Dpse\GA30469 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA30469
Entrez Gene ID	4805011
Full Name	GA30469 gene product from transcript GA30469-RA
Synonyms	Dpse\GA11832,Dpse\GA15034,Dpse_GA11832,Dpse_GA15034,GA11832,GA15034,GA30469,dpse_GLEANR_16027,dpse_GLEANR_16028
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_004444311.2	XP_004444368.2	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA30469 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA30469 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe21985	XM_004444311.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA30469), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA30469 ( XM_004444311.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_004444368.2 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA30469), mRNA.

Price & Availability↑

CloneID	ODe21985
Clone ID Related Accession (Same CDS sequence)	XM_004444311.2
Accession Version	XM_004444311.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009006). On Dec 22, 2015 this sequence version replaced XM_004444311.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATATTG GCGTGGCACC AACGAAATGG GATAAATTGT CGCCGCGCGA GTTCCTGCAG 
CTGCAGGAGT TGGCCTCATA TTCCACACGC AAGCTGCAGG ATGTGCTCCG GGAGTTTTGC 
TCGCCCAGTG CCCCCTCGGC CACGCCCAAA TGCATACCAG ATGGGGACAT CGATTTCGAT 
GGGTTCAAGC GCTTCCTGGA CGCATTCCTC GACTGCGAGG CGCCGCTGGA CCTGGCCAAG 
CATTTGTTTG TTTCCTTCCT CAAGCCGACT GTCACCCAGG CCCAGCTGCA CGGGAAGGCC 
CTCAATCAAA TGGCCGCCAT CAGCAGTACG GCTGCCTGTG CACCCGTCAC GTCCCATACG 
AAGGGCTCCA TTCCGAACAT CAATAGCATT GCGGATCTGA TGCCGCAGTG CAGTGCAGGC 
GGGAGTGGAG GCGGGGGCGG AGGTGAGGTC CAGACCCAGG CACGCATCAG CTTCGTTGAT 
AAAATCCATG GCATCACCGA CAAACTACAG CACTCGCTGG GCGGACATCT CTCGCACGAT 
CCCAGCAAGA CGGGCAGCGT TCATCCGATT CTGACGGTGA CGCCCAGTCC TCTGGCCAGC 
GGTCCCAGCA TGTTCCAGGC GAGCAATCCG TCCCGCCGCT CCGTGGACTC GAGCCCCTCG 
CACTCCCAGA CGAACCACTC GCAAATGTCG CGCAATTCCT CGAAGAAGAG CAGCAATTCC 
GTTAATTGCA AAATTGATAC GGACATAAAG CTCTTGGCGC GGAAACTTTC CCATTTTGAT 
CCACTGACAC TTAAGGTTGC GCTCAAGGAT GTCGTCTGCT ATTTATCACT GCTCGAAGCT 
GGGCGGCCGG AGGACAAGCT GGAATTCATG TTCCGGCTGT ACGATACGGA CAGCAATGGA 
GTTCTGGACA CCGCCGAAAT GGACGCCATT GTCAATCAGA TGATGGCCGT GGCCGAATAC 
CTCGGCTGGG ATGTCAGCGA GCTGCGGCCG ATACTGCAAG ATATGATGGT TGAAATTGAC 
TACGACGCCG ATGGCACCGT TTCCCTGGAT GAGTGGCAGC GCGGTGGCAT GACAACCATT 
CCACTCCTCG TGCTCTTAGG TGTCGACAGC ACCACACTCA AGGAGGACGG CATTCACGTG 
TGGCGCTTGA AGCACTTCAG CAAGCCGGCT TACTGCAACC TGTGCCTCAA CATGCTGGTG 
GGCCTGGGGA AGAAGGGTCT CTGCTGTGTG TTGTGCAAAT ACACGGTCCA CGAACGATGC 
GTGCAGCATG CCCCAGCTTC CTGCATCAAC ACATACGTGA AGTCGAAAAA GCCTAAGTGC 
GGCGGGGACC TGCTACACCA CTGGGTGGAG GGCAACTGCT ACGGACGCTG CTCGAAGTGC 
CGCAAGCGCA TTAAGGCCTA CCACGGGATC ACAGGCCTCA CCTGCCGATG GTGCCACATG 
ATGCTCCACA ACCGCTGCGC CTCGTCGGTG AAGAAGGAAT GCACCCTGGG GGAGAACGCG 
GAGCTGGTTG TACCGCCCAC GGCCATCTGC CCGGCCGTCC TGGATCGTCA GCGCTCCGTC 
AATCAGGCGC ACAAGGCCAC GCATTTTCAA ATTACGCCGC CGGACGAGCT GAGTTGCCCC 
CTCCTGGTGT TCGTCAATCC CAAGAGCGGC GGCCGGCAGG GGGATCGCAT CCTGCGGAAA 
TTCCAGTACA TGCTGAACCC CCGCCAGGTG TACGACCTGT CCAAGGGTGG ACCCAAAGAG 
GGCCTCACGC TCTTCAAGGA TCTGCCCAAC TTCAAAGTCA TCTGCTGCGG CGGCGATGGC 
ACCGTGGGCT GGGTGCTCGA GGCCATGGAT TCCATCGAGC TGGCCACTCA ACCTGCCATC 
GGCGTCATTC CGTTGGGAAC GGGCAACGAC CTGGCCCGCT GCCTGCGCTG GGGCGGCGGC 
TACGAGGGCG AGAACATCCC CAAGCTGATG GATAAGATCA AGCGTGCCAC CACCGTGATG 
CTGGATCGCT GGAGCATCGA AGTGACCAAC ACACCGGCAA CGGACGAGCT CCGACCCAAG 
GTGACGCTGC ACTCGAACAT GCAGAAGGTG ATTGAGCTGT CGCAAAGTGT TGTGGTTGAC 
AAATCGCTGA TCGAACGCTT CGAGGAGATT CAGGAGATTG AGCGCCAGAG CAGCAGCAGC 
AGCACCGGCA ACATCGTTGG ACAGCAGCCA ACGGCGTCCA CATCTGCCAC ATCGATTATG 
ATGGCAACAG CCACCGAGTT CCGGCTGGCG GAGGAGGAGC AGAAGTACCA TCAGCAGCAG 
CGGCAGCAGG ATGGAGGTGG AGACAGCACC ACCACCACAA TGAGAACGAC AACCACCAAG 
AGCATTTCAA TGTCCACGTT CGAATCGAAG AGCATGAGGA CAACATTGAG GGGCACGCTG 
AGCAGCAGGA GCAGCAGTAG CAACACCAGC AGCGGTGGAA ATGTACAGCC ACAGCCATCC 
ATGGAAGGGG AGCCGGATGC TGAGGGGGAT GCGGATGGCG CGATGGTGCC GGGTGCTGGA 
GATGCAGCTG GAGATGAAGC GGAAACCCTG CAAATGCCAG CCAGAAGGAC AGATGAAACA 
GAAAAGCAAT CACTCGAGAC GCTGCTGCTG CAACACAAGC AGCAACAGCA GAAGCAGCAG 
CAACAACTTC AACAGGAATT GGCATCGGAT GCTGGAGAGG AAGTAGCAAC AGCAGCAACA 
TCATTTGGGA ATAATCAAAG CGATAATAAC AGTCAGCGCA ATAAGCAAAA CAACATCCTT 
AAACAGCAAA TTACATTGTC ATTGGATTTG TCGGACCATG ACGACGACAA CGAAGAAGAG 
GTGGACGGCA ACTGCAACAG CAACAGCACA GGCAATGGCA ATGGCAATGG CAGAGTGGCC 
CAGAACCCCA GCACACCGCA AACACCCATC ACACCCATCA CGCCGACAAC ACCGAATGCC 
CCATGCAGCA TGCCCCAGAA GCCCATCAAA GTTCAGTCTG ACAAGGACTG CACAGTGCCG 
TATAATATTA TCAACAATTA TTTCTCCGTC GGCGTGGATG CCGCCATCTG CGTCAAGTTT 
CACTTGGAGA GGGAGAAGAA CCCGCACAAA TTCAACAGCC GGATGAAGAA CAAACTATGG 
TACTTCGAAT ATGCAACGTC TGAGACGTTT GCAGCATCCT GCAAGAATCT CCACGAGAGC 
ATCGAGATTG TGTGCGACGG TGTGGCCTTG GACCTTGCCA ACGGGCCCCA TCTGCAGGGA 
GTGGCCTTGC TCAACATCCC CTACACCCAC GGCGGCTCCA ACCTGTGGGG TGAGCACCTG 
TCCCAGAAAC GGATGCGGAA GAGCGCTGGA CCCTTTGGCA AAAGCAAGAA GCTCAAGTCC 
AGCGACAAAG AGTTCTCGGC CACCAGTTTC AACTCCGTGG ATCTTTCGGT GGCTATACAG 
GATATCGGTG ATCGCCTGAT CGAAGTTATT GGCCTCGAGA ACTGTCTGCA CATGGGCCAG 
GTGCGGACGG GTCTGCGCGC CTCCGGTCGC CGTCTGGCGC AGTGCAGTGA GGTCATAATC 
AAGACCAAAA AGACCTTTCC CATGCAGATC GACGGCGAGC CCTGGATGCA GATGCCGTGC 
ACGATTAAGG TGACCCACAA GAACCAGGTG CCCATGCTGA TGGCCGCCAG GTCGGAGAAA 
GGACGCGGCT TCTTCAATAT GCTGTGCAGC TGATTCAGGC GCAGCGCATC GACCGACTTC 
ATGGGCCGAC AGGCCGCAGA GTCAGCCGAT GAGCTGGATG ACGATGAATT TCGGATCGTG 
ACTAGGAGCA CCACCGTCCA TAACATAGCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004444368.2
CDS	628..4440
Translation	MNIGVAPTKWDKLSPREFLQLQELASYSTRKLQDVLREFCSPSAPSATPKCIPDGDIDFDGFKRFLDAFLDCEAPLDLAKHLFVSFLKPTVTQAQLHGKALNQMAAISSTAACAPVTSHTKGSIPNINSIADLMPQCSAGGSGGGGGGEVQTQARISFVDKIHGITDKLQHSLGGHLSHDPSKTGSVHPILTVTPSPLASGPSMFQASNPSRRSVDSSPSHSQTNHSQMSRNSSKKSSNSVNCKIDTDIKLLARKLSHFDPLTLKVALKDVVCYLSLLEAGRPEDKLEFMFRLYDTDSNGVLDTAEMDAIVNQMMAVAEYLGWDVSELRPILQDMMVEIDYDADGTVSLDEWQRGGMTTIPLLVLLGVDSTTLKEDGIHVWRLKHFSKPAYCNLCLNMLVGLGKKGLCCVLCKYTVHERCVQHAPASCINTYVKSKKPKCGGDLLHHWVEGNCYGRCSKCRKRIKAYHGITGLTCRWCHMMLHNRCASSVKKECTLGENAELVVPPTAICPAVLDRQRSVNQAHKATHFQITPPDELSCPLLVFVNPKSGGRQGDRILRKFQYMLNPRQVYDLSKGGPKEGLTLFKDLPNFKVICCGGDGTVGWVLEAMDSIELATQPAIGVIPLGTGNDLARCLRWGGGYEGENIPKLMDKIKRATTVMLDRWSIEVTNTPATDELRPKVTLHSNMQKVIELSQSVVVDKSLIERFEEIQEIERQSSSSSTGNIVGQQPTASTSATSIMMATATEFRLAEEEQKYHQQQRQQDGGGDSTTTTMRTTTTKSISMSTFESKSMRTTLRGTLSSRSSSSNTSSGGNVQPQPSMEGEPDAEGDADGAMVPGAGDAAGDEAETLQMPARRTDETEKQSLETLLLQHKQQQQKQQQQLQQELASDAGEEVATAATSFGNNQSDNNSQRNKQNNILKQQITLSLDLSDHDDDNEEEVDGNCNSNSTGNGNGNGRVAQNPSTPQTPITPITPTTPNAPCSMPQKPIKVQSDKDCTVPYNIINNYFSVGVDAAICVKFHLEREKNPHKFNSRMKNKLWYFEYATSETFAASCKNLHESIEIVCDGVALDLANGPHLQGVALLNIPYTHGGSNLWGEHLSQKRMRKSAGPFGKSKKLKSSDKEFSATSFNSVDLSVAIQDIGDRLIEVIGLENCLHMGQVRTGLRASGRRLAQCSEVIIKTKKTFPMQIDGEPWMQMPCTIKVTHKNQVPMLMAARSEKGRGFFNMLCS*FRRSASTDFMGRQAAESADELDDDEFRIVTRSTTVHNIA

Target ORF information:

RefSeq Version	XM_004444311.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA30469), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004444311.2

ORF Insert Sequence:

ATGAATATTG GCGTGGCACC AACGAAATGG GATAAATTGT CGCCGCGCGA GTTCCTGCAG 
CTGCAGGAGT TGGCCTCATA TTCCACACGC AAGCTGCAGG ATGTGCTCCG GGAGTTTTGC 
TCGCCCAGTG CCCCCTCGGC CACGCCCAAA TGCATACCAG ATGGGGACAT CGATTTCGAT 
GGGTTCAAGC GCTTCCTGGA CGCATTCCTC GACTGCGAGG CGCCGCTGGA CCTGGCCAAG 
CATTTGTTTG TTTCCTTCCT CAAGCCGACT GTCACCCAGG CCCAGCTGCA CGGGAAGGCC 
CTCAATCAAA TGGCCGCCAT CAGCAGTACG GCTGCCTGTG CACCCGTCAC GTCCCATACG 
AAGGGCTCCA TTCCGAACAT CAATAGCATT GCGGATCTGA TGCCGCAGTG CAGTGCAGGC 
GGGAGTGGAG GCGGGGGCGG AGGTGAGGTC CAGACCCAGG CACGCATCAG CTTCGTTGAT 
AAAATCCATG GCATCACCGA CAAACTACAG CACTCGCTGG GCGGACATCT CTCGCACGAT 
CCCAGCAAGA CGGGCAGCGT TCATCCGATT CTGACGGTGA CGCCCAGTCC TCTGGCCAGC 
GGTCCCAGCA TGTTCCAGGC GAGCAATCCG TCCCGCCGCT CCGTGGACTC GAGCCCCTCG 
CACTCCCAGA CGAACCACTC GCAAATGTCG CGCAATTCCT CGAAGAAGAG CAGCAATTCC 
GTTAATTGCA AAATTGATAC GGACATAAAG CTCTTGGCGC GGAAACTTTC CCATTTTGAT 
CCACTGACAC TTAAGGTTGC GCTCAAGGAT GTCGTCTGCT ATTTATCACT GCTCGAAGCT 
GGGCGGCCGG AGGACAAGCT GGAATTCATG TTCCGGCTGT ACGATACGGA CAGCAATGGA 
GTTCTGGACA CCGCCGAAAT GGACGCCATT GTCAATCAGA TGATGGCCGT GGCCGAATAC 
CTCGGCTGGG ATGTCAGCGA GCTGCGGCCG ATACTGCAAG ATATGATGGT TGAAATTGAC 
TACGACGCCG ATGGCACCGT TTCCCTGGAT GAGTGGCAGC GCGGTGGCAT GACAACCATT 
CCACTCCTCG TGCTCTTAGG TGTCGACAGC ACCACACTCA AGGAGGACGG CATTCACGTG 
TGGCGCTTGA AGCACTTCAG CAAGCCGGCT TACTGCAACC TGTGCCTCAA CATGCTGGTG 
GGCCTGGGGA AGAAGGGTCT CTGCTGTGTG TTGTGCAAAT ACACGGTCCA CGAACGATGC 
GTGCAGCATG CCCCAGCTTC CTGCATCAAC ACATACGTGA AGTCGAAAAA GCCTAAGTGC 
GGCGGGGACC TGCTACACCA CTGGGTGGAG GGCAACTGCT ACGGACGCTG CTCGAAGTGC 
CGCAAGCGCA TTAAGGCCTA CCACGGGATC ACAGGCCTCA CCTGCCGATG GTGCCACATG 
ATGCTCCACA ACCGCTGCGC CTCGTCGGTG AAGAAGGAAT GCACCCTGGG GGAGAACGCG 
GAGCTGGTTG TACCGCCCAC GGCCATCTGC CCGGCCGTCC TGGATCGTCA GCGCTCCGTC 
AATCAGGCGC ACAAGGCCAC GCATTTTCAA ATTACGCCGC CGGACGAGCT GAGTTGCCCC 
CTCCTGGTGT TCGTCAATCC CAAGAGCGGC GGCCGGCAGG GGGATCGCAT CCTGCGGAAA 
TTCCAGTACA TGCTGAACCC CCGCCAGGTG TACGACCTGT CCAAGGGTGG ACCCAAAGAG 
GGCCTCACGC TCTTCAAGGA TCTGCCCAAC TTCAAAGTCA TCTGCTGCGG CGGCGATGGC 
ACCGTGGGCT GGGTGCTCGA GGCCATGGAT TCCATCGAGC TGGCCACTCA ACCTGCCATC 
GGCGTCATTC CGTTGGGAAC GGGCAACGAC CTGGCCCGCT GCCTGCGCTG GGGCGGCGGC 
TACGAGGGCG AGAACATCCC CAAGCTGATG GATAAGATCA AGCGTGCCAC CACCGTGATG 
CTGGATCGCT GGAGCATCGA AGTGACCAAC ACACCGGCAA CGGACGAGCT CCGACCCAAG 
GTGACGCTGC ACTCGAACAT GCAGAAGGTG ATTGAGCTGT CGCAAAGTGT TGTGGTTGAC 
AAATCGCTGA TCGAACGCTT CGAGGAGATT CAGGAGATTG AGCGCCAGAG CAGCAGCAGC 
AGCACCGGCA ACATCGTTGG ACAGCAGCCA ACGGCGTCCA CATCTGCCAC ATCGATTATG 
ATGGCAACAG CCACCGAGTT CCGGCTGGCG GAGGAGGAGC AGAAGTACCA TCAGCAGCAG 
CGGCAGCAGG ATGGAGGTGG AGACAGCACC ACCACCACAA TGAGAACGAC AACCACCAAG 
AGCATTTCAA TGTCCACGTT CGAATCGAAG AGCATGAGGA CAACATTGAG GGGCACGCTG 
AGCAGCAGGA GCAGCAGTAG CAACACCAGC AGCGGTGGAA ATGTACAGCC ACAGCCATCC 
ATGGAAGGGG AGCCGGATGC TGAGGGGGAT GCGGATGGCG CGATGGTGCC GGGTGCTGGA 
GATGCAGCTG GAGATGAAGC GGAAACCCTG CAAATGCCAG CCAGAAGGAC AGATGAAACA 
GAAAAGCAAT CACTCGAGAC GCTGCTGCTG CAACACAAGC AGCAACAGCA GAAGCAGCAG 
CAACAACTTC AACAGGAATT GGCATCGGAT GCTGGAGAGG AAGTAGCAAC AGCAGCAACA 
TCATTTGGGA ATAATCAAAG CGATAATAAC AGTCAGCGCA ATAAGCAAAA CAACATCCTT 
AAACAGCAAA TTACATTGTC ATTGGATTTG TCGGACCATG ACGACGACAA CGAAGAAGAG 
GTGGACGGCA ACTGCAACAG CAACAGCACA GGCAATGGCA ATGGCAATGG CAGAGTGGCC 
CAGAACCCCA GCACACCGCA AACACCCATC ACACCCATCA CGCCGACAAC ACCGAATGCC 
CCATGCAGCA TGCCCCAGAA GCCCATCAAA GTTCAGTCTG ACAAGGACTG CACAGTGCCG 
TATAATATTA TCAACAATTA TTTCTCCGTC GGCGTGGATG CCGCCATCTG CGTCAAGTTT 
CACTTGGAGA GGGAGAAGAA CCCGCACAAA TTCAACAGCC GGATGAAGAA CAAACTATGG 
TACTTCGAAT ATGCAACGTC TGAGACGTTT GCAGCATCCT GCAAGAATCT CCACGAGAGC 
ATCGAGATTG TGTGCGACGG TGTGGCCTTG GACCTTGCCA ACGGGCCCCA TCTGCAGGGA 
GTGGCCTTGC TCAACATCCC CTACACCCAC GGCGGCTCCA ACCTGTGGGG TGAGCACCTG 
TCCCAGAAAC GGATGCGGAA GAGCGCTGGA CCCTTTGGCA AAAGCAAGAA GCTCAAGTCC 
AGCGACAAAG AGTTCTCGGC CACCAGTTTC AACTCCGTGG ATCTTTCGGT GGCTATACAG 
GATATCGGTG ATCGCCTGAT CGAAGTTATT GGCCTCGAGA ACTGTCTGCA CATGGGCCAG 
GTGCGGACGG GTCTGCGCGC CTCCGGTCGC CGTCTGGCGC AGTGCAGTGA GGTCATAATC 
AAGACCAAAA AGACCTTTCC CATGCAGATC GACGGCGAGC CCTGGATGCA GATGCCGTGC 
ACGATTAAGG TGACCCACAA GAACCAGGTG CCCATGCTGA TGGCCGCCAG GTCGGAGAAA 
GGACGCGGCT TCTTCAATAT GCTGTGCAGC TGATTCAGGC GCAGCGCATC GACCGACTTC 
ATGGGCCGAC AGGCCGCAGA GTCAGCCGAT GAGCTGGATG ACGATGAATT TCGGATCGTG 
ACTAGGAGCA CCACCGTCCA TAACATAGCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA30469 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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