCTCCGAAT 
TTCCATGGCA TTCTACCACC TGGTGCGCGC TTAATGGGTC GAATGCCAGT CAATTTTGGA 
TCAAACTTTA ATCCAAATAT ACAGGTAAAG GCTAGTACAC CAAATACCAT ACAGTACATG 
CCAGTCAGAC CACAGAACGC AAACAATAAC AATAATAATG GAGTAGGTAA TGTTCGAATG 
CCACCTAGCC TGGAGTTCTT GCAGAGATAC GCAAACCCGC AAATGGCTGC AGGTGGTAAT 
GTTCCACCAT CAATCTGTCC TCCACTGGGA AGTGATGGTG GAATGATTGT TGGACCAGGA 
GCAGGACCCA TGCTTATGAA CACCTCCACC GAGCAGCAGC AGCAGCAAAA CAAGATGGCA 
AACAATCCAG GTAATCCTGG AAACGGCATG GGCTTTTTCC AAAATTGCAA TCAATTAAGC 
GTCATGGAGG AAGATGCAAC GCTGGCAGGT CATGATATGG GAATGGGAAT TGGGCAACAG 
TCGATGATAC GCGGCATGCG CCCACATGGA ATGCGCCAAC ACGGAATGGG CCCCCGTTTA 
CAAGCCCCCG TCGGCAACTT AATTAATAGA CAGCAGATTC AGTTCACACA CACAGCAGAT 
GGGCAGCTTG ATTGCGGAGG CGACCCCACT GCTATTTTTA ATAACGCACC CTGTAATAGT 
TCTGGACCCG GTCCCGGTAT GTTTTCATCA TCACAACAAT CCAATCAGAC AAAACCTCTG 
CACCTGAAGT CGATGACTAG TGGGATGTGC CAAAATCCAA CGGGTGTAGG TGTCGGTGTT 
GGCTCTACAC CGGTACAAGC TCAAATACAG GCACAGGGTC AGGGCCAGTC TATAATCGGG 
CCCAGCAACA ATAACATAAT GTCAGGTGCC AGTAATAGCA CATCCAATGG CGCGACTGGC 
GTTAATTTTG TTGGTCCCTC TTCAAACGAT CTAAAGTATG CACAGCAATA TCACAGCTTC 
CAGCAACAGC TTTACGCAAC CAACACAAGG AGTCAGCAAC AACAGCAGAT GCAGCAGCAG 
CAAGGCAACA TGATAGCGAT GCCACCTAAC TTGTCACCCA ATCCGGCATT TTTTGTGAAT 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA15199 ( XM_015188794.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015044280.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA15199), mRNA.

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CloneID	ODe27608
Clone ID Related Accession (Same CDS sequence)	XM_015188794.1
Accession Version	XM_015188794.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589953). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGTGGT TTTGGTCGAA TTCATACGTT GAAACAAAAA TTATACATTT TCTCTTTCTG 
GCTCTTGTGA ATCTTTGTAC AAGTGTATTA GTGTTGTATA CCGAAAATCG TGAAATAAAT 
CAAAAGTTAT ATGTACATCT TAAGACTTAT GTAGACTTAT GTAGAATGCT GTCAACAACA 
ATGCCAGTTA GCCCTGCTCA AGCACAGACA CAAGCAAACG TTGATACTTC AACAAGTGCT 
TCGTCGGTTG GGATTGGGAT CGGGATTGGA TCTCCCAAAA GTGATCCTTT TTCCACAGTA 
TCCCCTGACC AAACGAAAGC AATGTCAGAC GATGCGAGTG AGAAAAACGG AGTACCAAAG 
AATGGGAAAG ATGTCATCGC CGCAAGTGGA AGTGCGAGTG GAGTTGGTAA CGGTAGCTCT 
GGAACAGGCA CAGCCGATGG ACAACCATCG TCTTCTGTAG GTTCCAGCAG CATCAACTCG 
TGTCTGATCG CATCTCCAAA CAATACCAGT GAGCACTCCA ATAGCAGCAA TGTATCTGCG 
GTGGCTGCCC TTGCACAAAT GGGTGATAGC GATCTTGGCC AGTCGCAGGA AGAGCATTCG 
AAGAAAAAAA AGATGATTGT CAAGGATGAA GATGCGGAAA AAGCTTCCAA TAAAGCTAAG 
GGCTTAGGTT CTGGAGCTGG CTCAGGATCG GGACCGGGAT CGGGATTGAA TGCTACCGTT 
GCGATCAAAG AGGAACCCGC CGACGTTTTA ACTAGCTTGG TGAATGTAAA GAAAGAAGAG 
AATCACTCAC CAAATATGTC ACCTGTCGGG TTTGGTTCAA TTGGAGGCCG ATCCGGGAAC 
ACACAGGAGA GTGTGGTAAC GACTGTTAAA ATGGAACTCA ATGTCGGAAG CAGTAGCTCC 
GAAAAAACGA CCACGGCGTT GAACACTGAC GAAATGGGAG GAATGGGAGG CATTGTCTGC 
AATCATTTAA GCTCTGATTT AATGAGCGAA ACTTCAAACA GTACGCCCGG ATCTAGCGGA 
GTGGCTTTAA ACATGAACGC TCCACATGCC ACTCCAGGAG GAGCAGGATG TGTAGTTGGA 
GTGGGAAATT TAATGGCAGG CAATGGAGGT ACATCTATCG GCGTGGGAGT GGGAAATTGC 
TTGGATTACA TGCAACAACA GAATCACATT TTTGTATTTT CCACCCAGCT TGCAAACAAA 
GGAGCTGAAT CAGTTTTAAG CGGGCAATTC CAAACTATAA TTGCATACCA CTGCACACAG 
CCAGCGACCA AAAGTTTTCT GGAGGACTTT TTCATGAAAA ATCCGATGAA AATGAACAAA 
TTGCAACGGC AAAGCTCGTT AGGTATACCC TGGATGGGTA TTGTACCCGG AATCGCTACA 
ACAGGTCCCA ATCTTGTATC AAAGTTATCC CAGCAGCCAA CGCCACAATC GAAGTCGTCA 
GGCAGTCTCC AAAAGTCACA GGCGCAGTTC AATCAATCTG ATAACAGCAA ACGTAATGCT 
ATGAATGCTC CTAGTAGCAA TTATGCTGAT CAAACCGATA CCCTCAATGA GAACGATCTA 
ATGTGCTGGG AGTCAGGAGC GGGAGCGGGT GGCACCGGAA GCGGCTCTGG ACCCAACTCT 
CGAAATAACA ACGAAAGCTG CAACGGACCT AATGAGTCGC AAGCAATAAA GCTCTTGGAA 
GCTGCGGGCG TTGACTTAGG GCAGGGCAAG TCCAACGATT TGAACCTCAG CCACGAGAAC 
AACATCATAT CGCTGCAAGG GGTTAAAGTG CCAGACGAGA ACCTCACCCC CCAGCAACGG 
CAGCATCGGG AAGAGCAGCT TGCCAAGATC AAGAAAATGA ATCAATTTCT CTTTCCGGAG 
AACGATACTG GTAGTGGAGC AAATCAAATA ATGGGCAATC AGCTGTCTAA GTTGCCAACC 
GACATGATGA TGAGCATGGC TGGTGGAGGC AGTGCGGGTG CCGGTGCCAC TATTGTAAAT 
CCGCAAATGC GTCATATGCA CTTGCCAGGA AACATGAAGC CTGAACACAT AACAGGCCCT 
GGACTATCCG AAGAAGTACT GCTCCCTGGA GATGTTATGA CCGACATGGG AACTGCGATG 
TGCAATAGCG GTCAAAAAAA TAGCCTGCAG TGCGGGCCAG CACCGGGAGT GACACCTGTA 
ACTGGAGCAG GATCGATAAA CGTTAACATG CAAGGCCCAA ACTCTGGAGC CCCAAATGGC 
AATTTGATAG GTAATTCATC AGATTTGCTG GGTTCGTTTG GAAACACGAA CTGCGGCATA 
GGCATCATAG GAACTGGGCC AGATATGTCT AAAGGAATGC CAAATCAACT GCAGGATGGG 
TTATCTCAGG CGCCCCAAGC GGGAATGGCC CAAATGGAGT GGTCCAAAAT ACAACAGCAA 
TTTTTCGAAG AGCGCTTGAA AGGGGGTAAA GCCAGACCAG GGACAGGACC TTCAGGGCCT 
CTAATAGCAC AAACACATCA GCAGTCTACC GGCAGCAATC AGGTGCGAAA CTCCCTCCAA 
GGACCTCCGC CGCCATATCA TTCTACTCAG AGATCGGCTT CGGTTCCGAT TGCCACTCAA 
TCTCCAAATC CATCGAGTCC AAACAACTTA TCCCTGCCTT CTCCACGAAC TACTGGTGCT 
GCTATGGGTT TACCGGCCAA TTCACCCATC ATGGAGGGTC CGGGGTCAGT GCCGGTGTCG 
ACCACAACAG CAGCAGCGGT GGCAGCTGCG GCGGTAGCCA CGTCGCAAGC AAACACAATG 
GCGGTGCACT CAGGGTCGAA GAACTGCTTT CAGGCGGACG CGGCCTCGCC ATCAAATCAA 
AACTCGAATC CCAATCGAAA TCGAAGTAGC GGCACATCAG GAGTGCTAAA TCATCTAAAC 
AGTAATCCGA GCACGCCACT ATCTCATCTT TCCCCGAAGG AATTGGACTC GTTTAACCAA 
TCAAATTCTA ATTCTGGAGA TAATATGAAA AATAGACGAC CTAGCCCACA TCGTCCACGT 
TCTCCAGGAA ATTGTCTTAT AGATGCAAGT ATTGAAACCC GATTTGCAGC TTCTAGTCCT 
GGCGTGAATT TTAATCCACA TCCACATATG CAAAGCAATA CAAATACAGC ATTGAATGCC 
TACAAAGTAT CCTCTTCAAA TATTGCCATG GAGCGACAAA ATTCTGCGCC TGGTGGTCAG 
GTGCAATTTA ATCGGCGTTC TGATAATATT CCACTGAATC CGAACAGTGG TAATCGCCCG 
CCACCAAATA AAATGGCTCA GAACTTTGAT CCAATATCGT CATTAGCACA GATGTCCCAG 
CAGCTTACTA GCTGTGTTTC TAGCATGGGA AGTCCTGCCG GTGCTGGTGG GATGAATATG 
ATGGGCGGAG CCGGACCAGG ACCAGGACCG GTTGATATAA ATATTGATCA TAGTATGATG 
TCCGGCATAG ATTCAACTGG CATGGATGCC ATGAATGCTC AAAACAGTTG TCATCCCATG 
AATCCCTTGA TGAATCCGAT GGGTCAACGT ATGCTGAATC CGAAAATGTG CATTCCAGGT 
GCCCCTCCTG GATTTAACCC GAATTCTCCC AATGGTGTTA TCCGGGAGGG TTTAGGGCCT 
GGCCCTGCCA CTGGACCAGT CCCGTCACAA GGTAATGCTC CGAATTTCCA TGGCATTCTA 
CCACCTGGTG CGCGCTTAAT GGGTCGAATG CCAGTCAATT TTGGATCAAA CTTTAATCCA 
AATATACAGG TAAAGGCTAG TACACCAAAT ACCATACAGT ACATGCCAGT CAGACCACAG 
AACGCAAACA ATAACAATAA TAATGGAGTA GGTAATGTTC GAATGCCACC TAGCCTGGAG 
TTCTTGCAGA GATACGCAAA CCCGCAAATG GCTGCAGGTG GTAATGTTCC ACCATCAATC 
TGTCCTCCAC TGGGAAGTGA TGGTGGAATG ATTGTTGGAC CAGGAGCAGG ACCCATGCTT 
ATGAACACCT CCACCGAGCA GCAGCAGCAG CAAAACAAGA TGGCAAACAA TCCAGGTAAT 
CCTGGAAACG GCATGGGCTT TTTCCAAAAT TGCAATCAAT TAAGCGTCAT GGAGGAAGAT 
GCAACGCTGG CAGGTCATGA TATGGGAATG GGAATTGGGC AACAGTCGAT GATACGCGGC 
ATGCGCCCAC ATGGAATGCG CCAACACGGA ATGGGCCCCC GTTTACAAGC CCCCGTCGGC 
AACTTAATTA ATAGACAGCA GATTCAGTTC ACACACACAG CAGATGGGCA GCTTGATTGC 
GGAGGCGACC CCACTGCTAT TTTTAATAAC GCACCCTGTA ATAGTTCTGG ACCCGGTCCC 
GGTATGTTTT CATCATCACA ACAATCCAAT CAGACAAAAC CTCTGCACCT GAAGTCGATG 
ACTAGTGGGA TGTGCCAAAA TCCAACGGGT GTAGGTGTCG GTGTTGGCTC TACACCGGTA 
CAAGCTCAAA TACAGGCACA GGGTCAGGGC CAGTCTATAA TCGGGCCCAG CAACAATAAC 
ATAATGTCAG GTGCCAGTAA TAGCACATCC AATGGCGCGA CTGGCGTTAA TTTTGTTGGT 
CCCTCTTCAA ACGATCTAAA GTATGCACAG CAATATCACA GCTTCCAGCA ACAGCTTTAC 
GCAACCAACA CAAGGAGTCA GCAACAACAG CAGATGCAGC AGCAGCAAGG CAACATGATA 
GCGATGCCAC CTAACTTGTC ACCCAATCCG GCATTTTTTG TGAATAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015044280.1
CDS	128..4858
Translation	MVWFWSNSYVETKIIHFLFLALVNLCTSVLVLYTENREINQKLYVHLKTYVDLCRMLSTTMPVSPAQAQTQANVDTSTSASSVGIGIGIGSPKSDPFSTVSPDQTKAMSDDASEKNGVPKNGKDVIAASGSASGVGNGSSGTGTADGQPSSSVGSSSINSCLIASPNNTSEHSNSSNVSAVAALAQMGDSDLGQSQEEHSKKKKMIVKDEDAEKASNKAKGLGSGAGSGSGPGSGLNATVAIKEEPADVLTSLVNVKKEENHSPNMSPVGFGSIGGRSGNTQESVVTTVKMELNVGSSSSEKTTTALNTDEMGGMGGIVCNHLSSDLMSETSNSTPGSSGVALNMNAPHATPGGAGCVVGVGNLMAGNGGTSIGVGVGNCLDYMQQQNHIFVFSTQLANKGAESVLSGQFQTIIAYHCTQPATKSFLEDFFMKNPMKMNKLQRQSSLGIPWMGIVPGIATTGPNLVSKLSQQPTPQSKSSGSLQKSQAQFNQSDNSKRNAMNAPSSNYADQTDTLNENDLMCWESGAGAGGTGSGSGPNSRNNNESCNGPNESQAIKLLEAAGVDLGQGKSNDLNLSHENNIISLQGVKVPDENLTPQQRQHREEQLAKIKKMNQFLFPENDTGSGANQIMGNQLSKLPTDMMMSMAGGGSAGAGATIVNPQMRHMHLPGNMKPEHITGPGLSEEVLLPGDVMTDMGTAMCNSGQKNSLQCGPAPGVTPVTGAGSINVNMQGPNSGAPNGNLIGNSSDLLGSFGNTNCGIGIIGTGPDMSKGMPNQLQDGLSQAPQAGMAQMEWSKIQQQFFEERLKGGKARPGTGPSGPLIAQTHQQSTGSNQVRNSLQGPPPPYHSTQRSASVPIATQSPNPSSPNNLSLPSPRTTGAAMGLPANSPIMEGPGSVPVSTTTAAAVAAAAVATSQANTMAVHSGSKNCFQADAASPSNQNSNPNRNRSSGTSGVLNHLNSNPSTPLSHLSPKELDSFNQSNSNSGDNMKNRRPSPHRPRSPGNCLIDASIETRFAASSPGVNFNPHPHMQSNTNTALNAYKVSSSNIAMERQNSAPGGQVQFNRRSDNIPLNPNSGNRPPPNKMAQNFDPISSLAQMSQQLTSCVSSMGSPAGAGGMNMMGGAGPGPGPVDINIDHSMMSGIDSTGMDAMNAQNSCHPMNPLMNPMGQRMLNPKMCIPGAPPGFNPNSPNGVIREGLGPGPATGPVPSQGNAPNFHGILPPGARLMGRMPVNFGSNFNPNIQVKASTPNTIQYMPVRPQNANNNNNNGVGNVRMPPSLEFLQRYANPQMAAGGNVPPSICPPLGSDGGMIVGPGAGPMLMNTSTEQQQQQNKMANNPGNPGNGMGFFQNCNQLSVMEEDATLAGHDMGMGIGQQSMIRGMRPHGMRQHGMGPRLQAPVGNLINRQQIQFTHTADGQLDCGGDPTAIFNNAPCNSSGPGPGMFSSSQQSNQTKPLHLKSMTSGMCQNPTGVGVGVGSTPVQAQIQAQGQGQSIIGPSNNNIMSGASNSTSNGATGVNFVGPSSNDLKYAQQYHSFQQQLYATNTRSQQQQQMQQQQGNMIAMPPNLSPNPAFFVNK

Target ORF information:

RefSeq Version	XM_015188794.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA15199), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015188794.1

ORF Insert Sequence:

ATGGTGTGGT TTTGGTCGAA TTCATACGTT GAAACAAAAA TTATACATTT TCTCTTTCTG 
GCTCTTGTGA ATCTTTGTAC AAGTGTATTA GTGTTGTATA CCGAAAATCG TGAAATAAAT 
CAAAAGTTAT ATGTACATCT TAAGACTTAT GTAGACTTAT GTAGAATGCT GTCAACAACA 
ATGCCAGTTA GCCCTGCTCA AGCACAGACA CAAGCAAACG TTGATACTTC AACAAGTGCT 
TCGTCGGTTG GGATTGGGAT CGGGATTGGA TCTCCCAAAA GTGATCCTTT TTCCACAGTA 
TCCCCTGACC AAACGAAAGC AATGTCAGAC GATGCGAGTG AGAAAAACGG AGTACCAAAG 
AATGGGAAAG ATGTCATCGC CGCAAGTGGA AGTGCGAGTG GAGTTGGTAA CGGTAGCTCT 
GGAACAGGCA CAGCCGATGG ACAACCATCG TCTTCTGTAG GTTCCAGCAG CATCAACTCG 
TGTCTGATCG CATCTCCAAA CAATACCAGT GAGCACTCCA ATAGCAGCAA TGTATCTGCG 
GTGGCTGCCC TTGCACAAAT GGGTGATAGC GATCTTGGCC AGTCGCAGGA AGAGCATTCG 
AAGAAAAAAA AGATGATTGT CAAGGATGAA GATGCGGAAA AAGCTTCCAA TAAAGCTAAG 
GGCTTAGGTT CTGGAGCTGG CTCAGGATCG GGACCGGGAT CGGGATTGAA TGCTACCGTT 
GCGATCAAAG AGGAACCCGC CGACGTTTTA ACTAGCTTGG TGAATGTAAA GAAAGAAGAG 
AATCACTCAC CAAATATGTC ACCTGTCGGG TTTGGTTCAA TTGGAGGCCG ATCCGGGAAC 
ACACAGGAGA GTGTGGTAAC GACTGTTAAA ATGGAACTCA ATGTCGGAAG CAGTAGCTCC 
GAAAAAACGA CCACGGCGTT GAACACTGAC GAAATGGGAG GAATGGGAGG CATTGTCTGC 
AATCATTTAA GCTCTGATTT AATGAGCGAA ACTTCAAACA GTACGCCCGG ATCTAGCGGA 
GTGGCTTTAA ACATGAACGC TCCACATGCC ACTCCAGGAG GAGCAGGATG TGTAGTTGGA 
GTGGGAAATT TAATGGCAGG CAATGGAGGT ACATCTATCG GCGTGGGAGT GGGAAATTGC 
TTGGATTACA TGCAACAACA GAATCACATT TTTGTATTTT CCACCCAGCT TGCAAACAAA 
GGAGCTGAAT CAGTTTTAAG CGGGCAATTC CAAACTATAA TTGCATACCA CTGCACACAG 
CCAGCGACCA AAAGTTTTCT GGAGGACTTT TTCATGAAAA ATCCGATGAA AATGAACAAA 
TTGCAACGGC AAAGCTCGTT AGGTATACCC TGGATGGGTA TTGTACCCGG AATCGCTACA 
ACAGGTCCCA ATCTTGTATC AAAGTTATCC CAGCAGCCAA CGCCACAATC GAAGTCGTCA 
GGCAGTCTCC AAAAGTCACA GGCGCAGTTC AATCAATCTG ATAACAGCAA ACGTAATGCT 
ATGAATGCTC CTAGTAGCAA TTATGCTGAT CAAACCGATA CCCTCAATGA GAACGATCTA 
ATGTGCTGGG AGTCAGGAGC GGGAGCGGGT GGCACCGGAA GCGGCTCTGG ACCCAACTCT 
CGAAATAACA ACGAAAGCTG CAACGGACCT AATGAGTCGC AAGCAATAAA GCTCTTGGAA 
GCTGCGGGCG TTGACTTAGG GCAGGGCAAG TCCAACGATT TGAACCTCAG CCACGAGAAC 
AACATCATAT CGCTGCAAGG GGTTAAAGTG CCAGACGAGA ACCTCACCCC CCAGCAACGG 
CAGCATCGGG AAGAGCAGCT TGCCAAGATC AAGAAAATGA ATCAATTTCT CTTTCCGGAG 
AACGATACTG GTAGTGGAGC AAATCAAATA ATGGGCAATC AGCTGTCTAA GTTGCCAACC 
GACATGATGA TGAGCATGGC TGGTGGAGGC AGTGCGGGTG CCGGTGCCAC TATTGTAAAT 
CCGCAAATGC GTCATATGCA CTTGCCAGGA AACATGAAGC CTGAACACAT AACAGGCCCT 
GGACTATCCG AAGAAGTACT GCTCCCTGGA GATGTTATGA CCGACATGGG AACTGCGATG 
TGCAATAGCG GTCAAAAAAA TAGCCTGCAG TGCGGGCCAG CACCGGGAGT GACACCTGTA 
ACTGGAGCAG GATCGATAAA CGTTAACATG CAAGGCCCAA ACTCTGGAGC CCCAAATGGC 
AATTTGATAG GTAATTCATC AGATTTGCTG GGTTCGTTTG GAAACACGAA CTGCGGCATA 
GGCATCATAG GAACTGGGCC AGATATGTCT AAAGGAATGC CAAATCAACT GCAGGATGGG 
TTATCTCAGG CGCCCCAAGC GGGAATGGCC CAAATGGAGT GGTCCAAAAT ACAACAGCAA 
TTTTTCGAAG AGCGCTTGAA AGGGGGTAAA GCCAGACCAG GGACAGGACC TTCAGGGCCT 
CTAATAGCAC AAACACATCA GCAGTCTACC GGCAGCAATC AGGTGCGAAA CTCCCTCCAA 
GGACCTCCGC CGCCATATCA TTCTACTCAG AGATCGGCTT CGGTTCCGAT TGCCACTCAA 
TCTCCAAATC CATCGAGTCC AAACAACTTA TCCCTGCCTT CTCCACGAAC TACTGGTGCT 
GCTATGGGTT TACCGGCCAA TTCACCCATC ATGGAGGGTC CGGGGTCAGT GCCGGTGTCG 
ACCACAACAG CAGCAGCGGT GGCAGCTGCG GCGGTAGCCA CGTCGCAAGC AAACACAATG 
GCGGTGCACT CAGGGTCGAA GAACTGCTTT CAGGCGGACG CGGCCTCGCC ATCAAATCAA 
AACTCGAATC CCAATCGAAA TCGAAGTAGC GGCACATCAG GAGTGCTAAA TCATCTAAAC 
AGTAATCCGA GCACGCCACT ATCTCATCTT TCCCCGAAGG AATTGGACTC GTTTAACCAA 
TCAAATTCTA ATTCTGGAGA TAATATGAAA AATAGACGAC CTAGCCCACA TCGTCCACGT 
TCTCCAGGAA ATTGTCTTAT AGATGCAAGT ATTGAAACCC GATTTGCAGC TTCTAGTCCT 
GGCGTGAATT TTAATCCACA TCCACATATG CAAAGCAATA CAAATACAGC ATTGAATGCC 
TACAAAGTAT CCTCTTCAAA TATTGCCATG GAGCGACAAA ATTCTGCGCC TGGTGGTCAG 
GTGCAATTTA ATCGGCGTTC TGATAATATT CCACTGAATC CGAACAGTGG TAATCGCCCG 
CCACCAAATA AAATGGCTCA GAACTTTGAT CCAATATCGT CATTAGCACA GATGTCCCAG 
CAGCTTACTA GCTGTGTTTC TAGCATGGGA AGTCCTGCCG GTGCTGGTGG GATGAATATG 
ATGGGCGGAG CCGGACCAGG ACCAGGACCG GTTGATATAA ATATTGATCA TAGTATGATG 
TCCGGCATAG ATTCAACTGG CATGGATGCC ATGAATGCTC AAAACAGTTG TCATCCCATG 
AATCCCTTGA TGAATCCGAT GGGTCAACGT ATGCTGAATC CGAAAATGTG CATTCCAGGT 
GCCCCTCCTG GATTTAACCC GAATTCTCCC AATGGTGTTA TCCGGGAGGG TTTAGGGCCT 
GGCCCTGCCA CTGGACCAGT CCCGTCACAA GGTAATGCTC CGAATTTCCA TGGCATTCTA 
CCACCTGGTG CGCGCTTAAT GGGTCGAATG CCAGTCAATT TTGGATCAAA CTTTAATCCA 
AATATACAGG TAAAGGCTAG TACACCAAAT ACCATACAGT ACATGCCAGT CAGACCACAG 
AACGCAAACA ATAACAATAA TAATGGAGTA GGTAATGTTC GAATGCCACC TAGCCTGGAG 
TTCTTGCAGA GATACGCAAA CCCGCAAATG GCTGCAGGTG GTAATGTTCC ACCATCAATC 
TGTCCTCCAC TGGGAAGTGA TGGTGGAATG ATTGTTGGAC CAGGAGCAGG ACCCATGCTT 
ATGAACACCT CCACCGAGCA GCAGCAGCAG CAAAACAAGA TGGCAAACAA TCCAGGTAAT 
CCTGGAAACG GCATGGGCTT TTTCCAAAAT TGCAATCAAT TAAGCGTCAT GGAGGAAGAT 
GCAACGCTGG CAGGTCATGA TATGGGAATG GGAATTGGGC AACAGTCGAT GATACGCGGC 
ATGCGCCCAC ATGGAATGCG CCAACACGGA ATGGGCCCCC GTTTACAAGC CCCCGTCGGC 
AACTTAATTA ATAGACAGCA GATTCAGTTC ACACACACAG CAGATGGGCA GCTTGATTGC 
GGAGGCGACC CCACTGCTAT TTTTAATAAC GCACCCTGTA ATAGTTCTGG ACCCGGTCCC 
GGTATGTTTT CATCATCACA ACAATCCAAT CAGACAAAAC CTCTGCACCT GAAGTCGATG 
ACTAGTGGGA TGTGCCAAAA TCCAACGGGT GTAGGTGTCG GTGTTGGCTC TACACCGGTA 
CAAGCTCAAA TACAGGCACA GGGTCAGGGC CAGTCTATAA TCGGGCCCAG CAACAATAAC 
ATAATGTCAG GTGCCAGTAA TAGCACATCC AATGGCGCGA CTGGCGTTAA TTTTGTTGGT 
CCCTCTTCAA ACGATCTAAA GTATGCACAG CAATATCACA GCTTCCAGCA ACAGCTTTAC 
GCAACCAACA CAAGGAGTCA GCAACAACAG CAGATGCAGC AGCAGCAAGG CAACATGATA 
GCGATGCCAC CTAACTTGTC ACCCAATCCG GCATTTTTTG TGAATAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
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Dpse\GA15199 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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